LOCUS       DAOOOL010000001        70290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_17_length_70290_cov_115.710999, whole genome shotgun sequence.
ACCESSION   DAOOOL010000001 DAOOOL010000000
VERSION     DAOOOL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 70290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70290
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17_length_70290_cov_115.710999"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKU82_00005"
     CDS             <1..380
                     /locus_tag="PKU82_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOZ81284.1"
                     /translation="VASLLKIQSIEVYEVATFYSMFNMKPVGKCLIEVCRTGPCWLMG
                     AEDVVRYIEKKLGIKAGETTADGMFTLKTVECLASCGTAPMMQIGETYHENLTLEKVD
                     DILNDHKNQGNRRTHWEKDKVKP"
     gene            391..1728
                     /gene="nuoF"
                     /locus_tag="PKU82_00010"
     CDS             391..1728
                     /gene="nuoF"
                     /locus_tag="PKU82_00010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOZ81285.1"
                     /translation="MSRKLLLDNLDKPGIQSITGYSDNGGYEALRKALQMKPEEIVSE
                     VQKSGLRGRGGAGFPTGMKWSFIAKPEGVPRYLVCNADESEPGTFKDRYLMEHKPHIL
                     IEGMIISSFALGANTSYIYIRGEMMYVFHILQKAIDEAYAAGYLGKNILGSGFNLDLH
                     VHCGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGLWGCPTVVNNVETIAAVVPI
                     IKITGEEYAKIGIGKSTGTKLISASGHINKPGVYEIELGVPVEEFIYSDAYCGGIKNG
                     KQLKAVVAGGSSVPVLPAHLILKTAAGEPRLMTYESLADGGFATGTMLGSGGFIVMDE
                     DTSVVKNLWTFAHFYHHESCGQCSPCREGTGWMYRILNKILNGKGEMKDIDLLWNIQG
                     NIEGKTICPLGDAAAWPVASSIRHFRSEFEAYINNHGMKDVKHYYRLNNLQEA"
     gene            1728..2702
                     /locus_tag="PKU82_00015"
     CDS             1728..2702
                     /locus_tag="PKU82_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOZ81286.1"
                     /translation="MPKVTIDGHTIEVPEGTTILEAARKIGGDIVPPTMCYYSKLKTS
                     GGYCRTCLVKVSKGSEKDPRPMPKPVASCRQTVMDGMEVQNITSPEILDARAGVVEFL
                     LINHPLDCPVCDQAGECNLQDLSYEHGHEVSRLDFRKRTFPMIDIGDKIKLHMNRCIM
                     CYRCVKVAEQLCPERVHGVLNRGWDSEISTYIEQAVDNEWSGNMIDVCPVGALTDKTF
                     RFKSRVWFTKPVDAHRDCPTCSGKVVLWYQGEEVLRVTGRKDQWGEVDEFICNECRFD
                     KKKTSDWVIEGPRHIDRHSVISQNHYEELKKLKQDLSRQKKLGEKAHN"
     gene            2712..3752
                     /gene="nuoH"
                     /locus_tag="PKU82_00020"
     CDS             2712..3752
                     /gene="nuoH"
                     /locus_tag="PKU82_00020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOZ81287.1"
                     /translation="MEVSVLIFKTVFVIAVFLVSLLIAMYSTYAERKVAAFLQDRVGP
                     NRAGPFGILQPLADGVKMFMKEEIIPSHADKALFILGPSLMMLTACMTGVVIPWGDTL
                     IINGKEYFMHITDVNIGVLYIFGVVSIGVYGIMIGGWASNNKYSLLGSVRASSQMISY
                     ELAMGMSIIALIMVTGTLSVREIVVQQQGFHWNVFYQPLGFLIFLICAFAECNRTPFD
                     LPECETELVGGYHTEYSSMKLGFYLFAEYINMFISSAVISALYFGGFNMPFISELGLS
                     HNALTLLGVLFYFIKIFFFIFFFMWVRWTIPRFRYDQLMNLGWKMLIPLSILNVLLTG
                     VFLLWKNGLFHF"
     gene            3767..4336
                     /locus_tag="PKU82_00025"
     CDS             3767..4336
                     /locus_tag="PKU82_00025"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ81288.1"
                     /translation="MTKELLIVGGPNGSGKTTFAKSFLQEYKYEFLNADEIARELSND
                     GSRAGNISSGKEYFKRIEKLKAKNKNIILESTLSGLFLRKLIREFKKKDYSIKIVFIV
                     LNAPEQCIGRIKHRVETGGHFVPDDDVRRRFTRGKNNFWKIYKDLVEDWKLFNNTESG
                     FEIIAAGEKNKYDIVNQSLFDLFIKDIEK"
     gene            4333..4548
                     /locus_tag="PKU82_00030"
     CDS             4333..4548
                     /locus_tag="PKU82_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81289.1"
                     /translation="MKSSKFKNYKNSNLFKEALEFAAIGNRGVQLAMIDSKKRKIPTV
                     FGIMDMVFYRLPDGSITTKSPFKKRKK"
     gene            4590..5135
                     /locus_tag="PKU82_00035"
     CDS             4590..5135
                     /locus_tag="PKU82_00035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOZ81290.1"
                     /translation="MMLTNRSKVVVNKEMTFAEKIYIPAIVAGLMITIKHFFKKKATI
                     NFPEQQKPISEVWRGLHVLKRDEQGRENCTACGLCAVACPAEAITMTAAERKKGEEHL
                     YREEKYAAVYEINMLRCIFCGLCEEACPKEAIFLTNRMTPSDTLRDNFIYGKDKLVEG
                     IAPEERVDISYRARAHRKEVE"
     gene            5144..5455
                     /locus_tag="PKU82_00040"
     CDS             5144..5455
                     /locus_tag="PKU82_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81291.1"
                     /translation="MKKLFSGVIITLSLGLMSCDKDVCRDCYLVRSMLCTYTDTTLAA
                     YIIPGDTVKTEQQCDKEKDFKDDKFKEEKYEYDSPSMFYPNYNVHVVVTDIYHCPEKT
                     N"
     gene            5561..6016
                     /locus_tag="PKU82_00045"
     CDS             5561..6016
                     /locus_tag="PKU82_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOZ81292.1"
                     /translation="MSALFTVFSKKPVYSVLYLIFFFFTLFGHYILLNAQFLAAVHLI
                     VYAGAIMVLFLFVIMMLNLNKEIEPNKNIVSKFAAVISSGLLLIVIVSVLKHVDVESS
                     KLNSQTDIGLVESIGHVLYNEYMLPFEMVSILLLTALIGAVMLGKKEVK"
     gene            6018..6326
                     /gene="nuoK"
                     /locus_tag="PKU82_00050"
     CDS             6018..6326
                     /gene="nuoK"
                     /locus_tag="PKU82_00050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOZ81293.1"
                     /translation="MEPVNINHYLLLSAILFCIGVAGVLFRRNAIIIFMCIELMLNSV
                     NLLLTAFSNQHNDASGQVFVIFIMAVAAAEVAVGLAILVMIYRNVKSTDIDVLNKLKW
                     "
     gene            6330..8231
                     /gene="nuoL"
                     /locus_tag="PKU82_00055"
     CDS             6330..8231
                     /gene="nuoL"
                     /locus_tag="PKU82_00055"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOZ81294.1"
                     /translation="MDFLISAIPLVPLIGFLLNGIINKKMPQSLVGIIASGTVLFSFL
                     ISCYLFLQINNGSIKESYYSMFSWINAGSLNLGLSFLVDRLSVIMLLVVTGVGFLIHV
                     YSIGYMKHDEDYSRFMSYLNLFIFFMLLLVMGSNYVVMFAGWEGVGLCSYLLIGFWFK
                     NNDYNKAANKAFIMNRIGDLGFLLGLFLMYQTFSSLEFKDVFAAAGNYPSGDATLTAI
                     ALFLFIGAVGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGVYMVIRSNVIYTLA
                     PFAGEIVAVIGLVTALMAASIGLKQNDIKKVLAYSTVSQLGYMFVALGVGAYTTALFH
                     VVTHAFFKALLFLCAGSVIHAMSGEQDIRRMGGLKKYLPITHFTFLMGVLAISGVPLF
                     SGFFSKDEILAVTWTYSPVMFTILAITSVLTATYMFRLYWLTFYGDFRGTHHQKEHLH
                     ESPSSMTVPLIILAVFSVIGGYIGMPEYINEKLGTHHVFTQFLNPIIYSPKEFILSHT
                     FEFTLLIGTIIGLAIIYVMTKNMYVRDKVVPEDDSQTKSFQKVLANKYYVDELYDMII
                     RKPLEAFGELFYKIFDISIIDGIVNFIPRATNLTSSGLRLLQNGSVGFYLVSMVIGII
                     LIFVFKMLL"
     gene            8232..9689
                     /locus_tag="PKU82_00060"
     CDS             8232..9689
                     /locus_tag="PKU82_00060"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOZ81295.1"
                     /translation="MNNLLLIILFLPLAGMALSLLPSREATGKTAFVFSLISLLLSVC
                     LFIQFDPNGGMQYVINYAWVPDLGINFKIGVDGISLMMILLTNVLTPLIILSSFGRRI
                     ENPKGLYALIQLMQFALVGVFISLDAFLFYIFWELALLPIYFICLLWGGKDRIRITLK
                     FFIYTITGSLLMLVGLIYLYLQTPGSHTFDIQAFYDLTLSSQQQTLVFWLIFVAFAIK
                     IPVFPLHSWQPDTYTDAPTQGTMLLSGIMLKMGLYGVLRWLLPIVPGASADNAFIVML
                     LGIIGVVYASCIAIVQKDFKRMIAYASIAHVGLITAGLFTLKVEALEGAVFQMLSHGI
                     NAVGLFFIADMLMSRLKTHNMSEMGGIANNNSLFAVLFMIVLLGTVALPLTNGFVGEF
                     LLLTGIYQFKAWMAIFGGLTIILGAVYMFRSYQAIMLGSRHLNEGEFHPMTLSEKTVL
                     GTIAFLVILLGVYPSFLMNAAAPSIKSLLMIINAG"
     gene            9715..11091
                     /locus_tag="PKU82_00065"
     CDS             9715..11091
                     /locus_tag="PKU82_00065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOZ81296.1"
                     /translation="MNTIVTLSIVGIAAMLSEVFKLKRIMYTLVIAGLVVALGFSIND
                     WNNFGSYFNQMVMVDSFSIVFSSLMIVTTLLWMIMSRSYFSSDSSMSEHFALIVFALT
                     GGVMMTTFSNMSMLFLGLEILSFALYIMAGSDKTNIKSNEAAMKYFIMGSFATGFLLF
                     GITLIYGATSSFNIQEIANYTQQNKDALPAFFKVGVLMILIGLSFKIAAAPFHFWTPD
                     VYDGAPTLVTAFMASVVKTAAFAMIYRLFVSCFISIYDFWTPVFAVLSAITMLAGNIT
                     AVYQTSLKRMLAYSSVAHAGYMLMALAAPGIASKNALLFYLAAYSLSALGAFNLLYSV
                     HKVTGNEGIKAVKGLFYKHPVSAIMLTICMLSMAGIPPVAGFFGKYYLFTAALKENLL
                     WLVLIAILSSLIGIYFYFKVIIAAFQQPETENTGYCERPSDLAVLGICAVLSLILGIA
                     PQLISGLL"
     gene            complement(11133..13019)
                     /locus_tag="PKU82_00070"
     CDS             complement(11133..13019)
                     /locus_tag="PKU82_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81297.1"
                     /translation="MNIQLQKALLLLMLFFIVFTAYSQDERDSISTHTVKEIIIQGNR
                     LETPFSEAARDIQIITQKEIAALPVQTLTELLVYVSGVDMRQRAPFGTQGDISIDGGS
                     FEQTLVLLNGIKLINAQTAHNVLNLPISLDAIDHIEVLRGAAARVYGINALTGAVNIV
                     TKKSNESFVTANIYGGTSFKRKETGDGDGMYGGGGVDLVGNFANNNHRQLLGLSQNLF
                     NGYRYNSAHKNTKIFYDGDFYINEKNSISTFAGHTYNKFGANGFYAAPGDKNSEEIVE
                     TSIFSLSSKHQLGKVNLMPRISDRYDEDDYRYFKSDLSRGRSKHFTNALMLELNGNLT
                     TSAGVIGMGWESRLTKINSSNIGEHKMDNHGAFIEFRTKLFKRLVTNVGIYVNYNSQF
                     DWKAYPGLDLAYRLNNKWKIAASFGSGQRIPSFTDLYLKQPPANVGNPDVQPETAWQY
                     DANIEFTSGHLKVNVGSFYRKVTDFIDWVRPNMTTPYSPSNFGNIIVQGIYSRINQQL
                     ILSGKHQLNFCISYNFLNPEHYDYKNEQSKYVLESLRHQLIAGVNYRYGSFNVQIENR
                     YIKRELNDAYNIVDGRISYEIHSVLFYANVSNMFDAKYKEAGAVPMPGKWFALGLKYN
                     WKKK"
     gene            complement(13263..14312)
                     /locus_tag="PKU82_00075"
     CDS             complement(13263..14312)
                     /locus_tag="PKU82_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81298.1"
                     /translation="MNKTFTLITTLFCAAAINTNAQLITISATHQMPAIGDTIRYVDA
                     NTFGFNQNGTGPVTNTLWDNALLMNAGTNYSFFYDDPATITGNGVDSFPGATLARGES
                     GAAGYFYYQNTANDINRIGWYSSASNYGVYNTGTYATEFHFPMTAGQTVNSTYSGRYA
                     PFGLGEDSCTLESGSLAINADQQGTLILPTGTFTNVLRIHAIETFHIKIYMLGTPVID
                     YLVSDDYYYWFHDTIKQPLLTYGITTIDGTAQTPVLRYQPVSGTTGLNQLAAHTEMTI
                     TPNPSSGKFLLSNVGSTNATIEVLNALGQKVNFQQTNMGQSLEVDLNNQAEGIYFIKV
                     TDGRTSTTHKVVVGF"
     gene            14501..15727
                     /locus_tag="PKU82_00080"
     CDS             14501..15727
                     /locus_tag="PKU82_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOZ81299.1"
                     /translation="MTEIMSPAGSFESLQAAIQGGADSVYFGIEQLNMRARAANNFTL
                     SDLAEIKKICSEKNVKTYLTLNTVMYDHDIMLMKKIIDAAKKNNIDAVIASDHAALMH
                     AKKIGMPIHISTQANVSNIESVRFFADYADTIVLARELTLNQVADVVKQIKRQKIKGP
                     SGKLIKIEVFAHGALCMAVSGKCYLSLHSHNASANRGACIQNCRREYIVQDKENEVEL
                     EIDNEYIMSSKDLCTIDFIDKLIETGISILKIEGRGRAADYVYTTTKCYKEAVECYHN
                     GSYSATKIEHWKNELATVFNRGFWGGYFLGKTMGEWSNTGGSQATTKKIYVGKGVKYF
                     PKNKVGEFKLESGTLSVDNDIMITGSTTGIILTKVQELRMDDLQVTTVKKGDVFSMPV
                     AEKIKPSDKLYKIVND"
     gene            15720..15947
                     /locus_tag="PKU82_00085"
     CDS             15720..15947
                     /locus_tag="PKU82_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOZ81300.1"
                     /translation="MIEIVQYRSKCIGCNACVEASATRWRMSRKDGKCTLVGGKNNKG
                     IYIAVVNDDEYEQALQAAKNCPVNIIKVKKR"
     gene            15948..16370
                     /locus_tag="PKU82_00090"
     CDS             15948..16370
                     /locus_tag="PKU82_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81301.1"
                     /translation="MDAAIENFISAQKNMTLCTSVNDIPECANCFYVFMQDGAHLIFK
                     SKKTTGHVSHALVNNKVAGTILPDISKIGNIKGIQFSGIFLEPQDVMLEQAKKIYYSK
                     FPLALGISGDLWLIKLTYIKMTDNTLGFGKKLIWENKN"
     gene            complement(16384..19248)
                     /gene="gcvP"
                     /locus_tag="PKU82_00095"
     CDS             complement(16384..19248)
                     /gene="gcvP"
                     /locus_tag="PKU82_00095"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOZ81302.1"
                     /translation="MYYSFADRHIGPDDKQVKHMLATIGVKSVNDLIAKIIPELIRLQ
                     KPLNLPEAMSEFEYLNELKATASENQVFKNFIGMGYYNTIVPGVIKRNIFENPGWYTA
                     YTPYQAEIAQGRMEALLNFQTMVCDLTGMQIANASLLDEGTAAAEAMVMFYYTRSKEA
                     EKKNACKFFVSENCFPQTIDVLRTRSKPLDIELVVGDEQLFSPDDSFFGALLQYPDAI
                     GNVLDYKNIVNMCHRHEVRVCVAADILSLVLLTPPGEWGADCVVGSTQRFGVPMGYGG
                     PHAAYFATHDVYKRNLPGRIIGMSVDAEGNPALRMALQTREQHIRRDKATSNICTAQV
                     LLAVMASMYAVYHGAEGLKAIATTVHKRTTALAESLKKAGFKVVNNSWFDTLTVEVED
                     TESVRDMAEANLINFRYNGNTVGVSIDETTSDEDLKDILRVFAASIGKKSIAAATMNG
                     NAIPAALLRSSEYMTNAVFNTHHSETEMLRYIKRLENKDLALNHSMISLGSCTMKLNA
                     TSEMEPLSWPEFANMHPFAPIEQAQGYAHIISELENFLKEITGFAAVSLQPNSGAQGE
                     YAGLVVIQKYHSDRGQSHRNVVLIPQSAHGTNPASAVLAGLKVVVVKCDDDGNIDLSD
                     LRLKAEQHQENLSCLMVTYPSTHGVYEENIKQITAAIHANGGQVYMDGANMNAQVGLT
                     CPALIGADVCHLNLHKTFAIPHGGGGPGMGPIGVAEHLAPFLPSHTVVPTGGRKGVNA
                     VSAAPWGSALILLISYGYIKMLGKTGVQRATEIAMLNANYIKARLEKHYPVLYKGKNG
                     FCAHEMILDCRAFKAVANVDVTDIAKRLMDYGFHAPTVAFPVAGTLMVEPTESEPLEE
                     IDRFCDAMIAIRHEIMEVINGEYSKEDNMLVNAPHTALEATADEWKHSYSRQKAVFPA
                     PWVAGNKFWPSVKRVDNAYGDRNLICTCPDVSAYETAEAV"
     gene            19621..20289
                     /locus_tag="PKU82_00100"
     CDS             19621..20289
                     /locus_tag="PKU82_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81303.1"
                     /translation="MTLRHFITSLIFLFSATTLSAQDTLLLLNGKTKPVKLFNLNDPD
                     WVRYVKFKESDKKKNEGSARVKRIDVYKVFSIKKADGTEQVLYKPDSTSDDPDIPWVR
                     DFIAGQQYGIMHKRERFNKHDNQWHRRLNFTEVGGIAAGGAGSLLTFYGIPVPAIYAI
                     AVGRHSPALPNDPAIAPEMKNSEGFISGYQKQRRNQRIRQGFISGMIGFAVGIVTFTL
                     IERN"
     gene            20290..20793
                     /locus_tag="PKU82_00105"
     CDS             20290..20793
                     /locus_tag="PKU82_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81304.1"
                     /translation="MFRKIAHKAVSYNLLIALAASAASHLTILRINETNNVVLIAIIF
                     FATLAIYNFPRLPWQKPLNTRTWICLISILFSAILIINYNYISINLFLTAILACLIYM
                     MPFNYKGKRIKGIRNIFLLKNIFLAASWALITVLLPMAALGHESPIIKEVFFTPFHLC
                     IRYFFIV"
     gene            20786..21100
                     /locus_tag="PKU82_00110"
     CDS             20786..21100
                     /locus_tag="PKU82_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81305.1"
                     /translation="MFDIRDTAKDKTRNHLTLPVVLGTTVTRYIVIASAALFILIVSV
                     LYRHEHFTLPMAVAFITSALLLVVATITAPPAANFKFYQVGVDSLLIAQWLLVVVAVK
                     FY"
     gene            21205..22677
                     /locus_tag="PKU82_00115"
     CDS             21205..22677
                     /locus_tag="PKU82_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOZ81306.1"
                     /translation="MSKGIHVKKPLDSLLAEAEDSEKGLKRTLTANSLIALGIGAIIG
                     AGLFVRTAAAAANNAGPAVTLAFVIAGLGCAFAGLCYAEFASMIPIAGSAYTYSYTTM
                     GEFIAWIIGWDLVLEYALGAATVSIAWSEYLNKLLNYLGMHIPYEWCHSPFEHSLDGV
                     YGIMNVPALVILILLSLLLIKGTQESAFVNGIIVVTKVAIVILFIVFGWQFINPANHT
                     PYIPAASTFTDHMGVPHHYGGILGILGAAGVVFFAFIGFDAVSTAAQEAKNPGRDMPK
                     GILGSLIICTVLYILFAHVLTGVASVEDFRTQGREASVTYAIQTYMHGYDWLAKLVTV
                     AILAGFSSVILVMLLGQSRVFYSMSRDGLLPKVFSELHPVYRTPYKSNLILFVFVGLF
                     AAFVPGDIVGDMTSIGTLFAFMLVAAGVIIMRKTNPDAKRTFRTPLVPLVPILGIIVC
                     GAMIFGLGWPNWSRLLMWLAIGFVIYFGYSAKHSKLRNPK"
     gene            22699..24153
                     /locus_tag="PKU82_00120"
     CDS             22699..24153
                     /locus_tag="PKU82_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOZ81307.1"
                     /translation="MHNDKPHLHRSLGLWDATMLVAGSMIGSGIFIVSADMSRHTGSA
                     GWLLFLWLLSGVITLIAALCYGELAGMMPQAGGQFVYIRRAWGNCLAFLYGWTVFMVI
                     QTGVIAAVAVAFAKFTAVFFPYFNTGNILFQAGGISISAAQMLAIFLIAVLTWFNSRG
                     IEGGKTIQNIFTSAKLLALFILIVAGLWVGLKTNTLSTNFGNAWSVNSMIKTDSGWIE
                     QHISGFAIILMLGTAIIGSLFSSDAWNNVTFIAGEIKDPSRNIPRSLMLGTAMVTLLY
                     CLANIAYLALLPLKGSIDAATVAGQGIQFAGGDTDRVGTAAASMIFGDSSVYIMAALI
                     MISTFGCNNGIILSGARVYYAMALENLFFKQAKKLNSKGVPAIALWVQAIWAGLLCLS
                     GTYGDLLDYTVFASLLFYVITISGIFILRKREPDAERPYKVFGYPFLPALYIILALSI
                     CSILLYTKTAVAGKGLLIVLLGLPIYYLFKRRSS"
     gene            24217..26076
                     /locus_tag="PKU82_00125"
     CDS             24217..26076
                     /locus_tag="PKU82_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81308.1"
                     /translation="MRKISTLFAMLCITTAVSAQTQRLVLFEEFTGETCGPCALVNPT
                     LNEMLNDEETKIVSIKYQNNIPSPGPRFWPYAQSDIAARQAYYNNTYSPHGIMDGNQF
                     NDNAGALDPVIINPRYATPSPFEVRVAHTFSAANDTIYASVIIKAAQSYSDSNLVAQI
                     AVTERDIFGYTSPNGETHYEGVLRKMLPSAYGTPLQISWNAGDSVVLNYSWYITPAAL
                     SSPVYYQLSVVAFVQDNSSKEVLQAGFSRAQVPMDPKAKAITGMQDVTCSGTMTPSFT
                     VANNGITDISTLDIQYKVDNNPAQTYSWMGLLPSFTSTTISLPPIPVSGNGSHSITLT
                     TANPNGGVTDNNMLNNSKTAYFGIPGAAVAGNVLEDFTLSTFPPANWIRVDQNGNNTG
                     WLRSTAGNGSAKIDFYSSFTGHIDNLYLYPVDMSGASGATMTFDVSHRQYSAAYIDAI
                     NVQVSTDCGLTWNTEWAKSGAALATVTGYQTGAFTPSAAQWRAETVNLNQYAGNNNVL
                     IRFNAVSGYGNNGYVDNVNVSLSTGINEVANTTFELYPNPSTGIVTIRTKTQNSDYKI
                     RFTDISGKEVFVNTRVEGNTIKADFSACSNGTYFAEIQTPNGTERSKFILLKD"
     gene            26245..27219
                     /locus_tag="PKU82_00130"
     CDS             26245..27219
                     /locus_tag="PKU82_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOZ81309.1"
                     /translation="MSKGPISQFMQHHYRHFNAAAMMDASKAYVTHLAEGGKMMVTLA
                     GAMSTAELGISLAEMIRQDKVHIISCTGANLEEDIMNLVAHNSYKRVPNYRDLTPEQE
                     RELLDNHFNRVTDTCIPEEEAFRRLQKHIHQIWKDADNKGERYFPHEYMYKMLLSGVL
                     KQYYEIDPKNSWMLAAAQKNIPIVVPGWEDSTMGNIFASYVIKNEMKATTMKSGIEYM
                     VWLSDWYRQNSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIDTPKFIVESDASIVAPLMFAWILEW"
     gene            27229..27771
                     /locus_tag="PKU82_00135"
     CDS             27229..27771
                     /locus_tag="PKU82_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ81310.1"
                     /translation="MNAAVKYTLAGIAAVILGFVSLGIIHPEIEYESRVQISAPVTKA
                     FDIFIDTSMMKHWMPGFVSFKPLEGKMLTKGSKQLLVLNLNNEQIEITETVEDYSYPK
                     FYKFHAQNTLLNSTNTIIFEGDSIHCSLTVKTKASGNNFMLRSMFVFTEPIFKQQQDS
                     TYTLLKRTIESHLDNHNINQ"
     gene            27819..29486
                     /locus_tag="PKU82_00140"
     CDS             27819..29486
                     /locus_tag="PKU82_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOZ81311.1"
                     /translation="MNSRILNFLLLLLISTSVNAQSDTELKSNLKQHISILASDKFEG
                     REPGTKGERLSYEYITQQFKNIGLQSLGSNGYLQSFEFSAGTKNGVKNKLTIAGKNLK
                     SDSEYYVLPYSALQATAEGKSIKIGSGLLSKDLGRNDYEGLTVLKGRIAVMDYATPEG
                     NNPHSKWAEASDLRKRIEIAVSKGISGLIIYNSNDSIKNPEKNSEIKITPFAIPVVFI
                     KSNDATLLKDNQQVTIATDIEKIMHSGHNVIGYIDNRAENTIVIGAHYDHLGYGGHES
                     LYRGKPEIHNGADDNASGTSTLIELARKLNSSSLKKYNYLFIAFSGEEKGLLGSNYFV
                     KHPTIDLKKVNYMLNMDMVGRLKPEEHILLINGAGTSPSWDSLLNNIKVDSLRIKTTA
                     SGVGPSDHTSFYLEGIPVLHFFSGTHSDYHKPSDDEPLINYNGMVSIFHFMYDLIEQT
                     NNLPKLTFTKTNDSSNEDAPRFKVTLGVVPDYAFEGSGMRIDGVTEGKPAAIAGFLKG
                     DVVIQMGEEKVADMMSYMKALSKFKKGDTVKVKVQRGKDVVEKEVTF"
     gene            29503..29988
                     /locus_tag="PKU82_00145"
     CDS             29503..29988
                     /locus_tag="PKU82_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81312.1"
                     /translation="MTQRTIHFEEKQYLGLNHYSLLRSMVLALFCFVAYYVTENRENN
                     AELLFFGGVAFMVISLLSMFIKHLHTRVYDSCIELDGLWTMRKVKIDLSTIVDVQKIP
                     YSNYIMNTPVYNLHRNGSIYFYTSGRSAIKITDKDGMNYIIGTQKQEEFLRILNDLIK
                     K"
     gene            complement(30034..30312)
                     /locus_tag="PKU82_00150"
     CDS             complement(30034..30312)
                     /locus_tag="PKU82_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81313.1"
                     /translation="MKKTVLTLAVVAATYCANAQTEKKAEDQAQPVKSEQATTPQKEV
                     KKDAPATIQAAPANHESAPADKEVKKDAPQKDSHKATEMEKPKDKPKE"
     gene            complement(30370..31635)
                     /locus_tag="PKU82_00155"
     CDS             complement(30370..31635)
                     /locus_tag="PKU82_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ81314.1"
                     /translation="MNRIRIFLKLSAESIAFALHALRANMLRTVLSLLGISIGIFAII
                     SVFTVVDAMEKNVRTSVESLGDKTIYVQKWPWAFGGDYPWWKYWQRPLPSVKEMHQLA
                     ARSQSIEAFTFEAYLNGKTVKYLNNSAENVTVALVSHDFDKVKSFELSAGRYLTEMES
                     NSGRAVGIIGSNVANGLFGSASNALGKVFEVMGRKVEVIGVFKEEGKSLLETEMDNTV
                     VLPLNFGRNFVNIRSDRIDPFIKAKVKEGVPIQFANDELKSIMRSIRRLRPIEDDNFA
                     LNQSSLLSNNITNIFSALNMAGAIIGGFSILVGGFGIANIMFVSVKERTSQIGIQKSL
                     GAKNYFILMQFLFEAIVLCLIGGLIGLSIIYFLAMFVSHAFDYPLSLTGHNIMLGLFI
                     SALIGLVSGFIPAYSASQLDPVEAIRANG"
     gene            31755..33284
                     /gene="purH"
                     /locus_tag="PKU82_00160"
     CDS             31755..33284
                     /gene="purH"
                     /locus_tag="PKU82_00160"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOZ81315.1"
                     /translation="MKIKSALISVYNKNGFEPLIKALHQQQVTLFATGGTFDFIQSLK
                     IPITAVEDVTDYPSIFGGRVKTLHPKIFGGILNRRNDANDVMEVEKYNIPCIDLVVVD
                     LYPFEATVASGADESAIIEKIDIGGVSLIRAAAKNFNDVSIIASADDVTAFLQTYNQG
                     NGEVNLSFRKKLAQKAFAITSHYDSAIFNYFYSKNEQSEPVLNLQCNNSVTLRYGENP
                     HQRGYYFGNLNETFVQLGGKELSYNNMLDCDAAINLIAEFSNEKGVTAAIIKHNNACG
                     IATREKATDAWQAALAGDPVSAFGGIIVFNQHIDESLAKEINAIFFEVIIAPSFAEEA
                     VKVFSEKKNRIILQLKKQLVAKQSIKSALGGYLMQETDSEALSTDGWNNITLRKPTTT
                     ETEDMLFAVRVIRNLKSNAITLVKNKQLIGSGAGQTSRVDALKQAIAKAVNFGFNLKN
                     SVMASDAFFPFPDCVEIAKTAGIEAVVQPGGSIKDKDSIEYCNKNNMTMVLTGKRHFK
                     H"
     gene            33407..34429
                     /locus_tag="PKU82_00165"
     CDS             33407..34429
                     /locus_tag="PKU82_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOZ81316.1"
                     /translation="MGLFDFLTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRMTGK
                     VLAVGKQAMMMHGKTHENIKTIRPLKDGVIADFDAAENMIRGMIKMINPGRRFFQPSL
                     KMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDGFTMDIWDYMRRQHNILIGERSAEMIKIEVGS
                     ALPELDNPPHDYAVHGRDLMTGIPKEITVTYSEIAHALDKSISKIEEAILKALEMTPP
                     ELSADIYRTGIYLTGGGALLRGLDKRIGMKTKLPVHVAEDPLRAVVRGTGIALKNIDR
                     YKFLIP"
     gene            34791..34970
                     /locus_tag="PKU82_00170"
     CDS             34791..34970
                     /locus_tag="PKU82_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81317.1"
                     /translation="MEEQENKKKASGLQVVMDDVMLLLFLGVTIYAIFYIIWGIMEIA
                     TVPNIPESVKQSILK"
     gene            34991..35920
                     /locus_tag="PKU82_00175"
     CDS             34991..35920
                     /locus_tag="PKU82_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ81318.1"
                     /translation="MTLIAPTEGWFKKKVSKDERMWIIISFVVCIFLFAWMVLWHVFG
                     RQNPSNTTYKTNPIEFAQLHDEYVKRNMVGMDNGIPIVKPEPGSDVILMAQMWRWSPV
                     LILKKDEWYNIHISSKDLVHGFSLQPSNMNFQVYPDYDYVLKFKPTVAGEAKVLCNEF
                     CGIGHHTMIGKILVIENDADLAKYGYNNLSAPPARTGKGTAEEDISGWSEEQLIKGGE
                     MVYNLKGCMACHKTDGTSEIGPAFNSIYGSKVKVKEDGNAKEIVIDDDYITTSIREPQ
                     KQIVVGFEKTPMAQMNLTDNEIKQVISYIKSIK"
     gene            35933..37600
                     /locus_tag="PKU82_00180"
     CDS             35933..37600
                     /locus_tag="PKU82_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ81319.1"
                     /translation="MFRICDITGFKVDLQSEKFIRLNAVMAVVSLLLAITAAILLVFT
                     RYQPVHLLDAEWYYRLTTFHGLNALIYWIVFFEIAGLYFGSTVVLNQKFCSPTLGWVT
                     FLLMVGGMVLSNVMVLMGKADVMLTSYVPLKAHPLYYLGIILFAVGALIGVGLFFANI
                     VNAKRDGNVGETLPLFTYGLMTAAILAVITLASGAIIYIPTFLWSLGLIDSVDPAVYK
                     LIWWGLGHSSQQINVAAMVSIWYLVTFLALGGTSINERVSRTAFFLYILFISLASAHH
                     LLTDPATSSAWKVWNTSYAMYLAVLASMIHAFAVPSSIESAQRRWGFNKGLFEWLSKA
                     PWSNPAFAACVLACIGFGFIGGTTGVIFGMEQTNIIVHNTIAIPGHFKGTVVIGTTLT
                     FMGITYYLIPLIFRRKIVAFKLAQLQPWMFFFGVAILSVSMIALGQLGVPRRHWDSTF
                     SGGPFTHEFNPSVDFFWTLMMLGGTLAFLAALIWVLQVVVSVFFGPKVNGPEDMQLPL
                     SPMAPAAKEHKGYEAPGTLILTLLFLATFAVLFFVNWKWLAATWWVK"
     gene            37631..38407
                     /locus_tag="PKU82_00185"
     CDS             37631..38407
                     /locus_tag="PKU82_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ81320.1"
                     /translation="MINNKMQLTGLVILLSLFFSLSSFDIPDEKKFIGREVKNIPILD
                     SKGNNLQLFDYPTGKPIVLSPVYTRCPMACSLISNGLKTSIEKLGTLGKDFTVISFSF
                     DSTDTKEDLSGFEARWKMDGVNWKTVTSNAAGIGELLRSIDYHYEYDEKSKEYLHPNL
                     VVVITPSGRISRYIYGVEPKAIDLELAIMEAQVEKTSFNNFFKTLYIRCFTFNPATKS
                     YHLDWKFISQTSVGMMFIIIVGYFLIKSLYSNNTEDSAEL"
     gene            38404..40326
                     /locus_tag="PKU82_00190"
     CDS             38404..40326
                     /locus_tag="PKU82_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOZ81321.1"
                     /translation="MINNKSSHWLFQRFAGIFSRIYSSKLNPLYHLGDIAILMFIVAC
                     ISGVYLFLFYNIDPAHAYESVEKISANPFNAVIRSIHRYSSDLLVIFIVLHFLQTLLT
                     GKFKRILSWVSGIISLMVVLIIGVTGFILVWDEKGKLVGYLTAKFFSAIPLFDPSIAG
                     SFISNNLDVIGGFFKVSVFGHIFFSIFLVIVLWLHVMRIAKPKLFSPRIIWIYSMIAL
                     VLVCLVFPVKSDPSAQERFIPYNTTFDWYYFFGYSFMKVVSVPMNWVIMLVSGAGLAL
                     VPLIFKKKNKPATIDLDKCDACNICSEDCPYGAIDMLIYKGERKAILDPDKCISCGIC
                     LASCKEHAITMPGIPEHNVLPLNLAQKSQLSIVSCSQFDDVKIPDGITHKKKSLPCLG
                     DLHMHEVEQMLNEESEGVLLLGCEDCYYRYGRNLAVARINRKRPPGLAKKLSRDRIQL
                     ITTNHYSAKAIREFTDSLASAKPNDGKATIKVLDYFKTNHIAAALILCAFFLLMPFFS
                     NTKLSFFDKDEKLLVLNFKYISSPTEFQEQNTNQQRQMQSLKPIVKRRSPIMVEVAGS
                     DGNILLKKEFSPRGLRKDISIFVYSEIPTTDSLVNIKVTETAFPDKVVSLQNVALSTI
                     DGTIISFADGKLKQLK"
     gene            40438..41532
                     /locus_tag="PKU82_00195"
     CDS             40438..41532
                     /locus_tag="PKU82_00195"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HOZ81322.1"
                     /translation="MWRNLTTTFIISLFTVNSLTASKLQPGYNPKEYMELLSFQLMQF
                     HKQDSLYPDSMHYHNFKEVYKSPEMGLLNQWSLWMGADSVAVISIRGTIANSTSWLAN
                     FYAGMIPAKGQLILAPGDTFNYHFADDSKAAVHTGWTLATAYLIRDILPHIDSCYKKG
                     ITNFIVSGHSQGGAIAFLVTSCLYDYQKNNQIASDIRFKTYCSAAPKPGNLYFAYEYE
                     NMTRNGWAFNVVNSADWVPETPITIQTTKDINNTNPLVNAKAGIRKQKFPANLFVMHI
                     FNRLQKTPYKAMRQYRKYLGGFAGKLVRKNLVNFQSPIYLNSSDYVRTGTTIVLLADK
                     NYFDLFPDSKTKIFVHHFYEPYLYLAAKRQ"
     gene            complement(41630..48811)
                     /locus_tag="PKU82_00200"
     CDS             complement(41630..48811)
                     /locus_tag="PKU82_00200"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ81323.1"
                     /translation="MSAMLWTTSAQAQLAIKYPLTISSGTYSSISGSGAPVAIIGDDM
                     AVNITGLTGFTVNGTTYTNARMCSNGWLALYGASVPTTTGTYAPLSTAITNGAVIFAP
                     FGRDLNSQSGTGAYWQTIGNELIFEWVNYIRYAQTGESLNFQIRLNTVTGEIKFVYGT
                     MTALTGSTYPQVGWKTNGTVASNWATDVNNLMIDNVGSPNTCNWSNAVTGNANTSTMY
                     FNSANATIKPNSGLTYTWTKPVATDPEPVRTHAAVSGITTNNATLNWTAPANATQYNV
                     QYRTVGSCSWTNWSGNPVGTNSVTLTGLTSSTTYQVRVQASNGSTNAIYSRIPNLAGT
                     GNGYVAAGTFTTVASCVAPTAPVGSPTTATTANISWTAASPVPSNGYEYAVTTSAVPP
                     VSGTAFAGTSTSVSGLTPATTYYLHVRSYCGGSDYSAWVTSASFNTPCNAIATLPWTE
                     GFESAVIPAYPSTCWYEDPSSGDWITTNNSSSSNDADARTGAQFLRDAYSATNEFIWT
                     PGFSLTAGQSVDFSFWWAGDNYSGWTGDVFYNTAQTGTGATQMGTSFVTSGTTTTKTY
                     AKQTRTLVAPATGTYYFAIRVNATGGPWYLSFDDFNVENTPSCVAPTALVGTPTTATT
                     ANISWTAASPVPSNGYEYAVTTSATPPGSGTAFAGTSTSVSGLTPSTTYYLHVRSYCG
                     GSSYSTWASSASFMTGQIPATLPYSNSFGSNDFAFVNGSQTNQWAYGSATGNPANAIY
                     VSNDNGTSNAYTISSLSVVQAFRDIAIPNGTTLTNFSFNWIANGESCCDYLRVWLVPT
                     SYNPTAGTQITAGAGRIQIGSNMNVQSAWQTYTNNTLDLSSFAGQTMRLVFEWKNDGS
                     IGTQPPAAIDNISLAQVASCSGTPAPGNTQSNLPSACSAQSFTLSMSNTINDIGITYD
                     WQSSPDGITWTSTGGTNATYTTTQTAATYYQCVVTCTNSGMSATSTPVQVTMSPFYNC
                     YCTTNLHTSSSPCITQVDFNTISNNTAGAGCALPAYSYQAATTTVLKGSSYTFTRTTT
                     STGVWTGVWIDYDQSGTFDASEYTEVSSTSTGALTNSVTITIPVTALDGNTGMRIRQR
                     TVDMLATDACTQGFGSGETEDYTVDIQTPVGCSGTPSATIGGAPANVCAGVPFTLTAG
                     PTGLAGFTYQFQNSTDGGLTWNNMGIADVFNNYIVSNQTVTTMYQVIVTCTNSGLSNT
                     STAVTVTQNSPTACYCTPTTVNGFTYYITNFTTTGGITNINNSSVGGGPYQNFSGSAS
                     ASANPGATINYSISVTSGTYGRAIWIDLNEDGVFQATEQLVTSSSFLSSPLTGSFIIP
                     NGTTAGTKRMRVLASWSYSGPTDPCNISGNGEYEDYSFEVVALPSCTGTPAATISGPA
                     NICAGIGFNLTAGPTGVDGLTYQFQNSTDGGITWNNLGSAGINNIYAITSQSVTTQYQ
                     VIVYCSYSGLNATSTPITVTQNLPSACYCTPTTGGGLTYYISNFTTTGGTTNINQSSG
                     GSSTGYDDHSATVSGEFEPGSTINFTMTIAGSSNFGHAIWIDFNADGTFQATEQIVTS
                     NSYLYSPITGSFTIPLNATTGVRRMRILTSFTPSNPTNPCTNSGQGEYEDYSINITPP
                     VCISAPSSPANAGSACADVITNTVLLSWPSLSGATGYDVYFDANNPPTTLVSANQGGL
                     SFSADVSGGAPVYYWMVVPQILGGGSSCTVWSFSLSPSPTPIASSGGDVCLGNDISLT
                     GDNIAMGQSSGNTYSWTGPNGFNSTLQNPTIINPGVLYSGTYTVVVTNQYGCSASEST
                     SLSVNDNPTLSIDSLKNVGCIGGSDGIIYVSASGGLSPYSFTSDFINFNVDGIMNNLP
                     TGMSTIYVADGNACQAQISGSLTYTSTAPPAQSVNVPILGMPAYACNGTTATISVAAV
                     ANATQYTWDGPVGTTFNGNPSPYISTTPSVTVVFGAPNGSGYRIGVQAGNGCGSSLRK
                     SQWLRGIVSTPAAVSGATTTCANSSGTYSTVAVDGATSYLWTVSGDATVSGNGTSVTV
                     NFGPSWTGGTLCVSAQTPCFNSPSKCLVIGSSASPLNVISGSFTACPNQTLTFSVPAS
                     TGAASYNWTLPAGATGSSTTNSINATFSPSYNSVGNICVSVTSICGVTSPIKCKTVAP
                     GLPSVPPAITGITNGVCGQSVSYSVAPVSGITYNWTAPGTVIGNGNSTVSVQYGSLTT
                     GQVCVTASNSCGTSASRCVSVKGTPNSPIALTAVPSSWCANTQGIEFTADMSNTTGSY
                     TISWTYPSAPTASYVAGGGNSNALTLDWGTGNGSVVVTATNSCGAGSKAFAVTIGCKE
                     GEMASASKLNVYPNPTAGVLNVEYTADKGTAQVTVLDLSGRVVMTQSHANAAGQNILQ
                     LDLSKVAKGAYMLNVQTEGSNNQVRVVVE"
     gene            complement(49194..50183)
                     /locus_tag="PKU82_00205"
     CDS             complement(49194..50183)
                     /locus_tag="PKU82_00205"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOZ81324.1"
                     /translation="MLIVRLFKSSQTPPLFLVPVITLALVLFNIYTQGVVVIRDMNHG
                     VLYALLLDSNIAGIKWAVPSLIFVLITIQAFLFNALINRFEVLYKSSNIPVIIFIILY
                     GLIPGFLALSPFLLVNTLMPVALWKIFTLYKSERPVTRAFDTGLVFSLMILCYLPMAL
                     LVVFMYLCIAILRPFNWREWVASALGLLTPAILLFTILFVADKLHLRNTEVNTDSWLP
                     VIRLQSLSWRFILPALVMAILLFLSFLKLYANYSKNVIRMRKFQLCFMLLLIFLLLII
                     LIPVQQSASRFILLIPPVCLFLSYYFIAIRKVWWFELLSIVLVGLIVMGYWNL"
     gene            50255..50458
                     /locus_tag="PKU82_00210"
     CDS             50255..50458
                     /locus_tag="PKU82_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81325.1"
                     /translation="MLNSIWHGIAAFFEFIFSLAKPIGKSVDIVFMVLIAIGLLYWLI
                     YEQRVMGGKTRNYLADKVKEKNK"
     gene            50606..51493
                     /gene="cyoE"
                     /locus_tag="PKU82_00215"
     CDS             50606..51493
                     /gene="cyoE"
                     /locus_tag="PKU82_00215"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOZ81326.1"
                     /translation="MLKTTMVVAPRTFTARLVDYGQLFKLRLASLVVLSAALAFLIGS
                     TTIDYTKLWLLVLGGFLVTGSSNALNQIYERRTDKIMKRTRMRPLPDSRMSLTESLVV
                     TIAAGLLGLFILTYYINLYSGILGLIALVSYAFAYTPLKGVTPLSVFVGAFPGAIPTL
                     LGWVAATGQFSYQGWILFSIQFMWQFPHFWAIAWKLYDDYMSAGFQLLPSGGKNSASA
                     FQTTIYTFTLIPIGLLPYYSGMSGIVSAVVITLAGILFFMQSVYLLKTCSDIAAKRLM
                     FGSFIYLPVVLIAILLDKI"
     gene            51499..52074
                     /locus_tag="PKU82_00220"
     CDS             51499..52074
                     /locus_tag="PKU82_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOZ81327.1"
                     /translation="MQTVTTEQDNNYTGIPTAKILLWIAMASMVMLFAGLTSAYIVRK
                     NEGNWIEFSLPKMFTISTITIIISSFIIQYAIYAIKRNLLNQVKIAVITTLGLGIAFV
                     FMQFYAWTVMVNNGIHLVGNPSGSFIYVLSGMHILHLAGGIISMIVISVKSILERYSP
                     KNYLGLQLGAIFWHFLDILWVYLFVFVTFIA"
     gene            52208..53023
                     /locus_tag="PKU82_00225"
     CDS             52208..53023
                     /locus_tag="PKU82_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOZ81328.1"
                     /translation="MSTATEAKSKSIWGGGISPFNVSWGKMFMWFFLVSDAFTFSTLL
                     VAYGTMRHRFTDIWPAPGDVFTHFPFIHEHVPLAYVGLMTFILIMSSVTMVLAVDAGH
                     QRNHKSVAMWMGLTIVGGLMFLGSQAWEWYHFIHGSKEGTYILADGRFARTDDYEPGK
                     LFFWHNHQKTVVEGAEAEKLKASAVRNIKGANMTENEYGNTPLFANFFFFITGFHGFH
                     VFSGVVINIIIFINVLNGVYKRRGHYEQVEKSGLYWHFVDLVWVFVFTFFYLV"
     gene            53048..53413
                     /locus_tag="PKU82_00230"
     CDS             53048..53413
                     /locus_tag="PKU82_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOZ81329.1"
                     /translation="MSHNEHNEHKPHGYYTGDIHNQFVEEHYDPTAKKIIWRTFWILL
                     VATIVEVAIAFIPMHKDLLQWIFVALTIFKSAYIVGVFMHLGKEKKSLQYTILLPFFL
                     IVYMIAICLIEGVYLFHIG"
     gene            53467..54060
                     /locus_tag="PKU82_00235"
     CDS             53467..54060
                     /locus_tag="PKU82_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ81330.1"
                     /translation="MPLFFFILMKEGKHNFRYLEYYGPKHLATNGVDTIYHSISDFSL
                     KNTEGNTVSPSNTEPSYWVFQFFNASCQEPCKEVFTNMYDVARDFVTAPKVKFVSITT
                     QPFNDSLPVMKHVMKMNSIHPPQWQLCTGDSLGIDSLMRTSFFLKGNTNAALNSVFLV
                     DSKLHLRGIYDGADLLEMKRLKDDIKILSYETRQKSK"
     gene            54062..54613
                     /locus_tag="PKU82_00240"
     CDS             54062..54613
                     /locus_tag="PKU82_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HOZ81331.1"
                     /translation="MKQILMNEKLTFRVVAIISVVVLGMVMLLSQKVLPRPETPPAFT
                     KYLPGLNAFVNGTCFVLLLISFYFIKKKRIDIHKKINLLTFLLSAVFIVSYFLYHYLA
                     EETKYPVDHPLRGLYLFILASHIILAAIVMPLVLLSFYYGLKMDVRKHRKLTRFSFPI
                     WLYVTLTGVIVYLMISPYYSFNQ"
     gene            complement(54624..57155)
                     /locus_tag="PKU82_00245"
     CDS             complement(54624..57155)
                     /locus_tag="PKU82_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ81332.1"
                     /translation="MSNEISQKISHLREQAEALMTVNTAKAIILLSEAMNHIDADDEK
                     LKSECGLQLALAYHKVKEELMAIKIINQCLELKVNSDSLKNQIPFNELAAEIYTAISI
                     YDSALEHLLNIASAYTSAKDKQKLGLVLNQIGETHKLLGGYNEAIRHHEKALKIFEEL
                     DNKEQLSITNFYIGNCYNWADELDIAFNYLDTSLKIAEKLRSPELKIKTLGSLAILFT
                     KQNKFEKSLDYFFQAIDNSNLAGNVRVKADLLKSLGNLYNQMHRHDEAIKILLEADEI
                     CQSLNIKQPLHLVNLFLSDAYAALNNFEKSLAHHKLYYKLCREIQSEDVSHRTKSLQL
                     KYDLEEIQREKSKAEESLLLKDTFLANVSHELRTPLNGIIGMADLLTETVTTDENRKY
                     VQTILSSAFQLSNIVDEILDYARLNTGQVSPVEHPFILNDTIKECLDKFLLKFPGKKV
                     ESDTDFAACDEVLLDEGILKKVVFNTLVFGGSFASNDKLELALYHSQNKIFINVKFNI
                     VNKKSFPHPDNFFELNNPQFSQNGLNGYGLGLSLINARGMIVACGGKISVKLESSKCN
                     FVIELPYKAVKSDRKNIVSEPPAATEDVTILLVEDNKINQFLAKTMLLKKGYKVELAA
                     DGDEALTLLRKGNFSMVITDVQMPGMDGYQLAGHIRSKLPDPINTIPILALTAYESSA
                     EKEKAKAAGMSGFLTKPYMPEQLFDLITQHLPLKNDQSKDAVEYESTYNVNEIYDNLL
                     ALMAGDKKETASLIKMLQVQFPELLSEMLQAIEDKDMKKLYKAAHKIKSSLKIFNNNS
                     VGLLIDQVERKAKDNEYSFELSVKVSELIKQVNVIIGSLDQLIKK"
     gene            complement(57226..59187)
                     /locus_tag="PKU82_00250"
     CDS             complement(57226..59187)
                     /locus_tag="PKU82_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81333.1"
                     /translation="MYLLFAVQLLQAQSPTAYERAGDKAMKEGDYYSAFAFYFNSYAS
                     NNTDLVITWKLAEACRLYNDYENAQKYYNKAYGLDKQGQFPLIAFYLAEMNQRQGYYE
                     EAASIYNNYLQMHGRDNSYFTDKAKRELANCQNIKQLLKDTAKVTIQNSGTEINSVYS
                     DFAPSYLDSTTLLYSSLRFENKDKKTQKKNKYISKLLVSNNERNTWSRPDNYSTQLNR
                     PGLHSCNSAVSGNGKLLVYTQCQRERDKLICTLYYSTQKDGNWSDPKPLNDSINFKGY
                     TATMPAIGNDELTGYVLYFVSDRPGGLGKADIWKCSITPDFVFGNPVNVGAPVNTVDD
                     DITPFYDNLNSTLYFASDGHKGLGGFDIYSYKPDSQEQTIQNLHYPINTSYNDLYYSS
                     RNNNFLLSSNRPGSLYIKARTCCYDIYDGVYLDTVKQVKKDTLIAIEPIASDITKDSI
                     TIEVIKQIEPLLPMKLYFENDQPNPKTMLTTTKSNYVDLYNAYLTNEKKYRENYVDVL
                     TDESQRNKAAAEITTFFNQVVKKNYLDLDRFSDLVLKQLDAGNKIEIIVRGSASPLAE
                     SGYNKNLSKRRISSVINFWRVYDSGKLNNYIKAGKLTITEDAVGEEMTTTKISDDLKD
                     KRNSVYSPAAGSMRYIQVMRVMINGVAVE"
     gene            complement(59238..60248)
                     /locus_tag="PKU82_00255"
     CDS             complement(59238..60248)
                     /locus_tag="PKU82_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOZ81334.1"
                     /translation="MKNLLSGIFTICLFCLNAAGQDVHFSQFDFSPLHQSAAQTGNFK
                     GDYRLTAINRVQYKSVTVPYKSLGASVDMNIAKTDEKKSWWSAGLLFENDVTGDADYK
                     TTIVDVSVAWNSFLDENKKHQLTLAIQPGWMQNGLNFSELYFGSQYDGDIFNPNLPTG
                     ESPARSSYSNFNLNAGAGYKYAFSENSDLNLIFGASHLNQPDISFLNDKAKLFTRLTG
                     VGVLNLMLSEKISLQPQVLFYRQGKFSQLNGGANVQLHSLENNFKNFRFSAGVFYRNK
                     DAVIARIGAGIGNLDAGFAYDFNTSDLKRASNGRGAYEVALSYIITKVKPLKKNPPCP
                     VY"
     gene            complement(60252..62630)
                     /locus_tag="PKU82_00260"
     CDS             complement(60252..62630)
                     /locus_tag="PKU82_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ81335.1"
                     /translation="MKSHFWKFCLLINFLFLFADSAKATHIVGGEIFYSYLGNGNYQV
                     TMYIYRDCANGVPPFDDPAAIGIYDQSFNLEQALFVDVRPDSLLIPSTINNPCFIPPV
                     NICYRRATYIFNVNLPPSSGVYYIAYQRCCRNNTINNLIGPGVTGATYVGEIRASSFF
                     NNSSPRFKFLPPPFVCLNFPFVFDHSATDPDNDTIRYSLCTPLVGGDTIVPAPLPPVS
                     APPYNNVIFAPPYSVNNMLNGTPGIQPLSIDSITGVLTATPNTIGQFVIGVCAKEYRN
                     NVYLGQTRRDYQLNVVPCPSLVVAAFLNPIVSCGSNTVSFQNGSIGAISYHWDFGVPN
                     LTNDTSNAFNPVYTYPDTGTYNVTLIAYSLFNPGCADTIHGVVTVTKELITDFTSTVQ
                     ACTTVALFNDTILSSAPASFSYNWNFGNGTTSNVSNPQVTYGSPGNYPVTLVVTSSLG
                     CKDTVTKTINIQQFMSATATTIQNVRCKGECNGSGLVAVTNNNGPYTILWSNPPGQSN
                     DTIVNLCQGNYSGTVTDSAGCTSNFSLNISEPPQLTLSATATPDYCSGQCIGTATALA
                     GGGTPPYNFVWSNGSNSGSLNQLCAGNYTVSVTDSKGCTTSPVTVSVLFSDSLAALDV
                     VPSIDTIYRGQSVNLSATQNTGYIYNWQPPTYLNSNGIANPVSTPPVDIEYILSITDA
                     FGCKINDTAKIVVIQYRCEEPEIYIPNSFSPNGDRVNDEIYVYGGQIKELLFRIYDRW
                     GEKVFETKQPGQGWDGFYRGKRVTPGVFVYYVEATCYDNQKFFKKGNITVIR"
     gene            complement(62773..64581)
                     /locus_tag="PKU82_00265"
     CDS             complement(62773..64581)
                     /locus_tag="PKU82_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81336.1"
                     /translation="MNRSIVSFLILFWFCGSAIAQTDTTKNKSVDFYQGVEQVSKSSK
                     LLRSIYKMIFRPVTAKAVKVKKKPVVQEINFSEFQCLPVGNISITVFDPFGYSLADSS
                     IKKQPSVINAGNYLHNKTSNRQIRKLMIIETGDEFDSLLVSESERLIRAQPYIRDAII
                     IPHKNELNQTIDLEVRVLDNWSILIGGGMSETNTKISVTERNFAGLGNTFENSYRWYY
                     NKNADIIESSYEVPNLLGTHADAKILYHLDEREGDVKSIGVERPFYSPLTKYAGGILL
                     EQHFVKAVYGEPSENYSERYKYNFIDAWSGYSMRLLSGNKVYQRNTRLIAALRGSSVQ
                     YLSFPSAGFDSLGLLKNQNTYLAGIGFSSREYITERYVFKYGQKEDIPVGRTYGLTVG
                     YRERASSGNWYAGAMAAFGDYFSFGYLSSLVRYGTFFYNGNARNSAFEIQTVYFTRQM
                     AIGKWRLRQFLRANATLGIDRITNEFVSLEDQMRGFDSRTSGTKRIFISLQTQTYAPY
                     DFLGFKFGPYLIASAGFIGNQLHGFNRSRMYSVFGVGMIIKNEYLALSSFQISLAYYP
                     TIPYSGQDVFRVNPLRSTDFQLVDFDLKKPDIIEFK"
     gene            complement(64667..65860)
                     /locus_tag="PKU82_00270"
     CDS             complement(64667..65860)
                     /locus_tag="PKU82_00270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOZ81337.1"
                     /translation="MRKLADRIERLHESQTIAMARLSRELQDQGHNIISLSLGEPDFD
                     TPKYIGDAAKKAIDDGFTKYPPISGYADVREAISQKLKRENNLDYKASQIVVSTGAKQ
                     CIANAVLCCVNPGDEVLVPTPYWVSYSQIIQLAGGVPIYLNTTIDSDFKVTPQQVEKA
                     ITSKTRMFIFSSPCNPTGTVYSHDELKALAAVFAKHENIFVLSDEIYEYINFVGKHHS
                     IASFEELKNQVIIINGVSKGYAMTGWRIGYMAAPQDVATACDKMQGQFTSAASSIAQK
                     AAKAAISISTPDRDMMVETFRKRRDLVLNLMKDIPGWKTNTPEGAFYVFPDISYYFGK
                     TDGSTVINNPEDMCMYLLKDAKVALVTGEAFGDNQCIRFSYATSDKLLTEAIARIKVS
                     LAKLK"
     gene            complement(65967..67154)
                     /gene="kbl"
                     /locus_tag="PKU82_00275"
     CDS             complement(65967..67154)
                     /gene="kbl"
                     /locus_tag="PKU82_00275"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOZ81338.1"
                     /translation="MYNTLKPVLEKELEEIKSAGLFKQERIISTPQAADITANGKEVI
                     NFCANNYLGLSSHPKVVEAAHAAINSHGYGMSSVRFICGTQDIHKTLESKIASFLETE
                     DTILYAAAFDANGGVFEPLLNEQDAIISDELNHASIIDGVRLCKADRHRYKHNDMADL
                     EEKLKATMNKRHRMIVTDGVFSMDGTIAQLDKICDLADRYQALIMIDECHASGFMGKT
                     GRGTHEHHGVMGRIDIITGTLGKALGGAMGGFTSGRKEIIEMLRQRSRPYLFSNSLAP
                     SIVGASIAVLDMLSQTTTLRDKLWDNTQYFRKQISEAGFDIKPGQHPIVPIMLYEAQL
                     AQQFAAMLLDEGIYVTGFFYPVVAKGNARIRVQLSAGHERHHIDKALNAFIKVGKKLG
                     VIK"
     gene            67248..68006
                     /locus_tag="PKU82_00280"
     CDS             67248..68006
                     /locus_tag="PKU82_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ81339.1"
                     /translation="MRIVRIVFGNVWRFWFFFEAVVTFFLLFPFLLLALQGKAPYRQA
                     FKLMKFHARTIAFLSGINVKVIRKYEPMENETYIICPNHISYLDIVLTYVAFPHYFHF
                     MGKAELERVPFFNIFFKRMNIGVNRSSRMASHKAYQRACKDLSQNISISIFPEATIPD
                     CNPQLGPMKNGAFKLAIENQIMLLPVCYIDNWKILPDCPRKILGGRPGIARVIIDKPI
                     EVKGLTEADVPELKKRYRERMDNILQEYQYGYGK"
     gene            68065..68811
                     /locus_tag="PKU82_00285"
     CDS             68065..68811
                     /locus_tag="PKU82_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015273866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOZ81340.1"
                     /translation="MIKIISISILSMLLFAITQPNDFLAEQKKFIRVRTAIEEKQSFI
                     EEKLKEHKITSDNLNILIVAYKEDDLLDVYAKTIQETTYKKILSYNICSRSGQPGPKR
                     KQGDGQVPEGFYHINRFNPTSSFYLSLGINYPNLADKRKSNSSNLGGDIFIHGSCVTI
                     GCLPMTDNYIKEIYLISVLAKNCGQSKIPVYIFPFKMSDQNLTTYCAKYKENKGLISF
                     WNNLKTGYDKFIKTQKELAIGVNENGDYIY"
     gene            complement(68930..69379)
                     /locus_tag="PKU82_00290"
     CDS             complement(68930..69379)
                     /locus_tag="PKU82_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81341.1"
                     /translation="MKLFNYVMTAALVSAVLFTSCKKKDDDNASPNPPAQSAGFYYGE
                     NGTSTLTKADSSWVNGAFNTIIAQTGGSTVVEINLTGITAQGYSLGTTNSLTYIKNGS
                     FWATTAGTVTITKNQDSKLSGTFSATAGGGVSGVTSFSGNFTDIPIK"
     gene            69638..>70290
                     /locus_tag="PKU82_00295"
     CDS             69638..>70290
                     /locus_tag="PKU82_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ81342.1"
                     /translation="MKKLVFWFLLLSTYSTFAQKISVKGNVQDTTAREPLSNALIMAI
                     KLMDSTLVNYTRSDEKGFFNINALPVDTYQVIITHPKFADMGFFIFGDSTNLEYDFGK
                     IILQPKNIDLHEVTIYAFKDPVYYKGDTLIYTADSFKTKANANVEDLLKKLPGLSVDA
                     SGKITSQGKSIDKVLVDGDEFFGSDPTMATKNLAASSIESVQVYEKKSDDAANSSTG"
BASE COUNT        21811 a        13107 c        13120 g        22252 t
ORIGIN      
        1 atgtagcatc attactaaaa atacagtcta ttgaagttta tgaagtagct acattctact
       61 ccatgttcaa catgaaacct gtgggtaagt gtctgataga agtttgccgc acaggacctt
      121 gttggctgat gggtgcagaa gatgttgttc gttatattga aaagaaatta ggaatcaaag
      181 ccggagaaac aactgccgat ggtatgttta ccttaaagac agttgaatgt ttggcaagtt
      241 gtggcacagc accaatgatg caaatcggag aaacctatca cgaaaatctg accttagaaa
      301 aagttgatga tattttaaat gatcataaaa accagggcaa cagaagaacg cactgggaaa
      361 aagataaagt aaaaccctga aagaaaaagt atgtcacgta aattactttt agataactta
      421 gataagccgg gcattcagtc aataaccggc tacagcgaca atggcggata cgaagctttg
      481 cgcaaagcac ttcaaatgaa gcctgaagaa attgtaagtg aagtacaaaa atcaggattg
      541 cgtggacgtg gcggtgcggg ttttccaacc ggtatgaagt ggagtttcat tgctaagccc
      601 gaaggtgtgc cacgttattt ggtgtgtaat gccgatgaaa gtgaacccgg aacttttaaa
      661 gaccggtatc tgatggaaca caaacctcat attttaattg agggaatgat aatttcaagt
      721 tttgccttag gggcaaatac ttcctacatc tacatcagag gtgaaatgat gtatgtattt
      781 cacatacttc aaaaagcaat tgacgaagct tatgcagcag gatatctcgg aaaaaatatt
      841 ttaggaagcg gctttaatct cgacctgcat gtacactgtg gtgcaggtgc atatatctgt
      901 ggtgaagaaa cagcattgat agaatcatta gaaggtaaaa gaggaaaccc aagaattaaa
      961 cctccttttc cggcagtgaa aggtttgtgg ggatgtccaa cagtggtaaa taacgtagag
     1021 accattgccg cagttgtacc gataataaaa ataaccggtg aagaatatgc aaaaatcggt
     1081 atcggaaaaa gtacaggcac aaaattaatt tccgcatcag gacacattaa taaacccgga
     1141 gtttatgaaa ttgaactggg tgttcctgtt gaagaattta tttatagcga tgcatactgc
     1201 ggaggaataa aaaacggaaa acagttaaag gctgttgttg ccggaggctc ttctgttcct
     1261 gttctacctg cacacctcat tttaaaaact gcagcaggcg aacctcgtct gatgacttat
     1321 gaaagtcttg ccgatggtgg atttgctaca ggcaccatgc ttggttcagg tggttttatt
     1381 gtgatggacg aagataccag cgtagtaaaa aacctgtgga cgtttgcgca tttttatcat
     1441 cacgaaagtt gcggacaatg ttcgccatgc cgtgaaggaa caggatggat gtacagaata
     1501 ttaaataaaa ttctaaacgg caaaggtgaa atgaaagata ttgatttgtt gtggaacatt
     1561 cagggaaata ttgaaggaaa aacaatttgt cctttaggcg atgcagcagc atggcctgtt
     1621 gcatcatcaa tcagacactt cagaagcgag tttgaagctt atatcaacaa tcacggaatg
     1681 aaagacgtga aacattatta cagactaaat aatttacaag aagcataatg ccaaaagtta
     1741 ctatagatgg tcacaccatc gaagttcctg aaggaacaac catactggaa gcagcccgaa
     1801 aaataggtgg tgatattgtg ccaccaacaa tgtgctatta ttccaaattg aaaacgtcag
     1861 gtggttattg ccgcacttgt ttggttaaag taagtaaagg ctctgaaaaa gatccgcgac
     1921 caatgccaaa acctgttgca tcgtgcagac agactgtaat ggatggtatg gaagtacaaa
     1981 acattacatc gcctgaaatt ttagatgcac gcgcaggagt agttgagttt ttattaatca
     2041 atcacccact tgattgcccg gtttgtgatc aggcaggaga atgtaatctt caggatttaa
     2101 gctacgagca tggccatgaa gtgtcacgtc ttgatttcag gaaaagaact tttccgatga
     2161 tagatattgg cgataaaatt aaactgcaca tgaaccgatg tatcatgtgt tatcgctgtg
     2221 tgaaagttgc tgagcaatta tgtcctgaaa gagttcatgg tgttttaaac agaggatggg
     2281 attcagaaat atctacctac attgaacaag ctgttgacaa tgagtggagc ggaaacatga
     2341 ttgatgtttg tcctgttggt gcattaaccg ataaaacatt ccgtttcaaa agtcgtgtgt
     2401 ggtttaccaa acctgtagat gcacatcgcg actgcccaac atgttcaggc aaagttgtat
     2461 tatggtatca gggagaagaa gtgcttcgtg ttacaggcag aaaagatcaa tggggagaag
     2521 tggatgaatt tatttgtaat gaatgtcgct tcgataagaa aaaaacaagc gactgggtta
     2581 ttgaagggcc acgtcacatt gaccgccatt cagtaatctc acaaaatcat tacgaagagt
     2641 tgaaaaaact gaagcaggat ttatcacgtc agaaaaaatt aggagaaaaa gcacataatt
     2701 aaattttaga aatggaagtt tcggtattaa tattcaaaac agtttttgta attgctgtct
     2761 ttctggtttc attgctgata gccatgtatt cgacctatgc agaaagaaag gtggcggcat
     2821 ttttacaaga cagagttggt cctaaccgtg cagggccatt cggtattctt cagccattag
     2881 ctgatggtgt aaaaatgttt atgaaagaag aaattattcc atcacatgcc gataaagcat
     2941 tgtttatatt aggtccaagc ctgatgatgc ttacagcttg tatgacaggt gtcgtaattc
     3001 catggggcga tactttaatt atcaatggaa aagaatactt tatgcatatt accgatgtaa
     3061 acattggtgt actatatatt ttcggtgtgg tttctattgg tgtttacggt ataatgattg
     3121 gcggatgggc atcaaacaac aaatactctc tgcttggttc ggttcgtgca tcgtcacaaa
     3181 tgatcagtta tgaacttgca atgggaatgt caatcattgc attgataatg gttacaggca
     3241 ctttgagtgt tcgtgaaatt gttgttcagc agcagggctt tcactggaat gtattttatc
     3301 agccattagg atttttaatt tttctgattt gtgcttttgc agaatgtaac cgtacaccgt
     3361 ttgatttacc ggaatgcgaa acagagttgg ttggaggcta tcacacagaa tactcttcga
     3421 tgaaacttgg cttctattta tttgccgagt acatcaatat gtttatttca tctgctgtta
     3481 tttctgcact ttattttggc ggattcaaca tgccatttat cagtgaactt ggattatcgc
     3541 ataatgcatt gacattgcta ggtgtattat tctattttat aaaaatattc ttctttatct
     3601 ttttctttat gtgggtaagg tggacaatac cccgcttccg atacgatcag ttgatgaacc
     3661 ttggttggaa aatgctcatt ccattatcta ttctaaacgt tttgctgacc ggtgtgttct
     3721 tgttatggaa aaacggactg tttcattttt aagcatcaga tgaaaagtga caaaagaatt
     3781 attaatagta ggaggaccaa atggaagtgg taaaacaact tttgcaaaat cgttcttaca
     3841 ggaatataaa tacgaatttt taaatgcaga tgaaattgca agagaattat caaatgatgg
     3901 tagcagggca ggaaatattt catcaggaaa agaatacttt aaacggatag aaaaattaaa
     3961 agcaaaaaat aaaaacatca ttcttgaatc aactttgtca ggtttgtttc tgagaaaatt
     4021 gatcagagag tttaaaaaga aagattacag tataaaaatt gtttttatag tattaaatgc
     4081 tccggaacaa tgtataggaa ggataaaaca tagagttgaa acaggaggac attttgtacc
     4141 agatgatgat gttaggagaa ggtttacaag agggaaaaac aacttctgga aaatttataa
     4201 agatttggtt gaagactgga aattatttaa caacacagaa tcaggatttg agataatagc
     4261 agccggagag aagaataaat atgacattgt caatcaatca ttatttgatt tatttataaa
     4321 agatattgaa aaatgaaaag tagtaagttt aagaattata aaaacagcaa tttgtttaaa
     4381 gaagcattgg aatttgcagc catcggtaac cggggtgtgc aattagcaat gattgattct
     4441 aaaaaaagaa aaataccaac tgttttcgga atcatggata tggtttttta cagactgcca
     4501 gatggttcta taactacaaa atcaccattc aaaaaaagaa aaaagtaaac aacattcatt
     4561 acatttgtaa acggttatta ttataattaa tcatgttaac caatagaagt aaagtagtag
     4621 tcaacaaaga aatgacattt gcagagaaga tatacattcc tgcaattgta gcaggactga
     4681 tgattactat taaacatttt ttcaagaaaa aagcaactat taattttcct gagcaacaaa
     4741 agccaataag tgaagtttgg cgcggcctgc atgttctgaa acgcgatgag caaggtagag
     4801 aaaactgtac tgcctgcgga ttgtgtgcag tagcctgccc tgctgaagcc atcactatga
     4861 cagctgctga acgcaaaaaa ggagaagaac atttgtacag agaagaaaaa tatgctgctg
     4921 tttatgaaat taatatgctg cgctgtattt tctgtggatt atgcgaagaa gcctgcccga
     4981 aagaagcaat atttttaacc aaccgcatga caccatcgga tactttgcgc gataatttta
     5041 tttacggtaa agacaaattg gttgaaggta ttgcaccgga agaacgagta gatataagct
     5101 acagagcaag agcacataga aaagaagtag aatgatgggt tctttgaaaa aacttttttc
     5161 cggagttatt atcactttat cactcggctt aatgtcttgc gataaagatg tgtgcaggga
     5221 ttgctatttg gtgcgttcga tgttgtgtac ctataccgac actacgcttg cagcatatat
     5281 tatacccggt gacaccgtaa aaactgagca gcaatgcgat aaagaaaaag attttaaaga
     5341 cgataaattc aaggaagaaa aatacgaata tgattcacca agcatgtttt atcccaatta
     5401 caatgtacat gtagttgtga ctgacatata tcattgcccg gaaaaaacaa attaatcaat
     5461 agcaaataat caattaaaac aataatttcg caccagtttt tcattctata atactaacgt
     5521 gagccaatac gcattttaca tactttcagc actttcggta atgagtgcct tgtttaccgt
     5581 attttctaaa aagccggttt acagtgtatt gtatctgata ttcttcttct ttacattatt
     5641 cggtcattat attctgctta atgcacaatt tctggctgca gtacatttaa tagtttatgc
     5701 aggtgctatc atggttttgt ttttgtttgt gattatgatg ctaaacctta ataaagaaat
     5761 tgaacccaat aaaaatattg tttcaaaatt tgcagccgtc atatccagtg gtctgttgct
     5821 gattgtaatt gtaagtgtac tcaagcatgt ggatgtagaa tcttccaaat tgaactcaca
     5881 aacagatata ggtttggtag aatccattgg acatgtacta tacaatgaat atatgctgcc
     5941 atttgaaatg gtttcaatat tactgctcac tgcattaatt ggtgctgtga tgcttggaaa
     6001 aaaagaagtt aaataaaatg gaaccggtta atataaatca ctacctgctg ctctctgcca
     6061 tacttttttg tattggcgtt gccggagtat tattcagacg caatgccata attattttta
     6121 tgtgtattga actaatgctt aactctgtga atctcctatt gaccgctttc agcaatcagc
     6181 ataatgatgc atcgggtcag gtgtttgtga tttttattat ggccgtagcc gctgccgagg
     6241 ttgcagtagg attagctatt ctggttatga tttatcgtaa tgttaagtca acagatatag
     6301 atgttttaaa taaacttaaa tggtaggtaa tggatttttt gatatcagca attcctcttg
     6361 ttccgctcat cggttttctg cttaacggca taatcaataa aaaaatgccg caaagtcttg
     6421 taggcattat tgcaagcggc actgttctat tttctttttt aatttcctgc tatctctttt
     6481 tgcaaatcaa caatggctcg attaaggaat cgtattattc catgttttcg tggatcaatg
     6541 caggtagcct caatctgggt ctttcatttt tagttgatcg cttatcggtt ataatgcttt
     6601 tagttgttac cggtgtaggc tttctgattc atgtttattc tattggctat atgaaacatg
     6661 atgaagatta ctcaaggttc atgtcatatc tgaatctgtt tatctttttc atgctgctgc
     6721 ttgtaatggg aagtaactat gtagtaatgt ttgccggatg ggaaggtgtt ggtctttgct
     6781 cctatttact gataggtttc tggtttaaaa acaacgacta taataaagca gcaaacaaag
     6841 cattcatcat gaatcgtatt ggtgatttag gatttttact tggcttgttt ttaatgtatc
     6901 agacattttc atcattagaa tttaaagatg tttttgctgc tgcaggaaac tatccttcag
     6961 gtgatgctac gctgactgca attgcattgt ttctttttat tggtgcagta ggaaaaagtg
     7021 cacaaatacc actctatacc tggttgcccg atgccatggc aggacctaca cctgtatcag
     7081 ctttgataca tgctgctacc atggtaactg ccggtgttta tatggttatt cgcagcaatg
     7141 taatttatac gcttgcacca tttgcaggag agattgtggc tgtaatagga ttagtaacag
     7201 cattgatggc agcatcaata ggtcttaaac aaaatgacat taaaaaagta cttgcctact
     7261 ctaccgtaag tcagttgggc tatatgtttg tagcattagg tgttggtgct tataccactg
     7321 ctctgtttca cgtagtaaca catgcatttt ttaaagcatt gcttttctta tgtgccggta
     7381 gtgttattca tgccatgagt ggcgaacagg acatcagacg catggggggt ttgaaaaaat
     7441 atttaccgat aacgcatttt actttcctga tgggagtttt ggcaattagt ggtgttccgt
     7501 tgtttagcgg attcttttct aaagacgaaa ttcttgcagt aacctggact tattcaccgg
     7561 tgatgtttac catattagca atcacatcgg tactaactgc aacctatatg ttccgtttat
     7621 actggttgac tttttatggt gattttcgtg gcacacatca tcagaaagag catcttcacg
     7681 aatcaccttc gagcatgact gtaccattaa ttattcttgc tgttttctct gtaataggag
     7741 gttatatcgg catgccggaa tatatcaatg aaaaattagg aacgcaccac gtgtttacgc
     7801 agttccttaa tccaattatt tattctccaa aagaatttat tttatcgcat acatttgagt
     7861 ttactttgtt gataggcact ataattggat tggccataat ttatgttatg actaaaaata
     7921 tgtatgtcag agataaagtg gtgcctgaag atgacagtca aacaaaatca ttccaaaaag
     7981 tacttgccaa caaatattat gttgatgaat tatacgatat gataatcaga aaaccgttag
     8041 aagcgtttgg cgaattgttt tataaaatat ttgacattag tatcattgat ggaattgtaa
     8101 attttatacc tcgagccaca aacctgactt cttccgggtt aagattatta caaaacggaa
     8161 gtgttggatt ctatttggtg agtatggtca taggaatcat tttgattttt gtatttaaaa
     8221 tgctgctgta gatgaataat ttattactga taattttatt tttaccactt gccggcatgg
     8281 cattgtcgct gttgccttca cgtgaagcga caggaaaaac agcatttgta ttttctctca
     8341 tcagtttatt gctttcagtt tgcttgttta ttcagtttga tcctaatggt ggaatgcagt
     8401 atgtaataaa ttatgcatgg gtgccggatt taggaataaa tttcaaaata ggtgttgatg
     8461 gaatttcgtt gatgatgatt ttattgacca acgtactaac accattgatt attctttcat
     8521 cttttggacg cagaatagaa aatcctaaag gactttatgc actcattcaa ctgatgcagt
     8581 ttgctttggt tggtgtattt atttcgctcg atgcatttct attttacatc ttctgggaac
     8641 ttgcactatt acctatttat ttcatttgcc tgttgtgggg tggcaaagac agaatacgaa
     8701 tcactttaaa atttttcatc tacaccatta ccggaagttt attaatgctt gtaggtttaa
     8761 tttacctcta tctgcaaaca cctggaagtc acacattcga tattcaggca ttttatgatt
     8821 taacgctttc atcacaacaa cagacgttag ttttctggtt gatttttgtt gcttttgcaa
     8881 taaagattcc tgtatttcca ttacatagct ggcaacccga cacctatact gatgcaccaa
     8941 cacaaggtac gatgttactt tccggtatca tgctaaaaat gggtctttat ggtgtgttaa
     9001 gatggttact gcctattgtt cccggagcat cagcagataa tgcatttatt gtaatgttac
     9061 ttggaatcat cggtgtggtt tatgcttcat gcattgccat agttcaaaaa gattttaaac
     9121 ggatgattgc ttatgcatct attgcacacg tgggtttaat tactgccgga ttattcactt
     9181 taaaagttga agcgcttgaa ggtgcagtat ttcaaatgct cagtcacggt attaatgctg
     9241 ttggattatt ctttatagct gatatgttga tgagcagatt aaaaacacat aacatgagtg
     9301 aaatgggtgg cattgcaaat aacaattctc tgtttgctgt tctgtttatg atagtattat
     9361 taggcacggt agcacttccg ttaactaatg gttttgttgg agagtttctg ttgcttacag
     9421 gaatttatca gtttaaagcg tggatggcaa tatttggcgg attgacaatc atactcggtg
     9481 cagtatatat gttcagaagc tatcaggcta tcatgcttgg cagccgtcat ctgaatgaag
     9541 gagagtttca tccaatgaca ttgagtgaaa aaacagtttt aggaactata gcattcttgg
     9601 ttattttatt aggtgtgtat ccatcttttc tgatgaatgc agcagcacca agtataaaat
     9661 cgctgttaat gattattaat gcaggataat gtttaaaaat aaattaatct aattgtgaat
     9721 accatcgtta cactttctat agtcggcatt gcagccatgc tttcagaagt ttttaaactg
     9781 aagcgcataa tgtacacgct ggtgattgcc ggtttagttg ttgcattagg tttttcaata
     9841 aacgactgga ataatttcgg ttcgtacttc aatcagatgg taatggttga cagtttttct
     9901 atagtttttt catcactcat gattgtaaca accttgttgt ggatgattat gtcgcgtagt
     9961 tactttagta gtgatagtag catgagcgag cattttgcac tcatcgtttt tgcactcacc
    10021 ggtggtgtga tgatgacaac tttttcaaac atgtccatgc tttttcttgg attggagata
    10081 ttgtcgtttg cactttacat catggccgga agtgacaaaa ccaatatcaa gagtaacgaa
    10141 gctgccatga aatattttat catgggttcg tttgcaacag gctttctact ttttggaatt
    10201 acactgattt atggagcaac atcatctttc aacattcagg aaattgcaaa ctatacacaa
    10261 caaaataaag atgcactacc tgcttttttt aaagttggtg tgttgatgat attaattggt
    10321 ctgtcattta aaattgctgc tgcacctttt catttctgga cacctgatgt gtatgatggt
    10381 gcgccaacat tagtgacagc gtttatggca tctgtagtaa agactgcagc ctttgctatg
    10441 atttacaggt tatttgtttc atgcttcata tctatttatg atttctggac accggtattt
    10501 gctgttctgt cagccataac catgctggcc ggaaatatca cagcggttta tcaaacaagc
    10561 ctgaaacgaa tgcttgctta ttccagtgtt gcacatgcag gctacatgct catggcattg
    10621 gcagcaccgg gcattgcttc aaaaaatgca ttgttgtttt atctggcagc ctattcactt
    10681 tctgcattag gtgcatttaa tcttctttac tcagtacata aagttacagg aaacgaagga
    10741 ataaaagctg tgaaaggttt gttttataaa catcctgtat cagcaattat gctcactatt
    10801 tgtatgctga gtatggcagg cattcctcct gttgcaggat tttttggaaa atattatttg
    10861 tttacggcag cactaaaaga aaatcttctg tggttggttc tcattgcaat attaagttcg
    10921 cttatcggaa tttattttta ctttaaagtt atcattgcag catttcaaca acctgaaaca
    10981 gaaaacactg gctactgcga aagaccatcc gatcttgccg tattgggcat ttgcgcagtg
    11041 ctttcattaa ttctaggtat agctcctcaa ttaatttccg gattgctctg ataatacatt
    11101 ttacctaccg agtaaaagct ttcagataaa ctttactttt ttttccaatt gtacttcagg
    11161 ccaagtgcaa accattttcc aggcattgga acagcacctg cttctttata ctttgcatca
    11221 aacatattag agacatttgc ataaaataaa acagaatgaa tttcataact tatgcgtccg
    11281 tcaacaatat tataagcatc gtttagctcc cgcttaatat atctgttttc aatttgaaca
    11341 ttaaaactac cgtaacgata attaacaccg gcaatcaatt gatgtcttaa tgattccagc
    11401 acatatttag actgttcgtt tttataatca taatgttcag ggtttaaaaa attataactg
    11461 atgcaaaaat tcaattgatg ttttccgctt agaatcaact gttgattaat tcttgaataa
    11521 ataccttgta caatgatatt gccaaaatta cttggtgaat acggtgttgt catgtttggt
    11581 cgtacccaat caataaagtc tgtaacctta cgatagaaag accctacatt tactttcaaa
    11641 tgcccggaag taaattcaat gttcgcatca tactgccatg cagtttccgg ttgcacatca
    11701 gggttgccca catttgccgg aggttgtttt aaatacaagt ctgtaaaaga tggaattcgc
    11761 tgacctgagc caaaacttgc tgcaattttc catttgttat ttaaacgata agctaaatcc
    11821 aatccgggat aggctttcca atcaaactgt gaattataat ttacgtatat gccgacatta
    11881 gttactaatc tcttaaaaag tttggttctg aattcaataa aagctccatg attgtccatt
    11941 ttatgttctc caatattgga actgttaatc tttgtcaaac gcgactccca acccatacct
    12001 attacacctg cagatgttgt aaggttaccg tttagttcca gcatcagtgc attggtaaaa
    12061 tgttttgacc ttcctctgct taaatcactt ttaaaatagc gataatcatc ttcatcataa
    12121 cggtcactta ttcgaggcat taaattgact tttccaagct gatgtttaga ggataaacta
    12181 aaaattgatg tttctacaat ctcttctgaa ttcttatcac caggagcagc ataaaaacca
    12241 ttagctccaa atttgttata ggtatgtccg gcaaaagtgc ttatggaatt tttttcattt
    12301 atataaaaat caccatcata aaaaatcttt gtgtttttat gtgccgaatt atatctgtag
    12361 ccattaaata aattctgact tagacctaaa agttgtctat gattattatt tgcaaagttt
    12421 ccaaccaagt caacccctcc acctccatac atgccgtcac catcaccggt ttcttttctt
    12481 ttaaaagatg tacctccgta aatatttgca gtaacaaatg attcattact ttttttagtg
    12541 actatattaa cagcaccggt gagtgcattg ataccatata cccttgctgc agcaccgcga
    12601 agtacttcaa tgtggtcaat agcatccaac gagattggta agttcaatac attatgtgct
    12661 gtttgcgcat taatcagttt gataccattt agcaaaacca aggtttgctc aaatgatcct
    12721 ccatcaatac taatgtcgcc ttgtgtgcca aaaggagcac gctgacgcat atcaacacct
    12781 gatacataaa caagcaattc ggttaaagtt tgaaccggca aagctgcaat ttccttctgt
    12841 gtaattatct gaatatctct tgctgcttca ctaaaaggtg tttctaaacg attaccttgt
    12901 ataattattt ctttaactgt atgtgttgaa atgctatccc tttcatcttg agaataagct
    12961 gtgaaaacaa taaaaaataa cattaaaagc aacaacgctt tttgcagctg tatattcaaa
    13021 tttttcaaaa tatattttat aaatagatgt gtttaaaaaa gtttttgtct tttaaattgt
    13081 attccaactc tcaatactaa ttgaaaatat ctcgagttta aataataaat ttcatcagtg
    13141 ttagttgctt ctctgaaaaa aaccgccatc atttttagta taacagcggt ttccttgtat
    13201 aagatttatg ttgatttctt aaatcttaac tctccaattc aaaaaatcaa taacttacta
    13261 aactaaaacc caacaacaac tttatgtgta gttgaagtac gtccgtcagt tactttaata
    13321 aaatatatgc cttccgcttg attatttaaa tcaacctcca aagattgccc catgtttgtt
    13381 tgttgaaaat taactttctg tccgagggca ttcagaactt ctattgtagc attagtgctg
    13441 ccaacattac ttaacaaaaa tttaccggaa gaaggattag gtgtgatggt catttctgta
    13501 tgtgctgcca actgatttaa gcctgtagtt cctgaaacag gttgatagcg taaaacaggt
    13561 gtttgcgctg taccgtcaat ggttgtaata ccataggtaa gtaaaggttg tttgatggta
    13621 tcatgaaacc aatagtagta atcatcagaa accaaataat caattaccgg agtacctaac
    13681 atataaattt ttatatgaaa tgtttcaatt gcatgaatac gcaacacatt agtgaatgtg
    13741 cctgttggca aaatcaaagt tccttgctga tcggcattga ttgcaagcga tccgctttca
    13801 agtgtgcagg aatcttcgcc caacccgaat ggtgcatagc gtccggagta agtagaatta
    13861 actgtttggc ctgctgtcat cggaaaatga aattctgtgg cataggtacc tgtattataa
    13921 acgccataat tagatgcgct gctataccat ccaattcgat tgatatcatt tgcagtattc
    13981 tgataataaa aataacctgc tgcaccactc tcaccacgtg ctaatgttgc accgggaaaa
    14041 gaatcaacgc cattacctgt tattgttgcc gggtcgtcat agaaaaaact gtagttggtg
    14101 cctgcattca taagcagtgc attatcccaa agtgtattag tgacagggcc tgtaccgttt
    14161 tggttgaatc caaatgtgtt ggcatcaaca taccttatag tatctccaat agcaggcatc
    14221 tgatgcgttg cagagatggt aatgagctgt gcatttgtat taattgcagc agcacaaaat
    14281 agtgtggtaa tgagtgtaaa tgttttattc atgttatcat tttttgtgaa gcaatattaa
    14341 aaaaataatt caatgttgca tgaattcttt ttttcataaa gaagcatttc tatacgtcag
    14401 gaacaatctg ctacatacct aactatttat gctgacacaa gtcattttat atttcatccg
    14461 tttctgccat cttgcacacc tttattttac tttataaata atgacagaaa taatgtcacc
    14521 tgcaggctcg ttcgagtcgt tgcaagcagc tatacaaggt ggtgccgatt ccgtatattt
    14581 tggaatagag cagctgaata tgcgtgcacg tgctgccaat aattttacac tcagtgatct
    14641 tgctgaaatt aaaaaaatat gcagcgaaaa aaatgttaaa acatacctta cactaaacac
    14701 ggtaatgtat gatcacgaca ttatgctgat gaaaaaaatt attgatgcag caaagaaaaa
    14761 taatatagat gctgttatag catctgacca tgcggcattg atgcatgcta aaaaaattgg
    14821 tatgcctata cacatttcaa cgcaggccaa tgtcagcaat attgagagtg ttcgcttttt
    14881 tgcagactat gccgacacaa ttgtgttagc acgtgaatta acattgaatc aggtagccga
    14941 tgttgtaaaa cagatcaaac gtcagaaaat aaaaggtcct tcaggtaaat taataaaaat
    15001 agaagtgttt gcccatggtg ctttatgtat ggccgtttct ggcaaatgtt atctgagtct
    15061 ccactcacac aatgcctcag caaaccgtgg tgcatgtatt cagaattgca gacgtgaata
    15121 tattgttcag gataaagaaa acgaagttga gcttgaaatt gacaatgagt atatcatgtc
    15181 gtcaaaagat ttgtgcacca ttgattttat tgacaaacta attgaaacgg gcatcagtat
    15241 tttgaaaatt gaaggcagag gtcgtgctgc cgactatgtt tatactacaa caaaatgtta
    15301 taaagaagct gttgaatgtt atcataatgg cagctattct gcaacaaaaa ttgaacattg
    15361 gaaaaatgaa ctggcaacag tctttaaccg tggtttctgg ggtggttatt ttcttggaaa
    15421 aactatgggt gaatggagca ataccggagg ctcgcaggca acgactaaaa aaatttatgt
    15481 cggtaagggt gtcaagtatt ttccaaaaaa caaagtaggc gaatttaagc tggaatcagg
    15541 aacactatcc gttgacaatg acattatgat taccggttca acaacaggaa ttattcttac
    15601 caaggttcag gaattgcgaa tggatgattt acaggttaca actgtaaaaa aaggtgatgt
    15661 gttttcaatg ccagttgctg agaaaataaa accatcagac aaactttata aaatcgttaa
    15721 tgattgaaat agtacaatac aggagtaaat gtattggatg caatgcctgt gttgaagcct
    15781 cggcaacacg atggcgtatg tcacgaaaag acggaaaatg tactcttgta ggtggaaaaa
    15841 ataataaggg catctacatt gctgttgtaa acgatgatga atatgaacaa gcactgcagg
    15901 cagcaaaaaa ttgtccggta aatattatta aagtaaaaaa gagataaatg gatgcagcaa
    15961 tagaaaattt tatcagtgcg caaaaaaata tgacattatg cacttcggtt aacgacatac
    16021 ccgaatgtgc aaattgtttt tatgtattca tgcaggacgg tgctcatctg atatttaaat
    16081 ccaaaaaaac taccggtcat gtttctcatg cacttgtaaa taataaagtg gcaggcacaa
    16141 tattaccaga cataagtaaa attggaaaca tcaagggcat tcagttttca ggaatttttc
    16201 ttgaaccaca agatgtcatg ctggagcagg cgaagaaaat ttattacagt aaatttcctc
    16261 tggcattagg aatatcgggc gacttatggc tgataaaatt aacctacatc aaaatgactg
    16321 ataacacgct tggctttgga aaaaagctga tatgggaaaa caagaactga tataaaaaat
    16381 caattaaact gcttctgcgg tttcgtaagc cgaaacatca gggcaagtgc atatcaggtt
    16441 tctgtcacca taagcattat caactctttt caccgatggc cagaatttat ttcctgcaac
    16501 ccatggtgcc ggaaatactg ctttctgacg tgagtaacta tgcttccatt catctgctgt
    16561 tgcttcaagt gcagtatgtg gcgcattcac aagcatatta tcttctttgc tgtactcacc
    16621 attgattact tccataattt catgtctgat agcaatcatt gcatcacaaa aacgatctat
    16681 ctcttctaat ggctcactct ctgtaggttc caccataagc gttcctgcaa caggaaatgc
    16741 tactgtaggt gcatgaaaac cataatccat cagtcgcttg gcaatatcag ttacatcaac
    16801 attggcaaca gctttaaatg cacgacaatc taaaatcatt tcatgagcac aaaaaccatt
    16861 tttgccttta tataaaacag gataatgttt ttctaatcgt gctttaatat aattggcatt
    16921 gagcattgct atttctgttg cacgttgcac acctgtctta ccaagcattt taatataacc
    16981 atagctgatt aacaaaatca atgcactgcc ccatggcgct gcagacacgg catttacacc
    17041 cttacgtcca cctgtcggga caacagtatg cgaaggtaaa aatggcgcaa gatgttcagc
    17101 tacaccaata ggtcccattc cggggccacc tccaccatgt ggtatggcaa atgttttatg
    17161 caaattcaaa tggcaaacat cagcacctat caaggcagga catgttaaac ctacctgtgc
    17221 attcatgttt gcaccatcca tataaacctg accaccgttg gcatgtattg ctgctgtgat
    17281 ctgtttaata ttttcctcat acactccatg tgttgaagga tatgtcacca tcaaacaaga
    17341 taaattttcc tgatgctgtt ctgctttaag tcttaaatcg ctcaggtcaa tgtttccatc
    17401 atcatcacat ttaacaacaa caacttttaa tccggcaagt acagcactgg caggattggt
    17461 tccatgtgca gattgtggta tcaataccac attgcgatga ctctgtcctc tgtcgctatg
    17521 atatttctga ataacaacta aaccggcata ttctccttgt gcaccgctgt taggttgtaa
    17581 tgacaccgct gcaaatcctg ttatctcttt cagaaaattt tccagttcag aaataatatg
    17641 tgcataacct tgtgcttgtt ctataggagc aaatggatgc atgttggcaa actcaggcca
    17701 gcttagtggt tccatctcac tggtggcatt tagtttcatg gtgcacgaac ctaaagatat
    17761 catcgaatga ttcaatgcaa gatctttatt ttcgagacgt ttgatgtagc gcaacatttc
    17821 tgtttcgctg tgatgtgtat taaatactgc attggtcata tattccgaac tgcgtaatag
    17881 cgcagcaggt atggcattac cattcatagt tgcagcagca atagactttt taccaatact
    17941 tgcagcaaac acacgcaaaa tatctttcaa atcttcgtca gaggttgttt catctatact
    18001 aacacccact gtatttccat tgtaacggaa gtttatcaga ttagcttcag ccatatcgcg
    18061 aacactctct gtatcttcta cttcaacagt caatgtatcg aaccatgagt tgttcactac
    18121 tttaaatccg gctttcttta aactctctgc aagtgcagta gtacgcttat gtactgtagt
    18181 agcaatagcc ttcaaacctt ctgctccatg atacactgca tacattgatg ccatcactgc
    18241 taacagcacc tgcgcagtac aaatattact tgttgcctta tctcgtctga tatgttgctc
    18301 gcgtgtctgc aatgccatac gtaaagcagg attgccttct gcatctacac tcataccgat
    18361 aattcttccg ggcaaatttc ttttatatac atcatgtgtt gcaaaatatg cagcatgcgg
    18421 accgccatat cccataggta caccaaaacg ttgtgttgat cctaccacac aatcggcacc
    18481 ccattcaccg ggaggcgtta acaatactag acttaaaata tctgctgcta cacaaacacg
    18541 aacttcatgt cggtgacaca tgttaacaat gtttttataa tccaacacat tacctattgc
    18601 atcaggatac tgaagtaatg caccaaagaa actgtcatca ggcgaaaaca attgctcatc
    18661 gccaacaacc aattcaatat ccaatggttt gctgcgtgtg cgcaacacat ctatagtttg
    18721 cggaaagcag ttttcagaaa caaaaaactt acatgcattt tttttctctg cctctttaga
    18781 gcgtgtataa taaaacatca ccattgcttc tgcagcagca gtgccttcat ccagcagcga
    18841 tgcatttgca atctgcatac ctgtcaaatc gcacaccata gtctgaaaat tcagcaatgc
    18901 ttccatcctg ccctgcgcaa tctctgcctg ataaggagta tatgctgtgt accaacccgg
    18961 attttcaaaa atgtttcgtt ttattacacc cggcacaatg gtattgtaat accccatgcc
    19021 aataaaattt ttaaaaacct gattttccga agctgttgct ttcaattcat ttaaatattc
    19081 aaactcgctc attgcctccg gaaggtttaa tggtttttgc aaacgaatca gttcaggaat
    19141 aattttagca ataagatcat ttacggattt tacgccaatg gtggcaagca tgtgtttaac
    19201 ctgcttgtca tcagggccaa tgtgcctgtc ggcaaaagaa tagtacatgt actctggatt
    19261 ttgccgcaaa agtaaataat tagaacgtta aaaaatgtat ttctgaaagc attcaacatg
    19321 atttacatca agcatttacc gctttttagc acaaaaatta ttaacacatg ttgatatttc
    19381 aatatttcta aaacttgggc atgtcaccgc ctgtggcgct gtcgggctat acgctgcaag
    19441 tccgctcctg cctgcggcag catgcgggct tttcgctact atccctcatg caaaaaacca
    19501 tctccaaaat aaaaactttc ctcctccgat tcgattatct atataacacc acattatcaa
    19561 atttttaaca aatgctatat attacataca ttttttatag ttttgaatca aataactgat
    19621 atgaccttac gtcactttat aacatccctt atttttcttt tcagcgccac aacattaagc
    19681 gcacaagaca cactgttgct ccttaatggc aaaaccaaac ctgtaaaact ttttaacctc
    19741 aacgaccccg actgggtgag atatgtaaaa ttcaaggaga gtgataaaaa gaaaaatgaa
    19801 ggaagtgcgc gtgtaaaacg cattgatgtt tacaaagtgt tctccatcaa aaaagctgat
    19861 ggcacagagc aagttcttta taaaccggac agcacatcag atgatcctga tattccatgg
    19921 gtgcgcgatt ttattgcagg acaacaatat ggaataatgc ataaaagaga gcgttttaac
    19981 aaacacgata accaatggca ccgcagacta aactttactg aagttggtgg aatagctgca
    20041 ggcggtgcag gaagtctttt aactttttat ggtattcctg tgcctgcaat ttatgccatt
    20101 gctgtgggac ggcatagccc tgcattaccc aacgatccgg ctattgctcc tgaaatgaaa
    20161 aacagcgaag gatttatttc aggctatcaa aaacaaagac gtaatcagcg aatcagacaa
    20221 ggatttattt ccggcatgat aggttttgct gttggcattg ttactttcac tctaatagaa
    20281 cggaactgat tgttcaggaa gattgctcat aaagctgttt cctataacct actcattgcc
    20341 cttgcagcaa gtgcagcaag tcatttaact attctcagaa taaatgaaac aaacaatgta
    20401 gttcttatag caattatatt ctttgctact ctggctatct ataattttcc tcgtttgcca
    20461 tggcaaaaac cacttaacac acggacatgg atttgtctta tttcgattct attctcagca
    20521 attttaataa tcaattataa ttatatttcc ataaatcttt ttttgacagc tattctggct
    20581 tgtctcattt atatgatgcc ttttaattac aagggcaaac gcatcaaagg aattcgcaac
    20641 atttttcttc ttaaaaatat ttttcttgca gcaagttggg cattaataac tgtactctta
    20701 cctatggctg cattagggca tgaatcacca ataataaaag aagttttttt tactccgttt
    20761 catttatgta ttcggtattt ctttattgtt tgatattcgc gatactgcaa aagacaaaac
    20821 acgcaaccac ctgacactac ccgtggtttt aggaacaaca gtaacccgat atattgttat
    20881 tgcatctgca gcattattta ttttaattgt ttctgtactt tatcgtcatg aacattttac
    20941 attacctatg gcagttgcat ttatcacaag tgcactgttg ttggtagttg cgacaattac
    21001 tgcaccacct gcagcaaact ttaaatttta tcaggttgga gttgacagtt tgcttattgc
    21061 acaatggtta ttggtggtag ttgccgtaaa attttattaa tttctttcta ttccctcctc
    21121 gttatacaaa tcctctttta tttttttggt aatttaatct taatcatgta ggtttgaact
    21181 ccactaaaaa tcaagatcaa aggtatgtcc aaaggaatac atgtaaaaaa acctctcgat
    21241 agtttattag ctgaagcaga agacagcgaa aaaggtttaa aaagaacgct tactgcaaac
    21301 agtcttattg cactcggtat tggtgctatt attggtgcag gactatttgt gcgcactgct
    21361 gctgctgctg caaacaatgc tgggccggct gttacattag cttttgttat tgcaggattg
    21421 ggttgcgcct ttgcaggatt gtgctatgca gaatttgcaa gtatgatacc tattgccggt
    21481 agtgcctata cttatagtta cactacaatg ggtgaattta ttgcctggat aatcggatgg
    21541 gatcttgtgc ttgaatatgc acttggtgca gcaacagtga gtattgcctg gagcgaatac
    21601 ctgaacaaat tgttgaatta tctgggcatg cacatacctt atgaatggtg ccattcacct
    21661 tttgaacata gtttagatgg tgtttatggt attatgaatg ttcctgcatt ggtaattctc
    21721 atattacttt ccttactctt aataaaaggt actcaagaaa gtgccttcgt aaatggtatc
    21781 attgtagtta ctaaagtggc tattgtaata ctctttattg tttttggctg gcaatttatt
    21841 aatcctgcta atcacacgcc ttatatacct gctgcaagca ctttcaccga ccacatgggt
    21901 gtgccacatc actatggtgg aatattggga attcttggtg ctgccggtgt ggtattcttt
    21961 gcattcattg gttttgatgc cgtgagcact gctgcacaag aagcaaaaaa tcccggtcgt
    22021 gatatgccaa aaggaatttt aggttcatta ataatttgta ctgtccttta cattctgttt
    22081 gctcatgtgc taacaggagt tgcctcagta gaagatttca gaacacaagg ccgtgaagcc
    22141 tcagtgactt atgccattca aacctatatg catggctacg actggttagc aaaattggta
    22201 accgttgcta ttcttgccgg attctcctct gtaattttag ttatgctttt aggacaatca
    22261 cgtgtatttt attctatgag tcgtgatgga ttgttgccta aagttttttc agaactacat
    22321 cctgtttacc gaacacctta taaatcgaat ttaattctct ttgtttttgt cggacttttt
    22381 gctgcatttg taccgggtga tattgttggt gatatgacaa gcattggaac actgtttgca
    22441 tttatgctgg ttgcagccgg tgtaatcatt atgcgtaaaa ccaatcccga tgcaaaacgt
    22501 actttcagaa cacctttagt tccattagtt cccatcttag gaattattgt ttgtggagcc
    22561 atgatattcg gcttgggttg gcctaattgg tcacgcttac taatgtggct ggctataggt
    22621 ttcgttattt attttggcta cagtgcaaag cacagtaaat tacgtaatcc gaaataaaat
    22681 tactttctaa aaatttatat gcacaacgac aaaccacacc tgcaccgttc attaggactg
    22741 tgggatgcta ccatgttggt tgccggttca atgattggct caggtatttt tattgtcagt
    22801 gccgacatgt cacggcatac aggttctgca ggatggttgc tttttttatg gttgctaagt
    22861 ggtgttatca cacttatagc agcactttgt tatggcgaac tggcaggtat gatgccgcaa
    22921 gcaggaggtc agtttgttta tatcagacgt gcatggggaa actgcttggc atttttatac
    22981 ggatggacag tatttatggt cattcaaacg ggagttattg ctgcagtggc tgtagcattt
    23041 gcaaaattta ctgctgtgtt ttttccttat tttaacacag gcaatatttt atttcaggca
    23101 ggcggaataa gtatatctgc tgcacaaatg cttgctatat ttctcattgc tgtactaaca
    23161 tggttcaaca gccgtggtat tgaaggtgga aaaaccattc aaaacatttt tacttcagca
    23221 aagttgcttg ccctatttat tctcattgta gccggactat gggttggatt aaaaaccaac
    23281 acgctttcaa ctaacttcgg caatgcctgg agcgttaatt caatgattaa aaccgattcg
    23341 ggctggatag aacaacacat cagtggcttt gcaataatac tcatgcttgg cactgccatt
    23401 ataggctcat tattcagcag cgatgcttgg aacaacgtta catttattgc cggtgaaata
    23461 aaagatccga gccgcaacat tccacgaagt ctgatgttgg gcacagcaat ggtaacttta
    23521 ctttactgct tagccaacat tgcatatctg gcattattac ctttaaaagg cagtattgat
    23581 gctgcaacag ttgcagggca aggtattcag tttgcaggag gcgacaccga ccgggtagga
    23641 acagccgcag cctctatgat ttttggtgac tcaagtgttt atatcatggc agcactcatc
    23701 atgatttcaa ctttcggatg caacaatggt attatcctta gtggtgccag agtttattat
    23761 gcaatggctt tggaaaatct cttttttaag caggcaaaaa aactcaattc aaaaggagtt
    23821 ccagcaattg cgctatgggt acaggcaata tgggcaggtt tattatgtct aagcggcacg
    23881 tatggtgatt tacttgatta cactgttttt gcctcactcc tgttttatgt aataactatt
    23941 tccggtattt ttattttgcg taaacgtgag cctgatgcag aaaggcctta caaagtattt
    24001 ggttatccat ttttaccggc actctatatt attttagcac tatcaatttg ctctatatta
    24061 ctttatacca aaacagccgt tgccggcaaa ggcttactta ttgttctgct aggattaccc
    24121 atctattatc tgtttaaaag acgcagcagt taaatagatt tctcaggtat ttaaaagcta
    24181 cgtacattcg tagttcaaaa tataaatgtc attcaaatga gaaaaatctc tacactattt
    24241 gctatgttgt gtattacaac tgctgtttca gcacaaacac aacgccttgt tcttttcgag
    24301 gaatttaccg gtgaaacctg cgggccatgt gcactggtaa atcctacact caacgaaatg
    24361 cttaatgatg aagaaacaaa aattgtttct attaagtatc aaaacaacat tccttctccc
    24421 ggtccgcgat tctggcctta tgcacaatca gatattgcag cccgtcaggc atattacaac
    24481 aacacttatt cacctcatgg aattatggat ggcaatcagt ttaacgataa tgcaggtgct
    24541 ttagatcctg ttatcatcaa cccacgttat gcaacacctt caccatttga agttagggtt
    24601 gctcatactt tcagtgcagc caacgacact atttatgctt ctgtaataat aaaagcagca
    24661 caaagttata gcgactcaaa cttggttgca cagattgcag ttaccgaacg agatattttt
    24721 ggatacacat cacctaatgg tgaaacacac tacgaaggag tacttcgtaa aatgttgcct
    24781 tctgcatacg gaacgccatt gcaaatctca tggaatgcag gcgattctgt tgttctaaat
    24841 tattcgtggt acatcacacc tgctgcactt tcatctcctg tttattatca gctatcagta
    24901 gttgcatttg ttcaggacaa ctctagcaaa gaagtacttc aggcaggttt cagtcgtgca
    24961 caagttccta tggacccaaa agcaaaagca ataacaggca tgcaggatgt tacttgcagc
    25021 ggtacaatga caccatcatt tacagttgca aacaatggca taactgacat ttcaacttta
    25081 gacatacaat ataaagtaga taataatcct gcgcagacat attcctggat gggactgttg
    25141 ccaagtttta caagtacaac catttccttg ccaccaattc cggtttcagg aaatggaagt
    25201 catagtatta ctttaactac agcgaatcct aatggcggtg ttacggataa taatatgctt
    25261 aacaatagca aaactgctta tttcggtatt ccgggtgctg ctgttgcagg aaatgttttg
    25321 gaggatttta ctttatcaac tttcccgcca gctaactgga ttcgtgttga tcagaatgga
    25381 aacaataccg gatggttacg ttcaactgca ggaaatggat ctgctaaaat tgatttttat
    25441 agcagcttta ccggtcacat tgataatctt tatctatatc cggttgacat gagtggtgca
    25501 tcgggtgcta ctatgacttt tgatgtttca catcgtcaat atagtgctgc ctatattgat
    25561 gcaataaatg ttcaggtttc tacagattgc ggactaacat ggaatacaga atgggctaaa
    25621 tcgggtgccg ctcttgcaac agttacaggc tatcagacag gcgcctttac accttcagca
    25681 gcacagtggc gtgctgaaac cgtaaacctg aatcaatatg caggcaacaa caatgtactt
    25741 attcgtttca atgcagtgag tggttatggt aacaatggtt atgttgataa tgtgaatgtt
    25801 tctttgtcaa caggaatcaa tgaagttgct aatactacat ttgaattata tccaaaccct
    25861 tcaactggaa ttgtaaccat cagaactaaa acacaaaatt ctgattataa aatccgtttc
    25921 actgacattt cgggaaaaga agtttttgta aatactcgtg ttgaaggcaa taccattaaa
    25981 gctgatttca gtgcatgcag caatggaaca tactttgccg aaatacaaac accaaacggc
    26041 acagaacgca gtaaattcat tttacttaaa gattaatctt taagtaataa aattgtaata
    26101 attacaacta agccatcagt atgctgaaat actgatggct ttttgatttt acatcttaga
    26161 atctattagg caacaaccat ttctttagca tatcttttta cctttgcact ctcaaaaaat
    26221 tttaatttaa aatcaaactt agttatgtca aaaggaccca tatcgcaatt tatgcagcat
    26281 cactaccgtc attttaatgc tgctgctatg atggatgctt caaaagccta tgttacacat
    26341 cttgccgaag gtggaaaaat gatggttaca cttgccggtg ccatgagtac agcagaactt
    26401 ggaatttcac ttgcagaaat gattcgtcag gataaagtgc atattatttc ctgtaccgga
    26461 gcaaatcttg aagaagatat tatgaacctt gtagcacaca acagttataa gcgagttcct
    26521 aattaccgcg accttactcc cgagcaagaa cgagaattat tagacaatca ttttaaccgt
    26581 gttactgaca catgtattcc cgaagaagaa gctttcagaa gattacaaaa acatattcat
    26641 cagatatgga aagatgccga caataaaggc gagcgttatt ttcctcatga gtacatgtat
    26701 aaaatgcttt tgagtggtgt gttgaaacaa tactatgaga ttgatcctaa aaactcatgg
    26761 atgcttgcag cagcacaaaa aaatattcca attgtagttc ccggctggga agacagcact
    26821 atgggaaata tttttgcatc atacgtcatt aaaaatgaaa tgaaagcaac aaccatgaaa
    26881 agcggtattg aatatatggt ttggcttagc gactggtatc gtcagaattc aggcggtaag
    26941 ggcgttggat tttttcagat tggtggtggt atagcaggtg actttccgat ttgtgttgta
    27001 cccatgatgt atcaggattt ggaatggcat gatgtacctt tctggagcta tttctgtcag
    27061 attagcgaca gtacaacaag ttatggttct tatagcggtg ccgtgccaaa cgaaaaaatt
    27121 acatggggca aattagatat tgacacccca aaatttattg tagaaagtga tgcttctatt
    27181 gttgcaccgc tgatgtttgc ctggatatta gagtggtaat aatatgttat gaatgcagcc
    27241 gttaaataca ccctggcagg cattgccgca gttatattag gatttgtaag cttaggtatt
    27301 attcatcctg aaattgaata tgagtcgagg gtgcagatta gcgcaccggt aacaaaagca
    27361 ttcgatattt ttattgatac ttcaatgatg aaacactgga tgccgggatt cgtttcattt
    27421 aaaccattag agggtaaaat gcttacaaaa ggttctaaac aattattggt cttgaacctc
    27481 aacaacgaac aaattgagat tactgaaacg gttgaagatt acagctatcc gaaattttat
    27541 aaatttcatg cgcaaaatac attactgaat tcaaccaaca caataatttt tgaaggcgat
    27601 tcaattcatt gtagtttgac tgtaaaaaca aaagcaagtg gtaataattt tatgttacgt
    27661 tcaatgtttg tatttactga acccatcttt aaacaacaac aggacagcac atatacgtta
    27721 ctaaaaagaa ctattgagtc acatttagat aatcacaaca tcaatcaata attgtaatta
    27781 aattttattt taaaaaatca ttaaattatc agtcagacat gaatagtaga attttaaact
    27841 ttctcttgct attactgatt tcaacctctg taaacgcgca atcagataca gaactgaaat
    27901 caaacctaaa acaacacatc agcatattag ccagtgataa atttgaagga cgtgaaccgg
    27961 gaaccaaggg tgaacgttta tcttatgaat acattacaca acaatttaag aatattggtt
    28021 tgcaatcatt aggaagtaat ggttatttac aatcatttga gttttctgcc gggacaaaaa
    28081 atggagttaa aaacaaattg accatagccg gaaaaaattt aaaatcagat tccgaatatt
    28141 atgtgttacc gtattctgca cttcaggcaa ccgcagaggg caaatcaata aaaattggca
    28201 gtgggttgtt atcaaaagat ttgggcagaa acgattatga ggggttaact gttttgaaag
    28261 gcaggatagc agtgatggat tatgctactc cggagggaaa taatccgcat agtaagtggg
    28321 ctgaggcatc agacttacgt aagagaatag aaattgccgt atcaaaaggc attagtggat
    28381 tgattattta taattcaaat gattcaataa aaaatcctga aaaaaactcg gaaataaaaa
    28441 tcacaccatt tgcaattcct gttgtattta taaaatcaaa tgatgccaca ttactaaaag
    28501 ataatcagca ggtaactata gcaacagata ttgaaaaaat aatgcactcc ggtcataatg
    28561 taatcggata cattgataac cgtgcagaaa atacaattgt gattggagca cattatgatc
    28621 acttggggta tggcggacat gaatcactct acagaggtaa gcctgaaata cataatggtg
    28681 ctgacgataa tgcaagcgga acatcaacat taattgagct ggcaagaaaa ttaaattcat
    28741 catcattaaa aaaatacaac tacttgttta ttgctttttc aggagaagaa aaaggactcc
    28801 tgggttcaaa ctattttgta aaacatccaa caattgattt gaaaaaagta aattatatgt
    28861 tgaatatgga tatggttggc agactgaaac ctgaagaaca tattttactc ataaacggtg
    28921 ccggcacttc tccttcatgg gatagtttat taaacaacat taaagtagat tctctcagaa
    28981 taaaaactac agcttcagga gttggaccaa gtgaccacac ttcattttat ctggaaggaa
    29041 ttcctgtact gcattttttc agtggcacac atagcgatta tcataaaccc agtgatgacg
    29101 agcctttgat aaattacaat ggtatggttt caatttttca ctttatgtac gacctgattg
    29161 aacaaaccaa caatttacca aagcttactt ttacaaaaac caatgattca agtaatgaag
    29221 atgcgccacg ttttaaagta acattaggtg ttgttcctga ctatgctttt gaaggtagcg
    29281 gtatgcgcat tgacggagtt acagaaggaa aacctgctgc aattgccgga tttttaaaag
    29341 gcgatgtggt aatacagatg ggtgaagaaa aagttgctga tatgatgagc tacatgaaag
    29401 cattaagcaa attcaaaaaa ggtgatactg taaaagtaaa agtacaacga ggaaaagatg
    29461 ttgtagaaaa agaagtaacc ttctgataaa ttattaaaga gcatgacaca acgaaccatt
    29521 cactttgaag aaaaacaata tttgggactg aatcactaca gtctgcttag aagtatggta
    29581 ttggcactct tttgttttgt ggcttactat gttacagaaa accgtgaaaa taatgcagag
    29641 ttactcttct ttggtggtgt agcgtttatg gtaatttcat tactttctat gtttatcaaa
    29701 cacctgcaca ctagggttta tgattcatgt attgagttag atggactctg gacaatgcgt
    29761 aaagtaaaaa ttgatctcag tacaattgtt gatgttcaaa aaatcccgta tagcaactac
    29821 attatgaata ctcctgtata taatctgcac agaaatggca gtatttattt ttatacatca
    29881 ggccgttctg caataaaaat tacggacaag gatggtatga actatattat cggcacacaa
    29941 aagcaggaag agtttttacg aattcttaac gaccttatca aaaaataaat gcccgataaa
    30001 aatcgggcat ttacacattg ttttacaact gcattactct ttaggtttgt ctttcggctt
    30061 ttccatttct gttgccttgt gtgaatcctt ttgaggtgca tcttttttta cttctttatc
    30121 agcaggtgct gactcatgat tggcaggtgc tgcctgaatt gttgcaggcg catctttctt
    30181 aacttctttt tgaggagttg tggcctgctc actctttaca ggttgagcct gatcttctgc
    30241 ctttttttct gtttgggcat tggcgcaata tgttgctgcc actaccgcta atgtaagaac
    30301 tgtttttttc attgttattt gatttaaaga atttacaaca aacataaaca aaaatcctaa
    30361 atctgcaact tatccattgg ctcggatagc ttctacagga tcgagctgtg atgctgaata
    30421 tgcagggatg aatcctgaaa ccaaccctat taaagctgaa ataaacaatc caagcataat
    30481 attatgtcct gtaaggctta gtggataatc aaaggcatgg gacacaaaca tggctaaaaa
    30541 ataaataatg ctcaagccta tcaaccctcc tatcagacaa agtacaatag cttcaaacaa
    30601 aaactgcatc agaataaaat aattcttggc accaagagat ttctgaatac ctatctgact
    30661 ggtacgttcc ttcactgaaa caaacataat gtttgcaata ccaaagccac ccaccaaaat
    30721 agaaaagcct ccgataatag ctcctgccat attcagcgcg ctgaaaatat tggtgatgtt
    30781 attgctcaac aaacttgact gattgagtgc aaaattatcg tcctcaatag ggcgtagtct
    30841 tcttatgctt cgcataatgg attttaattc atcattagca aactgaatgg gaacaccttc
    30901 tttgaccttt gctttgatga aaggatctat tctgtcgctg cgaatattaa cgaaattacg
    30961 tccgaaattt aatggcagaa ccaccgtatt atccatctct gtttcaagca gacttttacc
    31021 ttcttctttg aaaacaccaa tcacttcaac tttcctaccc ataacttcaa aaactttccc
    31081 taaagcatta cttgcacttc caaaaagccc gttagcaaca ttactgccta taatgccaac
    31141 agccctaccg gagttagact ccatctcagt aagataacga ccggctgaaa gttcgaaaga
    31201 ttttaccttg tcaaaatcat gcgacactag ggctacagtt acattttctg cactattgtt
    31261 caggtactta actgttttac cattcaaata tgcttcgaaa gtaaaagcct caatactctg
    31321 agagcgagca gccaactgat gcatttcttt tacagaaggt aacggacgct gccaatattt
    31381 ccaccaaggg taatcgcctc caaaagccca aggccatttt tgaacataaa tagtcttgtc
    31441 tcccaacgac tctacactgg tgcgtacgtt tttttccatg gcatcaacca ccgtaaaaac
    31501 actgataatg gcaaaaatac ctatggagat tcccaataga gataacactg ttctgagcat
    31561 atttgccctc aaagcatgca gtgcaaatgc tatgctttca gctgacaatt tcagaaaaat
    31621 ccttattcgg ttcattgcaa caaaagtata aagttgccat gatttgctct gaatttattt
    31681 tcagtttaac aaattttaat tgattcactt acctattttt gcaaactctc catcaattta
    31741 aagaattccc ggaagtgaag attaaaagtg cgcttatttc tgtctataat aaaaatggct
    31801 ttgaaccact tatcaaggct ctgcatcaac aacaggtaac tttgtttgca actggaggaa
    31861 cttttgattt tatacaatca ttgaaaattc ctattactgc tgtcgaagat gtgacagatt
    31921 atccatccat attcggtgga agagtaaaaa cattacaccc taaaattttt ggtggaatat
    31981 taaatcgcag aaacgatgca aatgatgtga tggaagttga aaaatacaac atcccatgca
    32041 tagatttggt tgtagttgat ttatatcctt ttgaagccac agttgcatcc ggtgctgatg
    32101 aaagtgccat cattgagaaa attgacattg gtggtgtatc actaattcgt gccgcagcaa
    32161 aaaattttaa tgatgtatct atcattgcct ctgcagatga tgtaacagca tttctgcaaa
    32221 cttacaatca gggcaatggt gaagttaatt tatcattcag aaaaaaactt gcacaaaaag
    32281 catttgccat aaccagtcat tacgactctg ccatttttaa ttatttctat agtaagaatg
    32341 aacaatcaga gccggttttg aatttacaat gcaacaattc agttaccttg cgttatggcg
    32401 aaaatccaca tcagcgtggt tattatttcg gtaacctgaa tgaaacattt gtacaattag
    32461 gaggcaagga actttcctat aacaacatgc ttgattgcga tgcagcaatt aatcttattg
    32521 cagaattcag caatgaaaaa ggtgtgactg cagcaattat caaacacaac aatgcctgcg
    32581 gaattgctac aagagaaaaa gcaacagatg catggcaggc agcattagca ggtgatcctg
    32641 tttctgcatt tggtggaata attgttttta atcagcatat agatgaatca ctcgctaaag
    32701 aaatcaatgc tattttcttt gaagttataa ttgcaccatc gtttgcagaa gaagcagtaa
    32761 aagttttttc agaaaagaaa aacagaatta tacttcaatt aaaaaaacag cttgttgcca
    32821 agcaaagtat taagtctgct ttaggcggat atttaatgca ggaaacagac agtgaagcat
    32881 tgagcacaga tggatggaat aacattactt tgcgaaagcc aacaactaca gagacagagg
    32941 atatgctttt tgctgtgaga gttatccgta atttaaaaag caatgctatt accttggtta
    33001 agaataagca gttgattgga agtggtgccg ggcaaacctc tcgtgttgat gcattgaagc
    33061 aagccattgc aaaggcagta aattttggat ttaacctgaa aaacagtgtt atggcaagtg
    33121 atgcattctt tccgtttccg gattgtgtag agatagccaa aactgctggt attgaagctg
    33181 ttgtacagcc gggtggaagt attaaagata aagattctat tgaatattgt aacaaaaata
    33241 acatgactat ggtattaaca ggtaaaaggc atttcaaaca ttaagtctgt tggcagacaa
    33301 agtgttaaaa atgtctgcaa caacagaaaa aatgaaaaaa aagcctacgc ctgaagttct
    33361 cattgtcgaa gaaactataa aggaaaacac taacaataaa agaaaaatgg gactatttga
    33421 ttttctgacc caggagatag ccatagacct tggcacggct aacacgctaa tcattcataa
    33481 cgataaagtt gtggttgatg agccaagtat tgttgctatt gaccgtatga caggtaaggt
    33541 acttgctgtt ggtaagcagg ctatgatgat gcatggaaaa acacatgaaa atattaaaac
    33601 catccgtcca ttaaaagatg gtgtaattgc cgattttgat gctgcagaaa atatgattcg
    33661 cggaatgatt aaaatgatca accccgggag aagatttttt cagccatcgc ttaaaatggt
    33721 tatctgtatt ccatcgggaa ttacagaagt agagaaacga gcagtgcgtg acagtgcaga
    33781 acatgccgga gcaaaagaag tttatcttat tcacgaaccc atggcagcag ccattggtat
    33841 tggcattgat gtagaagagc caatgggaaa tatgattatt gacattggtg gtggcaccag
    33901 tgaaattgca gttattgctc ttggaggaat tgtttgcgac caatctattc gtgttgccgg
    33961 tgatggtttt acgatggaca tttgggatta tatgcgcaga caacacaata tacttattgg
    34021 tgagcgtagt gctgaaatga ttaaaattga agtaggctct gcattgcctg agttagacaa
    34081 ccctccacac gactacgcag ttcatggtcg tgatctgatg accggtattc caaaagaaat
    34141 tacagttacc tactcagaaa ttgcacatgc acttgataaa agtatcagca aaattgaaga
    34201 agccatttta aaagcacttg aaatgacacc tccggaatta tcagcagata tttaccgtac
    34261 aggaatttac cttacaggtg gtggtgcttt gctgcgcgga ttggataaac gtattggaat
    34321 gaaaacaaaa cttcccgtac acgttgccga agacccactt cgtgcagtcg ttcgcggtac
    34381 aggaattgca cttaaaaata ttgacaggta taaattcctg attccataaa catattgtaa
    34441 actcaaataa aaagaggtta gctttataaa aagctaacct ctttttattt gcctggaact
    34501 ttacttcttt cttcaacaat tctattcgtt tgaattttat aaagcaattg actacatact
    34561 gtctttaaaa aatctacgtg ttcagtataa tgaaactgaa aagttattct caaaaaataa
    34621 tcttactaaa taaaagaaca agaatggttt agctaactac tttgatgttt aaactttcaa
    34681 caatcatttt cttttatgac acaagtcata cttaaaaatg ctttactgaa ataatcttgt
    34741 catcgaaaag taaaatttcg attaataatc aattaaaaac caaaaattat atggaagaac
    34801 aagagaataa aaagaaggct tccggacttc aggtagtcat ggatgatgtc atgttacttc
    34861 tttttcttgg agtaacaatc tatgcaatct tttacatcat ctggggaata atggagattg
    34921 ccacagtgcc taatattcct gaatcagtta aacaatctat acttaagtaa aattctaaaa
    34981 caatctattt atgacattaa tagcacctac tgaagggtgg tttaaaaaga aagtttcaaa
    35041 agacgaacgc atgtggataa tcatatcgtt tgtggtttgt atttttcttt ttgcatggat
    35101 ggtactgtgg cacgttttcg gtcgtcagaa tccatcaaac accacatata aaactaaccc
    35161 tattgaattt gcccaactgc atgatgagta tgtaaaaaga aacatggtgg gcatggacaa
    35221 tgggatacct attgtaaaac cggagcccgg cagcgatgtc attcttatgg cacaaatgtg
    35281 gcgatggtca ccggtactta ttttaaagaa agatgagtgg tataacattc acatttcttc
    35341 aaaagattta gtacacggtt tttcattaca accttcaaac atgaactttc aggtatatcc
    35401 tgattatgat tatgtcttga aatttaaacc aaccgttgcc ggtgaagcca aagtattgtg
    35461 taatgaattc tgcggcatag gtcaccacac catgattgga aaaattcttg taattgagaa
    35521 tgatgctgat ctggcaaaat acggttacaa caacctgagt gcaccaccgg cacgcacagg
    35581 caaaggaact gctgaagaag acatttccgg atggagcgaa gaacaactta ttaaaggtgg
    35641 tgagatggtg tataatctga aaggctgcat ggcttgtcac aaaacagatg gcacttccga
    35701 aattggtcct gctttcaaca gcatttatgg aagcaaggtt aaagtaaaag aagatggcaa
    35761 tgcaaaagaa attgttattg atgatgatta tattactaca tctatcagag agccacaaaa
    35821 acaaattgtt gtaggatttg aaaaaacacc aatggctcaa atgaacctta cagacaatga
    35881 aattaaacaa gtaatctcct acattaaatc aattaaataa aatctaaagc ttatgttcag
    35941 aatatgcgat ataaccggat ttaaagtaga tctgcaatcg gaaaaattta tcagattaaa
    36001 tgctgttatg gcagtagttt cattgctctt agcaattaca gctgctatat tgcttgtgtt
    36061 tacacgatac caacctgtac atcttttaga tgctgaatgg tactacagat taacaacctt
    36121 tcatggtttg aatgctctta tttattggat tgtatttttc gaaattgcag gtttgtattt
    36181 cggatccaca gtagtattga atcagaaatt ctgctcacca acattaggat gggtaacttt
    36241 tttactcatg gtaggcggta tggttctttc caacgtaatg gtattgatgg gaaaagcaga
    36301 tgtaatgtta acctcttatg tcccactaaa agcacatcca ctctattacc ttggtataat
    36361 tctatttgcc gttggtgctt taataggtgt tggtttattt tttgccaata ttgttaatgc
    36421 aaaaagagat ggaaatgttg gtgaaacttt acccctcttt acttatggtt taatgacagc
    36481 tgcaattctt gctgttatta ccttagcatc aggagctatc atttacattc cgacctttct
    36541 ttggtcttta ggattaattg actccgtaga tcctgccgtt tacaaactca tttggtgggg
    36601 acttggtcac tcttcgcagc agatcaatgt ggctgccatg gtcagcatct ggtacttagt
    36661 taccttcctt gcgcttggcg gaacatcaat caatgaaaga gtttcacgaa cagcattctt
    36721 cctctacatt ctctttatca gtcttgcatc cgcacatcac ttgttaacag atcctgcaac
    36781 atcatcagca tggaaagtat ggaacacttc ttatgcaatg tatcttgctg tgttagcatc
    36841 aatgattcat gcttttgccg ttccaagttc tattgaatcc gcacaacgca gatgggggtt
    36901 caacaaaggg ctgtttgaat ggctttctaa agcaccatgg tcaaacccgg cttttgcagc
    36961 atgtgttctg gcctgtattg gttttggatt tattggcggt acaaccggtg taattttcgg
    37021 tatggaacaa acaaacatta ttgttcacaa caccattgca attcccggtc actttaaagg
    37081 cactgtagtt attggtacta cactcacttt tatgggtatc acctactacc tcattcctct
    37141 tattttcaga aggaaaattg ttgcattcaa actggcacaa ttgcaaccgt ggatgttttt
    37201 ctttggcgtt gccatcctat ctgtatcaat gattgcctta ggtcagttag gtgttccacg
    37261 cagacattgg gattcaacat tcagcggagg tcctttcact catgagttta atccatcagt
    37321 agatttcttc tggactttaa tgatgcttgg tggtacttta gccttcttgg cagctctcat
    37381 ctgggttttg caggttgttg tttccgtatt cttcggacca aaagtaaacg ggcctgaaga
    37441 catgcagcta ccactttcgc caatggctcc tgccgctaaa gaacataaag gttatgaagc
    37501 acccggaaca ttaattctca cactcttatt ccttgccaca tttgcagtat tgttctttgt
    37561 taactggaaa tggcttgcgg caacatggtg ggtgaaataa tttatttatc tcttttctaa
    37621 ttattggttc gtgattaata ataaaatgca acttacaggc ttagtgatat tactaagcct
    37681 gtttttttct ttatcctctt tcgatattcc cgatgagaaa aaatttattg gtcgcgaggt
    37741 aaagaatatt cctattcttg attcaaaggg aaacaacctt cagctttttg attatcccac
    37801 aggcaaaccc attgtgttgt caccggttta cacccgttgc ccaatggctt gttcgcttat
    37861 cagcaacgga ttaaaaacct ccatcgaaaa attaggtact ttaggaaagg attttacagt
    37921 catttcattt tcgtttgact caactgatac caaagaagat ttgagtggat ttgaagcacg
    37981 ctggaaaatg gatggtgtta actggaaaac ggtcacctcc aacgcagcag gcattggcga
    38041 actattaagg tcaattgatt atcattatga atatgatgaa aaaagtaagg agtatttaca
    38101 tccaaactta gttgttgtca ttacacccag tggtcgaatt tcaagataca tctatggcgt
    38161 tgagcccaaa gctattgacc ttgaacttgc catcatggaa gcacaagttg aaaaaacttc
    38221 tttcaacaat ttttttaaaa cactttacat acgctgtttt acctttaacc ctgcaacaaa
    38281 atcttatcac ctcgactgga agtttatttc gcaaacttct gtcggaatga tgttcatcat
    38341 tattgtaggc tattttctca ttaaaagttt atactccaat aacactgaag acagtgcaga
    38401 attatgatta ataataaatc aagccattgg ctttttcaac gatttgccgg catcttcagc
    38461 cgaatttatt cttctaagtt aaacccatta taccaccttg gcgacattgc aatactgatg
    38521 tttattgttg catgcatcag tggagtttat ctttttttgt tttataatat tgatcctgct
    38581 cacgcctacg aatcagtaga aaaaatttct gccaatccgt ttaatgctgt catcagaagt
    38641 attcatcgtt acagcagtga cttattggtt atttttattg tactccattt tcttcaaacc
    38701 ttattgacag gaaaattcaa acgtattctt tcttgggtaa gtggaattat ttctttgatg
    38761 gtagtgctca ttattggtgt aacaggtttt attcttgtat gggacgaaaa agggaaatta
    38821 gtcggctacc ttactgcaaa attcttttct gcaattccac tcttcgaccc ttcaattgca
    38881 ggttcattta tttcaaacaa tcttgatgtt attggtgggt tttttaaagt ttcagtattc
    38941 ggacatattt tcttttcaat tttcctggta atcgttctat ggctgcacgt aatgcgtatt
    39001 gcaaaaccaa aattattttc accacgcatc atctggattt atagcatgat tgcattggtt
    39061 ttagtttgtt tggtttttcc ggttaagagt gacccttcag cacaagagcg atttattcca
    39121 tacaatacaa cattcgattg gtactacttc ttcggttact catttatgaa agttgtttct
    39181 gttcctatga actgggtaat tatgctggtt tccggagccg gattagcatt ggttccactt
    39241 atatttaaaa agaaaaacaa acctgccaca attgatttag ataaatgtga tgcctgtaat
    39301 atttgttctg aagattgccc atatggtgcc attgatatgc tcatttataa aggtgaacgt
    39361 aaggcaattt tagatcctga caaatgtatc agttgcggaa tttgtcttgc ttcttgtaaa
    39421 gagcatgcca taacaatgcc gggcattcct gaacacaatg tattgccact aaatctggca
    39481 cagaaaagtc aactgagtat tgtttcatgc tcgcagtttg atgatgtaaa aattcctgat
    39541 ggaattacgc ataaaaagaa gtcgctgcct tgcctgggag atttacacat gcatgaagtt
    39601 gaacaaatgt taaacgaaga atcagaagga gttttactgt taggctgcga agattgctac
    39661 tatcgctatg gcagaaatct ggctgttgcc agaataaaca gaaaacgtcc accgggttta
    39721 gcaaaaaaat tatcacgcga cagaattcaa ctcatcacta caaatcacta ttctgcaaaa
    39781 gcaataagag aatttaccga ctcgcttgca tctgcaaaac ccaatgatgg aaaagcaacc
    39841 ataaaagtgt tggattattt taaaaccaat catattgcag cagcattgat actttgtgct
    39901 ttttttctat taatgccttt tttcagcaat acaaaactct cattctttga taaagatgaa
    39961 aaattgctag tattgaattt taaatacata tcatcaccaa cagaatttca agaacaaaac
    40021 accaatcaac aacgacaaat gcagtcacta aaacctattg taaaaaggcg aagcccgatt
    40081 atggttgagg tggcaggcag tgatggaaat atacttctca aaaaagaatt ttctccgcgt
    40141 ggtttaagaa aagatatttc aatttttgtt tatagtgaaa taccaactac cgattcgttg
    40201 gtaaatatta aagttacaga aactgcattt cctgacaagg tagtaagtct gcaaaacgtt
    40261 gcattatcaa caatagatgg aacaatcatt tcttttgctg acggaaaatt gaagcaactt
    40321 aaataagtga accttcaaag tatttttcat taaaagaaat acatagaata ctcctatagt
    40381 agttcataaa attctttttt ctgaagttgc attacttttg ctccaaatat tttatttatg
    40441 tggagaaatt taacaactac ttttattatt agtctgttta ctgtaaacag tctgactgca
    40501 tcaaaactac aacccggtta taatccaaaa gaatacatgg agttgttgag ttttcagctt
    40561 atgcaattcc ataagcagga ctcactatat cctgactcaa tgcattatca caactttaaa
    40621 gaagtatata aatcaccgga gatgggctta ctgaatcaat ggtcgttgtg gatgggtgct
    40681 gactctgttg cagtaatcag tattagagga acaatagcaa acagcacaag ctggctggca
    40741 aacttttatg caggtatgat acccgctaaa ggtcagctga ttttagctcc cggtgatacg
    40801 tttaattatc attttgctga tgattctaaa gcggcagtgc atacaggttg gacattggcc
    40861 actgcatacc ttatacgcga tatactgcca cacattgact catgttataa aaaaggtatt
    40921 accaatttta ttgtcagtgg acatagtcag ggtggcgcca tagcattttt agttacttct
    40981 tgtctttatg actatcaaaa aaacaaccaa atagcttcag acataagatt taaaacctat
    41041 tgcagtgcag caccaaaacc cggaaattta tatttcgctt atgaatatga aaacatgacc
    41101 cgcaatggat gggcttttaa tgttgtaaat tctgctgatt gggttccgga aacaccaata
    41161 accattcaaa ctacaaaaga cattaacaat accaatcctt tggtaaatgc aaaagctggt
    41221 atcagaaaac aaaaatttcc ggccaactta tttgttatgc acatatttaa ccgtcttcaa
    41281 aaaacaccat ataaggccat gaggcaatac agaaaatatt taggtggttt tgcaggaaaa
    41341 ttagttcgga aaaacttagt aaattttcaa tcgcctattt atctcaacag cagcgattat
    41401 gttcgtacag gaacgaccat agttcttctt gccgacaaaa attactttga tttatttccg
    41461 gatagcaaaa ccaaaatatt tgttcatcat ttttatgaac cctatttata tctggcagct
    41521 aaaaggcagt aaaatttttg tttcaacatt atataaaaaa agcgggagga gcaatttgct
    41581 cctcccgctt tttacttcct acaacctctt tgaagttgta agttttaaat tattcaacca
    41641 ctactctaac ctgattgttg ctgccttcag tttgtacatt cagcatgtac gctcctttag
    41701 ctactttgct caggtcaagt tgcaatatgt tttgtcctgc tgcattagca tgtgattgtg
    41761 tcattactac acggccactc agatcaagca ctgttacctg tgctgtgcct ttgtcagctg
    41821 tgtattctac attcaacaca cctgctgttg ggtttggata tacattcagt ttgcttgcag
    41881 aagccatttc gccttcttta caacctattg ttactgcaaa ggcttttgaa cctgcaccac
    41941 aactgttagt tgcagtcaca actactgaac cgtttcctgt tccccaatcc aaagtaagtg
    42001 cattagagtt tcctccgcct gcaacataac ttgctgttgg tgctgatgga tatgtccacg
    42061 aaattgtata tgatcctgtt gtattactca tatcagcagt aaactctata ccttgtgtat
    42121 tggcacacca gcttgaaggt actgcagtta atgcaatcgg tgagtttggt gttcctttta
    42181 cagaaacaca acgtgatgca ctggtaccac aactgttgct tgctgttaca caaacctgtc
    42241 ctgttgtcag tgagccatac tgtacactta ctgtgctgtt accatttccg ataacagttc
    42301 cgggagctgt ccagttgtat gtaattcctg aaaccggagc tacgctgtag ctaacgctct
    42361 gtccgcaaac accattggta ataccggtga tggcaggagg aacagatgga agaccgggtg
    42421 ctacagtttt acattttatt ggtgaggtaa caccgcaaat gctcgttaca ctaacacaaa
    42481 tgtttcctac actgttgtat gaaggactaa atgtagcatt gatgctgtta gttgttgaac
    42541 tacctgttgc acctgctggt agcgtccaat tgtaacttgc tgcacctgtt gatgcaggaa
    42601 cactgaatgt caatgtctgg ttaggacatg ccgtaaatga tccgctgata acattcaatg
    42661 gtgatgctga tgagccaatt acaagacact tcgatggtga attgaaacat ggtgtctgcg
    42721 cacttacaca aagagtacca cctgtccatg aaggaccaaa gtttactgtt acacttgtgc
    42781 cattaccact tacagttgca tcgcctgaaa ctgtccatag ataggatgtt gctccgtcaa
    42841 cagcaactgt actgtacgta cctgagctgt ttgcacatgt tgtagttgct ccgcttactg
    42901 ctgcaggagt acttactata ccacgcaacc actgactctt acgtaatgag ctaccacaac
    42961 cgttaccggc ctgaacacct atgcggtatc ctgatccatt tggtgctcca aacacaacag
    43021 ttactgatgg tgtagttgaa atatagggtg atggattacc attgaatgtt gttccaacag
    43081 ggccatccca tgtatattgc gttgcattag ccactgccgc aacacttatg gttgctgtgg
    43141 taccattaca agcataagca ggcattccta aaattggaac atttactgac tgtgcaggag
    43201 gtgcagtact ggtataagtt aatgaacctg aaatctgcgc ctgacaagca ttgccatctg
    43261 ccacataaat agtgctcatt cctgtcggaa gattattcat gattccatct acattaaagt
    43321 ttataaaatc agaagtaaaa ctatatggag ataaaccgcc cgaagctgat acgtaaataa
    43381 taccatcact gccaccaata catcctacat tcttcagtga gtctatgctt aatgttggat
    43441 tatcattaac actcagactt gtgctctcac ttgccgaaca accatattga ttggttacaa
    43501 caactgtata agttccactg tatagaacac cgggattgat gatagttgga ttttgtagtg
    43561 tgctgttaaa accattaggt cctgtccatg aataagtgtt tccacttgac tgtcccatgg
    43621 ctatgttgtc tcctgttaag gagatatcat tacccaaaca aacatcacca cctgagcttg
    43681 caattggcgt aggtgatggt gataaactaa atgaccatac agtacatgaa ctgccaccac
    43741 caagtatctg tggcactacc atccagtaat atacaggtgc tccacctgat acatctgcgc
    43801 tgaaagataa tcccccctga tttgctgata ctaatgttgt tggaggattg ttggcatcga
    43861 aatacacatc atatcctgta gcaccggaca atgaaggcca gcttaacaga acagtattgg
    43921 taattacatc agcacatgca ctgcctgcat ttgcaggtga tgatggcgct gatatacata
    43981 caggaggtgt aatgtttatg ctgtaatctt cgtactcacc ttgtccgctg ttggtacatg
    44041 ggtttgttgg attactaggt gtaaaacttg ttaaaatacg catgcgtcta actcctgtag
    44101 ttgcatttaa tggaatagta aagctgcctg taattggtga gtacagataa ctattagatg
    44161 taacaatctg ttctgttgcc tgaaaagtac catcggcatt aaaatcaatc caaatagcgt
    44221 gaccaaagtt acttgatccg gctattgtca ttgtaaaatt gatagttgag cctggttcaa
    44281 attcacctga aactgttgcg ctatgatcat cataacctgt tgaactgcca ccactacttt
    44341 gattaatatt agtagttcca ccggttgtag taaagttact gatgtagtaa gttaaaccac
    44401 caccggttgt tggtgtacaa taacatgcag aaggtaaatt ttgagtaacc gtaattggag
    44461 tagaagttgc gtttaatcca ctgtaagagc agtaaactat gacctgatac tgcgtagtaa
    44521 ccgattggct ggttatagca tagatattat taatgcctgc tgaaccaaga ttattccatg
    44581 ttattccgcc atctgttgaa ttctgaaact gataagttaa accatcaacg cctgttggac
    44641 ctgctgttaa attaaaacca atgcctgcac aaatatttgc cggaccactg attgttgctg
    44701 cgggtgtgcc tgtacatgat ggaagtgcta caacttcaaa actgtaatct tcatactcac
    44761 cattaccact gatattacat ggatctgttg gaccactgta agaccagcta gccaatacac
    44821 gcatacgttt agtacctgct gttgttccat taggtataat gaagctacct gttaaaggtg
    44881 atgaaagaaa acttgaactg gttactaatt gttccgttgc ttggaaaaca ccatcttcgt
    44941 tcaagtctat ccaaatggca cgtccataag taccacttgt aacagagata ctataattta
    45001 ttgtagcacc cggatttgct gatgcagatg cactaccact gaaattctga taggggcctc
    45061 cacctacact gctattgtta atgttggtaa taccaccggt tgtggtaaag ttggtgatgt
    45121 aatacgtaaa tccattaact gtagttggtg tacaataaca tgctgttggt gaattttgtg
    45181 ttactgttac agcagttgat gtattagata aaccgctgtt tgtacaggta acaataacct
    45241 gatacatggt tgttactgtt tggttgctta caatgtagtt attaaaaaca tcagcaattc
    45301 ccatattatt ccaggttaaa ccaccatcag tagagttctg aaactgataa gtaaatcctg
    45361 ctaaacctgt tggacctgca gtaagtgtaa aaggaacgcc tgcacaaaca ttagcagggg
    45421 caccaccaat agttgcagat ggtgttcctg agcaacctac cggtgtttga atatcaactg
    45481 tataatcttc tgtctcacct gaaccaaaac cttgggtaca agcatcagta gccaacatgt
    45541 caactgtacg ttgacggata cgcattccgg tatttccatc taatgcagtc accggaatag
    45601 tgattgtaac actatttgtt aatgctcctg ttgaagtact tgaaacctct gtatattcac
    45661 tggcatcaaa agttccgctt tggtcataat caatccaaac acctgtccac acccctgttg
    45721 aagttgttgt tctggtaaaa gtatatgatg aaccttttaa aacagttgtt gttgctgcct
    45781 gataagaata agcaggtaaa gcacatcctg ccccggctgt attattgctg atagtattaa
    45841 aatcaacctg agtaatacat ggactcgatg aggtatgaag gtttgtagta caatagcaat
    45901 tataaaatgg actcattgta acctgaacag gtgttgaagt agctgacatt ccactatttg
    45961 tacaagtaac tacacactga tagtatgttg cagcagtttg tgttgttgtg taagtagcat
    46021 tggttcctcc ggtgcttgtc catgttatac cgtctggtga cgattgccaa tcataagtaa
    46081 ttccaatatc attaatagtg tttgacattg ataatgtaaa tgattgggca ctacaagcag
    46141 atggtaaatt tgactgtgta tttccgggtg caggtgtacc actgcatgag gcaacctgtg
    46201 caagagaaat attatcaatt gctgccggtg gttgtgtacc aatacttcca tcatttttcc
    46261 attcaaaaac caaacgcata gtttgacctg caaaggagct aaggtctaat gtattattgg
    46321 tataggtttg ccatgcactt tgaacattca tgtttgatcc tatctgaatt ctgccggctc
    46381 ctgctgttat ttgagtacct gctgttggat tataggatgt tggtactaac caaaccctta
    46441 aatagtcaca acaagattct ccattggcta tccaattaaa gctaaagttt gtcaaggtag
    46501 tgccgttagg tatagcaata tctctgaaag cttgtactac acttaaactt gaaattgtgt
    46561 atgcattact tgtaccatta tcattagaaa cataaattgc atttgcagga tttccagtag
    46621 ccgagccata agcccattga ttggtttgtg agccatttac aaaggcaaaa tcattacttc
    46681 caaaactatt gctatatggc aatgtagcag gtatttgacc tgtcataaat gaagcacttg
    46741 aagcccaagt actataacta ctaccaccac aataacttct tacatgaaga taataagttg
    46801 tagatggtgt taaaccactc acgcttgttg atgtacctgc aaatgcagta cctgagccgg
    46861 gaggtgtggc acttgttgta acggcatatt cataaccgtt tgatggtaca gggcttgccg
    46921 cagtccaact gatgttagca gttgttgctg ttgttggcgt accaactaat gctgttggag
    46981 caacacaact cggtgtattt tctacattaa aatcatcaaa actcaaatac catgggccac
    47041 ctgttgcatt tactcttatt gcaaaataat aagttcctgt tgctggtgca actaaagttc
    47101 ttgtttgttt tgcataagtt tttgtcgttg ttgtacctga agtaacaaat gaagtaccca
    47161 tttgtgtggc tcctgttcct gtttgtgcag tattgtaaaa cacatcacct gtccatccac
    47221 tgtagttatc acctgcccac cagaaagaaa aatcaacact ttggcctgct gttaatgaaa
    47281 atccgggagt ccagataaat tcgttggttg cactataagc atctcttaaa aactgagcgc
    47341 ctgtgcgggc atcagcatcg tttgaagagc tactattatt tgtagttatc caatcaccgg
    47401 aagaaggatc ttcataccag caagtacttg gatatgccgg aataacggca ctttcaaaac
    47461 cctctgtcca aggtagtgtt gcaatagcat tacatggcgt attgaaagaa gcactcgtta
    47521 cccatgcact gtaatcacta ccaccgcaat aacttctaac atgcagataa taagtggtag
    47581 ccggagttaa gccactaaca cttgttgaag taccggcaaa agcagtacct gaaacaggag
    47641 gcacagcact tgttgtaaca gcatattcat aaccgtttga gggtacagga cttgctgctg
    47701 tccaactgat gttggctgta gttgctgtag ttggtgaacc aaccggtgca gttggtgcaa
    47761 cacaagatgc gactgtagta aatgtacctg ctgcaacata accattgccg gtgcctgcta
    47821 gattaggaat acgagaataa atagcattag ttgaaccatt tgaagcttgc accctaactt
    47881 ggtatgtagt tgaagatgtt aagcctgtta atgttacact atttgttcca acaggattac
    47941 cactccagtt tgtccatgaa catgaaccaa ctgtacgata ttgaacattg tattgcgtag
    48001 cattagccgg tgctgtccag tttaatgttg cattattggt tgttatgccg ctaactgcag
    48061 catgtgtacg tacaggctca gggtctgttg ctacaggttt tgtccatgta taagtaagac
    48121 cgctattagg cttaatggta gcatttgcac tgttaaaata cattgtactg gtattagcat
    48181 ttcctgttac agcattactc cagttgcaag tatttggcga accaacgtta tcaatcatta
    48241 aattattaac atctgttgcc cagttagatg caactgtacc atttgttttc caaccaactt
    48301 gaggatatgt actgcctgtt aaagcagtca ttgtaccgta aacaaacttt atttctcctg
    48361 ttaccgtatt caaacgtatt tgaaagttta aagactctcc agtttgagcg taacgaatgt
    48421 aattaaccca ttcaaaaatc agctcatttc caatggtttg ccaataagct cctgttccgc
    48481 tctgtgaatt caaatctcta ccaaaaggag caaatattac agctccattt gttattgctg
    48541 tacttaatgg tgcatatgtt ccagttgtag ttggtacgct ggcaccatat aatgccaacc
    48601 agccattgga gcacattcgt gcattggtgt aagtagtacc atttacagta aaccctgtta
    48661 gtcctgtaat atttactgcc atatcatcac caataatagc aacaggagca ccagagccac
    48721 taatagaact atatgttcct gagctaatgg ttaacggata cttaattgct aattgcgcct
    48781 gtgcgcttgt tgtccatagc attgcagaca ttatgcaaca ccatagaaag aataaacacg
    48841 ttcgtgttcg atttttggtt ttttctacca tgataaattt gattttagtt aaacatttat
    48901 ttattaaaaa ttttttgcga ctattctatt cttcattcac cagcccgcca gttaaaaacg
    48961 cattaaaaaa gaccaagagg cctcgtagaa aagcatctaa atactaattc ggaatttgaa
    49021 atcggcctca atattattca ttaagtttta attaaacaag cctttgctgg tattttgata
    49081 ctagtatgac aatcattaaa acattatcac atcattttca ataaattata ttttaaaaac
    49141 tgaaaatatt acttcaaaat gattgtaatc attcttcaat taaaatgaaa aacttaaaga
    49201 ttccagtaac ccattacaat tagtccaaca agtacaatag acagtaattc gaaccaccat
    49261 acttttcgaa ttgcaataaa gtagtaactc aaaaacagac aaacaggtgg gataagtaga
    49321 atgaaacgtg atgcactttg ttgtacagga atcaatatta tgagcaacag aaaaataagc
    49381 aataacataa agcataactg aaatttccgc atgcggataa cattcttact gtaattagca
    49441 tatagtttca gaaacgataa aaacaaaagg attgccatta ctaatgcagg aagtataaaa
    49501 cgccatgaaa gcgattgcag tctgataaca ggaagccatg aatctgtatt tacttctgta
    49561 tttcgcaaat gcagtttgtc tgccacaaaa agaattgtga acaacagtat tgcaggagtt
    49621 aaaagtccta atgcagaagc aacccactcg cgccagttaa aaggacgtag tatggcaata
    49681 cataagtaca taaagactac caacaatgcc attggtagat aacacaatat catcagcgaa
    49741 aaaacaagac ctgtatcaaa tgcacgggta acaggacgct ctgatttata taatgtaaaa
    49801 attttccata gcgcaacagg catcaatgtg tttactaaaa gaaaaggact aagtgccaag
    49861 aaaccgggta taagtccata gaggatgatg aatataatga caggaatgtt gctgcttttg
    49921 tataaaacct caaaccggtt gatgagtgca ttaaataaaa aagcctgaat ggtaatgagt
    49981 acaaagatca atgaaggtac agcccatttt attcctgcaa tgttactgtc aagtaataag
    50041 gcatacaaca caccatgatt catgtctctg ataactacaa ccccttgagt ataaatgttg
    50101 aatagtacta atgccagtgt tatcaccggc actaaaaaca gtggtggtgt ttgcgaagat
    50161 ttaaacaacc tgacgatcaa catttttttc agcaactttg tggctgcgaa tatctgtatt
    50221 tgaattttat tatttttgca aaaaattatt gaatatgtta aactcaatct ggcacggtat
    50281 agcagcattt tttgaattta ttttcagctt ggcaaaaccc attggaaaat ctgtagatat
    50341 agtttttatg gtattaattg ccattggatt gttatattgg ctcatctacg aacaaagagt
    50401 tatgggtgga aaaacccgca actatcttgc tgataaagtg aaggaaaaga ataaataacc
    50461 atttgacatt tacttcatag aaaagaattt taaggcagcc tttggctgcc tttttttatg
    50521 tttatcagta agtaattagc attttcaagc ttccttacca tcaattttat acctttgcat
    50581 tccgaaattt tttcattttg cttaaatgtt gaaaacaaca atggttgttg caccacgtac
    50641 ctttactgcc agacttgttg actatggaca gttgtttaaa ctaaggctgg cttcgttagt
    50701 agttctctct gctgcattag cttttcttat aggttcaaca acgatagatt acactaagtt
    50761 gtggcttctt gttttaggtg gctttttagt tacgggttct tcaaatgcat tgaatcagat
    50821 ttacgaaaga cgcaccgata aaattatgaa gcgcaccaga atgcgtccgc ttcctgacag
    50881 cagaatgtcg cttacagaat cgcttgttgt aactatcgca gcaggtttat taggattgtt
    50941 tatcctcacc tactacatta atctttacag cggaatactt ggcttgattg ctttagtaag
    51001 ttatgctttt gcttacacac cattaaaagg tgttacacca ttatcagttt ttgttggagc
    51061 gtttccgggt gctattccta ccctgcttgg ttgggtagcc gcaaccggtc agttcagtta
    51121 tcagggatgg atactttttt ctattcaatt catgtggcag tttccacact tttgggctat
    51181 tgcctggaaa ctttatgacg actatatgag tgcaggcttt caattgctgc cttcgggtgg
    51241 taaaaattct gcttctgcat ttcaaacaac aatatatact tttacactta taccaatcgg
    51301 tctgctgcca tactacagtg gcatgagtgg aattgtatct gcagtagtca ttacccttgc
    51361 aggtatttta ttttttatgc aaagtgttta tttattaaaa acatgttcgg acattgcagc
    51421 aaaaaggctg atgttcggtt catttatata tctccccgtt gtattaattg ccatattact
    51481 tgataaaatt taattacaat gcaaacagtc accacagaac aggataataa ttacacaggc
    51541 attcccacag ctaaaatatt attgtggata gccatggcca gtatggttat gctatttgcc
    51601 ggtttgacaa gtgcctatat cgttcgtaaa aacgaaggca actggataga gtttagttta
    51661 ccaaagatgt ttaccatcag cactattacc ataattatta gcagttttat aattcaatat
    51721 gctatttatg ctattaaaag aaatctgctg aatcaggtaa aaattgcagt cataaccact
    51781 ctcgggctgg gtattgcttt tgtctttatg cagttttatg cctggacagt tatggtaaac
    51841 aacggcatcc atttagtagg taatccatca ggttctttta tatatgtatt gtcaggaatg
    51901 cacattctgc accttgcagg aggaataatt tccatgattg ttatttcagt aaaatctatt
    51961 cttgaaagat attctcccaa aaactattta ggacttcagt taggtgccat tttctggcat
    52021 tttcttgata tcctatgggt ttatctcttc gtatttgtga ccttcatagc ctgaaaggct
    52081 atcttttgct ccaatctgtc aatgaattat ggttaaatgg ccaaaaattt tacaataaat
    52141 tgatgaattg gaaaaaaaat taaatatttg catcctgtta aattaatttc aagactccta
    52201 acttaatatg tcaacagcaa ctgaagcaaa atcaaaatcc atctggggtg gtggaatttc
    52261 accattcaat gtaagctggg gaaaaatgtt tatgtggttt ttcctggtat ctgatgcttt
    52321 tacattctca accctcctgg tggcttatgg taccatgcgc catcgtttta cagatatatg
    52381 gcctgcaccg ggtgatgtgt ttacgcactt cccttttata cacgaacatg tacctttagc
    52441 ttatgtaggt ttaatgacct ttattcttat catgagttcg gtaacaatgg tacttgcagt
    52501 agatgcagga catcagcgca accataaaag tgttgccatg tggatgggat taaccattgt
    52561 tggcggtttg atgttcttag gcagtcaggc atgggagtgg tatcacttta ttcatggttc
    52621 aaaagaagga acttatattc ttgctgatgg tcgttttgca cgcacagacg attatgaacc
    52681 cggaaaatta ttcttctggc acaaccatca gaaaactgtt gttgaaggtg ccgaagcaga
    52741 aaaacttaaa gcatctgcag tgcgcaacat taaaggtgca aacatgactg agaatgaata
    52801 tggcaacacc cccttatttg ccaacttctt tttctttatt acagggtttc acggttttca
    52861 cgttttcagt ggtgtagtca tcaacataat aatttttatt aatgtgctca atggcgttta
    52921 caaaaggcgc ggtcattatg agcaggttga aaaatcgggg ctttattggc actttgtaga
    52981 tttggtttgg gtattcgtat tcaccttctt ctatcttgta taactaatct ttaagaaaaa
    53041 tagaatcatg agtcataacg aacataatga gcataagcca cacggttatt acaccggtga
    53101 tatacacaat cagtttgtag aagagcatta cgaccctacg gctaaaaaaa ttatctggcg
    53161 tacattctgg atattgcttg ttgcaacaat tgttgaagta gcaatagcct ttattccaat
    53221 gcataaagat ttgttgcaat ggatatttgt tgcattaaca atttttaagt cagcatatat
    53281 agtaggtgtt tttatgcacc ttggaaaaga aaagaagagt cttcagtaca ccattctctt
    53341 accatttttt ctgattgttt acatgattgc aatttgtctt attgaaggtg tatatctgtt
    53401 ccatatagga taagcttttt tatcttgaaa aagtttaagg cagcagcggt ttttttaata
    53461 cttctgatgc ctttattttt ttttattctg atgaaagaag ggaaacataa tttccgatat
    53521 cttgaatatt atggcccaaa gcatctggct acaaacggag ttgacaccat ttatcattcc
    53581 atatctgatt tctcgttaaa aaatacagaa ggaaatactg tttcaccaag caacaccgaa
    53641 ccctcttact gggtatttca gttttttaat gcttcttgtc aggaaccgtg caaagaagtt
    53701 tttaccaata tgtatgatgt ggcacgcgat tttgtaactg cacctaaagt gaaatttgtt
    53761 tccatcacga cacagccatt taacgacagt ttacctgtaa tgaaacatgt tatgaaaatg
    53821 aacagcattc atcctccgca atggcagtta tgtacaggcg attcattggg aatagattct
    53881 ttaatgcgca catcattttt cctgaaagga aacaccaatg cagcacttaa ctctgtattt
    53941 ttggttgaca gtaagttgca tcttcgcggc atctatgacg gtgccgattt attagagatg
    54001 aaacgattga aagatgatat taaaattctt agttacgaaa caagacaaaa atcaaagtaa
    54061 gatgaaacag atattaatga atgaaaagct tactttcaga gtagtagcaa ttatttcagt
    54121 tgttgtatta ggaatggtta tgctactaag ccagaaagtc cttccacgtc ctgaaacgcc
    54181 tccggcattt acgaagtacc ttcccggttt aaatgcgttt gtaaacggaa cttgttttgt
    54241 cctgttgcta atttcatttt attttatcaa aaagaaacgc atagacattc ataaaaaaat
    54301 taacctgctt acatttttat tgagtgctgt ttttattgta tcttattttc tatatcatta
    54361 tttagcagaa gaaaccaaat atcccgttga ccatccgttg cgcggacttt atctgttcat
    54421 tttagcatcg cacattattc ttgctgccat agtgatgcca ttggtactgt tatcgtttta
    54481 ttatggttta aaaatggatg tgcgtaaaca tcgtaagctt acacgttttt ctttccctat
    54541 ctggttgtat gttacattaa caggtgtgat tgtttatctg atgatatcac cctattattc
    54601 gtttaaccaa taaaattttc tgtttacttt tttattagtt ggtctagtga accaataatt
    54661 acattcacct gcttaataag ctcagacact ttaactgaca attcaaaaga atattcattg
    54721 tctttagcct tgcgctcaac ctgatctatt aataacccaa cagaattgtt gttgaatatt
    54781 ttcagactag attttatctt atgcgcagcc ttatacagtt tcttcatatc cttgtcttca
    54841 atagcctgca acatttcact gagtaattca ggaaattgta cctgaagcat ttttatcaat
    54901 gaagctgttt cttttttatc tcctgccatt aaagccaaaa ggttgtcata aatttcattg
    54961 acattataag ttgattcata ttcaactgca tcttttgatt gatcattttt caatggaaga
    55021 tgttgtgtta tgagatcaaa cagttgctca ggcatgtagg gtttcgttaa aaaacctgac
    55081 atacctgcag cctttgcctt ctctttctct gcactcgatt cataagccgt caatgcaagg
    55141 ataggtattg tatttatggg atcaggtagt ttacttcgta tatgccctgc cagttggtag
    55201 ccatccattc cgggcatctg aacgtctgta attaccatac taaaattacc ctttctaaga
    55261 agagtaagtg cctcatcgcc atctgctgca agttcaactt tatagccttt tttcagcaac
    55321 attgttttgg caagaaactg atttatttta ttgtcttcta ccaataaaat agtcacatct
    55381 tctgtagccg caggaggttc acttactata ttctttctgt ctgacttaac agccttataa
    55441 ggtagctcaa tgacaaagtt acactttgaa ctttcaagtt ttactgaaat ttttcctccg
    55501 caagcaacaa tcattcctct ggcatttatc agtgacaacc ctaacccgta tccattcaat
    55561 ccattctgcg aaaactgcgg attgttgagt tcaaaaaaat tgtcgggatg cgggaaactc
    55621 tttttattta caatgttgaa ttttacattg ataaaaattt tgttctgcga atgatataat
    55681 gcaagttcaa gtttatcgtt acttgcaaaa gaaccaccaa agaccaaggt attaaataca
    55741 acttttttaa gaataccttc atcaagcaac acttcatcac aagctgcaaa atcagtatca
    55801 gattcaactt tttttccggg gaatttcaat aaaaatttgt ccaggcattc tttaatcgta
    55861 tcgtttaaaa taaaaggatg ctccaccgga ctaacctgtc ctgtatttaa acgtgcatag
    55921 tctaatattt cgtcaacaat atttgataat tgaaatgctg atgacaaaat agtctgaaca
    55981 tactttctgt tttcgtctgt tgttacagtt tccgtcaaca aatcggccat gccaataatt
    56041 ccattaagcg gagttctcaa ctcatgacta acattagcaa gaaatgtatc ttttagtaaa
    56101 agtgattctt ctgctttgct cttctctcgc tgtatctctt ccaggtcata cttcagttgc
    56161 aagcttttag ttctatggct aacatcttcg ctctgtattt cacgacagag tttatagtac
    56221 aacttatgat gtgctaacga tttttcgaaa ttattcaatg cagcataggc atcagataaa
    56281 aacaaattaa ccagatgtaa tggctgttta atgttaagtg attgacagat ttcatctgcc
    56341 tcaagcaaaa tttttatagc ttcatcatga cgatgcatct gattataaag attaccaaga
    56401 ctttttagta agtcggcttt tacacgtaca ttgcctgcaa ggttactgtt atcaatagct
    56461 tgaaaaaaat agtcgagcga tttctcaaat ttattttgct tggtaaacaa aattgctagg
    56521 ctacctaatg tctttatttt taattcagga ctacgaagtt tttccgcaat cttcaaactt
    56581 gtatcaagat aattgaaagc aatatccaat tcatcagccc agttataaca atttccaata
    56641 tagaaattag ttattgaaag ctgctcttta ttatcaagct cttcaaaaat ttttaatgct
    56701 ttctcatgat ggcgaatagc ctcattataa ccaccgagta atttatgggt ctcaccaatc
    56761 tgatttaaca ccaaaccaag cttttgtttg tcttttgctg aagtataggc tgaggcaata
    56821 ttcagtaaat gctcaagggc actatcgtaa atagaaattg ctgtataaat ttctgcggcc
    56881 agctcattaa aaggaatctg attcttcagg ctatcactgt taacttttaa ctcaagacat
    56941 tgattgatta tttttatggc catcaactcc tctttcacct tgtgataagc caatgccaac
    57001 tgcagtccac actctgactt gagcttttca tcatcagcat caatatgatt catggcttcg
    57061 ctgagaagaa taatagcttt agcggtattt accgtcatta atgcctcggc ctgctcgcgc
    57121 agatgactga ttttctgtga aatttcgtta ctcaattttt tctgttttca ggagcaaaag
    57181 taagcataaa atggcagggt tgccactata aatatataaa aatcattatt ctacagctac
    57241 accgtttatc attacccgca taacctgaat gtagcgcata ctacctgcag cgggactgta
    57301 aaccgagttt cttttatctt tcaggtcgtc actgattttt gttgtggtca tttcctcgcc
    57361 tacggcatct tcagtaatgg ttaacttgcc tgccttgata taattattca gtttgccgga
    57421 atcataaacc ctccagaagt taattacact gctgattctt cgttttgaga ggtttttatt
    57481 gtagccactt tctgccaaag gacttgcaga acctcgtaca attatttcaa tcttatttcc
    57541 tgcatcaagc tgcttcagta ctaagtcgct gaaacggtca aggtcaagat agtttttctt
    57601 aacaacctga ttaaagaatg tagtaatttc tgcagcagct ttattacgtt gcgattcatc
    57661 agttaaaaca tcaacataat tctctctgta ctttttttca tttgtaagat atgcattgta
    57721 caagtcaaca taatttgatt ttgttgttgt caacattgtt tttggattgg gttgatcgtt
    57781 ttcgaaatac aacttcattg gtaacaatgg ttctatctgt ttgatgactt ctatagttat
    57841 agaatctttt gtaatgtctg aagcaatagg ttcaatagca atcaaagtat ctttcttaac
    57901 ttgtttaacg gtatctaaat aaacaccatc ataaatatca taacaacaag tgcgtgcttt
    57961 aatgtagagt gatcccggac gatttgatga gagtaaaaag ttattattgc gactgctata
    58021 gtacaaatcg ttatagcttg tattgattgg atagtgtaaa ttctgaatag tttgctcctg
    58081 gctatccggt ttataactgt aaatatcaaa accaccaagt cctttatgac catcacttgc
    58141 aaaatataag gttgaattca ggttgtcata aaatggagtg atgtcatcat caacagtatt
    58201 cacaggtgca ccaacattaa caggatttcc aaaaacaaaa tcaggagtaa tagaacattt
    58261 ccagatgtct gccttaccca aaccaccggg acggtcgctt acaaaataaa gcacatagcc
    58321 tgtcaattca tcatttccaa ttgcaggcat tgtagctgta tatcctttaa aattaatgct
    58381 gtcattgagt ggtttcgggt cagaccaatt gccatctttc tgtgtactgt aatataacgt
    58441 acatatcaat ttatctcttt cacgttgaca ttgcgtatat actaacaatt taccattacc
    58501 actgacagcc gaattacatg aatgcaatcc gggccgattg agttgtgtgc tgtaattatc
    58561 aggacgactc catgtattgc gttcattatt gctgacaagc agtttagata tatacttatt
    58621 ttttttctgt gtctttttgt ctttattttc aaatcgcagc gatgagtaca acaaggttgt
    58681 actgtctaaa tatgacggag caaaatcaga ataaacagaa tttatttcag tgcctgagtt
    58741 ttgaattgta acctttgctg tatctttcaa caactgttta atattttggc aattggctaa
    58801 ttcgcgtttt gctttgtctg taaaataact gttatcacga ccatgcattt gtaaatagtt
    58861 attgtagatt gaagcagctt cctcataata accctgtcgc tgattcatct ctgccagata
    58921 aaatgcaatt aatggaaact gaccttgttt atctaatcca taagccttgt tataatactt
    58981 ctgtgcattt tcataatcat tgtaaagccg gcatgcttct gccagtttcc aggttataac
    59041 taaatctgta ttattgcttg cataagaatt aaaatagaat gcgaatgcag aataataatc
    59101 accttctttc atagccttat cacctgcacg ctcgtaggct gttggagatt gcgcctgcaa
    59161 caactgtaca gcaaataaca agtacattaa cattacaatc tttgcaacaa caatcctcct
    59221 gctgccgaaa aaatatatca ataaacgggg catggtggat tcttctttaa tggttttact
    59281 ttggtgatga tgtaagataa tgctacttcg taagcgcctc tgccgttact tgcccttttc
    59341 aggtcagatg tattgaaatc gtatgcaaaa ccggcatcta aattgccaat acctgctcca
    59401 atacgtgcaa tgacagcatc tttgttacga taaaatactc ctgccgagaa tctgaaattt
    59461 ttaaaattat tctctaatga atgtagttga acatttgcac cgccatttaa ctgtgagaat
    59521 tttccctgac gataaaataa cacttgtggt tgcagagaaa ttttttcgga aagcatcagg
    59581 tttaaaactc caacacctgt gagtcgggta aataattttg ctttatcatt cagaaatgaa
    59641 atatcaggtt gattcaggtg gctggcaccg aaaatcaaat tcaaatcaga attttcacta
    59701 aatgcatatt tataaccggc acctgcattc aaattaaaat tggaatagct actgcgcgct
    59761 gggctttcac ctgtaggcag gttaggatta aagatgtcac catcatattg cgaaccgaaa
    59821 tacagttccg aaaaattcaa accattctgc atccaccccg gctgaatagc taaggtgagc
    59881 tgatgttttt tgttttcatc taaaaaagaa ttccaggcaa cagaaacatc aacaatagta
    59941 gttttataat ctgcatcacc tgtaacgtca ttttcaaata ataaacctgc actccaccat
    60001 gatttttttt catcagtttt tgcaatgttc atatcaacag atgcacctaa tgatttataa
    60061 ggaactgtaa cagatttata ctgtacacga ttaattgcag tcagtctgta atcgccttta
    60121 aagtttccgg tttgtgctgc actctgatgc aatggcgaaa aatcaaactg cgaaaaatgc
    60181 acatcctgcc ctgctgcatt caaacagaac aaacaaattg taaatatgcc cgacaggaga
    60241 tttttcattt cttaccgtat tacggttata ttaccttttt taaagaattt ctgattgtca
    60301 tagcaagtag cctcaacata gtaaacaaac acaccgggtg ttacacgttt gcctctataa
    60361 aatccatccc aaccctgacc cggctgtttg gtttcaaata ctttctctcc ccaacgatcg
    60421 taaatacgaa atagcaactc ttttatttgt ccgccataaa cataaatctc atcattaact
    60481 ctgtcaccat tcgggctaaa tgaattagga atataaattt caggttcttc acaacgatat
    60541 tgtatgacaa caatttttgc agtgtcatta atcttacaac cgaatgcatc tgttatgcta
    60601 aggatatact caatatccac aggaggtgtg ctgacaggat ttgcaatacc gttgctgttt
    60661 aaataagttg gtggctgcca gttgtaaatg taacctgtat tttgagttgc tgataggtta
    60721 acactttgac cacgataaat ggtatcaata gaaggcacaa catctaatgc tgctaacgaa
    60781 tcagaaaaca aaacacttac ggttacagga gaagtagtac aaccttttga gtcagttaca
    60841 gaaacagtat agttgccggc acaaagctga ttgagagaac ctgaatttga accattactc
    60901 cagacaaagt tgtatggtgg tgtgcctcca cctgccaatg ctgtggctgt gccaatacat
    60961 tgaccgctgc aataatcagg agttgcagtt gcagataagg taagttgtgg tggttcagaa
    61021 atatttaaag aaaaatttga agtacatcct gcagaatctg ttacagtacc tgaatagttg
    61081 ccttgacata gattaacaat agtatcattg gattgtcccg gaggattact ccacaatata
    61141 gtgtatggcc cattattgtt tgttactgcg acaaggccgg aaccgttaca ttcgcctttg
    61201 caacgtacat tctgaatggt agttgcagtt gcactcatga attgttgtat gttaatagtt
    61261 ttggtaacgg tatccttaca accgagcgaa cttgtaacaa ctaaggtaac cggataatta
    61321 cccggtgagc cataggtaac ttgcggatta cttacatttg aagttgttcc attaccaaaa
    61381 ttccaattgt aagaaaatga tgcaggtgca gatgataaaa tagtgtcgtt aaataatgct
    61441 actgttgtac atgcctgaac tgtagatgta aaatctgtta tcagttcttt agttaccgtt
    61501 actacaccat gaatggtgtc ggcacagcca ggattaaata aagaataggc aattaatgta
    61561 acgttatacg tccctgtatc gggataagta taaaccggat taaaagcatt agaagtatcg
    61621 ttggtgagat tgggtacacc aaaatcccaa tgatatgaaa tggcaccaat gcttccattt
    61681 tgaaatgaaa ctgtattact gccacaagaa actattggat ttaaaaaagc ggcaactacc
    61741 aatgacggac aaggcactac gtttaactga taatctcttc ttgtctgacc taagtaaaca
    61801 ttgttgcgat attcttttgc acagacacca atcacaaact gacctattgt gtttggagtt
    61861 gcagttagca cacctgttat tgaatcaata ctcagtggtt gaattcccgg tgtaccattg
    61921 agcatattgt tcacagaata aggtggtgca aatattacat tattgtatgg tggtgcagaa
    61981 accggtggaa gtggtgccgg aactatggtg tcgccaccaa ctaagggtgt gcatagtgaa
    62041 taacgaatgg tgtcattatc aggatctgtt gcagaatggt cgaaaacaaa agggaaattt
    62101 agacaaacaa acggtggtgg caaaaattta aaacgagggc tactgttgtt aaaaaaactg
    62161 cttgctctta tttcgccaac ataggtagca ccagtcacac caggtccaat taaattattg
    62221 atggtattat tccggcagca acgctgatag gcaatatagt aaacacctga agaaggtggt
    62281 agattaacat taaaaatata ggtagctcgc ctgtaacaaa tattcacagg tgggataaaa
    62341 catggattgt tgatagttga aggaataaga agagaatcag gtctaacatc aacaaataat
    62401 gcttgctcta agttaaaact ctgatcgtag ataccaattg cagcaggatc atcaaaaggt
    62461 ggtacaccat tggcacagtc acgatagata tacattgtga cctgatagtt tccattacca
    62521 aggtagctgt aaaaaatctc tcctccaaca atatgcgttg cctttgcact gtctgcaaac
    62581 agaaacaaaa aatttattaa taaacagaac ttccaaaagt ggcttttcat ataagatgga
    62641 aaggtaacaa agatactaaa attgctttct atataatgac tacaaaaatt aaaatatcat
    62701 tgtctttaat cggtaatttt tctgttttaa aaaatagaaa cttcaaaaca taaaacttgc
    62761 taaagtggaa tattatttga attcaataat atcaggtttt tttaaatcga aatcaaccaa
    62821 ctggaaatct gtgcttctaa gcggattgac tctgaacaca tcttgccctg aatatggaat
    62881 tgttggataa tatgccaggc ttatctgaaa actactaagt gccagatatt catttttaat
    62941 aatcattcca actccaaaaa cagaatacat acgacttctg ttaaaaccat gaagttggtt
    63001 accgatgaag cctgctgatg caatcagata cggtccgaac ttaaatccca aaaagtcata
    63061 aggcgcatag gtttgtgtct gcagagagat aaaaattcgc ttcgtgccag aagtgcggct
    63121 atcgaaacct ctcatctgat cttcgagcga aacaaattca ttggtgattc tgtcaatacc
    63181 caatgttgca tttgctctca ggaattgcct aagcctccac ttccctattg ccatttgccg
    63241 tgtaaaataa acggtctgta tttcaaatgc tgaatttctg gcattaccgt tataaaaaaa
    63301 tgttccgtag cgaaccaaac tgctcagata accaaaacta aaataatctc cgaatgctgc
    63361 cattgcacct gcataccaat tacctgatga agccctttct ctatagccca ccgttaatcc
    63421 ataggtgcga ccaacaggaa tatcttcttt ctgtccatat ttaaagacat acctttccgt
    63481 aatgtactct cttgaagaaa atccgattcc tgcaagatag gtgttctgat tttttaatag
    63541 acctaacgaa tcaaatcctg ccgaaggaaa agaaagatac tgaacgctgc tgcctcttaa
    63601 tgcagcaatc aatcgtgtgt tgcgctgata caccttatta ccacttaaca aacgcatgga
    63661 ataacccgac caggcatcta taaagttata tttatatcgt tcggagtaat tttctgatgg
    63721 ctcaccatat acggccttta cgaagtgttg ttctaataat attccacctg catattttgt
    63781 gagtggcgaa taaaatggcc tttcaactcc aattgacttc acatcgcctt ctctttcgtc
    63841 caaatggtaa agtatttttg catctgcatg ggttcccagc aaattcggaa cctcataact
    63901 gctttcaatg atgtcggcat ttttattgta ataccaccga taactgttct caaaagtatt
    63961 tcctaagcct gcaaagtttc tttctgttac actgattttt gtattggttt cactcatgcc
    64021 tccgccaatt aaaatactcc agttatccag cacacgtact tcaaggtcta tggtttggtt
    64081 tagctcattt ttatgaggaa taataatagc atcacgtata taaggttgtg cacgaatcaa
    64141 tcgttccgac tctgaaacta acaatgagtc aaactcatca cctgtttcaa taatcattaa
    64201 ttttctgatt tgccggttgc ttgttttatt atgcaaataa tttccggcat tgataactga
    64261 tggctgtttt ttaatggatg aatctgctaa agaataacca aacggatcga atactgtaat
    64321 gctaatgttt ccgactggca gacattgaaa ttccgaaaaa ttaatttctt gtacaacagg
    64381 ttttttctta acctttacgg cttttgcagt aacaggccgg aaaatcattt tgtaaatact
    64441 acgaagtagt ttactcgatt ttgaaacctg ctcaactccc tgataaaaat caacagattt
    64501 attttttgtt gtatctgttt gtgctattgc tgaaccacag aaccaaaata aaatcagaaa
    64561 agaaacaata ctgcggttca ttattttcat tgatacaaaa gtaaattgcg tatgcaatta
    64621 tgatttacga aaataacaat aagccccgaa ctaaaaagta taaaaactat ttcagctttg
    64681 ccagactaac ctttatacgg gcaatagctt ctgtgagcag cttatcactt gtagcataag
    64741 agaaacgaat acattgattg tcaccaaaag cttcacctgt aactaaagca accttggcat
    64801 ccttcagcag atacatacac atatcttcag gattattaat tacagtactg ccatctgttt
    64861 ttccaaaata ataacttatg tccggaaaaa cataaaatgc accttcaggc gtattggttt
    64921 tccatccggg aatatctttc atcaggttca aaaccaagtc gcgtctttta cgaaaagttt
    64981 ctaccatcat atctctgtcg ggtgtgctaa tgcttatagc tgcctttgct gccttttgtg
    65041 caatactact tgctgcagaa gtaaactgtc cctgcatttt atcacatgca gttgccacat
    65101 cctgtggtgc tgccatatag ccaattcgcc atcctgtcat tgcatagccc ttacttacac
    65161 cattgattat gatgacctga ttttttaatt cttcaaatga tgcaatgctg tgatgcttac
    65221 caacaaaatt gatgtactca taaatctcat cactcagcac aaaaatattt tcatgttttg
    65281 caaaaactgc tgcaagtgct tttaactcat catgcgaata aacagtacct gtaggattac
    65341 agggtgatga aaaaataaac atccttgttt ttgatgtaat tgctttttca acctgctgtg
    65401 gcgtaacttt aaaatcagaa tcaattgtag tattcagata aataggtaca cctcctgcta
    65461 attgaataat ctgcgaatag ctaacccaat aaggtgtagg cactaacact tcatcacccg
    65521 gattaacaca acataatact gcattggcaa tacattgctt agctcccgtg ctgacaacta
    65581 tctgtgatgc tttataatcg agattgtttt cgcgttttaa cttctgcgaa atggcttcgc
    65641 gcacatcagc ataacccgaa atgggtggat acttcgtaaa cccgtcatca atagcttttt
    65701 ttgctgcatc gccaatatat tttggtgtat caaaatcggg ttcgcctaaa ctgaggctga
    65761 taatattatg tccctgatcc tgtagctcgc ggctaaggcg tgccatggca atggtttgcg
    65821 attcatgtaa tcgctctata cggtctgcta attttcgcat gtctgatgca ttcaataata
    65881 gggcgcaaag ctaacaatac ttcatcacag aaaataaaac aaaaagcagg taattaacca
    65941 tgctttgtgt attaccggaa agttatttac ttgattacac caagtttttt tccgacttta
    66001 ataaaagcat tcaatgcttt gtcaatatga tggcgttcat gccctgcaga aagctgtact
    66061 cttatgcgtg catttccttt ggcaacaaca ggataaaaga atccggtaac atagatgcct
    66121 tcgtctaata acatagctgc aaactgttgt gctaactgtg cttcgtaaag cataatgggt
    66181 acaataggat gctgaccggg tttaatatca aaacctgctt cggaaatttg tttacggaaa
    66241 tattgtgtgt tgtcccaaag tttatcgcgt agtgttgttg tctgactaag catatcaagt
    66301 acagcaatgg aagcacctac aattgaaggt gccaatgagt tggaaaacaa atagggtctt
    66361 gaacgctgac gcagcatttc aataatttct tttcgtccgg atgtgaatcc acccatagca
    66421 ccacccaatg ccttgcctaa tgttccggtg ataatatcta tgcgtcccat cacaccatga
    66481 tgttcatgtg tgccacggcc tgtctttccc ataaatcccg atgcatgaca ttcgtctatc
    66541 atgattaatg cctgatagcg gtcggccaaa tcacagattt tatctaactg tgcaatggtg
    66601 ccatccatac tgaaaacgcc atcagtaaca atcatacgat gacgtttatt catagtagct
    66661 tttaatttct cttcaagatc tgccatatca ttatgtttgt aacgatgacg gtcggccttg
    66721 cacaatctga caccgtcaat aatagatgca tgattcagtt cgtcagagat aatggcatcc
    66781 tgctcattga gcaaaggctc aaacactcca ccgttagcat cgaaggctgc agcataaagt
    66841 atagtgtctt ctgtttcaag aaatgatgca atctttgatt caagtgtttt atgaatatcc
    66901 tgtgttccac aaataaaccg cacacttgac atgccatagc catgtgagtt gattgcagca
    66961 tgtgcagcct caactacttt tggatgcgat gataaaccga gataattatt ggcacaaaaa
    67021 tttataactt cttttccgtt ggctgtaata tcggcagcct gtggtgtaga aataattctt
    67081 tcttgcttaa acaatcctgc tgacttgatt tcttcaagct ccttttccag cacgggcttt
    67141 aatgtgttgt acatatcatg aaaattttat gcaaagtaat aaaatagaat tcatttgaac
    67201 ctaatgctgc aaatatctaa acgagggtat tttcctactt tagccgcatg agaattgtgc
    67261 gaatagtctt tggtaatgtg tggcggttct ggtttttctt tgaagcggtt gttacctttt
    67321 ttctgttgtt tccgtttttg cttttagcat tgcagggcaa agcaccctat cgtcaggcat
    67381 ttaaactgat gaaattccat gcccgcacta ttgcttttct ttcaggaata aatgtaaaag
    67441 ttatcaggaa atatgaaccg atggagaatg aaacctatat tatctgcccc aatcatattt
    67501 cttatcttga tattgtgctg acatacgtag cttttcctca ctattttcat tttatgggta
    67561 aggcagaatt agagcgcgta cctttcttta atattttttt caagcgaatg aatattggtg
    67621 tgaaccgttc aagccggatg gcatcgcata aagcatatca aagagcttgc aaggatcttt
    67681 cgcaaaatat cagcatctct atttttccgg aggcaacgat acctgactgc aatccacaat
    67741 tagggccaat gaaaaacgga gcatttaaac tagcaataga aaatcagata atgcttttac
    67801 ctgtttgtta tattgacaac tggaaaatat tacccgattg tccaagaaaa attctgggcg
    67861 gcagaccggg cattgcacgt gttattattg acaaacctat tgaagtaaaa ggattgacag
    67921 aagcagatgt ccctgaactt aaaaaacgct atagagaaag aatggacaac atacttcagg
    67981 aatatcagta tgggtatggg aaatagtttt actatttgaa gttttaataa taaaattcag
    68041 acagttgtta agcagacata gtaaatgata aagataattt ccataagtat attatcaatg
    68101 ctgttatttg ccattacaca gccaaatgat ttccttgctg aacaaaagaa atttataaga
    68161 gtaaggactg caatagaaga aaaacaaagt tttattgaag aaaaattaaa agaacataaa
    68221 atcacttctg ataatctgaa catacttatt gttgcctata aagaagacga tttgcttgat
    68281 gtttacgcta aaacaataca agaaacaact tataaaaaaa ttctttcata caatatttgc
    68341 tcccgttccg gacaaccggg accaaaaagg aaacaaggtg atgggcaagt gcctgaagga
    68401 ttttatcaca taaacagatt taatccaaca agtagttttt atttgtcact tggaataaac
    68461 tatccaaacc ttgcagacaa aagaaaaagt aattcaagta atttaggtgg tgatattttt
    68521 attcatggat cgtgtgtaac tattggttgc ttaccaatga ctgacaacta cattaaagaa
    68581 atatatttaa tttcagttct tgcaaaaaac tgcggccaat ctaaaatacc ggtttatatt
    68641 ttcccgttta aaatgtctga ccaaaactta actacttatt gtgccaaata caaagagaac
    68701 aaagggttaa tttcattctg gaacaacttg aaaacgggtt atgataaatt tataaaaact
    68761 caaaaagagt tagcaatagg tgtaaatgag aatggggatt acatatacta aaaaactgtt
    68821 gccaaaatgc agcttaaata attttgcgtt ttaaatttca gaattcaggt tttaggctga
    68881 gataaaaaaa ttgtcatgca acaatgttgc tgcatgacaa ttccgaaaat tatttaattg
    68941 gaatatcagt aaagttaccc gaaaagctgg taacaccgct aacgcctcca cctgcagtgg
    69001 cactaaaggt tccggaaagt ttgctatcct gattttttgt aattgttaca gtacctgctg
    69061 ttgtagccca aaagctgcca tttttaatat aggttagcga atttgttgta cctaaactat
    69121 aaccttgtgc tgtaatgccg gtcaaattaa tttctactac agtgcttccg cctgtttggg
    69181 caatgatagt attgaatgca ccattaaccc atgatgagtc ggctttggtg agtgtagaag
    69241 ttccgttttc accgtaataa aacccggcac tttgtgcagg tggattaggt gaggcattgt
    69301 catcatcctt ctttttgcaa gaagtaaata gcactgccga cactaatgca gcagtcataa
    69361 cataattaaa tagtttcatt tttttgtgtg tttaaattat attgcttgaa acgcaaaatt
    69421 ctcatgctgg ttacgcaata gcaatgggct gttcagccca ttttgaaaaa ttagaaaaaa
    69481 tgaattgtta ataactaaac ctttttcaaa aacctactca atctcatcag atacaaaacc
    69541 tttaacaagt ttctccattg atgtttaaat atccacctta aaaataatat ttttgtaata
    69601 ttatagctaa aactatcgtc actataaaga tgacttaatg aaaaaattag tattctggtt
    69661 tctattactc agcacctact cgactttcgc tcaaaagata tcagttaaag gaaatgttca
    69721 ggataccact gccagagaac cactttcaaa tgctttaatc atggctatta agctcatgga
    69781 ttcaaccttg gttaattata cccgtagtga tgaaaaaggc ttttttaata taaatgcatt
    69841 acctgttgat acttatcagg taattataac acacccaaag tttgccgata tgggtttttt
    69901 tatttttgga gacagtacta atttagaata cgactttgga aaaattattc ttcaacctaa
    69961 aaatatagat ctccatgaag taacgattta tgcttttaaa gatcccgtct attataaggg
    70021 cgacacgtta atttatacgg cagattcatt taaaacaaaa gctaatgcaa atgttgaaga
    70081 tttgttaaag aagttacccg gattatccgt agatgcatcg ggaaaaatta cttctcaggg
    70141 aaagtcaatt gacaaagtct tggtggatgg tgatgagttt tttggaagcg acccaactat
    70201 ggctacaaaa aacttagcag caagttcaat agaatctgtt caggtatatg aaaagaaaag
    70261 tgatgatgca gctaattcaa gtacaggaga
//
LOCUS       DAOOOL010000002        49016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_49_length_49016_cov_115.319663, whole genome shotgun sequence.
ACCESSION   DAOOOL010000002 DAOOOL010000000
VERSION     DAOOOL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 49016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49016
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49_length_49016_cov_115.319663"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3088
                     /locus_tag="PKU82_00300"
     CDS             <1..3088
                     /locus_tag="PKU82_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOZ81343.1"
                     /translation="RKGVEELRNDAEFVRLKNNEFFEEIPVDEVLGKKAESKDATPRR
                     DFLKFLGFGVAAASMAACEAPVRKTIPYLISPENLVPGIPNYYASTFFDGHDYASVVV
                     KTREGRPIKVDGNELSPLTKGGANARVQASVLDLYDSERIKSPIAKGKAASWDTVNKE
                     IKEKLAAANTKQAKIRIVSSTIISPTTKKAIESFTTKYPTAKLVTYDAMSQYGIIKAH
                     QLAFGKAALPNYSFDKADVIVSFGADFLNNWIAPIEYARQYAQTRKLDDGKKTMSKHI
                     QFEAALSLTGSNADNRYKLKASQTGNALLSLYNAIASKAGAATVSAGKTDADAAINST
                     ANDLWNAKGKSLVVCGTNNANDQLVVAAINNILGNYGATISIAKHSNVRQGNDEAMAE
                     LLREMNAGEVGVVIFYNANPLYNYPDTTAFTSALNKAEVKISLSDRNDETAIKCDYIC
                     PDSHYLESWNDAEPVRNMFSLVQPTIQKLFDTKQSQELFLLWSDAPVTDYHEFLMQNW
                     RQGFLATATGMASVPAWEKVLQEGVWNGEVTESEPAFVGNVNDAASAIGARKSGGLDI
                     VVYEKAGIGNGRHANNPWLQELPDPVSKICWDNYFAIAPSMAKEKGLVQGNIIEVKSG
                     NVTLKGPVLLQPGQAKDTIAVAAGYGRTNSGKAGNKVGMNAYPLLTFSEGTFNYTNSG
                     ASLNKTVEADYTLATTQSHHTMMGRAIVKETTLEEYIKDPKAGNEEEKFHSYKGKVSA
                     KELDLWATKDHPGFDRPINAWGMSIDLNSCIGCGACVVACTAENNVPVVGKEEIQRSR
                     EMHWIRIDRYYTSDMDKEKAHEQGVGKLSMYGFMEEPAENPEIVFQPVMCQHCNHAPC
                     ETVCPVAATTHSEDGLNMMAYNRCVGTRYCANNCPYKVRRFNWFKYSDNPQFDYNMNN
                     DYGKMVLNPDVIVRSRGVMEKCSMCVQRIQYGKLEAKKAGHPVKDGAINTACAQTCPT
                     NAITFGDYNDKSSRLVKQSGSERMYHLLEELNVQPSINYLTKVRNTTGKTNNSHKA"
     gene            3126..4511
                     /gene="nrfD"
                     /locus_tag="PKU82_00305"
     CDS             3126..4511
                     /gene="nrfD"
                     /locus_tag="PKU82_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOZ81344.1"
                     /translation="MSNVESPLRPTLIEGDKTLHDITEDVCRPIESKPNKYWWMVFSV
                     ACAALLWWIICVAYTVGTGLGVWGLNKTVGWAWDITNFVWWVGIGHAGTLISAILLLF
                     RQKWRLSINRSAEAMTIFAVICAASFIVAHMGRPWVAYWPLPFANQFGSLWVNFNSPL
                     VWDVFAISTYFSVSLVFWYTGLIPDFAAVRDRAKTKARKFWYSALSMGWVGSAKNWQR
                     FEEVSLVLAGISTPLVLSVHSVVSMDFATALVPGWHTTIFPPYFVAGAIFSGFAMVQT
                     LLIITRKVLNLEEYITIYHIDMMNRIILITGSIVGCAYLTEFFIAWYSGVEFEQYAFI
                     NRATGPYWWAYWSMMTCNVISPQLMWSSKLRRNLFVTFFLSIIVNIGMWFERFVIIVT
                     SLHRDFVPSSWTMFHPTWVEIGLFIGSIGLFFVPFLLFTRYFPVIAMSELKTILKSSG
                     SQSKKGVQHHD"
     gene            4504..5016
                     /locus_tag="PKU82_00310"
     CDS             4504..5016
                     /locus_tag="PKU82_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOZ81345.1"
                     /translation="MTKQLHGIFNDDEVLLNAIKELKSKGIKCTNAYSPFPVHGLDSA
                     LGLKRTRISIASFLYGATGTCLALLMMWYMNIWDWPMDIGGKPSWNLYKNIPAFIPIT
                     FEFTVLCAAHGMVITFYLRSKLLPGVTPNVIHDRMHDDHFVLTVEGKVSDETTIRESL
                     KQVGAVEIRS"
     gene            5030..5635
                     /locus_tag="PKU82_00315"
     CDS             5030..5635
                     /locus_tag="PKU82_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ81346.1"
                     /translation="MKNTHIYFNVIIGGMILLSACGDKQSPGLEYMPDMYRTPAVVAY
                     SPSHITKDSVSALAPVAGTVARGQYVDFIYPNTNEGYEAAGLELKNPFQPTAENMERG
                     KTEFDTYCAHCHGEKGDGKGWLKIKGDPFPVPSYTSAAIKDLPDGKMFFSITYGKNLM
                     GSHAAQVSADDRWKIILYINHLQGDEPGTVVADSSATAQNK"
     gene            5664..6866
                     /locus_tag="PKU82_00320"
     CDS             5664..6866
                     /locus_tag="PKU82_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HOZ81347.1"
                     /translation="MQDNLTYTFSSKLKTLSFILMAIGAISIGISFATNPDQTWANLM
                     HNNFYFLTIALGGLFFYAVQYAAEVGWSVYIIRIPLAMSQYLWISGVIMLILFFAAGH
                     HIWHWTHHELVMETLPDGKPNPDYDEILAGKSAYLNVPFFLIRMVAYFVIWYGFAVLL
                     RKHAMKEDLSGAIEHHNRSRKLSIIFLVLFAVTSSTSAWDFVMSIDSHWFSTLYGWYT
                     FSGIFVSSLGVIALIIVYLRSQGYLHHLNENHLHDVGKFIFAFSIFWTYLWFAQFMLI
                     WYANLPEEVTYFMERQDYYKTLFVTNFLVNFILPFVVLMTRDAKRKLRLLAILGSVIF
                     IGHWIDTYLMVVPGIMHEKWTIGVLEIGTTLGFMGMFIFTVFTALTKLSLLPQKHPML
                     QESEHFTL"
     gene            6908..7933
                     /locus_tag="PKU82_00325"
     CDS             6908..7933
                     /locus_tag="PKU82_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOZ81348.1"
                     /translation="MTLLITISILLGILVLVRLMTIVQLSSELTGSSDSQEEQIRHNN
                     SNGFGFIVFMVLGLGLFIYMTIDYSKHLLPVSASEHGVGLDKLLNINWAIIGVVFFIT
                     QIALFYFSYKYRDNKKHEAYFYPENYKLELIWTIVPTIVLTTLIITGLKQWNGIFSEK
                     ENSMNVQVYGYQFAWITRYSGPDNTLGKSNYKLITDDNPLGLDASDPAVKDDIYTTGN
                     EMHIPLGQELKFQFNARDVIHSAYFPHFRAQMNLVPGMNTFFSFKPTITTAEMRKITG
                     NDKFDYVLLCNKICGVAHYTMKMKVVVDTPADFKAWLKSQKKASEVKAAPPVSALNST
                     VKGSNSI"
     gene            7985..9805
                     /locus_tag="PKU82_00330"
     CDS             7985..9805
                     /locus_tag="PKU82_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ81349.1"
                     /translation="MEVKQTAGVAVEHHDDHTHEHDHHETFLTKYIFSQDHKTIAKQY
                     LITAIIMAFFGMYMSLIFRLQLGFPETKFAFLESLIGKWGKGGRLDPGFYMALVTIHG
                     TIMVFFVLTGGLSGTFSNLLIPLQVGARDMASGFLNMLSFWFFFLSCAVMTYSVYIET
                     GPAAGGWTVYPPLSALPQAMPGSGLGMTLWLVSMVFFIIQSLLGGINYICTVINLRTK
                     GMSMTRLPLTVWAFFITAILGVLTFPVLFAAALLLVFDRSFGTSFYLSDIFIGGVALP
                     QSGGSPVLYEHLFWFLGHPEVYIIILPAFGLTSEVLSTMGRKPIFGYKAMVGSLMAIA
                     FLSCIVWGHHMFVTGMNPFLGAVFTFTTLLVAIPSAVKVFNYLATLWRAKIQLTPAMC
                     FAIGLVSLFISGGLTGIILGDSALDINVHDTYFVVAHFHVVMGASAIFGMLAGVYHWF
                     PRMFGRMMNKTLGYVHFWITLIAVYGVFFPMHFIGLGGVPRRYYSNSAYEGFDVFLNI
                     NTLITVFAFLAAVAQVIFLFNFVYSIFKGKKAPQNPWHSNTLEWTSPAGPGIHGNWNG
                     PVPTVHRWPYDYSKPGADDDYIPQTVPYSQTKNSNLPGEE"
     gene            9989..10420
                     /locus_tag="PKU82_00335"
     CDS             9989..10420
                     /locus_tag="PKU82_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOZ81350.1"
                     /translation="MIALIFNSDNLFAFSDSTKVVVKKPKKEKYYKRSRIAVEDVRNY
                     MDSIGILNSDIVLKQALLETGHFKAKLLMDKNNLFAFRFTKRYMSFINWQASVDYYKK
                     WQDKFYTNQSENYYDFLKRIRYARSPHYIKVLKQVKARTAQ"
     gene            10436..11833
                     /locus_tag="PKU82_00340"
     CDS             10436..11833
                     /locus_tag="PKU82_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOZ81351.1"
                     /translation="MKNSRNNEPIKILFFLTVILSVISIMPKYLTGSKKINLYSDIIH
                     KENKKEKPQSLAIVNDSVFFKNDTVKQVLTDTAKSTATKINYKSGELDLTRFFEALHN
                     HENENGKIRIAWFGDSMIEGDLVTQDFRKLMQKIYGGSGVGFVPVTSPVAQFRQTIRQ
                     TFSDDWEVYSFMKKPKQNYKLGIGGYSFVSSKGSWVDYKASRFYPVYSEVKMICRQCP
                     PKSFILNEDTSLVFPVDSIGSFKEVELIRNNPIKEVKLTFKKPNCEVHGLYFDKGNGV
                     YVDNFSFRGNSGIPLSNIPFGELKSLGAKENYKLIILSYGLNVVAHDVKNYSWYASSF
                     QKTLNYIKEAFPDASILVMSVGDKSYRAEDGYETEPDIPIFVELQKRIAQRSDVAFYN
                     LYEAMGGYNSMVSWAEGNPQLANLDYTHPNFAGGRKIAGMLYDDIIKSYDRYSKTVET
                     MEVKRDTMIKPQAKQ"
     gene            11830..13071
                     /locus_tag="PKU82_00345"
     CDS             11830..13071
                     /locus_tag="PKU82_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOZ81352.1"
                     /translation="MKRIILIFSLLIYCTKCHAQSDSDTTMPVLQYEKFVEYKMSLLK
                     FLKPDAIDIEQKEALKNFYSKLLQSRADGKKVSIVHIGDSHLQAGYFSGMVRRGLQQR
                     YGNAGRGIVFPYKLARSNGPHDYVSFSQNSWKGNRNVITQDSLSTGITGFSVTTNDSS
                     PSVGISLRNNEGIDYRFNRVQVFYKPDSTFMTASDSLAIIETVNPIKLATGINEFRFS
                     DYLSNCKINISSDTTYTLFGLNLLNDSSGVVYHNIGVNGAEYKSFLGQPMFFEQVKEL
                     NADLYILSFGTNEAFAKYFDGTVFIQTVDSFITKLRNVSPTASILITSPTESCKRIRR
                     GVYVPNPNVIAIKNLLQKYCMDNKIAFFDLYECSGGKGSMLRFAKNKMSDARRIHLNR
                     SGYEMQGLMLLQALLKPLSEQ"
     gene            13068..14537
                     /locus_tag="PKU82_00350"
     CDS             13068..14537
                     /locus_tag="PKU82_00350"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOZ81353.1"
                     /translation="MTIDWEKLGHQFLYDKANPLLFNSSFFLFYLLLFLFVFRLLQRT
                     GTLRIVAVTLFSLYFFYKASGVYVLLVLAAAVIDFNLSNFIYNATSDFRRKALLVFSV
                     VINLGLLAYFKYTNLLIDLYNAILTGNVQPIDVYLPIGISFYTFENLSYTIDVYRRQF
                     VPVKKFIDYLFFLSFFPKLMMGPIVRAADFIPQIRKEIALTAHDVSKGLFLILTGLFK
                     KVVISDFIYLNLVQYVFDDPLRHTGMECLFAVYGYALVIYCDFSGYSDMAIGIAKWIG
                     IDININFLAPYQSASITEFWRRWHISLSSWLRDYLYIPLGGNRKGEVRTYINLFITML
                     LGGLWHGASLNFIFWGALHGVALAFHKLWKAAYPSHFSGRLTRIAGIIITFHFVCLCW
                     IFFKCTTFTDSFNMIHQIVFNFYAEGWTLFSANYSEVLLVMLLGFVLHFLPIRWNEII
                     INYTEKWHWLLKASVATAVVIFVIVLKQANPVMPVYLQF"
     gene            14614..16395
                     /locus_tag="PKU82_00355"
     CDS             14614..16395
                     /locus_tag="PKU82_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOZ81354.1"
                     /translation="MKRLFIVISVFFIAACKDSGKQSIKDTSVLLKEITDGYYQERMR
                     YFPLEATANADNRYNDLLPIDISDAYIDTLRQFYHRYSEKLLTINKPELTGQDLISYE
                     VLQYTLNIEEEGLKFPSNLMPVNQFWGMHLTFSQLGSGTSSQPFKTVKDYDNFLQRIS
                     AFVAYQDTSINNMKRGMLQGFVPARILIERTIPQYKSLIANKVEESVFYGPIKNMPDS
                     FSDADKKRLTDAYSNAILNQLNPSFEKMAVFLEKEYLPACLPVAGISNIPGGKEYYNW
                     LANKWTTTTMSPDEIYNLGLKEVERLHSEMDKVKAETGFKGSLKEFFHFIHTDPQFFP
                     FKKDSDVIAAYLAIEDRMKPQLKKLFNHVPKAAFEVRQTEAYRAASASAEYNTAAPDG
                     SRPGVFYVPILDATKYNVVGMEDLFLHEAIPGHHYQISLQQENTSLPEFRRFGWFGAY
                     GEGWALYTESLGKELGLYTDPYQYFGCLSEEMHRAIRLVVDVGIHLKGWTREKAIQYS
                     LDNEMESEADITAEIERYMAIPGQALSYKIGQLKIIELRQKAEEQLKDKFSIQKFHDQ
                     LLSYGCLPLSVLEKSIKLWIKEQKKQG"
     gene            16478..17464
                     /locus_tag="PKU82_00360"
     CDS             16478..17464
                     /locus_tag="PKU82_00360"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOZ81355.1"
                     /translation="MKQLIENFPSQMREAMTIGETFKATSWPHEIKNVLVTGLGGSGI
                     GGTIVSQLCEKEMPIPFMVNKDYFMPAFVNKHSLVVVSSYSGNTEETLHAMDMAIKQG
                     AKVVCVSSGGKTVELAKQHGLDHILIPGGMPPRSCYGYSSTQLFYILHAFKLIGDGFK
                     ASLKNAVDLLDAESANIKNASHKLASELIGKIPVIYSASNNEGVVVRFRQQVQENGKM
                     LCWHHVFPEMTHNELVGWTQPHNDIAVVLFRDKDDFFRTAKRMDICKEIFGRYTPHIY
                     EVWSKGGSPIEKAIYLIHFGDWTSWYLSEIRNIDCTEVKVIDYLKSEMAKLN"
     gene            17489..18118
                     /gene="tmk"
                     /locus_tag="PKU82_00365"
     CDS             17489..18118
                     /gene="tmk"
                     /locus_tag="PKU82_00365"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOZ81356.1"
                     /translation="MKKENLFIAVEGIDGSGKSTQVQLLKANLEEAGYKVYATNEPTN
                     FEAGKLIRSIFNHRVNADQHTIAALFVADRLQHLLDKENGILAMLEKGYTVITDRYYF
                     SSYAYHSAYVEMDWVMSINAKCAALLKPDLNIFVDVDPETAFKRIIATRTSTELYETK
                     ENLRKVYDNYLKAFKLLKNTESIVFVNGKNDVQQVAADIFKTVKNLLHT"
     gene            18181..19815
                     /locus_tag="PKU82_00370"
     CDS             18181..19815
                     /locus_tag="PKU82_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOZ81357.1"
                     /translation="MNKKHPKALPYLFLSEMWERFGFYLMLGIFFLYMTDTQRGGMAM
                     DRKEASDIFGTFIALVYLTPFVGGLIADRVLGYRVSITLGGILMGIGYCGLAIPGMTA
                     FYVSLLLIIIGNGFFKPNISTILGNVYNDPEYKPLKDSGYNIFYMGINIGAFICNFFG
                     SYLRNNFSWGAAFAAAGIGMFVGVIIFWIGNKHYAHADVRKPVKPEDMPVSKILGITL
                     LPALIAGYIGWIIPENIFGSDSTDAFILGALPVAGYYVNILLRSDKEDRRPLAALLAV
                     FAVAIVFWAVFKQNGTALTTWAESYTNREVPQSVEPVAKVLGVTQTVTFSKDSVPLTD
                     SQFRVARDENKKAIKTWDYPLYYKNVPADKLPKEGESLTLISTELGQSINPFWVVILT
                     PVIVAFWAFLKRRNSEPSTTWKIFYGFLITSLSTLVMVAATYACHNGLEKSSMWWLIA
                     SYGVITVGELCLSPMALSLVSKLSPPRLTALMMGGWFLSTSIGNKLSGVLASMWDEYE
                     HKANFFLVNFTLVMITTIIILLMLKWLNKIVKEHGA"
     gene            20027..22546
                     /locus_tag="PKU82_00375"
     CDS             20027..22546
                     /locus_tag="PKU82_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81358.1"
                     /translation="MRLLSTAVILLGCLCAQAQISITNNEMPSAGDTARFTKAVVNPL
                     INFSATGTNHTWSFGNLRNNGQDLKSYQNVSSTGFIYSLFFSNLPFNPNRANVTEPGQ
                     ALPSNPLLTITDPYNFYYRSSSVYKQVGFGAEIANIPMPVAFSQHDIIYNLPLNYNNS
                     DTSHSAWNIGLPGLGYYGLTQTRINHVDGWGTLTTPHGTFNTLRVKTQLYIKDTISVD
                     TLNLNFALNRPKTTQYKWLANNEIIPVLQITTTEILGLEVVTEIIYRDDYNTVSPGSL
                     NAAYCAGSNVSIPYTATGSFNGSGFLQTANSFTAQLSDATGSFANAVNIGSVTSRVSG
                     NINAVIPANTPVGNGYRIRIISSSPALTGGDNGFDLRIENNAVASIVNQGLSAFCSGN
                     SALLQSVNVDSGYTYQWQLNGTDISGATTDNYLASATGNYTLNITNSCGTITSNAAAI
                     NVLPMPTVQVTASGPLTLCLGTTVTLSTLFDSTYSYQWQLNGVDISGATTENYTAASG
                     GDYTVVVSNSCGNVTSSVQTVSVQSAPVAGSLTAASSTICHGDSVQINYSLQSAGITW
                     LHNGNILSGITTNSHFANSAGVYSVVESDLCGSDTSVAVILTADSLPTAVITASGALS
                     FCAGGDVVLTAATGNNYAWQWYMNGTPLATTQLITATQSGNYEVEITNACGSVMSTLV
                     AVTAFPLPVVPTITLSNDSLLATTTVGTYQWFYNGNAITGATNDFYVPSQNGNYSLMV
                     TDANGCSSMSAVYNMTTVGLQTIFKQAHNVFPNPFNDYLTIELPQTENAIILTVEGKQ
                     LWSGTLHQGKNVLNMQQFVSGMYILQLNNENGITNYKLVKR"
     gene            complement(22592..23254)
                     /locus_tag="PKU82_00380"
     CDS             complement(22592..23254)
                     /locus_tag="PKU82_00380"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOZ81359.1"
                     /translation="MKKYIALVSVILILDQCLKFWIKTNMYLGQEYHIAGNWFIIHFT
                     ENNGMAFGMELFGKKFLSLFRIGVMFALAWYVWHLHKTKAHHIYTTAMALIFAGAVGN
                     IIDSIFYGRLFSSSEFGVATFMPDAGGYAGWLHGKVVDMFYFPIIQTHYPQWFPLWGG
                     EEFIFFRPVFNIADSAITAGVLLIILFNRTIFKHIETTPETEQTTVEMRSEELNTANH
                     NS"
     gene            complement(23326..23742)
                     /locus_tag="PKU82_00385"
     CDS             complement(23326..23742)
                     /locus_tag="PKU82_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOZ81360.1"
                     /translation="MLDNRPTKLENSPDKTRYSDAELNEFRELIIQKLTDAKVELNNL
                     QAFLNNSNEHGTDDTASSFKMLEDGSDTLAKEEAGQLAARQRKFIEQLENALVRIENK
                     TYGLCRVTGKLIPKERLRAVPHTTQSIEAKVQQYRD"
     gene            23732..24340
                     /locus_tag="PKU82_00390"
     CDS             23732..24340
                     /locus_tag="PKU82_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81361.1"
                     /translation="MSSIATGFLRIEATGASGRTGSSRNGVVRGGAAGLTIGGLLRLR
                     ELSLGFSTCLIIDVADLLLSSFCDFAVTPGFIILIGFGLTTIAGLTTFALATGFAFAT
                     FFATALLLITLLFATAFLVTVFDLAGLPFATAFLATTFFATGLLLTAAFFTTLTTFLT
                     AVFLFFGVAFFAAAFFGAAFFATGFFFALLAIFKSFLFQLMF"
     gene            complement(24309..27773)
                     /gene="ileS"
                     /locus_tag="PKU82_00395"
     CDS             complement(24309..27773)
                     /gene="ileS"
                     /locus_tag="PKU82_00395"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOZ81362.1"
                     /translation="MSHKPYPEFKQLDLAAIANDVLLKWDNEQTFDASITSRQGAKAY
                     NFYEGPPSANGLPGIHHVMARAIKDIFCRFKTLQGYQVKRKGGWDTHGLPIELSVEKT
                     LGITKEDIGKKISVEDYNKACRKEVMKYKEVWDDLTRKMGYWVDLKNPYITFENGYIE
                     TCWYLLKELYKKDLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKIVKDTTVVAQFRIK
                     KETLPQSLQKDLQNINCDFYFLAWTTTPWTLPSNSALAVGEKIGYVWVKTFNQYTNEP
                     IVVVLAKDLFAKYFSEKNAALSLEDFKPGDKQIPFKVIAECKGSTLTGIAYEQLLPYV
                     KPDGKAFVAVAGDFVSTEDGTGIVHIAPTFGADDFRTAKQQGISAILVTDEGGNPSPL
                     VDKRGRFVKEVTDFAGEYVKEEYLTTNEKEAERQKQGRDKYLSVDERIAIKLKQENKA
                     FKVEKYEHNYPHCWRTDKPVLYYPLDSWFIRTTACKDKMIALNKTINWKPESTGIGRF
                     GNWLENLVDWNLSRSRYWGIPLPIWRSEDEKEEKCIGSIIELRTEIEKAVAAGFMDAH
                     HLEHVDASKSDFDLHRPYVDNIILISDTGKKMYRETDLIDVWFDSGAMPYAQWQLDQE
                     KLSKGLPMPFNEGFNTGYPADFIAEGVDQTRGWFFTLHAIAVMLFDSVAFKNVVSNGL
                     VLDKNGNKMSKRLGNAVDPFETIGKYGPDATRWYMISNAQPWDNLKFNVDGIAEVQRK
                     FFGTLYNTYSFFALYANIDGFVPDEKNKVPVQDRSELDRWILSKLNNLVQRVTDSLND
                     YEPTFAARQIEEFVDEHLSNWYVRLSRRRFWRSEMNTDKQSAYETLFECLMVTAQLMS
                     PVAPFFSDWLYRNLTDSMRSNAIAAKTPLQYTSVHLTYLTKAETKMIDTALEERMDFA
                     QRISSMVLALRKKVNIRVRQPLQKILIPVDSPKQQMAIEQVEKLILTEVNVKEMEFVT
                     DASGLLVKKIKANFKVLGKKLGALMKDAAAAIAQMTQDDIRNLESKGTFDLRINQNIV
                     AISVEDVEISSEDIPGWQVNSNGGLTVALDISISPQLRDEGIARELINRVQNLRKDKG
                     FEVTDKILVKLKNQPSIITAIESNLDYICAEILASSFTWVETLATPVADEIEVEEGVK
                     TLIEIERI"
     gene            27964..28344
                     /locus_tag="PKU82_00400"
     CDS             27964..28344
                     /locus_tag="PKU82_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HOZ81363.1"
                     /translation="MKSLFLSVLFYVLSNFTMAQVASKLASKPDAAKELITVETSCGE
                     CNFKLPGDNCDLAVRINGTAYYVDGVSISEFGHPHDDNGFCVAVRKAEVQGEVVNNRF
                     KATYFKLLPVANASENSNKQDGGK"
     gene            complement(28359..28970)
                     /gene="yihA"
                     /locus_tag="PKU82_00405"
     CDS             complement(28359..28970)
                     /gene="yihA"
                     /locus_tag="PKU82_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOZ81364.1"
                     /translation="MEISSAEFVQSNTDVKKCPEPNKPEYAFIGRSNVGKSSLINMLC
                     NKKGLAKTSSTPGKTQLINHFIINGQWFLVDLPGYGFAKTARTEREKWDGMIRNYMIY
                     RKNLMSVFVLIDIRISPQQKDLDFINWLGEKSIPFSLVFTKADKQTRAKNDFSVKQFC
                     EQLYKTWATLPPYFVTSADTKSGRQEVLDYIDSINQGFKQSGR"
     gene            29042..30013
                     /locus_tag="PKU82_00410"
     CDS             29042..30013
                     /locus_tag="PKU82_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81365.1"
                     /translation="MRNSFYILIFALVVLTSCHNDKSSDNPPCPAMEMHISGDTTVVA
                     GDSIGLMATLFTGATYSWTGPNGFIANTYNPVISESSSLSEGYYYASADINGLCKSNV
                     DSFYVTVICDRPEDVLANYITTIQTNDTIHLFAHSITDSVEYIWTGPAGFTSTQPNPI
                     ITNAIPGNEGVYTVYAHKDHCYSKPDSVVVSFNQCNPSSNTFANTLDSNYFLYSYGSA
                     NLYPNTRSITGTGLNGTSQLRATFYGNNPPTGNYIINPAHCPASGSGALAPGEVCVEF
                     SNASGTYQAVSGTVKLKALNTFDFCDAPFRLQFTTTTLFNGSGKVQF"
     gene            complement(30010..30939)
                     /locus_tag="PKU82_00415"
     CDS             complement(30010..30939)
                     /locus_tag="PKU82_00415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOZ81366.1"
                     /translation="MQIIDPVERHLLEQELNNGRFIRDANNGQNKLYVVTAHDSPNVL
                     QEIGRVRELTFRAAGGGTGKALDLDEFDLIENCYKQLIVWNPADKEIVGGYRFIRCAD
                     AATDPHSQYKLATTELFHFTEKFIKDYFPHTIELGRSFVQPQYQPSAENRKGLFSLDN
                     LWDGLGAIAIDNPDMKYFFGKVTMYLRFNRIARDLILAFMQHYFPDKDKMVYPHHPLP
                     AENDVHDFVASLKNLPYKEGHHNLNKKVRELGENIPPLFNSYMNLSATMKTFGTALNH
                     EFGDVEETGILISIDDIYESKKARHVNTYHPAH"
     gene            31087..31653
                     /locus_tag="PKU82_00420"
     CDS             31087..31653
                     /locus_tag="PKU82_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOZ81367.1"
                     /translation="MKNGLLIYCVLLTLAVAYLFYKVNSCTSNCAPAGVATAMNDGNI
                     VFVNVDTLLEHYDYYISLRESLKKKQDSIDVVLKNKGKELEGEIVNYQKRGASMTDQQ
                     RGAEEERLGHKQQQLVEYKNHLLDMLTEEEAVMQDSLHKHLVGYLKDMNKQTNFKFVF
                     GYQRGNGILLANDSLDITQSVIEGLNKK"
     gene            complement(31661..33160)
                     /locus_tag="PKU82_00425"
     CDS             complement(31661..33160)
                     /locus_tag="PKU82_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81368.1"
                     /translation="MDYRFKSMLAACFFLTATAFAQNKNDALRYSMQQTFGTTARALS
                     MGGAFGALGADFSSLSINPAGIAVYRRSEFTFSPVLQFGNSDATYLGTLTSDNKVNLG
                     VGNLGFVLAFPREKKTSGWMGTSLGFGYNRINSFSDRFAFEGINKNNSINDYFAEISN
                     GISKANLSGEDMYQYDAGLAYNSYLTNPVADSSLIDQYQGALYNGDLKQKGLITRTGS
                     QGEINISFAANYENQVYLGATVGIPYFRYSSTEVYEEYALQNTTKDSLSGKTFKNSKY
                     TQKLSTQGNGVNLKLGVIFKPTDMVRLGLAIHTPTYYEMNDQYSSKIYANFSEGYLEN
                     KSPDGRFSYNLTTPFKVITSVGLVFLDHGLISADYEYLDYSTARLNSNEYSFSDENRD
                     IKASLKNGQILKIGAEYRYKIFSFRAGTVMQSSPYEGGITYKEGNRSAVSYTGGLGIK
                     GGNFFLDLGYAYTKTSGNYAPYTLADGSAPSASINTKINRFMMTFGAKF"
     gene            complement(33189..34832)
                     /locus_tag="PKU82_00430"
     CDS             complement(33189..34832)
                     /locus_tag="PKU82_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81369.1"
                     /translation="MKSIALSAAAIFTLASCTSSQQASNAYSDDVYYSSKANKPAETV
                     QTQQQVQQPQQDVSDQSVSNQGSSTSAENRFDYNDAGTTTQDGNTYVTNNYYSDDDYY
                     DYAYSSRIRRFYRPMWNYGYYDSYYTNSYWYDYNPYDYGVSIYCGYNWWAPSSWWAPS
                     FTYFSWGYTPYNYWWSSPYSYYGYGPYHHHGWNNYSYGYWNGYNNGYWNGYYDGYYNG
                     YGYNPYYFNSYDSYSYNNTYYGPRRSTSGSGSSNAVPPRSIADSYNRALVKNGRNSMP
                     VSQSFANAMSLPTRDTKDAEVIHPRNNVNTNSPVKDSRNVETAPVRNNDAEVIHPRNN
                     VNTNSPVKDSRNVETAPARNNDAEVIHPRNNVNTNSPVKDSRNVETAPVKNSRADQFS
                     TPRNNNEVKDNSTTPAAPPVRNNSRNEVNPEYNSPRNTETVPPRNYESNPRNNNNTPA
                     ESPRRNTESNPRYNTPAESPRRNYESAPRNNRNSETAPRQEAPVRNNPPRQEHTPRQE
                     RATPAPRQERNFSEPSQQRQESRPRQESTPSRNNSRNGR"
     gene            34986..36461
                     /gene="proS"
                     /locus_tag="PKU82_00435"
     CDS             34986..36461
                     /gene="proS"
                     /locus_tag="PKU82_00435"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOZ81370.1"
                     /translation="MAKDFPTREQNYSEWYNQLVIRAGLAENSSVRGCMVIKPYGYAI
                     WERMQQQLDSMFKATGHSNAYFPLFIPKSFFSKEANHVEGFAKECAVVTHYRLKQSDD
                     GKSVVVDPDAKLEEELIVRPTSETIIWNTYRNWIQSYRDLPILLNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATAEEAVEETRKMLDVYAEFAEQWLAIPVVKGVKSANERFA
                     GALDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFTTKQGVQDFVWATSWGVSTR
                     LMGALVMAHSDDDGLVLPPKLAPIQVVIVPIYKNDEQLKLVTDIALKIKKALTARGIA
                     VVYDDDEQAKPGWKFAEYEMKGVPVRLAIGPRDVESGTVELARRDTKEKMTVQITDIE
                     LKIENLLEQIQQNIFKKAVDFRETSTHVADSMDEFIKILDTKGGFVFAHWDGTAETEE
                     KIKEQTKATIRCIPLNNKQENGKCILTGKPSTQRVLFARAY"
     gene            36474..37160
                     /locus_tag="PKU82_00440"
     CDS             36474..37160
                     /locus_tag="PKU82_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81371.1"
                     /translation="MEPVNDKIAELIKTHFPLNCKIVEFASGHFNEIKNYLKKFVDGT
                     ASYYQPPNKRQRIKFYDTGSNQCELQLGDDTLWFHLHPNLFRISPDNPAYRYSYLRDD
                     EYRSRCAVIHVYNFLSDSLAERRSDDYGMLVARIFINYENHFMVEGRKQLGMLFNDIS
                     NDILTEQRLNDVLDQVILFCLQYDLFAQPFDQVPAMSYREISSGSFGSAVASSKQLGF
                     KLKAEDHDTE"
     gene            complement(37220..38587)
                     /locus_tag="PKU82_00445"
     CDS             complement(37220..38587)
                     /locus_tag="PKU82_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81372.1"
                     /translation="MKKIYAFVIVLLIYSFTNSVSAQSQNAIDFDGLDDQVVSVNASS
                     LIANSSQISLSFWVYPRNPVPGFPDFDGLAGFRDNTVADFYVLQYSSTAVEARFRNSA
                     GINFDILSQGLQLNTWQQFALTYDGSYLRLFRDGLITDSVSASGVISSTATSFYMGNL
                     VFSGTNFLLDGQMDEVALWNRALTPQEILCIYKNKVDATSAGLQLYYDMNTGTAGGNN
                     SGILTIPDISNHINGVLQNFSMNGPSSNFVNGIDNATIITDTICSGQTYSFNGQTLTQ
                     AGTYTATLVSSANCDSVVRLTLFSNDTTVNQNQAVLTAVQVGATYQWVDCSNNFSPIP
                     GATNQTFTATANGSYAVIISANGCTDTSSCHSVTTVGINEFSVSNWITFPNPTVSVFN
                     LVTNEIINNAEIIITDISGKQISRMTKDIKYQYAVDVSTLPDGSYIITLKSEKKSKNW
                     RLIKI"
     gene            38859..40481
                     /locus_tag="PKU82_00450"
     CDS             38859..40481
                     /locus_tag="PKU82_00450"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOZ81373.1"
                     /translation="MSAVKYVFVTGGVSSSLGKGIISASLAKLLQARGYRVTIQKLDP
                     YINVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQTVIN
                     KEREGAFLGKTVQVIPHITDEIKRRIKILGETGEYDIVITEIGGTVGDIESLPFVESI
                     RQMIWEMGSNCCVIHLTLLPYLNVTGELKTKPTQHSVKTMLEYGVQPDILVCRSEKHI
                     TADLRRKIALFCNVNVNAVIEAVDASTIYEVPLHMLKEQLDKVVLNKLKLSFKQEPDL
                     TAWKAFLGKLKHPVSEVKIGLIGKYIELKDAYKSIAEAFIHAGVANECKLNIEWIHSE
                     KIEEENVAEKLKGLSGILVAPGFGDRGIEGKIAAIKYARENKIPFLGICLGMQCAVVE
                     FARNVLGLKDAHSSEMNKETKNPVIDLMAKQKEISSKGGTMRLGEYPCALAKGSKVAQ
                     AYGKTKIYERHRHRYEFNSKYQKRFEDAGMIATGINPADNLVEIVEIKNHPWFVGVQF
                     HPEYKSTVENPHPLFVRFVKAASEFAKGAIQI"
     gene            complement(40478..41410)
                     /locus_tag="PKU82_00455"
     CDS             complement(40478..41410)
                     /locus_tag="PKU82_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81374.1"
                     /translation="MNHRFSALLLMLFISIATLSCKKEGSKYDTPVKDIEQFFDETGA
                     PVQQFSFSSSANINQTTAAGNTFMISAGSFTKTDGSAVSGNIDIKVRECVSKRDIILS
                     NLTTQSGNKFLTTGGIFYLAAYQNDVALSLSNGKTINFFLNAVFGNGQMLHRYYSSSL
                     PLENNSSDVSWTADADTQMINLLSSSSQSHYAYVMKNTGWQNAAEPVFSNSSRKKITV
                     DTGTDYETSNTAVFVSVDGSNTICKAKSVTLGVWEVENFPVNLHVTVMALAKVNDNYF
                     FSKSSVIVSTDVSIPLVMNSITLPALKTELEQLP"
     gene            41670..42440
                     /gene="surE"
                     /locus_tag="PKU82_00460"
     CDS             41670..42440
                     /gene="surE"
                     /locus_tag="PKU82_00460"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOZ81375.1"
                     /translation="MSKPTILVTNDDGITASGIRALVEAMREIGHVIVVAPDSPQSGM
                     GHAITIHNPLRLDKVDVFKGVDAWQCSGTPVDCVKLAVNKILHRKPDICVSGINHGSN
                     SSINVIYSGTMSAAMEGAIEGIPSIGFSLVNYKLDADLSAAKHYAGVITKTVLGGGLH
                     HNLLLNVNIPYLQLDEMKGLKICRQAMAKWKEEFDERLDPAQQKYYWLTGKFINPDLG
                     EDTDEWALANGFVSVVPVEFDFTAHHAIPVLNKLQWNV"
     gene            42433..42717
                     /locus_tag="PKU82_00465"
     CDS             42433..42717
                     /locus_tag="PKU82_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81376.1"
                     /translation="MFKKIPDTFHIGALSALALSVLAWLLISRTESYIVTSALKDYLA
                     FPRLYFIVFALQLVFLRIMMINFQKQHFARGWMFSVFIFALFVFYLIKKS"
     gene            42720..43007
                     /locus_tag="PKU82_00470"
     CDS             42720..43007
                     /locus_tag="PKU82_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81377.1"
                     /translation="MFSKDKFITGFIGGIIAPVVGFYLYYIALFRQMDLSGFYNTLVS
                     NNTLAAAISLSLLANLALFFVFDKFGKLNGSRGVIAATFIYGFYIVYLKFF"
     gene            43013..44131
                     /gene="lpxB"
                     /locus_tag="PKU82_00475"
     CDS             43013..44131
                     /gene="lpxB"
                     /locus_tag="PKU82_00475"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOZ81378.1"
                     /translation="MRYYIIAGEASGDLHASNLALQLRKYDTGAIMRGWGGDKMQQAG
                     VEIVKHYRELAFMGFVEVIKNLRTILRNLEICKEDILAFNPDVVILVDYPGFNLRIAE
                     FLKKKNIKVFYYISPQIWAWKQSRVHKIKRDVDKMFVILPFEKDFYKKFDYDVVFTGH
                     PLLDEIEKIKHQPAKALSQSDIDKKIIALLPGSRMQEIKEMLPVMLKAVSVFKNFNPV
                     IAAAPNIPDSFYQSLAGKDIPIVHGRTYDILQQAYAALVTSGTATLETALFKVPEIVC
                     YKGNRFSYLIARQLVKVKYISLVNLILDRMLVKELIQDDLNEKTLTSEMKLLLEDEEY
                     RGNILAGYDELEAKLGMAGASEKTARKMVDLLKGTVDQ"
     gene            44163..44699
                     /locus_tag="PKU82_00480"
     CDS             44163..44699
                     /locus_tag="PKU82_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81379.1"
                     /translation="MKTTNYILVLIATLMLSACQKQEAETDNEPQVAATCDDGIMNQG
                     ETDIDCGGPCPLCQATMNAMVDADTFKAVFNTITSKYANGAFFMSGSSNLGSLTVIYS
                     GPLNIGAVSNAQATYVKSSTSVTYIASNANLTFNAINVTDSTAEGTFSFNAINFNSPT
                     DTIKITNGFFKRISIKNN"
     gene            44958..46295
                     /locus_tag="PKU82_00485"
     CDS             44958..46295
                     /locus_tag="PKU82_00485"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOZ81380.1"
                     /translation="MKKIVVFLVALMLTNIINAQDLKPFKSDKELWGYKDATGKEIIP
                     ARYNFAWSFTEGIARVRNKKMKYGFIDVTGKEVIPIKYEDAKDFYEGLAAVKSDDKCG
                     FIDKTGNVIIPLRYDEVGNFSEGLASARSGAVRGGKYNTLSGGKYGFIDKSGNEIIAF
                     KYDGAGDFSEGFAYVKVGKKYHFIDKKEEELGLLDYDDVGDFSEGMAKVRIGNYFDGK
                     WGYIDTTGKRVIDVKFEEAGDFYKGEASVKLNGKSMYIDKTGSNDEERKKIAEQEAER
                     KKQERKALVDWAIANAIRDTLAKEVLTLYLNEDNKVFNKVTDLQKNVESTMIFSEKYG
                     FDKRNWIIMSTEIDNKAKVAREQINTYKSMLKKYEPIREKNELIGKISTNLFRMNEFV
                     TACELWSQYINTLGAHDNGKTELDRVADSLIDLKKSDDNLRSFIQLYKKRNSL"
     gene            46386..47300
                     /locus_tag="PKU82_00490"
     CDS             46386..47300
                     /locus_tag="PKU82_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOZ81381.1"
                     /translation="MSTVLISGGTGLIGRHLCKKLIGKGYSVSILSRTGFTEGEIPVF
                     HFDTDKNEIDERAIREADYIVHLAGANIGEKRWTKNRKQLIVDSRVKTAQLIFEKVKE
                     YNVKLKAFISASAIGYYGAITTNKKFSETDSPYTDFLGETCRLWEKSADSFEEIGIRT
                     VKVRTGVVLTSQGGALAKMITPVKLGIGSAIGSGKQFIPWIHIDDLCNIYIKAIEDTQ
                     MHGAYNAVAPDFKTNREFTKEIAKVLKKPFWFPDVPDFIIKLLFGEMSEMILKGSSVS
                     PDKIINAGYEFIFPGLEGALKNVLKIEE"
     gene            47350..48153
                     /locus_tag="PKU82_00495"
     CDS             47350..48153
                     /locus_tag="PKU82_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOZ81382.1"
                     /translation="MNRKYLLLTIFLFSCASQKNLNNSTVQHNSLTADGRLFTSVFQQ
                     QSAEYKALCFQAYNIAELRLDQAIGSTTSVKPKAIITDIDETVLDNSPYSVHRALEGK
                     DYESVSWVDWTARAKADTLAGALTFFKYAASKNVEVFYLSNRAEKERVGTLENLKKFG
                     FPNADNAHLILKTDISSKEGRRQKIAETYDVLLLIGDNLADFSSLFDKKTTTERTQNV
                     QSIADEFGKKFIVLPNANYGDWEGALYHYDYKRTPAQKDSIMKSVLKNY"
     gene            48319..48759
                     /locus_tag="PKU82_00500"
     CDS             48319..48759
                     /locus_tag="PKU82_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ81383.1"
                     /translation="MASINPHINFNGNAEEAFNFYKSVFGGEFAKIVRFKDLASPEMP
                     IDKKEENKIMHIALPIGKHNVLMANDVPEFMGRTNENENRSKIVISAETKEEADKLFN
                     GLSAGGTVEMPIADSPWGSYFGMFRDKYGIEWMVDFDSRYKGQI"
     gene            complement(48815..48888)
                     /locus_tag="PKU82_00505"
     tRNA            complement(48815..48888)
                     /locus_tag="PKU82_00505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48852..48854),aa:Met,seq:cat)
BASE COUNT        15301 a         8438 c         9721 g        15556 t
ORIGIN      
        1 taggaaaggt gtagaagaac tcagaaatga tgcagagttt gttcgtctta aaaacaatga
       61 gttttttgaa gaaattcctg ttgatgaagt tcttggcaaa aaagctgaaa gcaaagatgc
      121 cacaccacgc agagattttc tcaaattttt aggttttggt gttgcagccg cttcaatggc
      181 agcatgcgaa gctcctgtta gaaaaacgat tccttacctg ataagcccgg agaacttagt
      241 tcccggaatt ccaaattatt atgcatcaac attttttgat ggccatgatt atgcaagtgt
      301 cgttgtaaaa actcgtgaag gtcgccctat taaggttgat ggtaatgaat tgtcaccact
      361 tacaaaaggt ggtgctaatg caagagttca agcttcagtt ttagatttgt acgatagcga
      421 aagaattaaa agcccaattg ctaaaggaaa agcagcaagt tgggatacag taaataaaga
      481 aattaaagaa aaacttgcag ctgctaatac aaaacaggca aaaattcgca tagtttcttc
      541 aaccatcatt agcccaacaa caaagaaggc tattgaaagt tttaccacaa aatatcctac
      601 tgctaaattg gttacttatg atgccatgtc acaatatggt ataataaaag cacatcaatt
      661 agcatttggt aaagcagcac ttccaaatta tagctttgat aaagctgatg taatagtaag
      721 ttttggagct gactttttga ataactggat tgcaccaatt gaatatgcac gtcagtatgc
      781 acagacaaga aagttagatg atggaaagaa aaccatgtct aaacacattc agtttgaagc
      841 agcactttct ttaacgggta gtaatgctga taaccgatat aaattaaaag caagtcagac
      901 aggaaatgct ttattgtcat tatataatgc aatagcttca aaagcaggtg ctgcaacagt
      961 aagtgccggc aagaccgatg cagatgctgc tattaacagc acagcaaacg atttatggaa
     1021 tgcaaaaggt aagtcgttgg tagtatgtgg aactaataat gccaatgatc aattagttgt
     1081 tgctgccatt aacaacatac ttggtaatta tggagcaaca atcagtatag ccaaacatag
     1141 taatgttcgt cagggaaatg atgaagctat ggctgaattg cttcgcgaaa tgaatgcagg
     1201 tgaagtagga gtagtaattt tttacaatgc aaacccatta tataactatc ctgatactac
     1261 tgcatttact tctgcattaa acaaagcaga agtaaaaatt tcactatctg accgtaatga
     1321 cgagacagca ataaaatgcg attatatttg tcctgattct cattatcttg aatcatggaa
     1381 tgatgcagag cctgtgcgca atatgttcag tttggtgcag ccaacaattc aaaaactgtt
     1441 cgatacaaaa caatcacaag aactattctt gttatggagc gatgcacctg taacagacta
     1501 tcatgagttt ctgatgcaaa actggagaca aggttttctg gcaacagcaa caggaatggc
     1561 atcagtacct gcatgggaaa aagttttaca ggaaggtgtt tggaatggtg aagttacaga
     1621 gagcgagcct gcatttgttg gaaacgtaaa tgatgcagca agtgcaattg gagctagaaa
     1681 atcaggcggt cttgatattg tagtttatga aaaagccggt ataggtaatg gacgtcatgc
     1741 aaacaatcca tggctccaag aattacctga tccggtttct aaaatatgct gggataatta
     1801 ttttgccata gcaccatcca tggcaaaaga aaaaggtctg gtacaaggaa atattattga
     1861 agttaagtca ggaaatgtaa cacttaaagg tccggtttta ctccaacccg gtcaggcaaa
     1921 agatacaatt gcagtagctg ccggttatgg tagaacaaac agtggtaaag ccgggaataa
     1981 agtaggtatg aatgcatatc ctttactgac tttttcagaa ggaacattca actataccaa
     2041 tagtggagca agtttaaata aaacagttga ggctgattat acacttgcta caacacaatc
     2101 tcaccacacc atgatgggtc gtgctattgt taaggaaacc acacttgaag agtatattaa
     2161 agatccaaag gcgggtaatg aagaagagaa atttcattct tataaaggaa aagtttcagc
     2221 taaagaatta gacctctggg caacaaaaga tcatccgggc ttcgatcgcc cgattaatgc
     2281 atggggtatg tctattgact taaactcatg tattggttgt ggtgcctgcg tagtagcatg
     2341 tacagcagaa aataatgttc ctgtagtagg caaagaagaa atacaacgca gccgcgagat
     2401 gcactggatt cgtattgatc gctattatac cagcgatatg gataaagaaa aagcacatga
     2461 gcagggtgta ggtaagttaa gcatgtatgg ttttatggaa gagcctgcag agaatcctga
     2521 aattgtattt cagcctgtaa tgtgtcagca ttgtaatcat gcaccttgtg aaacggtgtg
     2581 tccggttgct gcaacaacac atagtgaaga cggattgaat atgatggcct ataaccgttg
     2641 tgtaggaaca cgttattgtg ccaacaactg cccctataaa gttagacgtt ttaactggtt
     2701 taaatattct gataatccac aatttgacta taacatgaat aatgattatg gcaaaatggt
     2761 gttgaatcct gatgtaattg tacgctcacg tggagtaatg gaaaaatgct caatgtgtgt
     2821 tcagcgtatt caatatggta agttagaagc aaagaaagca ggccatccgg taaaagatgg
     2881 agctataaat acagcatgtg cacaaacttg tccgaccaat gcaattactt ttggtgatta
     2941 taatgataag tcaagtcgtt tggtaaaaca aagcggtagc gaacgcatgt atcatttgct
     3001 tgaagaactt aatgttcaac cttcaattaa ttatttaact aaggtgcgaa acactacagg
     3061 caaaacaaat aacagtcata aagcataaat aaaattaatc gaactaatag aaatttttca
     3121 acaatatgtc gaatgtagaa tcaccgctta ggccgacact catcgaagga gataaaacgc
     3181 ttcatgatat taccgaagat gtttgcagac ctatagaatc taaacccaac aaatactggt
     3241 ggatggtatt tagtgttgcc tgtgctgcac tgttatggtg gattatttgt gtagcctata
     3301 cagttggaac aggacttggt gtttggggtc tgaataaaac agttggatgg gcttgggata
     3361 ttaccaactt cgtttggtgg gtaggtattg gtcacgcagg gactttgatt tctgcaatct
     3421 tattattgtt tcgtcaaaaa tggaggctat caattaaccg ttctgcagaa gcaatgacca
     3481 tatttgccgt tatctgtgct gcctcattta ttgtagcaca catgggtcgc ccttgggttg
     3541 catattggcc acttccattt gcaaaccaat tcggttcatt gtgggtaaac tttaattctc
     3601 cgttggtatg ggacgtattt gcaatctcga cttatttctc tgtatcatta gttttctggt
     3661 acacaggatt gattcctgac tttgctgcag ttcgtgatcg tgcaaaaact aaagctcgta
     3721 aattctggta ttcagcctta agtatgggat gggttggttc agctaaaaac tggcaacgtt
     3781 ttgaagaggt ttctttagtt cttgcaggta tttcaacacc actggttctt tctgttcact
     3841 ctgtggtatc aatggacttt gcaacagcat tggttccggg ttggcataca acaattttcc
     3901 caccatattt cgtagccgga gctatttttt cagggtttgc aatggtgcag acactgctga
     3961 taattacacg taaagtatta aacttagaag aatatattac aatttatcat attgacatga
     4021 tgaataggat tattcttatt acagggtcta tagtaggttg tgcttatctg acagaatttt
     4081 tcattgcctg gtattcaggt gtagaatttg aacaatatgc atttatcaat cgtgcaacag
     4141 gaccatattg gtgggcttat tggagcatga tgacttgtaa cgtgatttca cctcaattga
     4201 tgtggagtag taaactcaga agaaatcttt tcgtaacttt cttcttatct attattgtaa
     4261 acataggaat gtggttcgag cgttttgtga ttatcgtaac ttcattacac agagactttg
     4321 ttccttcaag ctggacaatg tttcatccta cttgggtaga gattggtctc tttattggtt
     4381 cgataggttt gttctttgtt cccttcttac tctttacacg atatttccct gttatagcaa
     4441 tgagcgaatt aaaaacaatt ttgaaatcat caggctctca atcaaagaaa ggagtacaac
     4501 atcatgacta aacaactgca tggcattttt aatgatgatg aagttctttt aaatgcaatt
     4561 aaagaactaa agtcaaaagg tattaaatgt actaatgcct attcgccttt tcctgtacac
     4621 ggacttgata gtgccttagg tcttaaacga acaagaattt ccatagcctc ttttttatat
     4681 ggtgctacag gaacatgctt ggcactttta atgatgtggt acatgaatat ttgggattgg
     4741 ccaatggata taggtggaaa gccaagttgg aacttatata agaatatccc tgcatttatt
     4801 cccataactt ttgagtttac agttttatgt gcagcacatg gcatggtgat tactttttat
     4861 ctgcgaagca aattattacc tggagttact cctaatgtta ttcatgatag aatgcatgac
     4921 gatcattttg tattaaccgt tgaaggaaaa gtaagtgatg aaacaactat tcgcgaatca
     4981 ttgaaacaag ttggtgcagt ggaaataaga tcataaagaa ttaaaacgga tgaaaaacac
     5041 acacatatat tttaatgtaa ttattggagg catgatattg cttagtgctt gtggtgataa
     5101 acaatcaccg ggcttggagt atatgcctga tatgtatcgt actccagcgg tggtagctta
     5161 ttctccatca cacatcacaa aagatagtgt ttctgcattg gcacccgttg ccggaactgt
     5221 tgcaagaggt cagtatgttg actttattta tcctaacacc aatgaaggat atgaggctgc
     5281 aggcttagag ttgaagaacc cttttcaacc aacagcagaa aatatggagc gtggtaaaac
     5341 agaatttgat acctattgtg cccactgtca tggggagaag ggtgatggta aaggatggct
     5401 aaaaattaaa ggagatccat ttcctgtgcc atcttataca agcgcagcga ttaaagactt
     5461 acctgatgga aaaatgtttt tctctattac ttacggtaaa aaccttatgg gatcacatgc
     5521 agcacaagtt agtgctgacg atcgctggaa aattatatta tacattaacc atttacaggg
     5581 agacgaacca ggcacagttg tagctgacag ttcggctact gcacaaaaca aataacataa
     5641 cgcagcctat ttaagataat actatgcagg acaatctcac atataccttt tcaagtaagc
     5701 tgaaaaccct ttcgtttatt ctcatggcga taggtgcaat ttcaatcgga ataagctttg
     5761 ctaccaatcc ggaccaaaca tgggcaaacc tcatgcacaa caacttttac ttcttaacca
     5821 tagcattagg aggtctgttt ttttatgcag tacaatatgc agcagaagta ggttggagtg
     5881 tttacatcat tagaattcca ttagcgatgt ctcaatatct gtggattagc ggtgttatta
     5941 tgctcatttt gttttttgct gccggacatc atatttggca ttggacacat catgagttag
     6001 ttatggaaac attgcctgat ggaaagccta atccggacta tgatgaaatt ttagcaggaa
     6061 aaagtgcata ccttaacgtt ccattctttt taattagaat ggtagcatac tttgtaatat
     6121 ggtatggctt tgcagttttg ttacgtaagc atgcaatgaa agaagattta tcaggtgcaa
     6181 tagaacatca taaccgcagt cgcaaactca gtattatatt tcttgtactt tttgctgtta
     6241 catcatctac ctctgcctgg gattttgtaa tgtcaataga ctcacattgg tttagtacat
     6301 tatatggctg gtacactttt tcaggaattt ttgtaagctc attaggtgta atcgctttga
     6361 ttattgttta tctacgcagt cagggatatc tacatcattt aaacgaaaat cacttacatg
     6421 atgtaggtaa gtttattttt gccttcagca tattttggac ctatttatgg tttgctcaat
     6481 tcatgctcat ttggtatgct aaccttccag aagaagtaac ctattttatg gaaagacaag
     6541 attattataa aacacttttc gtaactaatt tcttagtcaa cttcattctt ccatttgtag
     6601 ttttaatgac tcgtgatgca aaaagaaaat tgcgtttatt ggcaatatta ggctctgtaa
     6661 tttttattgg ccattggata gatacatatc tgatggtagt tcccggaata atgcatgaaa
     6721 aatggacaat tggagtattg gaaattggaa caacccttgg gtttatgggc atgtttatct
     6781 ttaccgtatt tactgcactt accaaattat cacttcttcc tcaaaaacat ccaatgcttc
     6841 aggagagcga acattttaca ttgtaatttt taaaatagac attaacacaa atcagtaatt
     6901 tcaagacatg acactattaa tcacaatatc aatcttactt ggcattcttg ttttagtcag
     6961 actgatgact attgtacaat tatcctcaga actaactggt agcagcgatt ctcaagaaga
     7021 acagattaga cacaataatt caaatggatt tggatttatt gtatttatgg tcttaggtct
     7081 aggtttattt atctatatga ccatagacta ttccaaacac ctgcttcctg tatctgcttc
     7141 tgaacatggt gttggtttag ataaactttt aaatattaat tgggcaatca ttggtgtagt
     7201 attctttatt acacagatag ccttatttta tttttcatac aagtatcgtg acaataaaaa
     7261 gcatgaagct tacttttatc ctgaaaacta taaactggaa ttaatctgga caattgttcc
     7321 aaccattgtt ttaactacat tgatcattac cggtctgaaa caatggaatg gtatcttttc
     7381 tgaaaaagaa aacagtatga atgttcaggt ttatggttat cagtttgctt ggataacccg
     7441 ctatagcgga cctgataata cacttggaaa atcaaactac aaactcatta ctgatgataa
     7501 tcctttaggt ttagatgcaa gtgatcctgc cgtaaaagac gatatctata caacaggaaa
     7561 tgaaatgcac atacctcttg gacaagagtt gaaatttcag tttaatgcac gtgatgtaat
     7621 tcacagtgca tattttccac acttcagagc acaaatgaac ttggtgccgg ggatgaatac
     7681 atttttctcg tttaaaccaa caattactac agcagaaatg cgcaagatta ccggtaatga
     7741 taagtttgac tatgtattgc tttgtaacaa aatatgtggt gttgcacatt acacaatgaa
     7801 aatgaaagta gtagttgaca cgcctgcaga ttttaaagca tggttgaaat cacagaaaaa
     7861 agcttctgaa gtgaaagcag cacctccggt aagtgcatta aacagtactg taaaaggcag
     7921 taactcaatt taaatttgat attttctttt gttcgatata atttaggcta agaataaaat
     7981 tacaatggaa gtaaaacaaa cagcaggtgt tgcagtagag catcacgatg atcatacaca
     8041 tgagcacgat catcacgaaa catttctgac aaagtatatt ttcagtcagg atcataaaac
     8101 cattgcaaag caatatttaa ttacggcaat tatcatggca ttcttcggaa tgtatatgtc
     8161 attaatattt cgtttgcaat taggatttcc ggaaaccaaa tttgcttttc ttgaaagttt
     8221 gattggaaaa tggggtaaag gtggaagatt ggatcccggc ttttatatgg cacttgttac
     8281 gatacatgga accatcatgg tattttttgt attgacaggt ggattgagcg gaactttcag
     8341 taacctgctt attccattac aagttggtgc acgtgatatg gcatcgggct ttttaaatat
     8401 gctgagcttt tggtttttct tcctttcttg tgcagtaatg acatattccg tttatattga
     8461 aacaggccca gcagcaggag gatggacagt atatccacca ttgagtgcct taccgcaagc
     8521 aatgcctggc tctggacttg gaatgacgct atggttggtg agtatggttt tctttataat
     8581 tcagtctttg cttggtggta ttaattatat ctgtactgtt atcaatcttc gaacaaaagg
     8641 aatgagtatg acaaggcttc cattaacagt atgggcattt tttattacag caattcttgg
     8701 tgtattgaca ttcccagttc tttttgcagc ggctttactt ttggtcttcg acagaagttt
     8761 tggcacaagt ttttatcttt cagatatttt tattggaggt gtagcattac ctcaatcagg
     8821 tggaagtcct gtattgtatg aacacttgtt ctggttctta ggtcaccccg aagtatatat
     8881 tatcattctt cctgcatttg gtttgacatc tgaagtattg tcaacaatgg gacgtaaacc
     8941 aatatttggt tataaagcaa tggttggttc attaatggca attgcatttt tatcatgtat
     9001 tgtttgggga caccacatgt ttgtaacagg gatgaatcct ttccttggtg cagtgtttac
     9061 atttacaact ttactagttg caattccttc ggctgtaaaa gtgtttaatt atttggctac
     9121 actatggaga gcaaaaatac aactcacacc agccatgtgt tttgctattg gattagtgtc
     9181 attatttatt tctggtggat tgacaggaat tatccttggt gattctgcat tagacatcaa
     9241 tgttcatgac acctactttg ttgttgccca cttccacgtt gtaatgggtg cctctgcaat
     9301 attcggaatg ttagccggag tttatcattg gttcccaaga atgtttggac gtatgatgaa
     9361 taagacgtta ggttatgtgc atttctggat aactctaatt gctgtttatg gggtgttttt
     9421 cccaatgcac tttattggtt tgggtggtgt accaagaaga tattattcca attctgctta
     9481 cgaaggcttt gatgtgtttt taaatataaa caccttaata acggtatttg cttttcttgc
     9541 agcagttgct caagtaattt tccttttcaa ctttgtttat tctatattta aaggaaagaa
     9601 agcaccacaa aatccatggc atagtaatac acttgaatgg acatcacctg caggcccagg
     9661 tattcatgga aactggaatg gtcctgtacc cacagtacac cgttggcctt atgactatag
     9721 caaaccgggc gcagatgatg attatattcc acaaacagtt ccgtattctc aaacaaaaaa
     9781 ctctaacctt ccgggtgaag agtaatgtga attaatagtt ttttcagatg agctactttg
     9841 caaactgcaa agtggctcat ctgttttaaa ttactatttc atagttgtta ccaagagtaa
     9901 atattaggtt attaaacata tttaaattga taattttgca ccaatgaaat cttcttttag
     9961 attgattaga caatgcattt ttgttttttt gattgcttta atattcaata gcgataatct
    10021 gtttgcattt tctgactcta cgaaagttgt tgtcaaaaag cctaaaaaag agaagtacta
    10081 taaacggtca agaattgctg ttgaagatgt taggaattat atggatagta taggaatttt
    10141 aaattctgat atagtcttaa aacaagcatt gctcgaaaca ggacacttca aagccaaact
    10201 tctcatggac aaaaacaatt tgtttgcatt tcgttttacc aaacgctata tgagttttat
    10261 caattggcaa gcaagtgttg actattataa aaaatggcag gataaatttt ataccaacca
    10321 gtcagaaaat tactatgatt ttctgaaaag aataaggtat gcgcgttctc cacattacat
    10381 taaagtttta aaacaggtta aggcgcgtac tgctcaataa agccaatcat aagctgtgaa
    10441 aaattcccga aataacgagc ctattaaaat tctgtttttt cttacagtta ttttatctgt
    10501 aatctcgata atgccaaaat atttgactgg gagcaagaaa attaatctct atagcgatat
    10561 tattcataag gaaaataaaa aggaaaaacc gcaaagtttg gcaattgtaa acgattctgt
    10621 attttttaaa aatgatactg ttaagcaggt attaacagac acagcaaaat ctactgcgac
    10681 taagataaat tataagtcag gtgaacttga tttaacaagg ttctttgaag cattgcacaa
    10741 tcatgaaaat gaaaatggaa aaatcaggat tgcatggttt ggtgattcta tgatagaagg
    10801 tgatttggtg acacaggact tcagaaaact gatgcagaaa atttatggtg gcagcggtgt
    10861 tggctttgtt cctgttactt cacctgtagc acaattcagg caaacaataa gacaaacttt
    10921 ttctgatgac tgggaagttt atagttttat gaaaaaacca aaacagaatt ataaactggg
    10981 aataggggga tacagttttg tttcatcaaa gggtagttgg gttgattata aagcaagcag
    11041 attctacccg gtatattcag aggtaaaaat gatttgtcgc cagtgtccac ctaaaagttt
    11101 tattttgaac gaagacactt ctttagtttt ccctgttgat agtattggta gttttaaaga
    11161 ggttgaatta ataaggaaca atcctataaa ggaagttaaa ttaaccttta aaaaaccaaa
    11221 ttgtgaagta catggacttt atttcgataa aggaaacggt gtttatgtag ataatttttc
    11281 gtttcgtgga aattccggaa ttcctctttc caatattcct tttggtgaat taaagtctct
    11341 tggcgcaaaa gaaaattaca agttgataat tctaagctat ggtttaaatg tagttgctca
    11401 tgatgtgaaa aactatagtt ggtatgcttc atcatttcag aaaacattaa actacattaa
    11461 agaagcattt cctgatgcat caatcttagt catgagtgta ggtgataaaa gttatcgtgc
    11521 agaagatggc tacgaaacag agccagatat tcccattttt gttgaattgc aaaaacgaat
    11581 tgcacaaaga agtgatgtag ccttttataa tttatatgaa gcaatgggag gttataacag
    11641 tatggtaagt tgggcagagg gtaatccgca attagcaaat ttagactata cacatcccaa
    11701 ttttgcaggt ggaagaaaaa ttgcaggaat gttgtatgat gatattatta aaagctatga
    11761 tagatatagt aaaacggtgg aaactatgga agtgaaaaga gatacaatga ttaagcctca
    11821 ggcaaaacaa tgaaaagaat aattttaatt ttttctctgt taatatattg tacaaaatgt
    11881 catgcacaga gtgattccga tactacaatg cctgtgttgc aatatgagaa atttgttgaa
    11941 tataaaatgt ctttattgaa atttcttaaa cccgatgcaa ttgatattga acagaaagaa
    12001 gcattgaaaa atttttatag taaattgttg cagagtagag ctgacgggaa aaaagttagt
    12061 atagtgcaca ttggcgattc gcatttacag gccggatatt tttcaggtat ggttcgcaga
    12121 ggactacaac aacgctatgg taatgccggt aggggaattg tatttccata caaacttgcg
    12181 cgttctaacg gcccgcatga ctatgtttct ttttctcaga atagctggaa aggaaataga
    12241 aatgtaatta cacaagattc tttatcaacc ggcatcaccg gattttcagt aacaaccaac
    12301 gacagtagcc catctgtagg aattagctta cgaaataatg aaggtataga ttatagattt
    12361 aacagagttc aagtatttta taaacctgac agtactttta tgactgcaag tgatagttta
    12421 gcaataattg aaacagtaaa cccaataaaa cttgcaacag gaattaatga gttcagattt
    12481 tctgattatt tatcgaattg taaaattaac atcagttctg atactactta tacattattc
    12541 ggtttaaatc ttttgaatga tagctctggt gttgtttatc ataatattgg tgtaaatggt
    12601 gcagagtata aaagttttct tgggcaacca atgttttttg aacaagtaaa agaactgaat
    12661 gctgacctgt atattctttc atttggcaca aacgaggctt tcgcaaagta ttttgatgga
    12721 acagtattta ttcagaccgt tgattccttt ataactaaac tcaggaatgt ttcaccaaca
    12781 gcgtcaatat tgattacttc accaacggaa tcatgtaaac gtataagacg tggtgtgtat
    12841 gtacctaatc caaatgtaat tgctattaaa aatctccttc agaaatattg catggataat
    12901 aaaatagctt tttttgattt atatgagtgc tcaggcggca agggaagtat gcttcgtttt
    12961 gcaaaaaata aaatgagtga tgccaggcga attcacctga accgttcagg atatgaaatg
    13021 cagggattaa tgttgcttca ggcgcttctt aaaccattat ctgaacaatg acaatagatt
    13081 gggaaaagct tggacatcag tttttgtatg ataaagcaaa tccattgctt ttcaacagtt
    13141 cgttttttct tttttatttg ttgttgtttt tgttcgtatt tagacttctg caacgaacag
    13201 gaactttacg aattgttgca gtaacacttt tctcacttta tttcttttat aaagcctcag
    13261 gagtttatgt gcttttggtt cttgcggctg ccgtaattga ttttaacctc agtaatttta
    13321 tttacaatgc aacatctgat tttcggagaa aagcattact cgttttcagc gttgtcatca
    13381 atcttggact acttgcttat ttcaaataca ccaacctgtt aattgacttg tataatgcca
    13441 tactaaccgg aaatgttcag ccaatagatg tctatctgcc aataggtata tcattttaca
    13501 ctttcgaaaa tctgagttat actattgatg tttacagaag acagtttgtg ccggtaaaaa
    13561 aatttattga ctacctgttt tttctttcat ttttcccaaa actaatgatg ggaccgatag
    13621 tgcgtgcagc agatttcata cctcagataa gaaaagagat tgcactcact gcacatgatg
    13681 tatctaaagg cttgttttta attctgaccg gattgtttaa gaaggttgtt atttcagact
    13741 ttatctatct caatttagtt cagtatgtgt ttgatgatcc tttgcggcac acaggtatgg
    13801 aatgtctttt tgcagtgtat ggctatgcat tggtaattta ttgtgatttt tccggctata
    13861 gtgatatggc aataggtatt gcaaagtgga taggtataga tatcaatatt aactttcttg
    13921 caccctatca gtcggcctcc ataacagagt tctggcgcag atggcacatc tcactttcat
    13981 cgtggttgcg cgattatctt tatattccgt taggtggtaa tcgtaaagga gaagtaagaa
    14041 cctacattaa tttatttatc accatgttgc ttggtggact atggcatggt gccagcttga
    14101 attttatttt ttggggtgca ttacatggtg tggcattagc ttttcataag ttatggaagg
    14161 cagcatatcc atcacatttt tccggacgtt taacacgcat tgcaggcatc attataacat
    14221 ttcattttgt ttgtctttgc tggatatttt ttaagtgcac aacctttact gacagtttta
    14281 acatgattca tcagattgtg tttaacttct atgctgaagg ttggacatta ttcagtgcca
    14341 attatagtga agtgttgtta gtgatgttac taggatttgt attgcacttt ttgccaatcc
    14401 gatggaatga aattattatc aattatactg aaaagtggca ttggttacta aaggcatctg
    14461 tagcaacggc agttgtaatt tttgtaatcg ttcttaaaca agccaatcct gtcatgccgg
    14521 tttatcttca attttgataa aaaattctcg atataaatct atatattgaa atctttttta
    14581 attttaaatt tggtttttca aagtggagga aaaatgaaaa gactttttat tgtaatcagt
    14641 gtatttttta ttgcggcatg taaagacagt ggaaagcagt caataaaaga cacatctgta
    14701 ttgttaaagg aaattactga tggctattat caggagcgaa tgcgctattt ccctcttgag
    14761 gccacagcaa atgccgataa ccgttataat gatttactac caattgatat cagtgatgct
    14821 tatattgaca cacttcgtca attctatcac cgttactccg aaaagctgct tactattaac
    14881 aagcctgaat tgacaggtca ggatttaata agttatgagg tgttacaata tactttaaac
    14941 atagaggaag aaggcctgaa atttccttca aacctaatgc ctgtaaatca gttttggggt
    15001 atgcatctca cattttctca attgggttca ggcacaagct ctcagccatt taagactgta
    15061 aaagactatg ataatttcct gcaacgtatt tctgcctttg tagcttatca ggatacttcc
    15121 ataaataata tgaaaagagg aatgttacaa gggtttgttc ctgcacgtat cctcattgaa
    15181 agaacaattc cacagtataa atcactcatt gcaaacaaag ttgaagagag tgttttttac
    15241 gggccaatta aaaatatgcc cgatagtttt tctgatgcag ataaaaaacg attaacggat
    15301 gcttactcga atgcaatcct taatcaatta aatccaagct ttgaaaagat ggcagttttt
    15361 ttagagaaag aatatttgcc tgcttgtctt cctgttgccg gtatttctaa tattccggga
    15421 ggaaaagaat attataactg gcttgcgaat aaatggacaa caacaacaat gagtcctgat
    15481 gagatttata atttaggcct taaagaagtc gaacgtttac attcagagat ggataaagta
    15541 aaagcagaga caggttttaa aggatcactt aaagaatttt ttcatttcat tcatactgac
    15601 ccacaatttt ttccatttaa gaaagattct gatgtaattg cagcctatct tgcaatagaa
    15661 gacagaatga aaccacagtt gaaaaaactt tttaatcatg tgcctaaggc agcttttgag
    15721 gttaggcaga cagaagctta tcgtgcagca tctgccagtg cagagtataa cacagcagca
    15781 cctgatggat caagacccgg ggttttttat gtacctattt tagatgcaac aaaatataat
    15841 gttgtaggta tggaagattt gtttctgcac gaagccattc caggtcatca ttatcagatt
    15901 tcattacagc aggaaaatac atcactacct gagttcaggc gctttggttg gtttggagcc
    15961 tatggcgaag ggtgggcttt atatacagaa tcattaggga aggaattagg attatataca
    16021 gatccctatc agtattttgg ttgcctgagc gaggaaatgc acagagccat tcgtttggtt
    16081 gtagatgtgg gtattcacct gaaaggatgg acgcgcgaaa aggcaattca atattcattg
    16141 gataatgaaa tggaaagcga ggctgatatt actgcagaaa ttgaacgcta catggccata
    16201 ccgggtcagg cattgtcgta taaaataggt cagttaaaaa ttattgaatt acgtcaaaaa
    16261 gctgaagagc agttaaaaga taaattcagt attcagaaat ttcatgatca gctactttcc
    16321 tatggttgtc ttccattaag tgtactcgaa aaatccatta aactatggat taaagaacag
    16381 aaaaagcaag gctgaaatta cctttcacaa gtataagttt tcgtacaaat agcttatgtt
    16441 tgcagcataa cattttaagt ttttctgctg tatgaatatg aagcaactaa tcgagaattt
    16501 tccttcgcaa atgcgcgagg ctatgactat tggcgaaaca tttaaagcta caagctggcc
    16561 acatgaaata aaaaatgtat tggtgaccgg acttggtgga tctggaattg gaggaaccat
    16621 tgtttcacaa ttgtgtgaga aagagatgcc gatacctttt atggtgaaca aagattattt
    16681 tatgcctgca tttgtaaaca aacattcgct ggtagttgta agttcctatt caggaaatac
    16741 agaagagaca cttcatgcca tggatatggc aataaaacaa ggtgcaaaag ttgtatgtgt
    16801 cagctcagga ggcaaaacag ttgaattagc aaaacaacat ggattggatc atatattaat
    16861 tccgggtggc atgccaccac gttcctgcta tggatattca tcaacacaac ttttctatat
    16921 actacatgct tttaaactta taggtgatgg ttttaaagcc tcattgaaga atgctgttga
    16981 tttgttagat gcagaatctg ctaatattaa aaatgcatca cataagcttg catcagagct
    17041 gattgggaaa attccagtca tttattcagc ttccaataat gaaggtgttg ttgtgcgttt
    17101 cagacaacaa gtgcaggaaa atggaaaaat gttgtgctgg catcatgttt ttccggagat
    17161 gacacacaat gagcttgtag gatggacaca accacataat gatattgctg tagtgttgtt
    17221 cagagataaa gatgattttt tcagaacagc aaagcgaatg gatatttgta aagagatatt
    17281 tggtcgctac acaccacaca tttacgaagt atggagtaaa ggtggttcac caatagaaaa
    17341 ggcaatttat ttgattcact ttggtgactg gacaagctgg tatcttagcg aaatcagaaa
    17401 tatagactgt acagaagtga aagttattga ctaccttaaa agtgaaatgg ccaaactgaa
    17461 ttaattattc tgtttgaatc tgtcattcat gaaaaaagaa aatttattta tagcagtaga
    17521 gggaatagat ggtagtggta aaagtacaca ggtacaattg ctgaaagcca accttgaaga
    17581 agccggttat aaagtttatg caacaaatga acccacgaat tttgaagcag gaaaactcat
    17641 tcgttcaatt tttaatcata gagtaaatgc cgatcagcat accattgcag cattgtttgt
    17701 tgcagacaga ttgcagcatt tgcttgataa ggaaaatgga attctggcaa tgcttgaaaa
    17761 aggctatacg gtaatcacag atcgctatta tttttcgtcc tatgcctatc atagtgccta
    17821 tgtagaaatg gattgggtaa tgtctatcaa tgctaaatgc gcagcgttgt taaaacctga
    17881 cctgaatatt tttgtagatg tagatcctga aactgcattt aaacgcatta ttgctacacg
    17941 cacctcaaca gaattgtacg aaaccaaaga aaaccttcgt aaagtctatg ataattatct
    18001 taaagcattc aaattactaa agaatacaga aagcattgtt tttgttaatg gcaaaaatga
    18061 tgttcagcaa gtagcagctg atattttcaa aaccgtaaaa aatttattgc atacctaaaa
    18121 aaattgattg tttttttagt ttagtttttt atcattgctg aaattttaaa tcgtttcaaa
    18181 atgaataaga agcatccaaa ggcattgccc tacctgttcc tgtctgaaat gtgggagcga
    18241 ttcggtttct atctcatgct tggtattttt ttcctttaca tgacagacac acaacgtggt
    18301 ggcatggcaa tggacagaaa agaagcttca gatattttcg gaacattcat tgcattagtt
    18361 tacttgactc cgtttgttgg tggattaatt gccgatagag ttttaggata tcgtgtttca
    18421 attacacttg gtggcattct tatgggtata ggatattgtg gattggcaat acccggcatg
    18481 actgcatttt atgtttcact attactcatc atcattggta atggattttt taaaccaaat
    18541 atttcgacca ttcttggtaa tgtgtataac gatccggagt ataagccact caaagattcg
    18601 ggatacaata ttttttacat ggggataaac atcggagcat ttatctgtaa ttttttcggc
    18661 tcttatttgc gaaacaactt cagttggggt gctgcctttg ccgctgcagg gattggaatg
    18721 tttgtaggtg tcattatttt ttggattggc aataagcact atgcacatgc cgatgttcgc
    18781 aagcctgtaa agcccgaaga tatgcctgta tcaaaaatat taggtataac attattacct
    18841 gcattaattg cagggtatat tggctggata attcctgaaa atattttcgg tagcgattca
    18901 accgatgcat ttattttagg tgccctacct gttgccggat attatgtaaa tatacttttg
    18961 cgttcagata aagaggacag acggccacta gctgcattgc tggcagtatt tgctgttgca
    19021 atagtttttt gggctgtgtt taaacaaaat ggaacagcac taactacatg ggctgaaagt
    19081 tataccaatc gtgaagtacc acagagtgta gaacctgttg ctaaagtgct tggagtaaca
    19141 caaacagtaa ccttctcaaa agactctgta ccattaaccg atagtcagtt tcgtgtggca
    19201 cgtgacgaaa ataaaaaagc aataaaaaca tgggattacc cactttatta taagaatgtt
    19261 cctgcagata aattacctaa agaaggtgaa tcgctaacat taatttcaac agaattaggt
    19321 caatcaatta atccattttg ggttgtaata ttaacacctg tgattgttgc tttctgggca
    19381 tttctcaaaa gaagaaacag cgagccttct acaacatgga aaatatttta tggctttctc
    19441 attacatcat tgtcaacatt ggtaatggtc gcagctactt atgcatgtca taatggttta
    19501 gagaaaagtt ctatgtggtg gctcattgca agctatggag taataactgt tggtgagctt
    19561 tgtttaagcc caatggcatt gtcattggta tcaaaactaa gtccaccacg acttacagca
    19621 ctgatgatgg gcggatggtt tttatccaca tcaataggaa ataaattgag tggcgtgctg
    19681 gcaagtatgt gggatgaata tgaacataaa gcaaactttt tcttagttaa tttcacatta
    19741 gtaatgataa ctacaataat tattttactg atgttaaaat ggcttaataa aattgtgaaa
    19801 gagcatggag cgtaatagta tattattgtt tttattctga aagtttattt gcattttatt
    19861 cttgaattag tacggcagat aaaaatgttt aaactctttt tagtagagag gttttttaat
    19921 ttgaaaattt caacccctca catgataata tcagaccttc acccaaaaca tatattactt
    19981 gcacagattt tgaagtatta taggtataat tcaaaatccg agtattatga gattactatc
    20041 tactgctgtt attttattag gatgcctctg tgcacaagct caaattagta tcacaaataa
    20101 tgaaatgcct tctgccggtg atacagcacg ctttacaaaa gctgttgtaa atccattgat
    20161 aaactttagt gcaacaggca ccaatcacac ttggagtttt ggtaacctcc gcaacaatgg
    20221 acaagattta aaatcttatc aaaatgtttc atctacagga tttatttatt ctttgttttt
    20281 ttctaatctg ccatttaatc caaacagagc aaacgtaaca gaacctggac aagcattacc
    20341 ttcaaaccct ttgttgacaa ttaccgatcc atataatttc tattatcgtt caagcagtgt
    20401 atataaacaa gtaggcttcg gtgcagagat tgcgaatatt cctatgcctg ttgccttcag
    20461 tcagcatgat attatttaca acctgccatt aaattataac aattcagata cctctcattc
    20521 agcatggaat atcggtcttc ccggcttagg ttattatgga cttacacaga caagaattaa
    20581 tcacgttgat ggttggggaa cgcttacaac accacatgga acattcaaca ccttacgtgt
    20641 aaaaacacaa ttgtatataa aagatactat aagtgttgac acattaaatc tgaattttgc
    20701 tttaaacaga ccaaagacaa cacaatataa atggttagcc aataatgaaa ttattcctgt
    20761 gctacagatt acaaccacag aaattttagg gttagaagta gtgacagaaa ttatatatcg
    20821 tgatgattac aatacggtat caccaggaag tttaaatgca gcatactgtg ccggcagtaa
    20881 tgtatcaatt ccatatacag ctacaggtag tttcaatggt tccggatttt tacaaactgc
    20941 taatagtttt acagcacagt taagtgatgc cactggcagc tttgcaaatg ctgtaaatat
    21001 tggaagtgtt acttcaagag tttctgggaa tattaatgca gtaattcctg caaatactcc
    21061 tgtaggaaat ggttacagaa tcagaattat atcttcaagt cctgcattaa caggaggaga
    21121 taatgggttt gatttaagaa ttgagaacaa tgcagttgct tcaattgtta atcagggttt
    21181 atcggcattc tgcagtggca actctgcttt attacagtca gttaatgttg attccggata
    21241 tacttatcag tggcaactaa atggcacaga catttccgga gctactacag ataactattt
    21301 ggcatcagca acaggcaact atacattaaa cattaccaac tcatgtggta ctattacatc
    21361 taatgctgct gcaattaatg ttttgccaat gccaacggtt caggttactg catcaggacc
    21421 attaacattg tgtctaggta ccacagttac actttctact ttatttgatt ctacatattc
    21481 ctatcagtgg caattgaatg gtgttgatat ttccggtgca acaacagaaa attacactgc
    21541 tgcaagtgga ggagattaca cagttgtagt gagtaatagt tgtggtaatg taacatcgtc
    21601 tgttcaaaca gtttcagttc aatcagctcc agttgcagga agtctcactg cagcttcgtc
    21661 aacaatatgt catggtgatt ctgtacaaat taactattct ttgcagtctg caggcattac
    21721 ctggttgcat aatggaaata ttctttcagg cattactacc aacagtcatt ttgcaaattc
    21781 tgccggtgtg tattctgtcg ttgaaagtga cttatgtggt agtgatactt ccgttgctgt
    21841 aatactgaca gcggacagtt taccaactgc tgtaataact gcttccgggg cattgtcatt
    21901 ctgtgcagga ggagatgttg tgcttacagc agcaaccggt aataattacg catggcaatg
    21961 gtatatgaat ggaacgcctt tggcaactac gcagttaata acagcaacac agagtggaaa
    22021 ttatgaagtg gaaattacaa atgcttgtgg ctctgtcatg tcaactcttg ttgcggtgac
    22081 tgcattccca ttacctgtag taccgacaat tacactttct aacgattcat tattagcgac
    22141 aacgactgta ggtacttatc aatggtttta caacggcaat gccattaccg gtgctacaaa
    22201 tgacttttat gttccgtcac aaaatggaaa ctacagctta atggttacag atgctaacgg
    22261 ttgttcatcc atgtcagcag tgtataatat gacaacagtt ggattgcaaa ctatatttaa
    22321 acaagcacat aatgtgtttc caaatccgtt taacgactat ctgacaattg aattaccaca
    22381 gacagaaaat gcaattattt tgactgttga aggaaagcaa ttgtggagcg gtacgttgca
    22441 tcagggtaag aatgtattga atatgcagca atttgttagc ggcatgtata ttctgcaact
    22501 gaacaatgaa aatggaatca caaactacaa attagttaag cgataaacat tgtgttatct
    22561 gaacaaaagg caatttgctt tttgttcaga tttaactgtt atgatttgcg gtatttaact
    22621 cctctgaccg catttcaaca gtagtctgtt ctgtttcagg agttgtttca atgtgcttaa
    22681 aaattgttcg gttaaaaaga ataattaata aaacacctgc agtaattgct gaatcggcaa
    22741 tattaaaaac aggtctgaaa aaaataaatt cttctccgcc ccaaagtgga aaccattgcg
    22801 gataatgtgt ctgaataatc gggaaataaa acatatccac tactttacca tgtagccatc
    22861 cggcatagcc tccggcatcg ggcataaagg ttgcaacacc aaattcgctg gaggaaaaaa
    22921 ggcggccgta aaaaatactg tcaataatat taccaactgc accggcaaaa attaatgcca
    22981 tagctgtggt gtaaatgtga tgcgcttttg ttttatgcag atgccataca taccaggcta
    23041 atgcaaacat gaccccgata cggaaaagtg aaagaaattt ttttccgaac aattccatgc
    23101 caaaggccat tccattattt tcagtaaaat gtatgataaa ccaattgcct gcaatatggt
    23161 attcctgacc gagatacata ttggttttaa tccagaattt caaacactga tctaaaatca
    23221 gaattacgga aacaagagca atatattttt tcaattgtat ggattgatat aaaatgagga
    23281 ttgccaaaaa tgttgacaat cctcgtattg tgtcataaat aaatattaat cgcgatactg
    23341 ttgtaccttg gcttcaatgc tttgtgtagt gtgtggcaca gcacgaagtc tttcttttgg
    23401 aatcaacttg ccggttacac gacaaaggcc atacgttttg ttttcgatac gtaccaatgc
    23461 attttcaagt tgctcaataa acttacgttg acgcgcagca agttgtccgg cttcttcttt
    23521 tgctaaagtg tcgctgccat cttcaagcat cttaaatgat gaagcagtgt cgtcagtacc
    23581 atgttcattg ctgttgttaa gaaatgcctg caggttgttt aactcaactt ttgcatcagt
    23641 caatttttga ataatcaact cgcgaaactc gtttaattct gcatcactgt agcgtgtttt
    23701 atccgggctg ttttccaact ttgtaggtct gttgtcaagc attgcaaccg gttttttaag
    23761 aatagaagca acaggtgcat ctggaagaac agggtcttca cgtaatggcg tagtacgcgg
    23821 aggagcagcc ggtttaacaa ttggagggtt gctgcgtctg cgtgaacttt ctcttggctt
    23881 ctctacgtgt ttaatcatag atgttgccga tttgctttta tcctcttttt gtgattttgc
    23941 agttacacca ggctttataa ttttaattgg ttttggctta acaaccattg caggtttaac
    24001 aacctttgct ttagcaacag gttttgcctt tgctactttt tttgcaactg ctttgctttt
    24061 aattactttg ctttttgcaa cagccttctt agttacagtc tttgatttag caggtttacc
    24121 ttttgctact gcttttttag caactacttt ttttgctaca ggcttacttt taacagcagc
    24181 cttttttacc actttaacaa cttttttaac agctgttttt ttattttttg gtgtagcttt
    24241 ttttgctgca gccttttttg gtgcagcttt ctttgctaca ggctttttct tcgctttact
    24301 tgccattttt aaatcctttc tatttcaatt aatgttttaa ctccttcttc aacctctatt
    24361 tcatcggcta ccggtgtagc taatgtttct acccatgtaa atgaggaggc taaaatttcc
    24421 gcgcaaatat agtctaaatt actttcgatt gcagtgatta tagatggctg atttttaagt
    24481 ttaaccagta ttttatccgt tacctcaaat cccttgtctt tacgcaggtt ttgcactctg
    24541 ttaatcagtt cacgggcaat gccttcgtca cgaagttggg gcgatatact gatgtctaat
    24601 gcaaccgtta aacccccatt actattgacc tgccagccgg gaatatcttc tgagctgatt
    24661 tctacatctt cgaccgatat tgcaactata ttttggttga ttctgaggtc aaatgtgcct
    24721 ttactttcta agttacgtat gtcatcctga gtcatttgtg ctatggcagc agcagcatct
    24781 ttcatgagtg cccccagctt cttgcccagc actttaaagt tggctttaat tttctttacc
    24841 aacaatcctg aggcatcggt aacaaactcc atttctttca catttacctc agtaagtatc
    24901 agtttttcca cctgttcaat agccatttgt tgctttgggc tgtccacagg aatgagtatt
    24961 ttttgcaagg gctgtcgcac tctgatgttt actttcttac gtaatgccag taccatactt
    25021 gaaatacgtt gtgcaaaatc cattctttcc tctaaggcag tatcaatcat tttagtttct
    25081 gccttggtca gataggtaag gtgtacagag gtatattgca atggtgtttt agctgcaatg
    25141 gcattgctac gcatgctatc ggtaagattt ctgtaaagcc agtcggagaa gaaaggcgct
    25201 accgggctca tcaactgtgc tgtaaccatt aaacattcaa acagcgtttc ataagcagat
    25261 tgtttgtcgg tattcatttc gctgcgccaa aaacgtcttc gtgaaagacg tacataccag
    25321 ttgctgagat gctcatcaac aaattcttcg atctgacggg ctgcaaatgt gggctcataa
    25381 tcgttcagac tatccgtgac acgctgcact aaattgttga gctttgataa tatccagcga
    25441 tccaattcag atctgtcctg cacgggtact ttattttttt catcaggaac aaaaccatca
    25501 atattggcat atagtgcaaa aaacgaataa gtattgtata gtgttccgaa aaattttcgt
    25561 tgcacttcgg caatgccatc tacgttaaat ttaagattgt cccagggctg tgcattgctt
    25621 atcatgtacc aacgtgtagc atcggggcca tacttgccaa tagtctcaaa cggatcaaca
    25681 gcattgccta agcgtttact cattttattt ccgtttttat caagcaccaa tccgtttgac
    25741 acaacatttt taaaagctac tgaatcaaac agcattactg caatggcatg taaggtaaaa
    25801 aaccaaccac gagtttggtc aacgccctct gcaataaagt ctgccggata gcctgtgtta
    25861 aacccttcgt tgaaaggcat tggtaggcct ttacttaatt tttcctgatc taattgccat
    25921 tgtgcatagg gcatagcacc actgtcaaac cacacatcaa tcaaatcagt ttcacgatac
    25981 atttttttac ctgtatctga aatcagaata atgttgtcca cataaggtct gtgaagatca
    26041 aagtcagatt tagaagcatc tacatgttcc agatgatgtg catccataaa acctgctgct
    26101 acagcttttt caatttctgt acgaagctca ataatagagc ctatacattt ttcttctttt
    26161 tcatcttcac ttcgccatat aggaagagga attccccaat aacgtgagcg cgacagattc
    26221 cagtcaacta agttttcaag ccagttgcca aaacgaccta tacctgttga ctccggtttc
    26281 cagttgattg ttttgttgag ggcaatcatt ttgtctttgc aggctgttgt acggataaac
    26341 caactgtcta aaggatagta taaaacaggc ttgtctgtcc gccagcaatg cggataatta
    26401 tgctcgtact tttctacttt aaatgctttg ttttcctgct ttaatttgat ggcaatgcgt
    26461 tcatcaactg acaaatattt atctcttcct tgtttctgac gctcggcttc tttttcattc
    26521 gtagtgagat attcttcttt tacatactct cctgcaaagt cagtaacttc tttcacaaaa
    26581 cggcctctct tatcaactaa tggtgaagga tttccacctt cgtcagtaac taaaattgca
    26641 ctgatgcctt gttgttttgc agtacgaaaa tcgtctgcac caaaggttgg cgctatgtga
    26701 acaatgccag ttccatcttc tgtgctaaca aaatctcctg caacagcaac aaaagctttt
    26761 ccgtcaggtt ttacataggg taacaattgc tcataagcaa ttcctgtcag tgtggaacct
    26821 ttacattctg caattacttt aaaaggaatt tgtttgtcac cgggtttaaa atcttcaaga
    26881 ctaagtgctg catttttttc actaaaatat tttgcgaaca aatctttggc aagcacaaca
    26941 acaataggtt cgttggtgta ttgattaaac gtcttcaccc acacataacc gattttttca
    27001 cctactgcca atgcgctgtt tgaaggtaat gtccagggtg ttgtagtcca tgcaagaaaa
    27061 taaaagtcac aatttatatt ctgcaaatct ttttgtaaag actgtggcag tgtttctttt
    27121 tttattctga actgagccac cacagtggta tctttcacta ttttataagt gcccggttgg
    27181 ttcaattcat gcgagctgag acctgttcct gctgccggag aataaggctg aatggtgtag
    27241 cctttataca acaaatcttt tttataaagc tctttcagca agtaccagca agtttcaatg
    27301 tagccatttt caaatgtgat gtaagggttt ttcagatcta cccaatagcc cattttacgt
    27361 gtcaggtcat cccacacttc tttatacttc atcacctctt tgcggcaggc tttgttgtaa
    27421 tcttcaacag atattttttt acctatatct tctttggtaa tacctaaagt tttttctaca
    27481 ctaagttcaa taggcagtcc atgagtatcc catccacctt tgcgctttac ctgatagccc
    27541 tgcaacgttt taaaacggca aaaaatatct ttaatggcac gtgccataac gtggtgaatg
    27601 ccgggcaagc cgttggcact tggcggccct tcataaaaat tataagcctt agcgccctgg
    27661 cgtgaggtga tgctggcatc aaaagtttgc tcgttatccc atttcaatag aacgtcattg
    27721 gcaattgctg ccaaatccaa ctgcttgaat tcaggatatg gtttatgact cattacactg
    27781 ttttttcaaa gagtgcaaag gtaagaagtt ctgctatagt ggaggatgag ggaaagttga
    27841 cattttttgg aaagaaaatg acctgaaatg ccttttttaa agcgatttta aaatatctgt
    27901 ttttattttc atgatgacat ttgttattcg taactttgaa gattaatagt aaaaatcatt
    27961 acaatgaaat cattattcct ttcagtactt ttttatgtac tatcaaattt tactatggcg
    28021 caggtagcct ctaaattagc ttcaaaaccc gatgcagcta aagagttgat tacagttgaa
    28081 acttcttgcg gagaatgtaa ttttaaactg cccggagata attgcgattt agcagtgcgc
    28141 attaatggca ctgcatatta tgttgatggt gtttcaattt ctgaattcgg tcatccgcat
    28201 gatgacaatg gattttgtgt tgctgtacgc aaagccgaag ttcagggaga agtggtaaac
    28261 aaccgtttta aagcaactta ttttaaacta ttgccggttg ccaatgcatc agaaaattcc
    28321 aataaacaag atggaggtaa gtagcttttt agttcttatt atctaccact ctgtttaaac
    28381 ccctgattaa tgctatcaat ataatccaac acctcctgac ggccactttt tgtatcggca
    28441 gaagtgacaa aatatggagg tagtgttgcc caggttttat ataactgttc gcaaaactgc
    28501 tttacagaaa aatcgttttt ggcacgtgtt tgtttatctg ctttggtaaa aaccaaacta
    28561 aatggaatag acttttcacc aagccagttt ataaagtcaa gatctttttg ttgtggcgaa
    28621 attctgatgt caattaacac aaaaacagac atcaggtttt tacgataaat catgtaattg
    28681 cgaatcatac catcccattt ttcgcgctct gtacgagcag tttttgcaaa tccatagccg
    28741 ggtaagtcaa ccaaaaacca ttgcccattg atgataaaat gattaatcag ttgtgtttta
    28801 ccgggagtag aagatgtttt tgccagacct ttcttattac aaagcatgtt gatgagtgat
    28861 gatttgccca cattacttct gccaataaat gcatactccg gtttattggg ttccgggcac
    28921 ttttttacat ctgtattgct ttgaacaaat tctgctgaac ttatttccat aattttaatc
    28981 aatgcaaaag taaattttaa tcttcaaccg gaatcatgta gtttcgtatc cgctttttac
    29041 aatgagaaat tctttttata tcttgatttt cgctcttgtc gttctgacct catgtcataa
    29101 cgataaaagt tccgataatc caccatgccc tgcaatggaa atgcacatca gtggcgatac
    29161 tacagtagtt gccggtgaca gcattggctt aatggcaact ttgtttaccg gggccaccta
    29221 ttcatggaca ggcccaaatg gttttattgc caacacctat aatcctgtaa tttccgaatc
    29281 atcatcatta agcgaggggt attactatgc aagtgcagac attaatggac tttgcaagag
    29341 taatgttgac tctttttatg ttactgtaat ttgcgatcgt ccggaagatg tgcttgccaa
    29401 ttacattaca actattcaaa ccaacgacac cattcatctg tttgcacact ctattaccga
    29461 ttctgttgaa tatatatgga cagggccggc aggatttact tctacacagc ccaatccaat
    29521 catcaccaat gccattccgg gcaatgaagg tgtttatact gtttatgcgc acaaagatca
    29581 ttgctatagc aagcccgaca gtgttgtggt aagttttaat cagtgcaacc catcgtccaa
    29641 cacatttgca aacacattag atagtaatta ttttctgtat tcctatggca gtgctaatct
    29701 ttaccctaac acaagaagta ttacaggaac ggggcttaac ggaacttcgc aattgcgtgc
    29761 tacgttttat ggcaacaatc caccaacagg aaactatatt ataaatccgg cacattgtcc
    29821 tgcaagtgga agtggagcac ttgcacccgg tgaagtttgt gtggaattca gtaatgcatc
    29881 aggaacttat caggcagtaa gcggtacggt aaaattaaaa gcattaaaca cattcgattt
    29941 ctgcgatgca ccattccgat tacagtttac aactactaca ctttttaacg gcagcggcaa
    30001 ggttcagttt taatgtgccg gatgataggt gtttacatgt ctggcttttt tgctttcgta
    30061 aatatcatca atagaaatta aaataccggt ttcttccaca tcgccaaatt catgatttaa
    30121 agcagtgcca aaagttttca ttgttgctga aagattcatg tagctgttaa acagtggagg
    30181 aatattttct ccaagttcgc gcactttttt attcaaatta tggtgacctt ctttgtaagg
    30241 aagatttttc aggcttgcta caaaatcatg tacatcattt tctgccggta atgggtggtg
    30301 tggataaacc attttatctt tatcaggaaa atagtgttgc ataaaagcaa gaatcaaatc
    30361 tctggcaatg cggttaaaac gcaaatacat ggttaccttt ccaaaaaaat atttcatgtc
    30421 tggattatca atggcaatag cacctaaccc atcccataaa ttgtcgagag aaaaaaggcc
    30481 tttacgattc tctgccgaag gttgatattg tggttgtaca aacgagcggc ctaattcaat
    30541 ggtatgcgga aaatagtctt tgataaattt ttcggtgaaa tgaaacagtt ctgttgtggc
    30601 taacttgtat tgactgtgcg ggtcagtggc agcatcggca cagcgtatga aacgatagcc
    30661 accaacaatt tctttatcgg caggattcca aactataagt tgcttgtagc aattttcaat
    30721 aagatcaaac tcatctaaat caagtgcttt tcctgtgcca ccaccggcag ctctgaatgt
    30781 aagctcacgc acacggccaa tttcttgcag tacattaggc gagtcatgag ctgtaaccac
    30841 atacaacttg ttctgcccat tgttggcatc gcggataaaa cggccattgt ttaactcctg
    30901 ctccagcagg tgtctttcga ccgggtcaat gatttgcata cttcacgaaa atagaaaaag
    30961 tattttgatt gtatgaatgc agcaaaaaag atttgaaaca gcttttttgt atatctctga
    31021 aaaggataat gtcattaaac caaatttgat agctttgcac gctatttgaa tctttatcgt
    31081 aataaaatga aaaacggact tttaatttac tgtgtactgc ttactctggc agtagcttat
    31141 ttattttaca aagtaaacag ctgtacaagc aattgtgctc ctgcaggcgt tgccactgcc
    31201 atgaatgatg gcaatattgt ttttgtaaat gttgatacat tactcgagca ctacgactat
    31261 tatattagtc ttcgtgagtc gcttaaaaag aaacaagaca gcattgatgt agttctgaaa
    31321 aacaaaggca aagaattgga aggcgaaata gtaaattacc agaaacgtgg tgccagcatg
    31381 acagatcagc agcgcggtgc cgaagaagaa agattaggac acaaacaaca acagttggtt
    31441 gaatataaaa accatttact tgatatgttg actgaagaag aagctgtcat gcaggatagt
    31501 ttgcacaaac atttagttgg ttatcttaaa gacatgaata agcaaactaa ttttaagttt
    31561 gttttcggct atcagcgcgg caatggaatt ttattagcca acgattcgct cgatattaca
    31621 caaagtgtta tagaaggact gaacaaaaaa taatcatctt ttaaaacttc gcaccaaaag
    31681 tcatcataaa acgattgatt ttggtattaa ttgatgctga tggcgcactg ccatctgcta
    31741 aggtgtaagg tgcataattc ccgcttgttt tagtataggc ataacctaaa tcaagaaaaa
    31801 agtttccacc tttaattcct aagccaccgg tgtagctcac tgcactacgg ttaccttctt
    31861 tgtaagttat tccaccttca taggggcttg attgcattac agttccggcc ctgaaactga
    31921 aaattttata tcggtattca gcacctattt ttaaaatctg accatttttg agacttgctt
    31981 ttatatctct gttttcgtca gaaaaactgt actcattgga gttgagtcgt gctgtagaat
    32041 aatctaagta ttcatagtcg gcactaatca gtccatgatc aagaaatacc aagcctacac
    32101 ttgtaataac tttaaaaggt gtggtcaggt tgtaagaaaa tctgccatcg ggtgatttgt
    32161 tttccaaata tccttcagaa aaatttgcat aaatttttga agaatactga tcgttcatct
    32221 cataataggt tggtgtgtga atggccaatc ctaaccgaac catatctgta ggtttaaaaa
    32281 tgacacctaa tttcaggttt acgccattgc cttgcgttga aagcttttgt gtgtattttg
    32341 aatttttgaa agttttaccg ctcagactat ctttagttgt gttttgcaag gcatactcct
    32401 catatacttc cgttgaagaa tatctgaaat aaggaatacc gacagtagca ccaagataaa
    32461 cctgattttc gtagttagca gcaaaagaaa tattaatttc gccttgcgaa cccgtgcgtg
    32521 tgatgagacc tttttgtttt aaatcgccat tatagagcgc accctgatac tgatctatca
    32581 aactgctgtc tgcaacagga tttgtcagat aactgttata tgccaaacct gcatcatact
    32641 gatacatatc ttcaccggat aaatttgctt tgctgatgcc attactgatt tcagcaaaat
    32701 aatcattaat agaattattt ttatttattc cttcaaaagc aaatctgtcg ctgaatgagt
    32761 tgatacggtt gtagccgaat cctaaagatg ttcccatcca gcctgaagtt tttttctcgc
    32821 gtggaaaggc cagcacaaag cccagattgc ctacacctaa atttacttta ttgtctgatg
    32881 ttagtgtacc taaataggtg gcatcgctgt ttccaaattg caaaacagga ctgaaagtaa
    32941 actcgcttct gcgataaacg gcaataccgg ccggatttat tgaaaggcta gaaaagtcgg
    33001 cacctaaagc accaaaggct ccacccatac tcaaagcgcg ggcagtggtg ccaaaagttt
    33061 gttgcatact gtagcgcaat gcatcatttt tgttttgtgc aaaagctgtt gcggttaaaa
    33121 aaaagcatgc cgcaagcatg cttttaaatc tataatccat aatgtcgtta attgttattt
    33181 actgataatt atcttccgtt gcgtgaattg tttctggaag gagttgactc ttgtcgtgga
    33241 cgtgattcct gacgttgttg tgatggctca gagaagttgc gttcctgacg tggtgccggt
    33301 gttgctcttt cctgacgagg tgtgtgttcc tgacgtggag gattgtttct tacaggagct
    33361 tcctgacgtg gagcagtttc actgtttctg ttattgcgtg gcgcactttc ataatttctc
    33421 cttggagatt ctgccggtgt attgtagcgt ggattgctct ctgtgtttct tcttggtgac
    33481 tctgcaggag tattattgtt gttacgagga ttactttcgt aattacgtgg aggaacagtt
    33541 tctgtgttgc gtggactgtt gtactcagga ttaacttcat ttctgctgtt gttgcgcacc
    33601 ggaggtgcag caggagtagt tgagttgtct tttacctcat tattgttgcg tggcgtagag
    33661 aactgatcgg ctctgctgtt ttttacaggt gctgtttcaa cattacgaga gtctttcacc
    33721 ggactattgg tgttcacatt gtttcttgga tgtattactt cagcatcatt attccttgca
    33781 ggtgcagttt caacattgcg agagtctttt accggactat tggtgtttac attatttctt
    33841 gggtgaatta cttctgcatc attattcctt acaggagctg tttcaacatt acgagagtct
    33901 tttacaggac tattagtgtt cacattattt cttggatgaa ttacttctgc atcctttgta
    33961 tcacgtgttg gtaatgacat tgcattggca aaactctgac ttacaggcat tgagtttctt
    34021 ccgtttttca ccaaagccct attgtacgaa tcggctatgc ttcttggcgg aacagcattg
    34081 ctgctgcctg aaccggaagt tgatcgtctt ggtccgtaat atgtgttgtt gtagctatag
    34141 ctgtcgtagc tgttaaaata gtatggattg tagccataac cattatagta accatcataa
    34201 taaccattcc agtacccgtt gttataacca ttccagtatc cataactgta attgttccag
    34261 ccatgatgat gatatgggcc atatccatag tatgaatatg gactactcca ccagtagttg
    34321 tagggtgtat aaccccaact gaaataagtg aatgaaggag cccaccatga tgaaggagcc
    34381 caccagttgt aaccgcaata aatgctcaca ccatagtcat aaggattata atcataccag
    34441 tatgagttgg tgtaatagga atcatagtaa ccatagttcc acattgggcg ataaaatctg
    34501 cgtatgcgtg atgaataggc atagtcataa taatcatcat cgctgtaata attattagtc
    34561 acataggtgt tgccatcctg tgttgttgtg cctgcatcgt tgtaatcaaa acggttttct
    34621 gcagaggtgc tgctgccctg atttgataca gattgatcgc ttacatcctg ctgaggttgc
    34681 tgcacttgtt gctgtgtctg cacggtttca gcaggtttgt ttgctttgga tgaataataa
    34741 acgtcatcgg aataggcatt tgaagcctgt tgtgatgaag tgcaggaagc aagtgtaaat
    34801 attgctgctg cagatagtgc tattgatttc atcgtatttt gatttttaat gtttgacatg
    34861 tttttataca caatagttta acgtaattgg atatgttatg tttgttttac aattttgctg
    34921 ccatctcata aaattcaaca gttttgcaaa aatcattcat tgcaatttca cttcaaagat
    34981 aaaaaatggc taaagacttt ccaacacgcg aacaaaatta ttcagagtgg tacaaccaac
    35041 ttgtcattcg tgcaggcctt gcagaaaatt catctgtcag gggttgcatg gtcataaaac
    35101 cctatggtta tgccatttgg gagcgcatgc aacaacagtt agacagcatg tttaaagcaa
    35161 caggacacag caacgcttat ttccctctct tcatcccaaa atcattcttt agcaaggaag
    35221 caaaccatgt agaaggtttt gcaaaagaat gtgccgttgt gacacattac cgtttgaaac
    35281 aatccgatga tggaaaaagt gtcgtagttg accccgatgc aaaacttgaa gaagagttga
    35341 ttgtacgtcc tacatcagaa acaattatct ggaatacata cagaaactgg attcagtcct
    35401 atcgcgattt acctatattg ctcaatcagt gggctaatgt ggtgcgatgg gaaatgcgta
    35461 cacgtttatt tttgcgcaca gcagaatttt tatggcagga aggacatact gcacatgcca
    35521 cagcagaaga agctgttgaa gaaacaagaa aaatgcttga tgtttatgct gaatttgctg
    35581 aacaatggtt agctattcct gttgttaaag gtgtgaaatc tgctaatgaa cgttttgccg
    35641 gtgcattaga tacctactgc atagaagcac tcatgcagga tggaaaggct ttgcaggcag
    35701 ggacatcaca ttttctcgga cagaattttg caaaagcatt tgacgtaaag tttaccacca
    35761 aacaaggtgt tcaggatttt gtgtgggcaa catcatgggg tgtctcgaca cgactgatgg
    35821 gagcattggt gatggctcat tctgatgatg atggtttggt gcttccacct aagctggcac
    35881 caattcaggt tgtcattgtg ccaatttata aaaatgatga gcaactaaaa cttgttactg
    35941 acattgcact taaaattaaa aaagcactca ccgcacgtgg cattgccgta gtttatgatg
    36001 acgatgaaca agccaagccg ggatggaagt ttgcagaata tgaaatgaaa ggggtgcctg
    36061 tacgattggc aattggaccc cgtgatgtag aaagcggcac agtagaactt gccagacgcg
    36121 atacgaaaga aaaaatgact gttcagataa cagatattga attgaagatt gaaaatcttc
    36181 ttgaacaaat ccaacagaat atttttaaga aagcagttga ctttagagaa acatcaacgc
    36241 atgtcgctga tagtatggac gagtttatta aaatactcga cactaaggga ggatttgtat
    36301 ttgcacattg ggatggtaca gctgagacag aagaaaaaat taaagaacaa accaaggcta
    36361 ccatccgatg tattccatta aacaacaaac aagaaaacgg caaatgtatt cttacaggta
    36421 aaccgagtac acaaagagtg ctttttgcac gagcttatta aaattttaaa actatggaac
    36481 cggtaaacga taagatagca gaactcatta aaacacattt tccactcaac tgcaagatag
    36541 ttgagtttgc atcaggtcat tttaatgaga taaaaaacta tctgaaaaaa tttgttgatg
    36601 gtacggcatc ttattatcaa ccacccaaca agcgtcagcg tataaaattt tatgatacag
    36661 gcagcaatca gtgtgaattg caactgggtg atgacacgct atggtttcat ttacatccaa
    36721 atttatttcg tataagtcct gataatcctg cttatcgtta ttcctatctg cgcgatgatg
    36781 aatatcgcag ccgctgcgct gttattcacg tttataattt tctgtcagac tctcttgccg
    36841 agcgcaggag tgatgactat ggtatgctcg ttgccagaat ttttatcaac tacgaaaatc
    36901 attttatggt tgaaggacgc aaacagttag gcatgttgtt taacgacatc agcaatgata
    36961 ttttgactga acaacggttg aatgatgtac tcgatcaggt aattttgttt tgcttacaat
    37021 atgatttgtt tgcacaacct tttgatcagg ttccggcaat gagctatcgc gaaatttctt
    37081 ctggttcgtt tgggtctgct gttgcaagta gtaaacaact tggcttcaag ctgaaggctg
    37141 aagatcatga tacggagtaa actttaaatg cagttactta aaaaaacctt aattacaatt
    37201 gcaagtattg atggtgtctt taaattttta tcaatcgcca atttttagat ttcttttcag
    37261 attttagtgt aatgatgtaa gagccatcag gcaaagttga aacatcaaca gcatactgat
    37321 attttatgtc ttttgtcatt ctgctgattt gttttccaga gatgtcggta ataataattt
    37381 cagcattgtt tattatttca ttcgtaacaa gattgaaaac actcacagtt ggattaggga
    37441 atgttatcca atttgaaact gaaaattcat tgatgcctac tgtagtaacc gagtgacatg
    37501 atgaggtgtc agtacatccg tttgctgaga tgataactgc atagctgcca ttggctgtgg
    37561 ctgtgaaagt ttgattggtt gcacccggta ttggactaaa gttgttgctg caatctaccc
    37621 attgataagt tgctccgacc tgcacggctg ttaatactgc ctgattctga ttcacagtag
    37681 tgtcattcga aaaaagtgta agtctcacta cagagtcgca gtttgcagat gaaaccaaag
    37741 tagctgtgta agtaccggcc tgtgtcagtg tttggccgtt aaaagaataa gtttgacctg
    37801 agcaaattgt atctgtaatg atggttgcat tgtcaatgcc atttacaaaa tttgatgatg
    37861 gaccattcat gctaaaattt tgtaaaacac cattgatatg gtttgaaata tcaggaatgg
    37921 taagaatacc ggaattgttt ccacctgctg tgccggtgtt catatcataa taaagttgta
    37981 aaccggcaga agtagcatct accttatttt tataaataca taaaatttcc tgaggtgtta
    38041 gcgctctgtt ccaaagagcc acttcatcca tttgaccatc aagaagaaaa ttagtgccac
    38101 taaaaacaag attacccata taaaatgatg tagcagtgct ggaaattaca ccggatgctg
    38161 aaacggaatc ggtaattaaa ccatctctaa aaagtcttaa atatgagcca tcataagtta
    38221 atgcaaattg ctgccatgtg ttgagttgaa gtccttgcga aagaatatca aaatttattc
    38281 ctgcgctgtt tctaaaacga gcttcaacgg ctgtggaaga atactgcaaa acatagaaat
    38341 cggctaccgt attatctctg aaccctgcca gtccgtcaaa gtcaggaaat ccaggaacag
    38401 gattgcgtgg atacacccaa aagctaagtg aaatttgcga tgaattagca atgagtgacg
    38461 aggcatttac agaaacaacc tgatcgtcta agccgtcaaa atcaatcgca ttttgactct
    38521 gagctgatac ggaatttgta aatgaatata tcagcaatac aattacaaaa gcatatattt
    38581 ttttcatctc ctttattgtt tattaatttt tacaaatata aaaacattca gtacttattt
    38641 tcttgattat gttgtagtta aaattcgctg ttacctacgg atgtattaaa ggcatgtaaa
    38701 ttttaagtaa gaatttttgt ggcaagtcga aatcgcattg attgtataaa ctaatgaaaa
    38761 gtaaaaagta attaaataaa gctgatttat tgaagttctt ttctgcaaag ccgaatcaaa
    38821 acattacttt tgcatcccgt atcaccacac ccctacttat gagcgctgta aagtatgttt
    38881 ttgtaaccgg tggtgtttct tcatcattag gcaaaggaat tatttctgct tcacttgcca
    38941 aattgttgca ggcacgcggt tatagagtta ccattcagaa attagatccc tacatcaatg
    39001 ttgacccggg cacattaaac ccctatgaac atggagaatg ttatgttacc aatgatggtg
    39061 cagaaacaga cctcgacctt gggcattacg aacgtttttt aaacgtcccc acatcacagg
    39121 caaataacgt taccaccggc cgtatttatc aaaccgtaat taataaagag cgtgaaggag
    39181 catttttagg taaaacagtg caggttattc cacatattac cgatgaaatt aaaagacgta
    39241 taaaaatatt aggcgaaacc ggtgagtatg atattgtaat tactgaaatt ggtggaactg
    39301 ttggtgatat tgagtcattg ccgtttgtgg aatctatccg tcagatgatt tgggaaatgg
    39361 gtagcaattg ttgcgttatt catcttacac ttttgccata cttaaatgtt acaggcgaat
    39421 taaagactaa gcccacacag cattctgtaa aaaccatgct tgagtatggt gtgcagcccg
    39481 atattttagt ttgccgcagc gaaaaacata ttactgccga cttgcgcaga aaaattgctt
    39541 tgttctgtaa cgtaaatgtc aatgcagtta ttgaagctgt tgacgctagc accatttacg
    39601 aagttccact gcacatgctc aaagagcagt tagacaaagt tgtgctcaat aaactaaaac
    39661 tttccttcaa gcaagaacct gacctcacag catggaaagc ttttttaggg aaattaaaac
    39721 atcctgtgtc tgaagtaaaa atcgggttga taggaaaata tattgagctg aaagatgctt
    39781 ataaatctat tgccgaagca tttattcatg caggcgttgc taatgagtgc aagttgaata
    39841 ttgagtggat acattccgaa aaaattgaag aagaaaatgt tgccgaaaag ctaaaaggct
    39901 tgtcaggaat tttggtggct cccggctttg gcgacagagg tattgaaggc aaaattgcag
    39961 ctataaaata tgctagagaa aataaaattc cttttctggg aatttgctta ggtatgcaat
    40021 gtgcagtagt agagtttgcc cgcaatgtac ttggactgaa agatgcacac agctctgaaa
    40081 tgaataaaga gacaaaaaat cctgttattg atttgatggc aaaacaaaaa gaaatttctt
    40141 caaaaggtgg caccatgcgt ttgggcgaat atccttgtgc acttgcaaag ggcagtaagg
    40201 tggctcaagc ctatggaaaa actaaaatct atgaacgcca tcgccatcgt tatgagttta
    40261 acagcaagta tcagaaaaga tttgaagatg caggcatgat tgccacagga ataaatcctg
    40321 ccgacaactt agtagagatt gttgaaatta aaaatcatcc ttggtttgtt ggagttcagt
    40381 ttcatcccga atataagagc acggtagaaa acccacatcc tctatttgta agatttgtaa
    40441 aggcagccag cgaatttgcc aaaggcgcca ttcagattta aggcagttgc tctaattcag
    40501 tttttaatgc cggtaaggta atagaattca ttactaacgg tatggataca tcagtgctga
    40561 cgatgacaga tgatttagaa aagaaataat tgtcgttgac cttcgccaat gccataaccg
    40621 ttacatgcag attgacagga aaattttcta cttcccacac gcctaaggta acagatttgg
    40681 ctttgcagat ggtgttgctg ccatccacag aaacaaaaac ggcagtattg gaagtttcgt
    40741 agtctgtacc tgtgtcaaca gtaatttttt ttctgctgct attactgaaa accggctctg
    40801 ctgcattttg ccaacctgta tttttcatca cataggcata gtgcgactga ctggaagaag
    40861 ataggagatt aatcatttgt gtatcggcat cagcagtcca tgaaacatca ctgctattat
    40921 tttcaagagg taatgagctg ctgtaataac gatgcagcat ttgcccgttg ccaaaaacag
    40981 cgttgagaaa aaagttgatg gttttgccat tagacaaact taatgcaaca tcattctgat
    41041 atgctgctag ataaaaaata ccacctgttg taagaaattt atttcccgat tgtgttgtca
    41101 gatttgaaag aataatatca cgcttgctga cacattcacg cacctttata tcaatgtttc
    41161 cacttacagc actgccatct gttttggtaa aacttccggc agaaatcata aaagtatttc
    41221 ctgctgctgt tgtttggtta atgttggccg atgacgaaaa ggaaaattgc tgcacaggag
    41281 cccctgtttc atcgaaaaat tgctcaatat cttttacagg agtatcgtat ttactgcctt
    41341 cctttttgca ggataatgta gcaatactaa taaatagcat aagcaataaa gcagaaaaac
    41401 gatgattcat tttttagaac tttaaatgca ataatagaaa ggtaaaatgt ataagtcaat
    41461 agacttgcaa cagagttata attgattttt acacatttta gaaaagcaga acatataaaa
    41521 attagagtag taagacattg agtgtcatca gttgtaagaa ataatactga ttttttacct
    41581 caacagagtc cctcctgatt tttaatcagt ggagtgaata gaccaaagat agaaatatca
    41641 taaagcgtag gaactgttat cttcgcagca tgtcaaaacc aacaatttta gttaccaacg
    41701 atgatggaat tactgcaagt ggcattcgtg cattggtaga agccatgcgc gaaataggtc
    41761 atgtaatagt agtagcaccc gacagtccgc aatcgggtat gggtcatgcc atcacaatcc
    41821 acaatccttt aagacttgat aaggtagatg tgtttaaggg agtagatgcc tggcagtgta
    41881 gcggcacacc ggtagattgt gtaaagcttg ccgtaaataa aatattgcat cgtaagcccg
    41941 atatctgtgt aagcggaatc aatcatggaa gcaattcatc tatcaatgta atttattcgg
    42001 gcaccatgtc tgctgcaatg gaaggtgcta ttgaaggcat tccaagtatt gggttttcat
    42061 tggtaaatta taaactcgat gccgatttgt ctgcagccaa acattatgca ggagtcatta
    42121 caaaaactgt tcttggcgga ggattgcatc ataatttatt actcaatgta aacattcctt
    42181 atctgcagct tgatgaaatg aaaggattaa aaatatgccg tcaggcaatg gccaagtgga
    42241 aggaggagtt tgatgaaaga ttagaccctg cacaacaaaa gtattactgg cttaccggaa
    42301 agtttatcaa tcccgatttg ggcgaagata ccgatgaatg ggctttggca aatggctttg
    42361 tttctgttgt tcctgttgag tttgatttta ctgctcatca tgccattccc gtattaaaca
    42421 aattgcagtg gaatgtttaa gaaaattccg gatacatttc atattggcgc actgtctgct
    42481 ttggccttgt cagtgcttgc gtggctgctt atcagccgca cagaatcata tattgtaaca
    42541 tctgcattaa aagattatct ggcatttcca cgactgtatt ttatagtgtt tgccttgcag
    42601 cttgtttttt taagaataat gatgattaac tttcaaaaac aacactttgc ccgtgggtgg
    42661 atgtttagtg tttttatttt tgctctgttt gtattttacc tgataaaaaa atcgtaatca
    42721 tgttcagtaa agataagttt ataacaggat tcattggtgg catcattgca ccggtagttg
    42781 gtttttatct ctattatatt gcactgttcc gccaaatgga tctttccgga ttttacaaca
    42841 ccttggtttc aaacaacacg cttgctgcag ctatcagtct gagtttgctt gctaacttag
    42901 cactgttctt tgtgttcgat aaatttggta agttgaatgg ctcgcgcggt gtcattgcag
    42961 caacattcat ttatggattt tacattgttt atcttaaatt tttctgaccc cgatgcgcta
    43021 ctacatcatt gccggtgaag cctcaggcga tttgcatgca tctaatctgg cattgcaact
    43081 gcgtaaatat gatacaggcg ctataatgcg cggatgggga ggcgataaga tgcaacaagc
    43141 aggagtggaa attgtaaaac actatcgtga gctggcattt atgggatttg tagaagtgat
    43201 aaaaaaccta cgtaccattt tgcgcaatct tgaaatatgt aaagaagata ttcttgcttt
    43261 taatcctgat gtagttattt tagttgacta tccgggtttc aacctgcgca ttgctgagtt
    43321 cctgaagaaa aaaaacatta aggtgtttta ttatatctca cctcagattt gggcatggaa
    43381 acaatcgcgt gtacacaaaa taaaacgtga tgtggacaag atgtttgtaa tattaccttt
    43441 cgaaaaagat ttttataaaa agtttgatta tgatgtagtg tttaccggac acccgctatt
    43501 agatgaaatt gaaaaaatca aacatcaacc ggctaaggca ttgtcgcagt cagatataga
    43561 taaaaaaatt attgcattgt tgccgggcag tcgcatgcag gaaataaaag aaatgcttcc
    43621 tgttatgttg aaagctgttt ctgttttcaa aaattttaat cctgtaattg cagctgcacc
    43681 caatattccc gattcgtttt atcagtcatt ggccggtaaa gatattccca ttgtacatgg
    43741 tcgcacctac gatattcttc agcaggctta tgctgcattg gtaacaagtg gcacagcaac
    43801 tttagaaacg gcattattta aagtacctga aattgtatgt tataaaggaa accgcttttc
    43861 atacctcata gcacggcagt tggttaaggt aaaatacatt tcgttggtaa atcttattct
    43921 tgaccgtatg ttggtaaagg aactcattca ggatgatctg aatgaaaaaa cactgacatc
    43981 agaaatgaaa ttgttgcttg aggatgaaga gtacagaggt aacatccttg caggttatga
    44041 tgaattggaa gctaagttgg gaatggcagg agcttctgaa aaaacagcac gaaaaatggt
    44101 tgatttgctg aagggcactg ttgatcaata aaatttattt acttttatgc taaattattt
    44161 ttgtgaaaac gacaaactat attttagtac tcatagcaac cttaatgctt tctgcatgtc
    44221 agaagcagga ggcagaaaca gataacgaac cgcaagttgc agccacatgt gacgatggca
    44281 tcatgaatca gggcgaaaca gatattgact gtggaggccc gtgtccgtta tgtcaggcaa
    44341 caatgaatgc tatggttgat gccgacactt ttaaggctgt atttaatacc atcacaagca
    44401 aatatgcaaa cggtgcattt tttatgtcag gatcttctaa tctggggtcg ctgacagtta
    44461 tttattcagg cccattgaat ataggtgctg taagcaatgc acaagccact tatgtaaaaa
    44521 gttcaacctc ggtaacgtat attgcaagca atgctaattt aactttcaat gccatcaatg
    44581 ttacagatag tacagcagaa ggaacgtttt catttaatgc catcaatttt aattcaccta
    44641 ccgacacaat taaaattacc aatggtttct ttaaaagaat cagtataaag aataattgaa
    44701 gagacatagt agttgaatat aatgacacct tcagaaatta ttatactgaa ctttgaggaa
    44761 ataagaagca taaaactgtg gaatggacta ccccctgaaa attatttgtc agaacagttg
    44821 ctcattacaa tattgtaagt gagtttgaag gtgtttataa tttgttgaaa gatttttgaa
    44881 tcatagttcc caacagtaaa aactacaatt atttttgaat atgtattaaa aatagagtat
    44941 tgtaaccacc aaataatatg aaaaagatag ttgttttttt agttgcattg atgttaacaa
    45001 acattataaa tgctcaggat ttaaaacctt ttaaaagtga taaagagctt tggggctata
    45061 aagatgcaac cggaaaggaa attattcctg caaggtataa ttttgcatgg agcttcactg
    45121 aagggattgc aagggttaga aataagaaaa tgaaatatgg atttattgat gtaacaggga
    45181 aggaagtgat tcctataaaa tacgaagatg caaaagattt ttatgaggga ttggcagctg
    45241 tcaaatcaga tgataaatgt ggatttattg acaagacggg taatgtaatt attcctttaa
    45301 gatatgatga agtaggtaat ttcagtgaag gcttagcaag tgctagaagt ggagcagtaa
    45361 gaggcggtaa gtataataca ttaagtggag gtaaatatgg atttattgat aaatcaggta
    45421 atgaaattat tgcatttaaa tatgatggcg caggtgattt tagtgagggc tttgcctatg
    45481 taaaggtggg gaaaaagtat cattttattg ataagaaaga ggaagaactt ggtctgcttg
    45541 actatgatga tgtaggtgat tttagtgaag gaatggcaaa ggtaaggata ggaaattatt
    45601 ttgatggtaa atggggatat attgacacaa cgggcaaaag agtaattgat gtaaagtttg
    45661 aagaagcagg tgatttttat aaaggtgaag caagtgtaaa attgaatggg aaatcaatgt
    45721 atattgataa gacaggaagt aatgatgaag agagaaaaaa gattgctgaa caagaagctg
    45781 aaagaaaaaa gcaggagaga aaggctttgg ttgattgggc aattgcaaat gctataagag
    45841 atactttggc aaaagaagtt ttaacacttt acttaaatga agacaataag gttttcaaca
    45901 aagtcacaga tttgcagaaa aatgtggaat caacaatgat ttttagtgaa aagtatggtt
    45961 ttgataagag gaattggatt attatgtcaa cggaaataga caataaggca aaggtagcaa
    46021 gagaacaaat taatacttat aaatccatgt tgaaaaagta tgaaccgatt agagaaaaga
    46081 acgagttgat aggtaaaatt tcgactaatc tatttagaat gaatgaattt gttacagctt
    46141 gtgaattgtg gagtcaatac ataaatactt taggtgctca tgataatggg aaaacagaat
    46201 tagatagagt agcagatagt ttaatagatt taaaaaagag tgatgataat ctaaggtcat
    46261 ttattcagtt atataaaaag agaaatagtc tatagcttaa aaaagttcag tagaaacctt
    46321 tttaaaacct ttcgatttat attttgttga ttttttaatt atttaggtaa tttaagaata
    46381 aaccaatgtc aacagtatta atcagtggtg gtacagggct gattggcaga catctttgta
    46441 aaaaattaat tggtaaaggc tatagcgttt ctattcttag caggacaggt tttacagaag
    46501 gtgaaattcc tgtttttcat tttgatactg ataaaaatga aatagatgaa cgagcaattc
    46561 gtgaagcaga ttatatagta catcttgcag gagcaaatat tggcgaaaag agatggacaa
    46621 aaaacagaaa acaattgatt gttgatagtc gtgttaaaac cgcgcaattg atttttgaaa
    46681 aggtaaaaga atataatgta aaactaaaag cattcatatc tgcatctgca attggttatt
    46741 atggtgctat tacaacaaat aaaaaatttt ctgaaactga ttctccttac accgattttc
    46801 ttggtgagac ctgtcgcctt tgggagaaat ctgcagatag ttttgaggaa attggaataa
    46861 gaacagtaaa agttcgtacc ggtgttgttc ttacaagtca gggtggtgca ttagctaaga
    46921 tgattactcc cgttaagttg ggtattggtt ctgcaattgg aagtggtaag caatttattc
    46981 cgtggataca tattgacgat ttatgtaata tttatattaa agcaatagaa gacacacaaa
    47041 tgcatggtgc ttacaatgca gttgcgcctg actttaaaac aaacagagag ttcacaaaag
    47101 aaattgcgaa agttcttaaa aaaccttttt ggtttcctga tgttccggat tttataatta
    47161 agttgttgtt tggcgaaatg tctgaaatga ttttgaaagg cagtagcgtg tcaccggata
    47221 aaattattaa tgcaggctat gaatttattt tccccggcct tgaaggtgcc ttgaaaaatg
    47281 tgttgaagat tgaagagtaa aaatatttga aaatctaaat tacacctatc tttacgcaaa
    47341 catattttta tgaatagaaa gtatttgctc ctcacaattt ttcttttttc atgtgcatca
    47401 caaaaaaact taaacaatag tacagttcaa cataatagtt taacagcaga tggacgattg
    47461 tttacttcag tatttcaaca acagtctgca gagtataaag cactttgttt tcaggcatac
    47521 aacattgctg agttaaggtt ggatcaggct atcggcagca ctacttctgt aaaacctaag
    47581 gcaataatta cagatattga cgaaacggtt ttagataatt caccttattc agttcaccgt
    47641 gcattagaag gaaaggatta tgaatcggtt tcgtgggttg actggacagc acgtgccaaa
    47701 gcagatacac ttgcaggtgc actgacattt tttaagtatg ctgcatcaaa aaatgttgaa
    47761 gtattttatc tttctaatcg tgcagagaaa gagcgtgtag gaacgttgga gaatctgaaa
    47821 aaattcggtt tccctaatgc tgataatgca catttgattt tgaaaacaga tatttcaagt
    47881 aaagagggaa gaagacaaaa aatcgctgaa acttatgatg tacttttatt aatcggtgac
    47941 aatcttgcgg atttcagttc tttgtttgat aagaaaacaa caacagaacg cacacaaaac
    48001 gttcaaagta tagcggatga gtttgggaaa aaatttattg tcctgcccaa tgccaattat
    48061 ggtgattggg agggcgcatt atatcattat gattacaaga gaacacctgc acagaaagat
    48121 tctattatga agagtgtttt aaagaattat tagaagaaac atttctcgtt aagctaacta
    48181 ggatattgaa taattgtttt ttgtagtaga tatatccata gttttttgat tgataaattt
    48241 tggagtgtaa tcaaagacct gaagagtaat tttatagaat aattactgat aatataaaca
    48301 cttaaaaaat caaaaaaaat ggcaagtatt aatccacaca tcaattttaa tggcaatgcc
    48361 gaagaagcat ttaactttta caaatcagtt tttggcggag aatttgcaaa aattgttcgt
    48421 ttcaaagatc ttgctagtcc ggagatgccg attgacaaaa aagaagaaaa taaaattatg
    48481 catattgcat tgcccattgg caaacacaat gtattaatgg caaatgatgt ccctgaattt
    48541 atggggcgaa ccaatgaaaa tgaaaacagg agtaaaattg ttatcagtgc agaaactaaa
    48601 gaagaagcag ataaattatt taacgggctt tctgccggtg gcactgtaga gatgcctatt
    48661 gcagatagtc catggggttc ttactttggt atgttcagag ataaatacgg tattgaatgg
    48721 atggtagatt ttgattcaag gtataaaggg cagatttgat attgcttatt aaaaaaataa
    48781 ggctgtcaca ctttattgca acagcctcta cttgtagcga aggggggagt cgaacccccg
    48841 acctcagggt tatgaatcct gcgctctaac catctgagct acctcgcctg attttgaggc
    48901 tgcaaaagta gtattccctt tcaattgtaa aaacaaatta tagaaatttc cttcattgca
    48961 attggttttg aagccaattg gcaataatag atgttgcgcc tgcattagat ttcact
//
LOCUS       DAOOOL010000003        45328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_54_length_45328_cov_152.875489, whole genome shotgun sequence.
ACCESSION   DAOOOL010000003 DAOOOL010000000
VERSION     DAOOOL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 45328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_45328_cov_152.875489"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKU82_00510"
     CDS             <1..330
                     /locus_tag="PKU82_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ81384.1"
                     /translation="AHATSTPAGDGIEAKVISHVLGNKVPVSSTKSMTGHECWMAGAS
                     EVVYSMLMMQNDFVAPNINFEEPDEDTKAINIITSSMEHRINCFLSNSFGFGGTNSAL
                     IIQRFKS"
     gene            406..2322
                     /locus_tag="PKU82_00515"
     CDS             406..2322
                     /locus_tag="PKU82_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOZ81385.1"
                     /translation="MSVGDKLHEILIKYWGHSRFRPLQQEIIESVVGGHDTLALMPTG
                     GGKSICFQVPGIYLDGICVVISPLISLMKDQVDNLRARGIKASAVVSGMSKTEIEIAF
                     DNCIFGKSKFLYLSPERLTSSLALTKLREIKINLIAVDEAHCISQWGYDFRPSYLRIA
                     ELREEFPEVPVIALTASATKSVRNDIIEKLHFKNHNFFQKSFERKNLSYIVEYNKDKL
                     TRTAEILKSVKGSAVVYVNSRGKTKDITTYLNSQGINADFYHAGLSTADRNKKQIDWM
                     QNKIQAIVATNAFGMGIDKADVRTVIHADLPDSPEAYYQEAGRAGRDEKNAFAVLIYN
                     SADKADLELRFQNAFPEIKLIRLIYHALGNYLKVALGSGENISFNFDVSDFASTYNFN
                     PLVTINALKILAEAGYIHLSDAVYIPSRLKFTVGQAELYNFQVQHPESDKVIKTILRS
                     YGGSFDNFIRINEAELAKRLNIAQDALIRHLFFLQKNELIEYLQQTDKPQLTYLMARV
                     KQETVVPDIKLLEFRKQRFNERVNAMLHYADSLTSCRSMMLLNYFGETTNTRCGTCDY
                     CRKRNKIELNDLTFEKQNAEIKDFIQQQTLRYDELLEKFSASKKEQYVQSLQWLIDSG
                     KIIISESDILLWHE"
     gene            2315..2905
                     /locus_tag="PKU82_00520"
     CDS             2315..2905
                     /locus_tag="PKU82_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOZ81386.1"
                     /translation="MSKLLQSFYLQESAVKVARQLLGKELCTKINGKLTSGIITETEA
                     YMGVEDKASHAWNNRRTQRTEIMYRQGGVAYVYLCYGIHSLFNVVTNIQDVPHAVLIR
                     SIFPKRGFELIKKRRPVNKPLKQIASGPGMVSQALGIDCSMTGTSLLGNKIWIENNDL
                     QLTEKQITTTVRIGVDYAGNDAMLPYRFLINPEILN"
     gene            2905..3615
                     /gene="mtgA"
                     /locus_tag="PKU82_00525"
     CDS             2905..3615
                     /gene="mtgA"
                     /locus_tag="PKU82_00525"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOZ81387.1"
                     /translation="MKNIFIRIRKILWKIIKWFLILSIAGVILFRFVPIPFTPLMIIR
                     FVEQVFDSERDIQFKKDWEPISNISPLLVKAVITSEDQRFNEHFGLDMEAIQKAAQYN
                     EKHKNKKRGASTISQQTAKNVFLFPSRTWLRKGFEVYFTFLIEIFWSKERIMEVYLNV
                     IELGNGVYGAEAASEYYFNKPASQLSVHQAASLAAILPLPLKWSPVKPNARVVRRTEW
                     IKRYMKYAEGLESVNGEK"
     gene            complement(3803..4297)
                     /locus_tag="PKU82_00530"
     CDS             complement(3803..4297)
                     /locus_tag="PKU82_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81388.1"
                     /translation="MTIINHIENKPNWFYIISVSVLTFILPAICFVTEHFFTNTVLSF
                     NLFGKWFIFSAVGLRLFLAGTKQVINPEFTAKEIFHLDSSDSFPILRELGFANLCFGL
                     VAVVSIFKPEWRTVSAFASGLYFGFAAIQHGFNKTSGINEKFALWTDLIIFVFLSVYF
                     IKSI"
     gene            complement(4409..4849)
                     /locus_tag="PKU82_00535"
     CDS             complement(4409..4849)
                     /locus_tag="PKU82_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ81389.1"
                     /translation="MFIIKIIESRKEDINKLQDIFLETRRSTFSWVDTSQFKLSDFEK
                     ETEGEYVLVAMIDEKVIGFISVWVADNFIHHLYVDKSFQDQKVGTKLLNAVLDKFGLP
                     VRLKCEIKNQKAVSFYRQKGFVEIEQGHSEMGVYILFELRKKLS"
     gene            complement(4937..5833)
                     /locus_tag="PKU82_00540"
     CDS             complement(4937..5833)
                     /locus_tag="PKU82_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81390.1"
                     /translation="MMQKIQIFIVIFFLSSCFTFGQDHDSLFARLNGISNNGIDFFNV
                     DGIEITTQKVPADLTPKSISKKFKNLAIKEKEIISTDSTLGFSNFYVSKSKTESGITI
                     NMNYYFFKNSDDKVIGISFIAMNKTDRELEKEFVQLVYDNAIPESVYATLNIGDINFA
                     GRKIPLGSGCRWMGINNVQCPSWGQMNWSVHKTLEDASLSVNNQIAFIKAGNKGKIVS
                     DTTVAVIFEGNETNARKIIYDFKGATSVLVGMSGGKTLTVYFVATPVRNNFVSCVMSF
                     WNNDQINPSGLPPLLEQVMKLK"
     gene            complement(5830..6195)
                     /locus_tag="PKU82_00545"
     CDS             complement(5830..6195)
                     /locus_tag="PKU82_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81391.1"
                     /translation="MRNLVIIILVLFSVSCMTNYPLTTFYVKNTTDKTLNFKASIIKQ
                     SQMGPYEMTLPFMVFPNDSVVARRTNFKKDASPTAWFKKFIIFQTDSLSFNDPKNKDN
                     WIKTTDEKGRPVYTFTMTK"
     gene            complement(6838..6924)
                     /locus_tag="PKU82_00550"
     tRNA            complement(6838..6924)
                     /locus_tag="PKU82_00550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6888..6890),aa:Leu,seq:taa)
     gene            complement(7023..7095)
                     /locus_tag="PKU82_00555"
     tRNA            complement(7023..7095)
                     /locus_tag="PKU82_00555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7060..7062),aa:Gly,seq:gcc)
     gene            complement(7183..9768)
                     /gene="mutS"
                     /locus_tag="PKU82_00560"
     CDS             complement(7183..9768)
                     /gene="mutS"
                     /locus_tag="PKU82_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOZ81392.1"
                     /translation="MQQYNAIKAKYPDALLLFRVGDFYETFSEDAVKTSKILGIVLTK
                     RSNGAASEMELAGFPYHALDTYLPKLVRAGQRVAICDQLEDPKTTKTIVKRGVTELVS
                     PGVTYNEKILENTHNNFLAVVHLTEDVSGVAFADVSTGTFFTARGKDAYIEKLLQNFK
                     PSEVVLQKSMQKDFIARFGDGFYIYTLDQWAFQKEYAEEVLLKHFQTNSLKGFGIHEM
                     DEMIVACGVALHYFGQMQQQTLSHITGIARIDEEQYVWLDKFTIRNLELVASPHENAR
                     TLVDVIDRTVTPMGSRLLKRWILLPLKDIQTIHERHDAVAAFTLDETLRGQIATSLRH
                     VGDIERMLSKIALRRISPREMVQLKRALSQIQPVKDNLATAGNSTFNVLSEQLNPCNL
                     LIQKIQDALIEDAPPLASKGGFIAKGFSQQLDDLRQVAFSGKDYLLKIQQREVEQTGI
                     SSLKIAFNNVFGYYIEVTNAHKSKVPESWIRKQTLVNAERYITPELKEYEDKILGAEE
                     KISTIENEIFQELISFTQNYIQPLQVNASVLSQLDCLLSFANVAIENNYTRPVVNDSL
                     VLSITEGRHPVLEKQMPPGEAYIPNSIFLDQQTQQIIMITGPNMSGKSALLRQTALIV
                     LMAQTGSFVPAKSAEIGWVDKLFTRVGASDNISSGESTFMVEMNETASILNNITQRSL
                     VLLDEIGRGTSTYDGISIAWSIADYIHESKNARAKTLFATHYHELNEMTNSYNRIKNY
                     NVAIKEAGNKILFLRKLVPGGSEHSFGIHVARMAGMPPHVINKAEKMLALLEKAHSNT
                     ELLRNAGHQADKDAMQLSFFQLDDPLLEQIRDEILKTDVNTLTPVEALFKLNEIKTLL
                     SKNKK"
     gene            9817..10347
                     /locus_tag="PKU82_00565"
     CDS             9817..10347
                     /locus_tag="PKU82_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOZ81393.1"
                     /translation="MKKLTGSEMGRTSTEGFKQIQKIPVVIILENIRSALNTGSVFRT
                     CDAFPVEKIILTGITAQPPHKDILKTALGATESVNWQYYSDIKVCISKLKANGYKIFA
                     AEQTSQSIAMPDFKPEKDEKLAVVFGNEVDGVSNETIAMCDGAIELPQHGTKHSLNIA
                     VCAGIMIYDLFQKMKA"
     gene            complement(10394..11848)
                     /locus_tag="PKU82_00570"
     CDS             complement(10394..11848)
                     /locus_tag="PKU82_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ81394.1"
                     /translation="MKVKTLLVSAFLISATQFAFAQESARYGLPKNKDFDKWSAGLTV
                     GLNWFQGDLNEKTGNNNSILNNIQDPVFGLKVGYQISHSVQVNVRGNYTNLSGKKNKT
                     TFRVPNAVTSPQITRNIKFKSPIYQASLNLNYTIGNISFLQRNKRLHFYGEIGLGIFS
                     YAPKVTDLDNGTVYVKKGSVAEGFLPLSLGFKYQIKRFDVGLLATFNKTLNDKVDQVY
                     DSKTESDNWSFFLLGVNYTFGKKQAMMEWVNPMEVVYNDLSDMKDRIDVMSGDKDKDG
                     VSDLFDKDNSTPEGVKVYGDGTALDSDGDGVPDNVDSDPYSNKGAKVDASGLEVDTDG
                     DGVPDSRDMEPNTPKGNLVNFQGMTIAPAGSYGNDGITSNSATGWLPSIFFDVDQSAI
                     KSVYNDRLNTVARVMIKNPKLKLSVVGNCDVDASEQYNDKLGMKRAEAAINHLVKVYG
                     IDASRLTATSLGKKEQLAKGNKRMNRRVDFEVAK"
     gene            complement(11965..12582)
                     /locus_tag="PKU82_00575"
     CDS             complement(11965..12582)
                     /locus_tag="PKU82_00575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOZ81395.1"
                     /translation="MYRNRFLTIEGNIGAGKTSLAKMIAEEFNYHLILEEFAENTFLQ
                     QFYDSPEKFAFPLEMSFMAARYKQLKTFFNKNTLPFPIVSDYMFQKTLLFAKSNLKKN
                     EYGLFEQFFQMLNVGLPLPQLIVYLQKEIPSLQKNIKKRGREYEQNMKDDYLLKINNA
                     YSRFMKKSNTASNILIINTEGLDFVRKRSDFQKIVETIEKTGFRF"
     gene            complement(12569..13078)
                     /gene="folK"
                     /locus_tag="PKU82_00580"
     CDS             complement(12569..13078)
                     /gene="folK"
                     /locus_tag="PKU82_00580"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOZ81396.1"
                     /translation="MALHQNTAIILAGSNLGNRLHNLTEAALFLQRLSTKKMASSSVY
                     ESAPWGNQNQPPFLNQAFSIYNAFTAHELLDVLLTFEKSQGRTRKHQWEPRSIDLDIL
                     FFNNEIICTPELTVPHKHLHERRFVLEPLAELISDFIHPVFKKSISELLVECKDDLKV
                     SKYPLYVQK"
     gene            13136..14893
                     /gene="sppA"
                     /locus_tag="PKU82_00585"
     CDS             13136..14893
                     /gene="sppA"
                     /locus_tag="PKU82_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOZ81397.1"
                     /translation="MKQFLKYVLATLTGLILFCLLTFFLFAVMLGSAFKKEVVEVKSN
                     SVLKIEFKMPIAERSDNNPFRNLNLSTLKASKKPGLNDIVANIEKAEKDDNIKGILLN
                     LNYFEAGYATTTEIRNALVKFKKSGKFIFAYAEYYSTKAYYLASVADKIYVHPEGAVD
                     FKGLFTQMMFMKGALDKLEIEPEIIRHGKFKSAIEPFILDKMSPENKEQVATFINSIW
                     ETISSEIAESRKISREELEQIADSLSAENAKDALRLKLVDQLCYKDELEKILAEKSGV
                     NDDDINYISLNKYNEAVVKSDKKFTTDRIAVVYAVGDIVSGSGEADQVGSDRIAEAIK
                     KARLDDKVKAIVLRVNSPGGSALASDVIWRETKLAKEKKPFIVSMGNLAASGGYYISC
                     LADTIVAQPTTITGSIGVFGLLFNAQKLLNNKLGLTFDTYKTGPYADLGMPTRPLEPA
                     ERNILQRSVEDVYSTFIGRVAEGRKMETVMVDSIGQGRVWSATDAKRIGLVDIYGGLN
                     DAVTLAAKKAGLDNYRIKELPEQKDPIQEILEDMKGESESMMMPDYMKESMKLMKYLK
                     QISEQDKIQARMPFGIAVE"
     gene            complement(14945..16546)
                     /locus_tag="PKU82_00590"
     CDS             complement(14945..16546)
                     /locus_tag="PKU82_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ81398.1"
                     /translation="MAKKILVVDDEPDLELLVKQRFRSQIRNNELQFDFAHNGEEALQ
                     KLKDDVDIDLIFTDINMPVMDGLTLLNQIKENKIQPKAVVISAYGDMDNIRKAMNSGA
                     FDFITKPIDMTDLDTTLRKGLSEIEIIKKGQKAQEALQQTIIEKEIAVLEKDKAEESK
                     RLKQQFLANMSHEIRTPMNAIIGTVNLLHKSNLDDKQSKYIGIIKTSADNLLVIINDI
                     LDISKIESGKFTIEEIPFNLHEKVNHVYESLKVKAEEKGLAFNVDYCSTLSDNYIGDP
                     VRITQVLINLTGNAIKFTEKGSVTIRVASIADVPNGVSISVVDTGIGIAQDKIQHIFE
                     SFTQESSDTTRKFGGTGLGLAISKQLIELMHGELVVESTQGKGSSFIMKLPLVATNEL
                     ATETKDKVSSSVEIRKGLRILLAEDNQFNQMVAEDTLRMVIPDLTLDIVDNGVQAIEK
                     LTSGNYDLVILDIHMPEMDGYETTQFIRTQMPSPLNGVPIMAMTANVISEEIDKCFAV
                     GMNAYVSKPFTHEDLFDKIKSLTSK"
     gene            complement(16549..16932)
                     /locus_tag="PKU82_00595"
     CDS             complement(16549..16932)
                     /locus_tag="PKU82_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ81399.1"
                     /translation="MQTKILIVDDERDVELLFRLKFRREIKEKHIDLHFAFSGEDALN
                     YLHNINPMDVLLVLSDINMPGMNGLQLVEKVKAEFPAMRIIMVSAYGDDKNYNEAKAK
                     GVDDFVTKPVDFEDLKRKMARVITI"
     gene            complement(16943..20287)
                     /locus_tag="PKU82_00600"
     CDS             complement(16943..20287)
                     /locus_tag="PKU82_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ81400.1"
                     /translation="MPSKAIQKVTRLFWLFLLIAINAFAQKSDVFRHLNTVNGLSHNR
                     ITCIIQDKIGFIWIGTEDGLNRYNGYKIDVYKHNPSDETSVSSNGIRCLFEDSKGKLW
                     IGTDDGLSFFDIETEHFGSFRTNTKGNSISSNQITCMEEDASGVLWIGTAGGGINLFN
                     PSTGKWQNFKSDKSNPNSICSNNITAIVKDKNGVLYLGTDNGLAIADRNQPGIFTIID
                     HDPANPNSPLENSITCLYADFQNKIWIGTLNSGMSAMRTDNRSFTHYTNLGKYANNSV
                     FGINKDLDGSIIAGTIGGGLIIFNSDASTYSSFTHSKTDPFSISSNSIWTIYRDKAGI
                     IWIGTDNGLDYYKEDLMRFKTELPLPDDPLSNATPNKNVFCFLPEGNNLWMGVLGTGV
                     VVKESTGTIKQVYNAANGLADNSVFCLIKDRNGIVWVGTYNGLTSINPQSGVVRSYRI
                     ADGLTNSNIRSLAEDENGNIYIGTYGGGLCVLNRQTDKISSIRKEDGKGLTNNIITAL
                     KYDGNGNLFIGTYGGGLCLLNISANTITAFESKPGSNTTISSNYVNSIYSFSNQQLLV
                     GTYSGGINIFNYATKSFTALTETNGVPNNNVTNIVSDAEKNIWFTTGNQICKMKLAPS
                     DSILTMRIYDEQDGVFNRFNPGAFAQTADGKIYFGGISGINRFLPSAIRDNPYIPPVV
                     ITKFYLFEKPYFMDTIIMSKNMIELTYNQNFFSFEYAALNYILPEKNQYAYMLEGLEK
                     TWTYCGSKRDRQYTDLDPGTYKFRVKACNNDGIWNQDGTYIIIIIKPPFWKTWWFYIL
                     SGLTFVGLTITYIRWRTRTLMKQYEILEQKVQERTAELREEKEKSEQKSLEIERALKE
                     LKDTQSQLIHAEKMASLGQLTAGIAHEIQNPLNFVNNFSELSQDLIDELNATDDKEEW
                     KSISHDIKTNLEKINEHGKRAERIVKSMLMHSRSQTAEKVLTDINKLAEDVMNLAYTS
                     LRANDSNFGCKNMIELDASLPKLSIVQQDISRVLLNIFNNAFYAVNQLRKKSDKAFHP
                     EVRLRTWKAANKVYISISDNGGGIPDDIVDKIFQPFFTTKPTGEGTGLGLSLSYDIIT
                     KGHYGMLSVNVQKGIGSEFVIELPQL"
     gene            complement(20305..21462)
                     /locus_tag="PKU82_00605"
     CDS             complement(20305..21462)
                     /locus_tag="PKU82_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81401.1"
                     /translation="MPLTVFSQSDTLNRATNIVGCQSGDCVNGIGVYLYKNGNRYEGA
                     FKNGSPDGKGIMNYRNGNRYEGDFTQGRLNGKGNYNYRNGNSYTGDFVNDQFHGRGIY
                     IYNSGNQYIGDFFEGSKHGSGTFTFNDGSTYTGQFVDDMVQGKGTWRYANGDWYEGDF
                     VNSKKSGKGTYYYNDSSRYSGDFVNDVYNGLGVFTFADGSTYIGQFRNDLFNGQGTCY
                     YSNGDVYTGEFRDGLYSGGGVYAYATGDRYKGTFLNNVFNGEGTYIDESGEETVTMYE
                     NGRAVGESKVIDKENVKPVPEENKTEAKKPEDKKEEPKVSEPTVAKIPCPACNGKGKI
                     LQAEVKRNRSVVKDISNGLGPRNFVTYTVSEVVKPAAWATCKVCHGTGIKQ"
     gene            complement(21479..23644)
                     /locus_tag="PKU82_00610"
     CDS             complement(21479..23644)
                     /locus_tag="PKU82_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ81402.1"
                     /translation="MKKQVLIFIFCLIGTFVRGDAKHLYSVFDRNDLPVFSRPFFITI
                     TADPIALKSAERMLGDAIKSNRQQEIFQNANNAGYYNLLNGDFLQALKFLQIAVNHKP
                     LSKEAAPTMAQYAFALDVAGRHEQAFSIYKELLEKDLANSTQIRAYVQAMAGLIFLQH
                     ADTVSAKPLLGNALQYFSANNFLLQQYYLYERMGDINLVAKNFNNSSANYLQQLKYAT
                     KLNNKTFQALATRNLGLCNLKRDDYLKAVSYFRQSLDFRDNVLLQKLLKDTYLKIVTI
                     SSFNNDFEKADRYHELYRKLKANETVVKETAALQAEKEKILFLLSQSANNENNMVSRQ
                     EYELSQQLTTLDIERQNKEKALEELTLAEAQKKLKELELDKIAGEKLKQEAELAKKEL
                     QISRQKEFRNILLGISGLIVLGIVFLINRYRLKRKSLEQLRQAHQELRQAHEQLKQTQ
                     EQLVQSEKMASLGQLTAGIAHEIQNPLNFVNNFSTLSMDMIDDYLKSKDETLLDELKS
                     NLKRINHHGGRVSSIVKGMLLHSRNKTAEKELSDINLLVDESLALAFHGKKSTEADFY
                     CHIEKDFDATAPPVKVLPQELRRVIINLINNAFYAVHERAVKSNANKEEVKYKPQVSV
                     TTHVAGQFLEITITDNGTGIPDTIKDKIYNPFFTSKPTGKGTGLGLSVSFDIIEKQHG
                     GKLKFESEQGVGTVFTISLPLNAPPDLKQSNGDVKKSVV"
     gene            complement(23704..26160)
                     /locus_tag="PKU82_00615"
     CDS             complement(23704..26160)
                     /locus_tag="PKU82_00615"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOZ81403.1"
                     /translation="MRKFFYLVLLGSIFSTTVFSQGIVSGVVKDKNHKPVAYANVYVK
                     PGFVGTTTSSDGIFEIELVPGKYTLYCQNLGYKQFSKQIEVGAGKQQIEIELTDELYS
                     LKEVAVTNNKEDPAYEYIRKTIAKRKSYLNEVDAYSCNVYIKGVQKLNKYPEKFLGKV
                     VDFGGVVDSTTGIFYLSESVSKYSFEKPNKIREEMISSRISGNNRAFSFNKASDMQFN
                     FYENRVMSEGISSKGFVSPVAGDALFYYRYKLIGSVVENDRLIHKIEVIPRRQNDPVF
                     RGVIYIADDLWRFQFVDLFLTRDAGIEFLDTLRIRQSFVPVENNVWMPVTNLFDFSFR
                     ILGFQGGGNYIGYFSDFKINPGFPKKTFSAEILKISPDANKKDSAYWDTVRPIPLTID
                     ETSDYFKKDSLAIIRESKQYKDSMDRINNKFSFFNFLLAGYSYRHSYREMTYTIKPIA
                     NTFSFNTVEGWNVALNTEFVKRLPNRKRLTVFTNTRYGFSNTHWNANAGFKYLFNRFS
                     DNTISVEGGTAVNELNSTSPVKPLVNMLYTLWAERNYLKIYEKQFFKIAYGREWFNGF
                     NATAEIEYGRRNTLKNTTDYTWKNFKNREYTSNNPINPDKDIKNFRPHHYFNTHLSLT
                     YSPFTKFITRPDGKVREESGWPVFFVNINKAFGNVINSKTDYLSFDAGFNHQIDFGML
                     GYVVYNCRYYRFVNRKDVEFPDFFHFAGNKTIVSDFKAEDFAYLDYYKYSNMKSAFAI
                     HAEYNLNRFITNKIPLIRKLKLNEVVGFHFLKVSEMKSYYELTVGLEKLGVIRADFVC
                     SYDGKASPFYSFRFGLKLRQ"
     gene            complement(26216..27142)
                     /locus_tag="PKU82_00620"
     CDS             complement(26216..27142)
                     /locus_tag="PKU82_00620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOZ81404.1"
                     /translation="MVSISVVVPTYNRLNFVVKTIESLLNQQSDDFEILIVDDGSTDK
                     TEEYFSTYKHPKVTYHRIVNSERGFARNYGASIAKGRFINFFDSDDLAYNNHISTALN
                     YIAKFDNPEIFHLNYVYQTLDGQDLDVLKADSDNIGEMLIKKGNILSCNSVVIRKDIA
                     EKFPFCESRLLSGTEDYALWLALSSRFKIHYVPEITSVVIDHEQRSMASHDIIKMINR
                     NMFLLDHLQADQHFMQYVGKRFNQIQCECFSFIALHLMLEGKNKDGIYFLSKALIADW
                     RFPFRRIRFMAIMKHMLKSFFRIQRSKVPEAV"
     gene            27275..27844
                     /locus_tag="PKU82_00625"
     CDS             27275..27844
                     /locus_tag="PKU82_00625"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOZ81405.1"
                     /translation="MNHRPTEFTERKKGAIEVICGSMFSGKTEELIRRLKRARIAKLK
                     VEIFKPSVDQRYHESNIVSHDSNYIPSSPVQSSSQILLLTNDVDVVGIDEAQFFDLEL
                     PNVCLKLAHQGIRVIIAGLDMDFKGEPFGPIPRLMAIADFVTKVHAICMDCGDLALFS
                     YRTVPDESLIVLGEKESYIPLCRTCYHKR"
     gene            27904..30405
                     /locus_tag="PKU82_00630"
     CDS             27904..30405
                     /locus_tag="PKU82_00630"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOZ81406.1"
                     /translation="MYTITEAASILKGTLVLNVNDNQTFRYLLTDSRQSGVAGESLFF
                     AIKGDRHDGHLFINDLIRKGVKNFVVSDTHTSQNKEANFIVVENTLDALQQLASFHRN
                     KFNLKTIGITGSNGKTIIKEWLYQLLQPDFSIVRSPKSFNSQTGVPLSVWQINNEHNL
                     GIFEAGISIPGEMQKLEKIIQPEIGIFSNVGEAHSENFSSLDVKIQEKLLLFQHCKTL
                     IYCKDYSGIHNAVIQSGYLENGLKCFSWSRRIKSDLQIGRIEKQNNSSTIQGIFQQKF
                     IRIEIPFTDDASIENAIHCWAMLLFLEIEQETIAERMMLLSPVAMRLEMKQGINNCSI
                     INDSYNSDLGSLQVALDFLNRFQQQNNTGAINSGLRKTVILSDILQSGRNEDQLYGEV
                     AEMIHSKKVDRLIGIGPAISRHTDLFKTEKVFFSTTEKFIEQYSPGSFINEVILLKGA
                     RVYGFEQISRLLQQKGHETVLEINLTSMIHNYNFYKAKLKPETKIMCMVKAFAYGSGS
                     YEVANTLQFHRVDYLAVAYADEGIELRKNGITVPIMVMNPQEQSFDKMIEYHLEPEIY
                     SFRIMSKFSESVKRIRTYNQSPFPIHLKMDTGMHRLGFEEDDLNELVVRLKNSKHLKV
                     ESIFTHLAGTDEAALDFFTQEQFQRFKDMSDAIKKHLQHNVLEHVLNSSGISRFPEMQ
                     LSMVRLGIGLHGIGTPSEQRQLLNVASLKSTISQIRSVKKGESVGYNRKAILDSDKII
                     ATIGIGYADGINRKMGNGKVSFLVNGKLAPTVGNICMDMCMLDITNCQAREGDEVIIF
                     GNGLPVAELAAKTETIPYEILSVISQRVKRIYFQE"
     gene            complement(30406..31677)
                     /locus_tag="PKU82_00635"
     CDS             complement(30406..31677)
                     /locus_tag="PKU82_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ81407.1"
                     /translation="MLRRVLIFLLIVFLIDLYVFQGIKVLIKDLTPSMSRLISGIYWF
                     FTVFTFSVFIAGLITDWHTWPKAVRVYLFAAVMIILITKLFVVLFLLIDDLTRLVRWG
                     FSGLYQRFYDSPQIVNPEEKIVVSRSQFLVRMAFVISSIPFISLLYGMGDGKYNYQVK
                     RRKIKFPKLPSTFEGFRIVQISDIHTGSFFDTKHIEKAVSMINTLKGDVIFFTGDLVN
                     DRSDEAVPFIDTLSKLNAKHGVFSILGNHDYGDYVQWPSREAKVKNLEQLKNIHKEMG
                     WKLMLDEHAFISEGNDKLGLIGVQNWSTHMRFPKYGSLPKAVQGMEFAPVNILLTHDP
                     SHWHGEVTENYPQIDLTLAGHTHGFQFGVEIPALKIKWSPVQYVYKEWADLYDDVNGQ
                     HLYVNRGLGFLGYPGRVGILPEITVIELSKG"
     gene            31753..33801
                     /locus_tag="PKU82_00640"
     CDS             31753..33801
                     /locus_tag="PKU82_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ81408.1"
                     /translation="MKDTAQPTNKLIHEDSLYLQQHAHNPVHWLPWSVDAFEKAKTEN
                     KLLIISIGYSSCHWCHVMEKESFENTTIAEVMNENFICIKVDREERPDIDAVYMTAVQ
                     LMKGQGGWPLNCIALPDGRAIYGGTYFNPQQWLQVLEQLSMMYEQDNSKFLEYASSLQ
                     EGMLQLEKIETTNTKPDFDILKNSISKWSSLFDKIYGGLQRAPKFAMPVNLNFLMQYG
                     HVCNDVEVQNHVRLTLNKMYNGGIFDQLDGGFARYSTDSRWEIPHFEKMLYDNAQLIS
                     VYAQAFRAYNNISFKNAAEETINFLNEKMNDKKGFYYSAIDADSEGEEGKFYFWTKAE
                     IRATINQLHLENISKENAFIIACHYFSIPDSKKENDEIVLQCNTAIFNDSSVQMIEPN
                     QQKELIDKIKSAMKMARSERIHPVCDKKLITSWNAMMISALADAALYFNIKEYELQAI
                     TTFNYLEQNLFIEEGRLLHSSGTKQNSNLHLEDYALLTQACIKLFECKWDFQWIEKAI
                     SLTGYAIQYFSHDDNVLFYDYTANDSVLQFRKTELQDNVIPCANSVMANNLFLLSKYL
                     NNENYLLRSKKMLEKVIDQIPNYGFGYANWALLLQRINQPFYEVAFGGYNALKNKNKL
                     NEYFLPMKITAPIILKDKIEFTADKNETDDLIYVCRNHSCLLPTSDIIKVAQTISDQQ
                     "
     gene            33798..34238
                     /locus_tag="PKU82_00645"
     CDS             33798..34238
                     /locus_tag="PKU82_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81409.1"
                     /translation="MKQNVFLLCVILNITITGCQIFKINKSDKHGNRQGHWEHYWNDE
                     GTIQRKGKYKHGLEVKKWKYYDLNGKLIKQEKYSKKRDVINIKTYYDNKKIKSNGKAF
                     LINENDSILHFYWQGPWNYYSPTGVLEKTEWYEKGKIKKAVTYP"
     gene            complement(34264..34758)
                     /locus_tag="PKU82_00650"
     CDS             complement(34264..34758)
                     /locus_tag="PKU82_00650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ81410.1"
                     /translation="MAEETLNMTATTFENLSQPNAAMDVFPINYSEKHIAELIHEIRR
                     RVFVLEQHIDPMIEFDTFEHESQHYLAYVKNIPAGTARWRHTDEGIKLERFSVLKEHR
                     NSGLGRMLVNRLLNDVKPIGKKVYLHAQTTTVRFYEKAGFKVVSELFYEANIPHFKME
                     LVIS"
     gene            complement(34770..39068)
                     /locus_tag="PKU82_00655"
     CDS             complement(34770..39068)
                     /locus_tag="PKU82_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81411.1"
                     /translation="MSRISYLILMLFFVNVSVNAAVTVSPATSGNCLNVTPSAFLPIG
                     NIVITEGANGDFAIQSNKTLILSAPTGFMFQPGSGNVSFLASRNITTASIAVTLTTIT
                     VTISVSGIPKADELIISNIYARASTSNVSGNILRKSPGGGTATIAGDVAGAGINHGTL
                     SSAGTGQIVTSVANGAWSNPATWQGGKIPLCTDNVIIYHQINVDVVTPSLASLIIKSG
                     AKLTANLPVTVNSNLTIEANAWYVHNNSSNAATTIFNGTEAIDDNATIEVNNWSAATV
                     PFMSGVSSTVGNLILNYNAVWQQDGLFAPSKIKGNVSVTNGQVVMDDGTGMSTLLNLN
                     HVSIYKTGAIIFAQGANRNLTLNTLSFTDSSKSNAITALMFRTYGTLLWNNAGSVYLS
                     HDFSAVEGTTGYNAFNATVSIGGDLNVVKGKVDFFRYVDGNGVIQVAGNTYCNLTAGN
                     WFRLADYNTINLDFTTNNFSISGTESCYLQGSDGSTNFNINGSFSYTASSLFVFSNNF
                     TNNALQRIDVLKDFSVSNGNVGLAFTSGKLNTHISGNLNVSGSSTQLVSQFYPYGTDS
                     VKFVIDGNCSLNGGMFNVSWNRGPVSFISSGIFNQVNSNFGVVFHPFPGNYAACSVFI
                     DSLNFSGGTFKVFDSYITDGKTVSITIGSDVNINFQNSSDRFVLISNTGWWNNPELNL
                     VINGDLKISGNSSAIFASNVGRGEENIVINGDFEIQGGNNAIGGSTSLGSQQHDISVL
                     INGNMTQTGGQFYYSVNEGNVDYNIQNNLIQTGGTQNLTYQDAAAKLNIGGQYLQSGG
                     TFNFHASTGNTSLSDSVFVQGNFSQSNGVLRFDNAQGSSNISQNVLILNSDSILLSGN
                     GIITHANHLTTNTVFGKILFNKNGISTFQRATSTHDLQQVEMVVGSLTTLDVKSSIQP
                     LLIASHVSSAPAVNNVFTINGVLDMGLQRIMARQQGNYYSQLSVGATGKIKTMNPNGF
                     YSTSAASTINTLIAGNNRMNFWLDAQSIIEYNGTQNQNITGIPNGIVNAVSNKYGKLQ
                     INFAGVNNINFVKPEADSVVFVRSGLMLTSGELNLNADHIANTNGLSIVLENGCQILH
                     ASGYIRSETIDGSARIHLSSQQSVNYEIPFGFNSTTYIPFKYQCSSGCDWVSLATYRS
                     LPDNTPFPPGVTHVRSISGADNSLQTIDRYWYVRAVGNPNANLRFTYAPIEKGVISTP
                     RAQRWIGNDAWESFKGSQSNPSPFETDVNGITGLNSWWTLSSQSSPLPVEILSFGANC
                     QSQSVILKWSVATQLNNDIFILERSRDGKKWENLTSIKGAGTTNTLMNYETKDEAPYS
                     EVTYYRLAQIDYDGKRNELKTIAVKGCGSDPIRILYYSFSDHKTVIKIQSSYNDKIEI
                     FLFNENGQTVARKVQNISNNIQEIEMDCPQLNTGIYMLSIRGSFNNIIKKLVKN"
     gene            complement(39155..40078)
                     /locus_tag="PKU82_00660"
     CDS             complement(39155..40078)
                     /locus_tag="PKU82_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOZ81412.1"
                     /translation="MPVNHQEVQSLSHLELLAKQVVEGFITGLHKSPFHGFSVEFAEH
                     RLYNTGESTRHIDWKLFGRTDKLFVKRFEEETNLRCHIVIDNSASMHYPEVKGGKSLQ
                     NKITFSVYSAAAILYMMKQQRDAIGLTVFSEQIDVQTQAKSSTAHHKLVFAHLEKLLE
                     NKTDVNRKTATADALHQLAESIHKRSLIIIFSDMFENIDSNEALFKSLQHLKHNKHEV
                     ILFHVTDKKHELDFQFENRPYRFVDVETGEEIKVHSNLVREKYIEAMKEYKSMLVHKC
                     AQYKIDFVQADIHEGYHQVLLAYLLKREKLH"
     gene            complement(40102..40422)
                     /gene="trxA"
                     /locus_tag="PKU82_00665"
     CDS             complement(40102..40422)
                     /gene="trxA"
                     /locus_tag="PKU82_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOZ81413.1"
                     /translation="MAIEITDSNFEEVVMKSDKPVLVDFWAEWCGPCRMVGPIVEELA
                     KDYEGKAVIGKIDVDSNPNVTMQFGVRNIPTILFFKNGQVVDKQVGAAPKNILAKKID
                     TQLV"
     gene            complement(40580..44170)
                     /gene="dnaE"
                     /locus_tag="PKU82_00670"
     CDS             complement(40580..44170)
                     /gene="dnaE"
                     /locus_tag="PKU82_00670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOZ81414.1"
                     /translation="MPRFSHLHNHTQFSLLDGAADIKSLYKKAVKDNMPALAITDHGN
                     MFGVFNFVAEAAKYNTSENPNLIKPIVGCEFYLVENRHKRSFTKDDKDVRYHQLFLAK
                     NTIGYKNLVKLTSLGYMEGLYGKYPRIDKELVLKYHEGLIATTCCLGASVPKTILRKG
                     EAEGEKEFLWWLDIFGEDYYVELQRHDIPEQNTVNEVLIGFALKHNVKIIASNDSHYV
                     NREDYNAHDILLCINTGEKQSTEKFKDGDEDTSARDKRFAFYNDEFYFKDTSEMQQLF
                     SDRPEAIDNTNEIVDKIEPLKLKQDILLPHYQIPKGFESQDDYLKHLAWEGARKRYSE
                     ITPDIEERINFELFTVKTMGFAGYFLIVADFINHGKNIGVLVGPGRGSAAGSAVAYCI
                     GITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVVDKYGKNQVAQIIT
                     YGTMAAKMSIKDVARVLDLPLPDSNALAKLVPDRPGISLGRLLTAPIDGEKSLKTKEN
                     LSADELERVKSLRSILEEGTSLQSSVLKEAMVLEGSVRNTGIHAAGIIIAPTDLTDII
                     PVSTSKETDLLITQFEGKIIEDAGVIKMDFLGLKTLTILRDAVRLIEKNHGVKINLDE
                     IPLNDIRTLELYQRGETNGTFQFESAGMQKHLKDLKPDMLEDLIAMNALYRPGPMEYI
                     PNYIARKHGKEKVTYDLPEMEEHLKETYGITVYQEQVMLLSQKLGNFTKGDADTLRKA
                     MGKKQIEVLNKMKSKFMEGAKSNGHDEKILEKIWTDWEAFASYAFNKSHSTCYAFVAF
                     QTAYLKANYPSEYMAAVLTNNLNNLDKITFFMDECRRMNIPVLGPDVNESELQFSVNK
                     KGEIRFGLAAIKGVGEAAAESLLSERKKGGVFTDVYDLVSRVNLRAANKKCFENLALA
                     GALDFDTSFHRAQYFAIDPKDNQTQLEKIIRFGSANQESKSSTQHTLFGDLGGVDASK
                     PKIIPTEHFSNLEQLKKEKEIIGIYLSGHPLDDYKEEIKTFCNISVEQLNELKLLNNR
                     DVNFAGIITNANHRVSKTGKPFGSFTIEDYNASFEFAVFSEEYAKFKHLLNNDAFVFI
                     KARVQTKFYNEKEFELKINSVHFLDEVANKMAKGIMISFRSESINEMNIKKLKEISKN
                     SKGKLTVKFSIRDNDQNLSIDVESNQLKLEYNKELFKELNSISERVSLIK"
     gene            complement(44473..>45328)
                     /locus_tag="PKU82_00675"
     CDS             complement(44473..>45328)
                     /locus_tag="PKU82_00675"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOZ81415.1"
                     /translation="LIAIGGNEDKGTESEPNFEQKNNLNFFALQILSRICQEMGGKNA
                     KIEVITSASSIPVEVGENYIDAFGKLGCTNVNVMDIRNREDATKSEYIERIKKCDGVM
                     MTGGNQLRLSTIFGGTEILEIIQHRYQHENFVIAGTSAGAMAMSNTMIYQGSSSGALI
                     KGEVKITTGLALMGDVIIDSHFDKRGRFGRLAQAVASNPSCIGIGLGEDTGVLLTEGH
                     LLEIIGSGLVILIDGHQIRHNNIADLQDGVPISIENLIVHVLTKGNCYDLKSRKFYSE
                     LVKESKPA"
BASE COUNT        14357 a         8571 c         7793 g        14607 t
ORIGIN      
        1 gcccatgcta cctcaacacc tgcaggcgat ggcattgaag caaaagtgat ctcacatgtt
       61 ttaggaaaca aagttcctgt aagctctacc aaatcaatga caggacatga gtgttggatg
      121 gcaggtgcca gcgaggtagt atattctatg ctgatgatgc agaatgattt tgtggctcca
      181 aacattaatt ttgaagagcc tgatgaagat actaaagcca tcaacatcat cacttcttca
      241 atggaacatc gtatcaactg ttttctttct aattcatttg ggtttggtgg tacaaacagt
      301 gccttgatta tacagcgatt taaaagctaa cagcaatttt cccaaaacca ctattttatt
      361 ttacgcgggc agcataattc gcaattctaa ttttacattt gtaacatgag cgttggtgac
      421 aaactgcatg agattttaat aaaatactgg gggcatagtc gtttcagacc actgcagcag
      481 gaaattattg aatctgtagt tggtggccat gacactttag cgcttatgcc aacgggaggc
      541 ggtaaatcta tctgttttca ggtgccggga atttatctcg atggaatatg tgttgtgatt
      601 tcacctttga tttcactcat gaaagatcag gtggataacc tgcgtgccag aggaattaaa
      661 gcgtctgctg tagtttcagg aatgagcaag acagaaattg aaattgcctt tgacaattgc
      721 atattcggaa agtcgaaatt tttatatctg tcacctgaac gtttaactag cagtctggcc
      781 ttgacaaaat tacgagagat aaaaataaat ctcattgccg ttgatgaagc acattgtatt
      841 tcgcaatggg gttatgattt tcgcccatcc tatctgcgta ttgctgaatt aagagaagag
      901 tttcctgaag ttcctgtaat tgcactcact gcctctgcaa caaagtctgt aaggaatgat
      961 attatcgaaa aactccattt caaaaatcac aatttttttc agaaaagttt tgagcgcaaa
     1021 aatctgagct atattgttga atacaacaaa gacaaactaa cacgtactgc tgaaatttta
     1081 aaatcggtta aaggaagtgc tgtcgtttat gttaacagca gaggtaaaac aaaagacata
     1141 acaacttatc tgaacagcca gggcatcaat gcagattttt atcatgcagg gctatctaca
     1201 gccgatagaa acaaaaaaca aattgactgg atgcagaata aaatacaagc tattgtagct
     1261 accaatgcat tcggcatggg tatagacaaa gctgatgtgc gtacggttat acatgcagat
     1321 ttacctgata gtcctgaagc atattatcag gaggctggtc gtgcaggacg tgatgaaaaa
     1381 aatgcttttg ctgttttaat ttacaacagt gccgacaaag cagacttaga attgcgtttt
     1441 caaaatgcat ttcccgaaat aaaacttatc agacttatat atcatgctct gggaaattat
     1501 ctgaaagttg ctttaggcag tggtgaaaac atcagtttca attttgatgt ttctgatttt
     1561 gcgtcaactt ataacttcaa tcctttggta actattaatg cattaaaaat tttagccgaa
     1621 gcaggttata ttcatctctc tgatgcagtt tatattccat caagattaaa atttacagta
     1681 ggccaagcag aactatacaa ctttcaggtg cagcatccgg agtctgacaa ggttattaaa
     1741 acaatacttc gttcgtatgg tggtagcttc gataatttta tcagaatcaa cgaggcagag
     1801 ttagcaaaaa gattaaacat tgcgcaagat gcactaataa ggcatttgtt ctttttacaa
     1861 aaaaatgagt tgatagaata tctacaacag acagataagc ctcagctaac ctatttaatg
     1921 gcaagggtga aacaggaaac cgttgttcct gatataaaat tattagagtt tcgcaaacaa
     1981 cgttttaatg agagagtgaa tgccatgcta cattatgcag atagcctaac atcatgtcgt
     2041 agtatgatgt tgctaaatta ttttggcgaa actacaaata cacgttgtgg cacctgcgac
     2101 tattgcagaa aaagaaataa aattgaactc aacgacctga cctttgagaa acaaaatgca
     2161 gaaataaaag attttattca acaacaaact ttaagatacg atgaactgct tgaaaaattt
     2221 tctgcaagta aaaaagaaca gtatgttcaa tccctgcaat ggctgattga ctcaggaaaa
     2281 ataattattt cggaaagcga tatacttttg tggcatgagt aagttgcttc aatcttttta
     2341 tttacaggaa agtgctgtta aagttgcaag acagttatta ggcaaagaac tttgtaccaa
     2401 gataaacgga aaactaactt ccggcattat tacagagaca gaagcctata tgggtgttga
     2461 agacaaagct tcgcatgcat ggaacaacag acgcacacaa cgaacagaaa tcatgtatcg
     2521 gcagggtggc gttgcctatg tttacctttg ctatggtatt cattcacttt ttaatgttgt
     2581 tacaaacatt caagatgttc cacatgcagt acttataaga agcatctttc caaaaagagg
     2641 ttttgaactc attaaaaaaa gaagacctgt caacaaacca ttaaaacaaa ttgcatcagg
     2701 gccgggaatg gtttcacaag cattgggaat tgattgcagc atgacaggta catctctact
     2761 tggaaataaa atatggattg aaaacaatga cctgcaactc acagaaaaac aaattaccac
     2821 aactgtccgc attggcgttg actatgcagg caatgatgcc atgctgccct atcgtttttt
     2881 aataaatcct gaaatactaa attgatgaaa aacatattta tcagaatcag aaaaatattg
     2941 tggaagatca ttaaatggtt tttaatttta agtattgcag gagtaattct gtttcgtttt
     3001 gttcccatac cttttactcc attgatgatt atccgatttg tggaacaggt ttttgattct
     3061 gaacgtgaca tacagttcaa gaaagactgg gagcctatat caaacatttc accattgttg
     3121 gtaaaagcag taatcaccag cgaagaccag cgctttaacg aacactttgg cttagacatg
     3181 gaggcaattc aaaaagctgc gcaatacaat gaaaaacata aaaacaaaaa acgtggcgcc
     3241 agcaccattt cacaacaaac cgcaaaaaat gtttttcttt ttccatcgcg cacatggctt
     3301 cgtaaaggtt ttgaagttta ctttactttt ctcattgaaa tattctggag taaggaacgc
     3361 atcatggaag tttatttgaa tgtaattgaa ttgggcaatg gtgtttatgg tgcagaggct
     3421 gcatcggaat attattttaa taaaccggca agtcagttaa gtgtacatca ggcagcatcg
     3481 ctggcagcca ttcttccact acctttaaaa tggtcgcctg taaaaccgaa tgcgcgtgtg
     3541 gtgcgaagga ctgaatggat taaacgatat atgaaatatg cagaggggtt ggaaagcgtg
     3601 aatggtgaga agtgatgagg tgataaagtt gaattagatg atcagcctcg gctgacaggt
     3661 ttgaagaatt tgggaattgt ctattttgag atttgttgat tacaagtatt gaatgtagag
     3721 gatatttaaa ggttaagagt ttatggaatt agtaaacaga aactacatta gatgaaaata
     3781 agatggttat gttaagtgac acttaaatac tttttataaa gtaaactgaa agaaaaacaa
     3841 aaataatcag gtcagtccac aaggcaaatt tttcatttat tcctgaagtt ttattaaatc
     3901 catgttgtat ggcagcaaat ccaaagtaaa gtccgcttgc aaatgcgctt acagttcgcc
     3961 actccggttt aaagattgaa acaacagcaa caagtccgaa acataagttg gcaaagccta
     4021 actcacgaag tattggaaaa ctatcggaac tatcaaggtg aaaaatttct tttgctgtaa
     4081 attcaggatt tatgacttgt ttagttcctg ctaaaaacaa acgaagaccg acagcagaaa
     4141 agataaacca cttaccgaac agattaaacg aaagcacagt gttggtgaag aaatgttccg
     4201 ttacaaaaca tattgcaggc aaaataaatg tcagcacgct gacacttatt atgtaaaacc
     4261 aattcggttt gttttctatg tgattaatga tagtcattga aactataaag gtaatatttt
     4321 atttttaaac tatacagata aaaatggaga aggttttaaa atcagttttc ggacaatgca
     4381 ctcagaaaac acacaatcag ggactgcttt agcttaattt tttacgtagc tcaaacaaaa
     4441 tatatactcc catttcagag tgcccctgct ctatttcaac aaaccctttt tgtctgtaaa
     4501 aagaaactgc tttttggttc tttatttcgc atttcaacct gacaggcagc ccaaatttat
     4561 caagcaccgc atttaacaat ttagttccaa ctttttgatc ttgaaaactt ttgtcaacat
     4621 acaaatgatg aataaaatta tcagctaccc aaacagatat aaatccaatt actttttcat
     4681 caatcatagc tactagaaca tactcacctt cggtttcttt ttcaaaatca gacaatttaa
     4741 attgagaagt atcaacccaa gaaaatgtag agcgtcttgt ttctaaaaaa atatcttgca
     4801 atttattgat atcttccttt ctgctttcaa taattttaat aatgaacata tttaaaaaat
     4861 aactgccaga gatgttggtc atcataagaa gccggagccg atatgactat gagttatgat
     4921 tgtatttact acattgtcat tttagtttca tcacttgttc taatagtggt gggagtccac
     4981 ttggattaat ttggtcattg ttccaaaaac tcattacgca actaacaaaa ttatttctca
     5041 ccggtgtggc aacaaaatat actgtcaatg ttttacctcc actcatgcca actaaaactg
     5101 aagttgcacc tttaaaatca tagataatct ttcttgcgtt ggtttcattt ccttcaaaaa
     5161 ttactgcaac agttgtatct gaaacaatct tgcccttatt tcctgctttg ataaaagcta
     5221 tctgattatt tacacttagt gaagcatctt ctaatgtttt atgaacactc caattcattt
     5281 gtccccagga aggacactgc acattgttta ttcccatcca tctgcatcca cttcctaatg
     5341 gtatttttct gcctgcaaaa tttatatcgc caatgtttaa agtcgcataa acactttccg
     5401 gtattgcatt atcatatact aattgaacaa actctttttc caactccctg tctgttttat
     5461 tcattgcaat aaatgaaata ccaatgacct tgtcatcaga gtttttgaaa aagtaataat
     5521 tcatatttat tgtaattcct gattctgtct ttgatttaga aacataaaag tttgaaaatc
     5581 ctaaagtgct gtcagtagaa ataatttcct tttcctttat agccaaattc ttgaatttct
     5641 tagaaatact tttaggtgtc aagtcggctg gtactttttg agttgtgatt tcaatgccat
     5701 caacattaaa aaaatcaatg ccattgtttg aaattccgtt tagccgagca aacaaactgt
     5761 catggtcttg tccgaaagtg aaacatgaag atagaaaaaa tattactata aatatttgta
     5821 ttttttgcat cattttgtca ttgtgaaagt ataaacaggt cttccttttt catcagtagt
     5881 tttaatccaa ttgtctttat ttttaggatc attaaatgat agactgtctg tttgaaagat
     5941 aataaacttt ttaaaccaag ctgtgggaga agcatccttt ttaaagtttg ttcttcttgc
     6001 aacgacactg tcattcggaa ataccataaa tggtaaagtc atttcataag gtcccatttg
     6061 actttgtttt atgatgctgg ctttaaagtt aagtgtctta tctgtagtgt tttttacgta
     6121 aaaagtagtc aatggataat ttgtcataca gcttacagag aacaaaacta aaataattat
     6181 aaccaagtta cgcattcgtt taaagtgaat tagaaatcca aacgtttgat ataagcactg
     6241 ctgccaatta cttataacta ttgtttacat caaaagtaaa aagaaatgtg attagaatat
     6301 tgagagttga aagttgaaaa tgtaaaatgc aaaagtgaaa agtgatttga taattatgaa
     6361 tgtagaattc agagtttaga attgttgatc agtcacggtc agccacggct gaaaagcgtg
     6421 aaaaatgaca gtctgaaaat gctttaacaa gccctgatgt ttacctgtat ggtttttgtg
     6481 cgtgagcggt gccgtgcacc ggggaaacag caggaataga ttttgcagat gttactgcag
     6541 tttgagctcc aaaaaattat ttaaataatt acgattttaa aaatatcgtt cagaaaaaaa
     6601 gtacccggag tggtccgcca catccaccac aggcggaggc gaacccacat aaatgttttt
     6661 aaaataaaat tacttcgaaa aaagtacccg gagtggtccg ccactggcgg agaacccaca
     6721 taaatgtttt taaaataaaa ttacttagaa aaaagtaccc ggagtggtcc gccacatcca
     6781 ccgcaggcgg aggagaaccc acacaaatgt ttttaaaaaa attactttga aaaaaagtac
     6841 ccggagtggg aatcgaaccc acacggacgt ttaaggtcca caggatttta agtcctgcgt
     6901 gtctaccagt tccaccatcc ggataaaaag gaacttcaaa aaaaaagtta ccgaaaataa
     6961 acagctacag gtttaacaga ggccaaccgt tagctacatg tgtggcggta acttttgttg
     7021 agagcggaag acggggttcg aacccgcgac ctcgaccttg gcaaggtcgc gctctaccaa
     7081 ctgagctact tccgcataat tgcttgatat ttcaatactt aaattcccga cttcgttaga
     7141 agggattgca aaattataaa aaataaaata agaacaaatt ttttactttt tattttttga
     7201 aagcagcgtt tttatctcgt tgagtttaaa gagcgcctct acgggtgtga gggtgttgac
     7261 atcggttttg agtatttcgt ctcttatttg ttccaataaa gggtcgtcaa gctggaagaa
     7321 gcttagctgc atggcgtctt tgtcggcctg atgtccggca ttgcgcagca attctgtgtt
     7381 gctgtgtgct ttttcgagaa gggctagcat tttttcggcc ttgttgatga catgtggcgg
     7441 catacctgcc attcgtgcta cgtgaatacc aaaactatgt tcgctgccac ctggcactaa
     7501 tttgcgcagg aaaagtattt tattgcctgc ttcttttatt gccacattgt agtttttaat
     7561 cctgttgtag gagttggtca tttcattgag ctcgtgataa tgtgtggcaa acagtgtttt
     7621 ggcacgtgca tttttggatt cgtggatata gtcggcaatg gaccatgcaa tagaaatgcc
     7681 atcataggtg cttgtgccgc gaccaatttc gtctaacagc accaagctac gctgtgtaat
     7741 gttgttgagg atgctggcag tttcattcat ttcgaccata aaggttgatt cgccagaaga
     7801 gatgttgtcg cttgctccta ctctggtaaa gagtttgtca acccagccaa tttctgcact
     7861 cttggcaggg acaaaactgc ccgtttgcgc catgagtaca atgagtgccg tttgcctgag
     7921 caaggccgac ttaccggaca tgttggggcc ggtgatcatg ataatctgct gcgtttgctg
     7981 gtctaaaaaa atagagtttg gaatataggc ttcgcccgga ggcatttgtt tttcgagcac
     8041 aggatggcgg ccttctgtga tagaaagaac aaggctgtcg tttacaacag ggcgtgtgta
     8101 attattttcg atggcaacgt ttgcaaaaga caataggcag tcgagctgcg acaaaacaga
     8161 tgcattgacc tgcaagggct gaatgtagtt ttgtgtaaag ctgatgagct cctgaaaaat
     8221 ttcattttct atggttgaaa ttttttcttc agcacccaga attttgtctt cgtattcttt
     8281 gagttcggga gtgatgtagc gctcggcatt tacgagggtt tgttttctta tccagctttc
     8341 gggcaccttg cttttatgtg cattggtgac ttcaatgtaa tagccaaaca cattgttgaa
     8401 ggcaattttg agagaggaaa ttccggtttg ttcaacttca cgctgttgta ttttcagcag
     8461 ataatctttg cctgaaaatg ctacttgtct taagtcgtca agctgctgac tgaatccttt
     8521 tgcaatgaaa cctccctttg atgcaagtgg tggtgcatct tcaattagcg catcctgaat
     8581 tttttgaatg agtaggttac agggatttag ctgttctgac aaaacattaa acgtactgtt
     8641 cccggcagta gcaaggttgt cttttacagg ttgaatctga gataatgcac gtttgagttg
     8701 caccatttcg cgtggtgaaa ttctgcggag tgcaatttta gaaagcattc tttctatatc
     8761 gccaacatga cgcagagatg ttgctatctg ccctcttaat gtttcatcta aggtgaaagc
     8821 ggctacagca tcgtgcctct catgaatggt ttgaatgtct tttagaggta ggagtatcca
     8881 tcgtttgagt agccgcgacc ccatgggtgt gacagttctg tcaataacat caactaaggt
     8941 gcgtgcattt tcatgtggcg aggcaacaag ttcaagattg cggatcgtaa atttatcgag
     9001 ccagacatat tgttcttcgt caatgcgtgc aatgcctgtg atgtgcgaca gcgtttgctg
     9061 ctgcatttgt ccgaaataat gcaaggcaac accacatgcc acaatcattt cgtccatttc
     9121 gtgaataccg aaacctttaa gtgaattggt ctgaaagtgt ttgagtaaaa cttcttctgc
     9181 atattctttc tgaaaagccc attgatcgag tgtgtagata tagaagccat cgccaaagcg
     9241 tgcaataaaa tctttttgca tggatttctg caacacaact tcgctgggtt taaaattctg
     9301 aagcagcttt tctatatatg catctttacc acgtgcagta aaaaatgttc ctgtcgaaac
     9361 atctgcaaaa gctacacctg aaacatcttc tgtaagatga actactgcaa gaaaattatt
     9421 gtgtgtgttt tcaagaattt tttcattgta ggtaacaccg ggagaaacca attctgttac
     9481 gcctctttta acaatggtct ttgttgtctt tggatcttct aactgatcgc agatggcaac
     9541 acgctgcccg gcacgaacca atttaggcaa ataggtatcg agcgcatgat aggggaagcc
     9601 tgcaagctcc atttctgatg ctgcaccatt ggaacgtttg gtgaggacaa tacccagaat
     9661 ttttgatgtc tttacagcat cttcactaaa agtttcgtaa aaatcgccta cacgaaagag
     9721 taataatgca tcaggatact ttgcctttat ggcattgtat tgctgcatga gcggagtttc
     9781 gcttttattt ttttcttttg tcttcaattg taaaaaatga aaaagttaac cggcagcgaa
     9841 atgggtcgca cttcgacaga agggtttaag caaatacaaa aaattcctgt tgtcattatt
     9901 cttgaaaata taagaagtgc acttaataca ggctctgttt tcagaacctg tgatgccttt
     9961 ccggtagaaa aaattattct aacgggcatc acggcacaac caccacacaa agatatttta
    10021 aaaactgcat tgggcgctac agagtcagta aactggcaat attattctga tattaaagtc
    10081 tgtatttcta aacttaaagc aaatggttat aaaatttttg cagcagagca aacaagtcaa
    10141 agtatagcta tgcctgattt taaacctgaa aaagatgaaa agttagctgt tgtttttggt
    10201 aatgaagttg atggtgtgag caatgaaaca atagcaatgt gtgatggggc aatagaactg
    10261 ccgcaacatg gcaccaaaca ttccttgaac attgcagtgt gtgcaggcat aatgatatat
    10321 gacctgtttc agaaaatgaa agcataaaaa aagccggact aaagtccggc ctttccttta
    10381 aatattattc aggttatttt gctacttcaa aatcaaccct tctgttcatg cgcttgttgc
    10441 ctttggcaag ttgttctttc ttacccaatg atgtggcagt aagtcttgag gcatcaatac
    10501 cataaacttt tacaaggtga tttatagcag cttctgcacg tttcatacct aatttatcat
    10561 tgtattgttc agaggcatca acatcgcaat taccaacaac actcagtttc agcttaggat
    10621 tttttatcat cacacgtgca actgtattta agcggtcgtt gtaaacagat ttgatagctg
    10681 actggtcaac atcaaagaat attgaaggta accaacctgt agcgctgtta gaagtgatac
    10741 catcatttcc ataagatccg gcaggtgcaa tagtcatacc ctggaagttt actaagtttc
    10801 ctttaggagt gtttggttcc atatcacgac tgtcaggaac accatcgcca tcagtatcaa
    10861 cctctaaacc actggcatca actttagctc ctttatttga ataagggtca gagtccacat
    10921 tatcaggaac accatcacca tcgctatcta aggcagtacc atcaccataa actttaacgc
    10981 cttcaggagt tgagttgtct ttgtcgaaca aatcagaaac accgtcttta tctttatcac
    11041 cagacattac atctattctg tctttcatat cagaaagatc attataaact acttccattg
    11101 ggttaaccca ttccatcatt gcttgttttt tgccgaatgt atagttaaca cctaacagga
    11161 aaaacgacca gttgtcactc tctgttttag agtcatatac ctgatctact ttatcgttaa
    11221 gagttttgtt aaatgtggca agtaaaccaa catcaaaacg cttgatctga tatttaaatc
    11281 ctaaacttaa aggtaaaaat ccttcagcta cagagccttt ctttacataa acagttccat
    11341 tgtcaagatc agtaactttt ggagcatagc taaatatacc caaaccaatt tcaccataaa
    11401 agtgcaaacg cttgttacgt tgtaaaaagc tgatgttacc tatggtataa tttaagttaa
    11461 gacttgcctg atatataggt gacttaaact taatgttcct ggtaatttgc gggctggtaa
    11521 cagcatttgg aacacggaaa gttgtcttgt tcttttttcc gctcaagttt gtgtaattac
    11581 cacgaacgtt tacctgcaca gagtggctta tctgatagcc tactttaagt ccgaatacag
    11641 ggtcttggat gttattcagg atactgttat tgtttccggt tttttcgttg aggtcgccct
    11701 gaaaccagtt taatcctaca gttagtcctg ctgaccattt gtcaaaatcc ttgttcttag
    11761 gaaggccgta tctggccgat tcctgagcga acgcaaattg cgtagcagag attaaaaaag
    11821 ctgaaacgag taaggttttt actttcatcg tatagagtgg tttaatattt tacaaaattt
    11881 tgtggtgcaa gtataacaca atggttttaa ataataaaat taaatatgaa gtaaataaac
    11941 agatttattg acagtgcttg taaatcagaa cctaaaacct gtcttttcta tagtttctac
    12001 aattttttga aaatcgcttc tttttcgcac aaaatcaagt ccttccgtat tgattattag
    12061 gatattggaa gctgtgttgg actttttcat aaaacgacta tatgcattat tgattttgag
    12121 tagataatcg tccttcatat tctgctcgta ctcacgccca cgttttttaa tatttttttg
    12181 gagcgaaggt atttcctttt gcaaatacac aattaattgc ggcagaggca atcctacatt
    12241 gagcatctga aaaaactgtt caaaaagccc atattcattt tttttaagat tcgacttggc
    12301 aaatagcagt gttttttgaa acatataatc agacacaata ggaaaaggca gtgtgttttt
    12361 gttgaaaaat gttttcaatt gcttataccg cgcagccata aatgacattt caagcgggaa
    12421 ggcaaatttt tcaggactat cataaaactg ctgtaaaaaa gtattttctg cgaactcttc
    12481 taatataaga tgataattaa actcttcggc aatcatcttg gctaatgaag ttttgccggc
    12541 cccaatgttt ccttcaatag ttaaaaatct atttctgtac atataaagga tatttagaga
    12601 ctttcaaatc atctttgcat tcaactagca attcagaaat acttttttta aaaacaggat
    12661 gaataaaatc agaaataagt tctgcaagag gctcgagcac aaaacgcctt tcatgcaaat
    12721 gtttatgtgg aactgtcaat tccggtgtac agataatttc attattgaaa aaaagtatgt
    12781 caagatctat gctgcgtggt tcccactgat gcttgcgtgt tcgtccctga gatttttcaa
    12841 aggtcaataa aacatcaagc aattcgtgtg cagtaaatgc attataaatg ctgaatgcct
    12901 gattcagaaa aggcggctgg ttctgattac cccagggtgc tgattcatac actgatgatg
    12961 atgccatttt ttttgttgac aacctttgaa gaaacaatgc tgcctcagtc agattatgta
    13021 gtctgttacc taaattactg cctgcaagta taattgcagt attctgatgc aaagccattt
    13081 tgatttttat agctttgcaa agctaaattt tcaaactcac acaagtccat ttaaaatgaa
    13141 acaatttctg aaatatgttt tggccacact cacaggcctg attcttttct gtcttttaac
    13201 attcttttta tttgctgtta tgcttggcag tgcctttaaa aaagaagttg tagaagttaa
    13261 aagcaactct gtattaaaaa tagaattcaa aatgcccatt gcagaacgta gcgacaacaa
    13321 cccatttcgc aatctgaatt taagcacgtt gaaggcaagt aaaaaaccag gactaaatga
    13381 tattgttgcc aatatagaaa aagctgaaaa ggatgataac attaaaggta ttctccttaa
    13441 tctgaattat tttgaagccg gctatgctac tacaacagaa atcagaaatg cattagtgaa
    13501 atttaaaaaa tcaggtaagt ttatttttgc ctatgctgaa tattattcaa ccaaagctta
    13561 ttatctggca agcgttgccg ataaaattta tgtacatcct gaaggtgctg ttgattttaa
    13621 aggccttttt acacagatga tgtttatgaa aggtgcattg gataaacttg aaattgaacc
    13681 tgaaattatc agacatggaa aatttaaaag tgctattgag ccatttattt tggataaaat
    13741 gagtccggaa aacaaagagc aggttgccac ttttatcaac tctatatggg aaactataag
    13801 tagtgaaatt gcagaatcgc gcaaaataag tcgcgaagaa ttagaacaaa ttgctgacag
    13861 tttatctgca gaaaatgcaa aagatgcact taggttaaag ttagttgatc aattatgtta
    13921 taaagatgag ttggaaaaaa ttcttgcaga aaaatcaggt gtgaatgatg atgatatcaa
    13981 ctacatcagc ctgaacaaat acaatgaagc tgttgttaag tcggataaaa aattcaccac
    14041 tgaccgcatt gccgtagttt atgcagtggg tgacattgta agcggtagtg gcgaagccga
    14101 ccaagtaggt tctgatagaa tagcagaagc aataaaaaaa gctcgattag atgataaagt
    14161 aaaggcaatt gttttaagag ttaattctcc cggaggcagt gcattggcct ctgatgtgat
    14221 atggcgagaa actaaattag ctaaggagaa aaaaccattt attgtaagta tgggtaacct
    14281 tgcggcatca ggtggctact acatcagttg tttggccgat accattgttg cgcaacctac
    14341 caccattact ggctctattg gtgtttttgg attattattt aatgcacaaa aactgctcaa
    14401 caacaaatta ggattaacat ttgatactta taaaaccgga ccttatgccg accttggtat
    14461 gccaacaaga ccacttgagc ctgctgagag aaacattctt cagcgcagtg ttgaagatgt
    14521 ttacagcact tttataggac gtgttgccga aggtagaaaa atggaaacgg ttatggtgga
    14581 tagtatagga caaggacgtg tgtggagtgc taccgatgca aagagaattg gtttggttga
    14641 tatttatggc ggattgaatg atgctgttac tcttgcagca aaaaaagccg gattggataa
    14701 ctacagaata aaagaacttc ctgaacaaaa agatccaata caggaaatac ttgaagatat
    14761 gaaaggtgaa tctgaaagta tgatgatgcc cgactacatg aaagaaagca tgaagctgat
    14821 gaaatatctc aagcaaattt cagaacaaga caaaattcag gcaagaatgc catttggcat
    14881 tgctgttgaa taaaaagcag caatcatttt attgtagtca aaaaaaacat tgactgctgt
    14941 aaaactactt acttgtgagc gattttattt tatcaaataa atcttcatgg gtaaacggtt
    15001 tgctcacata ggcattcatt cctactgcaa aacatttgtc aatttcttct gatattacat
    15061 tagctgtcat tgccattata ggcacaccat ttaacggtga cggcatttgc gtacgaataa
    15121 actgagttgt ttcatagcca tccatttcag gcatgtgaat gtcgagaatt actaaatcat
    15181 aattacctga cgtgagtttt tctatagctt gcacaccatt atcaacaata tccaatgtaa
    15241 gatcaggaat aaccatacgt aaagtatctt ctgctaccat ctgattaaac tgattgtctt
    15301 cagcaagcag aatccttaat cctttacgga tttctacact tgaacttacc ttgtcttttg
    15361 tttcagttgc caattcattt gttgctacca atggcaattt cataataaaa gaagaacctt
    15421 ttccttgcgt actttcaaca actaattcac catgcatcaa ctctatcagt tgtttgctga
    15481 tagcaagccc aaggccggta ccaccaaatt ttcttgttgt atcagagctt tcctgtgtaa
    15541 aactttcaaa aatgtgctgt attttatctt gagcaatacc aataccggta tcaactacgc
    15601 taatgctgac accgttagga acatcagcta ttgaagcaac tctaattgtt acagatcctt
    15661 tttcggtaaa cttaatagca ttacctgtaa gattaatcaa cacctgtgtt atccgcacag
    15721 ggtcaccaat ataattatca gaaagtgttg aacaataatc cacattaaag gcaagacctt
    15781 tttcttcagc tttcacttta agcgactcat aaacatggtt caccttttca tgtaggttaa
    15841 acggaatttc ctcaatggta aacttgcccg attcaatttt tgaaatatca agaatatcgt
    15901 tgatgatgac caaaagattg tcggcacttg tttttatgat gccaatatac tttgactgtt
    15961 tatcatcaag gtttgattta tgtaacagat ttaccgttcc tataattgca ttcattggcg
    16021 tcctgatttc atgactcatg tttgcaagaa actgctgctt gagtcttttt gattcttcgg
    16081 ccttgtcttt ttccagtact gcaatttcct tctcaataat agtttgttgt aatgcctcct
    16141 gtgccttttg acctttttta attatttcaa tttcgctcaa acctttacgt aaagtagtgt
    16201 caaggtcggt catgtctata ggtttggtaa taaaatcaaa tgctccactg ttcatcgctt
    16261 tacggatatt gtccatatcg ccataagctg aaatcacaac tgctttaggt tgaatcttgt
    16321 tttctttaat ttgattcagt aaggtcaatc catccattac aggcatgttg atgtctgtaa
    16381 aaatcaaatc aatatcaaca tcatctttca gcttttgtaa tgcttcttca ccattgtgtg
    16441 caaaatcaaa ttgcaattca ttgttcctaa tctgcgaacg aaaccgttgc ttgactaaaa
    16501 gttcaagatc aggctcatca tcaactacca atattttttt tgccataact aaattgttat
    16561 cactcttgcc atttttcttt taagatcttc aaagtcaaca ggcttagtta caaaatcatc
    16621 tacacctttc gccttagctt cattataatt cttatcatca ccataagctg acaccataat
    16681 aattcgcatt gcaggaaatt cagcctttac tttttcaacc aactgaagtc cgttcatgcc
    16741 cggcatattt atatccgaca ataccaataa aacatccata gggttaatgt tgtgaagata
    16801 gtttaaagcg tcttcaccac taaatgcaaa gtgaagatca atatgtttct ctttgatttc
    16861 acgtctgaat tttagcctga acaacagttc aacatcacgt tcatcatcta ctatcagaat
    16921 ttttgtttgc atgatatttt tattatagtt ggggtaattc aataacaaac tcagacccta
    16981 tacctttctg cacattcaca cttagcatac cataatgacc tttggtgatg atatcataac
    17041 ttaatgataa acccaatcca gttccttcac cggtaggctt agttgtaaag aatggttgga
    17101 agattttatc aacgatatca tcaggaattc caccaccatt atcactgata ctaatataaa
    17161 ctttgtttgc agctttccat gttctaagtc gaacttcagg atgaaatgct ttatcagatt
    17221 ttttacgtag ttgatttaca gcataaaatg cattgttaaa aatattgagt aacacgcgac
    17281 tgatatcctg ctgaactatg ctgagctttg gcaatgaggc atccaactca atcatattct
    17341 tacacccaaa attactatcg tttgctcgaa ggcttgtata ggcaaggttc attacatcct
    17401 ctgcaagttt gttgatgtct gttaaaactt tctctgctgt ttgcgaacgg ctgtgcatga
    17461 gcatactctt cactatacgc tctgcacgtt taccatgctc attaattttt tcgagattgg
    17521 tttttatatc atgagaaata gacttccatt cttctttgtc atcagtagca ttcaactcat
    17581 caataagatc ttgagataac tccgaaaaat tgttaacgaa gttaagtgga ttttgaattt
    17641 catgagcaat acctgcagta agttgaccta aagaagccat tttctctgca tgtatcagtt
    17701 gcgattgtgt gtctttaagt tcctttaatg cgcgctcaat ttctaatgac ttctgttctg
    17761 atttttcttt ttcttctcgc aattctgctg tgcgctcttg tactttctgt tccaaaattt
    17821 catattgttt catcagcgtt cgtgtacgcc aacgaatgta agttatggtt aaacctacga
    17881 aagtaagacc ggataaaata tagaaccacc aggttttcca aaacggtggt ttgatgatga
    17941 tgataatata tgttccatcc tgattccaga ttccatcgtt gttacatgct ttaactctga
    18001 acttatacgt gccggggtca agatctgtat attggcgatc gcgtttactg ccacaatatg
    18061 tccacgtctt ttcaagtcct tcaagcatgt aggcatattg atttttttcc ggtagaatgt
    18121 aatttaaagc tgcatattca aatgaaaaaa agttttgatt gtaagttaac tctatcatat
    18181 ttttggacat gataatcgtg tccatgaaat aaggtttttc aaaaagataa aattttgtga
    18241 taactacagg aggtatgtaa ggattatcgc gaatagctga tggcaagaaa cgattaattc
    18301 cactgatgcc cccaaagtaa attttgccat ctgcagtttg tgcaaatgca cccggattga
    18361 agcgattaaa aacaccatct tgctcatcat aaattctcat ggtgagaata gaatctgaag
    18421 gagccagttt cattttgcag atttgatttc ctgtagtaaa ccagatattt ttttctgcat
    18481 ctgaaacaat attcgtcaca ttattattgg gaacaccatt ggtttcagtc aatgcagtaa
    18541 aactttttgt ggcataatta aaaatattta ttccaccact gtatgtgcca acaagcaatt
    18601 gctgatttga aaaactgtat attgagttga cataattact gctaattgtg gtattgcttc
    18661 cgggctttga ttcaaatgct gttatggtat ttgcagaaat gttcaacaaa cataatccac
    18721 caccataggt gccaataaac aaatttccat tgccatcata ttttaaagcc gttattatat
    18781 tattcgttaa acctttgcca tcttctttac ggatacttga aatcttatcc gtttgtctat
    18841 taagtacgca aagcccacca ccgtaggtgc caatataaat atttccattt tcatcttcgg
    18901 caagcgatct gatattacta ttggtcaaac cgtctgctat ccgatagcta cgcacaactc
    18961 ctgactgtgg gttgatggac gttaaaccat tataagttcc aacccaaaca ataccatttc
    19021 tatctttgat taaacagaac acactgttgt ctgcaagtcc gtttgcagca ttatatactt
    19081 gtttgattgt gcctgttgat tctttaacaa ccacacctgt tcccaaaaca cccatccaca
    19141 aattattccc ctcaggcaga aaacaaaaaa cattcttatt gggagtcgca ttactcaatg
    19201 gatcatccgg cagtggcaac tctgttttaa aacgcatcag gtcttccttg taataatcaa
    19261 gaccattgtc tgttccaatc cagattatgc ctgccttatc acgatatatt gtccaaatgc
    19321 tattgctgct gatagaaaat ggatctgtct tactatgtgt aaatgaagaa taagttgaag
    19381 catcgctgtt aaaaattatc aaacctcctc ctatagttcc ggcaataata cttccatcaa
    19441 gatccttatt gattccgaaa acggagttgt ttgcatactt gcccagattg gtgtaatgag
    19501 taaaagaacg attgtctgtt cgcatagcac tcatgccact gtttagagtt ccgatccaaa
    19561 ttttattttg aaaatcggca taaagacagg taatgctgtt ttctaaagga gaatttggat
    19621 ttgccgggtc atgatcaatg atagtaaaga taccgggttg atttctatct gcaattgcaa
    19681 gaccattatc tgtacctaaa tacaatacac cattcttatc ttttacaata gctgtaatat
    19741 tattactgca tattgaattt ggatttgact tatcggattt aaaattctgc cattttccgg
    19801 ttgaaggatt aaaaaggtta atgccgccac cggcagttcc aatccaaaga acaccagagg
    19861 catcttcttc catacaggtt atctgattgc tgctgattga atttcctttg gtgtttgttc
    19921 tgaaacttcc gaaatgttca gtttcaatat cgaaaaagct tagtccatca tctgtaccta
    19981 tccacaactt tcctttactg tcttcaaaca aacaacgaat gccattgctc gaaacagaag
    20041 tttcatcaga tgggttgtgc ttataaacat caattttata gccattgtat ctgttcagtc
    20101 cgtcttcagt accaatccag ataaatccaa ttttatcctg aatgatacaa gtaattctat
    20161 tgtgcgaaag tccgtttaca gtgttgagat gcctgaacac atcactcttt tgtgcaaaag
    20221 cattgatggc tattagaaga aataaccaaa aaaggcgggt gactttttga attgcttttg
    20281 aaggcatggg ttcaaaagta agatttattg tttaattccc gtgccatgac aaactttaca
    20341 tgttgcccat gctgcaggtt tcactacttc agacactgtg taggtaacaa aatttcgggg
    20401 acctaaacca ttactgatat ctttaacaac tgagcggttt cgcttaacct ccgcttgaag
    20461 aatttttccc tttccattac aggcagggca tggaatttta gcaacagttg gctcagaaac
    20521 tttaggctct tcttttttat cctcaggttt ctttgcctcg gttttatttt cttccggcac
    20581 tggtttcaca ttttccttgt caatgacttt cgattctcct actgccctac cattttcata
    20641 cattgtaaca gtctcttcac ccgactcatc aatataagtt ccttcaccat taaaaacgtt
    20701 atttagaaaa gttcctttat aacgatctcc tgtggcataa gcataaacac cacctccact
    20761 gtataatcca tctctgaatt ctcctgtata gacatcacca ttagaataat aacatgttcc
    20821 ctgaccatta aacaaatcat ttctgaactg acctatatat gtacttccat cagcaaacgt
    20881 gaaaacaccc aagccattgt aaacgtcatt cacaaaatcg ccactataac gactactgtc
    20941 attataataa taagtaccct tacctgattt ttttgaattt acaaaatcac cttcatacca
    21001 atcgccattt gcatatctcc aggtgccttt accctgtacc atgtcatcta caaactgacc
    21061 ggtgtatgta cttccatcgt tgaaagtaaa tgttccactg ccatgtttgc ttccttcaaa
    21121 aaaatcacca atatattgat tgccgctgtt gtaaatataa ataccgcgac catgaaactg
    21181 atcgtttaca aaatcacctg tataactatt tccatttctg taattgtagt tacctttacc
    21241 atttaacctg ccttgtgtaa aatctccttc atagcgattt ccgtttctgt aattcataat
    21301 gcctttacca tctggactac catttttaaa agcaccttca taacgattac cattcttata
    21361 taagtaaacg cctatgccat ttacgcagtc gccactttga caacctacaa tatttgttgc
    21421 acggtttaat gtgtctgact gcgaaaaaac agtcaatggc attaacacga aaaataaatt
    21481 atacaacaga tttcttaaca tctccattac tttgttttag atcaggtggt gcatttagcg
    21541 gcaaagaaat tgtaaaaacc gttccaacac cttgttctga ctcaaatttt aacttacccc
    21601 catgttgttt ttctattatg tcgaaactta ctgataaccc caatcccgta cccttccctg
    21661 ttggtttgga tgtaaagaaa ggattataaa ttttatcttt gatggtatcc ggtatcccag
    21721 ttccattatc agtaattgta atttccagaa actggccggc aacatgtgtt gtaacactaa
    21781 cctgtggttt atacttaact tcttctttat tagcatttga ttttacagca cgctcatgta
    21841 cagcataaaa ggcattattg ataagattta taattactct acgcaactcc tggggtaaaa
    21901 ctttaactgg aggtgctgtt gcatcaaaat ctttttcaat atgacaataa aaatctgcct
    21961 ctgttgattt cttgccatga aaggctaatg ctaatgattc gtcaacaaga aggttaatat
    22021 ctgacaattc tttttctgct gttttgtttc tactatgcaa caacatacct tttacaatag
    22081 aagaaaccct tcctccatga tgattaattc gtttgagatt cgacttcaac tcatcaagta
    22141 aagtttcatc tttacttttc agataatcat caatcatatc catacttaaa gtagaaaagt
    22201 tatttacaaa attcaacggg ttttgaattt catgtgcaat acctgcagta agctgaccta
    22261 aagaagccat cttctcagac tgcaccaatt gttcctgtgt ttgttttagc tgctcatgtg
    22321 cctgccgcaa ttcctgatga gcctgcctga gttgctctaa tgactttctt ttaagcctat
    22381 agcggttaat caaaaatact attcccaaga ctatcaatcc ggaaatacct aaaagaatat
    22441 ttctaaattc cttttgcctg ctgatttgca gttctttttt agcaagttcg gcttcttgct
    22501 ttaatttttc tcctgcaatt ttatccagct caagttcttt cagtttcttt tgtgcttcgg
    22561 caagtgttaa ttcttctaaa gccttctcct tattctgacg ttcgatgtca agcgtagtta
    22621 actgttgact aagttcatat tcctgccgcg aaaccatatt gttttcgttg ttggccgact
    22681 gacttaataa aaacagaatt ttttcttttt ctgcctgcaa tgctgctgtt tccttaacaa
    22741 cagtttcatt agcttttaat ttcctatata actcatgata gcgatctgcc ttttcaaaat
    22801 cgttattaaa actgctgatg gttacaattt taagataggt gtcttttagc aacttctgca
    22861 aaaggacatt atctctaaaa tccaaacttt gtctgaaata ggatacagct tttaaataat
    22921 cgtcacgctt taaattacat aaacctaaat ttcgggtagc caatgcctga aatgttttat
    22981 tgttcaactt cgtggcatac ttcaattgtt gtaaataatt agcagatgaa ttattgaaat
    23041 tttttgcaac aagattaata tcacccattc tttcgtacag ataatattgt tgaagtaaaa
    23101 aattattggc tgaaaaatat tgtaatgcat tgccgagtaa tggctttgct gaaactgtat
    23161 ctgcatgttg aagaaaaatt aaacctgcca ttgcctgaac ataggcccta atttgtgttg
    23221 agttggctaa atctttttcg agtaattctt tataaataga aaatgcctgt tcatgcctac
    23281 ctgcaacatc aagtgcaaat gcatattgcg ccatagttgg cgcagcttcc ttactcagtg
    23341 gcttatgatt tactgctatt tgtaaaaatt ttaatgcctg aagaaaatca ccattgagta
    23401 gattgtagta tccggcatta ttggcatttt ggaaaatttc ctgctgacgg ttgctcttga
    23461 ttgcatcgcc taacattctt tccgcagact ttaatgcaat agggtcagca gttatagtta
    23521 taaaaaaagg tcttgaaaaa acaggtaaat catttctgtc aaaaactgaa taaagatgct
    23581 ttgcatcacc cctaacaaat gtgccaatga gacaaaagat aaaaattaaa acttgcttct
    23641 tcatcaccga gacgaatgcc aaatgtattc aatttttatc aattgacgat gtaactttca
    23701 atcttattgt ctaagcttaa gtccaaaccg gaatgaataa aacgggcttg ccttgccgtc
    23761 atagctgcaa acaaaatcag ctcgtattac tccgagcttt tctaatccta cagtaagttc
    23821 ataatagctt ttcatttcac taactttcag gaagtggaag cctacaactt cattgagttt
    23881 caactttctg attaaaggaa ttttattggt aataaaacga ttcagattgt attctgcatg
    23941 aattgcaaaa gcacttttca tattcgaata tttgtaataa tctaaataag caaaatcttc
    24001 agccttaaaa tctgagacga tagttttgtt acccgcaaaa tgaaaaaagt cgggaaattc
    24061 aacatctttt cgattaacaa aacgataata cctgcaattg taaactacat aacctaacat
    24121 tccaaaatct atctgatgat tgaagcctgc atcaaatgaa agataatcag tcttactgtt
    24181 tattacattg ccaaaagctt tattgatgtt cacaaaaaat accggccacc ctgattcttc
    24241 tctcactttt ccatcaggcc ttgtaatgaa cttcgtaaat ggtgagtagg ttaatgatag
    24301 atgtgtgtta aagtaatgat gaggtcgaaa attttttata tccttatcag gattaatggg
    24361 attattggaa gtatattctc tgtttttaaa attcttccaa gtataatccg tggtgttttt
    24421 caatgtattt cttcggccat attcaatttc tgcagttgca ttaaacccat taaaccattc
    24481 tctaccatat gctattttaa aaaattgctt ttcgtaaatt tttaaataat tacgttcggc
    24541 ccatagcgta taaagcatgt tgactaaagg tttaacaggg cttgtgctgt ttaattcatt
    24601 aacagcagta ccgccttcaa cagaaattgt attatcacta aagcggttaa acaaatattt
    24661 aaaaccagca tttgcattcc aatgtgtatt gctaaaacca taacgtgtat tggtaaaaac
    24721 agtcaggcgt tttcggtttg gcaaacgttt aacaaactct gtgttcaagg caacattcca
    24781 gccttccact gtattaaatg aaaatgtatt ggcaattggc tttatagtat aagtcatttc
    24841 ccggtaactg tgtctgtagg aatatccggc aagcaaaaaa ttaaagaacg aaaacttatt
    24901 atttattcta tccatactat ctttatactg tttactctct cgaatgatag ccaaactgtc
    24961 tttcttaaaa taatctgaag tttcatctat cgtaagtggt ataggtctta cagtatccca
    25021 atatgcagaa tccttcttat tggcatcagg actgattttt aaaatttctg ctgaaaaggt
    25081 tttttttgga aaaccgggat ttattttaaa gtcagaaaag tagcctatat aatttccgcc
    25141 tccctggaaa ccaagaatcc tgaaactgaa atcaaataaa tttgtcacag gcatccaaac
    25201 attattttct acagggacaa aagactgcct tattctgagt gtatctaaaa actcaatacc
    25261 tgcatcgcgt gtaaggaaaa gatcaacaaa ctgaaatcgc cataaatcat ctgcaatata
    25321 aataacacct ctgaacaccg gatcgttctg tcttctgggg attacctcaa ttttatgaat
    25381 aagtctgtcg ttctctacaa ctgaaccaat taatttataa cgataataaa acaaagcatc
    25441 gcccgcaact ggtgagacaa atcctttgga agaaatgcct tctgacataa ccctgttttc
    25501 ataaaaatta aattgcatat cagatgcttt attaaagctg aatgccctat tattaccact
    25561 tatgcgcgaa gaaatcatct cttcacgaat cttattcggt ttttcgaaac tgtattttga
    25621 aaccgattca cttaaataaa atatgcctgt tgttgagtca accactccac caaaatctac
    25681 aaccttacca agaaattttt caggatactt atttaatttc tgtacacctt tgatataaac
    25741 attgcacgaa tatgcatcaa cttcgttcag ataacttttc cttttagcaa ttgtttttct
    25801 gatatattca taggctgggt cttctttatt gtttgtaaca gcaacctcct ttaacgaata
    25861 aagctcatct gtaagttcta tctctatttg ttgtttacct gcaccaactt caatctgttt
    25921 gctgaattgc ttatagccca ggttctgaca ataaagagtg tacttaccgg gaactaattc
    25981 aatttcaaaa ataccgtcac tacttgttgt cgtcccaaca aaaccgggtt tcacatatac
    26041 attggcataa gctacaggtt tatgattttt atccttcact actcctgata caataccctg
    26101 agaaaacact gtcgtactaa aaatactgcc taacagaacc aggtaaaaaa atttacgcat
    26161 cacaacaatt aattcttaat ttcaggatgc taatgtaaaa ctttaaattt gggtttcaga
    26221 cagcttccgg aactttgctt ctctgaatgc ggaagaatga cttaagcatg tgtttcataa
    26281 tagccataaa acgaattcta cggaacggaa aacgccagtc cgctattaaa gcctttgaaa
    26341 gaaaataaat accatcttta ttttttcctt ccagcatgag gtgcaatgct ataaaagaaa
    26401 aacattcaca ttgaatctga ttaaatcgtt taccaacata ttgcataaaa tgttgatcag
    26461 cttgaagatg atctaagaga aacatgtttc tgttaatcat ctttataata tcgtgcgaag
    26521 ccatactacg ttgttcatgg tcaattacta cactggtaat ttccggcaca taatgtattt
    26581 tgaaccgtga ggacaaggct agccacaaag cataatcttc agtgcctgaa agaagacggc
    26641 tttcacaaaa tggaaacttt tcagcaatat cttttctgat tactacagaa ttacaactta
    26701 aaatattacc tttttttatg agcatttcac cgatattatc agaatctgct ttcaggacat
    26761 ctaaatcctg tccatcaagt gtttgataaa cgtaattaag gtgaaaaatt tcagggttat
    26821 caaacttcgc aatataattc agtgcagtag aaatatgatt attataagct aaatcatctg
    26881 aatcaaaaaa attaataaac ctacctttag caattgatgc tccatagtta cgtgcaaatc
    26941 cacgctcgct gtttacaatt ctatggtagg ttacctttgg atgtttatag gttgaaaaat
    27001 attcctctgt cttatcggta ctaccatcat caacaatcaa aatttcaaaa tcatctgact
    27061 gttgattcag caatgactca atcgttttta caacaaaatt caacctatta taagttggga
    27121 ctactacaga aatggaaacc atgagaaaaa gtattgcaaa actacagtac aataactgaa
    27181 aacaaacatt tcttaaaaaa aaattatccg ctaaatgtta accggctaaa aattgcagca
    27241 aacagatttt acctattttt gaaaccgtta agatatgaac catagaccaa cagaatttac
    27301 agagcgaaaa aaaggcgcta ttgaagtcat ttgcggcagc atgtttagtg gtaagacaga
    27361 ggagttgatt cgcagactaa aacgagcgcg cattgccaag cttaaggttg aaatatttaa
    27421 accttctgtt gatcaacgct atcatgaaag caacattgtt tcgcacgatt caaactatat
    27481 tccatcatcc cctgtccaat catcctctca aattctttta ctcacaaacg atgttgatgt
    27541 tgtaggcatt gacgaagcgc aattttttga tttggaactg cctaacgttt gccttaaact
    27601 ggcacatcag ggaatcaggg taattattgc aggtttagat atggacttta aaggcgaacc
    27661 tttcggacct attccaagac ttatggcaat agcagacttt gttacaaagg ttcatgccat
    27721 ctgtatggat tgtggcgacc ttgccttatt ttcttatcgt accgtacccg atgaatcact
    27781 cattgttctg ggtgaaaaag aaagctacat tcctttgtgc agaacttgtt atcataaaag
    27841 gtaattgtta ccaatttgat gcactatcaa agcttaattt tgtaggctat tcaatttaaa
    27901 aatatgtaca ccattacaga ggcagcgtca atattaaaag gaacgctggt tttgaatgta
    27961 aacgataatc agactttccg ttatctgcta acagacagcc gccaaagcgg tgtggccggt
    28021 gagtcacttt tttttgcaat taaaggcgac cgccatgatg gtcatttatt tattaatgat
    28081 cttatcagaa aaggagtaaa aaactttgtt gttagcgata cacacacttc acaaaacaaa
    28141 gaagctaatt ttattgtggt ggaaaacaca cttgacgcac tacaacaact tgcctcgttt
    28201 catcggaata aatttaatct gaaaacaata ggaataactg gaagcaatgg aaaaacaatt
    28261 atcaaggaat ggctttatca attattgcaa ccagatttta gtattgtccg cagcccaaaa
    28321 agttttaatt ctcaaacggg cgtaccatta tctgtttggc aaataaacaa tgagcataac
    28381 ctcggaattt ttgaagcagg catttctata cctggtgaaa tgcaaaagtt ggaaaaaatt
    28441 attcagcctg aaattggaat tttcagtaat gtcggtgaag cacacagcga aaacttttct
    28501 tcacttgatg ttaaaattca ggaaaaatta ttgttatttc aacattgcaa aaccctgata
    28561 tactgcaaag actattcagg aattcacaat gctgtaatac aatctggata tcttgaaaac
    28621 ggactaaaat gttttagctg gtcgcgaaga ataaaatccg atttacagat tggcagaata
    28681 gaaaagcaaa ataacagcag cacaattcag ggtatttttc aacagaagtt tataaggatt
    28741 gaaatacctt ttactgatga tgcttctatt gaaaatgcta ttcattgctg ggcaatgttg
    28801 ctctttttgg aaattgaaca agaaacgatt gctgaaagga tgatgttact gagtcctgtt
    28861 gccatgcgat tagagatgaa acagggtatc aataattgtt caataattaa tgacagctat
    28921 aattctgatt taggttcgtt gcaggttgcc ttagattttt taaatcgctt tcagcagcaa
    28981 aacaatacag gagcaattaa ttcaggctta cgcaaaaccg ttatcctttc agacattctt
    29041 cagagtgggc gaaatgaaga tcaactttat ggtgaagtgg cggaaatgat tcactctaaa
    29101 aaagttgaca gacttattgg aattggccct gcaatatcac gtcatacaga tttatttaaa
    29161 acagaaaaag tttttttttc tacaacagaa aaattcattg aacagtattc tccaggctca
    29221 tttattaatg aagttatatt attaaaaggc gcaagggttt atggatttga acagatttca
    29281 agattgttac agcaaaaagg tcatgaaaca gttttggaaa taaatctgac ttcaatgatt
    29341 cataattata acttctacaa agcaaagctc aaaccggaaa caaaaataat gtgtatggtt
    29401 aaagcttttg catacggtag tggcagttac gaagttgcca atacactgca atttcacaga
    29461 gtggactatc tggctgttgc ctatgctgat gaaggtattg aactcagaaa aaatggtatt
    29521 actgttccaa ttatggttat gaatccgcag gaacagtctt tcgataaaat gattgaatat
    29581 catttggagc ctgagatata tagtttcaga atcatgagca agtttagtga atctgttaaa
    29641 cgaatccgga cttacaatca aagcccattt cctattcatc taaaaatgga tacaggcatg
    29701 cacaggcttg gctttgaaga agacgatttg aatgaactcg ttgtacgttt aaaaaacagt
    29761 aaacacctta aggtagaatc tatttttaca catctggcag gaactgatga agcagcactc
    29821 gactttttta cacaagaaca gtttcagcga ttcaaagaca tgtctgatgc tataaaaaaa
    29881 caccttcagc acaatgtgtt ggagcatgtt ctgaattcat caggcatcag tcgctttcct
    29941 gaaatgcaac tgagcatggt gcgtcttggc attggccttc atggcatagg cacaccttct
    30001 gagcaacgtc aattattaaa tgttgcttca ttgaagtcaa ccatttcaca aataagaagt
    30061 gtaaaaaaag gagaaagtgt tggttacaac agaaaggcaa tattggatag cgataaaata
    30121 atcgcaacca taggcattgg ttatgccgat ggcataaacc ggaaaatggg aaatggaaag
    30181 gttagctttt tagtgaatgg caaacttgcc ccaacagttg gcaacatctg catggacatg
    30241 tgtatgcttg acattacaaa ctgtcaggca cgcgaaggtg atgaagtaat tatttttggc
    30301 aacggtttgc ccgttgccga acttgctgca aagacagaaa ccattcctta cgaaatttta
    30361 tcagtgattt cgcaacgtgt caaacgaata tattttcagg aataattaac ctttcgataa
    30421 ttctattaca gtaatttccg gaaggatacc taccctaccc ggataaccaa gaaaaccaag
    30481 tccgcgattt acatacaaat gctgaccatt tacatcatca tacaaatcag cccactcttt
    30541 ataaacgtac tgaacagggc tccatttaat ttttaatgca ggaatctcaa caccaaactg
    30601 aaatccatga gtatgccctg ctaaagttaa atcaatctgt ggataatttt cagttacttc
    30661 tccatgccaa tgcgaaggat cgtgcgtaag taaaatatta acgggagcaa attccattcc
    30721 ctgaactgct tttggcaggc taccatactt tggaaaacgc atgtgtgtac tccaattctg
    30781 tactccaatt aatcccaact tatcattgcc ttcagaaata aatgcatgtt catcaagcat
    30841 cagtttccaa cccatttcct tatggatatt tttcagttgc tccaaatttt taactttagc
    30901 ttctctgctt ggccattgca catagtcgcc ataatcgtga ttacctaaaa tagaaaaaac
    30961 accatgtttt gcattgagct ttgacaatgt atcaataaaa ggcactgcct catcagagcg
    31021 gtcattcacc aagtcaccgg taaaaaaaat cacatcccct tttaatgtgt taatcattga
    31081 aacagctttt tcaatatgtt ttgtatcgaa aaagctgccg gtatgaatat cagaaatctg
    31141 cacaattcta aagccttcaa atgttgaagg taattttgga aacttaattt tcctccgttt
    31201 gacctgataa ttatacttac catctcccat gccatacaag agagaaataa atggaattga
    31261 tgaaattaca aatgccattc gcactaaaaa ctgcgatctc gatacaacaa tcttctcttc
    31321 aggatttaca atttgtggag aatcataaaa tcgctgatat aaacctgaaa atccccatct
    31381 gactaatctg gtaagatcgt caatcaacag aaacaaaaca acaaagagtt ttgttattaa
    31441 aattatcata acagcagcaa agagataaac tctcactgcc tttggccatg tatgccaatc
    31501 tgtaatcagt ccggctataa atactgaaaa tgtaaacact gtaaaaaacc aataaatacc
    31561 tgaaatcaat cttgacattg agggagtcaa atccttaatc aataccttta taccctgaaa
    31621 aacataaagg tctatcagaa aaacaattaa taaaaaaatc aatactcttc gcaacataaa
    31681 aacgcacaaa tgtaagtgaa actattcatc aatacatttc tatcaattaa acttttctat
    31741 atataattca caatgaaaga tacagcacaa ccaacaaaca aactcataca tgaagacagt
    31801 ctgtatctgc agcagcatgc ccataatcct gtacattggc taccttggtc cgttgatgca
    31861 tttgaaaaag caaagactga aaataagtta cttataatca gcattggtta ttcaagctgt
    31921 cattggtgtc atgtgatgga gaaagaaagt tttgaaaata caactattgc tgaagtgatg
    31981 aatgaaaact ttatatgcat taaagttgat cgtgaagaaa gacctgatat agatgcagtt
    32041 tatatgacag ccgttcaatt gatgaaagga caaggtggct ggccactaaa ctgtattgcc
    32101 ttacctgacg gcagggcaat ctatggcggc acctacttca atccacaaca atggttgcag
    32161 gtgcttgaac aattgtcaat gatgtatgaa caagataatt caaaatttct ggaatatgct
    32221 tcttcattgc aggaaggcat gttacaatta gaaaaaattg aaacaacaaa tactaaacct
    32281 gattttgata ttttaaaaaa ttcaatatca aaatggtctt cactctttga caaaatatat
    32341 ggcggccttc aacgagcacc aaaatttgca atgcctgtta acctgaattt cctaatgcaa
    32401 tatggtcatg tttgtaatga tgttgaagtg caaaatcacg tcagattaac attgaacaaa
    32461 atgtacaacg gaggtatttt cgatcagctt gatggaggat ttgcacgtta ttctactgac
    32521 agtcgttggg aaattccgca ctttgaaaaa atgctatatg acaatgccca gctaataagt
    32581 gtctatgcac aagcattcag agcatataat aacatttctt ttaaaaatgc tgctgaagaa
    32641 acaataaact tcctgaacga aaaaatgaat gacaaaaaag gattttatta ttcagcaata
    32701 gatgctgaca gcgaaggtga agaagggaaa ttttatttct ggacaaaggc agaaatcaga
    32761 gcaacaataa atcaactgca tttagaaaac atatcaaaag aaaatgcttt tattattgca
    32821 tgccattact tttcaattcc agattcaaaa aaagaaaatg atgaaatagt tctgcaatgt
    32881 aacactgcca tattcaatga cagtagtgtt caaatgattg aacccaatca acagaaagaa
    32941 ctcattgata aaataaaatc agccatgaaa atggcaagaa gtgaacgcat tcatccggtt
    33001 tgcgataaaa aacttatcac ttcatggaat gccatgatga tatcggcatt agcagatgca
    33061 gcattatatt ttaatattaa agaatatgaa ttgcaggcaa taaccacctt caactatttg
    33121 gaacaaaacc tgtttataga agaaggacga ctacttcata gctccggaac aaaacaaaac
    33181 agcaatcttc atttagagga ttatgcactt ctcactcaag catgcattaa actatttgag
    33241 tgcaaatggg actttcagtg gattgagaaa gcaatttctt taacgggcta cgctattcaa
    33301 tattttagtc acgatgataa tgttttattt tatgactata cagcaaacga ttctgttctg
    33361 caattcagaa aaactgagct tcaggataat gttattccct gtgcaaattc agtaatggcg
    33421 aacaatttat tcttactttc aaaatacctc aacaatgaaa attatttgtt gcgatcaaaa
    33481 aaaatgcttg aaaaagtaat tgatcaaatt ccaaattatg gatttggtta tgcaaactgg
    33541 gccttactat tacaaagaat aaatcaacca ttctatgaag tagcttttgg tggttacaat
    33601 gctttaaaaa ataaaaataa acttaatgag tattttctac caatgaaaat cactgcacca
    33661 ataattctaa aagataaaat tgaatttact gctgataaaa atgaaactga tgacttgatt
    33721 tatgtatgca gaaaccactc gtgtttactt ccaacatcag atattattaa agttgcgcaa
    33781 actatttctg accaacaatg aagcaaaacg tattcttact ttgtgtaatt ttaaatatta
    33841 caataacggg gtgtcagatt tttaaaatta acaaatcaga caaacatgga aaccggcaag
    33901 gacattggga gcattattgg aatgatgagg ggaccattca acgaaagggg aaatacaaac
    33961 acggcttaga ggtaaaaaaa tggaaatatt acgatttaaa tggcaaactg ataaaacaag
    34021 aaaaatacag taagaaaaga gatgtcatta atataaaaac atattatgac aacaaaaaaa
    34081 taaagtctaa tggtaaggca tttttaatca atgaaaacga tagcatacta catttttact
    34141 ggcagggacc atggaactac tactcaccta caggtgtgtt agaaaaaaca gaatggtatg
    34201 aaaaagggaa aataaaaaag gctgtcacgt acccataaca gcctttaaaa ctctaaatat
    34261 atgtcatgaa attacaagct ccattttgaa atgcggaata ttagcttcat agaatagctc
    34321 gctaacaact ttaaatcctg ctttttcata gaatctaaca gtggtagttt gtgcatgcaa
    34381 gtaaactttc ttacctattg gctttacatc gtttaacaac ctgttaacca acatacggcc
    34441 taaacctgaa tttctgtgct cttttagaac gctgaatctt tcaagcttaa ttccctcatc
    34501 agtatgacgc catctggcag tacctgcagg aatattttta acgtaagcca aataatgttg
    34561 gctttcatgc tcaaatgtat cgaactcaat cattgggtct atatgttgtt caagaacgaa
    34621 cacccttcgt ctgatctcat ggatgagttc tgcaatatgc ttctcggaat aattaatcgg
    34681 aaacacatcc attgcggcat tgggctgtga gagattttca aatgtagttg cggtcatatt
    34741 taaagtttct tcggccataa aaatagaaat taattcttaa ctaatttttt gattatatta
    34801 ttaaaggaac ccctgatgct tagcatgtaa attcctgtat taagttgtgg acaatccatt
    34861 tcaatttctt gtatgttgtt gcttatgttt tgcacttttc gtgccactgt ttgtccgttt
    34921 tcattaaata gaaaaatctc tattttatca ttgtaagaag attgtatttt tataactgtt
    34981 ttatggtcac tgaaagaata gtataatatt cttatcgggt cggaaccaca gccttttact
    35041 gctattgtct tcagctcatt tctctttcca tcatagtcaa tttgagctaa cctgtaatag
    35101 gtaacctccg aatatggtgc ttcatcctta gtttcataat tcattaatgt attggtcgta
    35161 cctgcacctt taatggatgt aaggttttcc catttttttc catctctgct tcgctctaaa
    35221 ataaagatat cattattgag ctgtgtggca acagaccatt ttaatatcac actctgactc
    35281 tggcaattgg cgccaaaact taatatttct acaggcaaag gacttgactg actgcttagt
    35341 gtccaccatg aatttaatcc ggtaattccg ttaacgtctg tttcgaatgg tgatggattg
    35401 gattgcgaac ctttaaatga ttcccatgca tcgttaccta tccatcgctg agcacgaggt
    35461 gttgatatta cccctttttc aataggtgca taagtgaatc tcaaattagc attaggattt
    35521 ccaaccgctc taacatacca atagcgatca attgtttgta aactattgtc agcaccggaa
    35581 attgaacgga catgtgttac ccccggagga aatggtgtat tatcaggcaa actcctgtat
    35641 gttgccaatg aaacccaatc acatccactt gaacattgat atttgaatgg tatataagtt
    35701 gtgctgttaa aaccaaatgg aatttcataa ttaacactct gttgtgatga aagatgaatt
    35761 cttgcactac cgtcaatagt ttcacttctg atataacctg aagcatgtaa aatctgacag
    35821 ccattttcta acacaattga aagtccattt gtatttgcga tatgatccgc attaagattc
    35881 aattcaccgg aagtaagcat taatccagag cgaacaaaaa ccacactgtc ggcttcgggc
    35941 tttacgaagt taatattgtt tactccggca aagttaattt gtagtttgcc atatttattt
    36001 gaaactgcat tgactatacc atttggaata cctgtgatgt tctgattttg ggttccatta
    36061 tattcaataa tgctttgagc atcgagccaa aaattcattc tgttattacc tgcaatgagt
    36121 gtatttattg ttgatgccgc agaagttgaa taaaaaccat ttggattcat tgttttaatt
    36181 ttaccggttg caccaactga aagttgcgaa tagtaatttc cttgttgacg tgccataatt
    36241 ctctgtaatc ccatatcaag cacaccatta attgtaaaca cattatttac tgccggtgct
    36301 gatgaaacat gtgaagctat cagcaatggt tgaatggatg atttcacatc aagtgttgtt
    36361 aaagacccta caaccatttc tacctgttgt aaatcgtgtg ttgaagttgc tcgttgaaat
    36421 gtggaaattc cattcttatt aaacaaaatt tttccaaata ccgtgtttgt tgtaagatga
    36481 ttggcatgag taataattcc atttcctgaa agtaaaatac tatcagagtt taagattaat
    36541 acattttgac ttatatttga tgacccttgt gcattatcga agcgcaaaac tccgttgctc
    36601 tgactaaaat ttccttgtac aaatacacta tcagaaagtg atgtattacc ggtgcttgcg
    36661 tgaaaattaa aagtaccacc agattgtaaa tattgtcctc caatattaag ctttgcagcc
    36721 gcatcctgat aagtcagatt ctgtgtgcct ccagtttgaa ttaaattatt ttgaatattg
    36781 taatcaacat taccttcatt aacagaataa taaaactggc cgcccgtctg agtcatgttt
    36841 ccattaatta atactgatat atcatgctgc tgtgatccta aagaagttga gccgccaatt
    36901 gcattattac ctccctgaat ttcaaaatca ccatttatta caatattttc ttctccacgt
    36961 ccaacattac tcgcaaaaat tgcactgctg tttccactga tttttaaatc accattaata
    37021 actaaattga gttcaggatt attccaccag cctgtattgc taataagaac aaatctgtca
    37081 cttgaatttt gaaaattaat atttacgtct gatcctattg ttatagaaac tgtttttcca
    37141 tcggtgatat aactgtcaaa aactttgaat gttcctccgg aaaaattaag gctgtcaata
    37201 aaaacagaac atgctgcata attaccaggg aatggatgaa aaacgactcc aaaatttgaa
    37261 ttcacttgat taaatattcc gcttgaaata aaactaaccg ggccacgatt ccatgaaaca
    37321 ttaaacatgc ctccgtttaa gctacaatta ccatctatca caaattttac tgagtccgtt
    37381 ccatagggat aaaactgcga cactaattgt gttgacgacc cacttacatt taaatttcct
    37441 gaaatatgag tatttaactt tccacttgtg aaagccaatc cgacatttcc atttgaaaca
    37501 gaaaaatctt ttagcacatc aattcgttgt aatgcattat tggtaaaatt attactgaaa
    37561 acaaataagc tgcttgctgt ataactaaaa ctcccattaa tattaaaatt tgttgagcca
    37621 tcagaaccct gaagataaca actctctgtt ccgcttattg aaaaattatt ggtagtaaaa
    37681 tcaagattta ttgtattata atctgctagt cgaaaccagt taccggctgt cagattacaa
    37741 taagtattcc ctgcaacctg aataacgcca ttaccatcaa catatctgaa gaaatcaact
    37801 ttccctttta caacattcaa atctcctcca atactaacag tagcattaaa tgcattatag
    37861 cctgttgtac cttcaactgc actaaaatca tggcttagat aaacactacc tgcattattc
    37921 cacaacaaag taccatacgt tctaaacatc aatgctgtta ttgcattgct tttactgcta
    37981 tcggtaaacg acagtgtatt taatgtcagg tttctattag caccttgtgc gaaaataatt
    38041 gcacctgttt tatatatgct tacatgatta agatttaaaa gtgttgacat acctgtacca
    38101 tcatccatca caacctgacc attcgttact gatacatttc cctttatttt tgatggagca
    38161 aacagtccat cctgttgcca tactgcatta tagtttaata taagattacc gactgtcgaa
    38221 ctgacaccgg acataaaagg cacagtggct gcactccagt tattaacttc tatggtagca
    38281 ttgtcatcaa tagcttctgt tccattaaat attgttgttg ctgcatttga actattgttg
    38341 tgtacgtacc aagcatttgc ctcaatggtg agattactgt taactgttac aggtaaatta
    38401 gcagtgagtt ttgctcctga ttttattatt agtgaagcta aggaaggtgt cactacatct
    38461 acattaatct gatgatagat tattacatta tcagtacata acggtatctt gccaccttgc
    38521 catgttgccg gattgctcca tgctccattt gctacagaag ttactatctg ccctgtaccg
    38581 gcacttgaca atgtaccgtg attaattcct gcgcctgcta catcacctgc tattgtagct
    38641 gtgccacccc caggggattt acgcagtata ttcccggata cattacttgt tgatgcacga
    38701 gcataaatat tggaaattat taattcgtca gcctttggga ttccactgac agaaattgtt
    38761 actgttatgg ttgttaatgt tacagcaatt gatgctgttg taatatttct tgatgctaaa
    38821 aaagaaacat ttcctgatcc gggctgaaac ataaatccgg taggtgcact taggattaag
    38881 gttttattcg actgaatagc aaaatcgcca ttggcacctt cggtaataac aatattacca
    38941 atgggaagaa atgccgatgg tgtgacattt aggcaattac cacttgttgc tggcgaaacg
    39001 gttactgcgg cattaacact gacatttaca aaaaacaaca tcaggattaa ataactaatt
    39061 cgggacataa ctgtagtttt atcccgtttt ctacgataaa attcaaaaaa tgttccgaaa
    39121 aaatgctgta aaaactactc tgaaaattta tgaactaatg taacttttct cttttcagca
    39181 gataagctaa taaaacctga tgataacctt catggatgtc tgcctgaaca aaatcaattt
    39241 tatattgcgc acatttatgt acaagcatgg atttgtattc tttcatagct tcaatatact
    39301 tttctctcac caagttactg tgcactttta tctcttctcc ggtttcaaca tcaacaaagc
    39361 ggtatggtct gttttcgaac tgaaagtcaa gttcatgttt tttatcagta acatgaaaca
    39421 gaatcacttc atgcttattg tgttttaaat gttgaagcga tttaaacaaa gcttcattgc
    39481 tatctatgtt ttcaaacata tcactgaaaa taattatcag tgagcgtttg tgaatactct
    39541 ctgctaactg atgtagtgca tctgcagttg cagttttacg gtttacgtca gttttgtttt
    39601 ctaataattt ttcgagatga gcaaaaacaa gtttgtgatg agctgtactg gattttgcct
    39661 gagtttgtac atcaatctgc tctgaaaaaa ctgtcagccc tattgcatca cgctgttgct
    39721 tcatcatgta taaaattgca gcagcgctat aaacagaaaa tgtaatttta ttctgtaaac
    39781 tctttccacc tttcacttca ggatagtgca tagaggcgga gttatctatt acaatatggc
    39841 agcgtagatt ggtctcctct tcaaatcgct tgacaaaaag tttgtctgtt cttccaaaaa
    39901 gcttccaatc aatatgtctt gttgactcac ctgtgttata gagtctgtgt tctgcaaact
    39961 ctacgctaaa accatgaaaa gggcttttat gcaaaccggt aataaatccc tctaccacct
    40021 gcttggcaag caattccaga tgtgagaggg attgaacttc ctgatgatta accggcaaaa
    40081 tattgttcta taaaattaaa actacactaa ttgtgtatca atttttttgg caagaatatt
    40141 ttttggagct gcacctacct gcttatccac aacctgtcca ttcttgaaaa ataaaattgt
    40201 tggaatgttt ctaacgccaa attgcattgt aacattagga ttgctgtcca catcaatttt
    40261 tccgattact gcttttcctt cgtaatcttt tgccaattcc tcaacaattg gtcctaccat
    40321 acggcatggt ccacaccatt cagcccaaaa gtcaaccaat acaggtttgt ctgatttcat
    40381 tacaacttcc tcaaagttgc tgtctgtaat ttctattgcc ataattatgc tatgtttttt
    40441 atttgatatt tcagaacaat aatagtggtt taaagtttta tgatttttaa agatattgat
    40501 attttttcat ttttttaagt ctcgagctaa tttttcgccc atcaatagct aaaactcatt
    40561 gcttttgtaa cttaagttac tatttaatga gacttacacg ctctgaaatt gagttcagtt
    40621 ccttaaacaa ttctttattg tattctaatt tcagctgatt tgactccaca tcaatactca
    40681 gattttgatc attgtcacga atactgaatt tgacagttag tttgccctta ctatttttcg
    40741 aaatctcttt taactttttt atattcatct cattgattga ctccgaacga aatgaaatca
    40801 ttattccctt tgccattttg tttgccacct catccagaaa atgaactgaa ttaattttta
    40861 attcaaactc cttctcatta taaaatttcg tctgtaccct tgctttgata aaaacaaaag
    40921 catcgttatt cagtaggtgt ttgaatttgg catactcttc agaaaaaaca gcaaactcaa
    40981 aagaagcatt ataatcttca atagtaaaac tgccaaaagg tttacctgtc ttgctgactc
    41041 tatgattggc attggtgata atgccggcaa aatttacatc ccggttatta agtagtttga
    41101 gttcgttaag ttgttcgaca gagatattac aaaaagtttt tatttcttct ttataatcat
    41161 caagtggatg accggaaaga taaattccga ttatttcttt ctcctttttt aattgttcca
    41221 gattactaaa atgttctgtc ggaataattt taggcttaga tgcatcaaca ccacctaaat
    41281 cgccaaacaa ggtgtgttga gtgctgcttt ttgattcctg attagcactt ccaaatcgaa
    41341 taattttttc taactgcgtt tgattatcct ttgggtcaat tgcaaaatac tgtgctctgt
    41401 gaaatgatgt atcaaaatcc aatgcacctg caagcgctaa attttcaaaa cactttttat
    41461 ttgcagctct cagattcacc ctacttacta aatcataaac atctgtaaat actccaccct
    41521 tttttcgctc acttaataag ctttctgcag cggcttcgcc aacacctttt attgcagcta
    41581 agccaaatcg aatttcacct tttttattta cactaaactg taattcactt tcattcacat
    41641 cagggcctaa tacaggaata ttcattctcc tgcattcgtc cataaagaat gtaattttat
    41701 ctaagttgtt caggttgttg gtgaggacgg cagccatata ttcactcgga taatttgctt
    41761 tgaggtaggc tgtctgaaat gcaacaaagg cataacatgt agagtgcgat ttattaaatg
    41821 catagctggc aaaagcttcc cagtctgtcc atattttttc gagtatcttt tcatcatggc
    41881 cattgctttt tgcaccttcc ataaatttac tcttcatttt attgagcact tcaatctgct
    41941 ttttacccat agctttacga agtgtgtcgg catcgccttt tgtaaaattg cctaactttt
    42001 gtgaaagtaa catcacctgc tcctgataaa cagtaatgcc ataggtttct ttcaggtgtt
    42061 cttccatttc cggaagatca taggtaactt tttcttttcc gtgtttacgt gcaatgtaat
    42121 ttggaatata ctccattggt ccaggacgat aaagcgcatt catggcaatg aggtcttcaa
    42181 gcatatcggg cttgagatct ttcagatgct tctgcatgcc tgcactttca aactgaaaag
    42241 taccattggt ttctccgcgc tgataaagct caagagtacg aatgtcattc agcggtattt
    42301 catcaagatt aatttttaca ccatgatttt tttctatcag cctgacggca tcacgcaaaa
    42361 tggttagggt ttttaatcct aagaagtcca tttttataac tccggcatct tcaattattt
    42421 ttccttcgaa ctgcgtaatc agcaagtctg tttcttttga agtagaaaca ggaataatat
    42481 ccgtaaggtc tgttggcgca atgataattc ctgctgcatg aatacctgta ttgcgcacac
    42541 taccttcaag taccatagct tctttcaata ctgatgattg taatgatgtt ccttcttcaa
    42601 gaattgaacg tagtgatttg actctctcta attcatcagc acttaaattt tcttttgtct
    42661 tcagactttt ttcgccatca ataggtgctg tcagcaaacg acccaatgaa attcccggac
    42721 gatcaggcac cagtttggct aatgcattag aatcaggcaa cggtaaatca agcactcgtg
    42781 ccacatcttt aatactcatt ttcgcagcca ttgtgccgta agtaataatt tgtgctacct
    42841 gattttttcc gtacttgtca actacataat ctattacctt ctgccgacct tcatcatcaa
    42901 aatcagtatc tatatcaggc attgattttc tgtctggatt taaaaaacgt tcaaacagta
    42961 aattatactt aataggatca atgttggtga tgccaataca ataggcaact gcagagcctg
    43021 ctgcactacc tctgccgggt ccgaccaaca caccaatatt tttgccatga ttaataaaat
    43081 cagcgacaat taaaaagtag ccggcaaaac ccattgtttt tactgtaaac aattcaaagt
    43141 ttatgcgctc ttcaatatca ggggtgattt cgctataacg tttgcgtgca ccttcccatg
    43201 caagatgttt cagataatca tcctgacttt cgaagccttt tggaatctga tagtgtggta
    43261 aaagaatatc ctgcttcagt tttaatggct caattttatc tacaatttca ttggtattgt
    43321 caattgcttc aggtctgtca ctaaaaagtt gttgcatttc tgatgtgtct ttaaaataaa
    43381 actcatcgtt ataaaaagca aatctcttat cgcgtgcaga tgtatcttca tcgccatctt
    43441 taaatttttc tgtgctttgt ttctcaccgg tgttgatgca aagcaaaatg tcgtgtgcat
    43501 tatagtcttc acgatttaca taatgagaat cgtttgatgc aataattttc acattgtgtt
    43561 ttaatgcaaa cccaataaga acttcattaa ctgtattctg ttccggaata tcatgacgtt
    43621 gtaactctac ataataatct tctccaaaaa tatcgagcca ccataaaaat tctttttctc
    43681 cttcggcctc acctttgcgt aaaattgttt taggcacact tgcacccagg caacatgttg
    43741 ttgcaataag gccttcgtga tatttaagca ccaattcttt atcaatacgt ggatactttc
    43801 catacaaacc ttccatataa cctagcgagg ttaacttgac aaggttttta tatccaatag
    43861 tattttttgc aagaaataac tgatgataac gtacatcttt atcgtctttg gtaaagcttc
    43921 gtttatgacg attttctacc agataaaatt cgcaacctac aataggttta attaaattgg
    43981 ggttttcgct agtattgtat tttgcagctt cggcaacaaa attaaataca ccaaacatgt
    44041 ttccgtgatc ggtaattgcc aaagctggca tattatcttt tactgctttt ttataaagcg
    44101 acttaatatc tgctgcacca tcgagcaaag agaactgtgt atgattatgt aaatgagaga
    44161 aacgaggcat gaaaaaactg agtatgaatt ttgaatccaa taaccggttt ggctattgtg
    44221 gcatgtaaaa ttaataaaac gattcaaaaa ttctttgttt gttaataaca aaaacccatg
    44281 tgactttcag agatgtgcat gccctaaaat gaaaaagcaa ccgatgttgg cacataccgg
    44341 ttgcttttat ttgaggttca cttttgtttt agaccataag caaatgaaga ttattgttat
    44401 gccttaaaca tttcaatagc catgccaata atattgtgtt gtaagaaaga tgtaagaaga
    44461 aatgaaattc aactatgcag gtttgctttc tttgaccaat tcagaataaa attttctgct
    44521 tttcaggtcg taacaattac cttttgtgag tacatgaaca atcagatttt caatgcttat
    44581 gggcacacca tcctgaaggt cggcaatatt gttgtggcga atctgatgcc cgtcaattaa
    44641 tataaccaat cccgaaccta taatttcgag caaatgacct tcagtgagca gtactcctgt
    44701 atcttctcca agaccgatac caatacaact tgggttactt gctactgcct gtgcaagtcg
    44761 gccaaatcgc ccacgtttat cgaaatgaga atcaataata acatcgccca tcaatgcaag
    44821 tcctgtagta attttcacct cacctttaat taaagcacct gaactgctac cctgataaat
    44881 catggtatta ctcatagcca tagcaccggc actggtacct gcaattacaa aattttcatg
    44941 ttgatatctg tgctgtataa tctcaagaat ctctgttccg ccaaaaatgg tactgagtcg
    45001 taattgattt cctcccgtca tcattacccc atcacacttt tttattcttt caatatattc
    45061 tgattttgtt gcatcttctc tgttgcgaat gtccatcaca tttacattag tacatcctaa
    45121 cttaccgaat gcatcaatgt agttttcacc aacttcaaca gggatgctgc tggcactggt
    45181 gatgacttca atcttagcat tttttcctcc catttcctga cagatgcggc taagaatttg
    45241 taaagcaaaa aagtttaagt tattcttctg ttcaaaatta ggttcggact ctgttccttt
    45301 atcttcattg ccaccaatgg caataagt
//
LOCUS       DAOOOL010000004        43975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_55_length_43975_cov_184.992646, whole genome shotgun sequence.
ACCESSION   DAOOOL010000004 DAOOOL010000000
VERSION     DAOOOL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 43975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43975
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_43975_cov_184.992646"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKU82_00680"
     CDS             <1..679
                     /locus_tag="PKU82_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ81416.1"
                     /translation="SNINLHVGKGEVFGVFGPNGAGKTTLLSLVTGILTCRLGVISIL
                     GKDASTQRKNVNQIIGYVPQDFAFYEELSAIENLIFFGAMYGLKEQALDNRAKELLEE
                     FGLMNVAERYVKEFSGGMKRRLNIAIGLLHKPQLLILDEPTVGVDVQSRLAIMSYLKK
                     INSEGTTIIYTSHLLKEAEEFCSRIALIDKGKVIAIDTIANLLIQNQCKSLEEVFIKL
                     TGNNYRD"
     gene            679..1950
                     /locus_tag="PKU82_00685"
     CDS             679..1950
                     /locus_tag="PKU82_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ81417.1"
                     /translation="MSPLVASIIKEFKVLSRDKTGLLLMFLMPLVLALLMTVLQNSAF
                     KIVNENKVDLMLINNDSNSLFFQNFCKGIEQTGMFDLKINTKEISSKKLSEDKESIVV
                     VTIPKGYAYTLDQEVKAKAEQSLQMFGMLESVSTKGIIGVKNIELSFDPVLQESYRFS
                     IKSTVLSVVMAIQNEMLLKQLFYDVNQSEPDSAALSKILPKPLLLSESLIKENSVANP
                     NATQHNIPAWTVFAMFFTVISLGGNIVREKRSGSFLRIKTLPISITQMLIAKQVVFWI
                     VTVLQTLVLFSIGILIFPMLQLPPLVISSGLLPLFLATLCCGLCAVSFALCIGVYSDT
                     VEQANGFGAASVVILAAVGGIFVPLFAMPEVFKLIANVSPFSWAMKAYYSAILEISPI
                     HKLLLNVLPLLLLTAILQLIAFIGLKRKNLL"
     gene            1947..2216
                     /locus_tag="PKU82_00690"
     CDS             1947..2216
                     /locus_tag="PKU82_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOZ81418.1"
                     /translation="MTKEEIKPELKKHIIKYLNLLDLTPESIKDDQPLFGEGGLGLDS
                     IDSIELIVLLEREYGIKITDPKEGRKILIDVNTIADYIIQHKSAK"
     gene            2228..3421
                     /locus_tag="PKU82_00695"
     CDS             2228..3421
                     /locus_tag="PKU82_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ81419.1"
                     /translation="MENVCITGVGLVTAVGNNANETILAIQSGKTGIGIPKYVKGRYA
                     SMIPFGEVKLSDMQLADELSIRNKVVSRTQLLAHKVFNEALNNSALHIDDLKNTETSI
                     VVSSTVGGMYLTDELYADSNNLSKGSDYLSLYDCASIGIYLQQLTGAKGVINTINTAC
                     SSGANALIYGMRLLKNKRAKRVIVGGVDCLSKFTINGFNALNILSTRSCRPFDNNRDG
                     LNLGEGAGFLILESSDTAKEEKVLAYIKGYANSNDAFHPSALSDEGFGPMAAMRGALE
                     SAGLMPKEINSVIAHGTGTPNNDRVEGLSIKRIFGESLPPVCSLKSILGHTLGAASAV
                     ESVLAVIGVRDGHVYPSRNFETPDEETGLVPNLNFKKEKIKHSLLNSFGFGGNCSSLI
                     FSSVN"
     gene            3452..4462
                     /gene="obgE"
                     /locus_tag="PKU82_00700"
     CDS             3452..4462
                     /gene="obgE"
                     /locus_tag="PKU82_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOZ81420.1"
                     /translation="MEKNFVDYVKICCRSGAGGPGYVHFHRDKKTAKGGPDGGDGGRG
                     GHIIVRGNKQLWTLLHLKYRKHILADAGQKGGNALRTGADGKDEILEVPIGTIARDFE
                     TNKFLFEIEEDGQEVILARGGRGGKGNAFFKTSTLQTPRHAQPGGEGIEEWKILELKL
                     MADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPNLGMVYYHDDRSFVMADIPG
                     IIEGAHTGKGLGLRFLRHIERNSILLFLIAADAKSIKEEYKILKNELRQHNPELLDKR
                     RVIAITKSDMLDEELLKELKKEVPNDKNPGGGKVPVVFISSVANFNIDKLKDVLWKEL
                     NS"
     gene            4459..5022
                     /locus_tag="PKU82_00705"
     CDS             4459..5022
                     /locus_tag="PKU82_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOZ81421.1"
                     /translation="MIEQIKEFDTNLFLWLNSIHSPAMDSFFFAITNRFVWIPVYLIL
                     LFLLIKKKKNKTWSILLFIVILIICSDQLSVLIKENVMRYRPCHNLNIQGVVHTVNNK
                     CGGKYGFVSSHATNTMALFAFLVLSVFKKERIVIALLAIYVLLVSYSRVYLGVHYPFD
                     ILGGWITGLAVALVLYYFVYNKLFNQP"
     gene            5058..5432
                     /gene="crcB"
                     /locus_tag="PKU82_00710"
     CDS             5058..5432
                     /gene="crcB"
                     /locus_tag="PKU82_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOZ81422.1"
                     /translation="MRTILTIGFGSFIGGMIRYMLSLFIQSKTVSEFPVGTLLVNIIG
                     CFLIGVVYSFAEQGKMAVEWRLFIATGILGGFTTFSAFSMESFMMLRNGYYGMMTVYV
                     FLSLAIGISATIGGFLLVRTLN"
     gene            complement(5486..6121)
                     /locus_tag="PKU82_00715"
     CDS             complement(5486..6121)
                     /locus_tag="PKU82_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOZ81423.1"
                     /translation="MIEPVISGFLLGCFLAILAGPVFFLIINISLRKGFSYASRVAIG
                     VLISDTLFIFIAYFGSNFILLLNNHRFIAGIISGILLIGFGIALILKRNSIPAEAITV
                     NEKTDSKSLYILKGFMINSMNPSVLFFWIGVSGTVSVKEHYTIHHLLLFYGSILITLF
                     SFDLFKAYIAHKLKSILTINLLTWLNRITGMALLGIGLYTLIVACWQAYAK"
     gene            complement(6141..7688)
                     /locus_tag="PKU82_00720"
     CDS             complement(6141..7688)
                     /locus_tag="PKU82_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOZ81424.1"
                     /translation="MTLIGRIIKASIDLRGKIEIVPFSPFISQKRVLKKLIRKAQFTM
                     FGEAYNFQGMLGNKNFVTKFRAAVPVHDYNSIFSNWWNKSLHDQENVCWPGKVRYFAL
                     SSGTSGSSSKHIPVTKDMSKAIQKASLKQILSLSKYDLPFSTFEKGILMLGGSTHLHQ
                     QGSYFEGDLSGITASQIPFWFQHFYKPGRKISREKDWHAKLDEITHKAKDWDIGIIVG
                     VPAWVQLLIEKILDHYKLKNIHELWPNLEIYVHGGVSFEPYKKGFSKLLGKQIYYIET
                     YLASEGFIAYQDEPNTRGMRLLVNNGIFYEFIPFNEDNFDADGELKHKPKTLLLNEVK
                     ANEPYAILLTTCSGAWRYLIGDVITFTNLEKCEIQIIGRTKHYLSLCGEHLSVDNMNR
                     AIELVSDELNIDIPEFGVAGIPYQSLFAHKWYIGCDTYNDSENLKKLIDEKLKILNDD
                     YRVERRSALKEIFVEIIPTSWFYKWMELQGKIGAQNKFPRVMKNKVFEDWEKFVASQH
                     KNINQAV"
     gene            complement(7785..12026)
                     /locus_tag="PKU82_00725"
     CDS             complement(7785..12026)
                     /locus_tag="PKU82_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81425.1"
                     /translation="MKDFQKIISFFMVTLIFVLSSYRSEALIFQSVASGNFNTLIWNQ
                     AGLYPTTGDTVVINTGTTVTINGFNTDVLGSVTINSGGNLTIANNAVAQLVFAGDLVV
                     SGTLINNGRINMTVPGNFVLGNGAVYDHNPKINIAVDENVFENGVENFSPTSTLIIRK
                     WASTALPLAGPTRVTSDIGNLVTSVTGTTWEQRGQFSPNRIKGSLLVTDGVLRMDDGS
                     GATTVLTLNDVTLRGNSAIIFQEGNNRNLTLTMGNFTDSSVLVNQPTIIMNMSLGTLN
                     LTVNGNFITRHDFIAVFDSTFPIRSASVTANIMGDLLIQGSGNKFDFCKQVNAPLTLT
                     VGGNTKIANNPNYVRFVDSGNGFLNYTTGDLNISAGLKNIFLGGNDLLDASNNPLYPL
                     PTGYATVNVLNDFNLSGVTNTTILLSPGNVARTKLIVGHDLNSSSASVNFVVANNIGI
                     VNIEIGSNLFLNGGSMNVQANASSNAIDSIAVTRSFIFNSTNPANFFRGNSGAGNTFI
                     RVLGDFNLMNSGTAANQGVCGTYLNNGNLEFYVAGNFNQSAGAFYGIQDGTGRNTFTV
                     DGTLNQSGGFFKGTHATNIFTAGKSIFTLGGFSFSGGNYIQYNAANDSVICTVNGDFN
                     VNYTAVTNLVSFIPYLHPVYSNTSPLKLTVTGDMYFGGANGTFYSSVTQGNELISIGA
                     NLTFDNGNNSFNNYPSAVITTTHNVNITVGGNLTCNNGINNFSAGRGDLAGTINGDLI
                     ISGGTLSLKSNSAYNPIIINVLGGFNMTNGNFFYTNNATIPPITTTVTINSDDDNTGD
                     FVHSGGTINFVNNTLNPTVQSLTIKSPNVLLGPSGTIKRNGAGTIASRGALDFARTGT
                     TLFTRLNGHTVDHTVLTVVNGTTLEVISGDVQLTSYVEEFAGQTLPMLYVSSGATFTL
                     RNNSSVYTTGNFLKSTVQGASGSRIRIQHTNGFYSGFSSAAINSSGNMGFLLSSSSTV
                     EYFGDDNQIITGIGMGNAVGGNQKYGVLEINFGGDPNTEYVYPTSNGTVNVRNQLKLT
                     AGQLRLDDDNDPTNGGRTIIIENSPNTSITRVAGYIRAETYDGASNVKWMFAADNTPH
                     IFPFGLDPTTYIPLTVTSTGGNAGFITASTFKTSPANLPYPPGVGHVNSVATGADNSA
                     DCVDRFWKINQSGTSTTFNAVFSAPVAEFPAIAGPYKAQHYNYVGGFWDNPFQGAQAY
                     ASAGPIRTVTATGVSVTNDWWVLVNQNTPLPVELLSFDANCVKNHHQIKWTTASELNN
                     DFFTLEKSADGTEFTLFRKIEGSGTVNDSKNYEVSTDDGIAAYYRLRQYDFNGTMHDL
                     KTIYAPSCQNDEFKLLSVNQVEDIVNSIVSSPDNIKAEITLFDLKGALIFKEEVSLST
                     GVNSVRLPINLAKGLYYFQIINDNQVTQGRKFLVN"
     gene            complement(12411..13076)
                     /gene="tsaB"
                     /locus_tag="PKU82_00730"
     CDS             complement(12411..13076)
                     /gene="tsaB"
                     /locus_tag="PKU82_00730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOZ81426.1"
                     /translation="MALLLHLETSTDTCSVCISDNETIIDCEETHNSKSHASLLAVFI
                     DELLKRNRLTYKNIDAVVVSCGPGSYTGLRIGVATAKGLCFALKIPLISVATLQSMAN
                     IANKQCKGQLLCPMIDARRMEVFTALLNPQLDFIEKENAVIIDEQFHHDKLVKNKICF
                     FGNGAAKLPDSIKINSNCVIFDEFKMSAMGLVTLAFKKFTQQTFENLAYFEPFYLKDF
                     VGK"
     gene            complement(13082..14458)
                     /locus_tag="PKU82_00735"
     CDS             complement(13082..14458)
                     /locus_tag="PKU82_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ81427.1"
                     /translation="MSKKNNTLRNIIIIAGVVIVALVIAKKAGWLGEENVTNVSTEKA
                     SLQTITESVSASGKVQPEVEVKISSDVSGEIVEMLVKEGDVVKKGQLLCKINPEIYIS
                     SLDRAVAGLNSTKANYESSKSRLSQAESQLVKAEIAYTRNKKLFEEKVISASDFEAIK
                     SAYEVAKAEVDASKQGVSGAQYNINSGSATVREAKENLNKTEIFAPVNGTISKISKKK
                     GERIAGTSFTDGTEILRISNLQEMEVKADVGENDIIRIHLNDTTLVEIDAYSPRKFKG
                     IVTEIANSPNESLTSTEQVTNFAVKIRILRESYSDLIPSDHPEYSPFRPGMSATVEIQ
                     TKKVSNVVSVPIKAVTTRDTTIRTSDKAVVRKNDDTPPDDKVVVSSNTNDTPVKEIVF
                     VNDNGIAKMHVVKTGIQDNNFIEILEGIKVGDEVITDPYQAVSKKLVDGDKIKVVPQN
                     QLFKEEQK"
     gene            complement(14468..15886)
                     /locus_tag="PKU82_00740"
     CDS             complement(14468..15886)
                     /locus_tag="PKU82_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ81428.1"
                     /translation="MKPFKSIFLFILFFSEANAQKIWSLKDCIDYALNNNISVQQSII
                     ASKIAAATYQQSVGSLFPSINASSSLNYNYGRSLDPTSYQYVDQTFQSGGASLNGNLP
                     IFQGLELQHNLRKSSIDFDVSKLDVRKVQNDIALSVAAAYLQILYSNEQLKATNDRIE
                     AVTKERNRAKILVDNDLLAEGSLLDADALLATEEFNKASAENLLTSSKLTLIQLMQLE
                     GANDIAIETPALEIPSQESLSLSSESVYNEALKNLPEVKASELRLKSAKESLGIARAA
                     RYPRLSAFGSLSTNYSNQATSLDGNPVFSGYVPSGAVTSSGEQVLQPVFNYKYIDTPV
                     KDQLNNNFGKAIGFNLSIPLFNGWATNSSIKRSKLSFENARLNALQTKNTVFKNVQQA
                     HADALAALNKYAAANKSVTAQEKAFLYTEKKYNAGLLNSIDYVNARNNYTKSQSDLIQ
                     SKFDLILKIKVLDYYLGKPLSF"
     gene            16099..17430
                     /gene="ffh"
                     /locus_tag="PKU82_00745"
     CDS             16099..17430
                     /gene="ffh"
                     /locus_tag="PKU82_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOZ81429.1"
                     /translation="MFENLSDKLEKAFKNLKGQGKITEINVAETLKEVRRALLDADVN
                     YKVAKQFTDTIKEKALGQNVLTAVSPGQLLVKIAHDELATLMGGQMTDIQLKGNPSVI
                     LMSGLQGSGKTTLSGKLANFLKTKKGKTPLLVACDVYRPAAIDQLKVLGEQIGVEVYS
                     EVENKNPVEIARHAIKQAKQNGNNVVVVDTAGRLAIDEQMMNEIAALKNAIEPDEILF
                     VVDSMTGQDAVNTAKAFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVTLVEKAQEQFDEKQAAELQKKFAKNQFNFNDFLS
                     QLQQIKKMGNIKDLVSMIPGVGKAVKDMDIDNNAFKSIEAIIQSMTPAERANPDLING
                     NRKKRIAAGSGTGIPEVNKLIKQFEDMRKMMKMMTTGNMKNMMRNMPGMRR"
     gene            17461..18342
                     /locus_tag="PKU82_00750"
     CDS             17461..18342
                     /locus_tag="PKU82_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOZ81430.1"
                     /translation="MTIIDGKQTAEEYKSILKQEVKALVATGKRPPGLAILLIGNNGA
                     SETYVANKLKTCHDVGFNASLFRFENTVSENEILNCINELNFNSSIDGVIVQSPFPNH
                     ISPQKINEAIHPAKDVDGFHPMNAGRLQQNKPCYIAATPKGIMQLIEKYKIETQGKHC
                     VVVGRSNIVGMPMSILLSRNSKPGNCTVTICHSKTVDLAAYTKRADILIAAIGSPELI
                     TADMVKEGAVVIDVGITRVADKATKSGYRLKGDIKYDEVAPKCSFITPVPGGVGPMTI
                     IGLLQNTLLAAKNAIYN"
     gene            18354..20153
                     /gene="argS"
                     /locus_tag="PKU82_00755"
     CDS             18354..20153
                     /gene="argS"
                     /locus_tag="PKU82_00755"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOZ81431.1"
                     /translation="MIFEQELASITNEALKVLFNFEPDESLLTYTQTRKEFDGDYTIV
                     VFPFVRQSKKSPEETGRLIGEYLIKHASFISNYNVIKGFLNITVSDEQWLKRIAVAFA
                     DSEYGFTKADKSKPVIVEYASPNTNKPIHLGHLRNILLGYSVSLLLKATGRNVKRVSI
                     INDRGVHICKSMLAWKKFGNDETPQSSGMKGDHLVGKYYVEFDKQYKQQVSEMISAGI
                     EKDKAEKNAPLMQEVRQMLIEWEQGNEEVTALWKKMNGWVYEGFDVTYNRLGVDFDKT
                     YYESETYLLGKKIVEEGLSKGVFYKKADNSVWVDLTNDGLDEKIVQRSDGTSVYITQD
                     LGTAVLRHDEFDFDSKIYVVGNEQDYHFKVLFLILKKLGYEWAEKLYHLSYAMVDLPS
                     GKMKSREGTVVDADDLMDEVTAAAAEETSKLGKVEEFSSDELKQLYETLGFGALKYFI
                     LKVDPEKRMLFNPAESVEIHGNTGPYIQYVHARICSVLKRASQMEQLSNYKNTAIESL
                     VSMNEKERELAINILRFKETVETAAEKFSPAIVANYVYELAKNFNQFYHDNSIISEGE
                     LETSQFRLCLADACAMTIKKSLNLLGIKAPERM"
     gene            complement(20172..20858)
                     /gene="cmk"
                     /locus_tag="PKU82_00760"
     CDS             complement(20172..20858)
                     /gene="cmk"
                     /locus_tag="PKU82_00760"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOZ81432.1"
                     /translation="MIIAIDGYSSCGKSTVAKAIAKRLKLNYIDSGAMYRAVTYYFLQ
                     NNIPFSKTADVIADYSSILENIQIEFIRNEQTGQSEIYLNGKNIEAEIRQMDVSDNVS
                     YVSAIKSVRKKLVAMQQQMSTLGNLVMDGRDIGTTVFPNADLKIFMTASPEVRVYRRY
                     HELIEKGIKVTPDEIRKNIELRDYEDTHREESPLRKPDDAIILDNTYMSRDEQVQFVL
                     DAVAKLKKAI"
     gene            complement(20933..21991)
                     /gene="porQ"
                     /locus_tag="PKU82_00765"
     CDS             complement(20933..21991)
                     /gene="porQ"
                     /locus_tag="PKU82_00765"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOZ81433.1"
                     /translation="MRITTLIAALLLPVCLKAQTGGSAIFRFLDLPSSARHAALGGNF
                     ISIRDNDLNTSFYNPAILNVDMDKQITFNYNPYFSGIKYGYTAYAHHIENIGTLSAGI
                     QYVSYGKFDRTDETATDLGTFSGGDYALNISMSRPVGIDSVLFVGASLKFIYSHIDTY
                     SAFGIAADLGVNYYSPDKLTSAALVIRNAGRQLKSYTEKNNEPLPIEIEAGITQKLAK
                     APFRFTLTYRHIEKWDLSYIDNNSQETDPLTGEVKEPEKASFGNKLLRHLIISNEILI
                     SKNFHLRMAYNFQRRNEMKVESRPAMVGISFGLGLKISKFHLSYGRSLYHLAGGTNSL
                     SISTYLNDFKRKPKTTDS"
     gene            complement(22056..23324)
                     /gene="glyA"
                     /locus_tag="PKU82_00770"
     CDS             complement(22056..23324)
                     /gene="glyA"
                     /locus_tag="PKU82_00770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOZ81434.1"
                     /translation="MQKDKAVFKLIKEELARQRHGLELIASENFVSQQVMDAMGSCLT
                     NKYAEGLPGKRYYGGCEVVDEIEQIAIDRAKTLFNATWANVQPHSGAQANAAVMLACL
                     QPGDTILGFNLSHGGHLTHGSPVNFSGKLYRPTFYGVEENTGTIDYNKVEETALKEKP
                     KLIICGASAYSRDWDYVALRSIADKVNALLLADISHPSGLIARGLLNDPLPHCHFIST
                     TTHKTLRGPRGGLIMMGKDFENPWGLKTPKGETRMMTALMDSAVFPGTQGGPLEHVIA
                     AKAVAFGEALSDEYFEYCLQVIRNAKTMANAFVKKGYKVISGGTDNHLMLIDLRSKNI
                     TGKQAENALVRADITVNKNMVPFDTQSPFVTSGMRVGTPAMTSRGMKETEMARIVELI
                     DMVIMNHTNGAVIEEVKTTVNDMMEHFPLY"
     gene            complement(23360..24649)
                     /locus_tag="PKU82_00775"
     CDS             complement(23360..24649)
                     /locus_tag="PKU82_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOZ81435.1"
                     /translation="MKYNSINSKLFVDNRKKFTAELKPKSIAIFQSNDEMPRSGDQNF
                     TFRQNADLFYLTGIDQEESILVIFPDSPLPQYKEILFVRKTNERIAIWEGHKYTKEEA
                     QATSGIKHIYWTEEFNAVVPMLMHHAENVYVNINENDRHATEVPYRDMRFAREWKNRF
                     PAHNFERLGQIMAKLRSRKHDIEVQLISEAIKHTESALRRVLKFMKPGVMEFEVEAEI
                     IHEFVRNRCNGHAYSPIIASGASACVLHYNENNKPCKDGDLVLMDFGAEYANYAADLT
                     RTIPVNGKFTPRQKEVYEAVLRVMKAATKLLVPGNFIEKYHAEVGKMMEEELVNLKLI
                     TKDEIKNQNPEWPAYKKYFMHGTSHFLGLDVHDVGNKYQAMQAGMVFTCEPGIYIPEE
                     SIGIRIENNILITDKAPVDLMATMPVEVSDIESLMRK"
     gene            24778..25947
                     /locus_tag="PKU82_00780"
     CDS             24778..25947
                     /locus_tag="PKU82_00780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ81436.1"
                     /translation="MLRIEVFNSVREMPANDWQTINGENVIFTSFSYLSALEQSKPAG
                     MQFRYVIVYDSKMPVATLCFQLLDVANKDLDGVLNLKDKGWLLQNLNKSINRLLFKCQ
                     TGKVNFLICCGSFFVSGEYGINVINRDALPAVIQLLPEVRQKITDTISNASVCGWMVK
                     DFYNNTKVDQPLLEDDFFELPMDPEMILYIREQWHSFEEYLASLTTKYRVKANSVIRK
                     IEDVDVVALNAAEVLKYGDELIRLYLNIQQKATVQMARVNILYFEKLLTIMPEKFYVK
                     VFFKESKVIAFYCGFHHAGHHEAHFIGIDYSLNKTLSLYQNILYRFIEEAISQRSSNL
                     YYGRTAMEIKSTTGAKAYPLHAYFNLSNTVLNKMVKPVVRRFTPEEWIARNPFRE"
     gene            26072..27808
                     /locus_tag="PKU82_00785"
     CDS             26072..27808
                     /locus_tag="PKU82_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81437.1"
                     /translation="MIRTIQNTTKAAIILIMFSYIGNPFPAYAKGKTLKATLKGQNVS
                     CFGGSNGSISLDISGGKAPYQITWNDGTSGYAMLDKPAGEYSVSITDSKGSTVSQSIT
                     LTEPYAMTIYSQVTSEQCYARNGKANLLVTGGTQPYQYEWSNQTRQQNLEAVAAGDYE
                     IKVTDKNGCIKQSNITIDRNKVLELNIAKYSPLCNDEASGLIDVEVKGGWSPYTYEWS
                     NGAKTEDQSGLKAGNYSVIVKDNNGCTAKAEVQLKNPETIKVEAKVTEADLNKSNGSI
                     MLQVSGGTGNYTYLWSNMESSQSLNNVEEGVYAVRVDDAHRCSAAAYFTVNEKSPMQV
                     ESVVKHELCNGGKNGSIKLNVKGGVAPYKFQWSNGMNGATISNLTASDYSVLITDANN
                     KVYRNTFTVKQPEPIGIFATVLDESYTGKNDGSIKLQVIGGMPPLKYQWSNGQTVADL
                     ANLSQGTYTVRMKDGNGCAMEHHVLVGINYTDAMLRTAFANEDNAMMEIYPNPFSNNF
                     TMKFSTPVNDIQSIIVYTMDGKIAQQLNPDLKQQQVNEIAVNASKLKPGNYLVKVTTD
                     KKIFTRIVTRTM"
     gene            complement(27900..28772)
                     /locus_tag="PKU82_00790"
     CDS             complement(27900..28772)
                     /locus_tag="PKU82_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOZ81438.1"
                     /translation="MQVLQKTSQYLSLVKFSHTVFALPFAAIGFSLAISQNGLPVFWP
                     LAIKVLVCMITARNAAMAFNRWADRSIDALNPRTALREIPAGIIKANRALWFVIVNAL
                     LFTVTAYLINKLCFFLSPVALFVVLGYSFTKRFTALCHLVLGAGLALAPVGAYLAVTG
                     KFDITPILIGVAVLCWVSGFDIIYALQDEQFDRENKLHSIPSLMGKKQALQVSRILHF
                     LSAGFLALFLYQAHVGLIAWSGLAIFCALLIYQHSLVSENNLSKVNIAFATTNGIASI
                     VFAGMVISDIFLLH"
     gene            28847..29434
                     /locus_tag="PKU82_00795"
     CDS             28847..29434
                     /locus_tag="PKU82_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HOZ81439.1"
                     /translation="MNFLAHLYLSDNNEPLMVGNFIADHLKGNDWKSLPEGIMSGVKV
                     HRFIDHFTDTHPVVEQSKVLLRPYFHKYTPVISDIFYDHFLAAEWDKYSNQPIDEYAR
                     YVYQLMHKNYQFLPARTKEMLVYMENDNWLKNYATLNGMHRALSGMGRRAKFDNNMNQ
                     AAEFLYDNYELFEKHFKEFFPELESAVKKEISTQT"
     gene            complement(29421..30830)
                     /locus_tag="PKU82_00800"
     CDS             complement(29421..30830)
                     /locus_tag="PKU82_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HOZ81440.1"
                     /translation="MRYLDHFEKIAEKRIRDRHKHRYYWNDITFYCNYFSHETFSVLE
                     VGCGTGELLNNIRAAQKTGIDYSPTMIAEAKKQFPQIDFQVMAAENISLEEKYDLIIL
                     SNLTGYLEDVQAVLEQLKKVCHERTKIIVSYHNHLWEPFLRLAENLGLKTKTPQLNWL
                     TSMDVRNLLFLAGFDCYRESRRMLLPINIPLLSPLINKFIAKLPLFRLMCINTYSVAK
                     PLANATEDVNEKYSVSIVIPARNESGNIENGLLRIPKFGKWQEVIFIEGNSTDDTWDS
                     INEVSSKYAASHKIKVGKQEGKGKNDAVRKGFSMAEGDILMILDADLTMPPEDLPKFY
                     EAIASNKGDFINGCRLIYPMEKEAMRFLNMLGNKFFSQAFTWLLEQPFKDTLCGTKVI
                     FRDDYDRLVTNRKFFGDFDPFGDFDLIFGAYKLNLKIQELPIRYRERTYGSTNISRFK
                     HGIILLRMCFFAARKIKFV"
     gene            complement(30843..31499)
                     /locus_tag="PKU82_00805"
     CDS             complement(30843..31499)
                     /locus_tag="PKU82_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOZ81441.1"
                     /translation="MLDKNAIAKRIAREVKDGMYVNLGIGIPTLVANYIPKGINVTLQ
                     SENGILGMGPFPFEGEEDADLINAGKQTVTLLPGSVIFDSAMSFGMIRAQKVDLTILG
                     AMEVSEEGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCTLP
                     LTGIKCIKRVVTELAVLDVTPQGFKLLERAPGVSVEDIKKATAGKLIVEGDIPEMVID
                     "
     gene            complement(31557..31925)
                     /locus_tag="PKU82_00810"
     CDS             complement(31557..31925)
                     /locus_tag="PKU82_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOZ81442.1"
                     /translation="MSRKFDSPFEDNKILKLTFEFSLKIIHLSEILNSNHQYIIANQI
                     LKSATSIGANAYEAQNAESEPDFIHKFKIAAKETEETYYWLLLCKHAECYPDTTDLLI
                     ELEVIKKIINKIISTSKNKL"
     gene            complement(31967..32674)
                     /locus_tag="PKU82_00815"
     CDS             complement(31967..32674)
                     /locus_tag="PKU82_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOZ81443.1"
                     /translation="MNKVVKNVDEALKDFRDGMTLMVGGFGLCGIPENCIAALVRKGI
                     KGLTCISNNAGVDDFGLGLLLQQKQIKKMIASYVGENAEFERLMLTGEMEVELIPQGT
                     LATRCMAAGYGMPAIFTPAGVGTEVAQGKEVRRFSYNGVEKDYLMEYAFDSDAALVKA
                     WRGDTEGNLVYRSTARNFNPMMAMAGKITIAEVEELVPAGTLDPDQIHTPGIYVHRIF
                     QGEKYEKRIEQRTVRKK"
     gene            complement(32901..34754)
                     /gene="yidC"
                     /locus_tag="PKU82_00820"
     CDS             complement(32901..34754)
                     /gene="yidC"
                     /locus_tag="PKU82_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOZ81444.1"
                     /translation="MDRNSILGVVLIMAVLIGFSIFNQPSKEEQEAAKRKQDSIALVE
                     KKHQDSLKLITKPVAALKADTVAVADTLNNDSLSQTKLTDNFGAFALAAQGESKSITL
                     ESDLLKLKLNSKGGKIDYVELKKYKTGEGKPLVLNNGDSSQFSLSFFSENKMISTGDL
                     YFDLPAKGITVTGKDSQSVSIKLNAGEGRYIEYVYSLKGDDYRVGFKINFVNMGSLVA
                     SNAGYMELNWNQKLLQQEKSHKNESAVSTIYYRYTDEEVDHLSETKDDGTELKTKVQW
                     VGFKQQYFSSVLIADQAFESPTRINSTVINDEGAVRMMHANFTIPFSHQNNESTGMSF
                     YYGPNHYQTLNKYHIGMEKLVPLGWGIFGWVNRFAVIPVFNFLNSFNINYGIIILLLT
                     ILIKVVLLPLTYKSYISTAKMKVLQPEMSELQAKFKADPMKLQQETMALYRKAGVSPL
                     GGCLPMVLQMPILIAMFRFFPASIELRQEGFLWAKDLSTYDSILDLPFNIPFYGDHVS
                     LFTILMTVSTLIYTRMNMQMTAATNPSMKYVMYLMPIMFLGIFNNYSSGLSYYYFLAN
                     MITFGQQYLFKSFVDEKAIHAKIQENKKKPVKKSKFQERLEKMAKERGVRK"
     gene            34896..36245
                     /locus_tag="PKU82_00825"
     CDS             34896..36245
                     /locus_tag="PKU82_00825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOZ81445.1"
                     /translation="MSLPKEFLESLPLSESDKELFVQSLQKPVPVSVRINPQKHTTHF
                     SGSSVPWCRQGYFLEQRPVFTLDPLFHAGAYYVQEASSMFLEQVIFQLSLNEKPINVL
                     DACAAPGGKTTHLLSLLHQESLVVANEVIASRTPVLVENAIRWGYSNLIITRGDAVLF
                     KRLENIFDVIVCDAPCSGEGLFRKDEAAISEWSLQNVEHCALRQQRIVNDLWDALKPG
                     GYFIYSTCTSNLQENETNVQQFINKHNAECVKLKIKDFPGVEEFQRQDAVMYRFFPHK
                     TGTEIFSLAVLRKPDLNDLNSERVVAKNLKLADIVQADFAKKHIDKGEEMFYFIHDNN
                     IRFMPICLKNALGQLLNMPVIHAGTAAFEVKGKNLLPSFELALSTALNQENFNKAEVD
                     KSTMLKLLKGETRLMDSLPIGQHLITYQNIPLMFLKKIAGRVNVNYPRSWFIRMNIE"
     gene            36317..38425
                     /locus_tag="PKU82_00830"
     CDS             36317..38425
                     /locus_tag="PKU82_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81446.1"
                     /translation="MKKKATVTKQESQAKVQSGINLTQLKKYLGLLLAAIAFLLYANT
                     LNHDYTVDDGTVMQNNKIVKKGVSAIPEIFTTAYRKGFWDRNESLYRPLSVAMFAVEW
                     QIAPENPSIGHWVNVLLYVLTAWVLFRFLVNLFKDDWLFAFIATLIYVIHPIHTEVVA
                     NIKSRDEILCFLFLILSFNSFLKFLDRAKPFYALGVLFYFILSLLSKESSVTAIAIFP
                     LIAYFFRQKTIGNSIKLSWSPAIAIVAYLSLRFVVLKGISNFNEILPINNSIVNAQNY
                     IQQFATAVMIAGKYLYLLIIPYPLSFDYSYNTFPVVNFSEIKALLSLTVYVFLLIMAI
                     KGFKSRSRFSFGIFYFLLTMSIVSNVFFLIESVMAERFTYLPSLGICIALTSVLFTLF
                     KVSNVVREKIDINKIIATNRIFFGLILIVFVVYSGMTVARNAKWKNNLTLLSTDVKTC
                     PESARIRYAYGSAILIEQALKEKDYSKKMNLLDKSIEQLEKGVAILPTYSEAYYHLGL
                     AYKERENYAKAIESFEYAAKSKTFTDPQFFISWGVACGKAKRYQESIKALNHAIDLDP
                     AAKEAYLNLGVFYDEMQESDKAETSLMKAIELDSLSDGAYYNLGNVFAHRNNFVKAIQ
                     NYKKALEINPTNEDAVNNTGNSYAAMQDYENAIIYFREAIELNPSNARALNNLGVTLV
                     LTGKKDEGEQYIAKAKAIGG"
     gene            complement(38591..39898)
                     /locus_tag="PKU82_00835"
     CDS             complement(38591..39898)
                     /locus_tag="PKU82_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81447.1"
                     /translation="MPKGTRKTKFVKHTLLTFIYFALTSNSFGCSGYKITLGNKTILG
                     SNEDAWRLTPQIWFEKGKGNSQYGAAFTGSRYDGENGYAPQSGMNEMGLSFERLASYH
                     PIQEKFTNRKAITNPTKYLKDILHTCKSVEEVKEFINKYDYSYFIEDVFFYVDKSGKY
                     LIVEPYTLTIGNEPTFVISNFCPSITSEQTANKLTRYRNGVAFLKNGFDTTIAFCTAL
                     SDTMHVCRKKIGDGTLLSSIWDLNSGTVNLYFYHNYNATVQFKLSDELRKGDHIIAIE
                     TLFPHSAEFEKLRNFKTPKDSNFMGLFIVASAGFFLLTSIFFLTQFFRRKESNKYTYI
                     QLLMFPIGLIMFYYMCVLCGSVNVFYFPAPYKDPANIFVSMTSYIPFLILLLILPFSV
                     VNLRILKDNSWSFVAKGLFTFNNIIYFVLIGLFTYWRFYSIFN"
     gene            40836..41396
                     /locus_tag="PKU82_00840"
     CDS             40836..41396
                     /locus_tag="PKU82_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ81448.1"
                     /translation="MQTQQTSSTPKVIIGMDIHKKSWSVSIRTDICDHRTLNMPADAD
                     GLYDYISSNFPGHEVSIAYEAGCCGFSAARHFIGMGWKVLVVNPADVPRMDKQNYQKT
                     DKIDSRNLCKQLYNNNLRGIYIPTSEEEELRSLVRHRNQIVKQLRKVKTHIKSMLLFH
                     GITIPIDFDNNNWSKAFIKWLKNLNW"
     gene            41412..41924
                     /locus_tag="PKU82_00845"
     CDS             41412..41924
                     /locus_tag="PKU82_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ81449.1"
                     /translation="MSLDSKLRSLAFHHQEYLNIGTTLRAYCKKHYTRDYNLLRSIPG
                     IGGFLASAVIAECGDLRRFANEKQFSSYIGVVPGIYSSGGNETMLGVTPRSKSLLRTY
                     IIESAWVALRYDQEIQAYYRKHASKNPKSVIVKIAHKMVKRMLSVIKNGQPYRVNNTI
                     NKLKEEKEKQ"
     gene            complement(42288..43220)
                     /gene="arsM"
                     /locus_tag="PKU82_00850"
     CDS             complement(42288..43220)
                     /gene="arsM"
                     /locus_tag="PKU82_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOZ81450.1"
                     /translation="MNTEVIKEEIKKSYGDVAKGNIQVGCCSTETGCCTTSEFSNSMS
                     EDYSNVEGYVKSADLALGCGLPTEIANIHEGDTVIDLGSGAGNDAFIARRIVGETGKV
                     IGIDMTPEMVIRALQNTQKLGYKNVEFVLGEIENMKEISNNVADVVISNCVMNLVPNK
                     EKAFNEVNRVLKVGGHFSISDIVFQGAIPQGVLEASEMYAGCVAGASEKGQYLGIIKK
                     AGFKNISIKKERLLELPDELLLKYLNQQELDDYKKSGSGIYSITVYADKLEEGTCCDT
                     TENGCCGSETKTEKTTEKTACCGTTSNGNSSCCG"
     gene            complement(43294..43842)
                     /locus_tag="PKU82_00855"
     CDS             complement(43294..43842)
                     /locus_tag="PKU82_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ81451.1"
                     /translation="MTTDVTNIWTNFNKELKGFIYNKTRNSADTDDILQEVFVKIIRN
                     IEKVNQSENLRQYLYGIVRNTLNDYFKNQKTFSSDTEIQEQFSETETNSLNETIAECC
                     IKPFINKLPDNYKQALLITEFQDISQKELAKKLNISYSGAKSRVQRGKDKLKELILNC
                     CAYQNDKYGNLIDTEDKSCGCS"
BASE COUNT        13980 a         7455 c         8296 g        14244 t
ORIGIN      
        1 gtctaacatt aacctgcatg tcggtaaggg agaagttttt ggtgtgttcg gaccaaatgg
       61 tgcaggtaaa acaacattgt tatcattggt cacaggtatt ttaacatgta ggttgggggt
      121 gatttctatt ttagggaaag atgcatctac ccaacgaaaa aatgttaatc agataatagg
      181 atatgtgcca caagattttg cattctatga agagttgagc gctatagaga atctgatttt
      241 ttttggtgcc atgtatggat tgaaggaaca agctttggac aacagagcga aagaattgct
      301 tgaggaattt ggattaatga atgttgcaga aaggtatgtc aaagaatttt caggcggaat
      361 gaaacgtaga ttaaacattg caattggttt gttacataaa ccacaattgc tgattcttga
      421 tgagccaact gtaggagtgg atgttcagag cagattggct ataatgagtt atctgaaaaa
      481 aataaattct gagggcacaa caataattta tacttcacat ttgcttaaag aggcagaaga
      541 attttgttct cgtattgcat taattgacaa aggaaaagtt atagcaattg atacaatagc
      601 aaatttgctg atacagaatc aatgtaagtc acttgaagaa gtgtttatta aattaacagg
      661 taataattac agagactaat gagcccatta gtagcatcaa ttattaaaga gtttaaagtc
      721 ctttcgagag ataagaccgg actgcttttg atgtttttaa tgcctcttgt acttgcatta
      781 cttatgactg ttttacaaaa cagtgcattt aagattgtca acgaaaacaa agttgattta
      841 atgttaatta ataatgattc taatagtctg ttttttcaga atttttgtaa aggcattgag
      901 caaaccggca tgtttgattt gaaaattaat actaaagaga tatcaagcaa aaagttatca
      961 gaagataaag agtcaattgt agtagttact atacccaaag ggtatgctta tacacttgat
     1021 caggaagtaa aagcaaaggc agaacagtca ttgcaaatgt ttggtatgct tgagtcagtt
     1081 tcaacaaaag gaataatagg agtaaaaaat atagaactga gttttgatcc ggtacttcag
     1141 gagtcatatc gtttttcaat taagtcaacg gttttatctg tagtcatggc tattcagaat
     1201 gaaatgttat tgaaacaact tttttatgat gtcaatcaat cagaacctga ttctgctgca
     1261 ttgagtaaga tattaccaaa gccattgttg ctaagcgagt cattaattaa agaaaatagt
     1321 gttgcaaacc caaatgcaac acaacataac atacctgcat ggacagtatt tgccatgttt
     1381 tttactgtga tatcgttagg tggaaacatt gtcagagaaa aaagaagcgg aagtttttta
     1441 cgaataaaaa ctttgcccat atcaattacc caaatgttga ttgctaaaca agtggtcttt
     1501 tggattgtga cagttttaca aacgttggtc ttgttttcga taggaattct aatatttcct
     1561 atgttgcaat tgccgccatt ggtaatttca tcagggttat tgccattgtt tttggctaca
     1621 ttatgttgtg ggttgtgtgc agtaagcttt gccttgtgta ttggtgttta ttctgacacg
     1681 gttgagcagg ctaatggctt tggtgctgct tctgttgtta tactggcagc agtaggaggt
     1741 atttttgttc cattatttgc aatgcctgaa gtttttaagt taattgctaa tgtttcacca
     1801 ttcagttggg caatgaaagc ttactattca gcaatattag aaatatcacc aattcataag
     1861 ctactgctaa atgttttacc tttgcttttg ctgacagcaa tccttcagct tattgccttt
     1921 atcggattaa aacggaaaaa tttattatga caaaagaaga aattaagcca gagttaaaga
     1981 aacatattat taaatattta aacctgcttg acttaacacc agaaagtata aaagatgacc
     2041 aacctctgtt tggagaagga ggattagggt tagattcaat agattccatt gaacttattg
     2101 ttcttttaga gcgtgagtat ggtataaaaa ttactgaccc caaagaagga agaaaaattt
     2161 tgattgatgt caatacaatt gctgattata ttattcagca taagtctgca aaataatttt
     2221 ttgtattgtg gagaatgttt gcataacagg agttggtttg gttacagctg tcggaaacaa
     2281 tgcaaatgaa accatccttg ctattcaaag tggaaaaaca ggaataggta ttccaaaata
     2341 tgtaaaaggc agatatgctt cgatgatacc atttggagaa gtaaaattgt ctgacatgca
     2401 gttagccgat gaattatcaa ttagaaataa agttgtcagt cgcacgcaat tactggccca
     2461 taaagtattt aatgaggcac taaataatag tgcattacat atagatgatt tgaaaaatac
     2521 ggaaacgtca atagttgttt caagtactgt tggtggcatg taccttactg atgaattata
     2581 tgcagattca aataatcttt ctaaaggttc tgattatctt tcattgtacg attgtgcttc
     2641 aattggaatt tatttgcagc aacttaccgg agcaaaaggt gtgataaata ctattaacac
     2701 agcttgttcg tccggggcaa atgctttgat atatggaatg aggctattaa aaaataaacg
     2761 tgctaaaaga gttattgttg gtggtgtaga ttgtctttct aagtttacaa taaatggatt
     2821 taatgcactg aatatattgt caacaagaag ttgcagacca tttgataata atcgtgatgg
     2881 attaaatctt ggtgagggtg caggttttct tatactcgag tcgtcagata cagcgaaaga
     2941 agaaaaagta cttgcttata tcaaaggtta tgctaattca aatgatgctt ttcatccttc
     3001 agctttgagt gatgaaggtt ttggcccaat ggcagcaatg cgtggtgcgt tggaatctgc
     3061 gggattgatg cctaaagaaa ttaacagtgt aattgcccac ggcacaggta cacccaataa
     3121 tgacagagta gaaggattat ctataaaaag aatttttgga gaatcactgc caccggtatg
     3181 ttctttaaaa tctattttag gacatacttt gggtgctgca tcagcagtag aaagtgtgct
     3241 ggctgtaatt ggagtaagag atggccatgt ttatccaagc agaaatttcg aaactcctga
     3301 tgaggaaacc ggtcttgtac ctaacttgaa tttcaagaaa gaaaagataa agcatagtct
     3361 tcttaattca tttggatttg gaggtaattg tagctcatta attttttcct ctgtaaattg
     3421 aaaatagaac agtttaaaac agttaaatta aatggagaaa aattttgttg attatgttaa
     3481 aatttgttgc cgttcaggag caggaggtcc gggctatgta cattttcaca gagataaaaa
     3541 gaccgcaaaa ggtggacctg acgggggcga tggtggacga ggaggacata ttattgtgcg
     3601 gggtaacaaa caactttgga ctttattgca tttgaaatat cgtaaacata tacttgcaga
     3661 tgcaggacaa aagggaggta atgcattacg tacaggtgct gatggtaaag atgagattct
     3721 tgaagtacca ataggaacca ttgcaagaga ttttgaaacc aataaatttt tgtttgaaat
     3781 agaagaagat gggcaggaag tgatattggc aagaggaggg agaggaggca agggaaatgc
     3841 ttttttcaaa acatctacat tacaaactcc acgtcatgcg caacccggtg gcgaaggtat
     3901 tgaagaatgg aaaattttgg agttaaaact tatggcagat gttgggttgg ttggttttcc
     3961 aaatgcagga aaatctacac tactttctgt tgtgtctgct gccaaaccag aaattgcaga
     4021 ctatccattt acaaccttag ttcctaatct gggcatggtc tattatcatg atgacaggtc
     4081 gtttgtgatg gctgacattc caggaattat tgaaggtgca cataccggaa aagggttggg
     4141 gttaagattt ttaagacata ttgagcgtaa ttcaatactg ttatttttga ttgctgcaga
     4201 tgcaaaaagt attaaagaag agtataaaat acttaagaac gaactacgtc agcataatcc
     4261 tgaattgttg gataaaagaa gggttattgc catcacaaaa tcggatatgt tggatgagga
     4321 gttgttgaaa gaattgaaaa aagaagtacc taatgacaaa aatcccggag gaggcaaagt
     4381 gcctgtagtt tttatttcgt ctgttgcgaa ttttaatatt gataaactta aagatgtttt
     4441 gtggaaagaa cttaattcat gattgagcag ataaaagaat ttgatacaaa tttattctta
     4501 tggctgaact caatacatag tccggccatg gattcttttt tctttgctat taccaatcgc
     4561 tttgtatgga ttccggttta tctgatttta ctttttttgc taataaagaa gaaaaaaaat
     4621 aaaacatgga gcatactgtt gtttattgtt atattaatta tctgcagtga tcagctttct
     4681 gttttgataa aagaaaatgt aatgcgttat agaccttgtc ataatctgaa tattcagggt
     4741 gtagtacata cagttaataa taaatgtgga ggcaaatatg gttttgtatc atctcatgca
     4801 acaaatacaa tggctttgtt tgcgtttctt gtattgtcag tttttaaaaa agaaagaata
     4861 gttattgctc tattggcaat ctatgtctta ttagtttcat atagcagagt atatttaggg
     4921 gtgcattatc cgtttgatat tttaggtgga tggattacag gtttggcagt agcattagtt
     4981 ttgtattatt ttgtatataa taaattattc aatcagcctt aatataggca tatagattat
     5041 gcttttagtg atttaatatg aggacaattt taacaatagg ttttggtagt tttataggcg
     5101 gcatgattcg atacatgctc tcacttttta ttcaatccaa gacagtttca gaatttccgg
     5161 tcggcacctt gcttgtaaat attatcgggt gctttttaat tggcgttgtg tatagctttg
     5221 ccgaacaagg taaaatggca gtagagtggc gcttatttat tgctacagga atacttggtg
     5281 ggtttacaac gttttcagct ttttcaatgg agtcgtttat gatgttgcgt aatggttatt
     5341 atggaatgat gactgtatat gtttttctaa gtttggctat tggtatatct gctacaatag
     5401 gtggtttttt attggtaaga actcttaatt gatagttaag gaataagtgt aatttttgat
     5461 atttgatgct tataatggtt gtgttttatt ttgcataggc ctgccaacag gcaactatca
     5521 acgtatataa gccaattcca agtaatgcca tgcctgtaat cctattgagc caggtgagta
     5581 aatttattgt caatatagat tttaatttat gtgcaatgta ggctttaaat agatcaaaag
     5641 agaagagtgt gattaaaata gaaccgtaaa acaacaaaag gtgatgaatg gtgtagtgct
     5701 cttttacaga aaccgttccc gaaacaccaa tccagaaaaa tagcacagaa ggattcatgg
     5761 aattaatcat aaatcctttt aaaatataaa gactcttgct atctgttttt tcgtttactg
     5821 ttatagcttc agcaggaata ctatttcttt taagtattaa agctattcca aaaccgataa
     5881 gcaaaattcc tgaaattatt cctgctataa atctgtgatt gttgagtagt agaataaaat
     5941 tacttccaaa gtaggctatg aaaataaaaa gtgtatcact aatgagtaca cctattgcca
     6001 ctctacttgc atagctaaag cctttgcgta agctgatgtt tatgatgaga aaaaaaaccg
     6061 gtccggcaag aattgccaga aagcaaccta acagaaatcc acttattaca ggctcaatca
     6121 aaattgtagt ttataaaaaa ttaaactgct tgatttatgt tcttatgttg tgatgcaaca
     6181 aacttttccc aatcctcaaa aactttattt ttcattactc tgggaaattt attttgtgca
     6241 ccaattttgc cttgtagctc catccatttg taaaaccaag atgttggaat tatttcaaca
     6301 aaaatttctt tcaatgcact tcttctctca accctataat catcattaag aattttcagt
     6361 ttttcgtcaa taagcttttt cagattctct gagtcattat aagtatcaca accaatatac
     6421 catttgtgtg cgaataaaga ttgatagggg atacctgcaa caccaaattc agggatatca
     6481 atattcaatt catcagaaac aagttcaatt gctctgttca tgttgtcaac agacaggtgt
     6541 tcaccacata aactcagata gtgttttgtt ctaccgataa tctgaatttc acatttttct
     6601 aaattagtaa atgtaattac atcaccaatt aaataacgcc atgcaccgga acaagttgtt
     6661 aataatatcg cataaggttc attggcctta acttcgttta gtaataaagt tttcggtttg
     6721 tgttttaatt caccatcagc atcaaaatta tcttcgttaa atggtatgaa ctcataaaat
     6781 attccattgt taacgagaag tctcatacct cttgtgtttg gctcatcctg ataggcaata
     6841 aatccttctg atgctaaata ggtttctatg taataaattt gtttccctaa gagtttgcta
     6901 aatccttttt tataaggctc gaaggagaca ccaccgtgaa cataaatttc aaggttgggc
     6961 cacagttcgt gaatattttt aagcttgtaa tggtcaagta ttttttcaat caaaagttga
     7021 acccaggcag gaacacctac aataatacct atgtcccaat cttttgcctt gtgcgttatt
     7081 tcatcaagtt ttgcatgcca atctttttct ctggatattt ttctgccagg cttataaaaa
     7141 tgttgaaacc aaaatggaat ctgacttgct gtgattccac taagatcgcc ttcaaaataa
     7201 ctgccttgct gatgtaagtg tgtactaccg cctaacatta atataccttt ttcaaaagtt
     7261 gagaaaggca ggtcgtattt agataaagaa agaatctgtt ttaagctggc tttttgaatg
     7321 gctttcgaca tatcctttgt tacgggaata tgtttgcttg atgaacccga agtgccggaa
     7381 cttaaagcaa agtaacgaac cttgccgggc caacaaacat tttcctgatc atgtaaactt
     7441 ttattccacc aatttgaaaa aattgaattg tagtcatgaa ctggaactgc tgccctgaat
     7501 ttagttacaa aatttttatt ccccagcata ccttgaaagt tgtaagcttc accaaacatg
     7561 gtaaactggg cttttcttat caactttttc agtacacgtt tctgagaaat aaaaggagaa
     7621 aagggtacaa tctcaatttt tcctctgaga tcaatacttg ccttaattat tctgcctatc
     7681 aaagtcatgc taaaatgttt gcaaggctaa aaataggaaa tactttgaaa tgcaataaaa
     7741 aaacccatga attgacatgg gtttttatta aattgtactt attcttagtt gaccaagaat
     7801 tttctgcctt gggttacctg attgtcgtta ataatctgga agtaatataa tccctttgca
     7861 aggtttatag gtaggcgaac tgaattcact cccgttgaaa gcgaaacttc ttctttaaaa
     7921 attaaagctc cttttaagtc gaataaagtt atttctgctt ttatattatc gggacttgaa
     7981 acgatagagt taacaatatc ttcaacttgg tttacagaca ataatttaaa ctcatcattt
     8041 tgacatgatg gtgcataaat tgtttttaaa tcatgcatgg ttccattaaa atcatattgt
     8101 ctgagtctat agtatgcagc aatgccatca tcggttgaaa cttcatagtt cttagaatca
     8161 tttactgtgc cactgccttc aattttacgg aataatgtaa actcagtgcc atctgcactt
     8221 ttctcaagag tgaagaagtc attattaagt tcagaagcag ttgtccattt aatctgatga
     8281 tggttcttta cacaattagc atcaaaggaa agtagttcta caggtagtgg cgtgttttga
     8341 tttactaata cccaccaatc atttgtaacg gaaactcctg tagctgttac tgttcttatt
     8401 ggacctgcag aggcataagc ctgagcgccc tgaaatggat tatcccagaa tccacctacg
     8461 taattataat gttgtgcttt atatggacct gctattgctg gaaattcagc aacaggagca
     8521 gagaaaactg cattgaatgt agtgcttgtg cctgattgat ttattttcca aaaacgatct
     8581 acacaatctg cgctattgtc agcaccggta gcaacggaat taacgtgacc tacgccagga
     8641 ggataaggaa ggtttgccgg gcttgtttta aatgttgatg ctgttatgaa tccagcatta
     8701 cctcctgttg aagtaacggt taaaggtatg taggttgttg ggtctaatcc aaatgggaat
     8761 atgtgtggtg tattgtcagc agcaaacatc catttgacat tacttgcgcc atcataagtt
     8821 tcggctcgta tgtatccggc taccctggtg atagaggtgt tagggctatt ttcaattata
     8881 attgttcttc ctccattggt aggatcatta tcatcatcaa gacgcaactg acctgcagtt
     8941 aattttaatt gatttctgac gttaactgtg ccattgcttg taggataaac atactctgta
     9001 tttgggtcac caccaaagtt tatttcgagc acaccatatt tttggttgcc gccaacagca
     9061 ttacccattc caattccggt aataatctga ttgtcatcac cgaaatattc tactgtactg
     9121 gacgaagaaa gtagaaatcc catattgccg cttgaattga tagcagcaga actaaatcct
     9181 gaatagaatc cattggtatg ttgaatacgt attcttgatc cactagcacc ttgcacggta
     9241 ctttttagaa aatttcctgt agtataaaca gagctattat ttcttaatgt aaaagtagca
     9301 cctgatgaaa cataaagcat cggtaacgtt tgtccggcaa attcttcaac ataagaagtc
     9361 aactgcacat ctccggaaat tacttctaaa gttgtaccat tgacaacagt aagaacagtg
     9421 tgatctacag tatggccatt taaacgagta aataatgtag ttcctgtacg tgcaaaatct
     9481 aatgcaccgc gtgaagcaat tgttcctgca ccattgcgtt taattgttcc ggaagggcca
     9541 agaagaacgt tagggctttt aatcgttaaa ctctgtacag ttgggtttaa agtattgtta
     9601 acaaaattga ttgtgccacc ggaatgtaca aaatcacctg tattatcgtc atcactgtta
     9661 attgtaactg tagtcgtaat aggagggata gttgcattgt ttgtatagaa aaaattgccg
     9721 tttgtcatat taaatccacc taaaacatta atgataatag gattataagc agagttgctt
     9781 ttcaaactta atgtgcctcc tgaaataatc aggtctccgt ttattgttcc tgctaaatca
     9841 cctctgccgg cagaaaaatt atttattcca ttgttacagg ttaaattacc tccgacagtt
     9901 atattcacat tatgtgtggt tgtaataaca gcactaggat aattattaaa actgttattc
     9961 ccgttgtcaa atgttaaatt tgctccaatg ctgattaatt cgttaccttg agttacactg
    10021 gaatagaaag tgccgttagc acctccgaaa tacatatctc ctgtaacggt aagttttaat
    10081 ggagaagtgt tactataaac cggatgtagg taggggataa aacttactaa gtttgtaaca
    10141 gcagtataat ttacattgaa atcaccattt acagtacaaa taacactgtc gtttgcagca
    10201 ttgtattgaa tgtaattgcc tccagaaaag ctaaatcctc caagagtaaa aatacttttt
    10261 ccggctgtaa aaatgttggt tgcatgtgtg cctttaaaga atcctcctga ttggtttaag
    10321 gttccatcaa cggtaaatgt attacgacct gttccgtcct gaataccata aaatgcacca
    10381 gcactttgat taaaattacc tgcaacataa aattcaaggt ttccattgtt gaggtatgtt
    10441 ccacaaactc cctgattagc tgctgttccg gaattcatca gattaaaatc gcctagtacc
    10501 ctaataaatg tattgcctgc accggaatta cctctaaaaa agtttgccgg gtttgtagaa
    10561 ttaaaaataa aacttcttgt aacggcaatt gagtcaattg cattagaact tgcattggct
    10621 tgcacattca ttgagccacc attcagaaat aaatttgagc cgatttcaat attaactatc
    10681 ccaatgttgt tggcaacaac aaaattaact gatgcagatg atgagtttaa gtcatgacca
    10741 acaataagtt tcgttctggc aacatttccg ggagaaagca gtatggtagt attggtaact
    10801 ccagaaagat tgaagtcgtt caaaacattt actgtggcat agccagttgg taaaggatac
    10861 agaggattgt ttgaagcatc taaaagatca tttcctccca aaaaaatatt tttaagtccg
    10921 gcagaaatat ttaaatcacc tgtggtataa tttaagaatc catttccgga atcaacaaaa
    10981 cgaacataat ttgggttatt ggcaattttt gtattgccac ctacggttaa tgtaagtggt
    11041 gcatttactt gtttgcaaaa atcaaattta ttacctgagc cctgaatgag taaatctccc
    11101 ataatattag ctgtcacact agcacttctg attgggaatg ttgagtcaaa aacagcaata
    11161 aaatcgtgtc tggtaataaa attaccattg acagtcagat tcagggttcc taaagacata
    11221 ttcatgataa tagttggctg gtttactaaa acactactat cagtaaaatt ccccattgtt
    11281 aaagtaaggt ttctattatt accttcttgg aaaataattg cagaattacc tctgagtgta
    11341 acatcattca atgttaatac tgttgttgca ccgctaccat catccattct taaaactcca
    11401 tcagttacta agaggctgcc tttaattctg ttaggtgaaa actgcccgcg ttgttcccat
    11461 gttgtaccgg taacagaagt aactaagttt ccaatatcac ttgttacacg agtagggcca
    11521 gccaatggca aggcagtact tgcccatttg cgaattatta aagtgcttgt agggctgaaa
    11581 ttttctacac cattttcaaa aacgttttca tcaactgcga tattaatttt tgggttatga
    11641 tcataaaccg caccatttcc taaaacaaaa tttccgggta cagtcatatt aattctaccg
    11701 ttgttaatca atgttccact cacaactaag tcaccggcaa atactaattg tgcaacagca
    11761 ttattcgcaa tagtcaaatt accaccagag ttaatagtca ctgatcctaa tacatctgta
    11821 ttgaatccat ttatagttac agtagttcct gtattgataa caactgtatc acctgttgtt
    11881 ggatataatc ctgcttgatt ccatatcaat gtgttaaaat ttccggaggc cacagattga
    11941 aatatcaatg cttcagaacg gtaagaactg agaacaaaga ttaaagtgac cataaaaaag
    12001 gaaatgattt tttggaaatc tttcatgttt ttattgattt aatagttttg gatgacatct
    12061 aatgagatgc ctctgtaagt ttaatcggta aatttactaa aaggttaaga ggaaaagcaa
    12121 ttttcttacc actttttttt cacgaatcat atctacatca agaagttggt aatatgggaa
    12181 ctgcgctccg ataagaattt ttaataaatt aattaatttt catgaattaa aactttaata
    12241 aaaatatatt aaacaattga caattaatat aatatagtaa tatttaatta tgccgcaaaa
    12301 tcggttgaaa tggtattcat tttcatttat ttatacataa ttgttatgaa aaaatgtaaa
    12361 aaaaatatta acatgaaaga agtcgtaaga tcatttacaa gtaaaacagc ctatttcccg
    12421 acaaaatctt tcaggtagaa aggttcaaaa taggctaggt tttcaaaagt ctgctgagta
    12481 aattttttaa aagcaagtgt cactaagccc attgcagaca ttttaaattc atcgaaaatg
    12541 acgcaattgg aattgatttt tattgaatcg ggaagttttg ctgcaccgtt accaaaaaag
    12601 caaatcttgt tttttactaa tttatcatga tgaaattgtt catcaataat aactgcattt
    12661 tctttttcga taaaatcgag ttgtgggttt aatagtgctg taaaaacttc cattcttcgt
    12721 gcgtcaatca ttgggcataa tagctgacct ttgcattgtt tgtttgcaat gtttgccatt
    12781 gactgtaaag tagcaacaga aataagtggt atttttaatg caaaacaaag tcctttggct
    12841 gtggctacgc caatgcgcaa accggtataa gacccggggc cgcaactgac aacaacagca
    12901 tcaatgtttt tataagtcag tcggtttctt tttaacaatt catcaatgaa aacagcaagt
    12961 aaagatgcat gcgatttgct gttatgcgtt tcttcacaat ctatgattgt ttcattgtcg
    13021 gaaatacaaa cagaacaagt gtcagtagaa gtttctaaat ggagtagaag agccactaaa
    13081 attatttttg ttcttcttta aaaagctgat tttgtggaac aactttaatt ttatcaccat
    13141 caactaattt ttttgataca gcctgatagg ggtcggtaat tacttcgtca ccaactttta
    13201 ttccttctag aatttcaata aaattattgt cctgaatacc tgtttttaca acatgcattt
    13261 ttgcaatgcc attgtcattt acaaagacta tttctttaac aggtgtatca tttgtgttac
    13321 tgcttacaac tactttgtca tcaggtggag tatcgtcatt ttttctgaca acagctttgt
    13381 cagatgttct gatggtggta tcacgtgttg taactgcctt gattggaaca gaaacaacat
    13441 tacttacttt tttagtttga atttcaactg ttgctgacat gccaggtcta aatggagaat
    13501 attccggatg gtcagaagga atcaagtcag aataagattc acgcaagatt ctgattttaa
    13561 ctgcaaagtt tgtgacttgc tctgttgatg tcaatgactc atttggtgaa ttggcaatct
    13621 ctgttacaat gcctttgaat tttcgtgggc tataggcgtc aatttcaact aatgtagtat
    13681 catttaaatg tattctaatg atatcgtttt ctcctacatc ggcttttact tccatttcct
    13741 gcaagttaga aattcttaga atttctgtgc catcagtaaa gcttgttccg gcaatacgct
    13801 cacctttttt cttgcttatt ttagaaatgg tgccatttac cggtgcaaaa atttcagtct
    13861 tgtttaaatt ttctttggct tcgcgtacag ttgcacttcc actgttgatg ttatattgtg
    13921 ctcctgaaac gccttgtttg gatgcatcta cttctgcttt tgcaacttca taggcggatt
    13981 ttatagcttc gaaatcggat gcgcttatga ctttttcttc aaaaagtttt ttatttcttg
    14041 tgtaggcaat ttcagctttt accaactgac tttctgcttg tgatagtcgt gacttgctgc
    14101 tttcataatt tgcttttgta gaattcagcc cggcaacagc tctgtctaaa gatgatatgt
    14161 atatttcagg attgattttg cataacaact gacctttctt tactacatca ccttctttaa
    14221 caagcatctc aacaatctcg cctgaaacat cagagcttat ttttacttct acttcaggct
    14281 gtacttttcc acttgcagat acagactctg tgatggtttg taatgatgcc ttttcagtgc
    14341 ttacattggt gacattttct tcgccaagcc atcccgcttt tttggcaatt accaaagcca
    14401 caataacgac tccggcaatg atgataatat ttcttaatgt attatttttc ttactcatac
    14461 aatttactta aaatgacaat ggttttccca gataatagtc aagtactttt atttttaaaa
    14521 tcaaatcgaa ttttgattga atcaaatctg attgtgattt ggtgtaatta tttctggcat
    14581 ttacgtagtc aatagaattt aatagaccag cattgtactt tttttctgta tataaaaatg
    14641 ctttttcctg tgctgtaaca cttttgtttg cggcagcata tttgttgagt gcagccaatg
    14701 catcggcatg agcctgctga acatttttaa acacagtgtt tttagtttgc aaggcattga
    14761 ggcgtgcatt ttcaaaactc aattttgatc ttttaatgct gctgtttgtt gcccatccat
    14821 taaataaggg gatggacaag ttgaatccga tagcttttcc gaaattattg ttcagctgat
    14881 ctttaacagg agtatcaata tatttataat tgaaaacagg ttgcagcact tgctctcctg
    14941 atgatgtaac cgcgcctgac ggtacatatc ctgaaaaaac aggattgcca tcgagagatg
    15001 ttgcctgatt ggaataattg gtagataaag agccaaatgc agataacctt ggataacgag
    15061 cagcacgtgc aatacctaaa gattcttttg cactttttaa tctaagttca gaagctttga
    15121 cctccggcag attttttaaa gcttcattgt aaacagattc tgatgataat gaaagcgatt
    15181 cctgagatgg gatttccagt gcaggagttt ctattgcaat gtcattcgca ccttcaagtt
    15241 gcattaattg aattagcgtg agttttgatg aggtgagcag attttccgca gaagctttat
    15301 tgaattcttc agttgcaaga agtgcatcgg catcaagcaa tgagccttca gctaacaaat
    15361 cattgtcaac taaaattttt gctctgttac gctctttagt aactgcttct attctgtcgt
    15421 tagttgcttt taattgctca ttactgtaaa gtatctgaag ataggctgct gcaaccgata
    15481 atgcaatatc attctgcact tttcgtacat ccagtttact tacatcaaaa tcaatggaac
    15541 ttttacgtaa attatgttgc aattccagtc cttgaaatat tggaagattt ccatttaaag
    15601 atgcaccacc tgactgaaaa gtttgatcaa catattgata acttgtaggg tcaaggcttc
    15661 ttccgtagtt gtaatttaaa gaagaactgg catttatact gggaaaaagg ctgccgacac
    15721 tttgctgata agtagctgct gcaatttttg aagcaatgat tgattgctgc acactaatat
    15781 tattgttcaa tgcatagtca atacagtctt taagcgacca gattttctga gcgttagcct
    15841 cactgaaaaa taatataaat aaaaaaatag atttaaaagg tttcatgtta ttccggtaac
    15901 tgcgttgcga aattaagaat tgtttgattg agagagcttc aaagctatat taattaatgg
    15961 aaatctatat ccattaacaa actttaacag ttagaaattg tgttgtcctg agaaaaattc
    16021 acatcaattt ttcagactga ttttatattc ataaaaaacg gttaaacttt cttattttcg
    16081 cacactattt tattaatcat gttcgagaat ttaagtgata agttagaaaa ggcgtttaag
    16141 aacctaaaag gtcagggaaa gataaccgaa attaacgttg ccgaaactct aaaagaggtt
    16201 agaagggcat tattagatgc tgacgtaaat tataaagtag ccaaacaatt taccgatacg
    16261 attaaagaga aggcattagg tcaaaatgta ctaacggcag tatctcccgg tcagttgttg
    16321 gttaagattg cacatgatga actggctaca ttgatgggtg gtcagatgac agatattcaa
    16381 cttaaaggaa atccatcagt cattctaatg tcagggttgc aaggttcggg taaaacaaca
    16441 ttatccggaa aacttgctaa ttttttaaag actaaaaaag gaaagacacc tttgttggtt
    16501 gcatgcgatg tttacagacc ggcagccata gatcagctta aagttttggg tgaacagata
    16561 ggtgtagaag tttattctga agtagaaaat aaaaatcctg tagaaattgc gcgtcatgca
    16621 ataaagcaag ccaagcagaa tggtaacaat gtagttgttg ttgataccgc aggtcgttta
    16681 gctattgacg agcagatgat gaatgagatt gcagcattga aaaatgcaat cgaacctgat
    16741 gaaatcttgt ttgtggttga cagtatgacc ggtcaggatg cagtgaatac agcaaaagca
    16801 tttaatgacc gattgaattt tgacggagtg gttttgacta aacttgatgg tgacacaaga
    16861 ggtggtgctg cattgtctat tcgttcagta gtaaataagc ccataaagtt tgtcggtacc
    16921 ggtgagaaga tggatgcgtt ggatgttttc catcctgagc gtatggccga ccgtatttta
    16981 ggtatgggcg acattgtgac attggttgaa aaagcacagg aacaatttga tgagaagcaa
    17041 gcagccgagc tgcaaaagaa gtttgccaag aatcaattta attttaatga tttcctttcg
    17101 caattgcagc agattaaaaa aatgggaaac attaaagacc ttgtttcaat gatacccgga
    17161 gttggtaagg cagttaaaga catggacatt gacaacaatg cttttaaaag cattgaagca
    17221 atcattcagt caatgacacc ggcagaacgt gccaatcctg atctgattaa tggtaacaga
    17281 aagaaaagaa ttgcagccgg tagcggcaca ggtattcctg aagtaaataa attgattaaa
    17341 cagtttgagg atatgcgcaa gatgatgaag atgatgacga caggtaatat gaaaaacatg
    17401 atgcgcaata tgcccggaat gagaagataa gttatcgcat taggacaata tttttagggc
    17461 atgacaataa ttgatggaaa gcaaacagca gaagaatata aatctattct aaagcaggaa
    17521 gtaaaagcac ttgttgcaac cggcaagcgt cctccggggt tagctatatt actcattggt
    17581 aataatggag caagcgaaac ttatgttgct aataaattaa agacttgtca tgatgttgga
    17641 tttaatgcat cgctttttcg ttttgaaaat acggtgagtg aaaacgaaat attgaactgt
    17701 ataaatgaac tcaattttaa ttcaagcatt gatggtgtca ttgtgcaatc accgtttcca
    17761 aatcatattt caccacagaa aataaatgaa gctattcatc ctgcaaaaga tgttgatgga
    17821 tttcatccga tgaatgcagg gaggctgcaa cagaacaaac catgttacat tgcagcaaca
    17881 cctaaaggca ttatgcagtt gattgaaaaa tataaaattg aaacacaggg gaagcattgc
    17941 gtggtagttg ggcgaagtaa tattgttgga atgccaatga gtattttgtt gagtcgaaac
    18001 agtaagcccg gaaattgtac agtaacaatt tgtcatagca agactgtaga tcttgcagca
    18061 tatacaaaac gtgctgatat tttaattgca gcaattggtt cgcctgaatt gataacagca
    18121 gatatggtga aagaaggtgc agtagtgatt gatgtaggta ttacacgtgt agcagacaaa
    18181 gcaacaaaaa gcgggtacag acttaaagga gatattaagt atgatgaagt agcccctaag
    18241 tgtagtttta tcacaccggt tccggggggt gttggtccga tgaccattat cggattattg
    18301 cagaatacct tacttgcggc aaaaaatgcc atctataatt aatctatttt gaaatgattt
    18361 ttgaacagga attagcgagc ataacaaatg aagcattaaa agtgcttttt aattttgagc
    18421 cggatgaaag tctgttgact tacacgcaaa ctcgtaagga gtttgatgga gattatacta
    18481 tagttgtttt tccttttgtc cgtcagtcga aaaaatcgcc tgaagaaaca ggaagattaa
    18541 ttggagagta tttaatcaaa catgcatctt ttattagcaa ttacaatgtc ataaaaggat
    18601 ttttgaatat tacggtaagt gatgagcagt ggcttaaaag aattgcagtt gcatttgcag
    18661 attccgaata tggttttact aaagcagata aatctaagcc tgttattgtt gagtatgcat
    18721 cgccaaacac aaacaagccc atacaccttg gacatttgcg aaatatcctt ttaggttatt
    18781 cagtttcatt gctactgaaa gcaaccggac gaaatgttaa acgagttagc atcatcaacg
    18841 acagaggtgt gcatatatgc aaatcaatgc ttgcatggaa aaaatttggt aatgatgaaa
    18901 cacctcaatc atcaggaatg aaaggtgatc atcttgtagg aaaatattat gtggagtttg
    18961 acaagcaata taaacagcag gtttcagaaa tgatttctgc aggtattgaa aaagataaag
    19021 cagaaaagaa cgcaccctta atgcaggaag taaggcagat gctgatagag tgggagcaag
    19081 gcaatgaaga agtgactgca ttatggaaga agatgaatgg ttgggtgtat gaaggttttg
    19141 atgttaccta taacagatta ggtgtagatt ttgacaagac atattatgaa tcggaaacct
    19201 atctgctggg aaagaaaata gttgaagaag gactgtcaaa aggtgtgttt tataaaaagg
    19261 ctgacaacag cgtttgggtt gatttaacga atgatggatt agacgaaaaa atagtgcagc
    19321 gctctgatgg aacttcggtt tatattacgc aggatttagg cactgcagtg ttgcgtcatg
    19381 atgagtttga tttcgattct aaaatttatg ttgtaggcaa tgaacaggac taccatttta
    19441 aagtattgtt tttaatactt aaaaaattag ggtatgaatg ggctgaaaag ctttatcatt
    19501 taagttatgc aatggttgat ttgccaagtg gtaaaatgaa atcacgtgag ggaactgttg
    19561 ttgatgcaga cgatttgatg gacgaagtaa ctgctgctgc cgctgaagaa acaagtaagt
    19621 tgggcaaggt tgaagagttt agcagtgatg aattaaagca gctctatgaa acgcttggct
    19681 ttggggcact taaatatttt attttaaaag ttgatcctga aaaaagaatg ttatttaatc
    19741 ctgctgaaag tgtagagata catggaaaca cgggtcctta tattcagtat gtgcatgcaa
    19801 gaatttgttc tgttttgaag cgggcttcac agatggaaca actgagcaac tataaaaata
    19861 ccgcaataga atcacttgtt agcatgaatg agaaggagcg tgaacttgca atcaatattc
    19921 ttcgttttaa agaaacagtt gaaactgcag cagaaaaatt ttctccggct attgttgcaa
    19981 actatgttta tgagttagca aaaaacttta atcaattcta tcacgataat tcaattattt
    20041 cagagggtga attggaaaca agccagttca gactgtgttt ggctgatgcc tgtgcaatga
    20101 caattaaaaa atcattgaac ctgcttggta ttaaggcccc ggaaagaatg taagagattg
    20161 tttgagttga attaaattgc ctttttcaac ttagcaacag catccagaac aaactgtact
    20221 tgttcatcac gactcatata ggtgttgtca agaatgattg catcatcagg ctttcgcaat
    20281 gggctttctt ctctgtgagt gtcttcatag tcgcgcaact caatattttt tcttatttca
    20341 tcaggtgtta cttttattcc tttttcaatg agctcatgat atcgtctgta aacacgtact
    20401 tcgggtgatg cagtcataaa aattttcaag tcggcattcg gaaaaacggt agtgccaata
    20461 tcacgtccgt ccattaccag atttccaaga gttgacattt gttgttgcat ggcaactaat
    20521 ttttttcgta ccgattttat tgcactcaca taactaacat tgtctgaaac atccatctgt
    20581 ctgatttctg cttcaatatt tttgccattg agatagattt ctgattgtcc ggtttgttca
    20641 tttctgataa attcaatttg aatgttttca agaattgaag aataatcagc aattacatca
    20701 gcagtcttgc tgaatggaat attgttttgc aggaaataat aagtgactgc gcggtacatt
    20761 gcacccgaat caatataatt caatttaagc cgctttgcta tggcttttgc aaccgtactt
    20821 ttcccacagg aagaataacc atcaatagca attatcataa aacttatccg gaaaactttt
    20881 caaaaataat gaaaagcaat ataacgacag tgaatattaa ctttatttca ggttagctgt
    20941 cagttgtttt tggtttgcgc ttaaaatcgt ttagatatgt tgaaatagaa aggctgttgg
    21001 ttccaccagc aagatggtaa aggcttctgc catagctgag gtgaaattta gatattttta
    21061 aaccaagtcc gaatgaaata ccaaccatgg caggtctgct ttctactttc atttcatttc
    21121 gtctctggaa attgtacgcc atgcgcaaat gaaaattttt actgataaga atctcattgc
    21181 tgatgattaa atgtcgcaat agtttattgc caaacgatgc tttttccggc tcttttactt
    21241 cacctgtcag agggtctgtt tcctgactgt tgttgtcaat gtaggaaaga tcccattttt
    21301 caatatgacg ataggttaaa gtaaaacgaa aaggagcttt ggctaatttc tgtgtaatgc
    21361 ctgcttcaat ttcaatcggc agtggctcat tgtttttctc agtatatgat tttaattgac
    21421 gaccggcatt tctgataaca agtgcagcac ttgtgagttt atcgggactg taatagttga
    21481 ctccaaggtc ggcagcaatt ccaaatgcag agtaggtgtc aatgtgactg taaataaatt
    21541 tcaatgaagc accaacaaac aacacactat caattccaac aggtcttgac atagatatgt
    21601 ttaatgcata atcgccacct gaaaaagttc ctaagtcagt tgcagtttcg tctgtgcggt
    21661 cgaacttccc atagcttaca tattgtatgc cggcagaaag tgttcctata ttttcaatgt
    21721 gatgtgcata ggcagtgtaa ccatatttta ttccggagaa atatggattg tagttgaagg
    21781 taatctgttt atccatatca acatttaata tggcaggatt gtaaaaggaa gtatttaagt
    21841 cgttgtcgcg aatggaaatg aaatttccac cgagtgctgc atgacgtgca gatgatggca
    21901 ggtcaagaaa acgaaagatt gcacttccgc ctgtctgagc tttcaaacaa acaggaagca
    21961 acagtgctgc tatcagagtt gttattctca tggaatttgg tttatgcact ctcaaacgct
    22021 aatcgccaaa aaatattttt atcaaacaaa ataaattaat aaagaggaaa atgttccatc
    22081 atgtcgttga cagtagtctt tacttcttca attacagcgc cattcgtatg attcataatt
    22141 accatatcaa tcaattctac aattcgagcc atttctgttt ctttcattcc tcgtgatgtc
    22201 attgccggtg ttcctacacg catacctgaa gttacaaaag gagattgtgt gtcgaatggc
    22261 accatgtttt tgttaacggt gatatctgca cgaacaagag cattttctgc ttgttttcct
    22321 gtaatgtttt tagaacgcag atcaatcaac attaaatgat tgtctgttcc accggaaatt
    22381 accttgtatc cctttttcac aaaggcatta gccatagttt tagcatttcg aattacctgt
    22441 aggcaatatt caaaatactc atcgcttaat gcttcaccaa atgctacagc tttagcagcg
    22501 ataacgtgtt ccaatggtcc accttgcgtg cccggaaaaa ctgcactgtc cattaaggca
    22561 gtcatcatgc gtgtttcgcc ttttggagtt ttcaatcccc atgggttttc aaaatctttt
    22621 cccatcatga ttaaaccacc acgtgggcca cgtaaagttt tatgtgttgt agtagaaata
    22681 aagtggcagt gtggcaatgg atcattaagt aatccccgtg caattagtcc tgatggatgt
    22741 gaaatatctg caagcaacaa tgcattcact ttatctgcaa tggaacgtag tgcaacataa
    22801 tcccaatcgc gagagtaggc agatgcaccg cagataatta attttggttt ctcttttaaa
    22861 gcagtttctt caactttgtt gtaatcaata gtgcctgtat tttcttcaac accataaaat
    22921 gttggacgat aaagtttgcc cgaaaaattt acaggtgaac catgcgttaa atgaccgcca
    22981 tgagaaaggt taaagccaag aattgtatca ccgggttgca ggcatgcaag cattacggct
    23041 gcattagctt gtgctccgga atgaggctgc acatttgccc atgttgcatt aaataaagtc
    23101 ttagctctgt caattgcaat ttgttcaatt tcatcaacaa cttcacagcc gccataatag
    23161 cgtttgccgg gaagtccttc tgcatatttg tttgtcagac atgagcccat ggcgtccatc
    23221 acttgttggc tgacaaaatt ttccgatgct atcaattcca aaccatgacg ttggcgtgca
    23281 agttcttctt taatcagttt aaaaacagct ttgtcttttt gcatatttgt agagtatgtt
    23341 ttctaattat tttttgtgat tatttacgca tcaatgattc aatgtcactt acttcaacag
    23401 gcattgtggc catcaggtca actggtgctt tatcggtgat gagaatgtta ttttcaatac
    23461 gtattccaat gctttcttca gggatataaa tgcccggctc acaagtaaac accattcctg
    23521 cctgcatagc ctgatattta ttgcctacat catgcacatc aagtccgagg aagtgcgatg
    23581 tgccatgcat aaaatatttt ttataagcag gccactccgg attttgattt ttaatttcgt
    23641 ccttagttat cagtttaagg ttcaccaatt cttcttccat cattttacct acttcggcat
    23701 gatacttttc tataaagttg cctgggacaa gaagtttggt tgcagctttc atcactctca
    23761 aaacagcctc atatacttct ttttgtcgtg gcgtaaattt tccgttcacc ggaatagttc
    23821 ttgttaaatc tgcagcatag tttgcatact cagccccaaa atccatcagc actaaatcgc
    23881 catctttaca aggcttgttg ttttcattat aatgcaaaac acatgcagaa gcaccggagg
    23941 caattatagg actgtaggca tgtccattgc aacgattgcg aacaaactca tgaataattt
    24001 ctgcttcaac ttcaaactcc atcacaccgg gcttcataaa ttttaaaaca cgtctgagtg
    24061 ccgattccgt atgtttaata gcttcgctta ttaactgaac ttcgatatca tgctttcgcg
    24121 aacgcagttt tgccattatc tgaccaagcc gttcaaaatt atgtgcagga aaacggtttt
    24181 tccattcacg tgcaaaacgc atatcgcgat aaggcacttc tgtagcatga cgatcgtttt
    24241 cattaatgtt gacataaaca ttctctgcat ggtgcatcag cataggaacg acagcgttaa
    24301 actcttctgt ccaatagata tgctttatac ctgatgttgc ctgtgcttct tccttagtgt
    24361 atttatgtcc ttcccagatg gcaatgcgct cattggtttt tctgacaaat aatatctctt
    24421 tgtattgcgg caatggtgag tcaggaaata ttaccaaaat actttcttcc tgatcaatac
    24481 cggtgaggta aaacaaatct gcattctgac gaaaagtgaa attctgatcg ccactacgcg
    24541 gcatttcatc attagattga aatatagcaa ttgatttagg ttttaattct gctgtaaatt
    24601 ttttcctgtt gtcaacaaat aacttgctgt tgatggaatt gtacttaata ttcataacta
    24661 actttacttt taactgttac aaataacgtg aaataatttt tacgtgcttt gtttttttta
    24721 atgttttaaa tgagcctgtg agcaaatatc gggactaaaa gaatattgac gagcaatatg
    24781 ttaagaatag aagtttttaa tagtgtgagg gaaatgccgg caaatgactg gcagaccata
    24841 aatggtgaaa atgtcatttt tacttcattt tcttatttgt cggcattaga acaaagcaag
    24901 cctgccggca tgcagtttag atatgttatt gtttatgata gtaagatgcc tgttgcgact
    24961 ttgtgttttc aattgcttga tgttgccaat aaagatttgg atggtgtatt gaatttaaaa
    25021 gacaaaggtt ggttgttgca gaatctgaat aaatcaatca acaggctgtt atttaaatgc
    25081 caaaccggaa aagtaaactt tttaatttgt tgcggaagtt tttttgtaag cggtgaatat
    25141 ggtatcaatg ttataaatag agatgccttg cctgcagtaa ttcaactttt accggaagtg
    25201 agacagaaaa ttacagatac aattagtaat gcatcggtgt gtggatggat ggtaaaggat
    25261 ttttataaca acaccaaggt tgatcaacca ttgctggaag atgatttttt tgaattgcca
    25321 atggacccag agatgatatt atacatcaga gaacagtggc attcgttcga agaatacctg
    25381 gcatcgctca caacaaaata tcgtgttaaa gccaatagtg taataagaaa gattgaagat
    25441 gttgacgttg ttgcccttaa tgcagcagaa gtactaaaat atggagatga attaataagg
    25501 ctgtatttaa atatacagca aaaggcaacc gtgcaaatgg cgagagtcaa tattttatat
    25561 tttgaaaaac tcttaaccat aatgcctgaa aaattttacg taaaggtttt ttttaaagaa
    25621 agtaaagtga tagcatttta ttgtgggttt catcatgccg gtcatcatga ggcacatttt
    25681 ataggtattg attacagttt gaataaaaca ctttctcttt atcagaatat attatatcgt
    25741 tttattgaag aagccatcag tcaacgctct tctaatcttt attatgggcg gactgctatg
    25801 gagataaaat ctactaccgg agccaaagcc tatccattac atgcttattt taatctaagt
    25861 aatactgtct tgaataaaat ggtgaagcct gttgtcaggc gatttacacc agaggagtgg
    25921 attgcccgaa atcctttcag ggagtaatta tccaaaaacc gtttgccatt tttatttgaa
    25981 ttttcacccc aaaaattaac atgttaacat aaaacagtgc cttgcagcat tggtggtgtg
    26041 atagcttgca agcatcttaa ttgaaccacc aatgattaga acaattcaaa acacaacaaa
    26101 agcagccatc atactgatta tgttcagtta tattggcaat ccatttcctg cttatgcaaa
    26161 aggaaaaaca cttaaagcaa cattaaaagg ccagaatgtc tcttgttttg gcggcagcaa
    26221 tggctctatc agtcttgata tttcaggcgg taaggcgcca tatcaaatta catggaatga
    26281 tggtacgtca ggctatgcca tgttagataa gcctgcaggc gagtattctg tttcaattac
    26341 agattctaaa ggcagtacgg tttcgcagtc tattacactt actgaacctt atgctatgac
    26401 aatttattcg caagttactt ccgaacagtg ttatgcccgc aatggtaaag caaacttatt
    26461 ggtaacaggc ggaacacaac catatcagta tgaatggtcg aatcaaaccc gtcagcaaaa
    26521 cttggaggca gttgcggcag gcgattatga aataaaagtt acagataaga atggctgtat
    26581 aaagcagtcg aacattacta ttgacagaaa caaagttctt gagttaaaca tagcaaagta
    26641 ttctccttta tgtaatgacg aagccagtgg cttaatagat gttgaagtta aaggtggatg
    26701 gtctccatat acctatgaat ggtcgaatgg tgctaaaaca gaagatcaga gtggattaaa
    26761 agcaggaaat tattctgtga tcgtaaaaga caataatggt tgcactgcaa aagcagaagt
    26821 tcaattaaaa aatccggaaa cgataaaagt tgaagcaaaa gttacagagg cggatttgaa
    26881 taaaagcaat ggtagcataa tgttacaggt ttcaggtgga acaggaaatt acacctacct
    26941 gtggtcgaat atggaaagca gtcagtcgtt gaataatgtt gaagaaggcg tttatgctgt
    27001 tagggttgat gacgcgcacc gttgtagtgc agcagcatat tttacagtga atgaaaaatc
    27061 gcctatgcag gttgaatcgg ttgtgaaaca tgaattgtgt aatggaggga aaaatggttc
    27121 aataaaactg aatgtaaaag gaggagttgc accttataaa tttcaatggt caaatggtat
    27181 gaatggagca acaataagta atcttactgc cagtgattat agtgtgctca ttaccgatgc
    27241 caataataaa gtttacagaa atacttttac tgttaagcag cctgagccaa taggaatatt
    27301 tgcaacagta cttgatgaaa gttatacagg aaaaaatgat ggcagcatta aattgcaggt
    27361 gataggagga atgcctccgt taaaatatca atggagcaat ggtcaaaccg ttgctgattt
    27421 agcaaactta tcgcagggaa catacacagt gcgaatgaaa gatggtaatg gttgtgctat
    27481 ggaacaccat gtacttgtag gaataaacta tactgatgca atgttaagaa ctgcttttgc
    27541 gaatgaagat aatgcaatga tggaaattta tcccaatcca ttcagtaaca attttacaat
    27601 gaaattcagt acaccggtta acgatataca aagcattatt gtttatacaa tggatggaaa
    27661 aattgctcag cagcttaatc ctgatttaaa acaacagcag gttaatgaaa ttgctgtcaa
    27721 tgcatctaaa ttgaaacccg gaaattattt agtaaaagta accacagaca aaaaaatctt
    27781 tacaagaatc gttacgcgaa caatgtagta tcaatacttt tcatggttgg ttgtttttca
    27841 caggcgcagt tggtggttct ctgcgcctgt aaccgttttt aagggttagg aagtaattct
    27901 taatgaagta aaaaaatatc tgaaataacc ataccggcaa aaacaatgct ggcaattcca
    27961 ttggtagtag caaaggcaat gtttactttg ctaagattat tttcagaaac caaactgtgc
    28021 tgataaataa gcagtgcaca aaatattgca agccctgacc atgcaattaa acctacatga
    28081 gcctgataaa gaaaaagagc caaaaaacct gccgataaaa aatgcaaaat ccttgaaacc
    28141 tgcaatgcct gtttttttcc catgagtgat ggaatggaat gcaatttatt ttcacgatca
    28201 aattgctcat cctgcaaagc ataaataata tcgaacccgc taacccagca aagaacagca
    28261 acaccaatta aaattggagt aatatcaaac tttcctgtaa ctgccagata agcgccaacg
    28321 ggtgccaatg caagtcctgc gcctaaaacc aagtgacaaa gtgctgtaaa tcgtttggta
    28381 aaactgtagc caagcacaac aaataatgca acgggcgaca gaaaaaagca aagtttatta
    28441 attaagtatg cagtcactgt aaaaagtaaa gcattaacaa ttacaaacca caatgcacgg
    28501 tttgctttaa tgatgccggc cggaatttcg cgcagtgctg ttcgtggatt caatgcatca
    28561 atacttctat cggcccatcg gttaaatgcc atagctgcat tacgtgcagt tatcatgcag
    28621 actaaaactt ttattgccaa cggccaaaaa acaggcagcc cgttttgtga aatagccaac
    28681 gaaaatccaa ttgcagcaaa aggaagtgca aacacagtat ggctgaattt tacaagcgat
    28741 aaatattgtg atgttttttg gagtacctgc ataatttaat caattgcaaa ggtaatcatt
    28801 ccttatggtt tacattttca aagaagctat ttgctaaatt agcccaatga attttttggc
    28861 acatctttat ctttcagaca ataatgagcc acttatggta ggtaatttta ttgctgatca
    28921 tttaaaggga aatgactgga aaagtttgcc tgaaggaata atgtcgggtg taaaagtaca
    28981 tcgttttatt gatcatttta cagatacaca tcctgttgtt gaacaaagca aggtactgtt
    29041 gcgtccttac tttcataaat atacaccggt aatcagcgat attttttacg atcattttct
    29101 ggcagcagaa tgggataaat attccaatca accaattgat gaatatgcaa gatatgttta
    29161 tcaacttatg cataagaatt atcagttttt acctgcacgt accaaagaaa tgctggttta
    29221 tatggagaac gacaactggt tgaaaaacta cgctacatta aacggaatgc atagagcgtt
    29281 gagtggtatg ggcagacgtg caaagtttga taataacatg aatcaggcag cagaattttt
    29341 gtatgataac tatgagctgt ttgaaaaaca cttcaaggag ttttttccgg agttggagag
    29401 tgcagtgaag aaggaaattt ctacacaaac ttgattttcc ttgcagcaaa aaaacacatg
    29461 cgcaacaaaa tgatgccatg tttaaaacgc gatatgttgg tgctgccgta ggtgcgttcg
    29521 cgatagcgga taggtaattc ctgtatttta agattgagtt tgtaagcacc aaaaatcaaa
    29581 tcaaaatcac caaaaggatc aaagtcgcca aagaactttc tgttggtaac caaccggtcg
    29641 taatcatctc tgaaaataac tttggtgccg caaagtgtat ctttaaaagg ttgctctaat
    29701 aaccacgtaa aagcctgact gaagaatttg ttgccgagca tatttaaaaa ccgcatggct
    29761 tctttttcca ttggataaat taaacggcag ccattaataa aatctccttt attgctggca
    29821 atggcttcat aaaatttggg caaatcttcc ggaggcatgg taaggtcggc atctaaaatc
    29881 atcaggatgt cgccttctgc cattgagaag cctttgcgta cagcatcgtt ttttcctttg
    29941 ccttcttgtt taccaacttt aattttatgt gaagcagcat atttactgga aacttcgtta
    30001 atgctgtccc aggtgtcatc ggtgctgttg ccttcaataa aaataacttc ctgccacttg
    30061 ccaaatttgg gaatacgtag caatccattt tcaatattgc cgctttcatt gcgagcaggg
    30121 ataacaatac ttactgaata tttttcgttg acatcttctg ttgcatttgc caagggctta
    30181 gcaacagagt aggtgttgat acacatcaac ctgaataaag gcagctttgc tataaactta
    30241 tttatcagtg gagataagag cggaatgttt attggcaaca acatgcgcct cgactctcga
    30301 taacaatcaa agcctgcaag aaacagaaga ttgcggacat ccatgctggt gagccaattc
    30361 aattgtgggg tcttggtttt caagccaagg ttttctgcta agcgtaaaaa tggctcccat
    30421 agatggttgt gataggatac aatgatttta gtacgctcat gacaaacttt tttcaattgt
    30481 tcaagaacag cttgtacgtc ttcaaggtag cctgtcaggt ttgaaagaat aattaaatca
    30541 tatttctctt cgagtgaaat attttctgca gccatcacct gaaaatcaat ctgtggaaat
    30601 tgtttttttg cttcggcaat cattgtaggg ctataatcaa tgccggtttt ttgggcagca
    30661 cggatgttgt ttaacaactc accggtgcca cagccaactt cgagtacaga aaatgtttca
    30721 tgagaaaaat aattgcagta aaaagttata tcgttccaat aataacgatg tttatgccgg
    30781 tcacgaattc ttttctcagc aattttttca aaatggtcaa ggtatctcat caatgcagaa
    30841 agttaatcta tcaccatttc ggggatatca ccttcaacaa taagttttcc ggcagtagct
    30901 tttttaatat cttcaacaga aactccgggg gcgcgttcaa gcaatttaaa tccttgtgga
    30961 gtaacatcaa gaactgcaag ttcagtaaca actcttttta tgcatttaat tcctgtcaga
    31021 ggcaaagtac atttaggtaa aagttttgat tcgcctgctt tgttaacatg ctgcatagct
    31081 acaataatat tttttgctga tgctactaag tccatagcgc cacccattcc tttaaccatc
    31141 ttacccggaa ttttccagtt ggcaatatct ccttcttcgc tcacttccat tgcaccgaga
    31201 atggttaaat caactttctg agcacgaatc attccaaagc tcattgccga atcaaaaatc
    31261 actgaaccgg gaagtagcgt aacggtttgt ttacctgcat taattaagtc agcatcttct
    31321 tcgccttcaa aagggaatgg ccccatgcca agaatgccgt tttctgattg tagtgttaca
    31381 tttattccct tgggaatata attagcgact aaagtaggga tgcctatgcc aaggtttaca
    31441 tacatgccgt ctttaacttc tcgggctatt ctttttgcga ttgcgttttt atcgagcata
    31501 attaattaga taattagatg atgagataat ttgataattg aatgtgcggt gctattttac
    31561 aatttattct ttgaggttga aatgatttta ttgatgattt ttttaattac ttctaattcg
    31621 attaataaat cagtagtgtc tggataacat tcggcatgct tacacaaaag aagccaatag
    31681 taagtttctt cagtttcttt agcagctatt ttaaatttgt gaataaagtc tggttcactt
    31741 tccgcatttt gagcttcata agcattggct cctattgaag ttgctgattt taagatttga
    31801 tttgcaatga tatattggtg atttgaattt aatatttctg aaagatgaat gatttttaac
    31861 gaaaactcaa aagtgagttt caggatttta ttatcttcaa aaggagaatc aaattttctt
    31921 gacatttcaa gtaattatct aattatctaa tgttcaaatt atctaatcac ttcttcctca
    31981 ccgttctctg ttctattctt ttctcatatt tctcgccttg gaaaatacgg tgaacgtaaa
    32041 ttccgggtgt gtggatttga tcggggtcaa gtgttccggc agggactaat tcttcaactt
    32101 ctgcaatggt gatttttcct gccatagcca tcattggatt aaaatttcgc gctgttgaac
    32161 ggtaaacaag atttccttct gtgtcgcctc tccaagcctt taccaatgca gcatcagaat
    32221 caaatgcata ttccatcaga taatcttttt caacgccatt gtaactgaaa cgtcttactt
    32281 cttttccttg tgccacttct gtgcctacac cggcaggtgt aaagattgca ggcatgccat
    32341 agccggcagc catacatctt gtagccaaag ttccctgtgg aattaattca acctccattt
    32401 ctcccgtcag catcagtctt tcaaattctg cattctcgcc aacatagctt gcaatcattt
    32461 tcttgatctg cttttgctgc aagagtaatc ccaagccaaa atcatcaaca cctgcattgt
    32521 ttgatataca ggttaatcct ttaatgcctt ttcgaactaa tgctgcaata caattttcgg
    32581 gaataccaca aagcccaaag ccaccaacca taagtgtcat gccatcacga aaatctttta
    32641 aagcctcgtc aacatttttt acaactttat tcatattatt tagagaatta attggatgaa
    32701 ttattgtgtt attgaattat tgatttaatt taaaatgtgt ttagattgat tcttacaaag
    32761 taaaaagtaa tattacaatg tttcaaattt aaatttatcc aataacattc taatataaca
    32821 tcaaatcatt gatttactta gatattcatt tcagtcttat ataactttca aacctttcaa
    32881 ctttaaaaca ttcaaactta ttactttctt acaccacgct cttttgccat tttttctaat
    32941 cgttcctgaa acttcgattt tttaacaggc ttctttttgt tttcctgaat ttttgcatga
    33001 atagcttttt catcaacaaa agatttaaag agatattgct gtccgaaggt aatcatattt
    33061 gcaagaaaat aataatagct caatccggaa gagtaattgt tgaaaattcc aagaaacatt
    33121 atcggcatca ggtacatcac atatttcata gaagggtttg tggcagcagt catttgcata
    33181 ttcatgcgtg tatagatgag tgttgacact gtcatgagaa ttgtaaataa actcacatgg
    33241 tctccataaa acggaatgtt gaaaggtaaa tcgagaatag aatcataggt gcttaaatct
    33301 ttcgcccata aaaaaccttc ctgtcgtaac tcaatagatg caggaaagaa gcggaacatg
    33361 gcaatgagaa taggcatttg caacaccata ggtaagcaac cccctaacgg acttacaccg
    33421 gctttgcggt aaagtgccat tgtttcttgt tgcaacttca ttgggtctgc tttaaacttt
    33481 gcctgcaact ccgacatttc aggttgcaac actttcattt ttgcagtact gatgtatgac
    33541 ttgtaagtta acggcaacaa cacaactttg atgagtatgg tcagaagtaa aatgataata
    33601 ccataattga tattaaaact attcaggaag ttgaatactg gaataacagc aaagcgattt
    33661 acccaaccaa aaattcccca tcctaatgga acaagttttt ccattccgat gtgatattta
    33721 tttagcgttt gatagtggtt aggaccataa taaaaactca ttcctgtgct ttcattattc
    33781 tgatgactga atgggattgt aaaattggcg tgcatcattc gcacagcacc ttcatcatta
    33841 attactgtac tattaattct tgttgggctt tcaaaagcct ggtcagcaat taatacactt
    33901 gaaaaatatt gttgcttaaa tcctacccat tgaaccttgg tttttaattc tgttccatca
    33961 tccttagttt cgctcaaatg gtcaacttct tcatcagtat agcgatagta aattgttgaa
    34021 acagcacttt catttttatg gcttttttcc tgttgcagta atttctgatt ccaatttaat
    34081 tccatataac ctgcattgga agctaccaat gaccccatat tcacgaagtt aattttaaag
    34141 cctacacgat aatcatcacc tttcaatgaa taaacatatt caatgtaacg gccttcaccg
    34201 gcatttaact ttatagaaac agactgcgag tcttttcctg tcacggtgat tccttttgca
    34261 ggtaaatcaa aatacaaatc tccggtagaa atcattttgt tttctgaaaa gaagctcagg
    34321 ctgaattgag agctatctcc attgttcaac accaaaggtt ttccctcacc ggttttatat
    34381 ttttttaatt caacataatc aatctttccg cccttgctgt taagctttaa tttcagcaaa
    34441 tcactttcca aagtaatact tttgctttca ccttgcgcag ctaatgcaaa tgctccaaaa
    34501 ttatctgtta gttttgtttg tgatagtgag tcgttgttta gcgtgtcggc aactgctacg
    34561 gtgtcagctt tcagtgcagc tacgggtttg gtgataagtt tcaaactgtc ctgatgcttc
    34621 ttttcaacca atgcaataga gtcttgcttt cgttttgctg cttcctgctc ttcttttgaa
    34681 ggctgattga atattgaaaa gccaatcaac acagccataa ttaaaactac tcctaaaatc
    34741 gaatttctgt ccatattttt aaaaaacacg gcaaagataa taaatgaagc ataggaggag
    34801 gctctaagaa attcagaata tgactgtaag tcagagtgat ataaaactgt gcagtttcat
    34861 gtatttttac aaccacaacc tgttatatca tattcttgtc gttgccaaaa gaatttctgg
    34921 agtcgctgcc tctttctgaa tcagataaag agttatttgt gcagtcattg caaaaacctg
    34981 tgccggtgtc agtgaggata aatccccaaa agcatactac acatttttca ggaagttcgg
    35041 ttccttggtg cagacaagga tattttttgg agcagcgacc tgtttttact ctcgacccat
    35101 tgtttcatgc aggggcatac tatgttcaag aggcttcaag catgtttttg gagcaggtaa
    35161 tttttcaact tagcctcaat gaaaaaccaa ttaatgttct tgatgcctgt gctgcaccgg
    35221 gaggaaagac gacccattta ttatcattac ttcatcagga gtcgcttgtt gtagcaaatg
    35281 aagttattgc ttcacgtaca cctgttcttg tagaaaatgc tattcggtgg ggctattcta
    35341 atttgattat cacacgtggt gatgcggtac tttttaaacg tttggaaaat atttttgatg
    35401 ttattgtttg cgatgctcct tgctcgggag aagggctgtt caggaaagat gaagccgcta
    35461 taagtgaatg gagtctgcaa aatgttgaac attgtgcttt acgccaacaa cgtattgtaa
    35521 atgatttatg ggatgcatta aagcccggag gttattttat atattcaacc tgcacatcaa
    35581 acttgcagga gaatgaaacc aatgttcagc aatttataaa taagcataat gctgaatgtg
    35641 taaaactcaa aattaaagat tttccgggag ttgaagaatt tcaaagacaa gatgctgtta
    35701 tgtatcgttt ctttccacat aaaacaggaa cagaaatatt ttcgttagca gtgttaagaa
    35761 aacctgattt gaatgatcta aattctgaaa gagttgttgc taagaatctg aagttggccg
    35821 atatagtaca ggcagacttt gcgaaaaaac atattgacaa gggagaggag atgttctatt
    35881 ttatacatga taacaatata agatttatgc ctatatgttt aaaaaatgca ttaggtcagc
    35941 tgttaaatat gccggtaata catgcaggta cagcagcatt tgaagtaaaa ggtaaaaatt
    36001 tgttgccgtc atttgaactt gccttgtcaa cagcgttaaa tcaggaaaat ttcaataaag
    36061 cagaagttga taaatctacc atgttaaaac tactcaaagg tgaaacacgt ttgatggatt
    36121 cgttgccaat aggccagcat ttaataacat atcagaacat accattgatg tttttaaaga
    36181 aaatagcagg gcgagttaat gttaactacc cacgttcatg gtttatcaga atgaacattg
    36241 aataggttga aaaatgcttt gtaagtagtg ctgtattttt tgtaatttca ctttactttg
    36301 tttcaaacaa cataggatga aaaaaaaggc aacggttaca aaacaggaaa gtcaggcaaa
    36361 agtacagtca ggaattaatt taacacagtt aaagaaatat cttggattgt tgcttgctgc
    36421 aatagcattt ttactttatg ccaatacact caaccatgat tatactgttg atgatggcac
    36481 agtgatgcaa aacaataaaa ttgtaaagaa gggagtaagt gctattcccg aaatttttac
    36541 aactgcatat cgcaaaggct tttgggacag gaatgaaagt ttgtatcgcc cgttatctgt
    36601 agcaatgttt gctgttgaat ggcagatagc tccagaaaat ccttctattg gtcattgggt
    36661 aaatgtattg ctctatgtgc tcacagcatg ggtattgttt cgtttcttgg ttaatctttt
    36721 taaagatgac tggctgtttg ctttcatcgc aacactcatt tatgtcattc atcccattca
    36781 cacagaggtt gtagctaata ttaaaagccg agacgaaatt ctatgcttct tatttctgat
    36841 tctttcattc aacagttttt tgaagttttt ggatagggca aaaccgtttt atgctttagg
    36901 agttttgttt tattttattt tatcactcct gtcgaaagaa agttcagtca ctgcaatagc
    36961 gatatttcca ttgattgcat attttttcag acagaagaca atcggtaaca gtattaaatt
    37021 gtcatggtca ccggcaattg ctattgtagc ctatctgtca ttgcgctttg ttgttcttaa
    37081 aggaatctct aactttaatg aaatattacc catcaacaat tcaattgtca atgcgcaaaa
    37141 ctatatacag caatttgcaa ctgctgttat gattgccggt aagtatttgt atttactcat
    37201 cattccttat ccactgtcat ttgattattc ttacaacact tttcctgttg taaatttttc
    37261 tgaaataaag gcattgttgt cattgacagt ttatgtcttt ttgctgatta tggctattaa
    37321 aggatttaag tcgcgcagca gattttcttt tggtattttt tattttctgc ttaccatgag
    37381 tattgtaagc aatgtatttt tcttgataga atctgtaatg gcagagcgtt ttacctacct
    37441 gccttcatta ggcatttgca ttgcattgac ctctgttctt tttactttat ttaaagtcag
    37501 taatgttgtc agagagaaaa ttgatatcaa taagattata gcaacaaata gaatattttt
    37561 cggacttata ttaattgttt ttgttgttta tagcggaatg acagttgcaa gaaatgcaaa
    37621 atggaaaaac aatctgacct tgctgtcaac tgatgtaaag acgtgccccg aaagtgctcg
    37681 gataagatat gcctacggca gtgccattct tattgaacag gctttgaaag aaaaggatta
    37741 ttcaaaaaag atgaatcttc tggataaaag tattgagcaa ttggaaaaag gtgtagctat
    37801 tttgccaact tattcagagg cttattatca tcttggtttg gcatataagg agcgagaaaa
    37861 ttatgctaag gccattgaga gctttgagta tgcagctaag agtaaaacat ttaccgaccc
    37921 gcagtttttt atttcatggg gtgttgcatg tggaaaagca aaacgttatc aggagagtat
    37981 taaagctttg aaccatgcca ttgatcttga ccctgcagca aaagaagcat atcttaattt
    38041 gggtgtgttt tatgatgaga tgcaagagtc tgataaagcc gaaacctctt taatgaaggc
    38101 aattgaatta gattctcttt ctgacggagc ctattataat ttgggaaatg tttttgctca
    38161 cagaaataat ttcgttaaag caattcagaa ttataaaaag gctttggaga ttaaccccac
    38221 aaatgaggat gcagtaaata atactggaaa cagttatgct gccatgcagg actatgagaa
    38281 tgcaataatt tatttcagag aggcaattga actgaatcct tcgaatgcaa gagcattaaa
    38341 taatttgggt gtaaccttag tgctgacagg aaaaaaagat gagggtgaac agtatattgc
    38401 gaaggctaag gcgatagggg gataatagat gatttgatga tttgatgatt tgatcatttg
    38461 aaaatttgaa gttagagatt tagaggataa ataaattaac gaattttaaa ggtgcgaatg
    38521 aggtgaaata ctcatgaaga ggaatttaca ttagatactt catcataagt cgtatttgca
    38581 tcgtttctgt ttaattaaaa atgctgtaaa atctccaata tgtaaataat ccaatgagaa
    38641 caaagtaaat gatattgtta aatgtaaaaa ggcctttggc cacaaaactc caactattat
    38701 cctttaaaat tcttaaatta acaacagaaa acggtaatat aagaagtaat attagaaatg
    38761 gtatgtagga agtcatgcta acaaaaatat tagccgggtc tttatatggt gcaggaaagt
    38821 aaaatacatt aacactgcca cataatacgc acatataata aaacatgatt aagccaattg
    38881 gaaacattaa gagctgaatg taagtgtact tattactttc ttttcttctg aaaaattgag
    38941 taagaaaaaa gatagaagtc agtaaaaaaa atccggcaga agcaacaata aataatccca
    39001 taaaattact gtctttgggg gttttgaaat tacgtaattt ttcaaattca gcactatgcg
    39061 ggaatagtgt ttctattgca ataatgtggt cgccttttct taattcatca cttaacttga
    39121 attgaacagt agcattgtag ttgtgataaa aatacaagtt cacagtccca ctgtttaaat
    39181 cccaaatgga agaaagtaaa gttccatcac caattttttt tctacataca tgcattgtat
    39241 ctgacaaggc cgtgcagaat gctattgtag tgtcaaaacc gttttttaag aatgctactc
    39301 cgtttctgta ccttgtaagt ttatttgcag tttgttcaga cgttattgaa ggacaaaaat
    39361 ttgagataac aaaagttggt tcgtttccta ttgtcaaagt ataaggttca actataagat
    39421 attttccgga cttgtcaaca taaaaaaata catcttcaat aaaataactg tagtcatatt
    39481 tattgataaa ctcttttact tcttctactg atttacaagt atgcagaatg tcctttaaat
    39541 actttgtagg gttggttata gcctttctgt ttgtaaactt ttcttgaatc gggtggtaag
    39601 atgcaagcct ttcaaaggat aaacccattt cattcattcc ggattgtggt gcataaccat
    39661 tttctccgtc atatcttgaa cctgtaaatg ctgcaccata ttgactgttg cctttacctt
    39721 tttcaaacca aatttgtggt gtaagtctcc acgcatcttc attactgcct aatatagttt
    39781 tattcccaag cgtaatctta tagccactgc aaccaaaact atttgatgtt aatgcaaaat
    39841 atataaatgt cagcagagta tgtttaacaa actttgtttt ccttgttcct ttcggcatat
    39901 attttttaac gggtcttttg ttttattatt gttagttgtg gttgacattt tgacctaaaa
    39961 tggcgtataa caatttttgg atttgacttt catacagatt aaagattact ttaccgattg
    40021 ttatcaggaa cattttaata ttagtgatca ttcggaacac aatgctccaa gtttgcacgt
    40081 cagactgtaa tttgcaaaac ttgttttaga agtagctttt tttagttttt attttagcaa
    40141 tagataatag actaaaaccc atattcctgt aaaagttaat ccgagataaa atatatttat
    40201 attttgattt aagtcttttt taataaaaag tcggtataga atccagccta gaaaaaatat
    40261 tataaagaat gcagagagat atattcgtag catagttgtt tgtttttaag tgtgttaatt
    40321 attttcattg tatttaatca tttcagtttt aattgttggt tttgaaaaga tcacgaaacg
    40381 ggttgtatgt aacttaaaat aaattcctgt catatagcca atgttctgat tgttattgtt
    40441 ccgaaaacca tcaaaattaa aactaatcat tagctcaaaa gtaaaaagaa aatgatttaa
    40501 taagacaatt gcagcaaact atgctgtatt gaaatttagc gtttgtgtta aattacgctg
    40561 tcagaggccg tgcgacatga ttggtgaaat tagatgtgtt ctattattat taaaaacgta
    40621 tttacccttt aatgttttat tattatttca aagtcctctg aaatgcgaga gactttgaaa
    40681 gtacgaaaga gcacaaagat tcaatttatt actaccgttt attcaaagca taaagctaca
    40741 agtttgaagg tcagcccgcc tgacgcaaag catctatgta aaatcctaac ttcgaagggg
    40801 caaaacataa attaaaattt taaatagaaa ttaaaatgca aacacaacaa acatcatcca
    40861 caccaaaagt aatcattggc atggacattc acaagaaaag ttggtcagta agtatccgca
    40921 ccgatatatg cgaccaccgc acattaaata tgcctgctga tgcagatggt ttgtatgact
    40981 acataagttc taattttccg ggtcatgagg tgtccatagc ctacgaagcc ggatgctgtg
    41041 gctttagtgc agcccgtcat ttcataggca tgggctggaa agtactggta gtaaacccag
    41101 ccgatgtgcc acgcatggat aaacaaaatt atcagaagac agataaaata gacagccgca
    41161 atttgtgcaa gcaactttat aacaacaatc ttcgcggcat ctatatcccc acatcagagg
    41221 aagaggaact acgcagtttg gtgcgtcatc gtaatcagat agtaaaacaa cttcgcaaag
    41281 taaagacaca tataaaaagt atgttgttgt ttcatggcat aaccataccc atagactttg
    41341 acaataacaa ctggagcaaa gcatttatta aatggcttaa aaacctaaac tggtaaaacg
    41401 catgcgcaca gatgagtctt gacagcaaac tacggagttt agcctttcat catcaggagt
    41461 atcttaacat aggtaccact cttagagctt actgcaagaa acattacaca agggattata
    41521 atcttttaag aagtattccg ggaattggag gatttttagc ttcagcagta atagccgagt
    41581 gtggcgactt gcgcagattt gccaacgaaa aacaattcag ttcatatata ggtgtggttc
    41641 ccggtattta cagtagcggt ggcaacgaaa ccatgcttgg agtgacacca cgatcaaagt
    41701 cgctattgcg gacttacata atagagagtg catgggtagc attacgttac gatcaggaaa
    41761 tccaggcata ttaccgcaaa catgcatcaa agaatccgaa gagtgtaatt gtaaaaattg
    41821 cacataaaat ggtaaagcgt atgctgtctg taataaagaa cgggcaacca tacagagtaa
    41881 ataacacaat aaataaattg aaagaagaaa aagaaaaaca gtaaaaaaca tctttaaaac
    41941 tcgcaacaac aaaagagatt tacagactac aaaacatggg tggtagttcc aaaaggaatc
    42001 tacatcgcca agcaggtagc tgtatttata gttctctaca accacacagg tgctggtagt
    42061 tgtgccatat aggacacaag ctacaaatag gagactgagt ttgaagaaca aaaaagagag
    42121 ggaaaaaaac aatggaatag gaaaatagat tacatttgaa aacggataaa aaactcttgc
    42181 gaagcaggaa ggcaaaattc aaaattgctt tcctcttcaa aagaaaattt atctccgtac
    42241 ctgagtaggg ggatttacat aggaaccctt gttagtggca gtgcttttta tccacagcaa
    42301 cttgaatttc cgtttgaagt tgtcccacaa caagcagttt tttctgttgt tttttctgtt
    42361 tttgtttcac ttccacagca gccattctct gtcgtgtcgc aacaagttcc ttcttctaat
    42421 ttgtctgcat aaactgttat gctgtaaatg cctgaacccg attttttgta atcgtcaagt
    42481 tcttgttggt ttagatactt taataaaagt tcgtcaggca attcaagaag tcgttctttt
    42541 tttatagaaa tatttttaaa tcctgctttt ttaattattc ctaaatattg tcctttttca
    42601 cttgcacctg ctacacaacc tgcatacatt tcagatgctt ctaaaacacc ttgtggaatt
    42661 gcaccttgaa aaacaatgtc ggaaatgcta aaatgtccac caacttttaa aactctgttt
    42721 acttcattaa atgctttctc tttgttaggc acaagattca taacacagtt gctaattact
    42781 acgtctgcaa cattgtttga aatttctttc atattttcaa tttcacccaa tacgaactca
    42841 acatttttat aacctaactt ttgggtattt tgtaaggcac gaattaccat ttcaggtgtc
    42901 atatcaatac caatcacttt tcctgtttcg ccaacaattc tacgagcaat aaaagcatcg
    42961 tttcctgcac ctgaaccaag gtcaataact gtgtcgcctt cgtgaatgtt tgcaatttct
    43021 gttggaagcc cacaacctaa agcaaggtca gcacttttta cgtatccttc tacattgctg
    43081 taatcttcac tcattgagtt actaaattcg cttgtggtgc aacaacctgt ttctgttgag
    43141 caacaaccta cttgaatatt gccttttgct acatcgccat aggatttttt aatttcttct
    43201 ttgattactt ctgtattcat ttttttactt ttaatttatg gcaacatcgc catttgatag
    43261 taacacttga atacaagaaa atagacgcaa aaattatgag caaccacagc ttttatcttc
    43321 ggtgtcaatc aaatttccgt atttgtcgtt ttgatacgca caacaattta aaataagttc
    43381 tttaagttta tcctttccac gttgcactct tgattttgca cctgaatatg aaatattaag
    43441 ttttttggct aattcttttt gagaaatgtc ttgaaactct gtaattaaaa gtgcctgttt
    43501 gtagttgtca ggcagtttgt tgataaaggg ttttatgcaa cattctgcaa tggtttcgtt
    43561 taatgaatta gtttctgttt ctgaaaattg ttcttgaatt tcggtgtcgc tggaaaatgt
    43621 cttttgattc ttgaaatagt catttaatgt attacgaaca attccataaa gatattgtcg
    43681 caagttttcg gattgattta ctttttcaat attgcgaatg atttttacaa aaacttcctg
    43741 caaaatgtcg tctgtgtcag ccgaatttct tgttttgttg tagataaagc ctttgagctc
    43801 tttattaaaa tttgtccaaa tattagtaac gtctgttgtc atattctgtc tgtaaaatta
    43861 attgtccaaa cgtaagacgt tcaaagtttg aaaaagacgc aggttgtttc ggggtctcac
    43921 agcattctga ctaacgtcaa atttccacac tacaaatcaa ttagatacaa atcct
//
LOCUS       DAOOOL010000005        42463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_59_length_42463_cov_127.150868, whole genome shotgun sequence.
ACCESSION   DAOOOL010000005 DAOOOL010000000
VERSION     DAOOOL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 42463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42463
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_42463_cov_127.150868"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="PKU82_00860"
     CDS             <1..2020
                     /locus_tag="PKU82_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ81452.1"
                     /translation="NIITIKIIMKKLSLLLLSIACIFSVTTMDAAKAKKSKTAKADKA
                     FNAGKYAPAADLYKKAYGKQKSKLVKAEIAYKAAECYSKMSNSKEALNWYEKSAKDNG
                     KNPDAVLKYAQALKTNGRYDEAVAQFNAFKALEPGDNRGQDGATSSETSQQWKDKPTK
                     YKVENVAALNTKYFDFAVAVSNLEKNTLYFTSSRAEASGNKNDAWYGEKFYDIFKSAQ
                     DNNGKWSIPTPISEAINTAASEGAMTFDAKGNVMYFTRCQKSDEKGKEGQCKIYKSVY
                     DGAKWGTPELLPFNSDNYTCGQPSITEDGTTLYFASDMPGGAGGKDIWMTKNENGAWG
                     TPVNISSINTVGDEMFPYIAPNGKLYFSSNGHMGMGGLDIFSATSENGAWANVTNLKY
                     PINSSWDDFGFMFNSATTGYLTSNRDGGQGQDDIYSFVVPPANFAVYGRVYDTDTKEA
                     IAGATVELFGSDGTQLSVKTQDAGTYRYELKPDVDYKVSASFTGYLTQFKELTTKGLD
                     ESKDFEQNFDFPLKSTSKPIALPEIFYDLDKYSLRPESKAALDGLIKVLDDNPTITIK
                     LTAHTDFRADDNYNLALSKKRALAAYNYLVSKKVDKDRLSWEGRGEKDPKEVENDEQY
                     LPFKRGDKLTEEFIKGLTSEELKEKAHQYNRRTEFRVLSTDYTPKKAK"
     gene            2185..3372
                     /locus_tag="PKU82_00865"
     CDS             2185..3372
                     /locus_tag="PKU82_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOZ81453.1"
                     /translation="MKDQNKYSQRGVSADKEDVHAAIKNVDKGLFPKAFCKIIPDYIS
                     NDKDYCLLMHADGAGTKSSLAYAYWKETGDISVWKGIAQDALIMNIDDLLCAGATDNI
                     LLSSTIGRNKNLISGDVIAAIINGTEHLVAELKNYGVNITTTGGETADVGDLVRTIIV
                     DSTVMCRMKRSEVVDNANIVAGDVIVGFASFGKATYETEYNSGMGSNGLTSARHDMFN
                     KSVGIKYPETFDNSLPEDVVYNGKYALTDNIILDDGYGKIEMHAAKAVLSPTRTYAPL
                     VKKIFESYRKNIHGMIHCSGGGQTKVLHFVDNLHIIKDNLFPTPALFRHIQQAAGTNW
                     IEMYKVFNMGHRLEVYCPVAIADELISIAAEFKIGAKVVGRVENCAGKKLTINSPYGK
                     FEY"
     gene            complement(3369..3728)
                     /locus_tag="PKU82_00870"
     CDS             complement(3369..3728)
                     /locus_tag="PKU82_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOZ81454.1"
                     /translation="MKKYFFLISILTLSIVSCTHKNKEVIIPEGTLSKDSMIQILADI
                     HLAESEATLHPYNDSLGIINLPAYYKYIFEHHRIDTAVFSHSMNFYLANPELMNTVYS
                     GVIDELSKRHARYQKNN"
     gene            complement(3738..5075)
                     /locus_tag="PKU82_00875"
     CDS             complement(3738..5075)
                     /locus_tag="PKU82_00875"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOZ81455.1"
                     /translation="MQTIIHNVRIVNDNKIFDSDIYFKNGIIERIDPSINVKHKVDEI
                     KGDGLILLPGIIDDQVHFREPGLTHKGDIASESAAAVAGGITSFMDMPNTVPNTLTQE
                     LLEEKYRLAQKKSWANYSFYMGAGNNNLDEVLKTNRENVCGVKIFMGSSTGDMLVDNP
                     TTLDKIFSQSPMLIATHCEDEQTIRQNTEKYKSLYGDKLNASHHPLIRSEEACYLSSS
                     FAVNLAKKYNTRLHILHISTARETELFSNNVPLKDKRITSEACIHHLWFDDNDYASKG
                     NFIKWNPAIKTQVDKEAILNALLSDKIDVIATDHAPHTLDEKQMPYLKAPSGGPLVQH
                     ALVAMLELFHQGKISLEQIANKMCHSPAICFNIKNRGFIQEGFAADVSLVNLNEPWTV
                     AKSNILYKCGWSPFEGTTFQSKVKHTFVNGVHIYNDGHLQLSTNGQRLLFSSH"
     gene            5229..5302
                     /locus_tag="PKU82_00880"
     tRNA            5229..5302
                     /locus_tag="PKU82_00880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5263..5265,aa:Pro,seq:ggg)
     gene            5433..7109
                     /locus_tag="PKU82_00885"
     CDS             5433..7109
                     /locus_tag="PKU82_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOZ81456.1"
                     /translation="MALSRIWSYMILFAVAVAGYRYMQGDKNIFNDMVVEKKLAAGES
                     FSKVSYYVIGQGIDSVLKEPKLKNANWVKTDKFSDAQVIITTADLKNNLREQLNNQLA
                     KISFANINTQNKFTADKSKLIYSEELITDSTLNITNNLLQANVVLTSEVNTDSLRNAW
                     TAISNRQKIVGSNEVFLWLPKNIDGIFETGKFAVTIAIGLIGTLALFMGLLNIAERAG
                     GINIISRLISPFLSKLFPSLPKNHPSFGHMVMNFSANMLGLDNAATPFGLKAMQSLQE
                     INTNKDRASDAQIMFLALHASGLTLIPVSIIAVRSANGSHNPNEIFIPLMLTTFVCTL
                     TAMIVTSLKQKINLFQRGLLLPVAGLSLTLSLFVWFLSSLRPFALDQFSDAFSNGAIL
                     LVFFGIIAGGLYKKINLFDAFVDGAKGGFETAVRIIPYMVGMLVAVSMLRTSGAFDLI
                     INPVKHLIAISGMDTRWAEGLPTALLRPFSAGGARGFMIDAMRTFGPDSFIGKLVSVL
                     QGSSETTFYVVALYFGSVSVKDTRYAIPVMLLADLAGVIFSIVICYLLWA"
     gene            7249..9441
                     /locus_tag="PKU82_00890"
                     /pseudo
     CDS             7249..9441
                     /locus_tag="PKU82_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
     gene            9469..9984
                     /locus_tag="PKU82_00895"
     CDS             9469..9984
                     /locus_tag="PKU82_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ81457.1"
                     /translation="MTGTISLDVSHSWMQFGISHFLTTANGTLAVDTGYINITNDLAT
                     SSIFVQLNVKSIDTKNTKRNNHLLSSDFFDAEKFPSIVFKSNKIEKAAEGSAFRYIAK
                     GELTIKNITKPVELRFNYMGALPNEYAAKDAHTFVGEADLDRIDFGVGESGPTVGNKV
                     KATFSVEGYKI"
     gene            complement(10064..10777)
                     /locus_tag="PKU82_00900"
     CDS             complement(10064..10777)
                     /locus_tag="PKU82_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOZ81458.1"
                     /translation="MIVDLLIPCYMDQVFPETANNVVKIIEKLGCGVNYNVEQTCCGR
                     TAFEEGYQKECKAVGEKLIKEFQNERYIVCPGAYCSGMIKNIYPQMFHNSALHNEYKS
                     VQKNIYEFTDFLVNILNVTDVGARFNGKVVILDSCKAINELTVKDSVRTLLSKVKGLE
                     WIESTDSCCGYSPSFTEKYEAISVDIGKRKILELTQTGANTVISTDYTCLMHLKSIAD
                     NEGSSLQFMHIADMLASGW"
     gene            complement(10807..12096)
                     /locus_tag="PKU82_00905"
     CDS             complement(10807..12096)
                     /locus_tag="PKU82_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ81459.1"
                     /translation="MEKNNPKVLRAWTFYDWANSVYSLSITTAIFPIYYNSITSANGS
                     DQVTFLGRTFTNTALYSYALSFSFLVIAFLSPLLSGIADYKGNKKFFMRLFCFLGSTS
                     CASLYFFKDLANLDIGIISFILASIGWAGSIVFYNAFLPEIATADMQDKVSAQGFALG
                     YIGSSLLLIFNLAMILNPSWFGITNSGMASRISFLMTGVWWFGFSLFTFKYLPEKTST
                     HNTNEQLLTKGFNELIKVLKQLQSLPMLKRFLISFFFYNMGVQTVMYVASLFGTKELH
                     MRDDELIITVLIIQFVAIAGAYLFAFLSSKAGNIKALLIAVTIWIGICWAAYFVATSV
                     QFFVLAFAVGMIMGGIQSLSRSTYSKMLPKTQDHASYFSFYDVCDKIGLVLGTVAYGY
                     IEELTGSMRNSILVLMTFFVVGLFSLLSIRNFRLQNT"
     gene            complement(12113..12886)
                     /locus_tag="PKU82_00910"
     CDS             complement(12113..12886)
                     /locus_tag="PKU82_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81460.1"
                     /translation="MGFRLWHCRSTGFKKLNWLLFLSQILLPIFVASFLISGCKKKSD
                     DSPSPFLGITVYNDFKTHPDTFIFKAESTIPCNYKWNFADGSTEVSGDSVMHIFDYGF
                     YNVVLKGETAEGKTATTIKGINASPYTKANIVSLSLNSWPVHKSDGTTWDSDGSAADI
                     YCQIWNGNTYVSTTVSTNAPQGTANFNFITPFAITQFDKSIIIKVFNYNFSPETDDLM
                     ETVVIEKNLTPLMTNQLPYAGQQLFNGGQTNGALNFIWQ"
     gene            complement(12886..13287)
                     /locus_tag="PKU82_00915"
     CDS             complement(12886..13287)
                     /locus_tag="PKU82_00915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOZ81461.1"
                     /translation="MSETITPIQIRFSDIDWMGHVNNAVYLSYIEKARIDFSERLPLN
                     INWRETGFILAQTQINYRHPALLNDNLSVRTWCTRVGTKSFDLGFEVFKTTDNNKIIL
                     ADGLTVLVCYNYRLSVSENIPEEWKKLLLKK"
     gene            complement(13290..14543)
                     /locus_tag="PKU82_00920"
     CDS             complement(13290..14543)
                     /locus_tag="PKU82_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81462.1"
                     /translation="MMNRILLLMLAVISYGQSFTQCLDPYYPRIYQTYVERDISYGSD
                     TAYNNTVANLQMNIWKPIGDNNTARPIILWIHGGGFFTGNKNDMDVIAQAYAERGYVS
                     ATMSYRLGFYGNLFFGAPFTFDSSEVIRAAYRAVQDAHGAMRFLKARAAIDSSNINLA
                     FVGGASAGSITAMHFTYVDKQSEAPINGGAIGDVTTLFGNVQRNDLGSIFGRLNLNGQ
                     SSNVLAAVNLFGALLDTSFIESNTDPALFSYHQTGDGTVGCGYMPGLWNQPMNVSQNY
                     PHLFGSCVINDRMNNLGFSSQHYQALLYNGNVHGIHDDALIDTLVARFLSDILCENIL
                     STNTVDMPDIDVYPNPAKGQINISIPGKTFTCSLFDVQGRNIIPSNSLLHDYITLNTE
                     GISKGMYLLNIITDKQLISKRIIIE"
     gene            14950..15099
                     /locus_tag="PKU82_00925"
     CDS             14950..15099
                     /locus_tag="PKU82_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ81463.1"
                     /translation="MNLNYCIDKKKLKVFAYCIMPSHIHLIAKTEDNVNVSDVLRDFK
                     SYTSK"
     gene            complement(15623..16558)
                     /locus_tag="PKU82_00930"
     CDS             complement(15623..16558)
                     /locus_tag="PKU82_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HOZ81464.1"
                     /translation="MSKLDEAKKILKELGLPTAQQNEISAYTLLALCGIKPRNSWSKA
                     TRKSLKVTKGIMAFVLDVHKKEYAPNTRETFRRQVLHQFVQARIADYNPDNPKLPVNS
                     PNAHYAITNEALEAIQNFGTKDWKKAVEKFKSEAGELAKRYKKERKQNLIPVKLSNGK
                     TIKLSSGKHNEVQAAIVHNFAARFANGGSILYLGDTAKKDLFVDEKKLKELGIPIDQH
                     SKLPDVVIYDEKKNWLFLIEAVTSHGPVSPKRIVELEEFLKGCKAGKIYVTAFPDFTE
                     FKKHSNNIAWETEVWLADTPDHMIHFNGDRFMGPR"
     gene            complement(16555..18141)
                     /locus_tag="PKU82_00935"
     CDS             complement(16555..18141)
                     /locus_tag="PKU82_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOZ81465.1"
                     /translation="MADKKLIEKIADELPSHCADRLGVNYAQSVNQEHKKTNGQFFTP
                     IEIAGLMGTFAEVSESSLRILDPGCGTAILTCALIESIVQNNSKLQEIDLIVYETDKA
                     LIPYTKESLDYLRSWLNAKNIELKFSIHTDDFILENAGCLTDNGNLFSQTINPFDIIV
                     SNPPYFKLPIDDKRAIAAKAVVNGHPNIYAIFMAISARLLKENGQLIFITPRSYASGS
                     YFKLFREYFFKIIEIDNVHLFVSRKDTFNRDKVLQETVIIKGTRKEKTNPKHKVHVYS
                     SHGLKDIDSPIIKSFAQKELIDLSSNEKILYLPTSDSDEAILNVFKNWSGNLNKYNIQ
                     ISTGPVVAFRSKDHIHDTYQNGTVLLAPLFWLHNVQQMALDWPMPKPEKGQYVRVHDE
                     SKSILIPNKNYVLLRRFSAKDDKSRLVAAPYFCNFIEAEYIGVENKVNYIYRPKGHLE
                     RNEVIGLCALLNSSLFDSYFRIFNGNVNVSATELREIPLPPLETIKEIGNSVILSNDF
                     SVDKANKIVNEQFEFTLATI"
     gene            complement(18131..18439)
                     /locus_tag="PKU82_00940"
     CDS             complement(18131..18439)
                     /locus_tag="PKU82_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ81466.1"
                     /translation="MKNIAEIIRELREQNGLLLRQVAAEIEIDQALLSKIERGERMPT
                     KDQVIRLAKFYKVDLNEFLIAFISDKLVYELQNEEVALKAMQVAEKKINYIAKKKNGR
                     "
     gene            complement(18817..19356)
                     /locus_tag="PKU82_00945"
     CDS             complement(18817..19356)
                     /locus_tag="PKU82_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ81467.1"
                     /translation="MTTLTKTTWGIDQAHSEITFKVKHLMISNVKGNFAKFEGTVQSE
                     DSNFDNANINFSMDASSITTGDTQRDTHLKSGDFFDAEKHPSISFKSENFSKTGDDTY
                     ALNGQLTMKGISKPVKLIAEFGGMMKDPWGQEKAGFTLSGKINRKDWGLNWNTALETG
                     GVLVSEEVKIAAEIQLVKK"
     gene            complement(19390..20112)
                     /locus_tag="PKU82_00950"
     CDS             complement(19390..20112)
                     /locus_tag="PKU82_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOZ81468.1"
                     /translation="MENKVIHRAENRGNADHGWLKAKHSFSFASYYNPNQMHFGVLRV
                     LNDDHVEPGMGFGTHPHDNMEIITIPLAGALEHKDNMNNHGIIKYGEVQVMSAGSGVT
                     HSEFNASKTDAVKLFQIWLYPDKENVKPRYDQLSLNVNDRKNKLQTIVSPDTEKEGLW
                     IHQQAWLSMGNFDKNKSTSYKIKKEGNGVYAMVVKGEFEINHNLLHERDAIGLWNTSE
                     INFIAKTEDAEILLMDIPMQLN"
     gene            complement(20226..20828)
                     /locus_tag="PKU82_00955"
     CDS             complement(20226..20828)
                     /locus_tag="PKU82_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ81469.1"
                     /translation="MAKTDSNKPFNLILKNISRYISLSEEEEAIFTSLLSARIVRRKQ
                     FVMQAGEVFTASCFVNSGCLRAYNIDKSGTEHILQFAPPGWWIGDFYSSITGRPGILF
                     IDAIDESEIVVLTKENRDLLFDKIPKFERFFRILIENSMASQQERILDKLSLSAEERF
                     EKFCLKYPALVSCLPQKYIASYIGVTPEFFSKMRARMTKN"
     gene            complement(20812..21390)
                     /gene="lpcA"
                     /locus_tag="PKU82_00960"
     CDS             complement(20812..21390)
                     /gene="lpcA"
                     /locus_tag="PKU82_00960"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOZ81470.1"
                     /translation="MSEIIRQHFEEAKKVLETFLSVDKNIAAVTKAGELMTASLAAGG
                     KIISCGNGGSMCDAMHFAEELAGRYRNDRKPIAAISISDPSYMSCVGNDYGFNYVFSR
                     FVEGVGNKGDVLLAISTSGNSANVLEAAKVAQAKNIIVVALTGKDGGALKNHCDVEIR
                     VPHSGFADRTQEIHIKIIHALIDYIEQHGKNR"
     gene            21524..23104
                     /gene="lnt"
                     /locus_tag="PKU82_00965"
     CDS             21524..23104
                     /gene="lnt"
                     /locus_tag="PKU82_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOZ81471.1"
                     /translation="MQVKKKLILSLLSAVMLSLGWPERGLTPLIFIAWVPLLLLEEYS
                     HQKKEQKKFGLFGWTYLTMLGWNALTTWWVCNASFGGGLFAIFCNSLFMTCVFYAFHI
                     VKKRKGHVWGYTALIIFWVAFEYLHLNWELSWPWLTLGNVFATKPQWIQWYEYTGVLG
                     GTVWVLLVNILITRIFVASADKLKRSVLYATVAIAFPGLISLLRYYTYKETENPVNVI
                     ATQPNIDPYGEKFSGMSNEEQLIPVLRLANLELNDSSDVILAPETAIANSVWEDELEL
                     YPEVKLIRKYLQVNPRATLIIGASTNRAYLNGQKPSSTARKFKDQDGYYDSYNTALLF
                     EDGKPAQIFHKSKLVPGVEKMPYPKIFGFLEKLSIDMGGTAGSLGIQDEPTVFTTKDK
                     MIIAPVICYESIYGGYLAKYVRKGAQWIAIITNDGWWGNTPGYRQHFNYARLQAVSLR
                     RSIGRSANTGISGFINQRGDAFQQTPYWHDAVVRQTINKNDKLTFFAQYGDYPGLIAS
                     ILVLPVIVITFFGKRKNQ"
     gene            23142..24545
                     /gene="lpdA"
                     /locus_tag="PKU82_00970"
     CDS             23142..24545
                     /gene="lpdA"
                     /locus_tag="PKU82_00970"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ81472.1"
                     /translation="MENFDVAIIGSGPGGYVAAIRCAQLGFKTALVEKYDTLGGTCLN
                     VGCIPSKALLDSSEHYFNAVNKFSEHGIKIKDIKADMEQMIKRKAQVVKQTCDGISFL
                     MKKNKVTVLTGHATFVDSKQLDVVDAGGKSNRVGATNFIIATGSKPSSLPGIEIDKKR
                     IITSTEALELKTIPGHLVVIGGGVIGLELGSVYARLGAKVTVIEYMNAIIPTMDRALG
                     KELQKVLSKEGFTFLLGHKVQSVKSSAKQVEVVGESSSGEKVNIIGDYCLVSVGRKPY
                     TEKLGLDKAGVALDERGRIITDEKLQTNVPGIYAIGDVIKGAMLAHKAEEEGVFVAEQ
                     LAGQQPHINYRLIPGVVYTWPEVASVGYTEEELKESGVKYKTGNFPFKASGRARASMD
                     TDGFVKVLADSTTDEILGVHIIGPRAADMIAEAVVAMEYRASAEDIARICHAHPTFTE
                     SFKEACLAATANRAIHI"
     gene            24575..25141
                     /locus_tag="PKU82_00975"
     CDS             24575..25141
                     /locus_tag="PKU82_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOZ81473.1"
                     /translation="MNIQIKEIITVSMILFSVIDIIGSVPVIIGIRKSVGTIHPEKTS
                     LVALMIMIVFLFVGENILSLIGLDVSSFAIAGSFILFFLALEMILGVKLYKDEMPASA
                     SIVPIAFPLIAGSGTMTTLLSLRSQYDMLTIIIGVLLNMIWVYLCLKNLGFIERLLGP
                     TGIAIVRKVFGFILLAIAVKLFRSNLGI"
     gene            25146..25772
                     /locus_tag="PKU82_00980"
     CDS             25146..25772
                     /locus_tag="PKU82_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOZ81474.1"
                     /translation="MAKKKFYVVWKGVMPGVYHSWDDCKRQIDGFAGALYKSFETENA
                     ALTAFQKGLSGFQKKSESQHLFQVEKSSSKPIKESIAVDGAWNTKTLESEYRGVDVKT
                     GKQLFLKGPYADGTNNIMEFLAIVHALAYCKRHHINLPIYSDSMTALKWVKTKKANTK
                     LASTGRNEELFELIDRAEEWLKNNIYANKLLKWETQIWGENPADFGRK"
     gene            25975..26160
                     /gene="ccoS"
                     /locus_tag="PKU82_00985"
     CDS             25975..26160
                     /gene="ccoS"
                     /locus_tag="PKU82_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HOZ81475.1"
                     /translation="MSVLIILIGASLVVAAGFLVSFFWAVRSGQYDDDYSPSVRILFD
                     NDKTLTSDKSNSNITDN"
     gene            26165..28291
                     /gene="ccoN"
                     /locus_tag="PKU82_00990"
     CDS             26165..28291
                     /gene="ccoN"
                     /locus_tag="PKU82_00990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HOZ81476.1"
                     /translation="MTIEKFRYDNKIVAMFAWATVIWAIVGMLVGLLAAFQLPFPALN
                     FDAHTTFGRIRPLHTNAIIFAFVGNGMFMGIYYSLQRLLKARMYSDVLSYIHFWGWQL
                     IIVGAAITLPLGITASKEYAELEWPLDIAIAIVWVIFGINMFGTLVRRRERHLYVAIW
                     FYIATVVTVAVLHIVNSLAIPVTLFKSYSLYAGVQDALVQWWYGHNAVAFFLTTPYLG
                     LMYYFLPKAANRPVYSYRLSIIHFWSLIFLYIWAGPHHLLYTALPDWAQSLGVVFSIM
                     LLAPSWGGMINGLLTLRGAWDRVRDNPELKFYVVAVTAYGMATFEGPMLSLKSVNAIS
                     HFTDWTIAHVHIGALGWNGFLTFGILYWLIPKLFNTSLYSKKLAGIHFWLGTLGIVFY
                     AIPLYWAGITQHFMWKEFTEDGVLRYPNFLETVVQVVPMYKLRALGGSLYLLGSLVMV
                     YNLIKTARAGKMVANEDAEAPALEKNYVAHDDHWHRWIERRPAQMLIGSLIVILIGGM
                     VEIIPTFMVKSNVPTIASVKPYTPLELQGRDIYVREGCYTCHSQMVRPFRYETERYGE
                     YSKPGEFVYDHPFQWGSKRTGPDLHREGGKYPNSWHYNHMLDPQTMSPGSIMPSYAFM
                     IDDDLDTTTTAAKIRAMQKLGVPYPEGYDAQANKDLMKQANSIASNLEKDGIKVMGNK
                     EIVALIAYLQRLGVDIKAEKTTASVN"
     gene            28518..29663
                     /locus_tag="PKU82_00995"
     CDS             28518..29663
                     /locus_tag="PKU82_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ81477.1"
                     /translation="MFISLSAWAGDTQATETTKVAAAQPPDWLMNANTYLIAFVVIIL
                     LATILALYRSSLKLLKKITSFCFIMFVSGSAFAAEEAAPAEATKVTAIQPPDWLLHTN
                     TYLIAFIVFILVVTIVVLFKTNMKLIRMIAPHAFDDEKQTAEAGAVSGVKNRETFMRK
                     VYLRLVDSVPVAKEQDILLDHDYDGIQELDNNLPPWWKWGFYITIIAAVIYMLAYHVT
                     GTGKLQAEEYADELAVAAKQKEERMKNSAENITEENVTVLVDAAEIAKGKDIYEKNCS
                     ACHMADGGGMVGPNLTDSYWLHGGGVKNIFKTITYGVPAKGMISWQAQLSPKQIQEVT
                     SFIMTLHGTKPAAPKEPQGDEWKDEPAAGATATDSAAVATTAEAVKK"
     gene            29681..31084
                     /gene="ccoG"
                     /locus_tag="PKU82_01000"
     CDS             29681..31084
                     /gene="ccoG"
                     /locus_tag="PKU82_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOZ81478.1"
                     /translation="MVENKMEHIDESFRDSIATVTEDGKRAWIYPKKPKGRYYNARTW
                     VSIVFLIIFFALPLIKINGHPFFLLNVVELKFVLFGIAFFPQDFYLFVLAMLTFVVFI
                     ALFTALFGRLWCGWTCPQTVFMEMVFRKIEYLIEGDFMKQKALDKRAWDAEKIRIKTT
                     KHLVFFFIAFLISNTFLMYIIGFDEWKKIITDNPFDHIGGLFATVIFAGVFYGVYARF
                     REQVCIVVCPYGRLQGVMLDPNTVVIAYDYKRGEPRGKLSKDNEEHGDCIDCNQCVQV
                     CPTGIDIRNGTQLECVNCTACMDACDDIMDKIQKPRGLIRYTSENAIKNNAKFKINSR
                     IIGYCVVLLVLFSALVVLLARRSNIETTILRASGLLYQKPDESHISNLYNIALINKTF
                     GNMPIELKIVEPKGEIKWVGNGITHLKEQSVAQSTFFLILPKSEIKKTKTKVVFEIWS
                     EGEKIDRYETSFLGPNN"
     gene            31090..31527
                     /locus_tag="PKU82_01005"
     CDS             31090..31527
                     /locus_tag="PKU82_01005"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOZ81479.1"
                     /translation="MKFNWGHGIALVLIIFASSILVFVYVASKQKNELVTDKYYEKAV
                     AYQQRIDAEKNALNENADVKYNINSGQLMLTLGRNIPNANGTIQFYKPDDATKDFNTS
                     FQVNDTGFYSATIPQLTHGQWKVNVSWNVDSKEYFYEKKIFIP"
     gene            31527..32255
                     /locus_tag="PKU82_01010"
     CDS             31527..32255
                     /locus_tag="PKU82_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ81480.1"
                     /translation="MNEYTFYYAALLLGFLGSFHCVGMCGPIALMLPANKLDNRRAAF
                     FGRLFYNIGRMITYMFIGMLFGLLGAAFVLNGFQKQLSIITGIILLLVAIFTLIPSTR
                     KKVLSVSSKMNIPFRGVLRKLFSGKTALHHFGIGVINGFLPCGFVYVATAGAINSGSV
                     LNSVLYMALFGLGTFPIMLLVSYAASWCSFSFSRYVTKATPWISAALALFLIYRGAFM
                     NINADCHKHSHPPTMVECSKPETL"
     gene            32347..35370
                     /locus_tag="PKU82_01015"
     CDS             32347..35370
                     /locus_tag="PKU82_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ81481.1"
                     /translation="MSHKLLDALMHLFAILANAEKFSLEGRSIVESFLRQQISRAYVL
                     KYLGLFDEYLENIRGKATEGKLKKRLSVNSVKVLRICTDINAELDTRQKHIVLMRLIE
                     FVFTSEKIIDSQEKEFLYTVAEVFHIAPDDVQLCIELACNNAEEKYSDTPKYLVLSSQ
                     DDNSSLNTKTQIISGLNGVFRFLFLKNAGLLFVRYHGDESFTLNGLPLNDDLCYVFSA
                     GSVIRGSKVNTIYYTDIIRLFSNFKQQSLLHFDVQHVYYDFSNGKRGLNDVAFSATSG
                     NLVGIMGGSGAGKSTLLNILNGNNRPTQGKVTVNGIDLHRESHLLEGMIGYVPQDDLL
                     IEELTVYQNLFYNAKLCFGNLTDSEIDERVKALLLNVGLSEASHLRVGNALSTSISGG
                     QRKRLNIALELIRAPEVLFVDEPTSGLSSLDSENVMDLLKQLANHGKLVFVVIHQPSS
                     DIFKMFDKLFVLDIGGYPAYFGNPVDSLLYFKQRASFADADYSECGTCGNINPEQIFS
                     ILESKVMDEFGFPTQQRKLTPADWYQLFKEHNSNSSKSNQRQPVQGSANQKPGWLQQL
                     KVYMTRDMLSKFKNKQYMLITFLEAPVLAIILAWFLRYFNPDGSYVFSENKNLVAFLF
                     MAVIVAIFMGLTVSAEEIIRDRKMLKREKFLNLSRSSYLYSKMMVMFLISAIQTFSFV
                     IIGNLIFGIQSMTFDYWIMLFSVSCFANMLGLNVSSMFDSAVTIYILIPFLIIPQIIL
                     SGVMVKFEDLNPHLTSQRHVPLIGELMASRWAFEALAVNQFSNNPYQKYFFNVDRQMS
                     EATYKKDFWLQKLNDIAEGVSKNKSKNPKVDVELITNELHKEALVNKNFKTAIDFTKA
                     NASEIASQIKPLTDILRNYYIEKYNNASAAKDSFVKKELKHSSVAMLNKQKEKYTNNA
                     LNDLVLNKNDFTILVQDGNSIVQRFQPVYMQPDPTTWFRSPLYVYEKNFFGMKIHTWY
                     VNLFIIWLMSFILMTTLYFDSFRKLIHWVGHSSKR"
     gene            complement(35514..38072)
                     /locus_tag="PKU82_01020"
     CDS             complement(35514..38072)
                     /locus_tag="PKU82_01020"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOZ81482.1"
                     /translation="MLIETITTESQMTTYEYDSVLKASLEYFEGDELAATTWMNKYAM
                     KNKEGKFVERTPHDMHLRMAREFARVEAKYNLLNDGSRTSHLSEYGKKREQLTTEKIT
                     ALFDRFRYVIPQGSVMSALGNPFMIASLSNCIVLPELHDSYGGIFFADQQLAQLYKRR
                     CGAGLDLSTIRPEGMRVSNAAGTTTGAVSFMERFSNTTREVAQLGRRGALMLTLDIAH
                     PDVEKFITVKQDLSKVTGANISVKLSDEFMVAVNENTDFNLRFPVDSTDPYIQKKVNA
                     RELWKTIIACAHRTAEPGIIFWDRQHHYSTSSVYPGFKNVSTNPCSEIAMQGGDSCRL
                     IAINLLSFVDHPFTQKAKFNFTRFYEVTYESQRLMDGLVDLELEAIERILQKIESDPE
                     PDYIKEVEMRTWITLYEAGKKGRRTGLGFTALADALAAMNIAFDSDNALSIVEMMMKE
                     KCRAEFDSSIDMAIERGTFEGFDTAIEDQSEFVNMMKAELPDIYNRMMKYGRRNISIS
                     TVAPTGTLSLLSQTSSGIEPVFMLSYKRRRKVNAEDSKAKTSFVDQSGDAWQEFEVYH
                     HGLKKWMTANNSTDYSKSPYAGSTANEIDWKKRIKMQAVVQKYTTHSISSTINLPENV
                     SAETVGEIYMQAWSAGLKGITVYREGSRSGVLVKKEEKKAEPVITESSAPKRPEILAA
                     KVIRFMNADEKWIAYIGILDNKPYEIFTGKAEDAFSIPAWVSSGWIIKSKNGHGKSRY
                     DFQFNDKEGFKVTIEGLSRSFDKEFWNYAKLISGVLRHGMPIPQVVNLVENLHLYSDS
                     INTWKNGVMRALKQFIPDGTKVNDKVCAECGDKEGLYFEEGCIKCKSCGHSKCG"
     gene            38351..39727
                     /locus_tag="PKU82_01025"
     CDS             38351..39727
                     /locus_tag="PKU82_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOZ81483.1"
                     /translation="MKFKTIIEPFRIKMVEPIHLSTEEQRVGYLKQAHYNPFLLNSNQ
                     VLIDFLTDSGTSAMSSDQWSAMMNGDESYAGASSWLRFEKEVQSLTGMPFVLPTHQGR
                     AAERILYGKLGGKGKTFISNTHFDTTRANIEFSGAEAIDIPVKEGLDFASDFPFKGNM
                     DIVLLEKTIAEKGAANIGAVILTVTNNSGGGQPVSMENAKAVADICRKNKVLFVIDCC
                     RVAENSYYIHHNEAGYENKTYRQIAEEMMCLGDAAVMSAKKDALVNMGGFLAVKDADL
                     AESCKQLLIITEGFATYGGLAGRDMEAIAVGLQEVFDPGYLNYRIVSTRYLGENIKSK
                     GVNIIYPVGGHAVYVDAKSLYPHIPPHEYPGQALVCELYRIGGLRSVEIGSVMFGKYD
                     SNGKLIPAPMELVRLAVPRRVYTQSHIDYVIEIFDEIVRTKDKVKGLKIVKEPQFLRH
                     FTAHFEPL"
     gene            complement(39803..40627)
                     /locus_tag="PKU82_01030"
     CDS             complement(39803..40627)
                     /locus_tag="PKU82_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOZ81484.1"
                     /translation="MKWFLAIKKQVRPLILWMLLLLQIATIGNAQQIPPQSNPPRLVN
                     DYAGVLNDADENLLEQKLVQFNDSTSTQIAIVLIRSTGEYPVDDYAIALFRDWKIGQK
                     NKDNGVLILAAMEDRRMTIITGYGVEGALPDAICKRIIELTLKPAFKSQQYYNGLDAA
                     VTEIMQRTKGEYVSDGKNAKNNSPAWAPILFVFLIFGVVMLMKVFSVRRYAQLNNLSF
                     WTAWMLLNAASNRRNSSGGGGVWGGGGSSWGGGSSGGGFGGFGGGSTGGGGATGGW"
     gene            complement(40605..41045)
                     /locus_tag="PKU82_01035"
     CDS             complement(40605..41045)
                     /locus_tag="PKU82_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOZ81485.1"
                     /translation="MANAADYFSDEQQLLLKNAIARAEKHTSGEIRIFIDSKVKSEII
                     DRAAEVFENLKMHETKLHNGVLIYVAIENRQFAIIGDSGIHEYVSQVFWDKVKEEMIP
                     FFKRKDYTGGLLHAIEETGQKLKKYFPVAKNDSDELSNEVVFSD"
     gene            complement(41048..41638)
                     /locus_tag="PKU82_01040"
     CDS             complement(41048..41638)
                     /locus_tag="PKU82_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOZ81486.1"
                     /translation="MKRSVVIIGILLVVAFIIYRLFAGTYNSMVTADENVKGKWAQVE
                     NQYQQRMDLIPNLVNTVKGAAEFEKGTLTAVVEARASATQVKVDPDKLTPENIQKFQE
                     AQGQLSTALGRLLMVTENYPVLKANQNFLELQATLEGTERRIAVARKDFNEAAQQFNT
                     YIRKFPQTLLAGMFGFSPKGYFQSDKGANKAPEVKF"
     gene            41818..42087
                     /locus_tag="PKU82_01045"
     CDS             41818..42087
                     /locus_tag="PKU82_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOZ81487.1"
                     /translation="MIKTVAIEITGKVQGVFFRKFTQQTGRRLNLCGFVRNRSDGSVY
                     VEATGSELQLNELVAWCHKGSPMSLVRQVVVREISVIHNEPFQIL"
     gene            42129..>42463
                     /locus_tag="PKU82_01050"
     CDS             42129..>42463
                     /locus_tag="PKU82_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ81488.1"
                     /translation="MSGIKKLAGQTVLYGLSSIVGRMLNYLLVPIYTRIFSPAEYGVV
                     SELYSYSAFLLILFTYGMETAFFNFMQKEENKNKVYENCISLVSYTTTILVLLMIFFS
                     SPLAATIEYP"
BASE COUNT        12919 a         7524 c         8379 g        13641 t
ORIGIN      
        1 gaatattatc accataaaaa ttattatgaa gaagttatca ttactattat tatccattgc
       61 ctgcatcttt tcagtaacca ctatggatgc ggcaaaagcc aagaaaagca agactgctaa
      121 ggcagataag gcgtttaatg caggtaagta tgcacctgca gcagatcttt acaagaaagc
      181 ctatggcaag caaaagtcga agttggtaaa ggcggaaatc gcctacaaag ctgcagagtg
      241 ttacagtaaa atgagcaact ctaaagaagc tttaaattgg tatgagaaat cggctaaaga
      301 caatggtaaa aatcctgatg ccgtattgaa atatgcacag gcattaaaaa ccaatggccg
      361 ttatgatgaa gccgttgcac agtttaatgc atttaaagca cttgaacctg gtgataaccg
      421 tggtcaggat ggagcaacat catcagaaac ttcacagcag tggaaagata agccaactaa
      481 gtataaagtt gagaacgttg cagcattaaa cacaaagtat tttgattttg ctgttgcagt
      541 atcaaactta gagaaaaata cactttattt tacatcttca agagcagaag catcaggaaa
      601 taaaaatgat gcatggtatg gagaaaaatt ttatgacatc ttcaaatctg ctcaagataa
      661 caatggaaaa tggagcatcc caacaccaat cagtgaagca attaatactg ctgcatcaga
      721 aggtgctatg acatttgatg caaaaggtaa cgtaatgtat tttacccgtt gccagaagag
      781 cgatgaaaaa ggtaaagaag gacagtgtaa aatttacaag agcgtttatg acggtgctaa
      841 atggggtaca ccagagttgc taccatttaa tagcgataat tatacttgtg gacaaccttc
      901 aattactgaa gatggaacca cactttattt tgcatcagat atgcctggtg gtgcaggtgg
      961 aaaagacatc tggatgacta aaaatgaaaa tggagcatgg ggtacgcctg taaacatctc
     1021 ttctattaat actgtaggtg atgaaatgtt cccatatatt gcaccaaacg gaaaattgta
     1081 tttctcatca aatggtcaca tgggcatggg tggacttgat attttctctg caacttcaga
     1141 aaatggagca tgggcaaatg ttactaactt aaagtatcca attaactcat catgggatga
     1201 tttcggattt atgttcaatt ctgccactac aggttatttg acttcaaacc gcgatggcgg
     1261 acaaggacag gatgatattt attcgttcgt agtacctcct gcaaattttg cagtttatgg
     1321 tcgtgtatat gatacagaca ctaaagaagc aattgcaggt gcaacagttg aactttttgg
     1381 tagcgatgga acacagcttt ctgttaaaac acaagatgcg ggaacatatc gctatgagtt
     1441 aaaacctgat gttgactata aagtttcagc atcatttaca ggctatctga cacaatttaa
     1501 agaactgaca actaaaggtc ttgatgaatc aaaagacttc gaacagaatt ttgacttccc
     1561 attaaagtca acttcaaaac caattgcatt acctgaaatt ttctatgacc tcgacaagta
     1621 ttcattacgt cctgagtcaa aagcagcatt ggatggatta attaaagtgt tggatgataa
     1681 cccaactatt accattaaac ttactgcaca cactgacttc cgtgccgatg acaattacaa
     1741 cttggcactg tctaaaaaac gtgctttagc agcttacaat tatttggttt ctaaaaaggt
     1801 tgacaaagat cgtttgagct gggaaggaag aggagaaaaa gatcctaaag aagttgagaa
     1861 tgacgagcag tatctaccat tcaaacgtgg tgataagtta acagaagagt ttatcaaagg
     1921 attaacttct gaagaactga aggaaaaagc tcatcagtac aaccgtagaa cagaatttag
     1981 agttcttagt acagactata cacccaaaaa ggctaagtaa gcccgattga attgtaaata
     2041 aaaaagaggc tgtcttggtt aaagacagcc tcttttgtta gttaggattc agaatctcaa
     2101 aagagcttat aataactaat agaataatct ccgccatttt gaacacacac ttttttaaat
     2161 ttgcagaggc agacaaaaca gaaaatgaaa gatcagaata aatacagtca acgtggtgtt
     2221 tcggcagaca aagaagatgt gcatgcagca attaaaaatg ttgacaaagg tctttttcca
     2281 aaagcgtttt gtaaaatcat tcctgactat ataagtaacg acaaagatta ttgtttgctg
     2341 atgcatgctg atggtgcagg cacgaagtcg tcattagctt atgcttattg gaaggaaaca
     2401 ggagatattt ctgtctggaa aggaattgcg caagatgcgc tgattatgaa tattgacgac
     2461 cttctttgtg ccggtgctac agataatatt ttattaagct caacaatagg aagaaataaa
     2521 aatctgattt ccggtgatgt cattgcggct attattaatg gcactgaaca cttggttgct
     2581 gagctgaaaa attacggagt taatattaca accaccggag gtgagactgc agatgttggt
     2641 gacttggtgc gtaccattat tgttgacagc accgtaatgt gcagaatgaa aagaagtgaa
     2701 gttgttgata atgcaaatat tgttgccggt gacgttattg ttggctttgc ttctttcggg
     2761 aaagcaactt atgagactga atacaacagt ggaatgggta gtaatggcct cacttcggca
     2821 agacatgata tgttcaataa atcagttggc ataaaatacc ctgagacttt tgataattca
     2881 cttcctgaag atgtggttta taacggcaag tatgcattaa ccgataacat tatacttgat
     2941 gacgggtatg gaaaaattga aatgcatgca gccaaggcag tgctatcgcc tacgcgcact
     3001 tatgctccct tggttaagaa aatatttgaa tcgtatagaa aaaatattca cggcatgatt
     3061 cattgcagtg gaggaggaca aactaaggtg cttcattttg ttgataatct tcatatcatt
     3121 aaagacaatt tatttccgac tcctgctctt tttcgtcaca tacagcaagc agccggaacc
     3181 aattggatag aaatgtataa ggtatttaac atgggtcatc gcctcgaggt gtattgtcct
     3241 gtagccatag cagatgaatt aatttctatt gcggccgaat ttaaaattgg tgcaaaggtt
     3301 gtcggacgtg ttgaaaattg tgcgggtaaa aaacttacca tcaattcgcc atatggaaaa
     3361 ttcgagtact agttattttt ttgataacgg gcatggcgtt tactcagttc atcaataacg
     3421 ccactataaa ctgtgttcat cagttcagga tttgcaagat aaaagttcat gctgtgggaa
     3481 aatacagcag tatcaattct atgatgctca aaaatatatt tgtaataagc aggaaggtta
     3541 atgatgccta gtgaatcatt atatggatgc aaggtagcct ctgattcagc aagatgaatg
     3601 tcggcaagta tctgtatcat gctgtctttt gacagtgtac cttcaggaat tattacttct
     3661 ttatttttat gagtgcagga tacaatagat aaagtcagaa tagaaataag aaaaaagtat
     3721 tttttcactt gcatatatta atgagaagaa aataataatc gctgtccgtt ggtgcttaat
     3781 tggagatggc catcattgta aatgtgaaca ccgtttacaa aagtatgttt tactttcgat
     3841 tgaaatgttg taccttcaaa tggcgaccat ccacatttat agaggatgtt gcttttggca
     3901 acagtccaag gttcattcaa atttactaat gatacatcag cagcaaaacc ttcttggata
     3961 aaacctctgt ttttaatatt gaaacatata gccggtgaat gacacatttt atttgcaatc
     4021 tgttccaaag aaatttttcc ctgatgaaaa agctccagca tggcaacaag cgcatgttga
     4081 accaatggcc cacctgaagg cgcttttaga tagggcattt gtttttcgtc taaagtgtgt
     4141 ggtgcatgat cagttgcaat gacatcaatt ttatcgctga gcagtgcgtt taaaatagct
     4201 tctttgtcaa cttgtgtttt tattgcagga ttccatttaa taaaattacc ttttgaagca
     4261 taatcgttat catcaaacca aagatgatga atacatgcct ctgaggttat tcttttatct
     4321 ttaagtggaa cattgttaga gaatagttct gtctcgcgag ccgttgaaat atgaagaatg
     4381 tgcaaacggg tgttatactt ttttgcaaga ttcacagcaa aagaacttga caggtagcaa
     4441 gcttcttcac tgcggataag aggatgatgt gatgcattaa gtttatctcc gtaaagtgat
     4501 ttgtatttct ctgtattttg tcggattgtc tgttcgtctt cgcaatgtgt tgcaatgagc
     4561 attggcgatt gtgaaaatat tttatccaat gtagttggat tgtccacaag catatcacct
     4621 gttgaagatc ccataaaaat ttttacgcca caaacatttt ctctgtttgt tttaagaact
     4681 tcgtcaaggt tgttgttgcc tgcacccatg tagaaagaat aatttgccca cgattttttt
     4741 tgagcgagcc ggtatttttc ttctaataat tcttgtgtca atgtgtttgg tactgtatta
     4801 ggcatatcca taaacgaagt tatgcctcct gcaactgcag ctgccgattc ggaagcaata
     4861 tcgcctttat gtgtaagtcc gggttcacga aagtgcacct gatcatcaat aatccctgga
     4921 agaagtatta aaccatcacc ttttatttca tcaaccttat gttttacatt tatcgaaggg
     4981 tcaattcttt caatgatgcc attcttgaag taaatatcag aatcgaaaat tttgttgtcg
     5041 tttacaattc tgacattatg aataatggtt tgcatttgag tgaaaaaatt tctgctaagg
     5101 tacgaatcag catggtttac cagtgtttag attataaatt cagaagaatg tttttatgag
     5161 tttacatagt agagataaaa ttaattgcaa cattttttta tttttgcatg cttataaaaa
     5221 acaccgttcg gggtgtagcg cagcccggta gcgtatcagc atggggtgct ggtggtcgct
     5281 ggttcgaatc cagtcacccc gacagttttc atctcaaagc gttctgtaaa acagggcgct
     5341 ttttcttttc acacataatt tgtggatatt attcagcacc ttatgtaaat tacttatcac
     5401 agtcatttat ttttcagtcc gaaatttaac acatggcatt aagcagaatt tggtcgtata
     5461 tgattttatt tgctgtagca gtagcaggct atcgttacat gcaaggtgac aaaaatattt
     5521 ttaatgacat ggtggttgaa aaaaaattag ctgccggtga aagtttcagt aaagtatctt
     5581 attatgtcat tggtcaggga attgacagtg ttctcaaaga accaaaatta aaaaatgcca
     5641 attgggtaaa gacagataaa ttctcagatg ctcaggtaat aatcaccact gctgatttaa
     5701 agaataatct gcgcgagcaa ttaaataatc aacttgcaaa aatcagtttt gcgaatatta
     5761 acactcaaaa caaattcact gctgacaaat cgaaattgat ttattctgag gagttaatta
     5821 ctgacagtac attaaatata acaaacaatc tgcttcaggc taatgttgtt cttacatctg
     5881 aagtaaatac agattcgctt cgaaatgcat ggacagctat cagcaacaga caaaaaattg
     5941 ttggtagcaa tgaagtattt ctctggcttc ctaaaaacat tgatggtatt tttgaaacag
     6001 gaaaatttgc agtgactatt gccataggtc ttataggtac gctggcatta tttatgggct
     6061 tactcaatat tgctgaacgt gcaggtggca tcaacatcat cagccgatta ataagtccgt
     6121 ttttgtcgaa gctttttcct tcgttgccaa aaaatcatcc atcgtttgga cacatggtga
     6181 tgaacttttc tgctaatatg cttggcctcg acaatgcggc tacacctttt gggcttaagg
     6241 ctatgcagag tttgcaagaa attaatacca acaaagacag ggcaagcgat gcacagatta
     6301 tgtttctggc attacatgca tcagggttaa cactgattcc tgtttctata attgctgttc
     6361 gttctgctaa tggatcgcac aatcctaatg agatttttat tccgctcatg cttacaactt
     6421 ttgtttgtac tctcacagct atgattgtaa cctcgctcaa acaaaaaatt aatttgttcc
     6481 aacgtggatt gttgttacct gttgccggac tgagtttaac attaagtttg tttgtatggt
     6541 tcttaagcag cctgcgacca tttgcattag atcaattttc tgatgcattc agcaatggtg
     6601 ctattttatt ggtgtttttt ggaattattg caggaggact ttataaaaaa ataaatctct
     6661 ttgatgcatt cgttgatggt gcgaagggag gttttgaaac agcagttcgt attattcctt
     6721 acatggtagg aatgcttgtt gcggtgagta tgctcaggac aagtggtgcg tttgatttaa
     6781 tcattaatcc tgtaaaacat cttattgcaa tttctggcat ggacacacgt tgggcagaag
     6841 gtttaccgac agcattatta cgacctttca gtgcaggtgg tgcaagagga tttatgatag
     6901 atgccatgcg cacatttggt cctgattcgt ttattggtaa gttagtgagc gttttgcaag
     6961 gctcgagtga aacgacattt tatgttgttg ctttgtattt tggctctgtt tctgtaaaag
     7021 atacacgtta tgctattcct gttatgcttc tggccgacct ggccggagta atattctcaa
     7081 tcgtaatctg ctacttgtta tgggcataat ctaacagttt agttgttagc gagttacata
     7141 cataaaaatc cggtttaatt taagttgatt ttaaggccag aaataggggc ctactaatca
     7201 agttttttta tactttcgca acctcaaaaa ttttaaccgt agtattttat gagtcagata
     7261 gtttatgtag ttccggtgtt gggtttgttg gccctggttt atatgtttat aaaagcctcg
     7321 tgggtaggta agcaggatgc aggtgaaaag aacatgcaag aacttgcagg tcacattgca
     7381 gatggagcca tggcattttt aaaagcagaa tggaaaatat tatcctattt tgttgttata
     7441 gcaggattgc tattggctta ttcaggaaca ctacatggca cagaagaata tcctgttcat
     7501 tcaagcccga taatagctgt tgcctttgtg ataggtgcag ttttttctgc agtagcaggc
     7561 tatattggaa tgaacattgc cactaaagca aatgtccgta cagcacaggc agcaagaagc
     7621 agcctttcaa aagcattaaa agtttctttc accggaggtg cagtaatggg acttggtgtt
     7681 gcaggccttg cagtactagg attaggttcg ttatttattg tattctatca attgtttgtt
     7741 cccacaggtg cagcaattac cggtgttgaa atgaaaaaag ctattgaagt attagcaggt
     7801 ttttctctcg gtgcagaaag tattgcttta tttgctcgtg ttggcggagg tatttatact
     7861 aaagctgccg atgtaggtgc tgacttagtt ggaaaagttg aagccggtat tcctgaagat
     7921 gatccgcgta accccgctac aattgcagat aacgttggcg acaatgttgg tgatgttgcc
     7981 ggtatgggtg ccgacttatt cggttcttat gttgcaacta ttcttgctac aatggtatta
     8041 ggtcaggaga ttgacgcttc tgctgataag tttggcgggc tttcacctat tttattacca
     8101 atgcttattg caggaatggg aattattttt tcaattgtag gaacatggtt tgtgagaatt
     8161 aaaagtgaaa atgataatgt tcagaatgca ttgaacatgg gtaactggtc gtcaattgta
     8221 cttacagcaa ttgccagtta ttttgccgtg acttatttac ttcctgaaaa attagttatc
     8281 cgtggatttg agttcacacc tatgaatgtt tatttagcca tattggttgg tcttgtagtg
     8341 ggagcattaa tgagcatcat cacagaatat tatactgcta tcggaaagcg acctgtttta
     8401 tcaatcgtta aacagagctc aaccggtcat gccacaaaca ttattggtgg attggcagta
     8461 ggtatggaat caactgcagt gcctatgatt gttcttgcag gaggtatttt tctttcctat
     8521 tcattagcag gtctatatgg cgttgccata gcagcagcag gtatgatggc aacaacagct
     8581 atgcagttgg ctattgatgc ctttggtcct attgcagaca acgcaggcgg cattgccgaa
     8641 atgagtcagc ttccaaaaga agttcgtcag cgcacagata atcttgatgc tgtaggaaac
     8701 acaactgctg caacaggaaa aggttttgcc attgcatcgg cagcattgac atcattagca
     8761 ttgtttgcag cctttgttgg tgttgcaggc atttcgcata ttgacattta taaagccaac
     8821 gtgcttgcag gcttgtttgt tggtgccatg attcctttta tattttcttc gttggctatt
     8881 gctgccgtag gtcgtgctgc aatgagcatg gtagaagagg tgcgcagaca gtttcgcgaa
     8941 attccgggca tcatggagta caaagcaaag cctgagtacg aaaaatgtgt tgctatctct
     9001 acagaagctt ctatcagaga aatgattgct ccgggtgcat tagcattaat tgtacctgtt
     9061 gtagttggat ttattttcgg acccgaagtg cttggtggtg tgttagcagg tgtaactgtt
     9121 tccggtgtgt tgatgggaat gtttcagagc aatgccggtg gtgcatggga taatgcaaaa
     9181 aaatctttcg agaagggtgt tgaaataaat ggtcagacat attacaaagg ctctgagcca
     9241 cataaagcat ctgttaccgg tgacactgtt ggtgatccat ttaaagatac atcaggacca
     9301 tcaatgaata ttttgattaa actgatgagt attgtttctt tgattattgc accacacatt
     9361 gcatcaattg gtactcagga ttcttttaat aacaagagcg aagctaaaat tgttgctccg
     9421 gttgtaactt ctacagtaaa tagcacacct gaaatggatc ttgcagccat gacaggaaca
     9481 attagccttg acgtgagcca ctcctggatg cagtttggta tatcgcactt tctgacaaca
     9541 gcaaacggaa cattagcagt agatacaggt tatattaata tcactaatga cttggcaaca
     9601 agttcaattt ttgttcagtt gaatgtaaaa tctattgaca caaaaaatac aaagcgaaac
     9661 aatcatcttt taagctccga cttttttgat gctgaaaaat tcccttcaat agtttttaaa
     9721 tcaaacaaaa ttgaaaaagc cgcagagggc tcagcatttc gttatattgc taaaggtgag
     9781 ttaacgatta aaaatattac aaaacctgta gagcttagat ttaactacat gggtgcattg
     9841 ccaaatgaat atgcagctaa agatgcacat acttttgtgg gcgaggcaga tcttgacaga
     9901 attgactttg gtgtaggcga atcagggcct acagtaggta ataaagtaaa agctactttc
     9961 tctgttgaag gctacaaaat ttaaataaag agttagtgtg aattcagaaa ggctgtctta
    10021 aagggatggc cttttttatt ggcactaaaa aatcgttatt cgattaccat ccgcttgcaa
    10081 gcatatcggc aatatgcata aactgcaatg atgatccttc attgtctgca atacttttta
    10141 aatgcatcag acaggtatag tcagtagaga taactgtatt agctcctgtt tgtgttaact
    10201 ccagaatttt tctcttacca atatctactg aaatggcttc atacttttct gtaaatgatg
    10261 gagaatagcc gcagcaactg tctgtgcttt caatccattc aaggcctttt actttgctga
    10321 gcagggtgcg aacagaatct tttacagtga gttcattaat ggctttacag ctatcaagaa
    10381 ttacaacctt tccattaaac cgcgcaccaa catcggttac attgagtatg ttgactaaaa
    10441 aatctgtaaa ctcataaata tttttctgaa cacttttata ttcattgtgc aatgctgagt
    10501 tgtgaaacat ctgcgggtaa atatttttta tcattcccga acagtaagca ccgggacaaa
    10561 caatgtatcg ctcattctga aactctttaa tcaacttctc gccaacggct ttacattctt
    10621 tctgataacc ttcttcaaat gctgttctgc cgcaacatgt ttgttctaca ttatagttaa
    10681 cgccacatcc aagtttctca ataattttca caacattatt ggctgtttca ggaaaaacct
    10741 gatccatgta gcagggaata agtaaatcaa ctatcattct ttatttgcaa atatccgata
    10801 aaaaaatcag gtgttttgta atctgaaatt tctgatgcta agtaatgaaa aaagtcccac
    10861 tacaaaaaaa gtcatcaaaa ctaaaattga attacgcatg gaacctgtaa gttcttcaat
    10921 atatccgtaa gctactgttc ccaataccaa accaatttta tcacaaacat cataaaagct
    10981 gaaatacgag gcatggtcct gtgttttcgg tagcatttta ctataagtgg aacgcgatag
    11041 tgactgaatg ccgcccataa tcataccaac agcaaatgct aatacaaaaa actgcaccga
    11101 tgtagccaca aaatatgcag cccagcaaat gccaatccaa atggttactg caatcagcaa
    11161 ggctttaatg tttcctgcct ttgatgaaag aaatgcaaac agataggcac cggcaatggc
    11221 aacaaactga ataatgagaa cagtgatgat taattcatca tcgcgcatgt gcagttcctt
    11281 ggtgccaaac aaactggcaa catacataac ggtttgcaca cccatgttgt aaaagaagaa
    11341 tgaaatcaaa aatcgtttta gcattggcaa cgactgaagt tgtttaagca cttttattaa
    11401 ttcattaaaa ccttttgtca gtagttgttc atttgtgtta tgcgtactgg ttttttcagg
    11461 aagatattta aatgtgaaaa gtgaaaatcc aaaccaccaa acgcctgtca ttaaaaaaga
    11521 aattcgtgaa gccatgcctg agttggtaat tccaaaccat gatggattta atatcattgc
    11581 cagattaaaa atcagtaaaa gagagctgcc aatatagcct aatgcaaacc cctgtgcact
    11641 cactttatcc tgcatgtcgg cagttgcaat ttctggtaaa aaagcattgt agaagacaat
    11701 acttccggcc cagccaatgc tggcaagaat aaaagaaatg attcctatat cgagatttgc
    11761 aaggtcttta aaaaaataaa gtgatgcaca agatgtagag ccaagaaaac aaaacagcct
    11821 cataaaaaac tttttatttc ctttataatc tgcaatgcca ctaagcagcg gtgaaagaaa
    11881 tgctatcact aaaaaagaaa aagatagggc ataagagtaa agtgctgtgt ttgtgaaagt
    11941 gcgaccgaga aatgttacct gatcgctacc gttggcactg gtaatgctgt tgtagtaaat
    12001 aggaaaaata gcagtggtaa tacttagtga ataaactgag tttgcccagt cataaaatgt
    12061 ccaggcgcgt aaaactttag ggttgttttt ttccattaaa atcaatgttg atttattgcc
    12121 atataaaatt taatgcacca ttggtttgac ctccgttaaa caattgctgg ccagcataag
    12181 gaagttgatt agtcataagc ggagtaagat ttttttctat cactactgtt tccattaaat
    12241 catcagtttc cggactgaag ttgtagttga atactttaat aataattgac ttgtcgaact
    12301 gcgtaatggc aaagggtgta ataaaattaa aatttgctgt gccttgtggt gcattggtgc
    12361 tcactgttgt actgacataa gtatttccat tccaaatctg acagtaaata tcggctgcag
    12421 aaccatcact atcccatgtc gttccatctg atttatgaac aggccagcta tttagactta
    12481 gactgacaat gttagctttt gtgtaaggtg atgcgttgat gccttttatt gttgtagctg
    12541 ttttaccctc tgcagtttcg ccttttaaga caacattata aaatccataa tcaaatatgt
    12601 gcattacgct atcgccagaa acttctgtgc tgccatcggc aaaattccat ttatagttac
    12661 aaggaattgt tgattcagct ttaaaaataa aagtgtccgg atgtgttttg aagtcattat
    12721 aaacagtgat acctaaaaaa ggtgacggac tatcatcgga cttttttttg caacctgaga
    12781 ttaaaaaact ggctacaaag attggtaaca atatttgtga aagaaataat agccaattta
    12841 gttttttaaa tcctgtactg cggcaatgcc acagccgaaa ccccattatt tttttaagag
    12901 tagttttttc cattcttcag gaatgttttc gctgacagaa agacggtaat tgtagcaaac
    12961 taaaacagta agcccatctg ccagaataat tttattgtta tcagtagttt taaaaacttc
    13021 aaagccaagg tcaaaactct ttgttcctac acgagtgcac catgttctga cagataaatt
    13081 atcattcagc aatgcagggt gccggtagtt gatttgtgtc tgtgcaagaa taaatcctgt
    13141 ttcgcgccag ttgatgttta atggcagacg ttcactaaaa tcaattcttg ctttttcaat
    13201 atagcttaga taaacggcat tgtttacatg acccatccag tctatgtctg aaaagcggat
    13261 ttgaatgggg gtaattgttt ccgacatatt tattctatga tgatgcgttt agaaatcaat
    13321 tgcttgtcgg taataatatt gagcagatac attccttttg aaataccttc ggtatttaga
    13381 gttatgtaat catgcaacag agaatttgaa ggaatgatat ttcttccctg aacatcaaac
    13441 aaactgcaag taaaagtttt tccgggtatg ctgatattga tttgtccttt tgcaggattg
    13501 ggataaacgt caatatcagg catatcaaca gtgttggtgc ttaaaatatt ttcgcaaaga
    13561 atatcactta aaaaacgtgc aacaagagta tcaatcagcg catcatcatg aatgccgtgt
    13621 acattgccgt tgtagagtaa tgcctgatag tgctgacttg aaaaaccaag gttattcatt
    13681 ctgtcattta tcacacaaga gccaaaaaga tgcggatagt tttgagacac attcataggt
    13741 tgattccaaa gccccggcat atagccgcag ccaacagtgc catcgcctgt ctgatggtat
    13801 gagaaaagtg ccgggtctgt attggattca ataaatgatg tatctaacaa agcgccaaag
    13861 agatttactg ctgcaagcac attgctactt tgtccattaa gattcaacct tccgaaaata
    13921 gaacctaaat catttctttg aacattgcca aagagcgtag ttacatcacc tattgcccca
    13981 ccatttatcg gagcctcgct ttgtttgtca acataggtaa aatgcatggc ggtgatggag
    14041 cctgcactgg caccacccac aaatgcaaga ttaatattgc ttgagtcaat tgctgcacgt
    14101 gctttaagaa atcgcatggc tccatgtgca tcttgtacag cacggtatgc tgctctgata
    14161 acttcgcttg agtcgaatgt aaatggtgca ccaaaaaaca ggtttccata aaatcctaag
    14221 cgatatgaca tggttgcaga aacataacct ctttcagcat aagcctgtgc aataacatcc
    14281 atatcatttt tgtttccggt aaaaaaacct ccgccatgaa tccataaaat tatagggcgt
    14341 gctgtgttgt tgtcgcctat gggtttccag atgttcattt gaagattagc cactgtattg
    14401 ttataggcag tgtcgcttcc gtaacttata tctctttcaa cataggtttg gtagattctc
    14461 ggataataag ggtcaagaca ttgcgtaaag ctttgtccgt aagaaataac ggcaagcatt
    14521 aaaagtaaaa ttctattcat cataaaaagg ttattaaaat ctgttgacta aagtatggtt
    14581 aatggatggc atttgcaaag ggtgtttttt gagtgattgg gtaaagcgag ataatcaacc
    14641 acagctgata agggtaataa agttaattag ataatgagat aatttgataa tcagccacag
    14701 ctgacaagtt tgataattca gaattatagg ttctgcatag tttcattatc attatttcat
    14761 accgtaggcg cgagcgtttt ttccgctcgt gccgtttatt ttgtttgcat cttgcaaact
    14821 ataaaactta ttttgccggt gcatggcgaa aaacaaaaga tatttcctga cgggttatag
    14881 tttgttacat taaccacagt tggttggatt gatgttttta caagagccat ttactgcgat
    14941 gagatagtta tgaatttaaa ttactgcatt gacaagaaaa aactaaaagt gtttgcttat
    15001 tgcattatgc catcacacat tcatttaatt gcaaaaacgg aagacaatgt gaatgtaagt
    15061 gatgtgttac gcgactttaa gagttacaca tcaaagtaaa ttattaagat gattgaagaa
    15121 agcctcacgg aaagcaggca tgaatgattg ctttatatgt ttgagtttta tggcaaaaaa
    15181 aaatacgcac aatgttaaat atcattttag caacagaata atcatgcctt cgatttatac
    15241 agtaataaat ttattgatca aaaagtaaat tacatacaca acaatcctgt agaagcatga
    15301 atagttacag agccatattt ttatgtgtat agcagtgctg atcaatttac cgatttaaag
    15361 ctggctgaac tatgaagtcc gatagctatt ggacggccgg aggccccaag gataaaaggc
    15421 acgagcggaa aggacgctca cgactactta gagacctaat aaaaaataag aacctaagga
    15481 tttgtgaaat tcaattgata atgtattgta aatagcttcg gctttatcaa agttgttgtt
    15541 ttagaactct gaccaagcta aatccatttt ttcgctatac ttattagcaa tttgattctg
    15601 cgtttcagcc attgactttc ttttatctag gccccataaa tctatcacca ttgaaatgaa
    15661 tcatgtggtc aggcgtgtct gccagccata cttctgtttc ccaagcaata ttattggagt
    15721 gctttttaaa ttcagtgaaa tcaggaaatg ctgttacata aatttttccg gctttacagc
    15781 ctttcagaaa ttcttcaagt tcaacaattc gtttcggaga aacagggcca tgtgaagtaa
    15841 cggcttcaat caggaataac cagtttttct tttcgtcata aatcacaaca tctggcaatt
    15901 tactgtgctg gtcaatcggt attcctaatt ctttcaactt cttttcatca acgaacaaat
    15961 ccttttttgc tgtatcgcct aaatagagta tcgaaccacc attagcaaat cttgcagcaa
    16021 aattatgcac gattgcagcc tgaacttcgt tgtgctttcc tgacgaaagt ttaatcgttt
    16081 tgccattgct tagttttacc ggaatcagat tttgttttct ttctttttta tatcgtttcg
    16141 ctaattcccc ggcttctgat ttgaattttt caactgcttt cttccaatct ttagttccaa
    16201 aattttgaat agcttctaat gcttcattgg taattgcata atgagcattc ggactattta
    16261 ccggtagttt tggattgtcc ggattgtaat ctgcaatacg agcctgaacg aattgatgta
    16321 aaacctgcct tctgaatgtc tctcttgtat tcggtgcgta ttctttttta tgcacatcga
    16381 gaacgaaagc cataatacct tttgtaactt ttaaactttt gcgagtcgct ttactccaac
    16441 tattacgagg cttaattccg cagagtgcca ataacgtgta tgccgatatt tcattttgct
    16501 gtgctgttgg caaccctaat tctttgagta tcttttttgc ttcgtcaagt ttgctcatat
    16561 tgttgctaat gtaaattcaa attgttcatt tactattttg ttggctttat ctactgaaaa
    16621 atcattagaa agaataacgc tatttccaat ttcttttatt gtttcaagtg gaggaagggg
    16681 aatttccctg agttcggtag cgcttacatt cacgttgccg ttgaatattc tgaaatacga
    16741 atcgaataaa ctgctgttca gtaaagcaca aagcccaatc acctcatttc gctctaaatg
    16801 tcctttcgga cgatagatgt aatttacttt gttttctaca ccgatatatt ctgcttcaat
    16861 gaaattgcag aagtatgggg ctgcaaccaa tctgcttttg tcatccttag cactgaaacg
    16921 tctcaataga acatagtttt tattcggaat aagaatggat tttgattcat catgtactct
    16981 tacatattgt cctttttcgg gctttggcat gggccaatcc aaagccattt gctgtacatt
    17041 gtgaagccaa aacaatggag ctaataatac tgttccgttt tgatatgtat cgtgtatatg
    17101 atcttttgac ctgaatgcaa caacggggcc ggttgaaatc tgaatattgt atttattcag
    17161 gttgccgctc caatttttaa aaacattcaa aatagcttca tctgaatcac tcgttggtaa
    17221 gtacaatatt ttttcatttg agctcaggtc aatcaattcc ttttgtgcga aagatttgat
    17281 tattggagaa tcaatatcct ttaatccatg cgatgaataa acatgtactt tgtgctttgg
    17341 atttgttttt tctttacgcg ttccttttat gataacggtt tcctgcaata ccttatctct
    17401 gttgaatgtg tcttttcggg aaacaaacaa atgcacgttg tcaatttcaa ttattttgaa
    17461 aaaatactcc cggaataatt tgaagtaact gccagaagcg tagcttctcg gagttatgaa
    17521 tattagttgc ccattttctt ttagtagcct tgcggaaata gccataaaaa tggcgtaaat
    17581 attcggatgt ccattaacaa ctgcttttgc agcaatcgct cttttgtcgt caataggtag
    17641 tttgaagtag ggaggatttg aaacaataat atcgaacgga ttaattgtct gtgaaaacaa
    17701 gttgccatta tctgttaaac aacctgcgtt ttcaagaatg aaatcatctg tatgaataga
    17761 gaattttaat tcaatatttt tcgcatttaa ccaacttctt aaataatcta atgactcttt
    17821 tgtataagga attaaagctt tgtctgtttc gtacacaatc aagtcaatct cctgcaattt
    17881 gctattgttc tgaactatag attcgattaa tgcacaagtc agaatagccg ttccgcaacc
    17941 ggggtcaagt attctaagag aactttcgct tacttctgca aatgtcccca tcagtccggc
    18001 tatttcaata ggagtgaaaa attgtccgtt tgttttttta tgttcctgat ttaccgattg
    18061 ggcataattc accccaagcc ggtcagcgca atggctcggc aactcatcag caattttttc
    18121 tatgagtttt ttatctgcca tttttctttt tagcgatata gttaattttc ttttcggcca
    18181 cttgcatagc ttttagcgct acttcttcgt tttgcagttc gtaaacgagt ttgtcgctga
    18241 tgaacgcgat caaaaattca ttcaggtcaa ctttataaaa tttagccaga cgaataacct
    18301 gatcttttgt cggcattcgt tcaccacgtt caattttgct taaaagagcc tggtcaattt
    18361 ctatttccgc agcaacctgt cgaagcaata gtccgttttg ctctcttaat tctctgatta
    18421 tttctgctat atttttcaac ttgatatgat ttgacttgac atttaatgtc caaaaatagt
    18481 aaaatgattt ttgtagccaa agaataatta ttaacaacag cattaactag cgggcgggtg
    18541 aattttggtg cgtattgaaa attgcgggct tgtgcccttc agggattgag ggtgagcaga
    18601 ggagaaaaga attgtttaaa atacaaatat cctcaagcaa tcgttacttt tcgcagacct
    18661 aaacatgcca cacatcaaac aaagcctgtg aaattaataa ttctgaattc tacattctac
    18721 atagtttcat tatcattatt tcataactgt ataacaaaaa aggccatctc aaaaatattt
    18781 tttgagacag cctcctgaac caaactatca aatattttac ttcttcacca actgaatttc
    18841 tgcagcaatc ttcacttctt cgctaaccaa tacaccaccg gtttctaaag ctgtgttcca
    18901 gttcagtccc cagtctttac ggttaatttt tccggataat gtaaatccag ccttttcctg
    18961 accccatggg tctttcatca taccaccaaa ctctgcaatc aattttacag gtttagaaat
    19021 acctttcatt gtaagttggc cattaagtgc gtaagtgtcg tcaccggttt ttgaaaaatt
    19081 ctctgacttg aatgaaatag aaggatgttt ttctgcatca aaaaagtcac cgctctttaa
    19141 gtgtgtatca cgttgtgtgt cgcctgtggt aatgcttgat gcatccatac tgaaattgat
    19201 atttgcatta tcaaaattgc tgtcttcaga ttgtactgta ccttcaaact ttgcaaagtt
    19261 tccttttaca ttcgatatca tcagatgttt tactttgaat gtaatttcag agtgtgcctg
    19321 atctatgccc catgttgtct ttgtgagtgt tgtcataatt ttattttttt aaattgttaa
    19381 ttgtattgtt taattgagtt gcattggaat atccatcagt aatatttctg catcttcggt
    19441 tttagcgata aagtttattt cagaggtatt ccataaccct atggcatcgc gctcatgcaa
    19501 caaattgtgg tttatttcaa attcgccttt cactaccata gcatatacac cgttaccttc
    19561 tttttttatt ttatatgagg tgcttttgtt tttatcgaag ttgcccatac tgagccatgc
    19621 ctgttggtgt atccacaatc cctctttttc tgtatcgggt gaaacaatag tttgcagttt
    19681 attttttctg tcattgacat tgagtgatag ctgatcgtag cggggtttta cattttcttt
    19741 atcgggataa agccatatct gaaacaactt taccgcatct gttttacttg cattaaactc
    19801 actgtgtgta acacctgagc ctgcactcat cacctgcact tcgccatatt ttatgatgcc
    19861 atgattgttc atgttgtctt tatgttcaag cgcaccagct aaaggaatgg taataatttc
    19921 catgttgtcg tgtggatgtg tgccaaagcc cattcctggt tcaacatgat cgtcattgag
    19981 tacacgcaac acgccaaagt gcatttgatt gggattatag tagcttgcaa aactgaatga
    20041 atgttttgct tttaaccaac catggtctgc atttcctctg ttttctgctc tgtgaattac
    20101 tttattttcc atatctttaa aatttatggt acaaaagtag agcgacctta ttctttcagc
    20161 aataacctga attaataaag caacttaatg taggttaagt taatatgtgt gagtaagttt
    20221 tggtgtcagt ttttagtcat gcgtgcacgc atcttactga aaaattccgg tgtgacacca
    20281 atataggaag ctatatattt ctgtggtaaa caggaaacca aagcaggata cttcagacaa
    20341 aacttttcga atcgttcttc tgcactcaga gagagtttat cgagtattcg ctcttgctga
    20401 cttgccatgc tgttctcaat cagtattctg aaaaaacgtt caaacttggg gattttatca
    20461 aaaagtaaat cacggttttc ttttgtgagt acaacaattt cactttcatc aatggcgtca
    20521 ataaataaaa ttccaggccg gccagttatg ctactataga aatcgccaat ccaccatccc
    20581 ggtggtgcaa attgtaaaat atgttctgtt ccgcttttat caatattata agcacgcaaa
    20641 caaccagaat tgacaaagca tgatgcagta aacacttcgc ctgcctgcat aacaaattgc
    20701 ttgcgtctga ctatgcgggc acttagtaat gatgtaaata ttgcttcttc ttcttctgaa
    20761 agagaaatat atcttgaaat atttttcaga ataagattaa aaggtttgtt gctatctgtt
    20821 tttgccatgc tgctcaatat aatcaatcag ggcatgaata attttaatat gaatttcttg
    20881 tgttctgtcg gcaaagccgg aatgtggaac acgaatttca acatcacaat gattttttaa
    20941 tgctccgcca tctttacctg tcaatgcaac aacaataata ttttttgctt gcgccacttt
    21001 tgctgcttcc aaaacattgg cagagttgcc acttgtgctt atggctaata aaacatcacc
    21061 tttgttgcca actccttcaa caaatcttga aaaaacataa ttaaaaccat aatcattacc
    21121 cacacaactc atataggatg gatcagagat actgattgct gcaataggtt ttctgtcgtt
    21181 acgataacgc ccagccaatt cttcggcaaa gtgcatggca tcgcacatgc tgccaccatt
    21241 gccgcacgat ataattttgc ctcctgctgc gagagaggca gtcatcaatt caccggcttt
    21301 tgttacggct gcaatatttt tatcgaccga taaaaaagtt tcaagtactt tttttgcttc
    21361 ttcaaaatgc tgacgaataa tttctgacat atataagttt atatagcaca aagatagcag
    21421 gttatttgaa ctgaattcag aacaattgat tttaaaagta ttggttattg attgaaaaaa
    21481 tgattgtgat gtaaattgtt tttatttgca gtaattcctt tctatgcagg taaagaaaaa
    21541 gttgatttta tcattgcttt cggcagtgat gctttcgtta ggctggcccg agcgcggctt
    21601 aacaccactt atttttattg catgggtgcc attgctgctt ttggaagagt acagtcatca
    21661 gaaaaaagaa cagaaaaaat tcgggttgtt tggctggacg tatttaacca tgctcggatg
    21721 gaatgcttta acaacatggt gggtttgcaa tgccagtttt ggtggaggcc tgtttgctat
    21781 tttctgcaat tcattattta tgacatgtgt gttttatgct tttcatattg ttaaaaagag
    21841 aaaaggacat gtgtggggct atacagcatt aataattttt tgggttgctt ttgaatacct
    21901 gcatctgaac tgggagttaa gttggccatg gctgacattg ggtaatgtat ttgcaacaaa
    21961 accacaatgg attcaatggt atgaatatac aggagtgtta ggaggcacag tttgggtttt
    22021 gttggtgaat attttaatta caagaatttt tgttgcatca gcagataaat tgaaacgcag
    22081 cgtgctgtat gcaactgttg caatagcttt tccggggttg atttcattgc tgcgctacta
    22141 cacttataag gaaacggaaa atcctgttaa tgtaattgca acacaaccaa acattgaccc
    22201 ttatggtgag aaattttcgg ggatgagtaa cgaagagcaa ttaattcctg tattgcgttt
    22261 ggctaatctg gagttgaacg attcgtcaga tgtaatactt gcccctgaaa ctgcaattgc
    22321 caattccgtt tgggaagatg agttggagtt atatccggaa gtgaagttga tcagaaagta
    22381 tctccaggtt aacccacggg ctactttaat cattggtgca tcaacaaacc gtgcttatct
    22441 gaatggacaa aagccatctt caacagctag aaaatttaaa gatcaggatg gttattatga
    22501 ttcctataac acagctttac tttttgaaga tggaaagcct gcacagattt ttcataagtc
    22561 gaagttggtt ccgggtgtag agaaaatgcc ttatccaaaa attttcggct ttctcgaaaa
    22621 actttcaata gatatgggag gcactgcagg aagtctgggt attcaggatg agccaactgt
    22681 gtttactaca aaagataaaa tgattatagc tcctgttatt tgttatgaat caatttacgg
    22741 aggttatctt gccaagtatg tccgcaaagg tgcgcagtgg attgcaatta tcactaatga
    22801 tggttggtgg ggcaacactc cgggctaccg tcagcatttt aactatgcgc ggttacaggc
    22861 agtttctttg cgaagaagta taggccgttc ggcaaatacc ggaatcagcg gctttattaa
    22921 tcagcgtggt gatgcatttc aacaaactcc ttactggcat gatgccgttg tgcggcagac
    22981 catcaacaag aacgataaat taacattttt tgcacagtat ggcgattatc cgggtctgat
    23041 agcttcaata ttggtattgc cggttattgt tattacgttt ttcggaaaaa gaaaaaatca
    23101 gtaagttttt ttcggaattt tgccacataa attttaatgg aatggaaaat tttgatgtgg
    23161 caattattgg ctcaggaccc ggaggctatg tggcagcgat tcgctgtgca caactcggtt
    23221 ttaaaacagc gctggtagaa aagtatgata ctcttggcgg cacttgtctg aatgttggat
    23281 gtattccttc taaagctttg ctcgattcat cagagcatta ttttaatgct gtaaataaat
    23341 tcagtgagca tggcataaaa atcaaagata tcaaagccga tatggagcag atgattaagc
    23401 gcaaggctca ggttgtgaaa caaacttgcg atggcattag ctttctgatg aaaaaaaata
    23461 aagtaactgt attgacaggt catgcaacct ttgtggattc aaaacaattg gatgtagttg
    23521 atgctggtgg taagtcaaac agagtaggag caacaaattt tataatagca acagggtcta
    23581 aaccatcatc attacccgga atagagatag ataagaaacg tatcatcaca tcaacagaag
    23641 cgttagagct aaaaactatt cccggtcatt tagtagttat tggtggagga gtaattggtc
    23701 ttgaattggg ctcggtttat gcacgattag gagcaaaggt tactgttatt gaatatatga
    23761 atgctattat tccaacaatg gatcgtgcat taggaaaaga attacagaaa gtgttgagta
    23821 aggaaggatt tactttttta cttgggcata aagtacaatc ggtaaaatca tcagctaaac
    23881 aagttgaagt cgttggagaa agcagcagtg gtgaaaaggt taatatcata ggagattatt
    23941 gtctggtttc agtaggtcga aagccataca cagaaaaatt aggccttgac aaagcaggtg
    24001 ttgcacttga tgaaaggggt agaattatta cggatgaaaa attacaaacc aacgtgcctg
    24061 gcatttacgc cattggcgat gtaattaaag gtgctatgct tgcacataag gcagaagaag
    24121 aaggcgtgtt tgtggcagaa caactggcag gtcaacagcc gcacatcaat tacagactta
    24181 ttcccggagt agtttacaca tggcctgaag tagcatctgt tggttacaca gaagaagaac
    24241 ttaaagaatc gggagtgaaa tataaaacag gaaactttcc attcaaagcc agtggcagag
    24301 ccagagcaag tatggacacg gatggttttg tgaaggtgtt ggctgatagc actactgatg
    24361 aaattcttgg tgtacatatt attggcccac gtgcggcaga catgattgcc gaagctgttg
    24421 tggctatgga gtatcgtgca tcggcagaag acattgcacg tatttgtcat gcacatccaa
    24481 catttacaga gagttttaaa gaagcttgtc ttgcagctac tgcaaacaga gccatacaca
    24541 tctgattaaa cataataccc ctaacatata atttatgaat attcagataa aagaaattat
    24601 taccgtttca atgatattat tctcggtaat agacatcatt ggctctgtgc ctgtaataat
    24661 cggtatcaga aaaagtgtag gtaccattca ccccgaaaaa acatcgttgg tagcattgat
    24721 gattatgatt gtttttctct ttgtaggaga aaatattctg tcattgatag gacttgatgt
    24781 ctcgtctttt gctattgcag gctcattcat actttttttt cttgccttag aaatgattct
    24841 tggtgtaaag ctttataaag atgaaatgcc ggcatcggca agtattgttc caattgcatt
    24901 tccacttatt gcaggtagtg gcaccatgac tacactactt tctttacgca gtcagtatga
    24961 tatgcttacc attattattg gagtattact aaatatgatt tgggtttatc tctgtctgaa
    25021 gaatcttgga tttattgaaa ggcttcttgg gccaacagga atagcaatcg ttagaaaagt
    25081 atttggtttt attttgctag ctattgcagt aaaacttttc agaagtaatc tcggaatata
    25141 aactcatggc taaaaaaaaa ttctatgttg tttggaaagg ggtaatgccc ggagtttatc
    25201 attcgtggga tgattgtaag agacagatag atggttttgc aggagctttg tataaaagtt
    25261 ttgaaacaga aaatgcagca cttaccgcat tccaaaaagg attgtcgggg tttcagaaaa
    25321 aaagtgaatc acaacattta tttcaagtag aaaaatcgtc atcaaaacca ataaaagaaa
    25381 gtattgcagt tgatggtgca tggaatacta aaacattgga aagtgagtac agaggggtag
    25441 atgtaaaaac aggtaaacaa ctttttctta aaggccctta tgctgatggc acaaacaaca
    25501 ttatggagtt tttggctatt gtgcatgctt tggcttattg caaacgacat cacattaatc
    25561 tacccattta ttccgatagc atgacagctt tgaaatgggt aaagacgaaa aaagcaaata
    25621 caaaattagc ttcaacgggt cgaaatgagg aattgtttga attaattgac cgtgcagaag
    25681 aatggttgaa gaataatatt tatgcaaaca aactgctcaa gtgggaaacg caaatctggg
    25741 gtgaaaatcc tgcggatttt gggcgcaagt agttgaaaat tgatgctttt ttctgttccc
    25801 ctttaaattt atgcaaccaa acacagtttt ttttagaact gaaaaagctt gaatagcatt
    25861 tgggggtatt caaaaacaat aagttgtaag caaaaactga tttatatcat attgttactt
    25921 aattgctgtc atataataac tactttaggc ggagtaggtt tgtgaccgtt ttttatgagt
    25981 gtacttatta tacttattgg agctagcctg gtggtggcag caggcttctt ggtttctttt
    26041 ttttgggccg ttcgcagcgg gcagtatgat gatgattact cgccttctgt gcgcatactt
    26101 tttgacaatg acaaaacttt aacatcagat aaatcaaatt caaacataac agataactaa
    26161 aattatgaca atagaaaagt ttcgctacga caacaagatt gttgccatgt ttgcttgggc
    26221 tacagtaata tgggctattg tgggcatgct tgtaggcctg ttagccgctt tccaacttcc
    26281 ttttcctgca ttaaattttg atgcacacac aacttttggt cgtatcagac cgctacacac
    26341 caatgcaatc atttttgctt ttgtgggtaa tggaatgttt atgggaattt attattcgtt
    26401 gcaacgttta ctcaaagcaa gaatgtacag tgatgtgtta agctatattc acttttgggg
    26461 ttggcaatta atcattgtag gtgcagcaat tacccttcca ttaggaatta cagccagcaa
    26521 agaatatgca gaattagaat ggccacttga tattgcaatt gccatagttt gggtaatatt
    26581 tggcataaac atgtttggca ctttagttcg cagacgtgaa cggcatttgt atgtagctat
    26641 atggttttat atagcaacag ttgttactgt tgctgtgttg cacattgtaa actctcttgc
    26701 tattcctgta acattattta aaagctactc actttatgct ggagtacagg atgcattagt
    26761 gcaatggtgg tatggacaca atgcagttgc attttttctt acaacacctt atctgggatt
    26821 gatgtattat tttcttccta aagctgctaa ccgtcccgta tattcctatc gtctttcaat
    26881 cattcacttt tggtcattaa tatttcttta tatatgggca ggaccacacc acttgcttta
    26941 tactgcattg cccgattggg cacaatcctt aggtgttgtg ttttcaataa tgttacttgc
    27001 tccatcatgg ggtggtatga taaacggatt gctgacactg cgcggtgcat gggacagagt
    27061 acgcgataat ccggaactta aattttatgt tgttgcagta actgcttatg gtatggcaac
    27121 attcgaaggg cctatgcttt ctttaaaatc tgtcaatgcc atcagtcact ttaccgattg
    27181 gacaattgca cacgtacaca ttggcgcact tggatggaac ggatttctga ctttcggaat
    27241 tctttattgg ctgattccaa aattattcaa cacaagttta tattctaaaa aattagcggg
    27301 aatacatttc tggcttggga cacttggcat tgtcttctat gcaattccac tttactgggc
    27361 aggtattaca caacatttca tgtggaaaga atttactgaa gatggtgtgt tgcgttatcc
    27421 aaatttcctt gaaacagtag tgcaggtggt gccaatgtat aaactacgtg ctttaggcgg
    27481 atcactttat ctgcttggtt ctttggtgat ggtttataac cttattaaaa ctgcacgtgc
    27541 tggaaaaatg gttgctaatg aagatgcaga agcaccggca ttagaaaaaa attatgttgc
    27601 tcatgatgac cactggcatc gttggataga acgcagacct gctcagatgc ttatcggaag
    27661 tcttattgta attctcatag gaggaatggt tgaaattatt ccaactttta tggtgaagtc
    27721 aaacgtacca acaatagcaa gcgttaaacc atacacacca cttgaattac agggacgtga
    27781 tatttatgtg cgagaaggat gttatacatg ccactcacag atggtgcgac cgttccgcta
    27841 tgaaactgaa cgctatggtg aatattccaa gcctggtgaa tttgtctatg atcatccgtt
    27901 tcaatggggt agtaagcgaa ccggccctga cctgcatcgt gaaggaggga aatatccaaa
    27961 ctcatggcac tacaatcaca tgctcgatcc gcaaaccatg tcacccggaa gtattatgcc
    28021 atcctatgca tttatgattg atgatgattt agataccacc actactgcag caaaaattcg
    28081 tgctatgcaa aagttaggtg tgccctatcc tgaaggttat gatgcacagg caaacaaaga
    28141 tctgatgaag caggcaaaca gtattgcatc taatcttgaa aaagatggaa tcaaagtaat
    28201 gggtaataaa gaaattgtag ctttaatagc atacctgcaa cgattaggtg ttgatatcaa
    28261 agcagaaaaa actacagcct cagttaatta aaaataaaga agaatatgta taaagaagta
    28321 ttatcaacaa taaaggatat cagtatctat cctgtatttt ccttcgttat tttctttgtt
    28381 ttttttaccc ttgttggtgt ttgggttttg aaatcaaaga aagaagactt tgagtctata
    28441 agtaagctgc cactagaaga aacaaacgat taaaatctga agtaatgaaa aagataatta
    28501 atttaatatg tttgataatg ttcatctcat tatctgcatg ggccggtgat acacaggcaa
    28561 cagaaacaac aaaagttgca gccgctcagc ctccggattg gttaatgaat gcaaatactt
    28621 atctcattgc atttgtagta attattttac ttgccacaat attggcatta tatagaagca
    28681 gtcttaaact actgaaaaaa ataacatcgt tttgctttat aatgtttgta tcaggttctg
    28741 catttgcagc agaagaagca gcacctgcag aagcaacaaa ggttacagct atacaacctc
    28801 cggactggct attgcataca aatacttatc tgattgcatt tatcgttttt attttagttg
    28861 tgacgatagt tgtgttgttc aaaaccaaca tgaaacttat caggatgatt gcaccacatg
    28921 catttgatga tgagaagcaa acagcagaag caggagcagt ttcaggagtg aaaaaccgtg
    28981 aaacatttat gcgtaaagta tatctgcgct tagtggatag cgttcctgtt gctaaagaac
    29041 aggatattct tttagatcat gattatgacg gcattcagga acttgacaac aacctgccgc
    29101 catggtggaa atggggattt tatataacca ttattgctgc tgtgatttat atgttggcat
    29161 atcatgttac cggcacagga aaattgcaag ccgaagaata tgcagatgaa cttgctgttg
    29221 cagcaaaaca gaaggaagaa cgaatgaaaa attcagcaga gaatattaca gaagaaaatg
    29281 tgactgtgtt ggttgatgca gcagaaattg caaagggaaa agatatttat gaaaagaatt
    29341 gctcagcttg tcacatggct gatggtggcg gaatggttgg accaaacctt acggatagtt
    29401 attggctgca tggcggaggc gtaaagaata tttttaaaac cattacttat ggtgttcctg
    29461 caaagggaat gattagctgg caggcacaac tttcaccaaa acaaattcag gaagttacct
    29521 cttttattat gacacttcat gggacaaagc ctgctgcgcc taaagagcca cagggtgatg
    29581 agtggaaaga tgaacctgct gcaggtgcaa ctgcaacaga cagtgctgct gttgcaacta
    29641 ctgcagaggc agtaaaaaaa tagtattaaa tctctttttt atggtggaga ataaaatgga
    29701 gcatattgat gagtcgttca gagacagcat agccactgtt actgaagatg gcaagcgtgc
    29761 atggatttat cctaaaaaac ccaaaggcag atattataat gcacgaacat gggtaagcat
    29821 tgtgtttctg attattttct ttgcattacc gttgataaag attaatggcc atccattttt
    29881 tctgctcaat gttgtagagc tcaagtttgt attgttcgga attgcttttt ttccacagga
    29941 cttttatttg tttgtattgg ccatgctcac ctttgtggtg tttattgcat tgtttacagc
    30001 tttgttcgga aggctttggt gtggatggac ttgccctcaa actgttttta tggaaatggt
    30061 gtttcgcaaa atcgagtatt taatcgaagg cgattttatg aaacagaaag cactcgataa
    30121 acgagcctgg gatgcagaaa agatcagaat caagacaaca aaacatcttg tattcttttt
    30181 cattgccttt cttatctcca atactttcct gatgtatatt atcggatttg atgaatggaa
    30241 aaaaatcatt accgataatc cttttgacca cattggcgga ttgtttgcaa cagtaatttt
    30301 tgcaggagta ttttatggcg tgtatgcacg ctttcgtgaa caggtttgta ttgttgtttg
    30361 cccttatggc cgtttacagg gtgtaatgct tgaccccaac actgtagtaa ttgcttatga
    30421 ctataaaaga ggtgaaccaa gaggaaaact tagcaaagac aatgaagaac atggcgattg
    30481 tattgactgc aatcaatgtg ttcaggtttg ccctacaggt attgatatcc gcaatggcac
    30541 acagcttgaa tgtgtaaact gcactgcatg tatggatgcc tgtgatgaca ttatggataa
    30601 aatccagaag ccacgtggac tcatcagata tacgagtgag aatgctatta agaataatgc
    30661 taagtttaaa atcaattcgc gcattatcgg ctattgtgtg gtgctgttag ttttgttctc
    30721 ggcacttgta gttttattag ccagacgatc aaatattgaa acaacaattt tacgtgcatc
    30781 gggattattg tatcaaaaac cggatgaaag ccatatcagc aatctttata acattgcctt
    30841 gataaataaa acttttggta atatgccaat tgaattaaaa attgttgaac caaagggaga
    30901 aatcaaatgg gttggtaatg gtattacaca tcttaaagaa caatctgtgg cgcagagtac
    30961 tttctttctg attttgccta agtctgaaat taaaaagaca aaaacaaaag tagtttttga
    31021 aatctggtca gaaggagaaa aaatagatcg ctatgaaact tcattcttag gcccaaataa
    31081 ttaattgaaa tgaaattcaa ctggggacat ggtattgcat tggttttaat aatttttgcc
    31141 tcatcaatat tggtatttgt ttatgttgct tccaaacaaa aaaacgaatt ggtgacagat
    31201 aaatactacg agaaagcagt ggcttatcag caaagaattg atgcagaaaa aaatgcatta
    31261 aacgaaaatg ctgatgttaa gtataatatt aattccggtc agctaatgtt gacattaggc
    31321 agaaatatac caaatgcaaa tggtactatt cagttttata agcctgatga tgcaacaaag
    31381 gattttaata cttcatttca ggttaacgat acaggatttt atagtgccac tattcctcaa
    31441 ttaactcatg gtcaatggaa agttaatgtt agctggaatg ttgacagtaa agagtatttt
    31501 tacgagaaga aaatatttat tccataatga acgaatacac tttctattat gcagctttgc
    31561 tactgggttt tcttggtagc tttcactgtg taggaatgtg tggccctatt gccttaatgc
    31621 ttccggcaaa taagttagat aatcgcagag ctgcattttt tggcagactg ttttacaaca
    31681 tcggcagaat gataacctat atgtttattg gaatgttgtt tggattgctg ggtgctgctt
    31741 ttgttttaaa tggatttcag aaacaactga gcattattac aggaattatt ttgttgttag
    31801 ttgcaatatt tactttaatc ccttcaacca gaaaaaaagt tcttagtgta tcatcaaaaa
    31861 tgaatattcc tttcagagga gtgttaagaa aacttttttc aggtaaaaca gccttgcatc
    31921 attttggtat tggtgtcatt aatgggtttc ttccatgtgg atttgtatat gtggcaacgg
    31981 caggagccat caattcaggc agtgttttaa acagtgttct gtatatggca ttgtttggtt
    32041 tgggaacttt tccaattatg cttcttgttt cctatgcagc atcgtggtgt agtttttcat
    32101 tctctcgtta tgtaaccaaa gccacacctt ggatatctgc tgcattagca ttgtttctta
    32161 tttacagagg agcttttatg aatattaatg ccgattgtca taaacattca catccaccaa
    32221 caatggtaga atgtagtaag cctgaaacac tttaaaattt tattttatag gattgttcaa
    32281 ttgccgatat tcggtggctt aatgatattt gtatattcgc aacgctacat taaatcgaaa
    32341 aggtaaatga gtcataaatt attagatgcc ttgatgcacc tttttgccat tctggcaaat
    32401 gccgaaaaat tttctctcga aggcagaagt atagttgagt cttttttgcg acagcaaatc
    32461 agccgtgctt atgttttaaa gtaccttgga ttgtttgacg agtatcttga aaatatcaga
    32521 ggtaaggcaa cagaaggaaa gttgaaaaaa cgactttcag taaactcggt taaagtgctg
    32581 cgcatttgta cagacattaa tgcagaactt gatacccgcc aaaagcatat tgttttaatg
    32641 cggctgattg aattcgtttt tacctctgaa aaaattattg acagtcagga aaaagagttt
    32701 ctttatacag ttgctgaggt ttttcatatt gcacccgatg atgttcagtt atgcattgaa
    32761 ttggcttgta ataatgcaga agagaagtat tcagatactc ctaaatattt agtgttgtct
    32821 tcacaagatg ataacagcag tttgaatact aaaactcaaa ttatatcagg gttaaatggt
    32881 gttttccggt ttttatttct taaaaatgcc ggattgcttt ttgtgcgata tcacggtgat
    32941 gaatctttta cattgaatgg actaccactc aatgacgatt tatgttatgt tttttctgca
    33001 ggctcagtca tcagaggttc aaaggtaaac acaatctatt acacagatat tatcagactg
    33061 ttttcaaatt ttaagcaaca gagtttattg cattttgatg tacaacatgt gtattacgat
    33121 ttttcaaatg gtaaacgcgg gcttaatgat gttgccttta gtgcaacatc aggaaatctt
    33181 gttggtatta tgggtggaag tggcgcagga aaatctacat tactcaatat cttaaatgga
    33241 aacaatagac caacacaagg aaaagttact gttaatggaa tagatctgca tcgggagtca
    33301 catctgcttg agggtatgat aggctatgtg ccgcaagacg atttgctgat agaagagtta
    33361 acagtctatc agaatttatt ttataatgca aaactttgtt ttggaaattt aaccgatagt
    33421 gaaattgatg aacgtgtaaa ggcactttta ctaaatgtcg gcttaagcga agcaagtcat
    33481 ctgcgtgttg gcaatgcact cagcacatct atcagtggtg gtcagcgcaa gcgactcaac
    33541 attgcattag agttaataag agcacctgaa gtactttttg ttgatgaacc cacttcagga
    33601 ctttcttctc tcgactcaga aaatgtgatg gacttgttaa agcaacttgc caatcacggc
    33661 aaacttgttt ttgtagttat acatcagcca tcgagcgata tttttaaaat gttcgataaa
    33721 ctttttgttc tcgatattgg tggctaccct gcatacttcg gtaatcctgt tgactcactt
    33781 ctttatttta aacaacgtgc atcttttgct gatgccgatt atagcgaatg tggcacatgc
    33841 ggcaatatta accctgaaca gattttttca attctggaat cgaaagtaat ggatgagttt
    33901 ggctttccta cacaacaacg aaagctaact cctgcagatt ggtatcaact gtttaaggaa
    33961 cataatagta attctagtaa atcgaatcag cgacaacccg ttcagggtag tgcaaatcaa
    34021 aagcccggtt ggttgcagca actaaaggtt tatatgacac gcgatatgct ttctaagttt
    34081 aaaaataagc agtacatgct tattactttt cttgaagcac cggtgctggc cattattctt
    34141 gcatggtttc tcaggtattt taatcctgat ggcagttatg tttttagcga gaataaaaac
    34201 cttgtagcat ttttattcat ggcagttata gttgccattt ttatgggact cactgtaagt
    34261 gccgaggaga ttatccgcga cagaaagatg ttgaagcgcg aaaagtttct taatctcagc
    34321 agaagcagtt atctgtattc aaagatgatg gtgatgtttt taatttctgc tatacagaca
    34381 ttttcttttg tcattatcgg taatcttatt tttggaattc aaagcatgac ctttgattat
    34441 tggataatgc ttttctccgt ttcgtgcttt gccaatatgc ttggcctgaa tgtttcatcc
    34501 atgttcgaca gtgctgttac aatttatatc ctcattccat ttctgattat tccgcaaatt
    34561 attttaagtg gtgtgatggt aaagtttgaa gacctgaacc cgcacctgac ttcgcaaaga
    34621 catgtgccgt taataggtga attgatggct tcgcgatggg cttttgaagc attagcagta
    34681 aatcaattca gtaataatcc ttatcaaaaa tatttcttta atgtggacag acaaatgagc
    34741 gaagctactt acaagaaaga tttctggctg cagaagttga atgatattgc cgaaggtgtt
    34801 tctaaaaaca aatctaaaaa tccgaaagta gatgttgagc ttattactaa tgaattgcac
    34861 aaagaagctt tagttaataa aaattttaaa actgcaattg attttacaaa ggcaaatgca
    34921 tcagaaattg catcacaaat taaacctctg acagatatac ttcgtaatta ctacatagaa
    34981 aagtataaca atgcatctgc tgccaaggat agttttgtaa agaaagaact gaagcattca
    35041 tctgtagcga tgttgaacaa gcaaaaagaa aaatacacga ataatgcttt gaatgatctt
    35101 gttttaaata aaaatgactt taccattctt gttcaggatg gcaatagtat tgtgcagcga
    35161 tttcagcctg tctatatgca acccgaccca acaacatggt ttcggtctcc attgtatgtt
    35221 tatgagaaaa atttctttgg aatgaaaatt catacatggt atgtaaacct ttttataatt
    35281 tggttgatga gctttatact gatgacaaca ctctatttcg atagtttcag aaaactgatt
    35341 cattgggtgg gacacagtag caaacgatga ttttttctta actgtttttt cagaatattg
    35401 atataaaatt aaatctctga aggagacaat aaaaaactgc tattaaatca tttttttgtt
    35461 gaatgattta atagcagtgc aattttaatt actattttat taagaaagaa gtgttatccg
    35521 cacttactgt gtccgcagct tttgcattta atacaaccct cttcaaaata caatccttcc
    35581 ttgtcgccac actctgcaca caccttatca ttaacttttg taccatcagg aataaattgt
    35641 ttcaatgcac gcattacacc atttttccac gtgttaatgc tgtcggaata aagatgcaaa
    35701 ttttccacca ggttaactac ttgcggtatc ggcattccat gtcgcaatac accgctgata
    35761 agtttggcat agttccagaa ttctttatcg aacgagcgcg atagcccttc aatagttacc
    35821 ttaaatcctt ctttgtcatt aaactgaaaa tcgtatctcg atttgccatg tccattttta
    35881 ctcttgataa tccatcctga agaaacccat gcaggaatac tgaatgcatc ttctgcctta
    35941 ccggtaaaaa tttcataagg tttattgtct aatataccga tgtatgcaat ccatttttca
    36001 tctgcattca taaagcgtat aacttttgca gcaagaattt caggtcgttt tggtgcactc
    36061 gattctgtta ttacaggttc agcttttttc tcttcttttt ttaccaacac tccactgcgc
    36121 gaaccttctc tgtaaacagt aatacctttt aatccggcac tccatgcctg catataaatt
    36181 tcgccaacag tttctgctga aacattttca ggtaaattaa tagttgagct gatagagtgt
    36241 gtagtatatt tctgtactac tgcctgcatt ttaattctct ttttccagtc aatttcattg
    36301 gctgtgcttc ctgcataagg acttttactg tagtcggtgc tgttgtttgc agtcatccat
    36361 ttttttaatc cgtgatgata cacttcaaac tcctgccagg catcaccgga ctgatctaca
    36421 aaacttgttt tagccttgct gtcttcggca ttaacttttc ttctgcgttt gtaactgagc
    36481 ataaacacag gctcaatacc cgatgatgtt tgtgataaca gacttaatgt tcctgtagga
    36541 gcaactgtgc tgatagaaat atttcttctt ccgtacttca tcattctgtt gtaaatatcc
    36601 ggcaattctg ctttcatcat atttacaaac tccgactgat cttctattgc agtatcaaaa
    36661 ccttcaaacg ttccacgttc tatggccata tcaatacttg aatcaaattc tgcacgacat
    36721 ttttctttca tcatcatttc tacaatgcta agtgcattgt cggagtcaaa ggcaatgttc
    36781 atggcagcaa gtgcatcggc taaagctgta aatccaagtc ctgtacgtct tcctttttta
    36841 cctgcttcat acaatgttat ccatgtacgc atttctactt ctttaatgta gtcaggctca
    36901 gggtctgatt ctattttctg aagaatacgt tcaatagcct ctaactctaa atctaccaaa
    36961 ccatccatga gtctttgtga ctcataagtt acttcataaa aacgtgtgaa gttaaactta
    37021 gccttttgtg taaatggatg atctacaaaa ctcaataagt tgattgcaat taatctgcaa
    37081 ctgtcgccac cctgcattgc aatttcagag caagggtttg ttgacacgtt tttaaatccc
    37141 ggataaacgg aagatgtaga ataatgatgt tgtctgtccc agaaaataat tcccggttcg
    37201 gcagtacgat gcgcacaggc aatgatggtt ttccataatt cgcgggcatt aacttttttc
    37261 tgaatataag gatcagtgga atcaacaggg aatctcaggt taaaatcagt attctcatta
    37321 acggcaacca taaactcatc cgataatttc actgaaatat tggcacctgt aactttactc
    37381 aggtcttgtt ttacggtaat gaatttttct acatcaggat gagcaatatc tagtgtaagc
    37441 atcaaggcac ctctgcgtcc gagttgagca acttcgcgcg tggtgtttga aaaacgctcc
    37501 ataaaagata cagcacctgt agtagtacct gctgcattgg aaacacgcat accttcaggt
    37561 cttatagtag aaaggtctaa gcccgcacca catctgcgct tatataattg tgcaagttgc
    37621 tggtcggcaa aaaatattcc accataagaa tcgtgtaatt caggaagaac aatacaattt
    37681 gataatgatg caatcataaa aggattgccc agagcagaca ttacactgcc ttgtggaata
    37741 acataacgaa agcgatcgaa taacgctgtg attttttcag tagttagttg ctcgcgtttt
    37801 tttccgtatt ccgacagatg cgaagttctg ctgccatcgt tcagcagatt atattttgct
    37861 tcaactcttg caaattcacg agccatacgc aaatgcatat catgtggtgt tcgttctaca
    37921 aatttccctt ctttgttttt catggcgtat ttattcatcc atgttgtagc agcaagctca
    37981 tcgccttcaa aatattctag acttgctttc aatacgctgt catattcgta ggtggtcatc
    38041 tgactctcgg ttgtaatggt ttcaatcaac atattttata tttcttattt gtttaaggca
    38101 agcaataata tagacaaaaa aaattctttc ataaggattt gactaatatt tatcagacgt
    38161 tgttaacaac ccatgttcac tatttttatt atcaacaaag tacatcaaac aaagcgtttg
    38221 aattataggc aattggtact tttgctaaca cacaagtaaa aaacaaatga ttataatcat
    38281 tagcaaaatt taatctaatc attaagcaat agaaaatcta tttggttctt cgcatctttc
    38341 aaattctaat atgaaattca agacaattat cgagcctttc agaattaaaa tggtagaacc
    38401 cattcacctc agcacagagg aacaaagagt aggttatctg aagcaggcac attacaatcc
    38461 atttctactt aacagcaatc aggtacttat agattttctg actgacagtg gtacttctgc
    38521 catgagttct gaccaatggt cggcaatgat gaatggcgat gaatcgtatg caggtgcaag
    38581 tagttggctg cgttttgaaa aagaagtgca aagccttacg ggaatgcctt ttgtgttacc
    38641 tacacatcag ggtagggcag cagagagaat tctttatgga aagcttggtg gcaagggtaa
    38701 aacgtttatc agtaatacac actttgatac tacacgtgcc aatattgagt ttagcggtgc
    38761 cgaagcaata gacattcctg tgaaggaagg gcttgacttt gcatcagatt ttccttttaa
    38821 aggaaatatg gatatagttt tacttgaaaa aactattgca gaaaaaggtg cagctaatat
    38881 tggtgcagta atattaaccg ttaccaacaa tagtggagga ggacaacctg tgagcatgga
    38941 gaatgccaag gctgttgcag atatttgtcg taaaaacaaa gtgttgtttg ttattgactg
    39001 ctgccgtgtt gctgagaaca gttattatat tcatcataac gaagcgggat atgaaaataa
    39061 aacctacaga cagattgcag aagaaatgat gtgtttgggt gatgctgctg ttatgagtgc
    39121 aaagaaagat gcattagtaa atatgggtgg atttttagca gtgaaagatg ctgatttggc
    39181 tgaatcgtgt aaacaattgt tgattataac tgaaggtttt gccacctatg gcggacttgc
    39241 cggacgcgat atggaagcca tagcagttgg attgcaggaa gtgtttgatc cgggttattt
    39301 gaattatcgt attgtaagca caagatattt gggcgagaat attaaatcca aaggagtgaa
    39361 tattatttat cctgtcggtg ggcatgctgt ttatgttgat gccaaaagtt tatatcctca
    39421 cataccacca catgaatatc cgggacaagc cttggtatgt gaattgtacc gtattggggg
    39481 tttacgcagt gttgaaatag gatctgtaat gttcggaaag tatgattcaa atggtaagct
    39541 gattccggca cctatggaat tggtgcggct tgctgttcca agaagagttt atacacaaag
    39601 tcacatagac tatgtgattg aaatttttga tgaaatagtg cgtacaaaag ataaggttaa
    39661 aggactaaag attgttaaag agccacaatt cctacggcat tttacagcgc attttgagcc
    39721 actgtaattt ttgtgattag caattgaaca ttgcttaatg cggaaagcag cattatcgaa
    39781 aagttgaagt tgtaaatagc atttaccagc caccggtggc gccaccacct ccggtgctgc
    39841 cgccaccgaa gcctccaaat cctcctccgc tactgccacc gccccaactg ctgccaccgc
    39901 ctccccaaac tccaccacct cctgatgagt tacgtctgtt actcgctgca ttcaaaagca
    39961 tccaggctgt ccagaaactt aaattgttga gctgagcata gcgcctgaca gaaaaaactt
    40021 tcatcagcat tactacaccg aaaattaaaa acacaaaaag tatgggtgcc catgcaggtg
    40081 aattgttctt tgcatttttg ccatcagaaa catattcgcc ttttgttcgc tgcataattt
    40141 ctgttactgc agcatcaagt ccgttgtaat actgttgtga tttgaatgca ggcttcagcg
    40201 taagctcaat aattctttta caaatagcgt caggtaatgc tccttcaaca ccgtaacctg
    40261 taatgatggt catgcgcctg tcttccatag cagctaaaat cagcacacca ttatctttat
    40321 tcttctgacc aattttccaa tctctgaaaa gagctatagc ataatcatcc acaggatatt
    40381 caccggtact gcgaatcagt acaatagcaa tttgtgtaga agtgctgtcg ttaaattgta
    40441 caagtttttg ctctaaaaga ttttcatcag catcattcaa aactcctgca tagtcgttga
    40501 ctaagcgtgg tggattactt tgaggtggaa tttgttgtgc attaccaatt gttgcaatct
    40561 gaagcaacaa taacatccat aatataagag gacgtacctg ttttttaatc gctaaaaacc
    40621 acttcattgg aaagttcgtc tgaatcattc ttagcaacag gaaaatactt ttttaatttc
    40681 tgacctgttt cttcgatagc atgcaacaag ccaccggtat aatcttttct cttaaagaat
    40741 ggaatcattt cctctttaac cttatcccaa aaaacctgac ttacatattc gtgaatacca
    40801 ctatcaccaa taatggcaaa ctgtctgttt tcaatggcta cataaataag cacgccattg
    40861 tgaagtttgg tttcatgcat cttcaggttt tcaaaaactt cggctgcacg gtcaataatt
    40921 tcactcttta ccttgctgtc aataaaaatt ctgatttctc cggatgtatg tttttctgct
    40981 cttgctattg catttttcaa caacaattgt tgttcatcag aaaaataatc ggcagcgttg
    41041 gccataatta aaacttaacc tcaggggctt tattagcgcc tttatcagat tgaaaataac
    41101 cttttggtga aaacccaaac atgccggcaa gcaatgtttg aggaaactta cgtatgtagg
    41161 tgttaaactg ttgtgcagcc tcgttaaaat ctttacgtgc cacagcaatt cttctttctg
    41221 ttccttcaag tgttgcctgc aattcaagaa agttctgatt ggctttcagt accggataat
    41281 tttcagtcac cataagtaag cgacctaatg ctgtgcttaa ctgcccttgt gcttcctgaa
    41341 acttctgaat attttcaggt gtaagtttgt caggatcaac ttttacttgt gttgccgatg
    41401 cacgtgcttc aacaactgct gttaaagtgc ctttttcaaa ttctgcagca cctttaactg
    41461 tatttactaa gtttggaata aggtccattc gttgttggta ttggttctct acctgtgccc
    41521 attttccttt aacattttca tcggcagtca ccatgctgtt gtaggtgcct gcaaacaatc
    41581 ggtatattat aaatgcaaca acaagtaaaa taccaatgat tactactgaa cgtttcatac
    41641 tatatgattt atggtttata agttaacgtg ccaaaactaa aatgtttgat aattcgcttt
    41701 ttgtctaata ggtaaaagtt ttaaaattgt aggtgaatag tattattttg cggataagct
    41761 aaaaatcttt gaagcaaaca aatttcttaa cattgcaatt cattgtttaa tagctgaatg
    41821 ataaagactg tagcaattga aattacaggt aaagtgcaag gtgtgttttt tagaaaattt
    41881 acacaacaga caggtcgtag attgaacctt tgcggttttg ttcgtaaccg ctctgacggt
    41941 tcggtttatg ttgaagcaac aggcagcgaa ttgcagttaa acgaattggt agcatggtgt
    42001 cataaaggct cacccatgtc tttagtcagg caggttgttg tcagagaaat ttctgtcatc
    42061 cacaatgaac catttcaaat tctgtagtaa gtgaaagtaa attataaaac ggctacttta
    42121 gccgattcat gagtggaata aagaagttag ccggtcaaac ggtactttat ggtctgagta
    42181 gcattgttgg gcgtatgctt aattatttac tcgttcctat ttatacccgc attttttctc
    42241 cggcagaata tggtgtagtt tccgagcttt attcctattc agcatttctt ctgatactgt
    42301 ttacctacgg catggaaaca gcttttttta atttcatgca gaaagaagag aataaaaata
    42361 aggtttatga aaattgtatt tcattagttt cttatacaac aacaattctg gtgttgctga
    42421 tgatattttt ctcatcgcca ttggctgcaa ctattgagta tcc
//
LOCUS       DAOOOL010000006        41203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_64_length_41203_cov_149.537134, whole genome shotgun sequence.
ACCESSION   DAOOOL010000006 DAOOOL010000000
VERSION     DAOOOL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 41203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_41203_cov_149.537134"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1190
                     /locus_tag="PKU82_01055"
     CDS             75..1190
                     /locus_tag="PKU82_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81489.1"
                     /translation="MDKRFTVRRGRLKVQYDGKPISKGWSITQYVLQIDATEKGLALK
                     DAYVKLTDPWVGWLSLTTGMQNRPFGYEIPFSSSTRESPERGRMSQIIFPGERDLGMM
                     FTIQAPKSSQWHFIKVDAGMFNGTGVRNAGLDASDFDNIKDFIGRASISKSTHSENIK
                     YGLGMSYYYGGYRIDTVGVYKTGVDVNGVEGFVQQYKKSDFYNVSLSNRKQAYRKYYG
                     FDAQFSIDWFPGITTIRAEYIAGDQPGFSATTVSPASVNTSDIYKRKFNGAYFYFLQS
                     IGHTPLQAVVKYDWYDPNTEVKGNNIGRNVVTGKAFNATDIRYDTWGFGLAYRWDDNI
                     KITAYYDIVKNEKSKNLSGYQKDLKDNLFTLRIQAKF"
     gene            1213..2079
                     /locus_tag="PKU82_01060"
     CDS             1213..2079
                     /locus_tag="PKU82_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOZ81490.1"
                     /translation="MKQLKFFAAGIFLITACNSAKQNNNSEVTNDSKKTAITIKGSDT
                     VLPLSQKEAEAYMKEHPETSITIVGGGSGVGISALLEQTTDVAMSSRPLKIDEKLKFK
                     GKNINPVETIVANDALAIVVHLSNPVDKLTHEQIADIYTGKITNWKEVGGKDAKIICY
                     SRETSSGTYEFFKEHVMDKKNYASSVLNMPATGAIIQSVSQTEGAIGYVGLAYETKDV
                     KHVAVSYDQGKNYVAPSIASAQDGSYPISRPLYYYYNQDKKDAVSSFVDFILSEKGQQ
                     LIQDVGYVPLKK"
     gene            2145..3299
                     /gene="pstC"
                     /locus_tag="PKU82_01065"
     CDS             2145..3299
                     /gene="pstC"
                     /locus_tag="PKU82_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOZ81491.1"
                     /translation="MMSGTVTTLAVLFIVFYLFKESLGLFNMTPVEDSYSVLVNKNNP
                     VKKLSAQQIKLVFDQEITNWKQLGGNDAEIELFRTEDISNYYSEDEIGKNFEHLAQRI
                     NEHIEKHRGIIAFVASEYEMKNAASVPLSVDKITLLELMKGKEWFPTAQPAAQFGVLP
                     LLMGTLLVSLGAILLALPFGIAAAIYLAEIADTRFRNIAKPLIELLAGIPSVVFGFFG
                     LVVIVPMVQQWFNLPVGETALAGSIVLGIMALPTIITVSEDALHNTPRSMKEASLALG
                     ANKWQTILRVVIPYSISGITTAVILGIGRAIGETMAVLMVTGNAAVIPHSILQPVRTI
                     PATIAAELGEAPFGGLHFKALFFLGCVLFLITMIINFIVELISPKRRIER"
     gene            3301..4167
                     /gene="pstA"
                     /locus_tag="PKU82_01070"
     CDS             3301..4167
                     /gene="pstA"
                     /locus_tag="PKU82_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOZ81492.1"
                     /translation="MNYEFKKRLKQNIAFWLLRIFTFSVLIILFVILGFIVKKGIGVI
                     SWEFLTEMPKDGMTSGGIFPAIVGTLLLVLGSMLVAFPIGIMSGICMSEYVKDGFIKR
                     FVTVMTNNLAGIPSIVFGLFGMEFFVNELKWGDSIIAGSFTLGLLAIPIVIRITEESL
                     KSVSKTFREASMALGATKLQTTYKVVLPIAMPNIVTGLILSLSRVSGETAPILFTVAA
                     YFLPKLPNSIFDQVMALPYHLYVISTSGTNIEASRHMAYGTALVLIIIVLMFNLLANW
                     LRKYLSRKVKMN"
     gene            4251..5033
                     /gene="pstB"
                     /locus_tag="PKU82_01075"
     CDS             4251..5033
                     /gene="pstB"
                     /locus_tag="PKU82_01075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOZ81493.1"
                     /translation="MQEPSKQNRTVKIESRNMELYYGEFHALKGINIAIKTNSVAALI
                     GPSGCGKSTFLRSLNRMNDLIDGVKISGQVLIDGKDIYNSKALRIEELRKKVGMVFQK
                     PNPFPKSIFENVAYGLRVNGISHKSFIEERVERSLKQAALWDEVKDKLKKSAFELSGG
                     QQQRLCIARALAIEPSVLLMDEPASALDPISTAKIEELIYELKSDYTIVIVTHNMQQA
                     GRVSDMTAFFYLGELVEYDSTKKIFTNPEDLRTQNYITGRFG"
     gene            5091..5780
                     /gene="phoU"
                     /locus_tag="PKU82_01080"
     CDS             5091..5780
                     /gene="phoU"
                     /locus_tag="PKU82_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOZ81494.1"
                     /translation="MTHLDVEIARLKKDMLDLFGMVKLQLEKSKQALIHLDKDLAREV
                     MVNEKRVNAVEIKLDRDCENIIALFNPVAIDLRFVLACLKIINNLERTGDIADGIARY
                     VLNIKLEPAQKLIDTTRLVEMYDISCNMLDVVMTAFAEENTNLARSVFGMDDKLDEIF
                     HQSNQKVGEFIKENPDRINQSLYVFSASSKLERVGDQCKNIAEELIFFVEAKVLKHSV
                     KSTSKSENQKT"
     gene            complement(5789..6010)
                     /locus_tag="PKU82_01085"
     CDS             complement(5789..6010)
                     /locus_tag="PKU82_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81495.1"
                     /translation="MYESSTTHDLILYYFNEVELADTVLIQHEIDHNPETEENFQEIV
                     QVMDHIDNCMVNPSASVIKNILNYSHSLK"
     gene            complement(6076..6660)
                     /locus_tag="PKU82_01090"
     CDS             complement(6076..6660)
                     /locus_tag="PKU82_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ81496.1"
                     /translation="MQQPVLNDRELVEMYQQGNEDCLTVLIERHQKRIFSAILFLVNN
                     RELAEDIFQDTFVKVINTLKKGSYNEEGKFLPWVLRISHNLVIDHFRRNQRMPMVHDK
                     EDYSIIESLQLMDDNVQDKIVKEQIYKEVRLLVEELPYEQKEVIILRHFADMSFKEIA
                     DATGVSINTSLGRMRYALINLRKVIEKKKLVLAA"
     gene            complement(6908..7327)
                     /locus_tag="PKU82_01095"
     CDS             complement(6908..7327)
                     /locus_tag="PKU82_01095"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOZ81497.1"
                     /translation="MMSNITFTMLKPDAVSNGYIGAILDHITKAGFKIKAMKYTRLTA
                     ERAGEFYAVHRERPFYNDLVKYMSSGPIVAAILEKDNAVEDFRKTIGATDPAKADAGT
                     IRKLYAKSIEANAVHGSDSNENAAIEGSFFFSMTERF"
     gene            7430..8473
                     /locus_tag="PKU82_01100"
     CDS             7430..8473
                     /locus_tag="PKU82_01100"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOZ81498.1"
                     /translation="MIPKFEQHSIEQLKNLLSEPKRIVITTHYKPDGDAIGSLLGLYN
                     FLVAKKHKVKAVVPSDYPASISFMKGNQKVLDYEAKPRNVAKAFSEAEIIFCLDFNES
                     KRVEAMEEVMMSNKAFKVVIDHHLYPKIECDILFSFPKSCATSELIFHFLYALDNKYK
                     LKPEVAECIYTGIMTDTGSFRFSSMSADTHRVLAALLDTGMNHVKIHETISDNFSEER
                     TRFLGYCLKDKMQVIHAYRAAYIALTKNEIEQYNHQTGDTEGIVNYPLSISDMMMSAL
                     FTEKDDMIKISFRSKGDFSVRELASKFFNGGGHQNAAGGKFFGSLDEAVEHYIAALAY
                     YKDQLQIPDTVKD"
     gene            8514..10178
                     /locus_tag="PKU82_01105"
     CDS             8514..10178
                     /locus_tag="PKU82_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOZ81499.1"
                     /translation="MNQSVQQGHPKGLYVLFVTEMWERFSYYGMRAIFILFMVKALML
                     DKAFASNLYGSYTGLVYLTPLLGGYISDRYWGNRRSIIIGGLLMAIGQFTLFFSGSMI
                     SSTPTGTDATASMLMYLGLGFLIFGNGFFKPNISTMVGQLYPKADKRVDGAFTIFYMG
                     INLGAFFAPLVCGYFGDTNDPADFKYGFLAAGIGMVIGVLTFVMLKNKYIVSPDGTPI
                     GMPKVATAATANQEGEVDIPSFNMSKILMWLAIGIGLFFVFKSLIEFDLIGSIIYAGA
                     IAVPGLIITDKSLTHVEKQRIWVIFILCFFVIFFWSAFEQAGASLTFFADEQTDRNLF
                     GWEMPASFFQSFNAIFIVIFAPIFAWLWVRLGNAGKEPGSPLKMAIGLFLLSIGYLVI
                     AFGVKGVEPGVKVSMFWLTTLYLLHTFGELCLSPIGLSMVAKLAPLRFASLLMGTWFL
                     ANSASNKFAGTLSSLYPPGQGEFKAAADKGIDLPSILNGSVTATADQLKVLAEAGIPH
                     HFPMFMGMQISSLYDFFMIFVIMSAAASVLLFIIYKWLVKMMHGVQ"
     gene            10283..10516
                     /locus_tag="PKU82_01110"
     CDS             10283..10516
                     /locus_tag="PKU82_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81500.1"
                     /translation="MKKLFTLMAVAAMFAMVSCGPSADEKAAAEKATQDSIANAEAEM
                     KAAEEAAMAASAAAATPDSAAAAAPADSAAAAH"
     gene            complement(10546..10725)
                     /locus_tag="PKU82_01115"
     CDS             complement(10546..10725)
                     /locus_tag="PKU82_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOZ81501.1"
                     /translation="MAKLTLIEVALFYINMQEIIVAIIAIAASVYLIYKFRSKSAGCD
                     KCSSGPQKSGQKKTT"
     gene            10743..12719
                     /locus_tag="PKU82_01120"
     CDS             10743..12719
                     /locus_tag="PKU82_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOZ81502.1"
                     /translation="MNFDRKDLSNDQLIKLYHELVRPRIIEEKMLVLLRQGKLAKWFS
                     GIGQEAVSVGAAMALHQDEYVLPMHRNLGIFSVRNIPLNRLFSQWMGKPGGFTNGRDR
                     SFHFGTQEYKIIGMISHLGPQCGIADGIALGNILKQNNKATIVFSGDGGASEGDFHEA
                     VNVAAVWDLPVIFIVENNGYGLSTPSSEQYRCKSFADKGIGYGIQGVSVDGNNILEVY
                     NTLNELLTDIRKNPRPIIFEARTFRMRGHEEASGTKYVPQQLFDEWGKKDPIANYENY
                     LLENKIISSSEVESIRAKIKTEIEENLKISYAESDPEVNTVNELRDMYAPSENTNITA
                     SGAVTEKRFLDAITDGLRQGMQRHKNLVIMGQDIAEYGGVFKATQGFVEEFGKPRVRN
                     TPITESSILGASLGLSINGFKSIVEMQFADFVTCGFNQIVNNLAKTHYRWGQNADVVV
                     RMPTGAAVGAGPFHSQSTEAWFFHVPGLKIVYPAFPGDAKGLLAAAIEDPNPVMFFEH
                     KALYRSVSGLVADDYFTIPIGKANKIREGSDLTIVTYGLGVHWAMEALDENKNIQADL
                     IDLRSLLPWDKETVLESVKRTGKVIVFHEDTLTGGIGGEIAAEISSACFQWLDAPVMR
                     CGSLDTAVPMSNHLEWNFLPRDRFKQMLKQLAEY"
     gene            complement(12728..13351)
                     /locus_tag="PKU82_01125"
     CDS             complement(12728..13351)
                     /locus_tag="PKU82_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81503.1"
                     /translation="MQFRQILIFILCICTIQLQAQTLVNSGFEEDANTSSQKGWITDC
                     KGAGQIKVSNISPFSGKQCLEMAHQNQATCTFSQSIIIENTAIKNYKIIYSCRAEALN
                     GNTGLNIIIRDASGKKLTESSGFIKNAEAKWKTYSVAMAVPDGAQTMSITGILNGTGN
                     VWFDEIKVEELKQHKPSSGIWSTASEFMTIAGKYSIGEAIKWAVEKK"
     gene            13469..15625
                     /locus_tag="PKU82_01130"
     CDS             13469..15625
                     /locus_tag="PKU82_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOZ81504.1"
                     /translation="MFRYSLALFFLISTSTSQAQHKKLSMEDAILNAKTNLAPANLSQ
                     LGWIKNSISYYYVDNRLADATLFVGDDGIVHDEKIISLSEINDRLKRLKTDTLARFPA
                     VEWKNDKVFGFFIGNKEYEYDKTQYAIRNSRILLLPDSASQKDTDPVTKRIAYTIKNN
                     LFVYDAGKNFTVAEDAYPHIVYGSSVHRNEFGITKGTFWSPNGNKLAFYRMDETMVND
                     YPIIDWNKKPAEVNMIKYPMAGGTSHQVTVGVFDMANKTVVYLKTGEPKDQYLTNISW
                     SPDEKYVYVAVLNRDQNHMKLNCYSSSTGHFVKTLFEEKDDKYVEPQTPMAFVPGHDD
                     QFIWQSQRDGYNHLYLYNTDGKLIRQLTKGEFIVTEFKGFDKKSERAYYMCTALSPID
                     RDLYYVTLADGRMKRVSGDSGMHTCYYNPENNEVLDVFSNVNTPRKIKLILPNGSDAR
                     TMLTAENPLSDFNLGGMKISTLQSVSGELLYTRTYYPPVMDSTKKYPVIIYVYGGPHL
                     QLITNSWQGGQGDLWFYYLAQEGFIVYTVDSRGSGNRGKQFEQSIFRNCGNAEMEDQL
                     TGLKYLKSLPYVDSTRIGVDGWSYGGFMTISLMTRFPGLFKVAVAGGPVIDWSMYEIM
                     YTERYMDTPATNPEGYEKSNLLNYVKNINGKLMLIHDTNDETVVWQHSLMYLKKAVDL
                     GKQVDYFVYPGHPHNVRGKDRVHLMNKITDYFKQNL"
     gene            15642..17552
                     /locus_tag="PKU82_01135"
     CDS             15642..17552
                     /locus_tag="PKU82_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ81505.1"
                     /translation="MSKKTIFNLLLCFCVVSIHAQNLKRCPQTTSKEAAKKTEEAEKL
                     LKSVKNYEEGVELCNEAIEADSTFAKPYLLMGDAAYRKKDFKLMAKAYKGLMNVCPDA
                     SEKAIYRLATYYFDTKKYEEAIPVYTTFLELSPSDTNAADDAEVKLFRAKAFLHPVDF
                     KPVLVKGLSTPDPEYLPIISADNELCFFTRRFEYQSKNMLTSTSVEKFMLSHNLNGKW
                     DTGTPMDLPFNKQNTGNEGGPTISLDNLHLFFTVNVKGNFDIYTSDLTPKGWSEPRSI
                     GKANDPKFWDSQPSLSPDNKTLYFASFRDSVLQTSDIYVTKKNNGEWNSANPISGPIN
                     TSRNEKAPFMHPDGKTLYFSSDGHPGMGGYDIYYSKKDANGNWGKPVNLGYPINTDAD
                     EVGFFVSTDGTKGFFASNKFGTGGYDIYSFDMPVEARPEKMVLFKGQLKNDDNEVPDS
                     ARIEIRNISSKQVEQIDYDAYSGKYAGVLQANSEYLLTIQKDDYAFTSKLIDAAKVDS
                     PAVTVELDLRKIKEGEAYSLNDILFETDSYQITDKSKAVIQDFAGFLKSNPKLKVAIY
                     GHTDNSGKAEDNLLLSKNRAKAVYDYLIALGIEATRLSHNGFGQTKPVAANTTEKGKS
                     LNRRTEFVVLKK"
     gene            17557..18051
                     /gene="idi"
                     /locus_tag="PKU82_01140"
     CDS             17557..18051
                     /gene="idi"
                     /locus_tag="PKU82_01140"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOZ81506.1"
                     /translation="MEFVILVDEQDSEVGEAEKMQTHKLGLLHRAFSVLIYNSNGEML
                     LQQRAETKYHSGGLWSNACCGHPRPGENIEDAAKRRLKEELGFNCDLKFSFKFLYKVS
                     FENSLKEHELDHVFTGSYESEVLPDKSEVQAFRWITMNNLKNEITENPDKFSVWFLKI
                     ISEL"
     gene            18193..18795
                     /locus_tag="PKU82_01145"
     CDS             18193..18795
                     /locus_tag="PKU82_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ81507.1"
                     /translation="MPVLAFAQSGQEKLVQLSGVIVTGDSLSPVPYTGIMIKSSSRGT
                     VSDYYGFFSIVAHVGDEIEFSSVGFAKTIFKVPDTLTENRYSLIQILNRDTVLLNQAV
                     IYPWPTREQFKQAFLDLKVPDTDMDHARRNLALETMREESIRIDMDGSMNSKFVYQKQ
                     QSRLYNTGQLPVNNLLNPIAWAKFIQAWKSGEFKSKSKDD"
     gene            18795..21320
                     /locus_tag="PKU82_01150"
     CDS             18795..21320
                     /locus_tag="PKU82_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81508.1"
                     /translation="MRQLIVFLSLLFCCYSLNGQDATARIHGKITGTDRKPLPGVSVG
                     IAGTSLGVISDEHGEYSITVPSNRELSIVFSSIGFEQATVKLILAEGEKKQLNRTMLP
                     SIVPLPNITIIDPRERSGTLIKIDPKAVEEIPNPSGNFETILKTIGSGVVSNNELSSE
                     YSVRGGNYDENLVYVNDVEIYRPQLVRSGLQEGLSFINPDLVSGIKFSAGGFDAFYGD
                     KMSSALDIKYKRPTEFAGKITYGLLGGGLSLEGTSKNNRFTYLIGARQKSTKYLLGTL
                     DTKGNYKPVFYDVQALLTYDVSEKTTISLLGNFSKSRYNIVPQTRTTDFGTVSQALRF
                     TVYFDGKEVDDFTTTTGAVIFSHSPKKNINLKLIGSVFNTKEEETFDVLGQYFLDELE
                     KDPASQNFGEVKLNLGVGSFLNHARNFLDGKVYSAEHKGTYNYRHNELLWGIRFQHEQ
                     FSDQIKEWNYIDSSGYSLPHPQDSVGGSLPSQPQLLLQDVISRDNSLSSNRLSGYLQH
                     NFYFSNTSNFILTPGIRFTWWSLNHVLNISPRIALAYKPRWKRDFNFRLSGGFYYQPP
                     FYKELHNPDGSLNTSLKSQQSIHVVGGTDYYFKAWGRDFKFVTEAYYKWLNDLIPYKV
                     DNVRIRYLGKNNSKGYATGIDFRINGQFVNEAESWASLSLLKTEEDIQDDVYYIRLNA
                     RGDTIRGYTIDKKAVDSIQVIPGYLPRLTDQRLTFSIFFSDYLPKFPTFKMHMTLVFG
                     TGFPVGPPGNNRYTDINRYPFYRRVDIGFSKIFIDENRPAKSRFAAFNKLKALSLSIE
                     VFNLFQVNNVVSYTWVSDVYGTKYGVPNYLTGRQLSLRLSAKF"
     gene            21463..22182
                     /locus_tag="PKU82_01155"
     CDS             21463..22182
                     /locus_tag="PKU82_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOZ81509.1"
                     /translation="MRNHSESRFLTERQSRIGEFLFTLKVVREFIKGFRTLHFVGPCV
                     AVFGSARFKEEHHYYQLGREVGANVSRLGFTVMTGGGPGVMEAANRGAKEAGGFSVGC
                     NIILPREQQPNKYLDKMVEFHHFFVRKVLMFKYSYAFVVLPGGVGTIDEMFEALTLIQ
                     TKKVSDFPVVLMGKNYWQPVIDQLKVFDEHKTALHSELDSLLITDSLDEMTEYLRVKA
                     IERFKLSKAMKFNRSKWFLEE"
     gene            22278..23282
                     /locus_tag="PKU82_01160"
     CDS             22278..23282
                     /locus_tag="PKU82_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOZ81510.1"
                     /translation="MKVFRLCFFIFISSMLQAQDIHFSQFYNSPLTLNAANTGLYEGD
                     YRFANTYRSQWSKLDPGYRTNAFSFDKTLYTGADQISGGFLFIYDRSGINRLQSIRLG
                     LSAAYHRTFGVHSLSAGFQWNYIFKSIDRSKITMPEQFNMNTGYFDPSMPTGEMLLDE
                     KRSFGDINAGLVYQVELNKHFIQAGASFFHLNKPGDTFFNSSNKLPVRTCLTVSDKIG
                     IGNDYQLHTRLLMMQQVKANEYVAGVTLVKQLNNGESKFTEVNGGLYVRTSPGNGTDA
                     VIPVIGIKYDLFDVALSYDINISTLHTATASRGGFEISLIYTALSSRLFKIKIPCERY
                     "
     gene            23269..25356
                     /locus_tag="PKU82_01165"
     CDS             23269..25356
                     /locus_tag="PKU82_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ81511.1"
                     /translation="MNAIKTRAILLLILLISSVHLYAQQNDELKKYGTAQLKRMGSYA
                     AKINDFSSSAMYYEEYCKRKPTNEKVWFLYAESLRKSKDYRKAEDAYMKAYNLNPKLN
                     ALALYHYANVQKSLGNYKKAEEYFTKFKKEYKGSDKSVYTKLIKNSGDAARFADSLMH
                     SPSKVAIEHLPEDINSSHVESSPFYINDSTIIYGSLKTDQVTFDFNPRDSSADEPFRK
                     IYVAVRDSEIWKDDGELPGFYNSNSAHTLNGVYSQDKSRFYFTRCKRNRQNKVICAIY
                     VSFLNNGIWTEPVSLGTEVNDPKYTSTQPAIGLNTQKKGEEIIYFVSDREGGKGGYDI
                     WYTSFDNRSKTYKTPVNLGTKINTSGDEVTPYVDAQTQTFYFSSDGWQGLGGLDIFKS
                     IGGMKKWTHPVNLGYPVNSNYDDLYYVLSPSNKDIGMIVSNRNVTDNGMNKGPCCDDI
                     FEFNILDAVRLNVKGFLYETDSTQKGKKLAMMPVRRARITLEMKNAEDSTFIPVSTSN
                     TDETGHFNLIVSPRQEYRITARKEGYLYESVALSTMNKTRSGDISIDIHIRSIPETPL
                     LLTNIYYEFASPLLTAKAMAALDTTLVIFMQRNVDLGVEIRSHTDAIGSDDANMTLSQ
                     KRAESVVNYLVAKGINANRLRAKGFGETMPVAPNTKPDGRDNPKGRQLNRRTEFKILG
                     KMRDGVIREETDF"
     gene            complement(25359..25943)
                     /locus_tag="PKU82_01170"
     CDS             complement(25359..25943)
                     /locus_tag="PKU82_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HOZ81512.1"
                     /translation="MIYEFNGFRPVVDSSSFVHEQATVTGDVIIGKQVYIAPGAAIRG
                     DWGTIIIEDGCNVQENCTIHMFPGVTVILKQGAHIGHGAIVHGATIGKNSLIGMNAVI
                     MDNAVIGDGCIVGALCFVPDGMIVPDRKIVVGNPAKIIKDVSDEMLHWKTKGTELYQQ
                     LPHELTATLKPCAPLRSIEPNRKKHDAVYTTWKK"
     gene            26078..30511
                     /locus_tag="PKU82_01175"
     CDS             26078..30511
                     /locus_tag="PKU82_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81513.1"
                     /translation="MSKYFASIILVFIFLTSTLRAQTLKSFNTDPSSYLLDLKSFFEE
                     TNKKEAEKIMEEFKPVFLGKFNAQQQQTIITTSNLMLKKRMKAFPDFVTYTSVLNAFA
                     SSGQEAATFTSWNSSFEKAIAKLSVRKLGDFLEISQLLFRNNTLYESSAVAWSASNNK
                     FSFGFDSLPKVTFSGMTLRCLGKGDSSVIEGTKGVYYPNNLLFFGDGGTVNFTRAGIS
                     VSEANAVVKRYAINLKGSEYSMDSVAFTYKKYFDQELKGRYIDKLLANVNDSNATYPR
                     FYSYAANLDIKNMVKDADYKGGFSLQGSKMVGSGNRRQDASITFNLNGKPQLKLLSQG
                     FIFRTDRIVSVNAAAVIYWEKDSIYHPGVEFKYIYGDKTVTLTKNGQTAINSPYFDSY
                     HKMDLDFDQLVWKITDPLMDLKMISGGGESKLKFESVNFFSRQRFDKIQGLSEVHPLF
                     KIKQYAEEHNVKVISVPEFAEYLKLTENTVRNQILQLSSLGFISYDADADKFIVKDKV
                     MYYLNARTGKVDYDELKIESMISGLSNATINLLNFDINMRGVSHVLISDSQEVYIQPL
                     DQEMTLKKNRDIVFNGRVHAGRLDFYGKEFTFNYADFNIDLKNVDSLRFKVPADSVDE
                     RGKKRLIPIKSILQQLNGKLSIDNPTNKSGFVPNEEYPVFVCTNKSYIYYDYPEIFGG
                     VYKRDKFYYHLDPFSIDSLNTLTKEGLVFEGSMVSADIFPEWRQQLTVQRDYSLGFIT
                     NTPDAGFAAYSGKGKYRDQISLSHEGFLGKGEVDYLSSTSKSNNIIFFPDSMHAVTQS
                     FAIRKEQVGATRFPDVKNEDVFVNWRPKSDKMFVFKRSKDFALFNGEAVLDGNLVLMP
                     SGIKANGIIRYQQSQLISNNHFIKEHSYGSDSAEFKLNTDAGDLLALHTQNVKANIDL
                     DKRIGDFSSQGEKAKVEFPVNEYISYIRDFKWYIDKSELEFGGSDATADKANEFVSVN
                     RQQDSLRWDAPLATYNLKSYLLKGFKVKEILVADASIIPDLGNVVVERNAVMRPLVNS
                     KVIANTTSHYHTMLNTNITIQSRMNYSGDGNYEYMDQTKVKHKIKLTQIGVDTSKQTF
                     AIGEIPDSSRFFISPTIQYKGKLRINAPKENLYFNGFAIVNHHCNTFERNWFGFAGDI
                     DPKGVNIPIQSPANESLQKLYSGIYFGSDSTMMYPAFLSMKKHSSDAEVISAEGYLTF
                     DNNLQQYKISGTIPGTDSTKSASVITNTVILDDKRCQLIGEGRINLTQDFGQLSITNF
                     GMVNYSTVNDSIGFDAVMATDFMFNDDALKQMAEMFNSIPALQPFMDDRPAYRNALTN
                     MIGKNKAEKLLNETGLYGSARRIPSELQHTLMLSELKFYYNKERASYISVGNIGLGVI
                     GKTYVGRMVKGKVEIMRRRSGDVLNIYLEALPDMWYYFNYQRGVLQAISSDEKFNKPI
                     DEMKPEKRIASEKGGLEPYQFMLATERKKNEFLKRIKDADL"
     gene            30567..31040
                     /locus_tag="PKU82_01180"
     CDS             30567..31040
                     /locus_tag="PKU82_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ81514.1"
                     /translation="MQREHHYQANIIWTGNKGSGTDSYLGYARSYTVNIDNKPEIQGS
                     ADAAFHGDKTKYNPEEMLLIALASCHMLWYLHLCSEAGVVVVEYTDRATGLMTETPSG
                     NGKFESVTLNPIVVIRDQTKNEIAIELHKKANEFCFIANSVNFKVNHKPVFKLMK"
     gene            31192..32160
                     /locus_tag="PKU82_01185"
     CDS             31192..32160
                     /locus_tag="PKU82_01185"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOZ81515.1"
                     /translation="MKNTFFTVAFILCAFFVKAQSSNVILFTENGEKFFAILNGVRQN
                     TSPETNVKVTGLNANFYKLKVIFEDTKLGEKDFNLAFNPGMETTFSIKFIAKKNSYVL
                     RPLSEVPLAQALPPAANQTVVAYSEVPAPPPAATTVTHTTTTTTTDGTPMPGDNVNVN
                     IGVNMNEAGGGINMNVSGGTTQTTTHTTTTTVTHTTTSEPTAPPIAPPPPAPVVYLPG
                     YNGPYGCPVPMAPQDFSELKQSISSKSFEDSKMTIAKQVLRNNCLFTSQVKDLMGLFT
                     FEESKLDFAKYAYDYTYDIGNYFKVNDMFTFESSIDDLNNYINSKH"
     gene            complement(32226..32672)
                     /locus_tag="PKU82_01190"
     CDS             complement(32226..32672)
                     /locus_tag="PKU82_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ81516.1"
                     /translation="MVIREAKIDDIKQIQRVRNSVKENTLSNPDLVTDKDCAEYISER
                     GKGWVCEIENQIVGFAIADLKENNIWALFLDPKFEKMGIGRHLHKTMLDWYFSQTKQT
                     VWLGTAFNTRAEQFYRKAGWTEVGTHRSKEIKFEMTYSDWIKIPNH"
     gene            complement(32757..34592)
                     /locus_tag="PKU82_01195"
     CDS             complement(32757..34592)
                     /locus_tag="PKU82_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOZ81517.1"
                     /translation="MRLIRNFLVLSIFWMLFFGIFRIIFFLFLQYNAANIQLNELPST
                     LFYGIRLDISATAYISALPLLLMLIHALTENTFIKKLFLVYNGIILFVLILLHVSNLF
                     LYRSWGVLINYRALSFLADWRQMFLSVNNFELIVGIICIILLFILTWFVFRKFTKHFF
                     DNKLAIIQSLLQFIVSAAMLVIMFRGGVQMLPVNESAAAFSENVTLNHIATNPAWHLG
                     HNLNQAKSENASYYKFYSDAVAEEHVKKLFQTPIDLMPVLNNKKPNIVIIMLESFTVD
                     VVGSMGGETGVSPTIDSLIHDGLSFKNIYSSGARTDQAFVAVLSGFPAQPNQSIMRFP
                     DKTAKLPSLPAELKKQGYSTSFYYGGDLDFSNLNTYLVNCGFEQITGKKKFKQQQLSA
                     KWGAHDEFVFEKNLNDLNQAKEPFFSVVMTLSSHEPYDIPIQSPFGKDNPNEQFKGSA
                     WYADHSLGNYLAAAKKEPWFKNTLFVIVADHGNKIPRDRSYYSHYIRHIPMVMAGDVV
                     KNIYKNKIVYSTGSHHDIPSTLLHQLGFNSDMFNWSNDLLNRKRNGFAYLCMDDAEGW
                     VTDSCTFVYDLSNQKFIYKRPYYAIADTVTAKSYLQQLYSTFSNL"
     gene            complement(34594..35334)
                     /locus_tag="PKU82_01200"
     CDS             complement(34594..35334)
                     /locus_tag="PKU82_01200"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOZ81518.1"
                     /translation="MAKIINWFCFLLLLVFCSCQKDHMFDCFKGTGNDKSEIRNITGF
                     NRIKASNNVDVKIYPGYDYQVEVMAGSKLIEGITTEVKDSMLYIRNENACNWVRSFKN
                     KFTVKVWMPDIAELTVNGSGNIDLMDTIRHNEFTFNNWGASGKVTFLFNTGAVRTNIH
                     TGPGDFVFHGYVGVHYFYNNGNGIADASNLNTEVTFTENKGANKEYVRAKQWLTAKIS
                     YVGDIYYFGNPDSVVHEGTGNGKLIKAD"
     gene            complement(35318..36271)
                     /locus_tag="PKU82_01205"
     CDS             complement(35318..36271)
                     /locus_tag="PKU82_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOZ81519.1"
                     /translation="MSELTIPVDLENPVAFFGINDSNLDLLRKKFPAFKLIVRGNELK
                     ILGDEKQIHWVAKKIIGAIEYFHHYGKFDEQVLEKLMADGQNSDTLVNPPSGNGSDVL
                     VFGPNGVVVKARTANQQKMVDSCNKNDIVFAIGPAGTGKTYTAVALAVRALKNKEIKR
                     IVLTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALFDMIPGEKLKTFIENGVIEVAP
                     LAFMRGRTLDHAFVILDEAQNTTESQLKMFLTRMGPAAKFIVTGDVTQIDLPNKQPSG
                     LIQAMKILKNIKGIDFITLDAGDVVRHRLVKDIILAYGKNH"
     gene            36445..37230
                     /locus_tag="PKU82_01210"
     CDS             36445..37230
                     /locus_tag="PKU82_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOZ81520.1"
                     /translation="MSLITLTTDWGLTDHYVAALKGELLSMLPGVNIVDISHQIPAYD
                     FTKAAFVFKNAFYFFPPQTIHLICVDKQSKKTEDTHSGWMVIQYGESIIVCRNNGFFT
                     LVSKQEPLKSVYIEGSEEVSVAHEKSFLVTLLHDLVHHKPLEEIGQPIKSFYQSSMFQ
                     PSHDANTIQGTVIHIDTYGNAITNIEKEYFENVVGAKRFEIDMPRHGDAITKIYNRYD
                     DVTPGQLVALFNASGYLEIALNQGDISLLYGLEYGDKIRIFLQ"
     gene            37227..37535
                     /locus_tag="PKU82_01215"
     CDS             37227..37535
                     /locus_tag="PKU82_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOZ81521.1"
                     /translation="MITRIVRLPVKPDEGKNFEQLFDTIKEKIAAFDGCLSVELHKDV
                     ASSDYFTYSLWQSESHLQAYLDSSFFTDVWPNAKKLLHQRANAWSLEKVLNVWKVAKF
                     "
     gene            complement(37883..39091)
                     /locus_tag="PKU82_01220"
     CDS             complement(37883..39091)
                     /locus_tag="PKU82_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ81522.1"
                     /translation="MTKKYPFHYEVRVKLLAFDKKNNVRNESFIKKFDDKTPLENRAN
                     AFEEFNEYLSFLEQIGRLKKSNQGNFVITQPSFINEILDKKENSEDYFEFKKVYNQYK
                     EEISVYLVVNDLDIAKNVIDAFEFEGKVETEFEIHRVSSSMYDLEPQNLIDSLEMQEV
                     PLYEYYQINVGELKTTVYHYGLDYAESGEDEEGGAKRTILKTPYVWDTIERYNEIYDS
                     NQDTEETDINEGIDLLSIIKRGESNQVEFKPCLLYNFKTQAAGIGVKYLIAKAICGFL
                     NSNGGVLFIGVNDNGNIQGLENYDYSLFHGENEKDKLLLEFDSLLAYFFDLSIKPLVD
                     LSIKKVEGKDILVIVITESYKPIFLKNKKNEFIEKELYIRMNASTRQIVNIEEMVEYV
                     FNKQWKKPSR"
     gene            complement(39093..39641)
                     /locus_tag="PKU82_01225"
     CDS             complement(39093..39641)
                     /locus_tag="PKU82_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81523.1"
                     /translation="MQESTLIPFLRKNLDILFVGLNPAKGSSENGHYLSVNQAFGNQL
                     YESILLTKLVDKSNADELIFGANKYKYQNWNFGITDLITEIAESNSAKIKPKKADLIR
                     LKNVINNYKPKTTVLLHGKVLKKFISHLGYTVPESNTGKLGRLIENCNTTFYNIAFPH
                     GNSITSESKIEKYTELKEFLNL"
     gene            39759..39896
                     /locus_tag="PKU82_01230"
     CDS             39759..39896
                     /locus_tag="PKU82_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81524.1"
                     /translation="MPTIPGIDRHQIIFTDLERQISKDNVIRFIDAFVDKMDLKQLDI
                     L"
     gene            40027..40164
                     /locus_tag="PKU82_01235"
     CDS             40027..40164
                     /locus_tag="PKU82_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81525.1"
                     /translation="MAFRVEYRIVFYRKSLLSQPDGSGLNEVVDFEAQNFQFTTNVDV
                     R"
     gene            complement(40224..40658)
                     /locus_tag="PKU82_01240"
     CDS             complement(40224..40658)
                     /locus_tag="PKU82_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ81526.1"
                     /translation="MDSLFEIHRHLFQDIYAWAGKKRIVEISKDGKQFFPTSHFDNAF
                     RYIDLLIADFKKIPKDNKKMLSEKLTEILDNVNYLHPFRDGNGRTQREFLRLLALEKD
                     LTLNLNPPDNLSVYERYMKGTIDSDVKTLTKLIFELIDTKDK"
     gene            complement(40683..40832)
                     /locus_tag="PKU82_01245"
     CDS             complement(40683..40832)
                     /locus_tag="PKU82_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81527.1"
                     /translation="MSNSYKYIDPDFTYTDPKTGLLRNLQDITDADVLLFVESGAVTK
                     RLQEL"
     gene            complement(40825..41046)
                     /locus_tag="PKU82_01250"
     CDS             complement(40825..41046)
                     /locus_tag="PKU82_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HOZ81528.1"
                     /translation="MFNTIEIDRNNLTIMGVKFSDLKTLESTANALGNSMFEGFKPTP
                     KGIEIIRDYVTGKISLTELVAFAKQKAYV"
BASE COUNT        13143 a         6904 c         7892 g        13264 t
ORIGIN      
        1 gtttgtaaat attcaaaccg ccttcaagtc caaatggaag agaaaaaaga aatggtggaa
       61 actttcctgc cggagtggat aaacgtttta ctgtgcgaag aggacgttta aaagtgcagt
      121 atgatggtaa accgatttca aaaggttgga gtattactca gtatgttctt caaatagatg
      181 cgacagaaaa gggtttagct cttaaagatg cttatgttaa acttacagat ccttgggtgg
      241 gatggttaag tcttacaaca ggaatgcaaa acagaccatt tggctatgaa attccctttt
      301 catcaagtac acgtgaatca cctgaacgcg gacgtatgtc gcagataatc tttcccggtg
      361 aacgcgacct tggaatgatg tttaccatac aggcaccaaa atcttctcag tggcatttta
      421 ttaaagttga tgcaggaatg tttaatggca caggagtgcg aaatgccggt ttggatgctt
      481 ccgattttga taatataaaa gattttattg gtcgtgcatc aatttctaaa tctacacata
      541 gtgaaaatat taagtatggt ttaggtatgt catattacta tggtggatac agaattgata
      601 cagttggtgt atataagaca ggtgttgatg tgaatggagt tgaaggcttt gttcagcagt
      661 ataagaagag cgatttttat aatgtttctc ttagcaatcg taagcaggca tatcgaaagt
      721 attatggctt tgatgctcaa tttagtattg actggtttcc tgggatcaca acaattcgtg
      781 ctgaatatat tgcaggtgat caaccgggat tttccgctac aactgtaagt ccggcttctg
      841 ttaacacttc tgatatctat aaacgaaaat ttaatggagc atacttttac ttcttacaaa
      901 gtattggaca tacaccactt caggcagttg taaaatacga ttggtacgac ccaaatacag
      961 aggtaaaagg caataacatt ggtagaaatg ttgtgacagg aaaagctttc aatgcaactg
     1021 atatcaggta tgatacctgg ggttttggtt tagcatatcg ttgggatgac aatatcaaga
     1081 taactgcata ttatgacatt gtgaaaaatg aaaaatcaaa aaacctaagt ggttatcaaa
     1141 aagatttgaa agacaattta ttcacgctgc gtattcaagc caagttctaa taataaacaa
     1201 ttaaaataaa aaatgaaaca actgaaattt tttgcagcag gtatttttct gattacagct
     1261 tgcaacagtg ctaaacaaaa caacaattct gaagtaacaa atgactcaaa aaaaacggca
     1321 attacaatca aaggaagtga tacggttttg ccattgtcgc agaaagaagc agaagcttat
     1381 atgaaggaac atcctgaaac ttctataaca atagtaggtg gtggttccgg tgttggcata
     1441 tccgcactgt tagaacaaac aacagatgtg gctatgtcgt cacgcccttt aaaaattgat
     1501 gagaaactta aattcaaagg aaagaatata aatccagttg aaacaatagt tgcaaatgat
     1561 gcattagcta ttgtagttca tctaagtaat ccggtagata aacttacaca tgagcaaata
     1621 gccgacattt atacaggaaa gattaccaac tggaaggaag taggaggaaa ggatgctaaa
     1681 ataatttgtt attctcgtga aacgagttca ggtacttatg agttttttaa agagcatgta
     1741 atggataaaa aaaattatgc cagcagtgtt cttaacatgc ctgcaacagg agctataata
     1801 cagtctgtaa gtcaaaccga aggtgcaatt ggttatgttg gtcttgcata cgagacaaag
     1861 gatgttaagc atgtggcagt atcttatgat cagggaaaaa attatgtagc accatcaatt
     1921 gccagcgcac aagatggtag ctatccgatt tcaagaccat tgtattatta ttataatcag
     1981 gacaaaaaag atgcagtaag cagctttgtt gactttattt tatctgagaa aggtcagcaa
     2041 ttgattcagg atgttggtta tgttcctttg aaaaaataac tacagctaag tttgtgaaaa
     2101 caatatttaa aaaagttata tcaaagtttg ttgaaggctt gctgatgatg agcggtacag
     2161 taactaccct tgctgtgctt tttatcgttt tttatttgtt caaagaatcg ttaggtttat
     2221 ttaacatgac acctgttgaa gacagttatt ctgttttggt aaataaaaat aatcctgtta
     2281 agaaattatc agcgcaacaa ataaaattgg ttttcgatca ggaaattacc aattggaagc
     2341 aattaggtgg taatgatgca gaaatagaat tgtttcgtac ggaagatatc agtaattatt
     2401 attcggaaga tgaaataggt aaaaactttg aacatcttgc ccaaagaata aatgagcaca
     2461 ttgaaaaaca tcggggaata attgcatttg ttgcatcgga atatgaaatg aaaaatgcag
     2521 cttctgtgcc actgtcagta gataaaatca cactgcttga attgatgaaa ggtaaggagt
     2581 ggtttcctac agcacaaccg gcagcgcagt ttggagtact tccgctttta atgggcacac
     2641 ttttagtaag ccttggagct attcttttag cattgccttt tggtattgca gccgcaattt
     2701 atcttgcaga aattgctgac acgcgattca gaaatattgc aaaacctttg attgaactct
     2761 tagcagggat accttctgtt gtatttggtt tttttggttt agtagttatt gtcccaatgg
     2821 tgcaacaatg gtttaatctt cctgtgggcg aaacagcatt ggcaggaagt attgttttag
     2881 gaattatggc actgcctaca attattacag tatctgaaga tgcattacac aatactcctc
     2941 gtagtatgaa agaagcaagt ttggctttag gtgctaataa gtggcaaaca attctcagag
     3001 tggtaattcc ttattcaatt tcaggaataa caacagcagt catattaggt attggccgtg
     3061 ctattggaga aacaatggca gtgcttatgg ttaccggcaa tgctgccgta attccacatt
     3121 ctattttaca gcctgtgcgt acaattcctg caactattgc tgctgaattg ggcgaagcac
     3181 cttttggagg attacatttt aaagcattgt tctttttagg atgtgtgctc tttctgatta
     3241 caatgattat aaattttatt gttgaattaa tttcaccaaa acgcagaatc gaacgataaa
     3301 atgaactacg aatttaaaaa acgacttaaa cagaacatag ctttttggct gttgcgtata
     3361 ttcacctttt ctgttttaat tatcctgttt gtaatacttg ggtttattgt taaaaaaggg
     3421 ataggtgtaa tcagctggga gtttcttaca gagatgccaa aagatggaat gacaagtggt
     3481 ggaatttttc ctgcaatagt tggtacacta ttacttgttt taggcagtat gcttgtggca
     3541 tttccgattg gtatcatgtc aggcatttgc atgagcgaat atgttaaaga tggttttata
     3601 aaacgttttg ttaccgtaat gacaaataac cttgccggca ttccttcaat tgtgtttgga
     3661 ttgtttggta tggaattttt tgttaatgag ctaaagtggg gcgattcgat tattgccgga
     3721 tcgtttacat taggacttct tgcaattcct attgtcataa gaataacaga agagtctttg
     3781 aaatctgtat caaaaacttt tcgtgaggcc agtatggcat taggcgcaac aaagcttcag
     3841 actacttata aggtggtgtt gccaattgct atgcccaaca ttgttacggg attgatttta
     3901 agtttgagca gggtgtcagg agaaactgcg ccaatactgt ttacggtggc agcttatttt
     3961 ctgcccaaac ttccaaacag tattttcgat caggtaatgg cattgcccta tcacctctat
     4021 gttatttcaa caagcggaac aaatattgaa gcatcaaggc acatggccta tggcactgca
     4081 ttggtattaa taattattgt gctcatgttt aatctgcttg caaactggct aagaaagtat
     4141 cttagtcgta aggttaaaat gaattaatag tgtatcaaac aaaataaaga aaaatgattg
     4201 taatggaaaa gcaatttaag gataaagagt caatagaaga tgtaatgcaa atgcaggagc
     4261 cttcaaagca aaatcggacg gtaaagattg aaagtagaaa tatggaactc tactacggag
     4321 aattccatgc cctaaaaggt atcaatattg caattaagac caactctgtt gctgcactca
     4381 tcgggccctc cggatgtggg aaatctactt ttctgcgctc gctaaacaga atgaatgacc
     4441 ttattgatgg cgttaaaatc agtggtcagg tattgattga cgggaaagat atttacaata
     4501 gtaaagcgct tagaattgaa gaactgcgta agaaagttgg gatggtattt cagaaaccca
     4561 atccatttcc taaatcaatt tttgaaaatg tagcgtatgg tttacgtgtt aatggtatct
     4621 cgcataagtc ttttattgaa gagcgtgttg aacgctcttt aaagcaagct gcactgtggg
     4681 atgaggtaaa agataaactt aaaaaatctg catttgaatt atcgggcgga cagcaacagc
     4741 gtttatgtat tgcacgggcc ttggctattg agccttctgt tttattaatg gacgaacctg
     4801 cttctgcact cgatcctatt tcaacagcaa aaattgaaga gcttatttat gaattaaagt
     4861 cagattatac tattgttatt gtaacacata acatgcagca agcaggtcgt gttagtgata
     4921 tgactgcatt cttttatctg ggtgaattgg tagaatatga ttctacaaag aaaattttta
     4981 ccaatcctga agacctgaga actcaaaatt atattacagg gcgatttgga taattaattc
     5041 tgttccttat tcagtaaaat tttaaataaa agtaaaaaac aaaacttagt atgacacatt
     5101 tagatgttga aatagcaaga cttaagaaag atatgctcga tctgtttggt atggtgaagc
     5161 tacaattgga aaaatctaaa caggcattaa ttcatttaga taaagatttg gcacgagaag
     5221 taatggtaaa tgaaaaacgt gtcaatgcag ttgaaataaa acttgatcgc gattgtgaga
     5281 atattatagc gctattcaat cctgttgcaa tagatcttcg atttgtcttg gcttgtctga
     5341 aaataatcaa caaccttgaa cgaacaggtg atattgcaga tggtatagcc cgttatgtat
     5401 tgaatattaa acttgagcct gcacaaaaat taattgatac aacaagattg gtagagatgt
     5461 atgatatatc ctgcaatatg cttgatgttg tgatgacggc attcgcagaa gaaaatacaa
     5521 acttagccag aagtgttttt ggaatggatg ataaacttga tgagattttt caccagtcaa
     5581 atcaaaaagt aggtgagttt atcaaagaaa atcctgacag aataaatcaa agtctctatg
     5641 ttttttctgc cagcagcaag ttggagcgcg ttggcgatca atgcaaaaat attgcagaag
     5701 agctaatctt ttttgttgaa gcaaaagtgc tgaagcactc tgtgaaatca acatcaaaat
     5761 cagaaaatca gaaaacttga tattagaatt acttcaatga atgtgagtag ttcagaatgt
     5821 ttttgataac agatgcagag ggattaacca tgcagttgtc aatatgatcc attacctgaa
     5881 caatttcctg aaagttttct tctgtttcag gattgtgatc aatttcatgt tgaatcaaca
     5941 cagtgtcagc taattcaact tcgttaaagt agtaaagaat taaatcgtgt gtagtagaac
     6001 tttcgtacat aggcgtgaaa cttatttaca cctataacgc aacgcaatcc ggaatatttt
     6061 atgattgcct gaaaattatg ctgccagcac caattttttc ttctcaatca cttttctaag
     6121 gttgataagt gcatagcgca tccttcctaa agaagtgttg atactcacac ctgtagcatc
     6181 ggcaatttct ttaaaactca tgtctgcaaa gtgtctcaga atgatgactt ctttttgctc
     6241 gtaaggtaat tcttcaacca acagacgcac ttccttgtaa atctgttctt tgacaatttt
     6301 atcctgaacg ttatcgtcca tcagttgcaa tgattcaatg attgaataat cctccttgtc
     6361 atgaaccatt ggcatacgct gattgcgtct gaaatgatca ataactaagt tgtggctgat
     6421 gcgaagaacc caaggtaaaa atttaccttc ttcgttatag cttccttttt ttaaggtgtt
     6481 gattacctta acaaaggtgt cctggaaaat atcttcggct aattctctat tattgacaag
     6541 aaagagaatg gcagaaaata ttcttttctg gtgtcgttca atcagaacgg ttaagcagtc
     6601 ttcgttacct tgttggtaca tttcgaccag ttctcggtcg tttaaaacag gctgttgcat
     6661 agttaactct tttttggaaa taagtgttga agtagagttt tatgctatag ttttcctcct
     6721 gtttgaaatg ttattgtgag atgttttgag atatagaacg tgttattttt aataatgttg
     6781 tatagctcag aaaatatttt tattaattat tcagtaaaca taaagaaaaa atcctgccaa
     6841 ttcaactttg aggtaataag tgacaggatt tttcagtaaa acttcacatc cgagggtata
     6901 tcggagctta aaatctttca gtcatgctaa agaaaaatga gccttcaata gcagcatttt
     6961 cattactgtc tgaaccatgc actgcattgg cttcaatact tttagcataa agtttacgga
     7021 ttgtgccggc atcagccttt gcagggtctg ttgcaccgat agtttttctg aaatcttcaa
     7081 cggcattgtc tttttcaaga attgccgcta caataggtcc ggaagacata tattttacta
     7141 agtcattata aaatggtctt tcacggtgaa cagcatagaa ttcacctgca cgttctgcag
     7201 ttaaacgtgt gtacttcatt gctttaatct taaatccggc tttggtaata tgatctaaaa
     7261 tagcgccaat gtatccattg cttaccgcat caggcttcag catagtaaaa gtgatgttac
     7321 tcataattaa atgtttgtat ttttttgtgg ctgcaaaagt agttgatttc agtttaggtt
     7381 tgactgacgc aaataaccta catttgtaaa aatcgcattt aaaaagttta tgataccaaa
     7441 atttgagcaa cacagcatag agcaattaaa gaatctttta tcggaaccaa aaagaattgt
     7501 catcaccaca cactataaac ccgatggtga tgctataggc tcacttcttg gattgtataa
     7561 ttttttagtt gctaaaaaac ataaagtcaa agctgttgtg ccaagtgatt atcctgcatc
     7621 aatcagtttt atgaagggca atcagaaagt gttggattat gaagccaagc cacgaaatgt
     7681 tgctaaagca ttttccgaag ctgaaattat tttttgtctt gactttaatg aatcaaaaag
     7741 agtggaggca atggaagaag taatgatgtc aaacaaagct tttaaagtag ttattgacca
     7801 tcatttatat ccaaaaattg agtgcgacat tttgttttcg tttcctaaat cttgtgccac
     7861 tagcgaattg atttttcatt ttttatatgc attagacaac aagtataaat tgaaaccaga
     7921 ggtggcagag tgtatctata ccggtatcat gacagataca ggttctttca gattttcttc
     7981 gatgagtgct gatacgcatc gtgttttggc ggccttactc gatacaggca tgaatcatgt
     8041 taaaatacac gaaaccattt cagacaactt ctcagaagaa cgaacacgtt ttctcggtta
     8101 ctgccttaaa gataaaatgc aggtaataca tgcctacagg gctgcttata ttgcgctgac
     8161 taaaaacgaa atagaacagt ataatcatca aaccggagat acagaaggca ttgtaaatta
     8221 tccactaagt atcagcgata tgatgatgtc ggcacttttt actgagaaag acgatatgat
     8281 aaaaatatct ttccgctcaa aaggtgattt ctcagttcgt gaacttgcct ctaaattttt
     8341 taatggcggt ggacatcaga atgcagcagg aggaaagttt tttggaagtt tagacgaagc
     8401 agttgaacac tatatagctg cattagccta ttataaagac caattacaaa ttccggatac
     8461 cgtaaaagat taattttttt acattagccc cgctttaaat ttaaatcaaa cgtatgaacc
     8521 aaagtgtaca acaaggacat cctaagggac tgtatgtgtt atttgtaacc gagatgtggg
     8581 agcgtttcag ctattatgga atgcgcgcca tctttattct tttcatggta aaagcattaa
     8641 tgcttgacaa agcatttgct tcaaatcttt atggaagtta taccggatta gtgtatctaa
     8701 ctccgttgtt aggtggatat atttcagatc gttattgggg aaacaggcgg tcaattatta
     8761 ttggtggctt attgatggct attggtcagt ttactttgtt ttttagtgga tcaatgatta
     8821 gttctacacc tacaggtacc gatgccacag caagtatgct gatgtattta ggtcttggat
     8881 ttttgatttt cggaaacgga ttttttaaac ccaatatttc tacaatggta gggcagttat
     8941 atcctaaagc cgataaaaga gttgatggag cctttacaat tttctatatg ggtatcaacc
     9001 tgggtgcatt ctttgcgcca ttggtttgcg gatactttgg tgatactaac gatcctgctg
     9061 actttaaata tggatttctt gctgccggca taggaatggt tattggtgta ctcacgtttg
     9121 tgatgctcaa aaataaatat atcgttagtc cagatggtac acctatagga atgccaaagg
     9181 tggctacagc agctacagca aatcaggaag gtgaagttga tattccatca tttaacatgt
     9241 caaaaatatt gatgtggtta gcgattggaa taggattgtt ttttgttttc aaatctctta
     9301 ttgaattcga tttaataggc tcaattattt atgcaggtgc aattgcagtt cccggtctta
     9361 ttattacaga taagtctttg actcatgttg agaaacaacg catctgggta atttttatac
     9421 tttgcttctt cgtaattttc ttctggagtg cattcgaaca ggcaggtgcc tcactgactt
     9481 tctttgctga cgaacaaaca gacaggaatt tatttggatg ggaaatgcct gcaagctttt
     9541 ttcaatcatt caatgcaatc tttattgtga tttttgctcc aatctttgca tggctgtggg
     9601 tgcgattggg taatgcagga aaagaacccg gatcaccatt gaaaatggca ataggacttt
     9661 tcttactttc aatcggctat ttagttattg cttttggtgt aaaaggagtc gagcccggtg
     9721 taaaagtttc tatgttttgg cttacaacat tatacttgct gcatacattt ggtgaacttt
     9781 gtctgtctcc aataggactt tcaatggttg ctaaattagc accgctgcgt tttgcatctt
     9841 tgttaatggg aacatggttc ttagccaact ctgcttcaaa taaatttgcc ggtacactaa
     9901 gttcattata tcctcccgga caaggcgagt ttaaagctgc tgcagacaaa ggcattgatc
     9961 ttccttctat acttaatggc tctgttacag ccactgccga tcagttaaaa gttcttgcag
    10021 aggcaggtat tccgcaccat tttccaatgt ttatgggtat gcagatttca agcctctacg
    10081 acttctttat gatatttgta attatgagtg cagcagcatc agtactgctg tttatcattt
    10141 acaaatggct tgttaaaatg atgcatggtg tgcaataaca attaattgtt aacaacttag
    10201 ttgaatagtt aataaaaaac accttcatca tttattaatc agcatacttt tgtcccgtca
    10261 aatcaaacaa caaaaaagaa aaatgaaaaa attattcaca ttaatggctg ttgcagcaat
    10321 gtttgcaatg gtatcatgcg gtccaagcgc tgacgaaaaa gctgctgcgg aaaaagcaac
    10381 tcaggattca attgctaatg cagaagccga aatgaaggct gctgaagagg ctgcaatggc
    10441 tgcatctgct gccgctgcta ctcctgattc agctgctgcc gctgcacctg cagattcagc
    10501 tgctgctgct cattaatttt aaaaaattaa ggaaaagaac caccgtcagg tggttttttt
    10561 ttgccctgat ttttgcggac cagaactgca tttatcacat ccggcagatt tgctcctgaa
    10621 tttataaatc agataaacac ttgcagcaat ggctattatg gcgacaatta tttcttgcat
    10681 attaatataa aacaaagcta cttcaattaa agttaacttt gccacacttt ttttatttaa
    10741 caatgaattt cgacagaaaa gatttgagta acgaccaact aatcaaactt tatcatgagt
    10801 tggtgcgtcc tcgtataatt gaggaaaaga tgcttgtcct acttcgtcag ggaaaactgg
    10861 caaagtggtt ttccggcatc ggtcaggaag ccgtttctgt aggtgctgcc atggcacttc
    10921 atcaggatga atatgttttg ccaatgcata gaaaccttgg catttttagt gtacgaaata
    10981 ttcctttgaa ccgtttgttt tcgcaatgga tgggcaagcc tggtggattc accaatggtc
    11041 gcgatcgctc ttttcacttc ggtactcagg agtataaaat aattggaatg atatcgcatc
    11101 tcggtccgca atgtggaatt gcagatggaa ttgctctggg aaatattctt aaacaaaata
    11161 ataaggcaac aattgttttt tcaggtgatg gtggtgccag cgaaggcgat tttcacgagg
    11221 cagtgaatgt tgcagctgta tgggatctgc ctgttatttt tatagtagaa aataatggtt
    11281 acggattgtc aacaccatcg tccgaacaat acagatgtaa gagttttgca gataaaggaa
    11341 ttggttatgg cattcaaggt gtaagtgttg atggcaacaa tattctggaa gtttataata
    11401 cactgaatga attattgact gacatcagaa aaaatccacg tccaataatt tttgaagcac
    11461 gcacattcag aatgcgcggt cacgaagagg catcaggtac caaatatgtt cctcagcaat
    11521 tgtttgatga atggggaaag aaagacccga tagcgaatta cgaaaattat cttttagaga
    11581 ataaaataat ttcttcttcg gaagttgaaa gtattcgcgc aaaaatcaaa acagaaattg
    11641 aagaaaatct aaagataagt tacgcagagt ccgatcctga agttaacact gtaaatgagc
    11701 tgcgcgatat gtatgctcct tctgaaaaca caaacattac tgcttcagga gcagttactg
    11761 aaaaaagatt tttagatgcc ataactgacg gacttcgtca aggtatgcag cgtcataaaa
    11821 acctagtcat catgggtcag gatattgcag agtatggcgg agtgtttaaa gcaacacaag
    11881 gatttgttga agagtttggc aaaccgcgtg tccgaaatac acctattaca gaatcatcaa
    11941 tacttggtgc aagtttaggt ttatcaataa acggctttaa atcaattgtt gaaatgcagt
    12001 ttgccgattt tgttacttgc ggtttcaatc agattgtaaa caacctggca aagacacatt
    12061 atcgctgggg gcaaaatgca gatgtagtag tgcgtatgcc cacaggcgct gctgttggtg
    12121 cgggaccatt tcactcgcaa tcaacagaag catggttttt tcatgtgccg ggactaaaaa
    12181 tcgtttatcc tgcatttccg ggtgatgcaa aaggtttatt ggctgctgct attgaagacc
    12241 cgaaccctgt aatgtttttt gaacataaag cactttatag aagtgttagc ggcctcgttg
    12301 ctgatgatta ctttacaatt cctataggta aggcaaataa aatcagagaa ggttctgact
    12361 taacaattgt aacttatggt ttaggtgtac attgggcaat ggaagcattg gatgaaaata
    12421 aaaatattca ggctgattta attgacctga gaagcctttt accttgggat aaagaaaccg
    12481 ttttagaatc tgtaaaacga acaggaaaag ttattgtatt tcacgaagac actttaaccg
    12541 gaggaatagg aggtgaaatt gctgctgaaa tttcttctgc ttgttttcaa tggctcgatg
    12601 cgcctgtgat gcgatgcggt agtcttgata ctgccgtgcc tatgtcaaat catttggagt
    12661 ggaattttct gccgcgtgat agatttaaac aaatgctcaa acaattagct gaatattaag
    12721 ctgtaaatta ttttttttca actgcccatt ttattgcttc gccaatagaa tattttcctg
    12781 caatagtcat aaactcactt gcagttgacc agattcctga tgagggttta tgctgtttta
    12841 attcttctac ttttatttca tcaaaccaaa cattgcctgt gccattcaaa attcctgtga
    12901 tgctcattgt ttgcgctccg tcaggaacag ccattgcaac agaataagtt ttccattttg
    12961 cttctgcatt cttaataaaa ccactgctct cagttaattt ttttcctgat gcatccctga
    13021 taattatatt aagaccggta ttcccattca gtgcttctgc tctgcacgaa taaattattt
    13081 tgtaattttt tatcgctgtg ttttcaataa ttatactctg actgaatgtg catgtagcct
    13141 gattttgatg tgccatttcc agacattgtt tacctgaaaa tggagaaatg ttactgactt
    13201 ttatctgacc agctccttta caatctgtta tccatccttt ctgagaagaa gtattagcat
    13261 cttcctcaaa gccactattt acaagtgtct gtgcctgaag ttgtattgtg caaatgcaca
    13321 aaataaatat cagtatttgc ctgaattgca tgattgcaaa taaagaaaat tcagtccaaa
    13381 cattcacaat atgcagttaa tattaatgca ttgtacgaaa gaaaagttta tttataaatt
    13441 ttacatttgc ttgcaagaag aatttattat gttccgttat tcattagcac tctttttttt
    13501 aatttccaca tcaacttcgc aggctcaaca caaaaaattg agcatggaag atgccatttt
    13561 aaatgcaaaa accaaccttg ctccggcaaa cctttcgcag ttaggatgga ttaaaaattc
    13621 aatttcatac tattatgttg acaaccgctt ggctgatgca acgctttttg taggcgatga
    13681 tggtatagta catgatgaga aaattatttc gctttcagaa attaatgaca gactaaaacg
    13741 tttaaaaaca gatacacttg cacgttttcc tgctgttgaa tggaagaatg ataaagtctt
    13801 tggattcttt ataggtaata aggaatatga atatgataaa acacaatatg ccattcgcaa
    13861 ttcaagaata ttgttgttac ccgacagtgc ttctcaaaag gatactgacc cggtaacaaa
    13921 gcgcatagct tatacaatta aaaataatct ttttgtgtat gatgcaggaa aaaattttac
    13981 agtagcagaa gatgcttacc cacatattgt ttatggcagt tctgttcatc gcaatgaatt
    14041 tggaataaca aagggaacat tttggtcacc aaatgggaat aaacttgcct tttacagaat
    14101 ggatgaaacc atggttaatg attatccgat tatagattgg aacaaaaaac cggcagaagt
    14161 aaatatgata aaatacccaa tggcaggtgg cacaagtcat caggtaactg ttggcgtatt
    14221 tgatatggct aataaaacag ttgtttatct aaaaacaggt gagcctaaag atcaatacct
    14281 gactaatata tcatggagtc ctgatgagaa gtatgtttat gttgctgttt taaatcgcga
    14341 tcagaaccac atgaaactga attgttattc ttcatcaaca ggtcattttg taaaaacact
    14401 tttcgaagaa aaagatgata agtatgtaga gccgcaaaca ccaatggcat ttgtgcccgg
    14461 tcatgatgat cagttcatct ggcaaagtca gcgtgatggc tacaatcatt tatatctcta
    14521 taatactgac gggaaattaa tcaggcagct tacgaaaggt gagtttatcg taacggaatt
    14581 taagggattt gataaaaaat cggagcgagc atattacatg tgcactgcat tgagtcccat
    14641 tgacagagat ttatattatg ttacacttgc agacggaaga atgaaaagag tttcgggcga
    14701 tagtggaatg catacttgtt attataatcc ggagaataat gaagtgcttg atgtattcag
    14761 caatgtcaac acaccacgta aaattaaact tattctgcct aatggctctg atgcaagaac
    14821 aatgctgacg gcagaaaatc cgttaagcga ttttaattta ggcggaatga aaatcagtac
    14881 actgcagagt gtttccggtg agttgcttta tacacgcacc tattatccac cggtaatgga
    14941 ttcaacaaaa aaatatcctg tgattattta tgtttatggt gggccacatt tacaactaat
    15001 tacaaattca tggcagggtg gacaaggtga cttgtggttc tattatctgg ctcaggaggg
    15061 atttattgtt tacacggttg atagcagagg tagtggtaac agaggaaagc agtttgagca
    15121 atccatattc cgaaactgtg gaaatgcgga gatggaagat caacttacgg gattaaaata
    15181 tttaaaatct ttaccttatg ttgattcaac acgaataggt gttgatggat ggagttatgg
    15241 cggttttatg actatatcac tgatgacgag gtttcccgga ttgtttaaag tggctgttgc
    15301 cggtggacca gtaattgact ggagtatgta tgaaattatg tacacagagc gttatatgga
    15361 tacgcctgct acaaaccctg agggctatga aaaaagtaat ttattgaatt atgtaaaaaa
    15421 tatcaacggt aagttaatgc tcattcacga cacaaatgat gaaacggttg tttggcaaca
    15481 tagtttgatg tatttgaaaa aagctgttga tctcggaaag caagttgatt actttgttta
    15541 tcccggacat ccacataatg ttcgcggtaa agatcgtgtg catctaatga ataaaattac
    15601 agattacttt aaacaaaatc tttgaaaaca atatttaatt aatgagtaag aaaacaattt
    15661 ttaatctttt actatgtttc tgtgttgttt caattcatgc acaaaatcta aaacgttgtc
    15721 cgcagacaac atcaaaagaa gcagctaaga agaccgaaga agcagagaag ctattaaagt
    15781 cagttaaaaa ttatgaggaa ggtgttgaat tgtgtaatga ggctattgag gctgacagca
    15841 catttgcaaa accctatttg cttatgggtg atgctgcata caggaaaaaa gatttcaagt
    15901 taatggcaaa ggcttataaa ggccttatga atgtttgtcc tgatgcatct gaaaaagcaa
    15961 tctacagact tgcaacatat tattttgata caaagaagta tgaagaagct attcctgttt
    16021 acactacatt ccttgaactt tctccgtcag ataccaacgc tgctgacgat gccgaagtga
    16081 aattgttcag agcaaaagcg tttttacatc cagtagattt taaacctgtg ttggtaaaag
    16141 gattaagcac accagatcct gaatatctgc caataatctc tgctgataat gagttgtgtt
    16201 tttttacaag aagatttgag tatcaaagta aaaacatgct cacttcgacc agtgtagaaa
    16261 agtttatgtt aagtcataat ctaaatggca aatgggatac cggtacacca atggatttac
    16321 cttttaacaa acagaatact ggtaatgaag gtggtccaac tatttctctc gacaacctgc
    16381 acttgttttt tactgttaat gtaaagggca attttgatat ttatacttct gaccttacac
    16441 caaaaggatg gtctgaacca agaagcatag ggaaagcaaa tgacccaaag ttttgggatt
    16501 cacaaccatc actttcacca gataataaga cattatattt tgccagtttc agagattctg
    16561 ttcttcagac aagtgatatt tatgtgacta aaaaaaataa tggtgaatgg aatagtgcaa
    16621 atcctatcag tggaccaatc aatacttcgc gaaatgaaaa agcacctttt atgcatcccg
    16681 atggaaaaac actttatttc agtagtgatg gtcaccccgg catgggtgga tatgatattt
    16741 attattccaa gaaggatgca aacggtaact ggggaaaacc tgttaatctc ggatatccga
    16801 taaataccga tgcagatgaa gttggatttt ttgtaagtac tgatggtact aaaggttttt
    16861 ttgcaagtaa taaattcgga acagggggtt atgatattta ctcattcgac atgccggtgg
    16921 aagcaaggcc cgagaagatg gttttattca aaggccaact taaaaatgat gacaatgaag
    16981 tgcctgacag cgcccgtata gaaattcgga atatttcatc caaacaggtt gagcagatag
    17041 attacgatgc atacagtgga aaatatgcag gtgtattgca agctaattct gagtatttgt
    17101 taactattca aaaagatgat tatgcattta cttcaaaact tattgatgct gcaaaagttg
    17161 attcgcctgc agttactgtt gagttagatc tgagaaaaat aaaagaaggc gaagcatata
    17221 gtttaaatga tatacttttt gaaacagatt cttatcagat tacagataaa tcaaaagcag
    17281 taattcagga ttttgcaggc tttcttaaaa gtaatccaaa gttaaaggtt gcaatttatg
    17341 gtcatactga taattcagga aaagcagaag ataatttgtt gttgtcaaaa aacagggcga
    17401 aggctgttta tgattatttg attgctttag gcattgaagc aacacgactt tcgcataatg
    17461 gattcggaca aacaaaaccg gttgctgcaa atacgacaga aaaaggtaag tcactgaaca
    17521 gacgcacaga gtttgtagtg ttaaaaaaat agttttatgg agtttgttat tttggttgat
    17581 gaacaggata gcgaagttgg tgaggcagaa aaaatgcaga ctcataaact tggattatta
    17641 cacagagctt tttcagtatt aatttacaat tccaatggag aaatgctttt gcaacaaaga
    17701 gcagaaacaa aataccatag tggaggactt tggtcaaatg cttgttgtgg tcatccacgt
    17761 cccggtgaaa atatagagga tgccgcaaag agaagactta aagaggagtt gggctttaat
    17821 tgtgatctga agtttagctt taaatttctt tataaagttt cttttgaaaa tagtcttaaa
    17881 gagcatgaac tggatcatgt tttcacaggg agttatgaaa gtgaagtact tcctgataaa
    17941 agtgaagttc aagccttccg gtggataaca atgaataatc tgaagaatga aattacagaa
    18001 aatcctgata aattttctgt ttggtttctt aaaattattt cggagttgta attccttgtt
    18061 atttagctga taatattgaa ataaattacc gaatcatacg ttttgcttga atgcaaatcc
    18121 ttatttttgg ttcctatgaa tacattcaat ttcagatgca ggaattttat cctgtctttt
    18181 ttatttttca gtatgcctgt tctggcattt gcgcagtctg gtcaggaaaa acttgttcaa
    18241 ttaagtggtg taatagttac aggggatagt ttaagtccgg taccctacac aggcattatg
    18301 attaagtcga gcagtagagg aacagtgtct gattattatg gttttttttc aattgtagca
    18361 cacgttggtg atgagattga gttttcttct gtcggatttg caaaaactat ttttaaagta
    18421 cccgatacgc tcacagaaaa ccgttattct ttgattcaga ttcttaaccg tgatactgtt
    18481 ttacttaatc aggctgtaat ttatccatgg cctactcgcg aacaatttaa acaggctttt
    18541 ctcgatttaa aagttcctga tacagacatg gatcatgcac gaagaaatct tgctcttgaa
    18601 accatgcgcg aagagagcat aagaattgat atggatggaa gcatgaacag taaatttgta
    18661 tatcagaaac aacaatcaag gttatacaat acaggccaat tgccggtaaa caatttgcta
    18721 aatcctattg catgggcaaa gtttattcag gcctggaaaa gtggtgagtt taagtcaaag
    18781 tctaaagacg attaatgagg caacttatcg tttttctttc tttgttattt tgttgttatt
    18841 ctcttaacgg ccaagatgca actgctcgta ttcatggcaa aataacaggc acagaccgta
    18901 aacctttgcc tggtgttagt gttggcattg caggtacttc attaggtgta atttctgatg
    18961 aacatggtga atattcaatt actgttccat ctaatcgtga gctgagtatt gttttttcat
    19021 caatcggttt tgagcaggca acggtaaaat taattcttgc agaaggtgaa aagaagcaat
    19081 taaacagaac catgttacca tcaatagtgc ctctgcccaa tatcactatc atagaccctc
    19141 gtgaaagaag tggcacatta ataaaaattg atccaaaagc tgttgaggaa atcccaaatc
    19201 catcaggtaa tttcgagacc attttaaaaa caattggaag tggcgttgtt tcaaacaacg
    19261 aacttagttc cgaatattct gtgcgtggcg gaaactacga tgaaaatctg gtttatgtaa
    19321 atgatgttga aatttacaga ccacaattag ttcgctcagg actacaggaa ggtttaagtt
    19381 ttatcaatcc tgatcttgtt tcaggaatta aattttcagc cggtggtttt gatgcttttt
    19441 atggagataa aatgtcttca gcattggata taaaatacaa acgaccaaca gaatttgcgg
    19501 gaaaaattac ttatggtttg cttggtggag gtctgagcct tgaaggtaca agtaaaaaca
    19561 accgttttac atatttgata ggagcacgac aaaaatcaac gaaatattta ttgggtacac
    19621 ttgatacaaa aggaaactac aaacctgttt tttacgatgt gcaggcattg ttgacctatg
    19681 atgtgagtga aaaaactaca atttctttat taggtaattt ttcaaaaagc agatacaata
    19741 ttgttcctca aacacgaaca acagatttcg gtacagtgag tcaggcattg cgattcacgg
    19801 tttattttga cggaaaagaa gttgatgatt ttactactac cacaggagcc gtaattttca
    19861 gtcatagtcc gaagaaaaat ataaatctca agctcatagg ctctgtgttt aacacaaaag
    19921 aagaagaaac ttttgatgtt cttggtcagt attttctgga tgaacttgaa aaagatcctg
    19981 cctcacaaaa ttttggtgag gtaaaactca acctcggtgt aggttctttt ttgaatcatg
    20041 cacgcaattt tcttgacggg aaagtttatt cagcagaaca taaaggtaca tacaattatc
    20101 gccataatga attgttgtgg ggtattcggt ttcagcacga acagttcagc gatcagatta
    20161 aagaatggaa ttatattgac tcatcaggtt attcattacc acatccgcag gatagtgtag
    20221 gagggagtct cccatcgcag ccgcaattat tattgcaaga tgtcatcagt cgtgataaca
    20281 gcctttcaag taacagatta agcggctatc tgcagcataa tttctatttc agtaacacat
    20341 caaattttat tctcacaccc ggtattcgtt ttacgtggtg gagtttgaat catgttttaa
    20401 acatcagtcc acgtatagca cttgcctata aaccaagatg gaaacgcgat tttaacttcc
    20461 gtctttcagg agggttttat tatcaaccgc cattctacaa ggagttgcac aatcctgacg
    20521 gaagtttaaa tacatctttg aaatcacaac aatctattca tgttgttggc ggaacagact
    20581 attatttcaa agcgtggggt cgcgatttta aatttgttac agaagcttat tacaaatggt
    20641 tgaatgacct tattccttac aaagttgata atgttcgaat acggtatttg ggtaaaaata
    20701 attccaaagg ttatgcaaca ggaattgatt ttcgtatcaa tggccagttt gttaatgaag
    20761 cagagtcatg ggcaagttta tcacttttaa aaacagaaga agatattcag gatgatgttt
    20821 attatatcag attgaatgca cgtggcgata ccatcagagg atacaccatt gacaaaaagg
    20881 ctgttgacag tattcaggtt atacccggat atttaccacg attaaccgat cagcgactta
    20941 cattcagcat atttttttct gactatcttc caaaatttcc aacatttaaa atgcacatga
    21001 cccttgtttt cggaacagga tttcctgtag gcccgcccgg aaataatcgt tatacagaca
    21061 ttaaccgtta tcctttttac agacgtgttg atattgggtt cagtaaaata tttattgacg
    21121 aaaacagacc ggcaaaaagt cgttttgctg cgttcaataa attgaaagcg ctttcacttt
    21181 caattgaagt gtttaatctt tttcaggtta acaatgttgt ttcctatact tgggtgagtg
    21241 atgtttatgg tactaaatat ggcgtaccta attatcttac cggaagacaa ttaagtttac
    21301 gactcagcgc taagttctga ttacatacgt cacaagatta ttagcttttc acagtaaaaa
    21361 taattttttg attcctcatc tttaaatcat aagctgattt gtttatatac tcattgcgtt
    21421 ttgaagaatc ttttaaattt gaatttttaa atttttcagc ttatgcgtaa tcattccgaa
    21481 agcaggttcc ttaccgaacg acagtcgcga attggcgaat ttctatttac tctcaaagta
    21541 gtaagagaat ttataaaagg attcagaaca ttgcattttg ttggaccttg tgttgcagtg
    21601 tttggctcag cacgatttaa agaagagcat cactattatc agttaggccg tgaagtaggg
    21661 gcaaatgtca gcagattggg atttacagtg atgaccggtg gcggtcccgg tgttatggaa
    21721 gcagctaaca gaggggcaaa agaagcagga ggtttttctg tgggttgcaa catcattctt
    21781 cctcgcgaac agcaacccaa taagtatctt gacaaaatgg ttgaatttca tcattttttt
    21841 gtcagaaaag ttttgatgtt caaatactcc tatgcttttg tggttttgcc tggaggtgtt
    21901 ggcacaattg acgaaatgtt tgaggcttta accttgattc aaacaaaaaa agtaagtgat
    21961 ttccctgtag tacttatggg taaaaattac tggcagcctg taattgatca gctcaaagta
    22021 tttgatgagc ataagacagc cttgcactct gagttagatt ctttgctgat tacagatagt
    22081 ttagatgaaa tgacagaata cctgcgcgtg aaggctattg agagatttaa gttgtctaaa
    22141 gccatgaaat tcaaccgctc gaaatggttt cttgaagaat gagcctgact gttggcaatt
    22201 attcaataaa aactttcgca attcatttct atcttatatt tttatcctgt tatttttcag
    22261 agtatttaca ggactaaatg aaagtattcc gcctttgctt ttttattttt atttcttcaa
    22321 tgctgcaagc gcaggatatt catttttcac aattctataa ttctcctctc acacttaacg
    22381 ctgcaaatac aggattgtat gaaggcgatt atcgttttgc aaatacttat agaagtcagt
    22441 ggtcgaaact tgacccgggt tacagaacca atgctttttc atttgacaaa acactttata
    22501 ccggagcaga tcagattagc ggaggctttt tgttcattta cgaccgttcg ggaattaatc
    22561 gtttgcaaag tattcgctta ggactatctg ctgcttatca tcgcacgttt ggagtacaca
    22621 gtttaagtgc agggtttcag tggaattata tttttaaatc tattgacaga agtaaaatta
    22681 ccatgcctga gcagtttaat atgaacaccg gttattttga tccatcaatg cctaccggtg
    22741 aaatgttgct tgacgagaaa cgttctttcg gtgatattaa tgcagggtta gtttatcagg
    22801 ttgaattaaa taaacatttt attcaggcag gtgcttcatt ttttcatctt aacaaaccgg
    22861 gtgatacttt ctttaacagc agtaataaat tacctgttcg gacatgttta accgtttctg
    22921 ataagattgg aattggtaat gattatcagt tacacactcg attattgatg atgcaacagg
    22981 taaaagccaa tgagtatgta gccggagtta cacttgtaaa acaacttaat aacggagaat
    23041 caaagtttac agaagttaat ggtggtttat atgtacgtac atcacccggt aacggcactg
    23101 acgcagtaat acctgtaatt ggcattaaat acgatttgtt tgatgtggca ctgagttatg
    23161 acatcaatat ttcaactctc catacagcaa ctgcaagccg gggtggattt gaaatatctc
    23221 ttatttacac tgcattaagt tcacgtttgt ttaaaataaa aataccctgt gaacgctatt
    23281 aaaacaagag ctatcctgct gctgatattg ttaatcagtt ctgttcattt atatgcacag
    23341 cagaatgatg aattaaaaaa atatggcaca gcacaattaa aacgaatggg tagttatgct
    23401 gcaaagatta acgatttcag cagttctgca atgtactatg aagagtattg taagcgcaaa
    23461 cctactaatg agaaggtttg gtttctttat gctgaaagtc ttcgtaaatc caaagattac
    23521 cgaaaagctg aagatgctta catgaaagca tacaatctga atccaaaact aaatgcttta
    23581 gcgttgtatc attatgccaa tgttcaaaaa tctcttggta attataaaaa ggctgaagag
    23641 tattttacaa aatttaaaaa agaatataaa ggctctgata aaagcgttta taccaaatta
    23701 attaaaaata gtggtgatgc tgccaggttt gcagatagtc ttatgcatag cccttcaaaa
    23761 gttgctattg aacatttgcc agaagatatc aattcatcac atgtagaatc atcgccattt
    23821 tatattaacg attcaacaat tatttatggt tctctgaaaa cagatcaggt aacttttgat
    23881 tttaatccaa gagattcgag tgctgatgag cctttccgaa aaatttatgt tgccgtgcgt
    23941 gatagtgaga tatggaagga tgatggtgaa ctgcccggat tttataattc aaacagtgct
    24001 catacactca atggtgttta ttcgcaggat aagagtagat tttattttac gcgatgcaaa
    24061 agaaatcgtc agaacaaagt tatatgtgcc atctatgtaa gcttcctgaa caatggaata
    24121 tggacagaac ctgtttcttt aggtactgaa gtaaacgatc caaaatatac ttcaacacaa
    24181 cctgccattg ggcttaacac gcagaaaaaa ggagaggaga taatttattt tgtcagtgac
    24241 cgcgaaggtg gaaaaggagg ctatgatatt tggtacactt cttttgataa cagaagtaaa
    24301 acttacaaaa cgccagtcaa tcttggaact aaaattaata cttcaggtga tgaagtaaca
    24361 ccctatgttg atgcacaaac acaaacattt tatttcagct ctgatggatg gcagggatta
    24421 ggaggattag atatttttaa gtcaattggt ggaatgaaaa agtggactca tcctgtcaat
    24481 ttgggttatc ctgttaattc caattatgat gatttgtatt atgtgctatc accaagtaat
    24541 aaggacattg gcatgatagt atcgaacaga aatgtaactg ataatggcat gaataaaggc
    24601 ccttgttgtg atgatatttt tgagtttaat attttagatg ctgtaagatt aaatgttaaa
    24661 ggctttttat acgaaacaga ttcaacacaa aaaggaaaga agttggcaat gatgcctgtt
    24721 cgaagagcac gtattacttt ggaaatgaaa aatgctgaag attctacttt tattcctgtc
    24781 agcaccagta atacagatga aacaggtcac ttcaacctta ttgtttctcc tagacaggaa
    24841 taccgtatca cagcacgcaa ggaaggttat ctttatgaat cagtagcgtt gagcactatg
    24901 aataaaacac gttccggtga tatttcaatt gatatacaca ttcgttcaat tcctgaaaca
    24961 ccattactgc ttacaaacat ttattacgaa tttgcaagcc ctttgctaac tgcaaaagct
    25021 atggctgcat tagatacaac cttggtgatt tttatgcaaa gaaatgttga ccttggtgtt
    25081 gaaataaggt cgcatacaga tgccattggc tctgatgatg ccaacatgac tttgtcgcaa
    25141 aagcgggcag aaagtgtggt gaattattta gtagcaaaag gaattaatgc caacaggttg
    25201 cgtgcaaaag gatttggtga aacaatgcct gtagcaccaa acacaaaacc tgacggaaga
    25261 gataatccca aaggcaggca gttgaacagg agaacagaat ttaaaattct gggtaaaatg
    25321 cgtgatggtg taatccgcga agaaaccgat ttctgatttt attttttcca ggtagtataa
    25381 accgcatcgt gctttttacg attgggttct atacttcgca aaggcgcaca gggttttaat
    25441 gtagctgtta attcatgcgg taattgttgg taaagttcag tcccttttgt tttccaatga
    25501 agcatttcat cgcttacatc ttttataatt tttgccggat tgccaacaac gattttcctg
    25561 tcaggaacaa tcatgccatc aggcacaaaa cataaagcgc caactataca accatcgcca
    25621 attacagcat tgtccattat tacagcattc attcctatca aagaattttt gccaatggtg
    25681 gcaccatgaa ctatggcacc atgaccaatg tgtgcacctt gttttaaaat aactgtaaca
    25741 ccgggaaaca tgtgaatggt gcagttctcc tgaacattgc agccatcttc aataatgatt
    25801 gtgccccaat cgccacgaat ggcagcaccc ggagcaatat aaacttgttt accaataatt
    25861 acatcacctg taactgttgc ctgttcatga acaaaactac tgctgtccac tacaggtctg
    25921 aatccgttaa attcgtaaat catttgaata actgatgttt cttgtaacga tgcaaatgta
    25981 aagctaattg atgtgccaaa actttaacaa ttgttggtaa ctatctgtaa gtattaatct
    26041 gtcagaagtt tatttttgtg ctaaaaccat cacattcatg tcaaaatatt ttgcttcaat
    26101 cattcttgta tttatttttc tgacttcaac attaagggct caaacactga agagttttaa
    26161 taccgaccct tcaagttatc tgcttgattt gaaatcattt tttgaagaga caaataagaa
    26221 ggaagcagag aaaattatgg aggaattcaa gcctgttttt cttggtaagt ttaatgcaca
    26281 acaacaacag actattatta ccacaagtaa tcttatgttg aagaagcgca tgaaggcttt
    26341 tcctgatttt gttacttata catcggtgtt gaatgctttt gcttccagcg gacaagaggc
    26401 agctacattt acatcatgga attcttcttt tgaaaaagca atagcaaaac ttagtgtaag
    26461 gaaattaggt gactttcttg aaatatctca attgcttttt cgtaacaaca ccttgtatga
    26521 atcaagcgct gttgcatgga gtgcaagcaa taacaaattt tcatttggtt tcgattcttt
    26581 accgaaagtt actttttccg gaatgacttt gcgatgtttg ggcaagggtg atagctctgt
    26641 aatagaaggt acaaaaggag tttattatcc caacaacctt ttattttttg gcgatggggg
    26701 gacagtaaat tttactcgtg caggaataag tgtttcagaa gcaaatgcag ttgtaaaacg
    26761 ctatgctatc aatttaaaag gttcagaata ctcaatggat tctgttgctt ttacatataa
    26821 gaaatatttt gatcaggagc tgaaaggccg ttacattgat aaattgttgg caaacgtaaa
    26881 tgattccaat gctacttatc cgaggtttta ttcttatgct gctaatctcg acataaagaa
    26941 tatggttaaa gatgccgatt acaaaggtgg attttctttg caaggaagta aaatggtagg
    27001 ctcaggcaac agacgacagg atgcatcaat aacttttaat ctgaatggta aacctcagtt
    27061 gaaattgctt tcgcaaggat ttatttttcg tacagacagg attgtatctg taaatgcagc
    27121 tgctgtaatt tattgggaga aagattcaat ttatcatccc ggagttgagt ttaaatatat
    27181 ttatggtgat aaaactgtaa cgctgaccaa aaacggtcaa actgccatca atagcccgta
    27241 ttttgacagc tatcataaaa tggatttaga ttttgatcag ttggtatgga agattacaga
    27301 cccattgatg gacctaaaaa tgatttcagg tggaggagag agtaagctga aatttgagtc
    27361 tgtaaatttc ttttcacgac agcgttttga taaaattcag ggactgtctg aagtacatcc
    27421 gcttttcaaa ataaagcaat atgcagagga gcataatgta aaagttatca gtgtgccgga
    27481 gtttgcggaa tatttaaaac tcactgaaaa cactgtgcgc aatcaaattc tgcagttgtc
    27541 atcattagga tttatttctt atgatgccga tgccgataag tttattgtta aagacaaggt
    27601 gatgtactac ctcaatgcac gcaccggtaa ggttgattat gatgagttga aaattgaatc
    27661 tatgatttca ggattatcta atgcaaccat caacttgttg aattttgata ttaacatgcg
    27721 tggtgtaagc catgtattaa tcagcgattc gcaggaggta tatattcaac ctttggatca
    27781 ggaaatgact ctgaaaaaaa atcgtgacat tgttttcaat ggacgtgttc atgcaggaag
    27841 acttgatttt tatggtaaag agtttacttt taattatgcc gattttaata ttgatttaaa
    27901 gaatgtggac tcactgcggt ttaaagtgcc tgctgactct gttgatgaaa gaggaaaaaa
    27961 acgtttgatt ccaataaaaa gcattttgca gcagttgaat ggaaaacttt ctattgataa
    28021 cccaacgaat aagtcagggt ttgtacctaa tgaggaatat cccgtttttg tatgtacaaa
    28081 caagtcttat atttactatg attatcctga aatattcggt ggtgtttata agcgtgataa
    28141 gttttattat catctcgacc cattcagtat tgacagcctt aacacactta ccaaagaagg
    28201 acttgtattt gaaggaagta tggtgtctgc agatattttt cctgaatggc gacagcaact
    28261 tacagtgcag cgagattatt ctttaggatt tattaccaac acacctgatg ccggttttgc
    28321 agcttatagc ggaaaaggaa aatatcggga tcaaatcagc ttgagtcatg aaggtttcct
    28381 tggaaaagga gaagttgatt acctgagcag cacttcaaaa tcaaacaata ttatattctt
    28441 tcctgacagt atgcatgcag ttacacaaag ttttgcaata agaaaagagc aagttggtgc
    28501 tacccgtttt cctgatgtta agaatgaaga tgtgtttgtt aactggcgac ctaagagtga
    28561 taaaatgttt gtatttaaga gaagcaaaga ttttgcattg tttaatggtg aggcagttct
    28621 cgatggaaac ttagtgctga tgccttccgg aattaaggct aatggtataa ttcgttacca
    28681 acaatctcag ttaatcagca acaatcattt tattaaagaa catagctatg gttccgattc
    28741 tgcagaattt aaactcaata cagatgcagg agatttgctg gcattgcata cacagaatgt
    28801 aaaagccaat attgatcttg ataaacgtat tggggatttc agcagtcagg gtgaaaaagc
    28861 taaggttgag tttcctgtta atgaatatat ttcttacatc agagatttca aatggtatat
    28921 agataaatca gaacttgaat ttggcggctc tgatgcaact gccgacaaag caaatgagtt
    28981 tgtgtctgta aacagacagc aggattcttt acgatgggat gcacctttag caacttataa
    29041 cttaaaatcg tacttgctaa aaggatttaa ggtgaaagaa attttagtag cagatgcttc
    29101 aataattccg gatttaggga atgttgttgt agagcgtaat gcagtaatgc gaccattggt
    29161 taacagtaag gttattgcta atactacttc gcactatcac actatgctaa atacaaatat
    29221 tactattcag agtagaatga attattcagg agatggcaat tatgaatata tggatcagac
    29281 aaaggttaaa cataaaatta aacttacaca gattggagtg gacacttcaa aacagacttt
    29341 tgccattgga gaaattccag attcatcacg tttttttatt agtcctacaa ttcagtataa
    29401 aggaaagtta aggattaatg caccaaaaga aaatctctac ttcaatggat ttgcaatcgt
    29461 taatcatcac tgtaatactt ttgaacgcaa ttggtttggc tttgcaggcg acattgaccc
    29521 aaaaggggta aacattccta ttcaaagtcc ggctaacgaa agtttgcaga aactttattc
    29581 aggaatttat tttggcagcg acagcaccat gatgtatcct gcattcttat caatgaaaaa
    29641 acatagttca gatgcagaag ttatttctgc agaaggctat ctgacttttg ataataattt
    29701 gcagcagtat aaaatttccg gtaccattcc cggtaccgat tcaaccaagt cagcaagtgt
    29761 tattactaat acagtaattc ttgatgacaa gagatgtcag ttgattggtg agggccgaat
    29821 aaacctgact caggattttg gtcagctttc aatcacaaat tttggtatgg tcaactattc
    29881 aacagttaat gactctatag gctttgatgc agttatggca actgacttta tgttcaatga
    29941 tgatgcattg aaacaaatgg cagagatgtt taattctatt cctgcattgc aaccttttat
    30001 ggacgataga ccggcttata gaaatgcatt gactaacatg attggaaaaa ataaagcgga
    30061 aaagttattg aatgaaacag ggctgtacgg ttcggcacga agaattcctt ctgaattgca
    30121 gcatacgctt atgttgagtg agcttaaatt ttattataat aaagaacgtg catcttatat
    30181 ttctgtagga aatatcggtc ttggtgtcat aggaaagacc tatgtaggaa gaatggtaaa
    30241 aggcaaagtt gagattatgc gcagacgtag tggagatgta ttgaatattt atctggaagc
    30301 attgcctgat atgtggtact attttaatta tcagcgtggt gtattacaag cgatttcttc
    30361 tgatgaaaaa ttcaataagc caattgatga aatgaaacca gaaaagcgca tagcatcgga
    30421 gaaaggaggt cttgaaccct atcagtttat gcttgccacc gagaggaaaa agaatgagtt
    30481 cctgaaacga attaaggatg cagatttgta agatgtaaat tttagatggg ttgaatttat
    30541 taaaaccaat aattagaaat tatttaatgc aaagagaaca tcattatcag gcaaatatta
    30601 tttggacggg caacaaaggt tcaggtacag atagttattt aggttacgca agaagttata
    30661 ctgttaacat tgataataaa cccgaaattc aaggttcagc agacgctgca tttcatggag
    30721 ataaaacaaa atataatcct gaggaaatgt tgttgatagc attggcttcg tgtcacatgt
    30781 tatggtattt gcatttatgt tctgaagcag gagtggtagt tgtagaatat acggatagag
    30841 caacaggtct aatgacagaa acaccatcag gcaatggaaa atttgaatct gtaacattaa
    30901 acccaatcgt agtgattaga gatcagacaa aaaatgaaat tgccattgaa ttacataaga
    30961 aagcaaatga gttttgtttc atagcaaatt cagtaaactt taaagttaat cacaagcctg
    31021 ttttcaaatt gatgaaatga gcgtatttta acaggaaaag ctttttttga aaaagcaaaa
    31081 ttcctcttgc ggcaacatga aaatggaaac ggctttatga ttttttcata ctttagctcc
    31141 gttttggtat cattatagtt agcaaacaat ttaaataaaa atcttattca aatgaaaaac
    31201 acattcttta cagtggcatt tattctttgt gctttttttg tgaaagctca atcctcaaat
    31261 gttattctat tcaccgagaa tggtgaaaag ttttttgcta tcctaaatgg agttcggcag
    31321 aatacaagtc ccgaaaccaa tgttaaagta acagggctta atgcaaattt ctacaaacta
    31381 aaagtgatct ttgaagacac taagttggga gagaaggatt ttaatcttgc cttcaatccg
    31441 ggaatggaaa ccactttcag tattaagttt attgcaaaaa agaacagtta tgttttgcgc
    31501 ccattgagcg aagtgccttt agcacaagca ttgcctcctg cagctaacca aaccgttgtt
    31561 gcttatagtg aagtacctgc gccaccaccg gcagctacaa cggttactca tactacaaca
    31621 actacaacaa ccgatggaac acccatgccc ggtgacaatg tgaatgtaaa cattggtgta
    31681 aatatgaatg aagcaggtgg cggaataaat atgaatgttt ccggaggaac aacacaaact
    31741 actacacata ctaccacaac aacagttacg cataccacta catcagaacc aactgcacca
    31801 cctatagcac ctccaccccc tgcaccggtt gtttatttgc cgggatataa tggcccttat
    31861 ggatgtcctg ttccgatggc tcctcaggat ttttctgagc ttaaacaatc catatcttca
    31921 aaatcttttg aagacagcaa gatgactatt gcaaagcaag tgcttcgtaa taactgcctt
    31981 tttacctctc aggtgaaaga tttgatggga ttgttcactt ttgaagagtc caaactggac
    32041 tttgcaaagt atgcttatga ttacacctat gatattggaa attattttaa agttaacgat
    32101 atgtttacat tcgagtcgtc aattgatgat ttgaataatt acatcaatag taaacattag
    32161 aaaatatttt ttttaaagat agccgggaat atttgttacc cggctttttt tattaagaaa
    32221 acaatttaat ggttggggat ttttatccaa tcactatatg tcatttcaaa cttaatttct
    32281 tttgacctat gagttccgac ctctgtccag cctgcttttc ggtaaaattg ttctgctctt
    32341 gtattaaagg cagttcctaa ccagacggtt tgctttgttt gggaaaaata ccagtctaac
    32401 attgtcttat gcaaatgtcg cccgataccc attttctcaa attttggatc taaaaacagc
    32461 gcccaaatgt tattctcttt caagtcagcg attgcaaaac ctactatttg attttcaatt
    32521 tcgcaaaccc aacctttacc tctttcggaa atatattccg cgcaatcttt gtccgtcact
    32581 aaatcgggat tagataaagt gttttctttt acagaatttc tgactctttg aatctgtttt
    32641 atgtcgtcaa ttttagcttc cctgattacc atttatttat tatcaggttg aaagtttttt
    32701 tcatattaat tagtaaatgt tgtttctgtt tgaattgttg tggtttgaga actgacttaa
    32761 agattactga atgtactata caactgttgc aaataacttt ttgcagtaac ggtgtcggca
    32821 attgcgtaat atggtctttt gtaaataaat ttttgattgc tcaaatcata gacaaatgta
    32881 caactgtctg ttacccatcc ttcggcatca tccatgcata aataagcaaa gccatttcgt
    32941 tttcggttaa gtaaatcgtt gctccaatta aacatatcac tattaaaccc taactgatgt
    33001 aataaagtgg aagggatgtc gtgatgcgag cctgtgctgt aaacaatttt atttttataa
    33061 atatttttta caacatcacc tgccattacc attggaatgt gtctgatgta atgactgtag
    33121 tatgacctgt cacgaggtat tttgttgcca tggtcagcaa ctataacaaa taatgtattt
    33181 ttaaaccaag gttctttttt tgcagcggca agataattgc ccaaactgtg atctgcatac
    33241 catgctgaac ccttaaactg ctcgtttggg ttatctttcc caaaaggcga ctgaatagga
    33301 atgtcgtatg gctcgtgaga acttaatgtc atcacaacag aaaagaaagg ctcttttgct
    33361 tgatttaaat cgttcaggtt tttttcaaaa acaaactcat catgtgctcc ccatttggcg
    33421 ctcaattgct gttgtttaaa tttcttttta cctgtaattt gctcaaagcc acagttgact
    33481 aaataggtgt tcagatttga aaagtcaaga tcgcctccat aataaaatga agtgctgtaa
    33541 ccttgctttt tcaactcagc aggcaaggag ggaagcttag cagtcttatc cggaaaacgc
    33601 atgatggatt gattaggctg tgccggaaat ccactcaata ctgccacaaa tgcctgatcg
    33661 gtgcgtgcac cactactgta aatattttta aatgataatc catcgtgaat taatgaatct
    33721 attgtgggag aaacaccggt ttcaccaccc atagaaccta caacatcaac tgtaaaactc
    33781 tccaacatga tgatgacaat gttgggtttt ttattgttca gtacaggcat taaatcaatt
    33841 ggtgtttgaa ataatttctt tacatgttct tcagcaactg catcactata aaatttgtaa
    33901 tatgaagcat tttcactttt ggcctgattg agattgtgcc ccaaatgcca agcaggattt
    33961 gtagcgatgt gattaagtgt tacattctca ctgaaagcag cagcactctc atttacaggc
    34021 agcatttgca cgccaccacg aaacattatt acaagcattg cagcggaaac aataaattga
    34081 agtaatgatt gaataattgc aagtttgtta tcaaaaaaat gttttgtaaa ttttctgaat
    34141 acaaaccatg taagtataaa taaaagtata atacatatta ttcccacaat caattcaaaa
    34201 ttgttcactg acaaaaacat ctgccgccag tctgctaaaa aagataatgc tctatagttg
    34261 attagtacac cccatgaacg ataaaggaaa agattactta catgcaataa aattaaaaca
    34321 aacagaatta ttccattata aacaagaaac aactttttta taaaagtatt ttctgttaat
    34381 gcatggatta acattaaaag taaaggcagt gccgaaatgt atgcagttgc tgaaatgtct
    34441 agtctgatac cataaaacaa tgtggaaggt aattcattta gttgaatgtt tgcggcatta
    34501 tactgaagaa acaagaaaaa gatgattctg aaaatgccga agaacagcat ccagaaaatt
    34561 gacagcacaa gaaaatttct aatcaaacgc atgttaatca gccttaatta attttccatt
    34621 acctgttcct tcatgcacta cgctgtcggg attgccgaag taataaatgt cgcctacata
    34681 agaaatttta gcagtgagcc attgcttagc tctgacatat tctttgttag cacctttgtt
    34741 ttctgtaaaa gtaacctctg tattcaggtt gcttgcatcg gcaatgccat tgccgttatt
    34801 ataaaaataa tgtacaccca cataaccatg aaagacaaaa tcacccggtc ctgtatgaat
    34861 gttagttctc acagctccgg tgttaaaaag aaaagtcacc ttgcccgatg caccccaatt
    34921 gttgaacgta aactcattat gtcgaatggt gtccatcagg tcaatgtttc ctgaaccatt
    34981 tactgttagt tctgcaatat caggcatcca aactttgaca gtaaatttat tcttaaaact
    35041 tctaacccaa ttacatgcat tttcgtttct gatgtacagc atgctgtctt ttacttctgt
    35101 tgtaatgcct tcaatcaatt ttgaacctgc cattacttca acttgataat cataacccgg
    35161 ataaattttt acatctacat tgttgcttgc tttaattcgg ttaaatccgg ttatatttct
    35221 tatttcagat ttgtcatttc ctgtcccttt aaagcaatca aacatgtggt ctttctgaca
    35281 ggaacagaag acaagcaaca gaagaaaaca aaaccagtta atgatttttg ccataggcca
    35341 gaataatgtc ttttaccaaa cgatgtcgga caacatcacc tgcatcaaga gtgataaagt
    35401 caatgccttt aatgtttttc agaattttca ttgcctgaat caaccctgaa ggttgtttgt
    35461 taggtaagtc aatttgtgtt acatcgcctg tgacaataaa ttttgcagca ggccccattc
    35521 ttgtaagaaa catttttagc tgactctctg tagtattttg tgcttcgtca agaattacaa
    35581 atgcatggtc taatgttcgg ccacgcataa atgccaatgg tgctacctca ataactccgt
    35641 tttctataaa ggtctttaac ttctcacccg gaatcatatc aaacaatgca tcgtaaagcg
    35701 gacgcagata aggatcaatt ttttctttta agtcacctgg taaaaaacca agattttctc
    35761 ctgcttcaac agcaggacgt gtaagaacta ttctcttgat ttctttattc tttaatgcgc
    35821 gtactgccag tgctacggca gtataggttt ttccggtgcc tgcaggacct attgcaaaca
    35881 caatgtcgtt tttattgcaa ctgtcaacca ttttttgctg attggcagta cgtgctttta
    35941 caacaacacc atttggtcca aagaccaaaa catcactgcc atttccggat ggtggattta
    36001 ctaaagtatc agagttttgt ccgtcagcca ttaacttttc caatacctgt tcatcaaatt
    36061 ttccgtagtg atgaaaatat tcaatggcac caatgatttt ttttgccacc caatgaattt
    36121 gcttttcatc acccaaaatt ttaagctcat tacctctgac aatcagctta aaagcaggga
    36181 atttttttct taataaatct aaattactat cgttgatgcc aaaaaatgct acaggatttt
    36241 ctaaatcaac gggtatggtt aattcactca attttacaat agtttaatgt atgcaaacct
    36301 aatttttttt ggacacatca gcgattgttt ttcttaaaat tccggtaatc ttcactaaca
    36361 tcagaaagtt tataatttgt caatacagta agatgtgctt acagtagtac ggatattttt
    36421 gtgaaattaa ttccggcagt attcatgtca ttaattacac tcacaacaga ttggggacta
    36481 accgatcact atgtagcagc actaaagggc gaattgctga gcatgttgcc gggtgtgaat
    36541 attgttgaca taagccatca gattccggct tacgatttta ctaaggctgc ctttgttttt
    36601 aaaaatgcat tttacttttt tccgcctcaa acaattcatt taatctgtgt tgacaaacaa
    36661 tctaagaaaa cagaagacac acattcaggg tggatggtta ttcaatatgg agaaagcatt
    36721 attgtttgcc gcaataacgg tttttttacc ttggtgagta agcaagagcc attaaagtcg
    36781 gtttacattg aaggttcaga agaggtgagt gtggcacatg agaaaagttt tttagttacc
    36841 ctgctacatg atttggttca ccataaacct ttggaagaaa taggacagcc gataaaatct
    36901 ttttatcaat catcaatgtt tcagccatcg cacgatgcca acaccattca gggaacggtt
    36961 attcatattg atacttacgg caatgctatt accaatattg aaaaggaata ttttgagaat
    37021 gtagtaggag caaaacgatt tgaaattgat atgcctcgcc acggtgatgc aattactaaa
    37081 atatataatc gctatgatga tgttactccc ggacagttgg tagcgctgtt taatgcatcg
    37141 ggctatcttg aaattgcctt aaatcagggt gatatttcat tgttgtacgg acttgagtat
    37201 ggagataaaa tcagaatttt tctgcaatga taacacgtat tgtacgattg cccgttaaac
    37261 ctgatgaggg caaaaatttt gaacaactat ttgatacaat aaaagaaaaa attgcagcct
    37321 ttgatggatg tttgtcagtt gaattgcata aagatgtagc ttcgtctgat tattttacct
    37381 acagcctttg gcagagtgaa agccatttgc aagcctatct cgactcatct ttttttacag
    37441 acgtttggcc taatgctaag aaactgttgc atcaacgtgc caatgcatgg tcgcttgaga
    37501 aagttttgaa tgtttggaaa gttgctaagt tttgaaagtt agcaagtttg aaagtttaga
    37561 atgtttgaaa gttttgaatg tttgaaagtt ttgaatgttg agatttaaat tacactgaca
    37621 agggcggtgc gacatgacag gtgaaattaa ctgttgcttg aatgatagat gggcacaagt
    37681 ccgaagactt gtgccagagg ggtttagaat gttgaaattt aaattacact gacaagggtg
    37741 gtacgatctg agaggtgaaa ctgaatttgt tctattttca taaaaaaagt attaaccctt
    37801 caatgtgttt atattatttc aaagtcctct gaaatgcgag agactttgaa agtacggaaa
    37861 cccacatgca ctataccatg tgttacctgc tgggcttttt ccattgtttg ttaaatacat
    37921 attcaaccat ttcttcaata ttaactattt gtcttgttga tgcattcatc cgaatatata
    37981 gttctttctc aatgaattca ttttttttgt ttttaagaaa tattggtttg taactttcgg
    38041 taataactat tactaaaata tctttaccct ctactttctt aattgataaa tctactaatg
    38101 gctttatgga taagtcaaaa aaataggcca aaagtgagtc aaattctaat aatagcttat
    38161 ccttttcatt ttctccatga aacaatgaat aatcataatt ttctaatcct tgaatattac
    38221 cgttatcatt tactccaata aataaaactc cgccatttga atttagaaat ccgcaaatag
    38281 ccttggctat taaatattta acacctattc ctgcggcttg agttttaaaa ttataaagta
    38341 aacatggttt aaattcgact tgattacttt cgcctctttt aataatactt agtaaatcta
    38401 tgccctcatt aatatcagtt tcttctgtat cttgatttga atcataaatc tcattatacc
    38461 tttcaatagt atcccaaaca taaggtgttt ttagaattgt tctttttgct ccgccttctt
    38521 catcttctcc tgattctgca tagtctaatc cataatgata aactgtcgtt tttaattcac
    38581 caacatttat ttgataatat tcataaagag gaacttcttg catttcaagg ctatcaatta
    38641 aattttgagg ttctaaatca tacatggaag aagataccct gtgtatttca aactcagttt
    38701 caactttacc ctcaaattca aatgcatcaa ttacgttttt agctatgtcg aggtcattaa
    38761 caactaaata aacagagatt tcttctttat attggttata taccttttta aactcaaaat
    38821 aatcctcaga gttttctttt ttatcaagga tttcgtttat gaaagaaggt tgagtaatta
    38881 cgaaatttcc ttgattactt tttttaagtc tcccaatttg ttccaaaaaa gataaatatt
    38941 cattaaactc ttcaaaagca tttgctctgt tttctaatgg tgttttatca tcaaattttt
    39001 ttataaatga ttcatttcga acattatttt ttttatcaaa cgctaaaagt ttgacacgaa
    39061 cttcgtaatg aaaagggtat ttttttgtca ttttataaat ttaagaattc ttttaattca
    39121 gtgtattttt caatcttcga ttcgcttgta attgaattcc catgtggaaa agctatattg
    39181 taaaaagttg tattacaatt ttcaattaat ctcccaagtt ttcccgtatt tgattcagga
    39241 acagtgtacc ctaaatggct aataaatttc tttaaaacct taccatgaag cagtactgtt
    39301 gtctttggtt tatagttatt aataacattt ttcaatctta tcaggtcagc ttttttaggt
    39361 ttaattttag cactattact ttctgcaatt tcagttatta aatcagtgat tccaaaattc
    39421 caattttgat acttatattt attcgcgcca aaaatcaatt catctgcatt tgatttgtca
    39481 actagcttag ttaacaatat agattcgtaa agttgattcc caaaagcttg gtttacagac
    39541 aagtagtgtc cgttttcgct tgaacctttt gctggattta atccaacaaa taaaatgtca
    39601 agattttttc ttagaaacgg tatcaatgtc gattcttgca tagtcgtttt ttttcttagc
    39661 cttgcaggta acgtcaatct gtgaaaaaaa tatatccaca aacttattta aaaataagga
    39721 agtaatgtaa ttgtaatcag attaattatt tacaataaat gcctacgatt cccggaattg
    39781 accgccacca aatcatcttt accgacttag aaagacaaat ctcaaaagac aatgtaatcc
    39841 gttttataga tgcttttgta gataagatgg atttaaaaca attggacatc ctgtgagagt
    39901 ggacaccaaa ctaccctgtg gtagattttc ttcaaaaaac gaactttata taaacctatt
    39961 taagtctttc tatttttcag ttcaaaaaat ggttgcataa aatcagtttg tcttaaaagg
    40021 caaacaatgg cttttagagt agagtatagg attgtttttt acagaaaaag ccttctttca
    40081 cagcctgacg gttcggggct taacgaagtg gtggatttcg aagcacaaaa ctttcaattc
    40141 accacaaatg ttgatgtgag gtagaatatt cggttaacca cgttacccgc caatttcttg
    40201 taggtgatgt tataagttgt tttctatttg tcctttgtgt caattagttc aaaaatcaac
    40261 tttgtcaatg tcttcacgtc actatcaata gttcctttca tatatctctc gtaaacactt
    40321 aagttgtcag gtgggttgag attgagtgtc aagtcttttt ctaatgccaa cagtcttaaa
    40381 aactctcttt gtgttcgtcc gtttccatct ctgaatgggt gtaaataatt aacgttatct
    40441 aaaatttctg tcaatttctc tgataacatt tttttgttgt ctttcgggat tttcttgaaa
    40501 tcggcaatta acaggtcaat gtatctgaaa gcattgtcaa agtgtgaagt cgggaaaaat
    40561 tgtttcccat ctttacttat ttcaacaatt cgtttcttcc ctgcccaagc ataaatatct
    40621 tgaaacaagt gtctatgaat ttcaaaaagg ctatcaatac ctttgatttt tgtagggttt
    40681 tcttaaagtt cttgaagtcg ttttgttacc gcaccactct ccacaaaaag caaaacatca
    40741 gcgtctgtaa tatcttgtaa attccttaaa agtcctgtct tagggtcggt ataggtaaag
    40801 tcagggtcta tgtatttgta ggaattagac ataggctttt tgtttggcga atgccacaag
    40861 ttctgttaga gatatttttc cagtaacata gtcgcgtata atttctattc cttttggtgt
    40921 aggtttaaat ccttcaaaca tactgtttcc caaagcattt gcagtgcttt caagcgtttt
    40981 caagtctgaa aattttaccc ccattatggt caggttattt ctgtctattt ctattgtgtt
    41041 aaacatatct ttcagtatta aaatactttt caaatttaca aaatttttag gtcaaattgt
    41101 ttgttttctg gctctttaaa atggcttaca acgtcagttt tccgatagct atcggaatta
    41161 aaactaatca ttagctcaaa agtaaaaaga aaaatgattt aag
//
LOCUS       DAOOOL010000007        37062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_75_length_37062_cov_88.587078, whole genome shotgun sequence.
ACCESSION   DAOOOL010000007 DAOOOL010000000
VERSION     DAOOOL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 37062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37062
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75_length_37062_cov_88.587078"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKU82_01255"
     CDS             complement(<1..390)
                     /locus_tag="PKU82_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81529.1"
                     /translation="MEQFVNVIKTLEEGVQLPKGDFINTILHCLSKFNWEEDVLKELK
                     VKLGGDDATIKQLIEKLLDVALLEFYEDEVPPSLTRYHRQLLLFDSINPSVQFENNYL
                     AQKRLQETHVLILGIGGIGNFVATSLVA"
     gene            complement(491..661)
                     /locus_tag="PKU82_01260"
     CDS             complement(491..661)
                     /locus_tag="PKU82_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81530.1"
                     /translation="MITETLVLFISAIMSVNTPFKTVPSKENNYKIKSEQRSTLLNSQ
                     EIERKYGGWDGN"
     gene            998..1549
                     /locus_tag="PKU82_01265"
     CDS             998..1549
                     /locus_tag="PKU82_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOZ81531.1"
                     /translation="MMILGIVGGILLLLIVFLISIFNELTKERVLVQEAWSGIGAFLQ
                     QRLDAIPNLVEIVKGYAGHENKTLTDVIRARNESIAALTPEAQIEAAKNMSAAMLNFK
                     ALAENYPDLKANQNFIKLQEQLTELEEKINQSRRYYNGTVREYNQSIAVFPKNIVAGM
                     FGFNASAFFAEDTDAAKAPKISF"
     gene            1561..3675
                     /locus_tag="PKU82_01270"
     CDS             1561..3675
                     /locus_tag="PKU82_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOZ81532.1"
                     /translation="MKVFKLTLALLLLAVTSLYAISFTKYSDKLFSQEEYNTLIKKSA
                     LAVAQEMHFPERAFAEVYSIHKIEITERSIPFIRKKFLWLEKGYSTWPASESVASKIR
                     KEVYERELQRGVRDALYADQNYLNSIFTINESDRIISFHSDIKVSVNGKLTVVETIKI
                     YNGDGGSNDEIKRGIVRTFPTRYTTSLGFVSTVPFDFISATCDGKTENYKKESAENGV
                     RYYFGNADHFLDRGYYTYVLTYETAKQIIFHPDKDELYWNVNGTGWSFTSDKVSCKIT
                     FPEQTKIIENNCYTGVQGSAEKLCNYKITAPNVVEFSTQQLLNSNEGLTISTTIAKGV
                     LIAPDSTEKAFAAIKDNFILPALVAAILFLIGYNYKAWRKVGRDPEAGTIIPQFEPPQ
                     NFSPADCGFLITKSYKSHHFAASLVDCAVNRLVDIEVKEEGMIFKTPVYYFNRPASAT
                     NEDQILNLRNQWYGYDIERLYGQKAEKGTYNSTIASVYNGLESHLKDRMEFTKSKSGS
                     FKNLFTLNFNYIGWGILVLALSVVGMIIYFILFGGTPVLVLSSIGLILVGIIIQILFA
                     RWMQAYSVEGRKILDYVLGFKMYLETTEQKKFDTMNPPDLTIQLFEKYLPYAIALECE
                     NQWSERFEAIINKAIEGGYQPAYFNMHGSSLNMRTFTSGLSSGISNTIASASTPPSSS
                     SGGSGGGGSSGGGGGGGGGGGW"
     gene            3856..4656
                     /locus_tag="PKU82_01275"
     CDS             3856..4656
                     /locus_tag="PKU82_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81533.1"
                     /translation="MKNRLQIKSALVTLLSVFGIVKLNAQCSYIPATTSATDTISYTF
                     SGGSFQSYGCAPIDPTYWLSGNGKTVTISYVNPQNFPKFRVWGMNDDDSAMVTVNGNY
                     YALTAATASYAPKVVCGISPGPDGVVFANGKVVGANNNQIGNYSYSDVQLNATNVNTI
                     VVTGISGAGWGFAGSTVYCPYVSGVSSVENQKISVYPNPVSDLLMIETENNNPNTILL
                     YDVLGNICLEKTFANKAVINVSEFLHGVYFYELRNDKKTLRFGKFLRQ"
     gene            4718..4948
                     /locus_tag="PKU82_01280"
     CDS             4718..4948
                     /locus_tag="PKU82_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015697087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOZ81534.1"
                     /translation="MKCPNCTTTDLVMSERQGVEIDYCPSCRGVWLDRGELDKIIDKA
                     VSNDSSQQHYQKPQHYSDHKYKKKHWLHDLFD"
     gene            5058..5465
                     /locus_tag="PKU82_01285"
     CDS             5058..5465
                     /locus_tag="PKU82_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81535.1"
                     /translation="MNFKPFFLLLTLVVAQSCSLGKIDQQKGVDLTTQLLNDLKNENY
                     SDIDKYYMDSFNASEPTETKIEKFKRLREAMGPIVSFEMLLAEVKKEPVSNVNQLHIK
                     YKVVCAKVTALQTFILVNDEGQPKIIFQNVENI"
     gene            complement(5477..6463)
                     /locus_tag="PKU82_01290"
     CDS             complement(5477..6463)
                     /locus_tag="PKU82_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ81536.1"
                     /translation="MNKKFLNLIGVIAVITALASCVPARQYDEMKMRKATCDEENSKL
                     RSENTNYSTQNKELTSSVADLKREKEKLETDTTTLGTAQRRLTSLYNELNDSYEKLLR
                     NNDRMMKDKNDETKKVIGQLQMTQEELIKKQDELTAKEKKLNELTEELKKRESKVNEL
                     ESVLNKKDEDVNALRKAVSDALVGFENNGLTITKKNGKVYVSMEEKLLFASGSTVVDK
                     KGEEALKELGKVLEKNKDINVLIEGHTDNVPISGGTMKDNWDLSVLRATSVVRILIKN
                     SNIDPTRLTPAGRGPYLPVDTGNTAEARRKNRRIEIILTPKLDELFKVIGEN"
     gene            6626..7339
                     /locus_tag="PKU82_01295"
     CDS             6626..7339
                     /locus_tag="PKU82_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81537.1"
                     /translation="MATLNDNWITEAHIDFEYKKYMLLAYLQHVSESFTENKLYPPLS
                     ELMKHYRNVTALRDNKKQFYDSFPERLSSADFNNFKLMYEKIVADDSLMQEVESIILF
                     SIPQFEKHISEGKKIYDFIESKIKVSPVGIIPLQINEGYFFLSTTQNRETRVYEYQIT
                     IFENPDEKYRALCTQYICSYEKNLSQNFENIKSDLLAIRKAFPNPATYSIESELMLPL
                     EETFLPLAKRTLMKYVNAH"
     gene            7481..10948
                     /locus_tag="PKU82_01300"
     CDS             7481..10948
                     /locus_tag="PKU82_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81538.1"
                     /translation="MIRLPLVFFLFLSVTLGYGQSLSNLRTKTIAVNSDTLHLDSLSI
                     VPGSFHLADSALLDISCMKVLYEKGILIINKSCNAKISDSLKISYRVFPVNFSQPHSN
                     RNLSIIKQKYSGQYNPFAYDNSENNTQSIFNFDGLNKSGSISRGISFGNNQDVIVNSG
                     LNLQLSGTLSDRVEILAAITDNNIPIQPEGNTQQLQEFDKVFVQLSRDSTRLIAGDFE
                     ISKPYGYFMNMYKKAQGAMVSSSFFIDEKSKWVMKTTDAAAISKGKFARNVFNGMEAN
                     QGPYRLTGAENETYIIVLSGTEKIYLNGVLLTRGKENDYVIDYNTAQITFSARRLITK
                     DSRIVAEFQYSDKNYVRTLLYSSNTFESKWAKFHVNYFTEQDNKNQPLFQDLNEDKRQ
                     VLANAGDNLQNAYFLNADSVGFNRNEVLYQKDTVTIDGIHYNYFTYSIDSTRAFYRLG
                     FSNVGQGRGNYRLLNSNVNGKVYEWIAPVNGQPQGDYEPVRLLIAPRRQQLLTAGGDV
                     GISKNTNVSVEGAFSKNDVNLFSRKDKSNDAGYALHAAVNHSMPVFKQDSSWKWVSVV
                     SAELTDKNFRPLENYRDVEFIRDWNLTSSFDTLNETFLSIKTGLERNVAQNIFYQLKY
                     FTKGNQYKGLMNTISANYNWKKYIIQYNGSLLNTTGAEANTVFLRSYVDAGKSFNKLK
                     AGYRQENERNRKSASIGDSLMLNSFGHDKAGVYLASADTGRWTYKTEVSRRWDYLPST
                     NELKLSTTADQADADIAFIAGRGKKITLGSTYRTIQVNRKEITSQKKDESFLGKLEIA
                     LQFLRGGITSNTYYEVGAGQEQRQAYSFIKVAAGAGVFEWNDYNGNGIAELNEFEVAA
                     FQDRAEYIKIFTPTNDYVRIQTNQLSEVISVNPSAFIKNKQGKTRLINRFALQSAIRF
                     DNKLLSGKWADGLNPFGYNINDTSLLITNSTLRNTLFFNRSSSVFAADFNYDNISNKS
                     LLANGTEGRNLTSWLLNTRTYFKRVYGLTTALKYETKQNSSVFTNRNYEIAGYSIEPR
                     FSVQPGTVFRTTLIYKYQDKQNVIELANEHATITTIGAECKYSSFKNGVASLGMNIIS
                     IGYNADVNSPLAFEMLEGLKKGRNYTWEISLQKNLTGNLQINIGYNGRKPQGDKIIHT
                     ANVQARAIF"
     gene            complement(10954..11889)
                     /locus_tag="PKU82_01305"
     CDS             complement(10954..11889)
                     /locus_tag="PKU82_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81539.1"
                     /translation="MEESVNEQTKADNREQNSRRALVFIVITILLGVNGLLLYQFFDK
                     KSHLEEVTRTLDNTSAERDALSAELQQVKAEFEKINQENSSLQAQLLSKDEEIKSKMA
                     QIRRMIESGDASQLKAAKAELERLRSMNQVYVAQLDSMRNANVALASQNESLNDMITV
                     EKNKSTALALENSTLTKRVAAASVLRTTSIKASGVKYKSSGKELETNKASSAQKIKTC
                     FTILENTVATSGSKDIYIRILSPDGVVMTNNTETFNVAGQSSLYSLKESFDYENREMN
                     MCVYWEKGTAFSPGKYTVEAYCEGSLIASTPVVLK"
     gene            12096..12779
                     /locus_tag="PKU82_01310"
     CDS             12096..12779
                     /locus_tag="PKU82_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOZ81540.1"
                     /translation="MINDFFKLFFPDNCPGCGHSFLRGEKYVCLQCLYGLHKTQYHND
                     PENPIAKNFWGKTDFVAVTAVYFFQKGQVLQRLLHQLKYNGCKEIGIMLGRQMAMELF
                     QASAFRNADIIIPVPLHPGKLLQRGFNQSEMIALGMAEYWEKEVHTETLKRKKYTQSQ
                     THKARYERFENTESVFELDNIKSYEDKHVLLVDDVISTGSTIISCAEAFSGISGCKIS
                     MASVAAPHR"
     gene            12810..12989
                     /locus_tag="PKU82_01315"
                     /pseudo
     CDS             12810..12989
                     /locus_tag="PKU82_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
     gene            13013..13327
                     /locus_tag="PKU82_01320"
     CDS             13013..13327
                     /locus_tag="PKU82_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81541.1"
                     /translation="MNPPDLTIQLFEKYLPYAIALECENQWSERFEAIINKAIEGGYQ
                     PAYFNMHGSSLNMRTFTSGLSSGISNTIASASTPPSSSSGGSGGGGSSGGGGGGGGGG
                     GW"
     gene            13508..14308
                     /locus_tag="PKU82_01325"
     CDS             13508..14308
                     /locus_tag="PKU82_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81542.1"
                     /translation="MKNRLQIKSALVTLLSVFGIVKLNAQCSYIPATTSATDTISYTF
                     SGGSFQSYGCAPIDPTYWLSGNGKTVTISYVNPQNFPKFRVWGMNDDDSAMVTVNGNY
                     YALTAATASYAPKVVCGISPGPDGVVFANGKVVGANNNQIGNYSYSDVQLNATNVNTI
                     VVTGISGAGWGFAGSTVYCPYVSGVSSVENQKISVYPNPVSDLLMIETENNNPNTILL
                     YDVLGNICLEKTFANKAVINVSEFLHGVYFYELRNDKKTLRFGKFLRQ"
     gene            14370..14600
                     /locus_tag="PKU82_01330"
     CDS             14370..14600
                     /locus_tag="PKU82_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015697087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOZ81543.1"
                     /translation="MKCPNCTTTDLVMSERQGVEIDYCPSCRGVWLDRGELDKIIDKA
                     VSNDSSQQHYQKPQHYSDHKYKKKHWLHDLFD"
     gene            14710..15117
                     /locus_tag="PKU82_01335"
     CDS             14710..15117
                     /locus_tag="PKU82_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81544.1"
                     /translation="MNFKPFFLLLTLVVAQSCSLGKIDQQKGVDLTTQLLNDLKNENY
                     SDIDKYYMDSFNASEPTETKIEKFKRLREAMGPIVSFEMLLAEVKKEPVSNVNQLHIK
                     YKVVCAKVTALQTFILVNDEGQPKIIFQNVENI"
     gene            complement(15129..16115)
                     /locus_tag="PKU82_01340"
     CDS             complement(15129..16115)
                     /locus_tag="PKU82_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ81545.1"
                     /translation="MNKKFLNLIGVIAVITALASCVPARQYDEMKMRKATCDEENSKL
                     RSENTNYSTQNKELTSSVADLKREKEKLETDTTTLGTAQRRLTSLYNELNDSYEKLLR
                     NNDRMMKDKNDETKKVIGQLQMTQEELIKKQDELTAKEKKLNELTEELKKRESKVNEL
                     ESVLNKKDEDVNALRKAVSDALVGFENNGLTITKKNGKVYVSMEEKLLFASGSTVVDK
                     KGEEALKELGKVLEKNKDINVLIEGHTDNVPISGGTMKDNWDLSVLRATSVVRILIKN
                     SNIDPTRLTPAGRGPYLPVDTGNTAEARRKNRRIEIILTPKLDELFKVIGEN"
     gene            16278..16991
                     /locus_tag="PKU82_01345"
     CDS             16278..16991
                     /locus_tag="PKU82_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81546.1"
                     /translation="MATLNDNWITEAHIDFEYKKYMLLAYLQHVSESFTENKLYPPLS
                     ELMKHYRNVTALRDNKKQFYDSFPERLSSADFNNFKLMYEKIVADDSLMQEVESIILF
                     SIPQFEKHISEGKKIYDFIESKIKVSPVGIIPLQINEGYFFLSTTQNRETRVYEYQIT
                     IFENPDEKYRALCTQYICSYEKNLSQNFENIKSDLLAIRKAFPNPATYSIESELMLPL
                     EETFLPLAKRTLMKYVNAH"
     gene            17133..20600
                     /locus_tag="PKU82_01350"
     CDS             17133..20600
                     /locus_tag="PKU82_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81547.1"
                     /translation="MIRLPLVFFLFLSVTLGYGQSLSNLRTKTIAVNSDTLHLDSLSI
                     VPGSFHLADSALLDISCMKVLYEKGILIINKSCNAKISDSLKISYRVFPVNFSQPHSN
                     RNLSIIKQKYSGQYNPFAYDNSENNTQSIFNFDGLNKSGSISRGISFGNNQDVIVNSG
                     LNLQLSGTLSDRVEILAAITDNNIPIQPEGNTQQLQEFDKVFVQLSRDSTRLIAGDFE
                     ISKPYGYFMNMYKKAQGAMVSSSFFIDEKSKWVMKTTDAAAISKGKFARNVFNGMEAN
                     QGPYRLTGAENETYIIVLSGTEKIYLNGVLLTRGKENDYVIDYNTAQITFSARRLITK
                     DSRIVAEFQYSDKNYVRTLLYSSNTFESKWAKFHVNYFTEQDNKNQPLFQDLNEDKRQ
                     VLANAGDNLQNAYFLNADSVGFNRNEVLYQKDTVTIDGIHYNYFTYSIDSTRAFYRLG
                     FSNVGQGRGNYRLLNSNVNGKVYEWIAPVNGQPQGDYEPVRLLIAPRRQQLLTAGGDV
                     GISKNTNVSVEGAFSKNDVNLFSRKDKSNDAGYALHAAVNHSMPVFKQDSSWKWVSVV
                     SAELTDKNFRPLENYRDVEFIRDWNLTSSFDTLNETFLSIKTGLERNVAQNIFYQLKY
                     FTKGNQYKGLMNTISANYNWKKYIIQYNGSLLNTTGAEANTVFLRSYVDAGKSFNKLK
                     AGYRQENERNRKSASIGDSLMLNSFGHDKAGVYLASADTGRWTYKTEVSRRWDYLPST
                     NELKLSTTADQADADIAFIAGRGKKITLGSTYRTIQVNRKEITSQKKDESFLGKLEIA
                     LQFLRGGITSNTYYEVGAGQEQRQAYSFIKVAAGAGVFEWNDYNGNGIAELNEFEVAA
                     FQDRAEYIKIFTPTNDYVRIQTNQLSEVISVNPSAFIKNKQGKTRLINRFALQSAIRF
                     DNKLLSGKWADGLNPFGYNINDTSLLITNSTLRNTLFFNRSSSVFAADFNYDNISNKS
                     LLANGTEGRNLTSWLLNTRTYFKRVYGLTTALKYETKQNSSVFTNRNYEIAGYSIEPR
                     FSVQPGTVFRTTLIYKYQDKQNVIELANEHATITTIGAECKYSSFKNGVASLGMNIIS
                     IGYNADVNSPLAFEMLEGLKKGRNYTWEISLQKNLTGNLQINIGYNGRKPQGDKIIHT
                     ANVQARAIF"
     gene            complement(20606..21541)
                     /locus_tag="PKU82_01355"
     CDS             complement(20606..21541)
                     /locus_tag="PKU82_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81548.1"
                     /translation="MEESVNEQTKADNREQNSRRALVFIVITILLGVNGLLLYQFFDK
                     KSHLEEVTRTLDNTSAERDALSAELQQVKAEFEKINQENSSLQAQLLSKDEEIKSKMA
                     QIRRMIESGDASQLKAAKAELERLRSMNQVYVAQLDSMRNANVALASQNESLNDMITV
                     EKNKSTALALENSTLTKRVAAASVLRTTSIKASGVKYKSSGKELETNKASSAQKIKTC
                     FTILENTVATSGSKDIYIRILSPDGVVMTNNTETFNVAGQSSLYSLKESFDYENREMN
                     MCVYWEKGTAFSPGKYTVEAYCEGSLIASTPVVLK"
     gene            21748..22431
                     /locus_tag="PKU82_01360"
     CDS             21748..22431
                     /locus_tag="PKU82_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOZ81549.1"
                     /translation="MINDFFKLFFPDNCPGCGHSFLRGEKYVCLQCLYGLHKTQYHND
                     PENPIAKNFWGKTDFVAVTAVYFFQKGQVLQRLLHQLKYNGCKEIGIMLGRQMAMELF
                     QASAFRNADIIIPVPLHPGKLLQRGFNQSEMIALGMAEYWEKEVHTETLKRKKYTQSQ
                     THKARYERFENTESVFELDNIKSYEDKHVLLVDDVISTGSTIISCAEAFSGISGCKIS
                     MASVAAPHR"
     gene            22462..22968
                     /locus_tag="PKU82_01365"
     CDS             22462..22968
                     /locus_tag="PKU82_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOZ81550.1"
                     /translation="MSESIVIDTTLSKQEKYAQLLPQAEALIKGETDAVANAANLCAM
                     LKQAFGFFWVGFYFVKQHEGKQQLILGPFQGPVACTRIELGKGVCGTAWQQKETIVVP
                     DVNNFDGHIACSSLSRSEIVVPILKHDVVAVLDVDSEHLNHFDETDKYYLEKISSLYS
                     ETFSYATE"
     gene            22955..24574
                     /locus_tag="PKU82_01370"
     CDS             22955..24574
                     /locus_tag="PKU82_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HOZ81551.1"
                     /translation="MLLNKINSFNRTFLLPAVLLLFGACASVVAPTGGKKDETPPKLK
                     EVIPPEQTVNFNSEKVVFTFDEYIQLLDKDKQLVISPIIEPAPTFDVKGKKLTITFKK
                     KLKENTTYTINFGNAVADLHESNPLAGFRYVFSTGTYVDTLFVKGNVVFAENLKSEKG
                     ILVMLYTSEDDSLPYNNPPAYFTRTDESGNFSIKNIARGPYKIFALKDADNNYKFNQS
                     DEWIAFDKTPITGGDSTPINLRMFKEAEKKVYVKEAKSFNAFAFSVLMNRPVGVLNIE
                     PLQTSDNIKLISRSKTNDTLMVWLKDSISDTLSYKLSGENNWTDTLTISKPAKALRGK
                     LKQSISLLSSTNIYPGNDVLLKTAIPVASVNKEKILLMHDSTTSQPFEIVFSDSASSN
                     ITIKSVTIEKNNWNLTLLPGAVTDIYGNKNDTAKLSFHTNAFTDYGSVKIKVDGLSGS
                     NYILQLVDEKDMVISEKYIGGNGSYLFEKMLPAKCRVRIINDADGNKSFTTGNYLLKQ
                     LPELVYYYKETLTLRANWDIEINWIIKEIKQ"
     gene            24571..24990
                     /locus_tag="PKU82_01375"
     CDS             24571..24990
                     /locus_tag="PKU82_01375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOZ81552.1"
                     /translation="MTEQQRIDSGKAPEPVGLYPHARRVGNLLFLSGVGPREKGSKKI
                     PGVEQDASGNIVQYDIAAQCHAVFKNVRYILEDSGSSWDKIIDVTVFLTNMKDDFPVY
                     NKLWAEYFKENPPCRTTVEINCLPTPIAIELKVIATI"
     gene            24993..25988
                     /locus_tag="PKU82_01380"
     CDS             24993..25988
                     /locus_tag="PKU82_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ81553.1"
                     /translation="MYLITGASGLVGSQLVFDLISMGRPVRILLRKSSSTALIKKKFK
                     SSPELFDKIKITYGDVLDIFSIEDALQDCTHVFHCAAMVSFNPQKRKEMYKVNTEGTA
                     NVVNACLEKGNIKLGFVSSVATLGRGTQQMINENDWFNQSEKNSYYAISKYAAEREVW
                     RGITEGLDAVIINPSVILGDGNWRSDSSSLFNTLYSGLKFYTQGVSGFVDVKDVSRSL
                     ISLTESKISGERFIISSENKTYEWLFKTMAKGLNINPPTIKAGMFLSQLAWRLEWLKS
                     VFTGKPSLITNETAYTSQQIYNYDNSKITEQLNYRFIPIEETVERVCKLFIAQKQ"
     gene            complement(25969..26457)
                     /locus_tag="PKU82_01385"
     CDS             complement(25969..26457)
                     /locus_tag="PKU82_01385"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOZ81554.1"
                     /translation="MSGNLFFRYANYNKWANQRITNAIVSAGEVAADIEMKSSFNTIR
                     KTVYHIYDSQHTWIMRIQNKPYSWPPSKDFKGDLNEFSKLLIESSQEWINYISNLNDQ
                     DFKKSISYKNTKGIDHQTPLEEIIMHCFNHSTYHRGQLVTMLRQAGVNSFEPMDFIAF
                     VR"
     gene            26534..27052
                     /locus_tag="PKU82_01390"
     CDS             26534..27052
                     /locus_tag="PKU82_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOZ81555.1"
                     /translation="MIIKPPSSQQLSEYYRGYLKYLPEEDMLQTLRIATDEMESMLEK
                     IPANKETFRYAPGKWMLKEVIGHLCDTERILTYRALCFARNDKTSFPGFDENEYVANS
                     NFKNRTMQSIGIEYLIVREASLALFNSLDETDFDRIGMANQTNTSARHMLYFCIVHQR
                     HHFNIIRERYYK"
     gene            complement(27060..28217)
                     /locus_tag="PKU82_01395"
     CDS             complement(27060..28217)
                     /locus_tag="PKU82_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ81556.1"
                     /translation="MNIYTQKQQWKLLLLASAILIGLLSLWYTNNLVKKLSEEERKKV
                     QLWAEASKQLANISVEAGDISFSLMVLQDNNTVPAILTDESGNILSHINLDTAKVNDS
                     QYVQEMLLQMKKRHEPIEISFANNQKNYIYYEDSGLLYQLRYYPYFQLAIIALFLLVS
                     YLAFSTSRKAEQNQVWVGMAKETAHQLGTPLSSLMAWIEYFKSTSQDQNHVAELEKDV
                     ARLNTITERFSKIGSAPVLRKEDVYMVIENAISYIRSRTSSKVIFSLNTAQPHQVLAP
                     LNIPLFEWVLENLFKNAVDAMDGEGKIDVMITDQLQFVYIDITDSGKGIPKSKFKTVF
                     KPGYTSKTRGWGLGLSLSKRIVEEYHNGHIFVKSSEPNKGTTFRIVLRKQV"
     gene            28399..28926
                     /locus_tag="PKU82_01400"
     CDS             28399..28926
                     /locus_tag="PKU82_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ81557.1"
                     /translation="MLNEQIIQGCKDKQGKAQHQLYELVAGRMMGVCLRYAKDDEEAK
                     DLLQEGFIKVFQQIDAFRGEGSFEGWIKKIFINTALESIRKRKLLTQPLEDEEDASDN
                     FLIQENKLEAKELLQMIRKLPDGYRVVFNLYAIEGFNHREIAQQLNITEGTSKSQYAR
                     ARQFLQKMLTEEKVK"
     gene            28947..29864
                     /locus_tag="PKU82_01405"
     CDS             28947..29864
                     /locus_tag="PKU82_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ81558.1"
                     /translation="MKEDKNIEQIFKDAFSNFEAPVDASLWQGIENSLPANPAPLTKV
                     SFLAKQAAYLKITSAVIAVATAVTAVLYFSNKNEKDTAQNVAQINKETTKAVEPATQQ
                     GVVLNNTETASKSEKINSSEEPKTKKSATVEPVKTELSDKVVENKSTQINSIPATETE
                     AILPPPQTDKQIISGPKTNTTAEPIKDTEVKKTEMKKPEAKTSVSAESKILPLPGLGN
                     MVFTPNGDGIGDWFEFDDSNLSDLSVKIYSLKGYKLVSEWHTHGGRWDGNLQNGNPAE
                     EGIYLVMIQAKGMDQTNYKSTIKLQLRRN"
     gene            29901..33188
                     /locus_tag="PKU82_01410"
     CDS             29901..33188
                     /locus_tag="PKU82_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ81559.1"
                     /translation="MSKILARLTALMLVATMLVPVSSKASHLAAGDIYYTYTGTPNTF
                     LITLRLYRDCAGITMSTSETVCYTSASCNISQSITVNLVPGSGQQIPPSPCVPSAGPT
                     TCQGGTAYGIEEYLYQAVLVMPAQCIDWKFQYETCCRNGNITTLNNAAGMGFFLETTM
                     NNLNYPTNSSPHFNTIPVTQFCVNNQFYFDQGATDPDNDSITYVLINAQDASGFCPYT
                     AFDLQYNAPYSGVYPISSANGVTMNLLTGTIAFLPNLLQNGVIAVRCFEYDRITGLLK
                     SIGKREIQINIVSTCTVVTPGFDSAQVASGVNIVIDGINNVTCDDTTFYIQLNPPVQC
                     GSIVPSDIRFLDPSGLPNPVTSAVGVNCVNGLTDSIMVTVLNPIPTGTSYIFTKIGND
                     GNTFLSECGSPMNEFDTLTINLYDSVPYTFDVTDTLDCTFDSLFIVSNQLIDCFTISA
                     DGSDFTLVDANGTPITITGATCLGTNGGHFGTTFLITTVPTTNAAGPLTLIVNTGSDL
                     NTIANGCGTMVLQDDTLGNIQVRNNITLSLGPDVTVCASDPAPVLDAGNHPGATFDWL
                     LNGTSTGQTSQTITPTASGTYIVNVQSSASCFGSDTVVVNLIPSPSVNLGTDVTLCAN
                     DPFPTLDAGNPGATYQWYLNDTLIAGATSQQFTPTTAGVYFVEVSTGATCLGRDTINI
                     SQVLQLNVSVNDLSYCSGNAVTLQSTSANSGVNYAWTLNGNPISGQTGTSVPVTGSGV
                     YAVTISTGSCSAIDSAIVTEVEQPTASLSNISQCDNVTLPVLNPGYVPSAGTNSVFSW
                     TLNSNPVGGNDQTLNTATTGAGNYNVTITNTSNGFTCTATASMTLTINPTPAVEIGNN
                     VTQCAGATPATLATANGTGNTYQWSLNNNIIVGANDTSITATSAGTYSVLVTSAGGCT
                     NTDNVVVSINALPTPEIHDELQKLDSVLFCATQNPAPVLSLTSNVFIQSYNWTLDGNN
                     LSTNANVTVAAIGEYQVTVIDSNGCSNTDVIRVIEAPCDLEIFNVITPNGDGKNDVFE
                     VKNLMDFKTRKLTVFNRWGKKVYSADPYNNDWDGGNQAAGVYYFVLEYDNGIKSGDKK
                     GSFNIIR"
     gene            33269..34222
                     /gene="metF"
                     /locus_tag="PKU82_01415"
     CDS             33269..34222
                     /gene="metF"
                     /locus_tag="PKU82_01415"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOZ81560.1"
                     /translation="MKVTEHFKNSKKTLFSIEILPPLKGKSIQSIFNTIDPLMEFKPP
                     FIDVTYHREEFVYKKREGGYLEKISIRKRPGTVGICAAIMNHYKVDAVPHIICGGFSR
                     EETENALIDLNFLGIDNVLLLRGDSEKHESHFTPEPGGHAYALELVEQVVNMNVGTFL
                     DDDLKGVEPTKFCIGVAGYPEKHFEAPNLNSDISFLKKKVDAGAQYIVTQMFYDNQKY
                     FNFVDKCRKAGINVPIIPGLKPISNKKQTAILPKTFFIDIPDDLIQEIEKCKSDDAVK
                     QAGIDWAVTQAKELIKFGVPCLHFYTMGNPENIRKIASQLF"
     gene            34295..35182
                     /locus_tag="PKU82_01420"
     CDS             34295..35182
                     /locus_tag="PKU82_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOZ81561.1"
                     /translation="MNRFSIKDIEILTGIKPHTIRIWEQRYGIIKPQRTATNIRYYTE
                     EDLKLMLNVSLLNKMGFKISKITCLKPNEIEHKVLSLVEQANDADINLSALLNCMVRL
                     DEDTFEHLLNKYLETNGLESTMTRILFPFLYRIGLLWQTGSITPAYEHFFTNLVRQKI
                     IAAIDKQVLSPLPDAKRFLLFLPPNEPHELGLLYAHYVIRQRGHRSVYMGLNLPIDHI
                     ICAHECCQPDFIVTTLTSCIKINAIEEFIVQISKTFSKSKILLSGAQAVNRSLHLPQN
                     VQVISDFDDFIQYIENRVN"
     gene            35246..36637
                     /locus_tag="PKU82_01425"
     CDS             35246..36637
                     /locus_tag="PKU82_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ81562.1"
                     /translation="MFIQQLYTSCLAEAAYYIESDGQAAIIDPIRETTPYLSLSEQRH
                     ARITHIFETHFHADFISGHIDLANKTGAKIVYGPDAHTNYTVHNAKDGEEFTIGKLTI
                     KAIHTPGHTLESTCYLLLDENKNPHCIFTGDTLFVGDVGRPDLLDGSGISKEFLADKM
                     FDSLNSKIKTLPDDVIVYPAHGPGSACGKNIGKETWSTIGQQKQSNYYLKEKDKASFI
                     KNLTTGILPPPMYFFEDARINKQGYRPIEEVIVRNAKPLALEDFKNEVNKGTLILDTR
                     NADDFEKGFVPGSVNIGLNGQYANWVGTLIPIQQPIVLVTENGKEEESVLRAARVGYE
                     NITGYLEGGIESWKNNGLEFHTISSVDAATFTNLLKTGSYTALDVRKPGEYESSHVKD
                     SVFVSLSDIPQHYNKLDKSITYLIFCGGGYRSMIAASLLKKNGFENVVNVYGGFGKIK
                     DCSISLETSQPVM"
     gene            36634..36933
                     /locus_tag="PKU82_01430"
     CDS             36634..36933
                     /locus_tag="PKU82_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ81563.1"
                     /translation="MNTVHEITTSNTNVLIDFYADWCEPCKWLNPVLEELTQQLPDLY
                     IHKINIDQFTEEAAAFEIKSVPVLILFVDGKQVWRMNGFKYAKELAKELKEFLKA"
BASE COUNT        12081 a         6447 c         7113 g        11421 t
ORIGIN      
        1 tgcaactaag gaagtggcaa caaaatttcc aataccgcca atacctaaaa tcagaacatg
       61 tgtttcttgt aatctttttt gagccaagta attgttttca aactgaacag acggattgat
      121 agaatcaaat aaaagtaatt gacggtggta gcgtgtcagt gatggtggga cttcatcttc
      181 ataaaattca agtaatgcaa catccaacaa tttttcgatc agttgcttaa ttgttgcatc
      241 atctccacca agttttactt tcaattcttt taacacatcc tcctcccaat tgaatttact
      301 taaacaatgt agaattgtgt taatgaaatc tcctttgggc aattggactc cttcttccaa
      361 tgtttttata acattaacaa attgttccat ttgtagaatg tgctggtaag gtttaatccg
      421 tatttttctt ttcacaaaat aaaaataaga agtaaaatgt tacccatagc gaaaaaggat
      481 tagagtgaat ttaattacca tcccatcctc catattttct ttctatttcc tgagaattta
      541 atagagttga tctttgttct gatttaattt tatagttatt ctcttttgat ggaacagttt
      601 taaaaggtgt gtttaccgac attatcgcac tgatgaataa tactaatgtt tctgttatca
      661 ttttcaatta ttttaaaagg tacaatttaa tttctatccc aaccacctcg aagatagcct
      721 ccagtgtttt cttttacaat ggtcttaact tctttagaag tttccttttt aatagttgta
      781 gttgattgtc caacattgtt atatggtatg atggatgcga gaaggaaaat aattggttct
      841 ataaacatat ttttattttt ttgataaggt aaaagtagta ttagaagcag ggtgtgtaaa
      901 taccacttta gtggtaagat ggcagttatt tgtttttaca attcagcacc atatcaatat
      961 ttgaatattt tttctacttt tacaaaaaac aacttatatg atgatacttg gcatagtcgg
     1021 tggcatactg ttgctactta tagttttcct tatttctatt tttaatgaac tcaccaaaga
     1081 gcgcgtgctt gtgcaggaag cgtggagtgg cattggtgca tttctgcaac aacgattaga
     1141 tgctattcca aatcttgtag aaatagtaaa gggatatgca ggtcatgaaa ataaaacgct
     1201 aacagacgta attcgtgccc gtaatgaatc tatcgctgca ctgacaccgg aagcgcagat
     1261 tgaagcagca aaaaatatga gtgctgccat gctcaatttc aaggcactgg cagaaaatta
     1321 ccctgacctg aaagccaatc agaattttat aaaactacaa gagcaactta cggagttgga
     1381 agaaaaaatc aatcagagcc gcagatatta taatggcact gtccgggaat ataatcagag
     1441 cattgcagtg tttcctaaga atattgttgc aggaatgttt ggctttaatg cttctgcgtt
     1501 ttttgctgaa gatacagatg ctgcaaaagc accaaaaatt tcattttaaa agtaactgct
     1561 gtgaaggtct ttaaactgac attagcacta ttgcttcttg cagtgacatc actatatgca
     1621 ataagtttta ctaaatattc cgataaactt ttttctcagg aagaatataa tacgctcatt
     1681 aagaagtcgg ctttggctgt tgcacaagaa atgcactttc ctgaacgtgc ttttgctgaa
     1741 gtatattcta tccataaaat agagattaca gagcgttcca ttccatttat cagaaaaaag
     1801 tttttatggc tcgaaaaggg ttatagcacc tggcctgcat cggaatctgt tgcaagtaaa
     1861 atacgaaaag aagtctatga aagggagtta caacgtggtg ttcgtgatgc gctgtatgcc
     1921 gatcagaatt atctgaactc aattttcacc atcaatgagt cagacagaat tatttctttt
     1981 cattctgata ttaaagtttc agtaaacggc aagctgacag ttgttgaaac aataaaaatt
     2041 tacaatggcg atggtggcag caacgatgaa ataaagcgtg gcatagtacg tactttccct
     2101 acacgttata caacatcatt gggatttgtc tctacagtac catttgattt tatttcagcc
     2161 acatgcgatg gtaaaactga aaactataaa aaagaaagtg cagaaaacgg tgtacgttac
     2221 tactttggca atgccgatca ttttcttgac agaggttatt acacttatgt tttgacttat
     2281 gaaacagcca agcagataat ttttcatcct gataaagatg agttgtattg gaatgttaac
     2341 ggaacaggat ggagttttac atcagataaa gtgagttgta aaataacttt tcctgagcaa
     2401 acaaaaatta ttgaaaacaa ttgttacacc ggtgtgcaag gctctgcgga gaaactatgt
     2461 aattataaaa taacagcacc aaatgttgtt gagttcagta cgcagcaatt actaaacagt
     2521 aatgaaggtc tgacgatttc aacaaccatc gcaaaaggag ttttaattgc tcctgactca
     2581 acagagaaag catttgcagc aattaaagac aactttatac ttccagcatt ggtggcggca
     2641 atactttttt taatcggata taactacaaa gcatggcgca aagttggtcg tgaccctgag
     2701 gcaggaacta ttattcctca gtttgagcca ccacaaaatt tttctcctgc cgattgcggt
     2761 ttcttaataa caaagagtta caagtcgcat cactttgccg catcattagt tgattgtgcc
     2821 gtgaacagat tggttgatat tgaagtcaaa gaagaaggaa tgatttttaa aactccggtt
     2881 tactatttca atagacccgc ttcagcaact aatgaagatc aaatactgaa tttaagaaat
     2941 caatggtatg gctatgatat tgaaagacta tatggacaaa aagcagaaaa aggaacttac
     3001 aattcaacca ttgcatcggt ttataatggt ttagaaagcc atttaaaaga tagaatggag
     3061 tttaccaaga gtaagtcagg cagttttaaa aatttgttta cactgaattt taattacatt
     3121 ggctggggaa tacttgtttt ggctttaagt gttgttggaa tgataattta ttttattctt
     3181 tttggtggaa caccggtttt agtgttatca tctattggtt taattctagt gggaataatt
     3241 attcaaattc tatttgcaag gtggatgcag gcctattctg ttgagggtcg gaaaatattg
     3301 gattatgtac ttggctttaa aatgtatctg gaaacaacag aacagaaaaa gtttgataca
     3361 atgaatccac ctgatttaac cattcagctt tttgagaagt atttgcctta tgccattgct
     3421 ttggaatgtg aaaatcaatg gtcagaaagg tttgaagcta tcattaacaa agctattgaa
     3481 ggaggctatc aaccggcata tttcaacatg cacggcagca gtttaaatat gcgcacattt
     3541 acttctggac tttcaagtgg tatcagtaat accattgcat cagcatcaac tccaccaagc
     3601 agcagcagtg gtggcagcgg aggtggaggt tcttccggag gcggaggtgg tggaggcgga
     3661 ggcggaggct ggtaacctca tgctgttttt tcacgtatta aggatgaaaa gtttaatacc
     3721 atttcttttg agaaagtgta gtgttgatta acttgcactt atcaaaagca tatcaaagct
     3781 aacttatatt taatcgtgca gttaaaaatt ttcagagtaa atcatttgat caaaatagta
     3841 aataaaaaac gatttatgaa aaacagatta caaattaaat cagcattagt aactttatta
     3901 tcggtatttg gtattgtaaa acttaatgcc cagtgtagct acattcctgc aacaacatct
     3961 gcaaccgaca ccatttctta tactttttcc ggtggctcat ttcaaagtta tggctgtgca
     4021 cctattgacc ctacctattg gttgtcggga aatggaaaaa ccgtaaccat tagttatgtg
     4081 aacccgcaga attttcctaa attcagagtg tgggggatga atgatgatga ctcggcaatg
     4141 gtgacagtta acggcaacta ctatgcatta actgcagcaa ctgcctcgta tgcgccaaag
     4201 gtggtgtgcg gtatttctcc gggacctgac ggagttgttt ttgccaatgg aaaggttgtt
     4261 ggtgccaata ataatcagat cggaaattat tcttattccg atgtgcagtt gaatgccact
     4321 aatgtgaaca ccattgttgt gacaggaatt agcggtgcag gatggggttt tgccggttca
     4381 acagtttatt gtccttatgt ttcaggtgtt tcatcagtag aaaatcaaaa aattagtgtt
     4441 tatccaaatc ctgtttctga tttgttgatg attgaaacag agaataacaa cccaaacaca
     4501 atattgcttt atgatgtttt aggtaatata tgtttagaaa aaacttttgc caataaagcg
     4561 gtgattaatg tcagtgaatt tttgcatggt gtgtattttt atgagttgag aaacgataaa
     4621 aaaacgctca ggtttggaaa atttttaaga caataaacat ttgaatgttt ttgttgttgt
     4681 aatacattag ctttgctgat aataataaca acgaaatatg aaatgtccta attgtacaac
     4741 aacagactta gtaatgtcag aacgccaggg cgttgaaatt gactactgcc catcgtgcag
     4801 aggtgtttgg ctcgacagag gtgaacttga taaaattatt gataaagctg tatcaaacga
     4861 tagtagccaa caacattatc agaagccgca acattacagc gaccataagt acaagaagaa
     4921 acattggcta cacgatttgt ttgattaatt agtagccaga agtattatta gttctttaaa
     4981 taatttgtgg ttataatttc caagacaaat tagatcagga gtatcaaata ttgataacag
     5041 taaagtaata tcacacaatg aattttaaac cattttttct gcttttaaca ttagtagtgg
     5101 cgcaaagttg ttccttagga aagattgatc agcaaaaagg tgttgaccta acaacacaac
     5161 tgctgaacga tttaaagaat gaaaactatt ccgacattga taagtactat atggattctt
     5221 ttaatgccag cgagccaaca gaaactaaaa tagaaaaatt taaaagactt cgtgaggcaa
     5281 tgggcccaat agtttctttc gaaatgttat tggctgaagt aaagaaagaa cctgtaagta
     5341 atgtcaacca actacacatt aaatacaaag ttgtctgtgc aaaagtaact gcccttcaga
     5401 cattcatttt agtaaatgat gaagggcagc ctaaaattat ttttcagaat gttgaaaata
     5461 tctgaaccgc taaaaattaa ttctctccta taactttaaa aagttcgtca agttttgggg
     5521 ttaagataat ttctatgcgt ctgttttttc tgcgtgcctc agcagtgtta ccggtatcaa
     5581 caggcaaata aggtcctctg cctgcaggag ttaatcttgt agggtcaata ttggagtttt
     5641 taataagaat acgaaccaca cttgtagcac gcaacacact taaatcccag ttgtctttca
     5701 tagttcctcc cgaaattggc acattgtcag tatgaccttc aatcaacacg ttgatgtctt
     5761 tgtttttttc aagcaccttg cccaattcct tcaatgcctc ctcacctttt ttatcaacca
     5821 cggtgcttcc tgaagcaaac aacaatttct cctccatgct cacatagact ttaccattct
     5881 ttttagtgat ggtaagtccg ttattttcaa aacctacaag ggcatcactc acagcttttc
     5941 tcaatgcatt aacatcttca tcttttttat tcaatacaga ctccagctca ttaacttttg
     6001 attcgcgttt tttaagttct tctgtcaact cattcagctt tttctctttt gcggtaagtt
     6061 catcctgttt ttttatcagc tcttcctgtg tcatctgtaa ctgaccgatt acttttttag
     6121 tttcatcatt cttatctttc atcatcctgt cgttattgcg aaggagcttt tcatacgagt
     6181 cgtttagttc attgtaaagt gatgtcagcc tgcgttgtgc agttcctaag gtagtggtgt
     6241 ctgtctctaa tttttctttt tcgcgtttta aatctgcaac agaagaagtg agctccttat
     6301 tttgggtaga ataattggtg ttttcactac gtagcttact gttctcttca tcacaggtgg
     6361 cttttcgcat tttcatctca tcatattgtc ttgccggaac acatgaagcc aatgcagtaa
     6421 tgactgcaat cacacctatt aaatttaaaa atttcttgtt cattttcgtt gtgtttaaat
     6481 ttattgaaac tttgtttctg aatattaagt aaaattataa tatagtgccc aaaattaagc
     6541 gtttgtcgct ttgagttttt gcaagaatag atgcatcagg tataattttt gaacaattaa
     6601 aacagacaaa aaaagtttaa atgttatggc aacactaaat gacaactgga ttacagaagc
     6661 acatatagac tttgagtaca aaaagtatat gctgctagcg tatttacaac atgtttcaga
     6721 gagctttact gaaaacaaac tctatccgcc attaagcgaa ctgatgaaac actatcgtaa
     6781 tgtaacggca ttgcgtgata ataaaaaaca attttatgat agttttccgg aacggctttc
     6841 ttctgccgat tttaacaact ttaaactcat gtacgaaaaa attgttgcag atgacagcct
     6901 gatgcaggaa gttgagagta tcattctatt cagtattcct cagtttgaaa aacatatttc
     6961 tgagggtaaa aaaatttatg attttataga atcgaaaatt aaagtcagtc cggttggtat
     7021 tattccattg cagattaatg agggttattt ctttctttca accacacaaa accgcgaaac
     7081 aagagtgtat gaatatcaga taaccatttt tgaaaatccg gatgagaagt atcgcgcact
     7141 ttgcacacag tatatttgtt cgtacgaaaa aaacctgtcg cagaatttcg aaaatatcaa
     7201 gagtgattta ttggccattc gcaaagcatt tccaaatccg gcaacttata gtatagagtc
     7261 tgaactaatg ctgccattgg aagaaacttt tctgcctttg gcaaaaagaa cattaatgaa
     7321 gtatgtgaat gcacattaac ttgtggtata accgactata aaaagaaagt attgctgttg
     7381 tttcagatac aagagaagat ttaagcctgt ttgttcaata tagtggcata acctgtcgtt
     7441 aacaagcaga tgcatttgct atttttagca cttcaaaagt atgattcgac tgccgcttgt
     7501 tttttttctt tttctgagtg ttacacttgg ctatggacag tcgctgagta atttacggac
     7561 caaaactatt gctgttaact cagatacact gcatcttgat tcgctttcca ttgtgcccgg
     7621 ctcatttcat ttggccgatt ctgctttatt ggacatatcg tgtatgaaag tgctttacga
     7681 aaaaggaatt ttgattatta ataaatcatg taatgcaaag atttcagact cactaaaaat
     7741 atcctatcgt gtttttccgg ttaatttcag tcaacctcac agtaaccgga acctgagtat
     7801 aatcaaacaa aaatattctg ggcagtacaa tccttttgct tatgacaact cggaaaacaa
     7861 cacacaaagt attttcaatt ttgacggatt aaataaaagc ggaagcattt caaggggcat
     7921 cagctttggc aacaatcagg atgttattgt caattcagga ttaaatcttc agttgtcggg
     7981 aacattaagc gacagggtag aaattcttgc agcaattacc gataacaata tccccattca
     8041 accggaaggt aacacacagc agttacagga atttgataaa gtttttgttc agctttcgcg
     8101 cgacagtaca cgattgattg ccggagattt tgagatttca aagccttacg gatattttat
     8161 gaatatgtat aaaaaagcac agggcgcaat ggtgtcatcg tcatttttta ttgatgaaaa
     8221 aagtaagtgg gtgatgaaaa ctaccgatgc cgcagctatt tcaaaaggga agtttgcacg
     8281 taatgtgttt aatggcatgg aagccaatca ggggccttac agacttacag gggcagagaa
     8341 tgaaacctac atcattgttt tgagtggcac agaaaaaatt tatctcaatg gtgttttgct
     8401 gacaagaggt aaggaaaacg attatgtgat tgactacaac actgcacaaa taacattttc
     8461 tgcaagacga cttatcacaa aagactcaag aatagtagca gagtttcagt attcagataa
     8521 aaattatgta cgtacacttc tgtattcctc aaacaccttt gagtcaaagt gggcaaagtt
     8581 tcatgtcaac tatttcacgg agcaggataa taaaaaccaa cccctgtttc aggacttgaa
     8641 tgaggataaa agacaagtgc ttgccaatgc cggtgacaat ttgcagaatg cctacttcct
     8701 caatgccgac agcgttgggt ttaaccgcaa tgaagtttta tatcaaaaag atactgtaac
     8761 aattgatggt attcattaca attattttac ctattccata gattcaacaa gggcattcta
     8821 tagattagga ttcagtaatg tagggcaagg aagaggtaat taccgtctgc taaacagcaa
     8881 tgtaaatggg aaggtatatg aatggattgc tccggtaaat ggtcagccac agggcgatta
     8941 tgaaccggtg agattattga tagcaccaag acgacaacaa ttgcttactg caggaggaga
     9001 tgttggtatc agtaaaaata ctaatgtttc tgttgaaggg gcattcagta aaaatgatgt
     9061 taatttattt tctcgaaaag acaaatctaa tgatgcagga tatgcactgc atgcagcagt
     9121 caatcattca atgcctgttt ttaaacaaga tagttcatgg aaatgggttt ctgtggtttc
     9181 tgctgaatta acagataaaa atttcagacc attggagaat tacagagatg ttgagtttat
     9241 acgcgactgg aacctgacat cttcatttga cacactgaat gaaactttct tgtctattaa
     9301 aaccggatta gaaaggaatg ttgcacagaa tattttttat caactgaaat attttacaaa
     9361 aggaaatcag tacaaaggat taatgaatac tatcagtgct aattacaatt ggaaaaaata
     9421 tattattcaa tataacggca gtttactcaa tacaaccggt gcagaggcaa acactgtttt
     9481 tttaagaagt tatgttgatg ccggaaaaag ttttaataaa cttaaagcag gatacagaca
     9541 ggagaatgaa cgtaatagaa aaagtgcttc gattggtgat agcctgatgc taaacagttt
     9601 tggtcatgat aaggcgggtg tttatttagc atctgcagat accggcaggt ggacttataa
     9661 aacagaggta tctcgcagat gggattatct gccaagtact aatgagctta aactttcaac
     9721 cactgccgat caggccgatg cagatattgc atttattgct ggaagaggaa agaaaattac
     9781 tttaggcagt acatacagaa caattcaggt aaacagaaaa gaaattacaa gtcagaaaaa
     9841 ggatgaaagc tttctgggaa aattagaaat agcattacag tttttaagag gtggaataac
     9901 aagcaatacc tattatgagg ttggggcagg gcaggaacaa agacaggctt actcatttat
     9961 taaagttgca gcaggggcgg gtgtttttga gtggaacgac tataacggta acggcattgc
    10021 cgaacttaat gaatttgaag tagctgcatt tcaggacaga gcagagtata taaaaatatt
    10081 tacgccaaca aacgattatg tcagaattca aaccaatcag ctaagtgaag taatcagtgt
    10141 caacccatcg gcattcataa aaaacaagca gggcaagacc agactgataa acaggtttgc
    10201 tttgcaatcg gcaatacgat ttgataacaa actgctttcc ggaaaatggg cagacggact
    10261 gaatcctttt ggttacaata ttaacgacac atcattgctc atcaccaact ctactttgcg
    10321 aaacacatta ttttttaatc gcagctcatc agtgtttgct gccgatttta attatgataa
    10381 catcagcaat aaaagtttgc ttgccaatgg cactgaagga agaaatttaa catcatggct
    10441 gttaaataca agaacatatt ttaaaagagt gtatggtcta acaacagcgt tgaaatatga
    10501 aaccaaacaa aacagttcgg tttttacaaa caggaattat gaaattgcag gctattccat
    10561 tgagccgcgt tttagcgtgc agccgggaac agttttcaga actacactca tctacaagta
    10621 tcaggacaaa caaaatgtaa ttgaattggc aaatgaacat gcaacaatca caaccatagg
    10681 tgcagagtgt aaatacagca gctttaaaaa tggtgtagca tctttaggaa tgaatattat
    10741 cagtattgga tataatgcag atgttaattc accattagct ttcgaaatgc ttgagggact
    10801 taagaaagga agaaactata catgggaaat aagccttcag aaaaatctta caggcaacct
    10861 gcaaatcaat attggatata acggacgcaa gccgcagggt gataaaatta ttcacactgc
    10921 caatgtgcag gcaagagcaa ttttctaaga aaattatttc agaacaacag gagtagatgc
    10981 aatcaggcta ccctcacaat acgcttcaac agtatatttt ccggggctaa aggcagttcc
    11041 tttttcccaa taaacacaca tattcatttc gcggttttcg tagtcaaaag attcttttaa
    11101 tgaataaagt gaagactgtc cggcaacgtt gaatgtttct gtgttgttgg tcatcaccac
    11161 accatcaggg ctaagtattc tgatgtaaat atctttagag cctgatgtgg caacagtgtt
    11221 ttcaagaatt gtaaagcagg tcttgatttt ttgtgccgat gatgctttgt tggtttctaa
    11281 ctccttgcca ctgctcttat attttactcc ggatgcttta attgaagtag tccttagtac
    11341 tgatgcagca gcaactcttt ttgttaatgt tgaattttca agtgctaatg cagtagattt
    11401 atttttttct actgtaatca tatcattcaa cgactcattt tgtgaagcta atgcaacatt
    11461 ggcatttcgc atggagtcta actgtgcaac atatacctga ttcattgatc tcagtcgctc
    11521 taactctgct tttgcagcct ttaactgtga agcatcaccc gactcaatca tccttctgat
    11581 ttgcgccatt ttgctcttaa tctcttcatc cttactaagc aactgagcct gcaagctgga
    11641 gttctcctga tttatttttt caaattcggc ttttacctgt tgcaactctg ctgacaaggc
    11701 atcgcgttca gcagaagtat tgtcaagagt tcttgtaact tcttccaaat gacttttttt
    11761 atcaaaaaac tgatatagca acaatccgtt aacccccaat agtatggtga ttacaataaa
    11821 tacgagtgct ctgcgtgagt tttgctcacg gttatctgct ttggtttgtt cattcactga
    11881 ttcttccatc tatctgaaat gggttgttaa acggtgcaaa ataatgtaat tagtatttac
    11941 aagcaagtaa aaaggatgaa atattctctg tttctacgta aaatgacaat tttgagtttc
    12001 cttggccacc gcttaatttg ctttttttat tcggtaaacc ttacattttt ttaaacaaaa
    12061 gcatatattt acttcttgat ttaaaaccgc cacttgtgat aaatgatttt tttaaattgt
    12121 tcttccccga caactgcccg ggctgtgggc atagtttttt gagaggtgaa aaatatgtgt
    12181 gtctgcaatg tttgtacgga cttcataaaa cacagtatca taacgacccg gaaaatccta
    12241 tagcgaaaaa tttctggggc aagacagatt ttgtagctgt cacggcagtt tattttttcc
    12301 agaaaggtca ggtgctgcaa cgacttttac atcagctaaa gtacaatgga tgcaaagaaa
    12361 taggcatcat gttaggcaga caaatggcta tggagttgtt tcaggcttca gcattcagaa
    12421 atgctgatat tattattcct gttccacttc acccgggaaa acttttgcaa agaggcttca
    12481 atcaaagtga aatgattgcc ctgggtatgg ctgaatattg ggagaaagaa gtgcacacag
    12541 aaactttgaa acgaaaaaag tacacacagt cgcaaacaca taaagcacgc tatgaaagat
    12601 ttgaaaatac agaaagtgta tttgagcttg acaatattaa gtcttatgaa gacaaacatg
    12661 ttttattggt tgacgatgta atctcaacag gctcaaccat aatttcatgt gcagaggctt
    12721 tttcaggcat tagcggatgt aaaatatcta tggcttctgt tgctgctcct catcgttgaa
    12781 tttctgatat ttgcacagaa gtttttagca tgtcagagtc catagttata gatacaacac
    12841 taagtaagca agaaaagtat gcacagcttt tgccgcaggc agaagcattg attaaaggag
    12901 agacagatgc tgttgccaat gctgccaatt tatgtgccat gctcaagcag gcttttggtt
    12961 ttttttgggt aggattttat tttgtgaaac agaacagaaa aagtttgata caatgaatcc
    13021 acctgattta accattcagc tttttgagaa gtatttgcct tatgccattg ctttggaatg
    13081 tgaaaatcaa tggtcagaaa ggtttgaagc tatcattaac aaagctattg aaggaggcta
    13141 tcaaccggca tatttcaaca tgcacggcag cagtttaaat atgcgcacat ttacttctgg
    13201 actttcaagt ggtatcagta ataccattgc atcagcatca actccaccaa gcagcagcag
    13261 tggtggcagc ggaggtggag gttcttccgg aggcggaggt ggtggaggcg gaggcggagg
    13321 ctggtaacct catgctgttt tttcacgtat taaggatgaa aagtttaata ccatttcttt
    13381 tgagaaagtg tagtgttgat taacttgcac ttatcaaaag catatcaaag ctaacttata
    13441 tttaatcgtg cagttaaaaa ttttcagagt aaatcatttg atcaaaatag taaataaaaa
    13501 acgatttatg aaaaacagat tacaaattaa atcagcatta gtaactttat tatcggtatt
    13561 tggtattgta aaacttaatg cccagtgtag ctacattcct gcaacaacat ctgcaaccga
    13621 caccatttct tatacttttt ccggtggctc atttcaaagt tatggctgtg cacctattga
    13681 ccctacctat tggttgtcgg gaaatggaaa aaccgtaacc attagttatg tgaacccgca
    13741 gaattttcct aaattcagag tgtgggggat gaatgatgat gactcggcaa tggtgacagt
    13801 taacggcaac tactatgcat taactgcagc aactgcctcg tatgcgccaa aggtggtgtg
    13861 cggtatttct ccgggacctg acggagttgt ttttgccaat ggaaaggttg ttggtgccaa
    13921 taataatcag atcggaaatt attcttattc cgatgtgcag ttgaatgcca ctaatgtgaa
    13981 caccattgtt gtgacaggaa ttagcggtgc aggatggggt tttgccggtt caacagttta
    14041 ttgtccttat gtttcaggtg tttcatcagt agaaaatcaa aaaattagtg tttatccaaa
    14101 tcctgtttct gatttgttga tgattgaaac agagaataac aacccaaaca caatattgct
    14161 ttatgatgtt ttaggtaata tatgtttaga aaaaactttt gccaataaag cggtgattaa
    14221 tgtcagtgaa tttttgcatg gtgtgtattt ttatgagttg agaaacgata aaaaaacgct
    14281 caggtttgga aaatttttaa gacaataaac atttgaatgt ttttgttgtt gtaatacatt
    14341 agctttgctg ataataataa caacgaaata tgaaatgtcc taattgtaca acaacagact
    14401 tagtaatgtc agaacgccag ggcgttgaaa ttgactactg cccatcgtgc agaggtgttt
    14461 ggctcgacag aggtgaactt gataaaatta ttgataaagc tgtatcaaac gatagtagcc
    14521 aacaacatta tcagaagccg caacattaca gcgaccataa gtacaagaag aaacattggc
    14581 tacacgattt gtttgattaa ttagtagcca gaagtattat tagttcttta aataatttgt
    14641 ggttataatt tccaagacaa attagatcag gagtatcaaa tattgataac agtaaagtaa
    14701 tatcacacaa tgaattttaa accatttttt ctgcttttaa cattagtagt ggcgcaaagt
    14761 tgttccttag gaaagattga tcagcaaaaa ggtgttgacc taacaacaca actgctgaac
    14821 gatttaaaga atgaaaacta ttccgacatt gataagtact atatggattc ttttaatgcc
    14881 agcgagccaa cagaaactaa aatagaaaaa tttaaaagac ttcgtgaggc aatgggccca
    14941 atagtttctt tcgaaatgtt attggctgaa gtaaagaaag aacctgtaag taatgtcaac
    15001 caactacaca ttaaatacaa agttgtctgt gcaaaagtaa ctgcccttca gacattcatt
    15061 ttagtaaatg atgaagggca gcctaaaatt atttttcaga atgttgaaaa tatctgaacc
    15121 gctaaaaatt aattctctcc tataacttta aaaagttcgt caagttttgg ggttaagata
    15181 atttctatgc gtctgttttt tctgcgtgcc tcagcagtgt taccggtatc aacaggcaaa
    15241 taaggtcctc tgcctgcagg agttaatctt gtagggtcaa tattggagtt tttaataaga
    15301 atacgaacca cacttgtagc acgcaacaca cttaaatccc agttgtcttt catagttcct
    15361 cccgaaattg gcacattgtc agtatgacct tcaatcaaca cgttgatgtc tttgtttttt
    15421 tcaagcacct tgcccaattc cttcaatgcc tcctcacctt ttttatcaac cacggtgctt
    15481 cctgaagcaa acaacaattt ctcctccatg ctcacataga ctttaccatt ctttttagtg
    15541 atggtaagtc cgttattttc aaaacctaca agggcatcac tcacagcttt tctcaatgca
    15601 ttaacatctt catctttttt attcaataca gactccagct cattaacttt tgattcgcgt
    15661 tttttaagtt cttctgtcaa ctcattcagc tttttctctt ttgcggtaag ttcatcctgt
    15721 ttttttatca gctcttcctg tgtcatctgt aactgaccga ttactttttt agtttcatca
    15781 ttcttatctt tcatcatcct gtcgttattg cgaaggagct tttcatacga gtcgtttagt
    15841 tcattgtaaa gtgatgtcag cctgcgttgt gcagttccta aggtagtggt gtctgtctct
    15901 aatttttctt tttcgcgttt taaatctgca acagaagaag tgagctcctt attttgggta
    15961 gaataattgg tgttttcact acgtagctta ctgttctctt catcacaggt ggcttttcgc
    16021 attttcatct catcatattg tcttgccgga acacatgaag ccaatgcagt aatgactgca
    16081 atcacaccta ttaaatttaa aaatttcttg ttcattttcg ttgtgtttaa atttattgaa
    16141 actttgtttc tgaatattaa gtaaaattat aatatagtgc ccaaaattaa gcgtttgtcg
    16201 ctttgagttt ttgcaagaat agatgcatca ggtataattt ttgaacaatt aaaacagaca
    16261 aaaaaagttt aaatgttatg gcaacactaa atgacaactg gattacagaa gcacatatag
    16321 actttgagta caaaaagtat atgctgctag cgtatttaca acatgtttca gagagcttta
    16381 ctgaaaacaa actctatccg ccattaagcg aactgatgaa acactatcgt aatgtaacgg
    16441 cattgcgtga taataaaaaa caattttatg atagttttcc ggaacggctt tcttctgccg
    16501 attttaacaa ctttaaactc atgtacgaaa aaattgttgc agatgacagc ctgatgcagg
    16561 aagttgagag tatcattcta ttcagtattc ctcagtttga aaaacatatt tctgagggta
    16621 aaaaaattta tgattttata gaatcgaaaa ttaaagtcag tccggttggt attattccat
    16681 tgcagattaa tgagggttat ttctttcttt caaccacaca aaaccgcgaa acaagagtgt
    16741 atgaatatca gataaccatt tttgaaaatc cggatgagaa gtatcgcgca ctttgcacac
    16801 agtatatttg ttcgtacgaa aaaaacctgt cgcagaattt cgaaaatatc aagagtgatt
    16861 tattggccat tcgcaaagca tttccaaatc cggcaactta tagtatagag tctgaactaa
    16921 tgctgccatt ggaagaaact tttctgcctt tggcaaaaag aacattaatg aagtatgtga
    16981 atgcacatta acttgtggta taaccgacta taaaaagaaa gtattgctgt tgtttcagat
    17041 acaagagaag atttaagcct gtttgttcaa tatagtggca taacctgtcg ttaacaagca
    17101 gatgcatttg ctatttttag cacttcaaaa gtatgattcg actgccgctt gttttttttc
    17161 tttttctgag tgttacactt ggctatggac agtcgctgag taatttacgg accaaaacta
    17221 ttgctgttaa ctcagataca ctgcatcttg attcgctttc cattgtgccc ggctcatttc
    17281 atttggccga ttctgcttta ttggacatat cgtgtatgaa agtgctttac gaaaaaggaa
    17341 ttttgattat taataaatca tgtaatgcaa agatttcaga ctcactaaaa atatcctatc
    17401 gtgtttttcc ggttaatttc agtcaacctc acagtaaccg gaacctgagt ataatcaaac
    17461 aaaaatattc tgggcagtac aatccttttg cttatgacaa ctcggaaaac aacacacaaa
    17521 gtattttcaa ttttgacgga ttaaataaaa gcggaagcat ttcaaggggc atcagctttg
    17581 gcaacaatca ggatgttatt gtcaattcag gattaaatct tcagttgtcg ggaacattaa
    17641 gcgacagggt agaaattctt gcagcaatta ccgataacaa tatccccatt caaccggaag
    17701 gtaacacaca gcagttacag gaatttgata aagtttttgt tcagctttcg cgcgacagta
    17761 cacgattgat tgccggagat tttgagattt caaagcctta cggatatttt atgaatatgt
    17821 ataaaaaagc acagggcgca atggtgtcat cgtcattttt tattgatgaa aaaagtaagt
    17881 gggtgatgaa aactaccgat gccgcagcta tttcaaaagg gaagtttgca cgtaatgtgt
    17941 ttaatggcat ggaagccaat caggggcctt acagacttac aggggcagag aatgaaacct
    18001 acatcattgt tttgagtggc acagaaaaaa tttatctcaa tggtgttttg ctgacaagag
    18061 gtaaggaaaa cgattatgtg attgactaca acactgcaca aataacattt tctgcaagac
    18121 gacttatcac aaaagactca agaatagtag cagagtttca gtattcagat aaaaattatg
    18181 tacgtacact tctgtattcc tcaaacacct ttgagtcaaa gtgggcaaag tttcatgtca
    18241 actatttcac ggagcaggat aataaaaacc aacccctgtt tcaggacttg aatgaggata
    18301 aaagacaagt gcttgccaat gccggtgaca atttgcagaa tgcctacttc ctcaatgccg
    18361 acagcgttgg gtttaaccgc aatgaagttt tatatcaaaa agatactgta acaattgatg
    18421 gtattcatta caattatttt acctattcca tagattcaac aagggcattc tatagattag
    18481 gattcagtaa tgtagggcaa ggaagaggta attaccgtct gctaaacagc aatgtaaatg
    18541 ggaaggtata tgaatggatt gctccggtaa atggtcagcc acagggcgat tatgaaccgg
    18601 tgagattatt gatagcacca agacgacaac aattgcttac tgcaggagga gatgttggta
    18661 tcagtaaaaa tactaatgtt tctgttgaag gggcattcag taaaaatgat gttaatttat
    18721 tttctcgaaa agacaaatct aatgatgcag gatatgcact gcatgcagca gtcaatcatt
    18781 caatgcctgt ttttaaacaa gatagttcat ggaaatgggt ttctgtggtt tctgctgaat
    18841 taacagataa aaatttcaga ccattggaga attacagaga tgttgagttt atacgcgact
    18901 ggaacctgac atcttcattt gacacactga atgaaacttt cttgtctatt aaaaccggat
    18961 tagaaaggaa tgttgcacag aatatttttt atcaactgaa atattttaca aaaggaaatc
    19021 agtacaaagg attaatgaat actatcagtg ctaattacaa ttggaaaaaa tatattattc
    19081 aatataacgg cagtttactc aatacaaccg gtgcagaggc aaacactgtt tttttaagaa
    19141 gttatgttga tgccggaaaa agttttaata aacttaaagc aggatacaga caggagaatg
    19201 aacgtaatag aaaaagtgct tcgattggtg atagcctgat gctaaacagt tttggtcatg
    19261 ataaggcggg tgtttattta gcatctgcag ataccggcag gtggacttat aaaacagagg
    19321 tatctcgcag atgggattat ctgccaagta ctaatgagct taaactttca accactgccg
    19381 atcaggccga tgcagatatt gcatttattg ctggaagagg aaagaaaatt actttaggca
    19441 gtacatacag aacaattcag gtaaacagaa aagaaattac aagtcagaaa aaggatgaaa
    19501 gctttctggg aaaattagaa atagcattac agtttttaag aggtggaata acaagcaata
    19561 cctattatga ggttggggca gggcaggaac aaagacaggc ttactcattt attaaagttg
    19621 cagcaggggc gggtgttttt gagtggaacg actataacgg taacggcatt gccgaactta
    19681 atgaatttga agtagctgca tttcaggaca gagcagagta tataaaaata tttacgccaa
    19741 caaacgatta tgtcagaatt caaaccaatc agctaagtga agtaatcagt gtcaacccat
    19801 cggcattcat aaaaaacaag cagggcaaga ccagactgat aaacaggttt gctttgcaat
    19861 cggcaatacg atttgataac aaactgcttt ccggaaaatg ggcagacgga ctgaatcctt
    19921 ttggttacaa tattaacgac acatcattgc tcatcaccaa ctctactttg cgaaacacat
    19981 tattttttaa tcgcagctca tcagtgtttg ctgccgattt taattatgat aacatcagca
    20041 ataaaagttt gcttgccaat ggcactgaag gaagaaattt aacatcatgg ctgttaaata
    20101 caagaacata ttttaaaaga gtgtatggtc taacaacagc gttgaaatat gaaaccaaac
    20161 aaaacagttc ggtttttaca aacaggaatt atgaaattgc aggctattcc attgagccgc
    20221 gttttagcgt gcagccggga acagttttca gaactacact catctacaag tatcaggaca
    20281 aacaaaatgt aattgaattg gcaaatgaac atgcaacaat cacaaccata ggtgcagagt
    20341 gtaaatacag cagctttaaa aatggtgtag catctttagg aatgaatatt atcagtattg
    20401 gatataatgc agatgttaat tcaccattag ctttcgaaat gcttgaggga cttaagaaag
    20461 gaagaaacta tacatgggaa ataagccttc agaaaaatct tacaggcaac ctgcaaatca
    20521 atattggata taacggacgc aagccgcagg gtgataaaat tattcacact gccaatgtgc
    20581 aggcaagagc aattttctaa gaaaattatt tcagaacaac aggagtagat gcaatcaggc
    20641 taccctcaca atacgcttca acagtatatt ttccggggct aaaggcagtt cctttttccc
    20701 aataaacaca catattcatt tcgcggtttt cgtagtcaaa agattctttt aatgaataaa
    20761 gtgaagactg tccggcaacg ttgaatgttt ctgtgttgtt ggtcatcacc acaccatcag
    20821 ggctaagtat tctgatgtaa atatctttag agcctgatgt ggcaacagtg ttttcaagaa
    20881 ttgtaaagca ggtcttgatt ttttgtgccg atgatgcttt gttggtttct aactccttgc
    20941 cactgctctt atattttact ccggatgctt taattgaagt agtccttagt actgatgcag
    21001 cagcaactct ttttgttaat gttgaatttt caagtgctaa tgcagtagat ttattttttt
    21061 ctactgtaat catatcattc aacgactcat tttgtgaagc taatgcaaca ttggcatttc
    21121 gcatggagtc taactgtgca acatatacct gattcattga tctcagtcgc tctaactctg
    21181 cttttgcagc ctttaactgt gaagcatcac ccgactcaat catccttctg atttgcgcca
    21241 ttttgctctt aatctcttca tccttactaa gcaactgagc ctgcaagctg gagttctcct
    21301 gatttatttt ttcaaattcg gcttttacct gttgcaactc tgctgacaag gcatcgcgtt
    21361 cagcagaagt attgtcaaga gttcttgtaa cttcttccaa atgacttttt ttatcaaaaa
    21421 actgatatag caacaatccg ttaaccccca atagtatggt gattacaata aatacgagtg
    21481 ctctgcgtga gttttgctca cggttatctg ctttggtttg ttcattcact gattcttcca
    21541 tctatctgaa atgggttgtt aaacggtgca aaataatgta attagtattt acaagcaagt
    21601 aaaaaggatg aaatattctc tgtttctacg taaaatgaca attttgagtt tccttggcca
    21661 ccgcttaatt tgcttttttt attcggtaaa ccttacattt ttttaaacaa aagcatatat
    21721 ttacttcttg atttaaaacc gccacttgtg ataaatgatt tttttaaatt gttcttcccc
    21781 gacaactgcc cgggctgtgg gcatagtttt ttgagaggtg aaaaatatgt gtgtctgcaa
    21841 tgtttgtacg gacttcataa aacacagtat cataacgacc cggaaaatcc tatagcgaaa
    21901 aatttctggg gcaagacaga ttttgtagct gtcacggcag tttatttttt ccagaaaggt
    21961 caggtgctgc aacgactttt acatcagcta aagtacaatg gatgcaaaga aataggcatc
    22021 atgttaggca gacaaatggc tatggagttg tttcaggctt cagcattcag aaatgctgat
    22081 attattattc ctgttccact tcacccggga aaacttttgc aaagaggctt caatcaaagt
    22141 gaaatgattg ccctgggtat ggctgaatat tgggagaaag aagtgcacac agaaactttg
    22201 aaacgaaaaa agtacacaca gtcgcaaaca cataaagcac gctatgaaag atttgaaaat
    22261 acagaaagtg tatttgagct tgacaatatt aagtcttatg aagacaaaca tgttttattg
    22321 gttgacgatg taatctcaac aggctcaacc ataatttcat gtgcagaggc tttttcaggc
    22381 attagcggat gtaaaatatc tatggcttct gttgctgctc ctcatcgttg aatttctgat
    22441 atttgcacag aagtttttag catgtcagag tccatagtta tagatacaac actaagtaag
    22501 caagaaaagt atgcacagct tttgccgcag gcagaagcat tgattaaagg agagacagat
    22561 gctgttgcca atgctgccaa tttatgtgcc atgctcaagc aggcttttgg ttttttttgg
    22621 gtaggatttt attttgtgaa acagcatgaa ggaaaacaac agttgatatt aggtcctttt
    22681 caggggcctg tggcatgtac acgtattgaa ttgggcaaag gagtttgtgg tacagcatgg
    22741 caacagaaag aaacaattgt agtgcctgat gtaaataatt ttgatggaca cattgcctgc
    22801 agctcactaa gccgttcaga aatagtagtt cctatattga agcatgatgt tgttgctgta
    22861 ttagacgtgg acagcgaaca cctgaatcat tttgatgaaa cagataaata ttatcttgag
    22921 aaaataagct cactttattc agaaacattt tcctatgcta ctgaataaaa taaattcatt
    22981 taatagaact tttcttttgc ctgctgtact tcttttgttt ggtgcatgtg cttctgtcgt
    23041 agctcccaca ggtggtaaaa aagatgaaac gccaccaaaa cttaaagagg taattcctcc
    23101 tgagcaaacc gtaaatttta atagtgaaaa agtggttttt acttttgatg agtacattca
    23161 gttgcttgac aaagacaagc agttagtaat ttccccaatt atcgagcccg caccaacatt
    23221 tgacgttaaa ggtaaaaagc tgacaataac ctttaagaaa aagttgaaag aaaatactac
    23281 ttatacaatt aactttggta atgccgttgc cgatttgcat gagtcaaatc cattagcagg
    23341 attcagatat gttttttcta ccggaactta tgttgataca ctttttgtta aaggaaatgt
    23401 agtgtttgca gagaatctga aaagtgaaaa gggaatcctt gtaatgcttt acacatcaga
    23461 agatgattcg ctgccatata ataatccacc tgcctatttt accaggacag atgaaagtgg
    23521 aaatttttca ataaaaaata ttgcacgcgg cccatataaa atctttgcat taaaggatgc
    23581 agataataat tataaattca atcagtctga tgagtggatt gcctttgata aaactcctat
    23641 aaccggaggc gactcaacac ctataaattt gagaatgttt aaagaagctg agaagaaagt
    23701 ttatgttaaa gaagcaaaat catttaacgc ttttgcattt agtgttttga tgaacagacc
    23761 ggttggagta ttaaatattg aacctcttca aacttctgac aatatcaaac taattagccg
    23821 cagtaaaact aatgatacct tgatggtgtg gttaaaagat tctatcagcg atactttaag
    23881 ttataaacta tcaggtgaaa ataattggac cgatacatta acaatatcaa aaccggcaaa
    23941 agcactcaga ggcaaattaa aacaatcaat tagtttgctt tcaagtacaa atatttatcc
    24001 gggaaatgat gtgttactta aaacagctat tcctgttgca tcagtaaata aagaaaaaat
    24061 tttgttgatg cacgatagca ctacatcaca accttttgaa attgtttttt ctgattctgc
    24121 atcatccaac attacaatta aatctgttac tattgaaaag aataattgga atttaacttt
    24181 actaccggga gctgtaacag atatttatgg taacaaaaat gatactgcca aactaagttt
    24241 tcataccaat gcatttactg attacggaag tgtaaaaatt aaagtagatg gtttatcagg
    24301 atcaaattat atacttcaat tggttgacga aaaagacatg gtcatttcag aaaaatatat
    24361 tggtggcaat ggaagctatt tgtttgaaaa aatgcttcct gcaaaatgca gggttcgtat
    24421 tataaatgat gccgatggaa ataaatcttt tacaacaggt aattatcttt taaaacaatt
    24481 gcctgagctt gtatattact ataaagaaac actaacatta cgagctaact gggatattga
    24541 aataaattgg attattaaag aaataaaaca atgacagaac aacaacgaat tgattcaggg
    24601 aaagcaccgg agcctgtagg actttatcca catgcaagac gtgtaggaaa tttattgttt
    24661 ttatcgggtg tgggtccgcg agaaaagggt agcaaaaaaa ttccgggagt agagcaggat
    24721 gcatcaggaa atattgtgca gtatgatata gcagcacaat gtcatgcagt gtttaaaaat
    24781 gtaagataca ttctggaaga ctctggcagc agttgggata agattattga tgtgaccgtt
    24841 tttctgacca acatgaaaga tgattttccg gtttacaata aactatgggc agaatatttt
    24901 aaggagaatc caccatgtcg tacaacagtg gagataaatt gtctgccaac accaatagct
    24961 attgagttga aagttatagc tactatttga ttatgtatct tattacaggc gcttcaggct
    25021 tggttggttc acaacttgtc tttgatttaa tttcaatggg cagacctgtt agaatattgc
    25081 ttcgaaaatc atcttccaca gcgttaatca aaaaaaagtt caagtcatcg cccgagcttt
    25141 ttgataaaat taagattact tacggtgatg tgttggatat tttcagtatt gaagatgcac
    25201 tgcaggattg cacacatgtg tttcactgtg cagcaatggt gtcatttaat cctcaaaagc
    25261 ggaaagagat gtataaagtc aacactgaag gaacagccaa tgtggttaat gcctgtttgg
    25321 aaaaaggaaa tataaaattg ggatttgtaa gttccgtagc aaccttaggt cgtggcacac
    25381 aacagatgat caatgaaaac gactggttca atcagagtga aaaaaacagc tattatgcta
    25441 taagtaaata tgctgcagaa agagaagtgt ggcgtggcat aactgaagga ttagatgcag
    25501 tcattattaa tccgtctgtt atattgggtg atggtaactg gcggtcagat tcatcatcat
    25561 tgtttaatac cttgtacagt ggattgaagt tttatactca aggtgtttca ggttttgttg
    25621 atgtgaaaga cgttagccgc agtctgataa gtttgacaga aagcaaaata tcaggtgagc
    25681 gttttataat atcatcagaa aataaaactt acgagtggtt gtttaagaca atggcaaagg
    25741 ggttaaatat aaatccacca accataaaag caggcatgtt tctttcacaa ttagcctggc
    25801 ggttagaatg gcttaaatct gtttttacag gaaaaccgtc tttaatcacc aatgaaacgg
    25861 catatacatc acagcagatt tacaattacg ataacagtaa aataactgaa caactcaatt
    25921 atcgttttat tcccattgag gaaacagtag aacgggtttg taaattattt atcgcacaaa
    25981 agcaataaaa tccataggct caaaggagtt tactccggcc tgacgaagca tggtgaccaa
    26041 ctgaccacga tgataggtac tgtgattaaa acaatgcatg ataatttctt ccaacggtgt
    26101 ttgatggtct atacccttag tatttttata ggaaatgctt tttttgaaat cctgatcgtt
    26161 cagattagaa atatagttaa tccattcctg cgaactttca ataagcagtt ttgaaaactc
    26221 atttaaatca cctttaaaat ctttactcgg tggccaggaa taaggtttat tttgaatacg
    26281 cataatccag gtgtgttgcg aatcgtatat gtgatacaca gtttttctta tggtattgaa
    26341 actgcttttc atttctatat ctgcagcaac ttcccctgca cttactatgg cattggtaat
    26401 tctttggtta gcccatttat tataattggc atatcgaaaa aatagatttc cactcatgat
    26461 ttttaaaata aatttgaatc aaatataata ttaacagaaa tagtattaat tttattccct
    26521 aagattatca gatatgatta tcaaaccacc atcatcgcaa caactatcag aatattatcg
    26581 tggctattta aaatatcttc cggaagaaga catgctgcaa acacttagaa tagctactga
    26641 tgaaatggaa agtatgctgg aaaaaattcc tgcaaacaaa gagaccttta gatacgcacc
    26701 gggtaagtgg atgcttaaag aagtgattgg gcacttgtgt gatacggaac gaattttaac
    26761 ctaccgtgca ttatgttttg caagaaacga caaaacttca tttcctggtt ttgatgaaaa
    26821 cgaatatgtg gcaaacagca attttaaaaa caggactatg caaagtatag gtattgagta
    26881 cctgattgtg cgcgaagctt cattagcatt atttaattcg cttgatgaaa ctgattttga
    26941 tagaatagga atggcaaatc aaacaaatac ttcagccaga cacatgctgt atttctgcat
    27001 tgttcatcaa cgccatcatt ttaatattat ccgcgaaagg tattacaaat aagctaagat
    27061 tacacttgtt tacggagtac aatacggaaa gtagttcctt tatttggttc agagcttttt
    27121 acaaaaatgt gtccgttatg atactcttca acaattcttt tactcaacga aagccctaat
    27181 ccccaaccac gggttttgga tgtgtaacca ggtttaaaaa cggttttgaa ttttgatttg
    27241 ggaatacctt tgccactatc ggtaatgtca atataaacaa actgcagttg atcggtaatc
    27301 ataacatcaa tttttccttc gccatccatg gcatcaacag catttttgaa aagattttca
    27361 agtacccatt caaataatgg aatgtttaat ggagcaagca cctgatgtgg ttgtgctgtg
    27421 ttcagactaa aaattacctt actcgatgtg cgtgaacgta tgtaggaaat agcgttttct
    27481 ataaccatgt aaacatcttc tttacggagt accggtgcag atccgatttt tgaaaaacgc
    27541 tcggttatgg tattcaatct tgcaacatct ttttcaagtt cagccacatg attctgatcc
    27601 tgtgacgttg acttaaaata ttctatccaa gccatgaggc tgcttaatgg tgttcccagc
    27661 tgatgtgcag tttcttttgc catgcccacc caaacctgat tttgttcggc tttgcgcgaa
    27721 gtgctaaaag caagataact caccaataaa aatagggcaa taatggccaa ttgaaaataa
    27781 gggtagtagc gtaattgata taataaaccg gaatcttcgt aataaatata gtttttctgg
    27841 ttgttggcaa aagagatttc aatcggctca tgtcgctttt tcatttgcaa caacatttcc
    27901 tgaacatatt gagagtcatt gacttttgca gtatcgagat tgatgtgcga taaaatattt
    27961 ccactttcat cagttaaaat agccggcaca gtattgttat cctgcaaaac catgagagaa
    28021 aaactgatgt cgcctgcttc gacagagata ttggcaagtt gtttggatgc ttcggcccaa
    28081 agttgaactt ttttacgttc ttcttcgctt aactttttta ctaaattatt ggtgtaccac
    28141 aacgacaata gccctatgag tatggctgag gccaaaagca aaagcttcca ttgttgtttt
    28201 tgtgtgtaaa tattcacccc tttaaaataa atcgtactta cagttcaggt aaaaatctat
    28261 ctatattcgg atgaattaat tggattacag gtgcaataat acttcttttc tttcaaaaac
    28321 acagaaattc tttttacaaa aattaaaacc gatgcaaccc ttacccctac ttttgcatct
    28381 ttaaagataa tagcagtaat gctcaatgaa caaatcatac aaggttgtaa agacaaacaa
    28441 ggcaaagcac aacatcagct ttacgaactt gtggcaggac gaatgatggg agtttgcctt
    28501 cgttatgcca aggatgatga agaggccaaa gacttgttgc aggaagggtt cattaaagtg
    28561 tttcagcaga ttgatgcctt taggggcgag ggttcttttg aaggttggat taaaaaaatc
    28621 tttatcaaca cagcgttaga gagcataagg aaaagaaaat tgcttacgca accgctggaa
    28681 gatgaagaag atgcaagtga caattttctg attcaggaaa ataaattaga agccaaggaa
    28741 ttgcttcaga tgatcagaaa gttgcccgat ggttacagag tggtttttaa cctctatgct
    28801 attgaagggt ttaatcacag agaaattgca cagcagttga atataacaga aggaacctca
    28861 aagtcgcagt atgctcgggc aaggcagttt ttgcagaaga tgcttacaga agaaaaagta
    28921 aaataattat ccgcaaagga ttgaaaatga aagaagataa aaacatagaa cagatattta
    28981 aagatgcgtt cagcaacttt gaagcacctg ttgatgcttc tttatggcaa ggaatagaaa
    29041 attcactgcc tgcaaatcct gctcctttaa caaaggtgtc attcttagca aaacaggcag
    29101 catatctgaa aattacaagt gcagtcattg ctgttgctac tgccgttaca gcggtgttat
    29161 atttttcaaa taagaatgaa aaagatactg cgcaaaacgt agctcagatt aataaagaaa
    29221 ccactaaagc agttgaacct gctacacagc aaggtgtggt gttaaacaac actgaaacag
    29281 cttccaagtc agaaaaaatc aattcttctg aagagccaaa gactaaaaag tctgcaacag
    29341 ttgagcctgt taagactgag ctgtcagata aagttgttga aaataaatct actcagataa
    29401 attctattcc ggctactgaa acagaagcta ttttaccacc accacagaca gataaacaaa
    29461 ttatatcagg ccccaaaacc aatacaactg ctgagccaat aaaagacact gaggttaaga
    29521 aaacagaaat gaagaagccc gaagccaaaa catcagtcag tgcagaaagt aaaatattac
    29581 ctttgcccgg ccttggcaat atggttttta caccaaatgg cgatggtata ggagattggt
    29641 ttgagtttga tgacagcaat ctgagtgact tgtctgttaa gatttattcc ctgaaaggat
    29701 ataaattggt gagtgagtgg cacacacatg gtggccgatg ggatggcaat cttcaaaacg
    29761 gaaatcctgc cgaagaagga atttatttag tgatgattca ggcaaaaggt atggatcaga
    29821 ctaattacaa aagcacaata aaactgcaat taagaagaaa ttaatattaa caaagtataa
    29881 aaactaaaca tcaaattatc atgagtaaaa ttttagccag attaacagca ttgatgctgg
    29941 tagcaacaat gcttgtgcct gtcagcagta aggcatctca ccttgctgca ggggatattt
    30001 attataccta cacaggtacc ccgaatacgt ttttgataac actacgtctg taccgcgatt
    30061 gcgcaggtat aaccatgtca acatcagaaa cagtatgtta tacttctgca agctgtaata
    30121 tcagccagag tattacagta aacttagttc caggttcagg acagcaaatt ccaccatcac
    30181 catgtgtgcc ttcagcaggc ccaacaacct gccagggtgg tacagcttat ggtattgaag
    30241 aatatctgta tcaagctgtt ttggtaatgc cggcacaatg tattgactgg aaattccagt
    30301 atgaaacatg ttgtcgtaat ggtaacatta caacacttaa caatgctgcg ggtatgggtt
    30361 tctttcttga aacaaccatg aataacctta attacccaac taacagttca cctcacttta
    30421 atacaattcc agttacacag ttttgtgtaa acaatcagtt ttatttcgat cagggggcaa
    30481 cagatcctga taacgattct attacttacg tacttatcaa tgcacaagat gctagtggat
    30541 tttgtccata tactgctttt gacttacagt ataatgcacc ctattcagga gtatatccta
    30601 tttcatcagc taatggtgta actatgaatt tattgacagg cacaattgca tttttaccaa
    30661 acttattaca gaatggtgta attgcagttc gttgttttga atatgatcgt ataacagggt
    30721 tattaaaatc aatcggtaaa cgcgaaattc agattaacat cgtttcaact tgtacagtgg
    30781 ttactccagg cttcgacagt gcacaggttg ccagtggagt aaacattgtt attgatggta
    30841 taaataacgt tacatgtgat gacacaacat tctatattca actaaatcct cctgtacagt
    30901 gtggttctat tgttccgtca gatattcgtt tccttgaccc aagcggactg ccaaatcctg
    30961 ttacttcagc agtaggtgta aactgtgtta atggtcttac agattcaata atggtaacag
    31021 tgcttaatcc aattccaaca ggtacttctt acatatttac taaaattggt aatgatggaa
    31081 acaccttttt aagtgaatgt ggctcgccaa tgaatgagtt tgatacctta acaattaacc
    31141 tttatgactc tgtaccttat acctttgatg tgacagatac attagactgt actttcgact
    31201 cattatttat tgtgagcaat cagctgattg actgcttcac tatcagtgca gatggttcgg
    31261 actttacctt ggttgatgca aacggtactc ctattacaat tacgggagca acttgcttgg
    31321 gtactaacgg tggtcacttt ggaactacat tcctgattac aactgtacct actacaaatg
    31381 cagcaggtcc gttaaccttg atagtcaata caggtagtga cttaaacact attgccaatg
    31441 gttgcggaac aatggtattg caagatgata cacttggaaa tattcaggtt cgtaataata
    31501 ttacattgag tttaggtcct gatgttactg tttgtgccag cgatcctgct cctgttttag
    31561 atgcaggaaa tcacccgggt gcaaccttcg actggttgct gaacggcact tctacaggac
    31621 aaacatcaca aaccataaca ccaacagcaa gtggaactta tatcgttaac gtacagtcat
    31681 cagcttcatg ttttggtagt gatacagttg ttgtaaatct tattccatcg ccttctgtaa
    31741 atttgggtac agatgttaca ctttgtgcaa atgatccatt ccctacatta gatgcaggta
    31801 atcccggtgc tacctatcaa tggtatttaa atgatacatt aatagcaggt gcaacttcac
    31861 aacaattcac gccaacaact gccggtgttt actttgttga agtgagcaca ggagcaactt
    31921 gcttaggacg tgatacaatt aatatttcac aagtacttca attaaatgtt tctgttaacg
    31981 atctttctta ttgcagcggc aatgctgtca cacttcaatc aacatcagca aacagcggtg
    32041 taaactatgc atggacattg aatggtaatc ctatttcagg tcaaacaggc acttcagttc
    32101 cggttaccgg atcaggcgtt tacgcagtaa ctatttcaac aggatcttgt tcagcaattg
    32161 attcagcaat tgtaactgaa gttgaacagc ctacggcttc gttatctaat atttcacagt
    32221 gtgataatgt gacacttcct gttctgaacc ctggttatgt accttctgca ggtacgaact
    32281 ccgttttcag ttggacatta aacagtaatc ctgttggtgg taatgatcag acattaaaca
    32341 ctgctacaac cggtgcaggt aattataatg ttaccattac aaatacatca aacggattta
    32401 cttgtactgc tactgcttca atgacattaa ctatcaatcc tacacctgct gttgagattg
    32461 gaaataatgt tacacagtgt gccggtgcta ctccagcaac tcttgcaaca gcaaacggaa
    32521 caggtaacac ctaccaatgg tcacttaaca ataacatcat agtaggtgct aacgatacaa
    32581 gcattacagc aacttctgca ggtacttata gtgttttagt taccagtgca ggcggatgta
    32641 ccaatacaga taatgtagtg gtttctatca atgcattacc aactcctgaa attcatgacg
    32701 aattgcagaa gttagatagc gtacttttct gtgcaactca aaacccggct ccggtgttaa
    32761 gcttaacaag caacgtgttt attcaaagct acaactggac attggatggt aacaatttat
    32821 ctactaatgc taatgttaca gttgccgcta tcggtgaata tcaggtaacg gtaattgatt
    32881 ctaatggctg tagcaacaca gatgttatcc gagtaattga agcaccatgc gaccttgaaa
    32941 tatttaacgt aattacgcca aatggtgatg gtaagaacga tgtgtttgag gttaagaatc
    33001 taatggactt caagactcgt aaactcaccg ttttcaaccg ttggggtaag aaagtttata
    33061 gtgccgaccc atataacaac gattgggatg gtggcaatca ggctgcagga gtttattact
    33121 tcgttcttga gtatgacaat ggtatcaaat ccggtgacaa aaaaggatcg tttaatatta
    33181 ttcgataagt tgccgatagt ttataatttt gcaggagcgt tcttaaaaag aacgctcctg
    33241 ttttttttaa tactgttgca aatattttat gaaagtaacc gaacatttta aaaattctaa
    33301 aaagacttta ttcagcattg aaatactgcc tccgctgaaa ggaaaaagta ttcaatctat
    33361 ttttaataca atagatccgt tgatggagtt taaacctccg tttattgatg ttacttatca
    33421 tcgtgaagag tttgtttata aaaagcgtga aggcggttat ctcgaaaaaa tttcaataag
    33481 aaaacgtccc ggcactgttg gcatttgcgc agccatcatg aatcattata aagttgatgc
    33541 tgttcctcat attatctgcg gaggattttc acgcgaagaa acagagaatg cattaattga
    33601 ccttaacttt cttggaatag acaatgtgct tttactaaga ggtgattcag aaaaacatga
    33661 atcgcatttt acacctgaac ccggtggaca tgcctatgct cttgaattgg ttgaacaggt
    33721 agtaaatatg aatgtgggta catttttaga cgatgatctt aaaggtgtag agccaacaaa
    33781 attttgtatt ggtgtagccg gttatcccga aaaacatttt gaagccccta atctgaactc
    33841 agacatttct ttccttaaga aaaaagtaga tgccggtgca cagtatatcg ttacacaaat
    33901 gttttacgac aatcagaaat atttcaactt tgtagataaa tgccgtaaag caggaattaa
    33961 tgtgccaata attcccggac taaaaccaat ttcaaataaa aaacaaactg ccatactacc
    34021 aaaaactttt tttattgata tacctgatga cctgattcag gaaattgaaa aatgcaagag
    34081 tgatgatgca gtaaaacaag caggtattga ttgggcggtg acacaagcta aagaacttat
    34141 taaatttggt gtgccctgtc ttcattttta tactatgggc aatccggaga atatcagaaa
    34201 aattgcatca caattgtttt aatgtttcaa gcatccatac ataaggctga aacttttaaa
    34261 cacgacaggt gtttatatca caaactccac tcagttgaac cggttttcga ttaaagacat
    34321 agaaattctt acaggaatta agccgcatac cattcgaata tgggagcagc gttatggtat
    34381 tattaaacca cagcgaactg caaccaatat ccgttattac acagaggaag atttgaagtt
    34441 gatgctcaat gtttctttgt tgaataagat gggatttaag atttcgaaaa taacttgtct
    34501 taaaccgaat gaaatcgaac ataaagtttt gtcgttagta gaacaggcca atgatgctga
    34561 tataaacctc agtgcattgt tgaattgtat ggtcaggctt gatgaggata cttttgaaca
    34621 cctgctaaat aaatatctgg agacaaatgg gttggaaagt acaatgacaa gaattctgtt
    34681 ccccttttta tacagaatag gattgctttg gcagacaggc tccattacac cggcctacga
    34741 acattttttt accaatcttg tacgtcagaa aataattgca gcaatagaca agcaggtatt
    34801 gtcgccactg cctgatgcaa aaagattttt actctttctg ccacctaacg aaccgcatga
    34861 attagggttg ttgtatgcac actatgtaat cagacaacgt ggtcacagat cagtttatat
    34921 gggtcttaac ttaccgatag atcatatcat ttgtgctcac gaatgttgtc agcccgattt
    34981 tattgtgact acattaacat catgcataaa gattaacgct attgaagaat ttattgtaca
    35041 gatttcaaaa acattttcaa aaagtaaaat attactttcc ggtgcacagg cagtaaaccg
    35101 aagtttgcat ctgccacaaa atgttcaggt aatctctgac tttgatgact ttattcaata
    35161 cattgaaaac agagtcaatt aatgcatact ttattttatt cattactgta aaaattgtag
    35221 ctttgtttta aatcctgata gaaatatgtt tattcaacaa ttatatacaa gttgcctagc
    35281 cgaagcggcc tattatatag agtctgatgg gcaggctgca attatagatc ccattcgtga
    35341 aacaacacct tatttgtcat tgtcggaaca acgccatgca cgcataacac atatttttga
    35401 aacacatttt catgccgact ttatcagtgg acacattgat ttagcaaata aaactggcgc
    35461 aaaaattgtt tatggccccg atgcgcatac caactataca gtacataatg caaaggatgg
    35521 agaagagttt actattggta agcttactat taaagcaatt catactcccg gacatacctt
    35581 agagtcaacc tgctatttat tgcttgatga aaacaaaaat cctcattgta tttttacagg
    35641 tgacactttg tttgttggtg atgtaggcag acccgacctg cttgatggca gtggtatttc
    35701 aaaagagttt ttagcagata aaatgtttga ctctttaaat tcaaaaataa aaacattgcc
    35761 tgatgatgtt attgtttatc cggctcatgg cccgggctct gcatgtggaa aaaatatagg
    35821 aaaagaaaca tggagtacta ttggtcagca aaaacaatca aactattatt tgaaagaaaa
    35881 agacaaagca tcttttataa aaaatctgac aacaggaatt cttccaccac ccatgtattt
    35941 ttttgaagat gcaagaatca acaaacaagg atacaggccc attgaagaag taattgtcag
    36001 aaatgcaaag ccgttggcat tagaagattt taagaatgaa gtaaataaag gaacactgat
    36061 tctcgataca agaaatgctg atgattttga aaaaggtttt gttcccggct ctgtaaatat
    36121 cggactcaat ggacagtatg ctaattgggt gggaacacta attccaattc aacaaccaat
    36181 agtactggta acagaaaacg gaaaggaaga agaaagtgta cttcgtgctg cgcgcgtggg
    36241 ttacgaaaac ataacaggtt atcttgaagg aggaatagag agctggaaaa acaacggact
    36301 ggagtttcat actatcagct ctgttgatgc agccacattt accaatttac taaaaacagg
    36361 atcatatact gctttggatg taagaaagcc cggtgagtat gaatcatcac atgtaaaaga
    36421 ttctgtcttt gtttcgttga gcgacattcc acaacactac aataagttgg ataaatcaat
    36481 aacgtatctg atattttgtg gtggaggcta tcgcagtatg atagcagcat cgttactaaa
    36541 aaagaatgga tttgaaaatg ttgttaatgt gtatggcggc tttggaaaaa ttaaagattg
    36601 cagcatatca ttagaaactt cacaacctgt gatgtgaaca cagtccatga aataacaaca
    36661 tccaatacca atgtgctgat agatttttat gccgattggt gcgaaccctg taagtggctc
    36721 aatccggttt tggaggagtt gacgcaacaa cttcccgatt tgtatatcca taaaatcaat
    36781 attgaccaat ttactgaaga ggctgcagca tttgaaataa agagtgtgcc tgtgctgata
    36841 ctttttgttg atgggaaaca ggtttggcgt atgaacgggt ttaagtatgc gaaagaatta
    36901 gccaaagagt taaaggagtt cttaaaagcc tgagacctta ctttatagct gttgaaaaca
    36961 tttatttttt aatgtgtagc atgactaaca tctgtttcac tacacgtaat tttacaatca
    37021 aacgaaaaca tgtcaaagaa gaatataaaa ttcacaaaaa ca
//
LOCUS       DAOOOL010000008        36259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_82_length_36259_cov_107.217876, whole genome shotgun sequence.
ACCESSION   DAOOOL010000008 DAOOOL010000000
VERSION     DAOOOL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 36259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36259
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82_length_36259_cov_107.217876"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="PKU82_01435"
     CDS             <1..133
                     /locus_tag="PKU82_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOZ81564.1"
                     /translation="HAFTNPEATAVGKKFDMPISYNAEADKKSWLDMKAFLAEHLKK"
     gene            complement(135..923)
                     /locus_tag="PKU82_01440"
     CDS             complement(135..923)
                     /locus_tag="PKU82_01440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ81565.1"
                     /translation="MDYLDLNKKLWNERTNVHVDSAFYDNETFLKGKNSLNEIELNLL
                     GDVKGKTILHLQCHFGQDSISLARLGAQVTAVDFSEKAIETAIELNKKSGTGCKFVCS
                     DVYSLNTVLDQKFDIVFTSYGVIGWLPDMQKWADIISSFLKPGGVFVMAEFHPAVWMF
                     DNDFKYVQYSYFKSAPIIETDEGTYADKQAKITTTSVTWNHSLSEVMSSLLNSGLRIE
                     SFNEFDYSPYNCLNNMVEVSKNHFMIKGMENKLPLVYALSASKQ"
     gene            1009..2067
                     /locus_tag="PKU82_01445"
     CDS             1009..2067
                     /locus_tag="PKU82_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ81566.1"
                     /translation="MSHILLIEDNKEVRENTAEILELAGYTVTTASDGKKGIEAVQKN
                     KPALIICDIMMPVLDGYGVLHLLGKNPETSTIPFIFLTAKTERGDFRKGMEMGADDYI
                     TKPFDKLELLNAIESRLKKVEALKKEYSKDLEGLTNFIGEVGGKDHMQAFIENRKIRT
                     YKKKENIYKEGDYPRGMYYINHGKVKLYKSHAYGKELITNLLKEGDFFGYTSLIEEGD
                     YVESAQTLEDTEVVFIPKEDFLNLLFSNREIAKRFLLILNQKFNEQQDYLIELAYSSV
                     RKRVAEALILLSDKYKANRDERFSMSISREDLANIVGTATESLIRTLSDFKEEKLIEV
                     KDGKIVLINENKLKELKN"
     gene            complement(2103..2807)
                     /locus_tag="PKU82_01450"
     CDS             complement(2103..2807)
                     /locus_tag="PKU82_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOZ81567.1"
                     /translation="MFKSLLTIALGLSLSFAGAQTSKQDTVLTPDPDKAGLFDCRINF
                     PDGRARAVGKIQNGKKEGLWRFYDTKGKIELLEEFEADVLNGLHLHFNENGYIEDEES
                     YRKGILHGLRNVYKYGVTLITSENYNNGKLDGMRFDYYENGKLKEKATYKNGIRDGIT
                     IWFKQDDKPTIQYTYKNGDLNGPAIFYANGEIQSEGNYVNNNEEGEWRVYEDGKLLKT
                     ILYKNGKVIKETAAKK"
     gene            2916..3989
                     /gene="prfA"
                     /locus_tag="PKU82_01455"
     CDS             2916..3989
                     /gene="prfA"
                     /locus_tag="PKU82_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOZ81568.1"
                     /translation="MLEKLEALKHRFEEIGQQLSDPSATTDMRRFQQLSRQYKELQEV
                     VETYERYKKVIDNYDQAYHVLNTEKDVDLKQMAKEEMETLEAEKNSLEDQIRLLLIPK
                     DPIDERNAVVEIRAGTGGDEASIFAGDLYRMYSRYFEKKGWKVDVIDENEGTAGGFKE
                     VIMEVSGDDVYGIMKYESGVHRVQRVPDTETMGRVHTSAATVVVLPEADDVDVVINPA
                     DLEFQTARSSGAGGQNVNKVETKVQLTHKPTGIMVTCQIERSQHGNRERALHMLKTKL
                     YEIELQKHESEIAARRKTMVSTGDRSAKIRTYNYPQSRVTDHRIGLSTYNLSSFMNGD
                     IQEFIDALQLAENAEKMKEASLS"
     gene            3993..4814
                     /gene="pyrF"
                     /locus_tag="PKU82_01460"
     CDS             3993..4814
                     /gene="pyrF"
                     /locus_tag="PKU82_01460"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOZ81569.1"
                     /translation="MNYQQLVGEIKRKQSFLCVGLDTDLKKIPSHIRDKADDPVFEFN
                     KAIIDATLDYAIAYKPNLAFYEASGVEGLKSLEKTIQYINGRAFTIADAKRGDIGNTA
                     EMYAHAFYNHYGFDSITLSPYMGADTIEPFLKHKDKWSIILALTSNPGAEDFEMLKVD
                     DDFLFEKVLKKFSKASAQQIMFVVGATRPQYFEIVRKHVPDNFLLVPGIGAQGGSLED
                     VCRYGLNSNAGLIVNSSRQIIYADSGIDFDKKAAEQAKQIQQQMKQMLVEFKITG"
     gene            4814..5548
                     /locus_tag="PKU82_01465"
     CDS             4814..5548
                     /locus_tag="PKU82_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HOZ81570.1"
                     /translation="MPFTLAHPAIVIPFYRLFKERLSLTGLIIGSMVPDVEYCVNTVT
                     RSVISHTEKGIWVFDLPVAIFLTFCYHGFVKQILTNNLPDWIRYRLSPYANKNWFHYF
                     RKNFLNYFLCLLAGIVLHLLWDSVSHGTGYFVRRTAFLQENVIGTLTVTRLIWHISTV
                     AGLYVVIRFFLSFPVNKMEEENNIPDKNFWPLVFLVASVLTIIHWLPFVRPQEPRFIL
                     VAFSGAMMVGVIVVCFIFRFFKKQLS"
     gene            5545..6819
                     /locus_tag="PKU82_01470"
     CDS             5545..6819
                     /locus_tag="PKU82_01470"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOZ81571.1"
                     /translation="MNEALLYHLWKYRLFKSLPLTTIDGKTIEVIKPGELNPDSGPDF
                     FNARLIIDGTEWAGNVEIDPTSADWYNHKHHINKAYSKLILHVVYEMNKPLDHGFPVL
                     EFKKFVDDGAVKTFHQLQESLLPIPCSKQVHHVNDVTLNTWLERMIIERLEQKTTVIH
                     DMLHANKFDWEETFYFFLARNFGFKTNALPFEMLARSIPMNALARHKNNLLQIEALLF
                     GQAGFLNDKHADEYIESLRREYRFLAGKFQLQAMNNSLWKFARMHPRNFPSVRIAQFA
                     ALIHNSTHLFSKILEEKDVKSLIQLFSITPSSYWDKHFHFGKESAESKKTFGRSSVDN
                     IIINTIAPFLFLYGKIKDNEKLTERALSLLEHIKPENNVIIRQWKEVGITVKHAYHTQ
                     ALIQLKNLYCSKKKCLSCALGVKILNNKKNDY"
     gene            6815..7033
                     /locus_tag="PKU82_01475"
     CDS             6815..7033
                     /locus_tag="PKU82_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOZ81572.1"
                     /translation="MDSIKNFFERQAFGICEYWGERLKIRSSVIRLFFIYLSFLTIGS
                     PVVVYMVLGFWMNLRKLIHRQRGGVWDL"
     gene            7079..7444
                     /locus_tag="PKU82_01480"
     CDS             7079..7444
                     /locus_tag="PKU82_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOZ81573.1"
                     /translation="MYIKPTCSTCRTAMQMAKECETEIEMIEYLIDTPSEKELLELTQ
                     QLGVKPFDLVRTKESLYKEKYEGKKISDKQWLKILHKNPILIERPIITDGTHAIIGRP
                     PTLIADFLNKKQKTKKKSK"
     gene            7517..9361
                     /locus_tag="PKU82_01485"
     CDS             7517..9361
                     /locus_tag="PKU82_01485"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ81574.1"
                     /translation="MLSAIQVNAQHDDLFIPRNIQKAYQNNTRTIEGAPGKKFWKNFA
                     EYFIQVKFNPKTLKIEGKQIITYYNNSPDTLKKLVFRIAPDFYKKGNERNRIIEPDDV
                     TDGVKFTNCVIDANKVDLNNPMLVKHEGTIMEIKVKGIDPRGGRATVEMDWSYTLNLN
                     SHERTGVIDIGSYFIAYFFPRITVYDDVDGWDEFPYLGQQETYFDNASFNVHITVPRN
                     FMVWATGDLQNPAEVLHESVLKKWTLAQQSNKPVFLIDSADIQNKTVTVNKKFNTFQF
                     KCTTPDFVFAVSNHYLWQARDMVVDSVTMRKALISTAFNPIHKDFFEVNDFTAKTIEV
                     MSFDFPGVAYPYSHLTVVDGRDQMEYPMMINDNPTTTRFDGITLTTHEVFHQIFPFMM
                     ATNQTKYAFMDEGWATIGEWYISPIIDSTIVDDYGMDRINSDLGKDWDTPMITNSTEL
                     TKSLFINSYPKPALTFLYLWEMMGDSLFKKSLQHYIITWMDKSPTPWDFFFSMNQSCG
                     MNLNWFWKAWYFEQGYPDLGIIGINRLNYSYTVTIKSIGNKPVPVHLTATFADGTKRK
                     FTRTVDIWKNRNITDVIIDTQQSITKLELGDVYDADVDKNNNVLVPKE"
     gene            9394..9465
                     /locus_tag="PKU82_01490"
     tRNA            9394..9465
                     /locus_tag="PKU82_01490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9427..9429,aa:Arg,seq:cct)
     gene            9439..9561
                     /locus_tag="PKU82_01495"
     CDS             9439..9561
                     /locus_tag="PKU82_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81575.1"
                     /translation="MAVRFCPGLRRKGSSLSDYEVGGDSARDYEEKAVPCPATK"
     gene            9601..9900
                     /locus_tag="PKU82_01500"
     CDS             9601..9900
                     /locus_tag="PKU82_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOZ81576.1"
                     /translation="MPYYVYVIRNKVTGRLYKGQTENLEKRLKAHSQSQTKTTSYFPK
                     DWELVYHEIFSSRKEAVEREKYFKTAAGRRYLSGRIANIKSTDTRPNVPFGTGGE"
     gene            10188..10601
                     /locus_tag="PKU82_01505"
     CDS             10188..10601
                     /locus_tag="PKU82_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOZ81577.1"
                     /translation="MHIHLFREQLKQKGLKVTPQRVAIYEAVAELHNHPTAENIIEYI
                     KTNHPNISVGTVYKVLDSLVENNLLRKVKNEKDIMRYDAVLEHHHHLYCAESDRIEDF
                     EDPQLDHFISEYFKKKKIKNFKVQDIKLQITGTFK"
     gene            10622..12805
                     /gene="katG"
                     /locus_tag="PKU82_01510"
     CDS             10622..12805
                     /gene="katG"
                     /locus_tag="PKU82_01510"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOZ81578.1"
                     /translation="MDNTKSGKCPFHHGANTEASTGVMNWWPKALNLDILHQHDTKTN
                     PNGEKFNYAEEFKKLDLEALKSDLKKLMTESQEWWPADHGSYSGMFARTAWHLAGTYR
                     KADGRGGANTGNQRFAPLNSWPDNVNTDKGRRLLWPIKKKYGNKISWGDLIVLAGNMA
                     YEVAGLKTFGFGGGREDIWHPEKDIYWGSEKQWLAPTGSEGTRYSGERDLENPLAAVM
                     MGLIYVNPEGVDGKPDPLKTAQDIRVTFSRMAMSDEETVALTAGGHTVGKAHGNGKVE
                     VLGGVPEAAEIQHQGLGWFNPDGSGNKDKTMASGIEGAWTTDPTKWDNGFFDLLLNYD
                     WALTKSPAGAWQYEPTNMPEEKKPVDAFDPKVRRNPMMTDADMALKMDPEFRKISERF
                     YNDFNYFSETFARAWFKLTHRDMGPKSRYLGADVPKEDLIWQDPIPAVSYTLSDAEAD
                     EIKTKILNSGLSRTELINTAWDSARTYRGSDFRGGANGARIRLEPMKNWEGNEPKRLQ
                     KVLDKLTEIQKGLSKKVSIADLIVLGGSAAVEQAAKDAGMSVKVPFAAGRGDATLEQT
                     DVDSFSDLEPLHDGYRNYQQKNYAVSSEELLLDRTQLMGLTAPEMVVLVGGMRVLGTN
                     FGGSKHGVFTEREGVLTNDFFVNLTDMKYEWKPVSENLFNIVDRKTGATKYTATRVDL
                     VFGSNSILRSYSEVYAQDDNKEKFVKDFVKAWVKVMNADRYDLKK"
     gene            complement(12887..15457)
                     /locus_tag="PKU82_01515"
     CDS             complement(12887..15457)
                     /locus_tag="PKU82_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOZ81579.1"
                     /translation="MIPFFGVAQITDNFSDGDFTANPAWSGDNSQFIVNASQQLQLNS
                     TGAATSYLALTTSLPTLDNTSWQFYIKLNFAPSSSNYARIYLVSDQQNLKGALNGYYV
                     QFGETGSNDAIEIFKQTGTTSVSVARGTNGFIAAASTISVKITRDNAGLWSIYADAAA
                     GTNFTLQSSGTDATINSGSYFGVSCTYTSSNATGFFFDDFSIPFIADITPPTLTSAIP
                     LSNTALDVKFSELVDLTTSQTTTNYAIQGLGTPATAVRDISDFSLVHLTFSTPFTSGT
                     AYTLSVSNVSDLNSNVIAAGSTIGFTWNAPVTAQPFDVVINEIYFEPIVSAPLPNAEF
                     VELYNRSNKILSLNGWTLSDGSTSVASIGAVTLQPSAYIIVCKNSDTTSFIPYGNTTG
                     TSSFPSLNNDVGDDLKLTDNNGTLIDRVIFSDDNYNDDSKKTGGFTIERIDADFTCNS
                     RFNWHASNDAQGGTPGKVNSVKGTYSDTTPPQAVDAYPLTTTLIRITFSENPDNAIAS
                     QTSSYIIDNGIGQPASVLLSDNHAFLTLNNALQTGIVYHVNLNSSIADCPGNKITGEL
                     SLKIGIAETAVAGDVLINEILFNPKTGGNDFVELYNNSSKIIDLKNLKVATTDNNNLI
                     TTNYSVSDGGYLLFPKEYIALTSDVASLQSFYPNAATNNLLKASLPGFNDDMGVCVIT
                     DFALNRIDQLNYNKRWHFPLIDDQNGVSLERIWFNKGTQDSTNWHSAAEAVGYATPGY
                     KNSQSLNEQLQENNFTITPEIFSPDNDGYNDVVTFNINMNEPGYMLNAKIYNEAGQLV
                     RNLVKSKLLAPEDSFMWDGITDKNDKASVGIYIVYAEAFNPEGKTKKYKKAFVVASKL
                     "
     gene            15602..16594
                     /locus_tag="PKU82_01520"
     CDS             15602..16594
                     /locus_tag="PKU82_01520"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOZ81580.1"
                     /translation="MKVAVVGATGLVGKTMMRILEERKFPVSEFIPVATANSAGKKVM
                     FSGKEYTVITCEEAVALKPDIALFSAGGSTSLEWAPRFAQVGTTVIDNSSAWRMNEKC
                     PLVVPEINADVLTKEDKIIANPNCSTIQMVMVLNPLHKKYKLKRIVVSTYQSVSGTGK
                     KAVDQLMNERKGISGAMAYPYNIDMNVLPHVDSFLENGYTKEEMKMVNETRKIMRSDK
                     IKVTATTVRVPVVGGHSEAVNAEFENQFELSDVRQLLSHTAGVTVIDDPAQLQYPMPT
                     YAEGKDDVFVGRIRRDESQPQTLNLWVVSDNLRKGAATNAVQIAEYLLKQFWQH"
     gene            16861..17100
                     /locus_tag="PKU82_01525"
     CDS             16861..17100
                     /locus_tag="PKU82_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOZ81581.1"
                     /translation="MNWQEYIMSDEEVLLGKPTIKGTRISVEHIVGLLAQGWTEKEIL
                     ENFPRLTKESLQAVFLYIQDCFSDGLLFTPKRKTA"
     gene            17110..17463
                     /locus_tag="PKU82_01530"
     CDS             17110..17463
                     /locus_tag="PKU82_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOZ81582.1"
                     /translation="MKLLANENFPLKSIVYLQNGGFDITSIGAEHSSIKDFEVMTIAI
                     NEERTILTFDRDYGELIFKHNYKPPKGVIYLRLEKYSADEPGRLIEELVSSNQYIFDG
                     ALTVLDERGIRQRKY"
     gene            complement(17600..18277)
                     /locus_tag="PKU82_01535"
     CDS             complement(17600..18277)
                     /locus_tag="PKU82_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOZ81583.1"
                     /translation="MGIYLIVIVFMLVGWLVSAQLKRKFEQYSHETLGTGLSGQEIAE
                     KMLHDNGINDVKVISVEGQLTDHYNPLNKTVNLSQPVYYGRNAAAAAVAAHECGHAVQ
                     HAKAYTMLQVRSALVPVVSFASKWVQWVLLGGVLLINVFPSLLLAGIVLFAMTVLFSF
                     ITLPVEFDASRRALVWLSDSRMVTSSEHAKAKDALWWAAMTYVVAALGALATLMYYVL
                     IYFGRRN"
     gene            complement(18401..19492)
                     /locus_tag="PKU82_01540"
     CDS             complement(18401..19492)
                     /locus_tag="PKU82_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ81584.1"
                     /translation="MSYSQTKAMLAIAKASFRSITRSPSAVVFTLVFPLTFILVFGFL
                     SGTGLKVKLFLTPSSDHHNPIYEATKNIDVINWIEGDSAIASKKLQQGKIDGILVITK
                     NITEGKIDYTARLITSTAAPEGGALSKNILDNLISKINLYDAHVAHPLAQLKNEEIAG
                     REYKKIDFILPGQLGFALLSTGVFGTAFVFFSLRQTLVIKRFFATPVKRIYIVLGETL
                     SRMVFSLLGACFIIGVGRFAFGFTLINGISTFLTMMLICAFGLIVFMGFGFVVSGIAK
                     SESTIPPIANIITMPQFLLSGTFFPIDVFPKWLQPFCKALPLTYLNDALREVAFEGAG
                     LIDVISQLMVLLLWGVIIYIVAAKTFRWE"
     gene            19593..20144
                     /locus_tag="PKU82_01545"
     CDS             19593..20144
                     /locus_tag="PKU82_01545"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOZ81585.1"
                     /translation="MNHTFSLFQSMERDKIILSFKGDITQDLLASVYQIMESRMDQDK
                     TDSKRKKRFYHILVECLQNVYHHMEDFRNVHELDEISDGSAIFIIAKAKGEGYRIITG
                     NYIKKDESEDLKSKLEKINSMNTDELRTYYLETLSTTELSQKGGAGLGMIDIARKSGH
                     KIVYSFDGVSDTVNFFTLEVTIN"
     gene            20155..20538
                     /locus_tag="PKU82_01550"
     CDS             20155..20538
                     /locus_tag="PKU82_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOZ81586.1"
                     /translation="MDKLIIEPGIKTPHIVFDAANGSLEIKGKSIPENSIEFYKPVYD
                     WLDTYTTQPKDQTLLTVQLEYFNTSSSKCLLDLFRKLENIHSSGKGKVNVKWLYEEDD
                     EDIMEAGDDYQTIVKLPFSIEKISG"
     gene            complement(20539..22383)
                     /locus_tag="PKU82_01555"
     CDS             complement(20539..22383)
                     /locus_tag="PKU82_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ81587.1"
                     /translation="MKNILFLILIPFTMFAKKPEDERGIYVKDQHSYSNPAEIVVQHL
                     DLSLKADFSTKKLVGFVTLQVKNLTNADTLRLDTYNLKIESVKINDAPVTFILKPADE
                     ILGSELAIPITANTKEVTIYYETSPDAQALQWLNPEQTAGKKHPFLYTQSEAILARSW
                     IPLQDTPGIKFTWTATIHCPKELMALMSCPQNPKQIGNGTYSFKMPFAVPSYLMALSI
                     GDLAYKAYDNRSGIYAEPVMLAKAFHEFTDLPAMISSAEQLYGKYPWLQYDVLVLPPS
                     FPFGGMENPCLTFATPTIITGDRSLVSLIAHELAHSWSGNLVTNATWNDFWLNEGFTV
                     YFESRIMEKIYGKDYADMLNLLSQNELKHTVADMGVNNPDTKLFLDLKGRDPDDGMGD
                     IAYEKGRFFLMALEEAAGRDMWDAFLKKYFEEHRFQTMTTKEFLHYLEVNLLQENPLI
                     KQKVNVDEWVYGTGLPKSLPVIKSKYFDQVEKAAAAFIKQPTVMPDTAGWTTHHYLHF
                     LRTIAPEMTYEKAVALDNHFHFTQIQNSEIAFDWFMIAFACHYKPAYKALENFLNTVG
                     RRKFIAPLYRKAAADATLRPWIEQIYKSARPGYHAVAVKTIDEIFEGK"
     gene            complement(22391..22840)
                     /locus_tag="PKU82_01560"
     CDS             complement(22391..22840)
                     /locus_tag="PKU82_01560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ81588.1"
                     /translation="MNIFWKFKSFNDLTAHELHDFLQLRQQVFIVEQNCPYGDIDKTD
                     KLSFHLMAMANQQVVGYARIIPPGVLYDEVSVGRIVTHQQLRRTGLGKLVVEKSLDEC
                     KSLFPNSAIKIMAQSYLRKFYESFGFVVISEEFLEDNIPHIYMIKTK"
     gene            22980..24008
                     /locus_tag="PKU82_01565"
     CDS             22980..24008
                     /locus_tag="PKU82_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOZ81589.1"
                     /translation="MSLPQLIVTDSSRNGKLPFAPFKRTQEESAQQPKTEKVSNKVAK
                     QKLSLKTDSLKGNTTVVDTIKPIANDSIQLAIDSSVADMSRLFRFTDEENDELNNSDQ
                     QFISSSAYFKQQQLEVKSSSPQLFNKIVPDWFTIVLFFLIVGITTVKVFYQNIFRQLV
                     EAVYSFAVTNQVVRDENLLVQRASVLLNVIFYCSSGLFFYWISVRYNWNHPILNEGLF
                     RFFFFAFVTAVFFTVKMLLLKLIGTIFNLDKPVAVFIFSIFLTNNLLGVLLVLITSFM
                     AYLSGVGLINYIYLVLGILSLAFIFLLYRAFLISRTVQGYSLYYLILYFCTLEIVPIA
                     LIVKFASE"
     gene            24032..24787
                     /locus_tag="PKU82_01570"
     CDS             24032..24787
                     /locus_tag="PKU82_01570"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOZ81590.1"
                     /translation="MAGNSKVKTILVTQPKPETEKNPYFDLAKKFNVKIDFRPFIQIE
                     GIPAKDFRKDRVNVAEFQAVILTSRQAADHYFRMAAEMRMTNFDEVKFFCVSESTAFY
                     LQKYITYRKRKVFYGNNSINDLTDVLKKHKKEKFLLPCSEVMNPDIINTLKDGDLNFI
                     TAAMYRTVSSDLSDVNIETFDMIVFFSPSGVQSLLKNFPKYKQGNTRIATFGVTTSQT
                     AKDAKLKVEVEAPVPQAPSMTMAIEQYLKKANK"
     gene            complement(24792..26012)
                     /locus_tag="PKU82_01575"
     CDS             complement(24792..26012)
                     /locus_tag="PKU82_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81591.1"
                     /translation="MGKGISEFIWRKIIMLSRKIEIFKTSDCVDKERLTKYVQGKMTD
                     AEKYAVEKHLTDCELCSDAAEGLALLTSMKPLDETIKQVSDKYSSSAKQSPSINRWYY
                     AAASVAILIATGWIISNYSFKHENTAEQINAPAVQQKINTETPQTSSDTKLNESTIQP
                     PPPPAEERIQQASKNIIASHNAMVAKASKEDKAFAATEPVQKNEAIAEPQSADVAAPI
                     VTESASAGAAVVDAVVPVTKNIEGLKVYDYSREYKREKNVAKEDNGIPARYESRKDME
                     KSKTLNNMQSRTIGYQDFLAEALVDYNNKKYSSAINKLQLLLSDNHSDVNALFYTAMS
                     YSESQQYDKALHFLDRLDAQSNNTFNQESAWHRALLLLQKGEQDKAKELLQKIISSKG
                     FYATQAQQKLSEIK"
     gene            complement(25957..26553)
                     /locus_tag="PKU82_01580"
     CDS             complement(25957..26553)
                     /locus_tag="PKU82_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ81592.1"
                     /translation="MWLIRNKDKNALSDEELVLQYRQQGDKQAVGILFNRYSHLVFGV
                     CMKYLKDTDDSKDAVLQIFEKLYADLSKHDVQKFSYWIHRVAQNFCLMQLRSRQAAKN
                     RADNYSRDEIAASTEFEPENTDRVYEKETELLMLEEAIKTLNEEQRICIELFFLKEKC
                     YNEITDLTGFDFKQVKSFIQNGKRNLRIYMEKNNHVKS"
     gene            complement(26635..26913)
                     /locus_tag="PKU82_01585"
     CDS             complement(26635..26913)
                     /locus_tag="PKU82_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81593.1"
                     /translation="MELTIIAKEDLSDYHFVQHEVLSNEIDRARRKILLDGAMALGND
                     YKQKVRIVFETTEGTCAVETTVWAVTNDHVELKAGKDIPIHAIAEVVL"
     gene            complement(26979..28010)
                     /locus_tag="PKU82_01590"
     CDS             complement(26979..28010)
                     /locus_tag="PKU82_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81594.1"
                     /translation="MQRIFHILWCFCFTSSLQAQQIELKTIQLPVQVSIRALQTVNDS
                     VVWFAANRGVFGFTKDGGSNWHIDSIATDGKYSEFRSLAALNDSTVLLLSIDSPAYLL
                     RTNNYGTSWQHVYSNHSKGIFFDSMTFKDSLHGIAISDPMEGYFMIIKTTDGGNNWQE
                     LKVTSPAIKGEGCFAASNTNIQWLQNSVIFATGGSTSRLFISENDGAFLPQTTPMISD
                     STMTGIFTMYFENKLNGYIGGGNYLQSDTTTTLYQTNNGGKTWQPIKLPEGAFVSCVK
                     TIKTKNGNYLITTGHNGTYVIDSQHYIKEIKNADGLSLAFYTISIAPSQKKVWMAGKS
                     GKMAVLKFY"
     gene            28179..29399
                     /gene="dinB"
                     /locus_tag="PKU82_01595"
     CDS             28179..29399
                     /gene="dinB"
                     /locus_tag="PKU82_01595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOZ81595.1"
                     /translation="MSAQLNRVIAHMDLDCFFVSVSRLLHPELNNKPVIVGGGERGVV
                     AACSYETRKFGVHSAMPIKQAKRLCPEAIIVRGDYDAYSSHSDMVHEIICESVPLYER
                     SSIDEFYLDLTGMDRFFGCYKHATELRQRIIRETGLPISFGMSANKTVSKIATDEAKP
                     NNQMQVDCGEEKIFLAPLSVRKIPMVGEKTYTLLRSMGVEKVKTVQEMPVELMERVLG
                     ENGTTIWKKANGIDTTAVEPYHEQKSMSTEETFEQDTIDVQRLQHILIAMTEKLCFRL
                     RSEHKVTSCISVKIRYSNFDTHTMQCRIAYTSCDHIIIKQVKELFAKLFNRRMLIRLI
                     GIKFSHLVGGGHQINLFEDSEEIINLYQAMDRMRLRFGEDKIHRAAAMSFSLRGFNPF
                     NGLSNSPKEIKGGK"
     gene            complement(29556..30533)
                     /locus_tag="PKU82_01600"
     CDS             complement(29556..30533)
                     /locus_tag="PKU82_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ81596.1"
                     /translation="MLISVYVPYHAAIEAITPAYRLFKTANDILIASGKKPKFKVEYV
                     GLQKSIKANDGEYSIRIDRHINSVNDSDLIIIPALYGDVKHSLKKNAAAIPWLQKMHR
                     QGTEVASLCIGAFLLTASGLVDGKKVSTHWAYFNEFKEQFANTEVVDGAIITEERGIY
                     SSGGANSLWNLLFYLLEKYSDRHTAILASKYFAIDLDRNSQASFAIFNGQKNHHDTEI
                     LKAQQYIEKHTDAKLTIDQLADYTKMSRRTFERRFLKSTNHSVLEYIQRVKIETAKRK
                     FESSRKNITEVMFDVGYTDTKAFRDLFKKHTGITPLEYRNKYSKISLKG"
     gene            30675..31124
                     /locus_tag="PKU82_01605"
     CDS             30675..31124
                     /locus_tag="PKU82_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ81597.1"
                     /translation="MQTNNVNLHRVFASPTEKVFKAFSDADAYTAWLPPYGFVAKTHQ
                     YDFKVGGSYKISFINFTTGFSHSFGGEFVEIKLNDLIKITDRFDDPNLPGEMITTVHF
                     KKESCGTEINISQTGIPVAIPSEMCYLGWQDSLDKLKRLVEPNIPDA"
     gene            31191..31595
                     /locus_tag="PKU82_01610"
     CDS             31191..31595
                     /locus_tag="PKU82_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HOZ81598.1"
                     /translation="MSKQVFINLPVKDLKRSMSFYEAIGFTNNPQFTDHTAACMVWSD
                     TIFVMLLTHDKFKQFTSKLIIDAKQSVGVSNALFFESIDAMKSFAENAVKAGGREYAA
                     ASDYGFMQQHFIEDLDGHNWEPFYMDMSKFPG"
     gene            31616..32086
                     /locus_tag="PKU82_01615"
     CDS             31616..32086
                     /locus_tag="PKU82_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ81599.1"
                     /translation="MAKKKIAPCLWIEKDAKKAAKYYLSIFKEGKMLSYKAYKNSGTP
                     SGDFDIAYINFLGMDVQILAAGPLFKFNEAVSFVIICKDQKEIDYYWKALTKNGGSEG
                     PCGWCKDKYGLSWQVAPELYFKLEHHKNKAKQEYAFKALMKMKKIIIADLVEKK"
     gene            32117..32524
                     /locus_tag="PKU82_01620"
     CDS             32117..32524
                     /locus_tag="PKU82_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ81600.1"
                     /translation="MNKITVSAIINAPIKKVWDCYTNPNHIVNWNFADPSWHCPSATN
                     DMRIGGTYKARMEAKDGSFGFDFEAVYTQIESEKYFSYEFGGRVASVDFKNNDSQTEV
                     VVVFDAESENLVEMQRAGWQAILNNFKNYTEHN"
     gene            32528..32926
                     /locus_tag="PKU82_01625"
     CDS             32528..32926
                     /locus_tag="PKU82_01625"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ81601.1"
                     /translation="MQLCAYLHFNGDCRKAMTFYKKCLGGRLSFQMVSELPQSESLPT
                     YIKRNILTALLQTEYFQLAGTDLGNEAVYSPDKSFSLLLECRSKPELKRLYKQLSAGG
                     VKTQLLQKNFYGVIIGSFKDKFGINWVLQY"
     gene            33071..36049
                     /gene="dnaE"
                     /locus_tag="PKU82_01630"
     CDS             33071..36049
                     /gene="dnaE"
                     /locus_tag="PKU82_01630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOZ81602.1"
                     /translation="MHLCCHSYFSLKYGVLSPDDLLCIAAQKNIQSLALTDINNTSGI
                     LDFFRLAQRYNVRPVAGIDFRNGVEQKFIGIAKSNEGFHELNKFLSEHLISGEKFSDI
                     APAFQQAWIVYPMLSAPQQLHENEFVGIKPNELFQLNFSEWKSKLHKLVALAPATFRN
                     KTDFNSHRLLRAISYNTLLSKLPVSGQASADEIMYSPEELKCIYREYPQLIYNTEKLL
                     EPCCIDFEFGKNKNKKYFLGSAKRDHHKLMSLCYEGLSYRYPNTDDTIIDRFEKEIQM
                     IGELGFSSYFLINHDIVRYARSKNYFYVGRGSGANSMVAYLLRITDVDPIDLDLYFER
                     FINPQRTSPPDFDIDFSWNERDDVIDYIFNRSTFGKEHVALLATYSTFQRDAAIRELG
                     KVFGLPKAEIDSFFENGNTKRDHISNLVLQYAQRIIDVPSHLSIHAGGILISEKPMTY
                     YTALDNPPKGFPLTQFSMLEAEDVGLYKFDILSQRGLGKIKDAVAVIEKNRQVKVDIH
                     DIKKFKEDEGVRQKLQQANLMGCFYVESPAMRMLLKKLRAKTYLDLVAASSIIRPGVA
                     QSGMMREYILRFHEESRRTYIHPMMEELMRETFGVMVYQEDVIKVAHYFAGLTLTEAD
                     MLRRGMSGKYRGRAEFEKVKDAFFNGCRKKGHADKIAEEVWRQIESFAGYAFSKGHSA
                     SYAVESYQCMFLKTYFPLEYMVACVNNGGGFYAAEFYVHEVRMCGGTIHAPDVNKSFY
                     ETSLYGKNIYAGFHLVHELEMLTVTNILKARAAEGQFISLADFMQRVEISLEQLRLLI
                     RVNALRFTGRTKQQLLWDIHEIMGHDKKTNTEKQLFEAEHKHFVLPELHYSKIDDVWD
                     EIELLGFPLCSPFDLIKQKTNVTEGIVIHTAAELSAYCGKIISIAGYFVTRKPTRTKH
                     GQPMAFGTFLDSKGQWIDTTHFPDVVKQYPFRGRGCYRITGKVVQEFGFYSIDVISLY
                     LLEYKTRYAVVEEDKVHVNE"
BASE COUNT        11605 a         6375 c         7125 g        11154 t
ORIGIN      
        1 gcatgcattc accaatcctg aagctactgc agttggaaaa aagtttgata tgccaattag
       61 ttataatgcc gaagcagata aaaaatcatg gttggatatg aaagcctttc ttgcagagca
      121 tctgaaaaaa taaattattg ctttgaagca cttaatgcat atacaagagg caatttgttt
      181 tccatgcctt ttatcataaa gtgattttta cttacttcaa ccatgttgtt taaacagttg
      241 tagggagaat aatcaaactc attaaaagat tcaattctta atccgctgtt taatagacta
      301 ctcattacct cactcagact gtgattccag gtaactgatg tagtagtgat ttttgcttgt
      361 ttatcggcat aggttccttc atcagtttca ataatgggag cacttttaaa ataactgtac
      421 tgtacatatt taaaatcatt atcaaacatc caaacagcag gatgaaattc tgccatgaca
      481 aacacacctc ctggtttaag aaaagatgaa ataatatcag cccatttttg catatcaggc
      541 agccagccga tgacaccata agaagtaaaa acaatatcaa acttctgatc caaaacagta
      601 tttaaactat acacatcact gcatacaaat ttgcaacccg ttcctgattt tttattcaat
      661 tctatagctg tttcaattgc tttttcagaa aaatcaacag cagtaacttg cgcacccaaa
      721 cgtgctaaag aaatgctgtc ctgcccaaaa tgacattgca aatgcagaat tgttttacct
      781 ttaacatcac caagcagatt gagttcaatt tcattcaaag aattttttcc tttaagaaat
      841 gtctcgttat cataaaatgc agaatctaca tgaacatttg tgcgttcatt ccagagtttt
      901 ttgttcaaat ctaaataatc cataatgtaa aaatagatgt tcggcacaaa taccggaata
      961 ttgttcaata catcttatct ttgctctgta aattaatcgg aagagattat gtcgcacatt
     1021 ctactcattg aggacaataa agaagtaaga gaaaatactg cagagattct tgaacttgca
     1081 ggctacaccg ttacaactgc cagcgatggt aaaaaaggta ttgaagcagt tcagaaaaac
     1141 aaacctgcat taattatctg tgacattatg atgcctgtgt tagatggata cggtgtgctg
     1201 catctgctgg gtaaaaatcc tgaaacctca actattccgt ttatttttct gacagcaaaa
     1261 acagaacgtg gcgatttcag aaaaggtatg gaaatgggtg ctgacgatta catcacaaag
     1321 ccattcgata aactcgaact gcttaatgcc attgaaagtc gtttaaaaaa ggttgaagca
     1381 ttaaaaaaag aatattcaaa agaccttgaa ggacttacca attttattgg tgaagttggc
     1441 ggcaaagacc acatgcaggc atttattgaa aacagaaaaa tcagaaccta caagaagaaa
     1501 gaaaatatct ataaggaagg cgactatccg cgtggtatgt attatatcaa ccatggaaaa
     1561 gtaaagcttt ataaaagcca tgcttatgga aaagagttaa tcaccaacct gcttaaagaa
     1621 ggtgattttt ttggttacac ttctctcatc gaagaaggcg attatgtaga aagtgcacaa
     1681 acacttgaag acacagaagt ggtttttatc cccaaggaag attttctgaa tctccttttt
     1741 tcgaatcgag aaattgcaaa acgatttctg ctgatattga atcaaaagtt taatgagcag
     1801 caggattacc tgatagaatt ggcatatagc tctgtgagaa aacgtgtggc agaagcattg
     1861 attttacttt ccgacaaata caaagccaat cgcgatgaac gtttcagtat gtcaatttcc
     1921 agagaggatt tggccaacat tgttggtaca gctacagaat cactcatcag aacactaagc
     1981 gactttaaag aagaaaaact cattgaggtg aaagacggta aaattgtctt aattaatgag
     2041 aataaattaa aggagttgaa gaattaagca ttaaccatgc agtcaatact taattctatt
     2101 ttctatttct tagctgctgt ttcctttatt acttttccat tcttgtataa aatggtttta
     2161 agtaatttac catcttcgta aacccgccat tcgccttctt cattgttgtt tacatagttg
     2221 ccttcacttt gtatttcgcc attggcataa aaaatggcag ggccgtttaa gtcaccattc
     2281 ttgtaggtat attgtatggt aggtttgtcg tcctgtttaa accaaatggt aataccatca
     2341 cgaattccgt ttttataagt tgctttttct ttcagcttcc cgttttcgta ataatcaaat
     2401 cgcattccgt ctaattttcc gttattgtaa ttttcagagg taattagtgt aacgccatat
     2461 ttgtaaacat tacgcaggcc atgtaatata cctttgcgat aggattcctc atcttcaatg
     2521 taaccgttct cgttaaaatg cagatgtagt ccgttgagca catcagcttc aaattcttca
     2581 agcagttcta tttttccttt ggtatcataa aaacgccaaa gtccttcttt cttgccattt
     2641 tgaatttttc caacggcacg ggcccttccg tcaggaaagt ttattcgaca gtcaaataat
     2701 ccggccttgt cggggtctgg agtcaataca gtatcttgct tagaagtttg tgctcctgca
     2761 aatgataatg atagtccaag agcaatggtc aaaagtgatt taaacataat gcttaatgtt
     2821 atggcttgca aatctctaaa aaaatgatga aaagcagatt atagtagcat cagcagctaa
     2881 tgcttatttt tgcaggcaca atgacaacac aggaaatatt agaaaagtta gaagcgctga
     2941 aacatcgttt tgaagaaatt ggtcagcagt tgagcgaccc ttctgccaca acagatatga
     3001 gacgctttca gcagttaagc cggcagtata aagaattgca agaggtagta gaaacttatg
     3061 aacgatataa aaaagtaatt gataattacg atcaggccta tcatgtttta aatacagaaa
     3121 aagatgttga tctgaagcag atggcaaagg aagaaatgga aacgctggaa gcagaaaaaa
     3181 atagtcttga agatcagata agacttttac tgattcctaa agatcctatt gatgaacgta
     3241 atgcagtggt tgaaattcgt gcaggcaccg gtggtgatga ggccagtatt tttgcaggcg
     3301 atctttacag aatgtacagt cgctattttg agaaaaaggg atggaaggtt gatgtgattg
     3361 atgagaacga aggaacagca ggaggcttca aagaagttat tatggaagtt agtggcgatg
     3421 atgtttatgg catcatgaaa tatgagagtg gtgtacatcg tgtacaacgt gtgcctgata
     3481 cagaaactat gggtcgtgtg catacttctg ctgctacagt tgtagtgttg cccgaagcag
     3541 atgatgtaga tgtagtgata aatcctgcag atttggagtt tcagacagcg cgctcaagcg
     3601 gtgccggtgg gcagaatgta aataaggtag aaacaaaagt tcagcttacc cataaaccta
     3661 ctggcataat ggttacctgt cagattgaac gttcgcagca tggaaaccgt gagagagcat
     3721 tgcacatgct caaaaccaaa ttgtatgaaa tagagttgca gaaacatgag tcggagattg
     3781 ctgccagacg caaaacaatg gtatcaacag gtgatcgctc agcgaaaatc agaacataca
     3841 attatccgca gagcagagtt acagatcatc gcatcggttt gtcaacttac aatttgtcat
     3901 cttttatgaa tggcgatatt caggaattta ttgatgcatt gcagttggcc gagaatgcag
     3961 agaaaatgaa agaggcctca ttaagttaag ctatgaacta tcagcagtta gtaggcgaaa
     4021 taaaaagaaa acaatctttt ctctgtgtag gtttagatac agacttaaaa aaaatccctt
     4081 cacacatcag agataaagca gacgatcctg tatttgaatt taacaaagca attattgatg
     4141 ccacattaga ctatgcaata gcatacaaac ccaatcttgc attttacgaa gcgtcaggtg
     4201 ttgagggact gaagagcctc gaaaaaacaa ttcagtacat caatggcaga gcatttacca
     4261 ttgccgatgc aaagcgtggc gatataggta acactgctga aatgtatgct catgcttttt
     4321 acaatcatta tggttttgat tccatcacac tctcaccata tatgggtgca gacacgattg
     4381 aaccattctt aaaacataaa gataagtgga gcatcattct tgcattaaca tcaaatcccg
     4441 gagcagaaga ttttgaaatg cttaaagtgg atgatgattt tttgttcgag aaagttttga
     4501 aaaaatttag caaggcttca gcgcaacaga taatgtttgt tgttggtgcc acacgtccgc
     4561 aatattttga aattgttaga aaacatgtgc cagacaattt tctgttagtc cccggcatag
     4621 gtgcacaggg tggcagtctt gaagatgttt gccgctacgg actgaattcc aatgcaggat
     4681 taatagttaa ttcttcacgt caaataattt atgcagacag cggaattgat tttgacaaaa
     4741 aagcagctga acaggcaaaa caaattcaac aacagatgaa acagatgctt gttgaattta
     4801 aaattacagg ctgatgccat ttaccttagc gcatcctgca atagtaattc ctttttacag
     4861 actctttaaa gagcgtctgt cgctgacagg tttaattatt gggagtatgg ttcccgatgt
     4921 tgaatattgt gtaaacacag taacacgaag tgtaatcagc cacactgaaa aaggtatttg
     4981 ggtgtttgat ttacctgtag ccatttttct gactttttgc taccatggat tcgtcaaaca
     5041 aattttaacc aataacctgc cggattggat tcgctataga ctttctcctt atgccaataa
     5101 aaactggttt cattatttca gaaagaattt tctgaattat tttctctgtc ttcttgcagg
     5161 aattgtttta catctgctgt gggattctgt ttcgcatgga actggatatt ttgtaagacg
     5221 aacagcattt ttacaagaga atgtaatcgg tacattaacg gttacacgat taatatggca
     5281 cataagtacc gttgccggcc tttacgtagt tatccgattt tttctgagtt ttcccgtcaa
     5341 caaaatggaa gaagaaaata atataccaga taaaaatttc tggccgttgg tttttttagt
     5401 tgcatcagtt ctcactatca ttcactggct gccttttgtc agaccgcagg aaccacgttt
     5461 tattttagtt gctttttcag gtgcaatgat ggttggagtt attgtagtat gttttatctt
     5521 taggtttttt aagaaacaac tgtcatgaat gaagcattgc tttatcatct atggaagtac
     5581 aggctattta agtctttgcc gctaactact attgatggta agacaattga ggtaataaaa
     5641 cccggagaac tcaatcccga ctcaggtccc gattttttta atgcccgtct gattattgac
     5701 ggaacagaat gggcaggcaa tgtagagatt gaccctacaa gtgctgattg gtacaaccac
     5761 aaacatcata tcaataaagc atattcaaag ttaatactac atgttgttta cgaaatgaat
     5821 aaaccattag atcatggttt tcctgtgttg gagtttaaaa aatttgtgga cgatggagct
     5881 gtaaaaacat tccatcaatt gcaagaatcg ttattgccaa taccatgcag caaacaagta
     5941 catcatgtaa acgatgtaac gttaaacacc tggcttgaaa gaatgattat agaacggtta
     6001 gagcaaaaaa ctactgttat tcatgacatg ctgcatgcta ataagtttga ctgggaagag
     6061 acattttatt tttttcttgc ccgtaatttt ggatttaaaa ccaatgcact gccgtttgaa
     6121 atgcttgcac gttctattcc gatgaatgca cttgcacgac ataaaaataa cttattgcag
     6181 attgaagcat tgctgtttgg acaagcggga tttctcaacg ataaacatgc tgatgaatac
     6241 attgaatccc taagacgtga atatcgtttt cttgcaggta agtttcagtt gcaggcaatg
     6301 aacaatagct tatggaagtt tgcacgaatg catccacgca attttccttc tgttagaata
     6361 gctcagtttg ctgcactcat ccataacagc acacatttgt tttcaaaaat attggaagag
     6421 aaagatgtta aatcgcttat tcaacttttc agcatcacac cttcttctta ttgggataag
     6481 cattttcatt ttggaaagga atctgcagag agtaaaaaaa cattcggacg cagttcagtt
     6541 gacaatatta ttatcaacac tattgcacca tttttatttc tatatggtaa aattaaagat
     6601 aatgaaaaat taaccgaacg tgcattatct ttgctcgaac atatcaaacc agagaataat
     6661 gtaataataa ggcagtggaa agaagtagga attacagtta aacatgccta tcatacgcag
     6721 gcattgattc aattgaaaaa tctatactgc agtaagaaaa aatgtttgag ttgtgcttta
     6781 ggagttaaaa tacttaataa taagaaaaat gattattgac agcattaaaa atttttttga
     6841 aagacaggct ttcggtatct gtgagtattg gggcgaacgt ctaaaaataa gatcatcggt
     6901 catcaggttg ttttttattt atctttcttt tctgacaatc ggttcacccg tagttgtgta
     6961 tatggtgctt ggcttctgga tgaatcttag aaaactcatt caccgtcaac gtggaggtgt
     7021 gtgggattta tagagaaatt ttgcagtaat agatttagtt ttttacagaa tgataaaaat
     7081 gtacattaag cccacttgca gcacctgtcg cactgcaatg caaatggcta aggagtgtga
     7141 gacagagata gaaatgatag aatacctgat tgatacacca tcagaaaagg aattgctgga
     7201 gttaacacaa caattgggag taaaaccatt tgacttagtc agaaccaagg agtcgctata
     7261 taaagagaag tatgaaggca aaaaaatttc agataaacaa tggctgaaaa ttttgcataa
     7321 gaaccccatt ttaattgaac gacctataat tactgatggc actcatgcaa tcataggaag
     7381 accaccaaca ttaattgcgg acttcctgaa taagaagcaa aagacgaaaa agaaatcaaa
     7441 gtaaactgtg ataaaaattt tagtttgtag gagtagaatg caatacgtta ttcaaagatt
     7501 atttcttttg gttttgatgc tgtcagcaat tcaggtcaat gcacaacacg atgatttgtt
     7561 tattccccga aatattcaaa aagcatatca gaacaataca agaacaattg aaggtgcacc
     7621 aggaaaaaag ttctggaaaa attttgcaga gtattttatt caggtaaagt ttaatcctaa
     7681 aactttaaaa atagaaggaa agcagattat aacttactac aacaattctc ccgatacttt
     7741 aaaaaaattg gtgtttcgta ttgcccccga cttttataaa aaaggaaatg aacgcaatcg
     7801 tattattgaa ccagacgatg ttactgatgg agtaaaattt actaattgtg ttattgatgc
     7861 aaacaaagtt gatcttaata atcccatgct agtaaaacac gaaggaacaa taatggaaat
     7921 taaggttaaa ggtattgatc ctcgtggtgg cagggcaact gttgagatgg actggagcta
     7981 tacactcaat ctgaactcgc atgaacgaac cggagtgatt gatataggaa gttatttcat
     8041 tgcatatttt tttccacgta ttactgttta tgatgatgtt gatgggtggg atgaattccc
     8101 ttatcttgga caacaggaaa cctacttcga caatgccagc tttaatgtcc acataacagt
     8161 gccaagaaat tttatggtgt gggctacagg tgatttgcaa aaccctgcag aagtgctgca
     8221 tgaatccgta ctaaaaaaat ggactcttgc acagcaaagc aacaaacctg tttttttaat
     8281 tgacagtgca gatattcaga ataaaactgt aacagtgaat aaaaaattca acacttttca
     8341 gtttaaatgc actacacctg attttgtttt tgctgtaagc aatcattact tatggcaggc
     8401 acgtgatatg gttgtagata gtgttaccat gagaaaggca ttaatttcaa cagcatttaa
     8461 tccaatacat aaagattttt ttgaggtgaa tgattttacg gcaaaaacta ttgaagtgat
     8521 gagttttgat tttccgggtg tggcatatcc atacagtcat cttactgttg ttgacggacg
     8581 tgatcagatg gagtatccga tgatgataaa tgacaatcct actactacac gttttgacgg
     8641 aattacattg acaacacatg aagtctttca tcagatattt ccgtttatga tggctaccaa
     8701 ccaaaccaaa tatgcattta tggatgaagg ctgggctaca attggcgagt ggtatatcag
     8761 tccgataatt gacagtacta ttgttgacga ttatggaatg gatagaatta actccgattt
     8821 aggtaaagac tgggatacac caatgattac caattctact gagttgacga aatcattatt
     8881 cattaacagt tatcccaagc ctgctctcac atttttatat ttatgggaga tgatgggcga
     8941 ttcattattc aaaaagtcat tgcagcatta catcattacc tggatggata aaagccctac
     9001 tccatgggat ttctttttca gcatgaacca aagttgtgga atgaatttaa attggttttg
     9061 gaaggcatgg tattttgaac aaggctatcc tgatttaggg attattggta ttaaccgtct
     9121 taactatagt tacactgtaa ctattaaatc tattggcaac aaacctgttc ctgtacatct
     9181 cacagcaaca tttgcagatg gaacaaaaag gaaatttaca cgaacagtag atatttggaa
     9241 aaacagaaac atcaccgatg tgataattga tacacaacag tcaattacta agttggagtt
     9301 gggcgatgtt tatgatgctg atgtagataa aaacaataac gtgttagttc ctaaagagtg
     9361 aggaattaat ttaattttgc agacgaaaaa aatggtcccg tagttcaacg gatagaatag
     9421 aagtttccta aacttttgat ggcagttcga ttctgcccgg gactacgaag aaaaggcagt
     9481 tccctgtccg actacgaagt aggcggagat tctgccagag actacgaaga aaaggcagtt
     9541 ccctgtccgg ctacgaagta ggcggagatt ctgcccggga ctacgaaaac agttatgcaa
     9601 atgccatatt atgtttatgt tataagaaac aaggtaacgg gaagacttta taaagggcaa
     9661 acagaaaatc ttgaaaagcg gttgaaagca cattcgcaat cacaaacaaa gaccacaagt
     9721 tactttccaa aagattggga gttggtttat cacgaaattt ttagttcaag aaaagaagct
     9781 gtagagaggg aaaaatattt taaaacagca gcaggcagaa gatatttatc tggaagaata
     9841 gcaaatatta aatcaacgga tacccgcccg aacgtaccgt tcggtacggg cggggaatag
     9901 aagtttccta aacttttgat ggcagttccc agtccgacta cggagtaggc ggagattctg
     9961 cccgggacta cgaagaaaag gcagttccct gtccgactac caagtaggcg gagattctgc
    10021 cctgaataaa aaaacagttt tagaaatgct atatattaaa gattaaattc attttgtatg
    10081 gaaaactaaa aactgatttc ctattcaata accacaattc aacaaacatt cacaaactat
    10141 aaaggagaat aattatcttt gcggaatagt tataaataga tatttttatg catatccatt
    10201 tgtttcgtga acagttaaaa caaaaaggac taaaagttac accacagcgt gtagcaattt
    10261 atgaagctgt tgcagaactg cacaaccatc ctactgcaga gaatattatt gagtacatta
    10321 aaacaaatca tccgaatatc tctgtaggta cagtatataa agttcttgac tcattagtag
    10381 agaacaacct gttgcgaaaa gtaaaaaacg aaaaagatat tatgcgttat gatgcagttc
    10441 tggagcatca tcatcatttg tattgcgcag aatccgatcg tatagaggat tttgaagacc
    10501 cgcagttaga tcatttcatc agtgaatatt ttaagaagaa gaagataaaa aacttcaaag
    10561 tgcaagacat taaattacag attacaggaa ctttcaaata aaaaaataaa acacacatat
    10621 tatggacaac acaaaaagcg gcaaatgccc gtttcatcat ggagccaaca ccgaagcaag
    10681 tacaggtgtc atgaactggt ggcccaaagc attgaactta gatatcctgc atcagcatga
    10741 tacaaagact aatcctaatg gagaaaaatt taattacgca gaagaattta aaaaattaga
    10801 tttagaagct ttaaaatctg acctgaaaaa attgatgact gaaagtcagg aatggtggcc
    10861 tgcagatcat ggaagctatt cgggtatgtt tgcgcgtact gcctggcact tagcaggcac
    10921 ttacagaaaa gctgatggtc gtggtggtgc aaacaccggc aatcagcgtt ttgctcctct
    10981 caattcatgg cctgacaatg tgaatacaga taagggaaga agattgctat ggcctatcaa
    11041 gaaaaaatat ggtaacaaaa tttcctgggg cgatcttatt gttttagcag gtaatatggc
    11101 ttatgaagtt gcaggactaa aaacatttgg ttttggtgga ggtagagaag atatatggca
    11161 tcctgaaaaa gatatttact ggggatcaga aaaacaatgg cttgcaccaa ctggtagtga
    11221 aggcacacgt tattccggtg aaagagactt agaaaatcct ttggcagctg taatgatggg
    11281 attgatttat gttaaccctg aaggtgttga tggaaaaccg gatccattaa aaacagcgca
    11341 agacattcgc gttactttct cacgaatggc aatgagtgat gaagagactg ttgcacttac
    11401 tgccggtgga catactgtag gcaaagcaca tggtaacgga aaagtagaag tgttaggtgg
    11461 tgttcctgaa gctgcagaaa tacaacatca gggattgggt tggtttaatc ctgatggctc
    11521 tggcaataaa gataaaacta tggcaagtgg tattgaagga gcatggacta cagacccaac
    11581 aaagtgggat aatggatttt ttgatttgtt attgaactac gattgggcat tgacaaaaag
    11641 ccctgccggt gcatggcagt atgagccaac aaatatgcct gaagaaaaga aacctgttga
    11701 tgcatttgat ccaaaagttc gcagaaatcc tatgatgact gatgctgata tggcattaaa
    11761 gatggatcct gaattcagaa agatatcaga gagattttat aatgacttca attatttttc
    11821 tgaaacattt gcccgtgctt ggtttaaact tacgcataga gatatgggac caaagtcgcg
    11881 ttaccttggt gccgatgtgc ctaaagaaga tttgatttgg caagatccta ttcctgcagt
    11941 tagttacaca ttgtcagatg ctgaagcaga tgaaataaaa actaaaattt taaactcagg
    12001 tttaagcaga acagaattga ttaatactgc atgggattct gcaagaacat atcgtggatc
    12061 agatttcaga ggcggtgcca atggtgcaag aattcgtctt gagccaatga aaaattggga
    12121 aggcaatgag ccgaaacgct tacaaaaagt gttggataaa ctcacagaaa ttcaaaaagg
    12181 attaagcaag aaagtaagta ttgcagattt aattgtgttg ggtggtagtg ctgctgttga
    12241 gcaggctgca aaagatgcag gcatgtcagt aaaagttcct tttgctgccg gcagaggtga
    12301 tgcaacattg gagcagactg atgtagattc tttttctgat ttggaacctt tacatgatgg
    12361 atacagaaat tatcagcaaa aaaactatgc tgtttcttca gaggaattgt tgttagacag
    12421 aacacagtta atggggctca ctgcacctga aatggttgtg cttgttggag gaatgagagt
    12481 tctgggaacc aacttcggtg gaagcaaaca tggtgtgttt acagagagag aaggtgtttt
    12541 aactaatgac ttctttgtaa accttactga tatgaaatat gagtggaagc ctgtttctga
    12601 aaatctgttc aatattgttg atagaaaaac aggagctaca aaatacacag ctacacgagt
    12661 agatttggtt ttcggttcca attcaatttt aaggtcatac tcagaagtgt atgcacagga
    12721 tgataacaaa gaaaaatttg tgaaagattt tgtgaaagcc tgggtaaagg tgatgaatgc
    12781 tgacagatat gatttgaaaa aataatattg tttagtactc caaaaatgcg agcagcctct
    12841 gaaaagaggc tgctcgcatt ttatatcatc tgcttaactg taaccactaa agttttgaag
    12901 caaccacaaa agctttttta tactttttag tttttccttc gggattaaat gcttcggcat
    12961 aaacaatata aattcctaca gaggctttat catttttatc ggttatgcca tcccacataa
    13021 aactatcttc cggtgctaat agttttgatt ttaccaagtt gcgaaccaat tgtcctgctt
    13081 cattataaat ttttgcattc agcatataac ccggttcatt catgttgatg ttaaatgtta
    13141 ctacatcatt atatccatcg ttatctggcg aaaaaatttc tggggttatt gtaaaattat
    13201 tttcctgtaa ttgttcgttt aaactttgtg agtttttata gcccggtgtt gcatagccaa
    13261 cagcttctgc tgccgaatgc cagttagtag aatcctgtgt gcctttatta aaccatattc
    13321 tttctaatga aacaccattc tgatcgtcaa tgagtggaaa atgccaacgc ttattatagt
    13381 taagctgatc aattctgttc aatgcaaaat cggtaatgac acaaacaccc atgtcatcat
    13441 taaaacccgg cagtgatgct tttagtaaat tatttgttgc agcattggga taaaaacttt
    13501 gcagtgatgc aacatcagaa gtaagtgcta tgtattcctt cggaaataaa agataccctc
    13561 catcactaac actataattt gttgttatca gattgttgtt atcagttgtt gcaactttaa
    13621 gatttttaag gtcaattatt tttgatgagt tgttgtaaag ctccacaaag tcatttcctc
    13681 ctgtttttgg gttaaacaat atttcgttta tcagcacatc acctgcaaca gcagtttctg
    13741 caataccaat ttttaaacta agttctcctg ttattttatt tcccggacag tcagcaattg
    13801 atgagtttaa attaacatga taaactattc cggtttgcaa tgcattattt agcgttaaaa
    13861 aggcatgatt gtctgaaagt aatactgatg caggctgacc tattccattg tcaataatat
    13921 aacttgatgt ttgtgatgca atagcattgt caggattctc gctaaatgtt attcttatca
    13981 atgttgtggt aaggggataa gcatcaactg cctgtggtgg ggtggtatcg ctgtaagtac
    14041 ctttaacact gtttactttt cccggtgttc cgccttgcgc atcatttgat gcatgccagt
    14101 taaatcttga attacaagta aaatcagcat caattctttc aatggtgaag cctcctgttt
    14161 ttttgctgtc atcgttgtag ttatcatcac tgaaaattac tctgtctatc aatgtgccat
    14221 tgttatcagt cagtttaagg tcatcaccaa catcattatt taacgaagga aatgaacttg
    14281 tccctgttgt attaccatag ggaataaatg atgttgtgtc agaattttta cagacaatga
    14341 tatatgcact tggttgaagt gttactgcgc caatacttgc cacggaggtt gacccgtcag
    14401 ataatgtcca gccattgagt gagagaattt tgttacttct gttgtacagc tcaacaaact
    14461 ctgcattagg cagtggtgcc gacacaatag gttcaaaata tatttcatta atgacaacat
    14521 caaaaggctg tgctgttaca ggagcattcc atgtaaagcc tatagtgctt cctgcagcaa
    14581 ttacattgct attaagatca gatacattac ttacagagag cgtgtatgca gtgccacttg
    14641 taaatggagt gctaaatgta agatgtacta atgaaaaatc acttatatcg cgtactgcag
    14701 ttgccggagt gcccaaaccc tgaattgcat aattcgttgt agtctgtgat gtagtcaggt
    14761 caacaagctc agaaaatttt acatctaaag cagtattcga taacggaata gcggaagtta
    14821 gtgttggtgg tgtgatgtct gcaataaatg gaatagagaa atcgtcaaag aagaagcctg
    14881 tggcattgga tgaagtgtaa gtacaactca caccaaaata acttcctgaa ttaattgttg
    14941 catcagtacc tgaagattgt aaagtaaaat ttgttcctgc agcagcatct gcatatatac
    15001 tccataggcc tgcattatct cttgtaattt ttacactgat agttgatgcc gctgcaataa
    15061 atccattggt gcctcgtgct actgatacag aggttgtacc tgtttgttta aaaatttcga
    15121 tggcatcgtt gctgcctgtt tctccaaact gtacataata gccgttgagt gcacctttta
    15181 agttttgttg atcgctcacc aaataaattc ttgcataatt acttgacgat ggcgcaaagt
    15241 tgagctttat ataaaattgc caagatgtat tatcgagtgt aggcaggctt gttgttaatg
    15301 ccagatagga agttgctgca ccggtgctgt tgagttgcag ttgctgtgat gcattgacta
    15361 taaactgact gttgtcgcca ctccatgcag gattagcagt aaaatcacca tcagaaaagt
    15421 tatctgttat ttgtgcaaca ccaaaaaatg gaatcattgc gaaaaaaagt agtaatcgct
    15481 tcatgttaaa ataaaatata aggcagtgaa gataagcaaa cctcattatt taaaataaat
    15541 aaattgaaac agcattaagt aatgtgtttt ttattcattt ttgaggaagg aataaaatat
    15601 tatgaaagta gctgtagttg gtgccacagg cttggttgga aaaaccatga tgcgcattct
    15661 ggaagaaaga aagtttccgg tatcggaatt tatacctgtt gcaacggcaa attctgccgg
    15721 taaaaaagta atgttttcgg gaaaggaata tacagtgata acttgtgaag aagcagttgc
    15781 tctgaaacct gatattgcct tattctctgc cggaggatca acatctttag agtgggctcc
    15841 aagatttgct caggttggaa ctactgtaat tgacaactca tcggcatggc ggatgaatga
    15901 aaaatgccct ttagtagttc ctgaaataaa tgctgatgta ttaactaaag aagataaaat
    15961 tattgccaac ccaaattgtt ctactattca gatggtgatg gtacttaatc cgctacataa
    16021 aaaatataaa cttaaaagga tagttgtttc tacttatcag tcagtttccg gaacaggaaa
    16081 aaaagcagtt gatcagttga tgaatgaacg caaaggaatt tccggtgcta tggcctatcc
    16141 atataatatt gacatgaacg tgttgccaca tgttgattcc tttttagaaa atggatatac
    16201 gaaggaagaa atgaaaatgg ttaatgaaac aagaaaaata atgcgctctg acaaaataaa
    16261 agtaacagca acaactgtgc gcgtaccggt tgttggaggc catagcgaag cggtgaatgc
    16321 agagtttgaa aatcagtttg aattgagtga tgtaagacaa cttttatcac atactgcagg
    16381 tgtgacggtt attgacgacc ctgcacaact tcagtatccg atgcccactt atgcagaagg
    16441 aaaagatgat gtttttgtag gaagaatcag gcgtgatgaa tcacaaccac aaacattaaa
    16501 cctttgggtg gtatctgaca atctcagaaa aggcgctgct accaatgctg ttcaaattgc
    16561 agagtatttg ctaaagcagt tttggcaaca ttaaagcttg taagtgataa aaattcaaat
    16621 tgtttacaat aaatacttta cctatttgac agattgaaat agaaaaaact tgatattgac
    16681 agttgttgac ttaaattaca atttgatttg aacagtcttg aatttatgtt aaattaacga
    16741 tcccataaag ttagatagtt ataagataaa ttaagtaatg agataacgga ctattgctaa
    16801 gggatatagt gattttttaa aactcaatta ttaaacagaa aaaatagtat atttgaattc
    16861 atgaattggc aggaatacat aatgtctgat gaggaagtac ttttagggaa accaaccata
    16921 aaaggtacaa gaatttcagt tgaacatatt gttgggctat tagctcaggg ttggacagaa
    16981 aaagaaattt tggaaaattt tcctcgattg acaaaagaat ctttgcaggc agttttttta
    17041 tatattcaag actgttttag tgacggatta ttatttactc ctaaaagaaa aacggcttga
    17101 atttaattta tgaaacttct agctaatgaa aattttccat taaaaagtat tgtttacctt
    17161 caaaacggag gatttgatat tacttccatt ggggctgaac actcaagcat taaagatttt
    17221 gaggtgatga caatcgccat taatgaggag aggactatat tgacttttga tagagattac
    17281 ggagaattga tttttaaaca caattacaaa ccacctaaag gtgttatata tttaaggtta
    17341 gaaaaatata gtgccgatga acccgggcgt ctgattgaag aattggtaag cagtaatcaa
    17401 tatatttttg atggtgcatt aacagtattg gatgaacggg gaattaggca gcgcaaatat
    17461 tagtagtttg ttaaattgta atatttttaa agtaatttat tctgcattgt atcttgtaaa
    17521 ttacaaattg gattcatcaa agcatacaaa aaaaagccac cccaaaaagg atggcttttc
    17581 tttcttaaaa attatttttt tagttgcgtc ttccgaagta aatcaagaca taatacatca
    17641 gcgttgctaa ggcacctaat gcagcaacaa cataggtcat ggcagcccac cacaaagcat
    17701 cttttgcttt tgcatgttcg ctgcttgtta ccatgcggct gtcgctaagc cataccaatg
    17761 ctctgcggct tgcatcaaac tcaacaggaa gtgtaataaa actaaacaac actgtcatgg
    17821 caaacaacac aatacctgca agtagtagcg atggaaaaac attgatgagt aaaacaccgc
    17881 caagcaatac ccactgtacc cattttgagg caaaacttac taccggcact aaagccgaac
    17941 gaacctgaag catggtataa gcttttgcat gttgcacagc atgaccacat tcgtgtgctg
    18001 caacggcagc agcagcagca ttacgtccat aatatacagg ctgactcaga ttaacggttt
    18061 tgttaagtgg attgtagtgg tctgtcaact gaccttcaac agaaataact tttacatcat
    18121 taataccgtt gtcatgaagc attttttctg caatctcctg accactaaga cctgttccta
    18181 aagtttcatg cgagtattgt tcaaactttc gttttagctg ggcgcttact aaccacccga
    18241 caagcataaa tactattact ataagatata ttcccattta ttgatttttt taaattttta
    18301 aactgctttt cactaatttt ttttaaccac aatcaaacag tgtgcaaata tacacaacct
    18361 gcatttttgt cagtgttttt tatgattatt attgaatgaa ttactcccag cgaaacgttt
    18421 tggcagctac aatgtaaata ataacacccc aaagcagcaa taccattaac tgtgaaatga
    18481 catctattaa tcctgcacct tcaaatgcca cttcgcgtaa agcatcatta aggtaggtta
    18541 acggcagtgc cttgcaaaac ggttgcagcc attttggaaa aacatcaatc ggaaaaaatg
    18601 ttcccgacaa caaaaactgc ggcatagtaa tgatgttggc aatgggtgga attgtgcttt
    18661 cactttttgc aattccggaa accacaaagc caaaacccat aaaaacgata agtccaaagg
    18721 cgcagataag catcattgta agaaaagtgc ttatgccatt aatgagtgta aacccaaatg
    18781 caaacctgcc cacacctatt atgaagcaag caccaagcaa tgaaaatacc attcggctta
    18841 atgtttcacc cagaacaatg taaatacgtt ttactggcgt ggcaaaaaac cgttttatga
    18901 ccaaggtttg tctgaggctg aaaaaaacaa atgcagttcc gaaaactcct gttgacaata
    18961 aggcaaaacc taactgaccg ggaagtataa aatctatttt cttgtattcg cgtcctgcaa
    19021 tttcttcatt ttttaactgt gccaatggat gtgctacatg tgcatcgtac aaatttattt
    19081 tgcttataag attatctaaa atgtttttag aaagtgctcc accttcaggt gcagctgtgg
    19141 aggtaataag tctggccgta taatctattt tgccttcagt aatatttttt gtgataacca
    19201 aaatcccatc aatctttcct tgctgaagtt ttttacttgc aatagcagaa tcgccttcaa
    19261 tccaattgat aacgtcaatg ttttttgtgg cttcataaat cggattatga tggtcgcttg
    19321 aaggggttag aaataatttg actttaagtc ctgtacccga taaaaatcca aaaaccaaaa
    19381 taaaggtaag cggaaaaacc agagtaaaaa ctactgcact ggggctgcgt gttattgacc
    19441 tgaaactggc tttagcaata gccagcatgg cctttgtttg agaataactc atgttgttta
    19501 tgtcagaaat gttgcaaatg tagttaagtt ggtttttatg aattaaaata aaaaatgtac
    19561 gtttgccgta ataacatagg ttcgtcaatt caatgaatca cacattctca ttatttcaga
    19621 gcatggagag ggataagatt atcctttctt tcaaaggaga tattacccag gatttgcttg
    19681 cttctgtata tcagattatg gagtcgagaa tggatcagga taagactgat tccaagagga
    19741 agaaaagatt ttatcacatt ttagttgaat gtcttcagaa tgtatatcac cacatggaag
    19801 atttcagaaa tgtgcatgag ctggatgaaa tttctgatgg ctctgcaatt tttatcattg
    19861 ccaaggcaaa aggtgagggc tacagaatta ttacaggaaa ctatatcaag aaagatgaat
    19921 cggaagacct taaatcgaag cttgagaaaa ttaattctat gaacaccgat gaactaagaa
    19981 cttattatct tgaaacacta agcactacag agctttcgca aaaaggtggt gccggtttag
    20041 gaatgattga tattgcccgc aagtcgggac ataaaatagt ttacagtttt gacggtgtaa
    20101 gcgacactgt taattttttt acccttgaag taacaattaa ttaaatcaaa gaaaatggat
    20161 aagctgatta ttgaaccggg cataaaaaca cctcacattg tgtttgacgc tgccaatgga
    20221 agtcttgaaa ttaaaggtaa atccattccg gaaaattcta ttgaatttta taagcctgtt
    20281 tacgattggc ttgatactta tacaacacaa cccaaagatc aaaccttgct gacagtgcag
    20341 ttagaatatt ttaacactag ctcttctaaa tgtttgctcg acttattcag gaaacttgaa
    20401 aacattcatt cttcaggcaa aggaaaagta aatgtaaagt ggctttatga ggaagatgat
    20461 gaagacatta tggaagccgg tgatgactat cagaccattg taaaacttcc tttcagtatc
    20521 gagaaaattt caggataatt attttccttc aaaaatttcg tcaatagttt ttacagcaac
    20581 tgcatgataa cccggacgtg ccgacttata aatttgttct atccaaggtc gtaatgttgc
    20641 atctgctgca gcttttcgat aaagtggtgc aataaatttt ctgcgtccta ctgtattcag
    20701 aaaattttct aatgctttat atgcaggctt gtagtgacag gcaaaggcta tcataaacca
    20761 atcaaatgca atttctgaat tttgaatctg agtgaaatga aaatgattat ctaatgcaac
    20821 agctttttcg taggtcattt cgggagcaat agttcgtaaa aaatgcaggt aatgatgcgt
    20881 tgtccatcct gcagtatctg gcataactgt tggctgtttg ataaatgctg ctgctgcttt
    20941 ctcaacctga tcaaaatatt tgcttttaat aacaggcaga ctcttaggaa gtccggttcc
    21001 ataaacccat tcatcaacat ttactttttg ttttatcaac ggattttcct gcaaaagatt
    21061 tacctccaga taatgtagaa attcttttgt tgtcatggtt tgaaagcgat gttcttcaaa
    21121 atactttttt aaaaatgcat cccacatatc acggccggct gcttcttcta atgccatcag
    21181 aaaaaaacgc cctttttcat aggcaatatc acccatgcca tcatcaggat cacgaccttt
    21241 aaggtcaaga aaaagtttag tatccggatt gttaacaccc atatcggcaa cggtatgttt
    21301 caattcattt tgtgaaagta agttcagcat gtcagcataa tctttaccat aaattttttc
    21361 cataatgcgc gattcaaagt aaactgtaaa tccttcattt agccagaagt cattccatgt
    21421 tgcattggta actaaattgc cactccacga atgtgcaagc tcatgtgcaa ttagactcac
    21481 taacgaacgg tcgcctgtaa tgatggtagg tgttgcaaat gttaaacatg gattttccat
    21541 tccaccaaaa ggaaaagaag gaggcagcac caaaacatca tattgtagcc atgggtattt
    21601 accatacaat tgttctgccg aagaaatcat agcaggcaaa tctgtaaact catgaaaagc
    21661 tttggccaac ataacaggtt ctgcgtaaat gccggaacgg ttgtcgtagg ctttgtaagc
    21721 aaggtcgcca atgcttaaag ccataagata ggaaggtaca gcaaaaggca ttttaaatga
    21781 ataggtgcca ttgccaattt gtttaggatt ttgtggacaa ctcatcaatg ccataagttc
    21841 tttaggacaa tgaatggttg ctgtccatgt aaattttatt ccaggtgtat cctgcaaagg
    21901 tatccagctc cgagccagaa tagcttccga ttgtgtatat aaaaaaggat gttttttgcc
    21961 tgcagtttgt tccgggttta gccactgtaa tgcttgtgca tcaggagaag tttcataata
    22021 aatggtaacc tcttttgtat ttgctgttat aggaatagca agttcgctgc ctaaaatttc
    22081 atctgcaggt tttaaaataa atgtaacagg agcatcattg atttttacac tttcaatttt
    22141 caggttgtag gtgtctaaac gcaaagtatc ggcattggta agatttttta cttgcaaagt
    22201 cacaaagcca actaactttt tagttgagaa atcagcttta aggcttaagt caagatgctg
    22261 tacaacaatt tctgctggat tggaatatga atgttgatct ttaacgtata tccctctttc
    22321 atcttccggt tttttagcaa acatggtaaa tggtattaga attaaaaata aaatattttt
    22381 cattgttcaa ttattttgtt ttaatcatat aaatatgagg aatgttatct tccagaaatt
    22441 cttcgctgat gacaacaaac ccaaatgact cataaaattt acgaagataa ctttgtgcca
    22501 taatttttat tgcactatta gggaataagc ttttacattc atctaaagat ttttcgacca
    22561 ctaattttcc caatcctgtt cgtctgagtt gttgatgtgt aacgatgcgc ccaacagaaa
    22621 cttcatcata caaaacaccg ggaggaataa tacgtgcata accaacaact tgttgatttg
    22681 ccatagccat cagatgaaaa gaaagtttat cggttttgtc aatgtcacca taaggacaat
    22741 tttgctccac aataaaaacc tgctgcctga gttgtaaaaa atcatgcagt tcatgtgcag
    22801 tgaggtcatt aaacgattta aacttccaaa aaatattcat gctgtgatgg gctgatatgg
    22861 cagcgaatat aaaacacatt ttatgaatag taaaagaagt aatacgattg aataaattgt
    22921 tagcagcatt ttgatgatta acactattta catcaaacta aataaataac tttggcgcca
    22981 tgtcattgcc acaactcatt gttactgaca gcagccgaaa cggaaaactt ccatttgctc
    23041 cttttaaaag gacacaggaa gaatctgcac aacagccaaa gacagagaag gtaagcaaca
    23101 aagttgcgaa gcagaaactt tctttaaaaa cagacagctt aaaaggtaat accactgtag
    23161 ttgatacaat aaaaccaata gcaaacgata gtattcaact tgcaattgat tcttcagttg
    23221 cagatatgtc tagattgttt cgttttacag atgaggaaaa tgatgaactg aacaattcag
    23281 atcaacaatt tatttcatca tcggcatatt tcaaacagca gcagttggaa gtaaaatcgt
    23341 catctccaca attgtttaat aagatagttc cggattggtt taccatagtc cttttttttc
    23401 tgattgtggg cattacaaca gtaaaagttt tttatcagaa tattttcaga caattggtcg
    23461 aagctgttta tagttttgca gtaaccaatc aggtggtgcg cgatgaaaat ttgttagtgc
    23521 aacgtgcttc agttttgcta aatgtaattt tttattgtag ctcaggtttg tttttttatt
    23581 ggatcagtgt gcgctacaac tggaatcatc cgatcttaaa tgaaggattg tttcgttttt
    23641 tcttttttgc tttcgtgaca gcagtttttt ttacagtaaa aatgttgttg ctgaaactga
    23701 taggcacaat ttttaatctc gataaaccgg tggcagtttt tattttttcc atttttttga
    23761 ccaataattt actaggagtt ctgctggtgt tgattacttc atttatggca tatttatccg
    23821 gtgtgggtct gataaattac atctatctgg tattaggaat tctttctctg gcatttattt
    23881 ttttattgta cagggcattt ttgatttccc gtactgtgca gggttattct ttgtattatt
    23941 taattttata cttttgcacc cttgaaattg taccgattgc attgatagta aaatttgcat
    24001 cggagtaatt aaacaaggtg attaaaaacg catggccgga aattcgaaag taaagacaat
    24061 actcgtaaca caacccaagc cggaaacgga aaagaatcca tactttgacc ttgcaaaaaa
    24121 gttcaatgta aaaattgatt ttcgtccttt tattcagatt gaaggaatac ctgcaaaaga
    24181 ttttcgcaaa gacagagtta atgttgcaga atttcaggca gtaattttaa ccagtcgcca
    24241 ggctgccgat cattatttca gaatggctgc cgaaatgcgc atgacaaatt ttgatgaagt
    24301 aaaatttttc tgcgtgtctg agtcaactgc tttttatctc cagaaatata tcacatatcg
    24361 taagcgcaaa gttttttatg gcaacaacag cattaacgat ttgacagatg tgttgaagaa
    24421 acataagaaa gaaaagtttt tgcttccttg tagtgaagta atgaatcccg acatcattaa
    24481 cacattaaaa gacggtgacc tgaattttat aactgcagca atgtacagaa cggtaagtag
    24541 cgatttatct gatgtgaata ttgaaacgtt tgatatgatt gtgttcttca gcccatcggg
    24601 agttcagtcg ctgttgaaga attttcctaa atacaaacaa ggcaataccc gaattgcaac
    24661 attcggtgtc accacatcac agactgcaaa agatgctaag ttaaaagtag aggttgaagc
    24721 acctgttccg caggcaccaa gtatgacaat ggctattgag cagtatctga aaaaagccaa
    24781 caagtaattt tttacttaat ctcacttagt ttttgctgtg cctgtgtagc gtaaaatcct
    24841 ttgctggaaa ttattttttg taataactct tttgctttat cctgctcacc tttttgcaaa
    24901 agcagcaatg ctctgtgcca cgctgattcc tgattaaatg tattgtttga ctgtgcatct
    24961 aatcggtcga ggaagtgaag tgctttatca tactgctgac tttcgctgta tgacattgcc
    25021 gtataaaaca aggcatttac atcgctatga ttgtcggata gcaaaagttg caacttatta
    25081 attgcagagg agtatttctt attgttgtaa tcaactaatg cttcagctaa aaaatcttga
    25141 tatcctattg tacgagactg catgttgtta agcgttttgg atttttccat atcctttctc
    25201 gactcataac gggcaggaat gccgttgtct tcttttgcta cgttcttttc acgtttgtac
    25261 tcacgggaat agtcatacac ttttagtcct tcaatatttt ttgttaccgg cactacagca
    25321 tcaactacag cagcacctgc cgatgcagac tcagtaacta ttggtgcagc aacatctgct
    25381 gactgtggct cagcaattgc ttcatttttc tgaacaggtt ctgttgcagc aaaggcttta
    25441 tcttctttac ttgcttttgc taccattgca ttatgcgatg caattatgtt ttttgatgct
    25501 tgttgaattc tttcttctgc aggaggaggt ggtggttgaa tggtagattc attcaactta
    25561 gtatcactgc tggtttgagg tgtttctgta tttatttttt gttgtacagc aggtgcgtta
    25621 atttgttctg ctgtgttttc atgtttgaag gaatagttag aaataatcca tcctgtagca
    25681 attaaaattg caacagatgc tgcggcataa taccatctgt ttatagatgg ggattgtttt
    25741 gcagaagaag aatatttatc cgatacctgc ttgatggttt catcaagcgg tttcatagag
    25801 gtcaacaagg caagaccttc tgctgcatcg gaacaaagct cgcagtctgt caggtgtttt
    25861 tcaacggcat acttttcggc atccgtcatt tttccctgca catattttgt cagacgttct
    25921 ttgtcaacgc agtcagaggt tttgaatatt tcaattttac gacttaacat gattattttt
    25981 ctccatataa attctgagat tccttttccc attctgtata aaactcttca cctgcttgaa
    26041 atcaaaacct gttaggtctg ttatttcatt atagcatttt tctttcagga aaaacaattc
    26101 aatacatatt ctttgttctt cgtttaatgt ttttattgct tcttcaagca tcagcaattc
    26161 tgtttctttt tcataaacac ggtcggtgtt ttccggttca aattctgttg atgcagcaat
    26221 ttcatctcgg ctgtaattgt ctgctctgtt ttttgctgcc tgtctgctgc gcaactgcat
    26281 cagacaaaag ttttgtgcca cacgatgtat ccagtacgag aacttctgta cgtcatgttt
    26341 tgacaaatcg gcataaagtt tctcaaaaat ctgcaacaca gcatctttac tgtcgtcagt
    26401 atctttaaga tacttcatgc acacaccaaa tactaaatgc gaatagcggt taaacaatat
    26461 acccacagct tgtttatcac cttgctggcg atattgcaac acaagctcct catcgctaag
    26521 cgcattctta tccttattcc ggatgagcca cataaataat ttttactttc tgattaatgg
    26581 taggatgctt tctggaatat gattccataa acattcataa aaataatttt aaaattaaag
    26641 gactacctcc gcaatggcat gaataggtat atcttttcct gcctttaatt ccacatggtc
    26701 attggtgaca gcccaaacag ttgtttctac agcacaagtg ccttcagtgg tttcaaatac
    26761 tatgcgcact ttttgtttgt aatcgtttcc taatgccatt gcaccatcga gtaaaatttt
    26821 tcgccttgca cgatctattt cattcgataa cacttcgtgt tgaacaaaat ggtagtcaga
    26881 taaatcttct ttagcaatga tggtgagttc catattgatt gaatttggtg tatcagagtt
    26941 ttagcggcat ataaaagtag gcatttattc taatatgttc aataaaattt taaaacggcc
    27001 atttttcccg actttccggc catccagacc tttttttgtg aaggtgctat gctgatggtg
    27061 taaaatgcta gtgatagtcc atctgcgttt ttaatttctt taatgtagtg ctgactgtca
    27121 atcacatacg tgccgttatg gcctgttgta atcaggtagt tgccattttt agtttttatt
    27181 gttttaacac atgacacaaa agccccctca ggaagtttta tgggttgcca tgtcttgcca
    27241 ccattgtttg tttggtacaa agttgtggta gtatcggatt gcaaatagtt gcctccacca
    27301 atatagccat ttagtttgtt ttcaaaatac atagtaaaaa ttcctgtcat ggtgctgtcg
    27361 ctaatcatag gtgtggtttg tggaagaaat gcaccatcat tttcagaaat aaaaagtctt
    27421 gatgtgcttc ctcctgttgc aaaaatcacg ctgttttgca gccattgtat gttggtgtta
    27481 cttgcagcaa agcaaccttc accttttatt gcaggtgatg taacttttag ctcctgccag
    27541 ttgttgccgc catctgttgt tttaataatc ataaaatacc cttccattgg atcgcttata
    27601 gcaatgccat gaagcgaatc tttaaatgtc atactatcaa aaaaaatacc cttgctgtga
    27661 ttgctgtaaa catgttgcca tgatgtacca taattatttg ttctcaatag atatgccggt
    27721 gaatcaatgc tcaataacaa aaccgttgag tcgtttaatg ccgccaatga cctgaattca
    27781 gaatattttc catcagtggc aatagagtct atatgccagt tgctgccacc atcttttgta
    27841 aaaccaaaaa cacctctgtt agctgcaaac cacaccacac tgtcgtttac tgtctgtagt
    27901 gcgcgaatgc ttacctgaac gggcaattga atggttttta attctatctg ctgtgcctgc
    27961 agtgatgatg taaaacaaaa gcaccacagt atgtgaaaaa ttctttgcat tgtgtttcaa
    28021 atgtagtgtt ttttcaagtc tgcatagatt gttaaacgaa cagattgtaa tttttttaag
    28081 actaataggt agtgacttaa aaagaaagat atgttttcag aaaaaagtaa tgacagaaag
    28141 gtttatattg tagccatcag aatgtttgta ttataatcat gtcagcgcaa cttaaccgtg
    28201 tcatagcaca catggacctc gattgttttt ttgtatccgt atcgcggctg ctgcatccgg
    28261 agctaaacaa taagccggta attgttggcg gtggcgagcg tggtgtagtg gctgcatgca
    28321 gctacgaaac cagaaaattt ggtgtgcact ctgccatgcc cattaagcag gcaaaacgcc
    28381 tgtgcccgga agctattatt gttcgtggcg attatgatgc ttacagcagt cattccgata
    28441 tggtgcatga aattatttgc gaaagtgtgc cgctgtatga gcgtagcagt attgatgaat
    28501 tttatttaga cctaacaggc atggatcgtt tttttggatg ttataaacat gctacagaac
    28561 tcaggcaacg catcatccgc gaaacagggc tgcctatttc ttttggtatg agtgccaaca
    28621 aaaccgtaag caaaattgct accgatgagg caaagcccaa caatcagatg caggtggact
    28681 gtggcgaaga gaaaattttt ttagctccgc tgtcggtacg aaaaattcct atggtaggcg
    28741 aaaagaccta cacactgctg cgcagcatgg gtgtagagaa agtaaaaacc gttcaggaaa
    28801 tgcctgtaga actgatggag cgtgtgcttg gcgaaaacgg aactaccatc tggaaaaaag
    28861 ccaacggcat tgacaccaca gccgttgagc cataccatga gcagaagagc atgagcaccg
    28921 aagaaacttt tgagcaggac actattgacg tgcaacgcct gcagcacatc ctcattgcta
    28981 tgacagaaaa actatgcttc cgcctgcgca gcgaacacaa ggtaacgtca tgtataagcg
    29041 taaaaatccg ttacagcaac ttcgacacac ataccatgca gtgccgcata gcctacacaa
    29101 gttgcgacca cattattatt aagcaagtaa aagaactgtt tgctaagctg tttaaccgca
    29161 gaatgctcat caggcttatt ggtattaagt tcagccatct ggtaggtggc ggacatcaga
    29221 ttaatttgtt tgaagacagc gaagaaatca tcaacctcta tcaggcaatg gaccgtatgc
    29281 gcctgcgctt tggcgaagat aaaattcacc gtgctgctgc catgagcttt tcattgcgcg
    29341 gattcaatcc gtttaatgga ttgagtaatt cacctaaaga gataaagggc ggtaaataag
    29401 ctaaaaaaat agttgtagaa cgataatacc ttgaaaaggc aaaattccat atttactttt
    29461 acggtcttaa ttttaattat atatattcat aattaaactt aggtacctac tatggagttt
    29521 gataaaaaca tttctgatta aacaacacaa aacaatcaac ctttcaaaga tatcttactg
    29581 tatttgttcc tatattccag cggagtgatg cctgtatgtt ttttaaataa atctctgaat
    29641 gcttttgtgt cagtataacc cacatcaaac attacttcag taatattttt tctgctgctt
    29701 tcaaacttgc gttttgcagt ttcaattttt actcgttgaa tatattccaa taccgaatga
    29761 ttggttgact tcagaaatct tcgctcaaaa gttcttcggc tcatctttgt atagtctgca
    29821 agttggtcta tagttagttt tgcatcagtg tgtttttcta tatactgttg tgcttttaaa
    29881 atttctgtgt catgatgatt tttctgacca ttgaatatgg caaaggaagc ctgactgttt
    29941 cgatccaaat caatagcaaa gtattttgaa gcaagaatag cagtgtgtcg gtctgaatat
    30001 ttttccaaca aataaaacag cagattccac agcgagtttg caccaccgct gctgtaaatg
    30061 ccacgctctt cggtaatgat tgcgccatca actacctctg tatttgcaaa ctgttcttta
    30121 aactcattaa aataagccca atgtgttgac acttttttgc cgtcaaccaa acctgatgct
    30181 gttagtagaa aagcaccaat acataaactt gcaacttccg taccctgacg atgcattttt
    30241 tgtagccatg gaatggcagc tgcatttttc tttaaggaat gttttacatc accataaagc
    30301 gcgggtataa ttattaagtc agaatcgtta acactattta tatgcctatc aatacgaatg
    30361 ctgtattcgc catcattggc tttgatgctt ttttgtaagc ccacatattc aactttaaac
    30421 ttaggttttt tacctgaggc tataagaata tcattggcag ttttaaataa tctgtatgcc
    30481 ggtgttatgg cttctatagc tgcatgatac ggtacataaa cagaaattaa cattccggta
    30541 atttttattt ggtaaagtta aagtattttg tcattatcac accttaaaac tgtcgtttct
    30601 gccacctatt gttgctttct ttaatactca tctttgtagt actaaatcaa ttattcacta
    30661 ttaagcaaaa aattatgcaa acaaataatg taaatctaca cagggtattt gcttcgccta
    30721 cagagaaagt gtttaaagca ttttctgatg ccgatgctta tactgcatgg ctacctcctt
    30781 atggatttgt ggccaaaaca catcaatatg attttaaagt tggtggcagt tataaaatat
    30841 cgtttattaa ttttacaaca ggctttagtc actctttcgg tggtgagttt gtagagataa
    30901 agcttaatga cctgataaaa ataacggatc gctttgatga ccctaatctt cccggagaaa
    30961 tgataactac tgtccatttt aaaaaagaga gctgtggtac agaaattaat atttcgcaaa
    31021 caggaattcc tgttgccatt ccttcagaga tgtgttattt gggttggcaa gattcattag
    31081 ataaattaaa aagattggtc gaacccaata taccggatgc ttagtttatt cgctattaaa
    31141 tttaaacaaa cataaatcaa tataataaca attttcaaaa cactatcatt atgtcaaaac
    31201 aagtttttat taatcttccc gtaaaggatt taaagaggtc aatgtctttt tatgaagcta
    31261 taggcttcac caacaatcct cagtttacag atcatactgc tgcctgtatg gtttggagtg
    31321 acacaatttt tgtcatgctt ctaactcatg ataagtttaa gcagtttaca tccaaactaa
    31381 ttattgatgc aaaacagtca gtaggcgttt ctaatgcact tttttttgaa agtattgatg
    31441 ctatgaagtc gtttgcagaa aatgcagtaa aggccggagg cagagaatat gcagctgcca
    31501 gcgactatgg ttttatgcaa caacatttta ttgaagattt agatggacac aattgggagc
    31561 cattctatat ggatatgagt aaatttcccg gataaataac aaacaaatta aaattatggc
    31621 aaagaaaaaa atagctccat gcttatggat tgaaaaagat gctaaaaagg cagctaaata
    31681 ttatctctca atttttaaag aaggcaaaat gctttcgtat aaagcatata aaaactcagg
    31741 cacacccagt ggcgattttg atattgcata catcaacttt ctgggaatgg atgtgcagat
    31801 tcttgctgca ggaccattat tcaaatttaa tgaagcagta tcttttgtta tcatctgtaa
    31861 agaccaaaaa gaaattgact attattggaa ggcattgaca aagaatggtg gcagcgaagg
    31921 tccgtgcgga tggtgtaaag ataagtatgg tctgtcatgg caagttgctc ccgagctata
    31981 ttttaaatta gaacatcata aaaataaagc aaagcaggaa tatgctttca aagcattgat
    32041 gaaaatgaaa aaaattatca ttgccgactt agtagaaaaa aagtaattgt tggcaaaacg
    32101 ataaaaaaca aagaagatga ataaaattac tgtgagtgct attataaatg caccaataaa
    32161 aaaagtgtgg gactgttata caaaccccaa tcatattgtt aactggaatt ttgctgaccc
    32221 tagctggcat tgcccttctg ctactaacga tatgcgtatt ggcggaacct acaaagcaag
    32281 aatggaagca aaggatggca gctttggttt cgactttgaa gccgtttata ctcagattga
    32341 atccgagaaa tatttttctt atgagtttgg aggaagagtt gcttcagttg atttcaagaa
    32401 taacgatagc caaacagaag ttgttgttgt gtttgatgcc gaaagtgaaa accttgttga
    32461 gatgcaaaga gcaggatggc aggctattct gaataatttt aaaaattaca ccgagcataa
    32521 ttagtatatg caattatgtg cgtatctcca ttttaatggt gattgcagaa aagcaatgac
    32581 attttataaa aagtgcttag gtggaagact gagttttcaa atggttagcg aattgccgca
    32641 gtcagaatca cttcctactt atataaaaag gaacattctg actgctttat tacagactga
    32701 atattttcag ttagccggaa cagacctggg taatgaagca gtttattcgc ctgacaagag
    32761 cttttcgtta ctcttagaat gcaggagcaa accggagttg aagcgattgt ataagcaact
    32821 ttctgcaggt ggagtgaaga cacaattgct tcagaagaat ttttatggtg taataattgg
    32881 ctcttttaaa gataaatttg gcattaactg ggtccttcag tattaattgc actatttttt
    32941 aaatttattt tctttcagaa gttttgttac agtttttctt aatgtttata ttacaaccga
    33001 ccgaatgatt gtagtataaa catattaaat gtgtagagtg acagctattt ggttgtttta
    33061 acatattgcc atgcacctct gttgccactc atacttctct ctaaaatacg gtgtccttag
    33121 tccggacgat ttattgtgca ttgccgcaca aaaaaatatt cagtcgctgg cactcacaga
    33181 tatcaacaac acatcaggca tacttgattt ttttcgttta gcacagcgat acaatgttcg
    33241 ccctgtggca ggcattgatt tcagaaatgg tgtagaacaa aaatttattg gtattgcaaa
    33301 aagcaacgaa ggctttcatg agctcaataa atttctatcg gagcatttaa tttccggaga
    33361 aaaattttca gacattgctc ctgcattcca acaggcatgg atagtttatc caatgttgtc
    33421 ggcaccacaa cagttgcacg aaaacgaatt tgtaggcatc aaacccaatg agctctttca
    33481 gttaaacttt tcggagtgga aaagcaagtt acataaattg gttgctcttg caccggcaac
    33541 ttttcgcaac aaaacagatt ttaattcaca ccgtctgctg cgtgccatca gctacaatac
    33601 gctactcagc aagctgcccg tttccggaca ggcatcggca gatgaaatca tgtattcacc
    33661 ggaggagttg aagtgcattt acagagaata tccacaactg atttacaaca cagagaaatt
    33721 attggaaccc tgttgtatag attttgaatt cggaaaaaac aaaaacaaaa aatatttttt
    33781 gggtagtgcc aaacgcgatc atcacaaact gatgagtctt tgttatgaag gtttaagtta
    33841 tcgttacccg aatactgacg acaccatcat tgaccgtttt gaaaaagaaa ttcagatgat
    33901 aggtgagttg ggtttctcat cctattttct catcaatcac gatattgttc gctatgcacg
    33961 aagcaagaat tatttttatg taggccgtgg cagcggtgcc aacagcatgg tagcctatct
    34021 gctgcgcatt accgatgttg accctataga cctcgacttg tattttgaac gatttatcaa
    34081 tccgcagcgc accagccctc ccgattttga tattgatttc agttggaacg agcgcgatga
    34141 tgtgattgac tacatcttta accgcagcac ctttggtaaa gaacatgtgg cactgctggc
    34201 aacgtatagc acatttcaaa gagatgctgc cattcgcgag ttgggaaaag tttttggatt
    34261 gcccaaggca gagatagatt ctttttttga aaatggaaat accaaacgcg accacatcag
    34321 taatttggtg ttgcaatatg cacaacgtat cattgatgtg ccttcgcacc tgagcattca
    34381 tgcaggaggc attctcattt ccgaaaaacc catgacgtat tatacggctc tcgataatcc
    34441 gcctaaaggt tttccgctta cgcagttcag tatgctcgaa gcagaagatg taggtcttta
    34501 taagtttgat attttgtcgc agcgcggact gggaaaaatt aaagatgctg ttgctgtcat
    34561 tgaaaaaaac cgacaggtaa aagttgatat tcatgacata aaaaagttta aagaagatga
    34621 gggcgtcagg caaaaactgc agcaggcaaa tcttatggga tgcttttatg tagaaagtcc
    34681 tgccatgcgc atgttactga aaaaactacg tgcaaaaacc tatctcgatt tggtggcagc
    34741 aagttcaatc atccgtccgg gggtggcaca aagcggcatg atgcgcgaat atattttgcg
    34801 ctttcatgag gagagccgca gaacctacat tcatccgatg atggaggagt tgatgcgcga
    34861 aacttttggg gtgatggtat atcaggaaga tgtgattaag gtagcacatt attttgccgg
    34921 acttacactc acagaagccg acatgctgcg cagaggcatg agtggaaaat atcggggacg
    34981 tgcagagttt gaaaaagtga aagatgcttt tttcaatggt tgccgcaaga aaggacatgc
    35041 cgataagata gcagaagaag tgtggcggca gattgaatcg tttgcaggct atgctttcag
    35101 caaaggccat tcggcatcgt atgccgttga gagctatcag tgcatgtttt taaagaccta
    35161 ttttccactt gaatatatgg tggcatgtgt aaacaacggt ggtgggtttt acgcagctga
    35221 attttatgta catgaagtgc gtatgtgtgg aggcaccata catgcacctg acgttaataa
    35281 gagtttttat gaaacatcgc tctatgggaa aaatatttat gcaggctttc atttagtgca
    35341 tgaacttgaa atgttgacgg taacaaatat tttaaaagcc agagcagcag aagggcagtt
    35401 tatttcgctt gcagatttta tgcagcgcgt tgaaatttct ctcgaacaat tgcggctgct
    35461 cattcgtgtt aatgctttgc gttttacagg aagaaccaag caacaactgc tgtgggatat
    35521 tcatgaaata atggggcatg ataaaaaaac aaatacagaa aaacagttgt ttgaagccga
    35581 acacaaacat tttgtccttc cggaattgca ttacagcaaa attgatgatg tgtgggacga
    35641 aatagaattg ttgggatttc cgctgtgctc accatttgat ttgataaaac aaaaaacgaa
    35701 tgtaacggaa ggaatagtga ttcatactgc tgcagaactg tcggcatact gcggaaaaat
    35761 aatttccatt gccggttatt ttgttacccg caagccaaca cgaaccaagc atgggcagcc
    35821 tatggcattc ggtacttttc tcgacagcaa aggacaatgg atagacacta cacattttcc
    35881 cgatgtggta aaacaatatc cttttcgcgg acgcggatgc taccgcatta ccggaaaggt
    35941 agtgcaggaa tttggttttt acagcattga tgtcatcagc ctttatctgc ttgaatataa
    36001 aaccagatat gctgtggtgg aggaggataa agttcatgta aatgaatagg agtaaacaat
    36061 aaaaaaagta aaacttccct aagtaggcgc aagcgtcttt cgctcgtgct tttatccttg
    36121 tggcctctat ccgtccgata gctatcggac ttcatagttc aaccagcttt aaatcggtaa
    36181 attgattagc actactatac tccatacccg cgaagttgca cttcgtgggt ttttgtgcaa
    36241 agaagtttta cttctgaaa
//
LOCUS       DAOOOL010000009        35781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_85_length_35781_cov_152.134356, whole genome shotgun sequence.
ACCESSION   DAOOOL010000009 DAOOOL010000000
VERSION     DAOOOL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 35781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35781
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85_length_35781_cov_152.134356"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="rpsB"
                     /locus_tag="PKU82_01635"
     CDS             <1..832
                     /gene="rpsB"
                     /locus_tag="PKU82_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOZ81603.1"
                     /translation="HFGHLKRKWNPKMAPYIFMERNGIHIIDLNKTAAKLEEATAAIK
                     QIAKSGKKILFVATKKQAKEIVANAAKSVGMPYITERWPGGMLTNFATQRKSVKKMTG
                     FDRFMKDESFNNISKKERLQVSRQKAKMEKLIGSIADLTRLPAALFIVDVLKEHIAVS
                     EAQKLGIPTIAICDTNTDPTLIDFPVPANDDATKSISLIMNAMIKAIEEGLEERKMDK
                     EADREREAAAEEEGGERGEGEKEFTMSDEGDDSGETKTKSPAKKATTRPRKGTTTSKR
                     "
     gene            875..1708
                     /gene="tsf"
                     /locus_tag="PKU82_01640"
     CDS             875..1708
                     /gene="tsf"
                     /locus_tag="PKU82_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOZ81604.1"
                     /translation="MSTVTISASDVNKLRQMTGAGMMDCKKALMEANGDFEKAIDELR
                     KKGQKVAANRADREAKEGYIVAKTNNNATKGYLVALSCETDFVAKNAEFVKFAEDVLE
                     TALTNNAASLDALLALQLQGTTISEKLIEMVGKIGEKIEITRYEVIEAPKVVVYIHPG
                     NRVVSMVGLSSATAANEAGKDVAMQIAAMNPVAIDKDDVDQSTLDREIEIGKEQARAE
                     GKPENMIEKIAMGKLNKFYQESTLLNQAFIKEDKKTIRQYLDGVEKGLTVKAFRRVQI
                     G"
     gene            2001..3725
                     /gene="lysS"
                     /locus_tag="PKU82_01645"
     CDS             2001..3725
                     /gene="lysS"
                     /locus_tag="PKU82_01645"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOZ81605.1"
                     /translation="MALSEQEQIRREKLNELLQMGINPYPAEKFEINTNSTFILENYE
                     QAEARFGAGKEEVSIGGRIMSVRDMGKAAFAQIQDAEGKIQIYIKRDEICKGDDKTLY
                     DTVWKKLIDIGDIIGIKGYVFKTKTGETSIHASSFILLCKSLRPLPIVKEKDGEVFDA
                     VIDPDLRYRQRYVDLIVNPQVKETFRKRTRLVNTMRNYLNEKGYLEVETPILQPLYGG
                     AAARPFKTHHNTLDMTLYLRIANELYLKRLIVGGYDGVYEFSKDFRNEGMSRFHNPEF
                     TQIELYVAYKDYEWMMNLVEEMIEKVALELHGSTAVKVGEKTIDFKRPWKRFTMYEAI
                     EHFTGVDVSEMDEMAMRKAAAQMGIELDDTMARGKIIDEIFGSKCEANLIQPTFITDY
                     PVEMSPLAKKHRSKPGLVERFEAICNGKEMCNAFSELNDPIDQRKRFEEQLELGKRGD
                     EEAMQLDEDFLRAIEYGMPPTAGLGIGIDRLSMIMTNAASIQDVLFFPQMKPETIETN
                     AATDTGLSHIPAPWNDVISRMGITSKQQLKEANPNKLFNDMGGMRKKLKLDISMVTLE
                     EVKSWINV"
     gene            3809..4336
                     /locus_tag="PKU82_01650"
     CDS             3809..4336
                     /locus_tag="PKU82_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ81606.1"
                     /translation="MATKRIDLKAFRIKSGRNRKKYTTFLKGLHSRKVKQLTAKAKKL
                     NDLAFKKIDCLACGNCCKTMTPTWQPADIKRVAAHFKMTVQQFKDKWLYKDETGDWMN
                     TKTPCQFLQRDNKCSIYEIRPKDCSGFPHLQHTDFLIGREVNIPNMTSCPITYEVVER
                     MYKLVVEKDATVLND"
     gene            complement(4345..7242)
                     /locus_tag="PKU82_01655"
     CDS             complement(4345..7242)
                     /locus_tag="PKU82_01655"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOZ81607.1"
                     /translation="MSFFKTALLSCWLLCNSILYAQPTYTQYVNPFIGTGGHGHTFPG
                     ATLPFGMVQLSPDTRIDGSWDGCSGYHYSDSVLYGFSHTHLSGTGVSDYGDILLLPMN
                     DEPALDESVPTATFSHNFENATPGYYSVKLLADSIDVELTSTLRTGMHRYTFNKPGLA
                     YIYLDLNHRDKLTASNINIVYDSTIKVYRKSEGWAKEQILYANIRFSKPFIQSKQLGN
                     KKAVFVFSVSEKESIMAKVALSAVDDDGAERNMILENPDWNFEHIKINAAQKWNEELS
                     KIKVSSSDKNKLTVFYTAMYHCMMHPNIYNDVDGRYRGRDLQIHQTDHNYYTVFSLWD
                     TFRTWHPLMTIIDSARTVDFIRTFLLQYEQGGLLPVWELAANETECMIGYHAVSVIAD
                     AMMKGIKGFDYEKAFEACKFSAEQRNRFGLGAYIDKGFLEAEDEHESVSKTLEYAYDD
                     WCIAQMAKLLGKEDDYNTYMQRSQSWKNLFDPVTGFIRPRSNGGWLNPFDPFEVNNNY
                     TEANGWQYNFFVPQDIDGMISYNGGKEKFEKKLDQLFSASTKTTGREQSDITGLIGQY
                     AHGNEPSHHIAYLYNYVDKPGKTEKIVNKIINDFYKNSPDGLIGNEDCGQMSAWYVMS
                     ALGMYPVCPGSNQYTLTTPQFDKVEILTSGNKTFTIDVKNKTNKANFLRVKKTKNISF
                     RDGLLFNHNRFSTDNLLVLTADSIPSILRKKDLADSIYYSDDLFRVPIITASTTVFKD
                     SLKVSIKSFQKDKNIVYLQQGPFERPAKIDYKKPFYIYDTSVIHAFVTDSSGIESKWA
                     KAEYFKLPHPKWKVSIKGNYNPQYNAGGANGLIDGLHGDINWRKGRWQGYQSQDFECV
                     VDMGNQQEVSSFNTSFLQDTRAWILMPISVEYFTSDDGKLFNSAGIVQHQTAPDNYEI
                     SIENFKLQTEALITARYIKVVAKNFRKLPSWHQGFPMGGEAFIFVDEVEVE"
     gene            7576..8934
                     /locus_tag="PKU82_01660"
     CDS             7576..8934
                     /locus_tag="PKU82_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81608.1"
                     /translation="MKRTIQSLIFIAVTLLTTNIFAQSWQLVGPVGFTSFNAKYTNIA
                     FNNNDEPYVVFSDGSVNAKASVMKFDGSNWVYVGTAGFSQGIADYTDIAFDNNGTPYV
                     AYSDHANNDNVTVMKFDGSSWVAVGNVGFSTGEPYYNSLAINSNGVPYVVFQDATSLS
                     KATVMKFDGNSWVVVGTAGFSSVSVKEPTIAIDNNNTPYVAFTQWGGLYNASVMKFDG
                     SNWVNVGPVGFTTNSADYASLAFSNSGEPYMAFIDGTQNSHVSVMKFNGSSWALVGNA
                     GFSTGTSHYPSLAFSNTGEPYVAFKSSFNNSQATVMKYDGSAWVAVGSTGFSSGWADY
                     TSLAFNSSGVPYVAFEDINNSYRATVMKFGNGTSSINSMAADNSIEVYPNPANDYLNL
                     TNLPVGANIKINDAIGKNVYDASITTKETSINTSAFTNGIYHICIEKDGVLINKKLVI
                     NK"
     gene            complement(9071..9661)
                     /locus_tag="PKU82_01665"
     CDS             complement(9071..9661)
                     /locus_tag="PKU82_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ81609.1"
                     /translation="MKKYYVLYKPYGYLSQFSDEAKHKGLKHLIDVEPDVYPAGRLDV
                     DSEGLLLLTNDNFLKHHLLNPENKHKRTYHVQVDGTATSVHLNLLRQGVEITIEGKPY
                     KTFPPASVNLLNEVSYPERNPPVRFRKHIPTSWIELVLTEGKNRQVRKMTAAVGIPTL
                     RLIRSKIEAINIKDYQPGELKELSQKEIFKLLHLQI"
     gene            9807..10388
                     /locus_tag="PKU82_01670"
     CDS             9807..10388
                     /locus_tag="PKU82_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ81610.1"
                     /translation="MVDVQELKNILCRYYNSVSDKTIDKIAQHFEYRNYKKGDTVVSN
                     KSICTQCFFICSGYTRGYIYREDAQFTLWFGEPGDMITSFSTMFQNEKGNEIIETLTD
                     CTMLAINMQTFKKLISEEIEIANVYHKIVEAGYLFWERRFLIYSQERAEDRYKEWYNR
                     SKHISEHISLGVIAQYLNIDQATLSRIRSKIKK"
     gene            complement(10429..11562)
                     /locus_tag="PKU82_01675"
     CDS             complement(10429..11562)
                     /locus_tag="PKU82_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81611.1"
                     /translation="MKNRTILFAFFLLLVSIGYSQTCTVGYPPPSDNCATAPAIQLNG
                     VYCGNTCNATANDAAWFKNSNEFCNSVSKQTIENNGFYIFQATASSVNFTICTKTGCT
                     RKNNIIGASGIQALIFNFPTANGLGTCGSGSIDGYYCLKQLSGTDCGSCSNPKGCLSA
                     SVNGLSAGKFYYFMVDGYEGDCCDFSIQFTSNITLPVELISFKGKGENAGNHLFWSTA
                     SEKNNDYFNIESSTDATNWRTINSIKGHGNTNQLKNYDFIDLNPAAVITYYRLMQVDY
                     DGSFSYSPIIFVDRSATGSLSIFPNPFNSELNLQFNISKGGLYTLTFNSMLGKQIQKQ
                     IDLKEGQSLAKIDFSDNLTSGAYMVEVANESGEILQHVKLIKY"
     regulatory      11865..11970
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12053..12715
                     /gene="mnmD"
                     /locus_tag="PKU82_01680"
     CDS             12053..12715
                     /gene="mnmD"
                     /locus_tag="PKU82_01680"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOZ81612.1"
                     /translation="MDSIEIVKTKEGIDTLINTRINEHYHSLHGAMQESMHVFIRNGL
                     LALNKSLPDIKILEMGFGTGLNAILTYRECYVLQKPIHYTTIESSPLPMAIINKLNYF
                     EYFGKALLPMFQKMHDCNWFENVTFGDFVLHKINADMLELKLDGGYDLVYYDAFSPTH
                     QPELWSFEVLHKVYESCNRGAMLVTYCANGEVKRTLKKVGFAVEALPGPKGKREMIRA
                     CK"
     gene            12754..16416
                     /gene="metH"
                     /locus_tag="PKU82_01685"
     CDS             12754..16416
                     /gene="metH"
                     /locus_tag="PKU82_01685"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOZ81613.1"
                     /translation="MKNIRQELEKRILIIDGAMGTMIQRYKLSEEDYRGERFADWKSD
                     LKGNNDLLSLTKPEIIEAIHLEYLEAGADIIETNTFNAQKISLADYHMESLAYELNFE
                     SAKIAKKAVESFKKKHQSDKKFYVAGAVGPTNRTLSLSPDVNRPGFRAVSWDQMVDAY
                     TEQINGLVDGGADLLLVETIFDTLNAKAALVAIQNVMEQKKVDLPVMISGTITDASGR
                     TLSGQTVEAFLNSVSHVNLLSIGLNCALGAKEMRPYLEELSAKSPFYISCYPNAGLPN
                     QFGEYDETPHQMCVQIDDFLESGFVNIIGGCCGTTPEHIKHIAEHAKNHKPRAIPTIE
                     PYLRLSGLEAVTLRPDSNFMNVGERTNVTGSKKFLRLIKDNKYDEALSVAREQVEGGA
                     QAIDINMDEGMLDAVEAMTTFLNLIAAEPDISKVPVMIDSSKFEVLEAGLKCIQGKGI
                     VNSISLKGGEDQFIYEAKTVKKYGAAVIVMLFDENGQADSTPRRIEIAKRAYDILVNK
                     VNFPAQDVIFDPNIFPVATGLEEHRQNAISYFEASKWIKENLPLAKISGGVSNVSFSF
                     RGNDPIREAIHAVFLYYGIKNGMDMGIVNPSQLIVYDEIEKDLLALVEDVILDRREDA
                     TEKLLAKAEELKLSQTTTDKNVKKGNQSANAWRSEDLQSRITHSLIKGIDEFVDQDME
                     EARQQVSKALEVIEGHLMIGMGVVGDLFGQGKMFLPQVVRSARVMKKAVAYLTPFIEA
                     EKVKGQSKGKIVLATVKGDVHDIGKNIVGVVLACNNYEIVDLGVMVAADKILQAARDH
                     QADIIGVSGLITPSLDEMVHVAKEMEREKFTMPLLIGGATTSKVHTAVKIEPNYSGVV
                     VHVNDASRSVPVVSNLLSAEQKEKFIEDIKAENERTRSYHKAERSRGKFLSLKEAQQN
                     KYELNGAVVTEPKFKGVKVFSNYDLSLLTEYIDWTPFFIAWEMKGSYPKILSDPQRGQ
                     EAQKLFDDAQTMLQRIIKEKWLTANAVFGIFPAANKGDDIEVFDERRENKIATLHTIR
                     QQSERATGQKNIALADFIRPSDYIGAFAVSTGFGIEKWLKKFADDHDDYSSIMLKVLA
                     DRLAEAFAEHLHERVRKEFWAYTTDENLNRQDLIKEKYQGIRPAAGYPAQPDHTEKYT
                     LWKLLDVEKNTGITLTEHLAMFPTAAVSGLYFAHPESHYFAVGKIQRDQVEDYAKRKS
                     MTVEEAEQWLGSILGY"
     gene            16508..17686
                     /locus_tag="PKU82_01690"
     CDS             16508..17686
                     /locus_tag="PKU82_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ81614.1"
                     /translation="MSKDRFQAPDYFLMDELLTDEHKLIRDSVREWVKKEVSPIIEDA
                     CQKCEFPKHLIKGLAEVGAFGPYIPAEYGGGGLDQISYGLIMQELERGDSGIRSTASV
                     QSSLVMYPIYTYGSEAQRKKYLPKLASGEWMGCFGLTEPDHGSNPGGMVTNIKDKGDH
                     YLLNGAKMWISNAPFAQVAVVWAKDEEGVIRGLIVERGMKGFTTPETHGKWSLRASAT
                     GELVFDNVEVPKENLLPNVKGLKGPLGCLNSARYGIAWGALGAAMDCYDSALRYAKER
                     KQFGRPIGGFQLQQKKLAEMLTEITKGQLLVWRLGKLKNENRANPAQISMAKRNNVHT
                     ALWCAREARQIHGGMGITGEYPIMRHMMNLESVITYEGTHDIHLLITGMDITGENAFT
                     "
     gene            complement(17701..18429)
                     /locus_tag="PKU82_01695"
     CDS             complement(17701..18429)
                     /locus_tag="PKU82_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HOZ81615.1"
                     /translation="MKRYLFILITLFSSVALAQQSGNGFAVVELFTSQGCGSCPAAET
                     VLKKEMDDAIKNNKPVYFLSFHVDYWNKYGWKDPYSSIKYSRRQNNYVSATGSNEVFT
                     PQVFVNGNTGFVGSDSKRLNSEIEKGLKSVAKQKLTVTVLQTLNDTLHLNYTASKADA
                     NANIIVAVVQKSVTTKITKGENIGRTLVQDNVVRLYEVYPVATKGTLLLKTNKLALNE
                     NFQLIAFIQNKQSKKISAATALTL"
     gene            complement(18518..19114)
                     /locus_tag="PKU82_01700"
     CDS             complement(18518..19114)
                     /locus_tag="PKU82_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOZ81616.1"
                     /translation="MEYIKHLSKDKKLASVIKQHGTVELKKRKDLFLSLCGSIISQQL
                     SVKVADVIKQRFYDLFDGKPTPEKILQATTETLRSIGLSNSKASYIKNIAAFALENSL
                     QLNKVNKMSNDEFIEYITQIKGVGRWTAEMIMMFTLGREDVFSVDDYGIQQSVIGLYK
                     IKISDKKKLRLRILAIAEQWAPYRTYACMYLWRWRDNG"
     gene            19709..20344
                     /locus_tag="PKU82_01705"
     CDS             19709..20344
                     /locus_tag="PKU82_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ81617.1"
                     /translation="MQIKKFTFNPFEENTYIVYDETAQCAIIDPGCYMPEEKEELLSF
                     IAEQKLNPVLLLNTHCHVDHIFGNKLIYDTFGLKPQFHQLELRLLEAAGEYSKQWGIF
                     LEPSPTAEKYLEEGDIISFGNISLEILLTPGHSPGSLCFYDKQEKIIIGGDVLFQMSI
                     GRYDLPGGDEQTLLNSIRTKLFTLPADVKVLPGHGPETTIGFEKMYNPFLV"
     gene            complement(20476..21114)
                     /locus_tag="PKU82_01710"
     CDS             complement(20476..21114)
                     /locus_tag="PKU82_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81618.1"
                     /translation="MKKLTFLLLFIAGNMFAQSNSKFEKTMVKQIDLLDKAKAGTDFV
                     NAANAFERIANAETKEWLPLYYQAYALVLSSNDEKDNVKKDAVLDKALEIANKADKLS
                     PDNSEIVTLQSWITSMKIGIDPATRGQKLGMEAGMLTAHAIELNPENPRPYFLQGTGL
                     LFTPEQFGGGKAKALPVLEKAIEKFNAFKPANAMMPNWGKEFAQKMLDECKK"
     gene            complement(21138..23294)
                     /locus_tag="PKU82_01715"
     CDS             complement(21138..23294)
                     /locus_tag="PKU82_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81619.1"
                     /translation="MYLPKHVISWLFFLIIIQSATAQTTISGTVTDQKNNPVAGANIV
                     IRDSYDGASSDASGKYQFNANDSGDVIIVCSLIGFSPSDKKIKLQGNTLEINFKLKET
                     SNELNTVTITAGAFEASDEKKMVILRPLDIVTTAGSQGDIYGALQTLPGTQPTQDQEG
                     LFVRGGDAAEAKTLIDGIEVANPYFSSVPDVPQRSRFSPFMFKGTNFSTGGYSAQYGQ
                     AMSSVLALESEDLPPRSVGNIGIMSVGGGGGYMHRWKKTSAGAFANYFNLAPYFAIIK
                     QNRDWDSPPQTFSSSAVFRHKTSETGIIKAFINYQYSDLSLNFDNLEDVTGKSKNHFG
                     LNNYNIFSTLSYKEVLNKWTVQAAGSYSKDINRIYARPQHLLFSNQLWQGRAVVSRSI
                     SELSSIRGGVEVKYNDFSNLYNNFRVDSTEMFTAVFLEGDAYITRKLVARAGLRVENS
                     YLLRASNLAPRISVAYKLGKFDQLNIAYGDYYQTPDKNYLIFTNLDHFEKATHYILNY
                     QYVNEKRTFRVEAYYKKYNHLVQTTNMASDVYATAFATGDDGKGYAQGIDFFWRDKQT
                     FKYVDYWISYSFLDTKRKYLYYPFEVQPTFASKHVVSVVMKRWFPKLRSSIGFTYVYA
                     AGRPYYNPNKTNENNFLSDRTNDYNNLSLTWSYLTSIGRHFTVISAAVNNVLGIESAY
                     SYRYSTDGLRRSAVGPTSKRSFFIGVFISFGEDRTE"
     gene            complement(23349..23933)
                     /locus_tag="PKU82_01720"
     CDS             complement(23349..23933)
                     /locus_tag="PKU82_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOZ81620.1"
                     /translation="MLKVDDILVSDQVITEKFICDLSKCKGACCVEGESGAPLEEDEL
                     KALEKVYPKVKPYLTDEGIKAIEAQGFHLKDDDDEWVTPLMWPGGPCAYTVFENGIAL
                     CGIEKAYRDKKIKFYKPISCHLYPIRVLKTKSGDALNYHEWEVCSPACKLGKKEKVRV
                     YEFLKTALTRKYGEAWYNKLIKAAQEYLQTLTKK"
     gene            24008..24763
                     /locus_tag="PKU82_01725"
     CDS             24008..24763
                     /locus_tag="PKU82_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ81621.1"
                     /translation="MNPIITVNNLVKHYDSFHAVKGISFEVYEGEIFGLLGPNGAGKT
                     TTLEIMETLRAKTSGDITIKGNKVGTDDDAIKKIIGVQLQAAGYYPSLSLLELIDLFA
                     GLYSQDVNAMELLQKVGLTDKAKSKYKELSGGQKQRFSIATTLINKPAVIFLDEPTTG
                     LDPQARRNLWDLIRQIKNEGTTVVITTHYMDEAEELCSRVAIIENGKIIALDTPDQLI
                     DDLVAKGFERKKEVKKANLEDVFLNLTGQEWRE"
     gene            complement(24774..25391)
                     /locus_tag="PKU82_01730"
     CDS             complement(24774..25391)
                     /locus_tag="PKU82_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81622.1"
                     /translation="MLKSGLALCLLFILSLGNINAKETIFKEQRIGFGYYPSFHGSQN
                     FWLNYSVNKRSRKTIYSIRGMYNFRSEKAQEVSNYAIGPCVSVKLLSFFKERLEIRPT
                     LYIPYVNFKYEKSTYPGSLYATQYNGAALLPGLNIQTELTDRIGVRYGANAGIYYAKT
                     REPIFEQKNGVTTYDVYTDKNMYWIMLIDFSITIKITKNYLPHQR"
     gene            25490..25930
                     /gene="rnpA"
                     /locus_tag="PKU82_01735"
     CDS             25490..25930
                     /gene="rnpA"
                     /locus_tag="PKU82_01735"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOZ81623.1"
                     /translation="MNEGTLRNTFSKAERLCKRKDFELIFSQGAAFNRFPFRVHWLKL
                     KENTSTVPVKIAISVPKRKIRSAVSRNRIKRLIRESYRLNKQILSNELKKNEQVIHIM
                     FVYNGNINATFTEIQSKIILILQRLKELNDQTAEQDSDSHSKVL"
     gene            25881..26102
                     /gene="yidD"
                     /locus_tag="PKU82_01740"
     CDS             25881..26102
                     /gene="yidD"
                     /locus_tag="PKU82_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOZ81624.1"
                     /translation="MIKLLNRILIAIVKFYKMVISPLLPMSCRYYPTCSEFCMEALKK
                     YGPLKGGFLSLKRILSCNPFGGHGHHPVP"
     gene            26154..27830
                     /locus_tag="PKU82_01745"
     CDS             26154..27830
                     /locus_tag="PKU82_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOZ81625.1"
                     /translation="MRKRIRLYVAVILVGISAFSVYSFSDDYFEISRNLDIFATLFRE
                     LNIYYVDETNPGDLMKKGIDDMLASLDPYTNYIPESEIEDYRFMTTGQYGGIGSLIGS
                     RNNEVIITDPYEGFPAQKADLRAGDVIVELDGKPIKGKKNDEVSKLLKGQPKTSVKIT
                     IRREGEPNLIDKNVVREEIKINSVSYSGIIDNDLGYIRLTGFTENAGQEVKNALQALE
                     AKTKLKGLIFDLRGNPGGLLNEAVNIVNIFVNKGVEVVSTKGKAKEWDKTYHALNNPV
                     DTEIPVAVLVNSSSASASEIVSGSLQDLDRGIIIGQRTFGKGLVQTTRPLSYGAQLKV
                     TTAKYYIPSGRCIQALDYSHRNDDGSVGKIPDSLITQFKTKSGRTVYDGGGVMPDFVT
                     DVRQLSPISQSLLLKYLLFDYATMYRNTHPSIAPAKEFHLSDAEYAEFVKWISAKDYD
                     YETKSEKILDDFKTTAEKEKYFSAAAAEFEVLKKKISHDKNADLQKFKDEITELLENE
                     IVSRYYYQSGQIEASFKNDLEIKKATEALKNKDVYSSIMKSSMASVREKH"
     gene            27843..29414
                     /locus_tag="PKU82_01750"
     CDS             27843..29414
                     /locus_tag="PKU82_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ81626.1"
                     /translation="MMTGRLQSFSKPLFFAGVLLFAVGLPLSMFLMSVSQALIIVAWL
                     LNGDLKEKLKTALSNKIVLLLVVFFLWHLLGLLYTTDFHYGFKDVRVKLPLLLIPIIF
                     SSMPVFSDKQFKTVALVFIASVLVSTLISMCIYVGIIPVEFHNVRDISIFISHIRLSL
                     LICIAVYLSFWLLRKIKFSYGLLLVIIWFVFFLSLIESVTGLGILAIVLLIYAIKTFL
                     KNKSVVSRGIIAGSVVMVVTILTFTIKSAWHDVTFFNDAELRNLAKHTANGAPYTHDT
                     LNRESENGHPVWINICEPELRTAWNKRSKINYDDKNLNGDEIKLTIFRFLSSKGLTKD
                     AHAVEQLSNTEINAIEHGVANAHDLIDSNIHNRLRATLWELNRYRNGGNASGHSLTMR
                     FEFWKTALQIIKQHPLTGVGTGDVPDAFVKKYDETNSLLDAKFRLRSHNQFLAVAVAL
                     GIPALLLFIVMLFYPVFQFQYSGSFLYLTFWITAFLSMLTEDTLETQAGVSFFIFFNC
                     LFLFLKDKELDNNFI"
     gene            29469..30656
                     /locus_tag="PKU82_01755"
     CDS             29469..30656
                     /locus_tag="PKU82_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81627.1"
                     /translation="MKNYITVILFLFPFLSQAQTGEDSLHTNAANEKQTYISGYGEAM
                     VSYDSRYRTAQANFTRAVVFVGHKFSKNISFLSETELADARVEGGKAGGEIALEQAYL
                     RFQLNRKMHLQAGLFIPRIGIINENHLPTTYHGNQRPFTETFLIPSTWRELGVALYGS
                     SDAITGLNYSVALVNGLDASGFSNGTGIRGGRFEGGNATASNIALTGSVQYVMKNFTF
                     QLSAYYGGSAGLTKREADSLQLNSGMFGTPVGLGEVNVQYRKDRLTAKVLFAAITIPD
                     ADKINRAYANNTAELSVGGYAEVAYRILKKLKTDAFVRYENFDLNSSIPKNGIKNDIL
                     KQQYIIAGFSCFPTPGVVVKLDYVYKQTGDLNPALNLNPFPNAPAYYTTNSFINLGIG
                     YSF"
     gene            30659..31081
                     /locus_tag="PKU82_01760"
     CDS             30659..31081
                     /locus_tag="PKU82_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOZ81628.1"
                     /translation="MKRRNFIKNTCMSCGMMAAGGSLLSLLSSCKSTSIFKAEAVNHQ
                     LTVPLSAMNDKKILIVRNASSEYDILLVKKPDNHFTALEMKCTHRANPLNASDGGLYC
                     TLHGSRFDLDGNVVAEPATQPLKKYPVVVNNSLIQISI"
     gene            31089..32138
                     /locus_tag="PKU82_01765"
     CDS             31089..32138
                     /locus_tag="PKU82_01765"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HOZ81629.1"
                     /translation="MQFRFILFSLASVLVLSSCKKDKSNSDDYSSTQILNDFSVNVAQ
                     ATYNDLASKTEQLHQAITNFTQNQTQANLDNCKSLWKQSRQAWEQSEGFLFGPVATDD
                     IDPRIDTWPVDFNSLDSVLNNQSVYDEAYVNSLEDALKGFHPVEYLLWGVDGNKTIAQ
                     FTSRQLDYLEALAANLKTLTQQVASSWTTGNYHNEFANAGNGSQVFTTQHAAFMEMVS
                     AMAGICEEVADGKMEDPLVSQNAMLEESPFAKNSIIDFTNNIKSVENVYLGKYTSQGQ
                     SLESFVRANNLSLDGSIKTKISAAITALGNITVPFGQAIFTQQTQVINAQNAIRDLKS
                     VLENDLANLVSQKIQ"
     gene            32141..33520
                     /locus_tag="PKU82_01770"
     CDS             32141..33520
                     /locus_tag="PKU82_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOZ81630.1"
                     /translation="MKRNIVVVIIFLLLFGAQSCRKAEVLSESEWNEWLAGGKQTLFD
                     ESAGAFSSVFPDISAHNAMIHEMGDKAFEATFVSAPAPLNPGLGPIFNNVSCASCHIA
                     DGRGKPPLNSSEPLSAMLIRVSIPGVASNGGPLAVPGFGVQLQQRSINGVAKEADVII
                     NYSEQTFSFPDGETYSLRTPTYQLANSYIPLPAGVMLSPRMAPPVFGLGLLEAVDESE
                     ILKFADENDANGDGISGKPNYVWNVLAGTRTLGRFGWKANNPTVLQQTAGAYNEDMGI
                     TSYVFPAESSKGQTQYDNRNDDPEIPDSVLRAVSFYVQTLAVPVRRSADDATVLQGKK
                     LFNDAKCGSCHRPAMQTGVRVDFPEVSNQRIFPYTDLLLHDMGEGLADHRPDFDANGN
                     EWRTAPLWGIGLTQKVNGHNFYLHDGRARSLQEAILWHGGEAESAKNAFKNMSKSDRQ
                     ALIKFLQSL"
     gene            33961..35670
                     /locus_tag="PKU82_01775"
     CDS             33961..35670
                     /locus_tag="PKU82_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOZ81631.1"
                     /translation="MKAFHFLKYNESTSIDKLIAQSAPETVLCFDLEDSIQDCLDSGK
                     TTVLKKIFREYFRSMLVACKTDDGKLNIGVRINSTEGMEYNLDLLSLSGTGQISTVFL
                     PKVESAQQILKLQNDLQENNISFDEIIPVIETKVGLKNLSSILKSDLKKVKRFAFGHC
                     DYNNDCGNFPFFHQDSREYWTWITKIFKELKLYGYTIVNSPCLQLDNEVYFLNMLNIL
                     VSICGSDCAQVTLTKTQTEVCVSYSVIKPDAELPLMGNRLDFKVPAVYIESFIKEFEN
                     KYHEKGFVISNGRVLISPQEYVAALNYRKKANLPEINFTFLGGCFPVQGNMPFENRFH
                     QLLKDKVENCRNVKFNINIIRYERFKNCFNKIETYNKSHPIDVLVVSIRPEPVLRLIK
                     LYYKFKKNPGGRIERSLNIPLLNKIDAESYDLLSASTRFNPVENTANSKMHSALVDLN
                     YFFGMLIGNYSYALEKYKELLKDIIKFTNNRNIQLIVLGPPERTNALFEKLITLRLEK
                     DIKKYLEYIPECFVSGRSQNYKGEILFDSLGVYATKAYHLMIAQALYEKILPNIDDIN
                     IRK"
BASE COUNT        11640 a         6133 c         6961 g        11047 t
ORIGIN      
        1 tcatttcgga cacctgaaac gtaagtggaa tcctaaaatg gctccttata tttttatgga
       61 gcgcaatggc attcacatta ttgatttgaa taagacagct gctaaacttg aagaagctac
      121 tgctgccata aaacaaattg caaagtcagg caaaaaaata ttgtttgttg ctactaagaa
      181 acaagcaaaa gaaattgttg ccaatgcagc caaatctgtt ggcatgccat atatcactga
      241 gcgttggccc ggtggtatgc ttaccaactt tgcaacacag cgcaagtcag ttaagaaaat
      301 gacaggcttt gatcgtttca tgaaagacga atctttcaac aacatatcaa agaaagagcg
      361 tcttcaggta tcgcgtcaga aagcgaaaat ggaaaaactt atcggtagta ttgccgacct
      421 tacccgtttg cctgctgcac tttttattgt tgacgtttta aaagagcata ttgcagtttc
      481 agaagcacag aaattaggaa tccctactat tgctatctgc gacaccaata cagaccctac
      541 gttaattgac ttccctgttc cggcaaatga tgatgcaact aaatcaattt ctttaattat
      601 gaatgccatg attaaggcta ttgaagaagg tcttgaagaa cgtaaaatgg acaaagaagc
      661 tgaccgcgaa cgcgaagctg ctgcagaaga agaaggtggc gaaagaggcg aaggcgaaaa
      721 agaatttacc atgtctgacg aaggtgatga tagtggagaa acaaaaacaa aatctcctgc
      781 aaaaaaagcc accacacgtc cacgtaaagg taccacaacc agcaaaagat aaagcagatt
      841 aatttagttt aagatttaaa tattaataat ataaatgagt acagtaacaa ttagtgcaag
      901 cgatgtaaac aaactgcgtc aaatgaccgg tgcaggaatg atggattgca aaaaagcact
      961 gatggaagcc aatggcgatt ttgaaaaagc aattgatgaa cttcgtaaaa aaggacagaa
     1021 agtagcagct aacagagcag accgcgaagc aaaagaagga tacattgttg ctaaaacaaa
     1081 taacaatgct actaaaggat atttagttgc attaagttgc gaaacagact ttgtagctaa
     1141 aaatgcagag tttgtaaaat ttgctgaaga tgttcttgaa acagcattga caaataatgc
     1201 agcaagtctt gatgcattgc tggctttaca attacaaggc acaacaatca gtgagaagct
     1261 gattgaaatg gttggaaaaa ttggtgagaa gattgaaatt actcgttatg aagtaatcga
     1321 agctcctaaa gtagtggttt atattcaccc cggaaacaga gtagtttcta tggtaggttt
     1381 atcatctgct actgcagcta atgaagccgg taaagatgtg gctatgcaaa ttgccgcaat
     1441 gaatcctgtt gcaattgata aagacgatgt ggatcaaagt acattagatc gcgaaataga
     1501 aatcggtaaa gagcaggcac gtgcagaagg caaaccggag aatatgattg agaagatagc
     1561 aatgggtaaa ctgaataaat tttatcagga atcaacattg ttgaatcaag cttttataaa
     1621 agaagataaa aaaaccatcc gtcagtattt ggatggagtg gaaaaagggc ttaccgtaaa
     1681 agctttcaga cgtgttcaga ttggttaatt aaaccaaaac aatataaagg ccactccaaa
     1741 agggtggctt tttttatgga gacataaaaa aaccccgtca gttttgctga cgggggaagt
     1801 taaacccacc ctgaactaaa aatattttaa cagagaataa tctttaatca atgagtcaaa
     1861 aatagatatg cattgctgtg ccgacaaata gtttgtcctc atcgggaaat tgtgagcacc
     1921 taagcaggaa aaactactgt cagaccgaaa gggataattg caagcggcaa catgtatctt
     1981 tgcaatcaaa tttaaaaaca atggcgttat cagagcaaga acaaattaga cgagaaaagc
     2041 tgaatgagct cttacagatg ggaattaatc cctatccggc agagaagttt gaaataaaca
     2101 caaacagcac ctttattctt gaaaactacg aacaggcaga agcacgtttt ggtgcaggaa
     2161 aagaagaggt gagtattggt ggccgcatca tgagtgtaag agatatgggt aaggctgcgt
     2221 ttgcacagat acaggatgca gaaggaaaaa ttcagatata cataaaacgt gatgaaattt
     2281 gtaaaggtga tgataaaact ttgtatgata ctgtttggaa aaaacttatt gatataggtg
     2341 atattattgg cattaaaggc tacgttttta aaactaaaac aggagaaact tctatacatg
     2401 caagttcctt tatactgttg tgtaagtcac ttcgtccatt acctattgta aaagaaaaag
     2461 acggagaagt ttttgatgcc gttatagatc ctgatttaag ataccgtcag cgttatgttg
     2521 atttaattgt aaatccgcag gtaaaagaaa cattccgtaa acgtacacga ttggtgaata
     2581 ccatgcgcaa ttacctgaac gaaaaaggtt atctcgaagt agaaacacca attttacaac
     2641 ctttatatgg tggtgctgca gcgcgtccat ttaaaacgca tcacaataca ttggacatga
     2701 cattgtattt gcgcattgcc aacgaacttt atctgaaaag attaattgtt ggtggctatg
     2761 atggtgtgta tgagttttcg aaagatttca gaaacgaagg tatgagcagg tttcataatc
     2821 cggagtttac acagatagaa ctttatgtag cttataaaga ttacgagtgg atgatgaacc
     2881 tggtggagga aatgattgag aaagtagctt tagaattgca tggctcaact gctgtgaaag
     2941 taggagagaa gacaattgat tttaaacgcc catggaaacg atttaccatg tatgaagcta
     3001 ttgagcattt tacaggtgta gatgtcagtg aaatggatga aatggctatg cgcaaagcag
     3061 cagcacaaat gggaattgag ttggatgata caatggcaag aggaaaaatt attgatgaaa
     3121 tttttggaag caagtgcgaa gccaacctca ttcaacctac gtttattaca gactatcctg
     3181 ttgaaatgtc gccattggca aagaagcatc gtagcaaacc cggattggtc gaacgctttg
     3241 aagcaatatg taatggtaaa gaaatgtgca atgcattttc tgaattgaat gatccgattg
     3301 atcagcgtaa aagatttgaa gaacaacttg aacttggcaa acgtggcgat gaggaagcaa
     3361 tgcaacttga cgaagatttt cttcgcgcca ttgaatatgg tatgccgcca acagcaggtt
     3421 taggaatagg aattgatcgt ttgagtatga ttatgacaaa cgctgccagc attcaggatg
     3481 tgctgttttt cccacagatg aagcccgaaa ctattgaaac caatgcagca acggatacag
     3541 gattgtcgca catacctgcg ccttggaatg atgttatttc cagaatggga atcacttcaa
     3601 aacaacagct caaagaagct aatcctaaca aattgtttaa cgacatgggt ggcatgcgta
     3661 aaaaactgaa acttgatata agcatggtta cacttgaaga agtgaaaagc tggattaatg
     3721 tttaaaagca tcctgaagta atatcttcaa tacaccatca attaagttat aaaataaatt
     3781 tgttcatttg catgcttgtt tataaactat ggcaacaaaa agaattgatt taaaagcatt
     3841 ccgaattaaa tctgggagaa acagaaaaaa gtacacaact tttctgaaag gactgcatag
     3901 ccgcaaggta aagcagttga cagcaaaagc aaagaaactt aatgatcttg cttttaaaaa
     3961 aattgattgt cttgcctgtg gtaattgttg taagactatg acaccaacat ggcaacctgc
     4021 agatataaaa agagttgcag cacattttaa aatgacagtg caacagttta aagacaagtg
     4081 gctgtataaa gatgaaactg gcgactggat gaatacaaaa acaccatgtc agtttttaca
     4141 aagagataat aagtgcagta tttatgaaat tcgtcctaag gactgttccg gatttccaca
     4201 tctgcaacac acagactttt taattggtcg cgaagtaaat attccaaata tgaccagttg
     4261 ccctattacc tatgaagttg tagagcgtat gtataaatta gttgtagaga aagatgcaac
     4321 ggtactgaac gactaagtac tttatcattc tacttctact tcatcaacaa aaataaaagc
     4381 ttcgccaccc attggaaaac cctgatgcca tgaaggtagt tttctgaaat ttttagcaac
     4441 aacttttatg taacgtgctg ttattagagc ttcggtttgt aatttaaaat tctcaatact
     4501 tatttcataa ttatccggtg cagtctgatg ctgaacaata cctgcactat taaataactt
     4561 accatcatca gatgtaaagt actccactga aataggcata agaatccagg ctcttgtatc
     4621 ttgcaaaaaa ctggtgttaa aagaagaaac ttcttgctga ttacccatat caacaacaca
     4681 ttcaaaatcc tgcgactgat aaccttgcca gcgaccttta cgccagttta tatcaccatg
     4741 cagtccatca atcaatccgt ttgcaccgcc tgcattatat tgtggattgt aatttccttt
     4801 aatagaaact ttccattttg gatgaggaag tttaaagtac tcagcttttg cccacttact
     4861 ttcaataccg cttgagtctg taacaaaagc atgaattact gacgtatcat aaatataaaa
     4921 tggtttttta taatcaatct ttgccggacg ttcaaatggt ccttgttgca gataaactat
     4981 gtttttatct ttttgaaaag atttaatact gactttaaga gaatctttaa ataccgttgt
     5041 tgaagcagta attataggaa cacgaaataa atcatcggaa tagtaaatac tatcagccaa
     5101 atctttttta cgaagtatag aaggaatact gtcggcagtt agtactaaaa gattgtcagt
     5161 gctaaaacga ttgtgattaa aaagcaaacc atcccgaaaa gaaatatttt ttgttttctt
     5221 cactctaagg aaatttgctt tgtttgtttt gttctttaca tcaatagtaa atgttttatt
     5281 tcctgaagtt aaaatttcaa ctttatcaaa ctgaggagta gtcagcgtat attgattgct
     5341 tcccggacaa acagggtaca tgccaagagc actcatcaca taccatgcac tcatctgtcc
     5401 gcaatcttca ttgcctatta aaccatccgg actgttcttg taaaaatcat tgattatttt
     5461 attgacaatt ttctcagtct ttcccggctt gtcaacataa ttgtacaagt aagcaatgtg
     5521 atgactaggt tcgttaccgt gtgcatactg tcctattaat cctgtaatat cagattgttc
     5581 tctacctgta gtttttgtag aagcactaaa aagctgatct aatttttttt cgaatttttc
     5641 ttttccgcca ttgtatgaaa tcataccatc aatatcctga ggaacaaaaa agttgtattg
     5701 ccatccattt gcttcagtat aattgttgtt cacttcaaaa gggtcaaaag gatttagcca
     5761 gccaccgttg cttcttggcc ggataaatcc cgtcacaggg tcaaataaat ttttccatga
     5821 ctgcgagcgt tgcatgtagg tgttgtaatc atcttctttt cccaacagtt ttgccatttg
     5881 tgcaatgcac cagtcatcat aagcatactc taatgttttt gaaacagatt catgctcatc
     5941 ttccgcttca agaaaacctt tgtcaatata tgcacctaat ccaaaacggt tgcgttgttc
     6001 tgcactaaac ttgcatgctt caaatgcttt ttcatagtca aaacctttaa tgcctttcat
     6061 cattgcatcg gcaataacac tcactgcatg atagccaatc atacattctg tttcattagc
     6121 tgccagttcc caaacaggaa gtaagcctcc ttgctcatac tgcaataaaa aagtacggat
     6181 aaaatcaact gtgcgtgcac tatcaattat agtcatcaac ggatgccatg tcctgaatgt
     6241 atcccacaac gaaaagacag tataataatt atggtcagtc tgatgaattt gtaaatctct
     6301 tccacggtat ctgccatcaa catcgttata aatattggga tgcatcatgc agtgatacat
     6361 tgctgtataa aaaacggtta acttattttt atcgctgctg ctgactttta ttttcgatag
     6421 ttcttcattc catttttgtg cagcatttat ttttatgtgt tcaaaattcc agtcgggatt
     6481 ttcgagaatc atatttcgtt cagcaccatc atcgtcaaca gcagataatg caaccttagc
     6541 catgatactt tctttttcag aaactgaaaa aacgaacaca gcttttttgt ttcctaattg
     6601 ttttgattga ataaaaggtt ttgaaaaacg aatgttggca tataggattt gttcctttgc
     6661 ccagccttcg ctttttctgt aaactttaat agtggaatcg taaacaatat taatgttact
     6721 tgctgttaac ttatcacgat gatttaaatc aagatagata taagctaatc cgggtttgtt
     6781 aaatgtataa cgatgcattc ctgttcttag ggttgatgtt aattctacat caatactgtc
     6841 ggcaagcaat tttacagaat aataaccggg ggtggcattt tcgaagttgt gtgagaaagt
     6901 tgcagtggga acactttcat ccaatgcagg ctcatcgttc atgggtagta ataaaatatc
     6961 accataatca ctcacaccag taccactaag atgcgtgtgc gaaaatccat ataatacaga
     7021 atcagaataa tgataaccac tacatccatc ccaacttcca tcaatgcgtg tgtcaggact
     7081 cagttgaacc ataccgaagg ggagtgttgc tcccggaaaa gtatgtccat gaccacctgt
     7141 tcctataaat ggatttacat attgcgtgta agttggctgt gcataaagta tgctgttgca
     7201 aagcaaccag caacttagta aagcagtttt aaaaaacgac ataagaagaa gtaataatca
     7261 gataaaagta taaatagttt tgatatagta aacagaggtt attttcaggc ttttacaggc
     7321 aggaaggtag tgtataattt atttcaaatt gggctgttca gcccattgca acgggcttgc
     7381 attgagtgca cctttgtaag gtttttaata acaattgtga attacacact attatatttc
     7441 ttagaaaaac agcataaacc ttttttaatc acagcttcat ctgtattcac gtacaacaaa
     7501 ccactgctaa ctttaagcac ttcagaaatt tttaacttat aatttttata attaaaataa
     7561 ttaaatcaat ataaaatgaa aagaacaata caatcgctaa tctttattgc agttacatta
     7621 ttaaccacaa acatttttgc acaaagttgg cagttagtag gtccggttgg atttaccagc
     7681 tttaatgcca aatatacaaa tattgcattc aataataatg atgagcctta tgtggttttt
     7741 tcagatggtt cagttaatgc aaaagcatcg gtaatgaaat ttgacggcag caattgggtg
     7801 tatgtaggga ctgccggatt ttctcaaggc attgcagact atacagatat tgcttttgac
     7861 aataacggta caccgtatgt agcatacagt gatcatgcaa ataatgataa tgtaacggtt
     7921 atgaaatttg atggcagcag ttgggtagct gtgggtaatg ttggattttc aactggtgaa
     7981 ccttattaca attcattagc catcaacagc aatggagttc cttatgtcgt tttccaagat
     8041 gcaacaagtt tatctaaagc aacagtaatg aagtttgatg gcaatagttg ggttgttgtt
     8101 ggtactgccg gattttccag cgtatcggtt aaagagccaa caattgcaat agataataat
     8161 aacactcctt atgtggcctt tacacaatgg ggtggcttat ataatgcatc tgttatgaaa
     8221 tttgatggta gtaattgggt aaacgttggt ccggttggtt ttacaacaaa ttctgcagac
     8281 tatgcttcat tggcatttag caatagcgga gagccttata tggcttttat tgatggcacg
     8341 caaaatagcc atgtttctgt aatgaagttt aatggcagca gttgggcatt ggtaggaaat
     8401 gcaggttttt ctacaggaac ttcacactat ccatctttag cattcagcaa tacgggagaa
     8461 ccctatgtgg catttaagag tagctttaac aattctcagg ctacagtgat gaagtatgat
     8521 ggcagtgctt gggtggctgt ggggtcaaca ggcttttcat caggatgggc agattataca
     8581 tctcttgcat tcaacagtag cggtgtgcct tatgttgctt ttgaggatat aaataactcg
     8641 tatagagcaa cagtgatgaa atttggtaat ggaacatcaa gcataaacag catggctgca
     8701 gataatagta tagaagttta tcctaatccg gctaatgact atctgaatct tactaatctt
     8761 ccggttggtg ctaatatcaa aatcaatgat gcaataggca agaatgttta tgatgcttca
     8821 attacaacta aggaaacaag catcaacacc tctgcattta caaatggtat ttatcatatc
     8881 tgtattgaaa aagatggtgt tcttatcaat aaaaaattgg tgataaataa gtaatttatt
     8941 ttataaaaag taaaccccat ttcctgatgg aaatggggtt tttcattttc tgagaaagcc
     9001 attgttcact aaaggatatt aattacgtct gagaaatata ccggtagctt gaatgctact
     9061 ttgcatttgt tcagatttgt aaatgcaata atttaaaaat ttctttttgc gacaattctt
     9121 ttaattcacc cggctgataa tctttaatat taatagcttc aattttactt ctgattagtc
     9181 ttagagtagg aataccaaca gcagcagtca tttttctaac ctgacggttt ttaccttctg
     9241 taagaaccaa ttctatccag cttgttggaa tgtgtttgcg aaaacgcaca ggcggatttc
     9301 tttcaggata agaaacctca ttcaataaat ttacagatgc aggaggaaac gtcttatatg
     9361 gcttgccttc tatagtaatt tcaacaccct ggcgaagtag gtttaggtgt acacttgtgg
     9421 ctgtaccatc aacctgcaca tgataggttc gtttatgttt gttttcggga tttaataaat
     9481 gatgtttcag gaaattatca ttggtaagca ataataaacc ttcgctatca acatcaagtc
     9541 tgcctgcagg ataaacatcc ggttcaacat caatgaggtg tttaagtcct ttgtgtttgg
     9601 cttcatcact aaactgcgat agataaccat aaggtttata aagtacataa tattttttca
     9661 cgagttaaaa atactggttt tgattcattg aaaatgaaat actttaagca ttattaaatc
     9721 ctgacacata atgttgatta atttttatct ttgttaaact tgattttgaa atgtaaagga
     9781 gtcaataccg cttaaatgcc tttaaaatgg tagatgttca ggaactaaaa aatatacttt
     9841 gcagatacta taattctgtt agtgataaaa ccattgataa aatcgcacag cattttgaat
     9901 acagaaacta taaaaagggc gatacagtag tttcaaataa aagtatttgc acacaatgct
     9961 tttttatttg ctccggctat acaagaggat atatttatag agaagatgct cagtttacgc
    10021 tctggtttgg ggagcctggc gatatgatta cttcattttc aaccatgttt cagaacgaaa
    10081 aagggaatga aattattgaa acactgaccg actgtactat gcttgctata aacatgcaaa
    10141 catttaaaaa gttgatttcc gaagaaatag aaatagctaa tgtttatcat aaaattgttg
    10201 aagcaggata tcttttttgg gaacgaagat ttttgattta ttcacaagaa cgtgcagaag
    10261 acagatataa ggaatggtat aatcgctcaa aacatatttc agaacatatt tcgcttggtg
    10321 tgattgcaca gtatttgaat attgatcagg caacattaag ccgcatccgt tctaaaataa
    10381 aaaagtaatt ttttttttgg ttaactgcaa aagggaaaaa gggtagtttt agtatttgat
    10441 tagctttaca tgttgtaaaa tttctcctga ttcatttgca acttcaacca tataagcacc
    10501 acttgtcaga ttatcagaaa aatcaatttt tgcaagactc tgaccttctt ttaaatctat
    10561 ttgtttttga atttgtttac ctaacattga attgaaagta agcgtgtaaa gcccaccttt
    10621 acttatgttg aattgtaaat tcaactctga attgaaaggg ttgggaaata tacttagcga
    10681 acctgttgca gaacgatcta caaaaatgat tgggctgtat gaaaatgaac catcataatc
    10741 aacctgcata agtcggtagt aggtaataac agcagcaggg ttcaaatcaa tgaaatcata
    10801 attttttaat tgattcgtat tgccatgccc tttaatactg tttatagtgc gccaatttgt
    10861 agcatcggtg ctgctttcta tattaaaata atcattattt ttttctgatg ctgtactcca
    10921 gaacaaatga ttgcctgcat tttctccctt gcccttgaaa ctgattaact caacaggtaa
    10981 tgtaatgttt gacgtaaact gaatactaaa atcacaacaa tcaccttcat aaccatcaac
    11041 cataaaataa taaaatttac ctgcagacaa tccattgaca cttgcactga gacaaccttt
    11101 tggattacta catgaaccac agtctgtgcc gctaagttgc tttaaacaat aatagccatc
    11161 aatagaacct gaaccgcatg tccccaatcc atttgctgta gggaaattaa aaatcaatgc
    11221 ctgaattcct gatgcaccaa ttatgttgtt ctttcttgta catcccgtct tagtacatat
    11281 tgtgaaattt actgatgatg cagttgcctg aaaaatgtag aaaccattat tttctatggt
    11341 ttgtttgcta actgaattac aaaattcatt actgttttta aaccatgctg catcatttgc
    11401 tgtggcattg catgtatttc cgcaataaac accattaagc tgaattgcag gtgctgttgc
    11461 gcaattgtca gatggaggag ggtagcctac agtacaggtt tgactgtaac ctattgatac
    11521 cagtaatagg aaaaatgcaa atagaatggt tcggtttttc ataatctaca ttttatggta
    11581 aatctgactg tataaaagta gaattttaaa aagtgatttg atttgatgta ggtcaaaaaa
    11641 tgaaaatttg taattcccct aaaaacttaa gcttttttat caatttaaaa agggtgggtg
    11701 taagataaaa agcacttgat gccatagaac attagtgctg ttttatcata ggaaaattgt
    11761 tgtttaatat agtacaacta ttcaagtaca gtgtaaaaat tcaatgcaat tgttttgttc
    11821 aaatgattgg tatgctgtat atttgcacct tgttcttttc aaaatgctta tcgagaaagg
    11881 cggagggaag tgccctgcga tgccttgaca accatctgcc tctggcagaa aaggtgccaa
    11941 ttcacattcc ctaagggaag cagataagtc gaaatcagat taaaaacgtt caatcttttt
    12001 cggcttttcg gaaaagattt tttttatttt aaatctgatt taaattaagg agatggactc
    12061 cattgagatt gtaaaaacaa aagaaggtat tgatacgcta atcaacaccc ggattaatga
    12121 acactatcat tcattgcatg gagccatgca ggaaagtatg catgtgttta ttagaaatgg
    12181 tttgcttgcc ttgaataaaa gccttcctga cattaaaatt cttgaaatgg gatttggcac
    12241 aggactcaat gccatactca catacagaga atgttatgtg ttgcagaagc caattcatta
    12301 taccactatt gagtcatcgc ccttgccaat ggcaataata aacaagttaa attattttga
    12361 atactttgga aaagcattgc tacccatgtt tcaaaaaatg catgactgca actggttcga
    12421 gaatgtaact tttggcgatt ttgttttgca taaaattaat gctgatatgc tggagttgaa
    12481 attagatggt ggttatgacc ttgtctatta cgatgcattt tcaccaacac accagccgga
    12541 gttatggtct tttgaagtgc ttcataaggt ttatgagtca tgcaatagag gagctatgct
    12601 ggtaacttat tgtgctaatg gtgaagtaaa gcgaacatta aaaaaagttg ggtttgctgt
    12661 tgaagcattg cccggaccaa aagggaaacg tgaaatgatc agagcttgta aatagaaaaa
    12721 cattaattag aattgttgca acactttagt gaaatgaaaa atattagaca ggagttagaa
    12781 aaaagaatat taattataga tggtgccatg ggcactatga ttcagcgata taaattaagt
    12841 gaggaagatt atcgaggtga aaggtttgca gattggaaaa gtgacttaaa ggggaataac
    12901 gatttgctct cactcaccaa gcccgaaatt attgaagcca ttcacttgga atatctcgaa
    12961 gcaggagctg atattattga aactaacact tttaatgcgc agaaaatttc attagccgat
    13021 taccacatgg agtcattggc ttatgagtta aattttgagt cggctaaaat tgctaagaag
    13081 gcggttgaat cattcaaaaa gaaacatcag tcagataaaa agttttatgt tgctggtgct
    13141 gttggtccaa caaacagaac tttgtcactc tcgcccgatg taaacaggcc gggtttccga
    13201 gcggttagct gggatcagat ggtagatgct tatacggagc agataaatgg cttggttgat
    13261 ggtggtgcag atttactttt agttgaaaca atttttgata cactcaatgc caaagcagca
    13321 ttagtagcca ttcagaatgt aatggaacaa aaaaaggttg atttgcctgt catgatttcc
    13381 ggaacaatta cagatgccag cggacgtaca ttaagcggac aaacggtgga ggcatttctg
    13441 aactctgtct cgcatgttaa tctattaagc ataggcctca attgtgcatt aggtgcaaaa
    13501 gaaatgcgcc cttatctgga agagcttagt gcgaaatcac cattttatat ttcatgctat
    13561 cccaatgcgg ggctgcccaa tcagtttggt gagtacgatg aaacaccgca tcagatgtgt
    13621 gtgcagatag atgatttttt agaaagtgga tttgtaaata ttattggtgg ttgttgcggc
    13681 accacgcccg aacacatcaa acacattgca gagcatgcaa agaatcataa gccaagagct
    13741 ataccaacca tagaacctta tctgcgattg agcggattag aagcagtaac actcaggcct
    13801 gattcaaatt ttatgaatgt aggtgaacgc accaatgtta caggctctaa aaaatttctt
    13861 cgcctgataa aagataataa gtacgatgaa gcattgagtg tagcacgcga gcaggttgaa
    13921 gggggtgcgc aggcaattga cattaacatg gacgagggta tgcttgatgc cgttgaagcc
    13981 atgacaacat ttctgaacct gattgctgca gagcctgata tttcaaaagt gccggtgatg
    14041 attgactctt caaagtttga agtgctcgaa gccggattaa aatgtattca gggaaaagga
    14101 attgtaaact ccatctcatt aaaaggtggc gaagatcaat ttatctatga agcaaagaca
    14161 gtaaaaaaat atggtgctgc agttatcgtt atgctctttg atgaaaacgg acaagccgat
    14221 agtacaccac gtagaattga aatagcaaaa cgtgcttatg atattttagt taacaaagtt
    14281 aacttccctg ctcaggatgt aattttcgac ccgaacattt ttcctgttgc cacaggactt
    14341 gaagaacatc gtcagaatgc aataagttat tttgaagcaa gtaaatggat taaagaaaat
    14401 cttcctcttg caaaaatttc aggtggtgta agtaatgttt ctttttcatt tcgtggtaat
    14461 gaccccattc gcgaagccat tcatgcagtg tttctttatt atggaataaa aaatggaatg
    14521 gatatgggca tagtaaaccc atcgcaactt atcgtttatg atgaaataga aaaagattta
    14581 cttgcattgg ttgaagatgt tatcttagac aggagagaag atgcaactga aaaactcttg
    14641 gcaaaagccg aagagttaaa actttcgcag accacaactg ataaaaatgt aaagaaagga
    14701 aatcaatcgg ccaacgcctg gcgcagtgaa gacctgcaat cacgtattac acattcattg
    14761 ataaaaggta ttgacgaatt tgtagatcag gatatggaag aggcgcggca gcaggtatct
    14821 aaagcacttg aggttattga aggtcatctg atgattggta tgggtgtggt aggtgactta
    14881 ttcggacaag gaaaaatgtt tctgcctcag gtagtaagaa gtgcacgtgt gatgaaaaag
    14941 gcagtggctt atctgacacc ttttattgaa gcagaaaaag taaaaggtca gtcaaaagga
    15001 aagattgttc tggcaacagt taaaggcgat gtacatgata ttggaaaaaa tattgttggt
    15061 gttgtacttg cctgcaacaa ttacgaaatt gttgatttgg gtgtaatggt tgcagcagat
    15121 aaaattctgc aggctgcacg cgatcatcaa gcagatatta ttggtgttag tggattgatt
    15181 actccttctc ttgatgaaat ggtacatgtt gccaaagaaa tggagcgaga aaaatttaca
    15241 atgccattgc tgattggtgg agctaccaca agtaaggtac atactgccgt aaagattgag
    15301 ccaaactatt caggcgttgt tgtgcatgtc aatgatgctt cacgaagtgt gcctgttgtg
    15361 agtaatttac tttccgcaga acaaaaagaa aaatttattg aagatataaa agcggagaat
    15421 gaaagaacac gctcctatca taaggcagag cgatcaagag gaaaattttt atctttgaaa
    15481 gaggcgcaac aaaataaata tgaattgaat ggagccgttg ttactgaacc aaaatttaaa
    15541 ggagtaaaag ttttcagcaa ctatgatttg tcattgctta cagaatatat tgactggaca
    15601 ccatttttta ttgcatggga aatgaaaggc agctatccga aaatattgag cgaccctcaa
    15661 cgaggtcagg aagcgcaaaa actttttgat gatgcacaaa caatgctgca acgtattatt
    15721 aaagaaaaat ggcttacagc aaatgctgta ttcggaattt ttcctgcagc gaacaagggt
    15781 gatgacattg aagtgtttga tgaaagaaga gaaaataaaa ttgcaacact tcatactatc
    15841 cgtcagcagt cagagagagc aacaggacag aagaatattg cattggcaga ttttattcgt
    15901 ccttcagatt atatcggagc ctttgctgtg tcaacaggtt ttggaataga aaaatggtta
    15961 aagaaatttg cagacgatca tgatgattat tcatccatca tgttaaaagt gttggccgac
    16021 cgattagccg aagcatttgc cgaacacctg catgagcggg tacgcaaaga gttttgggct
    16081 tatacaacag atgaaaatct gaatcgtcag gatttaatca aagaaaagta tcagggcatt
    16141 cgtcctgctg caggctatcc ggcacagccc gaccatacag agaaatacac actttggaaa
    16201 ctacttgatg tagaaaaaaa tacaggcatt acgcttacag aacatttggc tatgtttcca
    16261 actgctgccg tcagtggatt gtattttgca catcccgaat cgcattattt tgcagtagga
    16321 aaaattcagc gcgaccaagt tgaggattat gcaaaacgta aatcaatgac tgttgaagaa
    16381 gccgaacaat ggctgggaag tattttaggt tattaatttg aggagattat tcttacaaat
    16441 ttaattcctt caaaagctga ttttccttca accaaaaatc ctatttttgc agactaaaat
    16501 ttaaagtatg tcaaaagaca gatttcaggc accggattat tttttaatgg atgaattatt
    16561 aacggacgaa cataagctga ttcgtgacag tgtgcgcgag tgggtaaaga aagaggtttc
    16621 tccgattatt gaagatgcct gtcagaagtg tgagttccca aagcatttaa taaaaggttt
    16681 ggcagaggta ggtgcattcg gcccttatat tccggcagag tatggtggtg gtggattgga
    16741 tcagatcagc tatggtttaa ttatgcagga acttgagcgt ggcgacagtg gtatccgttc
    16801 aaccgcatcg gtacagagtt cgttagtgat gtatcctatt tatacctacg gaagcgaagc
    16861 acagcgtaaa aaatatttac ccaaacttgc atcaggagaa tggatgggat gttttggttt
    16921 aacagagcct gaccatggtt caaatcccgg tggtatggtt accaatatta aagataaagg
    16981 cgatcattat ttattgaatg gtgcaaagat gtggataagc aatgcaccgt ttgcacaggt
    17041 tgcagtagtt tgggcaaaag acgaagaagg tgtcatcaga ggcttgatag ttgaacgtgg
    17101 catgaaaggt tttacaactc ctgaaacaca tggcaaatgg agcttacgtg ctagtgcaac
    17161 aggtgagttg gtttttgata atgtagaagt gcctaaagaa aatttattgc ctaacgtaaa
    17221 aggtttaaaa ggaccattag gatgtttgaa cagtgcacgt tatggcattg cttggggagc
    17281 tttaggtgca gccatggatt gttatgacag cgctttacgc tatgcgaaag agcgtaaaca
    17341 attcggaaga ccaattggag gctttcagct tcagcagaaa aaacttgccg aaatgctgac
    17401 agaaattacc aaaggtcagt tgttggtatg gcggcttgga aaattaaaga atgaaaacag
    17461 agccaatcct gcacaaataa gtatggctaa acgtaacaac gttcacactg cattgtggtg
    17521 tgcccgcgaa gcaagacaga tacatggcgg catgggcatc acaggagaat atcctatcat
    17581 gcgccacatg atgaaccttg agtcggtaat tacttatgaa ggtacgcatg acattcattt
    17641 gctcattaca ggaatggata taacaggaga gaatgctttt acctgatcag gaaaaagtaa
    17701 ctaaagcgtt agcgcagtag ctgcagaaat ttttttgctt tgtttgtttt gtatgaatgc
    17761 aatgagttga aaattctcat tgagtgcaag tttattggtt tttaaaagca gtgttccttt
    17821 tgtggcaaca ggatatactt cgtaaagtct cactacgttg tcttgaacaa gtgttcttcc
    17881 tatgttttca ccttttgtaa tttttgttgt aacacttttt tgtactacgg ctacaataat
    17941 attagcattg gcatcggctt tacttgcagt atagttaaga tgtaaagtgt catttagggt
    18001 ttgtaaaact gtaaccgtca gtttttgttt agcaactgat ttaagacctt tttcaatttc
    18061 tgaattcaga cgtttgctgt cagagcctac aaagcctgtg ttgccgttaa caaacacttg
    18121 tggcgtaaac acttcattgc tgcctgttgc actaacataa ttattctgtc gtcttgaata
    18181 cttaatgctg ctgtatggat ctttccatcc atatttattc cagtagtcaa catgaaagct
    18241 taaaaaataa acaggcttat tattcttaat ggcatcatcc atttcttttt tcagaactgt
    18301 ttctgctgcc ggacaactgc cacaaccctg tgaggtaaaa agctctacca cagcaaaacc
    18361 gttgccggat tgttgtgcta aagcaactga tgaaaacaat gttataagaa tgaataaata
    18421 tcttttcatg aatacaaagc aataacgcaa aaatgtagaa aattatatta gtttatatgt
    18481 ggtatccatg aagttatgag caaataagat ttgattttca accattatct cgccatcgcc
    18541 acagatacat gcatgcataa gttctgtaag gtgcccattg ctcagcgata gctaaaattc
    18601 gcaggcgcaa ctttttctta tcggatattt taatcttata aagtccgata acggattgct
    18661 gaataccata atcgtcaaca gaaaaaacat cttcgcgacc tagggtaaac atcataatca
    18721 tttctgcagt ccaacggcca acacctttta tttgagtaat gtattcaata aattcgtcat
    18781 tactcatttt atttaccttg ttcaactgca aactgttttc cagtgcaaaa gctgcaatgt
    18841 ttttaatata gcttgcttta gaattactca atccaatact tcttaaagtt tctgttgttg
    18901 cctgaagtat tttttcggga gtaggctttc catcaaataa atcataaaaa cgctgtttaa
    18961 ttacgtcagc aacttttaca gacaactgtt gtgatataat agaaccgcat aaactcagaa
    19021 acaagtcttt tcttttttta agttctacag ttccatgttg cttaattacc gatgctaatt
    19081 ttttatcttt agaaagatgt ttgatgtatt ccatatttaa aatttaggct aatgtattac
    19141 acttgataaa ataatcttta ttacaacaaa catttaacat tatctaagta tctaatcaac
    19201 aaatcatcta agtaaaattc caattaaaat cctgcattcc aactttccaa acattttaaa
    19261 ccttcaaact ttcaagaagt cacaaatcag aaacttaaaa attagctgca tcagtaactt
    19321 tcaaaacatt gtaacattct aaactttcca aaccttcaaa ctttcaagct ttcaagaagt
    19381 cacaaatcag aaacttaaaa aataacgaaa tcaataactt tcaaaacatt gtaacattct
    19441 aaactttcca aaccttcaaa ctttcaagaa gtcacaaatc agaaatgtaa aaattagcga
    19501 aatcaataac tttcaaaaca ttgtaacttt caaaacattg taactttcaa aacattctaa
    19561 accttcaaac ttttcaagaa gtcacaaatc agaaacttaa aaattagctg cattaataac
    19621 attctaaaca ttcaaaactt tccaaacagt ctaaaccttc taaactcacg ttaatattct
    19681 ttaatttgca ctttcattgg ctaataacat gcagataaag aaatttacat ttaatccttt
    19741 tgaggaaaac acatatattg tttatgatga aacagcacag tgtgccatta tagatccggg
    19801 atgctatatg cctgaagaga aggaagaatt actcagtttt attgcagaac aaaaactaaa
    19861 ccctgttttg ttgctaaata cacattgcca tgtagatcat attttcggaa acaaacttat
    19921 ctatgatact ttcggattaa aaccacagtt tcatcagtta gagttaagat tgcttgaagc
    19981 agcaggagaa tacagtaagc aatggggaat atttctggag ccatcaccaa cggcagaaaa
    20041 gtatttggaa gaaggagaca tcatttcatt tggtaatatt tcattagaaa tattattaac
    20101 tcccggtcac agccccggaa gtttatgttt ttatgataaa caagagaaaa ttattattgg
    20161 aggtgatgta ttgtttcaga tgagcatcgg ccgttatgat ttacccggtg gtgatgagca
    20221 aacattgttg aatagcatca gaacaaaact gtttacactg cctgcagatg taaaagtgct
    20281 tccgggtcat ggccccgaaa caacaatagg ttttgaaaaa atgtacaatc cttttttagt
    20341 ttaaggtgca ttaagcaagg aatgcccgag cgtttcgctc gcgcattctc tgctttaaac
    20401 attacaactt ttccaaccct ccaaccttcc aaactttcca accttccaaa ctttccaacc
    20461 cttccaaact aaagcctatt tcttacactc atcaagcatt ttctgagcaa actcttttcc
    20521 ccagtttggc atcatagcgt tggcaggttt aaatgcatta aatttctcta ttgccttttc
    20581 aagaacaggt aatgctttgg ctttgccacc accaaattgt tcaggagtaa acaacaatcc
    20641 tgtaccttgc agaaaatagg gacgtggatt ttccggattc agttcaatgg catgtgctgt
    20701 aagcatgcct gcttccatcc ctaatttttg tccgcgtgtt gcagggtcaa taccaatttt
    20761 cattgaggtt atccacgatt gcagtgtaac aatctctgaa ttatcaggtg agagtttatc
    20821 tgctttattg gcaatttcaa gcgctttgtc taaaacggca tctttcttta cattgtcttt
    20881 ttcatcattt gaagaaagta ctaaagcata agcctgatag tataaaggca gccactcctt
    20941 ggtttctgca tttgcaatgc gctcaaatgc attggcggca ttcacaaagt cagtacctgc
    21001 tttggcttta tcaagcaaat caatttgttt aaccatagtt ttttcaaact tgctgttgct
    21061 ttgtgcaaac atgttgcctg caataaagag taaaaggaat gtgagttttt tcattttata
    21121 atttgatttg aattttttta ttctgttctg tcttctccga aactgataaa tacaccaata
    21181 aaaaaggagc gtttggaagt cgggccaaca gcacttcttc ttagcccgtc agtgctgtaa
    21241 cgatagctgt atgcactttc aatacccaac acattattta cggcagcaga aattactgta
    21301 aagtgtctgc caatggatgt taaatagctc caggttaaac tcaggttgtt ataatcgtta
    21361 gtgcgatcgg aaagaaaatt attttcattg gttttattag gattataata aggtcttcct
    21421 gctgcataca cataggtgaa accaatgctg cttcgcaatt ttggaaacca gcgtttcatc
    21481 actacactca ccacatgctt tgaagcaaag gtgggctgca cttcaaacgg atagtataaa
    21541 tattttcttt tggtgtcgag gaatgaatag gaaatccaat aatccacata tttaaaagtt
    21601 tgtttatcgc gccagaaaaa atctattcct tgtgcatagc cttttccatc atcaccggta
    21661 gcaaatgctg tagcatatac atccgatgcc atatttgtag tctgcactaa gtggttgtat
    21721 tttttataat atgcttcaac acgaaaggtt cttttttcat ttacatactg atagttcaaa
    21781 atgtaatgtg ttgctttttc aaaatgatcg aggttggtaa agatgagata atttttatcg
    21841 ggtgtttgat aataatctcc ataggcaata ttcaactgat caaactttcc taatttataa
    21901 gccacgctga tgcgtggtgc aagattactt gcacgaagca ggtatgaatt ttcaactctt
    21961 agtccggcac gagctactaa ttttcttgtg atgtaggcat cgccttcaag aaaaactgcc
    22021 gtaaacattt ctgtactatc cactctgaaa ttattataga ggttactgaa gtcattatat
    22081 tttacttcta cgccaccacg tatggagctc aattcactta ttgaacggga cactactgca
    22141 cgcccttgcc acagttgatt actgaaaagc agatgttgtg gccttgcata aatacgatta
    22201 atatccttgc tgtaagatcc tgctgcctgc actgtccatt tattgagcac ttctttatat
    22261 gaaagcgtag aaaagatgtt gtagttgttg agaccaaaat gatttttact ttttccggta
    22321 acgtcttcca gattgtcgaa gttgagcgag aggtcgctgt actgataatt gataaatgct
    22381 ttaataattc ctgtttcaga tgtcttatgt ctgaacactg cagaactgct gaaagtttgt
    22441 ggaggagaat cccagtcgcg gttttgtttg atgatagcaa aatagggtgc aaggttaaaa
    22501 taattggcaa atgcacctgc tgaagttttt ttccaacgat gcatgtaacc accaccacca
    22561 ccaacagaca tgatgccaat gtttccgaca gagcgtggag gaaggtcttc gctttctaat
    22621 gcaagcaccg atgacatggc ctgaccatat tgcgcagaat aacctccggt gctgaagttt
    22681 gtgcctttaa acataaaagg agaaaacctg ctgcgttgcg gaacatcagg aacacttgag
    22741 aaatatgggt ttgcaacctc tattccatca ataagtgttt tggcttctgc tgcatcgcca
    22801 ccacgcacaa aaagtccttc ctgatcttgt gtaggctgtg tgccgggaag tgtttgcagc
    22861 gctccgtaaa tgtcaccctg cgaacctgct gttgtaacaa tatccaatgg tcgtaaaatg
    22921 accattttct tttcatcact tgcctcaaag gctcctgctg ttatagttac tgtattcagc
    22981 tcattgcttg tttctttgag tttgaaattt atttcaagtg tgttaccttg tagttttatt
    23041 tttttatctg aaggactaaa accaatcagg ctgcaaacta ttatcacatc accggaatca
    23101 tttgcattga attgatattt ccccgaagca tcgctgctgg caccatcata actgtcgcgg
    23161 ataacgatgt tggctcctgc aacaggatta tttttctgat cggtaactgt tcctgaaatt
    23221 gtagtttgtg cggttgctga ttgtatgata attaaaaaaa acaaccaact gattacatgc
    23281 ttaggtaaat acatttgatt cggttatggt taaacaaagg taaaagattt aattgttttg
    23341 tcaagatatt atttttttgt cagtgtctgt aaatactcct gtgcagcctt tattaatttg
    23401 ttgtaccatg cttcgccata ttttctggtc agtgcagttt tcagaaattc ataaacccga
    23461 actttttctt tctttcccaa cttacaggca ggactgcaaa cttcccattc atgatagttt
    23521 aatgcatcac ccgattttgt ttttaaaaca cgtataggat aaagatgaca actgattggc
    23581 ttataaaatt ttattttttt atctcgatat gctttttcaa ttccgcatag tgcaatacca
    23641 ttttcaaaaa cggtgtaagc gcatgggcca ccgggccaca tcaaaggtgt cacccattca
    23701 tcatcatcat ctttcagatg aaatccctgc gcttcaatag cttttattcc ctcatcggtt
    23761 aaataaggtt taactttggg ataaactttt tccaatgctt tcaactcatc ttcttctaat
    23821 ggcgcgcccg attcaccttc tacacaacat gctcctttac atttagaaag atcgcaaata
    23881 aatttctctg taataacctg gtccgatacc agtatgtcgt caacttttaa caccggttta
    23941 tttttggcaa aggtaaaact tcatggcatt ttgaggaagg aagtcaaatg ttataacttt
    24001 gcacatcatg aatccaatta taacggtaaa taatttagtc aaacattacg atagctttca
    24061 cgctgttaaa ggaatatctt ttgaagtgta tgaaggtgag atttttggtt tactcggacc
    24121 taatggtgcc ggtaaaacga caacgcttga aataatggaa acccttcgtg ccaaaacatc
    24181 aggtgacata actattaaag gaaataaagt tggtactgat gatgatgcga taaaaaaaat
    24241 aattggcgta cagttgcagg ctgcaggcta ttatccaagt ctttcattac ttgagttgat
    24301 tgacctattt gccggattgt acagtcagga tgttaatgca atggaattac ttcagaaagt
    24361 aggcttaacg gataaggcaa aatcaaaata caaagaattg tcaggtgggc agaaacagcg
    24421 attttctatt gcaacaacat taatcaacaa acctgcagtg atttttctgg atgaacctac
    24481 tacaggatta gacccacagg caagaagaaa tctttgggac ttgattcgac agataaaaaa
    24541 tgaaggcact actgtagtta ttactacaca ctatatggac gaggcagaag agttgtgcag
    24601 cagagtagct attatcgaaa atggaaaaat tattgcactc gatactcctg accagttgat
    24661 agacgattta gtagcaaaag gttttgagcg aaaaaaagaa gttaaaaaag caaaccttga
    24721 agacgttttt ctgaatctta caggtcagga atggcgagaa taatgattgt tttttatcgc
    24781 tgatgcggta aatagttttt tgtgatttta attgtaatac taaaatcaat cagcattatc
    24841 caatacatat ttttatcggt gtaaacatca taagttgtta ctccgttttt ctgttcaaaa
    24901 atgggttcac gtgttttagc ataatagatt cctgcattag caccatagcg cacaccaatt
    24961 ctgtcggtaa gttcggtctg aatatttaaa ccgggcaata aggctgcacc attatactgt
    25021 gttgcatata aacttccagg gtaggttgat ttttcgtatt taaaatttac ataagggata
    25081 tacagtgtgg gacgaatttc taatctttcc ttaaaaaacg atagtagctt tacagaaaca
    25141 caggggccaa tagcataatt acttacttct tgtgcttttt cggagcggaa attatacatg
    25201 cctcggattg aataaatggt tttgcggctg cgcttattta cactgtagtt caaccaaaaa
    25261 ttttgagatc catgaaaaga cggataatat ccaaaaccta tgcgttgttc tttaaaaata
    25321 gtttccttag cattgatgtt gcccaacgat agtatgaaca ataaacatag tgccagcccg
    25381 gatttaagca taaataaatt ttcgccaaat tataaagtta gaaccaaaaa caatataatc
    25441 ctgaaaaata attatctgtc cgatacaatg tttgtacttt tgcaaaacaa tgaatgaggg
    25501 cactttgcga aacacatttt ctaaggcaga aaggctttgt aaaaggaaag attttgaact
    25561 gatttttagt cagggtgcag cgtttaaccg ttttccgttc agagtgcatt ggctgaagtt
    25621 aaaagaaaac acatcaacag ttcctgttaa aattgccata agtgtaccga aacgaaaaat
    25681 aagaagtgcc gtttcccgca accgtattaa aaggcttatt cgtgaaagtt acagacttaa
    25741 taagcagata ttgagcaacg aactgaaaaa aaatgagcag gtcatacata ttatgtttgt
    25801 ttataatgga aatattaacg ctaccttcac agagatacaa tctaaaataa tcctaatttt
    25861 acaacgttta aaagaattga atgatcaaac tgctgaacag gattctgata gccatagtaa
    25921 agttttataa aatggtcata tcgccattgt tgcctatgtc atgtcgttat tatcctacat
    25981 gcagcgagtt ttgtatggaa gcgctgaaaa agtatggacc gttgaaaggt ggttttttat
    26041 cactaaaaag aatattatcc tgcaatccat ttggtggcca tggacatcat ccggtgcctt
    26101 aatgattttt aaaaatatta attgattaaa tagtttataa gatgctacaa aaaatgcgta
    26161 aaagaatcag attgtatgtt gccgttattc tggttggcat atctgctttt tctgtttata
    26221 gtttcagtga cgattatttt gaaatatcgc gcaacctcga cattttcgct accttgtttc
    26281 gtgagttgaa catttattat gttgatgaaa caaatcccgg tgacctgatg aaaaaaggca
    26341 ttgatgatat gcttgcctca cttgatccat acaccaatta tattcccgaa agtgaaattg
    26401 aagactatcg tttcatgact acaggacagt atggtggcat tggctcactt atcggttcgc
    26461 gcaataacga agtaatcatt accgatcctt atgaaggatt tcctgctcaa aaagccgacc
    26521 tgcgtgcagg tgatgtaatt gttgaactag acggtaagcc cattaaagga aaaaagaatg
    26581 atgaagtgag taagctgctt aaaggtcagc caaaaacttc agttaaaata actatcagac
    26641 gtgaaggtga accaaacctg attgataaaa atgtagtacg tgaagagatt aaaataaaca
    26701 gtgtttctta cagtggaatt attgataacg atttaggtta tatccgcctt accggattta
    26761 cagaaaatgc aggacaggaa gtaaaaaatg cattgcaagc tttggaagca aagacaaaac
    26821 ttaaagggtt gatttttgat ttacgcggaa acccgggtgg attacttaac gaagcggtta
    26881 acattgtaaa tatttttgtc aataagggtg ttgaagtcgt tagcacaaaa ggcaaagcaa
    26941 aagaatggga taaaacctat catgcattga acaatcctgt tgacacagaa attcctgtag
    27001 cagttttagt aaacagcagc agcgcatctg catcagaaat tgtttcagga agtttgcagg
    27061 atttagacag aggcatcatt attggccagc gtactttcgg taaaggactt gtgcaaacta
    27121 ccagaccgtt aagttatggt gcacaactta aagttaccac agcaaaatac tatattccca
    27181 gtggtcgttg cattcaggca ctcgactatt cacatcgcaa tgacgatggc agcgtaggaa
    27241 aaattcctga ttcactcatc acacaattta aaacgaagtc tggccgtact gtttatgatg
    27301 gcggtggtgt tatgcctgat tttgttactg acgtcagaca actgagtcct atttcgcaaa
    27361 gtttattact gaaatattta ttgtttgatt atgcaacaat gtatcgtaat acacatccat
    27421 caattgcacc tgcaaaagaa tttcatctct ccgatgctga atatgctgag tttgtaaaat
    27481 ggatttctgc aaaggattat gattatgaaa ccaagagcga aaaaattctt gatgatttta
    27541 aaacaactgc agaaaaagaa aaatatttta gtgcagcagc agcagagttt gaagtgctga
    27601 agaaaaaaat atcgcatgat aagaatgcag atctacaaaa gtttaaagat gagataacag
    27661 agttgttgga gaatgaaatt gtgtcacgct attattatca gtcaggacag attgaagcat
    27721 cttttaagaa cgatttggaa attaagaagg ctacagaagc attgaagaat aaagatgtct
    27781 atagttcaat catgaaaagc tctatggctt cggttagaga gaagcactag aatactgcat
    27841 taatgatgac aggccggcta caaagtttta gtaaacctct tttttttgcc ggtgttttac
    27901 tgtttgctgt tggattgcca ctctccatgt ttttgatgag tgtttctcaa gccctcatca
    27961 ttgttgcatg gcttttgaat ggagatttga aagagaaatt aaagacagca ttatctaata
    28021 aaattgtttt actgctggtt gttttctttt tatggcatct gttgggatta ctttatacta
    28081 ccgattttca ttatggcttt aaggatgtgc gtgtcaaact tcctttgctt ttaatcccaa
    28141 ttattttttc atcaatgcct gttttttctg ataaacaatt taaaacagta gcattagttt
    28201 ttattgcatc cgttttagtg tccacattaa taagcatgtg tatttatgta ggaataatac
    28261 ctgttgagtt tcacaatgtc agagatattt caatttttat ttcacacatc agattgtcac
    28321 tccttatttg tattgctgtt tatttgtcat tttggctgtt aagaaaaatc aagtttagtt
    28381 atggattgct gcttgtaatt atctggtttg tgttttttct ttcattgatt gagtctgtca
    28441 ccggtttggg catactggca atagtgttat tgatttatgc tatcaaaaca tttctaaaga
    28501 ataaatctgt agttagcaga ggaattatag caggttcagt tgtaatggtt gttacaatac
    28561 ttacattcac tatcaaaagc gcatggcatg atgtgacatt ttttaatgat gctgagttga
    28621 gaaatttagc aaaacataca gcaaatggtg ctccatatac acatgacact ctgaatcgtg
    28681 aatcggaaaa cggacatccg gtgtggatta acatttgtga gccggaattg cggacagcat
    28741 ggaacaagag aagcaagata aattatgatg ataaaaatct gaatggtgat gagattaaat
    28801 taacaatatt ccggttttta tcttccaaag gattaacaaa agatgcacat gcagttgaac
    28861 agctcagtaa tactgagatt aatgcaatag aacatggtgt agccaatgca catgatttaa
    28921 tcgattctaa tatccacaac agattaaggg caacactttg ggagttgaac cgatatagaa
    28981 atggaggcaa tgccagcgga cattcattaa ccatgcgttt tgaattttgg aagacagcct
    29041 tgcaaatcat caaacaacat ccgttaacag gtgttggcac gggtgatgtg cctgatgctt
    29101 ttgtaaaaaa gtatgatgaa accaattcat tgttggatgc taaattccgt ttgcgttcgc
    29161 ataatcagtt tcttgctgtt gctgttgcac tcggtattcc cgctctttta ctttttatcg
    29221 tcatgctttt ttatcctgtt tttcaatttc agtattcagg gtctttctta tatctgactt
    29281 tctggattac ggcttttttg tcaatgttaa cagaagatac acttgaaaca caagctggag
    29341 ttagtttttt catttttttt aattgcctgt ttttgtttct gaaagacaaa gagttagata
    29401 ataattttat ttagaaacat tctaaatagt attttctcat tactttcgca gccgataatt
    29461 ttcattcaat gaaaaactac ataacagtca ttctgtttct atttccattc ctttctcagg
    29521 cacagacagg tgaggattca ttacatacca atgcagctaa tgaaaagcag acctacatat
    29581 caggctatgg cgaagcgatg gtaagttatg attcacgcta cagaactgct caggcaaatt
    29641 ttaccagagc tgtagttttt gtaggccata aattcagcaa gaatatttct tttctttcag
    29701 aaacagaatt ggccgatgca cgtgttgaag gtggcaaggc aggtggagaa atagcattgg
    29761 agcaagctta tctgcgtttt cagctaaaca gaaaaatgca tttgcaggcc ggattattta
    29821 ttccgcgcat tggaattatt aatgagaacc atttaccaac aacttatcat ggtaatcaac
    29881 gcccctttac agaaactttt ctaataccat caacatggcg cgagttgggt gttgccttgt
    29941 atggtagttc agatgctata acaggattaa attattctgt tgcacttgtc aacggtcttg
    30001 atgcatcggg cttcagcaat ggaactggta ttcgcggagg taggtttgag ggtggcaatg
    30061 caactgcttc taatattgct ttgacaggat cagtgcaata tgtaatgaaa aatttcactt
    30121 ttcagctttc agcttattat ggtggaagcg caggcctgac aaagagagaa gctgatagcc
    30181 ttcagttaaa ctcaggcatg tttggcaccc ctgtggggct tggcgaagta aatgttcagt
    30241 atcgcaaaga cagacttaca gcaaaagtgc tttttgctgc aatcactatt cctgatgctg
    30301 ataaaattaa tcgtgcctat gccaacaaca cagccgaatt atctgttggt ggttatgcag
    30361 aagttgctta tcgcattctc aaaaaactaa aaacagatgc ttttgtccgt tatgaaaatt
    30421 ttgatcttaa cagcagtatt ccaaaaaatg gaataaagaa cgatattctg aaacagcaat
    30481 atatcattgc aggattttct tgttttccta cacccggtgt agtggtaaaa ttagattacg
    30541 tatataagca aaccggtgat cttaatcctg cattaaacct gaatccattt ccgaatgcac
    30601 ctgcatacta tacaacaaac agttttataa atcttggcat aggctatagt ttttaaaaat
    30661 gaaacgcagg aattttataa agaacacatg tatgagttgt ggcatgatgg cagcaggagg
    30721 aagtttactg tcgctgcttt cttcttgtaa gagcacgtca atattcaaag cagaagccgt
    30781 gaatcatcaa ctgacagttc cactgtcagc aatgaacgat aaaaagattt taattgtgcg
    30841 taatgcttca tcagagtatg atatcttatt ggtaaaaaaa cctgacaatc attttactgc
    30901 cttagagatg aaatgcacac atcgcgccaa cccactcaat gcaagtgatg gcggcttgta
    30961 ctgcacactt catggcagcc gcttcgacct tgacggaaat gttgtagccg aacctgcaac
    31021 acaaccactc aaaaaatatc ctgtagtagt aaacaattca ctaattcaaa tatcaattta
    31081 atttaatcat gcaattcaga tttattttat tctcattagc atcagtgttg gttttaagtt
    31141 cctgcaaaaa agataaaagc aacagtgatg actattcttc aacacaaatt cttaatgact
    31201 tttcagttaa tgttgcacag gctacttata atgacttagc ctctaaaaca gagcagttgc
    31261 atcaggcaat tacaaacttt acccaaaatc agacacaggc aaatcttgat aactgtaaat
    31321 cgttatggaa acaaagccgc caagcatggg agcagtccga aggattttta ttcggccctg
    31381 ttgcaacaga cgatatagac ccacgtattg acacctggcc tgtagatttt aattcactcg
    31441 attcagtttt gaataatcaa tctgtttatg atgaagccta tgtcaattca cttgaagatg
    31501 ctttaaaagg atttcatcct gttgagtatt tgttgtgggg tgttgatgga aataaaacta
    31561 ttgcacagtt tacttcacgt cagttagatt acttagaagc acttgcagca aatctgaaaa
    31621 cactaacgca gcaagtagct tcaagttgga ctacaggaaa ctatcataat gagtttgcaa
    31681 atgcaggaaa tggcagtcag gtttttacta cacagcatgc ggcatttatg gaaatggtta
    31741 gtgctatggc aggtatttgc gaagaagtag cagatggaaa gatggaagat ccattagttt
    31801 cacaaaatgc tatgcttgaa gaatcgcctt ttgcaaaaaa ttcaattatt gattttacga
    31861 acaacataaa aagtgtagag aatgtttatc tgggaaaata tacttctcag gggcaatctt
    31921 tagagagctt tgtcagagcc aacaaccttt ctttagatgg ttctattaaa acgaaaatca
    31981 gtgctgctat aactgcatta ggaaatatta cagttccttt tggtcaggcc atatttacgc
    32041 aacaaacaca ggttatcaat gcacagaatg ctattcgtga tttgaaaagt gttttagaaa
    32101 atgatttagc aaatttagtt agtcagaaaa ttcaataatc ttgaaacgga acattgttgt
    32161 tgtaattatt tttctgcttt tattcggagc acagtcttgc agaaaggccg aagtgctgtc
    32221 agaatcagag tggaatgaat ggcttgcagg aggtaaacaa acactttttg acgagtctgc
    32281 gggagcattc tccagtgtgt ttcctgatat ttcagcacac aatgcaatga ttcatgaaat
    32341 gggtgacaaa gcttttgaag caacatttgt cagtgcacct gcaccattga atcccggact
    32401 tggacctatc ttcaacaatg tttcctgtgc atcatgtcat attgcagatg gcagaggtaa
    32461 gcctccgttg aatagcagtg agccactctc tgcaatgtta atcagagtaa gcatacccgg
    32521 tgtggcatca aacggtggac cattagctgt gccgggtttt ggtgtgcagt tgcaacaacg
    32581 atctattaat ggtgttgcga aagaagcaga tgttatcatt aattattctg aacaaacatt
    32641 ttcttttcct gacggtgaaa catattcatt gcgtacacca acatatcagt tagccaacag
    32701 ctacatacca ttacctgcgg gagtaatgct ttcaccacga atggcacctc ctgtatttgg
    32761 tttgggtttg ctcgaagcag ttgacgaatc tgaaatatta aaatttgctg atgaaaatga
    32821 tgccaacggt gatggcattt ccggcaagcc gaattatgtt tggaatgtac ttgcaggaac
    32881 acgcacttta ggtcgctttg gatggaaggc aaacaatcca acagtgttgc aacaaacagc
    32941 aggtgcctat aatgaagaca tgggtattac aagttatgtt tttccggcag agtcaagcaa
    33001 agggcaaaca cagtacgata acagaaacga tgaccctgaa attcctgata gcgttttgcg
    33061 tgcagtttca ttttatgtgc aaacattagc tgtgcctgta agaagaagtg ctgatgatgc
    33121 cacagtgttg caaggcaaaa aactatttaa tgatgccaag tgtggctctt gccaccgacc
    33181 tgctatgcaa accggtgttc gtgttgattt tccggaagta agtaatcaaa gaatttttcc
    33241 ttacacagat ttgttgttgc atgacatggg cgaaggtctt gcagaccacc gacctgattt
    33301 tgatgccaat ggtaatgaat ggcgtactgc accgttatgg ggtataggtt taacacagaa
    33361 ggtaaacggt cataattttt atttgcacga tggcagagca cgttcgctgc aggaagcaat
    33421 tttatggcat ggcggtgaag cagaatcggc aaagaatgct tttaaaaata tgagcaaaag
    33481 cgacagacaa gcattgataa aatttttgca gtcattataa ttcattcttt cagaattttc
    33541 ctgactgtga tgaaatgatg atggatgctc aggaattttt aaaataaatc aaaagtgttt
    33601 tgtttcgatt tacttttgat gctgtattta taagattgag ttatgatatt ttacaataag
    33661 tatctttctt tgattgcagg tttcggtttt ttaatagtag gcattctatc ttattttaga
    33721 gtttttactg atgcttccaa aaacgcaggc attgcatcaa ttatcatagg tattatttct
    33781 attatttatc atttccggtt attgcaaaaa aacaagaaag gttaagcgta gtttagtgtt
    33841 agtttaattt atccaacaag aaggaatata ctttaatgtc atagtttaat ttcctttatg
    33901 acctttttat taagtttgtg gaaggaataa tttctaatat tattccagca acgactgatc
    33961 atgaaagcat ttcatttttt aaagtataat gaaagtacat caatagacaa attgattgcg
    34021 cagtcggcac cggaaactgt tttatgtttt gaccttgaag acagtattca ggattgttta
    34081 gactccggaa agacaacagt cttaaaaaaa atatttagag aatatttcag aagtatgctt
    34141 gttgcatgca aaacagatga tggtaagttg aatattggtg tacgcataaa ttcaacagaa
    34201 ggcatggaat ataatttaga ccttttgtca ctttcaggaa ctggacaaat ttcaacagtg
    34261 tttcttccaa aagttgagag tgcgcaacaa attttgaaat tgcaaaatga tttacaggaa
    34321 aacaacataa gctttgatga aataattcct gttatagaaa ccaaagttgg tttgaaaaat
    34381 ctttcatcaa ttttaaagag tgaccttaaa aaagtaaagc gttttgcttt tggtcattgt
    34441 gattataata atgattgtgg taattttcct ttttttcatc aagactcaag agagtactgg
    34501 acatggataa ccaaaatttt taaagaatta aaattgtacg gttataccat tgtgaattct
    34561 ccatgtttac aacttgataa tgaagtgtat tttttgaata tgcttaatat tttagtttca
    34621 atatgtggtt cagactgtgc acaggttact ttaacaaaaa cgcagaccga agtttgtgtt
    34681 tcatattccg taataaaacc tgatgctgag ctacctttaa tgggaaatag attagatttc
    34741 aaagtgccag cagtttatat tgaaagtttt attaaggaat ttgaaaataa gtatcatgaa
    34801 aaaggctttg ttataagtaa tggcagagta ttaatttctc cacaagagta tgttgctgca
    34861 ttaaactatc gcaaaaaggc taacttacct gaaataaatt tcacatttct tggcggttgt
    34921 tttcctgtgc agggaaatat gccatttgaa aatcgtttcc atcaattgct aaaagataag
    34981 gttgagaatt gcagaaatgt aaagttcaat atcaatataa taaggtatga acgatttaaa
    35041 aattgtttta ataagataga aacttataat aagtcgcatc caattgatgt acttgttgtt
    35101 tctataaggc ctgaaccggt gctgcgtttg attaagttat attacaagtt taagaaaaat
    35161 ccaggtggaa gaattgaaag atcattgaat attcctttat tgaataaaat tgatgcagaa
    35221 agttatgatt tactttctgc atctacaaga tttaatccgg tggagaatac agcaaactct
    35281 aagatgcata gcgcattggt tgatttaaat tacttctttg gtatgcttat cggaaattat
    35341 agttatgctt tagaaaagta taaagagctt ttgaaagata ttataaaatt caccaataat
    35401 agaaatatac aactcattgt attaggtccg cccgaaagaa caaatgcact attcgaaaaa
    35461 ctgattacat taagattgga aaaggatata aaaaaatatc tggaatatat tcctgagtgt
    35521 tttgtaagtg gaagaagtca aaattataaa ggcgaaattt tgtttgatag ccttggtgtt
    35581 tatgcaacaa aagcatatca tttgatgatt gctcaagcac tatatgaaaa aatattgcct
    35641 aatattgatg acatcaatat tcgcaaataa ccaactgcgc accatttgat acctgccagc
    35701 ttaatgtaat ttattaacaa cataaaaacc tctctggcgc aagtcttcgg acttgtgccc
    35761 atctatcatt caagcattag t
//
LOCUS       DAOOOL010000010        31670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_106_length_31670_cov_88.734841, whole genome shotgun sequence.
ACCESSION   DAOOOL010000010 DAOOOL010000000
VERSION     DAOOOL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 31670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31670
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106_length_31670_cov_88.734841"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="PKU82_01780"
     CDS             complement(<1..79)
                     /locus_tag="PKU82_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOZ81632.1"
                     /translation="MAKVISIANQKGGVGKTTSAINLAAS"
     gene            complement(109..789)
                     /locus_tag="PKU82_01785"
     CDS             complement(109..789)
                     /locus_tag="PKU82_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOZ81633.1"
                     /translation="MKITYYGHSCFLLEVASKRILFDPFITGNPLAVSIKIQSIKADY
                     ILISHGHGDHIGDAVEIARQTNALVISNFEICNWIQDKGINQVHSMNIGGHKLFDFGK
                     LKCVNAIHSSSFPDGSYGGNPMGFLLETAEGNLYYSGDTALTYDMKLIGDYKRIDLCM
                     LPIGNNFTMGIDNALIASEFVKCDRIIGMHYDTFEPIKIDHAEAKDKFSNAGKELILM
                     NIGSTMTF"
     gene            1011..1718
                     /locus_tag="PKU82_01790"
     CDS             1011..1718
                     /locus_tag="PKU82_01790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ81634.1"
                     /translation="MNKFNVTVFSFLAMAFSACGQKNTGGSQAITTTLDSVSYGIGVS
                     IGENMKKDGLTDMNVDLISRGISEALKGEKTALTMEQVQACIGAYMGEKQKVKDQEAK
                     AKAGVNLEKGKKFLEENGKRKGVVTLPSGLQYEIIKEGTGPKPAATDKVTTHYHGTTI
                     DGKVFDSSVERGQPAQFGVNQVIPGWTEALQLMPVGSKWKLFIPANLAYGESGAGGAI
                     GPNETLVFEVELISIDK"
     gene            complement(1767..3710)
                     /locus_tag="PKU82_01795"
     CDS             complement(1767..3710)
                     /locus_tag="PKU82_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81635.1"
                     /translation="MTKNKKQKESIAEAHQKKQQQKADEARNHKIKILRRILALICGL
                     CGVILYLNTLHHGFVLDDFSVILENRITKQGLKAIPQIFSTSYRSGYYLGTDELYRPI
                     PKSIFAISWNWFGNNPLPLHLLNIVLFGITGIVLFNFCYLLFPNLVFSFIASMLFMFH
                     PVHTEVVANIKSVDEILSFLLCISACIFLARYVQTKRISELVLTSVCFFAALLSKESS
                     ITFLAVFPLLILLKSDKKETSYNWSVELKPMLTLTAFALLFLMIRHSVLSKIASGDPS
                     IADNLLMAAKSSDQKFATAVMILGMYLKLLFIPHPLVFDYSYNQIPLVGMGNIWFLLS
                     LLIHVGLLVWAILLLKKNKLLSFIILFYFISMSIYSNIFYVIGSSFGERFLYTASFGF
                     CMAVAYAFSLLSKNQNVETVSSFFKNNLTVFLIFLPVAFAFGYKTITRNTVWKSNYTL
                     YANDVNLSPNSTRTHYYYGNLLAKDDFQKDKTDQEKDSIQKLALSELEKSVAIFPFGD
                     AYNQMGIIYDKRKEFKKSFESYQQAMKAAPNDPMIQNNLGSLYFNMGQIPEAKACYEK
                     AVQLNPSYSEALMNLGSACGMMKQYDDAVTNYLKAIKFDPNLAQAYYYLGLTYRFKGD
                     QQNSEFYYNQAAKMDPEKYGKKK"
     gene            complement(3713..4243)
                     /locus_tag="PKU82_01800"
     CDS             complement(3713..4243)
                     /locus_tag="PKU82_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81636.1"
                     /translation="MSIRTIIFSALSCILLASCTNTKQQLKDEIDSKEKLLYENKNGR
                     INTKLAREVIIHYRYYAEKFPDDSLSVEYLFKAGDVSVGIAAYDNALEYFKIITEKYP
                     KSNRAAYSLFLQGYISENNLEDTLNARKYYNEFLEKYPNNEMAESARFSILNLGKSAE
                     DVMKQIDGNKDSISEK"
     gene            complement(4254..5060)
                     /locus_tag="PKU82_01805"
     CDS             complement(4254..5060)
                     /locus_tag="PKU82_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ81637.1"
                     /translation="MKNKVVIITGATSGIGNACARYFAQQGCHIVIAARNEEKLNRIG
                     NELKSLYGNCLSIKTDVALEDDCKNLIQKTIDTYNRIDILINNAGISMRALLNDVSLN
                     VMHQVMDINFWGTVYCTKYALPYLLKSQGSVVGVSSIAGKIGLPARCAYSASKFAMEG
                     FLESLRIENLKNNLHVLVAAPGYTASNIRNTALNKDGQQQEESPLDESKLMKPETVAY
                     EIYNAVLHRKRDLVLTTNGKLTVWLNKFFPSWLDKIVYNFVAKEPDSPFK"
     gene            5112..6275
                     /locus_tag="PKU82_01810"
     CDS             5112..6275
                     /locus_tag="PKU82_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81638.1"
                     /translation="MQVIEVNTAAHKKDFLLLPKQIYASDANWIQPLDNDIESVFDAT
                     KNKFFRHGECTRWILRNSSNKVVGRIAAFIDKKTATKNNKQPTGGMGFFECIDDKEVA
                     FKLFDTAKEWLISRGMEAMDGPINFGERDSWWGLLTDGFHEPPYKMNYNPTYYQEFFE
                     SYGFEIYFKQYCYGLKVRNQLDEKFYERHALIKANKDFKAVHIKKSQLEKFAEDFRTI
                     YNKAWVKHGSGKELESKQVQQFFKKMKPVIDEKIIWYVYYKNEPVAFWVNLPDINQIF
                     KYFNGKFGWFEKLRFMLMLKRKVSKRMIGLVFGVIPEFQGKGIDSFMIVDGTAHIMNE
                     TKYEDCELQWIGDFNPKMVSVAESLGSWRSRTLITYRYLFDRNKPFERHPIFA"
     gene            6365..6437
                     /locus_tag="PKU82_01815"
     tRNA            6365..6437
                     /locus_tag="PKU82_01815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6398..6400,aa:Lys,seq:ttt)
     gene            complement(6501..6794)
                     /locus_tag="PKU82_01820"
     CDS             complement(6501..6794)
                     /locus_tag="PKU82_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOZ81639.1"
                     /translation="MFRKTNEESLKEVIEQLLETYKLRDKLNQVKLQRSWDEIMGEMI
                     SKRTEKLILKDHTLYIYLNSAPLKEELSYGREKIVQMLNTALEGDFIKEVVIR"
     gene            6899..7636
                     /locus_tag="PKU82_01825"
     CDS             6899..7636
                     /locus_tag="PKU82_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81640.1"
                     /translation="MQLRLTVIFFLLSFGVIAQDVRYDYVEDLPLRYTNEYSIGATLH
                     SSGWGFMFRKGKNLTVSRKRMYEIEIVSMKHPKEYKSVSYLSERPKTFVFGKMNSLLV
                     MHGGFCYERVLFDKAERRHFEIRWNSTGGPSLGFTKPVYLIFATGNGQRVVEKYDPNN
                     QNHDRQSIYGKAEFTKGLDELKLYPGLFLKSGVMFEYNIMNDGITAVEVGVMLDAYTK
                     TIPIMAFAKNKQVFFNLYGTFMLGRKK"
     gene            complement(7637..8461)
                     /gene="ispE"
                     /locus_tag="PKU82_01830"
     CDS             complement(7637..8461)
                     /gene="ispE"
                     /locus_tag="PKU82_01830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOZ81641.1"
                     /translation="MLNFPNAKINLGLFITSRFDDGYHALETLFYPINWCDILEITEN
                     KNYSKSQPKVNLYISGLAVDGPTDQNLCSKAYHLLKEKFNLPPVNIFLHKQIPTGAGL
                     GGGSSDGAHTLLLLNKLFDLKLSNEDLSVFALNLGSDCPFFISNKPSFAFSKGEILKP
                     APTVLEGNFIVVIKPSVSVSTAMAYSQVKPDKPNKSLTEILNLPKIQWKDFLKNDFEK
                     TILPQFPQIEKLKTNLYQAGAYYASMSGSGSAVFGLFHDEVKLSFLKDEQHHSAWL"
     gene            8555..9514
                     /locus_tag="PKU82_01835"
     CDS             8555..9514
                     /locus_tag="PKU82_01835"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOZ81642.1"
                     /translation="MSNYLDFEKPIEELYEQLEKTKQIGNKGKIDVNNLLTEIEEKIQ
                     VAKKQIYSNLTPWQRVQLSRHPDRPYSLDYINAITDNTFIELHGDRNVKDDKAMIGGF
                     GTVDGRTIMFVGQQKGHNTKSRQYRNFGMPNPEGYRKALRLFKLAEKFNKPIVTFIDT
                     PGAYPGLEAEERGQGEAIARNLYEMSILKVPVICIIIGEGASGGALGIGVGDKVMMLE
                     NTWYSVISPESCSSILWRNWDNKEKAANALKLTANDMLDLKLIDGIIKEPAGGAHSNP
                     EEIFATVKKEINVQLGALLTVSDADRVNNRIDKFCSMGVVRKN"
     gene            complement(9577..11577)
                     /locus_tag="PKU82_01840"
     CDS             complement(9577..11577)
                     /locus_tag="PKU82_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOZ81643.1"
                     /translation="MTHKHIYDSSGKQLCCTLEDNIYENAGRVDLKENHDHNDHDHTH
                     HESGMFSIYLPGLVSLVLLLAGMHFDYWNTFSWFNGWIRLVWYIIAYIPVGFPVIKEA
                     YIGITKGNIFSEFFLMTIATVGAFAIKEYPEGVAVMLFYAVGELLQTMAVRRAKSNIK
                     TLLDQRPDVATVLRNSNTVSVDANDVGIGEVILLKPGEKLALDGILLSDNGTFNTAAL
                     TGESMPDAKVKGASVLAGMINLNTVVKIKVTAAYEDSKLSKMLELIQNASAQKAPTEL
                     FIRKFAKVYTPIVVGLAVAITLLPYFFAHEYIFRDWFYRALIFLVISCPCALVISIPL
                     GYFGGIGAAGRNGILVKGSNYLDVLASVQHVVMDKTGTITEGVFKVQEALIFPEENRN
                     KILQMVNALEKHSTHPVATAIHNYVSNTSTIELEKVEEISGHGMKAFVNGKELLVGNF
                     KLLSLHNISFNVDPATIKHTVIAIAFDRKFAGYIIIADSIKTDAAAAIQKLKQMHVDV
                     TMLSGDKTTVVNSVAEQTAISHAYGELLPEDKVEKVKSIINQKQTVAFVGDGVNDAPV
                     IALSHVGIAMGGLGSDTTIETADVVIQDDNPIKIPLAINIGKQTKKVVWQNITMAFVI
                     KAIVLILGSGGLATMWEAVFADVGVALLAIINAMRLLKMRFD"
     gene            complement(11625..12056)
                     /locus_tag="PKU82_01845"
     CDS             complement(11625..12056)
                     /locus_tag="PKU82_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOZ81644.1"
                     /translation="MLHSNIQTENILVARNTRPTSMRILVYNFLAKQQAALSITEIEK
                     HFQHADRITIYRTLKTFEEKGIIHSIQEDNITKYTLCGSACSEHTHYDQHLHFYCKSC
                     KQTTCKTDIAIPLNNITGFKIDDITFFARGICEKCINPTLQ"
     gene            complement(12084..12551)
                     /locus_tag="PKU82_01850"
     CDS             complement(12084..12551)
                     /locus_tag="PKU82_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ81645.1"
                     /translation="MSTKPIKRHEAIQSISREHYQGLLFGSKIRMGLKKQIEPDRMAK
                     YAVWFYKEHLIPHFEIEEKYLFPVMSPENEMIQRVLKEHRMLADLCKSELELATNSKL
                     ELLEKTLEAHIRFEERVLFKEIESIATSEQLELINKHHKEEKFVDNLSDPFWK"
     gene            complement(12548..12901)
                     /locus_tag="PKU82_01855"
     CDS             complement(12548..12901)
                     /locus_tag="PKU82_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOZ81646.1"
                     /translation="MSSVKIKRIYEAPDKSDGYRMLIDRLWPRGVKKENAAIDEWNKD
                     IASSSELREWFGHDPEKFKEFTARYTKELHNKNDELKQIAGIAKKQNLTLLYGAKDTE
                     HNQAVVLQKVLSKMI"
     gene            complement(13007..13759)
                     /locus_tag="PKU82_01860"
     CDS             complement(13007..13759)
                     /locus_tag="PKU82_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ81647.1"
                     /translation="MFRYFVCFFALTCFSFLGYANDNHSDFREEQNLYGKIYNAPLGI
                     VGNNKVSSVEELYNQKPLIIALVFTRCYGICNPFLLQLSENIKHLQSNSDFNILVISF
                     DPRDNLQDMTNLSKQYGLENNLQWNFAITDSIDAFINSIGFIPKWDSVIKQFDHDALL
                     VGVNEKGLITKNLLGIRIVEDVHKMISSINNVFTPSYRLPTKSNIFSCFNYDPKTQTN
                     RPGLGLLFVALPAIITFLIVTIIVIKSKRTVS"
     gene            complement(13726..15177)
                     /locus_tag="PKU82_01865"
     CDS             complement(13726..15177)
                     /locus_tag="PKU82_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOZ81648.1"
                     /translation="MNIDSLKLKKISALWIIVILVLFIASIVLGILMRLNQGKVIEQS
                     PLTFYTNMTTHGLTMIGIWFAAGMVAVNYLLQRYVKTSYGVNLFAFILTVIGVVMLWV
                     TTYAGKFSTGWTFLYPLPFRDLGSESWSTPLFLISLTILGVGWLVWSVSLMAQIFKKY
                     TLAQAFAWQHFKSNPTVETPPFILITTVTLVGVIASLIAAVIVIVLYFAEYFSHGDFV
                     NDALLMKNLIYFFGHTIANEMLYLGLAVIYEIFGEVSDRPKWKMTWYISLAWNCAMVF
                     ILTAFFHHLYMDFVQPQALQIVGQLASYLASIPSAAVTVFSIVAAVYGVKVRWSIVNL
                     LFFLGVAGWVIGGLGAVIDATISNNIMLHNTLWVPAHFHTYNAMGNVLFSIGFFYWFS
                     TQYVDKLVLSKLTKYTLTLLIVGGIGFLMAFYVAGANSIPRRYSTYPVELTSGPYLAK
                     VGALFATIYLIAILIFFYNIAKRCFVILSASSR"
     gene            complement(15193..15669)
                     /locus_tag="PKU82_01870"
     CDS             complement(15193..15669)
                     /locus_tag="PKU82_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HOZ81649.1"
                     /translation="MSSLKADNDSTGVKNLLKKRFWFTLILIVVLGIFAAATLPKSPY
                     FTYANETPSTVIHVGTMQFSFFLSTKPIDPKSPSGETIEIPHDQLVEFRVTSFDVNHG
                     FAVYDPSMRLITQVQAMPGYVNNLRYKFTEPGEYHIFCLEFCGTGHPVMQTTFKVI"
     gene            complement(16211..16345)
                     /locus_tag="PKU82_01875"
     CDS             complement(16211..16345)
                     /locus_tag="PKU82_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81650.1"
                     /translation="MSKQPPKINHEIIRERALDRILFILEIALVITLIALCGVIITSL
                     "
     gene            complement(16406..18046)
                     /locus_tag="PKU82_01880"
     CDS             complement(16406..18046)
                     /locus_tag="PKU82_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81651.1"
                     /translation="MKLLYTLALSLFTAHLTFAQFSSDPSQPMPVCNAAHGQSNVISL
                     PDGNDGNYLFWLDNRGSVNTTIYALYGQHLDSGGNPLWANNGKVILDKDTVSIKSFNC
                     VLFDGGILVTWVQGQNSYNDSMFVKKINPSGNDVWAQPTLIESTNFSASILGIETYGI
                     KAIPNDSGAFINYSVVYIGGSSGIKFNRINFNGVKQWAASKTNSLPGYTFFTYPGIQN
                     TFYSLSRGNGMGSGIYIQKYNMQSVPQWANPVDISQGVSGNGLAGNCKFLTDNSGNSY
                     VVWDSYSSADILLTKIDAAGNFAWTPSYKSVVNLANTLQGRSDAVLNNNNLFITWSDN
                     RITGQATAFTQKYNASGAAQWIANGVQLGVINSNYCYPKIAYSDSGSVAIAYIMGAPW
                     ELYVQRVRSNGTLTWSGSGKILASDHSFINYADYTITSDSNGCNAVFWHESSTGDLYG
                     AKVCSDGTLVTVAELLNKEVKIFPNPTADQINIELGSSNLINEIEILDMQGRILKTVI
                     IENGQQYQTLNVNEFSKGVYLIKIKAGNESIIKKVIIK"
     gene            18223..21111
                     /locus_tag="PKU82_01885"
     CDS             18223..21111
                     /locus_tag="PKU82_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81652.1"
                     /translation="MMRFVIFILLTFYPLISVYAQDASRIPVFLNNLQTATTDSARVE
                     AYRDLCFNYSIANTDSGIYYGKKGLEIAQRIKFLKGIGDSYNSLGWCYSRKGDYTKAK
                     KHLTLALQYFEQTNDKCNVAVVLSNIGSTYFNQSLYADALEYYLKSVDLSNACTDAIR
                     KPAGLYSIGIVYNSQGEYNKAIPYFREAAALNEQINDSNKLADCINGIGNAYLGLEMY
                     DSAFHYLNQSSAMYQKLSNISGTAYAAESIGSIYLKQGNFDMALKQFEFARENFEKSG
                     SQNDLCYELTLIGDAYRKYGKINSAIIYMKMALQIADSNRFQTLQLQTLNSLSELYAM
                     KHDYQNAYLTFKQAAEIKDTINETKQQARLDEIKTKFETEQKDKMIEILNRDKAIEKS
                     NAEWQRQLKNIFIVGAVMLFLILLNLYNRFSLKKRAAQQLEQKNIIIAQEKERAERSE
                     KFKQQFLANMSHEIRTPLNAIFGMTELLQETKQDEQSSKYLQVIKQSSESLLVIINDI
                     LDLSKIEAGKMEIEKTPFRFRETINHVYQTFSHKAASKELDLKFSVGENVPELIIADP
                     FRITQVLINLVGNAIKFTERGFVIVSINQVRASQKHGLNYCDLLFEVSDSGIGIPAGQ
                     QEKIFESFQQTYAGNSRKYGGTGLGLTISQSLVQLMGGNIEVSSVENQGTIFSFTIEV
                     EVADALAETNKSEQVPIQLIIDKDAFSVLLIEDNEFNQILVKDALSKKMRDIKLQIVG
                     NGRDAIEILKNQKFNLILMDISMPEMDGYEITQIIRNELKESVGEIPIVALTANVSQH
                     EAEKCIAAGMNDYLSKPFKISELTDVINRFKGDVDEQKIDSRIKVAPIASSVDLAYLR
                     DFTDGDIQEMLRYVDMYLQRIPEVFNEIEISLKGDEEKHVIVLLHSIKPLIASVGLND
                     CVQKISELEKQRETIKKNKLMPVVLLIRRQCMTMVDSLKLWKVNNSAG"
     gene            21144..21893
                     /locus_tag="PKU82_01890"
     CDS             21144..21893
                     /locus_tag="PKU82_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ81653.1"
                     /translation="MKNIKAIVVDDEMFGREQIKLLITKYFPQITLVAEVANAAEALK
                     QIYFHKPDLIFLDVEMPDSNGFDLLEKIQEHDFEIIFVTAFDQYAIKAIKFSALDYLL
                     KPLNKEEFKSAVQKFLDRQKSEYDRKSVINNFIRNIKSEEDKLFRLAVNTSNKTYFLL
                     PDEIIRCEADGNYTKITCGNKEVVLSSKTLKEFDDLLNSYNFIRVHRAHLVNKKYIRS
                     FENENKLVLSDNSAIEVSRRKIAEVRNLFGQ"
     gene            complement(21896..22621)
                     /locus_tag="PKU82_01895"
     CDS             complement(21896..22621)
                     /locus_tag="PKU82_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOZ81654.1"
                     /translation="METRQQKYAERYKDVIGASFSFETAQPELPLYYFVSGFAHPFLP
                     VIKHDGLFLFEWGLIPSWIKDAKGASEIRTKTLNAVGETVFEKPSFRKSIVSNRCLLG
                     VSGFFEWRDVNKKKYPYFIKTNNSELFSLGCIYETWVDKNTGELHNTFSIITTVANPL
                     MEKIHNVKKRMPLIIAEEDERKWVDPSTGIDEIRSLIKPYNESEMTAYTISQAANSAR
                     ANRNVPEILNAVNYPELALLDTI"
     gene            complement(22686..23807)
                     /gene="dnaN"
                     /locus_tag="PKU82_01900"
     CDS             complement(22686..23807)
                     /gene="dnaN"
                     /locus_tag="PKU82_01900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOZ81655.1"
                     /translation="MKLIVSSLVLQKHLQSISGVLTTNSPLPILDNFLFAVNGSELTL
                     SASDLETTITTRLKVESKDKGIIAIPGKILLDILKTFNEQPLTFTINEKNHTIEISSD
                     YGKYKLNGQNGEDFPRIPVLEKSNHIELPSSVLHEAINKTIFATGNDDLRPVMSGVFC
                     QLSQDSTIFVATDAHRLVRYRRTDIKSKEAGSFILPKKPLNLLKNILPQEETRVKVDY
                     NEANAFFSFDNYDLICRLIDGRYPNYEAVIPVDNPNVLTVDRVAFLNSIKRVSIFSNK
                     TTYQVKLAIKGSELAISAEDVDYANEANERLTCSYDGSDMDIAFNARFLADIVSNLDT
                     EEIHLHMSLSNRAGLLTPGKDHTNAAEDILMLIMPVMLG"
     gene            complement(23874..24899)
                     /locus_tag="PKU82_01905"
     CDS             complement(23874..24899)
                     /locus_tag="PKU82_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOZ81656.1"
                     /translation="MNKNRILLLIVVLLLSVTGYYYFNNSDSTIKKELRDFAVADTSS
                     IDKMFLADKKGNKALLERKGPALWTINGKYPARQDAINFLLKTIKQIEVRSPVGKAAL
                     QNVLKELSSTGTKIEIYSKGDKLKTYYVGGPTQDMLGTFMYLENSTVPFVIHIPGFNG
                     YLTPRYFASADEWRSRIIFQYGEGQIASVMVQNNQHPDESFRIKKDDDHYVYYSSLTA
                     TAPAEIFQSQLVAYLAKYQLIGFERPTYDLDVAYRDSLLKTGPVRILEVEGVDGKKDK
                     IEMYLKPVDTGTMTSVDERTGLLREFDPDRMYASWNNDTNLIVVQYHVFDKLFSKPDR
                     IKGFGSL"
     gene            complement(24907..26592)
                     /gene="gldG"
                     /locus_tag="PKU82_01910"
     CDS             complement(24907..26592)
                     /gene="gldG"
                     /locus_tag="PKU82_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOZ81657.1"
                     /translation="MKKKIRSANLYQFLLLLLVIAGLNAAGKFLFWRFDLTTEKRFTL
                     SPTTKEKLKQLKDIVYVKVYLTGDLPAGFQRLSSATRDMLMEMKSYAGSNLEFDFIDP
                     SALADSKQRNELFNQLADKGLQPTNISERNAEGTSERILFPGAILNYLSKEQPLLLLK
                     DRMGASAEEMVNTSIQNLEYEIINAISKISTRKPATIGILHGQGELDKPYLADFYKSV
                     SASYPTHYVQIKNQLNALQDYDCLVIAKPDSAFDEKDKFIIDQFIMRGGKVLWLLDMM
                     SADMDSLARKNEFIAIPKELNLDDMLFRYGVRLNKDLVMDLQSVPIPILTGYVGNRPQ
                     NSLLPWFYYPLVTPTSKHPIVNNLNAIRFAFAGSIDTIVSAHIKKTILLQSSHYCKTV
                     QSPAVVDLNILRKDPDEKEYRNPPVNLAVLLEGSFRSNFANRIPYQIATDSTIGFKAD
                     GKPTSMIVISDGDVIRNDYKRSSNTVYPLGMDRYTGQFYGNKNLLLNCIDYLCDNSGL
                     MALRAKEFKLRLLDKTRFDPDKKMLQIVNVALPVLLIILFAMIKLLMRKKKFS"
     gene            complement(26594..27328)
                     /gene="gldF"
                     /locus_tag="PKU82_01915"
     CDS             complement(26594..27328)
                     /gene="gldF"
                     /locus_tag="PKU82_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOZ81658.1"
                     /translation="MYSLFKKEINSFLNSLIGYISIAVFLIVIGLFLWVFPGNDLNIA
                     DSGYAAVDSLFNITPWVYMFLIPAITMRMFSEEKRTGTLEILLTKPLTEFQIVLAKYL
                     SGLVLVIFSLLPTLVYVVTVYKYAAPVGNIDLGGIAGSYIGLFFLASGFVAIGVFASS
                     LADNQLVAFIIAMFLCLLCYTGFQSVATILATGVVANLIDQLGIVAHYNSMSRGVIDS
                     RDVIYFISLTALFLVFTRLKLESRKW"
     gene            27511..28545
                     /locus_tag="PKU82_01920"
     CDS             27511..28545
                     /locus_tag="PKU82_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOZ81659.1"
                     /translation="MGIYIKSSVCISARNTFGETDLSEHSLQTEKLMAREPVYSMIAP
                     AVLRRMGKAVRMTCGAVSKLNLENHPVNGIIIGTANGGMEDCIRFLNQIVQYDEGTLT
                     PTNFVQSTPNAIASTIGMNHQCRGYNITHVHRGNAFENALLDAFMYLVENKNSTLLLG
                     GIDEISDYNYNIERLGGWYKNEIPVNGLYESKTEGSIAGEGCAAFTVSNNSESALLEI
                     VDVSTLHSTDVDEVLQQLKQFCDKHALNLSETMLVSGHNGDVRNETLHYAVESHFQQS
                     AIIRYKHFFGEFPTVSALALWLIGQAATNSGILPEHFYLRKSESKIKNYLICNHYKGY
                     QHGFILTKTP"
     gene            complement(28553..30370)
                     /locus_tag="PKU82_01925"
     CDS             complement(28553..30370)
                     /locus_tag="PKU82_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOZ81660.1"
                     /translation="MNYLSVENISKSFGDRVLFNNISFGIAKGQKLALVARNGTGKTS
                     LLRILAGLDVPDEGTVTFRNQLKVSYLTQENTLPQLATVADCLTENTHISDALLNEKP
                     HYELTEEEQTHLGKIKSVAGKLGVPFFHQKVNELSGGQQKRLALSKVLIDEADFIIMD
                     EPTNHLDLDMIEWLESFLASTNITMLLVTHDRYFLDEICDEILELEDGKLYRHKGNYS
                     YYLEKKAERDEMESANLQRSKNLFRTELDWMRKQPRARGTKSKSRIDAFHVLEEKTKK
                     KNEQGNVALDINISRIGSKILEIHHISKSYGDLKLIDNFDYSLRKGEKIGIVGSNGAG
                     KSTLLNIIMGKEPCDKGKIVMGETIVPGYFEQQSKNISDQKRVIEVIREIADFIPMSG
                     GRKISASQLLERFLFDPSMHFQYVEKLSGGEKRRLYLLTVLMKNPNLLILDEPTNDLD
                     IQTLSVLEDYLASFEGCVIIVTHDRFFMDRVADHIWAFEGDGKISVLNGNYYDYRQML
                     KEQKREQKKEAAAVVRTKPEETTANKKRKLSFKEKQEYEKLENDIMLLEEEKTMLEQQ
                     MGEAELTHEKLMEVSNRFQQVLHDIDAKTMRWMELAEIG"
     gene            30592..>31670
                     /locus_tag="PKU82_01930"
     CDS             30592..>31670
                     /locus_tag="PKU82_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HOZ81661.1"
                     /translation="MPFKKSIYITTSAILIIVALLQWSFITVKENEYQQQALEAYLSK
                     SSLDFSGELEKLKILAGSNKVNSQQLIAQFKACRLAYKKLEGFTGYYFAYTERDLNGP
                     PIPMVEEEPQSKYVRPPHGLQVIEEIISEKPLNKAQLLDEINHTLGLAAKLATSFKTL
                     HPYDYQLFEIMQLRLVKIYTLGINNFDCQIIKTGIPETKAALFSISNILNISYRNADF
                     SMVNNSIDSAVAFLDRQQIRVNQGAEFDYFTFYRNYVVPVNLSLINARKNILKFDPQN
                     IRALNLDATTLFEKEAYNTYFFTKLQRSNNGDMLPYLGKLLFFDPILSVNNAVSCASC
                     HKPNMAYTDGLPKSYGFLKNDTLPR"
BASE COUNT        10291 a         5617 c         5653 g        10109 t
ORIGIN      
        1 aacttgctgc cagattaata gcagatgttg tttttcccac tcctcctttt tgatttgcaa
       61 tcgaaattac tttagccatt tattttattt tttctattgt atatattttt aaaaagtcat
      121 tgtactgcca atgttcatta aaatgagttc cttaccagca ttactgaatt tgtcctttgc
      181 ttctgcatga tcaattttta tcggctcgaa tgtgtcgtaa tgcattccaa taatgcggtc
      241 gcattttaca aactccgaag caattaaagc attgtcaatt cccatggtaa aattattgcc
      301 gataggaagc atgcacaggt caattctttt ataatctcca ataagtttca tgtcgtaggt
      361 tagagccgta tcaccggaat agtagaggtt tccttctgct gtttctaata aaaatcccat
      421 tggattacca ccataagaac catcagggaa cgaactgctg tggatggcgt ttacacattt
      481 caacttaccg aagtcaaaaa gtttgtgccc accaatattc atactatgta cctgattgat
      541 gcctttgtcc tgaatccaat tacagatttc aaaattactg attaccaaag catttgtttg
      601 gcgtgcaatt tcaacagcat cgccaatgtg atcgccatgt ccatgagaaa tgaggatata
      661 atcagcctta atgctctgaa tcttaatact aacggctaat ggattgccgg taataaatgg
      721 gtcgaacagt atgcgtttgg aggcaacttc gagcagaaaa caagaatgac cgtagtaagt
      781 aattttcatc ttaatcagta caatacttca ctttttagca attcacaaaa atagcttcat
      841 tatggtttgc actttcataa aattataaac aaaatgtcaa gttaacgggc tattcataca
      901 gagttattaa cattcaaaat gtgaatgaaa gaaactcagt tttattttct aaggaatgaa
      961 attgcaaatg atataagtct atatttgcgc ggattttaaa aaagcaaata atgaataaat
     1021 ttaatgtaac agtttttagt tttctggcaa tggcgttcag cgcatgtgga cagaaaaaca
     1081 caggtggatc acaagccatc acaaccactt tagactctgt aagctatggt ataggagttt
     1141 ctattggcga aaacatgaaa aaagacggac taaccgacat gaatgttgac ttgatttcaa
     1201 gaggtatttc cgaagctttg aaaggtgaaa aaaccgcctt aaccatggag caagttcagg
     1261 cttgcattgg agcgtatatg ggcgaaaaac aaaaagtaaa agatcaggaa gcgaaagcaa
     1321 aagccggtgt taacttagaa aaaggtaaaa aattcttaga agagaatggt aaacgcaaag
     1381 gtgtggtaac attgccaagc ggccttcagt atgaaattat taaagaaggt accggtccaa
     1441 aaccagcagc tacagataaa gtaactacac attatcatgg aacaaccatt gatggtaaag
     1501 tatttgatag ttctgttgaa agaggtcaac ctgcgcagtt tggcgttaat caggtaatac
     1561 cgggatggac agaagcattg caattgatgc ctgttggttc taaatggaag ttgtttattc
     1621 ctgcaaactt agcttatggc gaaagtggag caggtggagc cattggtcca aatgaaacat
     1681 tggtttttga agttgaatta atttctattg ataagtaatc gcaatataat aatcaaactt
     1741 aaagctgtct gcaaaggcag ctttttttat ttcttcttac cgtatttttc aggatccatt
     1801 tttgctgcct gattataata gaattcagaa ttttgctgat cgcctttaaa acgataggtt
     1861 aagccgagat aataatatgc ctgcgccaga ttaggatcaa atttaattgc ttttaaatag
     1921 tttgttacag catcatcata ttgtttcatc atgccgcagg cgctgcctaa attcatcaac
     1981 gcttcggagt atgaaggatt caattgaaca gctttttcat aacatgcttt tgcctcaggt
     2041 atctgcccca tattgaaata caatgagcct aaattgtttt gaatcatcgg gtcgttaggt
     2101 gcagccttca ttgcttgttg atagctctcg aatgatttct taaattcttt tcttttatca
     2161 taaataattc ccatttgatt ataggcatcg ccaaacggga aaatagcaac agatttttct
     2221 agttcactca atgcaagttt ttgaatagag tctttttcct gatctgtttt gtctttttga
     2281 aaatcgtctt ttgccaaaag attgccataa taatagtgcg ttctcgtact gttgggagaa
     2341 aggttaacat cattagcata caaagtgtag ttcgacttcc agacagtgtt tctggtgatg
     2401 gttttatagc caaatgcaaa ggcaacagga aggaatatta gaaagacagt caggttgttt
     2461 ttgaaaaaag aagaaacagt ttcaacattc tgatttttac ttaataatga aaatgcataa
     2521 gctacagcca tacaaaagcc aaaacttgca gtgtataaaa aacgttctcc aaacgatgag
     2581 cctatcacat aaaaaatgtt ggaatagatg ctcatcgaaa taaaataaaa cagaataata
     2641 aatgacaaca atttattttt cttcaataat aaaatagccc aaactaataa tccgacatga
     2701 attaatagag aaagaagaaa ccagatgttt cccataccaa ccaatggaat ttgattatag
     2761 gaataatcaa aaacaagggg atgaggaata aaaagtagct ttaaatacat tcctaaaatc
     2821 ataacggcag tagcaaattt ctgatcagaa gattttgcag ccatcaacaa gttgtctgca
     2881 atagatggat caccacttgc tatcttactg agaacagaat gacgaatcat cagaaataac
     2941 agtgcaaatg ctgttagtgt caacatgggt tttaattcaa cactccaatt gtatgatgtt
     3001 tcctttttgt cagacttcaa taaaatcagc aacggaaaaa ctgctaaaaa agtaatagat
     3061 gattcttttg aaagcagggc agcaaaaaaa cagactgaag ttaaaactaa ttcagaaatt
     3121 ctttttgtct gaacatatct tgccagaaaa atacatgcag aaatacatag caaaaaacta
     3181 agtatttcat caacactttt tatgttggca acaacctctg tgtgcacagg gtgaaacata
     3241 aacaacattg atgctatgaa agaaaaaacc agatttggaa aaagtaagta acaaaaatta
     3301 aacaacacga tgcccgtaat tccgaaaaga acaatattca acaaatggag tggaagagga
     3361 ttgttaccaa accaattcca tgaaatggca aagattgatt taggtatagg acgatacagt
     3421 tcatcagtac ctaaataata tccacttctg taggaggtag aaaaaatctg aggtatcgct
     3481 tttaagcctt gcttcgtaat gcggttttca agaattacag aaaaatcatc taatacaaac
     3541 ccatgatgca atgtgtttaa atataatatc acaccacaca atccacaaat taaggcaagt
     3601 atcctgcgaa gaattttaat cttatggttt cttgcttcat cagctttctg ctgttgtttt
     3661 ttctgatgtg cttctgcaat actctctttc tgtttcttgt tttttgtcat aattattttt
     3721 cagaaattga gtctttgttt ccgtcaattt gcttcattac atcttctgca ctttttccaa
     3781 gatttaaaat tgagaaacga gcgctttcag ccatttcatt gttgggatat ttttccaaaa
     3841 actcattata atattttctg gcgtttaatg tgtcttccag attattttca cttatatatc
     3901 cttgcagaaa gagtgagtag gcagctctgt tactttttgg atatttctct gtaatgattt
     3961 tgaaatactc caatgcatta tcataagcag caatacccac agaaacatcg cctgctttaa
     4021 aaagatactc aactgacaaa gaatcgtcag ggaatttttc tgcataatag cggtaatgaa
     4081 taattacttc gcgagccagt tttgtattaa ttcgtccgtt tttattctcg tacaaaagtt
     4141 tttctttaga gtctatttca tcttttaact gctgcttggt attggtgcat gatgcaagta
     4201 aaatacagga tagtgcactg aatataattg ttcttattga cattttaatt tgattattta
     4261 aatggtgagt cgggttcttt agctacgaaa ttataaacta ttttatccag ccagcttgga
     4321 aaaaatttgt taagccatac tgtaagtttg ccattggtgg ttaacacaag gtcacgtttg
     4381 cgatgtaaaa cagcattgta aatttcgtaa gctacagttt cgggtttcat taatttagat
     4441 tcgtccaatg gagattcttc ctgctgctga ccatctttat ttaatgcagt atttcggata
     4501 ttacttgccg tatagcccgg tgctgcaact aaaacatgta aattgttttt taagttttca
     4561 atacgcaaag attctaaaaa gccttccata gcaaatttac ttgcactata agcacaacgt
     4621 gcaggtagtc ctatctttcc ggcaatggat gatacaccca ctacagagcc ttgtgatttt
     4681 aaaagatagg ggagtgcata ttttgtgcaa taaactgtcc cccaaaaatt aatatccatc
     4741 acctgatgca tcacgttcaa tgaaacatca ttcagcagag cacgcataga aattccggca
     4801 ttgttaatca gaatatcaat tcggttgtat gtatctatag ttttttgaat aagattttta
     4861 cagtcatctt ctaaagcaac atcggtctta atggataaac agttgccata aagagatttc
     4921 aactcattgc caatgcggtt aagtttctct tcattgcgtg ctgcaataac gatgtgacat
     4981 ccttgctgtg cgaaatatcg tgcacaggca tttccaattc ctgatgtggc tcctgtaata
     5041 atgacaactt tatttttcaa tgaaaaaaat gtataaaatg ctcatggcaa aaataagtag
     5101 ttttgcagcc tatgcaagtt attgaagtta atactgccgc acataagaaa gattttttat
     5161 tacttccaaa gcagatttat gcttcagatg ctaactggat tcagccatta gacaatgaca
     5221 ttgaatctgt ttttgatgct acaaaaaata aatttttcag acatggggag tgtacccgat
     5281 ggattttaag gaacagtagt aataaagttg ttggacgcat tgcagcattt attgataaaa
     5341 aaactgcgac caagaataat aaacaaccga ccggaggcat gggctttttt gagtgtattg
     5401 atgataaaga agttgcattt aaactttttg atacagcaaa agaatggcta atttcaagag
     5461 gcatggaggc aatggatggc ccgataaatt ttggtgagcg tgacagttgg tggggattgc
     5521 ttactgatgg ttttcatgag ccaccctata aaatgaatta taatccaacc tattatcagg
     5581 aattttttga gtcttatggt tttgaaatat atttcaagca gtattgctat gggttaaaag
     5641 taagaaatca gttagacgaa aagttttacg aacgtcatgc attgataaaa gcaaataagg
     5701 attttaaagc tgttcacatt aagaaatcgc aattagaaaa atttgctgaa gatttcagaa
     5761 caatctataa taaagcctgg gtaaaacatg gtagcggcaa ggagttggaa tcaaagcaag
     5821 tacagcaatt tttcaaaaaa atgaaaccgg taattgatga aaaaataatc tggtatgtgt
     5881 attataaaaa cgagcctgta gcattttggg tcaatctgcc tgatatcaat caaatattca
     5941 aatacttcaa tggcaaattt ggatggtttg aaaagctaag gtttatgttg atgctaaagc
     6001 gaaaagtttc taagagaatg attgggcttg tgtttggcgt aattcctgaa tttcagggca
     6061 agggtattga cagttttatg attgtagatg gtacagctca tattatgaat gagaccaagt
     6121 acgaagattg tgagttacaa tggataggcg atttcaatcc caaaatggta tctgtggcag
     6181 agtcgttagg gtcatggcgc agcaggacat taattactta tagatatctt tttgaccgta
     6241 ataaaccttt tgaaagacat ccgatttttg cataaaaagt cgcttattgt taataaaaaa
     6301 gcaaaaatct tttgttattt aaaacggata acaatacttt tgcgccctcg ttattaaaaa
     6361 tcccgattcc gtagctcagc tggtagagca ttacactttt aatgtagggg tcctgggttc
     6421 gagccccagc gggatcacaa aataaaagct gcttcaaaag aggcagcttt tttatttgtc
     6481 attctaatta gaatttggac ttatctgata acaacctctt taataaaatc accttcgagg
     6541 gcagtgttaa gcatctgaac aattttttca cgaccataac tgagttcctc ttttaaaggt
     6601 gcagaattga gatagatata caatgtatga tctttcagaa taagcttttc tgttctttta
     6661 ctaatcattt cgcccataat ttcatcccaa ctgcgttgca gttttacctg attgagtttg
     6721 tcgcgcagtt tatatgtctc caacagttgc tcaattacct ctttcaggct ttcctcattt
     6781 gtctttctga acatgataca aaattagttt ttcagcagta atttagatgt ttaaaaaata
     6841 atcagcatga taaagccctg atttaacggc tgaaataaaa ataatttatt tttgctcaat
     6901 gcaattaagg ctgacagtaa tattttttct gttatcattt ggtgtaatag cacaagatgt
     6961 gcgttacgat tatgttgagg atttgccctt gcgttatacc aatgaatatt caatcggtgc
     7021 aaccttgcac agcagcggct ggggttttat gtttcgcaaa ggaaaaaatc tgacagtaag
     7081 tcgcaaaagg atgtacgaaa tagaaattgt gagcatgaaa catccgaaag agtacaagtc
     7141 agtcagctat ctttctgaac gaccaaagac ttttgtattt ggtaaaatga attctctttt
     7201 ggtaatgcat ggtggatttt gttatgagcg tgttttgttt gataaagcag aacgcagaca
     7261 tttcgagata agatggaatt caacaggtgg tccatcatta gggtttacaa agcctgtgta
     7321 tttaattttt gcaacaggaa acggtcaacg tgtggttgaa aaatatgacc ctaacaatca
     7381 aaatcatgac agacaaagta tttatggcaa ggcagaattt acaaaaggtt tggatgagtt
     7441 gaaactatat ccaggcttat ttttaaagtc aggtgtgatg tttgaataca acatcatgaa
     7501 cgatggaata actgcagttg aagtaggggt gatgcttgat gcttatacca aaactattcc
     7561 tattatggca tttgccaaaa ataagcaggt gttttttaat ctttatggca cctttatgct
     7621 tggccgcaaa aagtaattaa agccacgctg aatgatgctg ctcatccttc agaaaagaca
     7681 atttaacttc atcatgaaac aatccgaaaa cagcagagcc gctgccactc attgaagcat
     7741 aataagcacc ggcctgatag aggttagttt ttagtttttc tatttgtgga aattgtggta
     7801 aaatagtttt ttcaaaatcg tttttcagaa aatctttcca ttggattttg ggtaagttta
     7861 aaatttctgt aagactctta ttgggcttat caggtttgac ctgactgtat gccatagctg
     7921 ttgatacaga aacagaaggt tttattacaa ctataaaatt accctctaat actgttggtg
     7981 caggttttaa tatttcacct tttgaaaagg caaatgatgg tttgttggag ataaaaaaag
     8041 gacaatcaga acccagattt aaggcaaata cagagagatc ttcattactc agttttaaat
     8101 caaacaattt attcaaaagg agtagtgtgt gtgcaccatc ggatgatccg ccaccaagtc
     8161 cagcaccggt tggtatttgt ttatgcagaa atatatttac aggtggaaga ttgaattttt
     8221 cttttagaag gtgataggct ttgctgcata aattctgatc agtaggccca tcaactgcca
     8281 atcctgatat atataaattt actttaggtt gtgatttgct ataattttta ttctcagtta
     8341 tttctaaaat atcgcaccag ttgataggat agaacaatgt ttccaatgca tgataaccat
     8401 cgtcaaaacg agaggttata aataacccga gattaatttt ggcatttgga aaattaagca
     8461 taagtgccgg caaagataaa ttttcgataa gttttaacaa tcatcagaca taatcaaatt
     8521 atgctacttt tgtcattgca aataaaaaga aaacatgtcc aattatcttg attttgaaaa
     8581 gccaattgaa gagttatacg aacaattgga aaaaacaaaa caaataggca ataaaggaaa
     8641 aatagatgta aacaatcttt taactgagat tgaggaaaaa attcaggtag caaaaaagca
     8701 aatctattca aacctgacac cgtggcaacg tgttcaatta agtcgtcacc cggacagacc
     8761 gtattctctc gattatataa atgctattac cgacaatact tttattgaat tgcatggtga
     8821 cagaaatgtt aaggacgata aagcaatgat aggaggtttt ggtactgttg atggaagaac
     8881 aattatgttt gttggtcagc aaaagggaca caatacaaag tccaggcaat atcgtaactt
     8941 cggtatgccc aacccggaag gttatagaaa agcattacgt ctgtttaaac ttgctgaaaa
     9001 gtttaataag cccattgtta ctttcattga cactccgggt gcctatcccg gacttgaagc
     9061 agaagaacgc ggacagggtg aagctattgc tcgcaatctt tatgaaatgt caattttaaa
     9121 ggtgcctgtt atttgtatta tcattggcga aggtgcatcc ggtggtgcat taggaattgg
     9181 tgtaggcgat aaagtaatga tgcttgaaaa cacatggtac tctgtaattt cgcctgagtc
     9241 atgttccagt attttatggc gcaactggga taataaagaa aaggcagcca atgcgcttaa
     9301 acttactgcc aatgacatgc tcgatttaaa attaattgat ggcattatta aagaaccagc
     9361 aggtggtgca catagtaatc cggaagaaat ttttgcaacc gtaaaaaaag aaattaatgt
     9421 tcagttaggt gccttactta ctgtatctga tgctgacagg gttaacaaca gaattgataa
     9481 attctgttct atgggtgttg ttcgaaagaa ctaaaaacat tttttactga ttaattcttt
     9541 ctaaataggt ttatattatt ggctgattcg ttaatactaa tcgaatctca tttttaataa
     9601 acgcattgca ttgatgattg ctaatagtgc aacaccaaca tctgcaaaca ctgcttccca
     9661 catagttgcc aatccacctg agcctaatat taaaactatt gcttttatta caaacgccat
     9721 agtgatattt tgccagacca ctttctttgt ttgctttcca atatttattg ctaaaggaat
     9781 tttaatcggg ttgtcatcct gaattacaac atctgcagtt tcaatagtag tatcgctgcc
     9841 aagtccaccc attgcaatac caacatgact taatgcaata acgggtgcat cattaacacc
     9901 atcaccaaca aaagctactg tttgtttttg attgattatg gatttaactt tttcaacttt
     9961 atcctcaggc agtaactcac cataagcatg agaaattgct gtttgctcag caactgaatt
    10021 tacaacagtt gttttatcac cactcaacat cgtaacatct acatgcatct gttttaattt
    10081 ttgtatggca gcggcagcat cggttttaat gctatctgca atgatgatgt agcctgcaaa
    10141 ttttctatca aaagcaatgg caatgacggt atgctttatg gtagcaggat caacattaaa
    10201 tgatatgtta tgtaaggata aaagcttgaa atttcctact aacaactctt tgccattaac
    10261 aaatgctttc attccatgac ctgaaatttc ctctaccttt tccagttcaa ttgttgaggt
    10321 atttgaaaca tagttgtgaa tggctgttgc aacaggatgt gtactgtgtt tttcaagcgc
    10381 attaaccatt tgcaaaattt tatttctatt ttcttccgga aaaattaaag cttcctgaac
    10441 tttaaatacg ccttcagtaa ttgttccggt tttgtccatc acaacatgct gcaccgatgc
    10501 aagtacatct aaataattac tgcctttaac caaaatacca ttccggccgg cagcgccaat
    10561 gcctccaaag tatcctaaag gaatacttat taccaatgca caaggacatg atatgactaa
    10621 aaatattaaa gctctgtaaa accaatcacg aaaaatatac tcatgagcaa aaaagtaggg
    10681 caataaagtg atggcaacag caagtccaac aacaataggg gtataaactt ttgcaaattt
    10741 tctgatgaat aattcagtgg gagctttctg tgcagatgca ttttgaatta attcaagcat
    10801 cttgctcaac ttgctatctt cataagcggc tgtgacttta atttttacaa cagtattaag
    10861 gttaatcatg cctgcaagaa ctgaagctcc tttcactttt gcatcgggca tactctctcc
    10921 tgttagtgca gcagtgttaa aagttccgtt atcagaaagt aaaataccat caagtgctaa
    10981 cttttcgcct ggttttaaaa gtattacttc gccaatgcca acatcgttag catctacaga
    11041 aacagtgtta ctatttcgta atactgttgc aacatcagga cgttgatcaa gtaaggtttt
    11101 aatatttgat tttgctcttc ttacagccat tgtctgaagt aattctccca cagcatagaa
    11161 taacattaca gcaacacctt caggatattc ttttattgca aatgcaccaa cggtagctat
    11221 tgtcatcaga aaaaactcag aaaaaatatt tccttttgta attccaatat aagcttcctt
    11281 gattacagga aatccaaccg ggatataggc tattatatac caaactaaac gaatccaccc
    11341 attaaaccag gagaaggtat tccaataatc aaaatgcatt cctgccagaa gaagcactaa
    11401 agaaactaat ccgggcagat atattgaaaa cataccggac tcatgatgcg tatggtcatg
    11461 atcattgtga tcgtgatttt ctttcaaatc aacacgacct gcattttcat aaatattatc
    11521 ttcaagagta caacaaagtt gttttccgct ggagtcgtaa atgtgtttat gcgtcatgcc
    11581 atatattttc tgaatacaaa attatggcag atgtcaatgc aatcctattg caaagtcggg
    11641 ttaatgcatt tttcgcaaat acctcttgca aaaaatgtta tatcgtcaat tttaaatccg
    11701 gtaatgttgt ttagcggaat ggcaatatcg gttttgcagg tagtttgttt gcaagactta
    11761 caataaaagt gaagatgttg atcgtagtgg gtatgttctg aacaagcaga tccacataaa
    11821 gtatatttgg ttatattatc ttcctgaatg ctatgtataa tgcctttctc ctcaaatgtt
    11881 tttaaagtac gatagattgt aattctgtca gcatgctgaa aatgtttctc aatctcagtg
    11941 atagataatg cagcttgttg ttttgcaagg aaattgtaaa ctaaaatacg catgcttgtc
    12001 gggcgtgtat ttctggcaac taaaatattt tctgtttgta tgtttgagtg caacatgttg
    12061 ttcattgaaa ttatgagtag gtgttacttc cagaatgggt cagataaatt gtcaacaaac
    12121 ttttcctctt tatgatgttt gtttatcagt tcgagctgtt cagatgtagc aatagattca
    12181 atttctttaa agagtactct ctcttcaaag cgaatgtggg cttccaatgt tttttcgagt
    12241 aattcaagtt tgctgtttgt agccaattcc aattctgatt tacataaatc agcaagcatg
    12301 cgatgttctt taagtactct ttgaatcatt tcattttcag ggctcataac cggaaataaa
    12361 tatttctctt ctatttcaaa gtgaggaatg agatgttctt tataaaacca aacagcgtac
    12421 tttgccattc ggtcaggctc tatttgtttt ttaagcccca tcctgatttt agaaccaaaa
    12481 agtaatcctt gataatgttc tctgcttatg gactgtattg cctcatgtcg cttaattggt
    12541 ttggtgctca tatcatttta ctcaatactt tttgtaaaac tacagcttgg ttgtgctctg
    12601 tatcttttgc gccataaagt aacgtcaagt tttgcttttt tgcaattccg gctatttgct
    12661 ttaattcatc attcttgttg tgaagttctt ttgtataacg agctgtaaat tctttgaatt
    12721 tttcagggtc gtgaccaaac cattctctta gctctgaaga tgatgcaata tctttattcc
    12781 attcatcaat ggcagcattt tcttttttaa cacctcttgg ccatagcctg tcaatcaaca
    12841 ttctataacc atcacttttg tccggtgctt cataaatccg ttttattttt actgatgaca
    12901 taactgtttt aatttttaaa cttcatatca gaaaacaaaa ttgcattgat tatggtttta
    12961 tagttccgat aatttatttt aagtgacaaa aagtgtgttt aaaattctaa gaaactgttc
    13021 tctttgattt aataacaatt attgtaacaa taagaaaagt tatgattgca ggcaaggcta
    13081 caaataaaag tcctaagcca ggtctgtttg tttgtgtttt cggatcgtag ttaaagcatg
    13141 aaaatatatt actttttgtt ggtaaacggt aggagggagt aaatacatta ttaatgctgc
    13201 ttatcatttt atgtacgtct tccacaattc tgattcccaa aagattttta gttataagcc
    13261 ctttctcatt tacaccaacc agcaaagcat cgtggtcaaa ctgtttgata acgctatccc
    13321 atttcggaat aaatcctatg gaattaataa atgcatcaat actatcggtt attgcaaagt
    13381 tccattgaag attattctca aggccatatt gctttgaaag attagtcata tcctgaaggt
    13441 tatctcttgg atcaaaactt ataactaaaa tattaaaatc tgaattgctt tgtagatgct
    13501 ttatattttc gctcaattga agtaagaatg gattgcagat gccataacat cttgtaaaaa
    13561 ctaaagctat gattaatggt ttctgattat ataattcttc aacagaagat actttattat
    13621 taccaacaat tccgagtggt gcgttataaa tttttccgta aagattctgc tcttccctga
    13681 agtcagagtg attatcatta gcataaccta aaaagctaaa acatgttagc gcgaagaagc
    13741 agacaaaata acgaaacatc gtttagcaat attataaaag aatatcaaaa tagcgataag
    13801 atatattgtt gcaaacaaag cacctacctt agcaagataa ggccctgatg tgagttcaac
    13861 aggatatgta ctatatcttc ttggaattga atttgcaccg gccacataga atgccattaa
    13921 gaagccaatg ccaccgacaa ttagaagtgt taaagtgtat tttgtaagct tacttaaaac
    13981 taatttgtca acatattgcg tagagaacca gtaaaagaaa ccgatgctaa acaaaacgtt
    14041 tcccatggca ttgtaggtat gaaagtgtgc aggaacccaa agcgtattat gcagcataat
    14101 attatttgaa atagttgcat caatgacagc ccctaagcca ccaattaccc atccggcaac
    14161 accaagaaag aacaataggt ttacaataga ccatcttacc ttaactccat aaactgctgc
    14221 tacaatactg aaaaccgtca cagcagcaga tggaatactt gcaagatatg aggccaattg
    14281 tccaacaatt tgcaaagcct gtggttgcac aaagtccatg tataggtgat gaaaaaatgc
    14341 agttaaaata aataccattg cacaattcca tgccaatgaa atataccagg tcattttcca
    14401 ttttggcctg tcgcttactt ctccaaaaat ttcatagata acagccaagc ctaaataaag
    14461 catttcgttt gcaatggtat gaccaaagaa atagatcagg tttttcatca gcaaagcatc
    14521 attgacaaaa tctccatgag agaaatattc tgcgaaatac aatacaataa caattactgc
    14581 tgcaattaac gaggcaatga caccaactaa tgtcactgtt gttatcaata taaaaggtgg
    14641 tgtctctaca gttggattac ttttgaaatg ttgccatgca aaggcttggg ccaatgtata
    14701 ttttttaaaa atctgagcca tcagactaac actccacacc agccatccta cacctaatat
    14761 tgttaacgaa ataagaaaca acggtgttga ccaactctcc gaaccgagat ctctgaatgg
    14821 taatggatag agaaaagtcc agcctgtgct aaacttacct gcataggtag ttacccaaag
    14881 cattacaaca ccaataacag taagaataaa ggcaaacaag ttaacaccat aacttgtctt
    14941 cacataccgt tgtaaaagat agtttacggc aaccattcct gcagcaaacc aaatcccaat
    15001 catggttaaa ccatgtgttg tcatgtttgt atagaaagta agtggcgatt gttcaataac
    15061 ttttccctga ttgagcctca taagaattcc aagtacaata cttgcaataa aaagtacaag
    15121 aatgacaatt atccaaagtg cgcttatttt tttaagctta agtgaatcta tattcatgat
    15181 ttaatttttt tattaaataa ctttaaatgt tgtttgcata acgggatgcc ctgtgccaca
    15241 aaactcaagg caaaaaatgt gatactcgcc aggttcagta aatttatagc gtaggttgtt
    15301 gacatagccg ggcattgcct gtacctgagt aatcaatcgc attgaaggat cgtaaacagc
    15361 aaatccatga ttgacatcaa aacttgttac tctgaattct acgagttggt cgtgcgggat
    15421 ttcaattgtt tctccacttg gacttttagg gtcaatgggt ttagtggaaa gaaaaaatga
    15481 aaattgcatt gtgccaacat gaatcacagt tgaaggtgtt tcattcgcat aagtgaagta
    15541 tggggatttt ggtaaagttg ctgcagcaaa aatgccaagt acaacaatga gaattaaggt
    15601 aaaccaaaaa cgttttttta acaggttctt tacacctgtt gaatcattgt cagctttgag
    15661 tgaagacata aagattgaca caaaaaatat agtgccaata ccaacaagca gcataaaaaa
    15721 taacataatg gaatgttgtt gcataacttt tagtttaaat aattaaagtg aatgtgatgt
    15781 atatgatgat taagattaac ttgtataggt ttgttaatcc gaattcctga atgaataaat
    15841 tgagtagaga aaagttgtag atttttgaag aatctatgac attttttacc tcttaaaatg
    15901 gggcaaagca ttgccatcaa gccagcggaa ttttggttgc ctccaaaaac aatttcccgt
    15961 tgatgtaata taaccttttt atgggttaca ggtgcataaa atataaaaca tgctaccatg
    16021 tatccgttaa actacattag gtagtgcaaa cgtataaact atttcttagg aatccaaaag
    16081 aagtatataa atacttttat aacatttatg tttggcacat gtagcttaaa ccttaaatcg
    16141 taatttaaca taagtttctc ttaaaatctc gtaatgattt cttgtagaat ttaacttata
    16201 cattaatttc tcaaagtgaa gtaataatta caccacataa ggctattaag gtaataacca
    16261 aagcaatttc aagaataaat aaaattcgat ctaatgccct ttcacgaatt atttcatgat
    16321 taatttttgg aggttgtttt gacatggacg ttattatatt taaaactccg gctttatgcc
    16381 ggagttttgc tgttttggtt aaaggctatt taataataac ttttttgatg atactttcat
    16441 tacctgcttt tatctttatc agataaacac cttttgaaaa ttcatttaca ttaagcgttt
    16501 ggtactgctg tccgttttca ataatcacag tttttaatat tctcccctgc atatcaagaa
    16561 tttcaatttc attaatcagg ttcgatgagc ccaactcaat attaatttga tctgctgtcg
    16621 gattcggaaa tattttaact tctttattta atagttctgc aacagttaca agcgtaccat
    16681 cactgcaaac ttttgcacca tataaatctc ctgtacttga ttcatgccaa aaaacagcat
    16741 tacatccatt tgaatcagaa gtgatggtat agtctgcata gttgatgaaa ctatggtcac
    16801 ttgcaagtat tttaccactg ccgctccaag ttaaagtacc attgcttcga acacgttgta
    16861 catataattc ccaaggtgct cccataatat atgctatggc tacagagcca ctgtcactat
    16921 atgctatttt tggataacaa taattggagt tgattacccc taattgtact ccattggcta
    16981 tccactgtgc agcaccgctt gcattatact tttgtgtaaa agctgtagcc tgaccagtaa
    17041 ttctgttatc gctccaagtg ataaacaagt tgttgttatt taaaacagca tctgatctgc
    17101 cttgtaaagt atttgccaag ttaacaactg atttatatga tggagtccat gcaaaattac
    17161 cggcagcatc aatcttagtc agaaggatat cggcagatga ataactatcc caaactacat
    17221 aagaatttcc tgagttatct gtcaggaatt tgcaattccc tgcaagtcca tttcctgaaa
    17281 caccttgaga aatatctacc ggatttgccc attgtggtac actttgcata ttatattttt
    17341 gaatataaat tcctgatccc attccatttc ctctggataa tgaatagaaa gtgttttgta
    17401 ttcccggata agtaaagaaa gtatagccgg gcaaggaatt tgtttttgag gcagcccatt
    17461 gttttactcc gttgaaatta atccgattaa atttaattcc gcttgatcca cctatataaa
    17521 ctacactata gttaataaat gcaccggagt cattggggat tgcctttatc ccataagttt
    17581 caattcctaa aattgaagcc gaaaaatttg tcgattcaat taaagttggc tgtgcccaaa
    17641 catcattgcc tgaagggttg attttcttta caaacattga atcattatat gaattctgac
    17701 cttgtaccca agtaactaaa ataccaccat caaacaatac acaattgaat gattttattg
    17761 aaacagtatc tttatcaaga atcactttac cattgtttgc ccataaagga tttccaccgg
    17821 aatctaaatg ctgaccatag agcgcataaa ttgttgtatt aacagatcct ctgttgtcga
    17881 gccagaaaag ataatttcca tcattaccat caggaagact aatcacgtta gattgtccat
    17941 gtgcagcatt gcagaccggc attggttgtg agggatcgct actgaattga gcaaatgtta
    18001 aatgtgcggt aaaaagtgaa agtgctaagg tgtaaaggag tttcatattt tttgttttta
    18061 atagttaaac atattttttg aaagaattta taatccaata ttagttgcta tatgatatac
    18121 atattgcatt aacttattga atggttgtgt ttaattatta aacggtaagt attctattaa
    18181 aagttttctt actttggtaa ctcatttggc aactaattat agatgatgcg ttttgttatt
    18241 tttattttac tgacttttta tccgttaatt tctgtttatg cgcaggatgc atctagaatt
    18301 cctgtttttt taaataatct tcaaacggct actactgatt ctgcaagagt ggaagcctat
    18361 cgtgatttat gttttaatta cagtattgca aatacggaca gtggaatata ctatggtaaa
    18421 aagggacttg agattgcaca aagaatcaag tttttaaagg gcattggtga tagttataat
    18481 agcttaggtt ggtgttattc tcgtaaggga gattatacta aagcaaagaa gcatcttaca
    18541 ttagctcttc aatattttga acagaccaat gataaatgta atgtggcagt agtattatcc
    18601 aatatcggca gcacatactt taatcagagt ttatatgctg atgccttaga gtattatctg
    18661 aagagtgttg atttatccaa tgcatgtact gatgctattc gaaagccggc aggattatat
    18721 tctataggca ttgtgtataa cagccaagga gaatataata aggccattcc ttattttaga
    18781 gaagctgcag cactaaatga gcagataaat gatagtaata aactggctga ttgcataaat
    18841 ggaattggta atgcatatct tggtttagaa atgtatgata gtgcatttca ttacctgaat
    18901 caatcttctg ctatgtatca aaagttatct aacataagtg gaacggccta tgcagcagag
    18961 agtattggaa gtatttatct caaacaagga aattttgata tggcattgaa gcaatttgag
    19021 ttcgccagag agaattttga aaagtcaggc agccagaatg atttatgtta tgaacttacc
    19081 ttgataggag atgcttacag gaaatatggt aaaataaatt ctgcaatcat ttatatgaaa
    19141 atggctttgc agatagcaga tagcaataga tttcaaactt tacaattaca gacactaaat
    19201 tcgttaagtg agttgtatgc aatgaaacat gattatcaga atgcatatct gacttttaag
    19261 caagcagcgg aaattaaaga cactatcaat gaaacaaagc agcaagcccg acttgatgag
    19321 ataaaaacaa aattcgaaac tgagcagaaa gataaaatga ttgaaatact caatagagat
    19381 aaggctattg agaaatcgaa tgccgaatgg caacgacaac taaagaatat ttttattgta
    19441 ggagcagtga tgctatttct catcttgtta aatctgtata accgtttttc attaaagaaa
    19501 agggcagcac agcagctaga gcagaaaaac atcatcattg cacaggagaa agagcgtgct
    19561 gaacgcagtg aaaaatttaa gcaacaattt ctggctaata tgagtcatga aatcagaacg
    19621 ccattaaatg ccattttcgg aatgactgaa ttgttgcagg aaactaagca agatgagcaa
    19681 agtagtaagt atttacaagt cattaaacaa tcgtcagaaa gtttgttggt cattattaat
    19741 gatattcttg atttatcaaa aatagaagcc ggtaaaatgg agattgaaaa aactccattc
    19801 agattcagag aaactattaa tcatgtttat cagacttttt ctcataaagc tgcgagtaaa
    19861 gaactcgatt tgaaattttc tgtaggagaa aatgtgcctg aattaatcat agcagatccc
    19921 tttcgtatca cacaagtact tataaattta gttggaaatg caattaaatt tactgaacgt
    19981 ggatttgtta ttgtttcaat taatcaagta agagcatcac aaaagcatgg actaaattat
    20041 tgtgatcttc tttttgaagt atcggatagt ggtattggaa tacctgctgg tcagcaagaa
    20101 aaaatatttg aatcttttca gcaaacatat gcaggaaatt ctagaaaata tggtggtaca
    20161 ggtcttggtt tgacgatttc gcaaagtctt gttcagttaa tgggtgggaa tatagaggtt
    20221 agtagtgtag aaaatcaggg cacaatattc tcctttacaa tagaagtaga agttgcagat
    20281 gctttagcag agacgaataa atctgagcaa gttcccattc agttgataat tgataaagat
    20341 gcgttttctg ttttgctgat tgaagataat gagtttaatc agatactagt taaagatgca
    20401 ctttcaaaga aaatgcgtga tataaaattg caaattgttg gaaatggaag ggatgcaata
    20461 gaaatactga aaaaccaaaa gttcaacctg attttaatgg acatttcaat gcctgaaatg
    20521 gatgggtatg aaattactca gattattcgg aatgagctta aagaatcagt tggagaaatt
    20581 ccgatagttg cactcactgc aaatgtatcg caacatgaag cagagaaatg tattgccgca
    20641 ggtatgaacg actatctttc aaaacctttt aaaatttctg aactgacgga tgtaataaac
    20701 cggtttaaag gtgatgttga tgaacagaaa attgacagca gaattaaggt tgcgccaatt
    20761 gcatcttcag ttgatttagc atatctgaga gactttactg atggcgatat tcaggagatg
    20821 ttgcgttatg ttgatatgta tctccaaaga atccctgagg tttttaatga gattgaaatt
    20881 tctttaaaag gagatgaaga gaaacatgta attgtcttat tacacagcat taaacctctc
    20941 attgcttctg ttggtttgaa tgactgtgtg caaaaaattt cagaactcga aaagcagaga
    21001 gaaactataa agaaaaacaa gttaatgcct gttgtattac ttataagaag gcaatgtatg
    21061 acaatggttg attctttaaa gctatggaaa gtaaataatt ctgccggcta aaaatatatt
    21121 tgtacaataa ttacattagt gaggtgaaaa atataaaggc aattgtggtt gatgatgaaa
    21181 tgttcggtag agagcagata aaattattga taacaaaata cttccctcaa attacattag
    21241 ttgctgaagt agctaatgca gcagaagcac taaagcagat ttattttcac aaacctgatt
    21301 tgattttttt agatgttgaa atgccagaca gtaatggttt tgatttgctt gagaaaatac
    21361 aggaacatga ctttgaaata atttttgtta cagcttttga ccaatatgca attaaagcaa
    21421 ttaaattttc ggcattagat tatttgctaa aacctctgaa taaagaagaa tttaaaagtg
    21481 cagtgcagaa atttctcgac agacagaaaa gcgagtacga cagaaaatct gtcataaata
    21541 attttatcag gaatattaag tcagaagaag ataagctgtt ccgattggcc gttaacactt
    21601 caaataaaac atattttctc cttcccgatg aaattatcag atgcgaagct gatggaaatt
    21661 atactaagat tacttgtggc aataaagagg ttgttctatc atcaaaaaca ttaaaagagt
    21721 ttgacgattt acttaacagt tataatttca tcagagtaca cagagcgcat ttagtcaaca
    21781 aaaaatatat aaggtcattt gaaaacgaaa acaaattggt gctgagtgat aattctgcca
    21841 tcgaagtttc aagaagaaaa attgcagaag tcagaaattt atttggtcaa taatttcaga
    21901 ttgtatccaa gagtgccaat tccgggtaat ttactgcatt taaaatctcc ggaacattac
    21961 gattagccct tgcactgttt gctgcctgcg aaatggtata ggccgtcatt tcagattcat
    22021 tatatggttt aatcagtgat ctaatttcgt caatacctgt gcttggatcc acccacttcc
    22081 tttcatcttc ttctgcaatt atcaacggca tccttttttt tacgttatgt attttttcca
    22141 tcagtggatt tgccacagta gtgatgatgc tgaaagtatt atgtaattca ccggtgtttt
    22201 tatcaaccca ggtttcataa atgcagccaa gagaaaataa ttcagagttg tttgttttaa
    22261 taaaataagg atatttcttt ttgtttacat cgcgccattc aaaaaagcct gaaacaccaa
    22321 gcaaacaacg attgctaaca atacttttcc tgaatgatgg tttttcgaaa actgtttcac
    22381 caacggcatt gagtgtttta gttcttattt ccgaagcgcc tttagcatct tttatccatg
    22441 agggtatgag tccccattca aataaaaata gtccatcatg tttgattact ggtaaaaacg
    22501 gatgtgcaaa tccactgaca aaatagtaaa gtggtaattc aggttgtgct gtttcaaagc
    22561 tgaatgatgc tccaataaca tccttataac gttcggcata tttttgctgc cgggtttcca
    22621 ttattgcaat attgtaacac ataaaaattt acattcttaa caatataaac cggcaaaaaa
    22681 atttatcagc caagcataac aggcataatg agcatcagaa tatcttctgc tgcattggta
    22741 tggtcttttc cgggagttaa taatccggca cggttcgaca atgacatgtg cagatgaatc
    22801 tcttcggtgt caagatttga aacaatatct gcaagaaaac gcgcattaaa tgcaatgtcc
    22861 atatcagatc catcataaga gcaggttaaa cgctcattgg cttcgttggc ataatctaca
    22921 tcttcagcag aaattgccaa ttcgcttccc tttattgcaa gttttacctg ataagttgtc
    22981 ttgtttgaaa aaatagaaac acgttttatc gagttcagaa atgcaactct gtcaacagtt
    23041 aaaacatttg gattgtcaac aggaattact gcttcgtagt tcggataacg tccatcaatc
    23101 aaacggcaaa tcaaatcata gttgtcaaaa ctgaaaaatg cattggcttc gttatagtca
    23161 acctttacgc gtgtttcttc ctgaggtaaa atatttttca gaaggttcaa cggcttttta
    23221 ggtagaataa atgagcctgc ttcttttgac tttatatctg ttctgcgata gcgcaccaaa
    23281 cgatgtgcat cagtagcaac aaaaatggtg ctgtcctgcg aaagctgaca aaatacacct
    23341 gacattacag ggcgcaagtc atcattgccc gtagcaaaaa tggttttatt tatcgcctca
    23401 tgcaaaacag atgatggcaa ttcaatgtgg ttactttttt cgagaaccgg tatgcgcgga
    23461 aagtcttcac cattctgacc attgagttta tactttccat aatctgagct tatctcaatg
    23521 gtatggtttt tttcattgat ggtaaaagta agcggctgct cattaaaagt ttttaagatg
    23581 tcgagcagaa ttttacccgg gatggcaata atgcctttgt ctttagattc aactttaagt
    23641 cttgtggtta ttgttgtttc aaggtcagaa gctgataaag taagctcact gccattaacg
    23701 gcaaacagaa aattgtctaa aatcggcagg ggagagttag tggttaaaac cccgctgata
    23761 gattgtaaat gtttttgaag tacaaggctg gaaacaatta gtttcataaa tatcaaaagt
    23821 taggtggcaa atataatcag atggaacttt gtgaaggcta aattaaagaa aaattaaaga
    23881 ctaccgaacc ccttgattct gtcgggttta ctaaatagtt tgtcaaatac atgatactga
    23941 accacaatca aattggtgtc gttattccaa gaggcataca ttctgtcagg gtcaaattcg
    24001 cgcaaaaggc ctgttctttc atcaacactg gtcatggttc ccgtgtcaac aggcttcaga
    24061 tacatttcaa ttttatcttt tttgccatca acaccttcaa cttcaagtat tctgacaggc
    24121 cctgtcttta ataacgaatc gcgataggca acatcaaggt cgtaggttgg ccgctcaaat
    24181 cctataagct ggtattttgc taagtatgca actaactgag attgaaaaat ttctgccggt
    24241 gcagttgcag tcaggcttga ataataaaca taatgatcat catctttttt tattctgaaa
    24301 gactcatcgg gatgttggtt attctgaacc ataacagaag caatttgccc ttcgccatat
    24361 tgaaaaataa tgcggctgcg ccattcatcg gcactggcaa aataacgagg tgtaaggtat
    24421 ccgttaaagc ccggaatatg aatcacaaag ggtacggtag aattttcaag atacataaag
    24481 gtgccaagca tatcctgtgt tggtccgcca acgtaataag ttttcagttt atcgcctttg
    24541 ctgtaaatct caatttttgt tcctgtgctg ctcaactctt tcagtacgtt ttgcagggca
    24601 gctttaccaa caggacttcg tacttcaatt tgttttattg ttttgagcaa aaaattaatt
    24661 gcatcctgac gtgcaggata tttaccgtta atagtccaga gggcagggcc ttttctttcc
    24721 agcaaagctt tgtttccttt tttatcagcc agaaacattt tatcaattga agaagtatcg
    24781 gcaactgcaa aatcgcgcag ttctttttta attgtactat cgctgttgtt gaaataataa
    24841 tatccggtta cagaaagcaa caacacaact atgagaagta aaattcggtt tttattcatt
    24901 attctatcag gagaatttct ttttccgcat caacaactta atcattgcga aaagaattat
    24961 taaaagaaca ggcaatgcaa catttactat ttgcagcatt tttttatcag ggtcaaatcg
    25021 tgttttatct aacaggcgca gtttaaattc ttttgctcta agagccatca atccggaatt
    25081 atcgcataaa taatcaatac aatttagcaa tagattttta tttccgtaaa actgtccggt
    25141 ataacgatcc atcccaagcg gataaaccgt attgctgcta cgtttataat cattacggat
    25201 aacatcacca tcagaaatca caatcatgct cgttggttta ccatctgctt taaatcctat
    25261 ggtgctgtct gttgcaatct gatatggaat tctgtttgca aaattagagc ggaatgaacc
    25321 ttcaagcaaa acggcaagat ttacaggtgg gttacgatat tctttttcat caggatcttt
    25381 gcgcaggata tttaaatcta ccactgccgg tgattgaact gtcttgcagt agtgcgaact
    25441 ttgtaacaat atagtttttt taatgtgcgc agaaacaatg gtgtcaatgc ttccggcaaa
    25501 tgcaaagcgt atggcattta gattatttac aataggatgt tttgatgttg gagtcaccaa
    25561 aggataataa aaccagggca acaaagaatt ctgtggtctg ttacctacat atcctgtcag
    25621 tataggaatc ggtaccgact gtaaatccat taccaagtct ttattcagtc ttacaccata
    25681 acgaaacaac atgtcatcaa gattcagttc tttaggaata gcaatgaatt cgttttttct
    25741 tgctaaacta tccatatcag cactcatcat atcgagcaac caaagaacct tgccgccacg
    25801 catgataaac tgatctatta taaacttatc tttttcatca aatgcagagt caggctttgc
    25861 gattaccaaa caatcataat cttgtaaagc attgagctga ttttttattt gcacataatg
    25921 cgtagggtag gaggcagata cagatttata aaaatcagcc agataaggtt tgtccaattc
    25981 tccctgacca tgtaaaatac caattgtcgc tggctttctt gtcgaaattt tagaaatggc
    26041 attaataatt tcatattcca gattctgaat agaagtgttc accatctctt ctgcagatgc
    26101 acccattcta tctttcagca gtagtagtgg ttgctcttta gaaaggtagt taagtattgc
    26161 tcccggaaac aaaattcttt ctgatgtgcc ttctgcattt cgctcagaga tattggttgg
    26221 ttgaagccct ttgtctgcaa gttgattaaa aagctcatta cgttgcttgc tgtctgctaa
    26281 tgccgatggg tcaataaaat caaactcaag atttgagcct gcatagctct tcatttccat
    26341 cagcatgtcg cgtgttgcag aacttagcct ctgaaaacct gcaggtaaat cacctgtcag
    26401 ataaactttt acatatacta tatctttaag ttgctttagt ttttcttttg ttgtgggcga
    26461 aagggtaaaa cgtttttcag tagtaaggtc aaaacgccag aataaaaatt taccggcagc
    26521 attgaggcct gcaatcacta aaagcaaaag caaaaactga tagaggttgg cactgcgtat
    26581 cttcttcttc atatcaccac ttcctgcttt cgagttttaa tcgggtaaac actaaaaata
    26641 acgctgtcaa actgataaaa taaattacat cgcgcgagtc aataacacct ctgctcattg
    26701 agttgtaatg tgccacaata ccaagttgat ctatcaggtt tgcaacaaca cctgttgcca
    26761 gaattgttgc aacagactga aatcctgtgt agcatagcag gcataaaaac atggctatga
    26821 taaatgctac caattgatta tctgctaatg atgaagcaaa aacaccaatg gcaacaaatc
    26881 ccgatgccag aaaaaacaat cctatataac ttcctgcaat tccacccaaa tcaatatttc
    26941 ctacgggtgc agcatattta taaactgtaa ccacataaac caatgttggc agcaatgaaa
    27001 atattaccaa taccaatcct gataaatatt ttgccaatac aatctgaaac tctgttaaag
    27061 gtttggtaag caatatctcc aaagtccctg tacgtttttc ttccgaaaac atgcgcatgg
    27121 ttatggcagg aatcaaaaac atgtaaaccc atggtgtaat gttgaataaa ctatctacgg
    27181 cagcataacc actgtctgca atgttgaggt cgttgcccgg aaacacccac agaaataatc
    27241 caatcacaat taaaaaaaca gcaatggaaa tatatcctat tagtgaatta agaaaactgt
    27301 ttatctcttt cttgaaaagt gaatacataa taatttaaag cagcaatgct gaaataaaga
    27361 ctgccaaaaa tacagtttcg ttgcatatag gcagttgcag attatgaaag attaaggaag
    27421 cttataaaaa caaaattgtt aattaaataa tttgcttgca gaagtacttt tcgatgaagc
    27481 caaatttctt aatcttgctt tttcaaaatc atgggtattt acattaaatc ttcagtatgc
    27541 atcagcgcca gaaatacttt tggcgaaaca gatctttcag aacatagctt gcaaacagaa
    27601 aagcttatgg cgcgcgaacc tgtttacagt atgattgcac ctgccgtttt gcgaagaatg
    27661 ggtaaagctg tgcgcatgac ctgcggtgct gtgagcaaac tcaatctgga aaatcaccct
    27721 gtaaacggaa taataatagg tactgcaaat ggcggtatgg aagattgtat tcgttttctt
    27781 aatcagattg tgcagtatga tgaaggcaca ttaacaccaa ccaattttgt gcagagcaca
    27841 cccaatgcca ttgcttcaac cattggaatg aatcatcaat gtcgtggcta caacataacg
    27901 catgttcacc gtggcaatgc ttttgaaaat gctttgcttg atgctttcat gtatttagta
    27961 gaaaataaaa actcaacctt attgctcggt ggcattgatg aaatttctga ctataattac
    28021 aatatagaac gcttgggtgg atggtataag aacgaaattc ctgtaaatgg tttgtatgaa
    28081 tcaaaaacag aaggaagcat tgccggtgag ggttgtgcag cgtttacagt aagtaacaat
    28141 tcagaaagtg cactgttaga aattgttgac gtaagcacac ttcacagtac tgatgttgac
    28201 gaagttttac aacagctaaa acaattttgc gataaacatg ctttaaatct ttcggaaact
    28261 atgttggttt caggtcacaa tggcgatgtc aggaatgaaa cattgcatta tgctgttgaa
    28321 agtcattttc agcaatctgc aatcatcaga tataaacatt tttttggtga atttccaacg
    28381 gtgtcggcat tggcactttg gcttattggt caggctgcaa caaactccgg tattttgcct
    28441 gaacattttt acttacgtaa gtcagaaagt aaaattaaaa actaccttat ctgcaatcat
    28501 tacaaaggct atcagcatgg ttttatattg actaaaacac cataagtaaa tatcagccta
    28561 tttctgcaag ttccatccaa cgcattgtct ttgcatcaat gtcatgcagc acctgctgaa
    28621 agcgattact tacttccatt aacttttcat gtgtaagttc tgcttcgccc atctgttgtt
    28681 ccaacattgt tttttcctct tcaagcagca taatgtcatt ctccaacttt tcgtactcct
    28741 gcttttcttt gaatgaaagc ttgcgttttt tattcgctgt tgtttcttcc ggttttgttc
    28801 tcactactgc tgctgcttcc tttttctgct ctcttttttg ctccttcagc atttgtctgt
    28861 agtcatagta attgccattc agaacactga tttttccatc tccttcaaat gcccagatgt
    28921 ggtctgcaac cctgtccata aaaaaacgat cgtgcgttac aataatcaca cagccttcaa
    28981 acgatgccaa ataatcttca agaacagaaa gtgtctgtat atctaaatca ttggttggct
    29041 catccaatat caaaaggttc gggtttttca tcagcaccgt gagcaaatac aatctgcgtt
    29101 tttcgccacc actcaatttt tctacatact gaaaatgcat ggacgggtca aatagaaaac
    29161 gttcaagcaa ctgcgatgca gatatttttc gtccaccact catcggaata aagtcagcaa
    29221 tttcacgaat cacttcaata acccgcttct gatctgaaat atttttcgat tgctgctcaa
    29281 aatatccggg cacaatagtt tcacccatca caatcttacc tttatcgcaa ggctcttttc
    29341 ccattatgat attcagcagt gtactttttc ctgcaccatt gctgccaaca attccaattt
    29401 tctcgccttt acgcagcgag tagtcaaagt tgtcaatcag tttcaggtcg ccatacgatt
    29461 tgctgatgtg gtgtatttcc agaattttac ttcctattct cgaaatgttg atgtcaagtg
    29521 ccacattacc ttgttcattt ttctttttag ttttttcttc aagcacatga aaggcatcta
    29581 tccgcgattt tgattttgtg ccacgtgccc gtggttgttt tcgcatccaa tccaattctg
    29641 tacgaaatag attttttgaa cgctgcaggt ttgccgattc catttcatca cgctctgctt
    29701 tcttctctaa atagtagctg tagttgccct tatgtctgta cagtttcccg tcttcaagtt
    29761 caagtatttc atcacatatt tcatctaaaa aataacggtc gtgtgtaaca agcaacatcg
    29821 ttatgtttgt tgatgctaaa aacgattcaa gccattcaat catgtctaag tctaagtggt
    29881 tggtaggctc atccataatg ataaagtcag cctcatcaat cagcactttc gatagcgcca
    29941 aacgtttttg ctgcccgccc gaaagttcat tcaccttttg atgaaaaaat ggaacaccca
    30001 attttcctgc aacagatttt atttttccca aatgtgtttg ctcttcttca gtcagttcat
    30061 aatgtggttt ttcattcagc aaggcatcag aaatgtgtgt gttctctgtc aggcaatctg
    30121 caaccgttgc caactgtggt aaagtatttt cctgagtaag ataactcacc ttcaactgat
    30181 ttctgaaagt aacagtacct tcatcaggaa catcaagtcc tgctaaaatt ctcagtagtg
    30241 atgttttacc ggtgccattt cttgccacca atgccagttt ctgtcctttg gcaatgccga
    30301 acgaaatgtt gttgaataat actctgtcgc caaaactctt gcttatattt tctaccgata
    30361 aataattcac tgctttttaa ttgaagcgcg aaagtactac ttatcatgct atttaatggc
    30421 aatcagcatt gtttttatat acggcaattt ttctcttgct tgaatttaat ttcatagaaa
    30481 gttttaattg acaaaaatca tttttacaat cataaaatat aaccactatt gcgttaataa
    30541 ttttatttct ctcaacaaag cacatatata gttttttgtc atttttgaaa gatgccattt
    30601 aagaaaagca tatacataac aacatcagca attctcatca ttgtagcatt actgcaatgg
    30661 tcgttcataa cggtcaaaga aaatgaatat cagcagcagg cattagaagc ctatctctca
    30721 aaatcgtcat tagatttctc tggagaactc gaaaaactta aaattcttgc cggcagcaat
    30781 aaagtaaact cacagcagtt gattgcacaa tttaaagcct gtcgccttgc ctataaaaaa
    30841 ctcgaaggct tcaccggata ttattttgct tatacagaac gcgacctcaa cggcccgccc
    30901 attccaatgg tcgaagaaga accgcagagc aaatatgttc gtccaccaca tggcttgcag
    30961 gtaatagaag aaataatttc cgaaaaacca ttgaataaag cacaactgct cgatgaaatc
    31021 aatcacacac ttggtctggc tgccaagtta gccacatcgt ttaaaacact gcacccctac
    31081 gattatcagt tgttcgaaat aatgcaactg cgccttgtaa aaatttacac attgggtata
    31141 aacaatttcg actgtcagat tattaagaca ggaataccag aaactaaagc agcactgttt
    31201 tctatcagca atattttaaa tatctcttat cgcaatgccg atttttcaat ggtcaacaac
    31261 agcattgact ctgccgtagc ttttctcgac cgtcagcaaa taagagtaaa ccaaggtgcg
    31321 gagttcgatt actttacatt ctatcgcaac tatgttgtgc ctgtaaattt atcactcatc
    31381 aatgcaagaa aaaatatttt aaaattcgac ccgcaaaata taagagcctt aaatcttgat
    31441 gctacaacac tttttgagaa agaagcctac aacacctatt tctttaccaa acttcagcgc
    31501 agcaacaacg gagatatgct tccttatctt ggcaaacttt tatttttcga ccctatactt
    31561 tctgtcaaca atgctgtgtc atgtgcatcg tgccataaac ccaatatggc ttataccgat
    31621 ggccttccta agtcttatgg ttttttaaaa aacgacacac tgcctcgcaa
//
LOCUS       DAOOOL010000011        29630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_120_length_29630_cov_126.089366, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000011 DAOOOL010000000
VERSION     DAOOOL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 29630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29630
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120_length_29630_cov_126.089366"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKU82_01935"
     CDS             <1..861
                     /locus_tag="PKU82_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOZ81662.1"
                     /translation="TYAEIPYDVVYDEEIVQGKLALYDWMHLHHEDFTGQYGKFYNMY
                     RNATWYQEQQRSMESMAKQLGFAKVSQMKLAVAKKIKEFVAGGGFLFTMCSGTDTYDI
                     ALSAEGLDICESMYDGDGTTPNYNDHLDFSKTFAFRNYTLSVNPLEYRFSNIDVTTTR
                     RVPRDEDVFTLFDFSAKWDPVPTMLCQDHMQVIKGFMGQTTAFNKNYIKPDVIIMGEN
                     KAANEAKYIHGDFGKGTWTFYGGHDPEDYQHMVGDPPTDLNLHPNSPGYRLILNNVLF
                     PAAKKKKQKT"
     gene            complement(858..1691)
                     /locus_tag="PKU82_01940"
     CDS             complement(858..1691)
                     /locus_tag="PKU82_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ81663.1"
                     /translation="MITRKQPVTMMYSKALIKPDGEVLNYSDTSSGNEVLVLIHGYPF
                     DQSIWKEQVIALSDQARIITYDVRGYGSSSKGKERFSIDLFADDLIFLLDSLQVSKFI
                     VCGLSMGGYIALNLVKRYPERIKGLVLYDTQCYADNAIGKAKRYENIELIRKGGKQKY
                     LEDMCKILFSPATFQKNDVVIKQTLDLMMKADNEVLMQTLEAMAEREESCSNLSSIKF
                     PTMILCGEYDVLTPPEKSEFMQQQIPGSVLHIIENSGHLGHLENKDAFNQHLNSFLKS
                     F"
     gene            complement(1706..3478)
                     /locus_tag="PKU82_01945"
     CDS             complement(1706..3478)
                     /locus_tag="PKU82_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOZ81664.1"
                     /translation="MEVTRLIDLFPYQLSRFPKADALAGKQDGVWKKYATADIIETIN
                     NLSYGLLHSGIRPGDKIGIIANNRPEWNFVDHACLQIAVADVPLYPTISENDLKFTLN
                     QSAVRYLFVANVDLYKKVSQIKNDLPALEKIYLFDRYQDLPHWSELIDLGLKHPKQEE
                     VKQIRDSIKPDDLATIIYTSGTTGNPKGVMLSHNNLVSNFKFCDVLCPFHESWNALSF
                     LPLNHVYERMLSYLYVYFGVSIYYAESLDTIADNLKEIKPQIFVTVPRLLEKVYDKIV
                     SKGAELKGIKKALFFWALNLGLRYELKGANGWWYEFQLRLANKLIFSKWREALGGNIQ
                     AIASGGAALQPRLARVFHAAKIPVLEGYGLTETSPVIAVNNFQPDSIKFGTVGTVLEN
                     VEVKIAADGEILCKGPNVMLGYYNSPELTAEVIDADGWFHTGDIGVFEDGKFLKITDR
                     KKEIFKTSGGKYIVPQMIENKLKESRFIEQAMVIGENQKFASAFIVPNFAFIRDWAAK
                     KNIAVNSNAEIAASNEVKKRIAEEVEVVNKTLGHYETIKRFELLDREFSIERNEMTPK
                     LSLRRKIILENYKDKLAKIYKGTE"
     gene            3547..3930
                     /locus_tag="PKU82_01950"
     CDS             3547..3930
                     /locus_tag="PKU82_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HOZ81665.1"
                     /translation="MNYNDLLNKSKPLLIFDGSCAFCRSCIRFVKKYDIKLQFDFADN
                     NQNTTKLILAQYFINPESLNSVVLIEYNKLHIQSDAIIKTLLLLGGRWRLLARIMMVF
                     PNSFRNKVYNIIAANRNKLRSHCTV"
     gene            3927..4925
                     /locus_tag="PKU82_01955"
     CDS             3927..4925
                     /locus_tag="PKU82_01955"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOZ81666.1"
                     /translation="MKTNFTEQTGIAYPIIMAPMFLVSNEAMMQSAIRTGIMGVFPTL
                     NYRKEGELDALIKRLHQFKSENQGSFGVNLIVQKTNPLYNRHLKICVENKVPFYITSL
                     GSPREVIEAAHSYSAKVYCDVTNIAHAEKCAKLGCDGFIAVGQGAGGHAGPYAQTVLI
                     PTLRKHFPSIPVIAAGGIATGDGIAAMIALGATGVSIGTRYIASTEAEVSDEYKNSIL
                     NSGMDNIVMTERLSGTPCNIINTPYAKKIGYKQNWLEKWLSNNSTTKKYFKMMVQYKG
                     VKALEQAIKPGNYKTLWTAGQTVELIDDIINCDEITERLIKETHLSVENLSNKFKD"
     gene            5078..6571
                     /locus_tag="PKU82_01960"
     CDS             5078..6571
                     /locus_tag="PKU82_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81667.1"
                     /translation="MAFKRIFFFVWFSVCFHVAHAQKVTYAQQELDAYSYDYIKTAGY
                     NDNSFFVFQSNLPFENDRDRVGFKSRKYKVTCYNLNLSLKWSKKIEPKNDNEKLETIT
                     VIDGQVALIKSSFEGKDNQLSVFITTLDDNGNESATKKEIGTILFDRTSNLDKIKVIA
                     SKGNNYFGLIQNEKKQNGLQQLHCLIIDSDFKLVRQLQFEVPYPEKNFNYENWLLTDD
                     ADFILEGSYYSKEKSENRKRWDAYKLFVSSNGNSGAIEYAINTSNLILSSSTIAYDAI
                     NKKIVLAAFYTDNVSTKSGIIYASLGTSGNDSLLMIRQPIGEVTLTRMFAGQNLDRSR
                     AGIENLSIEKVILRNDGGAVLVTEVSYVTDYSYYDYFTQNYIRNFEYHFNNVITFSIN
                     ADGTIDWDAVLRKDQISTNDYGKYSSFALAMTENDISLLYNTRTDRNNGVAYFGIKRN
                     GETESKEIIKPEERILLVPFAAKQISTDEIVVPCWNRKKMLMAKITF"
     gene            6526..7350
                     /locus_tag="PKU82_01965"
     CDS             6526..7350
                     /locus_tag="PKU82_01965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ81668.1"
                     /translation="MLEQKKDANGKNYFLMPGSLSIALQYISYYVKAWNKHSVHSPFV
                     YKLTTQVIHPDKKLAKFIPIEQQRILLKQSTDKIFFTELGAGNKSNKRSVSEIAAKSL
                     KFPRYARLLYRLANHFQPDNIIELGTSLGITTGYMALSGCKNIFTIDGNKTVSEIAQS
                     TLTSIKLHQNIKFLQGNFDEALPALLHPLNKIDFVFFDGNHRYQPTINYFHQCLTKAH
                     DKSVFVFDDINYSQEMKQAWHEIKQHPSVIVSIDLFMMGIVFFDTDLLRQHFVIRY"
     gene            7409..8128
                     /locus_tag="PKU82_01970"
     CDS             7409..8128
                     /locus_tag="PKU82_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ81669.1"
                     /translation="MPQSIISLSDISRLYKVGTETIYALRSVSLNIFKNEYVALMGSS
                     GSGKSTLMNILGCLDTPSSGSYHLNNISVSGMTDNELAEVRNKQIGFIFQTFNLLPRS
                     TALENVALPLVYAGVSKEERLKRAKEVLESVGLADRIMHKPNELSGGQRQRVAVARAL
                     VNHPSIILADEPTGNLDSKTSEEIMLLFEEIHQRGNTIIVVTHEEDIARHAHRIVRIK
                     DGLIEDDAINTQITKAKTIVS"
     gene            8125..8835
                     /locus_tag="PKU82_01975"
     CDS             8125..8835
                     /locus_tag="PKU82_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ81670.1"
                     /translation="MNIKSIQSLLIVVVLLASCGVANSQKGKLLTPEVFEKMLHETPD
                     AQVIDVRTPEEFNDGHLKNALNMNVNSKDFENRAPYLDKEKPLFVYCYSGGRSAKACE
                     YFEKMGFKVIYDMEGGYSSWTDANLPVEGKKADKAGISLDNFALLTSAGKVLVDINAP
                     WCPPCVKMKPMLDSLDNAWKDKVKIVRLNKDENQLISKSLQINELPTLLFYENGKLVK
                     RESGYKNAEQLKAMTQVE"
     gene            9093..9593
                     /locus_tag="PKU82_01980"
     CDS             9093..9593
                     /locus_tag="PKU82_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81671.1"
                     /translation="MNISALAEAEKLLFIARQSLSEGKLPQAIDMLNKIVTDYPDFSK
                     AHITLADIYFNKLQDSTTAEEYYKKAIAIADMNTDAFTGYANLMLQQEKYPEALSMLN
                     KAMAIKGAPKDTIYMLLGKLYEMQGRLDDAIGNYKKCITTTLSNSVLEEAELALKRCE
                     VKKKYI"
     gene            9746..12946
                     /locus_tag="PKU82_01985"
     CDS             9746..12946
                     /locus_tag="PKU82_01985"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ81672.1"
                     /translation="MKRVFTISLCFFVFYSISFAQLDQYASEQNKFYWKNRKPTAGYW
                     QQDVYYNIKAAIDEKTDIISGVEELTYRNNSPDTLKFIFMHLYQNAFVPGGYLNDLNK
                     ANQNKVRYGKYELQGLGIEITNINTLSVNGIIINKQTEKIIDNTIMKIMLPQPLMPGH
                     DLVLKIDFKTYYDKTGQRRRLQMFENAGNKHYNGCQWYPKMCVYDSHRAWNTDQHLGR
                     EFYGEYGVYDVELTFANNFIVEATGVLVNEKEVLPDELRKKLDIKNFKERPASPQPST
                     IIIPDGSTKTWKFHAENVHDFAFTADPSYRLSDAYWNGVRCVAIAREQHCYGWQSAAE
                     YTARIIEVFSRDFGMYDYPKIVTADANDGMEYPMITMDNGTDPGFRGLLCHEIGHNWF
                     YGMVGSNETYRPLLDEGFAQLLKSWGMEILEGYHIKQRETTWKDQKRTEPLTYQMTSA
                     YGLYYNYVLNDKDGFINTHSDGFSSALGHGGGYGMVYYKTATMLYNLQYILGDSLFLK
                     AMQHYVAQWKFCHPYPEDFRNSITEYVKTNLSWFFDQWMETNKKLDYKICKYRKADEK
                     DAFNVYFKRKGRMQSSIDFSVYSNCGVKYDYHIPNNWFVKQTDATVLPKWEGWDNLRN
                     TYKAQIKIPCGISSIVIDTTDRLGDAYRLDNRLPRPFKLVFDKWQKPAYSDWKSYVFY
                     LRPDVWFNNYDGLKVGVHAESNYMNTMHFFSGDVWINTTVGQNSLSETADINKNDPAS
                     FRINYKTPLKFVAPGVNFWIGGGLLDGLTFAKSGGDLTFLNNRVRLFAEYKLMYRRNT
                     NTLEYLLYPNEWQSALMNTSVTLGAEAQYRKNKWNGSVVIQNTSASLFSDYSYSKLSL
                     TNITNYETGKIDWRLRVFGQYGYGQNVPMESALYLAGANPEELMESAWTRSRAFVPYS
                     WLGYGDKTNHFQQGGGLNLRGYAGYLVPQNIDGEQRYFYRAISGASVSLEIDFDRLIP
                     LRPSFTKKWLKADLYFFGDAGAIATNNSNKSIQFTNLKSDAGIGTAFTIKKFGRLNAI
                     KPLTIRCDFPFLLSSAPYAEKDYVKLRYVIGIGRTF"
     gene            complement(12957..13448)
                     /locus_tag="PKU82_01990"
     CDS             complement(12957..13448)
                     /locus_tag="PKU82_01990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOZ81673.1"
                     /translation="MLTELYTSYILTPLGYMNAITDTDSVLALEFDDKYTSGEILNGA
                     VSIQIANELQEYFSGKRKLFTLNLKPQGTVFQLQVWEKLLSIPFGKTISYNEQAVQLG
                     SAKKIRASASANGKNPIAIIVPCHRVIGTSGALTGYAGGLWRKEKLLQLESGSQQIEL
                     FSQ"
     gene            complement(13485..14639)
                     /locus_tag="PKU82_01995"
     CDS             complement(13485..14639)
                     /locus_tag="PKU82_01995"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOZ81674.1"
                     /translation="MKNKFWLAIAALAATTIVSCDKVDGPYKEENTTNIGTLPGTLND
                     IRVLNATTTADDVDVLVAKFSLESSSPKTIAVQFTWHGAQSNVISEVATGNDAIFDND
                     SVYITAPAPFGTLTFKYSATISDTLTGQADFVVPYTPANPVKKVLLEDYTGRKCGNCP
                     RAQRIIANTLEPLYHEQLVVATNHAGVYAVPDNPPSCFSEDFRNSNSDELNTFFGLSA
                     YPSSMINRVGYPTTHIKYYNSWQSVISQELSKPNDAFINIYSAYSESNRTAQLTVSTE
                     FLNNLGDDYKLAVFLVEDSIHGCQLDYDLTTPTQIDSAYIHRHVLRAALNSSFGESLI
                     NDPDLTTKYIRLYNYQIPDKYNVDQCYILAYVYQTNNYYVLQSEIRKVKP"
     gene            complement(14660..16294)
                     /locus_tag="PKU82_02000"
     CDS             complement(14660..16294)
                     /locus_tag="PKU82_02000"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOZ81675.1"
                     /translation="MIKLITGRILPVIFLGAPTLSLAQSSPGEIHGSFQIDGQYYIQD
                     STISAPEVAEKYLLNGYGDLVFTKGNFKAGVRYETYQNVLLGYDKRFNGSGIAHRYAE
                     YDNGELAITVGNFYEQFGSGLILRSYWEPTLGYDNALDGVRVKFKPYSGILIKGIIGK
                     QRYYFKTGEGIVRGVDAEVGINDLIKKWNDKKTRVTAGFGFVSKYQKDDDNVYILPEN
                     VGAYAGRLDVTRGKVTINAEYAYKYNDPSSLNNYSYKFGDAALLKATYSQKGLGILLA
                     AKRIDNMNFRSERNANLNDLLINYLPALTKYHTYSLASIYPYATQPNGEFGFEAEVNY
                     TIKKGTKLGGDYGTNIIVNYSGANSLSTEPVAGKDLYKSDWGKFGKDVYYKDFSLEIT
                     RKMSKNLKITLMYINQVYDKDKIQFQGSKQYGTIYDNIIVADITFQLNDKNAIRTEWQ
                     TLQSEQDYKSWIAGLIEYSHSPNWFVAVSDQYNYGNEKSDKRIHYFNCFVGYTKDSHR
                     ITLGYGRQREGLFCVGGVCRQVPSSNGLTVSISTSF"
     gene            complement(16324..16836)
                     /locus_tag="PKU82_02005"
     CDS             complement(16324..16836)
                     /locus_tag="PKU82_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOZ81676.1"
                     /translation="MNHLKKLVVVFSCAVSVSSAQVFESRPIPAVDVKDLNGKTVNTS
                     TLNNNGKPMVLDFWATWCKPCVNELTAISEHYEEWQKETGVKIVAISVDDARTMANVK
                     PFVNGKGWDYEVYVDPNGDLKRALNIGAVPYVFLVNGKGEIVSVKTGYAPGDEHKMYE
                     EIKALTKESN"
     gene            complement(16857..17558)
                     /locus_tag="PKU82_02010"
     CDS             complement(16857..17558)
                     /locus_tag="PKU82_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81677.1"
                     /translation="MKKIVLALILITSASLVKAQSFSYTGLSADVYGSVEDSLLHSYV
                     YVINNSQNTIDASLNAYDVYKVPGTEHNFCWGILCYGVGVMSSAIPATINAGATEGSF
                     RGDYLGHGYVGTTTVAYTVFDGNNPLDSVQFAINYHITPVGVKEVNKTNSISAPSPNP
                     ARSITAFNYNIKSGSKAEVKVINMLGKTVKNFNVNSSTGNIVLSTADLPSGVYFISLN
                     VEGKSSAVQKLVVNR"
     gene            complement(17616..19499)
                     /locus_tag="PKU82_02015"
     CDS             complement(17616..19499)
                     /locus_tag="PKU82_02015"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOZ81678.1"
                     /translation="MKKIFTILTAACLVVGIANAQSQRYVVVEEFTGETCGPCAANNP
                     GFNTKLNANAFTIPIKYQNNIPSNGPNYYSYNTIDVANRTSFYANNYSPHAFLDGNVW
                     DGNAGSFPVATLNSRVATTSPFTVDVTHSFSPTHDIIYTHTVIRATQAVTGLTSLKAR
                     IAITEKDLYGYTSPNGESHYSDVMRKMLPNGTGTILPATWAVGDSVVLDEQWTINVPT
                     NIPTVFMPYWPMLRAVAFVQNDATKEIMQGGRSSAAAVVPLAAITNLASIFTCTSSID
                     PSVTLKNYGGGGDLTSADIEYGIVGQTMQTYNWSGNLGSNTSVVLTLPNLTLPGAGLQ
                     NFSAKLISVNGNAAFHNINLDYKTSVAQTTAPVTSLSQDFLATTFPPVNWILNNPDNA
                     TTWVRSAAGSLAGGSAKMNFYTYAAAGQSDYLYPYTPIDMTNAVNPQLTFSVAYCQYS
                     TENDRLQVLASTDCGVTWTSLFNEAGAALSTSPAQTTAFTPTSASQWESKTVSLANYV
                     GQPSLLLSFKATNAYGNNLYVDDINLTGVTAVHQIEGVTTLEVFPNPASDVLNIKATM
                     EKSLPMTISLTDISGRKLFQKNLGTVSVVNETVKTSDFAAGIYTLEVRSGDSVSYQKI
                     TIK"
     gene            complement(19630..20601)
                     /locus_tag="PKU82_02020"
     CDS             complement(19630..20601)
                     /locus_tag="PKU82_02020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ81679.1"
                     /translation="MSEVQLRQLLKDEIGLLPGLMENCFSMKVDKAYFKWKYLDNPAG
                     NFIGFGAFFENKLVGFYGVIPERYQINNSEVIIFQSGDTMTHSTQRRKGLFEKLATLC
                     YNYVREHHQLAVIGFGGDKSTPGLVKMGWCHIADVKYFFIHALHCKWINNFTTEDRNF
                     NIVEKTDIHVILDIQKNFGFKKNIKQHYSKEFLKWRLANPRFNYRCFICYQGQNPVGY
                     VICYEDEGKLFLLDYCSISVKVQKTLIRRLSQICIKSKLKGIITIAIPKSEEGKWLKK
                     AGFLRNPFKKGPLTTNIPFMILTDQKPESQLCSKDQWQVTPICHDAL"
     gene            complement(20598..21542)
                     /locus_tag="PKU82_02025"
     CDS             complement(20598..21542)
                     /locus_tag="PKU82_02025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ81680.1"
                     /translation="MLKLIPASPNGVTILCFHRISSQYDYFWQPIYPETFRLMLESLV
                     KEYQIIPISQIENKSFRSTKPPLVLSFDDGYKDFIEEAIPVLHYYEAPSNHNIVIDCA
                     DGKSYIWTQKLNHLFNLLRNSSRFSINIALDKNFVLDIKQPCKNWFAHYYKTLFYLFK
                     LDVIDRSECIAVLEQYLNITETDYPIKMMNWDDIKFIATNNVEIGSHTASHVSLSAVK
                     NDVTFEHELNYSKKRIEEMLKCECPVIALPNGLSNGEANQKISEAGYKTVLCLAGSGS
                     NAGTSTDKIRFYNRLNMIQEPNSYMNVRIKLYEKGFIK"
     gene            complement(21633..22157)
                     /locus_tag="PKU82_02030"
     CDS             complement(21633..22157)
                     /locus_tag="PKU82_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81681.1"
                     /translation="MNFDNLTTALLIIAPTFIVAGGMYMLIKNMLERDYRLKLLDARR
                     LIQKDMLPLKLQAVERMVVFLERIQPENILFRILQPGMNVRDLQVDLLAAIRQEYEHN
                     VSQQVYVSVQSWSLITKAKDDIIATVNLAADSLDKTEKAIVLSQIILQRSSAGNNFAG
                     AAILSLKSEVDSLL"
     gene            complement(22160..23803)
                     /locus_tag="PKU82_02035"
     CDS             complement(22160..23803)
                     /locus_tag="PKU82_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81682.1"
                     /translation="MKASRLVFILILSFAFQLNISAQYVPQSVFNKPLYDFMDDLANL
                     HIITLNDAIKPYSRKQIAEALQTAESQKEKLNATQQKQLAFYLKDFNKELFTGKGFKK
                     RLDLFYFKDSLFNITVNPVGGFSYYKNENDNMYRQYAGGEFWATLGKGLSIFGSYRDY
                     TDSRAVADDKYLNDLPGYNYKYKGAVGSPKRGGSFDETRGGIAYAWKWGHVGIVKDNF
                     EWGSNNHGANIISTKAPSFARLELKLNPKKWFEFNYFHGWLYSEIKDDSAAYFSGNPP
                     NHRQVYFSKYFAANMFTVKPFKQFDFSFGNSIVYSDQLEVAMFIPFMFFKSLDHSKTG
                     QGSNYSGQNSQLFFNISSHNIKYTHLYISLFVDEIAFGRMMDKNEHSNFYSLKAGAAV
                     MLPFYTSATFIAEYTRTNPVTYRHFVNTTTYESNRFNMGHYLRDNAQEIYLGARCAPI
                     SGLYISAGYTSAAVGPIYPYTGKDKSGLGLPFLKTKEYEFTSLDLNIKYELFNDIFLS
                     AGVKIDDNKGAMANVYTAPFYYGKNGKTTTLFGGFNIGF"
     gene            complement(23863..24975)
                     /locus_tag="PKU82_02040"
     CDS             complement(23863..24975)
                     /locus_tag="PKU82_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ81683.1"
                     /translation="MIFKTDNTEAKLLQLEEFAELDAVLATKAKNDVRNERFVFNLIE
                     KYVDTSRSDCFTFAANNHDDANQLADETYHAVIHFKRVNNFQFINKQFEAINTKLPKG
                     GIFVSKVETNDLRKQRLMEKYPPYLNKVYYFFDFVFKRVFPKLPVTQQIYYAITKGHN
                     RVLSKTEMLGRLYSCGFKVIEERYIGNRLYIVSEKFRDPAYDEDPSYGVIYPMKRVGK
                     GGKIISVYKFRTMHAYAEYLQEYVYEKNNLEEGGKFKDDFRVSRVGRMLRKYWIDELP
                     MILNVLKGDLKIVGVRPLSRHYLSLYSKELQDMRKKFKPGLIPPYYVDLPKTMEEIMD
                     SEMRYLKAYQKSPFLTDVRYFLMVFYTILFKRARSK"
     gene            complement(25115..26128)
                     /locus_tag="PKU82_02045"
     CDS             complement(25115..26128)
                     /locus_tag="PKU82_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HOZ81684.1"
                     /translation="MGHPAHFHLFKNTIIALQQSGHEAMILIKKKDILEDLLRHAGMQ
                     YININPEGRRDDKFSIALKLLKRDFQFLKICLRFKPQIMIGTSAEIAHVGKLLGIRSI
                     VVNEDDAEVVPWFAKLAYPWATNILAPYCCSVGKWEHKKIGYKGYHELAYLHPSNFSP
                     KKENIKNLGDEPFFILRFAKLTAHHDEGRKGINTLIAEKLINMLSKHGRVYITSEREL
                     ESQFEQYRIAINPLHIHDALYFAQMYIGDSQTMAAEAAVLGTPAIRFNDFVGQISYLE
                     ELEKEFALTFGFKTSDADAMLVHVETLLNQQNLKLQWQEKRSMMLNQCIDLNQFMIKL
                     LTQ"
     gene            complement(26155..27030)
                     /gene="dapA"
                     /locus_tag="PKU82_02050"
     CDS             complement(26155..27030)
                     /gene="dapA"
                     /locus_tag="PKU82_02050"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOZ81685.1"
                     /translation="MNKFTGTGVALVTPFLKNGKIDFNALRKVVNHVIKGGVEYLVVM
                     GTTGESVTLSKEEKKDILKFISEVNNKRTHIVFGVGGNNTLETARELEQIDARMADAI
                     LSVSPYYNKPNQSGIVQHYKTLSGVSQLPIILYNVPGRTGSNLLPQTTLEIAASCKNI
                     IGIKEASGNMEQIMYLIQHRNKGFLVISGDDAITMPLIAAGADGVISVIANAFPKQFS
                     TMVRLALQGKITESRKLHYQLLDLIPLLFAEGSPSGIKYVLSQLDICQNYFRLPVASI
                     SKSLAQKINTVIKGI"
     gene            complement(27027..27581)
                     /locus_tag="PKU82_02055"
     CDS             complement(27027..27581)
                     /locus_tag="PKU82_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81686.1"
                     /translation="MKIGKAIKQKAASYLFYRELQRQHHQPRTTSFSNARTIGILYDA
                     TIERNYEIVKAYVKHLRDDFKKDVLALGYYDGNELPPMRFSKLGLDFFTKKNLNWHFK
                     PSHPMISKFVNLDFDYLICLNVDRCMPLRYISAATKAGFKIGRYEKGYAGLYDFMISV
                     NGQVTLPQFIDHVNNYLKHIGHDK"
     gene            complement(27578..29590)
                     /gene="ligA"
                     /locus_tag="PKU82_02060"
     CDS             complement(27578..29590)
                     /gene="ligA"
                     /locus_tag="PKU82_02060"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOZ81687.1"
                     /translation="MSKISEKEAGEKIKQLTVDLNHHNYLYYVKAQPVISDYEFDVML
                     QELIALEKQFPNLLTTESPSQRVGGFISKEFVTVTHSYPMMSLGNTYSEEELRDFDER
                     VRKVIGSDFEYVCELKFDGVAIGLTYQNGKLLRAVTRGDGVRGDDVTTNVRTIRSIPL
                     SLMGNNFPDKFEIRGEIILDKKTFERINAERADIGETPFANPRNSAAGTLKLQDSSEV
                     ARRKLDCFLYALYGDFDFSTHYDGLKMAADWGFKISEHYQLCKSIEDVLTFIESWRKK
                     RDLLPFDIDGIVIKVNNFRQQEELGFTAKSPKWAIAYKYKAESASTILNSVVYQVGRT
                     GTITPVANLKPVLLAGTTVKRASLHNADQIQKLDLHMHDTVFVEKGGEIIPKITGVDF
                     SMRQAGAKPVSYITHCPECGTELIRLEGEANHYCPNEEGCPPQIKGRLVHFTSRKAMN
                     IDGLGVETIDQLVEAELLKNVADIYHLKSEKLLQLERMGEKTVSNLFEAIEKSKQVPF
                     ERVLFAIGIRFVGDTTAKKLARHFGSYQKISQATLEELIQTPEIGDKIATSIFDFVRE
                     LENIKMVEQLQSAGLQFKLDKAAMQEAGSKLKGLSFVITGVFQKFERDKLKETIEING
                     GKVSGSLSSKTSYLLAGSDCGPAKLEKAEKLKINIISEDDFLKMLQ"
BASE COUNT         9481 a         5540 c         5065 g         9544 t
ORIGIN      
        1 acctatgctg aaattcctta tgatgttgtt tatgatgaag agattgttca gggcaaatta
       61 gcattgtatg actggatgca cttacatcac gaagatttta ccggacagta tggaaaattc
      121 tataatatgt atagaaatgc aacatggtat caggagcagc aaagaagtat ggaaagcatg
      181 gccaaacaac ttggatttgc taaagtgtca caaatgaaac ttgccgttgc aaagaaaata
      241 aaagagtttg ttgcaggtgg cggattttta tttactatgt gttccggtac tgatacgtat
      301 gatattgcac tctctgcaga aggcttagat atttgtgaga gtatgtatga tggtgacggc
      361 actacaccta actacaatga tcatctggat ttttcaaaaa catttgcatt caggaattat
      421 actttaagtg ttaatccact tgaatatcgt ttttcgaata ttgatgtcac aacaacaaga
      481 agagtaccgc gcgatgaaga tgtgtttact ttatttgatt tttcagctaa gtgggaccct
      541 gttcctacta tgctttgtca ggaccacatg caggtgatta aaggttttat gggtcagaca
      601 actgcattca acaaaaatta tataaagcct gatgttatca ttatgggtga aaataaagct
      661 gctaatgaag ctaaatacat acatggagat tttggcaagg gcacttggac attttacgga
      721 ggtcacgacc cggaagacta tcagcacatg gttggcgacc cacctacaga cttgaattta
      781 catccgaaca gtccgggcta ccgactgatt ctgaacaatg ttttatttcc tgctgctaag
      841 aagaaaaaac aaaaaactta aaacgactta aggaagctat tcaggtgttg attaaatgca
      901 tctttatttt caagatgacc caagtgaccg gaattttcaa taatatgtaa aacacttccg
      961 ggaatttgct gctgcataaa ttctgatttt tccggaggtg ttaacacatc atattctcca
     1021 caaagaatca tggtcgggaa tttaattgaa gaaagattgc tgcatgactc ttctctttct
     1081 gccattgctt ccaaagtttg catcaacact tcattatcag ccttcatcat aaggtctaat
     1141 gtttgtttta ttactacatc gtttttttga aatgtggcag gtgaaaacaa aatcttacac
     1201 atatcctcga gatatttttg ctttccacct tttctgatta attcaatatt ttcatagcgc
     1261 tttgctttcc ctattgcatt atcggcatag cactgtgtat cgtataaaac aagtcctttt
     1321 attctttcag gatatcgttt gactaaattt agtgcaatgt atccacccat agataagcca
     1381 cagacaataa acttactcac ttgtaatgaa tcaagcaaaa aaattaaatc atcggcaaat
     1441 aaatcaatag aaaatctttc tttccctttt gaactgcttc catatcctct aacgtcatag
     1501 gtaattatgc gagcctgatc tgataaagca attacctgct ctttccagat tgactgatca
     1561 aatggatatc catggattaa caccagcacc tcattaccgg aagatgtatc ggaataattt
     1621 aatacttctc cgtcaggctt tattaatgcc tttgaataca tcattgtaac aggttgtttt
     1681 cttgttatca tcaggaaaaa ttacttcatt cagtgccttt gtaaattttg gccaacttat
     1741 ctttataatt ttcgagaata atttttctgc gcaagctaag ctttggtgtc atttcattgc
     1801 gttctatact aaattcacga tcgagcaact caaatcgttt aatcgtttca taatgaccta
     1861 gcgttttatt cactacttct acttcttctg caatgcgttt ctttacttca ttactggcgg
     1921 caatctctgc attggaattt actgcaatat ttttcttggc tgcccaatct ctgataaatg
     1981 caaaattcgg aacaataaat gctgatgcaa atttttgatt ctctccaatt accattgcct
     2041 gttcaataaa tcttgattcc tttaatttat tttctatcat ctgtggcacg atatattttc
     2101 ctcctgatgt tttaaagatt tctttcttgc gatctgtaat tttcaaaaac tttccatctt
     2161 caaaaacgcc aatatcaccg gtatggaacc agccatcagc gtcaattacc tctgctgtta
     2221 actctgggct gttgtaatat ccaagcatca cattagggcc tttgcacaat atctcaccat
     2281 ctgcagcaat ttttacttca acattttcga gtacagttcc tacagtgccg aatttaatgc
     2341 tgtcgggctg aaaattattt actgctataa caggacttgt ttcagtcagt ccgtaacctt
     2401 caagcacagg aatttttgca gcatggaata ctcgtgccaa gcgtggttgc agtgctgcac
     2461 cacccgaagc tattgcctga atatttccac ccaatgcctc acgccattta ctgaaaataa
     2521 gcttatttgc caagcgcaac tgaaactcat accaccatcc attagcacct ttaagttcat
     2581 aacgcaaacc cagattcaat gcccagaaaa acaaagcttt ttttatacct tttaactctg
     2641 ctccctttga aactatttta tcataaactt tctccagcaa tcgtggaact gttacaaaaa
     2701 tctgaggttt aatttctttt aaattgtcgg caatagtatc taaactctca gcataataaa
     2761 ttgaaacacc aaaataaaca tagagataag aaagcattct ttcatagaca tggttaagtg
     2821 gtaaaaaact taatgcgttc catgactcat gaaacgggca cagcacatcg caaaatttaa
     2881 agttgcttac aagattatta tgcgacaaca ttacaccttt tggatttcct gtagttccgg
     2941 aagtgtaaat aatcgttgcc aaatcatcag gctttatact atctcttatc tgcttaacct
     3001 cttcttgttt tggatgctta aggccaagat caatcaattc gctccaatgt ggtaaatcct
     3061 gatagcgatc gaataaatat atcttttcta acgcaggaag atcgttctta atttgactaa
     3121 ccttcttata taaatccaca ttagcaacaa ataaatagcg cactgctgac tggttcaacg
     3181 tgaattttaa atcattttca ctaatagtag gataaagcgg aacatcagca actgcaatct
     3241 gaaggcaagc atgatcaaca aaattccatt ccggtctgtt attggcaata attccgattt
     3301 tatctccggg tcttattcct gaatgtaata atccgtaact cagattattt attgtttcaa
     3361 ttatatcggc tgttgcatat tttttccata ctccatcctg ctttcctgcc aaagcatctg
     3421 ctttaggaaa acgtgaaagc tgatatggaa acaagtctat aagtcgtgtt acttccattc
     3481 ttcaattagt tttgttgtgc tgcataaaaa tataaaaagt atttaaatgt tttttactat
     3541 tgcaatatga actataatga tttattaaac aaatcaaagc cattattgat atttgatggc
     3601 agttgtgctt tttgcagatc atgtattcgg ttcgttaaaa aatatgacat caaacttcaa
     3661 tttgactttg cagacaacaa tcagaatacc accaaactta ttttagctca atactttata
     3721 aatcctgaat ctttaaactc cgttgttttg atagaataca acaaactaca catacagagt
     3781 gatgcaatta taaaaacctt acttcttctt ggtgggcgtt ggaggctttt agcccgaata
     3841 atgatggttt tcccaaactc cttcagaaat aaagtttaca atataattgc agccaataga
     3901 aataaattaa gaagtcattg cacagtatga aaaccaattt tacggagcaa acaggcatag
     3961 cctatcctat tataatggca cccatgtttt tagtaagcaa tgaggctatg atgcaatctg
     4021 caatacgaac cggcattatg ggtgtgtttc caactttaaa ctacagaaaa gaaggtgaac
     4081 ttgatgcatt aattaagcgg ttacatcaat tcaagtctga aaatcaaggg agtttcggtg
     4141 tcaatttaat tgtacaaaaa accaatcctc tctacaacag gcatctaaaa atatgtgttg
     4201 aaaacaaagt gcctttttac attacttcgt tagggagccc acgcgaagtt attgaagccg
     4261 cacattctta tagcgcaaaa gtttattgtg atgtaaccaa tattgcacat gcagagaaat
     4321 gcgctaaact tggttgtgat ggctttattg ccgttggtca gggtgcaggc ggtcatgcag
     4381 gcccttatgc tcaaacggta ttgattccaa ctttacgaaa acactttcct tctatacctg
     4441 taattgcagc aggaggtatt gccaccggtg acggcattgc tgcaatgata gctttaggtg
     4501 ctacaggagt aagtatagga acaagatata ttgcatctac agaagcagaa gtcagtgatg
     4561 aatataaaaa cagtatttta aattcaggta tggataatat tgttatgaca gaaaggcttt
     4621 ccggaacacc atgcaacatc atcaatacac cttatgcaaa aaaaattggc tataaacaaa
     4681 attggttaga aaagtggctg agcaataact ccacaacaaa aaaatatttt aaaatgatgg
     4741 tgcagtacaa aggcgttaaa gcacttgaac aagcaattaa accaggtaat tacaaaacat
     4801 tatggactgc agggcaaact gttgaactta ttgatgatat aataaattgt gatgaaatta
     4861 cagagcggtt aattaaagaa acccatctgt cggtcgaaaa ccttagtaat aaatttaagg
     4921 actaaaccta tccttctttc ttggggaaaa ggtgattgag gtaaaatgtc atttttaaag
     4981 cctcattaca ttcaatttca tgcttcgttt tcattttagt ttcagcaata atatatctgt
     5041 ttttcgttga attagcctac atttgaacaa aaaataaatg gcctttaaaa gaatattctt
     5101 ttttgtttgg ttttcagttt gcttccatgt tgcacatgca caaaaagtta cttatgcaca
     5161 acaggaactt gatgcctatt cttatgatta tataaaaaca gcaggctata acgataattc
     5221 cttttttgtt tttcaaagca atctgccatt tgaaaacgat cgtgacagag taggattcaa
     5281 atcacgtaaa tataaagtta cttgttacaa tctgaatcta tctttaaaat ggtcgaaaaa
     5341 aattgaacct aaaaatgata atgagaaatt agaaactata actgtgattg acggtcaggt
     5401 tgctttaatt aaatcttctt ttgaaggtaa ggacaatcaa ttgtcagtgt tcattaccac
     5461 attagatgac aatggaaatg aatctgctac caagaaagaa attggaacta ttttatttga
     5521 cagaacaagc aacttagata agataaaagt tattgcttcg aaagggaata actactttgg
     5581 acttattcaa aatgaaaaga aacaaaatgg tttgcagcag ctacattgcc tcataattga
     5641 tagtgacttt aagcttgtcc ggcaactgca atttgaagta ccataccctg aaaaaaattt
     5701 caactatgaa aactggcttt tgacagatga tgcagacttt attcttgaag gaagttacta
     5761 ctcaaaagaa aaaagtgaaa accgcaaacg atgggatgca tacaagttat ttgtatcgtc
     5821 caatggaaat agtggcgcca ttgaatatgc tatcaatacc agcaacctga tattaagcag
     5881 cagcaccata gcttatgatg ccataaataa gaaaatagtt cttgcggctt tttatactga
     5941 caatgtttct acaaaatccg gaataattta tgcaagtctt ggaacttcag gaaatgattc
     6001 gttgttgatg ataagacaac ctattggcga agtcacgtta acacgcatgt ttgccggtca
     6061 aaatttagac cgtagcagag caggaattga aaacctgagc atagaaaaag tgattttacg
     6121 aaatgatggt ggtgctgtat tagttacaga ggtgtcttat gttacagact actcctatta
     6181 tgattatttt acacaaaact acattcgtaa tttcgaatat catttcaata atgtcattac
     6241 cttttctatc aatgccgatg gtactattga ctgggatgca gttttaagaa aagatcagat
     6301 ttctacaaat gactatggaa aatattcttc gtttgcctta gccatgacag aaaatgatat
     6361 cagtttactc tataacaccc gcacagacag aaataatggt gttgcctatt tcggaataaa
     6421 aagaaatgga gaaacagaat cgaaagaaat catcaaacct gaagaacgga ttttactggt
     6481 gccatttgct gcaaaacaaa tcagcactga cgaaattgtt gtcccgtgct ggaacagaaa
     6541 aaagatgcta atggcaaaaa ttactttctg atgccgggat cattatctat tgcattgcag
     6601 tatatttctt attacgttaa ggcatggaac aaacacagtg ttcattctcc ttttgtatat
     6661 aagctcacaa cacaagttat tcatcccgac aaaaaacttg caaaatttat acctattgaa
     6721 caacaaagaa ttcttttaaa acaaagtaca gacaaaattt tttttaccga attaggtgct
     6781 ggaaataaaa gcaataaacg aagtgtttct gaaattgccg cgaagtcttt aaaatttccg
     6841 cgatatgcgc gtttacttta taggcttgca aatcattttc aacctgacaa tattattgag
     6901 ttaggcacat cattgggcat tacaacaggc tatatggcat tatcaggctg taaaaatatt
     6961 tttactattg atggtaataa aactgtatca gaaattgcgc aatctacatt aacctcgata
     7021 aagctgcatc aaaacataaa atttcttcaa ggaaattttg atgaagcact tcctgcatta
     7081 ttacatcctt taaataaaat tgattttgtt ttttttgacg gcaaccaccg ttatcaacca
     7141 acaattaatt attttcatca atgcttaaca aaagcacatg ataaaagtgt gtttgtgttt
     7201 gatgatatta actacagtca ggaaatgaaa caagcatggc atgaaatcaa acaacatcca
     7261 tcagttattg tcagtattga cttgtttatg atgggaattg ttttttttga tacagacctt
     7321 ctgcgtcaac attttgtaat caggtattaa catttggtaa cttaatcata agccacaatt
     7381 tattactttt accacgtaca aatcaattat gccacagtcc attatttcgc tttcagatat
     7441 ttcacgactt tataaagtcg gaacagaaac aatttatgca ttacgatctg tttcacttaa
     7501 catttttaag aatgaatatg tggcattgat gggtagttcc ggttcaggaa aatccacgtt
     7561 gatgaatatt cttggttgcc ttgacacgcc atcaagtggc agttatcatc tgaacaacat
     7621 ctctgtttct ggaatgacag ataatgaact tgccgaagta agaaataagc aaataggttt
     7681 tatttttcaa acatttaatc tgttgccacg aagtacagca cttgagaatg tagcattacc
     7741 gcttgtgtat gcaggtgttt caaaagaaga gcgtttaaag cgggctaaag aagtattgga
     7801 aagtgttggc ttggccgaca gaattatgca taagccaaat gagttatccg gtggtcagcg
     7861 tcagcgtgtg gctgttgcaa gggcattggt caaccaccct tcaatcattc ttgctgatga
     7921 gccaacagga aatcttgatt caaaaacctc tgaggagatt atgttgctat ttgaagaaat
     7981 acatcagcgt ggcaatacaa tcattgtggt aacccatgaa gaagacattg ccagacatgc
     8041 acaccgtatt gtcagaataa aagatggact cattgaagat gatgccatca acacacaaat
     8101 aacaaaagca aaaacaattg tatcatgaac atcaaatcaa ttcaatcgct acttatagtt
     8161 gtagtgctat tagcaagttg tggagtagca aacagtcaga aaggaaaact cctcacacct
     8221 gaagtatttg aaaaaatgct tcacgaaact ccggatgcac aagttataga tgtaagaaca
     8281 cctgaagaat ttaatgatgg gcatctgaaa aatgctttga acatgaatgt caacagcaaa
     8341 gattttgaaa accgtgcacc ctatctcgat aaagaaaaac ctctttttgt ttattgctat
     8401 tccggtggtc gcagtgccaa agcctgcgaa tattttgaga agatgggatt caaagtcatt
     8461 tatgatatgg aaggcggcta ttcttcatgg acagatgcaa acctgcctgt agaaggaaaa
     8521 aaagctgaca aggccggtat cagtcttgat aactttgcct tgcttacaag tgccgggaaa
     8581 gttttggttg atattaatgc gccatggtgt ccgccatgtg taaaaatgaa acctatgtta
     8641 gattcattag ataatgcatg gaaagacaaa gtaaaaattg tgcgattgaa taaagacgaa
     8701 aatcaattga tttcaaaatc gcttcagata aatgaattgc ctaccttact tttttatgaa
     8761 aacggtaaat tggtgaaacg tgaaagtggc tataaaaatg ctgaacaact aaaggctatg
     8821 actcaggttg aatagttgag tggatttact ttttattcta cttttaaatg agattgcatt
     8881 agaataattc attaataaat cccgaatatt aaaaacgcct tctctgctaa atcatattga
     8941 cctatctcac tgattcagaa cacatagaat taatattttt ttagtcttgc cttctccttt
     9001 tcaaaagtgt aaattgcgct ttttcagaca tataaaacgt gtaacctaaa gggcataact
     9061 acgtataaag tcatttagtt gttgagcaaa ttatgaatat atctgcatta gcagaagccg
     9121 aaaaacttct atttattgca aggcagtcgc tttcagaagg gaaactgccc caagcaatag
     9181 atatgctcaa taaaatcgtt accgactatc ctgatttttc aaaggcacac atcactctgg
     9241 cagatattta ctttaataaa cttcaagact caacaactgc agaagaatat tataaaaaag
     9301 ctattgccat tgccgatatg aatactgacg cctttaccgg ctacgcaaac ttaatgctgc
     9361 aacaggaaaa atatcccgaa gcactgtcaa tgctcaacaa agctatggcc ataaaaggtg
     9421 ctccaaaaga tactatctac atgctgctgg gcaaactcta cgaaatgcaa ggcagactcg
     9481 atgatgctat tggaaactat aaaaaatgta ttaccactac cctatcaaac tccgttcttg
     9541 aagaagcaga acttgcattg aaaagatgtg aggtcaagaa aaagtatatt taattttttc
     9601 tatttacaac gttgttttta gtgctttgca acctattcta ttctgcttta caacttacag
     9661 atagaattta aaaaccccat ttcagggtaa gccaaaaatt ttcacgaata cattacattt
     9721 gcctttaaac tacaactgta aattaatgaa acgtgtcttt accatttcac tgtgtttttt
     9781 tgtattttat tctataagtt ttgctcagtt agaccaatat gcttctgaac agaataaatt
     9841 ttattggaaa aatcgtaaac ctactgctgg ctactggcaa caggatgttt attataacat
     9901 taaagctgca atagacgaaa aaacagacat tatcagtggt gtagaagaac ttacttacag
     9961 aaataattct cccgacactt tgaaatttat ttttatgcat ctctatcaga atgcatttgt
    10021 tcccggtggt tacttgaacg acttaaacaa agccaatcaa aacaaagtgc gttatggaaa
    10081 atatgaattg caaggattgg gaattgaaat tactaatata aatacacttt ctgtcaatgg
    10141 tattataata aataaacaaa ccgaaaaaat aattgacaac accatcatga aaattatgtt
    10201 gccgcaacct ttgatgccgg gtcatgatct tgttttgaaa atcgatttta aaacttatta
    10261 cgataaaacc ggtcagcgca gacgcttaca gatgtttgaa aatgcaggca acaaacatta
    10321 caacggatgt caatggtatc ctaaaatgtg tgtttacgat tcgcatcgtg cttggaacac
    10381 cgatcagcat ctgggcagag aattttatgg agaatatggt gtttatgatg tggaactaac
    10441 ctttgcaaac aactttatag tagaggcaac aggtgtttta gtcaatgaga aagaagtttt
    10501 acctgatgaa ctacgcaaga aacttgatat taaaaatttt aaagaaagac ctgcttcacc
    10561 acaaccttca acaataatca tccctgacgg aagtacgaaa acctggaaat tccatgcaga
    10621 aaacgttcat gattttgcct tcactgccga tccttcctat cgtctttctg atgcttactg
    10681 gaatggtgtt cgttgtgtag ctattgcccg cgagcaacat tgctacggat ggcaaagtgc
    10741 ggcagaatat acagcacgta ttattgaagt tttctcacgc gattttggaa tgtacgatta
    10801 tccgaaaatt gttactgccg atgcaaatga tggaatggaa tatccaatga taacaatgga
    10861 caatggtaca gatcccggat tccgtggcct gctatgtcat gaaataggac ataattggtt
    10921 ttatggtatg gtaggaagta atgaaacata caggccattg cttgatgagg gatttgcgca
    10981 gttgctgaaa tcgtggggta tggaaatact tgaaggctat catataaaac agagagaaac
    11041 aacatggaaa gatcagaaaa gaacagagcc tttaacctat cagatgacat cagcatacgg
    11101 cctctattac aattatgttc taaatgataa agatggattt atcaatacac atagtgacgg
    11161 tttcagcagc gcattaggtc atggtggtgg ttatggaatg gtttattaca aaacagcaac
    11221 catgttatac aacctgcaat atattttagg tgacagtttg tttttaaaag caatgcaaca
    11281 ctatgttgca caatggaaat tttgtcatcc ctatcctgaa gatttcagaa acagcattac
    11341 agaatatgtt aaaaccaatt taagctggtt ttttgatcag tggatggaga ccaataaaaa
    11401 attagactat aaaatctgca agtatcgcaa agccgatgaa aaagacgctt ttaatgttta
    11461 ttttaaacgc aaagggcgca tgcaatcatc aattgatttt tctgtctatt caaactgtgg
    11521 tgtaaaatac gattatcata ttcccaataa ctggtttgta aaacaaaccg atgcaacggt
    11581 attgccgaaa tgggaaggtt gggataatct gagaaatact tataaggctc aaattaaaat
    11641 cccatgtggt attagcagta ttgttataga cacgacagac cgtttaggtg atgcttatag
    11701 gttggacaac cgcttgccgc gaccttttaa acttgtgttt gataaatggc agaaacctgc
    11761 ctactcagac tggaaaagct atgtatttta cttacgtcct gatgtttggt tcaacaatta
    11821 tgacggacta aaagttggtg tacatgcaga aagtaattac atgaatacca tgcacttttt
    11881 ttcaggtgat gtttggatta atacaactgt gggacaaaat agtttgagcg aaactgccga
    11941 cataaataaa aatgacccag catcattcag aataaattac aaaacaccat taaagtttgt
    12001 tgcaccggga gttaattttt ggattggtgg cggactgctt gatggtttaa catttgcaaa
    12061 atcaggaggt gatttaactt tcctaaacaa tcgtgtaaga ctgtttgctg aatacaaatt
    12121 gatgtacaga agaaacacca acacgcttga atatttatta tacccgaatg aatggcagtc
    12181 ggctttgatg aatacatctg taaccttagg tgcagaggca caatacagaa aaaataaatg
    12241 gaacggttct gttgtcatac aaaacacttc agcatcatta tttagtgatt actcctattc
    12301 taagctttca ttaactaaca tcacaaacta cgaaacagga aaaattgact ggcgattaag
    12361 agttttcggt caatatggat atggacaaaa tgttcctatg gaaagtgcat tatatctagc
    12421 cggtgctaat ccggaggagt tgatggaaag tgcttggaca cgttcacgtg catttgttcc
    12481 atacagttgg ttgggctatg gagacaagac caatcacttt cagcaaggtg gtggacttaa
    12541 tttaaggggt tatgcaggtt atcttgtgcc gcagaatatt gatggcgaac aaagatattt
    12601 ttatcgtgcc atcagtggtg cttctgttag tttagagata gactttgaca ggttgattcc
    12661 tttacgccca tcatttacta aaaagtggtt aaaagctgac ctttattttt ttggagatgc
    12721 aggtgccatt gcaacaaaca attctaacaa gtccattcaa ttcactaatt tgaaaagtga
    12781 tgcaggcatt ggaacagcat ttacaattaa aaaatttggt cgtttgaatg ccataaagcc
    12841 gctaactata cgatgtgatt ttccattctt gttaagcagt gccccttatg ctgaaaaaga
    12901 ttatgtcaaa ctacgatatg taatcggcat cggtcgcact ttctgataac atcacgctat
    12961 tgactgaaaa gttctatttg ctgacttccc gattcaagtt gcagcaactt ctcctttcgc
    13021 cacaaaccgc ctgcataacc ggttagtgca cctgaagttc caataactct atgacaagga
    13081 acaattattg caatcggatt tttaccattg gcagatgctg atgctcttat ttttttcgca
    13141 ctccctaact gcactgcctg ctcgttataa gagatggttt ttccaaaggg tatagaaagt
    13201 aacttctccc aaacctgaag ctgaaatact gttccctgtg gttttaaatt taaggtgaat
    13261 aacttacgtt taccggaaaa atactcttgt agttcgtttg caatctgtat tgacacagct
    13321 ccgttcaata tctcaccgga agtgtatttg tcatcaaatt caagtgcaag cacactatct
    13381 gtatctgtga tagcattcat atagcctaac ggtgtcagga tatacgaagt atataattca
    13441 gttaacaata atggcaataa ttgattttta caaattgaaa atccctaagg ctttaccttt
    13501 ctgatttcag actgcaaaac ataatagttg ttggtctgat aaacataggc cagaatataa
    13561 cactgatcaa cattatactt atcaggaatc tgatagttgt aaagtctgat atactttgtt
    13621 gtcagatccg gatcgtttat caggctttca ccaaaagaac tatttaatgc agctctaagt
    13681 acgtgtctgt gtatatatgc tgaatctatc tgcgtaggtg ttgtcaaatc ataatcgagc
    13741 tgacatccgt gaatactgtc ttcaactaaa aatactgcta acttataatc atcgccaagg
    13801 ttgttcaaaa actcagtact tactgtaagt tgagctgtgc gattactttc actatatgca
    13861 gaataaatat ttataaaagc atcattcggc ttacttaact cttgtgatat aacagactgc
    13921 caactgttgt agtatttaat atgtgttgtt ggatacccta ctctgttaat catacttgaa
    13981 ggatatgcag ataaaccaaa aaaagtattc aactcatcgg agttggaatt tctgaaatct
    14041 tcagaaaagc aacttggtgg attatcagga actgcgtaaa caccggcatg gttggttgca
    14101 acaaccaact gttcatggta taatggctca agtgtgttag caattattct ctgtgcacgc
    14161 ggacagtttc cacacttacg tcctgtataa tcttcgagta acactttctt aacaggattt
    14221 gcaggggtgt atggaaccac aaaatcagcc tgacctgtta gcgtatcgct aatagtggct
    14281 gaatatttaa atgtcagtgt tccaaaaggc gcaggagctg ttatgtaaac cgaatcatta
    14341 tcaaagatgg catcattgcc tgttgccact tctgaaatca cgttagattg tgcaccgtgc
    14401 catgtaaact gaactgcaat agttttaggc gatgatgatt cgagtgaaaa cttagctaca
    14461 agaacatcta catcgtcagc tgttgtggtt gcattaagca ctctgatgtc attgagtgtt
    14521 cctggaagtg taccaatgtt agttgtgttt tcttctttat aaggaccatc aactttatcg
    14581 catgaaacaa ttgttgttgc agctaaagct gcaattgcta accaaaattt atttttcatt
    14641 ttcgtgaact tttaaataat taaaaacttg tacttattga aactgtaagt ccattacttg
    14701 acggaacctg tctgcacaca ccaccaacgc aaaacaaacc ttcgcgctga cgaccatagc
    14761 ccaaagtaat acgatgcgaa tcttttgtgt atccgacaaa acaattgaag taatggattc
    14821 ttttgtcaga tttttcattg ccataattat actgatctga tactgcaaca aaccaattgg
    14881 gtgagtgtga atactcaatc agtccggcaa tccaagactt ataatcttgt tccgattgta
    14941 atgtttgcca ctctgttctg atggcgtttt tatcatttaa ctgaaaggta atatcagcta
    15001 ctattatatt gtcataaata gttccatatt gctttgatcc ctgaaactga attttgtctt
    15061 tatcataaac ctgattaata tacattaaag taattttcag atttttagac atcttacgag
    15121 taatctctaa gctaaaatct ttgtagtaaa catccttacc aaattttccc caatcacttt
    15181 tataaaggtc tttgcctgca acaggctctg tgctcaatga attggctccg gaatagttaa
    15241 caatgatatt tgttccataa tcaccaccta attttgttcc tttcttaata gtatagttta
    15301 cttcagcttc aaatccaaac tcaccattcg gttgtgttgc ataaggatag attgatgcta
    15361 atgaatacgt gtgatatttt gtcaatgcag gaagataatt aatcaataag tcatttagat
    15421 ttgcattacg ctcactacgg aaattcatgt tgtcaattct tttggcggca agtaatattc
    15481 ccaaaccttt ttgagaataa gttgctttca aaagtgcggc atcaccaaac ttatagctgt
    15541 aattatttaa tgatgaaggg tcgttgtatt tataagcata ctctgcattg atggttactt
    15601 tacctcttgt aacgtcaagt cgtccggcat aagcaccaac gttctcaggc aagatatata
    15661 cattgtcatc atctttctga tacttactca caaatccaaa acctgcagta accctggtct
    15721 ttttatcatt ccattttttt attaaatcat ttatacccac ttctgcatct actcctctga
    15781 caatgccttc tccggtttta aaataatagc gttgtttacc aattatacct ttaataagga
    15841 ttccactgta aggtttaaat tttacacgaa caccatccaa tgcattatca taacctagtg
    15901 ttggctccca ataggaacgc aaaatcaatc cactgccaaa ctgctcataa aaatttccga
    15961 cagtaatagc taattcgcca ttgtcatatt ctgcataacg atgcgcaata ccactgccat
    16021 taaatctttt gtcgtagcca agcaatacat tctgataagt ttcgtaacga acacctgctt
    16081 tgaaattacc tttggtaaaa accaaatctc cgtaaccatt aagcaaatat ttctctgcta
    16141 cttcaggtgc acttatagtt gaatcctgaa tgtaatactg tccatcaatc tgaaaactac
    16201 catgtatttc accgggagaa ctttgtgcaa gcgataaagt aggtgcacct aaaaaaataa
    16261 ccggcaatat tctgccggtt ataagtttta tcattttatt catgaaaagc aaatagttat
    16321 ttattaattt gattctttag taagtgcttt aatctcttcg tacattttat gttcatcacc
    16381 gggtgcatag cctgttttta ctgaaacaat ttcgcctttt ccgttaacta agaaaacata
    16441 aggtactgct ccaatgttga gggctctttt cagatcaccg ttggggtcaa cataaacttc
    16501 atagtcccaa cctttgccat tgacaaaagg tttaacgttt gccattgttc gtgcatcatc
    16561 aacactaatt gctacaattt taactcctgt ttctttctgc cattcctcgt aatgttcact
    16621 gatggctgta agttcattca cacaaggctt acaccatgtt gcccaaaaat caagcaccat
    16681 cggtttgcca ttgttattta atgttgacgt gtttacagtt ttaccattca gatcttttac
    16741 atcaacagca ggtattggac gtgattcaaa aacctgtgcc gaagaaactg atactgcaca
    16801 tgaaaagact actactaact tctttaaatg attcatttat ttattaaaat tgaattttat
    16861 ctgtttacta ctaacttttg tacagctgaa ctttttcctt ctacattaag cgaaataaaa
    16921 taaacaccag atggcaaatc ggctgttgaa agtacaatat ttcctgttga gctatttaca
    16981 ttaaaattct taactgtttt tcccaacata tttattactt taacttcagc cttgctacct
    17041 gatttaatat tataattaaa tgcagttatc gaacgtgcag ggttaggaga tggcgcactt
    17101 atgctatttg ttttatttac ttcttttacg ccaaccggtg taatatgata gttaatggca
    17161 aactgaactg aatcaagtgg attgtttcca tcaaatacag tataggctac agtagtagta
    17221 ccaacataac catgtccaag ataatcacct ctgaatgatc cttctgttgc acctgcatta
    17281 atagtagcag gtattgcaga tgacatcaca cctactccgt aacataaaat accccaacaa
    17341 aagttatgct ctgttccggg aaccttgtaa acatcatagg cattcaggct ggcatctatt
    17401 gtattctgac tgttattaat tacatataca taagaatgta ataaactgtc ttcaacagag
    17461 ccataaacat ctgcagataa tcctgtatag ctaaaacttt gagctttaac caaacttgct
    17521 gatgtaatta aaattagtgc tagtacaatt ttcttcatga tatttttttt aaagctgccc
    17581 cgttatctta gataccggga cagcaattga tttaattatt taattgttat tttttgataa
    17641 gaaactgaat cgcctgaacg tacttcaagt gtataaattc ctgcagcaaa atcagaagtt
    17701 tttacagttt catttactac agaaacagtg ccgagattct tctggaacaa ctttcttcct
    17761 gaaatatcag ttaaggaaat tgtcattgga agtgatttct ccattgttgc tttgatattc
    17821 aatacatcac ttgcaggatt tgggaacact tcaagagtag ttacaccttc tatctgatga
    17881 acagctgtta cacctgttaa attaatatca tcaacataaa ggttgttacc ataagcatta
    17941 gttgctttga aacttaaaag taaagatggt tgacctacat aattagctaa tgaaaccgtt
    18001 ttagattccc attgagatgc tgaagttggt gtaaatgctg tggtttgtgc tggtgaagtt
    18061 gataatgctg cacctgcttc attaaataga gatgtccaag ttactccaca atcagttgat
    18121 gcaagtacct gaagacgatc gttctccgtt gaatattgac aatatgctac actgaaagtc
    18181 aactgaggat tcacagcatt agtcatatca attggtgtat aagggtaaag atagtcagat
    18241 tgaccagctg cagcataagt ataaaagttc atttttgcag aaccacctgc taagctaccg
    18301 gcagcacttc taacccatgt agttgcattg tcagggttat ttagaatcca atttacagga
    18361 ggaaatgttg tagcaagaaa atcctgactg agtgatgtta ccggtgctgt tgtttgtgca
    18421 accgatgttt tataatcaag atttatatta tggaaagcag cattaccatt aacgctgatt
    18481 aatttagctg aaaaattctg taatccagca cctggcaaag ttaaattagg taaagttaat
    18541 actacagatg tatttgatcc caaattgcca ctccaattat aagtttgcat tgtttgtcca
    18601 actataccgt attcaatgtc cgcagaagta aggtcaccac ctcctccata attttttaat
    18661 gtgacactgg ggtcaattga actggtacaa gtaaatattg atgcaagatt tgtaatagcc
    18721 gccaaaggca ctactgctgc agcactactt cttccgcctt gcataatttc tttagtggcg
    18781 tcattctgaa caaaagctac agcacgaagc attggccaat atggcataaa cactgtaggt
    18841 atattagtgg gtacatttat tgtccattgt tcatccaaaa caactgaatc accaacagcc
    18901 caagttgccg gcaatattgt tcctgtgcca ttaggcaaca ttttacgcat tacatcagaa
    18961 taatgagatt caccatttgg cgaagtataa ccatataagt ccttttcagt aatggcaata
    19021 cgtgctttta aacttgttaa tcctgtcaca gcctgtgtgg cacgaattac ggtatgagta
    19081 taaataatat cgtgagtagg tgaaaaacta tgtgtaacat caactgtaaa aggtgaagtt
    19141 gttgctacac gtgagtttaa tgttgcaaca ggaaatgaac ctgcattacc atcccataca
    19201 ttgccatcta aaaatgcatg tggtgagtag ttatttgcat aaaacgatgt tctgtttgct
    19261 acatcaatag tattatacga ataataatta ggcccgttag atggaatatt attctgatac
    19321 ttaatcggaa tagtaaatgc attagcattc aatttggtgt tgaaaccggg attattagct
    19381 gcacaaggcc cacaagtttc acctgtgaat tcttcaacaa ctacatatct ctgtgactgg
    19441 gcatttgcta tacccactac taagcacgct gccgttaaaa tagtaaagat ttttttcatt
    19501 attataaata tttgatttta aaattcttgg ataaaagtac acacttttca gaataccaat
    19561 gtctaagaaa tgccattttt aataaactgg taaaatcaga ccgcaagtga gtgtttaaag
    19621 ctatggttat tacagcgcat catggcaaat tggtgtcacc tgccattggt ctttactaca
    19681 aagttgtgat tcaggctttt ggtcggtcaa aatcataaat ggaatattag ttgtgagcgg
    19741 gcctttttta aatggatttc ttaaaaaacc tgccttttta agccattttc cctcttccga
    19801 cttcggaatt gcaattgtta tgattccctt cagcttgctt ttaatacaaa tttgtgataa
    19861 tctcctgatt aaagttttct gcaccttaac agatatacta caataatcaa gtaaaaacaa
    19921 cttcccttca tcttcataac aaataacata acccacagga ttttgtcctt gataacaaat
    19981 aaaacaccga taattaaatc gtggattagc caatctccat tttaaaaact ctttgctgta
    20041 atgttgtttt atattttttt taaagccgaa attcttttga atatcaagaa tcacatgaat
    20101 atctgttttc tcaactatat taaaatttct atcttctgtg gtaaaattat taatccattt
    20161 gcaatgcaat gcatgaataa aaaagtattt cacatctgca atatgacacc atcccatctt
    20221 caccaatccc ggagttgatt tgtcgcctcc aaaaccaatt actgctaact gatgatgctc
    20281 cctaacatag ttataacata aggttgccaa tttctcaaac agcccctttc ttctttgtgt
    20341 tgagtgagtc atagtatctc cggattgaaa tattattact tcactattat ttatctgata
    20401 ccgttccgga atgacaccgt agaaaccgac caatttattt tcaaagaatg caccaaaacc
    20461 aataaaattt cctgccggat tatctaaata cttccattta aaataagctt tgtcaacctt
    20521 catactgaag cagttctcca tcagtccggg caacaaccct atttcatctt tcagaagctg
    20581 cctaagttga acttcactca ttttataaag cccttttcgt aaagtttaat tctgacattc
    20641 atataactat tcggctcttg aatcatgttt aatctgttgt aaaatctaat tttatctgta
    20701 gaagttcctg cattacttcc cgatccagcc aaacagagta ctgttttata accagcttca
    20761 gatattttct gattagcctc gccatttgat aaaccatttg gcaaagctat aacaggacat
    20821 tcgcatttaa gcatttcttc aattcttttt ttggaataat ttaattcatg ctcaaacgtt
    20881 acatcattct taacagccga aagactaaca tgagatgcag tatgagaacc gatttcaaca
    20941 ttatttgtag ctataaactt aatatcatcc cagttcatca tctttatcgg gtaatcagtt
    21001 tctgtaatat tcagatactg ctctaaaact gcaatacatt cagaccgatc tattacatca
    21061 agtttaaaca gataaaataa agttttataa taatgagcga accaattttt gcaaggttgt
    21121 ttaatatcga gtacaaagtt tttgtctaat gcaatgttta ttgaaaagcg gctgctattt
    21181 cgcaacaagt taaacagatg attcagtttc tgtgtccaga tataactttt accatcggca
    21241 caatctatca caatgttgtg attgcttggc gcctcataat aatgtaacac tggtattgct
    21301 tcttctataa aatctttgta gccatcatcg aagcttaaaa ccaacggtgg cttggtgctt
    21361 ctgaaagatt tattttctat ctgactaatt gggataattt gatattcctt aactaaagac
    21421 tccagcatca aacgaaaggt ttcaggataa ataggctgcc agaaataatc gtattgtgat
    21481 gaaattcgat ggaaacaaag tattgtcaca ccatttggtg aagcgggaat taacttcagc
    21541 aaattttgaa gttgaagctt tacagcagtt ttaaagatga tttgtgttga aaaagacata
    21601 acccttgtcg ggctaaggta taaaaaagag atttacaaca aactatctac ctcactcttg
    21661 agtgataaaa ttgccgcccc tgcaaaattg ttgcctgctg atgaacgttg caaaataatt
    21721 tgactcagca caattgcctt ttcggtcttg tccaaagaat ctgcggcaag atttactgtt
    21781 gcaattatgt catcttttgc ttttgtgatt aatgaccagc tttgtacaga aacatacact
    21841 tgctgactaa cattatgttc gtactcctga cgaatggcag caagtaaatc aacctgaagg
    21901 tctcgcacat tcatgccggg ttgtaaaatc ctgaataaga tattctccgg ttgtatgcgt
    21961 tccagaaata caaccattct ttctactgcc tgaagtttta atggcagcat atctttttgt
    22021 atcagtcgtc ttgcgtcaag aagtttaagt ctgtaatcgc gttccaacat attttttatg
    22081 agcatataca tgccaccggc aacgataaaa gttggcgcaa tgatgaggag tgctgttgtt
    22141 aaattatcaa agttcatttt tagaatccta tattaaatcc accgaaaagt gttgttgttt
    22201 taccattttt tccataataa aatggtgcag tataaacgtt agccatagca cctttattat
    22261 catcaatttt aacacctgca gataaaaaaa tgtcattaaa taattcgtat tttatattca
    22321 aatcaagtga tgtaaactca tattcttttg tttttaaaaa tggcagaccc aaaccagatt
    22381 tatctttgcc tgtataggga taaataggcc caacggctgc tgaggtgtat cctgctgaaa
    22441 tatataaacc tgaaattggc gcgcaacgtg caccaagata aatttcctga gcattgtcgc
    22501 gaagataatg tcccatatta aatctattag attcgtaggt ggtggtattc acaaagtgtc
    22561 tgtatgtaac cggatttgtt cttgtatatt ctgcaataaa agtagctgaa gtataaaacg
    22621 gtaacattac agcagcgcct gctttaagac tgtaaaagtt tgaatgctca tttttatcca
    22681 tcattcgccc aaatgcaatt tcatcaacaa atagtgaaat ataaagatgc gtatatttaa
    22741 tattgtgtga actgatgtta aaaaacaatt gagagttttg acctgaatag ttactaccct
    22801 gccctgtttt actatgatca agcgatttaa aaaacataaa cggaataaac atggcaactt
    22861 ccagttgatc gctataaaca atagaattac caaaactgaa atcgaattgt ttaaaaggct
    22921 ttacggtgaa catgttggca gcaaaatatt ttgaaaaata tacttgtctg tggttaggcg
    22981 gattgcctga gaaataagct gcagaatcat ccttaatttc agaataaagc catccgtgaa
    23041 aatagttgaa ctcaaaccac ttttttggat taagcttaag ttctaatcga gcaaaagatg
    23101 gcgcctttgt tgaaataatg tttgcaccat gattattact gccccattca aaattatctt
    23161 ttactattcc aacatgaccc cacttccaag cataggcaat gccaccacgg gtttcatcaa
    23221 agcttcctcc acgtttaggc gagccaacag cacctttata tttataatta tatccaggca
    23281 agtcgttcag atatttatca tctgcaactg ctctactgtc agtataatcg cgatagcttc
    23341 caaaaatact tagccctttt cctaaagttg cccagaattc tccacctgca tattggcgat
    23401 acatattgtc attttcattt ttataataac tgaatccgcc aactgggtta actgtaatat
    23461 taaacaacga gtctttaaaa taaaacaaat ccaaacgttt tttaaatccc ttacctgtaa
    23521 ataattcttt attaaaatct tttaaataaa atgcaagttg tttttgctgt gtcgcattta
    23581 atttttcttt ttgtgattcg gccgtttgta atgcctcggc aatttgtttt ctggaatatg
    23641 gttttattgc atcgttaagt gtgatgatat gaagattagc taagtcatcc ataaaatcat
    23701 aaagtggttt attgaatact gactgaggta catactgcgc agatatattc aattgaaatg
    23761 caaaagacaa aatcaaaata aaaaccaaac ggcttgcttt cataaaattt taaattatga
    23821 ttgcgcaaag atgaaaaaaa taatttaagg aatacttgtc cgctatttgc ttctggcacg
    23881 cttgaaaaga atggtgtaaa acaccattaa gaaataacgt acatctgtca ggaatgggct
    23941 tttctgatag gcttttaaat agcgcatttc tgaatccata atttcttcca ttgttttagg
    24001 taggtcaaca tagtatggag gaattagtcc gggtttgaac tttttacgca tgtcctgaag
    24061 ttcctttgaa tacagactta aataatgtct gctcaacggg cgaacaccaa caatctttaa
    24121 atcgccttta agtacattca aaatcattgg caactcatca atccaatatt ttcgaagcat
    24181 gcgaccaaca cggcttaccc tgaaatcgtc tttaaactta ccgccctctt cgaggttgtt
    24241 tttttcataa acatattcct gcagatattc tgcataggca tgcatggttc tgaatttgta
    24301 aacgctgata atttttccgc ctttaccaac gcgtttcatc ggataaatga caccataact
    24361 tgggtcttca tcataagcag gatccctgaa cttttctgaa acaatataaa gtctattacc
    24421 tatataacgc tcttctataa ctttaaatcc gcatgaataa agtcttccca acatttcagt
    24481 cttagacaac acgcgattat gcccttttgt gattgcataa taaatttgct gtgttaccgg
    24541 tagctttgga aatacccgtt taaaaacaaa gtcaaagaaa taataaactt tgtttagata
    24601 tggtggatat ttttccatca gacgttgttt gcgcaggtcg tttgtttcga ccttactgac
    24661 aaaaatccca cctttaggta acttggtatt gattgcctcg aattgtttat ttatgaattg
    24721 aaaattattt actcttttaa agtgaataac tgcatggtat gtttcatcag ccaattgatt
    24781 tgcatcatcg tgattgttag ctgcaaaagt gaagcaatca ctacgtgatg tgtcaacgta
    24841 tttttcaatt aaattaaaga caaacctttc attacggaca tcatttttgg ccttagtagc
    24901 caacactgca tccagttcag caaactcctc taactgcaaa agttttgcct ctgtgttatc
    24961 ggttttaaaa atcaatactt aaaaaaagtt gaaaaatgtt taaaataatt cgattttggt
    25021 ttgctaagat aagcacgaat ttcgaattgt gcaagtgtat gcttcaaatc gttttaaaca
    25081 aaattcaatc aactatttaa ggctttttaa gatgttattg agttaaaagt ttgatcataa
    25141 attggtttaa atcaatacat tgattaagca tcattgaacg tttttcctgc cattgcagtt
    25201 ttaaattctg ttgatttaat aaagtttcaa cgtgaacaag catggcatct gcatcgctag
    25261 ttttaaagcc aaacgttaaa gcaaactctt tttcaagttc ttcaaggtaa ctaatctgac
    25321 caacaaagtc attaaacctt atggcaggtg ttcctaaaac agcagcttca gcggccatag
    25381 tctgactgtc gccaatatac atctgagcaa agtaaagcgc atcatgaata tgcaacgggt
    25441 ttattgcaat acgatattgc tcaaactgag attctaactc tcgctctgaa gtgatataaa
    25501 ccctgccatg cttagaaagc atatttatta atttctctgc aattagagta tttataccct
    25561 tacggccttc gtcatgatgt gctgttagtt ttgcaaaacg aagtataaaa aaaggttcat
    25621 cccctaaatt ttttatgttt tcttttttgg gcgagaaatt ggacggatgc agatatgcca
    25681 attcatgata tcctttatag ccgatttttt tatgttccca cttacctaca ctgcaacaat
    25741 aaggtgcaag aatatttgtt gcccagggat aagctaattt tgcaaaccat ggaacaactt
    25801 ctgcatcgtc ttcattaact acaatacttc tgatgccgag taattttcca acatgtgcaa
    25861 tttcagccga agtaccaatc attatctgtg gcttaaatcg aagacaaatt tttaaaaact
    25921 gaaaatcgcg tttaagtaat tttaatgcaa tggaaaattt atcatccctt ctgccttcag
    25981 gattgatgtt tatgtattgc atacctgcat gtctcaacaa atcttcaaga atatcttttt
    26041 tcttgatgag aatcattgct tcatgacctg attgctgtaa tgcaataatg gtatttttaa
    26101 acaaatgaaa atgggccgga tgacccatat aaaacaagta acgcattatc taaatcagat
    26161 gcctttaata actgtattaa ttttttgtgc caatgatttg ctgattgatg caaccggaag
    26221 cctgaaataa ttctgacaga tatctaactg cgacaaaaca tatttaattc ctgaagggct
    26281 accttcggca aacaataatg gtattaagtc aagaagttgg taatgcagtt ttctggattc
    26341 agtaatttta ccttgtagcg caagtcgtac cattgtactg aactgctttg gaaaggcgtt
    26401 tgcaataaca gaaattacac catcggcacc tgcagctatt aacggcattg taatagcatc
    26461 atcaccggaa ataactaaaa atcctttgtt tctatgctga atgagataca ttatctgctc
    26521 catattacct gaagcttctt ttataccaat tatgttttta caacttgcag caatttcaag
    26581 cgttgtttgt ggcaatagat tacttcctgt tctacccggt acattataaa gaataatagg
    26641 caactgagat acacctgata gcgttttgta atgctgcact atgcctgact gatttggctt
    26701 attataataa ggagaaaccg aaagaatggc atctgccata cgggcatcta tttgttctaa
    26761 ttcacgtgca gtttcaagtg tgttgtttcc ccctactccg aaaacaatat gtgttctttt
    26821 attattaacc tcactaataa attttaaaat atctttcttt tcttcttttg aaagcgtcac
    26881 cgactcacct gtagtaccca ttacaactaa atactcaaca ccacctttaa taacatgatt
    26941 aactaccttg cgaagcgcat taaaatctat tttgccattt tttaaaaaag gagtcactaa
    27001 tgcaacacct gtacctgtga atttattcat ttatcgtgtc caatatgttt gagataatta
    27061 tttacgtggt ctataaactg tggtagtgta acctgaccat taacactaat cataaaatcg
    27121 taaagccccg catacccttt ttcataacga ccgattttaa aacctgcttt tgttgcagca
    27181 ctgatatatc ttaacggcat gcaacgatct acattaagac aaatcagata atcaaaatcg
    27241 agattaacaa acttggaaat cataggatgt gaaggcttga aatgccaatt gagatttttc
    27301 ttggtaaaaa aatcaagtcc aagttttgaa aatcgcattg gcggcagttc attgccatca
    27361 taatatccaa gtgcaagaac atctttctta aaatcgtcac gaagatgttt tacataggct
    27421 tttacaattt cataattacg ctcaatagta gcatcataca aaataccaat agtgcgcgca
    27481 ttactgaaac ttgtggtgcg tggctgatga tgctgacgct gcaattcacg gtaaaataaa
    27541 tagcttgcag ctttttgttt tattgcctta cctattttca ttgaagcatt tttaggaaat
    27601 catcttcaga gataatgttg attttaagtt tctctgcctt ttcaagttta gcaggtccac
    27661 agtcgctacc tgcaagcaga taacttgtct ttgagctgag cgagcctgaa acctttcctc
    27721 catttatctc aattgtctct tttaatttat cgcgctcaaa tttctgaaat actcctgtaa
    27781 tcacaaaact taacccttta agtttactgc cagcctcttg cattgcagct ttatcaagtt
    27841 taaattgtaa tccggcactt tgcagttgtt ctaccatttt aatgttctct aactctcgta
    27901 caaagtcaaa aatacttgtt gctattttat ctcctatttc cggtgtctgt atcaattctt
    27961 ctaaagtggc ctgactgatt ttttgatatg aaccaaaatg acgtgccaat ttttttgctg
    28021 ttgtatcacc aacaaatcta atgcctatgg caaagagtac acgttcaaaa ggcacttgtt
    28081 ttgatttttc aattgcttcg aataaattgc ttacagtctt ctcccccatt ctttcaagtt
    28141 gaagtaactt ttcggattta agatgataaa tatcagcaac gttttttagt agttcggctt
    28201 caactagttg gtcaattgtt tcaacaccga gtccatcaat attcatagcc ttacgtgagg
    28261 tgaaatgcac taacctacct tttatttggg gtgggcatcc ctcttcattg gggcaataat
    28321 gattagcctc cccttcaagc cgaataagtt ctgtaccaca ctccgggcaa tgtgtaatgt
    28381 aactgaccgg cttggctcct gcttgacgca ttgagaaatc tactccggta attttaggaa
    28441 taatttctcc acctttctct acaaaaaccg tgtcgtgcat atgtaagtca agtttctgaa
    28501 tctgatcggc attatgcaat gaagcacgct taaccgttgt gcctgcgagt aaaacaggtt
    28561 ttaaattggc taccggtgtt attgttcctg tacgcccaac ctgatataca acactattga
    28621 gaatggttga agctgattca gctttgtatt tgtaggcaat tgcccattta ggcgattttg
    28681 cggtaaaacc caattcttct tgctgacgga aattgttaac tttaataact atgccatcaa
    28741 tatcaaaagg aagtaaatct ctcttcttcc gccaagattc aataaaagtc aaaacatctt
    28801 caatggattt gcatagttga tagtgttcag aaattttaaa tccccaatca gcagccattt
    28861 taagtccgtc ataatgtgta gaaaaatcaa aatcaccata aagtgcatag agaaaacaat
    28921 cgagtttacg tctggcaact tcactgctgt cttgtagttt gagggtgccg gcagcagaat
    28981 ttctcgggtt ggcaaaaggt gtctcgccaa tgtctgcacg ttcagcatta attctttcaa
    29041 atgttttttt atcgagaatg atttcacctc ttatttcaaa cttatccgga aaattgttgc
    29101 ccatgaggct tagaggaatt gagcgaatag ttcgaacatt agttgttaca tcatcgccac
    29161 gaacaccatc accacgtgtt acagctctaa gtaattttcc gttctggtat gtcagaccaa
    29221 tagctacacc atcaaacttc aactcgcata catattcaaa atcgctacca attaccttcc
    29281 tgactctttc atcgaaatcg cgcagctctt cttctgagta agtattaccc aatgacatca
    29341 tcggataact atgagtaacc gttacaaatt ctttagagat aaatcctcct acacgctgtg
    29401 atggcgactc ggtagtcagt aagtttggaa actgtttttc cagagctatc agttcctgaa
    29461 gcataacatc aaattcataa tcactgatga cgggttgggc ctttacgtag tataaataat
    29521 tgtgatgatt aagatcaacg gtcaattgtt taatcttttc tcctgcttct ttttcagaaa
    29581 ttttactcat ataccttaat gatgcaaaaa taagaatagt tgtctaaaga
//
LOCUS       DAOOOL010000012        28859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_125_length_28859_cov_196.930565, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000012 DAOOOL010000000
VERSION     DAOOOL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_28859_cov_196.930565"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..333
                     /gene="rplW"
                     /locus_tag="PKU82_02065"
     CDS             43..333
                     /gene="rplW"
                     /locus_tag="PKU82_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOZ81688.1"
                     /translation="MSILKKPVITEKMTQKSEKLGQYAFIVDKNANKIEIKAAIEKLY
                     NVNVVSVNTVNNAVKSKTRFTKAGILQGKKGGQKKAYITVAKGETIDFYASI"
     gene            344..1168
                     /gene="rplB"
                     /locus_tag="PKU82_02070"
     CDS             344..1168
                     /gene="rplB"
                     /locus_tag="PKU82_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOZ81689.1"
                     /translation="MAIKKFNPITPGQRFLIAPDHSNITTNEPEKALTERINRKGGRN
                     NSGKMTMRYIGGGHRKLYRIIDFKRNKDGVPGKVATIEYDPNRTARIALVHYADGEKR
                     YILAPTGLTVGQTILSGDKATPEVGNAMMLKDMPLGTIVHNIELRPGQGGKMARSAGS
                     YAQLTARDGKYAILKMPSGETRMVLSTCVATIGSVSNSDHNKESAGKAGRNRWKGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGKKTRKLKNQSNKHIIERRKK"
     gene            1214..1486
                     /gene="rpsS"
                     /locus_tag="PKU82_02075"
     CDS             1214..1486
                     /gene="rpsS"
                     /locus_tag="PKU82_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOZ81690.1"
                     /translation="MSRSLKKGPFIDPKLEGKVASMSEGKKKTVIKTWSRRSLISPDF
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFAPTRTFRGHAGNKDKK"
     gene            1486..1926
                     /gene="rplV"
                     /locus_tag="PKU82_02080"
     CDS             1486..1926
                     /gene="rplV"
                     /locus_tag="PKU82_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOZ81691.1"
                     /translation="MGARKKIAADARKEEMKSTYIARLNNCPTSPRKMRLVADMIRGK
                     KVDLALGLLKFSKKEAAGNMYKLLLSAINNWQAKNEGVRIEDQPLVVSKVYVDSARQL
                     KRLRPAPQGRGYRIRKRSNHVTLLVDTVNKKVENTKQVTENTEA"
     gene            1927..2694
                     /gene="rpsC"
                     /locus_tag="PKU82_02085"
     CDS             1927..2694
                     /gene="rpsC"
                     /locus_tag="PKU82_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOZ81692.1"
                     /translation="MGQKAHPIGNRLGIIRGWDSNWYGGKNYSDRLVEDEKIRNYLHA
                     RLAKGGVARIIIERTLKLITATIHTARPGIVIGKGGSEVDKLKEEIKKITKKEVQINI
                     FEIKRPELDAQLVADGVAKQIEARISYRRAIKMAIASSMRMGAEGIKIMCAGRIGGAE
                     MARTEQYKDGRIPLHTFRADIDFAIADAQTSYGKIGVKVWICKGEVFGKRDLSPNVGM
                     NSTPKQNAGGGDRRQERERGGNDRGGRRGGRPRGEKK"
     gene            2726..3148
                     /gene="rplP"
                     /locus_tag="PKU82_02090"
     CDS             2726..3148
                     /gene="rplP"
                     /locus_tag="PKU82_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOZ81693.1"
                     /translation="MLQPKKTKFRKQQKMRNRGNATRGSQIAFGSFGIKSLENAQLTS
                     RQIEAARVALTRFMKREGQTWIRIFPDKPVTRKPAEVRMGKGKGAPEYWIAQVKPGTM
                     LFEADGVSVETAKEAMRLAAQKLPVITKFVIRRDYSAA"
     gene            3168..3368
                     /gene="rpmC"
                     /locus_tag="PKU82_02095"
     CDS             3168..3368
                     /gene="rpmC"
                     /locus_tag="PKU82_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOZ81694.1"
                     /translation="MKQSIVKDLTTEEVKSRIKEEVDNLQKHKMQHAVSPMDNPMKIR
                     STRKLIARLKTELNKRMSQTSK"
     gene            3377..3637
                     /gene="rpsQ"
                     /locus_tag="PKU82_02100"
     CDS             3377..3637
                     /gene="rpsQ"
                     /locus_tag="PKU82_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOZ81695.1"
                     /translation="MSTERALRKTKIGVVTSIKMQKTVVVAEEKKFKHPMYGKFINKT
                     KKFHVHDETNACGSGDRVLIMETRPISKSKRWRLVEILEKAK"
     gene            3688..4056
                     /gene="rplN"
                     /locus_tag="PKU82_02105"
     CDS             3688..4056
                     /gene="rplN"
                     /locus_tag="PKU82_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOZ81696.1"
                     /translation="MIQSESRLTVADNSGAKEVLCIRVLGGTRKRYASLGDKIVVTVK
                     NALPAGNIKKGAVSKAVVVRVSKEVRRNDGSYIRFDDNAVVLLNQADELRGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            4074..4391
                     /gene="rplX"
                     /locus_tag="PKU82_02110"
     CDS             4074..4391
                     /gene="rplX"
                     /locus_tag="PKU82_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOZ81697.1"
                     /translation="MSKLKIKKGDIVKVIAGESKGKQGRVLEVFPETNRASVENVNIV
                     SKHTRPNAQNTQGGIVKKEAPVNVSNLMLIDPKSGKPTRVGRKTEEGKLVRYAKKSGE
                     VIK"
     gene            4391..4948
                     /gene="rplE"
                     /locus_tag="PKU82_02115"
     CDS             4391..4948
                     /gene="rplE"
                     /locus_tag="PKU82_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOZ81698.1"
                     /translation="MSYTPRLKGKYKKEIISALKNHFQYKSIMQVPKLSKICLSQGIG
                     DAVSDKKIVDACVAEMTAIAGQKAVATYAKKDISNFKVRKGVPIGVRVTLRDEQMYEF
                     LDRLVSVSLPRIRDFRGINNKGFDGKGNYTLGITEHIIFPEINIDKVSKIRGMDITFV
                     TTAKTDNEAMQLLKEFGLPFRTGSN"
     gene            4965..5234
                     /gene="rpsN"
                     /locus_tag="PKU82_02120"
     CDS             4965..5234
                     /gene="rpsN"
                     /locus_tag="PKU82_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOZ81699.1"
                     /translation="MAKESIKARSRKREHLVEKYAAKRAALKEAGDYQALALLPRNSS
                     PVRLHNRCMITGRPRGYMRQFGISRVLFRKMALEGKIAGVTKASW"
     gene            5265..5663
                     /gene="rpsH"
                     /locus_tag="PKU82_02125"
     CDS             5265..5663
                     /gene="rpsH"
                     /locus_tag="PKU82_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOZ81700.1"
                     /translation="MTDPISDYLTRLRNAVKANHRIVEVPASNTKKEITKILFDKGYI
                     ANYKFEETDNKQGNIKVALKYNPVTKLPAITRLKRVSRPGLRKYVSHDRLPRIINGLG
                     VAIISTSKGIMTDKEARQLHVGGEVLCYIY"
     gene            5675..6229
                     /gene="rplF"
                     /locus_tag="PKU82_02130"
     CDS             5675..6229
                     /gene="rplF"
                     /locus_tag="PKU82_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOZ81701.1"
                     /translation="MSRIGKLPVAVPSGVQVNVQGNTVSVKGPKGTLSQTIADGITLE
                     MEENKVVVKRSTEQKRHKSLHGLYRSLLANMIKGVSQGYKIEQELVGVGYRASNQGQM
                     LDLVLGHSHHVVFELPTEVKVSTKTERGSNPMIILESHDKQLIGQVAAKIRSLRKPEP
                     YKGKGIKFVGEVLRRKAGKSAAKK"
     gene            6243..6593
                     /gene="rplR"
                     /locus_tag="PKU82_02135"
     CDS             6243..6593
                     /gene="rplR"
                     /locus_tag="PKU82_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOZ81702.1"
                     /translation="MKNSKDSRRLKIRKRIRGKIKGTNEQPRLSVFRSNKQIYAQIID
                     DVAGQTLAAASSAENGAAKGTKVEISTSVGKMIAERATAKGITKVTFDRGGYLFHGRV
                     KALADGAREGGLKF"
     gene            6603..7121
                     /gene="rpsE"
                     /locus_tag="PKU82_02140"
     CDS             6603..7121
                     /gene="rpsE"
                     /locus_tag="PKU82_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOZ81703.1"
                     /translation="MIATANKRVKPGEIELKDRLVAVNRVTKVTKGGRTFSFAAIVVV
                     GDEKGVVGYGLGKAKEVTDAIAKAIDDAKKNLVKVTVHKTTVPHEQDAAFGGAKVFLK
                     PAAPGTGVIAGGAMRAVLESVGIHDVLAKSKGSSNPHNVVKATITALAMMRDAVAVAN
                     QRGVDLNKVFNG"
     gene            7132..7314
                     /gene="rpmD"
                     /locus_tag="PKU82_02145"
     CDS             7132..7314
                     /gene="rpmD"
                     /locus_tag="PKU82_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOZ81704.1"
                     /translation="MGKVIIKQVKSGIDRDKNQKLTLKALGLRKVYHSVEKENTDQIK
                     GMIEKVRHLVEVTEIK"
     gene            7325..7777
                     /gene="rplO"
                     /locus_tag="PKU82_02150"
     CDS             7325..7777
                     /gene="rplO"
                     /locus_tag="PKU82_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOZ81705.1"
                     /translation="MNLSNLKPAKGSTKKSKRIGRGEGSGHGGTSTRGHKGAQSRSGY
                     SRKFGFEGGQMPLQRRVPKSGFKNFFRVEYTGINLDVLQKLNEKFGLTEIDHSILCEK
                     GLIAKRELVKILGRGELKVKMNVKAHAFSETAKNAIEAAGGTTTIQEK"
     gene            7777..9138
                     /gene="secY"
                     /locus_tag="PKU82_02155"
     CDS             7777..9138
                     /gene="secY"
                     /locus_tag="PKU82_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOZ81706.1"
                     /translation="MKELIQTLRNIFKIEELRTKLLNTLFFIAIYRLASHIVLPGVDP
                     QQLGNLKNQASQGIFGLLDMFAGGAFSQASIVALGIMPYISASIVVQILGIAVPYFQK
                     MQKEGESGRNRLNQITRWLTVLVVAGQAPGYLVNLRAQAQSAIVAYSNPDAMSPTLWW
                     ITSIIILIAGTLFVMWLGERIQEKGIGNGISLLIMVGIIARLPFALRDEFLSRLNQTG
                     GGLIMFLVELVALFAVVVISIMLVQGTRKIPVQFAKKIIGNRQYGGVRQYIPLKVNAA
                     GVMPIIFAQAIMFIPATVAQFFPESSSMQGIAHSLTDFHSVWYNVTFAILIILFTYFY
                     TAITVNPNQMADDMKKNGGFIPGIKPGKQTASFIDDVMSKITLPGSIFLAIVAILPAF
                     ATILGTSTNFAQFYGGTSLLILVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRTSIN
                     APV"
     gene            9147..9971
                     /gene="map"
                     /locus_tag="PKU82_02160"
     CDS             9147..9971
                     /gene="map"
                     /locus_tag="PKU82_02160"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOZ81707.1"
                     /translation="MVQIEYRSKEEIDLIRVSSLLVGKTIAEVAKHLRDGITTGELDK
                     IAEDFIRSHEAVPAFKGYHGFSGSLCISVNAEVVHGIPGARVLHPGDIVSLDCGVVKN
                     GYYGDSAYTFAIGEVDQQAVKLLQVTRECLYLGIEKAVAGNRMGDVSSNIQKHAEANG
                     FGVVRELVGHGIGKKLHEKPEVPNYGKAGQGLLLKPGLVIAIEPMINMGTKNVKQLND
                     GWTIVTADQKPAAHFEHTIAVDNVKAEILSSFEEIERSEKENLNLYKIDYKQHIIA"
     gene            9985..10203
                     /gene="infA"
                     /locus_tag="PKU82_02165"
     CDS             9985..10203
                     /gene="infA"
                     /locus_tag="PKU82_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOZ81708.1"
                     /translation="MAKQTSIEQDGTITEALSNAMFRVELENGHEIIAHISGKMRMNY
                     IKILPGDKVKIEMSPYDLTKGRITYRYK"
     gene            10237..10353
                     /gene="ykgO"
                     /locus_tag="PKU82_02170"
     CDS             10237..10353
                     /gene="ykgO"
                     /locus_tag="PKU82_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOZ81709.1"
                     /translation="MKVKPSLKKRSVDCKIVRRNGKLYVINKKNPRFKQRQG"
     gene            10399..10776
                     /gene="rpsM"
                     /locus_tag="PKU82_02175"
     CDS             10399..10776
                     /gene="rpsM"
                     /locus_tag="PKU82_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOZ81710.1"
                     /translation="MARIAGIDLPRNKRGEIGLTYIFGIGRSTAQQILDECGISYDKK
                     VDQWNDDELNKIRSFINDNLKIEGALRSETQLNIKRLMDIGSYRGVRHRLGLPVRGQR
                     TKNNCRTRKGKRKTVANKKKVTK"
     gene            10829..11233
                     /gene="rpsK"
                     /locus_tag="PKU82_02180"
     CDS             10829..11233
                     /gene="rpsK"
                     /locus_tag="PKU82_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOZ81711.1"
                     /translation="MAKQQQASAAKTAKKKVVKVEATGEAHINATFNNIIISLTNLQG
                     QVISWSSAGKMGFKGSKKNTPYAAQLAAADCAKTAFDLGLRKVKVFVKGPGAGRESAI
                     RSLNASGVEVSEILDLTPIPHNGCRPPKRRRI"
     gene            11281..11886
                     /gene="rpsD"
                     /locus_tag="PKU82_02185"
     CDS             11281..11886
                     /gene="rpsD"
                     /locus_tag="PKU82_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOZ81712.1"
                     /translation="MARYIGPKTKIARKFREPIFGPDRYFEKRSYPPGMHGAAKKRAK
                     QSEYAIQLAEKQKVKYTYGMLERQFARFYSIALRKEGITGENLLKLIESRLDNVVFRL
                     GIAPTRAAARQLVGHCHITVNGKVNNISSYILRPGDVVGVRERSKSFEAITDSLASRS
                     NKYGWLEWDTTAMQGKFVTTPAREEIPENIKEQLIVELYSK"
     gene            11951..12943
                     /locus_tag="PKU82_02190"
     CDS             11951..12943
                     /locus_tag="PKU82_02190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOZ81713.1"
                     /translation="MAILAFQRPDKVIMNHSTETFGQFEFRPLEPGYGITVGNSLRRI
                     LLSSLEGHAITNIKISGVDHEFSTIKGVVEDVTEIILNLKQVRFKKQIEGTDSEKVQV
                     VVNGKTEFTAGDIAKHTSAFQVLNPELVICQMDPSVKLVIDMTIDKGRGYVPAEENKS
                     NSHPLGTVAIDSIYTPIKNVKYAVENYRVEQKTDYEKLVLEITTDGSIHPKEALREAA
                     EILIHHFMLFSDEKITLDTKSKTPVEEFDENTLHMRQLLKSKLVDMDLSVRALNCLKA
                     ADVETLGDLVSFSKTDLLKFRNFGKKSLTELEELVRSKNLTFGMNIQKYMLDKE"
     gene            12969..13451
                     /gene="rplQ"
                     /locus_tag="PKU82_02195"
     CDS             12969..13451
                     /gene="rplQ"
                     /locus_tag="PKU82_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOZ81714.1"
                     /translation="MRHGKKINHLGRTHSHREALLSNLASSLILHKRINTTVAKAKAL
                     RKYVEPLITKSKSDSTHSRRTVFSYLQNKEAVSELFREVAPKVNERPGGYTRILKTGF
                     RPGDAAETCMMELVDFNELMPSSKEKGTAKKAKTTRRGRKPKAKADAAGSETTEATEE
                     "
     gene            13512..15155
                     /gene="bshC"
                     /locus_tag="PKU82_02200"
     CDS             13512..15155
                     /gene="bshC"
                     /locus_tag="PKU82_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOZ81715.1"
                     /translation="MFTVIFSDSTNMFVQNTSIPFADAGYSALITDYLNNNIFLKEYA
                     SFQYDESGFKEALQKRVKYKANRDRLCEVLEQQYSETIFQTDWKTDKSYSAIKSLKEE
                     NTFTVTTGHQLNIFTGPLYFIYKILTVIKQAEFLAQRNPGYKFVPVYWMASEDHDLAE
                     VNHIDIETSKIEWQTNQKGAVGRMTTEGMYSVVEALKNQLNKNDNFQELATILDTAYL
                     KNATQANAIRSLVHSLFSKYGLVIIDADDKRFKQQAIKLFEDDLFNNTSFNCFKNLSD
                     FEKAYGLQIHAREINLFYLVNGDRSRIIKNGERFEVADSGLSFSSDELKKHINEYPER
                     FSPNVVLRPLYQEIILPNIAYTGGPAEVHYWLQLKSIFDKLKVFYPMVVLRNGMMVVN
                     ADQATVLNESALEWKDLFHPTEQVVNIALKSKYGEELSLKEEKDTLQRSYEEMEQRFK
                     AIDITLEKHVKAEWRRTEKRLMASEKKLLRAFKKKQVDETCKIRNTVKQLFPNGKLQE
                     RKENVFPYLSKYGLQFIDDLYKICEPLPTNFKIAVTAEK"
     gene            complement(15162..16337)
                     /locus_tag="PKU82_02205"
     CDS             complement(15162..16337)
                     /locus_tag="PKU82_02205"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOZ81716.1"
                     /translation="MDAYIVAGYRTAVGKAPRGKFKNTRPDDLAVTVIKHLIQSVPNF
                     DTELIDDVIAGNATPEAEQGLNIGRMISLMGLNTVKVPGMTINRYCSSGLESIAIASA
                     KIHSGLADCIIAGGVECMSPIPFGGWRIVPNYEVAANNPEWYWNMGLTAEAVAKEFKI
                     NREDQDQFAFQSHQRAINAIKNNLFKEQIVPVEINETYLDANEKRQTHKSTVDTDEGP
                     RQDTTIEKLNKLKPVFDAKGTVTAGNSSQTSDGASFVLVVSEKMLKTLNVKPIARFVS
                     YAVSAIEPRIMGVGPVYAIPSVLKRAGLKQNDMELIELNEAFASQSLAVIRTLGLNPE
                     ITNVNGGAIALGHPLGCSGAKLSVQLLNEMKRRKMKYGMVTMCVGTGQGAAGIYELL"
     gene            complement(16395..17039)
                     /locus_tag="PKU82_02210"
     CDS             complement(16395..17039)
                     /locus_tag="PKU82_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81717.1"
                     /translation="MKILKQLLLLLILLPAFAFAQREEKPRYAPGREKHRNKTDELGQ
                     RQGVWKYYNEAGELNQEIEYLDDVRNGIAKKYYPYGKIMEEIEYANGIKDGEYKRYYY
                     NGQAKMEGEYLNGKKSGTWTTYFSDGEIRNTGPYKVNLKNGLWKVYDRKGKLISQVEY
                     KMGEDAKALAAAKEADEKAKKAAAAKKAADDKKKLPPGAKLKTPSPPPSTPEKK"
     gene            complement(17244..19649)
                     /locus_tag="PKU82_02215"
     CDS             complement(17244..19649)
                     /locus_tag="PKU82_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOZ81718.1"
                     /translation="MRRKIEKVAVLGSGIMGSRIACHFANAGFNVLLLDIAPKEFTEA
                     DKKAGFVEDSPKFRNKITDTHLQSAIKSNPSPLYHKSFAQRISIGNFTDNLKDIANFD
                     WIIEVIIEDLNIKRTLFEQVEKFRKPGTLITSNTSGIPIHLMTEGRSDDFKKHFCGTH
                     FFNPPRYLPLLEIIPTQFTDKDVIDFISHFGDRYLGKTTVLCKDTPAFIANRIGVFGI
                     MSLFHSMEKTGLSVFAIDKLTGPIIGRPKSATFRTSDVVGLDTLVHVANDAYKNLPDD
                     EQKNLFAIPPFINKMIENKWLGDKTKQGFYKKTKTAEGKTEILELDLTTLEYKPSAKI
                     KFKTLESTKTVDDLKQRLPMLLSGTDEAASFYRYMFFSLFSYVSHRIPEIADEIYKID
                     DAMCAGFGWEIGPFETWDILGVKSVVEQMQKEGYSISPWVTEMISNGINSFYKIDNGK
                     KSYFDLQTKKLNQLPGEGSLVILKNTSNEKIVWKNSGCLLHDIGDGVLNLEFKTKMNT
                     IGSEVLQGINTAIDFAEKDFKGLVIGNEAANFSAGANLGLVFMLAVEQEYDELDYAVR
                     AFQNTNMRLRYSNVPVVVAPAGLTLGGGCEMSLHADKVQAAAETYIGLVELGVGLIPG
                     GGGTKELTLRFSDSIKPGDIETNALRDFFMTIATAKVSTSAFEAYDLHILRKGFDNIT
                     LNRKRLLSDAKNAVLSLANAGYVSPQPRHDIRVLGRQGLGVIQAGASAMLAGNYISQY
                     DQMLSLKLGYIMCGGNLSSPSLVSEKYLLDLEREAFLSLLGEKKTLERIQAILTTGKA
                     LRN"
     gene            complement(19758..20261)
                     /locus_tag="PKU82_02220"
     CDS             complement(19758..20261)
                     /locus_tag="PKU82_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ81719.1"
                     /translation="MPKTIFLIRHSKATREYSQINDSDRPLTEKGCHDTEKMSLILKD
                     HINSVDLILTSTAVRAYASALIYSKHLGYHPNNIQLEKNLYMTGSKDMLAILKSLDDQ
                     INSVALIAHNPGLERLVKFLTGTESLHFSTSGLAMIESKIDAWNELNAGNCNLLSYRH
                     PKETIYN"
     gene            20442..20927
                     /locus_tag="PKU82_02225"
     CDS             20442..20927
                     /locus_tag="PKU82_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ81720.1"
                     /translation="MLKFLSIAQSKKRENKQVLKDLSKSKSPQLDNLFREESDNFFKK
                     FSCLDCANCCKTTSPIFRDVDIERIAAHLKLKPGDFVAKYLHLDSDSHYVLNHSPCPF
                     LGTDNYCSIYTVRPQACREYPHTDRKNINQIMDLTYQNTLVCPAVASIVEKLKTDFIK
                     K"
     gene            complement(20942..21292)
                     /locus_tag="PKU82_02230"
     CDS             complement(20942..21292)
                     /locus_tag="PKU82_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81721.1"
                     /translation="MKQKQKKSNNLSKVKNFDFDHYLNLRKAERIKLLQQMVEKLDPA
                     SRKNFFKSLLQYVLSDEELTALAFKNNSGKQEVKKSRVVELSDKEKEMLGQIADYFHE
                     LELIMDLDELLINP"
     gene            complement(21359..23140)
                     /locus_tag="PKU82_02235"
     CDS             complement(21359..23140)
                     /locus_tag="PKU82_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ81722.1"
                     /translation="MDTPVVKWIIRKLMLDSIYRSIQDDDILAKQRFNLFRIFNLVAI
                     IGIGLICYQAIYVFQTEKNLAYILLGLESLLILNYLLLNVHKNLTLSVVISLLSCFGA
                     LHYITYFAGGIRNSGMFYQIAFILISFMLLGSRYGIWFTILSVANMIYFFLITEYTNW
                     IDYSFIGETASLINQDYLITGLVSLIVISSMINNLESKKNVVISTITDSRNKLAIINK
                     ELKKLSLVASKTDNAVVITNENGVIEWVNDGFTRITGYQFYDAIDQSLQTFLSGPETD
                     KENLEAVQNSMREFKFCDSELLRYKKDGTTVWMHASITPISDNEKNSRKFIFIMSDIN
                     ERKKAEIQLSEYLSDLEKTNKELDKFAYIVSHDLKAPLRAIGNLTDWITQDSADSLSD
                     ESKENFQIIKGRVKRMEQLINAILEYSKASKHKGTQELFSFNEIVHNAVDLAGADSNC
                     KIEISGAMPEYYGDKVKYQQVFMNLISNGIKHNNKEQKHIEISCHEEGKFWEFKIKDN
                     GPGIDKRYHEKVFVIFQTLKARDEFESTGIGLSIVKKIVEEAGGSIRIESTPGEGSAF
                     FFTVPKNQIDASLITGKNNQQISPLVL"
     gene            23249..23674
                     /locus_tag="PKU82_02240"
     CDS             23249..23674
                     /locus_tag="PKU82_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ81723.1"
                     /translation="MNKQLTILLIDDDKVDSMIIKRAIAKLDFPYQVDTAHNGLDALM
                     MLRGMSDIRIKRPDIILLDINMPLMNGLEFLSELRADPELKDIHVCVLTTSEDDSDVK
                     TAYEFNVAGYFIKPLNISDFDDRFLYLNGYWQRNCFPVQ"
     gene            23823..24884
                     /locus_tag="PKU82_02245"
     CDS             23823..24884
                     /locus_tag="PKU82_02245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ81724.1"
                     /translation="MDSQTRYIPHLDGIRFIAAMMVVFNHYLSELNFDYKFSTGFYGV
                     QIFFAISGFLITGILLRLKNAEKVIPRRLIIKNFFAKRFLRLFPPYYLLIFSYIFLEC
                     FGLYIAQKGWIVFYLAYLPNWFFYFHGWQGTIDNHLWSLGVEEQFYLLWPFVLLLLPK
                     GTEFKWIITMIITGYLFKCVIRQYFMLGGDPFLLPISQMDTLGIGALLACGFEYRMQF
                     IEYFKKALPYLLPLAILLTLVTSYDKPRNDPFLCLSLILLSSSLVLNAVVGFKGVVKI
                     FMENKWVVYGGKISYGIYLYHKVIPTLFISSVERVGIGMPNRWLTLVVSIVLTFLISH
                     LSWKLIESPILKLKKNFEH"
     gene            complement(24881..26155)
                     /locus_tag="PKU82_02250"
     CDS             complement(24881..26155)
                     /locus_tag="PKU82_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ81725.1"
                     /translation="MENQEQKSKFQQAIDVITGVAKITIGAGIVHCKAQDEQMDGRYI
                     TLEGQKKLFFGTCGYLGIEKHPKIIEAAVEALHSHGAQFSSSRAYVSSHYYEEAESLF
                     SKMFGRPALVMQSLTLGHITGLPLLVGDNDAIIMDVQAHDSIQSATAMLKLRNVKIDI
                     VRHNRMDILEDRIKVLKNRYQKIWYLGDGVYSMHGDFAPVKELYALADKYEQLHLYLD
                     DIHGMSWTGPHGTGMVMNAVPYYHRKLFLSTGMTKAFGTAGGLLTYPDEEYFKLVKTC
                     GKGFIFSIQLPPAILAATIASCKIHMSDEIIPLQQSLMAKIDYFNKKAEELGLLVIRK
                     ERSPVFFIGVGNPNVGYSTVLRMKKAGFFFNLSVFPAVSIKHTGLRITLSNHIQIEDI
                     DELLETLAENLPVVFKENNTSFEQIKEEFANH"
     gene            complement(26234..27394)
                     /locus_tag="PKU82_02255"
     CDS             complement(26234..27394)
                     /locus_tag="PKU82_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOZ81726.1"
                     /translation="MKQQVGIAGAGSMGSGIAQVAATNGNIVTLFDTNIEALQKAKAS
                     IRSSLDKLVEKNKIEKTLSDSIFSQITFTDNLNELKSSDLFIEAVIENIEIKQSLFKS
                     VESIVSDKCILATNTSSLPVIAIGSTCNQQDRIIGLHFFNPPVLMQLVEVIPSFNTSE
                     KVLTNSVEIIRQWSKTPVIAKDTPGFIVNRIARPYYGEALRIYEEGIADFATIDHAMK
                     TIGGFKMGPFELMDFIGNDVNFKVTETVFTQMFFDPRYKPSITQKRLYEGKLYGRKSN
                     RGYYDYKNNIQPKPSLDNNLHSQIFDRIFAMLVNEAADALMMGIATAVDIETAMTKGV
                     NYPQGLLTWANKRGLRAVYNKMEELFAYYGEDRYRVCPLLRKLALNDEMFRI"
     gene            complement(27391..28170)
                     /locus_tag="PKU82_02260"
     CDS             complement(27391..28170)
                     /locus_tag="PKU82_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ81727.1"
                     /translation="MESTVLQEIKGQVVQLTLNRPEKFNSFNRVMSLQLQDELKKAAN
                     NSDIRAILITGTGKAFCAGQDLSEAVDTNGPGIAAIVREHYNPIITLIRNIEKPIVCA
                     VNGVAAGAGANIALCCDIVLAAESAVFIQAFSKIGLIPDSGGTFFLPRLIGFQRASAL
                     TMLGDKVSAAEAVSMGMIYKSVDDALLMETAMQISQTLANLPTKALGLTKKLLNQSLS
                     NNLNTQLECELEEQVNAAKSYDHSEGIKAFLEKRKPVFKGQ"
     gene            28285..>28859
                     /locus_tag="PKU82_02265"
     CDS             28285..>28859
                     /locus_tag="PKU82_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81728.1"
                     /translation="MESNFATPSNLVSDSVSAFWQVHRSKESALHETIIPKGVIEIIF
                     NFETTQLQAQVNNRHITVPRCFIQGYSTSPIHLHLDGSQTFFGVVLNPAAVKYIFNFY
                     PQIFANCVIDLTLIDSTFCFLWHKLGEQNNFNNRVQVFTDWLINRLPELNEREKAFNC
                     FLNSDSNIQLSVTQFASNLENQTCRFLFRLR"
BASE COUNT         9558 a         4939 c         5572 g         8790 t
ORIGIN      
        1 ggaagaaggc gctttgccgg taattgaaaa aatattaaac aaatgagcat cttaaagaag
       61 ccagttatta ctgaaaaaat gacgcagaag tccgagaaac tcgggcaata tgcgtttatc
      121 gtagataaaa acgcaaacaa aattgaaatc aaagctgcta ttgagaagct ttataatgtc
      181 aacgttgtga gcgtaaatac agttaataat gctgtaaaat caaaaacccg ttttacaaaa
      241 gcaggtattc tgcaaggtaa aaagggcggt caaaagaagg cttacattac tgtagctaag
      301 ggcgaaacaa ttgattttta cgcaagcata taaaaattga acaatggcaa tcaagaaatt
      361 taatccgatt acaccaggtc agcgcttttt aatagcgcca gatcattcta acataacaac
      421 caacgaacct gaaaaggctt tgacagagcg tattaatcgc aaaggtggaa gaaataattc
      481 aggtaaaatg accatgcgct atatcggtgg aggtcatcgt aagttatata gaattattga
      541 ctttaaacgt aataaggacg gtgttcccgg taaagtagca actatagagt acgatcctaa
      601 ccgtacagca cgtattgcat tagttcatta tgctgatggt gagaaaagat atattcttgc
      661 tccaacagga ctgacagttg gtcaaactat tctttctgga gataaagcaa cacctgaggt
      721 gggcaatgct atgatgttga aagacatgcc attaggtaca attgttcata acattgaatt
      781 gcgccccgga cagggaggta aaatggcaag aagtgccggt tcttatgctc aattaacagc
      841 tcgtgacgga aagtatgcaa tcttaaaaat gccatcaggt gaaacaagaa tggtgctttc
      901 aacatgtgtt gcaactatcg gttcagtttc aaattctgat cataataaag aaagtgccgg
      961 aaaagcagga cgtaaccgtt ggaaaggcag aagaccaaga gttcgtggtg tggccatgaa
     1021 ccctgttgat cacccgatgg gtggtggcga aggtcgtgca tccggtggcc atccgcgttc
     1081 gcgcaaaggt ttgttagcta aaggaaagaa aacccgaaag ctgaaaaatc aaagcaataa
     1141 acatatcata gaaagaagaa agaaataatt ctccataatt aataatacgc ttcaagctaa
     1201 taataaaaaa taaatgagtc gttcactaaa aaaaggcccc ttcattgatc ctaaactgga
     1261 aggaaaagtt gcgtcaatga gtgagggtaa aaagaagacg gtaatcaaaa catggagcag
     1321 acgctctttg atatcaccag attttgttgg acatacattt gcagttcaca atggtaataa
     1381 gtttatccct gtatatgtaa cagaaaatat ggttgggcac aaattaggcg aatttgcacc
     1441 aacacgtact ttcagaggac atgcaggtaa taaagataag aaataatggg agcaagaaag
     1501 aaaatagcgg cagatgcccg caaagaagaa atgaaatcaa cctacattgc caggttgaat
     1561 aattgtccta cctctccaag aaaaatgaga ctggtagctg acatgatcag aggtaagaaa
     1621 gttgatcttg cattaggctt gcttaaattc agtaaaaaag aagctgcagg caacatgtat
     1681 aaactgttgt tatcagcaat caataactgg caggcaaaaa acgaaggtgt tcgcatcgaa
     1741 gatcagccat tagttgtatc taaagtttat gtggacagtg cccgtcagct taaaagatta
     1801 cgccctgcac cacaaggtag aggctacaga attcgtaagc gttcaaacca cgttacctta
     1861 ttagttgaca ctgtcaataa aaaagtagaa aacacaaaac aagtaactga aaatacagaa
     1921 gcataaatgg gacaaaaagc acacccgata ggaaatagac tgggaatcat ccgtggatgg
     1981 gactcaaact ggtatggtgg aaagaactat tctgaccgtt tagttgagga tgaaaaaatc
     2041 cgcaactatc tgcatgcacg tttggcaaaa ggtggagttg cccgtattat tattgagcgc
     2101 acactaaaac ttatcactgc aaccattcac actgcacgtc cgggtattgt tatcggtaaa
     2161 ggtggatcag aggtagataa gcttaaagaa gaaattaaga agattaccaa gaaagaagtg
     2221 cagataaaca tctttgaaat taaacgtcct gaacttgatg cacagttagt agctgatggt
     2281 gttgccaaac aaattgaagc acgtatttca tacagacgtg caattaagat ggcaattgct
     2341 tccagcatgc gtatgggagc agaaggaata aaaattatgt gtgccggccg tatcggtggt
     2401 gctgagatgg ctcgtacaga gcagtacaaa gatggccgta ttccattgca tactttccgt
     2461 gctgatattg attttgccat tgcagatgca caaacatctt atggaaaaat cggtgtaaaa
     2521 gtttggattt gtaaaggcga agtttttggt aagagagacc tttctccaaa tgttggaatg
     2581 aactcaacac caaagcagaa tgcaggcggt ggtgacagaa gacaggagag agaacgtggc
     2641 ggtaatgacc gtggcggcag aagaggtgga cgtccaagag gagagaagaa ataattattt
     2701 aaaaaagaat taaggttaat tagcaatgtt acaaccgaaa aagacaaaat tcagaaagca
     2761 gcagaagatg cgcaaccgtg gtaatgctac ccgcggatcg cagattgcct tcggatcatt
     2821 tggaattaaa tcactggaga atgctcaact tacttcacgt cagattgaag cagcccgtgt
     2881 tgcccttacc cgttttatga aacgtgaagg acaaacatgg atacgtattt tccctgacaa
     2941 gcctgttaca cgtaaacctg cagaggttcg tatgggtaaa ggtaaaggag caccggaata
     3001 ttggattgcc caagtaaaac caggaactat gctttttgaa gcagatggtg tgtctgtaga
     3061 aacagccaaa gaagcaatgc gtttggcagc acaaaaatta ccggtaataa ccaagtttgt
     3121 tattcgcaga gattattcag ctgcctaaca ttaaaatttg aaaaacgatg aaacagtcaa
     3181 tagttaaaga tttaacaaca gaagaagtaa aatcacgcat taaggaagaa gtggataatt
     3241 tacagaagca taaaatgcaa catgccgttt ctccaatgga taatccgatg aaaatccgca
     3301 gcactcgcaa acttattgca aggctgaaaa ctgagttaaa caaaagaatg tcacaaacat
     3361 caaaataaat tattcaatga gtactgaaag agcattacgc aaaacaaaaa taggtgtggt
     3421 taccagcatt aaaatgcaaa agactgttgt tgtggctgaa gaaaagaagt ttaaacatcc
     3481 tatgtacgga aagtttatta ataagactaa aaagtttcac gtacatgatg aaactaatgc
     3541 atgtggtagt ggcgaccgtg ttttaattat ggaaaccaga cctatcagca aatcgaagcg
     3601 ttggagatta gttgaaattc ttgaaaaagc aaaataatta aaatttctcg ccctactcaa
     3661 aggcagaatg tagaaaagaa ataaaagatg atacagtcag aatcaagatt aacagtagca
     3721 gacaacagcg gagcaaaaga agttttgtgt atccgtgtgt tgggcggtac acgcaaaaga
     3781 tatgcttcgt tgggagataa aattgtggta accgtaaaga atgctttacc tgcaggaaat
     3841 attaaaaaag gtgctgtaag caaagctgtg gttgttagag taagtaagga agtaagacgt
     3901 aatgatggtt cttatatccg tttcgatgat aatgcagtgg tgttactcaa tcaggcagat
     3961 gaactcagag gtacacgtat tttcggacct gtagctcgtg aactgcgcga aaaacagttt
     4021 atgaaaatcg tttcattggc acctgaagta atttaattta taaaatcaga atcatgtcaa
     4081 aactaaagat aaaaaaaggc gacatcgtta aggttattgc cggtgagtca aaaggcaaac
     4141 aaggccgtgt gcttgaagta tttcctgaaa caaacagggc aagtgttgag aatgtaaata
     4201 tcgtttcaaa acatacacgt ccgaatgcac aaaatacaca aggcggtatc gtaaagaaag
     4261 aagctccggt taatgtttca aatcttatgc ttattgatcc taagtcagga aagccaacac
     4321 gtgtaggtcg taaaacagaa gaaggtaaat tagtacgtta cgcaaaaaaa tcaggggagg
     4381 taataaaata atgagttaca caccgcgcct aaaaggcaag tataaaaaag aaatcatctc
     4441 tgcactgaaa aatcattttc agtataagag tataatgcag gtacctaaat tatcaaagat
     4501 ttgtcttagc cagggtattg gcgatgcagt tagtgataag aaaattgttg atgcttgtgt
     4561 agcagaaatg acagctattg caggacagaa agccgtagca acttatgcta agaaagatat
     4621 ttctaacttt aaagtaagaa aaggtgttcc tattggagta agagtgactt tgcgtgatga
     4681 gcagatgtat gaattccttg atcgtttggt gtcagtatcg ctgccacgta ttcgcgactt
     4741 cagaggaatc aataacaaag gatttgatgg taaaggaaat tacacacttg gcattaccga
     4801 gcatatcatt ttccctgaaa tcaatattga caaagtaagt aagattcgtg gaatggatat
     4861 cacattcgta actacagcta aaactgataa tgaagcaatg cagttactta aagaattcgg
     4921 actaccattc agaaccggtt ctaactaaaa aataattcga aaaaatggca aaagaatcaa
     4981 taaaagcaag aagcagaaaa agagaacatt tagttgaaaa gtatgcagct aaacgtgcag
     5041 cattaaaaga agctggcgat tatcaggcat tggcattgtt gcctcgtaat tcatcacctg
     5101 ttcgtttaca taaccgttgt atgattacag gtcgtccaag aggttatatg cgtcagtttg
     5161 gaatttcacg cgtactgttt cgcaaaatgg ctttagaagg aaaaattgcc ggagtaacaa
     5221 aagccagttg gtaatattaa acaataatta gaaaaataat taaaatgacc gatccgatat
     5281 cagattattt gacacgtttg cgtaatgccg taaaggccaa tcaccgcatc gtagaagtac
     5341 ctgcatcaaa tactaaaaaa gaaattacca aaatactttt tgacaaaggg tatattgcaa
     5401 attataagtt tgaagaaact gacaataagc agggaaatat taaagttgct ttaaaataca
     5461 atcctgtaac caaattgccg gcaattacgc gccttaagcg tgttagccgt ccaggtttga
     5521 gaaagtatgt tagtcatgat cgtttgccaa gaattatcaa tggtcttgga gtagcaatta
     5581 tctctacaag caaaggaatt atgactgaca aagaagcacg tcagcttcat gttggtggtg
     5641 aggttttatg ttatatttat taatttaaga aagcatgtca cgaataggga aattaccggt
     5701 tgcagtacca tcaggtgttc aggttaatgt acaaggaaat acagtttctg taaaaggtcc
     5761 aaaaggcacc ttatcacaaa ccatagccga tggcattact ttagaaatgg aagaaaataa
     5821 agttgttgta aaacgcagca cagagcaaaa acgtcataag tcattacatg gcttgtatcg
     5881 ttcattgctt gccaatatga ttaaaggagt atcgcaaggc tataaaatag agcaggagtt
     5941 agtgggtgta ggttaccgtg ccagcaatca gggtcagatg cttgatttgg ttcttggtca
     6001 ctcacaccat gtggtttttg aactgccgac agaagttaaa gtaagcacaa agacagagcg
     6061 tggtagcaat cctatgatca tacttgaaag tcacgataaa caacttatcg ggcaggttgc
     6121 tgctaaaata agatcactcc gtaagcctga accatacaaa ggaaaaggta tcaagtttgt
     6181 gggcgaagta ttaagaagaa aagctggaaa atcagctgct aaaaaataag taaagtaaga
     6241 aaatgaagaa ttcaaaagat tcacgcaggc ttaaaatccg taaacgtatt cgcggaaaaa
     6301 tcaaaggtac taatgagcag ccaagattgt ctgttttcag aagtaataag cagatttatg
     6361 cacagataat tgatgatgtt gcaggtcaga cattggctgc agcttcatca gcagaaaatg
     6421 gtgcagcaaa aggaactaag gttgagattt caacttcagt tggcaaaatg attgctgaaa
     6481 gagcaacagc caaaggtatt accaaagtaa cattcgacag aggtggatat ttgtttcatg
     6541 gccgtgtaaa agcattggcc gatggagcaa gagaaggtgg attaaaattc taaaacgtaa
     6601 caatgatagc aacagcaaat aaaagagtta aaccgggcga aatcgaatta aaagacaggc
     6661 tcgtagcagt aaacagagta actaaggtta ctaaaggtgg aagaacattt tcttttgcag
     6721 ccattgttgt ggttggtgat gagaaaggtg ttgtaggtta tggtttagga aaagcaaaag
     6781 aagttaccga tgcaattgcc aaagctattg acgatgcaaa gaaaaactta gtgaaggtta
     6841 cagttcataa aactactgtt cctcatgagc aagatgctgc tttcggtggt gcaaaagtat
     6901 ttttaaagcc agctgctccc ggtaccggtg taattgcagg tggtgccatg cgtgcagttc
     6961 ttgaaagtgt aggcatacat gacgtgttag cgaagtcaaa aggatcttct aatccgcata
     7021 acgtggtaaa agctaccatt actgctttag ctatgatgcg tgatgctgta gcagtggcta
     7081 atcagcgtgg tgtagattta aacaaagtat ttaacgggta atattatact catgggaaaa
     7141 gtaattatca aacaagtaaa aagcggaata gatcgcgata aaaatcagaa gctaacacta
     7201 aaagctttag gtttaagaaa agtatatcat tctgtagaaa aagaaaacac tgatcagata
     7261 aaaggaatga tcgaaaaggt tagacatctg gttgaggtaa cagaaatcaa ataagaaaag
     7321 cgttatgaat ttaagtaatt taaaacctgc caaaggttca acaaagaaaa gcaaaagaat
     7381 aggtcgtggt gaaggctctg gtcatggtgg aacatctaca cgtggtcata aaggagcaca
     7441 atcacgctca ggatattcac gcaaatttgg ttttgaaggt gggcagatgc cacttcagag
     7501 aagggttcct aaatcaggat ttaaaaactt cttcagagtt gaatacacag gcattaattt
     7561 agatgtgttg cagaagttaa acgaaaaatt tggtttaaca gaaattgatc atagcattct
     7621 ttgcgaaaaa ggattaatcg caaaacgtga gttagtaaaa atacttggac gtggcgagtt
     7681 aaaagtaaaa atgaatgtaa aagcacatgc attttcagag actgcaaaaa atgctattga
     7741 agccgctgga ggcacaacca ccattcaaga aaaataatga aagaactgat tcaaacatta
     7801 aggaacatat tcaagattga agagctaaga acaaagcttc tcaatacctt gttttttatt
     7861 gccatttacc gcttggcctc tcatattgtg ttgcctggtg tagatcctca gcaacttggt
     7921 aatttaaaga accaggcttc gcagggtata tttggcttat tagatatgtt tgccggaggt
     7981 gccttttcac aggcgtcaat tgttgcattg ggaattatgc cctatatctc agcctcaatc
     8041 gttgttcaga ttttaggaat tgctgttcct tacttccaga aaatgcagaa agaaggagag
     8101 agtggacgta atcgtttgaa tcaaataaca cgctggctta cagttttagt tgtggctggt
     8161 caggcaccag ggtatttagt aaatcttaga gcacaagcac aatctgctat tgttgcctat
     8221 tcaaaccctg atgcaatgtc accaacgttg tggtggatta cttctatcat cattcttatt
     8281 gcaggcactt tgtttgtgat gtggcttggt gaacgtattc aggaaaaagg aatcggaaat
     8341 ggtatttcat tacttatcat ggttggtatt attgcccgat taccatttgc cttacgcgat
     8401 gagtttttat ctcgactcaa tcagacaggt ggaggattaa ttatgttcct agtcgaatta
     8461 gtagcactgt ttgctgtggt cgtgataagt attatgttgg tacagggtac acgtaaaata
     8521 cctgtgcaat ttgctaagaa aataattggt aacagacaat atggtggggt acgtcagtac
     8581 atacctttaa aagtaaatgc tgccggtgta atgccaatca tttttgcgca ggcaattatg
     8641 tttattcctg caacagtagc acagttcttt cctgaatctt cttccatgca gggtattgca
     8701 cactcattaa cagatttcca ttctgtatgg tataatgtta ccttcgcaat actgattata
     8761 ttattcacct acttctacac tgccattaca gttaatccta atcagatggc tgatgacatg
     8821 aagaaaaatg gaggatttat tcccggtatt aaacccggaa aacaaacagc atcatttatt
     8881 gatgatgtta tgtcgaaaat tactttgccg ggctctatat ttcttgcgat agttgccatc
     8941 ttacctgcat tcgcaacaat attaggtacc agtaccaact ttgctcaatt ttatggtggt
     9001 acatcattgt tgattcttgt tggtgtaata ctagatacct tacaacagat agaaagtcat
     9061 ttattgatga gacattacga tggattaatg aaatcaggac gtattaaagg acgcacatcc
     9121 attaatgcac ctgtttaaaa tataaaattg tgcagattga atatcgctcc aaagaggaga
     9181 tagatttaat tagagttagt tctttacttg ttggaaaaac cattgcagaa gttgccaaac
     9241 acctgcgaga cggaataact accggagagt tagataagat tgcagaagat tttatccgct
     9301 ctcatgaagc tgtgcctgct tttaaaggct atcatggatt cagtggttcg ttatgtatat
     9361 cggttaatgc cgaagtggtt cacggaattc ccggagcaag agttttgcat cctggagata
     9421 tagtgtcact tgattgcggt gtagttaaaa acggatatta tggtgattcg gcttacacct
     9481 ttgctatcgg tgaagtagat cagcaggcag taaaactact gcaggttaca cgcgaatgcc
     9541 tttatcttgg aatagagaaa gcagttgccg gtaacagaat gggtgatgta agcagcaata
     9601 tccaaaaaca tgcagaggca aatggatttg gtgttgttcg cgaattggta ggacatggca
     9661 taggaaagaa acttcatgag aagcccgaag taccaaatta tggtaaggca gggcaaggat
     9721 tgttgttgaa accggggttg gtaatagcaa ttgaacctat gataaacatg ggaacaaaga
     9781 atgtgaagca actcaatgat ggatggacaa ttgtaacagc agatcaaaaa cctgctgcac
     9841 attttgaaca taccattgca gtggataatg taaaggcaga aatactgtct tcatttgaag
     9901 agatagaaag aagtgaaaaa gagaatttga atttatataa aatagattat aagcagcata
     9961 ttatagcata gtaagattag attaatggca aagcagacat caatagagca agacggaacg
    10021 attacagaag cgttgagcaa tgctatgttc agagtggaac tggaaaacgg acatgagata
    10081 attgcccaca tttcaggaaa gatgagaatg aactatatta aaattctgcc cggtgataaa
    10141 gtaaagattg aaatgtcacc gtatgatctg acaaaaggaa gaataactta tcgttataaa
    10201 taactgaagt aacgaatcag aataaaaatt aaggagatga aagttaaacc aagtttaaag
    10261 aaaagaagcg ttgattgtaa aatcgttaga cgcaacggta aattgtatgt aattaataaa
    10321 aagaacccac gctttaaaca gcgtcaggga taattacatc ttaaaaataa ttgtaacaaa
    10381 gaaaatagtt aatatcaaat ggctcgaata gcaggtatag atttaccaag aaacaaaaga
    10441 ggtgaaatag gccttactta tatttttggt ataggacgtt caacagcaca acagattctt
    10501 gatgagtgcg gaatttcgta cgataagaaa gtagatcagt ggaatgacga tgaactgaat
    10561 aaaatccgtt cattcattaa tgacaatctg aaaattgaag gtgctttacg ttcagaaaca
    10621 cagttgaaca ttaaacgtct gatggatatc ggtagctacc gtggtgttcg tcatcgttta
    10681 ggattgcctg tgcgcggaca aagaacaaaa aacaattgcc gcacacgtaa aggtaaacgt
    10741 aagacagttg ctaacaagaa aaaggtaact aaataatcag attatattga atcctgaaaa
    10801 tcagcattca gaataaataa aaagaataat ggctaaacaa caacaagcat cagctgcaaa
    10861 aacagcaaaa aagaaggtgg taaaagttga ggcgaccggt gaggcgcata tcaatgcaac
    10921 tttcaataac attattataa gccttacaaa tctgcaagga caggttatct cttggtcaag
    10981 tgcaggtaaa atgggtttta aaggttcaaa gaagaataca ccttatgctg cacagcttgc
    11041 tgcggctgac tgtgcaaaaa cagctttcga tttaggatta agaaaagtga aagtatttgt
    11101 aaaaggtccg ggagcaggtc gtgaatctgc aatccgctca ctcaatgctt ccggagtaga
    11161 agttagtgaa attcttgacc ttactccaat accacacaat ggatgtcgtc ctcctaaaag
    11221 aagaaggata taatttatta tcacaaatca gtttaaaaat taaaatatta gaataataca
    11281 atggcacgtt acattggacc aaaaacaaaa atagcacgta agtttcgcga gcctatattc
    11341 ggacctgaca gatatttcga aaaaagaagt tatcctcccg gaatgcatgg tgcagctaaa
    11401 aagcgcgcaa agcaaagtga atatgcaatt cagttagctg aaaagcaaaa ggtgaaatat
    11461 acttacggaa tgttggagcg tcagtttgct cgtttctaca gtattgcact tcgcaaagaa
    11521 ggcattacag gcgaaaactt attgaagctt attgaatcca gacttgataa tgttgttttc
    11581 cgtttaggaa ttgcaccaac acgtgctgct gcacgtcagt tagttggaca ttgccatatt
    11641 acagtaaatg gtaaggtgaa caatatttca tcatacatct tacgtccggg tgatgttgtt
    11701 ggcgttcgtg agcgttcaaa gtctttcgaa gcaataacag attctcttgc atctcgttct
    11761 aataagtacg gttggttaga gtgggacacc acagctatgc aaggtaaatt tgtgactaca
    11821 cctgcacgcg aagaaattcc tgagaatatt aaggagcaac ttatcgtgga attgtactct
    11881 aaataattct tgctaaccct gaacagtaaa actcagtaaa taattaaaca aataaaacac
    11941 aaaatataaa atggccatac ttgcatttca acgtcccgac aaagtgatta tgaatcattc
    12001 taccgaaaca ttcggtcagt ttgaattcag acctttggag ccgggttatg gtattactgt
    12061 tggtaattct ctacgtagaa tattgctttc ttcactcgaa ggacatgcaa tcaccaatat
    12121 taaaatcagt ggagttgatc atgaattttc aacaataaaa ggagttgttg aagatgtaac
    12181 tgaaattatc ctaaacctta agcaggttcg ttttaaaaag caaattgaag gtacagactc
    12241 tgaaaaagtt caggttgtag taaacggtaa aacagaattt actgccggtg atattgcaaa
    12301 acatacctct gcttttcagg tattaaatcc tgaattggtt atttgccaaa tggatccttc
    12361 tgtaaaattg gttattgata tgaccattga caaaggacgt ggatatgtac ctgcagaaga
    12421 aaataaatca aacagccatc cattaggaac tgttgcaatt gattctattt acactcctat
    12481 aaagaatgta aaatatgcag tagagaacta tcgtgtggaa caaaaaaccg attacgaaaa
    12541 attggtactt gaaatcacaa ccgatggaag tattcatcct aaagaagcat tacgtgaagc
    12601 agcagaaatt ctgattcatc acttcatgct tttcagcgat gagaaaataa cactcgatac
    12661 aaagtctaag actccggttg aagagtttga tgagaacaca cttcacatgc gtcaattgct
    12721 aaaatcgaaa cttgtggata tggatctttc tgtaagagca ttgaactgtc tgaaggcagc
    12781 cgatgttgaa actttgggtg atttggtttc gttttcgaaa acagacttgt tgaagttccg
    12841 taacttcgga aagaaatcac ttacagagtt agaagaattg gttcgcagca aaaaccttac
    12901 cttcggtatg aacatacaga agtatatgct tgataaagaa taattgaata gtttaaagat
    12961 tacgtaaaat gcgtcacgga aaaaagataa atcatttagg cagaacacat agccacagag
    13021 aagcattact ctctaactta gcttcatcat tgattctgca taagagaata aacacaactg
    13081 ttgccaaagc gaaggcactt agaaaatatg ttgaaccatt aattaccaag tcaaaatctg
    13141 acagcacaca ttcgcgcaga acagttttta gctatctgca gaataaagaa gcggtgagtg
    13201 agcttttcag agaagttgca ccaaaagtaa acgagcgtcc gggcggatat acccgtatcc
    13261 tgaaaacagg attcagaccc ggtgatgctg ctgaaacttg catgatggag ttagttgatt
    13321 tcaatgagtt gatgccttca tcaaaagaaa aaggtacagc taagaaagct aagactacac
    13381 gcagaggccg taagccaaaa gctaaggctg atgctgcagg aagcgagaca acggaagcaa
    13441 cggaagaata aattcaacag aaaaattaat ctaaagggga tgtcggtttc ggcatccctt
    13501 tttttatttt tatgtttaca gtgattttct ctgatagcac aaatatgttt gttcaaaata
    13561 cttctatacc ttttgctgat gcaggatatt ctgcattaat tactgattat ttaaacaaca
    13621 atattttttt aaaagaatat gcatcatttc aatatgatga atcaggtttt aaagaggcat
    13681 tacagaaacg agtaaagtat aaagccaacc gtgacaggct ttgcgaagta ttagagcagc
    13741 agtattcaga aacaattttt caaacagatt ggaagacgga taaatcatat tctgcaatta
    13801 aatctttgaa agaagagaat acttttacag tcactactgg tcaccaactg aacattttta
    13861 ccggtccact ttattttatt tataaaatac ttaccgtaat taagcaggca gaatttctgg
    13921 ctcagagaaa tcccggttat aaatttgtac ctgtttattg gatggccagt gaagatcacg
    13981 accttgcaga agtgaatcat atagatattg aaacatcgaa aatagaatgg caaacaaatc
    14041 aaaagggtgc tgttggcaga atgactactg aaggaatgta ttctgttgtt gaagcattga
    14101 aaaatcagtt aaataagaat gataattttc aggagttagc aacgatactt gatacagcat
    14161 atttaaaaaa tgcaacgcag gctaatgcta tcagaagcct tgtacattct ttgttttcaa
    14221 aatatggttt ggtgattata gatgcagatg ataaaagatt caagcaacaa gcaattaaat
    14281 tgtttgaaga cgatttgttc aataacacat catttaattg ttttaaaaat ctttctgatt
    14341 ttgaaaaagc gtatggatta caaatccatg caagagaaat taatttgttt tatttagtca
    14401 atggagaccg tagcagaata attaaaaatg gcgaaagatt tgaagttgca gattcaggtt
    14461 tatctttctc aagtgatgaa ttgaaaaagc atatcaatga gtatcctgaa cgatttagcc
    14521 caaatgttgt actacggcca ttatatcagg aaataatttt accaaacatt gcatatactg
    14581 gcggccctgc agaagtacat tactggttgc aattaaaatc tattttcgat aagctgaaag
    14641 ttttttatcc catggttgtg cttcgtaatg gtatgatggt agtgaatgca gatcaagcta
    14701 ctgttttgaa tgaatcggct ttggaatgga aggatttgtt tcatccaaca gaacaagttg
    14761 tgaatattgc attgaaaagc aagtatggtg aagaactaag tctaaaggag gaaaaggata
    14821 ccttgcaaag gagctatgaa gaaatggagc aacgttttaa agcaattgat ataacacttg
    14881 agaaacatgt taaggcagag tggaggcgaa cagaaaaacg gttgatggct tcagagaaaa
    14941 aacttttacg tgcatttaag aaaaagcagg ttgatgaaac ttgtaaaatc agaaatacag
    15001 tgaaacaatt gttcccaaat ggtaaattgc aagaaagaaa agaaaatgtg tttccttatt
    15061 taagcaagta tggattgcag tttattgatg atttgtataa aatttgtgaa ccactaccaa
    15121 caaatttcaa aattgcggta acagcagaaa aataaaaaaa cttaaagtaa ttcgtaaata
    15181 cctgcagcac cctgacctgt accaacgcac atggtgacca tgccatactt catctttctt
    15241 cttttcattt cattaagaag ttgaacacta agtttggctc ctgaacatcc taagggatgt
    15301 cctaatgcaa ttgcaccacc atttacattg gtaatttccg gatttaatcc taaggtcctt
    15361 ataacagcca gcgattgtga tgcaaaagcc tcattgagtt ctattaattc catatcattt
    15421 tgcttgagtc ctgcgcgttt cagaactgat ggaatagcat aaactggtcc tacgcccata
    15481 atacgcggtt caatggcgct gacagcataa gaaacaaaac gtgcaattgg ttttacgtta
    15541 agcgttttga gcattttttc agacactaca agtacaaatg atgcaccgtc actggtttga
    15601 gatgagttac cggccgtaac agttccttta gcatcaaaga caggctttaa tttatttaat
    15661 ttttctattg tagtatcctg acgtggtcct tcatcagtat caacagtact tttatgtgtc
    15721 tgccgttttt cattggcatc aagataggtc tcattgattt caacgggaac aatctgctct
    15781 ttaaacaaat tattttttat ggcattaatt gctctttggt gtgattgaaa ggcaaactga
    15841 tcctgatctt cacgattaat tttaaattct tttgcaactg cttctgcagt taaccccatg
    15901 ttccaatacc attcaggatt atttgccgcc acctcataat taggaacaat cctccatccg
    15961 ccaaacggaa taggcgacat acattcaaca ccaccggcaa taatacaatc agctaggccg
    16021 ctatgaattt ttgcagaagc aatagcaatt gattcgagcc ctgatgaaca atagcggtta
    16081 atggtcatac caggaacttt aactgtatta agtcccatca aagaaatcat tcttccgata
    16141 ttgaggcctt gttctgcttc gggtgtggca ttacctgcaa ttacatcatc aattaattct
    16201 gtatcaaaat tcggaactga ttgtatgaga tgttttataa cagttactgc aagatcatca
    16261 ggtcttgtat ttttaaattt tcctcttggt gctttgccaa cagcagttct atatccggct
    16321 acgatgtatg catccatttg ttcttgctat ttaataaatt agtgttggta gcaggttcta
    16381 attacaaaag aaatttattt cttttccggt gttgaaggtg gtggtgaggg tgtttttaat
    16441 ttagcccctg gaggtaattt ctttttatca tcagctgctt ttttagctgc tgcagccttt
    16501 ttagcctttt catcagcttc cttagcagct gccagtgctt ttgcatcctc acccatctta
    16561 tattcaacct gactgattaa tttacctttt ctgtcataaa ccttccacaa accatttttg
    16621 agatttactt tatatggacc tgtatttcgt atttcaccat cactgaaata tgttgtccat
    16681 gttccacttt ttttaccatt taaatattca ccttccatct tagcttgccc attgtaataa
    16741 tatcttttgt attctccatc ttttattcca ttggcatact ctatctcctc cattatcttt
    16801 ccatagggat aatatttttt tgctatccca tttctcacat catctaaata ttcaatttcc
    16861 tgatttaatt caccggcttc attataatat ttccaaacac cttgccgctg tcctaattca
    16921 tctgttttgt tgcgatgttt ttctcttccg ggcgcatatc ttggtttttc ttcgcgttga
    16981 gcaaaagcaa aagccggtaa aaggattaac aacaacaaca gttgttttaa tattttcatc
    17041 ttttatgatt ggtatggtaa ttcaaatggc taaaataaaa tatttttaat caaaacatga
    17101 atgaaattca gagtttttct acatgttttt catttataaa cagctcattt aatttctgtt
    17161 tttcagagga aaaacaaata atggccttag gtaaattgtt gaaaacccat gaattttaaa
    17221 atgctgacac aaatgattaa atattaattc cttaacgctt tacctgtagt taaaattgcc
    17281 tgaatgcgct caagtgtttt cttttcgcct aataaagaaa gaaaagcttc tctttccaag
    17341 tcaagcagat atttttcaga tactaaagat ggcgaactta aattgccacc acacataatg
    17401 tatccaagct ttaacgaaag catttgatcg tattggctga tgtagttgcc tgccagcata
    17461 gcacttgcac cggcctgtat aacgccaagc ccttgtcttc ctaaaacacg aatatcatgt
    17521 cttggttgcg ggctcacata tccggcatta gctaaagata aaactgcatt tttagcatca
    17581 gataaaagtc tttttcggtt aagcgtaatg ttgtcaaaac cttttctaag aatatgcaag
    17641 tcgtaagctt caaatgccga agttgaaact tttgcagtgg caatggtcat aaaaaaatcg
    17701 cgtaatgcat ttgtttcaat atctccgggt ttaatggagt ccgaaaaacg aagtgtcaat
    17761 tctttggtac caccacctcc gggaatcagt ccgacaccta actctacaag accaatgtaa
    17821 gtttctgctg ctgcttgcac tttgtctgca tgcaaactca tttcgcaacc acctcctaag
    17881 gtaaggccgg caggtgcaac tacaacaggt acattgctat agcgtaaacg catattggta
    17941 ttttgaaatg ctcttacagc ataatcaagt tcatcatatt cctgttctac ggcaagcata
    18001 aaaaccaaac ccagatttgc acctgcagaa aaatttgctg cttcgtttcc aattactaat
    18061 cctttaaaat ctttttctgc aaaatcaatt gcagtattta ttccttgtaa tacttcacta
    18121 cctattgtat tcattttggt tttgaattcc agattcaaaa caccatcacc aatatcgtgt
    18181 aacaggcagc cggaattttt ccagacaatc ttttcgtttg aagtgttttt aagaattact
    18241 aaactgcctt cgcccggaag ttgatttaat tttttagtct gtaaatcgaa atatgatttt
    18301 ttgccattat caattttgta gaaagaattt attccattac taatcatttc tgtcacccat
    18361 ggtgaaatgc tataaccttc tttttgcatt tgttctacta cggattttac tccaagaata
    18421 tcccatgttt cgaaggggcc gatttcccaa ccgaaacctg cacacatggc atcatcaatc
    18481 ttgtaaattt catctgcaat ttcaggaatg cgatgcgaaa cgtaggagaa taaggagaaa
    18541 aacatgtaac gataaaatga tgctgcttca tcagtcccgg agagcaacat aggaagtctt
    18601 tgtttcagat catcaacagt ttttgtactt tctaatgttt taaattttat tttagctgat
    18661 ggcttatatt ctaaagttgt taaatccaat tcaagaattt ctgttttacc ttctgcagtt
    18721 tttgtttttt tataaaagcc ttgtttggtt ttatcaccca gccatttatt ctcaatcatt
    18781 ttattgataa atggaggaat tgcaaataaa tttttctgtt catcatcagg caaattttta
    18841 taagcatcat ttgccacatg taccaatgtg tctaatccaa ctacatcact tgttctaaag
    18901 gttgcagact ttggtcttcc aattatagga cctgtaagtt tatcaatggc aaaaactgaa
    18961 agacctgttt tttccattga atgaaacaat gacatgatac caaatacacc aattctgtta
    19021 gcaataaatg caggagtgtc tttgcataga acagttgttt tgcctaagta gcgatcaccg
    19081 aagtgggaaa taaaatcaat cacatcctta tcggtaaatt gagtgggaat gatttcaagt
    19141 agaggaaggt aacgcggagg gttaaagaag tgtgtgccgc agaaatgttt tttaaaatca
    19201 tcagagcgac cttctgtcat caaatgaata ggtattccgg acgtgtttga tgtgattagt
    19261 gttccgggct ttctgaattt ttctacttgt tcgaataaag ttcgtttaat atttagatct
    19321 tcaataatta cttctataat ccaatcgaag ttagcaatgt cttttagatt atctgtaaaa
    19381 ttccctattg aaattctttg tgcaaagctt ttatgataga gtggagaagg gttacttttt
    19441 attgctgact gtaaatgagt atctgtaatt ttatttcgaa acttcggact atcttcaaca
    19501 aaacctgctt ttttatcagc ttctgtaaat tcttttggtg caatatctaa tagcagtaca
    19561 ttaaaacctg catttgcaaa atgacatgca attcgtgaac ccataattcc tgaacccaga
    19621 acagctactt tttcaatttt tcttctcata aaaactatct aaaaaataaa acccaaacct
    19681 aagttatttt acgaataagg tttgtaaaaa ccagataaag ttatgaaaaa caacagaact
    19741 gttttgtcaa ttagtaacta attgtaaata gtttctttag gatgtctgta tgagagtaag
    19801 ttgcaattac ctgcattcag ttcattccag gcatcaatct tagattcaat cattgctaga
    19861 ccactggttg aaaaatgcaa actctctgta ccagttaaaa atttcaccaa ccgctctaaa
    19921 cccggattgt gagcaattag agctacagag ttaatttggt catctaagct ttttaatatg
    19981 gcaagcatat cttttgaacc tgtcatatat aggttctttt caagttgaat attgttagga
    20041 tgatagccaa gatgtttgct atagattaaa gcagatgcat aagctctgac tgctgtactt
    20101 gtcaaaatta aatcaacgga gtttatgtga tcttttaaaa tcaaactcat cttttcagta
    20161 tcgtgacaac ctttttctgt tagcggtcta tcgctgtcat taatttgaga atactcgcga
    20221 gttgctttgc tgtggcgaat tagaaaaatt gttttgggca tagttggtag ttaaattttt
    20281 aaatagggtg attaaaaaaa attaaattag ttaaatcatg caacaatttt aacaaaaatg
    20341 atgccttttt gcaagttatt atttaatttt ttttgaacgg gtataaaaca agttaagaaa
    20401 atgacactat tttgtttctt tgcttgtgca tgaaacagga aatgttgaag tttttatcaa
    20461 tagcgcaaag caaaaagcgt gaaaacaaac aggttttgaa agacctttcc aagtctaaat
    20521 cgcctcagtt agataattta ttcagagagg aaagtgataa tttcttcaag aaattcagtt
    20581 gcctggattg tgccaattgt tgtaaaacaa caagcccaat tttcagagat gttgatattg
    20641 aaagaatcgc agcacattta aaattaaaac cgggagattt tgtagcaaaa tacctacatc
    20701 ttgattctga tagccattat gttcttaatc attcaccatg tcctttttta ggaacagata
    20761 attattgttc aatttatacg gtacgtccgc aggcgtgtag ggagtatccg catactgacc
    20821 gtaaaaatat caatcagata atggatttaa cttatcagaa caccttagtg tgtcctgcag
    20881 tggcgtcaat agttgaaaaa ttaaagacgg attttattaa gaaatagaaa tgtaatgatt
    20941 cttatggatt gatgagtaat tcatctaaat ccattatcaa ctccaactca tgaaaatagt
    21001 cggcaatttg tccaagcatt tctttttctt tatcagacaa ttcaactacc cttgattttt
    21061 ttacttcttg tttgccactg ttgtttttaa atgctaaggc agtaagttct tcatctgaca
    21121 aaacatattg aagtaaactc ttaaagaaat tttttcttga agcaggatcg agtttttcaa
    21181 ccatttgctg caagagttta atacgctctg cttttcttaa gttaagataa tggtcaaagt
    21241 cgaaattttt tactttagaa aggttattag attttttttg tttctgcttc attgctatga
    21301 atttttatat ttcatacgat gaatatgcaa gcaggttgca aaaagaaaaa caaaaatctt
    21361 acaaaactaa aggtgaaatc tgctgattgt tttttcctgt aattaaggat gcatcaatct
    21421 ggttttttgg tacagtaaag aaaaaagctg aaccttctcc gggagtggat tcaatacgaa
    21481 tgctgccacc agcctcttct acaatttttt taacaataga aaggccaata ccggtagatt
    21541 caaattcatc tcttgctttt aatgtctgaa atattacaaa cactttttca tgatatcgtt
    21601 tgtcaattcc cgggccattg tctttaatct taaactccca aaacttacct tcttcatgac
    21661 aactgatttc aatatgcttt tgctctttgt tattgtgttt aataccatta ctgataaggt
    21721 tcatgaatac ttgctggtat ttaactttat cgccataata ttcaggcatt gcacctgaaa
    21781 tttctatttt acagttactg tcagcaccgg ctaaatcaac agcattatgt acaatttcat
    21841 taaatgaaaa tagctcttga gtgcctttat gttttgaagc ttttgagtat tccaaaatgg
    21901 cattgataag ttgttccatt cgttttaccc ggcctttaat aatctggaaa ttttctttcg
    21961 attcatctga gaggctgtct gcactgtctt gggtaatcca gtcagtcagg ttaccgatgg
    22021 cacgtaatgg tgctttcaga tcatgcgaaa caatatatgc aaacttgtca agttctttat
    22081 ttgttttttc aaggtcactt aagtattcgc ttaactgtat ttctgctttc ttacgttcgt
    22141 taatatcact catgataaaa atgaacttgc gagaattttt ttcattgtcg cttattggtg
    22201 taatactggc atgcatccac actgttgttc catctttttt gtaacgcaaa agttcactgt
    22261 cacagaattt aaattctcgc atactgttct gcactgcctc aagattttcc ttgtcggttt
    22321 cagggccgct taagaaggtt tgcagtgatt gatctattgc atcataaaat tgataacctg
    22381 ttattcgtgt aaaaccatca ttgacccatt caatgacacc attttcatta gtaataacaa
    22441 cggcattgtc tgtcttgctg gctactaatg aaagtttttt aagttcttta ttgatgatag
    22501 caagtttgtt tcttgaatct gtgatggtag aaatgactac atttttttta ctctctaaat
    22561 tgttgatcat gctgctgatt acaatcaaac tgacaagccc tgtaatcaaa taatcctgat
    22621 tgataagaga tgcagtttcg ccaatgaaac tatagtcaat ccagttagtg tattcagtta
    22681 tcaaaaagaa ataaatcatg ttggcaacag acagtattgt gaaccaaatg ccataacgac
    22741 tgccaagtag cataaatgaa atgaggataa aagcaatctg ataaaacatt cctgagttac
    22801 gaatgccacc ggcaaagtag gttatgtaat gtaaagcacc aaagcaagat aatagactaa
    22861 tgactactga tagtgtgagg tttttatgta cattaagcag taaataattc aaaataagca
    22921 gtgactctaa gcctaataga atataggcaa gattcttttc ggtttgaaaa acataaattg
    22981 cctgataaca gattaaacct atacctatga tagcaacaag gttaaagatt ctgaaaaggt
    23041 tgaaacgctg cttagctaat atgtcatcat cctgaataga acgataaata ctatcgagca
    23101 ttaattttct gataatccat tttacaacag gtgtatccat accggtttgt gtagaaaatt
    23161 gtgttaaact taaggtgttt gtacgttact gacaaataaa atgttttatt tttgcagata
    23221 aatatttagg tgatatattt ggcaattaat gaataagcaa ttaacgatac ttcttattga
    23281 tgatgataag gttgacagca tgataatcaa gagggcaatc gctaaacttg attttccata
    23341 tcaggttgat acagcccaca atggtcttga tgctctaatg atgctgcgcg ggatgagtga
    23401 tattaggatt aaaagacctg acattatttt gttggacatt aatatgccgt taatgaatgg
    23461 acttgaattt ttatctgaat tacgtgccga tccggagcta aaagatattc atgtatgtgt
    23521 tttaaccact tctgaagatg attctgacgt aaagactgcg tatgagttta atgttgccgg
    23581 ttactttatt aagccattga atattagtga ctttgatgat cgttttttat atttaaatgg
    23641 atattggcaa cgtaattgct ttccggttca ataagtctct ctttcttttt aaagacattt
    23701 gttttagtga ccttgtaaat tggttgaaaa tctttcgact ttcgacctga aatatcttgc
    23761 attaaccaaa ttcttattgt tgaaaacttt agccgatata ttttggcggg ctaatctcaa
    23821 cgatggactc ccaaacccgt tatataccac atctggatgg aatacgtttt attgcagcta
    23881 tgatggtggt ttttaaccat tatctatccg aactaaattt tgattataaa ttctctaccg
    23941 gtttttatgg tgtgcagata ttctttgcta tctccggatt tctgataaca ggaattttac
    24001 taagactgaa aaatgctgag aaagtaattc caaggcgact tattatcaaa aacttttttg
    24061 ctaaacgttt cctcaggctt tttcctccgt attatctttt aatattcagt tatatttttc
    24121 ttgagtgttt tggattgtac attgcacaga aaggttggat tgtattttat ttagcgtatt
    24181 tacccaactg gtttttttat tttcatggct ggcagggaac cattgataat cacctttggt
    24241 ctttaggggt agaagagcaa ttttatttgt tatggccatt tgtattattg ctgcttccaa
    24301 agggtactga gtttaaatgg ataatcacca tgattattac cggttatctt tttaaatgtg
    24361 tcatcagaca atattttatg ttaggaggag atcctttttt attgcccatt tcacaaatgg
    24421 atactctggg tattggagca ttgctggcat gtggttttga atatcgaatg cagtttatag
    24481 aatatttcaa aaaagcatta ccttatttgc ttcctttagc aatattatta accctggtta
    24541 catcttatga taagccacgt aatgatccat ttttatgttt aagcctgata ttactttcaa
    24601 gtagccttgt attaaatgct gtggtaggat ttaagggcgt tgtaaaaata tttatggaga
    24661 acaaatgggt ggtttacgga ggtaaaatca gctatggaat ttatttatac cacaaggtta
    24721 ttcctacatt gtttatcagt tcagttgaaa gagtaggaat tggaatgcca aacaggtggc
    24781 tgacattggt agtgtcaata gttttgactt ttttaatttc gcacttgtcg tggaagttaa
    24841 ttgagtcgcc tatattaaag ctaaagaaaa actttgagca ttaatgattg gcaaactctt
    24901 ctttaatttg ctcgaaactg gtgttgtttt ctttaaacac tacaggtaag ttttccgcaa
    24961 gggtctctaa cagttcatca atatcttcta tctgaatatg attacttaat gtaatacgta
    25021 atcctgtgtg tttaatacta acagcaggga ataccgatag gttgaagaaa aagcctgctt
    25081 ttttcattct taaaacagta ctgtatccaa cattaggatt tccaactcca ataaaaaata
    25141 caggagatct ttcttttctg atgaccaata aacctaattc ttcggctttt ttattgaaat
    25201 agtcaatttt tgccatcagg ctttgttgca aaggaataat ctcatcactc atgtgtattt
    25261 tacacgaagc aatagtagca gcaagaattg caggaggcaa ttgaattgaa aaaataaatc
    25321 ctttaccaca tgttttaact aatttaaaat actcttcatc aggataggta agtagtccac
    25381 cggcagtacc aaatgcctta gtcatgcctg tggaaagaaa cagtttgcga tgataatagg
    25441 gaactgcatt cataaccata cctgtaccgt gtggacctgt ccaactcatt ccatgaatat
    25501 cgtcaagata aaggtgcaat tgttcgtact tatctgcaag tgcataaagc tctttaaccg
    25561 gtgcaaaatc accatgcatg gaataaactc cgtcacccaa ataccaaatt ttctgatatc
    25621 tgtttttcag aacttttatt ctatcttcaa gaatatccat tctgttatgg cggacaatgt
    25681 ctattttaac attacgcaat ttaagcattg ctgtagcaga ttgaatgcta tcatgtgcct
    25741 gaacatccat gattattgca tcattatcac caacaagaag gggtaggcct gttatatgac
    25801 caagtgtaag cgattgcatt accaatgcag gacggccaaa catttttgaa aacagacttt
    25861 cagcttcttc gtagtagtga gatgatacat aagcacgcga agaagaaaat tgtgcgccat
    25921 gcgagtgaag tgcttctact gctgcttcaa taattttagg atgcttttca atgcccagat
    25981 aaccacaagt gccaaaaaac aatttctttt gaccttctaa agtaatatag cggccatcca
    26041 tttgttcatc ttgtgcttta caatgcacaa taccggcacc aatggtgatt tttgcaacac
    26101 cggtaataac gtctattgcc tgttgaaact tacttttctg ctcttgattt tccatcccta
    26161 acccaaaaaa tgattcgcgt gcaaaaatag cgtttgtaat agcaaaatac tgatgatatt
    26221 tatcaaattc agcttaaatt ctgaacattt catcgttcaa agccaatttt cttaaaagtg
    26281 ggcatactct ataacggtct tcgccatagt aggcaaaaag ttcttccatt ttattataaa
    26341 ccgctcgcaa cccacgttta tttgcccaag taagcagccc ttgagggtaa ttaactcctt
    26401 tagtcatggc agtttcaatg tccacagcag ttgcaattcc catcatcaat gcatcagcag
    26461 cttcgttaac caacatggca aaaatgcgat caaaaatctg gctatgtagg ttgttgtcta
    26521 aagatggttt aggctgaatg ttatttttat aatcataata ccctctgttg cttttacgtc
    26581 catagagttt cccttcatat aatcgtttct gtgtaataga aggtttatat cgtgggtcga
    26641 aaaacatctg tgtaaatacc gtttcagtaa ctttaaaatt gacatcgttt ccaataaaat
    26701 ccatcaattc gaatggcccc attttaaaac caccaatagt tttcattgca tgatcaattg
    26761 tagcaaagtc ggcaatacct tcctcataaa tccgaagcgc ttcgccataa taggggcggg
    26821 ctatgcggtt aacgataaaa ccgggtgtat cttttgctat cacaggtgtt ttactccatt
    26881 gtctgataat ttcaaccgaa ttggttaata ctttttcgga ggtgttaaat gacggaatta
    26941 cttcaaccaa ttgcatcaaa acaggtgggt tgaaaaaatg taagcctatt attctgtctt
    27001 gttgattgca tgtagagcca atagcaatga caggcagtga tgatgtgtta gtagctaaaa
    27061 tacatttgtc agaaacaatg ctttcaacac ttttgaatag gctctgtttg atttcaatat
    27121 tttctataac agcttcaatg aaaaggtcag aacttttcaa ttcatttaaa ttatcagtaa
    27181 atgttatctg agagaaaata gaatccgata atgtcttttc aattttgttt ttttcaacca
    27241 atttatccaa actgcttcgg atagatgctt ttgctttttg taaagcttca atgttagtgt
    27301 caaaaagtgt aactatattg ccatttgtag cagcaacttg tgcaatgccc gaccccatag
    27361 agcctgctcc tgcaattcct acctgttgtt tcattgtcct ttaaaaacgg gttttctttt
    27421 ttcgaggaat gcttttatac cctcgctgtg atcataactt tttgctgcat tgacttgttc
    27481 ttccaactca cattcaagtt gtgtattcaa gttattgcta agcgattgat ttagtaattt
    27541 ttttgtaagt cctaatgctt tggtaggtaa attggctaat gtttgtgata tctgcatggc
    27601 agtttccatt aacaatgcat catctactga tttatatatc atacccattg aaacagcttc
    27661 tgctgctgaa actttatcac ccaacatagt taatgcactt gctctttgaa aaccaataag
    27721 acggggcaag aagaatgtcc cgccactatc aggaatgagt ccgattttac tgaatgcctg
    27781 aataaatact gctgactctg ccgctaatac aatatcacaa cacaaagcaa tatttgcacc
    27841 tgctcctgca gcaacaccat taacggcaca tactataggc ttttctatat ttctgattag
    27901 agtaataata ggattgtaat gctcacgtac tattgcggca atgcccggac cgttggtgtc
    27961 aacagcttct gataaatctt gacctgcaca aaaagccttg cctgttcctg taatcaaaat
    28021 tgctcggatg tctgaattgt ttgcagcttt ttttagttcg tcctgcaatt gcaaagacat
    28081 tactcggtta aaactgttga atttttccgg acgatttaag gttaattgaa caacctgtcc
    28141 ttttatttcc tgtaatactg ttgattccat aattggcaaa aataaatagt tatcggaata
    28201 atcagtaaac aataattgat gattcactat tgcagaataa agttttttgt ttactttaga
    28261 gttctctttt agcagaattg atatatggag agcaattttg ccacaccatc aaatttagtt
    28321 tcagattctg tatctgcgtt ttggcaggta catagatcta aagagtctgc attacatgaa
    28381 actatcatcc ccaaaggtgt aatagaaatc attttcaatt ttgaaaccac acagctacag
    28441 gcacaggtaa ataatagaca tataacagta ccaagatgtt ttatacaagg atatagtact
    28501 tcgcctatac atcttcacct tgatggaagt caaacttttt ttggtgttgt tttgaatccg
    28561 gcagcagtta agtacatttt taatttctat ccgcaaatat ttgcaaactg tgttattgac
    28621 ttgacactaa ttgatagtac attttgtttt ttatggcata aacttggcga acaaaacaat
    28681 tttaataacc gggtgcaagt ttttacagat tggttaataa acagacttcc ggagctcaac
    28741 gaaagagaaa aggcattcaa ttgcttttta aattcagact ctaacattca attatctgta
    28801 acacaattcg caagcaacct ggagaaccaa acatgcagat ttttatttag gttgcgaga
//
LOCUS       DAOOOL010000013        28530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_128_length_28530_cov_206.335417, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000013 DAOOOL010000000
VERSION     DAOOOL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28530
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128_length_28530_cov_206.335417"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKU82_02270"
     CDS             <1..613
                     /locus_tag="PKU82_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81729.1"
                     /translation="DYIIRFQNTGNDTAFNVKVLNPLDTNKLQLNTLEYVASSHPVNM
                     NFIYHERNMEFKFDNILLPDSNVNEPLSHGFIHYRIKPKTSLILTDTIKNTAAIYFDF
                     NDPVITNTATTHVVLPTGIASYQSAENIQLFPNPAQSELNIQQIAAVEKIQVIDVLGK
                     IVFEKKIKSQQNLKINISQFQNGVYFVKAYSAHDVVVKKFVKR"
     gene            852..3506
                     /locus_tag="PKU82_02275"
     CDS             852..3506
                     /locus_tag="PKU82_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81730.1"
                     /translation="MRKLIILLLICTTTAHAQEIEWQNTIGGSGVEFLNYTQQTSDGG
                     YILGGRSNSGVSGDKTEPSKGDYDYWVVKLDATGNIQWQNTIGGDDADWLQSVQQTTD
                     NGYILGGWSKSNISADKTENSQGASDFWVVKLDSIGNIQWQNTIGGDSTDNLYSLQQT
                     TDGGYILGGFSISSISGDKTEYSIGGSDYWVVKLDASGNIQWQNTIGGYENDNLYSLQ
                     QTTDGGYILGGRSMSNATGDKTENSQGLDDYWVVKIDATGNIQWQNTIGGNSWDELES
                     IEQTSDGGYILGGSSDSDNSGDKTENSQGDFDYWVVKLDATGNLQWQNTIGGDNWDKL
                     KSIQQTTDGSYIFGGYSSSNISGDKTENSQGNYDYWVINIDSIGNILWQNTIGGLYAE
                     ALSSIKQTIDGGYIIGGQSVSPISGDKTEDSQGNNDYWVIKLTDKYNLIDGKMYADVN
                     NNTIQDVGELGVSNKMVTEQNTGRLSFSQQNGKYSLSVLDTGGFSVSASTINYYNSVP
                     LVHTAYFNNINQTDSLNDFALQPNGVINDVCINLTPVSLFRSGFDATYYISYINNGTT
                     TLTPTVIFFPDANVSFTSANVTPTSVTTDSVVWNIGALAPFQSGSIVVTVNVHTGLPI
                     GSLINSSVRIEPVAGDANPGCNYSSWEVFTTGSFDPNDILVSRDTLFTTEIPNPPYLD
                     YIIRFQNTGNDTAFNVKILNPLDTNKLQLNTLEYVASSHPVNMNFIYHERNMEFKFDN
                     ILLPDSNVNEPLSHGFIHYRIKPKTSLVLNDSIQNNAAIYFDFNDPVITNTATTHVVL
                     PTGIASYQSAENIQLFPNPAQSELNIQQIAAVEKIQVIDVLGKIVFEKKTNSQQYLKI
                     NISQFQNGVYFVKAYSTQGVVVKKFVKR"
     gene            complement(3612..4541)
                     /gene="queG"
                     /locus_tag="PKU82_02280"
     CDS             complement(3612..4541)
                     /gene="queG"
                     /locus_tag="PKU82_02280"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOZ81731.1"
                     /translation="MNRSQTTAWVKAEALKLGFSHCGISKAGFLEEEAPRLEQWLTAN
                     MHGTMSYMERNFDKRLDPTKLVEGARSVISLLFNYYPEKKQNPQAPQISKYAYGTDYH
                     FVIKEKLFQLLELMRQHIGAIDGRVFVDSAPVLDRAWARKSGLGWIGKNNNLINPQSG
                     SWYFIAELILDIELDADAPIKDYCGTCTKCIDACPTEAIVMPHVVDGSKCISYLTIEL
                     KNEIPVSFQNQFKNWMFGCDICQDVCPWNRFAKPHQHQELQPKSELIEMTKHEWTELT
                     EEIFKKIFKDSALQRTGYKGIKRNLNFLKSEED"
     gene            complement(4548..5582)
                     /gene="ruvB"
                     /locus_tag="PKU82_02285"
     CDS             complement(4548..5582)
                     /gene="ruvB"
                     /locus_tag="PKU82_02285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOZ81732.1"
                     /translation="MNKNLDPASENLSTAEKEIERALRPLSFDDFAGQRKVTENLTVF
                     VKAAVQRGEALDHVLLHGPPGLGKTTLANIIANELNVKIKVTSGPVLDKPGDLAGLLT
                     NLQANDVLFIDEIHRLSPIVEEYLYSAMEDYKIDIMIESGPHARSVQLKINPFTLIGA
                     TTRSGLLTSPLRARFGINSRLEYYDARLLQDIVMRSASILKTAIDEEAAFEIARRSRG
                     TPRIANALLRRIRDFAQIKGNGTVNIEIARYGLQALNVDIDGLDEMDNRILNAIIEKF
                     KGGPVGLNTIATAVGEEAGTIEEVYEPFLIMEGFLMRTPRGREATEKAFKHLGKKSLK
                     QKGTLFDSES"
     gene            5816..7837
                     /locus_tag="PKU82_02290"
     CDS             5816..7837
                     /locus_tag="PKU82_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ81733.1"
                     /translation="MKRLIAVHIFMLLCSAGQASHIMGGDFTYTLIGSVPQQQKKVYK
                     ISLNYYRNCDTSAAQFQPYYDLYICYDSSNANYKRAYAQISLDKISTQPIVPPILDTT
                     CYYNNNWCVEKTYYEATVTLSEDTTWHLFLSDGARNVSINNLIQPDSTGIFFYTVIPA
                     GISNSTPQFSNSPTPFICNLDTMQLSNLAFDSDGDSLVYKIVQPYGYIGLNNLMTYQD
                     INFNLYYLFPLPTVIYGPGYSYTSPFGAGGLCSINSQTGLLTISSPNQGLYVLAIEVE
                     EYRNGTLLSRTRRDMQVVVAACSYNTAPQLTSPVVLNYQIDEGDSICFPLTFTDAENN
                     MINISATGNLFGTALTNPPAQFVVNPTPNNNTAQFCWKTICGQAAPFPYMFTVESTDN
                     GCPSKTNTYVFSIRVKTIPHNQLPSINLSMYPDTPTCTGTAFQFNASTQFAGNNPTYQ
                     WYINGQALSNNYPNYTTNQLNNGDSVSVQLISNSICVIDSSAISNTQVATVLQPTVAS
                     FITSTVSVSSINVLQQFTNQSVGNTYNFWNFGDSTQSAEINPQHVYENSGVYLVQLVT
                     VSDDGCRDTTEAPVDIKTTMSFFMPSSFTPDDNGLNDLFTPIGAEMPDYTLQIFNRWG
                     QLLYSETGKPQWNGRYKNQLLPLGMYIYSIRMHNPTDPIIKTGTVMLLR"
     gene            complement(7968..8747)
                     /locus_tag="PKU82_02295"
     CDS             complement(7968..8747)
                     /locus_tag="PKU82_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ81734.1"
                     /translation="MIVNNKIGITFSGGGFRGIAHIGIMQYLKELDIPIHAVSGTSVG
                     SLIGAFVAEGYAPLEILELAKREKFFTFSDLSIGNGGLFNTDIFEKVIKKYIPHDSFE
                     RLKMPLYVAVTDLTYAELLIFNQGSLSFAVKSSCCFPLVFHPVVYNNDRYLCDGGLLN
                     NFPVEQITATCNKSIGVHINPINKLEGKLGYKEIIQRIIRIATSNIKSAENHKCDIFL
                     QPDEINKFNTFDTGKIDEIYKVGYDYAKKFKKEFLELKKQV"
     gene            9007..10464
                     /gene="cls"
                     /locus_tag="PKU82_02300"
     CDS             9007..10464
                     /gene="cls"
                     /locus_tag="PKU82_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOZ81735.1"
                     /translation="MKWIFITEIIYVLIVILVCLRIIWDTGTTTKALAYLLLVIFVPF
                     IGIFFYFSFGINYRKRKMYSKKIFQDDVLAERFREQILQYSEKTYRENIDIVENNKEL
                     ARLLTYDSLSALTSGNEVKLLINGEQKFPEVLNAIASAKHHIHIEYYIFENDSIGKSV
                     IEALIAKVGEGITVRFIYDDFGSHALNRKILKRMKDGGINLFPFSRVRLFALANRLNY
                     RNHRKIIVIDGNIGFVGGINISERYINSEEQGKKLYWRDTHLRIEGAGTYYLQYLFLC
                     DWNFCAQSQLQPSREFFPSPTLLNNKGNKIVQIAASGPDSERPTILFSLLQAISLASK
                     EILITTPYFIPGESILDALMVAALGGVSVKILVPGISDSRIVNAAAFSYYDSLLNAGA
                     EIYLYQKGFIHSKTLVADDKIAMVGTANMDHRSFDLNFEVNAIVYDETFAKELKREFY
                     KDLKDAESIDVKAWNNRPIIKQLGEKCARLISPML"
     gene            complement(10484..11086)
                     /locus_tag="PKU82_02305"
     CDS             complement(10484..11086)
                     /locus_tag="PKU82_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ81736.1"
                     /translation="MNAECTPFNSNDKAVENIIVNRARHLFMKVDIKSVSMDDIANAC
                     GISKKTLYKCFDKEGLVKTVTQQQVHIFENEIKSILQNNKDVVQELTNLLLFTINASQ
                     EISLPFIDSLKTIKPEAYQLILNFTKNIQQTFINNIKKGMSQGYYRSDIEPDVVVQLH
                     FLQLWSLMKQQMLSKSLHWGEELNKMFLFGILSPKGLKTL"
     gene            complement(11099..14287)
                     /locus_tag="PKU82_02310"
     CDS             complement(11099..14287)
                     /locus_tag="PKU82_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ81737.1"
                     /translation="MKITEISINRPTMVVVVFTILGILGLISYSSLNYELLPKFSTSM
                     VTISTVYPGAGPDEVQNTVTKKIEDAVSSMENIKKLTSRSMEGVSIVTVEMNVGADAN
                     RSLEDAQRKINAMLSDLPSDAKTPTLSKVSVDDLPIITLSVTADMDSREFFDLLDQRL
                     APRLSRLPGVAQVQMIGGTEREFQINVDAGKLNAQKLSLLQLRQTITNANVDYPTGKI
                     QTTDNLIAIRLSGKYADVDMLRNLVIKTSDDGSQVRLKDVADVRDGQKDVNKLARQDQ
                     KETIVVQITKQSDANAVTVSKEVHNQIAGIEKEYASNNLKMFVASDSSEFTLESANDV
                     VFDLFIAIILVAVVMLLFLHSIRNSLIVMISIPLSLIITFAGMYVMGYSLNLMSLLAL
                     SLVVGILVDDAIVVLENIYRHMEMGKNKVRAAFDGVKEIGLTVTSITLVIVVVFLPIS
                     LTNSLVSMILRQFCITVVISTLLSLLVSFTVVPWLSSRFGKLEHLTGKNMFQKFILWF
                     ERQIDKFTEWITGILRWSLGHKRYVLIGTIVLLVASFMLLAKGFIGGEFIPKGDRGQF
                     IVTIEMPKDVALEVTNQMTMRAENYLAKQDLVKSLTTTVGKSSDGGFGTSSSTPYKSE
                     ITVTMIDENLRSEPSSMYAAKIKKELAAQLAGAKITTSPVSIMGTAERASIDLVVTGT
                     EQDSAMAFAKKALLALQKIDGVSDPKLSAEDGNPEVRVTLDRDKMALLGVNMDVVGGT
                     MQTAFSGTDQDSKLKFREGEYEYDINLRYDNFNRKNADDVRNIMFVNNKGQNIRLSEF
                     ATISETSGPTVLERRDKLASVNVQAQVIGRPAGSVTTDFTESLKKLDKPAGVNYIFGG
                     DAENQGNAFGTLGLALLLSIIIMYLIMVALYDDYVRPFVVLFSIPLSLIGALLALALT
                     NNTLNIFSILGVIMLIGLVAKNAIMLVDFANQMKEKGESTDEALVHANNARLRPILMT
                     TIAMVIGMLPIALASGGVAAIKHGLAWVIIGGLISSMFLTLIVVPVIYKIMDSVSARF
                     GWGDLNKKKIVRELMHAENTNTADNHSISHVI"
     gene            complement(14325..15386)
                     /locus_tag="PKU82_02315"
     CDS             complement(14325..15386)
                     /locus_tag="PKU82_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ81738.1"
                     /translation="MKKVFIYGGTLIVALALIVWKLNANKKSDAARIEVVRESSSGAV
                     PVQILAVKSSNVDLSFDENGNFEAVNQIELTAEIAGRVSELYVKEGSVVKAGQAIAKI
                     ENEILNANQTEAKARLEQLRLNLNRYEQAYKTGGVTQKQVDDARLELESAQAKNVQAN
                     KNLYNSVVKAPVNGIINTRYVEVGTYLASGNKIVEIVDDAKLKLTVNVNEYQIIQLHT
                     GDKVEVTASVYPEVTYQGVITFIAAKSDASLNYPVEVEINNIAGKALKAGMYGTAHFK
                     PEIKNASLMIPRSAFQGGINSNLIFINDNGKAKLQKVVAGNVHGNMVEIREGLKEGDQ
                     VIVTGQINLVDGKQISIVK"
     gene            complement(15415..16776)
                     /locus_tag="PKU82_02320"
     CDS             complement(15415..16776)
                     /locus_tag="PKU82_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ81739.1"
                     /translation="MKLSIKNNALAIAIMLFSTQVMAQTKSLNLKDLIQIGLEQNYNI
                     QLSKLDEAIVEKQIVETRGLTLPQVNGTGNITNNYKRQVLVLPAGLGGGTGEGSRKIE
                     AGTNYSSALGVEATQALFDVAALQGLKAAKAGRVYARVNTQQTQEQVVNDIAQQYYQI
                     LSSMEEVKLQHYTIEIMQQLVQASEGKYNNGLLRKVDVDRLKVNLINAQSKLTQAQND
                     VDVKTNQLKVTLGLPYNTSILLDSMKIDETSLQVALQQQQYNPDASTDLKLLDTQIKL
                     STLERNATRAANYPKLNAFANYNYNMASDKFSDLYKGTDPAISYGMGSVGVRLSIPLF
                     SGFSRSSKVAQNNIKIHQLEKQRQATAITLEAKNESARIQMENTLTTARAQQENVKLS
                     ETVYESNRSNYTLGLSSLTDLLDSQTSFLEAKNIYTKSIINYKLAELELMRSNGTILN
                     LIN"
     gene            17100..18191
                     /locus_tag="PKU82_02325"
     CDS             17100..18191
                     /locus_tag="PKU82_02325"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ81740.1"
                     /translation="MITSKTKWIEFINKYYKYFLIPIVFFTHKLLFFLTDPYAQPIRK
                     LTFQESFIQNLGLLFFSFILVYGSVRNVNRLNKRLPWAASPILRLIAQVFVQLMLSTI
                     WIFIYQFVVVKMLFDVNIFSLITSPDLTPGIRFVIWRFFFTSALVSLFVSLIITGRNF
                     RMETAEMKIQAAQLKQIAAQAELQALKLQLDPHFVFNNFSTVSALIEEDKALAQSFVE
                     NLSRVYRYMVQNIHSDTITLQREVKFIESYIYLISIRHGDNVRIKVNIPEEFLQKRIP
                     PITLQLLIENAIKHNIASHTKPLNINIGVADDMLRVSNNLQRFSNPSQISTRIGIENI
                     KSRYRIVSGKEPVVIETADDFIVELPLLD"
     gene            18204..18962
                     /locus_tag="PKU82_02330"
     CDS             18204..18962
                     /locus_tag="PKU82_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ81741.1"
                     /translation="MKFLIVEDEKPNANRLKKIVEQLDSSFQVVDIIDSVAGAVDWFN
                     KNPHPDIVLMDIRLADGLSFDIFSQVKISSAVIFTTAYDEYALQAFKVNGVDYLLKPI
                     EIEELDAAISKVKQNHISASNNPYLDELIGYIKQKEVQYRSRFLFPYKDGYKTINVSD
                     IDLIYSEDKNTHLILQNGSVQIANQTLEEMEEQLDPAVFFRANRQYIININSIESIHN
                     HFNGKLKVVVRKLPQHDILISKEKAPLFKQWLDS"
     gene            19520..20272
                     /locus_tag="PKU82_02335"
     CDS             19520..20272
                     /locus_tag="PKU82_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81742.1"
                     /translation="MLKRFVCITISLLAFIIVNAQDNKTEKKDSIEHRILLIPYDPRF
                     YLSDADKDIAEETRKDPKTIRNYFHNRSEWFTSRELKRHYPTISLLQNDTVPAYIQSA
                     GDIFSQTAFDYATPVTRSPESMMNATSDKTLTDSRTATNYLNTDVHNRYMKADIQNKK
                     VLADLSERFNTDIFVFLTQFEIKTNYKNCLDIANKIYEREIRLHFTIYDKTGKLINGN
                     YAIAVIPSNVNQMDEIILKCFPLLAKGIAASL"
     gene            20354..22600
                     /locus_tag="PKU82_02340"
     CDS             20354..22600
                     /locus_tag="PKU82_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOZ81743.1"
                     /translation="MRKLLTLSALFVILSGTQSLASSFWTAKSETQEMLSGKRQIVPD
                     KYKVYTLDVQGLKNQLLNAPMEFTMAAKQSPVVITLPMPDGSWNSFSMVESPVMQPKL
                     AAKYPFIKTYSGSGINNQPSWVRIDFTQWGFHALIRTAENDIYIDPYSMQTTSDYLVY
                     FRKDFTKSQSSMVCEFDDLQQGQSANKTLTNSMPSVNRSIGTNLRTYRLALACTGEYA
                     AYYGGTKAGALSGMVTSVNRVTGVYESEVDVRLNLIANTDTLIFLNANTDPYTNNSGG
                     TMLGENQSVVNFFIGTPNYDIGHVFSTGGGGIAYLGCICKTADKAKGVTGSAAPINDP
                     FDIDYVAHEMGHQFGGNHTFNSVTGSCQGNREAGAAYEPGSGVTIMGYAGICGSDDLA
                     AHSIAYFHTKSFDEIVDYSTLSTGNSCPVNTITFNNAPTVSGGGIFNVPVGTAFKLNG
                     YGSDPDGDSITFSWEQFDLGPAGAWSLPTGNAPMYRSYAPSTSALRLFPKLSVLLNQV
                     NAKGELITSYARSLHFRLTARDNKPNGGGVTYNDTVTTVNVVQTSAPFAITYPNVTGI
                     SWPALSTQTITWEVGGTDAAPFNEQFVNIYLSINNGTTFPITIATNVANNGSYTFTVP
                     NNPSNICRMWVEGASTGSIFFDINNKVFTITAPVGINEVNSLSQLTTYPNPADNELLV
                     YLGNNYHGQLNISLYSVNGQLVLSQTIAAKNATAQVALNVEQLPAGLYFLKAETENGT
                     AERKVMIK"
     gene            22807..26022
                     /locus_tag="PKU82_02345"
     CDS             22807..26022
                     /locus_tag="PKU82_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81744.1"
                     /translation="MSFGKNRVQYTDFYWTFFRFKNYDVYNYVGGKELATYVAEQAQN
                     EMDVMERTLDYRSSSRFQIVIFNKLSDLKQTNIGLELDDQAYNIGGMTKVIGNKVLIY
                     FDGNHQHLQEQIRAGMARALLEQLFYGGNIRERLQASILLNLPQWYTQGLVTYLSKGW
                     TPEDDNKLRDYIMTEKKPVLNKLIERDEVFAGQSLWHYIITTYGETSLSNLVYMTRIN
                     RSLESGFVFVLGVRLKQLSADWLSYHTKKYQEFEKNKFPIAATPLKVKEKNKIIYQVK
                     TDPFGNRVSYVTNKRGRYKVKITDVKTGKRTCADKGGYVSLTTENDRSFPITAWHPSG
                     KILAIVKEQKGNIILNLFNTETKKSDFSEIMSFSKILNMTYSPDGSKLLMSAVQKGQS
                     DIYVFNVRGRNFDQITNDVYDDLYPQYDITGNKIIFSSNRSNDTLGVDVKQNTPQNNF
                     DLFMADLYNREGNLLRITHSPENETFPVMSDSLRISYLSDKNGVINRFVSEMDSTISF
                     IDTTEHYRYFFRHYALTNSNRNILEQDFSARKNKFTELAVNNGRYRIYLKDNMPFDKS
                     KTFDLSYIAQPVFQPKQENKPKVAETPTRVVTIEPSQKKDSAAIDVGNYVFQSEFKSK
                     KKKEEPIENKSVATDTTKIVQEQVKLQPDSNDVKSTPDSAIFRMPKQRNYDISFSSDY
                     ILTQLDNSLFNTTYQPYTGGAVYFDPGMNALFKIGMSDLMNDYKISGAFRIGFDFNSN
                     EYMLMIESLKKRIDKQILFSRQSKLFSVSDLDVLRTHTYNLKYSGKYPFSEISAVKGT
                     IDARMDKTIYVATEKAQLEKSDITNYWASIKGEYIFDNTINKALNLYNGTRFKIFAEV
                     FRKINKPEKMVYIIGADFRHYIKVHRQIIWANRLSASTSLGKQKLIYYLGSQDNVIVP
                     GNNFNNEISVDKNEEYVFQTLASPMRGFKQNIRNGSSFALFNSELRIPVFQYLLNRPI
                     RSDLIRNFQAVVFFDAGTAWSGTSPFSDKNGLNEEVIGGGGSNPVTVTVIKQIQPVVG
                     AYGFGFRSRIFGYFIRADWGFGIDDRQVQKPLFNLALGLDF"
     gene            complement(26023..27285)
                     /gene="rodA"
                     /locus_tag="PKU82_02350"
     CDS             complement(26023..27285)
                     /gene="rodA"
                     /locus_tag="PKU82_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOZ81745.1"
                     /translation="MRKQKSVFQNIDWLLVLLYLVMVIAGWLNIFAAVYNEEHSVITD
                     FSQNYGKQMMWILTSLVIAFFILIIDGKFYDAFAGVIYAISIISMIAVLFFGHTVAGS
                     KSWFRFGDFGIQPAEFAKFATAMMIAKFLSPMGLKKNTWRTRIYAVAIFFIPLLLILL
                     ENETGVALVFLSFCIVLYREEIIPGWLLMIGISAAVLFVVSLLVPKMILVTSLLVLAA
                     LIVLLFRKWKTFFVVAGLVAICIGVVVSVDHVFNHVLEPHQKKRIQVFLGMEEDLKGA
                     GYNVNQSKIAIGSGGLTGKGFLKGTQTKYNFVPEQSTDFIFCTVGEEWGFLGTTTVIL
                     LFLALLYRIIFIAERQKSAFARIYGYGVAGVIFFHFMVNVGMTIGLMPVIGIPLPFFS
                     YGGSSLWGFTILLFILIKLDSYRTFVLR"
     gene            complement(27289..>28530)
                     /locus_tag="PKU82_02355"
     CDS             complement(27289..>28530)
                     /locus_tag="PKU82_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOZ81746.1"
                     /translation="GTRYMMVDNFNREKGRFKEGKYDTLAISGENLKLSLDAELQEYA
                     ELLFQNKRGSAVAIDPSTGEILAMVNSPTYNPNLMVGRERSRNYGRLLKDEDKPLFNR
                     AMMAYYPPGSTFKIINSLIGLQEKVITPETYFSCAGGYRVGNIFVHCHPHSSPLDLRT
                     AVAVSCNSYYCQTFRGVIDNRKYNRTEDAFDAWRNYVLSFGIGKKIDVDMPHELRGNV
                     PTVDYYNRFFGKGRWKSSTIISLAIGQGELGITPLQMANAACIVANKGYYYVPHIVKE
                     IGYKHYLPELFKKKNYTLVDPKYFDAIQDGMEWVFERGTARGSRVDSLVMCGKTGTAQ
                     NPHGKNHSVFFCFAPRENPKIAVAVLVENAGQGAHFAAPIASLMVQKYLLRTIKRPDL
                     EKRMLDAVIVPMANDSTVLDD"
BASE COUNT         9206 a         5164 c         5013 g         9147 t
ORIGIN      
        1 agattacatc atccgttttc aaaacacagg aaacgataca gcatttaacg ttaaagtttt
       61 aaatccgtta gatacaaata aacttcagtt aaacacctta gaatatgttg catcatcgca
      121 tcctgtaaac atgaatttta tatatcatga gcgcaacatg gagtttaagt ttgataatat
      181 tctattgccc gatagtaatg taaacgagcc gctaagtcat ggctttattc attacagaat
      241 aaaaccaaaa acaagtctta ttttaactga caccattaaa aacactgctg ccatttattt
      301 tgattttaat gaccctgtaa taaccaacac agcaacaacg catgttgtac taccaacagg
      361 aatagcatca tatcaaagtg cagaaaacat acaattattc ccaaatcctg ctcaatctga
      421 attaaatatt cagcaaatag cagcagttga aaaaatacaa gtaatagacg tacttggaaa
      481 aattgtgttt gaaaagaaaa tcaaaagcca gcaaaattta aagataaaca tcagtcaatt
      541 tcaaaacgga gtttattttg taaaggcata cagcgcacat gatgtggtag tcaagaagtt
      601 tgttaagagg taggcgattt ttgtattgtt gatcagccat tgctcagccg cggttgaggg
      661 gatttgaata ttattgtcag aagttcttca attttaaatc ggtggccaca ataatttttg
      721 aagataagta aaataaattt ctaaatcaga cagcttttat ttattctctt attctgataa
      781 aaatataaac caaactattt tttatctcat ttgaattaca tacttttgat ataaccaaaa
      841 aagtaagtaa catgagaaaa ctgattatat tactcttgat ttgtacaact accgcccatg
      901 cccaggaaat tgaatggcaa aatactattg gaggaagtgg tgtagaattt ttaaattaca
      961 cacaacaaac ttcagatggt ggctatattc ttgggggtcg ttccaattcc ggtgtttccg
     1021 gtgataaaac agaaccttca aaaggagatt atgattattg ggtggtaaag ttagacgcaa
     1081 ctggaaatat tcaatggcaa aacaccattg gtggagatga tgctgattgg ttacaatcgg
     1141 ttcaacaaac tacggataat ggatatattc ttggaggatg gtctaagtca aacatttcag
     1201 cagataaaac agaaaattca caaggggctt ctgatttttg ggtggtaaag ttggattcaa
     1261 taggtaacat tcaatggcag aacaccattg gtggtgattc taccgataat ttatattctt
     1321 tacaacaaac taccgatggt ggttacattc taggaggatt ttctatatct tctatttcag
     1381 gcgataaaac tgagtattcg ataggtggtt ctgattattg ggtggtaaag ttagatgcat
     1441 caggaaacat tcagtggcag aatactatag ggggatacga gaatgataat ttatattctt
     1501 tacaacaaac cacagatggt ggatatattc ttggcggacg ctctatgtct aatgctacag
     1561 gagataaaac agaaaattca caaggacttg atgattactg ggtggtaaag atagatgcaa
     1621 ctggaaatat tcaatggcaa aacaccatag gaggaaatag ctgggatgaa ttagaatcta
     1681 tagaacaaac ctcagatggc ggctatatat taggtggttc ttccgattct gacaattccg
     1741 gtgataaaac tgaaaattca caaggagatt ttgattactg ggtggttaaa ttagatgcaa
     1801 ccggtaattt acaatggcaa aacactattg ggggcgataa ttgggacaag ttgaagtcta
     1861 ttcaacaaac tactgatggt agttatattt ttggggggta ttcttcttct aatatttccg
     1921 gtgataaaac tgaaaactca caaggaaact atgattactg ggtgataaac atagattcaa
     1981 taggtaatat actttggcag aatacgattg gaggtcttta tgcagaagca ttaagctcaa
     2041 taaaacaaac tattgatggc ggatatatta ttggagggca atctgtttct cctatttcag
     2101 gcgataaaac agaagattca caaggtaata atgattattg ggttataaaa ttgacagata
     2161 aatataattt aattgatggc aaaatgtatg cggatgtcaa taacaatacc atacaagatg
     2221 ttggtgaatt gggtgtatct aacaaaatgg ttactgaaca aaatactgga agattatctt
     2281 tctcacaaca gaatggaaaa tatagtttat cggtgttaga tacaggtggt ttttctgttt
     2341 ctgcatcaac gataaattat tacaatagtg tcccattagt tcatactgct tattttaaca
     2401 acattaatca aactgattca ttaaacgatt ttgcattaca gcctaatgga gttattaacg
     2461 atgtatgtat aaatcttaca ccagtttctt tattccgctc aggttttgat gcaacttact
     2521 atatcagcta tattaacaat ggtactacaa cacttacgcc aactgtaatt ttctttcctg
     2581 atgcgaatgt ttcttttaca agtgctaatg taactcctac ttctgttaca accgattctg
     2641 tcgtatggaa tattggcgca cttgcacctt ttcagtcagg aagtattgtt gttacagtaa
     2701 atgtacatac gggtttgccc attggttcac ttatcaattc aagtgtaaga atagagcctg
     2761 ttgccggtga tgctaatccc ggttgtaatt acagttcttg ggaggttttt accacaggtt
     2821 catttgaccc caatgatatt ttagtaagta gagatacttt attcactaca gaaattccca
     2881 atccacccta tttagattac atcatccgtt ttcaaaacac cggaaacgat acagcattta
     2941 acgttaaaat tttaaatcca ttagatacaa ataaacttca gttaaacacc ttagaatatg
     3001 ttgcatcatc gcatcctgta aacatgaatt ttatttatca tgagcgcaac atggaattta
     3061 agtttgataa tattctattg cccgatagta atgtaaacga gccgttaagc catggcttta
     3121 ttcattacag aataaaaccc aaaacaagtc tggtattgaa tgatagcata caaaacaatg
     3181 ctgccattta ttttgatttt aatgaccctg taataaccaa cacagcaaca acacatgttg
     3241 tactaccaac aggaatagca tcatatcaga gtgcagaaaa catacaatta ttcccaaatc
     3301 ctgctcaatc tgaattaaat attcagcaaa tagcagcagt tgaaaaaata caagtaatag
     3361 acgtgcttgg aaaaattgtg tttgaaaaga aaaccaacag ccaacaatat ttaaagataa
     3421 acatcagtca atttcaaaac ggagtttatt ttgtaaaagc atacagcaca caaggtgtgg
     3481 tggtaaagaa gtttgttaag aggtaagaga atagttgaat tactgatcag ccacggctca
     3541 gctgaggctg aggagatttg gtgaacttta caggcaaaat ttcagatttg tttttgaaat
     3601 gcagttgtcg tttaatcttc ttctgatttt aagaaattaa gatttctctt tatccctttg
     3661 tatcctgttc gttgcagcgc agagtcttta aatatttttt taaaaatttc ttctgtaagt
     3721 tctgtccatt catgctttgt catttctatc aattcacttt taggctgcag ttcctgatgt
     3781 tgatgtggtt ttgcaaaacg gttccaagga catacatcct gacaaatatc acaaccaaac
     3841 atccagtttt taaattggtt ttggaaagat acaggaattt cgttcttcaa ttcaatagta
     3901 agatatgaaa tacatttgct gccatcaaca acatgaggca ttacaattgc ctctgtcggg
     3961 caggcatcaa tacattttgt acaagtgcca cagtaatctt ttattggagc atcggcatca
     4021 agttcaatat caagaatcag ttctgcaata aaataccagg aacctgattg cggatttatg
     4081 agattattat tttttcctat ccatcccaat ccacttttac gcgcccaggc tctgtctaac
     4141 acaggtgcag agtcaacaaa aacacgtccg tcaattgctc ctatatgctg tcgcataagt
     4201 tcaagtagtt gaaacagttt ttctttgatt acaaaatgat aatctgttcc ataagcatat
     4261 ttagaaattt gcggtgcctg tggattttgc tttttttcag gatagtaatt aaaaagtaag
     4321 gagatgacac tcctagcgcc ttcaaccaat tttgttgggt ctaaacgctt atcgaaattg
     4381 cgttccatgt acgacatggt gccatgcatg tttgctgtaa gccattgttc gaggcgtggt
     4441 gcttcttctt ccagaaaacc ggctttggaa atgccgcaat gtgagaagcc caactttaat
     4501 gcttcggctt tgacccatgc tgttgtctgg ctacggttca ttgtttttta agactcagag
     4561 tcgaacaatg ttcccttctg ctttaaagac tttttaccaa gatgtttaaa agctttttct
     4621 gttgcttcac gtccgcgtgg tgtacgcatc aaaaaacctt ccataattaa aaacggctca
     4681 tacacttctt caatagtacc tgcttcttcg ccaacagcag tggcaatagt gttcaatcct
     4741 acaggtccac ctttaaattt ttcgataatg gcattaagaa tcctgttgtc catttcgtcc
     4801 agaccgtcaa tgtcaacatt gagtgcctga agtccataac gtgcaatttc aatatttacg
     4861 gttccgttac cttttatctg tgcaaaatca cgaatgcgtc tcaacagtgc attagcaata
     4921 cgaggagtac cacggcttct tcgggcaatc tcaaaggccg cttcttcgtc aatagcagtt
     4981 ttcagaatag atgcagagcg cattacaatg tcttgtaata atcgtgcatc gtaatattca
     5041 agtcgcgagt tgataccaaa ccgtgcacgc aatggcgaag taagcaatcc tgaacgtgta
     5101 gtggcaccaa tgagtgtaaa tggatttatt ttaagctgaa ccgaacgtgc atgaggtccg
     5161 ctttcaatca taatatcaat tttataatct tccattgcag aataaagata ttcttccaca
     5221 atagggctta agcggtgaat ctcatcaata aacaacacat cgttagcttg cagattggtg
     5281 agtaagcctg caaggtcgcc aggtttgtca agcacaggac ctgaggtaac ttttattttt
     5341 acattaagtt cgtttgctat aatgttggct aatgttgttt tacctaagcc cggagggcca
     5401 tgcagtaaaa catgatcaag agcttcgcca cgctgtactg ctgcttttac aaatacagta
     5461 agattttctg ttaccttacg ttgtcctgca aaatcatcga agctaagcgg tcgtaatgcc
     5521 cgttctattt ccttttcggc tgtgcttaaa ttttcacttg ccggatcaag atttttattg
     5581 atactcatta ccacaaaagt aaaatttttg ggagacatag aaagcacata agttacttaa
     5641 atcctcaatg cacattgtac tatacaatgt gttctgtcat tctaaagcag atatatttat
     5701 tagacatttg aaacaaaatg gtaaaaagat gaaggggaaa ttgtggagga aacagaaaat
     5761 tattattttt agacaacgta tttttattgg aaaacgtctt gatattaatg ttgctatgaa
     5821 gcgtttaatt gctgtacata tttttatgct gctctgtagt gcaggtcagg caagtcatat
     5881 tatgggcggt gattttacct atacactcat aggaagtgta ccacagcaac agaaaaaggt
     5941 ttacaagata agcctcaatt attaccgtaa ctgcgatact tctgctgcac aatttcaacc
     6001 atattatgat ctttatattt gttatgattc ttctaatgcc aactataaac gagcgtatgc
     6061 acaaatttcg ctggataaaa taagcacaca gccaattgtt ccgcctatat tagacactac
     6121 ttgttattat aacaacaatt ggtgcgtaga aaaaacatac tacgaagcga ctgttacgct
     6181 gtcagaagat acaacctggc acttgttttt aagtgatgga gcaagaaatg ttagtatcaa
     6241 caacctgatt caaccggata gcacaggcat atttttttat acagttattc cggcaggcat
     6301 ctccaactct acaccacagt tttcaaattc gcctacacct ttcatctgta atttagatac
     6361 gatgcagcta agtaatctgg catttgacag tgatggagat agtttggttt ataaaattgt
     6421 gcagccttat ggatacatag gtttaaacaa tttaatgacc tatcaggata ttaattttaa
     6481 cttgtattat ttgtttcctt taccaacagt catttacggc ccgggctata gttacactag
     6541 tccttttggt gcaggaggtc tctgtagtat aaattcacaa acaggattgc tgactatctc
     6601 ttcaccaaat cagggattat atgttttggc catagaagta gaagagtata ggaatggaac
     6661 acttctttcc agaacaaggc gcgacatgca ggtagtggtt gctgcttgct cctataatac
     6721 ggcaccacaa ttaacctcac ctgttgtttt aaattatcag atagatgaag gagattcaat
     6781 ttgctttccc ctcacattta ctgatgcaga aaacaatatg ataaacattt cggcaacagg
     6841 taatcttttc ggtacagcac tcaccaatcc acctgcacag tttgttgtta atccaactcc
     6901 aaacaataat acagctcagt tttgctggaa aacaatttgc ggacaggcag caccattccc
     6961 ctatatgttt acagtagaat caacagataa tggttgtcct tcaaaaacca acacctatgt
     7021 tttttctata cgtgtaaaga ctattcctca caaccaactg ccttccataa acctgagtat
     7081 gtatcctgat acacctacct gcaccggtac agcctttcag tttaatgcct ccacacaatt
     7141 tgccggaaat aaccctactt atcaatggta tattaacgga caagcactat ctaataatta
     7201 tcctaactac acaacgaatc aacttaacaa tggtgatagt gtatcggtgc aactcatcag
     7261 caattctatt tgtgtaattg attccagtgc aatttccaat acacaagttg caacagtgct
     7321 gcaaccgaca gttgcttcat tcattacaag cacggtttcg gttagtagta tcaatgtgct
     7381 gcaacaattt acaaaccaat cggtgggtaa cacttataat ttctggaact ttggagacag
     7441 tacacaatct gctgaaataa atccgcaaca tgtttatgaa aacagtggtg tttatttggt
     7501 acaactcgta acagttagtg atgatggttg tcgtgataca acagaagcac cggtggatat
     7561 caaaacaaca atgagttttt ttatgccctc ctcatttaca cctgacgata atgggttgaa
     7621 tgatttgttt actccgatag gagcagaaat gccggattat acgcttcaga tatttaaccg
     7681 ttggggacaa ttactttatt ctgaaactgg aaaaccgcaa tggaatggtc gttataaaaa
     7741 tcaactacta cctttaggaa tgtacattta ctccattcgt atgcacaatc caacagatcc
     7801 cattattaaa accggaacag taatgttgtt gcgatgaaaa ttatatttta tttgttggaa
     7861 ttctatctta taaattttta aatttccgat attaattcac caactgtatc tactggagaa
     7921 ataagtattt tacgtaatga atttatttta catgaaacct ttactactta tacttgcttt
     7981 ttaagttcta aaaattcttt tttgaacttt ttagcataat catatcctac tttgtaaatt
     8041 tcatcaatct ttccggtatc aaaggtgtta aatttattga tttcatcagg ctgaagaaaa
     8101 atatcacatt tgtgattttc tgcactttta atatttgaag tggcaatacg aataattcgt
     8161 tgtatgattt ctttataacc taatttacct tctaatttat taatcggatt aatgtgaaca
     8221 ccaatactct tattacatgt tgcggtaatt tgttctactg gaaagttatt caacaaccct
     8281 ccatcacata aatatcggtc gttattatag acaacaggat gaaacaccaa aggaaagcaa
     8341 caagaagatt taacagcaaa ggataaacta ccctgattaa aaatcaacaa ctctgcataa
     8401 gttaaatctg taacggcaac atataaaggc attttcaatc tttcaaaact atcatgtgga
     8461 atatatttct taattacttt ttcaaatata tccgtattga acaaaccacc attaccaata
     8521 gacaaatcag agaaagtaaa gaatttttca cgcttagcta gttctaatat ttctaaaggg
     8581 gcataacctt cagccacaaa agctccaatc aaagagccta cactcgtacc cgatacagca
     8641 tgaataggaa tatctaactc tttcagatat tgcataatcc caatatgtgc aatcccacgg
     8701 aatcctccac ctgagaaagt aattccaatt ttattgttta caatcataat ggttaactca
     8761 taaaatttat agcaaatata aagctatccg atcaaagtaa taaaatgtca aagtatgtaa
     8821 taagatgcat tcattaggga atctgtttag tgtacttgct ttacaatatt atatttgtaa
     8881 aaacaaaaat tcttgaaaca aaaacaaatt agtatgcaga gtatttacga aagtaaattt
     8941 tacaagtatc tgttttatat taatcagcgg acacaactat agaatttaaa atgtcatttt
     9001 gttattatga aatggatatt tattacagaa attatttatg tacttatagt tatactggta
     9061 tgcttacgaa ttatttggga taccgggacc actactaaag ctctggcata tcttttattg
     9121 gtaatctttg tgccattcat tggaatattc ttttattttt cttttggtat taattatagg
     9181 aaaaggaaga tgtacagtaa aaaaatattt caagacgatg tgttggctga aaggttcagg
     9241 gagcaaattc ttcaatattc agaaaaaaca tatagagaga atatagatat agttgagaac
     9301 aacaaagaac ttgcccgact tctgacctat gatagtttaa gtgctttaac ttcaggcaat
     9361 gaagtaaaat tattgattaa tggagagcaa aaatttcccg aagtccttaa tgcaatagca
     9421 tcagcaaaac accatattca tattgaatat tacattttcg aaaacgatag tattggtaag
     9481 agtgttatag aagctttaat agctaaagtt ggcgaaggaa taacggtgag gtttatttat
     9541 gatgactttg gaagtcatgc attaaatagg aaaatactga aacggatgaa agatggcgga
     9601 atcaatcttt ttcctttttc aagagtaaga ttatttgcat tagccaatcg ccttaactac
     9661 agaaatcatc gaaaaattat tgtaatagat ggtaatattg gatttgttgg cggaattaat
     9721 attagtgaac gttatataaa ttcagaagag caaggtaaga aattgtattg gcgcgacaca
     9781 catctgagaa tagaaggtgc aggcacatac tatctgcaat atcttttttt atgtgattgg
     9841 aatttctgtg cacagtcaca attgcaacct tcaagagaat tttttccatc accaacttta
     9901 ttgaataaca aagggaataa aattgttcag attgcagcaa gtggaccgga ttctgagcga
     9961 cccacaattt tattttcatt actacaggct atcagtctgg cctcaaaaga aatattaatt
    10021 acaacacctt attttattcc gggagaaagt attttggatg ctttgatggt agctgctctt
    10081 ggtggagtga gtgttaaaat tcttgttccc ggtatttctg attcaagaat agtaaatgca
    10141 gctgcatttt catattacga tagtctttta aatgcaggtg cagaaattta tttgtaccag
    10201 aaaggcttta ttcactctaa aacattggtt gctgatgata aaattgcaat ggttggaaca
    10261 gcaaatatgg atcatcgtag ttttgacctc aattttgaag tcaatgctat tgtatatgac
    10321 gaaacatttg cgaaagaact aaaacgtgaa ttttataaag atttaaaaga tgctgaaagt
    10381 atagatgtga aagcgtggaa caaccgccct attattaaac aattgggaga aaaatgcgca
    10441 cggttaattt ctccaatgtt ataatcatta aaaagcaaag gcactataaa gtttttaacc
    10501 cttttggaga taaaatacca aacagaaaca ttttatttag ttcttcaccc caatgtaaag
    10561 atttgctaag catttgttgt ttcattaaac tccatagttg caggaagtgc aactgtacga
    10621 caacatcggg ctcaatgtcg gaacgataat atccttgcga catacctttt ttaatattat
    10681 ttataaaagt ctgttgaata ttttttgtga aatttagtat cagttgatat gcttccggtt
    10741 ttattgtttt tagtgaatca ataaagggaa gagaaatttc ttgcgaagca tttattgtaa
    10801 ataaaagaag gtttgtcagc tcctgtacaa cgtctttgtt attttgcaag attgatttaa
    10861 tctcgttttc aaatatatga acctgttgtt gtgtaacagt ttttaccaaa ccttctttat
    10921 caaaacattt atatagtgtt tttttggaaa tgccacatgc atttgcaata tcatccatac
    10981 ttactgactt tatatctacc ttcatgaaca aatgacgcgc tctattgaca attatatttt
    11041 caactgcttt atcgtttgaa ttaaatggag tgcattcagc gttcatgaat gtttatgctt
    11101 aaattacatg agatattgaa tggttatctg ctgtatttgt attttcagca tgcatgagtt
    11161 cgcgtactat ttttttctta ttcaaatcgc cccaaccaaa acgagcagaa acgctatcca
    11221 taattttata aataaccgga acaactatta atgtcaggaa catggatgaa attaaccctc
    11281 cgataattac ccaggccaaa ccatgtttga tagcggctac accacctgat gccaatgcaa
    11341 taggaagcat accaattacc atagcaatgg ttgtcattaa aattgggcgg agacgtgcat
    11401 tgttggcatg taccaaagct tcgtctgtag attctccttt ttctttcatt tggttagcaa
    11461 agtcaaccag catgatggcg ttttttgcca ctaaaccaat caacatgata actcccagaa
    11521 tactgaaaat attgagtgtg ttattagtga gtgctaaagc gagcaatgca ccaatcagag
    11581 aaagcggaat agagaacaat acaacaaacg gacgcacata atcatcatac aaagcaacca
    11641 taatcagata catgataata atagaaagta ataatgccaa acctaatgta ccaaatgcat
    11701 ttccctgatt ttcggcatcg ccaccaaaga tgtaattgac accggcaggc ttatccaatt
    11761 ttttcagact ttcggtaaaa tcagtggtta cagaaccagc aggtctgcct attacttgtg
    11821 cctgtacatt tactgatgca agcttatctc tgcgctccaa tacagtaggt cccgaagttt
    11881 cagatatagt agcaaattct gacaatcgaa tgttctgacc tttattgttt acaaacataa
    11941 tgttgcgcac atcatcagca ttttttcggt tgaagttatc gtagcgcagg ttaatatcat
    12001 attcgtattc tccttcgcgg aatttaagtt tactatcctg atcggtacca ctaaaagctg
    12061 tttgcattgt gccgcctaca acatccatat tcacacccag tagagccatc ttatcacgat
    12121 caagtgttac tcgcacctcg gggttaccat cttctgcaga taattttggg tcagacacac
    12181 catcaatttt ttgtaatgca agtaatgctt ttttagcaaa agccatagcg ctatcttgct
    12241 cggttccggt aacaaccaaa tcaatagaag cacgttcggc agtacccatg atgcttacgg
    12301 gagaagttgt tattttagct ccggctaatt gtgcagctaa ttcctttttg atttttgcag
    12361 catacatgct tgaaggctcg cttcgcagat tttcatcaat catagtcact gtaatttctg
    12421 atttgtaagg tgtagatgat gaagtgccaa atcctccatc gctgcttttt cctactgttg
    12481 tggttaatga ctttaccaaa tcctgtttag ctaagtaatt ttcagcacgc atagtcattt
    12541 gattggttac ttccaaagca acatctttag gcatttcaat ggttacaata aactgtccgc
    12601 gatctccttt aggtataaac tctccaccaa taaacccttt tgcaagcaac ataaaagatg
    12661 caaccagcaa tacaattgta ccaataagta catagcgctt atgaccaagc gaccaacgta
    12721 atatgccggt aatccattct gtaaacttat ctatttgacg ttcgaaccac agaataaatt
    12781 tttggaacat gtttttacct gtcagatgtt cgagttttcc aaagcgtgat gacaaccaag
    12841 gaacaacagt aaacgacacc aacaaactta acagtgttga aattactact gttatacaaa
    12901 attgacgcaa tatcattgac actaaactat tggttagtga tattggaaga aatactacta
    12961 caattactaa tgtgatggag gtaacagtaa gaccaatttc ttttacacca tcaaaagcag
    13021 cacgaacttt atttttaccc atttccatat ggcggtaaat attctccaac accacaattg
    13081 catcatcaac aagaataccc accaccaagg aaagggcaag caaagacatg aggttaagtg
    13141 aataacccat tacatacata ccggcaaatg ttattatcaa tgacagcgga atggaaatca
    13201 ttacaatcag cgagtttcgt atgctgtgca agaacaacaa cattacaacc gctaccagaa
    13261 taatggcaat aaataaatcg aatacaacat cgttggccga ttccagagtg aactctgatg
    13321 aatcagatgc tacgaacatt tttaaattgt tacttgcata ttctttctca attccggcaa
    13381 tttgattgtg tacttcttta ctaacggtta cagcattagc atcagattgt ttggtaattt
    13441 gtactacgat tgtttctttc tgatcctgac gtgccaattt atttacatct ttttgtccat
    13501 cgcgtacatc tgcaacatct ttcaagcgta cttgcgaacc atcatcacta gttttaatta
    13561 ccagattgcg caacatatcc acatcggcat attttccgga aaggcgtatg gctatcagat
    13621 tgtcggtggt ttgaattttg cctgtgggat aatcaacgtt ggcattggta atagtttgtc
    13681 gtaattgtaa caacgatagt ttttgtgcat taagtttacc ggcatctaca ttgatttgaa
    13741 attcacgctc agtgccacca atcatttgta cttgtgctac acccggtagt cgtgaaagac
    13801 gaggtgccag ccgttgatct aaaagatcaa agaattcacg cgaatccata tcggcagtta
    13861 ctgacaatgt gataataggt agatcatcaa cagaaacttt actgagtgtt ggtgttttag
    13921 catcacttgg caaatccgac aacattgcat ttattttacg ttgtgcatct tccaaacttc
    13981 tgttggcatc tgctcctaca ttcatttcaa ctgtaactat agaaactcct tccatagagc
    14041 gtgatgtaag tttctttatg ttctccatag aagatactgc atcttcaatt ttctttgtta
    14101 cagtattctg aacctcatca ggaccggcac ccggatatac cgtgctgatg gttaccattg
    14161 aagtactaaa tttaggcaat aactcataat tgagagagct ataactgata agacccagta
    14221 tgcctaatat tgtaaaaacg accacaacca ttgtaggtct gtttattgat atctctgtaa
    14281 ttttcattta ttcgattttt aaatgttagc ttttaaaaca gaacttattt tacaatagaa
    14341 atttgtttgc catctaccag attaatttgt cctgtaacaa ttacctgatc tccttctttt
    14401 agtccttcac gaatttcaac catatttccg tgaacatttc cggcaactac tttttgcaat
    14461 tttgctttgc cattatcatt aataaaaatt aagttactgt taataccacc ttgaaatgca
    14521 gagcgcggta tcattaaaga tgcatttttt atttcaggct taaaatgtgc tgtaccatac
    14581 atgccggctt ttagtgcttt gcctgctatg ttattaattt ctacctcaac agggtaattc
    14641 aatgaagcat cacttttagc agcaatgaaa gtgattacac cttgatatgt tacttcagga
    14701 tataccgatg cagttacttc tactttatca ccagtgtgca attgaataat ctgatattca
    14761 tttacattaa cagtgagttt taattttgca tcatcaacaa tttcaacaat cttattgccg
    14821 gaagccagat aagttccaac ctctacataa cgggtattaa taattccatt tacaggagct
    14881 ttcactacag agttataaag atttttattt gcctgaacat tttttgcttg tgcactttct
    14941 aattccaaac gggcatcatc aacttgtttc tgtgttacgc ctcctgtttt atatgcttgc
    15001 tcataacgat ttaagtttaa tctcaactgc tccaaccgtg cttttgcttc tgtttgattt
    15061 gcattcagaa tttcattttc aatttttgca atagcctgtc cggctttcac tacacttcct
    15121 tcttttacat ataactcact aaccctacct gcaatttctg ctgttaactc aatttgattt
    15181 acggcttcaa aatttccgtt ctcgtcaaat gacaaatcaa cgttagatga tttaactgct
    15241 aaaatttgta caggaacggc tccacttgag ctttcacgca ccacttcaat gcgtgctgca
    15301 tctgattttt tgtttgcatt tagtttccaa acaataaggg caagtgccac tattaatgta
    15361 cctccgtata taaatacctt tttcataatt tattattctt tattttaatt gactttagtt
    15421 gataagattg agaattgtac cgtttgaacg cataagctca agctcggcaa gtttatagtt
    15481 tattatgctt tttgtataaa tatttttagc ttccagaaaa gaagtttgtg aatcgagtag
    15541 gtctgttagt gaacttaaac ctaaagtata gttgcttctg ttagattcat aaacagtttc
    15601 agatagtttc acattttctt gttgtgcacg agcagttgtc aatgtatttt ccatttgaat
    15661 gcgtgcactt tcatttttag cttcaagagt tattgctgtt gcctgtcttt gtttctcgag
    15721 ttgatgaatt ttaatattgt tctgtgcaac ttttgaagaa cgactaaaac cgctgaataa
    15781 tggtattgat aaacgcactc caacactacc cataccatag ctaatggcag ggtcagttcc
    15841 tttatataag tcagaaaatt tatcgctggc catattatag ttgtagtttg caaaggcatt
    15901 tagttttgga tagttggctg cacgtgttgc attacgttca agagtactca attttatttg
    15961 cgtgtctaat agcttcagat ctgtgctggc atcaggatta tattgttgtt gttgcaatgc
    16021 aacctgtagc gatgtttcat ctattttcat actatctaat agtatagagg tgttgtatgg
    16081 cagacctaaa gttaccttaa gttgatttgt ttttacatca acatcgtttt gtgcttgcgt
    16141 taattttgat tgcgcattga tgagatttac ttttagccga tcaacatcta ctttacgaag
    16201 taaaccatta ttatactttc cttcggatgc ctgaacaagt tgttgcataa tttcaattgt
    16261 ataatgttgt agtttaacct cctccatgct actaagaatc tgataatact gctgagcaat
    16321 atcattgaca acttgttcct gcgtttgttg agtatttaca cgtgcataaa cccggccggc
    16381 tttggctgcc tttaaaccct gaagtgctgc tacgtcaaaa agtgcttgtg tagcctccac
    16441 acctaaggca gagctataat ttgttccggc ttcaattttt ctggatcctt cacctgtacc
    16501 accacctaat cctgccggta gtacaagaac ttgtcttttg tagttgtttg ttatattacc
    16561 tgttccgttt acctgaggaa gtgtcaggcc acgagtttct actatttgtt tctcaacaat
    16621 ggcttcatcc aatttgctta attgtatatt atagttctgc tctaatccaa tttgtattaa
    16681 atcttttaaa tttaatgatt tggtttgtgc cataacttgt gttgaaaaaa gcataattgc
    16741 tattgcaagt gcattgtttt taatacttag cttcatgttt atttgatttt tgcaatttat
    16801 ttgagtgtct ttgaatacgg ggtcaaaagt gcatgtattt taatgtatat aggtatctaa
    16861 attgacttaa accgctaaaa tagccgctga aacgacttag taggaaactg aaatttttat
    16921 gcttaaaaac ctgttaaata atggatggat aaacttgaaa tcgtttcggt aaggtaattg
    16981 gcagtttcag ttttatattt agcatgttgg gtcggtaaag gcaatcaata ttcgcggtaa
    17041 tttatttgtt ttgctgaaca attacgcatt cttctaatga ataagataga acatagaaaa
    17101 tgattacctc aaaaacaaaa tggattgaat ttattaataa gtactataaa tactttttaa
    17161 ttccaattgt tttttttacc cataaattat tgtttttttt aaccgatcct tatgcacaac
    17221 ctattcgtaa acttacattt caggaaagtt ttattcaaaa tttagggctg ctattttttt
    17281 cattcattct ggtttatggt agtgttagaa atgttaacag actaaacaag cgattaccat
    17341 gggctgcatc accaatactt cgtttaatag ctcaagtttt tgtgcaactc atgttgtcaa
    17401 caatatggat atttatttat cagtttgttg ttgttaagat gttatttgat gtaaatattt
    17461 tttctctcat aacatcacct gatcttacac cgggaatacg gtttgttatc tggcgcttct
    17521 ttttcacatc agcattggta tcattgtttg taagcctgat tattacaggt cgtaattttc
    17581 gaatggaaac tgctgagatg aaaattcagg cagcgcagct taaacaaata gcagcacaag
    17641 ccgaattaca ggcattaaaa cttcaacttg acccacattt tgtgtttaat aatttcagca
    17701 cagtatcagc tttaattgaa gaagataaag cactggcaca atcttttgtc gaaaatctgt
    17761 cacgtgtgta tcgttatatg gttcaaaaca tacatagcga tacaattact ttgcaaaggg
    17821 aagttaagtt tatagaatcc tatatttatt taatcagcat ccgtcatggt gacaatgtgc
    17881 gtataaaagt taatatcccc gaagaatttc ttcagaaaag aattccgcct ataactttgc
    17941 aactcctcat cgaaaatgca atcaaacaca atattgcttc acatacaaaa ccattgaaca
    18001 taaatatcgg tgtagcagat gatatgttac gcgtaagtaa taatttacaa agattttcaa
    18061 atccctcaca aatttcaaca cgtattggta ttgaaaatat caaatcacgc tatagaattg
    18121 tgtcaggaaa agaaccggtt gttattgaaa ctgccgatga ttttatagtt gaactcccat
    18181 tattggatta aaatagttgt atcatgaaat ttttaattgt tgaagacgaa aaaccaaatg
    18241 ctaaccgact gaagaaaatt gttgagcagt tggatagctc atttcaggtt gtagatatta
    18301 ttgactccgt agcaggagca gtggactggt ttaataaaaa tccacatccg gatattgtat
    18361 taatggatat tagattggcg gatggattga gttttgatat tttcagtcag gtaaagatta
    18421 gcagtgcagt aatttttact actgcttatg atgaatatgc tttgcaggct tttaaagtaa
    18481 atggggttga ttatttacta aagccaattg aaattgaaga attggatgct gcaatatcaa
    18541 aagtaaagca gaatcatatc agcgcatcaa ataatcctta tcttgatgaa ttaattggat
    18601 atataaaaca aaaagaagtg cagtacagaa gccgattttt gtttccatat aaagatggct
    18661 ataaaacaat taatgtatct gatatagatt tgatttattc tgaagacaag aatactcatt
    18721 taattttaca aaatggatct gtacaaatag ctaatcagac tttagaggag atggaagagc
    18781 aattagatcc tgctgttttt tttcgggcaa acaggcaata cattattaat ataaacagta
    18841 ttgaaagtat ccacaatcac tttaatggca aactaaaagt tgttgtgaga aaacttccac
    18901 aacacgatat attaatcagt aaagaaaaag cacctttatt taaacagtgg ctcgatagct
    18961 gagcaacttt attgctgaaa aactcgtaaa tttttctctt agcggcctta gcgtgttttt
    19021 ttctttgtgt tttagcgaga gacttttttt atttcacgca aagtacgcaa aggagtattt
    19081 gtagcaagtg tagttttttt ttcacgcaaa gtaggtaaag ttgttttcct tagcggtctt
    19141 agcgtgtttt atttctttgc gtcttagcga gagacttttt ttatttcacg caaattaggc
    19201 aaagttgttt ttcttagtgg ccttagcgtg ttttatttct ttgtgccttg gcgagaaact
    19261 tttttttcat gcaaagaacg caaaggagta tttgtagcaa gtgtagtttt tttttcacgc
    19321 aaagatcgca aagggcgaat tctcttagcg tgttcagttt ctttatgtct tagcgagaga
    19381 atttctttta atgcaaagaa taccttagct actcatatta tactaccttt ttgagttaac
    19441 agtagcattt taataagttg tcaataaaca tctatgaggc ataacaccac tctttatttt
    19501 tgtaagacaa aagaaaatta tgttgaaacg atttgtttgt ataacaatat cactattggc
    19561 ttttattatt gtgaacgcac aggataataa gactgaaaag aaagacagta ttgaacatcg
    19621 tatattactc attccctacg acccacgatt ttatttgtca gatgctgata aagacattgc
    19681 agaagaaacc cgaaaagacc ctaaaacaat ccgtaattat tttcacaatc gcagcgaatg
    19741 gtttacctcg cgcgaactaa aaagacatta tcctactatt tcacttttac aaaacgatac
    19801 ggtgcctgcc tatattcagt cagcaggtga tatattcagc caaacagcat ttgactatgc
    19861 tacacctgtg acacgatcac ccgaaagtat gatgaatgca acatcagata aaacactgac
    19921 cgattcgcgt acagcaacca attatctgaa tacagatgta cacaaccgct acatgaaagc
    19981 cgatattcag aataaaaaag tattagccga tttgagcgag cgatttaata cagatatttt
    20041 tgtttttctt actcaatttg agattaaaac caattataaa aactgcttag atattgccaa
    20101 taaaatatat gagcgagaaa tcagacttca ttttaccatc tatgataaaa ccggaaagct
    20161 aatcaatgga aactatgcca ttgcagttat tccatcaaac gtaaatcaga tggacgaaat
    20221 tattttaaag tgttttcctc tgttggcaaa ggggattgca gcatcattat gaggtaaaaa
    20281 tttaattatt tgttacccgg atgctatctt ttcctacctt tagtttacat tcgcaacttc
    20341 aatttattca aacatgagaa aacttttaac actaagcgca ttatttgtaa ttctgtcagg
    20401 gacacaatcg ttggcaagta gtttttggac tgctaagtca gaaactcaag aaatgttatc
    20461 aggcaaacgt cagattgttc ctgataaata caaggtttat actcttgatg tacaagggct
    20521 gaaaaaccaa ctgctcaatg cgccaatgga atttaccatg gcagcaaaac aatcaccggt
    20581 tgtgattaca ttacctatgc ccgatggtag ttggaatagt ttttctatgg tagagtcgcc
    20641 tgttatgcaa cccaaactgg cagctaaata tccatttatt aaaacatact caggcagcgg
    20701 tataaacaat caaccttcat gggttcgtat agattttaca caatggggat ttcatgcatt
    20761 gataagaaca gcggagaacg atatttatat tgacccttat tccatgcaaa ctacctctga
    20821 ttatttagtt tacttcagaa aagattttac aaagtcgcag agcagtatgg tttgtgagtt
    20881 tgacgactta caacaaggtc aaagtgcaaa taagacctta acaaactcaa tgccttcagt
    20941 gaatcgttct ataggtacaa atctgcgtac ctatcgtttg gcattagcct gcaccggtga
    21001 gtatgctgca tactatggtg gaacaaaagc aggagcgttg tcagggatgg ttacatctgt
    21061 aaatcgtgtg acaggtgttt atgaatcgga agtagatgta agacttaatt taattgctaa
    21121 cactgatact ttaatttttc ttaatgccaa caccgaccct tataccaata acagtggcgg
    21181 aaccatgctg ggagaaaatc aatcagtcgt aaactttttt attggtactc ccaattacga
    21241 tattggtcac gtttttagta ccggtggtgg aggtattgcc tacttaggtt gtatctgtaa
    21301 aactgcagat aaggcaaaag gtgtaactgg atctgctgca ccaatcaatg acccgtttga
    21361 tattgattat gtagctcatg aaatggggca tcagtttgga ggcaatcaca cttttaacag
    21421 cgtaacagga agttgtcagg gtaaccgcga agccggtgca gcttatgagc ccggtagtgg
    21481 tgtaaccatt atgggttatg caggaatttg tggctctgat gatttggctg cacacagtat
    21541 agcttatttt catacaaaaa gttttgatga aatagttgat tattcaacct tgtcaactgg
    21601 caactcttgt cctgtaaata ccatcacgtt taacaatgca cctactgtta gtggcggagg
    21661 catatttaat gttcctgtag gcacagcatt taaattaaat ggctatggca gcgaccctga
    21721 tggtgattct ataactttca gttgggagca gtttgactta ggccctgcgg gagcatggag
    21781 tttgccaaca ggtaatgcac ctatgtatcg cagttatgct ccttcaactt ctgcacttcg
    21841 tctttttcca aaattgtctg tattgctcaa tcaggtaaat gctaaaggtg agttgataac
    21901 atcttatgca cgctcattac atttcagact tactgcaaga gataacaaac ccaatggtgg
    21961 tggcgtaact tataacgata cagttacaac agtaaatgtt gttcagactt ctgcaccttt
    22021 tgcaattact tatccaaatg taacgggtat atcatggccc gcattaagta cacaaacaat
    22081 tacatgggaa gtgggtggca ccgatgctgc accatttaac gaacagtttg taaacattta
    22141 cctttcgata aataacggaa caacattccc cataacaatt gcaactaatg ttgctaacaa
    22201 tggcagctat acttttactg ttccaaataa cccatcaaat atttgcagaa tgtgggttga
    22261 aggtgccagc acaggttcta tattttttga tattaacaac aaagtattta ccattacagc
    22321 acctgttgga attaatgaag tcaatagcct tagtcagctt actacctatc caaatccagc
    22381 agacaatgag ctgttggttt atttaggcaa caattatcat ggtcagctaa acataagtct
    22441 ttatagtgtt aacggacagc ttgtgttgtc acaaactata gcagctaaaa atgctacagc
    22501 acaggtagct ttgaatgtag agcaactccc cgcaggttta tattttctga aagcagaaac
    22561 agaaaacggg acagcagagc gtaaagtgat gataaaataa tatcccgata attcatgaaa
    22621 gggttgttga ataaacagcc ctttttttat gttttaattt tcggtttaaa taaagttcaa
    22681 attaactact ttcgcatatc acataaaaca aaaaaggtga agccattttt caaagccgta
    22741 ttcacgctct tatttacttt tataattcta cattctgctg atgcacagtt ttatcagggc
    22801 tcgcagatgt cgtttggcaa aaacagagta caatacacag atttttactg gacatttttc
    22861 agatttaaaa attacgatgt ttataattac gtaggcggaa aagaattagc cacctatgtt
    22921 gctgaacaag cacagaatga aatggatgtt atggaacgca ctttggatta tcggagttcg
    22981 tcacgatttc agattgtaat atttaataaa ctcagtgacc tcaaacaaac caatattggc
    23041 cttgaattag atgatcaggc ttacaatatt ggaggaatga caaaagtaat aggcaacaaa
    23101 gtgcttattt attttgatgg caatcatcag catttacaag aacagattcg tgccggcatg
    23161 gcaagagcct tacttgaaca attgttttat ggaggaaaca tcagagaacg attacaagct
    23221 tctattcttc ttaatcttcc acagtggtac acacaaggct tggtaaccta tctttcaaaa
    23281 ggatggacac ctgaagatga taacaagctg cgtgattaca tcatgacaga aaaaaaaccg
    23341 gtcttaaata aactcattga gcgtgatgaa gtttttgcag gtcagtcgct ttggcattac
    23401 attattacaa cttatggaga aacttcatta agtaatttgg tttatatgac acgcattaac
    23461 cgcagtctcg aaagcggttt tgtttttgtg cttggtgtac gtttaaaaca actttctgcc
    23521 gactggttaa gttatcacac aaaaaaatat caggagtttg aaaagaataa atttccaatt
    23581 gcagcaacac cattaaaagt taaagaaaag aataaaataa tttatcaggt aaagacagat
    23641 ccttttggaa acagagtaag ttatgttact aacaaacgag gccgctataa agttaaaatt
    23701 acggatgtaa aaaccggtaa acgtacttgt gctgataaag gcggatatgt ttcacttact
    23761 acggaaaacg accgatcatt tcccattaca gcatggcatc cgtcaggtaa aatattagct
    23821 atcgttaaag agcaaaaagg aaatattatt ctgaatttgt ttaacacaga aacaaagaag
    23881 tctgactttt cagagataat gagcttttca aaaattctca acatgaccta ttctcctgat
    23941 gggtcaaaac tattgatgtc tgcagtgcag aaagggcaaa gcgatattta tgtatttaat
    24001 gtgcgcggac gaaatttcga tcagattaca aacgatgtgt atgatgactt atatccacaa
    24061 tatgatatta caggcaacaa aataattttt tcttcaaaca gaagcaatga taccttaggt
    24121 gttgatgtta agcaaaatac gccacagaac aattttgatc tttttatggc agacctctac
    24181 aatagagaag gaaatttatt gcgcataacc cattcaccgg agaatgaaac cttccctgtt
    24241 atgagtgatt cgcttcggat ttcttatctg tcagataaaa acggtgtcat caatagattt
    24301 gtatcggaga tggatagcac cataagtttt attgatacaa cagagcatta cagatatttt
    24361 ttcagacatt atgctttaac caatagcaac agaaatattc ttgaacagga tttttcggca
    24421 agaaaaaata agtttacaga actagcagtg aacaatggcc gttacagaat ttacttgaaa
    24481 gacaatatgc cttttgataa atcaaagaca tttgatttga gttatatagc acaacctgtt
    24541 tttcaaccga aacaagagaa taaaccaaag gttgctgaaa cacccactcg tgttgttact
    24601 attgagccat cgcagaagaa agacagcgct gctatagatg taggcaatta tgtttttcaa
    24661 agtgaattca aatcaaagaa gaagaaagaa gaaccaatag aaaataaatc tgttgctaca
    24721 gatactacca aaattgtgca ggaacaagta aagttgcaac ctgatagtaa tgatgtaaaa
    24781 tctactcccg actctgcaat tttccgaatg ccaaagcaac gtaactacga tatttcattt
    24841 tcatcagact atattctcac acagctcgac aacagtttat tcaacactac ctatcagcct
    24901 tacacaggtg gtgctgttta ttttgatccg ggtatgaatg cattgttcaa aatcggtatg
    24961 agcgatttga tgaacgatta caaaatcagt ggagcgtttc gcattggctt cgatttcaac
    25021 agcaatgaat atatgctgat gattgaatct ttgaaaaaaa gaattgataa gcagatactt
    25081 ttttcacgtc aatctaaatt attttctgtt tcagaccttg atgtgttacg tacacatacc
    25141 tataatttaa aatattcagg taagtatcct ttcagcgaaa tttctgcagt taaaggaacg
    25201 attgatgcac gaatggataa gactatttat gttgcgacag agaaggcaca attagagaaa
    25261 agcgatataa caaattattg ggcaagcatt aaaggagaat atatttttga taacactatc
    25321 aataaagcat tgaatcttta taatggtaca cgattcaaaa tttttgctga agtgtttcgt
    25381 aaaatcaata agcccgaaaa aatggtttac atcattggtg cagattttcg tcactatatt
    25441 aaagtacaca gacaaattat ctgggccaac cgtttatcgg caagtacatc attaggtaag
    25501 caaaaattaa tttattattt gggttcacag gataatgtta ttgttccggg aaataatttt
    25561 aataatgaaa ttagtgttga caaaaacgaa gaatatgttt ttcagacatt agcttcgcct
    25621 atgcgtggtt ttaaacagaa tatccgaaat ggtagcagtt ttgcattgtt caattctgaa
    25681 ttgcgtattc cggtatttca atatttgctc aacagaccaa ttcgttctga tttgattcgt
    25741 aattttcagg cagtagtatt tttcgatgcg ggtactgcat ggtcgggcac atcaccattt
    25801 tctgataaga acggattgaa tgaagaagta attgggggcg gaggcagcaa tcccgtaacc
    25861 gttactgtga ttaaacaaat tcaacctgta gttggtgctt atggctttgg atttcgtagt
    25921 agaatattcg gttattttat tcgtgccgac tggggttttg gaattgacga tcgtcaggtg
    25981 cagaaaccac tatttaatct tgcacttgga ctcgatttct gattatcgca aaacaaatgt
    26041 acgatagctg tctaatttta tcagaataaa taagaggatt gtaaatcccc acagtgatga
    26101 gccaccgtaa ctaaaaaagg gtagaggtat tcctattacc ggcatcaggc cgattgtcat
    26161 tccaacattt accataaagt gaaagaatat tactcctgcc acaccatagc catagattct
    26221 tgcaaaggcc gacttctgcc gttctgcaat aaaaataatt ctatataaca atgccagaaa
    26281 caatagtata acagttgttg taccaagaaa cccccattct tcgccaacgg tacaaaaaat
    26341 aaaatcagta ctttgttcag ggacaaaatt gtatttggtt tgtgtgcctt ttaagaaacc
    26401 tttgcctgtt aaaccacctg aaccaatagc aattttactt tgatttacat tgtaacctgc
    26461 acctttaagg tcttcttcca ttcctaaaaa tacctgaata cgttttttct gatggggttc
    26521 taaaacatga ttaaacacat gatcaacact taccacaaca ccaatacaaa tagcaaccaa
    26581 tcctgcaaca acaaaaaatg ttttccattt acgaaatagc aaaacaatta gtgcagcaag
    26641 taccaacaaa gaggtgacga gtatcatttt tggtactaac aatgaaacca caaacaatac
    26701 agcagcagaa ataccaatca tcagcaacca tccgggaata atttcttcgc gataaagaac
    26761 aatacaaaat gacagaaaca ccaatgccac accggtttca ttttctaata gaatgagcag
    26821 aagtggaata aaaaaaattg caactgcata aatacgcgtt cgccaggtgt ttttctttaa
    26881 ccccatagga cttaaaaatt ttgcaatcat catggccgta gcaaattttg caaactcagc
    26941 aggttgaatt ccaaagtcac caaaacgaaa ccacgactta ctacctgcaa cagtatgacc
    27001 gaaaaataat acagcaatca tagagattat acttattgca taaatgacac ccgcaaatgc
    27061 atcataaaac tttccatcaa taatcagaat aaaaaatgct ataacaagtg atgtaagtat
    27121 ccacatcatt tgtttgccat agttttgcga aaagtcagtt attacactat gttcttcatt
    27181 ataaactgca gcaaaaatgt ttagccaacc ggcaataacc ataactaaat atagcagtac
    27241 cagcaaccag tcaatattct gaaaaacact tttctgcttc ctcatttttt agtcatctaa
    27301 aacagttgaa tcatttgcca ttggaactat cactgcatct aacattcgtt tttcaagatc
    27361 aggacgtttt attgtcctca gtaaatattt ctgtaccatc aaacttgcaa taggtgcagc
    27421 aaagtgtgca ccctggccgg cattttcaac taaaacggca actgcaattt ttggattttc
    27481 gcgtggagca aaacaaaaaa acacagagtg gttttttcca tgcggatttt gtgcagttcc
    27541 tgttttgcca cacatcacca aactgtcaac acgagaacca cgggcagtgc cacgctcaaa
    27601 aacccattcc ataccatctt gtatggcatc aaaatatttt ggatcaacca acgtatagtt
    27661 tttcttttta aaaagttcgg gcaggtaatg cttatatccg atttctttta caatatgagg
    27721 cacataataa tatcctttat tggctacaat acaagccgca ttagccatct gtaaaggggt
    27781 aattcctaat tcaccttgtc ctattgctaa tgaaattatt gttgatgatt tccatcgtcc
    27841 ttttccaaag aaacgattgt agtaatcaac tgttggaacg ttgcctcgca attcatgcgg
    27901 catatctaca tcaatttttt taccaatacc aaagcttaga acataatttc gccatgcatc
    27961 aaaagcatct tctgttctgt tgtattttct attgtcaatc acccctcgga aagtctggca
    28021 gtaataagag ttgcacgata ctgcaactgc agtacgtaaa tcaagaggag agctgtgcgg
    28081 atgacaatga acaaaaatat ttccaaccct ataaccacct gcacaactga aatatgtttc
    28141 aggtgtgatg accttctctt gcaaaccaat gagtgagttg ataattttaa atgttgaacc
    28201 gggaggataa taggccatca tggcacgatt aaaaagtggc ttatcctcat cttttaataa
    28261 acgaccatag ttgcgcgaac gctctctacc taccatcagg ttaggattgt aggtagggct
    28321 gtttaccata gcaagtattt cacctgttga agggtcaatg gcaacggcac ttcctctttt
    28381 attctgaaac aatagctctg cgtactcttg taattctgca tctaaggaaa gttttaaatt
    28441 ttcaccggat atggcaagtg tatcatattt cccttcttta aaacgtcctt tctcacgatt
    28501 aaaattatct accatcatat atcgtgtacc
//
LOCUS       DAOOOL010000014        27446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_132_length_27446_cov_106.280457, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000014 DAOOOL010000000
VERSION     DAOOOL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_27446_cov_106.280457"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..480)
                     /locus_tag="PKU82_02360"
     CDS             complement(79..480)
                     /locus_tag="PKU82_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ81747.1"
                     /translation="MTRIESDEWVIDKPANEVFEFLSNCNNLQQLMPEQVTNWQSTTD
                     DCSFTIAGMASLGMEIKERNPGQNIRVERKGKAPFDFVMNYEMQTVDAGKTKLKIAFD
                     ADLNPMLKLMAEKPLRNFLNTLAGNYKNLNQ"
     gene            complement(488..1123)
                     /gene="pyrE"
                     /locus_tag="PKU82_02365"
     CDS             complement(488..1123)
                     /gene="pyrE"
                     /locus_tag="PKU82_02365"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOZ81748.1"
                     /translation="MKDDTASNIADFLLQIKAIKLQPDEPFTWASGWKSPIYCDNRVA
                     LSNPRIRTYIRQELVKAIERNYGRPDAIAAVATGAIAQGALVSESMGLPFIYVRPEPK
                     QHGAKNQIEGDISQIHSVVVVEDLISTGKSSLNAVAALRNSGIIVKGMVSIFNYGFDV
                     AKESFKNAQCPLYSLSNYDAMIIKALQSKYINESQLESLKDWREHPDSWNK"
     gene            1168..1758
                     /locus_tag="PKU82_02370"
     CDS             1168..1758
                     /locus_tag="PKU82_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ81749.1"
                     /translation="MIKIFAGNKTFHFTNNADGLSEDVIKFENSIHKDELKKIFSDSK
                     SIEIVCLCHNAESGFKEFTKMFRNIDAAGGVVYNHEKKLLLIHRLGKWDLPKGKMEDG
                     ESPETSAMREVCEETGVCNLEIIKPLRDTFHIYQQNGEYILKRTYWFEMITSSATNLL
                     PQTEENITEVKWMTKEEVNAAMQFSYESIRYLLSQT"
     gene            complement(1828..3060)
                     /locus_tag="PKU82_02375"
     CDS             complement(1828..3060)
                     /locus_tag="PKU82_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOZ81750.1"
                     /translation="MPSISEKGKHMPPSPIRKLVPFADKAKAEGKKIYHLNIGQPDIE
                     SPKVMLDAIRSIDLKVIEYSHSAGIESYRRKLISYYKKYNININFEDILITTGGSEAI
                     EIAMMTCLNEGDEIIIPEPFYANYNGFSTQAGVTVVPVFSSIRNGFALPSHDEFEKVI
                     TPRTKAIMICNPSNPTGHLYSKEELQSLRDLALKHDLYLFADEVYREFCYDNNSYFSI
                     MHLEGLDENIILLDSISKRYSACGARIGAMISKNKKVMSTAMKFAQARLSPPTFGQIA
                     AEAAIDTPQEYFDNVKAEYVARRNFVVDKLNSMPGVFCAKPSGAFYCMAELPIDDSDA
                     FCQWLLEKFSYQGQTVMLAPATGFYSHAELGKKEVRIAYVLNLDDLKNAMICLNEALK
                     VYPGRVESKSNSVTINQG"
     gene            complement(3149..3598)
                     /locus_tag="PKU82_02380"
     CDS             complement(3149..3598)
                     /locus_tag="PKU82_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ81751.1"
                     /translation="MKKTILLSALAIGMATATFAQEKAAAVQVAPAENKNQADFKFET
                     LEYNYGTIKQGDKVNYTFNFVNTGKEPLIITNAQGSCGCTVPEWPKEPIKKGEAGKIH
                     VTFNSAGKMGLQDKTITITSNAKTSPVVLHIKGNIEAAAATQEAPKN"
     gene            complement(3649..4137)
                     /locus_tag="PKU82_02385"
     CDS             complement(3649..4137)
                     /locus_tag="PKU82_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ81752.1"
                     /translation="MKKLFFAALILCATTAVNAQDDTKKAPAANPNAPDISFESETHD
                     FGTIPYSGNGTYEFKFTNTGKEPLVISNAKGSCGCTVPSWPKEPILKGHSGVISVHYD
                     TKRPGPFTKTVTVSSNGKTAEKVLTIKGNVETKEQTESGTPVKKDNTMTPKETTTSPF
                     GN"
     gene            complement(4240..5457)
                     /locus_tag="PKU82_02390"
     CDS             complement(4240..5457)
                     /locus_tag="PKU82_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOZ81753.1"
                     /translation="MKVSLTARIFIGMLLAILLGHWYNISHTAAELDVFASRISILSD
                     IFLRLIKMIIAPLVFATLVVGVAKVGDFKSVGRIGLKTILYFQFATILALLLGLLLVN
                     FFEPGKIMNLQIPAQSASTGVSAKGLNAKDFITHVIPKSIVEAMANNDILPIVVFALF
                     FGLAAAAVGEKAKMVVNAMDAVSHIMFKVTNFVMKFAPYGVFGAVAAVVAKQGLSVIS
                     GYLYLIATFYGGLLFFGLIVLGSICYFLKIPFIKLLQHIREPIMLAFSTASSESAFPK
                     TIEALEKYGCSNRIISFVLPLGYSFNLDGSIMYMAFATQFIAQAYGMDLNIQQQIMML
                     LMLLVTSKGMAGVPRASMVVIAGMLESFNIPEAGLLLILGVDQILDMGRSATNVVGNA
                     VATAVVSKWEKEL"
     gene            5615..8101
                     /locus_tag="PKU82_02395"
     CDS             5615..8101
                     /locus_tag="PKU82_02395"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOZ81754.1"
                     /translation="MLHKVINVLILFVLFSGVANASDSLKHYKATRIFEPIKIDGQLT
                     ESAWTNTISFSDFVMNRPIEGSAPTQKTEVRIVYDNTAIYVGAMLYDTAPDSILKELG
                     LRDGADPSSNSGFTDINADYFRFVFDPYNTRQDAYDFGVYASGIQADSRYSDYLFDAV
                     WESSVKITDKGWCVEIKIPYSAIRFPSKPIQEWAFQITRNIRRNREFQQWSLTPSTAS
                     NAQNYWGVLDGIENIKPPLRLSLTPYISVAYEHEPVSGSKANQTYSYRGGADIKYGLD
                     ERFTLDMTLLPDFGQVQSDNKRKTLSYEEINYDENRPFFKEGTDIFSRDDLFYSRRIG
                     QTPSGYFDVMAKYDESDIKSNPTESKLLNATKLSGRTNDGIGLGFFNAITNNTYATIK
                     DKNTGLTEKILTEPLANYNVLVADKQWKSNSSVYLINTNVTRTKKFSDSNVTGGGFKL
                     VNKKNTYSLSGGLDYSINFITDPEGINSDKKTDGLRHYLAIKKTSGTIRWGLSQVGVN
                     PTYDPTDFGYYVTPNRINSNANITFFRFKPYGSVQEGNWRFDLNYSQHYKTGYMREMS
                     LQTNLFILFKSYNAIFGGGGFTPAAGREYDPRLNGRYVNALKYWFAYIGVSTDYRRAL
                     ALDMEINISNFIKTYLSEGGSADATLRYRINDKLTVNASSEYNFDPYNFGFTSETDPN
                     IYLFGLRRTNTYINSLAARYIFKNDLSLSIVARHYWFNYAYRKYFLLQDDGGLAVTTD
                     IYNNEFDGSYNFFNADLLFSWRFAPGSTLTFSYKNIFDNGTDGKLETASFTKNLDYVF
                     KNPHVQTFSIKVLYYLDYLQLRKNNISSEN"
     gene            8153..10576
                     /locus_tag="PKU82_02400"
     CDS             8153..10576
                     /locus_tag="PKU82_02400"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81755.1"
                     /translation="MKFILIILAGITLLTKPVMGQTIQAKITDNNNEPLTGAIAELRL
                     TKDSTLNKVAVADATGKVIFDQIKPGSYFIKTTFLGFNNYFSPAFEFTEGENKDFRTI
                     KLSSSSTNLKQATITAFKPLVEVKADKTVFNVENSINSTGSTALELLQKAPGVVVDNN
                     DNIILKGRGGVMVQIDGREIRMTESELADYLRSIQSTDVESIEIISNPSSKYDAQGTA
                     GIINIKLKKNKNYGTNGSITAGYAVGTYSKYNTSLSVNNRTKKFNIYSTYSNNWGDRK
                     NEFYLYREQNPFTFNQSSKSKRGGLSHNYKAGADYQMNAKNSFGIMINGNYSDITGHQ
                     RNRNYISNFYSGVTDSILISNQRYNSINNNFYANLNHHYKDTTGTELTTDFDYGYYNG
                     TRNTYQPNEYTLPDGITPLSATYYKSNTPTTINIFTLKTDYSQSFFKGKLGAGYKVAL
                     VNTVNTFNFYNIEGGIATLDSSRSNKFDYTENVNAIYLNYQRTFNMIDVQVGVRMENT
                     NSNGKLKTSATANDKDVKRSYTDFFPSAGITYNINKDNSAGLIYSSRIDRPNYQELNP
                     FEYKLDELSFRKGNPFLNPQYSNKIELTHTYKYIITTSAGYSHTKDFFAQITDTLGEG
                     KSYLMPRNLATEDVLSISLSASLQPFKWYSIYLNASVDNQKYKADYGGNKTINAQFTS
                     FNLYGQNTFKLPYQFTFELSGWYNSGGVWGGSYKTKAQGSLDVGLQKKLLKDQATLKI
                     SYTDLLHTAPWDSYNTYAGIVSRAWGNWESQQLRVSFTWRFGNKQMKNIRQRSSGSEQ
                     EQKRIGGGD"
     gene            10698..13316
                     /locus_tag="PKU82_02405"
     CDS             10698..13316
                     /locus_tag="PKU82_02405"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOZ81756.1"
                     /translation="MSELSKIYNPQETESKWYAHWMKEKFFKSVPDERPPYTIVIPPP
                     NVTGVLHMGHMLNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVQMLRDRGIK
                     KSDLTRDEFLKYAWEWKEKYGGIILEQLKKLGCSCDWDRTRFTMEDDLSEAVIDVFID
                     LYNKGYIYRGLRMVNWDPAGLTAVSDEEVIHKDVNSKLYYVRYKIDGTADEWITIATV
                     RPETILGDTAVCVHPEDERYAHLKGKFCIIPIVNRRVPIIFDDYIDKEFGTGALKVTP
                     AHDINDYNLGLKHKLEVIDTLTPDGKMSEAAGHYIGEDRFVVRKKITKDLEASGHLVK
                     TEDYKNKVGYSERTDAVIEPRLSLQWFVNMPEISKPALEAVVNGNIKLIPDKFISTYR
                     YWMENVKDWCISRQLWWGQRIPAWYDESGNFVVAKTSQEALAKFAEKNIKTNASAIKQ
                     DDDVLDTWFSSWLWPISVFDGFKDPENKDIKYYYPTNDLVTAPEILFFWVARMIIAGL
                     EYRNDIPFERVYLTGIVRDKLGRKMSKSLGNSPDPLELIEKYGADGVRVGMLLSSPAG
                     NDLLFDESYCEQGRNFANKMWNAFRLIKNWQPDTSLRQSETGRIATAWMKSKLNEQLA
                     LINDSYDKMRISEVLMIKYKLFWDDFCAWYLEMIKPAYGSVAVDTETYNTTIDIFENL
                     LQILHPFMPFLTEELWHELKNRNNRDCIIVSTWPKQSSPDNAILEGMTHAQNIISTLR
                     TFRNEKGISPKTQLQLLTKDKHQTTFDDIILKLANVSSIDKAESFDKGFAFMAGTQEY
                     AIPFDQHIDTAAERLRIEKEIAYNKGFLDTVMKKLGNANFVSKAKPEVIASEEKKKSD
                     AEQKIKSLEQQLNMMS"
     gene            13323..13538
                     /locus_tag="PKU82_02410"
     CDS             13323..13538
                     /locus_tag="PKU82_02410"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOZ81757.1"
                     /translation="MDKPAEWVKVYTTTQPHKVEIVRAVLEEHDIESMVINKNDSSYP
                     GLIGNIEIYVLDKLEVLARFIIKDNEL"
     gene            13535..14359
                     /locus_tag="PKU82_02415"
     CDS             13535..14359
                     /locus_tag="PKU82_02415"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOZ81758.1"
                     /translation="MNNFWTRAFTGAVFVAVMIAAIEFSPYTFMILFMVINLLSIIEF
                     YKLFIRNELTPRIFSGIVLSAILYATSALVASGTASAQLLFINIPMLFLIFIFELYLK
                     SEKPFENIAYTLLGVAYLTLPLALFNSMAFLPGENTGYHSGIIIGYFLILWASDTGAY
                     LSGMKFGKHKLFERHSPKKTWEGSIGGCLAALLLGYTNSLFFTQLALSEWLIVGLLIV
                     ITGTFGDLIESMLKRSLKIKDSGNILPGHGGMLDRFDGLFISIPFVFTYLALIGKV"
     gene            complement(14415..15614)
                     /locus_tag="PKU82_02420"
     CDS             complement(14415..15614)
                     /locus_tag="PKU82_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81759.1"
                     /translation="MKKISFIIFLACAISMQLQAQVITQLGQWSNNANFAVEYYQDKV
                     ITSTTSGIKFIDVSNPSNPVPTTTLASPNNFPMAITVDGSYAFFGGGMTGYFMIADIS
                     SINSPVQVGITYDINGTAYQIAVKGNYAFMPTSYDTLYAIDISNKSLPIVIDRIGMGN
                     FSSGVAIKGNYAYIGTAGGLKVVDISNPSNLNVLSNMNGVYSEVLADLPNNRLFVAKP
                     NQGFDVVDITNPASPSILFQGSGGNSGGDLVYKNGFVFQVGSADVSAFQIGVSSATYL
                     TSFNSTVSGQVNAICAKDSVFYLSTVNNLHVLKLSYLTGMEDNSPLETAIFYLNPAEG
                     VISLKFTSSPPSNIKLYDIMGKLVKQFNSNFSKNLQIGIADLNSGIYFVSFDESYLER
                     RIKFVKR"
     gene            complement(15666..16055)
                     /locus_tag="PKU82_02425"
     CDS             complement(15666..16055)
                     /locus_tag="PKU82_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOZ81760.1"
                     /translation="MAIKEYTLLFFRIGVSLLMLYGHGYPKVQKLFSGTEIQFADPFG
                     LGMKLSFYLVVFAEVICSIFIILGLKTRMAASVLVFNMLVAVFVMATNGEVPELALMY
                     FISYVLILVFGGGKYSIDFNLNSKKKR"
     gene            complement(16271..16342)
                     /locus_tag="PKU82_02430"
     tRNA            complement(16271..16342)
                     /locus_tag="PKU82_02430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16308..16310),aa:Met,seq:cat)
     gene            complement(16399..17643)
                     /locus_tag="PKU82_02435"
     CDS             complement(16399..17643)
                     /locus_tag="PKU82_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOZ81761.1"
                     /translation="MMTNVELINIGDELLIGQIVNTNATWIGQQLSLSGYWLKQITTI
                     GDNEQEIVDALNAAMSRAQIILITGGLGPTKDDLTKDVLCKYFNTQLVFNEQAYSDIE
                     NIFHARGKAVTETNKRQAELPAACIPIYNKKGTAPGMWFNHGEQVFVSMPGVPFEMQA
                     MMQNDVLPKLRQLYESMPLVHKTILTQGIGESDLSDRIAAWENDLPAHLKLAYLPAAG
                     MVRLRLTGKGVEHMEQDMNLRIDKLKELIAPYIFGYDDDTIQSVVGKLLTEKQKTLSV
                     AESCTGGFLAHLITSIPGSSTYFMGGTVSYSNAAKMSQLHVSEQSLKEYGAVSEAVAI
                     EMAIGARKTYNTDFALSTTGIAGPGGGSLEKPIGTVWIGVATHDACFAKKFMLGTNRL
                     RIIQVAADTALNMLRKKINEHN"
     gene            17774..18481
                     /locus_tag="PKU82_02440"
     CDS             17774..18481
                     /locus_tag="PKU82_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOZ81762.1"
                     /translation="MKRFLLIALVLLGQQAYAQKKIFKATNTTPSNQGEGLANNDIIS
                     GLREALKVGTNNSTASASKLDGFYKNPMIKIPFPREIRDAESTLRSMGMDKEVDKFVK
                     TLNRAAEDAAKSAAPIFINSIQHITINDGLTILRGGNDAATQFLKTTSNAALIAAFKP
                     IVQNSLNKVKITQYWNPLMSTYNKVPFVKKVNPDLNQYVTDKAIEGLFKLIAAEELKI
                     RKDPAARINDILKSVFG"
     gene            complement(18623..19642)
                     /locus_tag="PKU82_02445"
     CDS             complement(18623..19642)
                     /locus_tag="PKU82_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ81763.1"
                     /translation="MKQILILFVLIVTVAVFTSCSGQSNEGALNPKSSSALLQDKAGN
                     FWFVSEKEGVFRYDGKSFINYTEKDGMSSNFVYCILEDNEGILWFGTADGISCFDGKK
                     FFNIPITTITNSNSYRKTTTNNFGVPFPQSNFVQGFLLDKTGTLWIGTGNGVYRYNGK
                     TYTQFANNDGIVNNTGFQINGIETFLQDKNGNVWFGGRGSEGLFRFDGKIVSHFKLDD
                     KMWVRPLLEDSKGNLWFGSPRHNMIYCYEGKEFLPFGKNEIKDWVFYMTEDREGNLWF
                     SNGLKGGVTFYDGKTNKNLTKNDGLCYETVWCIASDKDGNVWFINRDESLCRYDGKSF
                     MSLSK"
     gene            complement(19727..24934)
                     /locus_tag="PKU82_02450"
     CDS             complement(19727..24934)
                     /locus_tag="PKU82_02450"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOZ81764.1"
                     /translation="MKILNYLCTAFILLLGQSTQAQLTVTSGNPAAAIVDTLVGTGVS
                     VANVTYTGNANSKGTFRCTGGCNLGITRGVFLSTGNTGQMPTSPPSDFHSSDMSGSGD
                     AQLNLIVSPNLTQDAAVLEFDFTPAADSVGFKFVFASEEYNDYVNTAYNDVFAFYISG
                     PGIPGSPKNLALIPGTSIPVSINNVNNGNSAGAATGPCNHCANFVDNVPNGGTANFFD
                     GFTTVISAGIKVMPCQTYHMKFAIADVNDGVFDSGVFLEAKSFSSIGNVGIAANGVQI
                     IQNLDTVYACDGDSVVLTASPAGSYNWNNGQTTQTIVVTQANITPSGVYQYAVLNSSP
                     ACFASSKQVKVIFVTPTATITPLGSTALCPGGSVVLQANPGSSYLWSNGATSDSIVVS
                     AAGTYTVTVTQFGNCYAVSTPVVVTQSAPVAAINGVLTICSGSNTTLTATAGASYLWS
                     TGATTQSVSVGTSNTYTVTVTYSGGCTATASVNVTANTNPVPSITGNNSICQGQSSTI
                     NAGSYAGYLWSTGATAATINVNTAGNYSVTVTDANGCTGTASFNLTVNANPTPTITGV
                     TSFCAGGNSTLNAGGGYTNYLWSGGATTQTINVTAGGNYAVTVTNAAGCTATTNKVIT
                     VNALPVPSVTGTLSFCAGKNTTLTVPGSYTTYQWSNGATTSNITVSTASTFTVTVTDA
                     NGCTGSKSATTTINTNPVPSIIGNSSICQGQSSTINAGSYAGYSWSTGATAATINVNT
                     AGNYSVTVTDANGCTGTASFNLTVNANPTPTITGVTSFCAGGNSTLNAGGGYTNYLWS
                     GGATTQTINVTAGGNYAVTVTNASGCTATTNKVITVNALPVPSVTGTLAFCSGKNTTL
                     TVPGSYASYQWSNGATTSNITVNTASTFTVTVTDANGCTGSKSATTTINTNPVPSITG
                     NSSICQGQSSTINAGSYAGYSWSTGAATATINVNTAGNYSVTVTDANGCTGTASFNLA
                     VNAIPTPTITGVTSFCAGGNSTLNAGGGYTNYLWNGGATTQTINVTAGGNYAVTVTNA
                     AGCTATTNKVITVNALPVPAISGPAGICPGASANLFAGSYASYLWSNGNTTPSITTNV
                     SGNYTVTVTDANGCVGSSNYNLLAFQNPIPVISGTAAICQNQNSVLDAGSFASWQWST
                     GAVSQTISVTQSGTYSVTVTNTDGCVASTSFNVVVNPLPVPIISGTSAFCSGNSSVLT
                     TGGGSFTSYQWNTSATSNAITVTAQGNYVVTVTDANGCTASTNQAVTVWNLPTPVITG
                     NNSICSGTQTTLSAGNYSAYQWSTGATTDSIAVSNGANYIVTVTDVNGCSGTSAPFAL
                     TINQLPTAIISGSTAICIGQSTSLQIQFTGTAPYSYRYSDGTSVSAILNTNNSAAQIL
                     VTPGTTTDYTLTQINDANCTGNVSGVARVVVNPLPQPVISGSAGECDGITATLTTGNF
                     AAYLWSTGATSQSIAVNSSGSYVCTVTSTAGCKASAIHNFEAYPVPVVSFTNDSSLTC
                     ELPIINFTNTSQYQLGSVFEWNIDNTIYNSLNATHQFSGSGSYTVSLTITSPKNCVGE
                     AIRNVNITVFPLPQASFTIAPDQAPLFNSEVAFGDASANAVSWLWDFGDGDTSTLQNV
                     SHFYADPGKYNAKLYVTNVAGCIDSLAREIFITPFYIPNAFTPNGDGRNETFFDPGYA
                     TNVQSLTMNIYNRWGQLVYTTDNLSKTWDGIDSKGNLSPQGVYVYALKVTTYSGKHYD
                     YTGSVSLIR"
     gene            complement(24991..>27446)
                     /gene="polA"
                     /locus_tag="PKU82_02455"
     CDS             complement(24991..>27446)
                     /gene="polA"
                     /locus_tag="PKU82_02455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOZ81765.1"
                     /translation="EADDIIGTVAKQKEEEGYTVFMVTPDKDYAQLVSPNIFIYKPGR
                     LGNDIEILGVKEIQEKWEVENPKQVIDILGMWGDAVDNIPGIPSVGEKTAKKLIKEYG
                     SMENVIANAENIKGKLGENIRSNVEQARISKQLATIILDVPVEVNDDDLLMSLPDKEK
                     LSQLFTELEFRSLGKRILGEDFSVNREIKTATTASVQTSLFDDEIVRSESDVVRGKNI
                     QNTPHNYTLVENNEAIDGLVAVLLKQKEICFDTETSSLDYFSLSIVGLSFSFNSGTGY
                     YIPCPEDFEKAKNICHKFKPILEHVSIIKIGQNIKFDMLVLKRYGVEVRAPLYDTMIA
                     HYLVEPDMKHGMDYLSETYLGYTPVHIEELIGKKGKNQGSMRDVPIDKIAEYASEDAD
                     ITFQLKQVLAPMVEKHEVDGVLKDIELPLIPVLADMEYEGVKIDVDYLNAYSKELEQD
                     MLKYRSAIFEIAGLEFNLDSPRQLGDVLFGHLKLPVDKKTATGQHSTNEEVLSKLALE
                     YPIAEKILEYRELTKLKSTYVDALPQMVNVKTGRVHTTFNQTIASTGRLSSIGPNLQN
                     IPIRTERGQRVRKAFIARDSEHILLSADYSQIELRLAAEISGDENMLEAFRQGLDIHQ
                     ATAARVYNVALADVTKEMRSKAKMVNFGIIYAISAFGLSQRLGIPRKEAAELIENYFI
                     QYPKLRNYMSETLEFARHNGYVKTLMGRRRYLKDINSRNFTVRGFAEREAINSPLQGS
                     AADLVKLAMIQIHEEFKRQNLKTKMTLQVHDELVFDAHQDEVDLIKPIIREKMIHAIK
                     TQVPLEVEIGIGSNWLDAH"
BASE COUNT         8841 a         5294 c         4803 g         8508 t
ORIGIN      
        1 tgctacacat gcattggtgc agtatgctac attttatgat ggcacaatac tgcgtatcac
       61 gaattaattg aaggaatatt actgatttaa gtttttataa ttccctgcaa gtgtattcag
      121 gaaattacga agtggctttt ctgccatcaa ctttaacata ggattcaaat ctgcatcaaa
      181 agcaattttt agttttgttt tgccggcatc aacagtttgc atctcataat tcatcacaaa
      241 atcgaatggt gcttttcctt tacgttcaac tctaatattc tgaccgggat ttctttcttt
      301 gatttccatg ccaagacttg ccattcctgc aattgtaaaa ctacaatcat cagttgtact
      361 ttgccagttt gttacctgtt caggcattaa ttgctgaagg ttgttacaat tacttaaaaa
      421 ttcaaaaact tcatttgcag gtttgtctat tacccactca tcactttcaa ttcttgtcat
      481 tcattgatta tttattccag ctatcaggat gctcacgcca atcctttaat gattctaact
      541 gactttcgtt aatatacttc gactgcaatg cctttataat catcgcatca taattgctta
      601 atgaatacaa tgggcattga gcatttttaa aactctcttt ggcaacatca aaaccataat
      661 taaaaatgga caccattccc ttaacaatga tgccgctgtt tcttaaagct gctacggcat
      721 tcagactact tttacctgta gaaattaaat cttcaacaac aactacagaa tgtatttgcg
      781 aaatatcacc ttcaatttga ttcttagcgc catgctgttt aggttcaggt ctgacataaa
      841 tgaatggcaa tcccatactt tctgaaacca aagctccttg tgctattgca cctgtggcca
      901 ctgctgcaat agcatcaggg cgcccataat tacgttcaat agccttaact aactcctgac
      961 ggatgtacgt tcttattctt ggattagaca aagcaactct gttatcacaa tatattggcg
     1021 acttccaccc cgatgcccat gtaaatggtt catcaggctg aagttttatt gcttttatct
     1081 gcaataaaaa atccgctata tttgaagctg tgtcgtcttt cattcagcac aaacctaata
     1141 gttatttttt aaaataaaaa atcagaggtg attaaaatat ttgcaggcaa taaaacattt
     1201 cattttacaa acaatgctga tggtctttct gaagacgtga taaagtttga aaatagcatt
     1261 cataaagatg aacttaagaa gattttttct gattcaaaaa gcattgaaat tgtttgtttg
     1321 tgccacaatg ccgaatcagg tttcaaggag tttacaaaaa tgtttagaaa cattgatgca
     1381 gccggtggtg ttgtttataa tcacgaaaaa aagctgcttc taatccacag attgggcaaa
     1441 tgggatttgc caaaaggaaa aatggaagat ggagaatcac cggaaacttc agccatgcga
     1501 gaggtatgtg aggagactgg ggtctgtaat ctggaaatta ttaaaccgct acgagacacc
     1561 tttcatatct atcaacaaaa tggagaatat attttaaaaa gaacttattg gtttgaaatg
     1621 attacttcgt ctgcaacaaa tcttttacca caaacagagg aaaacattac ggaagttaaa
     1681 tggatgacta aagaagaagt aaatgcagca atgcaatttt cgtatgaatc tatacgatac
     1741 ttattaagtc aaacttaaca ccaggaaaaa atcttccatt taaaaaactc ttctgtgatt
     1801 gatttaaaca caaaagccac aaatatgtta gccctgattt attgttacac tatttgattt
     1861 tgattctact ctgcctggat aaacttttaa tgcttcattc aaacatatca tagcattttt
     1921 caaatcatcc aaattaagta cataggcaat gcgcacttct ttctttccca attcggcatg
     1981 actataaaaa ccggtagccg gtgccagcat tactgtttgc ccctgatatg aaaacttttc
     2041 gagcaaccac tgacaaaaag catctgaatc atcaataggc aattcggcca tacaataaaa
     2101 tgcaccactt ggtttggcac aaaatacacc cggcatacta ttcaacttat ccacaacaaa
     2161 atttcttctt gccacgtact ccgcctttac attatcaaaa tattcctgag gcgtatctat
     2221 agctgcctct gctgctattt gtccaaatgt aggcgggctt aaacgtgctt gtgcaaattt
     2281 catagctgtg ctcatcactt ttttattctt agaaatcatg gcaccgattc gtgcaccgca
     2341 ggcactatat cgcttactaa tggagtctaa caaaataata ttttcgtcca atccttcaag
     2401 atgcattatt gaaaaatagc tgttgttatc ataacaaaat tctctataaa cttcatcagc
     2461 aaaaagatac aaatcatgtt tcaaagcaag gtcgcgcaaa ctttgcagct cttcttttga
     2521 ataaaggtgg ccagtaggat tactagggtt acaaatcatg atggctttag ttcgaggtgt
     2581 aataactttt tcgaactcat catgtgatgg aagtgcaaat ccgtttctaa tagatgaaaa
     2641 aacaggtacg acagtaactc cggcttgcgt ggaaaaacca ttataattgg cataaaacgg
     2701 ttcaggaata ataatctcat caccttcatt caaacaggtc atcatggcaa tttcaattgc
     2761 ctctgatcca ccggttgtaa ttaaaatatc ttcaaaatta atattgatat tatacttctt
     2821 gtaatacgaa atcaactttc ttcgatatga ttctatccca gcagaatggc tgtactctat
     2881 aactttaagg tctatactcc tgattgcatc gagcattact tttggggatt caatatcagg
     2941 ctgaccaata ttgaggtgat atattttttt tccttctgct ttggctttat cggcaaaagg
     3001 caccaatttt ctgattggtg aaggaggcat gtgtttgcct ttttctgaaa tagatggcat
     3061 gcagttttta tattaattac atacaaaaaa taagggacga ttgaattcaa tcgtccctgc
     3121 aaaattataa ttaatctaat taccttagtt aattctttgg tgcttcttgt gtagcagctg
     3181 cagcctcaat atttccttta atgtgtaaaa caacagggct tgtttttgca ttagaagtaa
     3241 ttgtaatagt tttatcctga agacccatct tacctgctga attgaaagtt acatggattt
     3301 ttccggcttc gcccttttta attggctctt ttggccactc aggtactgta cagccacaag
     3361 atccttgtgc attagtaata attaaaggct cttttcctgt gttcacaaag ttaaaagtgt
     3421 aattcacctt gtcaccttgt ttaatagtac cataattgta ctccaaagtt tcaaacttaa
     3481 agtcagcctg atttttgttt tcagcaggag caacctgaac tgctgctgct ttttcttgtg
     3541 cgaatgtagc agttgccata ccgatagcta atgctgaaag aagaattgtt tttttcattt
     3601 tttgaaattt gattgttgat ttgtgtgttt aatatttatt ttatgtgatt aattaccaaa
     3661 tggacttgtg gtggtctctt ttggagtcat tgtattatct tttttaacag gagttccact
     3721 ttctgtttgc tcctttgttt caacatttcc tttaatagtc aacacttttt ctgctgtttt
     3781 tccattagaa gaaactgtta cagtcttagt aaatggcccc ggacgcttag tatcataatg
     3841 aacactgatg acaccggaat gacctttaag aataggttct tttggccacg atggaactgt
     3901 acatccgcaa cttccttttg cattagaaat tactaatggt tctttacctg tatttgtaaa
     3961 tttaaactca taggtaccat taccactata aggtattgta ccgaaatcat gcgtttcgct
     4021 ttcaaagcta atatcaggtg cattaggatt agcagcaggt gctttttttg tatcatcctg
     4081 tgcatttact gcagttgttg cacataaaat cagtgctgca aaaaataatt tcttcatatt
     4141 ttgatttaat ttatttgtgg tgtaaagtta aattatttta ctctttaacg acaaacaatg
     4201 cgccaaagtt agacttaggc tgtgaaaaaa gtatgaacgt tataactcct tctcccattt
     4261 agacactact gccgttgcca ctgcattgcc tacaacattg gttgccgacc gacccatatc
     4321 caatatctga tcaactccaa gtatcaacaa caatcctgct tcaggaatat taaatgactc
     4381 gagcattccg gcaataacaa ccatactcgc tcttggcaca cctgccattc ctttgctggt
     4441 aacaagtagc atcagcaaca tcataatctg ctgctgaata ttcaaatcca tgccataagc
     4501 ctgagcaata aactgcgtag caaatgccat gtacattata cttccatcaa ggttgaaaga
     4561 atagcctaag ggtaatacaa aactaattat gcgattgctg caaccatatt tttccaatgc
     4621 ctcaatagtt ttaggaaatg ccgattcgga acttgctgtc gagaatgcca acatgatagg
     4681 ttcgcgtata tgttgtaaaa gttttataaa tggtattttc aaaaaataac aaatcgaccc
     4741 caatacaatc aacccaaaga aaagcagacc tccatagaag gttgcaatta aatagagata
     4801 acctgaaata acactgagtc cctgttttgc aacaacagca gctactgcac caaacacacc
     4861 ataaggtgca aacttcatta caaagtttgt aaccttaaac ataatatgcg aaactgcatc
     4921 cattgcatta acaaccattt tggctttctc gccaacagcc gctgctgcta atccaaaaaa
     4981 caaagcaaat actacaattg gtaaaatatc attattggcc attgcctcta caatactttt
     5041 tggaataaca tgggtaataa aatctttagc attgagacct tttgctgaaa ctcctgtaga
     5101 tgctgactgt gcaggtatct gtagattcat aatcttaccg ggttcaaaaa agtttaccaa
     5161 gagtaatccg agaagaagcg caagaatagt tgcaaactga aaatacaata ttgtctttag
     5221 gccaatgcgt cctacgctct taaaatcgcc cactttagca acaccaacta ccagcgttgc
     5281 aaaaactaat ggagcgataa tcattttaat caagcgaaga aaaatatcac tcaatatgct
     5341 aatccttgaa gcaaatacat caagttcggc tgcagtgtgt gagatattat accaatgtcc
     5401 cagcaaaatt gccagcaaca taccaataaa tatccgggct gttaaactaa ctttcacaat
     5461 aaaaacttat gaggcaaatc tactgctctg aattgaaaaa acaagtctgt gatttttaga
     5521 gaataaatta atattaacat ctttaacgct gattaacttt tacttaactc gaaccactgt
     5581 atgtagtgat agttttgccg acaattactc ccgtatgttg cacaaggtca ttaatgtcct
     5641 tattctattt gttctttttt caggtgtagc taatgcctca gatagtctta aacattataa
     5701 agcaacacgc atttttgaac ctataaaaat tgatggtcag ttaacagaat ctgcttggac
     5761 aaacaccatt tcatttagcg attttgtgat gaaccgcccg attgaaggta gtgcaccgac
     5821 acaaaaaaca gaagtgcgta ttgtttatga caacactgca atttatgtag gcgctatgct
     5881 ttatgacaca gcacctgaca gcattttaaa agaattaggc ctacgtgatg gtgcagaccc
     5941 ttccagcaac agtggtttta cagacatcaa tgcagattat ttcagatttg ttttcgaccc
     6001 ctacaataca cggcaagatg cttatgattt tggagtgtat gcctcaggca ttcaagctga
     6061 cagtcgctat agtgattatt tgtttgatgc tgtttgggaa agttctgtaa aaattacaga
     6121 caaaggttgg tgtgtagaga taaaaatccc atactctgca attcgatttc cttcgaaacc
     6181 tattcaggaa tgggcttttc agataacaag aaatataagg cgcaacagag aatttcagca
     6241 atggagtctt acaccatcaa cagcatcaaa tgctcaaaac tattggggtg tactcgatgg
     6301 cattgaaaac attaaacctc cgctcaggct ttcactaaca ccatatatta gtgtagccta
     6361 cgaacatgaa cccgtttccg gttccaaagc aaatcagact tacagctatc gtggtggagc
     6421 agatataaaa tacggactcg atgagcgttt tacattagac atgaccttgc ttcctgattt
     6481 tggacaagta cagtctgaca ataaaagaaa aacactaagc tacgaggaaa taaactatga
     6541 tgaaaaccgt ccatttttta aagagggtac agatattttt tcgagagacg atttattcta
     6601 cagtagaaga attggtcaaa caccatcagg atattttgat gtgatggcga aatacgatga
     6661 aagtgatata aaatcaaatc caacagaatc gaaactacta aatgcaacaa aactttccgg
     6721 acgaaccaat gatggaattg gtcttggttt ttttaatgct ataactaata acacctatgc
     6781 aacaattaaa gacaaaaaca cagggctaac agaaaaaatt ctgactgagc cacttgccaa
     6841 ttataatgtt ttagtagcag ataagcaatg gaaaagcaac tcatcggttt atctgataaa
     6901 taccaatgta actcgaacaa aaaaattcag cgattcaaat gtaacaggag gcggatttaa
     6961 attagtgaat aaaaaaaata cctatagttt aagtggcgga cttgattaca gtattaattt
     7021 tattacagat cctgaaggaa taaattctga taaaaaaact gatgggttac gacattatct
     7081 tgctataaaa aaaaccagtg gaacaattcg ctgggggctt tcgcaggttg gtgttaatcc
     7141 tacttatgac ccaacagatt ttggctacta tgttacacca aacagaatta attccaatgc
     7201 taatattaca tttttccgat tcaagcctta tggttctgta caggaaggta attggcgttt
     7261 tgacctaaac tattcacagc attataaaac aggctacatg agagaaatgt ctttacagac
     7321 aaatcttttt atactattta aatcgtacaa tgcaattttt ggtggtggtg gatttacccc
     7381 tgcagccgga cgtgaatatg atccaagact aaatgggcgt tatgttaatg cgttaaaata
     7441 ctggtttgca tatatcggag ttagtacaga ttacagaagg gcattagcat tagatatgga
     7501 aattaatatc agtaacttta ttaaaaccta tctttccgaa ggcggaagcg cggatgctac
     7561 cttacgttat cgcattaatg acaaactcac agttaatgct tcttcggaat acaattttga
     7621 tccctataat ttcggtttta caagtgaaac tgacccgaat atttaccttt ttgggttaag
     7681 aagaaccaat acctatataa acagtcttgc agcacgatat atatttaaaa atgatctgtc
     7741 gctgtcaatt gttgcacggc attactggtt taattacgca tataggaaat actttttgct
     7801 gcaggatgac ggtgggcttg ctgtcacaac cgatatttac aacaacgaat ttgatggcag
     7861 ctataatttt tttaatgctg accttttgtt ttcatggcga tttgctcccg gcagcacatt
     7921 gactttttcg tacaaaaaca tttttgacaa tggtactgat ggcaaacttg aaactgcatc
     7981 attcactaaa aatcttgatt atgtttttaa aaatccgcat gttcaaacat tttcaattaa
     8041 agtactttat tatctcgatt atttacaatt aagaaaaaat aatatttcat cagaaaacta
     8101 aataatacat agcagcaata ctatattttc ccgcaaccat aacacacaaa caatgaaatt
     8161 tattctaatt atcttggcag gaataaccct gttgacaaaa cctgtaatgg gtcaaactat
     8221 tcaggctaaa atcacagaca ataataacga gccgcttaca ggtgccattg cagaactgcg
     8281 actcactaaa gattcaacct taaacaaagt tgcagtagct gacgctacag gaaaagtaat
     8341 tttcgaccag ataaaacccg gtagctattt tataaagaca acgtttttgg gattcaacaa
     8401 ttattttagt cctgcatttg aatttacaga aggagaaaac aaagattttc gcactataaa
     8461 actttcatca tcatcaacta atcttaagca agcaaccatc actgcattta aacccttggt
     8521 tgaagttaaa gcagataaaa ctgtatttaa tgtagaaaac agtattaact caacaggaag
     8581 tactgcactt gaattgcttc aaaaagcgcc cggtgttgtt gtagataata acgataacat
     8641 tattttaaaa ggacgtggtg gcgtgatggt tcagattgat ggacgtgaga ttagaatgac
     8701 agaaagcgaa cttgctgact accttcgttc tattcaaagt acagatgtgg aaagtattga
     8761 aattatttca aacccatcat ccaaatatga tgcgcaaggc acggccggaa tcatcaatat
     8821 taaactaaag aaaaacaaaa actatggcac caacggaagt ataacagcag gttatgccgt
     8881 tggaacatat tctaaataca acacctcact ttcagtcaac aacagaacaa aaaagtttaa
     8941 tatttactct acttacagta ataattgggg tgacagaaaa aacgagttct atctgtatcg
     9001 cgaacaaaac ccgtttacat tcaatcaatc atcaaaatcg aaacgtggtg gcctaagtca
     9061 taattacaaa gccggtgcag attatcagat gaatgctaaa aatagttttg gcattatgat
     9121 aaatggaaat tacagcgaca taaccggaca tcagagaaac agaaactata tcagcaattt
     9181 ttattcagga gtaacagaca gtatccttat ttccaatcaa cgttataact ctataaacaa
     9241 taatttctat gccaatctaa atcatcatta caaagacaca acaggaacag agttgacaac
     9301 agactttgat tacggctact acaacggaac aagaaacacg tatcaaccaa atgaatacac
     9361 attgcctgat ggaatcacac cactttctgc gacatattac aagtcaaata caccaacaac
     9421 tatcaatatc tttactttaa aaacagatta ttcgcaatcg ttttttaagg gcaagttagg
     9481 agccggatat aaggttgctt tggtaaacac tgtcaacaca tttaattttt acaacattga
     9541 aggtggtatt gcaacattgg attcttcacg tagcaataaa tttgattata cagaaaatgt
     9601 gaatgccatt tatttaaatt accaacgcac attcaatatg attgatgtgc aagtaggtgt
     9661 ccgcatggag aatacgaata gtaatggcaa attaaaaacc tctgccaccg caaatgataa
     9721 agatgtgaaa cgaagctata ctgacttttt ccccagcgca ggcatcacct acaatatcaa
     9781 caaggataat tctgcaggat taatttacag ttcgcgaatt gacagaccaa attatcagga
     9841 gttaaatccg tttgaatata agcttgatga attaagtttc agaaaaggaa atccattttt
     9901 aaacccacaa tattcaaata aaattgaact tacacacact tataaatata tcattacaac
     9961 atcggccgga tatagtcaca caaaagactt ttttgcacaa attactgaca cacttggcga
    10021 aggaaaaagc tatttaatgc ctcgcaatct ggctacagaa gatgtgttga gcattagttt
    10081 aagtgcatca ttacaacctt tcaaatggta cagtatttat ttgaatgcaa gcgttgacaa
    10141 tcaaaaatac aaagccgatt atggaggtaa taaaaccatc aatgcacagt ttacctcatt
    10201 taatttatat ggtcaaaaca catttaagtt accctatcag tttacatttg aactttcggg
    10261 atggtacaac tcaggaggtg tttggggtgg ctcatacaaa acaaaggcac agggatcatt
    10321 agatgtgggc ctacagaaaa aattattgaa agatcaggca acattaaaaa tttcatatac
    10381 cgacttactc catacagctc catgggacag ttacaacact tatgcaggca ttgtgagtcg
    10441 tgcatggggc aattgggaaa gccagcaact cagggtgtcg tttacctggc gatttggcaa
    10501 caaacaaatg aaaaacatca gacaacgaag cagtggtagt gagcaggaac aaaaaagaat
    10561 tggaggaggc gattagcaaa gtcctccttt tacagaaaga aagtacactt atttttcaaa
    10621 gcctgctttt atagcaggct ttgttcgttt tgcacatagc taatttctta tttttgcatt
    10681 cacacttttt aaagcacatg tcagaattat caaaaatata taacccacag gaaacggaat
    10741 ccaaatggta tgcacattgg atgaaagaaa aattttttaa atcggttcct gatgagcgtc
    10801 cgccctacac tattgttatt cctccaccaa atgtaacagg agtgttgcac atgggtcaca
    10861 tgctcaacaa caccatacag gatgtactga tccgcagagc acgcatgtta ggtaaaaatg
    10921 catgctgggt tccgggaaca gatcatgctt ctattgcaac agaagctaag gttgtgcaaa
    10981 tgttgcgcga cagaggaatt aaaaaatctg accttacaag agatgagttc ttaaaatatg
    11041 cctgggagtg gaaagaaaaa tatggcggaa ttattcttga gcaattaaaa aaattaggtt
    11101 gcagttgtga ttgggatcgc acacgattta caatggaaga cgatttaagt gaagcggtaa
    11161 ttgatgtatt tattgacttg tacaacaagg gttacattta tcgtgggctt cgcatggtga
    11221 actgggatcc ggcaggatta actgcagtaa gtgatgaaga ggtaatacat aaagatgtta
    11281 actctaaact ttactatgta agatataaaa ttgatggcac tgctgatgaa tggattacta
    11341 ttgcaacagt tcggcctgaa acaattttag gcgacactgc cgtttgtgta catccggaag
    11401 atgaacgtta cgcacattta aaaggaaaat tctgtattat tccaattgtg aatagaagag
    11461 tacccataat ttttgatgac tatatagaca aggaatttgg taccggtgca cttaaagtaa
    11521 ctcctgcaca tgacatcaac gactataatc ttggattaaa acataagctc gaagtaattg
    11581 acacactcac acccgatggc aagatgagtg aagcagcagg gcattatatt ggagaggaca
    11641 ggtttgttgt aagaaaaaaa attacaaaag accttgaagc aagtggtcat ttagttaaga
    11701 cagaagatta taaaaacaaa gtaggataca gcgaacgtac tgatgcagtt attgaacctc
    11761 gcctgagttt acaatggttt gtgaatatgc cggaaatcag caagcctgca ttagaagcag
    11821 tggttaatgg caacatcaaa cttattcctg ataaatttat cagcacctat cgctactgga
    11881 tggagaatgt taaagactgg tgtatatcac gtcagctatg gtggggacaa cgtattcctg
    11941 catggtatga tgaatcagga aactttgtgg tagccaaaac ttcgcaggag gcattggcga
    12001 agtttgcaga aaaaaacata aaaacaaatg catcggcaat aaaacaggat gatgatgttt
    12061 tagacacatg gttttcttca tggctgtggc caataagtgt ttttgatgga tttaaagatc
    12121 ctgagaataa agacatcaaa tactactacc ctaccaacga tttggtgaca gcaccggaaa
    12181 ttcttttctt ttgggtggca cgaatgatta ttgcgggatt agaatatcga aatgacattc
    12241 cttttgagcg tgtttatctt accggaattg ttcgtgataa gttaggcaga aaaatgagta
    12301 agtcattagg taattcaccc gacccacttg agctaataga aaaatatggt gcagatggtg
    12361 ttcgtgtagg tatgttgctt tcttcacctg ccggtaacga cctattattt gatgaatcat
    12421 actgtgagca agggcgcaat tttgccaaca aaatgtggaa tgcatttcgt ctgataaaaa
    12481 actggcagcc tgacacatca ctgcgtcaat ccgaaacagg aagaattgca acagcatgga
    12541 tgaaatcaaa actcaatgaa caattagcac tcatcaacga cagttatgat aaaatgcgca
    12601 tcagtgaagt gcttatgatt aaatacaagt tgttttggga tgatttctgt gcatggtatt
    12661 tagaaatgat taaacccgct tacggaagcg tagccgttga tacagaaacc tacaatacaa
    12721 ctattgatat ttttgaaaac ctgctacaaa tactgcatcc atttatgcca tttttaacag
    12781 aagagttgtg gcatgaactt aaaaacagaa ataacagaga ttgcattatt gtaagtacat
    12841 ggccaaagca atcatcaccg gataatgcta tattggaagg gatgacacat gcacaaaata
    12901 taatttctac tttaagaact tttcgaaacg aaaaagggat atcaccaaaa actcagttac
    12961 agttattgac aaaagataaa catcaaacaa cgtttgatga catcatactt aaattagcca
    13021 atgtcagtag cattgataaa gcagaaagtt ttgataaagg atttgctttt atggcaggca
    13081 cacaggaata tgctatccct tttgatcagc atattgatac tgctgcagaa cgtctccgaa
    13141 ttgaaaaaga aattgcatac aacaaaggct ttcttgatac ggtaatgaaa aaattaggta
    13201 atgcaaactt tgtaagcaaa gccaagcctg aagtaattgc aagtgaagag aaaaagaaat
    13261 cggatgcaga acaaaaaata aaatcattag agcaacaact taatatgatg agctaactga
    13321 atatggacaa gccggcagaa tgggtcaagg tatatactac aacacaacca cacaaagtag
    13381 aaattgtgcg tgctgtgtta gaagagcatg atattgaaag tatggtaatc aataaaaatg
    13441 attcaagtta tccgggatta attggcaata ttgaaatata tgtactcgat aaactggaag
    13501 tgcttgcacg gtttattata aaagacaacg aactttgaat aacttttgga caagagcatt
    13561 tacgggtgct gtatttgtag cagtgatgat tgctgccatt gaattcagcc cttatacatt
    13621 catgatactt ttcatggtca tcaacctgtt aagcatcatt gagttttaca aactgtttat
    13681 ccgaaacgaa cttacgccaa gaattttttc aggcattgta ctaagtgcga ttctttatgc
    13741 gaccagtgca ttggtagctt cggggacggc atctgcgcag ttattgttta taaacattcc
    13801 tatgcttttt cttattttca tttttgagtt atatctcaaa tctgaaaaac catttgaaaa
    13861 tatagcttat acgttgttgg gtgtagctta tctgacatta cctcttgcac tatttaactc
    13921 tatggcattt ttacccggtg aaaatacagg ctatcatagt ggaattatta ttggctattt
    13981 cttaattcta tgggcaagtg acaccggagc ctatctttcg ggaatgaaat ttggaaagca
    14041 taaacttttc gagcgtcatt ctcctaaaaa gacctgggaa ggcagtattg gcggttgtct
    14101 tgctgcatta cttttaggtt atactaattc tttgtttttt acacagcttg ctttatcaga
    14161 atggctgatt gttggtttgc ttattgtaat tacaggaact tttggcgatt tgattgaaag
    14221 tatgctgaaa cgtagcctta aaatcaaaga ctcaggaaac attcttcccg ggcatggagg
    14281 tatgctcgat cgctttgatg gactatttat ttctattcca tttgttttta cttacctggc
    14341 tttgattggg aaggtataat aagtaacctt taattaactt ctctttttta aataactaag
    14401 atgaaaacct tccattacct ctttacaaac tttattcttc tttcaagata agattcatca
    14461 aaagaaacaa aataaattcc tgaattcaag tcagctatcc cgatttgcaa attttttgaa
    14521 aaattagaat taaattgctt cactaatttt cccattatat catacaactt aatattggat
    14581 ggaggactgg aggtaaattt caatgagatg acaccctcag caggattaag gtaaaaaatt
    14641 gctgtctcta atggactatt atcttccatg cctgtgaggt agcttagctt cagaacatgt
    14701 aaattattaa ctgttgataa ataaaaaact gaatcttttg cacaaattgc atttacctga
    14761 ccactcactg tactattaaa cgaagtcaaa tatgttgcag aggaaactcc tatttgaaat
    14821 gcactaacat cagcagatcc tacttgaaat acaaatccat tcttataaac taaatctcca
    14881 ccggaatttc cacctgaacc ctgaaaaaga attgatggac ttgctggatt agtaatgtca
    14941 acgacatcga aaccctgatt tggttttgcc acaaataatc tgttatttgg caaatcagca
    15001 agaacttcag aataaacacc attcatattt gacaacacat ttaagtttga aggatttgaa
    15061 atgtcaacta cctttaaacc tccagctgtt ccaatatatg cataattacc ttttatagca
    15121 acaccagaag aaaagtttcc cataccaatt ctgtcaataa caataggtaa acttttattg
    15181 ctaatatcaa ttgcatataa agtatcgtaa cttgtaggca taaaagcata attaccttta
    15241 actgctatct gataagctgt accattaata tcataagtta ttcctacctg aaccggtgaa
    15301 ttaattgatg aaatatctgc aatcataaaa tagcctgtca tacctccgcc aaaaaacgca
    15361 taactcccat ccactgtaat tgccattggg aagttattag gagatgctaa ggttgtagta
    15421 ggaactggat tcgatgggtt cgacacatca ataaatttga tgcctgaagt ggtgctggtg
    15481 ataactttat cctgataata ttcaactgca aaattggcat tattactcca ttgaccaagt
    15541 tgggtaatta cctgagcttg taattgcata ctaattgcac aagccaaaaa aataataaaa
    15601 cttatctttt tcatgatttt gaaattttta ttcaacattt aaaaataaat taaaatcaac
    15661 tatgctcacc ttttcttctt tgaattaaga ttaaaatcaa tagagtattt acctccgcca
    15721 aaaactaaaa tcagtacata agagataaaa tacatgagtg ccaattcagg aacttctcca
    15781 ttagtagcca tgacgaaaac tgcaacaagc atattaaaaa ccaatacaga tgcagccata
    15841 cgtgttttta gtccgagtat gataaatata gaacaaatca cttcagcaaa aactaccaga
    15901 taaaaagaaa gcttcatgcc aagcccaaac ggatcggcaa attgaatttc tgttccacta
    15961 aataatttct gtactttagg atagccgtga ccataaagca tcagcaatga aactcctatt
    16021 ctgaaaaaca gcaaagtata ttcctttatg gccattcgat ttttaatgct aattaaatgt
    16081 gtcatgccta tactaatttt gtttctccaa aaataaaatt ttccttggat ttcaatcaat
    16141 accacaaaat cagacacaat cggcaatatt tcaactcaga ttgaagaatg aacttgcgtt
    16201 ttgaaaatct atatatcaat aaagtaaaag gaaataaaaa agctgcactc tgtgcagctt
    16261 aatcttttag tagcgggggc tggattcgaa ccaacgacct ttgggttatg agcccaacga
    16321 gctacccctg ctccaccccg cattgttatt ttgagggtgc aaaagtaatc actttgtcct
    16381 tatctgcaaa tatattcctc aattatgctc atttattttc tttctgagca tgtttaatgc
    16441 agtatccgca gctacctgaa tgattcgcag acggtttgta cccaacataa actttttagc
    16501 aaaacaagca tcgtgtgtag caactccaat ccatacagtg ccaatcggtt tttccaaact
    16561 tcctccacca ggccctgcaa tgcctgttgt actcaatgca aaatctgtat tataagtttt
    16621 tcttgcacct attgccattt ctattgcaac cgcttcactg acggcaccat attccttcaa
    16681 tgattgttcg ctgacatgca actgactcat ttttgctgca ttactgtaac tcactgtacc
    16741 gcccataaag tatgttgaac taccggggat tgaagtaata agatgtgcca aaaatcctcc
    16801 agtacaactt tctgcaactg aaagagtttt ttgtttctca gttaaaagtt ttcctacaac
    16861 agattgaatt gtgtcatcat cataaccaaa aatataaggc gcaattaatt ctttgagttt
    16921 atcaatcctc aggttcatgt cttgctccat gtgttcaaca cctttacctg taagtcgtaa
    16981 acgaaccatt cctgcagcag gcagataggc taattttaaa tgagcaggca agtcgttttc
    17041 ccatgctgca atcctatctg acaaatcgct ctctcctata ccttgcgtaa gaattgtttt
    17101 atgtactaaa ggcattgact catatagctg ccttaattta ggaagtacat cattctgcat
    17161 cattgcctgc atctcgaatg gcacacccgg catgctcaca aaaacctgct cgccatgatt
    17221 gaaccacatt cccggagcag ttcctttctt attgtaaatt ggaatacatg cagcaggcaa
    17281 ttcggcctgc cgtttgtttg tttctgtaac tgccttacct cttgcatgaa aaatattttc
    17341 aatatcacta tatgcctgtt cgttaaacac tagttgtgta ttaaaatatt tacataacac
    17401 atcctttgtt aaatcatctt ttgttggccc tagtccacct gtaatgagaa taatttgtgc
    17461 acgactcatt gcagcattta atgcatcaac aatttcctgc tcattatcac caatggtggt
    17521 aatttgtttc agccaataac cggaaaggct tagttgctgt ccaatccatg ttgcattggt
    17581 atttacaatc tgtccaatga gtagttcgtc accaatattt attagctcta catttgtcat
    17641 cataaagtga actttaaatt aattaagtat tttagctgca ttaatttcag attaagtaaa
    17701 caaacaatat tgattgcaga catataatgg caatatgttt gaattgaaaa acaaaattct
    17761 aaaaaaatag attatgaaac ggtttctttt aattgcgctt gtgttattag gacagcaagc
    17821 ttatgcacag aaaaagattt tcaaagctac aaatactacc ccttcaaatc aaggtgaagg
    17881 tttggctaac aacgacatca tcagtggcct cagagaagca cttaaagtag gcacaaacaa
    17941 ctctactgct tcagcatcaa agcttgatgg cttttataag aacccgatga taaaaatacc
    18001 tttccctcgt gaaatacgcg atgcagaaag cactttacgc agcatgggca tggataaaga
    18061 agttgacaaa tttgttaaaa cattaaaccg tgcagcagaa gatgcagcaa aatcggcagc
    18121 accaattttc atcaattcta ttcagcatat aactattaat gatggactta ccatattaag
    18181 aggtggtaat gatgcagcaa cacaattttt aaaaaccact tcaaatgcag cgctgatagc
    18241 agccttcaag ccaatagtcc aaaattcgct taacaaagta aagataacgc agtactggaa
    18301 tccattgatg tcaacttaca ataaagtgcc atttgtaaaa aaagtaaatc cagacctgaa
    18361 tcaatacgtt actgataaag ctattgaagg tctttttaaa ctcattgctg cagaagagct
    18421 gaaaatcaga aaagatcctg ctgcacgaat caacgatatt cttaaaagtg tttttgggta
    18481 aatcaacggt ttgcctattg acgaagtatt gtaattttac atcaatgcac aagcccgaca
    18541 gtaatcgggc ttgtacattg catagacatt aaactgaagt gtttgttctc catctttact
    18601 taaatgtacc tgcctccaat tgttactttg ataaactcat aaatgatttt ccgtcataac
    18661 gacataaact ttcatcccta tttataaacc aaacattacc gtccttgtct gatgcaatgc
    18721 accaaacggt ttcataacac aaaccgtcat ttttagtaag gtttttattt gttttcccgt
    18781 catagaaagt cacacctccc ttcaaaccat tactaaacca aaggtttcct tctctatctt
    18841 ctgtcatata aaaaacccaa tctttaattt cgttcttgcc aaatggaaga aattcttttc
    18901 cttcatagca gtatatcatg ttatgacgag gagagccaaa ccaaagattt cctttactgt
    18961 cttctaaaag tggtctgacc cacattttgt cgtctagttt gaaatgactt actattttgc
    19021 catcaaaacg aaaaagccct tcactgccgc gaccaccaaa ccaaacatta ccgtttttgt
    19081 cttgcaagaa agtttcaata ccattaattt gaaaaccggt attgtttaca ataccatcat
    19141 tattggcaaa ttgagtgtat gttttgccat tgtaacgata cactccattt ccggttccaa
    19201 tccaaagtgt tcctgtttta tcgagtaaaa atccttgtac aaaattgctc tgcggaaatg
    19261 gtacaccaaa gttgtttgtt gtggttttac ggtatgaatt actattagta atagttgtta
    19321 taggtatatt aaagaacttt ttcccatcga aacaagaaat tccgtctgct gtgccaaacc
    19381 aaagtattcc ttcattatct tctaaaatac aatacacaaa attgctactc attccatctt
    19441 tttcggtata gtttataaaa gatttaccat cataacgaaa aacgccttcc ttttctgaaa
    19501 caaaccaaaa gtttcctgct ttatcttgta gtaatgcact acttgattta ggatttaatg
    19561 ccccttcatt tgattgccca ctgcaagaag taaaaactgc tactgttaca atcaacacaa
    19621 ataatattaa aatctgcttc ataaataaat ataaaactct taacagaggt atcaattggt
    19681 taaacacaac aatttagtta tgtcttctga tgggcgcagg taaaatttaa cggatcaacg
    19741 aaacacttcc tgtataatcg tagtgtttac cactgtaagt tgttacttta agtgcataca
    19801 cataaacacc ttgaggggaa agatttcctt ttgaatcaat gccatcccag gttttactta
    19861 aattatctgt ggtataaacc aactgccccc aacggttata gatattcatt gtcaggcttt
    19921 gtacattggt agcataaccc ggatcaaaaa acgtttcgtt gcgtccatca ccattgggtg
    19981 taaacgcatt tggaatataa aatggggtga tgaaaatttc acgtgctaat gaatctatac
    20041 aaccagcaac attggtgaca taaagttttg cattatactt tcccggatca gcataaaagt
    20101 gacttacatt ttgcaaggta gatgtatcgc catctccaaa atcccacaac cagctaacag
    20161 catttgcaga ggcatctcca aatgctactt cggaattaaa taatggtgcc tgatcgggtg
    20221 ctattgtaaa tgatgcttgt ggcaaaggaa atactgtgat gtttacattt ctgatagctt
    20281 cgcctacaca attctttggc gaggttatgg ttaatgatac ggtataactt cctgaacctg
    20341 aaaactgatg tgtagcattc agagaattat aaattgtatt atcaatattc cattcaaaaa
    20401 ctgatcctaa ctgatattgc gaagtgttgg tgaaattaat aattggcagt tcacaagtca
    20461 ggctgctgtc gtttgtaaaa gaaactacag gtactggata agcctcaaaa ttatgaattg
    20521 cagaagcttt acatcctgct gtggaagtta ctgtacatac gtagcttccg gaagaattaa
    20581 ctgctataga ttgcgaagtt gctcctgttg accataaata tgctgcaaaa tttcctgtag
    20641 taagtgtggc agttatgcca tcgcactcac ctgcagaacc tgaaatcaca ggttgtggta
    20701 aaggattaac tactactctt gcaactcctg aaacattacc tgtacaattt gcatcattta
    20761 tttgtgtaag cgtataatct gttgtagtac cgggagtcac taaaatttgt gctgctgaat
    20821 tattagtatt taaaattgct gatacacttg tgccatcact ataacgataa ctataaggtg
    20881 cagttccggt aaactgtatc tgtaaagatg tagattgacc aatacaaatg gctgttgaac
    20941 ctgaaattat tgcagtaggt aattgattta tcgtcaatgc aaatggtgct gatgttcctg
    21001 aacatccatt aacatctgtt actgttacaa tatagtttgc accatttgaa acagcaattg
    21061 aatctgtagt tgctcctgtt gaccattgat atgcagaata atttccagct gataaagtag
    21121 tttgtgtgcc actgcagatt gaattatttc cggtgattac gggcgttgga agattccaca
    21181 ctgtaactgc ctgatttgtt gaagctgtac atccattagc atcagtaacg gtcacaacat
    21241 aatttccttg agctgttact gttattgcat tagatgttgc agaagtattc cattgatacg
    21301 aagtaaaact acctccgcca gtagtcagca ctgaggaatt acctgaacaa aatgcagaag
    21361 tacctgaaat tataggaaca ggaagtggat tcacaacgac attaaacgaa gttgatgcaa
    21421 cacaaccatc tgtattggtt accgtaacag aataagttcc tgactgagtt actgatatag
    21481 tttgtgatac agcacctgtt gaccattgcc atgaggcaaa actacctgca tctaatactg
    21541 aattttgatt ctggcaaatt gctgctgttc cactgataac aggaatagga ttttggaaag
    21601 caagtaggtt ataattggat gagcctacac atccgttcgc atctgtaact gttacagtgt
    21661 agtttccgga aacattagta gtaattgatg gcgttgtatt tccatttgac catagataag
    21721 aagcataact tcctgcaaaa agattggcac tagcaccggg gcaaatacca gccggaccac
    21781 ttatggccgg cacaggtaat gcattaacag taataacttt atttgttgtt gcagtacatc
    21841 ccgctgcatt tgtaacggtt actgcgtagt tgccaccagc ggtgacattg atagtttgtg
    21901 tagttgctcc accattccac aaataatttg tgtatccacc gcctgcattg agtgttgagt
    21961 tacctcctgc acaaaatgaa gtcacaccgg ttatggttgg cgttggaata gcattaactg
    22021 caagattaaa tgacgctgtt cctgtgcagc cattggcatc agtaactgtt actgaataat
    22081 ttcccgcagt gtttacatta attgttgccg ttgctgctcc tgttgaccat gaatatcctg
    22141 cataacttcc tgcattgatt gtactgcttt gcccctgaca aatactgctg ttacccgtga
    22201 ttgatggaac agggtttgtg ttgatagttg ttgttgcaga tttacttcct gtacaaccat
    22261 tggcatcggt tactgttact gtaaaagttg aagctgtgtt gactgtaata ttggatgtag
    22321 ttgctccatt tgaccactga taagaagcat aacttccggg tactgtaagt gtagtgtttt
    22381 tacctgaaca gaatgctaaa gtacctgtta cagatggcac cggtaatgca ttaacagtaa
    22441 taactttatt tgttgttgca gtacatcctg atgcatttgt aacggttact gcgtagttgc
    22501 caccagcggt gacattgata gtttgtgtag ttgctcctcc actccacaaa tagttggtgt
    22561 atcccccgcc tgcattgagt gttgaatttc ctcctgcaca aaatgaagtc acaccggtga
    22621 tagttggtgt tgggttggca ttaactgtaa gattaaatga cgcagttcct gtgcaaccat
    22681 tggcatcagt aactgttaca gaataatttc ctgcagtgtt tacattaatt gttgctgcag
    22741 ttgcacctgt tgaccatgaa tatcctgcat agctacctgc attgattgta ctgctttgcc
    22801 cctgacaaat actactgtta cctatgatgg atggaacagg gtttgtgttg atagttgttg
    22861 ttgcagattt acttcctgta caaccattgg catcggttac tgttactgta aaagttgaag
    22921 ctgtgctgac tgtaatatta gatgttgttg ctccatttga ccattgatat gtagtataac
    22981 ttccgggtac tgtaagtgta gtgttcttac ctgcacagaa tgataatgta cctgttacag
    23041 atggcacagg taatgcatta acagtaataa ctttatttgt tgttgcagta catcccgctg
    23101 catttgtaac ggttactgcg tagttgccac cagcggtgac attgatagtt tgtgtagttg
    23161 ctcctccact ccacaaatag ttggtgtatc ccccgcctgc attgagtgtt gaatttcctc
    23221 ctgcacaaaa tgaagtcaca ccggtgatag ttggtgttgg gttggcatta actgtaagat
    23281 taaatgacgc agttcctgtg caaccattgg catcagtaac tgttacagaa taatttcctg
    23341 cagtgtttac attaattgtt gctgcagttg cacctgttga ccataaatat cctgcataac
    23401 ttccggcatt gattgtactg ctctgtccct gacaaatact gttgttgcct gtgatggatg
    23461 gaacaggatt tgtgttggca gtaacattaa cagatgcagt tgcagtacat ccacctgaat
    23521 aggttacagt aacagtatat gtattacttg taccaacaga aacagattgt gtagttgcac
    23581 cggttgacca tagataagaa gcaccagctg ttgctgtaag tgtggtatta cttccggagc
    23641 aaatagttaa cactccatta atagcagcaa caggtgctga ctgtgtaact acaacaggtg
    23701 ttgaaacagc atagcaatta ccaaactgag tgacggtgac agtatatgtt ccggctgctg
    23761 atactacaat agaatctgaa gttgccccat tactccacaa gtaactactt ccgggattag
    23821 cttgtaaaac aactgaacct ccgggacata atgcagtaga acctaatggt gtgatggtgg
    23881 ctgttggtgt tacaaaaatt acttttacct gtttactaga tgcaaaacat gcaggcgaac
    23941 tatttaatac tgcatattga taaacacctg atggtgtaat attagcctgt gtaacaacaa
    24001 tagtttgtgt agtttgacca ttgttccaat tataactgcc ggctggagat gcagtcaaca
    24061 caacagaatc gccatcgcag gcataaactg tatcgaggtt ttgtatgatt tgaactccat
    24121 tagctgcaat tcctacattg ccaatacttg aaaaactttt tgcttctaaa aagactccag
    24181 aatcaaaaac accatcattt acatctgcaa ttgcaaattt catgtgatag gtctgacatg
    24241 gcattacttt tattcctgct gatattacag ttgtgaaacc atcaaaaaag ttggcagtac
    24301 ctccatttgg aacattatct acaaagtttg cacaatgatt acatggcccg gtagctgctc
    24361 cggcactatt gccattattt acattattta ttgaaacagg aattgaagta cccggaatca
    24421 aggctaagtt tttaggacta cctggtatgc ccggtcctga aatatagaaa gcaaaaacat
    24481 cattatatgc tgtgttcaca tagtcgttat actcctcaga agcaaaaaca aacttaaatc
    24541 ctacactgtc tgctgcagga gtaaaatcaa attcaagcac agcagcatcc tgtgtcaggt
    24601 tgggcgatac aattaaattt aattgtgcat cacctgaacc actcatatca ctactatgaa
    24661 aatctgatgg tggtgatgta ggcatttgac ccgtatttcc ggttgagaga aacacgcctc
    24721 ttgtaattcc taaattacat ccacctgtac acctgaaagt acctttagaa tttgcattac
    24781 cggtataggt aacatttgct actgaaacac ctgttcctac aagagtatca acaatagctg
    24841 ctgcaggatt tcctgatgtt acagttaatt gcgcttgcgt gctttgtcct agtaataaaa
    24901 taaaggcagt gcacagatag tttaaaattt tcattttttt ggctttttgg caaaccaaaa
    24961 gtatgatatt ttatttaatc atgcaagtat ttaatgcgca tccagccaat tagagccaat
    25021 tccaatttct acctctaaag gaacttgggt tttaatggca tgaatcatct tttctctgat
    25081 aataggcttt attaaatcaa cctcatcttg atgagcatca aaaactagtt cgtcatgcac
    25141 ctgaagcgtc attttagttt taagattttg tctcttaaat tcctcatgaa tttgaatcat
    25201 tgccagctta accaaatcgg cagcactacc ctgtaagggt gagttaattg cttcgcgttc
    25261 ggcaaatcca cgaacagtaa aatttcttga atttatatcc ttcagataac gtcttctgcc
    25321 cataagtgtt ttaacataac cattatgacg ggcaaactct aatgtctcgc tcatatagtt
    25381 acgaagctta gggtactgaa taaaataatt ctctatcaat tcagcagctt cctttcgtgg
    25441 aattcccaaa cgttgcgata aaccaaaagc agaaatagca tagataattc cgaagttgac
    25501 catctttgct ttgctacgca tctctttagt aacgtcagct aatgcaacat tataaacacg
    25561 tgccgctgtt gcctgatgta tgtcaagtcc ctgcctgaat gcttcaagca tattctcatc
    25621 tccacttatt tctgctgcta atcttaattc aatctgtgaa taatctgctg acaacaatat
    25681 atgttcgctg tcacgtgcaa taaatgcttt acgcacacgc tgtccacgct ctgtcctgat
    25741 gggtatgttc tgaagattgg gcccaataga actcaacctt cctgttgaag caatagtttg
    25801 attgaaagta gtatgtaccc tgccggtttt gacattaacc atctgaggca atgcatcaac
    25861 ataagttgat tttaatttag taagttcacg gtattcaaga atcttctcgg caattggata
    25921 ttcaagtgcc aatttgctca acacttcttc attggttgaa tgttgccccg tagcagtttt
    25981 cttgtcaaca ggtaatttca aatgtccaaa taatacatct cctaactgac gtggtgaatc
    26041 taaattaaat tccaaacctg caatttcaaa aatagctgaa cgatacttca gcatatcttg
    26101 ttccaattct ttagaataag cgttcaaata atccacatct attttaacac cttcatattc
    26161 catatcggcc aatacaggaa tcagtggcag ttcaatatct tttaaaacac cgtcaacctc
    26221 atgtttttca accataggtg ccaacacttg tttcaactga aatgttatat ctgcatcttc
    26281 tgatgcatat tcagcaattt tatcaatagg gacatcacgc atgctcccct gattctttcc
    26341 cttcttgcca ataagttctt caatatgcac aggtgtgtag ccaagataag tctcactcag
    26401 ataatccatg ccatgtttca tatcaggctc aactaaataa tgtgcaatca tggtgtcata
    26461 caaaggtgct ctcacttcaa caccatatcg tttcagcaca agcatatcaa actttatatt
    26521 ttgtcctatt ttaataattg aaacatgttc aagtataggt ttaaatttat gacaaatgtt
    26581 ttttgctttc tcaaagtctt ctggacaagg aatgtaatag cctgttccac tgttgaacga
    26641 gaatgataaa cctacaatac ttaatgaaaa ataatctaaa gaagatgttt ctgtatcaaa
    26701 acaaatttct ttttgtttta ataatactgc aaccaaacca tcaatagctt cattattctc
    26761 taccaacgta taattatgtg gtgtgttctg aatattcttt cctctgacaa catcactttc
    26821 acttcttaca atctcatcat caaataaact agtttgcaca gaagccgttg tggctgtttt
    26881 tatctcacga tttactgaaa aatcttctcc aagaattctt ttacccaaag acctgaattc
    26941 aagctctgta aaaagctgag ataatttttc tttgtcaggc aaagacatta ataaatcatc
    27001 atcattgact tcaacaggaa catcaagaat tattgtggct aattgttttg aaattcttgc
    27061 ctgttcaaca ttactgcgga tattttcacc caacttacct ttaatgtttt ctgcattggc
    27121 aatcacattc tccatactgc catattcttt aattaacttt tttgcagttt tctcacccac
    27181 actgggtatg cccggtatat tgtcaacagc atcgccccac atacccaaaa tatcaattac
    27241 ctgttttgga ttttccactt cccacttctc ctgaatttct ttcactccca aaatttcaat
    27301 gtcgttacct aaacgtccgg gcttataaat aaaaatattg ggcgacacta actgcgcata
    27361 gtccttatcc ggtgttacca taaaaacagt ataaccttct tcctcttttt gtttggccac
    27421 agttcctatt atgtcatcag cctcat
//
LOCUS       DAOOOL010000015        27198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_134_length_27198_cov_130.034152, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000015 DAOOOL010000000
VERSION     DAOOOL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27198
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_27198_cov_130.034152"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(486..857)
                     /gene="rplS"
                     /locus_tag="PKU82_02460"
     CDS             complement(486..857)
                     /gene="rplS"
                     /locus_tag="PKU82_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOZ81766.1"
                     /translation="MDLVKLAEKQLIPVKDAPDFKAGDTITVFYKIIEGNKEREQQFQ
                     GVVIQRKGHGATQTFTVRKISNGVGVERIFPLFSPKITKIDVNKRGVVRRARIYYLRN
                     VSGKAARIEEKIVKTAETKKK"
     gene            complement(984..1670)
                     /gene="trmD"
                     /locus_tag="PKU82_02465"
     CDS             complement(984..1670)
                     /gene="trmD"
                     /locus_tag="PKU82_02465"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOZ81767.1"
                     /translation="MHIDIITVHPGLLEGPFSHSILKRAIQKNLVEINIINLRDYAAN
                     KHKSVDDYAFGGGAGMVMMIEPIDHCITDLKSKRNYDEVIYMSPDGELLNQGISNSLS
                     LKKNIIILCGHYKGVDERVRQHLITREISVGDYVLSGGELPAAVLCDSIIRIIPGVLN
                     DETSALSDSFQDNLLAPPVYTRPANYKGWDVPEVLLSGNESLINEWRHQQSLERTQIR
                     RPDLSDGNSK"
     gene            complement(1673..2458)
                     /gene="mazG"
                     /locus_tag="PKU82_02470"
     CDS             complement(1673..2458)
                     /gene="mazG"
                     /locus_tag="PKU82_02470"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOZ81768.1"
                     /translation="MEEKLNAFKRICTIMDELRTQCPWDKKQTIESLRHLTIEEVYEL
                     SDAILDNNLEEVKKELGDIMLHLIFYSRIAEEKNLFNIADVINTECEKLISRHPHIYP
                     AADGQKTVVADEEEVKRNWEKLKMKEGKISVLEGVPRSLPSMVKAQRIQEKARGAGFD
                     WEKPEQVWEKVEEEFSEFQEAVSRKNQQEMTDELGDVFFALINYARFHNLNPDEALER
                     TNRKFISRFRYMEDKVRESGKTLHELSLSQMDVYWNQAKTTEI"
     gene            2486..3604
                     /locus_tag="PKU82_02475"
     CDS             2486..3604
                     /locus_tag="PKU82_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOZ81769.1"
                     /translation="MIHKAGISFLSTLFLFLLTVAAHGQQATHGAWMLGAEGHYGFVM
                     AHRPSVQPLQHSHLGGMMITLSQQADGKRSWHQHFHFPETGIKYGFFGLGNKEQLGYG
                     HTVYPYIDIPLGKSEKPFLHFQFGWGLGYITKPFNRNDNYQNVAIGSHLNAVIAFNFH
                     SEFWLTKRDRIVPAIGLTHFSNGSAAMPNLGINLITAQLHYSRSFGESKEIIRQETEN
                     FEKRNRFTIFGAWAFKQVYPLAGGTWYAWTLSPAFLRQYSPKSAAGISVDLFYDPTIN
                     YRLAYEDMKTHGTISNFRGGVAGAYEIIFSDFSVILQMGVYAKNAWKKDGLFYNRLGM
                     RYRFYDGLFACINLKTHIARADFIEWGIGYNFNKKAWN"
     gene            3595..4830
                     /locus_tag="PKU82_02480"
     CDS             3595..4830
                     /locus_tag="PKU82_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOZ81770.1"
                     /translation="MELKEILLWIIASILVLNYLLSMSLSILNLLNLSETQPDELKQY
                     YDEERYKKSQQYEREKSRFGMISETVMFVISFFFLIYGGYGWLDGWTHTVTTDLLWST
                     LLFFGTFALFSDLISIPFSLYSTFVIEERFGFNKTTLAVFISDRIKGYFLAGALGGSL
                     LAMFVLFYQYTGSNFWIYMLAVFAVFMIVLNMFYTSLILPLFNKLTPLADGEVRTAIE
                     NYCQKNKFEITNLYVMDGSRRSSKANAFFSGLGKKKKIVLFDTLVNNYSKDELVAVLA
                     HEVGHYKKKHTLFSMIFSILQTALLLWLLSVFINNEWFSLALGGTEYSLALSLIAFTL
                     LYSPLSLITGILMNIYSRKNEFEADNFARVTFSAEALASALKKLSSDSLSNLTPHPMY
                     VFFYYSHPPLLQRLKALQA"
     gene            4971..5768
                     /gene="cdaA"
                     /locus_tag="PKU82_02485"
     CDS             4971..5768
                     /gene="cdaA"
                     /locus_tag="PKU82_02485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOZ81771.1"
                     /translation="MELFHIGFLTVRLLDILDVLIVAWVLYKLYRLLKGGVAMNILIG
                     ILTIYFLWWLFVKVLDMQLLGALFGQFIGVGVIALIIVFQQELRRFLIFVGSNTIFTR
                     GDFFKNLLHGNFKLQQPVELSVMPIVRACVSMSKTKTGALIVVTRKTDLNLFEGTGDI
                     MEAEVSKRLLESIFFKNSPLHDGAVIISGNRIRAARCVLPVTESLDVPAHYGMRHRAA
                     LGITEQSDALALVVSEENGQISIAAGGQFYSNLSPDELEKKLKELME"
     gene            5971..7146
                     /locus_tag="PKU82_02490"
     CDS             5971..7146
                     /locus_tag="PKU82_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOZ81772.1"
                     /translation="MPSVSFNNLKVAFAYKSDADLKRAYWLFRLININFLVKIGPGFT
                     NAVLKMGLPIKPLIRATIYKHFCGGETIEQCTETINNLGKYRVGSILDYSVEGHHNES
                     DFDHSCAEIIKTVKHASGKKNIPFSVFKVTGVGRMDLLERVSSKVLLSEAEEAEFLRL
                     RARVESICKTAAENNVRLFIDAEETWIQDAIDNMVDDMMLKYNKEKTLIFNTVQLYRH
                     DRVAFMRENIAKARSGNYRVGYKLVRGAYMEKERERAAVNGYPSPIQPDKAACDHDYN
                     EAIKLCIENIDMVAVCAGTHNEDSSALLAKLMEEKGIANSDERVWFSQLLGMSDHISF
                     NLANAGYCVCKYVPYGPVVSVLPYLFRRAAENTSMSGQMGRELSMITQEIERRKKEK"
     gene            complement(7148..8188)
                     /locus_tag="PKU82_02495"
     CDS             complement(7148..8188)
                     /locus_tag="PKU82_02495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ81773.1"
                     /translation="MQTDYIIVGNGLAGSLLAWHLLLQEKNVVIIDEFNNTVASQVSA
                     GIFLPITGRRIVRTWMADELLAEVKTIFTQLGKLIGETYLYEMPVVEIPASVKEWNEW
                     QQRASSPDISKYISRFLAPNSIESVHCPFGAIELKNSGYVNISKCVTFLRKLYLDMEI
                     IIEGKFNFDDLQYSDKIVYKDIVADKIIFCEGAAGINNPFFNYLPFQLSKGEILTIRC
                     NTLSEEQIFNHGIFILPIGNQLFRVGSTYEWNDLTTNTTESARNNLSEKLQTVLKSDF
                     EIVDQKAGIRPSVKERRPFIGIHPKHKNIGIFNGLGTKGVMLAPYFAKHFAWHLAHQK
                     PLMADVDIARFS"
     gene            complement(8278..9243)
                     /locus_tag="PKU82_02500"
     CDS             complement(8278..9243)
                     /locus_tag="PKU82_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81774.1"
                     /translation="MTRFYLSVLAIFSVLTTSAQQMSTDIFLTNFGMNREGQYFFTAP
                     QNITHRPGYDNQPSFSKDGSKVYYVAYYDTLQSDIYVYSLEDSTNTRLTETPESEFSP
                     RVTADDLGFTIVRVDADKGQRFYKVLMDGTNEFQLLGVSDSVAYYCIANDSTYAVADL
                     NNNVMELNIYEMPSEQFIPLAKNIGRCIAVIPDGDNEISYVDKADTNGYMLMSFSINT
                     GLIGSVCQLKKGVEDYVWTKDGKLLIGYEGKLLMFDRNKPDNGWTELADFSKSVGNFY
                     RIAISQQGTQLALVAYTKEDKKQDDAKDDSGKKDDKKKRKKKKDE"
     gene            complement(9240..9911)
                     /locus_tag="PKU82_02505"
     CDS             complement(9240..9911)
                     /locus_tag="PKU82_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81775.1"
                     /translation="MVALLNFLLITIISCFVVSLILLMVSPRIPVFFSYIAIALHIIF
                     ITLYLIYRNSVHEERVVMLFAAIICSGIIPFTLIVRRNFPLILKLYFGLFMLSLPLFI
                     IAPSKMLTILSMGYLNTENAHEMHLDKNYYLSKEQGLIKQKDNEATYKVVKRMGIFNK
                     TLVRNIFIGFVPDSAKILFLDEKSEIRLRAYMHGKTEIDSLDITTKTVASRDTILKII
                     KTQDK"
     gene            complement(9922..10656)
                     /locus_tag="PKU82_02510"
     CDS             complement(9922..10656)
                     /locus_tag="PKU82_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOZ81776.1"
                     /translation="MLNPKLSDKKTKGILYLIPTAIGSMQAIDYLPQTVIEIIHSLDE
                     FIVENSKSARQFLKHCHIPTPQQQLTIHELNKHEGNVFEASFFSNLLSGKNVGLLSEA
                     GCPAVADPGALIVAEAHKLQIKVVPLTGPSSILLALMASGLNGQQFAFNGYLPKEKTE
                     RFAAIKKAEKDSAQRRQTQIFIETPYRNNALFDDLLNNLQPSTRLCIASALQCENEMI
                     KTQRISDWKKQRQELNKHPVVWLFLA"
     gene            complement(10634..11113)
                     /locus_tag="PKU82_02515"
     CDS             complement(10634..11113)
                     /locus_tag="PKU82_02515"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOZ81777.1"
                     /translation="MKVKILMVCLGNICRSPLAEGIMQHKLNQAGIAAQVDSAGLINY
                     HTGELPDKRSIAVAAKYGIDLTAQRARSFSKDDIKNFDYIFAMDNKNFENLMEYANNA
                     AEAKRIHLILDYAGLGKKIVPDPYYGDQSDFENVYSLLDDACQRILKKFAYAQSEVV"
     gene            complement(11109..12557)
                     /gene="dnaA"
                     /locus_tag="PKU82_02520"
     CDS             complement(11109..12557)
                     /gene="dnaA"
                     /locus_tag="PKU82_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOZ81778.1"
                     /translation="MLKQTEKNVEKVWENCLKLIRDNVSPQNFKTWFEPIKPVKLEKH
                     VLTIEVPSQFVYEWLEEHYIQLLRKSIKKELGAEGRLEYSILMENNMNSSKPYAVKIP
                     AKGTGNLKNPQVAMPVSLNTAIKNPFIVPGLKKIDVDSQLNPNYSFDNFIEGDCNRLA
                     RSAGYAVANKPGGTSFNPLLLYGGTGLGKTHLAHAIGIEIKNNFQNKTVLYVPADRFM
                     NQFVDAVKNNSPNDFVHFYQMMDVLIIDDIHFLAGKERTQDVFFHIFNHLHQNNKQII
                     LTSDKAPVDLQGIEQRLLSRFKWGLSADLQSPDLETRIAILKRKMYNDGIELPTEIVE
                     YIAYSITTNIRELEGALISIIAQTSLNKKPITLELAKQMIDKFVKNTVHEISIDYIQK
                     IVCDYFELPLETLKSKTRKREVVQARQLAMYFSKSLTKSSLSAIGAHCGGKDHATVLH
                     ACRTVNNLMETDKKFKGYISDLQKRINLNHKN"
     gene            complement(12720..13655)
                     /locus_tag="PKU82_02525"
     CDS             complement(12720..13655)
                     /locus_tag="PKU82_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOZ81779.1"
                     /translation="MTNRRDFLRTFAVGAAGLSLTGLPLEALAKRRVTKITILHTNDV
                     HSHIDPFPANDPKYPGLGGVAQRAAIINDIRNKERNVLLLDAGDIFQGTPYFNLFGGE
                     VELKLMSQLGYDAGTIGNHDFDNGVDGLSSQLKHANFPLIICNYDFKGTSMEGKTIPY
                     KIFEKEDVRIGVFGLGIELEGLVDKKMYGETKYLDPLEKAAETAHHLKFNEKCDLIIC
                     LSHLGYRYPNKKVSDDVLAKKSLNIDLIIGGHTHTFIDKPFVYKNRDNKDVLVAQVGW
                     AGIKLGRIDFFVDKKMRKLTADSTTLKVLNYARAI"
     gene            complement(13645..14403)
                     /locus_tag="PKU82_02530"
     CDS             complement(13645..14403)
                     /locus_tag="PKU82_02530"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOZ81780.1"
                     /translation="MSFRNILLAAAIVFTSCKQNAHLVKTETTTYPLNKPGFTEVDSS
                     ILNSYSSYKHTIDSIMDRVIIVSEMSMQKKTPEGLLGNFTSDAALKQVENDFHAKDFS
                     KIDFCFLNNGGLRAALPKGDITIGNIYSLMPFENEMVLLTLNGATTLKLINFIAAKGG
                     MPVSGLTMRVHESTVTEAKIQGMPFDSTKSYVVATSDYLANGGDNLSFLADAEKKVST
                     GLKLRDLLIQYLEEKNKRGEKLSAKIEGRIIYDK"
     gene            14619..15122
                     /locus_tag="PKU82_02535"
     CDS             14619..15122
                     /locus_tag="PKU82_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81781.1"
                     /translation="MHPHFKLLLAVFVLFSISSQAQRIDKSFRIKVSDNRNLQFTSYE
                     YLLKDDSMKISGVSDYGKSRVVYIAKKLKSKQKKAIRNFLKDFPVDSLQKEYFDDYAN
                     FGYIASDHFPRVIELEIRTGLKQHFSKASNAYVRSYARLIDFLNTLINDDVVKINYPE
                     SDFKKTF"
     gene            15126..16433
                     /gene="rimO"
                     /locus_tag="PKU82_02540"
     CDS             15126..16433
                     /gene="rimO"
                     /locus_tag="PKU82_02540"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOZ81782.1"
                     /translation="MRTKTSKQLKVNVVTLGCSKNVVDSEVLMGQLRANEIEVEHESK
                     RQSEIVIINTCGFIDNAKQESIDTILHYAKEKEKGKIEKLIVTGCLSERYKPDLEKEI
                     KGIDNIFGTRDLPLLLKTLGADYKHELIGERLVTTPRHFAYMKISEGCDRPCSFCAIP
                     LMRGNHISKPIEELVHEARHLAAKGTKELLLIAQDSTFYGLDIYKERKLANLMEQLCS
                     VDGIEWIRLHYAFPSGFPLDVLDIMQRENKICKYLDIPLQHASDNMLKSMRRGITREK
                     TITLLDTVRNKIPDIAIRTTFISGYPGETEQDHETLIRFLEEMRFERVGVFTYSHEEN
                     THAYTLNDDVDEATKQRRLDELMQVQQNISDSINQKKVGNVFKTLFDRKEGNFFIGRT
                     EYDSPEVDNEVLVDANENYVRVGDFANIKITSAEAFDLYGTLC"
     gene            16479..16997
                     /locus_tag="PKU82_02545"
     CDS             16479..16997
                     /locus_tag="PKU82_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOZ81783.1"
                     /translation="MKLLLIMFAFLFGPASTATSIYEFKVDGLSGGQIDFSKYKGKKI
                     LIVNTASKCGYTPQYSDLEKLYEKYKDKLVIVGFPANNFLWQEPGSNNEIAEFCKKNY
                     DVTFPMAAKVSVKGRDMHPLFRWLIEQPNVDFTGGIKWNFEKFLIDENGKLIHRFRSS
                     VLPTSPEITKYL"
     gene            complement(17003..18877)
                     /locus_tag="PKU82_02550"
     CDS             complement(17003..18877)
                     /locus_tag="PKU82_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ81784.1"
                     /translation="MLLRESIVRNKVNSFCTSFHNKTGGNINIGKIQFNGLRVIEIEN
                     ISLVSPLNDTLLHTDTFMVQLSLLKLIRLKNPVSNFSAHGIKIKIASGDSISNYSFLF
                     RKSKPENTEITSVQSTQASKVFTLWNKLTELSDLSFDLERCKITIIRNKETRNFYFPE
                     IINRRGKCRFIAYSNDHLQTEKWQAGFNIDDDKNEMSLNIKKLAGENWLPFFDVDNKP
                     KIAYSAISLQIKQPSRKDTTFQVKAELDSLSVNYWRIANDDVDFNKLSFSGICSFNNQ
                     RIETDSAVNITINKIHFKTKAAFDRKQPHSYALSLAFDESADDFFESLPKGIFYHFKN
                     FKAKGRLKFDLNFALDMSAPDSLIFNSDLTGNALTIEKYGDENFSRINNTFLFDAYDG
                     DRLQRSFLIGPENSDFTPLNEISHYLQYAVLTSEDPSFFYHRGFIPDAFKESMIENIK
                     AKRFVRGGSTISMQLVKNVFLSREKTISRKLQEMLIVWLIENKGLVSKERMFEIYLNA
                     IEWGPGIYGIKEASQFYFNKLPSQLNLAESIFLASIIPRPKYYRYSFDAQGNLKSYLA
                     SYYRTVSNRMLSKTWISPIDTFNLQPQVKLKGPALKIVMPADSSMTDSLQWNQSNELF
                     "
     gene            complement(19001..20983)
                     /locus_tag="PKU82_02555"
     CDS             complement(19001..20983)
                     /locus_tag="PKU82_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ81785.1"
                     /translation="MLLRESIVRNKVNSFCTSFHNKTGGNINIGKIQFNGLRVIEIEN
                     ISLVSPLNDTLLHTDTFMVQLSLLKLIRLKNPVSNFSAHGIKIKIASGDSISNYSFLF
                     RKSKPENTEITSVQSTQASKVFTLWNKLTELSDLSFDLERCKITIIRNKETRNFYFPE
                     IINRRGKCRFIAYSNDHLQTEKWQAGFNIDDDKNEMSLNIKKLAGENWLPFFDVDNKP
                     KIAYSAISLQIKQPSRKDTTFQVKAELDSLSVNYWRIANDDVDFNKLSFSGICSFNNQ
                     RIETDSAVNITINKIHFKTKAAFDRKQPHSYALSLAFDESADDFFESLPKGIFYHFKN
                     FKAKGRLKFDLNFALDMSAPDSLIFNSDLTGNALTIEKYGDENFSRINNTFLFDAYDG
                     DRLQRSFLIGPENSDFTPLNEISHYLQYAVLTSEDPSFFYHRGFIPDAFKESMIENIK
                     AKRFVRGGSTISMQLVKNVFLSREKTISRKLQEMLIVWLIENKGLVSKERIFEKNFMP
                     IGRMNLLPKGKEITSDPEFTTQMRYLCISPLVLFDPIKDAARSTEQIDPTSHNFSDIL
                     FNTTLDAMEKSGVPEATISSYAEFDVQPDAEYMQKAAAANKKFARVYRSAEGKNMIGY
                     LVPFTLHAHPDVHRYVWHNGIGVMTHEGYGMLDLAK"
     gene            complement(21107..21841)
                     /locus_tag="PKU82_02560"
     CDS             complement(21107..21841)
                     /locus_tag="PKU82_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOZ81786.1"
                     /translation="MRVKISFTRDNTSGNSIPLHHQKLIYDSLKTVVHEVGGNMKQLN
                     FSSLKGTSKIQNGFMKFLSSKITLVISGSNDDLITKVSNRIFEKNFMPIGRMNLLPKG
                     KEITSDPEFTTQMRYLCISPLVLFDPIKDAARSTEQIDPTSHNFSDILFNTTLDAMEK
                     SGVPEATISSYAEFDVQPDAEYMQKAAAANKKFARVYRSAEGKNMIGYLVPFTLHAHP
                     DVHRYVWHNGIGVMTHEGYGMLDLAK"
     gene            complement(21953..23044)
                     /locus_tag="PKU82_02565"
     CDS             complement(21953..23044)
                     /locus_tag="PKU82_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOZ81787.1"
                     /translation="MLKSALNLLLWLITIFIAVVTFMAVAAAYISPAKIWFLALFGLI
                     FIYIYLVAFIWMLVLSFRHKKMMLLILIPLIAGLPTFVAHWNYSSSNQKATGENTIKV
                     LSFNVRVFDLYNWTHNLQTRSNIFNFLKKSDAGIYCFQEYYTSKLMPFNNTDSLKSLL
                     GISNVHTMLPIIERDSDEWGIATFSKYPILNKETVFTDKESANGCIATDLLIGNDTVR
                     VYNIHLQSVRLAKNDYIFVSEFNNKDNTQKMKGSKQIVQRLKKAFIKRAHQVCKVTTH
                     IGACPYKVIVAGDFNDTPASYAYNQISNGLTDSFLSCGKGLSSTYAGMFPGLRIDYIL
                     HSKSIKTIEYIRPLVHLSDHYPVISTLNF"
     gene            complement(23044..23916)
                     /locus_tag="PKU82_02570"
     CDS             complement(23044..23916)
                     /locus_tag="PKU82_02570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF020140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ81788.1"
                     /translation="MKSNYKSLDMLQKIIVVNAIIYLFVNIGDVVLRLFNFPDGTVSG
                     FLTDWLAVPSSLKSLLYRPWTLITYCFYHENFLHILFNMLWLFFMGRLFTEYLGEKKL
                     LPLYLVGGLCGSVLFIVFYNLFPLFSREVDIAVALGASASVLAVTVAIATLLPDYEVG
                     LFLPQWRVKLKYIAIALFVIDLLSITGNNAGGNIAHIGGAIFGFIYIKQLKKGRDLTG
                     WLQRMIDRITQKKKPSIKVVYRKDSSDESYHNKKANNQEAIDKILDKISRSGYASLTN
                     EEKEILFKASKNQD"
     gene            complement(23931..24770)
                     /locus_tag="PKU82_02575"
     CDS             complement(23931..24770)
                     /locus_tag="PKU82_02575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ81789.1"
                     /translation="MQEFRPSSFKLLPDVIKNIMIVSALAFFADLALNKIGFDLNAYA
                     GLYLPSSEHFHWWQYFSYMFLHGSFSHILLNMFAFWMFGGILENLWGPKRFLIFFLVT
                     GLGAAFIHTAVGYFELHSLQAKADAFFNAPGADAFSVFVRDHIPYQYLNADYAMAVEN
                     IKSNWYAHGDSPMIIEQARQAIDEYIRFKTNIPTVGASGAVYGVLMAFGILFPNTLIY
                     LYFFIPLKAKYLVLIIGGIELYSAWQSNPTDNVAHFAHLGGMLFGYLLLRYWQKNNRS
                     QLF"
     gene            complement(24877..26670)
                     /gene="mutL"
                     /locus_tag="PKU82_02580"
     CDS             complement(24877..26670)
                     /gene="mutL"
                     /locus_tag="PKU82_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOZ81790.1"
                     /translation="MADVIQLLSDTVANQIAAGEVILRPSSVIKELMENAVDAGATRI
                     DVSINDGGKNLIRITDNGCGMSETDARLCFERYATSKLRKADDLYAIRTMGFRGEAMA
                     SVAAVCQVELKTKREEDETGTRIEIEGSEFKIQEPCVMQSGTIITVKNIFFNVPARRN
                     FLKATPVEARHVMDEFLRVALPRPEIGFSLQHNGMEVYNLPATHQRQRICQVFGATYN
                     ERLVPVEEDTTILNIKGFVGKPEFAKKVRGEQYFFINKRFVKDAYLNHAVTIAFNELL
                     PQGTFASYFLFFEVDPSRIDVNIHPTKTEVKLEDEKSVYAILRSSVKRALGRFSIAPT
                     LDFNQEQAFNVPLSQLNTIPVQPQVKVNTNYNPFKSTEINKPTQQQNGQWEKLYEIAS
                     TPDVQQTKLPIAPIEENNASAFMQLFNSYVAVAMPDEMWLIDQQLAHEKIVFEKTLLQ
                     MKGTPTVMQQSLFPQQIELDNRQAATVSSLLEDLNRSGFDLQPFGQNTFVLNAVPSYL
                     ETGTEKKIIEMLIDEFISGEASAYNPMERVAIAIARSACLKSGKVLSAREMSALFNDL
                     MQCEMPYFTKSGKAIIFKLEKNEIDRKFEKG"
     gene            complement(26781..>27198)
                     /locus_tag="PKU82_02585"
     CDS             complement(26781..>27198)
                     /locus_tag="PKU82_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81791.1"
                     /translation="SDGVERSTAFNGNFVFNLLGGKEWTIRKKNTISINIKTTYAGGR
                     RYIPIDFAASQITGETEYDEDHAFEHRRKDYFRTDLKIGYRINRKRLTHEIAIDLNNV
                     FNTQNIWNQQYSAKTNTVKTEYQMGFLPIPMYKCTF"
BASE COUNT         8356 a         4791 c         5094 g         8957 t
ORIGIN      
        1 gaataatgaa gtacttagat aatttgaaaa ttaggtaatt tgataatttg ataatgcaga
       61 gtgcttgcac tcactcgtaa ccaaaagaag ctttactcta attagaatag atttaatgct
      121 atggatagaa atatgtttag cggacagaca ccccttcagg ggttgggggt gagcagagga
      181 atgcagattt tttctgaata atgaagtact tagataattt gaaaattagg taatttgata
      241 atttgataat tcagaatgct tgctcctact taaaatagca atgctaatca atttaccgat
      301 ttaaagctgg ctgaactatg aagtccgata gctatcggac ggccagaggc cgcaaggata
      361 aaaggcacga gcggaaagga cactcgcgcc tacttagctc gcgcctactt agtctttggg
      421 aaagtgtact cataaaaaaa gccccgttat tacgaggctt ctttacaaat caaaatgaaa
      481 cttttttatt ttttcttggt ctcagctgtc ttaacaattt tttcttctat acgcgcagcc
      541 ttacctgaaa cattacgcag gtaatagatt cttgctctgc gaacaacacc gcgcttgttt
      601 acatcaatct tggtgatttt aggactgaat aatgggaaaa tacgctcaac gcctacacca
      661 ttagaaattt tacgaacggt aaatgtctgt gttgcaccat gtcctttacg ttgaataaca
      721 acaccctgaa actgctgctc acgctcttta ttaccttcaa taattttata gaatacggta
      781 atggtatcac cggctttgaa atccggagca tcttttaccg gtattaactg cttttctgct
      841 aatttaacta agtccatcgc tttatatttt gatgattgat aaaattttgg gagtgcaata
      901 atagcaaaaa aagctttagg gtgataagat ttatactaac tttttaaaaa atgtttaaaa
      961 aattgtattt tattgaaaaa cagttatttg ctattgccat ctgacaggtc tggtcttctg
     1021 atttgtgtac gttccaggct ttgctgatgc cgccattcat taataaggct ttcattaccc
     1081 gacaaaagca cttcaggaac gtcccagcct ttgtaatttg cgggtcgggt ataaacaggc
     1141 ggtgccaaaa ggttatcctg aaaagagtcg gacagggcag aggtttcatc attcaaaacg
     1201 ccaggaataa ttctgatgat gctatcacaa agtacggctg caggtagttc gccaccggaa
     1261 agaacataat cgccaacaga aatttcgcgt gtaataagat gttgccgcac tctttcatca
     1321 acacctttgt aatgaccaca gaggataatg atattttttt tcaacgacag gctattgctt
     1381 attccctgat tgagtagttc gccatcgggc gacatgtaga tgacttcatc ataatttctt
     1441 tttgatttaa ggtctgtgat acaatggtca ataggttcta tcatcatcac cattccggca
     1501 ccaccgccaa aggcatagtc atcaacactt ttatgtttgt tggcagcata gtctcgaagg
     1561 ttaatgatat ttatttcaac aagatttttc tgaatggctc ttttaagaat agaatgactg
     1621 aaaggccctt caagcaaacc gggatgtact gtgataatat caatatgcat catcagattt
     1681 cagtggtttt tgcctggttc caataaacat ccatttgtga cagtgagagt tcatgtaaag
     1741 tttttcctga ttctctgact ttgtcttcca tatatctaaa acgcgaaata aattttctgt
     1801 tcgtgcgctc caatgcttca tcgggattca gattgtgaaa acgtgcatag tttatcagtg
     1861 caaaaaaaac atcacccaat tcatcggtca tttcctgttg atttttacgg gagacagctt
     1921 cctgaaattc actgaactct tcttcaactt tttcccatac ctgctccggt ttttcccagt
     1981 cgaagccggc accacgagct ttctcctgaa tgcgctgcgc ctttaccatg ctgggtaatg
     2041 aacgaggcac accttccaat acagatatct tgccttcttt cattttcaat ttttcccagt
     2101 tacgttttac ttcttcttca tcggcaacaa cagttttttg tccatcggca gcaggataaa
     2161 tatgtgggtg gcgcgaaatc agtttctcac attctgtatt aatcacatca gcaatgttaa
     2221 acaggttttt ttcttcggca attcgtgaat aaaaaatcaa atgcaacata atgtcgccca
     2281 attctttttt aacctcttct aaattgttat ctaagatggc atcactcaac tcataaacct
     2341 cttcgatggt gagatgtcgc aacgattcta tggtttgctt tttatcccac ggacactgtg
     2401 tgcgcagttc gtccatgatt gtacaaattc ttttaaaagc atttaatttt tcttccattg
     2461 ctaaaagcat tttctttgca aaggtatgat tcataaagca ggcattagtt ttctgtccac
     2521 actattttta ttcttgctga ctgttgcagc acatgggcag caagccacac atggtgcatg
     2581 gatgctgggt gcagagggtc attatggttt tgttatggca caccggcctt ctgttcagcc
     2641 actacaacac agtcatcttg gtggtatgat gattacctta tcacagcagg cagacggcaa
     2701 acgatcatgg catcagcatt ttcattttcc ggaaacagga attaaatatg gttttttcgg
     2761 attgggtaat aaagaacagt taggctatgg gcatacagta tatccttaca ttgacattcc
     2821 attaggcaaa tcagaaaaac catttttaca ttttcaattc ggatggggat tgggatatat
     2881 taccaaaccg tttaacagaa atgataatta tcagaatgta gctataggat cgcatctgaa
     2941 tgctgttatt gcatttaact ttcactcgga gttttggctt actaaacgcg acagaattgt
     3001 tcctgccata ggtttaacac atttttcaaa tggaagtgcg gcaatgccaa atctgggaat
     3061 aaatttaatt acggcacaac ttcattacag ccgtagtttt ggtgaatcaa aagagattat
     3121 caggcaagaa actgaaaatt ttgaaaaaag aaatcgcttt acaatttttg gagcatgggc
     3181 tttcaaacaa gtttatccgc ttgccggagg cacctggtat gcatggactc tttctcctgc
     3241 atttttaagg cagtacagcc ctaaatctgc ggcaggcata agtgttgatt tattttatga
     3301 cccgacaatt aattacagac ttgcgtatga agacatgaaa acacatggaa caatatcaaa
     3361 ttttcgtgga ggtgttgcag gagcgtatga aataatattt tcggattttt ctgtcatatt
     3421 gcagatgggt gtttatgcaa aaaatgcatg gaagaaagat ggtttgtttt ataacagact
     3481 gggaatgcgc tatcgttttt atgatggatt gtttgcctgc atcaacctga aaacacatat
     3541 tgcaagagcc gattttatag agtggggaat aggatataac tttaataaaa aagcatggaa
     3601 ttaaaagaaa tattgttgtg gataattgca tcaatactgg tgctgaatta tctgttgagt
     3661 atgagtttgt ctattttaaa tttattaaac ttatcagaaa cacaacccga tgaactgaaa
     3721 caatattatg atgaagaacg gtataaaaaa tcacagcaat atgagcgaga gaaaagccgc
     3781 ttcggaatga ttagtgaaac ggtcatgttt gttatttctt tcttttttct gatttatggt
     3841 ggttatggtt ggcttgatgg ttggacacat acggtaacta cagatttgtt atggtccacc
     3901 ttgttgtttt ttggaacttt tgctttgttc agtgatttaa taagcattcc gttttcactt
     3961 tattcaacct ttgttattga agaacgtttt ggatttaata aaacaacact tgctgttttt
     4021 atttcagaca ggattaaagg atattttctt gcaggagcat taggtggaag tttgttggcc
     4081 atgtttgttt tgttttatca atatacagga agtaattttt ggatttacat gcttgcagtt
     4141 tttgcggttt ttatgattgt attgaatatg ttttacacct cattgatact tccattgttt
     4201 aataaactta ctccattggc agatggcgaa gtacgtactg caattgagaa ctactgtcag
     4261 aaaaataaat ttgaaattac taacctttat gtgatggatg gtagcagacg ttcatcaaag
     4321 gcaaatgcct ttttcagtgg gttaggcaaa aagaaaaaga ttgttttgtt tgataccttg
     4381 gttaataatt attcgaaaga tgagttggtt gcagtgctgg ctcatgaagt aggtcattac
     4441 aagaaaaagc atacactgtt ttcaatgata ttttcaatcc ttcaaactgc tttgttgctg
     4501 tggctgcttt ctgtatttat caataatgaa tggttttctt tagctcttgg cggaacagaa
     4561 tacagtctgg ctttaagtct tattgcattt actttattgt attcaccctt atcactaatt
     4621 acaggaatct tgatgaatat ctattccaga aaaaatgagt ttgaagcgga taattttgca
     4681 agagtaacat tcagtgcaga agcattagca agtgcattaa aaaagctcag ttcagatagt
     4741 ttgtcaaatc ttactccgca tcccatgtat gtatttttct attactcaca tccgcctttg
     4801 ttgcagcgat taaaagcatt gcaagcttag ttttatacag aatgattttt attttttaga
     4861 actataggta atagttcaaa atgaagtgtc aacaacattg tgcgaaaaaa attatgaaac
     4921 aacagtaaag ttgaaataaa cttctgttat ttgtaatcat agcgacaggc atggagttgt
     4981 ttcacatagg atttttaacc gtaagactgc ttgatatact tgatgtatta attgtagcat
     5041 gggtgctgta taaactttat cgtttactaa agggaggtgt tgcaatgaat atcctcatag
     5101 gcattcttac catatatttt ctgtggtggc tttttgtgaa agtactcgac atgcaattgc
     5161 ttggtgcttt gtttgggcag tttattggag ttggagttat tgcacttatt attgtttttc
     5221 agcaggaact acgcaggttt ttaatttttg taggctcaaa tactattttt acaagaggtg
     5281 atttttttaa gaacctgttg catggaaatt tcaagctgca acaacctgtt gagttaagtg
     5341 taatgcctat tgttcgtgcc tgcgtttcaa tgtctaaaac aaaaacagga gcgttgatag
     5401 tagtgaccag aaaaaccgat ttgaatttgt ttgaaggtac cggtgatatt atggaagctg
     5461 aagtttccaa acgactttta gagagtatat tttttaaaaa cagccccttg catgatggtg
     5521 ctgtaatcat cagtggcaac agaattcgtg ctgcccgctg tgttttgcct gttactgaaa
     5581 gtttagatgt gcctgcacat tacggaatgc gccacagagc tgcattaggt atcacggagc
     5641 agagtgacgc tttagcgtta gttgtgtcgg aagaaaacgg gcagatttct atagctgcag
     5701 gcggacagtt ttactccaac ctttcaccgg atgagttgga aaagaaattg aaagaattga
     5761 tggagtgagg ttgtactgct ttcatataaa caaaccattc ttgtcttttt tctcgaataa
     5821 ctttccaaac acattccaat agtttgacaa attggttaaa gtaaggttaa atcaaagtgt
     5881 taagctgttg attaatatca tatttacctc ttacagaaat tagatttacg gatattttaa
     5941 ttcaattatc tttggcaaag aatttttaat atgccatctg tatcattcaa caatctgaag
     6001 gttgcatttg cttataaaag cgatgcagat cttaaaagag cctattggtt attcaggctc
     6061 atcaatatta actttctcgt aaaaattggt ccgggattca ccaatgcagt tttaaaaatg
     6121 ggtttgccga taaagccatt gataagagct accatttaca aacatttttg tggaggagaa
     6181 accattgagc aatgtactga aacgataaat aatctcggaa agtatagagt aggctcaatt
     6241 ttggattatt ctgtagaagg ccatcacaat gaatctgatt ttgaccatag ctgtgcagaa
     6301 attattaaaa ccgtaaagca tgcaagcgga aagaaaaata ttcctttttc tgtatttaaa
     6361 gttaccggtg taggcaggat ggatttgtta gagcgtgtca gcagtaaggt tttactttca
     6421 gaagccgaag aggcagaatt tttgagatta agagcaagag ttgaatcaat ttgtaagaca
     6481 gcagcagaga ataatgtgcg tttatttatt gatgcagaag aaacatggat tcaggatgcc
     6541 attgacaaca tggttgatga catgatgttg aaatacaaca aagagaagac acttattttt
     6601 aataccgttc agctttatcg tcatgacagg gttgcattta tgagagagaa tattgcaaaa
     6661 gcccgttcag gtaattacag agtagggtat aagttagtgc gcggtgccta tatggaaaaa
     6721 gaaagagaac gcgcagcagt aaatggttat ccttctccta tacagccaga taaagcagcc
     6781 tgtgatcacg attacaatga ggctataaag ttgtgcattg aaaatataga catggttgct
     6841 gtttgtgccg gcacacacaa tgaagatagc agtgccttac ttgcaaaact tatggaagaa
     6901 aaaggtattg caaattctga tgagcgtgtt tggttttcgc aactgcttgg catgagcgat
     6961 cacattagtt ttaatcttgc taatgcaggt tactgtgttt gtaagtacgt gccttatgga
     7021 cctgtagttt ctgtgctgcc ttatttattc agacgtgccg ctgagaatac cagcatgagt
     7081 ggtcagatgg gacgcgaatt gagtatgatt acacaagaga ttgaaagacg taagaaagaa
     7141 aaatagttta agaaaaacga gctatatcca catcggccat taacggtttt tgatgtgcaa
     7201 gatgccaggc aaaatgtttt gcaaaataag gagcaagcat cacaccttta gtgcccagcc
     7261 cattaaatat tccaatgttt ttatgttttg gatgtatgcc tataaaaggt cttctctctt
     7321 ttacacttgg tctgatgcct gctttctgat caacaatttc aaaatcggac tttaatactg
     7381 tctgcaactt ttcagataga ttatttctgg cactttctgt tgtgtttgtt gttaaatcat
     7441 tccattcgta agttgaaccc actctgaata gctgattccc tattggcaga ataaaaatac
     7501 catgattaaa aatctgctct tctgaaagtg tattacaacg tattgtcaga atttcacctt
     7561 tggagagttg aaagggcaga taattaaaaa atggattgtt tataccggca gcaccttcac
     7621 agaaaataat tttatcggcc acaatatctt tataaactat tttatcggaa tactgtaaat
     7681 catcaaagtt gaattttcct tcaatgataa tttccatatc taaataaagt ttccttaaaa
     7741 aggtaacaca tttagaaatg tttacatagc cgctgttttt taattctatt gctccaaatg
     7801 ggcaatgaac agattctatt gaattaggtg ctaaaaaccg gctgatgtat ttgctgatgt
     7861 caggacttga tgcacgctgt tgccattcat tccactcttt aacagaagcc ggaatttcaa
     7921 ctacaggcat ttcatataaa tatgtttcac cgattaattt tcctaattgc gtgaagatgg
     7981 tttttacttc agcaagaagc tcgtcagcca tccatgtcct tacaatgcgt cttccagtaa
     8041 tcggcaaaaa aattcctgct gaaacctgtg atgcaacagt gttgttgaac tcatcaataa
     8101 taactacatt tttttcttgc aataaaagat gccaggcaag caaactacct gctaaaccat
     8161 ttcctactat gatataatca gtttgcatga gagaaggtta gtttttgtta tagaagtact
     8221 tacgtccaga aagaattagg agttaagtgc taattatatt cagcaacgaa tactgtttta
     8281 ttcatctttc ttctttttgc gttttttctt gtcgtctttt ttgccggaat catctttagc
     8341 atcatcctgt tttttatctt ccttagtata ggcaaccaat gcaagttgag ttccttgttg
     8401 gctgattgca attctgtaaa aatttccaac agatttactg aagtctgcta gctcagtcca
     8461 tccattatca ggcttgtttc tgtcgaacat caataatttt ccttcgtaac caataagtag
     8521 tttaccatct tttgtccata cataatcttc aacacctttt ttaagttggc atacacttcc
     8581 aatcaatcct gtgttaattg aaaatgacat cagcatatag ccattggtgt ctgctttgtc
     8641 aacataactt atttcattat caccgtctgg aatgactgca atacaacgac caatattttt
     8701 tgccaatgga ataaattgtt cactcggcat ttcgtaaata tttaattcca ttacattgtt
     8761 gtttaagtct gcaacagcat aggtgctgtc atttgcaatg cagtaatagg caacagaatc
     8821 agatacacca agaagttgaa actcatttgt gccatccatc agcactttat aaaaccgctg
     8881 acctttatct gcatcaactc tcacaatagt aaatcctaaa tcatcagcag taacccgagg
     8941 agaaaattca ctttcaggag tttctgtcag ccttgtgttt gtgctatctt caagactata
     9001 aacatagatg tcgctctgca aagtatcgta ataggctaca taataaactt tactgccgtc
     9061 tttagagaac gagggctgat tgtcgtagcc cggacgatgt gtaatgttct gcggagctgt
     9121 aaaaaaatat tgcccttcgc ggttcatgcc aaagtttgtc agaaaaatat ctgttgacat
     9181 ttgttgtgca gatgttgtca gtactgagaa tattgcaagt actgataaat agaatcgtgt
     9241 catttatctt gagttttaat gatttttaat atggtgtcgc gtgatgctac ggtttttgtt
     9301 gttatatcta aactatcaat ttctgtttta ccgtgcatgt aagcacggag gcgaatttcc
     9361 gatttttcgt ctaaaaataa aatttttgca gaatcgggca caaaaccaat aaaaatattg
     9421 cgcacaaggg ttttattaaa gatgcccatt ctttttacaa ctttataggt agcttcatta
     9481 tctttttgtt taattaagcc ttgctcttta gagaggtaat agttcttgtc aaggtgcatt
     9541 tcatgtgcat tttctgtgtt cagatagccc atactcagaa tagtgagcat tttagatggt
     9601 gctatgataa acagtggtaa actcagcata aaaagaccaa agtataattt taatattagc
     9661 ggaaaatttc gtctgacaat gagcgtaaac ggtatgatgc cactacaaat tattgcagca
     9721 aacagcatga ctactctttc ttcatggact gaatttctat aaatcaaata cagcgtaatg
     9781 aaaattatat gcagtgcaat ggcaatataa ctaaaaaaaa cagggatgcg tggacttacc
     9841 atgagtaaaa tcaatgacac tacaaagcat gatattatgg taatgagtag aaaattcagc
     9901 agcgcaacca ttcaataaat attaagccaa aaatagccaa actacagggt gtttgtttaa
     9961 ctcctgtctt tgttttttcc agtcagaaat tcgctgtgtt ttaatcattt cattttcaca
    10021 ctgcagtgca gaggcaatgc aaagacgcgt tgaaggttgc agattattta gtaaatcatc
    10081 aaacaaggca ttgttgcgat agggtgtttc aataaagatt tgtgtttgtc tgcgttgtgc
    10141 agaatctttc tctgcttttt ttattgcagc aaaacgttct gttttttctt ttggcagata
    10201 gccattaaaa gcaaactgtt gcccattgag tcctgaagcc attaatgcta aaagtataga
    10261 tgatggacct gtaagcggaa ctacttttat ttgtagttta tgtgcttcgg caacaattaa
    10321 tgcacccggg tcggcaacag cagggcagcc tgcttcgcta agcaggccaa cattttttcc
    10381 ggaaagcaga ttgctgaaaa acgatgcttc aaaaacattg ccttcatgtt tattgagttc
    10441 atgaatggtg agctgctgtt gtggtgttgg gatgtgacaa tgtttaagaa actgtcttgc
    10501 agattttgaa ttctctacta taaactcatc caatgaatga attatttcaa ttacagtttg
    10561 tggcaaataa tctattgcct gcatactgcc aattgcagta ggaataaggt ataaaattcc
    10621 ttttgttttt ttgtcagaca acttcggatt gagcataagc aaactttttc aaaatgcgtt
    10681 ggcaggcatc gtcaagtaag ctatacacat tctcaaaatc actttgatcg ccatagtagg
    10741 gatcgggaac aatctttttg cccagacctg catagtctaa tataagatga atgcgttttg
    10801 cctctgctgc attattagca tattccatca ggttttcgaa gtttttgtta tccattgcaa
    10861 agatgtaatc aaaattttta atgtcatctt tgctaaaact acgtgccctt tgagcagtaa
    10921 ggtcaatgcc atatttggca gcaactgcta ttgagcgttt atcaggtaat tctcctgtat
    10981 gatagtttat caaacctgcc gaatcaacct gtgcagcaat acctgcctga ttgagtttat
    11041 gttgcattat gccttcggca agtggtgagc ggcagatgtt gccgaggcaa accatcagaa
    11101 ttttcacctt aatttttgtg attaagattt attcgctttt gcagatcgct gatatagcct
    11161 ttgaattttt tatcagtctc catcaggttg ttcactgtgc ggcaggcatg tagaactgtt
    11221 gcatggtctt tgccaccaca atgtgcgcca attgctgaaa gcgatgattt tgtcagactc
    11281 ttagaaaaat acattgctaa ctgacgtgcc tgtactactt cacgcttgcg tgtttttgac
    11341 tttaatgttt ctaaaggcag ttcaaaataa tcacaaacta ttttttgaat atagtcaatg
    11401 gaaatttcat gcacagtatt tttcacaaac ttgtcaatca tttgtttggc aagttcgaga
    11461 gtgataggct ttttatttaa tgatgtctgt gcaatgatag aaatgagtgc cccttcaagt
    11521 tctctgatgt ttgtagtaat actataagca atgtattcaa caatttcagt tggcaattca
    11581 attccgtcat tatacatttt ccttttcagg atggcaatgc gtgtttctaa atctggcgac
    11641 tgcaagtcag cagataaacc ccatttaaaa cgtgatagta aacgttgttc tattccctgt
    11701 aagtcaacag gtgctttatc agatgtaaga attatctgtt tgttattctg atgcaggtga
    11761 ttaaagatgt ggaagaatac atcttgtgtt ctttctttgc ctgcaaggaa atgaatgtcg
    11821 tcaataatta aaacatccat catctgataa aaatgtacaa agtcgtttgg tgaattgttt
    11881 ttaactgcat ccacaaactg attcataaac ctgtcggcag gaacataaag tacagtctta
    11941 ttctggaaat tattttttat ttcaataccg atggcatgag caagatgtgt ttttcctaat
    12001 cctgttccac catataataa taatggatta aaggaagtac caccgggttt gttggcaact
    12061 gcataacctg cagatcttgc aagacggttg caatcacctt caataaagtt gtcgaaagaa
    12121 tagtttggat tcagttgtga gtcaacatca atctttttta gtcccggaac aatgaatgga
    12181 ttttttatag ctgtgtttaa gctaaccggc attgctacct gtggattctt taagttgcct
    12241 gtccctttag caggaatttt cactgcataa ggttttgagc tgttcatgtt attctccatc
    12301 agaatagagt actccaagcg accttcagca cctaattctt ttttaatgct cttgcgcaac
    12361 agttgtatat aatgctcttc gagccattca tatacaaact ggcttggtac ttctatagtc
    12421 agtacatgct tttctaattt aaccggtttg atgggttcaa accatgtttt aaaattttgt
    12481 gggcttacat tgtcgcgtat aagcttcaaa caattttccc acactttttc aacatttttc
    12541 tctgtctgtt tcaacactaa tcgtaatttt aaaagttaag tattggtttg aggtaaaatt
    12601 tggtttttaa agatagaagt aaattttcaa ttgtaagata aaattcaaaa attatttttt
    12661 ggtggttctc gttttttaat cgaggtgcaa atatgctttg aatattcttg aaaaaaaaat
    12721 taaattgctc ttgcataatt caaaactttt aacgtggtac tgtcggctgt caatttacgc
    12781 atttttttat caacaaaaaa atctattctg cctaatttta ttcctgccca acctacctga
    12841 gccactaaaa catctttatt gtcgcgattt ttatacacaa agggtttgtc tatgaatgta
    12901 tgtgtgtgac caccaattat taaatctatg ttgagcgatt tctttgcaag aacatcatca
    12961 cttacctttt tgtttggata tctatagcct aaatgtgaga gacaaataat taaatcgcac
    13021 ttctcattga attttaaatg atgcgcagtt tctgctgctt tttccaaggg gtcaagatat
    13081 tttgtttcac catacatttt cttgtcaacc aagccttcca gttcaatgcc aagtccgaaa
    13141 actccaatac gtacatcttc tttttcaaaa attttatatg gtattgtttt gccctccatt
    13201 gaagtgcctt tgaaatcgta attacaaatt atcagaggga aattggcatg tttcaactga
    13261 cttgatagac cgtcaacacc attgtcaaag tcgtgattgc caatagtacc ggcatcatat
    13321 ccaagttgcg acattaattt taattctacc tcgccaccaa ataaattaaa gtaaggtgtg
    13381 ccctgaaaaa tatcaccggc atcaagaaga agtacattgc gttctttatt tctgatatca
    13441 ttaataatag cagcacgttg tgctactccg ccaagtcccg gatattttgg atcgttagcc
    13501 ggaaatgggt ctatgtggct gtgaacatca ttggtatgga gtatagttat cttagtaact
    13561 cttcttttag ccaaagcctc aagtggtaat cctgtgagac tgagtccggc agcgcccact
    13621 gcaaatgttc ttaaaaagtc tcttctattt gtcataaata atcctccctt caattttagc
    13681 agataatttt tctcctcgtt tatttttttc ttccagatat tgaattagca ggtcgcgaag
    13741 ttttaatccg gtagagactt tcttttcagc atcagctaaa aaagaaagat tgtcaccacc
    13801 attggcaaga taatctgatg tagccacaac ataacttttt gtactgtcga atggcattcc
    13861 ttgaatttta gcttcggtta cggttgattc atgtacgcgc attgttaatc ctgaaacagg
    13921 cataccacct ttagcagcaa taaaattgat aagttttaaa gttgttgcac catttaatgt
    13981 aagcaaaacc atttcatttt caaaaggcat tagtgagtag atgttgccaa ttgtaatatc
    14041 tccttttggc aatgcagcac gcaaacctcc attattcaaa aagcagaaat ctattttaga
    14101 aaagtcttta gcatggaaat cgttttctac ttgttttaat gctgcatcag aagtgaaatt
    14161 gccaagtaaa ccttcggggg tttttttctg catgctcatt tcactcacaa taatcacacg
    14221 atccattatg gagtcaatag tgtgtttgta agatgagtaa gagtttaaga ttgaggaatc
    14281 aacttcagta aagcccggtt tattaagagg gtaggtagtg gtttctgttt ttactaagtg
    14341 tgcattttgt ttgcatgatg taaatacaat tgcagcagca agtaaaatat ttcggaaaga
    14401 catactggta aaggttctca aaattgaatg ctaaaagtaa tgaaccaaat tatacgaagt
    14461 agttaaaaaa aaatattttt tttcggagta gtatttttca atcctttttt tattttatgc
    14521 aattttcata gtgcaaaggt taaatagtag tttcattgct atgcaagtgc agaattgata
    14581 actttgcacc ggtataaaga actcaatatc attatttcat gcaccctcac tttaaattat
    14641 tacttgctgt atttgttttg tttagtataa gcagtcaggc acagcgcatt gacaaatctt
    14701 tccgtataaa agtttctgat aacagaaacc tgcagtttac ctcttatgaa tatttgttaa
    14761 aggatgacag catgaaaatc agtggtgttt ctgattatgg aaaatcgcgt gtagtatata
    14821 tagcaaagaa attgaaatca aaacaaaaga aagcaataag aaactttttg aaagatttcc
    14881 ctgttgactc gctgcagaaa gaatattttg atgattatgc aaacttcggt tatatcgcat
    14941 ccgatcattt tccgagagta attgagcttg aaataagaac agggttgaaa cagcatttca
    15001 gcaaagcttc aaatgcctat gtgagaagtt atgcaaggct gatagatttt ctcaatacac
    15061 tgattaatga tgatgtggtt aaaatcaatt atccggaatc tgattttaaa aaaacatttt
    15121 agtctatgag aacaaaaaca tccaaacagt taaaagttaa tgttgttacg ttaggttgta
    15181 gcaaaaacgt tgttgactcc gaagtgttga tgggtcagtt gcgtgccaat gaaatagagg
    15241 tagaacatga atcgaaaaga caatcagaaa ttgtaattat aaatacctgt ggatttattg
    15301 ataatgcaaa acaggagtca atagatacaa ttctacatta cgcaaaagag aaggagaaag
    15361 ggaaaattga aaaattgatt gtcaccggat gtctttctga aagatacaaa cccgatttag
    15421 aaaaagagat aaagggtatt gacaatattt ttggtacacg cgatttgcca ttgctgctaa
    15481 aaactttagg agcagattat aagcatgaat taataggaga gcggttggtt acaacacctc
    15541 gtcattttgc ctacatgaaa atttctgaag gttgcgaccg tccttgttca ttctgtgcca
    15601 ttccgcttat gcgaggcaat cacatttcaa aaccaattga agagttggtt catgaagcaa
    15661 gacacttagc tgcaaaaggc acaaaggaat tactgcttat agcacaagac tctacctttt
    15721 atggtttaga catttacaaa gagagaaaac ttgcaaacct gatggagcag ttatgctctg
    15781 ttgatggtat tgaatggatt cgcctgcact atgcatttcc ctctggattt ccgctcgatg
    15841 tgcttgatat aatgcaaaga gaaaataaaa tttgcaagta cttggatatt ccacttcaac
    15901 atgcaagtga caatatgtta aagagcatgc gtagaggaat tacacgcgaa aaaacaatta
    15961 ctttattaga cacagtgcgt aataaaattc cggatattgc cattcgtaca acatttattt
    16021 cgggttatcc gggtgagaca gagcaggatc atgaaacatt aattcgtttt cttgaagaaa
    16081 tgcgatttga gagagtaggt gtttttactt attcacacga agagaataca catgcttaca
    16141 cattgaatga tgatgttgat gaagccacta agcaacgcag attggacgag ttgatgcagg
    16201 tgcaacagaa tatttctgac agtatcaatc aaaagaaagt cggaaatgta ttcaagacac
    16261 ttttcgacag gaaggaaggg aactttttta ttggcagaac agaatacgat tcaccggagg
    16321 ttgataatga agttttagtg gatgctaatg aaaattatgt tcgtgttggt gattttgcca
    16381 atataaaaat cacttctgcc gaggcatttg atttatatgg aacactttgt taaacttttt
    16441 ttacaagagg ttgttttaaa tctgttaatt acaaaacaat gaaactgttg ctgattatgt
    16501 tcgcatttct ttttggtcct gcttcaactg ctacttcaat ttatgagttt aaagttgatg
    16561 gtctttcagg agggcagatt gacttctcaa aatataaagg gaagaaaatt ctgattgtaa
    16621 atacagcatc taaatgtggt tatacaccgc aatacagtga tttggaaaaa ctgtatgaga
    16681 aatataagga taaattggtt attgttggat ttcctgcaaa taattttctg tggcaggagc
    16741 ccggaagcaa caatgaaatt gctgagttct gtaagaaaaa ttatgatgta acttttccaa
    16801 tggcagcaaa agtatcggtt aagggcagag atatgcatcc gcttttccgt tggcttatag
    16861 aacaacctaa tgtagatttt acaggaggca taaaatggaa cttcgaaaaa ttcttaattg
    16921 atgaaaacgg aaaactgatt catcgttttc gctcaagtgt gttgcccaca agtcctgaaa
    16981 taactaagta cctttagtct ctttaaaata attcattcga ctgattccat tgcagtgaat
    17041 ctgtcattga actatcagca ggcattacaa tttttaatgc cggcccttta agtttcacct
    17101 gcggttgaag attgaatgta tctattggtg aaatccatgt tttggaaagc attctgttgc
    17161 tcaccgttct atagtaggaa gcaaggtaag acttcaagtt accttgtgca tcaaatgaat
    17221 aacgatagta ttttggtcgg gggattattg atgcaagaaa aatactttct gccaaattca
    17281 gttgtgaagg aagtttattg aaatagaatt gtgatgcttc ttttattcca taaattccgg
    17341 gaccccattc aatggcatta aggtaaattt caaacattct ttctttactc accaatcctt
    17401 tgttttctat tagccaaaca ataagcatct cctgtagctt acgcgaaatt gttttttcac
    17461 gagacaaaaa cacatttttt accaattgca tgctgatggt gcttccacca cgcacaaacc
    17521 tttttgcttt aatgttttca atcatgcttt ctttaaaggc atctggaata aaaccacgat
    17581 ggtagaaaaa agaaggatct tctgaagtga ggacggcata ttgcagatag tgactgatct
    17641 cattcagtgg agtaaaatca ctgttctcag gcccaatcag aaaacttcgt tgcaatctgt
    17701 caccatcata ggcatcaaac agaaatgtgt tgtttatacg tgagaaattc tcatcaccat
    17761 atttttcaat tgtcaatgca ttgcctgtta agtctgaatt aaatatcaga gaatcggggg
    17821 ctgacatgtc aagtgcaaag tttaaatcaa attttaatct gcctttagct ttgaagtttt
    17881 tgaagtgata aaaaattcct ttgggaagcg attcaaaaaa atcatctgca ctttcgtcaa
    17941 atgctaaaga aagtgcatag ctatgaggct gttttctgtc gaaggcagct tttgttttaa
    18001 aatggatttt attgatggta atgtttacag cgctatcggt ttcaattcgt tgattattga
    18061 atgagcaaat tccggaaaac gaaagtttat tgaaatcaac atcatcgttg gcaatacgcc
    18121 aataatttac actcaggcta tcaagttctg ccttaacctg aaatgttgta tcctttcttg
    18181 agggttgttt aatttgtagt gaaatagccg aataagcaat ctttggtttg ttgtcaacat
    18241 caaagaatgg aagccagttt tctcctgcca gttttttgat gtttagtgac atttcgtttt
    18301 tatcatcatc aatattaaag ccggcttgcc atttttctgt ttgtaaatgg tcgttgctgt
    18361 aagctataaa gcggcatttc cctctgcgat ttataatttc cggaaagtaa aagtttctgg
    18421 tttctttatt tctgatgatc gtaattttgc atcgttccaa atcaaaactt aagtcggaca
    18481 actctgtcaa cttattccaa agtgtaaata cttttgaagc ctgtgtactt tgtactgatg
    18541 ttatttctgt gttttcaggt tttgattttc taaaaagaaa ggagtagtta ctaattgaat
    18601 caccggaggc aattttaatt ttaatgccat gtgccgaaaa attggagaca ggatttttta
    18661 gacgaataag ctttaataat gaaagctgca ccataaaagt atctgtatgc aacagtgtgt
    18721 catttaatgg tgaaaccaaa ctgatgtttt caatttcgat gaccctgagt ccattgaatt
    18781 gaatttttcc gatgttgatg ttgccaccgg ttttattgtg gaatgaagta caaaaagaat
    18841 ttactttatt gcgtacaata gattcccgaa gtagcattac cgcgccaaat accacaacga
    18901 aaactgtaag aataattatg aaatattttt ttatcaaagc aacatgaaat cagcaatatt
    18961 attcctgact aaaattataa ataaaaacga tttcagtaaa ttattttgcc aaatcaagca
    19021 tgccatagcc ttcatgggtc atcactccaa tgccattatg ccaaacataa cggtgaacat
    19081 cgggatgtgc atgcagtgta aaaggaacta aatatccaat catattcttg ccttcagcac
    19141 ttctgtaaac acgggcaaat tttttatttg ctgctgcagc tttttgcata tactctgcat
    19201 caggctgtac gtcaaattct gcataagagc taatagttgc ttcaggcaca cctgactttt
    19261 ccatggcatc aagtgttgtg ttaaataata tgtcggaaaa gttatgtgat gtaggatcaa
    19321 tctgctcagt gctgcgtgct gcatctttaa taggatcaaa aagtactaaa ggagatatac
    19381 agagatagcg catctgcgtg gtaaactcag ggtcagaagt aatttctttt ccttttggaa
    19441 gcagattcat tctgccaatg ggcataaaat ttttctcaaa tattctttct ttactcacca
    19501 atcctttgtt ttctattagc caaacaataa gcatctcctg tagcttacgc gaaattgttt
    19561 tttcacgaga caaaaacaca ttttttacca attgcatgct gatggtgctt ccaccacgca
    19621 caaacctttt tgctttaatg ttttcaatca tgctttcttt aaaggcatct ggaataaaac
    19681 cacgatggta gaaaaaagaa ggatcttctg aagtgaggac ggcatattgc agatagtgac
    19741 tgatctcatt cagtggagta aaatcactgt tctcaggccc aatcagaaaa cttcgttgca
    19801 atctgtcacc atcataggca tcaaacagaa atgtgttgtt tatacgtgag aaattctcat
    19861 caccatattt ttcaattgtc aatgcattgc ctgttaagtc tgaattaaat atcagagaat
    19921 cgggggctga catgtcaagt gcaaagttta aatcaaattt taatctgcct ttagctttga
    19981 agtttttgaa gtgataaaaa attcctttgg gaagcgattc aaaaaaatca tctgcacttt
    20041 cgtcaaatgc taaagaaagt gcatagctat gaggctgttt tctgtcgaag gcagcttttg
    20101 ttttaaaatg gattttattg atggtaatgt ttacagcgct atcggtttca attcgttgat
    20161 tattgaatga gcaaattccg gaaaacgaaa gtttattgaa atcaacatca tcgttggcaa
    20221 tacgccaata atttacactc aggctatcaa gttctgcctt aacctgaaat gttgtatcct
    20281 ttcttgaggg ttgtttaatt tgtagtgaaa tagccgaata agcaatcttt ggtttgttgt
    20341 caacatcaaa gaatggaagc cagttttctc ctgccagttt tttgatgttt agtgacattt
    20401 cgtttttatc atcatcaata ttaaagccgg cttgccattt ttctgtttgt aaatggtcgt
    20461 tgctgtaagc tataaagcgg catttccctc tgcgatttat aatttccgga aagtaaaagt
    20521 ttctggtttc tttatttctg atgatcgtaa ttttgcatcg ttccaaatca aaacttaagt
    20581 cggacaactc tgtcaactta ttccaaagtg taaatacttt tgaagcctgt gtactttgta
    20641 ctgatgttat ttctgtgttt tcaggttttg attttctaaa aagaaaggag tagttactaa
    20701 ttgaatcacc ggaggcaatt ttaattttaa tgccatgtgc cgaaaaattg gagacaggat
    20761 tttttagacg aataagcttt aataatgaaa gctgcaccat aaaagtatct gtatgcaaca
    20821 gtgtgtcatt taatggtgaa accaaactga tgttttcaat ttcgatgacc ctgagtccat
    20881 tgaattgaat ttttccgatg ttgatgttgc caccggtttt attgtggaat gaagtacaaa
    20941 aagaatttac tttattgcgt acaatagatt cccgaagtag cattaccgcg ccaaatacca
    21001 caacgaaaac tgtaagaata attatgaaat atttttttat caaagcaaca tgaaatcagc
    21061 aatattattc ctgactaaaa ttataaataa aaacgatttc agtaaattat tttgccaaat
    21121 caagcatgcc atagccttca tgggtcatca ctccaatgcc attatgccaa acataacggt
    21181 gaacatcggg atgtgcatgc agtgtaaaag gaactaaata tccaatcata ttcttgcctt
    21241 cagcacttct gtaaacacgg gcaaattttt tatttgctgc tgcagctttt tgcatatact
    21301 ctgcatcagg ctgtacgtca aattctgcat aagagctaat agttgcttca ggcacacctg
    21361 acttttccat ggcatcaagt gttgtgttaa ataatatgtc ggaaaagtta tgtgatgtag
    21421 gatcaatctg ctcagtgctg cgtgctgcat ctttaatagg atcaaaaagt actaaaggag
    21481 atatacagag atagcgcatc tgcgtggtaa actcagggtc agaagtaatt tcttttcctt
    21541 ttggaagcag attcattctg ccaatgggca taaaattttt ctcaaatatt ctgttgctga
    21601 cttttgttat taaatcatca ttactgcccg atatcactaa agtaatttta gaagataaaa
    21661 acttcataaa tccattttga atttttgagg ttccttttaa tgacgaaaaa tttagctgtt
    21721 tcatattgcc accaacttcg tgaacaacag tttttaaact atcgtaaatt agtttttgat
    21781 gatgcaatgg aatactattg ccggaagtat tgtcgcgggt gaatgaaatt tttactcgca
    21841 taattgaaaa atgttaatca gtttagttag tgaaaatagt ttggttatgt caatgaaaat
    21901 tagaattgtt ccaaaagtaa aaaaataatt cacaaattca taaataaaaa tgttaaaaat
    21961 ttagggtaga aataacaggg tagtggtctg ataaatgcac taatggtcga atatattcta
    22021 tggttttaat gcttttactg tgtaaaatat agtcaattct aagtcctgga aacatacctg
    22081 cataagttga ggacagtcct tttccacagc taagaaaact atctgtcaag ccattgctga
    22141 tttggttgta ggcgtaagat gcaggtgtgt cgttaaaatc accggcaaca attactttat
    22201 acgggcaagc acctatatga gtggtaacct tgcaaacctg atgcgctctt tttataaacg
    22261 cctttttcaa acgttgaaca atttgcttag agcctttcat tttctgagtg ttgtctttat
    22321 tgttaaactc actcacaaaa atatagtcgt tttttgccaa tcgtactgat tgcaggtgta
    22381 tgttataaac tctcactgta tcgtttccaa tcagcaaatc ggtagcaatg catccgttgg
    22441 cagattcttt atctgtaaat actgtttctt tattcagtat tggatatttg ctgaatgtag
    22501 caatgcccca ttcatcagaa tcgcgttcta tgattggcag catcgtgtgt acattggata
    22561 ttcctaacaa tgattttaat gaatcggtgt tattaaatgg cattaactta ctcgtataat
    22621 attcctgaaa acagtaaata cctgcatcac tttttttcag aaagttaaaa atattacttc
    22681 ttgtttgcag attatgcgtc cagttgtaaa gatcaaaaac acgcacattg aatgaaagaa
    22741 cttttatcgt attttctcct gttgcttttt ggtttgatga agagtaattc caatgtgcaa
    22801 caaatgttgg taagcctgct attaatggaa ttaaaatcag cagcatcatt tttttatgtc
    22861 tgaatgataa gaccaacatc cagataaatg ctaccaaata gatataaata aatatcagac
    22921 cgaatagtgc aagaaaccaa atttttgcag gactaatata tgccgctgca acagccataa
    22981 aagttacaac agcaatgaaa atggttatta gccatagcaa caagttaaga gcagacttga
    23041 gcatcaatcc tgatttttac ttgctttaaa aagtatttct ttttcttcgt tggtgaggct
    23101 tgcatatcct gaacggctta ttttatccag aattttatca attgcttcct gattgtttgc
    23161 ttttttgttg tgatacgatt catcagaaga atctttgcga taaacaacct taattgatgg
    23221 ttttttcttt tgagtaattc tatcaatcat tcgttgaagc catccggtta aatcacgtcc
    23281 tttttttaat tgcttgatat agataaaacc aaagatggca ccacctatgt gagcaatgtt
    23341 tccgcctgca ttgttgcctg taatacttaa caggtcaatt acaaaaagtg caatggcgat
    23401 atatttcagt tttacccgcc actgtggcag aaacagacct acttcgtaat ccggtaacag
    23461 tgttgcaatg gcaacagtaa cagcaagcac acttgcagag gcacctaagg caactgcaat
    23521 atcaacttcg cgactgaata gtggaaacag attgtaaaat acaataaaca acacagagcc
    23581 acataagcca ccaacaagat aaagaggtaa taattttttt tcaccaaggt attctgtaaa
    23641 aagacgaccc ataaaaaaca gccataacat attaaacaga atatgaagaa agttttcgtg
    23701 gtagaaacaa taggtgatta atgtccatgg acgatacagc aatgacttga gagaagaagg
    23761 aacagcaagc cagtcggtta aaaagccgct tactgtgcca tcaggaaaat taaaaagtct
    23821 aagcacaaca tcgcctatgt ttacaaacaa ataaataatt gcattgacta caattatctt
    23881 ttgcagcata tcgagcgact tgtaattact tttcacattg tgaagcattt tcagaaaagt
    23941 tgtgaccggt tatttttctg ccaatagcga agtagtaaat atccgaaaag catgccacca
    24001 agatgtgcaa aatgtgcaac attatctgtg gggttgcttt gccatgcaga atacaactct
    24061 atgccaccaa taatgagtac taaatatttt gcttttagag ggataaaaaa gtaaagataa
    24121 attaaagtgt ttgggaaaag aattccaaat gccatgagca caccataaac tgcacctgat
    24181 gcaccaacag taggtatatt ggttttaaaa cgaatatatt catcaatagc ctgacgcgcc
    24241 tgttcaataa tcattggact gtcgccatgt gcataccagt ttgatttaat gttttccact
    24301 gccatggcat aatcggcatt cagatattga tagggaatat ggtctctgac aaacacgcta
    24361 aaagcatcag cacccggagc attaaaaaaa gcatcggctt tagcctgtaa cgaatgcaat
    24421 tcaaaatatc caacagctgt gtgaatgaat gctgcaccaa gtcctgttac caagaaaaaa
    24481 atcagaaatc tttttggtcc ccacaaattt tcaagaatgc cgccaaacat ccagaaggca
    24541 aacatgttca gtaaaatgtg tgagaaactt ccatgcagaa acatgtaaga aaagtactgc
    24601 caccaatgaa aatgctcaga agacggaaga taaagtccgg cataagcatt aagatcaaac
    24661 ccgattttat taagtgccaa atcagcaaaa aatgccaatg cactgacgat cataatattt
    24721 ttaattacat cgggtaaaag tttgaaactt gaaggtcgga attcctgcat aaaaaacgat
    24781 tacaaatttt ggcaatccat tgttaaacgg tacgaagata ggtttccgta tttaatcata
    24841 tcaaacagat gatattttct ttggtttaaa caagcattaa cctttttcaa acttgcggtc
    24901 aatttcattt ttttctaatt taaaaattat tgccttaccg ctcttggtaa agtagggcat
    24961 ttcgcattgc atcagatcat taaaaagagc agacatctct cttgcagaaa gcactttacc
    25021 actttttaga catgcactgc gggcaatagc aatggcaaca cgctccattg gattgtaggc
    25081 cgaagcttcg ccactgataa actcatcaat aagcatttca ataattttct tttctgttcc
    25141 ggtttctaga taggagggaa cagcattaag tacaaaagta ttctgaccga atggctgtaa
    25201 gtcaaaaccc gaacggttga ggtcttcaag aagcgatgaa acagtagcag cctgtctgtt
    25261 gtcgagttct atctgttgcg gaaacaaact ttgttgcata acagtaggtg tgcctttcat
    25321 ttgtaaaagt gttttttcaa acacaatttt ttcatgtgca agttgctggt caataagcca
    25381 catttcgtct ggcattgcta ctgccacata actgttgaac agttgcataa atgcagatgc
    25441 attattttct tcaataggag ctattggtag ttttgtctgt tgtacatcgg gcgtacttgc
    25501 aatctcatat agtttttccc actgcccatt ttgttgctga gtaggtttat ttatctcagt
    25561 acttttaaat ggattgtaat tggtattcac tttcacctga ggctgtacag gaatagtgtt
    25621 taactgcgat aacggcacat taaaagcttg ttcctgatta aagtctaacg taggtgcaat
    25681 actgaatctg cctaaagcac gtttcacaga agagcgcaat atggcataaa ccgatttttc
    25741 atcttcaagt tttacttctg tttttgtcgg gtgaatgttg acatcaatac gcgaagggtc
    25801 aacttcaaaa aacaaaaagt acgatgcaaa agtgccttga ggtaacaact cattgaatgc
    25861 aatggtgaca gcatgattca gataggcatc tttaacaaag cgtttgttaa tgaaaaaata
    25921 ctgttcacct ctgaccttct tggcaaactc aggtttacct acaaatcctt taatatttaa
    25981 aatggttgtg tcttcttcta caggcaccaa tctttcatta taagttgctc caaaaacctg
    26041 acatatacgt tgccgttgat gtgttgccgg caggttgtaa acttccatgc cattatgctg
    26101 aagactaaat ccaatttcag gtcgcggtaa agccacccgc agaaactcat ccatgacatg
    26161 acgtgcttct accggtgttg ctttcagaaa atttcttctt gcaggaacat taaagaaaat
    26221 attttttacg gtaataattg tgccggactg catcacacat ggctcctgaa ttttaaactc
    26281 agagccttca atttctatgc gtgttccggt ttcatcttct tctcttttag tctttaattc
    26341 aacctgacag acagcagcta cagatgccat tgcttctcct ctgaacccca tcgttctgat
    26401 ggcatataag tcatcagcct tgcgcaattt agatgttgca taacgctcga aacaaagacg
    26461 cgcatcagtc tcactcatgc cacagccatt gtctgtaatc cgtatcaggt tttttccacc
    26521 atcgttaata cttacatcaa tgcgtgttgc gcctgcgtca acagcattct ccatcagttc
    26581 tttaataact gatgatggtc ttaaaataac ttcgcccgct gcaatctgat tggcaacagt
    26641 atcggacaac aattgtatta catctgccat gcggcaaagt taatcaatgg caggtcaata
    26701 ttaaagaatt acagtgagta gcttaaagat tattcatgga aaatttggaa ggcagtgtac
    26761 tatattttta gctgccatta tcaaaaagta cacttataca tcggtattgg taaaaaaccc
    26821 atctgatact ctgtttttac agtgtttgtt ttggcgctat actgctgatt ccagatgttt
    26881 tgtgtgttga agacattatt caggtcaatg gcaatttcat gagttagtct ttttctgttt
    26941 atcctgtaac ctattttcag gtcggtgcgg aagtaatctt ttctacgatg ctcaaatgca
    27001 tggtcttcat cgtattctgt ttctcctgta atctgcgatg ctgcaaagtc aataggaata
    27061 tatctgcgcc caccggcata ggttgtttta atatttattg aaatggtgtt cttttttctg
    27121 attgtccatt cttttccccc caataaattg aaaacaaaat tgccattaaa tgcagtgctt
    27181 cgttcaacgc catcagag
//
LOCUS       DAOOOL010000016        27058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_137_length_27058_cov_179.091731, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000016 DAOOOL010000000
VERSION     DAOOOL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27058
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_27058_cov_179.091731"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PKU82_02590"
     CDS             complement(<1..173)
                     /locus_tag="PKU82_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOZ81792.1"
                     /translation="MKSEIHPKNYRMVVFKDISCDYAFLTRSCVDTKDTITWEDGNEY
                     PLVKLEISNMSHP"
     gene            457..2334
                     /locus_tag="PKU82_02595"
     CDS             457..2334
                     /locus_tag="PKU82_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOZ81793.1"
                     /translation="MFLFERTAPRWVVLVIDLILCTLSFYLAYDLRFNFTIPDVEIQL
                     LPTAFATVLSVRLVSFLISRTHTGIIRYTSYKDSQRIFLTLLSGTVLLCIFNLISFRI
                     YGRYFTPYSILMIDFFLSMLALASMRVIVKSAHVEWTRKKGNKTDVLIMGAGEEGMVV
                     KHALEQDAGINFKVLGFIDDKPKLRKRKLEGIPVFYTKTDLEDLLRANKISSLIIADK
                     DVDTKRINELIEICVKYDTKVQRAPRAGNWINGKLSFSQIKEVNIEDLLERDPINLDV
                     KEIRNQLNGKTILITGAAGSIGSEIARQVILYNPAKVVLLDIAESPLYELELSIQEIK
                     HAGNCESVIADIRNIDRMRRVFDYFKPEIVFHAAAYKHVPVMEVNPSEAILTNVKGTK
                     ILADLSVEFGVQKFVMISTDKAVNPTNVMGASKRVAEIYIQSLSEISETQFITTRFGN
                     VLGSAGSVIPRFKKQIENGMPVTVTDENITRFFMTIPEACQLVLEACKMGKGGEIFIF
                     DMGKPVKIAELAKKMIRLSGLEPEKDIKIQYTGLRPGEKLFEELLNKQENTIATHHPK
                     ILIAKVRKYKFDQIASDIENLTNLFKTQNNMALVSKMKEIVPEYISNNSEFEKLDRRE
                     I"
     gene            2522..4426
                     /locus_tag="PKU82_02600"
     CDS             2522..4426
                     /locus_tag="PKU82_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOZ81794.1"
                     /translation="MLVLNNISFDFGGRYLYHDAFWHIKPNQKIGLIGANGTGKSTLL
                     RIINGEYTLTGGEITGRKDLTVGFLNQDLLSYESDKSILDVALEVFERENILHQKIEK
                     ILKQLETEHGEELLNELFFLQSEFESLDGYNITHRAESILEGLGFSTIDLQRPLKEFS
                     GGWRMRVMLARMLLKKPSLLMLDEPTNHLDLPSIQWLEKYLQDYDGTFILVSHDRYFL
                     DRTINMIAEVANEKINLYPGNFTNYLEQKTMRDELQQRKFENQEKYIKEQQKLIDRFK
                     AKASKAKMAQSRMKMLDRMEKVEAVSGPASTMRLSLQVETQPGKVVFEGHHLNKNYGD
                     LDIFKNSHFTIERGDKIALIGANGKGKSTLLRMIAGTEKADGEIKEGYNVKTSFYAQH
                     QLESLNIENDLMRELQIFAPLETDQRIRSILGAFLFNRDDVFKKIRVLSGGEKARVAL
                     AKLILSKANFLLLDEPTNHLDMQSVEILTDVLLDYEGSFVVVSHDRFFVEQIANKIWF
                     IENNQLKEYPGTYEEYEHWAERKKSEKRTEEKQVVAKPVAPKVQAEPDQKKQLLNLEK
                     EVKKLEAELAAAKKQRNDLMEQLGNESNYNDTAKIKLLQKEFDESEKQYNKIHSLWED
                     SYMQWMEMSE"
     gene            4434..5729
                     /locus_tag="PKU82_02605"
     CDS             4434..5729
                     /locus_tag="PKU82_02605"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOZ81795.1"
                     /translation="MKHFIFYLILFFLWSNSNVFGQEKQEYFKDNYFRYDNFTYRSNI
                     KTIQLENAQFPMSEPVIELNSGAQLLLQFDDLEGDYKDYWYTIIHCDAQWKPSDITRS
                     TYLFSFYEDRIADYAFSFNTLQPYTHYSLKFPNEQIKPLISGNYLLKVYLNNDPDSVA
                     FTRRFLVYENLIDVDATVHAATLAEYRQQKQEVDFSLSTTRYTIANPFGDLKVKILQN
                     FRWDNAVENLKPIFIKDNLLEYNYDEENTFNGSNEFRRFDIRSLKYETESVERIDHFD
                     TGTNVLLFPDKVRYFSRYTTDYDIDGKFEIKNYSGSHPERDADYATVRFRLEYKDEVK
                     DGNIYIFGALTDWGFTSQNMLRYDEDLKAYTATLFLKQGYYNYMYVFLKDGTSFGDES
                     WLEGNHYETENNYWILVYHRPQTERYDKLVACRKFASNR"
     gene            complement(5741..5839)
                     /gene="ffs"
                     /locus_tag="PKU82_02610"
     ncRNA           complement(5741..5839)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKU82_02610"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5916..6434
                     /locus_tag="PKU82_02615"
     CDS             5916..6434
                     /locus_tag="PKU82_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOZ81796.1"
                     /translation="MFRYGLHYGGILGASSFAMFLIIYLTGNNPLGNASWLGAWIPAV
                     VIAVAVKSYRTHEGEGFISYGEGFRTGLITALFGGILSALLIYIFCTLFDSTLVERFK
                     TDSLAQLELMEGQFKSMLSDKMYDQAVDAYNNISLQSIAMNDFYNKLLSGIILSLIIA
                     AVYKRNKPQILE"
     gene            6454..7419
                     /locus_tag="PKU82_02620"
     CDS             6454..7419
                     /locus_tag="PKU82_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ81797.1"
                     /translation="MDISVVIPLFNEDESLPELAAWIDRVMKQHKFSYEVIMVDDGSK
                     DHSWQVIEDLNKQIPEIKGVKFKRNYGKSAALHKGFEMAQGHVVITMDADLQDSPDEI
                     PGLYKMIVEDEYDLVSGWKKKRHDPISKTIPSKFFNGVTRMVSGIDNLHDFNCGLKAY
                     HHNVIKNIEIYGEMHRYIPVIAKWVGFTKIGEKVVEHRARKFGTTKFGLERFVNGFLD
                     LLSITFVSRFGKRPMHLFGTLGSLMFILGLIGACYLGVSKLYCVYNDIPATRLTQRPS
                     FYLLLTSMILGTQLFLAGFLAEMIARNDKDRNRYIVEKEISNTTT"
     gene            7438..9378
                     /locus_tag="PKU82_02625"
     CDS             7438..9378
                     /locus_tag="PKU82_02625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ81798.1"
                     /translation="MKLSVVIVNYNVKYFLEQCLISVGKAISNIDAEVFVVDNNSVDG
                     SCQMVRDKFPFVNVIENKINTGFSKANNQAIVQSKGEYLLLLNPDTVVEEDTFKKVVD
                     FMDNHPQAGGLGVHMIDGNGKFLPESKRGLPTPAVAFYKIFGLSALFPKSKLFGQYHL
                     GFLDKNKTNEVDVLSGAFMLLRKSVLDKIGLLDETFFMYGEDIDLSYRITKAGYKNYY
                     FPETKIIHYKGESTKKSSVNYVVVFYKAMSIFAQKHFSQSNAAFFSFLINIAIYLRAG
                     IALMNRLFSILLLPIIDAAFIYGGYTIIKTYWELYVKQLHYPEHYMLLVVPGYVLVWI
                     FAAYLNGGYDKPLRISRVVRGILSGTIFILVVYALLNETYRFSRALILMNTAWACLIT
                     ALIRYTLNAAGIKGFALAADVKKKLIVVADAVDAKRILSLLQLSGSNTTFVGYVDSGV
                     TQDGELKNFRLGTLTQLNEIITIYQVDEVIFSSHSLSSQQIIENMGALRNRQVEYKIA
                     PPESLFIIGSNSINDNGDLYLVDVNTLHSPSNRRKKRFFDIFSAFGILFCYPFLWLLF
                     KKNQNVFVNSIAVLFGKKTWVATNIKSLTSTHAKVGVYTPSDGLNTPSDNEYFRDRLN
                     VLYTRDYNMINDWNIVRRNIFK"
     gene            complement(9386..10474)
                     /locus_tag="PKU82_02630"
     CDS             complement(9386..10474)
                     /locus_tag="PKU82_02630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ81799.1"
                     /translation="MIQLKSQEVINAPGRNSSVDIFRSFAILSVVIYHYNRQLPFGYL
                     GVDLFFVISGLLIGNILIGYLKKGEKINFFKFIIQRGFKIWPSYFSYLFIGSIIANVL
                     YHTLAPDMIIPADKYLRYILFYVNYTGNEVWSFGHLWSLCVEEHFYIMLPLLLILIQR
                     FFPDKKMLVTAILLVITAGVIFKFLVMAFTNSKDTYSATHNRIDALGWGVMLSIILNF
                     YPKLIVKQSTQITMLIAGIILFSLLLYTYSAYEWITFRKCVFHSAIPFCFFLMLAATY
                     HWNMSGLNWLRFIAYYSYNWYLWHPLWVTWFNKKIGTGLMAHLVFLIFTFLTAMAFTI
                     FIEEFFMGKRNKFISKWFGERKIQVKTL"
     gene            complement(10482..11243)
                     /locus_tag="PKU82_02635"
     CDS             complement(10482..11243)
                     /locus_tag="PKU82_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOZ81800.1"
                     /translation="MKELIRFLKSRIFWINALVIIIVIIMSIGLVYKWLDSFTDHGNS
                     VSVPDLKGMNIKKVDDFLKTKNLSFKIADSSVYLLDQKPGTIVEQDPQPNEKVKQGRT
                     IYLTITRSSAPMVKVPALKDVSLRQAEAILAASGLRMGEQIFKPDLAKNAVLSMMING
                     KDLKAGTDVPKGSAIDLIVGDGLGNTVVTVPSLIGLTYDEALFVLKGSSLNIGSLFFD
                     GVIKDTLNAKIYDQNPAPDNNTINQGEAIDLYLRP"
     gene            complement(11248..13941)
                     /locus_tag="PKU82_02640"
     CDS             complement(11248..13941)
                     /locus_tag="PKU82_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOZ81801.1"
                     /translation="MNKLKIGILFGGSSREREVSFAGGRTVYDNLDKTLFEPIPLFVD
                     SFGNLLILDWQFIYKGSIRDFYPPSEFLPETTRGFQIYAENLGTLTHQEQIRLTMKLG
                     TRIEFAQLHDVIDFAFLCLHGSDGEDGRIQGMLEYYRIPYSGSGILSSAIGMNKVIQK
                     ELMKKSGFNVPEVAVINRIEWLASPNRKSFIKNLKSVGFPCVVKAANQGSSIGISVLK
                     NNDVEEFIQAVNKSFFIREITRTEWNSMTFDTKTEWIKKVSDIREGIGLPAKINDRTI
                     YHPEELLGVLIQLFGEMEDVVSIAAIEAETEVIIESFISGKEFSCIVITNENEKPVAL
                     PPTEIRKGNELFDYRAKYLPGLSRKVTPIHLPEVQIETIRRECERLFTSLNFSVYARI
                     DGFITENGTIFLNDPNTTSGMMPSSFFFHQAAEIGLNPSQFISYIISTSLQARLLEMK
                     SFRTVPQLIKNLHGLLSKKAKSQSEKIKVGVIMGGYSSERHISVESGRNVYEKLSSTE
                     KYEPIPYFLAGNEQSYSLYRIPINLMLKDNADDIREKILNYNQHPVIKKIIDQCGQVT
                     QTFANAANINPPEKTSFEELAQQVKIVFIALHGRPGEDGELQLQLNKVGLPFNGSRAE
                     SSKITIDKFQTNELLAQNGFKVAQHYKLTEEEWRNNPSAIEEKIQKEFGFPIIAKPVD
                     DGCSSAVKIIRNENQLHAYAELIFREDETLNTAAAELLKIKDKEEFPRKRYFLIETLI
                     SGENTKHFLEITGGMLTSYDEAGNIHYEIFEPSETLHEGDVLSLEEKFLAGEGQNITP
                     ARYSKHAMENRLISDIIRKELLRAARLLNIEGYARIDAFVRITIDNKVEVVFIEVNSL
                     PGMTPATCIFHQAAINQYKPYEFIEKILEFGFKRNQLSLTK"
     gene            complement(14128..14892)
                     /locus_tag="PKU82_02645"
     CDS             complement(14128..14892)
                     /locus_tag="PKU82_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOZ81802.1"
                     /translation="MNHHELKLYLDDLFLRYNRSDFIADDPISIPHKFSDSGDIEISA
                     FLTSLISWGQRKTILKNAQTLMEMMDHTPKSFIINASNNDYIPFKKFVHRTYNGEDCI
                     YIIKAIKNIYAEYGSLGNCLTSFVSNNPMPVAMSLFRQKLFSQPCQPRTTKHLGDPLK
                     NSSCKRFNMFLRWMIRKDRAGVDFGIWEISPSLLYCPLDVHTGRTARLLGLLKRQQDD
                     WKAVEELTINLRQFDKSDPAKYDFALFGAGVNGLLK"
     gene            complement(14905..16224)
                     /gene="der"
                     /locus_tag="PKU82_02650"
     CDS             complement(14905..16224)
                     /gene="der"
                     /locus_tag="PKU82_02650"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOZ81803.1"
                     /translation="MSNIIAIVGRPNVGKSTLFNRLTESMDAIVHDESGVTRDRHYGT
                     GEWTGRSFSVIDTGGYVSGSEDIFESEIRKQVKLAIEEADIILFVTDAREGLNDFDED
                     IAQLLRNSNKKTFLVVNKVDSPKQLNDAVEFYALGLGDYYCISSSNGSGTGELLDAVV
                     KVFKPDDEEEDELPRITIIGQPNVGKSSLMNMLTGVERSIVTPIAGTTRNSINIRYSN
                     FGFDFILVDTAGLRRKAKVKEDLEFYSVMRSIRAIEQSDVCLFMIDAEKGFTAQDANI
                     FHLIQTNHKGVVFLVNKWDLIDKENHSTKKFTEEIKAVIAPFTDVPIIFTSVLNKQRI
                     FKALETAIDVFKNRTQHIPTSQLNKKILPFIENYPPPSVKGKLINIKYITQVPGPYPR
                     FVFFSSNPQYIQTSYRRFLENKLRENFNLSGVPVAISFRSKDSGKEV"
     gene            complement(16234..17109)
                     /gene="era"
                     /locus_tag="PKU82_02655"
     CDS             complement(16234..17109)
                     /gene="era"
                     /locus_tag="PKU82_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOZ81804.1"
                     /translation="MHKAGFIAIVGKPNAGKSTLLNTLLGQDLSIVTHKAQTTRHRIK
                     GVITQPDYQLVFSDTPGIITPGYQLHQRMMTFVQSAFTDADVILLVYDATGNDMDAEK
                     FRTLLPAGKKLVVALNKIDLSNQEELEKIVQKIEQQLSPDAIVPISAKEKFGGEVLIK
                     TLLQFIPEAPPYFDKDTLTDRNERFFAAEIIREKIFLQYDKEIPYSVEVSIEEFKDTP
                     KLAKIKSYIFVERESQKAILLGKNGLAIKKLGTDSRKKIEEMTGKKVFLELTVKIRED
                     WRNNEQQLNKFGYNE"
     gene            complement(17173..17799)
                     /locus_tag="PKU82_02660"
     CDS             complement(17173..17799)
                     /locus_tag="PKU82_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOZ81805.1"
                     /translation="MYIPEYFRNTNFNQQVDFIKENSFGILITHSATEMFATHIPMTP
                     LVTNSNLVLHGHISKENPQCSTLINAGETLTIFSGPHCYISSSWYSHENVSTWNYQAI
                     HCYGKLKIQTELELLQSLIQLTDHYESKEEKPKFFEKIDPKVIRDNLIGIIGFEITVS
                     RIDAKDKLSQNRNKKDYENIVSHLCKRNDSMSDAIAEAMIKNADNANK"
     gene            complement(17817..20048)
                     /gene="purL"
                     /locus_tag="PKU82_02665"
     CDS             complement(17817..20048)
                     /gene="purL"
                     /locus_tag="PKU82_02665"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOZ81806.1"
                     /translation="MESLKETTVETAKKLGLLPEEFDKICSLMGRKPNFNELSIYSVM
                     WSEHCSYKNSITWLKTLPREGPHMLAKAGEENAGLVDIGDNLACAFKIESHNHPSAIE
                     PYQGAATGVGGINRDIFTMGARPIAQLNSLRFGNLNNERTKMLMRGVVKGIGDYGNAF
                     GVPTVGGEVYFEDCYNTNILVNAMSVGIVEHGKTVKAISEGEGNSVYIVGSATGKDGI
                     HGATFASGDLHEDSHEDLPSVQVGDPFQEKLLLEATLEVIATGAVVGMQDMGAAGIIC
                     STSEMSAKGNHGMIIHLDKVPTRQKNMEPWEILLSESQERMLIVAEKGKEHLINAVFE
                     KWDLNCTCIGEVVKGEQLKFYMNETLVADVNAESLVLGGGAPVYKRDYKEPAYFKQNK
                     TFNIDNVKVPIDLTAVAKQLASHPNICSRRWVYTQYDSMVGTINMSTNKPSDAAIVNI
                     KNSKKALAVSVDCNARYVFADPEQGCAIAVAEAARNIVCTGGEPSAVTNCLNFGNPYN
                     PEVYWQFVHSIKGMSKACEKFQTPVTGGNVSFYNQSKIGNDDVPVYPTPTIGMIGIIS
                     DKNKAATLAFSNAGDQIFLIGTSKNCIASSQYLVSTHGINNSPAPEFNLDEEYDVQQL
                     IKQLIKTGYVQSVHDVSDGGLFITLLESAMIKGLGFEINSDEDIRKDAFLFGESQSRV
                     VVSVSEEQMDEFISLVADSGVDFTNLGVVTHGEIIVDEVKFGNISEYSKLYQEKLESE
                     MAY"
     gene            20169..21464
                     /locus_tag="PKU82_02670"
     CDS             20169..21464
                     /locus_tag="PKU82_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81807.1"
                     /translation="MEAVLSIGYIALFVFVIHKSAFFRIEGIPVWWLKAAFLVKIIFA
                     ILLGVIYSKYYTDRLTADTFKFFDDSKILYDLAFEHPLWFLQAVFGINDQASYLMPYY
                     DTMHNWFNKVVLFNDYRFQIRLNALMRFISLGYYHVHAVFYCFLSFVGLTALLKLFVN
                     VLPELRKVLFAGVFFIPSVLFWGSGLLKDSLILFATGMALYFLNRLIQERKSATIFGA
                     LFFLLFLMMIKFHNFILLLPLFAAYLITSVYRKKTLLIFVSIVSVYYLILINLNLIMP
                     GFGLMELLSNKQAEFVDLANDYHANSTIAINYLAPSEWNAIVNTPQALLHSFFRPYPF
                     ESKNLFILLAGFENLFFGLMMILVLLNFKIHKQASPLFWVAVFYTLSLFELIGLVTPV
                     MGAIVRYKIQALPFLFFLLIYLTDIEKLGSRYMTKSKQT"
     gene            21466..22752
                     /locus_tag="PKU82_02675"
     CDS             21466..22752
                     /locus_tag="PKU82_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81808.1"
                     /translation="MEVFLIPLVYILLTCLLIGNHRFFELKGVSRAFVCILFIIKAFA
                     GIIITFNAEKIYSGDDGYFFYEAGKTFLDYGVENPYAYLKLMFGIYDSSNSEAYLHFL
                     NWKNLPMWNDGETMIKLCSIIHIFAFSNILVHSIVFSFLSFCGLTAIYKATIHITRSN
                     GFSLFLLLMLFPSVVFFSSGILKETLLIALSGLFFFSLTQFGVSLRMKIYCFILFFLL
                     WLIKPHFIFFFTPAIIAYITMAKLNLSSIWMSRLMILGVFALYIVLAVVVHDKVLGRN
                     AAGTIALVQQSSMKNSIYEKAESYIQPPVIAAKYSSILRNTPEAFYQVITVPWFYTNA
                     GLRWKAAAIENIFVLIMVLMNIYAAFDKKNKYALYHFAFLLFLISFYSLVGFTCALEA
                     SILRFKAPILPFLIAGMYMFLLQYKDKILKNETSDN"
     gene            22733..23608
                     /locus_tag="PKU82_02680"
     CDS             22733..23608
                     /locus_tag="PKU82_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ81809.1"
                     /translation="MKLQTIDTGFFKLDGGAMFGVVPKSIWQKLNPSDDNNMCTWALR
                     CLLIEDGNRLILIDNGIGDKQSQKFFSHYYLHGDSTLEKSLSKAGFHSDDITDVFLTH
                     LHFDHCGGSIKWNSSRDGFLPAFKNATYWCHADHWKWATEPNAREKASFLKENILPIK
                     DSGQLKFFEGTSDMSNGFEIIKKNNFHSDIDLVVARGHTDGMTLPLIPYKNGKLIFMA
                     DLLPSAAHVPLPYVMAYDTRPLVTLEEKKAFLKYAVHNKLNLFLEHDPTNECCNLLET
                     EKGVGVNSTFKLSEI"
     gene            complement(23613..25313)
                     /locus_tag="PKU82_02685"
     CDS             complement(23613..25313)
                     /locus_tag="PKU82_02685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOZ81810.1"
                     /translation="MKVAIFLTCIFTLIISNAYSQQTAIPLSPLTIPLIQKGNYGEVR
                     SNHFHSGLDFSTQTKIGFPVIASFDGYVSRIKTSENGYGKAVYIDHPNGLTTVYAHLH
                     RYGRTIEQLVFAEQEKQKKYSIELFPERNKITVRQGDTIGWSGNSGGSSGPHLHYEIR
                     NTKSEKPLNPINYFPQIKDSIPPLLKSITIYSIKEDFLSIFPWHEIKPVETSRGNYSY
                     TELISASGEIGIGFSGYDQANDDNAILGIDEAELYANEKLIYARKMSNFSFDETRNAN
                     GLIDYAKGQRTGSITERCYKLPGVKFSQYIFLKNSGVVDLENDSIINFRLMLRDGVGN
                     ISRCVFTVAYSNNAKPKNTVVKKGELLEYNKANKIQSDRYKLMLPAGALFRDFYCDAI
                     IRRGNKSFIAPQITIGNTSEGLKKNITLKIKSDLEGDSLNYKTIMVKIASNGTASPVS
                     GIYKDGWYSASISSFGTYSLMVDSISPVIENISTSFDSVYNCPVLEIKVSDNFSGIKN
                     YSCMINNKWHLSEYDRKNGTIYVLLNQFAESKFNIKIDLSDMCNNTTTLSKEIILSKK
                     "
     gene            complement(25374..26351)
                     /locus_tag="PKU82_02690"
     CDS             complement(25374..26351)
                     /locus_tag="PKU82_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOZ81811.1"
                     /translation="MNMLSKSIAVVLISILAFSQDVLAQRMKTEDYISRYKEDAIKDM
                     IKTGVPACITLAQGILESESGNSKLAIEANNHFGIKCHKEWQGKEFHQDDDAANECFR
                     KYNDVLESFDDHSDFLRSRPRYASLFELKPTDYKGWAHGLKKAGYATNPNYAHKLIDL
                     IERFNLEDINNEAIARMNGKPIPEQPTLARIVNLPKGSPSSKEVEKTTVINITPPSYY
                     SEINNVKYIVSKRGDTWLSLAKQNEMMLWQMLKYNDASKDDVLKEGTIVYIKPKRNSA
                     QQDYHVVKQGETMRDISQLYAIKLNKLYKKNQLEPGTALEPGMRIKLNN"
     gene            26536..>27058
                     /locus_tag="PKU82_02695"
     CDS             26536..>27058
                     /locus_tag="PKU82_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOZ81812.1"
                     /translation="MPQYYSQGKIPHKRHTQFRKPDGGLYAEELFSTHGFSNMYSLIY
                     HCHPPTMIKSAGEPFSREPEIAMKRSLLHQSFQGFNVQPEDDYIKSKKPVLVNNDLQI
                     ALAAPRKSTKDYFLKNADADELIFVHEGSGTFHSIYGSMDFGYGDYIVIPRGTIFQLH
                     FNTENNRLLVVDSF"
BASE COUNT         8267 a         4702 c         4973 g         9116 t
ORIGIN      
        1 taaggatgcg acatattcga aatctcaagt tttaccaatg gatattcatt tccatcttcc
       61 cacgtaatgg tgtcttttgt atcaacacat gaacgcgtta ggaacgcata atcgcaactg
      121 atatctttaa atactaccat gcggtagttc tttggatgta tttcgctttt cattttataa
      181 taaagttttt atcaaacggg ctgcaaatat agcacttttt cttattctat aacaattccg
      241 cagtaaaaat tattttaagt tactgcttta attatcagtg gattactaat gcaaggtggt
      301 taatacttct gtattgaaaa tatagacatc aaaggctaca gaccgttaaa cttgttaata
      361 tttatgaaat attgatattc aaagcattta cagtgataaa gcgctaataa aagtactatt
      421 tttgcgttca attttaaatt ttgtaaagcc tgaggaatgt ttctttttga gcgtacagca
      481 ccacgttggg tagtcttagt aatagatttg atattatgca ctttgtcatt ctatctggct
      541 tatgatttgc gttttaattt tactatccct gatgtagaaa ttcaactctt gcccacagcg
      601 tttgcaacag ttttaagtgt aagattggtt agttttttaa tatcaagaac gcataccggt
      661 ataatccgct atacaagcta caaagattcg caacgaatat ttttaacctt gctgtcggga
      721 acagttttgt tatgcatctt taaccttatt tcattcagaa tttatggacg ttattttacg
      781 ccttactcta ttctgatgat agactttttt ctgagtatgc ttgcattggc atcaatgcgt
      841 gtgattgtaa aaagtgcaca cgtggagtgg acaagaaaga agggtaataa aaccgatgtg
      901 cttattatgg gtgcaggtga agaaggaatg gttgtgaaac atgctttgga acaagatgca
      961 ggaataaatt ttaaggttct tggatttatt gatgataagc caaaactccg taaaagaaag
     1021 cttgaaggca taccggtttt ttatacaaag accgatttgg aagacttgct tcgagccaat
     1081 aaaatatcga gcctgattat tgcagataag gatgttgaca ctaagcgcat caatgaatta
     1141 attgaaattt gtgtaaagta cgatacaaaa gttcagcgtg ctccacgtgc cggaaactgg
     1201 ataaacggca aactgagttt ttctcaaatc aaggaagtaa acattgaaga cttgcttgag
     1261 cgtgatccta taaatcttga tgtaaaggaa atccgtaatc agctaaacgg aaaaactatt
     1321 ctcatcaccg gagcagcagg atctattggc tctgaaattg cgcgacaggt aattttatac
     1381 aaccccgcaa aagtggttct tctcgacatt gcagagtcgc cactttatga gcttgaactt
     1441 tccatacagg aaattaaaca tgcaggcaat tgcgaaagtg tgattgctga catccgcaac
     1501 attgaccgta tgcgcagggt gtttgattat ttcaaacctg aaattgtatt tcatgcagca
     1561 gcctataagc atgtgcctgt tatggaagtg aatccttcag aagcaattct gaccaacgtt
     1621 aaaggtacta aaattcttgc agacctgtct gttgaatttg gagtgcagaa gtttgtgatg
     1681 atttcgacag ataaggctgt taatcccaca aacgttatgg gtgcatcaaa acgtgttgca
     1741 gaaatttata ttcaatcgct cagcgagatt tccgaaacac aatttatcac aacacgattc
     1801 ggcaatgttc ttggttcagc aggatcagtg attccacgat ttaagaagca gatagaaaac
     1861 ggcatgcctg taacagtgac agatgaaaat attacccgtt tttttatgac cattcctgag
     1921 gcttgtcagc ttgtgcttga agcttgcaaa atgggaaagg gtggcgaaat ttttatattc
     1981 gatatgggta aacccgttaa gattgctgag cttgcaaaga agatgattcg cctgagtgga
     2041 ttagaacctg agaaggatat taaaattcaa tatacaggtt taagacccgg ggaaaagttg
     2101 tttgaagagt tgttgaataa acaagagaac accatagcaa cacatcatcc taaaatttta
     2161 attgccaaag tgcggaaata taaattcgat cagattgcaa gcgatattga aaaccttacc
     2221 aacctgttta agacccaaaa caatatggca ttggtttcga agatgaaaga aatagtgcct
     2281 gaatatatca gcaataacag tgagttcgaa aaactcgatc gcagagaaat ctgaaactgt
     2341 tgctgaggtt cattttcact gtgacataat cgtctgattt aaaatttttc agcaaattaa
     2401 attatcagat ttttttcaga taagattgca tcaaacattt aacaattaag gaatagtttt
     2461 taagagtaat tatgaataga tatttagata gaatagtaaa acggttttat atttttgcag
     2521 catgttagtt ttaaataata taagttttga ttttggagga agatatcttt atcatgatgc
     2581 attctggcac attaaaccca atcagaaaat aggactcata ggcgctaatg gcacaggtaa
     2641 atcaacatta ctgcggatta ttaatggaga atatacttta accgggggtg agattacagg
     2701 aagaaaagat cttactgtag gttttttaaa tcaggattta ctttcatacg aatcagacaa
     2761 aagtattctt gatgtggcac ttgaagtttt tgaaagagaa aatattctgc atcaaaagat
     2821 tgaaaaaatt ttaaaacaac ttgaaacaga acatggcgaa gagctattga acgaactgtt
     2881 ttttcttcag agtgaattcg aatctcttga tggttacaac atcacacatc gggcggaatc
     2941 aattttagaa ggtttaggtt tcagtactat tgatttgcaa cgtccgttga aagaattttc
     3001 cggaggatgg cgtatgcgcg taatgcttgc acgaatgctg ttgaagaaac caagtctgtt
     3061 aatgcttgat gagccaacca atcacctcga tttaccttcc atacaatggc ttgaaaaata
     3121 tctgcaggat tatgatggta cctttattct tgtttcgcac gacagatatt ttcttgaccg
     3181 cactatcaat atgattgcgg aagttgctaa tgaaaaaatt aatctttatc cgggtaattt
     3241 caccaactat ctcgaacaga aaactatgcg tgatgaattg cagcagcgta aattcgagaa
     3301 tcaggaaaag tatattaaag agcaacagaa attaattgac cgctttaaag caaaagccag
     3361 caaggcaaag atggctcagt ccagaatgaa aatgctcgac cgtatggaaa aagttgaagc
     3421 agtttccggc cctgcatcaa ccatgagatt aagtttacaa gtagagacac agcccggcaa
     3481 ggtagttttt gaaggacatc atctgaataa aaattatggt gatctggata tttttaaaaa
     3541 cagtcatttt acaattgaaa gaggtgataa aattgcactt ataggtgcta acggaaaagg
     3601 taagtcaacc ttattgcgca tgattgccgg cacagaaaaa gcagatggcg aaatcaagga
     3661 aggctacaat gtaaagacat ctttctatgc acagcatcag ttagaaagtt tgaatattga
     3721 gaacgatttg atgcgcgaac tgcagatttt tgctccgtta gaaaccgatc aacgtatcag
     3781 aagtattctg ggtgcatttc tttttaatcg tgacgatgtt tttaagaaaa ttcgagtact
     3841 ttcaggaggt gagaaagcac gtgtggcatt agcgaagtta attctcagca aagcaaattt
     3901 tctgttatta gatgagccta ccaaccacct cgacatgcaa agtgtagaaa ttcttactga
     3961 tgtgttgttg gattacgaag gctcttttgt agtggtttca cacgatcgtt tttttgttga
     4021 acagattgcc aataaaatat ggtttatcga gaacaatcag cttaaagaat atcccggaac
     4081 ttatgaagag tatgagcatt gggcagaacg aaaaaaatct gaaaaacgaa cagaagaaaa
     4141 acaagttgta gccaaacctg ttgcaccaaa ggtgcaggca gagcccgatc agaaaaaaca
     4201 gttgttgaat ttagagaagg aggttaaaaa attggaagca gagttggcag cagcaaagaa
     4261 gcaacgcaat gatttgatgg agcagttggg caatgaaagt aattataatg acacagctaa
     4321 aatcaaactg ttacagaagg agtttgatga atctgaaaaa cagtataata agatacattc
     4381 attgtgggaa gacagctaca tgcaatggat ggaaatgagt gaatgatgtg cttatgaaac
     4441 attttatttt ttatctgata cttttctttt tgtggtctaa ctctaatgtg tttggtcagg
     4501 aaaagcaaga atattttaaa gacaattatt tcaggtacga taactttacg tacagaagta
     4561 atattaaaac gatacaactg gaaaatgcac agttcccgat gagtgaacct gtgattgaac
     4621 tcaacagtgg agcgcagctt ttactccagt ttgacgacct tgaaggcgat tataaagatt
     4681 actggtacac catcattcat tgcgatgcac aatggaagcc ttcagacatt acgcgctcaa
     4741 catatttgtt tagcttctac gaagaccgta ttgctgatta tgctttttcg tttaacacct
     4801 tgcaacctta cactcattac tcattaaaat ttccaaacga acagattaaa ccactgatat
     4861 caggaaatta tttgctgaaa gtttatttga ataacgaccc tgacagtgtt gcatttacaa
     4921 gaagattttt agtgtatgaa aacctgattg atgttgacgc taccgtgcat gcagcaacat
     4981 tggctgaata cagacaacag aaacaggaag ttgatttttc attgtcaact acaagatata
     5041 ccatcgccaa tccctttggt gatttaaaag ttaaaattct tcagaatttc agatgggata
     5101 atgctgttga gaatctgaag ccgattttta ttaaagataa cttgctggaa tataattatg
     5161 atgaagaaaa tacttttaat ggcagtaatg agttcagacg ctttgatatt cgctcattaa
     5221 agtatgaaac agaaagtgta gagcgaatag atcattttga taccggaacc aatgtgttgc
     5281 ttttccctga taaggttcgt tatttcagtc gttatacaac cgactatgat attgacggaa
     5341 agtttgaaat aaaaaactac agtggcagcc atcctgagcg tgatgccgat tatgcaaccg
     5401 ttcgtttccg ccttgaatat aaagacgaag ttaaagatgg caacatttat atttttgggg
     5461 ctctcaccga ctggggtttt acgagtcaga acatgcttcg ttatgatgaa gacctgaaag
     5521 cttacacagc aacattgttt ttaaaacaag gctactacaa ttatatgtat gtgtttttaa
     5581 aagacggaac ttcttttgga gatgaaagct ggcttgaagg caatcactat gaaacagaaa
     5641 acaactattg gattttggta tatcatcgcc cgcaaacaga aaggtacgat aaattggtgg
     5701 cctgcagaaa atttgcttcc aacagatagt ttaaaataaa aatgggtaag ctacttatat
     5761 cgcaccgcta cgaccactta tccttgctgc attcctgccc tggggaggtt cgagggagct
     5821 ggtcgtataa gacttacccg gtgcaaagat agtaaatcat acctttcaat aaacaaataa
     5881 acacagcagg ttttatacct ttgcaatcat aaattatgtt tcgatacgga ttacattatg
     5941 gaggaatatt aggggcatca agttttgcta tgtttttgat tatttatctt acgggaaata
     6001 atccgcttgg caatgcatcg tggctcggtg catggatacc tgcagttgta attgctgttg
     6061 cagtaaaaag ttaccggaca catgagggtg agggttttat ctcttatggt gaaggtttcc
     6121 gtacaggatt gattactgct ctttttggtg gaattctttc agcattgttg atttatattt
     6181 tctgcacctt attcgacagt acattggtag agcgttttaa aactgattct cttgcacaac
     6241 ttgaacttat ggaaggacag tttaaatcaa tgctcagtga taaaatgtat gatcaggctg
     6301 ttgatgccta caataatata agtcttcagt caattgcaat gaacgatttt tataataagc
     6361 tactcagcgg aattatactt tcacttatca ttgccgcagt gtataaacgt aacaagcctc
     6421 aaattctcga ataaaatgca gtcatcatac agaatagata tttcagtagt tattccgttg
     6481 tttaatgagg atgagtctct acctgaattg gcagcatgga ttgaccgtgt tatgaagcaa
     6541 cacaagttta gctatgaagt gattatggtt gatgatggta gcaaagatca ctcatggcag
     6601 gtgattgaag acctaaataa acaaatacct gaaataaaag gtgtgaagtt taaaagaaac
     6661 tatggcaagt ctgctgcact acataaaggt tttgaaatgg cacaaggtca tgtagtaatt
     6721 acaatggatg ccgacctaca agacagtcct gacgaaattc ccggacttta taaaatgatt
     6781 gttgaagatg aatacgattt ggtgagtgga tggaaaaaga aacgtcacga tccgatttca
     6841 aaaactattc ccagcaaatt ttttaatggt gtcacccgta tggtttccgg gattgataat
     6901 ctgcacgatt ttaattgtgg gcttaaagca tatcatcata atgtgattaa gaatattgaa
     6961 atatacggtg agatgcatcg ttatattcct gtaattgcaa agtgggttgg ctttacaaaa
     7021 atcggtgaga aggttgttga acacagagcc agaaaatttg gtactacaaa atttggatta
     7081 gaaagatttg ttaatggatt tcttgatttg ctttccatca catttgtttc acgttttggt
     7141 aaacgcccca tgcatttatt cggtacttta ggttctctta tgtttattct gggacttata
     7201 ggtgcttgtt atcttggtgt ttcaaaactt tattgcgttt ataatgatat ccctgcaaca
     7261 cgattaacac agcgcccatc attttattta ttgctgacaa gtatgattct gggaacacaa
     7321 ctttttcttg caggatttct tgcagagatg attgctcgca atgataaaga ccgcaatcgt
     7381 tatattgtgg aaaaagaaat atctaacaca acaacgtaac ccaaaaattc tgttgcattg
     7441 aagctatccg ttgtcatcgt caactacaat gtaaagtact ttcttgagca atgcctcatt
     7501 tctgttggta aggctattag taatattgat gcagaagttt ttgtggttga caacaattct
     7561 gttgacggat catgtcagat ggttcgggat aaatttccat ttgtaaacgt aattgaaaat
     7621 aaaataaaca ccggtttttc aaaagcaaac aatcaggcta ttgtacaaag caaaggtgag
     7681 tatttgctac ttcttaatcc ggacactgtt gtagaggagg atacatttaa aaaggtggtt
     7741 gactttatgg acaatcatcc acaggcaggc ggacttggag tgcacatgat tgacggaaac
     7801 ggaaaatttt tgcctgaatc caaaagagga cttccgacac ctgcagtggc attctataaa
     7861 atcttcgggc tgtctgcatt gtttccaaaa tcaaaattat ttggtcagta tcatttaggt
     7921 ttcctcgaca agaataagac caatgaagtt gatgtgctca gtggtgcatt tatgttgttg
     7981 agaaaatctg tgttggataa aataggatta cttgacgaga cattttttat gtatggtgaa
     8041 gatattgact tgtcatacag aataacaaaa gccggctata aaaattatta tttccctgaa
     8101 acaaaaatca tacactacaa aggagagagt accaagaaga gcagtgtaaa ttatgtagta
     8161 gtgttttata aggcaatgag catttttgct cagaaacatt tttcgcagag caatgctgca
     8221 tttttttcat ttctgataaa tattgccatc tacctaagag caggtattgc attgatgaat
     8281 cgccttttta gtattttgtt gttaccaatt attgatgcag catttatata tggaggttat
     8341 accattatta aaacatattg ggaactttat gtcaaacaat tgcattatcc ggagcattac
     8401 atgcttttag ttgtgcccgg atatgtttta gtctggattt ttgctgctta tctgaatggt
     8461 ggttatgata aaccactgcg tatttccaga gttgtaagag gaattctgtc aggcacaatt
     8521 tttattttag ttgtgtatgc tctgttgaac gagacctacc gtttttcaag agcgctcatt
     8581 ttaatgaata cagcctgggc atgtctgata actgcgttaa tccgatatac gttaaatgca
     8641 gcaggtatta aaggatttgc tttggcagca gatgtcaaaa agaaattgat tgttgttgca
     8701 gatgctgttg atgcaaaaag aattttatca ttgctacaat tatcagggtc aaatacaact
     8761 tttgtaggat atgttgattc aggagtcact caggacggag aattgaagaa tttcagactt
     8821 ggaacattga cacaattaaa tgaaatcatt actatttatc aggttgatga agtaattttc
     8881 agttctcact cattaagctc gcagcagata atcgagaata tgggggcgct gcgtaacagg
     8941 caggttgagt ataagattgc tcctccagaa agtttgttta tcattggtag taattcaata
     9001 aatgataatg gtgatcttta tcttgttgat gtaaatacat tacacagtcc ttccaataga
     9061 agaaaaaaaa gattttttga tattttcagt gctttcggaa tattattctg ctatcctttt
     9121 ctttggctat tattcaagaa aaatcaaaat gtttttgtaa atagcattgc tgtacttttt
     9181 ggaaagaaaa catgggtagc aaccaatatt aagagtttaa cgtcaacaca tgctaaagtt
     9241 ggtgtttata cgccatcaga tggattaaac acaccatcag acaatgaata tttccgcgac
     9301 agattaaatg tgctttatac ccgtgattac aatatgatta acgactggaa tatagtacgg
     9361 agaaatattt ttaagtgaga aaatattaca aggttttaac ctgaattttt ctttcgccaa
     9421 accatttact gataaactta tttcttttac ccatgaaaaa ttcctctata aaaatagtaa
     9481 atgccattgc tgtcagaaaa gtaaaaataa gaaacaccag atgtgccatc aaacctgttc
     9541 cgattttttt gttaaaccag gttacccaaa gtggatgcca caaataccag ttgtaactgt
     9601 agtatgcaat aaatctcaac caatttaatc ctgacatatt ccagtgataa gtagctgcaa
     9661 gcatcagaaa aaaacaaaag ggtatagcag aatgaaacac gcatttcctg aaggtaatcc
     9721 attcataagc actatatgtg tacagaagta aactaaaaag aatgattcct gcaatcagca
     9781 ttgtgatttg tgtgctttgt tttactatta acttaggata aaaattgaga ataatactca
     9841 gcatcacacc ccatcctaaa gcatctatgc ggttatgtgt ggcagaatag gtatctttac
     9901 tattggtaaa agccatgaca agaaacttaa atataactcc tgctgtaatt accaataaga
     9961 ttgcagtcac caacattttt ttgtcgggga agaatctttg aattagaatc aatagcagtg
    10021 gtagcatgat ataaaaatgt tcttctacac agagcgacca caaatgccca aaactccata
    10081 cttcattacc ggtataatta acatagaaga gaatataacg taaatattta tctgccggaa
    10141 ttatcatgtc gggggcaagg gtatgatata aaacgtttgc tatgattgat cctataaaca
    10201 gatacgaaaa ataagatggc caaattttaa accctcgttg aataataaat ttgaaaaaat
    10261 ttattttctc cccttttttt aaatagccaa taagaatatt acctatcagt aatccggaga
    10321 tcacaaaaaa taaatcaacg ccaagatagc caaaaggaag ctgcctgttg taatgataaa
    10381 taacaacact tagtatcgca aatgatctga aaatatctac acttgaattt ctgccagggg
    10441 cattaattac ctcttgtgat tttagttgaa tcataaaaga atcaaggtct caaatataaa
    10501 tctatggctt caccctgatt gatggtgttg ttatcaggtg cagggttctg atcatagatt
    10561 tttgcattaa gcgtatcttt aatcacacca tcaaaaaata atgaaccaat gtttaatgat
    10621 gatcctttta gcacgaataa tgcttcgtca taagttaggc caataagaga tggaacagtg
    10681 actactgtat ttcctaatcc atcaccaaca atcaaatcaa tggcagaacc tttaggtaca
    10741 tctgttcctg cttttaaatc ttttccattt atcatcatgg agagcactgc attttttgca
    10801 agatcaggtt tgaaaatctg ctcacccatt ctaagtcctg atgcagcaag aatggcttcc
    10861 gcctgtcgta aggaaacatc tttcagtgcc ggaactttca ccattggtgc actactgcga
    10921 gttattgtaa gatagatagt acgaccttgt tttacttttt catttggttg cggatcttgc
    10981 tcaactatag ttcccggttt ttgatctaaa aggtaaaccg atgaatcagc aattttaaat
    11041 gacaagtttt tagtctttaa aaaatcatca acctttttaa tgttcatgcc ttttaaatca
    11101 ggcacagaaa ctgagttgcc atgatctgta aaactgtcaa gccatttgta aaccaaacct
    11161 attgacatga tgattacgat aataatcacc aaagcattaa tccagaaaat gcgcgactta
    11221 agaaatcgaa ttagttcttt cattatttta tttcgtcagg gataactgat ttcttttaaa
    11281 tccgaattca agtatttttt caataaactc ataaggctta tactgattta ttgcagcctg
    11341 atggaaaatg caagttgccg gtgtcatgcc gggcaatgaa ttcacttcta taaaaacaac
    11401 ttcaacttta ttgtcgattg taatacgaac aaatgcatca attcgtgcat agccttcaat
    11461 atttaacagt cgtgctgcac gcaaaagttc ttttctgatt atatcagaaa tcagtctgtt
    11521 ttccattgca tgtttgctgt aacgtgctgg agtgatattt tgtccttcac cggctaaaaa
    11581 tttttcttca agcgaaagta catcaccttc atgtaatgtt tcgctgggtt caaaaatttc
    11641 gtaatgtata tttccggcct catcatagga tgtgagcatg ccacctgtga tttctaaaaa
    11701 atgtttagtg ttttcaccac tgataagtgt ttcaataagg aaatatcttt ttcgtggaaa
    11761 ctcttctttg tcttttatct tcagtagttc agctgcagca gtgtttaatg tttcatcttc
    11821 gcgaaaaatt aattcggcat aggcatgtaa ctgattttca tttcggataa tttttactgc
    11881 actactgcat ccatcatcaa ccggtttagc aataataggg aatccaaatt ctttttgaat
    11941 tttttcttct atcgcagatg gattattacg ccattcttcc tctgtgagtt tataatgttg
    12001 tgcaacttta aatccatttt gtgccagcaa ctcatttgtt tgaaatttat caatagtgat
    12061 tttagaagat tcagcccttg agccattgaa tggtaaacca actttattaa gttgtaattg
    12121 taattcacca tcttcacccg gacgaccatg taaagcaatg aaaacaattt ttacttgttg
    12181 cgccagctct tcaaacgatg ttttttcagg aggatttata ttggccgcat ttgcaaacgt
    12241 ttgtgttacc tgaccacatt ggtcaattat ttttttaata acaggatgct gattgtaatt
    12301 gagaattttt tctctgatat cgtcagcatt atcttttagc atcaggttta ttggaatcct
    12361 gtaaagtgaa taagactgtt cgttaccggc aagaaaataa ggaatgggtt cgtacttttc
    12421 tgttgaagat aatttttcat aaacatttcg tccgctctca acactaatgt gtctttcact
    12481 gctatagcca cccattatga caccaacttt tatcttttca gattgcgatt tggctttttt
    12541 gctgagaagc ccatgcaggt tttttatcag ttgtggtacc gtacgaaatg atttcatttc
    12601 taagagacgc gcctgaagtg atgttgaaat aatgtagctg ataaactgag aagggttgag
    12661 accgatttct gcagcctgat gaaagaaaaa gctactgggc atcattcctg atgttgtatt
    12721 cggatcattc aaaaatattg taccattctc tgtaataaaa ccatctattc gggcataaac
    12781 actaaagttt aatgacgtaa acagacgttc acactctctt cttattgttt caatttgtac
    12841 ttcgggcagg tgaataggtg ttacttttct tgataaaccg ggcaggtatt tagctcggta
    12901 gtcaaacaac tcattgcctt tgcgtatttc tgttggcggt aatgcaacag gcttttcgtt
    12961 ttcatttgta attacgatgc atgaaaactc ttttcctgaa atgaatgatt caataataac
    13021 ttctgtttca gcttcaatag cagcaataga aacaacgtct tccatttcgc caaaaagttg
    13081 tatcagaact cccaataatt cttccgggtg atagattgtt ctatcattaa tttttgctgg
    13141 taaaccaata ccctcgcgaa tatcagatac tttctttatc cattctgttt ttgtatcaaa
    13201 agtcatgctg ttccattctg tccgagtgat ttcgcggata aaaaatgatt tgttgacagc
    13261 ttgtataaat tcttctacat cattattctt caatactgat attccgatac tcgaaccctg
    13321 attagcagct ttaactacac aaggaaatcc aacagatttt aaatttttaa taaaagattt
    13381 tcggtttggt gacgcaagcc attctattcg gtttatgaca gcaacttcag gaacattaaa
    13441 acctgatttt ttcatcagtt ctttctgaat aactttattc attcctattg cagaagaaag
    13501 aatacctgaa cctgagtaag ggattcggta atattccaac attccttgta tgcgtccatc
    13561 ttcgccatca gagccatgca gacataagaa tgcaaaatca attacatcat gtaactgcgc
    13621 aaactctatg cgcgttccta acttcatcgt tagtcttatt tgttcctgat gagtgagcgt
    13681 tccaagattt tctgcataaa tctgaaaacc acgtgttgtt tcgggtaaaa attctgatgg
    13741 aggataaaaa tcacgaatgc tacccttgta tataaattgc cagtcgagaa tcagtaggtt
    13801 gccaaaactg tcaacaaaaa gaggaatagg ttcaaatagt gttttgtcga ggttgtcata
    13861 tacggttctg cctccggcaa aggacacctc gcgttcgcgt gatgagccgc caaaaagaat
    13921 tcctattttt agtttgttca tataaatttt aagtcatgaa atgattgaac aaagttaaca
    13981 gattaataga gcagcataca tgctataaga gtaagtattc actatttttt taacatttat
    14041 tttgaaagca gtttttatta aatacacgtt agaaagattg ttttttaaat tggtttttgc
    14101 tcaataaaaa cgactgatat ccagagctta ttttaacaac ccattgacac ctgcaccaaa
    14161 aagtgcaaag tcgtattttg cagggtcgct tttatcaaat tgtcgaagat ttatagtcag
    14221 ttcttcaacg gctttccaat catcttgctg tcttttcagc agcccaagta gtcgtgctgt
    14281 tcgaccggta tgtacatcta aaggacaata aagtaatgag ggtgatattt cccaaattcc
    14341 aaagtcaaca ccggcacggt ctttccttat catccaacgt agaaacatat tgaatctttt
    14401 gcaacttgaa ttttttaatg ggtcgcctaa atgcttggtg gtgcgtggtt ggcagggttg
    14461 ggagaatagt ttctgcctga ataaactcat agctacaggc atgggattgt tgcttacaaa
    14521 agatgttaag caatttccta acgatccgta ttcagcataa atatttttaa tagctttaat
    14581 aatgtagata caatcttctc cattataagt gcggtgcaca aattttttaa aagggatata
    14641 atcgttgttt gatgcattta tgataaatga ttttggagta tgatccatca tttccataag
    14701 tgtttgcgca tttttcagaa tggtttttcg ctgcccccat gagataagag aggtcagaaa
    14761 ggcgctgatt tcaatatcac cactgtcaga gaacttatga gggatagaaa ttggatcatc
    14821 agcaataaaa tcacttctgt tgtatcgcag aaataaatcg tctaaataca gtttgagctc
    14881 atgatgattc atgattttct gttttcagac ttccttgcca ctgtccttac ttctaaaaga
    14941 aattgcaaca ggaacaccac tcagattgaa attttcgcga agtttatttt caagaaacct
    15001 tctgtacgaa gtttgtatgt attgcggatt gctactaaaa aagacaaaac gtggatatgg
    15061 tccgggcacc tgagtaatat acttaatatt gatgagttta cctttgacgg acggtggagg
    15121 ataattttct atgaaaggta gtattttttt gttaagctgc gatgtcggaa tatgttgcgt
    15181 tctgtttttg aaaacatcaa tagcagtttc aagtgcttta aaaatacgtt gcttgtttag
    15241 taccgaagta aagattattg gtacatcagt aaatggagca atgacagctt ttatttcttc
    15301 tgtaaatttc tttgttgaat ggttttcttt gtcaattaaa tcccatttgt tgactaagaa
    15361 cacaacaccc ttgtgattgg tttgaatgag gtgaaaaata tttgcatcct gtgctgtgaa
    15421 tcctttttct gcatctatca taaacagaca cacatcactt tgctcaatgg ctcttatgct
    15481 gcgcattaca gagtagaatt ccaaatcttc tttcactttg gctttccttc tcaatcctgc
    15541 agtgtcaact aaaataaaat caaaaccaaa atttgaataa cgaatattta tactatttct
    15601 ggtagttcct gcaataggtg taacgatact gcgttccaca ccggtaagca tattcattaa
    15661 agaagatttg ccgacattag gctgaccaat aattgtgatt cttggcaact catcttcttc
    15721 ctcatcatca ggcttaaaaa ctttaactac ggcatctagt agttcgccag tgccactacc
    15781 gtttgatgat gatatgcaat aataatcacc aagacctaat gcataaaact caactgcatc
    15841 attcagttgt ttaggggaat caactttgtt aactactaaa aatgttttct tattactatt
    15901 gcgaagtaat tgtgcaatat cctcatcaaa atcattcagg ccttcgcgcg catctgtaac
    15961 aaaaaggatg atgtctgctt cttcgattgc cagcttgact tgttttctta tttcagattc
    16021 aaaaatatct tctgagcccg atacatagcc tcctgtatca atcacagaaa aacttctccc
    16081 tgtccattca cctgtaccgt agtgtctgtc gcgtgttaca cccgattcat catgtacaat
    16141 ggcgtccata ctttctgtca acctgttgaa cagtgttgat ttccctacat tgggtctgcc
    16201 tacaatagca ataatgttgc tcatttttct ttgttattcg ttgtagccga atttgttcag
    16261 ctgttgctcg ttatttctcc agtcttctct aatctttacc gtcagttcga gaaatacttt
    16321 tttgccagtc atctcttcta ttttctttct gctgtcagtc cccaactttt ttatagccaa
    16381 gccatttttt cctaataaaa ttgctttctg cgattctctt tcaacaaaaa tataactctt
    16441 gatttttgcc agcttagggg tgtctttaaa ttcttcaata cttacttcaa cactataggg
    16501 tatttcttta tcatattgaa ggaaaatttt ctcacgaatt atttcggcag caaaaaatct
    16561 ttcattcctg tcagtcagtg tgtctttgtc aaaataagga ggtgcctcag gaataaattg
    16621 caataaggtt ttaatcaaaa cttctccacc aaatttttct ttagctgaaa taggtactat
    16681 ggcatcaggg ctaagttgtt gctcgatttt ttgaactatt ttttcaagtt cttcctgatt
    16741 acttaaatca attttattca gtgctacaac taactttttt ccggcaggca gcagtgttct
    16801 gaacttttcg gcatccatat catttccggt agcatcatac accaataaaa ttacatcggc
    16861 atctgtaaat gcactctgaa caaaggtcat cattcgttga tgtaattgat atccaggggt
    16921 gataatgccc ggtgtatcag aaaacaccaa ttgataatca ggctgtgtta tcacaccttt
    16981 tatgcggtga cgtgtggtct gagctttatg ggtaacaata ctcaaatcct gccctaaaag
    17041 tgtgttcaat agggttgatt ttcctgcatt aggttttcct actatagcta taaatcctgc
    17101 tttatgcact ttaaaaattt cctgcgaagg taaagaaaag ggggcaattt tttcagttag
    17161 ccgaattcgg tgttatttgt ttgcattgtc agcattttta atcatggctt cggcaatggc
    17221 atcagacatg gaatcattgc gtttgcagag atgtgaaaca atattttcgt aatccttttt
    17281 gtttctgttt tgcgacaatt tatcctttgc gtcaattctt gaaactgtaa tctcaaatcc
    17341 aattattccg atcagattat ctctgattac tttcgggtca atcttttcaa aaaatttagg
    17401 tttctcttct ttagattcgt aatggtcggt aagttgtatc agactttgca acagttctaa
    17461 ttccgtttga atttttaatt tcccataaca atgtatagcc tgataattcc atgtagaaac
    17521 attttcgtga ctataccatg aggatgaaat gtagcaatgc ggaccactaa aaattgttag
    17581 tgtttcacca gcattaatta aagtactgca ttgtggattt tctttagata tatgcccatg
    17641 taatacaaga ttgctgtttg ttactaaagg tgtcattgga atgtgggtag caaacatttc
    17701 agttgctgaa tgagttatta aaattccaaa actgttttct ttaataaagt caacttgttg
    17761 gttaaaattc gtatttcgaa aatattcagg aatgtacata attgaaaaga atacttttag
    17821 taagccatct cactttcaag tttttcctga tagagttttg aatattcgct gatgttgcca
    17881 aatttaactt cgtcaacaat tatttcacca tgtgtcacaa cacccaggtt ggtgaagtcc
    17941 acgcctgaat cagctactaa gctgataaat tcgtccattt gttcttcaga aacactaact
    18001 acaacacgac tctgtgattc tccaaaaaga aatgcatctt ttcttatatc ctcatcagag
    18061 ttaatttcaa aacccagacc ctttatcatt gcagattcaa gtaaagtaat aaataatcca
    18121 ccatcagaaa catcatgtac agattgtaca tatcctgttt tgattaactg cttgatgagt
    18181 tgttgaacat catattcttc atcgagatta aattcaggcg cagggctgtt gttgatacca
    18241 tgtgtgctga caagatattg tgatgaagca atacagtttt tagaagtgcc aatcagaaat
    18301 atctgatcac ctgcatttga aaatgccaat gttgcagctt tgtttttatc actgataatt
    18361 ccaatcatgc caatagttgg agtagggtaa acggggacat cgtcatttcc gattttcgac
    18421 tgattataaa aactaacatt accacctgtc accggagttt gaaatttttc gcatgctttt
    18481 gacatacctt ttatgctatg cacaaactgc cagtaaactt caggattata tgggttgcca
    18541 aagttaaggc aattggtaac agcagaaggc tctcctcctg tacagacaat attgcgtgct
    18601 gcttctgcaa ctgcaatagc gcagccttgt tcaggatctg caaaaacata tcttgcatta
    18661 caatcaacag aaactgcaag tgctttttta gaatttttaa tatttacaat tgcagcatca
    18721 gatggtttgt ttgtactcat attaattgtg cccaccatac tgtcgtattg tgtgtaaacc
    18781 catcgtctgc tacaaatatt cggatgtgat gctaattgtt tggcaacggc agtaagatca
    18841 ataggaactt ttacattatc aatgttaaat gttttgtttt gtttgaagta tgcaggctct
    18901 ttataatctc ttttatagac aggagcaccg ccacctaata caagtgattc agcattaaca
    18961 tctgcaacca acgtctcatt catatagaat tttaattgct cgcctttaac tacttcgcca
    19021 atgcatgtgc agtttaaatc ccatttttca aaaaccgcat taattaaatg ttctttacct
    19081 ttttctgcaa caatgagcat tctttcctgc gactcggaaa gtaaaatttc ccaaggttcc
    19141 atgttttttt ggcgcgtagg aactttgtca aggtgtataa tcatgccatg atttcctttg
    19201 gcagacattt ctgaggtaga acaaataatt cctgcagcac ccatatcctg cattcctaca
    19261 actgcaccgg ttgcaatgac ttcaagtgtt gcctctaata aaagtttttc ctgaaatgga
    19321 tcgcctacct gcacagatgg taaatcctca tgcgaatctt catgcaggtc gcccgatgca
    19381 aaagtggcac catgaatacc gtcttttcct gttgctgagc ctacaatata aacactgttg
    19441 ccttcgcctt ctgatattgc ttttacagtt ttgccatgct caacaattcc tacactcatg
    19501 gcattgacta aaatgtttgt attgtagcaa tcttcaaaat aaacttcgcc accaactgtt
    19561 ggaacaccaa aggcattacc atagtcacca attcctttaa caacaccgcg cataagcatt
    19621 tttgtgcgct cattattcag attgccaaaa cgcaaagagt taagttgtgc aataggtctt
    19681 gcacccatag taaaaatatc acggttaatt ccacccactc ctgttgcagc tccctgatag
    19741 ggttctatgg cagaagggtg gttgtgcgat tcaattttaa atgcacaggc aagattgtcg
    19801 ccaatatcaa caagacctgc attttcttct cctgctttgg ctaacatgtg aggtccttcg
    19861 cgtggcaaag ttttcagcca ggtaatagaa tttttataag agcaatgttc actccacatc
    19921 acagagtaaa tactcaattc attaaaatta ggcttacgac ccattagtga gcagatttta
    19981 tcaaactctt ccggtaaaag acctaatttt tttgctgttt caaccgttgt ttctttcaaa
    20041 ctttccattc tttattttta aagtataaat tcgtttcaga atacttttat gttactttgc
    20101 gcaaaggtaa cctattgatg taataaacag gttattgtat tgtatgaaaa ttttcaaaca
    20161 accttacttt ggaagcggtt cttagcattg gatacatagc gttatttgtt tttgtcattc
    20221 ataaatcggc atttttcagg atcgaaggaa ttccggtatg gtggcttaaa gcagcgtttc
    20281 ttgttaaaat aatttttgcc attttattag gggtaatcta ttcaaaatat tatacagaca
    20341 gactgacggc agataccttt aagttttttg atgatagcaa gatattatat gaccttgctt
    20401 ttgagcaccc actttggttt ctgcaggcag tttttggtat taatgatcag gcaagttacc
    20461 tgatgccata ttatgatacc atgcacaatt ggttcaataa agtagttctg tttaatgact
    20521 atcgttttca gataagattg aatgcgttga tgcgtttcat cagtttaggt tactatcatg
    20581 tgcatgctgt gttttattgt ttcttgtcat ttgttggact cacagcatta ctcaaacttt
    20641 tcgtaaatgt acttcctgaa ctcaggaaag tactgtttgc cggagtattt tttattcctt
    20701 cagttttatt ttggggttct ggattgttga aagattcact gatattgttt gcaacaggaa
    20761 tggctcttta ctttttgaat cggttgattc aggagagaaa atctgcaaca atttttggtg
    20821 ctttgttttt tttacttttt cttatgatga ttaaatttca caattttata ttgctgttgc
    20881 cactctttgc agcatatctt attacttcag tttacagaaa aaagactttg ttgatttttg
    20941 tttcgattgt ttcagtttat tatttgatat taattaatct taatttaatt atgccgggct
    21001 ttggtttgat ggaattgttg tcaaacaagc aggccgaatt tgttgacttg gcaaatgatt
    21061 atcatgcaaa cagtactata gctataaatt atttagcacc atcagagtgg aatgcaattg
    21121 taaatacacc gcaagcattg ctacactctt ttttcagacc ctatccattt gagagtaaaa
    21181 acttattcat tctgttggca ggatttgaaa atttattttt tggactcatg atgattttgg
    21241 tattattgaa ttttaaaata cataagcagg catctccatt gttttgggtt gcagtgtttt
    21301 atacactttc tttgtttgag ctaataggct tagtaacacc tgttatgggt gcaattgtca
    21361 gatataaaat tcaagccttg ccatttctat tctttctatt gatatatttg acagatattg
    21421 aaaaattagg aagcagatac atgactaaat ctaagcaaac ataagatgga agtatttctt
    21481 atccctttag tttacatctt attaacttgc cttttaattg gtaatcacag attctttgaa
    21541 cttaaagggg tatcgcgtgc ttttgtttgc attcttttta tcattaaagc atttgcaggt
    21601 attatcatca cttttaatgc agaaaaaatt tacagtggtg atgatgggta ttttttttac
    21661 gaagcaggta aaacatttct tgactatgga gttgaaaatc cttatgccta tttgaagctg
    21721 atgtttggga tttatgatag ttcaaatagc gaggcctatc ttcatttcct gaattggaaa
    21781 aatctgccaa tgtggaatga tggagaaacc atgattaagt tgtgttccat catacacatt
    21841 tttgcttttt caaatattct ggtccactct attgttttta gctttttatc tttttgtgga
    21901 ctgacagcaa tttataaagc caccattcat ataacacgat caaacggctt tagtcttttt
    21961 ctgctgctta tgctttttcc ttctgtagtt ttttttagtt caggaatttt aaaagaaaca
    22021 ctattgattg cattatcagg tttgttcttt ttcagtctta cacagtttgg agtgtctttg
    22081 cgaatgaaaa tttattgttt tattctgttc tttttacttt ggctgataaa acctcatttt
    22141 attttctttt ttaccccggc aattatcgca tatatcacta tggcaaagtt gaatttgagt
    22201 tcaatatgga tgtcgcgatt gatgatactt ggtgttttcg cattgtacat tgtacttgct
    22261 gtggtagtgc atgataaagt tttaggacga aatgccgctg gtacaatagc cttggtacaa
    22321 caaagttcaa tgaagaattc tatttatgaa aaagcagaaa gctacattca acctccggtt
    22381 attgcggcaa agtacagtag tattcttagg aatacacccg aagcttttta tcaggttatc
    22441 acagtgccct ggttttatac taatgcaggt ttacggtgga aagctgctgc aatagaaaat
    22501 atctttgtgc ttattatggt gttgatgaat atttatgctg catttgataa aaagaataaa
    22561 tatgctcttt atcattttgc ttttctgttg tttcttattt ccttttatag tcttgttgga
    22621 tttacttgtg cacttgaagc ttctatttta cggtttaaag ctccaatact cccttttctt
    22681 attgcaggta tgtatatgtt tttacttcag tataaagata aaattttaaa gaatgaaact
    22741 tcagacaatt gatacaggtt tttttaaatt agatggtggt gctatgtttg gtgtagtgcc
    22801 gaaatctatt tggcaaaaat taaatccatc agacgataat aatatgtgca catgggcatt
    22861 gcgatgtctg ttgattgaag atgggaatag actgatatta attgacaatg gtattggcga
    22921 taagcaaagc caaaagtttt tttctcacta ttatcttcat ggtgacagca cattagaaaa
    22981 atccttgtca aaagcaggtt ttcattctga tgatatcact gatgtatttt tgacacatct
    23041 tcatttcgac cattgcggag gaagtattaa atggaattca tcaagagatg gatttttacc
    23101 tgcatttaaa aacgcaactt attggtgtca tgcagaccat tggaagtggg caacggaacc
    23161 aaatgcaaga gagaaggcaa gctttttaaa agaaaatatt ctgccaataa aagatagcgg
    23221 gcaattaaag ttttttgaag gtacatcaga tatgtcaaac ggatttgaaa taattaaaaa
    23281 gaacaatttc cattcagaca tagaccttgt tgttgctagg ggtcatactg atggcatgac
    23341 cttgcctttg attccatata agaatggaaa attgatattt atggctgatt tattaccttc
    23401 tgctgcgcat gttccattgc cttacgttat ggcatacgac acaaggccac ttgttacatt
    23461 agaagagaaa aaagcatttt tgaaatatgc tgtgcacaat aagcttaatt tgtttttaga
    23521 gcatgaccca actaatgagt gttgtaatct tttggaaaca gaaaaaggtg tgggtgtaaa
    23581 ttccactttt aagctaagcg agatttaaga ttttatttct tagataaaat aatttccttt
    23641 gacaatgttg ttgtgttatt gcacatatca ctaaggtcaa ttttaatatt aaatttactc
    23701 tctgcaaatt ggtttaataa aacatagata gtgccattct ttctgtcata ttcagataag
    23761 tgccatttat tgttaatcat acaggaataa ttcttaattc ccgaaaaatt gtctgatact
    23821 ttaatttcaa gaaccggaca attataaact gagtcgaatg aagttgaaat attttctatg
    23881 acaggactaa tgctgtcaac cataagtgag taagtgccaa aactgctgat gcttgctgaa
    23941 taccaaccat ctttataaat tccggatacg ggtgatgctg ttccgtttga agcaattttt
    24001 accattatgg ttttgtaatt gagcgaatcg ccttcaagat cagattttat ttttagagtt
    24061 atattctttt tcaatccttc tgatgtgttt ccaattgtta tttgcggagc aataaatgat
    24121 ttgtttcctc tgcgaattat ggcatcacaa tagaagtcac gaaataatgc tcctgcaggt
    24181 aacattagtt tataacggtc agattgaatt ttgtttgcct tattatattc caacaattct
    24241 ccttttttta caactgtatt tttaggcttg gcattgttgc tataagctac tgtaaagaca
    24301 cagcgtgaaa tatttcctac accgtcacgc aacattagtc tgaaatttat tatactgtca
    24361 ttctctaaat caacaacacc actattttta agaaaaatat actgactgaa ctttactcca
    24421 ggaagtttgt aacagcgttc ggtaatgctt cctgttcgct gaccttttgc atagtcaatg
    24481 agtccgtttg catttcgtgt ttcatcaaaa gaaaagttac tcatttttcg agcataaatt
    24541 aatttttcat tggcatatag ttctgcttca tcaattccca aaattgcatt gtcatcgtta
    24601 gcctgatcat agcctgaaaa tcctattcca atttcaccgg atgccgatat taattcagta
    24661 tagctataat tccctcgaga cgtttcaaca ggtttaattt catgccatgg aaaaatgctc
    24721 aaaaaatctt ccttaataga atagattgta attgatttaa gcagcggtgg aatgctgtct
    24781 ttaatctgag gaaagtagtt gatagggttt aaaggttttt cgctttttgt attacgtatt
    24841 tcataatgaa gatgcggacc ggatgaacca cctgagtttc ctgaccagcc aattgtgtcg
    24901 ccttgtctga cggtgatttt atttctttca ggaaacaatt cgatactgta ttttttttgt
    24961 ttctcctgct cggcaaagac taattgttca atagttctcc cataacgatg cagatgtgca
    25021 tatacagttg taagcccgtt gggatgatca atgtaaactg ctttaccata tccgttctca
    25081 gaagttttta ttcgtgagac atatccatca aaacttgcta ttacgggaaa tccgattttt
    25141 gtttgtgtag aaaaatcaag accggaatga aaatgattac ttcttacttc gccatagttt
    25201 cctttctgaa ttaatggaat tgtaagcggg cttaatggta ttgccgtctg ctgtgaataa
    25261 gcattactga ttattaaagt aaaaatacag gttaaaaaaa ttgcaacttt catatttaaa
    25321 aaacatgccg gaccaaacat tcgtttagcc cggcagtctg gtggttgttg tttttagtta
    25381 ttgagtttta ttctcatacc cggttcaagt gcagttcccg gttctaattg atttttcttg
    25441 taaagtttat ttaacttaat agcatatagt tgtgatatat cacgcatggt ctctccttgt
    25501 tttacaacat gataatcctg ttgtgcagag tttctttttg gtttgatgta aacaatggtg
    25561 ccttctttaa gtacatcatc ttttgatgca tcattatatt ttagcatctg ccaaagcatc
    25621 atttcatttt gcttggctaa actcaaccat gtatcgcctc tttttgaaac tatgtatttt
    25681 acattattga tttctgagta atatgatgga ggtgtgatat ttataacagt agttttctca
    25741 acttcttttg aagatggcga tcctttaggc agatttacaa tacgtgccag cgttggctgt
    25801 tccggaattg gtttgccatt cattcttgca atggcttcgt tgttgatgtc ttcaagattg
    25861 aaacgctcta ttaaatcaat gagtttatgt gcataattcg gattagtagc atatcctgct
    25921 tttttcaaac catgtgccca tcctttgtaa tctgtaggtt taagttcaaa caaagatgca
    25981 taacgtggtc ttgaccttaa aaaatccgaa tggtcatcaa acgattccaa tacatcattg
    26041 tattttctga aacattcatt cgctgcatca tcatcctgat gaaattcttt gccttgccat
    26101 tctttatgac atttaatacc gaaatgattg ttggcttcaa tggctaattt gctgttgccg
    26161 ctttcactct ccaatatgcc ttgtgctaaa gttatacaag caggaactcc tgtctttatc
    26221 atatccttaa tagcatcttc tttatagcga ctgatatagt cctcagtctt catgcgttgc
    26281 gccagcacgt cttgcgaaaa tgcaagaatg ctgattaaaa caactgcaat gcttttgctt
    26341 aacatattca tattcaataa ccgggtaaat tgtattttat tttacaaggt taagctaaaa
    26401 atcgcctgtt tatcggtagt gttaatggtt agtgttaaca tgcaactgtt gaaaatcaga
    26461 ctgaaacata ttgttttaag acagcaggtt tcttgcgcga attactttaa ctttgcatga
    26521 ttacattctt acatcatgcc ccaatactat tcacagggaa aaatccctca caaaagacat
    26581 actcagttcc gcaagcctga tggtggactt tatgcagaag agttgttttc tacacatggc
    26641 ttcagcaata tgtattcact catctatcat tgccatccgc caacaatgat taaaagtgcc
    26701 ggagagcctt tttcgcgcga gcccgaaatt gcaatgaagc gttcgttgtt acatcaaagc
    26761 tttcagggct ttaatgtaca gcctgaggat gattacataa aaagtaagaa acctgttttg
    26821 gtaaataacg atttgcagat agcattagct gctccccgta agtcaactaa agattatttt
    26881 ttaaaaaatg ccgatgctga tgaattgatt tttgtgcatg aaggtagcgg aacatttcac
    26941 tctatttatg gaagcatgga ctttggatat ggcgattata ttgttattcc gagaggaact
    27001 atttttcaac tgcattttaa tacagagaac aacagattac tggtagtgga ttctttta
//
LOCUS       DAOOOL010000017        26208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_140_length_26208_cov_113.759875, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000017 DAOOOL010000000
VERSION     DAOOOL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26208
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_26208_cov_113.759875"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKU82_02700"
     CDS             <1..458
                     /locus_tag="PKU82_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ81813.1"
                     /translation="KNTDIGAINSREQLNKIKALVKVGVNEGGDCYQSSCTLPSKGFW
                     FKPTIFTGVAQSSRIVQEEIFGPVLSIQTFRTVEEAVEKANNTPYGLSGGVWSDKGSK
                     VFKVTSKIKAGVLWANTYNKFDPTSPFGGFKESGMGREGGMHGLSAYLK"
     gene            477..1397
                     /locus_tag="PKU82_02705"
     CDS             477..1397
                     /locus_tag="PKU82_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ81814.1"
                     /translation="METKQKEKIKKVAVGLNGHSHHAETEIRLAVQKTYKLYIDGKFP
                     RTESGRYYKLNDKNGKVIANICRASRKDFRDAVVVARKAQGDWQKKSAFNRSQILYRI
                     AETLETRRLQFVSTLGLQGVSETAAQKEVSAAVDMLVYYAGWCDKYQQVFSSVNPVES
                     SHFNFSFPEPTGVVTAIASPEQGLLNLVSLMAPSLAGGNSIIILAAEKFPLTAIDFAE
                     VLNASDVPGGVVNILTGYSKELHTHFSSHMDVNAIVYAGDNSAELKTIQTNAAGNVKR
                     VVVVQNIAEESPYRIMELQEVKTTWHPVGI"
     gene            1562..2785
                     /locus_tag="PKU82_02710"
     CDS             1562..2785
                     /locus_tag="PKU82_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81815.1"
                     /translation="MKTFFNLIFFLFVGMNLKAQTYGCTDSLANNYNANATANDGSCI
                     YNSANVVPLSSNLLPIALDETSGLTFWNNMLWSLNDDADNNLYAIDTGNFNNYTAYPL
                     NEPNIDWEEISQDDDYFYIGDFGNNVSGNRTDLKILKIEKNSLLQNNPNVDTINFSYS
                     TQTNFNPTAINSTDFDCEAFVITADSIYLFTKEWISNHCSVYSLPNKSGSYSANFISS
                     YNVNGLVTGATQIQNSRAVVLSGYSTVMQPFVLLLYDYQGFNFFSGNKRIINVNLPFH
                     QTEGIATVNGLKYYLSNEKLVQTPINIQAKIHEIDLTPYLSHYLNSVSGSFDISENPY
                     AINIYPNPTTDFINVKWNTGVKVARIKIFNISGKEVMNQIVTEKYFQIDIRSFEKGIY
                     FLSVNDSVFRKVVKM"
     gene            2917..4293
                     /locus_tag="PKU82_02715"
     CDS             2917..4293
                     /locus_tag="PKU82_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ81816.1"
                     /translation="MKVISADDKSATSLSDSAQLRYITFILLYFSQGIPEGLTLFAIP
                     AWLAFNGKSAVEIAAYSATIMIPFSLKILLAPIIEKYTYLPMGRRRPWLLFGQFGIMS
                     GLIALAFVPEPLNNIFVIICVVLCMHVFVMFQDIATDSLVIDIVPVDQQGRANSLMWG
                     SKTIGSSVSLFLGSWLINEYGFASAFVILSVPILVVMFVPLLLRERKGEKLLPWTHGQ
                     TSDEAKQFVVESWKKMFKSFKQVVLLRNSILLLSSVFIALAAIYFINTLLPIFTIQKL
                     GWTNVYFSKIFSSCRLIGGVSGMLVGALVIQRMGVIRFIQSSLILMIVIAILFVMGAP
                     FWHHLNMIIGFITTFCILATLINIGVLALAMQLCWKRVSAIQFTFCMTIFNAGFATGA
                     ALLGWLKPYMDWQQLFLVFACLIAFTMLLFKFMKTSNHVKQVEKLEDQYIDVLKAEGS
                     LLVKSETV"
     gene            complement(4327..5679)
                     /locus_tag="PKU82_02720"
     CDS             complement(4327..5679)
                     /locus_tag="PKU82_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81817.1"
                     /translation="MSNNHILKSLLLAVIMIAGCKPELKELKFDNNSIDFSKYVAIGS
                     SYTAGYQNNALFDMGQQNSFAAILASQLKKVGGGEFLQPLVSGNDGAGFNFFLQTYIP
                     QTKLGYGTTCSSAPGFSTFPSNVTDIVAVETYIGNNAPYNNMGVPGMKSSDLDNANFG
                     STDLLLSKNPFYARFATNPETSTVLTDAVRQLPTFFSFWVGFNDIYDCAMAGSNQTLA
                     TITPLANFQNNINNALTSLIAGNRKGVVANIPDLLDLPYFTSIAYDALVLTEQQAQDL
                     NALYASNNPPITFQAGNNPLVIADPSAASGKRQMKPGELVLLPVTLDSINCAGWGSNT
                     QKPLGNRYVLTTSEINTLRNTITAYNNYLKTKADENNLAYVDMYAFFKKINNGFPHNG
                     VTYSSEFLKGQFFSLDGITPTGRGSALIANEFIKAINEKYQTRIPQTDANSFPGILFP
                     "
     gene            complement(5707..6930)
                     /locus_tag="PKU82_02725"
     CDS             complement(5707..6930)
                     /locus_tag="PKU82_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOZ81818.1"
                     /translation="MKKFISGFSILFLFCSLHVSGGGFQMQPQSHSSAGMAGAVIALQ
                     TDGSVIFYNPGGMVFLEKSNVFGGISYSQSRMAFRSNNSGLVNRTESKNMLPFYVGGT
                     IMLSEKMAAGIVVNTPFGYNTTWKSDWEGRNIILSAKLNTIFIQPTFSYKISEKLGIG
                     AGFVFASANYKWNRALIVADGADAQLDGKATGFGFNAGINYAINKKLTAALAYRSKVK
                     LNFKNTDVTFSNIPASLTNQYPASANAAAEMPLPSVLSIGFSYEVIKGLKLSFESNLN
                     GWSSFDSLNYEFDAPNTALNQEFRQARKYRDALTLRLGANYKISDKVTGRAGIAFDQT
                     PVKENYMTPDMPDGDRYVFGIGASFNVSKTFVIEGSYNFETKAEREGVNKDTGLNGAF
                     NALSHCFGVGVNYSF"
     gene            7133..9058
                     /locus_tag="PKU82_02730"
     CDS             7133..9058
                     /locus_tag="PKU82_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOZ81819.1"
                     /translation="MLVKKLFLILFSVATTLTCAASVGDTTFVSSHTNDTVVTNPNTG
                     SNGYPNWVVFPSASTNYRKIILTLTHRCPSPMSCGEWDYLDYIYIRRTGGQNNPSQDI
                     ELARYITPYGNTFNSTFKSSWTLDVTDFASFLHDSVEIEYIHTGYETNVGRGWLVSVD
                     FALIEGTPVMNPIAFKRLWHGSFPYGNASNPIENYLGADTVTLNFNTANLRLNVTQSG
                     HGADANYCAEFCSKTRTIYFDNSLVDTKQVWRLCGTNPIYPQGGTWVYDRANWCPGNW
                     VYPAVNNFAVAGGSTHIIDMNMQNYNVTSPSANYVIEGQLFEFGPLNNNIDAAIDEIL
                     APTDKFEFSRSNPTCNNPKIRIKNNGSTAITSATIKYGLVGEPESTFNWTGSINTFAT
                     ADVDLPGLISYTSGNRIFKAYIADVNNTADQYVYDDTAYSNAYVPEVMDTVFILLVKT
                     NNYPTENGYKLYDENNNIIVQRLPGSMAANTIYRDTIHATPGCYRYEITDSGDDGLYW
                     WANSAQGTGYHRILRLNNSIARNFERDFGSRYSYRFVSAPGAVVGINNQERYADIVVY
                     PNPTKGIINADITLTSASPLEVSVYNPLGQRVAVQNRTNFNAGIITFDLENQPKGVYL
                     VKVVANDQVKVQRVVLE"
     gene            9149..10492
                     /locus_tag="PKU82_02735"
     CDS             9149..10492
                     /locus_tag="PKU82_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOZ81820.1"
                     /translation="MKDMKRCNQFSLTLFLMLFALFANAQKHKTLDQFTDIDLRGSLN
                     AVYKLDSTWSIEVPEDNVTTQWSLENKTLVLSGAGSNIIVRSPDLQKINISGSGSISA
                     EDTMELHDLNIDISGSGKFKMNYVKAKHIEANMRGSGKIILGGSAQSLNANLAGSGKI
                     ITHKLNVDRAEVNVSGSGTSSVWVSDSLSANIAGSGNLYYVSSPRTINQAIAGSGKIE
                     PITSAIESELNSNSITISGSNKSGTYWSGFELGFNGLYNSKSKFDTPDGYDFLEMIPE
                     KSLAVNINFYEYDVKLIKRYVIASTGIGLSYNNYRFRKNMSLIPDTNALLYTQDTMNL
                     RKNKLTVSYATVPVLLTFNTSSNLKKAFHITTGLLFSYKLGSHTKKVYNKDGKKEKDK
                     TFDDFNIDPFRYDATVRIGYSDFTVFANYALSELFKSGKGPELHPWTIGVSLVPW"
     gene            complement(10564..11598)
                     /locus_tag="PKU82_02740"
                     /pseudo
     CDS             complement(10564..11598)
                     /locus_tag="PKU82_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
     gene            complement(11709..12932)
                     /locus_tag="PKU82_02745"
     CDS             complement(11709..12932)
                     /locus_tag="PKU82_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOZ81821.1"
                     /translation="MKKFISGFSILFLFCSLHVSGGGFQMQPQSHSSAGMAGAVIALQ
                     TDGSVIFYNPGGMVFLEKSNVFGGISYSQSRMAFRSNNSGLVNRTESKNMLPFYVGGT
                     IMLSEKMAAGIVVNTPFGYNTTWKSDWEGRNIILSAKLNTIFIQPTFSYKISEKLGIG
                     AGFVFASANYKWNRALIVADGADAQLDGKATGFGFNAGINYAINKKLTAALAYRSKVK
                     LNFKNTDVTFSNIPASLTNQYPASANAAAEMPLPSVLSIGFSYEVIKGLKLSFESNLN
                     GWSSFDSLNYEFDAPNTALNQEFRQARKYRDALTLRLGANYKISDKVTGRAGIAFDQT
                     PVKENYMTPDMPDGDRYVFGIGASFNVSKTFVIEGSYNFETKAEREGVNKDTGLNGAF
                     NALSHCFGVGVNYSF"
     gene            13135..15060
                     /locus_tag="PKU82_02750"
     CDS             13135..15060
                     /locus_tag="PKU82_02750"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOZ81822.1"
                     /translation="MLVKKLFLILFSVATTLTCAASVGDTTFVSSHTNDTVVTNPNTG
                     SNGYPNWVVFPSASTNYRKIILTLTHRCPSPMSCGEWDYLDYIYIRRTGGQNNPSQDI
                     ELARYITPYGNTFNSTFKSSWTLDVTDFASFLHDSVEIEYIHTGYETNVGRGWLVSVD
                     FALIEGTPVMNPIAFKRLWHGSFPYGNASNPIENYLGADTVTLNFNTANLRLNVTQSG
                     HGADANYCAEFCSKTRTIYFDNSLVDTKQVWRLCGTNPIYPQGGTWVYDRANWCPGNW
                     VYPAVNNFAVAGGSTHIIDMNMQNYNVTSPSANYVIEGQLFEFGPLNNNIDAAIDEIL
                     APTDKFEFSRSNPTCNNPKIRIKNNGSTAITSATIKYGLVGEPESTFNWTGSINTFAT
                     ADVDLPGLISYTSGNRIFKAYIADVNNTADQYVYDDTAYSNAYVPEVMDTVFILLVKT
                     NNYPTENGYKLYDENNNIIVQRLPGSMAANTIYRDTIHATPGCYRYEITDSGDDGLYW
                     WANSAQGTGYHRILRLNNSIARNFERDFGSRYSYRFVSAPGAVVGINNQERYADIVVY
                     PNPTKGIINADITLTSASPLEVSVYNPLGQRVAVQNRTNFNAGIITFDLENQPKGVYL
                     VKVVANDQVKVQRVVLE"
     gene            15151..16494
                     /locus_tag="PKU82_02755"
     CDS             15151..16494
                     /locus_tag="PKU82_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOZ81823.1"
                     /translation="MKDMKRCNQFSLTLFLMLFALFANAQKHKTLDQFTDIDLRGSLN
                     AVYKLDSTWSIEVPEDNVTTQWSLENKTLVLSGAGSNIIVRSPDLQKINISGSGSISA
                     EDTMELHDLNIDISGSGKFKMNYVKAKHIEANMRGSGKIILGGSAQSLNANLAGSGKI
                     ITHKLNVDRAEVNVSGSGTSSVWVSDSLSANIAGSGNLYYVSSPRTINQAIAGSGKIE
                     PITSAIESELNSNSITISGSNKSGTYWSGFELGFNGLYNSKSKFDTPDGYDFLEMIPE
                     KSLAVNINFYEYDVKLIKRYVIASTGIGLSYNNYRFRKNMSLIPDTNALLYTQDTMNL
                     RKNKLTVSYATVPVLLTFNTSSNLKKAFHITTGLLFSYKLGSHTKKVYNKDGKKEKDK
                     TFDDFNIDPFRYDATVRIGYSDFTVFANYALSELFKSGKGPELHPWTIGVSLVPW"
     gene            complement(16566..17726)
                     /locus_tag="PKU82_02760"
     CDS             complement(16566..17726)
                     /locus_tag="PKU82_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ81824.1"
                     /translation="MSRILIIDDEKSIRHSLKEILEYENYKVDEAPDGIEGIKMAEKE
                     KYDIIFCDIKMPRMDGIEVLENLQNKCPEVPVVMISGHGNIDTAVEAIRKGAFDFIAK
                     PLDLNRILITVRNALDRTVLVKETKTLKRKISRTNEMIGESEGIRKIKDMIDRVAPTD
                     ARVMITGSNGTGKELVARWIHEKSSRNNMPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKTRSGKFEQANGGTIFLDEIGDMSLSAQAKVLRALQENRITRVGGEKDFPVDVRII
                     AATNKDLKSEIEKNNFREDLYHRLSVILIHVPALDDRREDIPVLTNYFLKEIADEYKS
                     PAKEIAPDAIEQLQKKNWTGNIRELRNVVERLVILGGKTITKDDVINYVGVK"
     gene            complement(17757..18677)
                     /locus_tag="PKU82_02765"
     CDS             complement(17757..18677)
                     /locus_tag="PKU82_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOZ81825.1"
                     /translation="MNIAVIDCGTNTFHLIIAGISEGSFKILYRKNLAVKLGEGGISN
                     NKIARAAFGRGIDALKEFAADMKQFIIDKQLCYATEAIRRAANGKDFVEEAKKQSGIT
                     LEIIDGLREAELIYSGVSHAVPFGKEKVLIMDIGGGSTEFVIAGNGKMLWRKSFPLGA
                     ALLLDMFKPSDPIHEVQEKIITEHINSVLFPLTEALKQYGPVNTLVGSAGSFDTFLDL
                     CLHCFHKIKANTTWWPLDSEEYFDVHQILLTSTLQQRLEMPGMIAMRADMIVVASMLL
                     HYVIHKHNMNKLLVSAYALKEGMLYFAMNK"
     gene            complement(18683..19999)
                     /locus_tag="PKU82_02770"
     CDS             complement(18683..19999)
                     /locus_tag="PKU82_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ81826.1"
                     /translation="MNIILLIIQREYLTRVKKKSFIIMTILGPLLMGGLIAGFAYLSM
                     NQGDSISMIRVVDETGVLAGKLNNSKTVIFTKDTMPIQTARHLFNSNTDYGILYLPAN
                     ALQKPDATVLYTEKQPNISVVEYLESELSKKIENMKLTDAGIQKSTLDSIKTDISLQQ
                     KPVDPSGKDKSFSAGVSAGIGFGAGLLIYMFIFMYGIQVMRGVIEEKTSRIVEVIISS
                     VKPFQLMMGKIIGIALVGLTQFLLWVVLTMAISGTATSLIVGKDQSKLMEMQQKQMVP
                     NANIPQEKMQPASMDFMKIFEGFNITKILLCFVFYFLGGYLLYSALFAAIGAAVDAET
                     DTQQFMLPITIPLIFAYVVSTIVMTNPESELGYWFSIIPFTSPIVMMVRMPFDPPIID
                     IVISMVLLVAGFIFTTWIAAKIYRTGILMYGKKPTYKELFRWLFYK"
     gene            complement(19996..20925)
                     /locus_tag="PKU82_02775"
     CDS             complement(19996..20925)
                     /locus_tag="PKU82_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ81827.1"
                     /translation="MNLLSIREVSKIYANHKALDSVSIEIPESSVFGLLGPNGAGKTS
                     LIRIINQITGPDGGSILFGGQTLNPSHISEIGYLPEERGLYKKMEVGEQAIYLARLKG
                     LSRADALKKLRYWFEKFELQSWWRKKVEELSKGMQQKVQFIVTILHEPKLLILDEPFT
                     GFDPINANLIKDELLGLRQKGATIILSTHRMESVEELCTHIALINKSKKILDGQVKDV
                     RKQFRSNLFEIEYKGTSVGFANALWTGFELVETRAQDDHAFAVIKNLNNSSTNDLLRQ
                     LLNEVEVVSFRERIPTMNDIFIESVQNNKPVEA"
     gene            21059..23143
                     /locus_tag="PKU82_02780"
     CDS             21059..23143
                     /locus_tag="PKU82_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ81828.1"
                     /translation="MRLLSFVLCFMFCLNVSVWAQQAESDSLQHLLRTQADDTSKVNT
                     LNELSYVYYQTAVYDSELYYAQQALNLAQKLNWKRGMAVSLKNIGSACDDMGNNSDAL
                     VYYGRALNIFSSIDDKSGVSACYNNMGLVYRTLGNFSEALKNHFASLKIKEAIGDKNG
                     IALSYNNIGVVFNAQKNYDEALKNHRAALAIRLSLGNKRGIASSYGNIGNVYQAQGNN
                     TEALKNYTEALKIAEETGDKVGMTTALNNMGAAYFEMGDYENALQHNTDALRIAEEAG
                     DLSSIAAASINTGNVMVQMKQPQAATEWLQKALEIGKEIGEVEVIKNSYEGLSQADSA
                     LGKYSDALQHYKMFIRYRDSIVNEENTLKTVQLQMQYEYDKKDAIARAVQEKKDEVAQ
                     QEVQKQKLLRNSFIAGFALMLALAAVSYRSFVRKRKDNHLIEKQKLLVEEKNKEITQS
                     LHYARHIQAAVLPNQKNIAALLKNHFILYLPKDIVSGDFYSVLQNKDKVIVAAADCTG
                     HGVAGAFMSMIGTSLINHLVNEKNITNPAVILDELNEGIINSLKQRQGDSNDGMDIGL
                     CSIDFKENKLLFAGANRPLWLFRNEEIKVIKPDKFPIGGMQVDHKNKYTAHSIDLLPN
                     DTIYIFSDGYSDQFGGEQGKKMMSKRFKQYLQVMQHLPMDKQKEQLEQLFNSWKGNHE
                     QVDDVLVVGIRV"
     gene            23264..>26208
                     /locus_tag="PKU82_02785"
     CDS             23264..>26208
                     /locus_tag="PKU82_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81829.1"
                     /translation="MFKNLFFSKGVKSIALAAFCLLTFTLGANAQILAWEFNGAAGNE
                     TTIAATTLDANLNSSSVSRGSGVNASGLTNAFASTDYTVSGTFANAVTNNEYQQFSVN
                     AKSGYKVSLSTLDVNFRRSNTGPNTFQWQYSLDGFATAGINIGSAISYTTSPTNGTAQ
                     SQIDLSVISALQNVSNATTITIRLYGYGASAGTGTFALGRLTGNDLAIGGTVDPDGGS
                     PVLTVGTLSNQFGNVCIGTTTNYETFTVSGTDLDGSSVDVGTLSGFTYCLTPGGTYTS
                     TLNLPYTAPTLSSTTVYVKFSPTLVQSYAGSIPVTGGGASAGSCSVSSASGINTSPSL
                     TTGSATGITQTTATCEGTITDNGCTSVSAYGIEYSTTNNFTPGTGTQVSSSNISGNTY
                     TSALTGLAGNTTYYYRAYATNTAGTGYGAQASFTTDAFTAPVATAATNIGIHSFRANW
                     NSVSGATGYFLDVSTSSTFLAPVTTDLVYWDFPNDPDDATADGGILSNLSATISSTAG
                     GTMTFSTNGASTKSASINNWDNGNGTKYWEVSFDATGYYNLAFSSKQQSSNTGPRDFK
                     VQYKIGSGGTWTDVTGSSVTVANNFTSGVLTDIALPAACDNQSSVYLRWIMTSNTSVG
                     GGSVAATGTNRIDDLFVTGYEGDFVLENQSVAGLYYDVTGLNPITTYYYRVRATDGVA
                     TSDNSNVINLTTLDVCDLFTVTASSNAPICSSTNLNLNANASGSDGTITGYEWSGPGG
                     YSNMTQNPSVSNPLAGTYTVTVTDDNGCTVTDNVSATVEAAPTANAGSDDIICSSGSA
                     ALNGIIGGDATGGTWTTAGDGTFSPNATTLNAAYVPGTTDISNGSVVLTLTSTGSVLC
                     SAVNDQVTITIQTGVPSQPAVVSGAPTSVCPPAGPYTLTTSSTNANSYNWYVVGANTG
                     ITFLSANGSNSMDVQYGVTANSSYSVRVEASNACGTSSYRSAFTRRSVSTPATVSGNK
                     VACATTSDT"
BASE COUNT         8067 a         5014 c         4997 g         8130 t
ORIGIN      
        1 ataagaatac cgatataggt gccatcaact cacgcgagca attgaataaa ataaaagcat
       61 tggttaaagt aggtgttaac gaaggcggtg actgttatca gtcatcttgc acattgccgt
      121 caaaaggttt ttggtttaag cctacaatat ttaccggtgt tgcacagtca agtagaattg
      181 ttcaggaaga aatatttggg cctgttcttt caattcaaac attcagaaca gtagaagagg
      241 cagttgaaaa agcaaacaac acaccttatg gtttaagtgg aggcgtatgg tctgataaag
      301 gcagtaaggt atttaaagtt acttcaaaaa taaaagcagg tgtattatgg gccaacactt
      361 ataataagtt tgatcctact tcgccatttg gaggatttaa agaaagcggc atgggtcgcg
      421 aaggtggcat gcatggactt agtgcatatt taaaataatt ttaacgcatt aacattatgg
      481 agacgaaaca aaaagagaaa ataaaaaaag tagcagttgg acttaatggt cacagccatc
      541 atgcagaaac cgaaataagg ttagctgtac aaaaaactta taagctttac attgatggta
      601 aatttccacg aacagagagc ggaagatatt ataagcttaa tgataagaac ggaaaggtaa
      661 ttgctaatat ttgtcgtgca tcaagaaaag atttcagaga tgcagttgta gttgcacgaa
      721 aagcacaagg cgactggcaa aagaaatcgg catttaatag aagtcagatt ttgtatcgca
      781 ttgcagaaac tctggaaaca cgaagactac aatttgtgtc aaccttaggt ctgcaaggtg
      841 tgagtgaaac ggcagcacaa aaggaagtga gtgcagcagt agatatgctt gtttattatg
      901 caggatggtg cgataaatat cagcaggttt tttcaagtgt gaatcccgta gaatcatcac
      961 attttaattt ttcttttcct gagccaacag gagttgttac agcaattgca tcgccagaac
     1021 aaggtttatt aaatctggtt tctcttatgg caccttcact tgcaggaggt aattcaataa
     1081 ttattctggc agcagaaaaa tttcctctca cagcaattga ttttgcagag gtgcttaatg
     1141 caagcgatgt gcccggtggt gttgtaaata tacttacagg ttatagtaaa gaattgcata
     1201 cgcacttttc gtcacacatg gatgttaatg ccattgttta tgcaggtgat aacagtgctg
     1261 aattgaagac catacaaacc aatgcagcag gtaatgttaa acgcgtagta gtagttcaga
     1321 atattgctga agaaagtcca tatcgcatca tggaacttca ggaagtaaaa accacatggc
     1381 atcctgtggg tatttgagct tgggtcaata tatatttttg tattcatgaa tgcaatatct
     1441 ttgacctgaa cgaaagccaa ggctatcgtt tagctgtgaa atgatcaaat attaattggc
     1501 aaaattgaag tcaagtgctg tcttctgaca gaagtgattt atttgaattg taaacacatt
     1561 tatgaaaaca ttttttaatt tgattttttt cttgtttgta ggaatgaatc tgaaagcaca
     1621 aacttatggt tgtacagatt cgttagccaa taattataat gccaatgcaa ctgcaaatga
     1681 tggtagttgt atttataact cagccaatgt tgttccattg agtagtaatt tattgccaat
     1741 tgcattagac gaaacatcag gattaacttt ttggaataat atgttgtggt cgctgaatga
     1801 tgatgcagat aataatttat atgcaattga caccggaaat tttaataact atacagctta
     1861 tcctttaaac gagcctaata ttgactggga ggaaatttcg caggatgatg actattttta
     1921 tataggtgat tttggaaata atgtcagcgg aaacagaacc gatttgaaaa tattgaaaat
     1981 cgaaaaaaac tctttactgc agaacaatcc taatgttgat acaataaact tttcttattc
     2041 aacgcaaaca aattttaatc ctactgccat aaacagcacc gattttgatt gtgaagcttt
     2101 tgtaataaca gccgacagta tttatttgtt tacaaaagag tggataagca atcattgctc
     2161 tgtttattct cttccaaaca agtcgggtag ttatagtgca aattttattt catcctataa
     2221 tgtgaacgga ctcgttaccg gtgctaccca aattcaaaat agtagggcag ttgtactttc
     2281 aggctattca acagtgatgc aaccttttgt tttactttta tatgattatc agggattcaa
     2341 tttttttagt ggtaataaaa gaattatcaa tgttaatctg ccttttcatc agactgaagg
     2401 catagcaact gttaatggat taaaatatta tttgtccaat gaaaaacttg tacaaacacc
     2461 aataaacatt caggcaaaga ttcatgaaat agatttaacg ccatatttaa gtcactattt
     2521 gaattctgtt tccggaagtt ttgatatatc tgaaaatcct tatgcaataa atatttaccc
     2581 aaatcctaca acagatttta ttaatgttaa atggaataca ggagtgaaag ttgcacgaat
     2641 taaaattttt aacatcagcg gaaaagaggt gatgaaccaa atagtaacag aaaagtattt
     2701 tcagattgac atcagaagtt ttgaaaaggg aatttatttt ctgagtgtta atgatagcgt
     2761 attccggaaa gtagtcaaaa tgtgactgta ttgaaaaaca gattgtagtt cgttgacttt
     2821 atattactgg tttgtgtatt ttagcttgca gttaaatcaa tagacatatt atctttgttt
     2881 taactttaca acacctaaca atctaaatta aattttatga aagtcatttc tgcagatgac
     2941 aagagtgcaa catcgctttc cgatagtgcg caacttcgat atattacctt cattttattg
     3001 tatttctcac aaggcattcc agaggggctg acactttttg ctatccctgc atggctggct
     3061 ttcaatggta agagtgctgt tgaaattgca gcatatagtg caactattat gattccgttt
     3121 agtttaaaaa tattgctggc tccaatcata gaaaaataca cctatctgcc tatgggacgc
     3181 agaagacctt ggttgttgtt tggtcagttt ggaattatgt caggactaat tgcacttgct
     3241 tttgtgcccg agccactgaa taatattttc gttataattt gtgtagttct atgtatgcat
     3301 gtttttgtta tgtttcagga tattgctaca gatagcctag tgatagacat tgttccggtt
     3361 gatcagcagg gaagagccaa tagtttaatg tggggttcca aaacaattgg atcttctgtg
     3421 tcactttttt taggaagttg gctcatcaat gaatatggtt ttgcaagtgc ttttgtaatt
     3481 ttatcggtgc caattttagt tgtcatgttt gtgccacttt tgttgcggga acgtaaagga
     3541 gagaaacttc ttccttggac tcatggccaa acttctgatg aggcaaaaca atttgtagtt
     3601 gaaagctgga agaagatgtt taagtcattc aagcaagttg ttttgttgcg taattctatt
     3661 ttgttgttgt cttctgtttt tattgcactg gctgcaatat attttatcaa tacattactg
     3721 ccaattttta caatacagaa acttggctgg accaatgtgt atttctcaaa aatattttca
     3781 tcgtgcagat taatcggagg tgtatccgga atgttggttg gcgcacttgt aattcagcga
     3841 atgggtgtta tacgttttat acaaagtagt ttaatattaa tgattgtaat tgctattcta
     3901 tttgttatgg gtgctccttt ttggcatcat cttaatatga taattggatt tatcactacc
     3961 ttttgcatat tggcaacatt aattaatatt ggtgtgcttg cattagccat gcaactgtgc
     4021 tggaaaagag tttctgccat tcaatttaca ttttgtatga caatatttaa tgccgggttt
     4081 gcaacgggtg ctgcactgct gggatggctg aagccttaca tggactggca acaactgttt
     4141 ttggtttttg cctgcttgat agcttttaca atgttgttgt ttaaatttat gaaaacatca
     4201 aaccatgtaa aacaagttga aaaattagaa gaccagtata ttgatgtttt aaaagcagaa
     4261 gggagtttgc tggtaaaatc agagacagtt tagtttacaa aaaattaatt tttaatcgaa
     4321 aacaccttac ggaaataaaa ttccgggaaa cgaatttgca tctgtttgtg gaatgcgtgt
     4381 ctgatatttt tcgttaatag cttttataaa ttcattggca ataagtgcac tgcctcttcc
     4441 agtaggcgta atgccgtcaa gagaaaaaaa ctgtccttta agaaactcgc tgctgtaagt
     4501 tacaccattg tgcggaaaac cattgttgat ttttttaaag aaggcataca tatcaacata
     4561 agctaaattg ttttcatcag cttttgtttt aagataattg ttgtaggcgg tgattgtatt
     4621 tctaagcgta ttgatttctg aagtggtgag cacatatctg tttccaagag gcttctgtgt
     4681 attgctgccc caacctgcac agtttatact gtcaagggta accggcaaca gcactaactc
     4741 acccggcttc atttgtcttt ttcctgatgc tgcagatggg tcagcaatta ccaatggatt
     4801 gttacctgcc tgaaaagtta tcggtggatt gtttgaagca tataatgcat taagatcctg
     4861 agcctgttgt tctgtaagta ccagagcatc gtaggctatg gaagtaaaat agggtaggtc
     4921 gagtaggtca ggaatatttg caactacacc tttgcgattt cctgcaatga gtgatgtaag
     4981 agcattattg atgttatttt gaaaattagc taatggtgta atggtagcca atgtctgatt
     5041 gctgcctgcc attgcacaat cataaatgtc gttaaagcca acccaaaaag aaaagaacgt
     5101 gggtagttgt ctgactgcat ctgtaagtac tgttgatgtt tcagggttgg tagcaaatcg
     5161 tgcgtagaaa ggattttttg aaagcagtaa atctgtagag ccaaaattgg cattgtctaa
     5221 atcagacgat ttcatacccg gcacacccat gttgttgtaa ggtgcattgt tgccgatgta
     5281 agtttctact gcgacaatgt cagttacgtt ggatggaaat gtagagaagc ccggagcaga
     5341 actgcatgtt gtgccatagc caagttttgt ctgtggaata taggtctgaa gaaaaaagtt
     5401 gaaccctgca ccatcatttc cgctaaccaa tggttgcaga aactcgccac cgccaacttt
     5461 ttttaattgt gaggcaagta tggctgcaaa actattttgt tgccccatgt caaacaatgc
     5521 attgttctga tagcccgctg tgtaagaact gccaatagca acatacttcg aaaagtcaat
     5581 actgttgttg tcgaacttca gctctttcaa ctcaggcttg caacctgcaa tcatgattac
     5641 tgcaagaagt aaacttttca gaatatgatt attgctcatg cgtttatatt gtggtttatt
     5701 atttaattaa aatgaatagt tgacaccaac accaaaacaa tgcgacagtg cattgaatgc
     5761 accattcaat ccggtatctt tatttacacc ttcgcgctcg gccttagttt caaaattgta
     5821 tgacccttca ataacaaaag ttttactcac gttaaatgat gcaccaatac caaaaacata
     5881 tctgtcgcca tcgggcatat caggggtcat gtagttttct tttaccggag tctgatcgaa
     5941 ggcaatacct gcacgacctg taactttatc gctgatttta taattcgcac ctaatctaag
     6001 tgttaatgca tctctgtatt tgcgtgcctg acgaaattct tgatttaatg ctgtgtttgg
     6061 tgcatcaaat tcataattca gagaatcgaa tgacgaccaa ccgttgaggt tagattcaaa
     6121 actaagtttt aatcctttaa tgacttcata gctgaaacca attgacaaaa cagaaggcag
     6181 cggcatttct gcagcggcat ttgctgaggc aggatattga ttcgttaacg atgccggaat
     6241 attactaaag gtaacatcag tgtttttaaa gttgagttta acttttgacc ggtatgcaag
     6301 tgcagcagtt aattttttat tgatggcata atttattcct gcattaaaac caaagcctgt
     6361 tgccttacca tcaagttgtg catctgctcc atctgcaact atcaacgcac gattccattt
     6421 atagttggca ctggcaaaaa caaagcctgc accaattcct aatttttcag aaattttata
     6481 agagaatgta ggttgaataa atatggtgtt cagcttagct gacagtataa tatttcgccc
     6541 ttcccaatcg cttttccagg ttgtgttgta tccaaaaggc gtattgacta caatacctgc
     6601 agccattttt tcagataaca ttatggtgcc acctacataa aacggcagca tattttttga
     6661 ttctgtacgg tttaccaatc cgctgttgtt actacggaaa gccattcttg actgcgaata
     6721 agaaatacca ccaaagacat tacttttctc aagaaaaacc atgccaccgg gattgtaaaa
     6781 tataactgac ccatcagttt gcaatgcaat cactgcaccg gccatacctg ctgaactatg
     6841 gctttgtggt tgcatctgaa aaccaccgcc tgatacatga agactacaga aaagaaacag
     6901 aattgaaaaa cctgaaatga attttttcat attaatttta acgcaaacaa aaataagaac
     6961 agcaagctta gtaagcaaag atatacataa cgatgacaat ttgttgttaa caccgatttg
     7021 ttggtgcagt ttctaaaaaa tgtttgcact ccaacaacca atattcggat tttttgtatt
     7081 aaaattgcac tgtaattgta atacattttt tcaccaacca aaatcatttc acatgttagt
     7141 caagaagtta tttctgatat tattttctgt tgcaacaact ttaacttgtg cagcatctgt
     7201 tggcgacaca acctttgtaa gctcgcatac caatgacact gtagtaacca atcccaacac
     7261 aggtagcaat ggttatccaa attgggtagt atttccttct gcatccacca actatcgtaa
     7321 gataatttta actttaacac atcgttgccc aagccctatg tcatgtggcg aatgggatta
     7381 tctcgattat atttacatca gacgtacagg aggtcagaac aatccctctc aggatattga
     7441 actggcacgt tatataacac cttatggcaa tacattcaac tctacattca agtcgtcatg
     7501 gacgcttgat gtaacagatt ttgcatcatt ccttcacgac tctgtagaga tagaatatat
     7561 ccacaccggc tacgaaacca atgtgggtcg cggatggctc gttagtgttg attttgcatt
     7621 gatagaaggt actccggtga tgaatcctat tgcatttaaa agattgtggc atggcagttt
     7681 tccttatggc aatgcatcca atccaattga aaactatctc ggtgccgaca ctgtgacgct
     7741 taattttaat actgcaaatt taagactgaa tgttacccaa tccggacatg gtgccgatgc
     7801 caattattgt gcagaatttt gctcaaaaac aagaactatt tattttgata attcattggt
     7861 agataccaag caagtttggc ggttgtgtgg aacaaaccct atttatccgc aaggaggcac
     7921 atgggtttac gaccgtgcaa attggtgtcc cggtaactgg gtctatcctg ctgtcaataa
     7981 ttttgctgtt gccggaggaa gcacgcacat tattgacatg aatatgcaga actataacgt
     8041 aacaagccct tccgctaatt acgtcatcga aggacagctt tttgaatttg gtccattgaa
     8101 caacaatata gatgctgcca tagatgaaat tcttgcacct accgataagt ttgaattttc
     8161 gcgcagcaac cctacctgca acaatccaaa aatcagaata aaaaacaatg gctcaactgc
     8221 aataacttct gcaacaataa aatatggatt agtaggcgaa cccgaaagta cattcaactg
     8281 gacaggctca ataaatacat ttgcaacagc agatgttgac ttacccggtt taatcagcta
     8341 tacaagtggc aacagaattt ttaaagcata cattgctgac gttaacaata cggcagatca
     8401 gtatgtttat gatgatacag cttattcaaa tgcctatgtt cctgaagtga tggacactgt
     8461 atttatctta ttggtaaaaa caaataacta tcctacggaa aatggctata aactttatga
     8521 tgaaaataat aatatcatcg tacaacgact accgggctca atggctgcca acacaattta
     8581 tcgtgacacc attcatgcaa ctcccggttg ttatcgttat gaaataacag atagtggcga
     8641 tgatggttta tactggtggg caaactcagc acagggaaca ggttaccatc gtattctgcg
     8701 attgaacaat agcattgcaa gaaatttcga aagagatttt ggctcacgtt acagctatcg
     8761 ttttgtttct gctccgggtg ccgttgttgg catcaacaat caggagcgct atgctgatat
     8821 agtagtttat cctaatccaa ccaaaggaat catcaatgca gatattacat taacttcggc
     8881 aagcccgtta gaggtgtctg tttataatcc tttaggtcag cgtgtggctg ttcaaaacag
     8941 aacaaatttt aatgcaggaa taataacttt cgatctggag aatcaaccaa agggagttta
     9001 tttggttaaa gttgttgcta acgatcaggt taaagtgcaa cgtgttgtac tggagtaaat
     9061 aacaatgaaa tttaatttta cagcaggtag tttagttata taagcacctg ctgtaacttt
     9121 tagtaacagg cctgcgtcat acaataaaat gaaggatatg aaaagatgta atcaatttag
     9181 tctgaccttg tttttaatgt tgtttgcact ttttgcaaat gcacaaaaac ataaaacttt
     9241 agatcagttt acagacatag acttgcgtgg atcgcttaat gcagtttata aattggacag
     9301 tacatggtca attgaagtgc ctgaagataa tgtcaccaca cagtggagtc ttgaaaataa
     9361 aacccttgtg ctaagtggtg caggaagtaa tataattgtc agaagtcctg atttgcaaaa
     9421 gataaatatt tccggttcgg gaagtatttc tgcagaagat actatggaat tgcacgactt
     9481 gaatatagat atttcaggta gtggaaaatt taagatgaac tatgtaaaag caaagcacat
     9541 tgaggcaaac atgcgtggtt caggaaaaat aattcttgga ggttctgcac aatcattaaa
     9601 tgcaaatctt gccggctcag gaaaaattat tacgcataaa ttaaatgttg atcgggcaga
     9661 agtaaatgtc agcggcagtg gcacatcatc agtttgggtt agcgattcat tatcagccaa
     9721 cattgctggc tcaggaaatc tgtattatgt ttcttcgcca agaacaatta atcaggccat
     9781 tgcaggctcc ggaaaaattg aacctataac ttctgcaatt gaatcggaac tgaactcaaa
     9841 cagtattaca atttctggaa gcaataaatc cggtacttat tggtcgggat ttgaattggg
     9901 atttaatgga ctttataaca gtaaatcaaa attcgatact cctgatgggt atgatttttt
     9961 agagatgatt cctgaaaagt ccttagcagt aaacatcaac ttttatgagt acgatgtaaa
    10021 actgattaag cgttatgtca ttgcttcaac aggtattggt ttatcataca ataattatcg
    10081 tttcagaaaa aatatgagcc tgattcctga taccaatgca ttgctttata ctcaggacac
    10141 catgaacctg cgtaagaata aacttactgt aagttatgcc actgttccag ttctgcttac
    10201 tttcaacaca agttcaaatc ttaaaaaagc ttttcacatt actaccggtt tgttgtttag
    10261 ttacaaattg ggctcacata ctaaaaaggt ttacaataaa gatggtaaaa aagagaagga
    10321 taaaactttt gatgatttta atattgatcc cttccgttat gatgcaacag ttcgcatagg
    10381 ctacagtgat tttactgtat ttgcaaacta tgcactgtca gaacttttca agagtggaaa
    10441 ggggccggag ttgcatccct ggactattgg tgtttcacta gtaccttggt agtactaaag
    10501 cctgtgtcga gttgacaaag taggttgtaa tcagaattga taaatgaatt atgtgggcgt
    10561 gaattatttc acgcctacat agtttataac atcatcctta gtgatggttt tgccaccgag
    10621 aattaccaaa cgctctacca cattacgcag ctcacgaata tttcctgtcc agtttttttt
    10681 ctgcaactgc tctattgcat ccggagcaat ttcttttgca gggcttttat attcatcagc
    10741 aatttctttc agaaaataat ttgtcagaac cggaatatct tctctgcggt cgtccaatgc
    10801 aggcacatga atgagtataa cactcaaacg atgatataaa tcttctctga aattattttt
    10861 ttctatttct gattttaaat ctttgttggt agcagcaata atacgtacat caaccggaaa
    10921 atctttttcg ccacctactc ttgtaattct gttttcctgc aatgctctca acactttggc
    10981 ttgtgccgac aggctcatgt cgccaatctc atcaagaaaa atagtgccac cgtttgcttg
    11041 ttcaaatttt ccgcttcgtg tttttatggc agaggtaaat gcgccttttt catgaccaaa
    11101 caattcgctt tctattagct ctgatggtat tgctgcgcaa ttaacttcta tcagtggcat
    11161 gttgtttctg ctgctctttt catgtatcca tcgtgccacc aattcttttc ctgttccatt
    11221 gcttcctgta atcatcacgc gtgcatcggt aggtgccaca cggtcaatca tgtcttttat
    11281 tttacgaatg ccttcacttt caccaatcat ttcattggta cgcgaaattt ttcttttcag
    11341 tgtcttggtt tcttttacca gtactgtacg gtcgagtgca ttgcgaacag taatgagaat
    11401 acgatttagg tcaagcggct ttgcaataaa atcaaaagca cctttacgaa tggcttcaac
    11461 agcagtgtca atgttgccat gtcccgatat catcaccaca ggcacttcag gacatttatt
    11521 ttgtaaattc tccaacactt caatgccatc catgcgtggc atttttatgt cacaaaaaat
    11581 aatatcatat ttttctttct cagctctttc aactcaggct tgcaacctgc aatcatgatt
    11641 actgcaagaa gtaaactttt cagaatatga ttattgctca tgcgtttata ttgtggttta
    11701 ttatttaatt aaaatgaata gttgacacca acaccaaaac aatgcgacag tgcattgaat
    11761 gcaccattca atccggtatc tttatttaca ccttcgcgct cggccttagt ttcaaaattg
    11821 tatgaccctt caataacaaa agttttactc acgttaaatg atgcaccaat accaaaaaca
    11881 tatctgtcgc catcgggcat atcaggggtc atgtagtttt cttttaccgg agtctgatcg
    11941 aaggcaatac ctgcacgacc tgtaacttta tcgctgattt tataattcgc acctaatcta
    12001 agtgttaatg catctctgta tttgcgtgcc tgacgaaatt cttgatttaa tgctgtgttt
    12061 ggtgcatcaa attcataatt cagagaatcg aatgacgacc aaccgttgag gttagattca
    12121 aaactaagtt ttaatccttt aatgacttca tagctgaaac caattgacaa aacagaaggc
    12181 agcggcattt ctgcagcggc atttgctgag gcaggatatt gattcgttaa cgatgccgga
    12241 atattactaa aggtaacatc agtgttttta aagttgagtt taacttttga ccggtatgca
    12301 agtgcagcag ttaatttttt attgatggca taatttattc ctgcattaaa accaaagcct
    12361 gttgccttac catcaagttg tgcatctgct ccatctgcaa ctatcaacgc acgattccat
    12421 ttatagttgg cactggcaaa aacaaagcct gcaccaattc ctaatttttc agaaatttta
    12481 taagagaatg taggttgaat aaatatggtg ttcagcttag ctgacagtat aatatttcgc
    12541 ccttcccaat cgcttttcca ggttgtgttg tatccaaaag gcgtattgac tacaatacct
    12601 gcagccattt tttcagataa cattatggtg ccacctacat aaaacggcag catatttttt
    12661 gattctgtac ggtttaccaa tccgctgttg ttactacgga aagccattct tgactgcgaa
    12721 taagaaatac caccaaagac attacttttc tcaagaaaaa ccatgccacc gggattgtaa
    12781 aatataactg acccatcagt ttgcaatgca atcactgcac cggccatacc tgctgaacta
    12841 tggctttgtg gttgcatctg aaaaccaccg cctgatacat gaagactaca gaaaagaaac
    12901 agaattgaaa aacctgaaat gaattttttc atattaattt taacgcaaac aaaaataaga
    12961 acagcaagct tagtaagcaa agatatacat aacgatgaca atttgttgtt aacaccgatt
    13021 tgttggtgca gtttctaaaa aatgtttgca ctccaacaac caatattcgg attttttgta
    13081 ttaaaattgc actgtaattg taatacattt tttcaccaac caaaatcatt tcacatgtta
    13141 gtcaagaagt tatttctgat attattttct gttgcaacaa ctttaacttg tgcagcatct
    13201 gttggcgaca caacctttgt aagctcgcat accaatgaca ctgtagtaac caatcccaac
    13261 acaggtagca atggttatcc aaattgggta gtatttcctt ctgcatccac caactatcgt
    13321 aagataattt taactttaac acatcgttgc ccaagcccta tgtcatgtgg cgaatgggat
    13381 tatctcgatt atatttacat cagacgtaca ggaggtcaga acaatccctc tcaggatatt
    13441 gaactggcac gttatataac accttatggc aatacattca actctacatt caagtcgtca
    13501 tggacgcttg atgtaacaga ttttgcatca ttccttcacg actctgtaga gatagaatat
    13561 atccacaccg gctacgaaac caatgtgggt cgcggatggc tcgttagtgt tgattttgca
    13621 ttgatagaag gtactccggt gatgaatcct attgcattta aaagattgtg gcatggcagt
    13681 tttccttatg gcaatgcatc caatccaatt gaaaactatc tcggtgccga cactgtgacg
    13741 cttaatttta atactgcaaa tttaagactg aatgttaccc aatccggaca tggtgccgat
    13801 gccaattatt gtgcagaatt ttgctcaaaa acaagaacta tttattttga taattcattg
    13861 gtagatacca agcaagtttg gcggttgtgt ggaacaaacc ctatttatcc gcaaggaggc
    13921 acatgggttt acgaccgtgc aaattggtgt cccggtaact gggtctatcc tgctgtcaat
    13981 aattttgctg ttgccggagg aagcacgcac attattgaca tgaatatgca gaactataac
    14041 gtaacaagcc cttccgctaa ttacgtcatc gaaggacagc tttttgaatt tggtccattg
    14101 aacaacaata tagatgctgc catagatgaa attcttgcac ctaccgataa gtttgaattt
    14161 tcgcgcagca accctacctg caacaatcca aaaatcagaa taaaaaacaa tggctcaact
    14221 gcaataactt ctgcaacaat aaaatatgga ttagtaggcg aacccgaaag tacattcaac
    14281 tggacaggct caataaatac atttgcaaca gcagatgttg acttacccgg tttaatcagc
    14341 tatacaagtg gcaacagaat ttttaaagca tacattgctg acgttaacaa tacggcagat
    14401 cagtatgttt atgatgatac agcttattca aatgcctatg ttcctgaagt gatggacact
    14461 gtatttatct tattggtaaa aacaaataac tatcctacgg aaaatggcta taaactttat
    14521 gatgaaaata ataatatcat cgtacaacga ctaccgggct caatggctgc caacacaatt
    14581 tatcgtgaca ccattcatgc aactcccggt tgttatcgtt atgaaataac agatagtggc
    14641 gatgatggtt tatactggtg ggcaaactca gcacagggaa caggttacca tcgtattctg
    14701 cgattgaaca atagcattgc aagaaatttc gaaagagatt ttggctcacg ttacagctat
    14761 cgttttgttt ctgctccggg tgccgttgtt ggcatcaaca atcaggagcg ctatgctgat
    14821 atagtagttt atcctaatcc aaccaaagga atcatcaatg cagatattac attaacttcg
    14881 gcaagcccgt tagaggtgtc tgtttataat cctttaggtc agcgtgtggc tgttcaaaac
    14941 agaacaaatt ttaatgcagg aataataact ttcgatctgg agaatcaacc aaagggagtt
    15001 tatttggtta aagttgttgc taacgatcag gttaaagtgc aacgtgttgt actggagtaa
    15061 ataacaatga aatttaattt tacagcaggt agtttagtta tataagcacc tgctgtaact
    15121 tttagtaaca ggcctgcgtc atacaataaa atgaaggata tgaaaagatg taatcaattt
    15181 agtctgacct tgtttttaat gttgtttgca ctttttgcaa atgcacaaaa acataaaact
    15241 ttagatcagt ttacagacat agacttgcgt ggatcgctta atgcagttta taaattggac
    15301 agtacatggt caattgaagt gcctgaagat aatgtcacca cacagtggag tcttgaaaat
    15361 aaaacccttg tgctaagtgg tgcaggaagt aatataattg tcagaagtcc tgatttgcaa
    15421 aagataaata tttccggttc gggaagtatt tctgcagaag atactatgga attgcacgac
    15481 ttgaatatag atatttcagg tagtggaaaa tttaagatga actatgtaaa agcaaagcac
    15541 attgaggcaa acatgcgtgg ttcaggaaaa ataattcttg gaggttctgc acaatcatta
    15601 aatgcaaatc ttgccggctc aggaaaaatt attacgcata aattaaatgt tgatcgggca
    15661 gaagtaaatg tcagcggcag tggcacatca tcagtttggg ttagcgattc attatcagcc
    15721 aacattgctg gctcaggaaa tctgtattat gtttcttcgc caagaacaat taatcaggcc
    15781 attgcaggct ccggaaaaat tgaacctata acttctgcaa ttgaatcgga actgaactca
    15841 aacagtatta caatttctgg aagcaataaa tccggtactt attggtcggg atttgaattg
    15901 ggatttaatg gactttataa cagtaaatca aaattcgata ctcctgatgg gtatgatttt
    15961 ttagagatga ttcctgaaaa gtccttagca gtaaacatca acttttatga gtacgatgta
    16021 aaactgatta agcgttatgt cattgcttca acaggtattg gtttatcata caataattat
    16081 cgtttcagaa aaaatatgag cctgattcct gataccaatg cattgcttta tactcaggac
    16141 accatgaacc tgcgtaagaa taaacttact gtaagttatg ccactgttcc agttctgctt
    16201 actttcaaca caagttcaaa tcttaaaaaa gcttttcaca ttactaccgg tttgttgttt
    16261 agttacaaat tgggctcaca tactaaaaag gtttacaata aagatggtaa aaaagagaag
    16321 gataaaactt ttgatgattt taatattgat cccttccgtt atgatgcaac agttcgcata
    16381 ggctacagtg attttactgt atttgcaaac tatgcactgt cagaactttt caagagtgga
    16441 aaggggccgg agttgcatcc ctggactatt ggtgtttcac tagtaccttg gtagtactaa
    16501 agcctgtgtc gagttgacaa agtaggttgt aatcagaatt gataaatgaa ttatgtgggc
    16561 gtgaattatt tcacgcctac atagtttata acatcatcct tagtgatggt tttgccaccg
    16621 agaattacca aacgctctac cacattacgc agctcacgaa tatttcctgt ccagtttttt
    16681 ttctgcaact gctctattgc atccggagca atttcttttg cagggctttt atattcatca
    16741 gcaatttctt tcagaaaata atttgtcaga accggaatat cttctctgcg gtcgtccaat
    16801 gcaggcacat gaatgagtat aacactcaaa cgatgatata aatcttctct gaaattattt
    16861 ttttctattt ctgattttaa atctttgttg gtagcagcaa taatacgtac atcaaccgga
    16921 aaatcttttt cgccacctac tcttgtaatt ctgttttcct gcaatgctct caacactttg
    16981 gcttgtgccg acaggctcat gtcgccaatc tcatcaagaa aaatagtgcc accgtttgct
    17041 tgttcaaatt ttccgcttcg tgtttttatg gcagaggtaa atgcgccttt ttcatgacca
    17101 aacaattcgc tttctattag ctctgatggt attgctgcgc aattaacttc tatcagtggc
    17161 atgttgtttc tgctgctctt ttcatgtatc catcgtgcca ccaattcttt tcctgttcca
    17221 ttgcttcctg taatcatcac gcgtgcatcg gtaggtgcca cacggtcaat catgtctttt
    17281 attttacgaa tgccttcact ttcaccaatc atttcattgg tacgcgaaat ttttcttttc
    17341 agtgtcttgg tttcttttac cagtactgta cggtcgagtg cattgcgaac agtaatgaga
    17401 atacgattta ggtcaagcgg ctttgcaata aaatcaaaag cacctttacg aatggcttca
    17461 acagcagtgt caatgttgcc atgtcccgat atcatcacca caggcacttc aggacattta
    17521 ttttgtaaat tctccaacac ttcaatgcca tccatgcgtg gcatttttat gtcacaaaaa
    17581 ataatatcat atttttcttt ctcagccatc ttaatgcctt caataccatc gggcgcttca
    17641 tccactttgt aattctcgta ttcaagaatc tctttcagcg aatggcggat gcttttctca
    17701 tcatctataa ttaatatacg tgacatgctc ttttgtttac ggtcagtttg ctgttgttat
    17761 ttattcattg caaaataaag cattccttcc tttaatgcat aagcactcac taaaagttta
    17821 ttcatattgt gtttgtgaat aacatagtga agtaacatag atgcaacaac aatcatatcg
    17881 gcacgcatgg caatcattcc cggcatttca agtcgttgtt gtaaggttga ggttaacaaa
    17941 atttgatgta catcaaaata ttcctctgag tccaatggcc accaggtagt gtttgctttt
    18001 attttatgaa aacaatgaag acataaatcc agaaaggtat cgaaagaacc tgccgatcct
    18061 accaatgtat ttaccggacc atattgtttt aatgcttctg taagtggaaa taaaactgag
    18121 ttgatatgtt cagtaattat tttttcctgc acttcatgaa tcgggtctga tggtttaaac
    18181 atatctaaca acagggcagc acccaaagga aaacttttcc gccacaacat ttttccgttt
    18241 cctgcaatta caaattctgt acttcctcca ccaatatcca ttatcagtac tttttctttt
    18301 ccaaaaggaa cagcatgact tactcctgag tatatcagtt ctgcttcgcg caagccatca
    18361 ataatttcaa gtgtaatgcc tgattgtttt tttgcttctt caacaaaatc ttttccattg
    18421 gctgctctgc gtatggcttc agttgcataa cacaattgtt tgtcaatgat aaattgtttc
    18481 atgtcggcag caaattcttt aagtgcgtca atgccacgac cgaaggctgc acgtgctatt
    18541 ttattattgc tgatgccgcc ttcgccaagt tttacagcta agtttttgcg gtaaagaatt
    18601 ttaaaactgc cttctgaaat accggcaata atcagatgga aggtgttggt gccacagtca
    18661 attacagcaa tgttcattga cattacttat aaaacaacca gcgaaacaat tctttgtagg
    18721 taggcttttt gccatacatc aatattcctg tacggtaaat ttttgcagct atccacgtag
    18781 tgaaaataaa acctgccact aacaacacca tcgaaattac aatatcaatt ataggtgggt
    18841 caaatggcat acgcaccatc attacaatgg gcgatgtgaa tggtattatt gaaaaccaat
    18901 agcccaattc actttcggga ttggtcatca caatagtcga aactacataa gcaaatatga
    18961 gtggaatggt aatcggcaac ataaattgtt gcgtgtctgt ttctgcatct actgcagcac
    19021 caatggcagc aaacaaggca ctgtaaagta aatatccgcc taagaaataa aatacaaaac
    19081 acaacagaat ttttgtgatg ttaaatcctt caaatatttt cataaaatcc atactggcag
    19141 gttgcatttt ttcctgcgga atatttgcat ttggcaccat ttgtttttgt tgcatctcca
    19201 tcagtttact ctgatcttta cctactatca atgatgtggc agtacctgaa attgccattg
    19261 taagaactac ccaaagtaga aactgcgtca atcccaccaa tgcaatacct ataatttttc
    19321 ccatcatcaa ctgaaatggt tttactgatg atatgataac ttcaacaatg cggctggttt
    19381 tttcttctat cacaccacgc atcacctgta tgccatacat gaaaataaac atataaatca
    19441 gcagtcctgc accaaagcca attccggcag aaacacctgc agaaaatgat ttgtcttttc
    19501 cactcgggtc aacgggtttt tgctgaagac tgatgtctgt ttttatagaa tcgagtgttg
    19561 atttttgaat acctgcatcg gtcagtttca tgttttcaat tttcttactc aactcacttt
    19621 ccaaatattc aactacagaa atgttaggtt gcttctctgt ataaagcact gttgcatcag
    19681 gcttctgcaa ggcgttggca ggtaagtaaa ggataccata atcagtattt gaattaaaca
    19741 aatgccttgc cgtttgaatg ggcatggtgt ctttagtaaa gattactgtt tttgaattat
    19801 tgagtttacc ggcaagcact cctgtttcgt ccaccacacg aatcattgat atggagtctc
    19861 cttgattcat ggaaaggtat gcaaatcctg caatgagtcc acccataagc aatggaccca
    19921 atatggtcat tattataaaa gattttttct ttacacgtgt cagatattca cgttgtataa
    19981 tcagcagtat gatgttcatg cttctacagg tttgttattt tgtaccgatt caataaaaat
    20041 atcattcata gtgggtatgc gctcacggaa agaaactact tccacttcgt tgagcagttg
    20101 tcgcaacaaa tcgtttgtac tgctgttgtt caggtttttt attactgcaa atgcatggtc
    20161 gtcttgtgca cgtgtttcaa ccaattcaaa gcctgtccat agtgcattgg caaaacctac
    20221 agatgtacct ttgtattcaa tctcaaaaag attagaacga aattgtttgc gcacatcttt
    20281 tacctgtcca tcaagaattt ttttcgattt attaatcagt gcaatgtgtg tacacagttc
    20341 ttctacactt tccatgcgat gtgttgacag aataatggta gcgcctttct gtcgcagccc
    20401 cagcaattca tctttaatca ggttggcatt gataggatca aaaccggtaa acggttcatc
    20461 aagaataagc agtttgggtt catgcagaat agtcacaata aactgaactt tttgctgcat
    20521 acctttgctt agctcttcaa cttttttgcg ccaccagctt tgcagttcaa atttttcaaa
    20581 ccaatagcgc aactttttta aagcatctgc acggctcaaa ccttttagtc gcgcaagata
    20641 aatagcctgc tcgcccactt ccattttttt atacaaccct ctttcttcgg gcagatagcc
    20701 aatttcagaa atgtgcgaag ggttcagtgt ttgtccgcca aataaaatac tgccgccatc
    20761 aggccctgtt atctgattga tgatgcgtat gagcgaggtt tttcctgcac cgtttggtcc
    20821 taaaagtcca aacacactcg actcgggaat ttcaatactc accgaatcta atgctttgtg
    20881 gtttgcataa atttttgaaa cctctcgtat agaaagcaga ttcatagttc aatattatgc
    20941 gacaaatgta atgtttaagt aatgtgataa aaatgaaatc caaacttggt tttaaacaac
    21001 actgcttatt gccacataaa aattatttct atctttccac ctttcagaaa ttgcacctat
    21061 gcgtttactt tcatttgttt tatgtttcat gttttgtttg aatgtctccg tttgggcaca
    21121 gcaggcagaa tcagattcat tgcaacattt gcttcgcaca caagctgacg atacttctaa
    21181 agtaaataca ttgaacgaac ttagctatgt ctattatcag actgctgttt atgacagcga
    21241 attgtattat gcacagcagg cattaaactt agcgcaaaag ttgaattgga aaagaggcat
    21301 ggctgtttca ctaaaaaata ttggcagtgc ctgcgatgat atgggcaaca acagcgatgc
    21361 attggtttac tacggacggg cattaaatat tttcagctct attgatgata agtccggtgt
    21421 ttctgcttgc tataacaata tgggtttggt gtacagaaca cttggcaatt tttcagaagc
    21481 gttaaagaac cattttgcat ctttaaaaat taaagaggcc attggcgata aaaacggcat
    21541 tgcactttca tacaacaaca ttggtgtagt gtttaatgct caaaaaaatt atgacgaagc
    21601 attaaaaaat catcgtgcag ccttagccat aagattgtct ttgggcaaca aaagaggtat
    21661 tgcctcatcc tatggaaaca taggcaatgt ctatcaggca caaggcaaca acaccgaagc
    21721 gctgaagaac tataccgaag cacttaaaat agcagaagaa accggtgata aagtaggtat
    21781 gacaacagcc ctcaacaata tgggtgcagc ctattttgaa atgggcgact atgaaaatgc
    21841 cttgcaacac aatactgatg ccctacgcat tgctgaagag gcaggcgacc tttcttcaat
    21901 tgctgctgca agcatcaaca ccggcaatgt catggtgcag atgaaacaac cacaggcagc
    21961 aactgaatgg cttcagaaag cattggagat tggtaaagaa attggcgaag ttgaagttat
    22021 aaaaaacagt tacgaaggac tcagtcaggc cgacagcgct ttgggaaaat acagcgatgc
    22081 actgcaacac tacaaaatgt ttatccgcta tcgcgacagc attgttaatg aagaaaacac
    22141 actcaaaacc gtacagttac aaatgcaata tgagtatgat aaaaaagatg ccattgcccg
    22201 tgctgttcag gaaaagaaag atgaagttgc acaacaggaa gtgcagaaac aaaaattact
    22261 cagaaacagt tttattgccg gatttgcatt gatgcttgcc cttgctgccg ttagctacag
    22321 aagctttgtg cgtaaacgaa aagacaacca tctcatcgaa aaacaaaagt tactcgtaga
    22381 agaaaaaaat aaagaaatta cgcaaagtct ccactatgca cgacacattc aggctgctgt
    22441 tttacctaat caaaaaaaca ttgcagcttt actgaaaaat cactttatac tttacttacc
    22501 taaagatatt gtaagcggag atttttacag tgtattgcaa aacaaagaca aggtaatagt
    22561 agctgctgcc gactgtaccg gacatggtgt ggcaggcgct tttatgagta tgattggtac
    22621 ttcgttaata aaccatctgg taaacgaaaa aaacattacc aaccctgcag ttattttaga
    22681 cgaactgaat gaaggcatca tcaactcttt gaaacaacgg cagggcgatt ctaacgatgg
    22741 catggacatt ggcttgtgca gcatagattt taaagaaaat aaattgcttt ttgcaggtgc
    22801 caaccgtccg ctttggcttt tcagaaatga agaaataaaa gtaattaaac ccgataaatt
    22861 tccgattgga ggcatgcagg ttgaccataa aaacaaatac actgcacaca gcatagattt
    22921 attgcctaac gacaccattt atattttttc tgatggctat tccgatcagt ttggtggcga
    22981 acagggtaaa aaaatgatga gtaaaaggtt taagcaatac ctgcaggtta tgcagcacct
    23041 gcctatggac aaacagaaag aacagcttga acagcttttt aattcatgga aaggcaatca
    23101 cgaacaggtt gacgatgtat tggttgtggg cataagagtg tgatgttgcg gcacttaaaa
    23161 aatgttaaaa actaaatttt tcaattaaag ccgttgtttt atttttgcaa cgttgccaat
    23221 agcaacaaag aacttatttt gtaaatcatt aaaaccaaat attatgttta aaaatttatt
    23281 cttttcgaag ggtgtaaaaa gtattgccct tgcagcattt tgtttgctga cttttaccct
    23341 aggtgccaat gcacagattt tagcatggga atttaatgga gcagcaggaa atgaaactac
    23401 cattgcagcc accacacttg atgctaattt gaacagttca tcagtttcaa gaggtagcgg
    23461 tgttaatgct tctggactta ctaatgcttt tgcttcaacc gattacaccg taagcggaac
    23521 ttttgctaat gcagttacaa acaacgaata tcagcagttt tcagttaatg caaaatctgg
    23581 ctacaaagtg tctctttcaa cattagatgt taatttcaga agatccaata caggtcctaa
    23641 cacattccaa tggcaatata gtttggatgg ttttgcaaca gcaggtatta atattggttc
    23701 tgcaattagt tatacaacca gtccaacaaa tggaactgcg caaagtcaaa tagatttaag
    23761 tgtaatttct gctttacaaa acgtaagtaa tgcaacaact attactatta gactttatgg
    23821 ttatggggca tctgcaggaa caggcacttt tgcgctagga aggctgacag gtaatgatct
    23881 tgcaattgga ggaactgttg atcctgatgg tggcagccct gtactcactg tcggcacact
    23941 ctctaatcaa ttcggcaatg tttgtattgg tactacaaca aactacgaaa catttactgt
    24001 ttccggtaca gacctcgatg gttcaagcgt agatgttggt acattaagcg gatttacata
    24061 ctgccttact cccggtggaa catatacttc aacactcaat ctgccctata cggcacctac
    24121 attaagcagc actactgttt atgttaaatt ttcgccaacg ctcgttcaaa gctatgcagg
    24181 ctctattcct gtaaccggtg gtggcgcatc tgccggcagt tgttctgttt catctgcatc
    24241 aggtattaat acttcacctt cactcaccac aggcagtgca acaggcatca cacaaactac
    24301 tgccacctgc gaaggaacta ttaccgataa cggatgtact agcgtttctg cctatggcat
    24361 tgaatatagc actacaaaca actttactcc cggaacaggc acacaggtta gcagcagcaa
    24421 catcagtggc aacacctaca cttctgcact aacaggtctt gcgggcaaca caacatacta
    24481 ctatcgtgcc tatgcaacca acactgcagg cacaggctat ggtgcacaag catcattcac
    24541 aacagatgcc tttactgcac ccgtagcaac agcagctacc aacattggca ttcattcttt
    24601 ccgtgccaac tggaacagtg tttccggtgc cacaggttat tttttagatg tttctacttc
    24661 ttcaacattt ttggctcctg ttacaactga tttggtgtat tgggattttc caaatgatcc
    24721 cgatgatgct actgccgatg gtggaatatt aagtaacctg tctgcaacaa tttcttctac
    24781 tgccggtggc actatgacgt tttctactaa cggagcttca acaaaaagtg ccagtataaa
    24841 caattgggat aatggtaacg gcaccaaata ttgggaagta agttttgatg caacaggata
    24901 ttataacctt gcattttcaa gcaaacaaca atcttcaaat accggtcctc gcgattttaa
    24961 agtgcaatac aaaattggca gtggtggcac atggacagat gtaacaggca gttcagttac
    25021 tgtagctaat aattttacta gcggtgtatt aacagatatt gccttacctg cagcatgcga
    25081 caaccaaagc agtgtgtatc tgcgttggat tatgacaagc aatacttctg ttggtggtgg
    25141 ttcggttgca gctaccggca ccaaccgtat tgatgattta tttgttaccg gctacgaagg
    25201 tgattttgtt ttagaaaacc aaagcgttgc aggtttatat tatgatgtaa caggtctgaa
    25261 tcctatcaca acttactact accgtgttcg tgctactgat ggtgtagcta cttcagacaa
    25321 cagcaatgtc attaacctta ctaccttaga tgtttgcgat ttgtttaccg taacagcatc
    25381 aagcaatgca ccaatctgtt caagcaccaa ccttaacctt aatgccaatg caagtggttc
    25441 cgatggaact atcaccggct atgagtggtc aggtcccggt ggctacagca acatgacaca
    25501 aaatccttct gtaagcaatc cgcttgcagg aacttataca gtaaccgtaa ctgatgataa
    25561 tggatgtact gtaacagaca atgtatctgc aaccgtagaa gcagcaccta ctgccaatgc
    25621 aggaagcgat gatattattt gctccagcgg cagtgcagca ttaaatggta taatcggtgg
    25681 cgatgctacc ggtggaacat ggacaactgc tggcgatgga actttctctc caaatgcaac
    25741 aacattaaat gctgcttatg ttcccggaac aacagacatc agcaatggca gtgtagtgct
    25801 tacactcact tcaacaggca gtgtgttgtg tagtgcagtt aacgatcagg taacaattac
    25861 cattcaaaca ggtgtgcctt cacaacctgc agttgtttca ggagcgccaa caagtgtttg
    25921 tcctcctgca ggtccttata ctttaacaac atcttcaact aatgccaata gctacaactg
    25981 gtatgttgtt ggtgccaaca caggaataac atttttatct gccaatggca gcaactcaat
    26041 ggatgtgcaa tacggtgtaa cagccaacag cagctactct gttcgtgttg aagcaagcaa
    26101 tgcctgcgga acaagcagct atcgttctgc atttacacgc agatcagttt caacacctgc
    26161 tactgttagt ggaaacaaag tagcttgtgc caccaccagc gatactta
//
LOCUS       DAOOOL010000018        25540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_149_length_25540_cov_60.494958, whole genome shotgun sequence.
ACCESSION   DAOOOL010000018 DAOOOL010000000
VERSION     DAOOOL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25540
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149_length_25540_cov_60.494958"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKU82_02790"
     CDS             complement(<1..184)
                     /locus_tag="PKU82_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOZ81830.1"
                     /translation="MSGLIMAAQLILGLSILVTLHELGHFLAARMFGIKVEKFYLFFD
                     AWGIKLFSFKKGDCEYG"
     gene            complement(200..1369)
                     /locus_tag="PKU82_02795"
     CDS             complement(200..1369)
                     /locus_tag="PKU82_02795"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015268361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOZ81831.1"
                     /translation="MKKRIAILGSTGSIGRQALEVIAAHADAFEVDLLTANGNIELLE
                     QQVRQFTPATAVVTDKEKYKTFKENIADLPVKVFSGIESAEQAVQSDSIDIVLTAMVG
                     YAGLLPTIAAIKSKKTIALANKETLVVAGKIITSLADDNGVRIYPVDSEHSAIFQCLA
                     GEFHNPIEKIYLTASGGPFRGKSKKELEQVTLAQALKHPNWSMGAKITVDSATMMNKG
                     LEMIEAKWLFGLQPEQIDVIVHPQSIIHSVVQFTDGSMKAQMGLPDMKLPILYALSFP
                     HRLKTDFKRFCFLDYPQLTFEKPDTEVFPALHLAEEAMKKDGNMPCILNAANEVAVAA
                     FLKEKISFFQIPGITEQLMSKITYIEKPSLEDLIATDKETRMQTELLLTKKINFA"
     gene            complement(1481..3316)
                     /gene="glmS"
                     /locus_tag="PKU82_02800"
     CDS             complement(1481..3316)
                     /gene="glmS"
                     /locus_tag="PKU82_02800"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOZ81832.1"
                     /translation="MCGIVAYIGYRQAYPIVIKGLQRLEYRGYDSAGVALLNGELSVY
                     KKAGKVADLANYVSDKNLVGQVGMGHTRWATHGEPNDRNAHPHYSESKDLAIIHNGII
                     ENYAAIKQELINSGHTFKSDTDTEVLIHLIEEIKHKTHVATEEAVRLALGEVIGAYAI
                     VVLSKSEPNKMIAARKGSPIVVGIGKDEFFIASDATPIVEYTKNVAYLNDGEVATIDN
                     GKLTVKTIDNETKIPFVQELELKLEAIEKGGYDHFMMKEIYEQPRSIWDSMRGRIDAD
                     KGRLRLGGISEFENKLINAKRIIIVACGTSWHAALVAEYLIEDLARIPVEVEYASEFR
                     YRNPIISEDDVVIAISQSGETADTLAAIEMAKERGATIFGVCNVVGSSIPRATHAGSY
                     THAGPEIGVASTKAFTAQVTVLTLMALQIANKRGTITQSRFRALLNELETIPSKVEIA
                     LKSNDKVKEISALFKDSRNFLYLGRGYGFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDEEMPVVVIATKNSSYEKVVSNIQEVKARKGKIIAIVTEGDVDVRKMADYTIE
                     IPETDDILVPLVSVIPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(3323..4615)
                     /locus_tag="PKU82_02805"
     CDS             complement(3323..4615)
                     /locus_tag="PKU82_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOZ81833.1"
                     /translation="MQHFKRLWMACVVLLSISCNKPDSVGLNLLPGEDAMNVEFFDTL
                     TLQTSYKQEDSLRTDAHLLYELQATNHQFMLGKYVDPIFGETSAELFTQLKPTAVFSN
                     TNKTYDSLFLNLAYISNYGDTSALQTFEVYELTDAMYDTASYYAFSNLNYSTKVGEIT
                     FKVEDAQDSIPTSDTTKIAPRLRLRLDDVLGQKIFNSDLSDPSKFKETVKGFCIKPAA
                     SSFGSIINFDAKTTVSSMIMYFTDPATTGIDSLPFYIYSSDGSIARFSRFTHDYSAFN
                     FTNPLTDKAYVQTMGGIKTKISIPYLNSLKEMGPISINKAELIINVDPGTATNLPAPD
                     ALSLYAIDSAGQIQTLPDLTRSSTLFGGGLANNQYRFNIAQFLQQILYEKRIDYGMYL
                     RVRVPVAFQTPNRVALVGGDPASTLRMKLQITYTKLNP"
     gene            complement(4665..5492)
                     /locus_tag="PKU82_02810"
     CDS             complement(4665..5492)
                     /locus_tag="PKU82_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOZ81834.1"
                     /translation="MIKKPKVLFVSQEIFPYLESSTIGNIARHLPQGTQEKGKEIRTF
                     MPRFGVVNERRNQLHEVIRLSGMNLIIDESDHPLIIKVASIQAARMQVYFIDNEEYFQ
                     RKHTTHDADNNFFKDNDDRTIFFCRGVLETVKKLGWAPDIIHCHGWMSSLIPLFVKTA
                     YREDPIFRNARVVYSVYDDEFSESFHDSYSKKLMMEGISTNDVKDIKSPNFESITIAA
                     INKSDAIIKGAEKLNTKIDNHIKKSGKLILPYQSPENYVEAYNTFYDEIMEEISIEA"
     gene            complement(5653..7029)
                     /locus_tag="PKU82_02815"
     CDS             complement(5653..7029)
                     /locus_tag="PKU82_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ81835.1"
                     /translation="MNQTMRLILLGLLVFASRWFFLDAGYGAEEDAWGYAVNALAMDS
                     TNSYQYSRLPGHPVPEWVYYFLPVKHSFAFNLCTAMLSTIAVLLFYLICKKYKLNAIA
                     GALMLAFTPVFFIQSTNAMDYNWSLCFMLAALFFAGKRNMILTSIMLGLAIGCRITAV
                     LFIIPISIYLLKADRSWKLVLQLLFLSGVVSALLYLPLILKYNLAFLTYVNQFGYPQI
                     LKTIYKASFGVWGTLGFVVIIIAFSYALQNIFNGMHSRKYKPILLCAASTLIIYTLLF
                     FYEPHKSAYLIPVIPFVFLIILIALQDKVWPVVITASLVISCFLGGINLADSHRSALP
                     GKYAIVRKAGSQLIAFDPLSGNVTDDHSKRIQRIKYAGSVVEQAMQLKGKNALICGYW
                     INMVSILGIGQLSDKQTLLHYADEQELIKLKNANTEIYYIPGQDSFNDVCFKNVFTRK
                     WAKSLFPQ"
     gene            7131..8117
                     /locus_tag="PKU82_02820"
     CDS             7131..8117
                     /locus_tag="PKU82_02820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HOZ81836.1"
                     /translation="MAMLLELTNKGIYCPQADVYIDPHRRVENAIITHAHSDHARPGS
                     SHYLAHKDSEAMLRMRLGEQISLRTVAYGESFTINGVKFSLHPAGHIIGSAQIRVAYK
                     DEVWVAAGDYKLHDDGFCPVFEHLKCNVFITESTFGLPIYKWQPQQQVMQEIDTWINK
                     NNQQQITSVLMGYSLGKMQRLIKHLNHFDFGIYGTPDVVAVNDRLREAGCNLPYVKPL
                     KSNPEKENLKGALIFASPSGARSALFKKLDNYSVGYCSGWMLHGKPKYLSHVDRGFAL
                     SDHADWDELNTAVKESGAEKIFVTHGSTASFSKWLCDNGYDAKEIELMKGAG"
     gene            complement(8135..10033)
                     /locus_tag="PKU82_02825"
     CDS             complement(8135..10033)
                     /locus_tag="PKU82_02825"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ81837.1"
                     /translation="MKKIKLIIILLLVSNMLQSQTIVLQETFDGQVMPTGWHEDSAGF
                     APVINQWNFIYYPFTMNGIGFDASFIRTGIANHDYSLVSPSFSTVGLSDVYLSYSDYH
                     QGPIDSGYQNVEMSVDSGQSWTIMLSNNGYNHYLGQNGSRRVVHLGTNAANQPNVKIR
                     FHFKVGGGWRWAIDSVVVNDYQLCTTPTDSTGVVYSSKDFLCSGIAGFFWIDHVSGGI
                     GQTYQWQYKPWGSSSYLNIAGAVYDTMTFVQNTPNYYRCLVVCNADTAGSMVTFVTDS
                     PTVVPKLRATNFSGICPGSPDTTYIFCEKNIPGIGYQWQWKQNIADPFTDIAGANDTL
                     YAIDAAVFPNQIYLRCKQQCIQSGKYLATATEVIFENPNPSCYCIPYNQNYCGPSTPI
                     FDGGIVDMHIAGTTLNNPSSTCSNIYGYPGQLSNYTYFDPTVANQTATLSRNVPYSMT
                     ITTDTLQIWFKGGLWIDYDHDSYFENTEYTTIDSLYPGTPRTITFSIPATATPGLTGM
                     RLRVAGNFPSVLNANSACTYAYGGETEDYMITIDTIVGIGEVKQHEANVQLYPVPASG
                     YIVVQSSQTIRSVRLESTTGALVKQDNGLNTNNWRMDVSSYAPGMYLMRTETSAGVVM
                     KKVMISRE"
     gene            10292..10921
                     /locus_tag="PKU82_02830"
     CDS             10292..10921
                     /locus_tag="PKU82_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81838.1"
                     /translation="MLKTGNTHFNIGILNVDSLFADAFLRLLNSFNDNLTIKHVSEAS
                     IASFYSFDILFVHQSRTWDYLNFLLSQKSNINAHETIYCLNCNTSLLLQKDVTDTLKI
                     LDLTIPVNDIRENLQHHVRKINKGKAKFGRPARSLTVLSRNFQLAGHFSDEELNIIYS
                     LRKHICNKQISVSCKISVSTLKRRLKAIADKCGVPNNKAAILCHLYERM"
     gene            complement(10934..12916)
                     /locus_tag="PKU82_02835"
     CDS             complement(10934..12916)
                     /locus_tag="PKU82_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81839.1"
                     /translation="MKRFLLSIIIFCTIINITVAQEQAGSIPVAVPVAQGVWVYLGNS
                     IPRNMHYQIERSKGDSKRFEKLGETTAPSSEQEMKNRQLPFSKYFEKLDPINDKTINR
                     LWQSTQKYPKADSLFSSNLPMMHLMAGTAYFDATAEKNENYIYRISLIASDGKSIFTK
                     ESNATAQFKKASLPKINFESCKYAEGKLVLTWSVKDPLRMSHFNVYRTIFGKEEYAKI
                     SIERGVYSENNNLMLLAIDTIGNKPAWYEYKIAAVDAYGNEGELQAIASGGSVEDYYA
                     PPITNLKAINTNSNHEVRLSWRLEKKKYLNGINVMRSTLFDSGYQRIATLPVSDTSFT
                     DIVPLSGENYYYYLQILSANDKPVSSAKVFAAYSGDKSVPLPPKEIDATSQPKGINVY
                     WKSDEPFAKGFYVYRRDNTTVPFVLISPLIASRQPIYSFSDTSQQLQAGGVYQYIVRT
                     ISDNNQLSKPSDTVSANPGIKKAMTPPTNLRFRENNGMLTLLWDDMRNWESNLMGYKV
                     YRKTSNGAFVKMDNDSLKSERNFFEDSELSSGTSYVYGVKTFDNSGSESEMATISFTA
                     VGELLAGPPSGISVSQTGDNVFITWGQISGDIVAIKIYKSEKGIQPKLIATITDVDSY
                     TDKNVFKGKLYFYQLSTVGKSNKEGAQSEKISIRVK"
     gene            complement(12978..18839)
                     /locus_tag="PKU82_02840"
     CDS             complement(12978..18839)
                     /locus_tag="PKU82_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOZ81840.1"
                     /translation="MSQNSFLTSINVILKPPYSSNYSLYENLSNHAIITLVGGARPMD
                     IVLYGTLVNTDRDLVIQTRSGYMGGAFNLGINQTKVIINDVPSMLFLNRNNVDPSGVP
                     DAVWSNILKTGQLPDGHYNFCVEAFEITSAAGLISAGSSCYEFNISLAQAPIIISPFD
                     GQNLNPQLPNTVFSWTPPIGNIMGANIVYDLYVVKIPQGSNPNDVMNAAVNYNANNPV
                     KKINLTGNQYVTQPYDLQIDTNTLYAVQVVARDLNKQVSFVNDGRSEVVTFTKGKTEI
                     PLVGQIKIEDPLKNPSQQSPGFNVTNLDPVPMSQLKGKLVYSFKDSYTSASVSNQPIL
                     GGPTANTNNPLLTAGGSSNPDDLVYNTDNTSLGNALPLAGKKVSLVITYLFTGTLNGK
                     NLNGQPGINNHGGFSPPDADKVLASTVTGADGSFKFDFLNVEKVLGLIDSNFTFKHTG
                     EFGDNVKGKIFKVLRIRVEDRYYCSPDINIKIDPWKGIDLGTVVSYVKSYTLKVKVLS
                     TSAKYWDMAYGQGSALPGITTSLLRKSNPPSSVPKNEGDGKGALSSIASAESDKDGYV
                     TFKHLVQHNPDDKSDYYSIKCTPSKTKGDFIFKERVDRYYPLYDKELLLFPFNATGSY
                     TPHIQGGINLPVAYGESIIWNHELEIKTYTDSMMLYPDNPRIAGKVQDAINIEGKTMS
                     NVKVVMINAYTQSVDPSKLFTTVLTDENGRYEFNDLPLEKGEFKVGEVVSIIGPTRTI
                     VVQPNGYKAAVLPAGAQQSDSLWNLGNKGKKIVPTCTSCYPPLKFGQQLLNQDFFLTP
                     DGFLSGYVVDENNNPVKADIDVDGYTKTSTKFQFTYGSSGNMTYRRVSNNKRGPLNAP
                     NNSGNNSITNIVVPTGTREYFSMAAPSGKRKVTVSPVDKAYALKDTTFVIKKEDNNPV
                     EIKFVVTRSQKRIRFKVAEFPLLSVSRLTVHEAENAKPIAGASVQLDIPGKPIIQMSD
                     KDGYVTFIFDNSASDFEFIITPPENANYETGNYKLTGVKNTTTITTYNNAYLKKAATI
                     TGTVTLGADKKALSGAKVYIETGNGKSIETTTGSDGKYVLKGVSTSPKEKTVWACKPG
                     ATPNIISQNKKITLSQQNELDFNLISDNELIIENIFGFEVDIQSKVKQSDGIWLVSGN
                     LINLPANDNFSLHDSKQKISFTDLKIKKSDATKNGIPVGVSVENTFSTDLTNIKLLLQ
                     NSFGVIQKPSSGDVLQVKSDNQKGRLTGKIAVQKSSFKFTQSYITFNNDPNEAMLITD
                     KPGSFITDISSIDIATSTKKKFGLANLQGKTMKFSLLGFDAEADAINSWLQDNSINLQ
                     TIIHINALPDMTPSTLDINAGNLIIHPEKLEPLKGNQPIKFNLEKWQFIGNNWQLSQG
                     DNSINIATGTIKTGSIDIPISDISLQPDHIGIGDFDVNNLSFGGIIPVHVTSTNPVFG
                     RNKSIGSDQKPHYEFRLIGENGSPGVVIKTLPGMKTGEEMKFQNLSLISNGEQIINPG
                     NQPNSITFYSVMKVKPLSFMSGQDYVNMTCGIDLGIPQLKETSGIIQFSKEAGQIKFQ
                     LYPLNVSLKGPGDVDFVANVQFNDRPQNLTEGKFTALGTIYEKEGMVLKAILNKTTTA
                     AWIQVDPENQKLPLGGSTTSLANIKGKMEADMSSGIWKNFTFSGEMQGFKGMQGDTRK
                     TFVVTGSINASNQNIEVKNIPSGFGNIGLNYDFVNSRFTGNLQLDKNIGPLALAGTAN
                     LLVDPDGWYFLAGGKVQAPGIGAMSAGLLIGDYSKMPGDVSGALMQFAYDKHVPPSFQ
                     NGISGFFFTGMKELPVLNIPNYSIDLGVISASFGAQAGLDARLWMDFASAGNEYGIGA
                     MVFAHAYLKGASITCTKFGADARAELGMKGSYATSTGTFSLNGCGSFTISGSIKQCFP
                     TPCFSDGICCEGCGGIGVSQGIKVNLLLDSNGATDLSFGFGNCSGQQVMGGSW"
     gene            complement(18936..20660)
                     /locus_tag="PKU82_02845"
     CDS             complement(18936..20660)
                     /locus_tag="PKU82_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81841.1"
                     /translation="MQKKSQNEKKSYEISLSLFLLFLFFTIISKAQDVEHINLKKPVT
                     FHGNLNFQLDYYSANGIPARQKEFSWIINGNPVLNVLGIDLPFTFLFSNFGNKYSQPF
                     NQFGLSPRYKWITMHLGYRNLTYSNYTLAGHKMLGVGFDLNPKKFRIGFMYGQLRRSA
                     SIDSTMNTNPMYVRSTPTFKRVGIAGKIGYGSQKNYVDLVYFKGWDKPSSLNGKLKDS
                     IQPAENTSFGLVSTLSLSKKITWTSDIGFSAYTLNRMDRKDSSDIDKAWPKDIMGPLV
                     SDKLSTQYYFAGETKIGYQEKKWGAQLKLKRIDPGYQSMGAYFFQNDIKEYSLANNFK
                     LDSNRLNINTNIGIQTDNLKKQKSSTSKRFIGSVNVNYVPSQKFGISFNYSNFGITNS
                     PLQTSPGNEQYKQVNNSVMLMPYLLWTNEKTFKNLNCIATFQSLSTPQSYGNNTPDMN
                     TFSFSTNYSQTWIKPGINANVAMNFINSKTTAGDVGSIGGSVGGSAPLMKKRLTLNTS
                     FSYLQNKFKGNDNGNTIRFTLGFMVPVGTHHNFQLMCNYLDNTSKDVSLIQNFKETNI
                     QFIYGLTF"
     gene            complement(20930..21331)
                     /locus_tag="PKU82_02850"
     CDS             complement(20930..21331)
                     /locus_tag="PKU82_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81842.1"
                     /translation="MLKDIPQLKVENVAVAIVKEQSNGADVWNAYIINLKNEMLEGVL
                     ITSKGYGMMEGEERKTSTLRHFIENIPPQSCVKIEPITDNVLNLNNEYWVSFYLNNTM
                     YDRKYVFLPGVINEEFFTQIPLLEKRGVMIK"
     gene            complement(21378..22511)
                     /locus_tag="PKU82_02855"
     CDS             complement(21378..22511)
                     /locus_tag="PKU82_02855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ81843.1"
                     /translation="MKKIIKYLLFILLITTVVLAATGKLYYYKALVYNFVDIDDLNLF
                     HNREVKNGTGMPWNVSTDYNKKNLSDTLTALLEANKSVAFLVIKNDSICFEKYWEDYG
                     QQSLSNSFSMAKSIVALLIGKAIDEGKIKSLDQPVADFIPEFKEGEKSKITIRNLMMM
                     SSGLDWNEGYSSLTSQVTEAYYGTQLYKQVASLGVAEAPGKFWEYKSCDTELLAIIVT
                     NATGKSLAEYASEKLWKPLGAEHSAQWSLDHKDGIEKAYCCFYSNARDFARFGKLVLQ
                     HGRWNNEQIIDSAFITLCTTPNNLPDKEDNKPVQHYGYQWWLSNISNHPVYYMRGILG
                     QYVIVIPDMQMIIVRLGHKRSNNPDGKPNDYKFYAEQVIKMYE"
     gene            complement(22508..23245)
                     /gene="lipB"
                     /locus_tag="PKU82_02860"
     CDS             complement(22508..23245)
                     /gene="lipB"
                     /locus_tag="PKU82_02860"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOZ81844.1"
                     /translation="MQEILFADLGASDYKQTWDFQEQLLQQIMQLKLEGRNHGFGDHA
                     PTPHYLLFCEHPHVFTLGKSGKPEHLLSNEQQLKTANATYYKINRGGDITYHGPGQIV
                     GYPILNLDLFFNDIHRYMRFLEEVIIRTLKEYDIEGSRIEKLTGVWIDAGKPSARKIC
                     AFGVRCSRWITMHGWALNVNTQLDYFNHIVPCGISDKAVTSMAKELGKNLNIDEVKSV
                     LLKHFSEVFNATIKNVGKEKLIAKEKT"
     gene            complement(23311..24567)
                     /locus_tag="PKU82_02865"
     CDS             complement(23311..24567)
                     /locus_tag="PKU82_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOZ81845.1"
                     /translation="MLRIPSLASLIFICAFLQTHFVFAQQDDARKVITELTSEKYFGR
                     GYLKHGDKKAAHFIKKQFSKLGLKPVDDKHWFQHFTHPVNRFPKNVSLTTDTKNWIPG
                     KEFLIHHQSPSANGTWPIAWADSAEIISRLNNDYDSCYVITRQVFSKQQKEITALLFK
                     RKKGALVILEPHKLTWSVGTSVFNIPVVEVYDSLFSRQTKNITIKADAVFDAGYDSKN
                     VIGLITGNKTPDSLIVITAHYDHLGGMGNKTFFPGANDNASGVAMLLSLAEYFSKKEN
                     QQPYSILFIAFAGEEAGLQGSKYYTEHPILPLSKIRFLINLDLLGTGDDGIMVVNATE
                     FEKDFSLLQSINDIAKFVSNIGKRGKAKNSDHYYFSEKGVPAFFIYTLGGVTWYHDVM
                     DKAETLPLTKYNNVFQLLKSFVLALQ"
     gene            complement(24567..24848)
                     /locus_tag="PKU82_02870"
     CDS             complement(24567..24848)
                     /locus_tag="PKU82_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81846.1"
                     /translation="MAQPAFAIADKLKLSPVTDLVKAIGINEKFLFISQLFNDDSIKY
                     QSAIDKLNTSSNFAEAYMYFDSEITSGNQIDKNSDACRQFVDLLQRRFM"
     gene            complement(24824..25363)
                     /locus_tag="PKU82_02875"
     CDS             complement(24824..25363)
                     /locus_tag="PKU82_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81847.1"
                     /translation="MTKQSITSEIQVAIEQIYRQWGVFSLDNRIPENDELDMLKSKLM
                     KLHDKINHLQLLESVYAYKQPIQEVITETVQKPITQTVIEKSETEQKITIEAPEVKTF
                     EPIIAEVTEPTPIKPEVHTATTTAEEVKQKKVTASVVPPVQTARAEVFKEADQTVATM
                     FHETETIHDKLVWRNRLLP"
BASE COUNT         7651 a         4841 c         4486 g         8562 t
ORIGIN      
        1 taccatactc acagtcgcct tttttaaagc taaacaattt tattccccat gcatcaaaaa
       61 acaggtagaa tttttcgacc ttgataccaa acattcttgc ggcaagaaaa tgtcctagct
      121 catgcagggt taccaatatt gagaggccca gtatcaactg agctgccata atcaatccac
      181 tcatcagatt ttaaaaattt tatgcgaagt taattttttt tgtcaatagt aattctgttt
      241 gcattcgtgt ttccttatcg gttgcaatca ggtcttcaag tgatggtttt tcaatgtaag
      301 taattttcga catgagttgt tctgtaatac cgggaatctg aaagaaactt attttctctt
      361 tgagaaacgc agcaacggca acttcgtttg ctgcattgag aatacaaggc atgtttccat
      421 cttttttcat agcttcttca gccaaatgta aggcaggaaa aacttctgta tccggttttt
      481 caaaagttag ttgaggatag tcaagaaaac agaatctctt gaaatcggtc tttaaacgat
      541 gcggaaaact caatgcataa agtatgggca gtttcatatc aggcaaaccc atttgtgctt
      601 tcatactgcc atcagtaaat tgcactacag aatgaatgat agattgcgga tgcacaatta
      661 catcaatctg ctccggttgt aaaccaaaca accatttggc ttcaatcatc tcaagacctt
      721 tattcatcat ggtggcagag tcaacagtaa tttttgcacc catgctccag tttggatgtt
      781 tcaatgcctg agccagagtt acctgctcta attctttttt gctttttcct ctgaagggtc
      841 cacccgatgc tgtcaaataa attttttcta tagggttgtg gaactctcct gccagacatt
      901 gaaaaatggc tgaatgttcg gagtcaacag ggtaaatgcg cacaccgtta tcatctgcaa
      961 gtgatgttat aattttccct gccaccacaa gggtttcttt attggccagt gcaatggtct
     1021 ttttactttt tatggctgca attgttggca gtagtccggc ataacctacc attgctgtaa
     1081 gaacaatgtc aatggagtcg gactgtacag cttgctcagc agattctatt cccgaaaaaa
     1141 ctttaaccgg aaggtcagca atattttctt taaacgtttt gtatttttct ttatcggtta
     1201 caacagcagt agccggagta aattgccgaa cctgttgttc gagcagttct atattgccgt
     1261 ttgcagttaa tagatcaact tcaaaagcat cggcatgtgc ggcaatcact tcaagtgcct
     1321 gcctgcctat actaccggtc gaaccaagaa tagcaattcg ttttttcaaa gctgattgac
     1381 tatgagttga caatattagt taaatcctgt tgaagtctgt attaatcaac agggatattg
     1441 taaacagaaa tatgatgaga gaagtagttt aaatgcaacg ttattcaaca gtaacactct
     1501 ttgccaggtt acgaggctga tcaacattac aaccacgcat aacagcaatg tgataactga
     1561 gcaattgcaa tggaattaca ctaaccaatg gtacaagaat gtcgtctgtt tccggaattt
     1621 caatagtata atctgccatc ttccttacat caacatcgcc ttctgtaaca attgcaatta
     1681 tttttccttt acgtgccttt acttcctgaa tgtttgaaac cactttttcg tatgaagaat
     1741 tttttgtggc aataactaca acaggcattt cttcgtcaat caaagcaata ggcccgtgtt
     1801 tcatttctgc agcaggataa ccttctgcat gtatgtagga tatctcttta agctttaatg
     1861 caccttctaa tgcaacagga aatccataac cacgacctag gtaaaggaag tttctggagt
     1921 ctttaaacaa tgcagatatt tctttaacct tatcgtttga tttaagtgca atttcaactt
     1981 ttgaaggaat ggtttcaagt tcgttaagca atgcacgaaa acgtgattgt gtgatggtac
     2041 ctcttttgtt tgcaatctga agtgccatta atgttagtac tgttacctgt gcagtaaatg
     2101 ctttggtaga agccacacca atttcgggtc cggcatgtgt gtaggaaccg gcatgtgttg
     2161 cgcgtggaat agaagacccc acaacattac aaaccccaaa aattgttgca ccacgttctt
     2221 tagccatttc aatggcagca agtgtatcgg cagtttcgcc actttgcgaa atagctatca
     2281 ctacatcatc ttcagagatg attggatttc tatagcggaa ttcagaagca tattcaacct
     2341 ccacaggaat tcgtgccagg tcttcaataa gatattctgc taccaatgct gcatgccaag
     2401 atgttccgca ggcaacaatg attattcttt ttgcattgat caatttgttt tcaaactcgc
     2461 tgataccacc taatctcaac ctgcctttgt cagcatcaat gcgtccgcgc atgctgtccc
     2521 aaatagaacg aggttgctca tagatttctt tcatcatgaa atgatcgtag ccaccttttt
     2581 caatagcttc aagctttaac tccaattcct gaacaaaagg aattttggtt tcgttgtcaa
     2641 ttgtttttac ggtaagttta ccattatcaa ttgttgcaac ttcgccatcg ttgaggtagg
     2701 caacattttt agtatattct acaataggtg ttgcatcaga tgcaataaaa aattcgtctt
     2761 taccaatacc cacaacaata gggctaccct tgcgggctgc aatcatttta ttaggctcac
     2821 tctttgataa aactacaatg gcataagctc caataacttc gcctaaagca agacgtacag
     2881 cctcctcagt agctacgtgt gttttatgtt ttatttcttc aataagatgt atcagcactt
     2941 cagtgtctgt atcactttta aaagtatgac ccgaatttat cagctcttgt tttattgctg
     3001 catagttttc gataatacca ttgtggatga tggctaaatc tttcgattcg gaataatgcg
     3061 gatgcgcatt tctgtcgttg ggttcgccat gtgttgccca acgtgtatgt cccataccta
     3121 cctgcccaac caggttctta tcagaaacat agttggccaa gtcggcaacc ttacctgctt
     3181 ttttgtaaac actcaattca ccattcaaca atgccacacc ggcactgtca tagccgcgat
     3241 attccagccg ctgaagtcct ttaattacta ttggataagc ctgacgatat cctatatagg
     3301 caacgattcc acacatgata ttttatggat ttaattttgt ataagtaatt tgtaatttca
     3361 ttcgtaatgt tgaagcaggg tcgcctccca ctaaagccac acgattaggt gtttgaaagg
     3421 ctaccggcac ccttactctt aagtacattc cgtaatctat acgtttttcg tataaaatct
     3481 gttgcaaaaa ctgtgcaata ttaaaacggt actgattatt tgccagtcca ccgccaaaaa
     3541 gtgtagatga acgtgtcaaa tcaggcaaag tttgtatttg tcctgcggaa tctatggcat
     3601 aaagactcaa tgcatcagga gcaggaaggt tggttgccgt tcccggatca acattgatga
     3661 ttaattctgc tttattaatt gatattggcc ccatttcttt cagactgtta agataaggaa
     3721 tggaaatttt tgttttaata ccacccattg tctgaacata agctttatct gtcaatggat
     3781 tggtaaaatt gaatgctgag taatcgtgtg taaaccggct gaaacgggct atgctgccat
     3841 ctgagctgta aatataaaac ggtagtgagt caatgcctgt tgttgccgga tctgtaaaat
     3901 acattatcat tgacgaaacg gttgtttttg catcaaaatt gataatactt ccgaatgatg
     3961 acgctgcagg tttaatacaa aaacctttaa ccgtttcttt aaattttgat gggtcgctta
     4021 aatcggaatt aaatattttc tgacctaaaa cgtcatcaag gcgcaaacgc agtcgagggg
     4081 caatttttgt tgtgtcagaa gtaggaatgc tgtcttgagc atcttcaacc ttaaatgtta
     4141 tttcgccaac ttttgtactg taattgagat tactgaaagc ataataactt gccgtgtcat
     4201 acatagcatc agtcagttca taaacttcaa aagtctgcaa ggcggaggtg tcgccatagt
     4261 ttgaaatata tgccagattc agaaataatg aatcgtatgt tttgttggta ttggaaaaaa
     4321 ctgctgttgg cttaagttgc gtaaaaagtt cagcactagt ttcaccaaaa atagggtcaa
     4381 catatttgcc gagcataaac tgatggtttg tagcctgaag ttcataaagc agatgtgcat
     4441 cggtgcgcag gctgtcttcc tgcttgtacg atgtctgaag tgtcaatgtg tcgaaaaatt
     4501 caacattcat ggcatcttct ccgggcaaga ggttaagacc aacagagtcg ggcttgttgc
     4561 atgagatact tagaagcacg acacatgcca tccacaaacg cttaaagtgt tgcatataaa
     4621 taaggcgtgg ttttttaaaa taattaatgt tgaacaatat ccgcttaggc ttctattgag
     4681 atttcttcca tgatttcatc atagaaagta ttgtatgcct caacgtagtt ttcaggcgat
     4741 tggtatggta aaatcaattt gcctgatttt ttaatatgat tatcaatctt ggtattgagc
     4801 ttttcagcac ctttaatgat agcatctgac ttgtttatgg cagcaattgt tatgctttcg
     4861 aagtttggac tttttatgtc ttttacatca ttagtagaaa tcccttccat catgagtttt
     4921 ttagagtaac tgtcatgaaa tgattcactg aattcatcgt catatacaga atacaccact
     4981 cttgcatttc ggaaaatagg gtcttctcta taggctgttt ttacaaacag cggaatcaga
     5041 ctcgacatcc agccgtggca atgaataata tccggtgccc aaccgagttt ttttaccgtt
     5101 tcaagaacac cacgacagaa gaaaatggta cggtcgtcat tgtccttaaa aaagttgttg
     5161 tctgcatcat gagtagtatg ttttctttga aaatactcct cattatcaat aaaataaacc
     5221 tgcatgcggg ctgcctgaat actggcaacc tttataatca acggatgatc gctttcatca
     5281 ataatcaggt tcataccgga aagtcggatt acctcatgta attgatttct acgctcatta
     5341 acaacgccaa agcgtggcat aaaggttctg atttctttac ctttctcctg tgtgccttgt
     5401 ggcagatgtc ttgcaatgtt acctattgtt gaactttcga gataagggaa aatttcctgc
     5461 gatacgaaca ggaccttggg ctttttaatc atgcaatcta cttctcttaa aatttacgta
     5521 tgcaaaaata tataaaaatt ccctttgaac cggcaaaatt ttagcgatat tttgttaaag
     5581 tagctgatat gcaggcaaaa ggcttaatct ttaatactaa aaaaaagtag aaattgggca
     5641 attggggttg atttattgcg gaaataggct ttttgcccat ttacgtgtaa agacattctt
     5701 aaaacagaca tcattaaaac tgtcttgccc gggtatgtaa taaatctccg tattggcatt
     5761 tttcaatttt atcaattcct gttcatcggc ataatgcagc aaagtctgtt tgtcagaaag
     5821 ttgacctata cccagaatag aaaccatatt tatccagtat ccacaaatca aagcattttt
     5881 accttttaac tgcattgcct gttctacaac acttcctgca tattttatcc gctgaattct
     5941 ttttgaatga tcgtcagtaa cattaccact taatgggtcg aaagcaatga gttgggaacc
     6001 tgcttttctg actatggcat atttacccgg cagtgccgac ctgtgactgt ctgcaagatt
     6061 aatacctccc aaaaaacaac ttattaccaa tgatgctgta attacaacag gccaaacttt
     6121 atcctgcaat gcaatcagaa ttattaaaaa cacaaatgga attacaggaa taagataagc
     6181 agatttatgt ggttcataga aaaaaagtaa ggtataaatt ataagtgttg aagcagcgca
     6241 caacagtatg ggtttatatt tccggctatg cattccatta aaaatattct gaagcgcata
     6301 actaaaagct attataataa ctacaaaacc caatgttccc caaacaccaa aacttgcttt
     6361 ataaatagtt tttaaaattt gcggatagcc aaattgattt acataagtca gaaaagccag
     6421 gttgtatttt aatatcaatg gaagatataa aagtgcactc actactcctg ataaaaacag
     6481 aagctgcagc acaagtttcc atgaacgatc ggcttttaga agataaatac ttatggggat
     6541 aataaacagc actgctgtaa tgcggcaacc tattgccaga ccaagcataa tggaggttaa
     6601 aatcatattt cgttttccgg caaaaaacaa ggctgccagc ataaagcaca gcgaccagtt
     6661 atagtccatg gcattggtac tttgaataaa aaaaacagga gtaaaagcaa gcattaatgc
     6721 tccggctata gcattgagtt tatatttttt gcagattaaa taaaacagca aaacagctat
     6781 cgtactcagc atggctgtac ataaattaaa agcaaaagaa tgttttacag gcaggaagta
     6841 atacacccat tcgggtacag ggtgtccggg caaacgcgaa tactgataac tatttgttga
     6901 atccattgca agagcattta cggcatagcc ccatgcatct tcttcggcac cataacctgc
     6961 atcgagaaaa aaccagcggc tggcaaaaac caataatccc aaaaggataa gccgcatagt
     7021 ttgattcatg gacgcaaagt aacatgtttt gaccattttt atatgatgtt gattcaattt
     7081 taacaacaca tttatatctt tacgtaatta caaataattg cagcaccgtc atggctatgt
     7141 tattagagtt gacaaacaag ggtatttact gtccgcaggc agatgtctat attgacccac
     7201 atcgcagagt agaaaatgcc atcatcacac atgcacatag cgaccatgct cgccctggaa
     7261 gcagtcacta tcttgctcat aaagattcgg aagccatgct gcgtatgcga ttgggcgaac
     7321 aaattagttt acgcacggtt gcttatggag agtcatttac aattaacgga gtaaaatttt
     7381 ctttgcatcc tgccggtcat attattggca gtgcacagat aagagtagca tataaagatg
     7441 aagtatgggt ggcagcaggc gattacaaac tgcacgatga tggtttctgc cctgtgtttg
     7501 aacacctaaa atgtaatgtg tttattacag agtcaacctt tggtttacct atttacaaat
     7561 ggcagccaca acaacaggta atgcaggaaa ttgatacttg gataaataag aataatcagc
     7621 aacaaataac ttctgtactg atgggttatt cgcttggcaa aatgcaaagg ctgataaagc
     7681 acttaaacca ttttgatttt ggcatttatg gaacaccgga tgttgttgct gttaacgaca
     7741 gattgcgcga agcaggttgc aatttgcctt atgttaaacc gctgaaatca aaccctgaga
     7801 aagaaaatct gaaaggggca ttaatatttg catcaccatc cggagcacgt tcggcattgt
     7861 ttaagaaact cgacaactat agtgttggct attgcagtgg atggatgttg catggcaaac
     7921 cgaaatactt gagccatgtt gaccgtggtt ttgcactaag cgaccatgcc gactgggatg
     7981 agttgaatac agcagttaaa gaaagtggtg cggagaaaat ttttgtaaca catggaagta
     8041 cggcatcatt ttcaaaatgg ctttgcgaca atggatatga tgcaaaagaa attgaactga
     8101 tgaagggggc ggggtaagaa gaataaaact gtttttactc cctgcttatc attacttttt
     8161 tcatcactac accggcagaa gtttctgtgc gcatcaggta catacccggt gcatagctgc
     8221 taacatccat tcgccagttg tttgtgttta atccgttatc ctgttttact aatgctcccg
     8281 tagtgctctc taacctcaca cttcttattg tttgtgatga ttgcacaact atataaccgc
     8341 ttgcaggaac aggatatagc tgtacattag cctcatgctg ctttacttcg ccaatgccca
     8401 ctatggtgtc tatggttatc atataatctt ctgtttcgcc tccataggca taagtgcagg
     8461 cgctgttagc gttcagtact gatgggaaat tccctgctac acgcaggcgc attcctgtta
     8521 ggccgggtgt ggctgtggcc ggaatactaa aggtaatcgt ccttggtgtg ccgggatata
     8581 atgagtctat ggtagtatat tctgtgttct caaaataact gtcgtggtcg tagtctatcc
     8641 acagtccgcc tttaaaccat atttgtaagg tatctgtagt aatagtcatg ctatagggta
     8701 cattgcgcga cagtgttgct gtttgatttg caaccgttgg gtcaaagtag gtgtagttgc
     8761 tcaactgacc gggatatcca taaatgtttg agcatgttga agacggattg tttaacgttg
     8821 ttcctgctat gtgcatatca acaataccgc catcaaaaat tggggtagaa ggcccgcagt
     8881 agttttgatt gtagggaata cagtagcagc ttggattggg attctcaaat attacctctg
     8941 ttgctgtagc aaggtatttg ccgctttgaa tacattgctg tttgcagcgt aaatatatct
     9001 gattaggaaa cactgctgca tctatggcat acaaagtatc gttagctcct gcaatatctg
     9061 taaaggggtc tgcaatgttt tgtttccatt gccattgata gcctatgcct ggaatatttt
     9121 tttcgcaaaa gatataagtt gtatcggggc tgccgggaca aataccacta aaatttgttg
     9181 cccttaactt aggtaccact gtgggcgagt ctgtaacaaa ggttaccata gagcctgctg
     9241 tatcggcatt gcagactacc agacagcggt aatagtttgg cgtattttga acaaacgtca
     9301 tggtgtcgta aacggcaccg gcaatgttta aatagctgct gctgccccag ggtttgtact
     9361 gccattgata ggtttgacct atgccgccac taacatggtc tatccaaaaa aaacctgcta
     9421 tgcccgagca taaaaaatct ttgctgctat ataccactcc tgtgctgtct gttggtgtag
     9481 tgcataactg atagtcgttt actactacac tgtctattgc ccaacgccat ccaccaccca
     9541 ccttaaaatg aaaacgaatt tttacattgg gctgattggc tgcattggtt cctaaatgaa
     9601 ctactcttct tgaaccattt tgtcctaaat aatgattata tccattgtta gataacataa
     9661 ttgtccaact ttggccactg tcaacagaca tttccacatt ctgataacca ctatcaattg
     9721 gaccttgatg gtaatcacta taagaaagat agacatcaga taaacctact gttgaaaacg
     9781 aaggagatac taagctgtaa tcatgattag caatacctgt tctgataaat gaagcatcga
     9841 aaccaatacc attcattgta aacgggtaat aaataaaatt ccattgatta attacaggag
     9901 caaagcctgc actgtcttca tgccagcccg ttggcattac ttggccatca aaagtttctt
     9961 gcaatactat ggtttgcgat tgcaacatgt tgcttactaa cagcaatatt attatgagtt
    10021 taatcttttt cattttcttg tttttattgt gtgatgaaga tttttttaaa agatttattg
    10081 cttcaatatt aaagtcacat tacattttta aataaaaata aatcaaccat gaacgctaac
    10141 caactttatt gtttttcaaa attataaaaa gcctgaatag ctccaatgtt cataaccccg
    10201 aacttttggc tcaatttata tgcttaccgc tttttttctt gtcagttatc aaaaaaaatc
    10261 atatacttgc tttacttaaa caaccattat tatgcttaaa accggaaata cgcatttcaa
    10321 tatcggcatc ctcaatgttg atagtttatt tgcagatgct tttttaagat tgcttaattc
    10381 tttcaatgac aatttaacca ttaaacacgt cagcgaagca agcattgcat cgttttattc
    10441 tttcgatatt ttatttgtac atcaatccag aacatgggat tatctgaatt ttttgctcag
    10501 tcagaaatct aatattaatg ctcatgaaac aatatattgt ctgaactgta atacaagtct
    10561 gttattgcaa aaggatgtga cagacacctt aaaaattctt gacctcacca ttcctgttaa
    10621 tgatattaga gaaaatcttc aacatcacgt caggaaaata aataagggta aagcaaaatt
    10681 cggcagaccc gccagatcgc ttactgtact gagccgcaat tttcaattag cagggcattt
    10741 ttctgatgaa gaattgaata ttatttacag tttaagaaag catatctgta acaaacaaat
    10801 ttcagtgagt tgtaaaatat ctgtttctac tttaaagaga aggcttaaag ctatagcaga
    10861 caaatgtggt gtgccaaata ataaagcagc aattctttgc catctctacg aaagaatgtg
    10921 attgaagagt tgattattta accctgatag aaattttttc actctgtgca ccttctttat
    10981 ttgatttgcc aacggtggat aattgataaa aatacagttt ccctttaaac acatttttat
    11041 ctgtataaga atctacatcg gtaatagttg caattaattt gggctgtatg cccttttcag
    11101 atttataaat tttaattgct acaatatctc cggaaatctg tccccatgtg ataaacacat
    11161 tgtcaccggt ctggcttaca gaaattcctg aaggtggacc tgctaacaat tcacccacag
    11221 cagtaaaact aattgttgcc atctcacttt cacttcctga attgtcaaat gttttaacac
    11281 catatacata agatgttcct gaagataatt ctgaatcttc aaaaaagtta cgttcggatt
    11341 tcaaactgtc attatccatt tttacaaaag caccattact ggtttttcga tacactttat
    11401 agcccattaa attactttcc caattgcgca tgtcgtccca cagtaatgtt aacatgccat
    11461 tattttctct aaagcgcaga ttagttggtg gagtcatcgc tttttttatt ccgggattag
    11521 cacttactgt atctgaaggt ttgctcagtt gattgttatc actgatggtt cgaacaatat
    11581 attgataaac accacctgct tgaagttgct gtgatgtatc tgaaaaagag taaataggtt
    11641 gccttgaagc aattaatgga cttattaata caaacggaac ggttgtgtta tctcttctgt
    11701 acacataaaa tcctttggca aatggttcat cacttttcca ataaacattt atccctttgg
    11761 gttgtgatgt agcatcaatt tctttgggcg gcagaggtac tgatttatct ccggaataag
    11821 ctgcaaacac ttttgcagaa gaaacaggtt tgtcattagc acttagaatc tgtagatagt
    11881 aatagtagtt ttcacctgat aatggaacta tatctgtaaa agaagtatca ctcaccggca
    11941 atgttgcaat tctttgatat cctgaatcga acagtgtgct gcgcattaca tttattccat
    12001 tcagatattt ctttttttca agtcgccagg aaagtcttac ctcatgatta ctgttggtat
    12061 taattgcttt tagatttgtt attggtggtg cataataatc ttctacacta cctccacttg
    12121 caatggcttg cagttcgcct tcattaccat aagcatcaac agcagcaatt ttatattcat
    12181 accatgcagg cttatttcca atagtgtcaa tagcaagtaa catcaggttg ttgttttcag
    12241 aataaacacc tcgctctatt gaaatctttg catattcttc tttaccaaat attgttctat
    12301 aaacattgaa gtgtgacatt ctcaaagggt ctttaacact ccatgttaaa acaagttttc
    12361 cttctgcata tttgcagctt tcgaaattta tttttggcag agatgctttt ttaaattgag
    12421 cagttgcatt actttctttt gtaaaaatag atttgccatc agatgcaatt aaactaatac
    12481 gataaatata attctcattt ttttctgctg ttgcatcaaa gtatgcagtt cctgccatta
    12541 aatgcatcat tggaagattg ctgctaaaca agctgtctgc ttttggatat ttttgtgttg
    12601 attgccaaag gcgattgatt gttttatcat ttataggatc taatttttca aagtatttac
    12661 taaaggggag ttgacggttt ttcatttcct gttctgatga aggtgctgtt gtttcaccaa
    12721 gtttttcaaa tcttttacta tcgcctttgc tacgctcaat ctgatagtgc atgtttcgtg
    12781 ggatactatt tcctaaataa acccacaccc cttgtgctac aggaacagca acaggtattg
    12841 acccggcttg ctcttgtgca acagtaatat taataatagt acaaaatatg ataattgaaa
    12901 gaagaaaccg tttcattttt taaagatttt gttttattca ccatttaaat aatctattct
    12961 aaagtacaag attggattta ccagctgcca cccatcacct gttgtccact gcaattccca
    13021 aacccaaagc tcaagtcagt agcaccattg gagtctaaca ataaattaac ttttataccc
    13081 tgacttacac cgattccacc acaaccttcg caacaaattc catcactaaa gcaaggagta
    13141 ggaaagcatt gttttatact tccgcttatg gtaaaactcc cacaaccatt caaagaaaat
    13201 gtaccggtag atgttgcata acttcctttc attcctaatt ctgctctggc atctgcgcca
    13261 aacttggtac atgtaattga agcacctttt aaatatgcat gtgcaaacac cattgcacca
    13321 attccatatt catttcctgc cgaagcaaag tccatccata atcgtgcgtc taatccggct
    13381 tgtgcgccaa acgaagcact gataacacca aggtctatac tatagtttgg aatgtttagc
    13441 accggcaatt ctttcatacc tgtaaagaaa aatcctgaaa tgccgttttg aaaacttggt
    13501 ggcacatgtt tatcgtatgc aaattgcatg agtgcaccgg aaacatcacc gggcatttta
    13561 ctataatctc caataagcaa tccggcagac atggcaccaa ttccgggtgc ttgaactttt
    13621 cctcctgcaa gaaaatacca tccatcagga tcaaccaata agttagcagt acctgctaaa
    13681 gctaatggtc ctatattttt atctaactgt aaattacctg tgaatcggct gtttacaaaa
    13741 tcatagttaa gtccaatatt tccaaatccg gaaggaatat tttttacttc aatattttga
    13801 ttacttgcat tgatggaacc cgtcactaca aatgtttttc ttgtatcacc ttgcattcct
    13861 ttgaagcctt gcatttcgcc actgaaagta aaatttttcc atattccgga actcatatcg
    13921 gcttccattt ttcctttaat gttggcaaga ctagtagtgc ttccacctaa tggtaatttt
    13981 tgattttcag gatcaacttg tatccatgct gctgtagtag ttttgtttaa aattgctttt
    14041 aaaaccattc cttctttttc ataaatagta ccaagtgcag tgaattttcc ttcagtaaga
    14101 ttttgcggac gatcattaaa ctgaacattt gcaacgaagt caacatctcc cggtcctttt
    14161 agtgatacat ttaatggata caactgaaat ttaatttgtc ctgcttcttt cgaaaattga
    14221 atgatgccgc tggtttcttt taactgtgga attccaaggt ctataccaca agtcatattt
    14281 acataatcct gtccactcat aaatgaaagt ggttttactt tcatcacact ataaaatgta
    14341 attgagtttg gttgattacc cggatttata atttgttctc cattgctgat taatgaaaga
    14401 ttttgaaatt tcatttcttc accggtcttc attccgggca atgttttaat gactacaccg
    14461 gggcttccat tctcacctat caaacgaaat tcataatgtg gtttctgatc tgagccaata
    14521 cttttgtttc gtccgaatac aggattggtt gatgttacat gaacaggaat gataccaccg
    14581 aatgaaagat tgtttacatc aaaatctcca atgccaatat gatccggctg taaagaaata
    14641 tcagatatgg gaatatctat acttccggtt ttaattgtgc ccgtagcaat gttaattgaa
    14701 ttatcgcctt gtgacagttg ccagttatta cctatgaatt gccacttttc taaattgaat
    14761 tttataggtt gattgccttt aagtggctcc aacttctcag gatgaattat gaggtttcct
    14821 gcatttatat cgagtgtaga aggagtcatg tccggcagtg cattgatatg aatgatggtt
    14881 tgtaaattaa tactgttgtc ctgtagccag gaattaatag catctgcctc tgcatcaaaa
    14941 ccaagtaagg aaaacttcat tgtttttcct tgcagattag ccaatccgaa tttcttttta
    15001 gtggaagttg caatatcaat cgaagaaata tcagttataa acgaacccgg tttatcagta
    15061 atcaacatag cttcattagg atcattattg aaagtgatat aactctgtgt aaatttaaat
    15121 gacgattttt gtacagctat ttttcctgtt aatctacctt tctgattatc ggattttacc
    15181 tgtaacacat cacctgaaga gggcttttga attactccaa aggaattttg caaaagcaat
    15241 ttaatatttg tgagatcggt tgaaaaggtg ttttccacag aaacaccaac aggaattcca
    15301 ttctttgtag catcactctt ttttattttc aaatcggtga aagatatttt ctgtttgctg
    15361 tcgtgcaatg aaaaattatc attagcaggc agatttatca aattaccact caccaaccaa
    15421 atgccgtccg attgtttcac tttggattga atgtccactt caaatccaaa aatattttca
    15481 ataatgagtt cattatcact aatcaaatta aaatccaatt cattctgctg acttaatgta
    15541 atttttttat tctggctgat gatgttgggc gtagcaccgg gtttacaagc ccaaactgtt
    15601 ttttctttcg gtgaagtaga aacaccttta agtacatatt ttccatcact gcctgttgtt
    15661 gtttcaatac tttttccatt acctgtttca atataaactt ttgctccgct taaagctttt
    15721 ttgtccgcac ctaaggttac agtgccggta attgttgcag cttttttcag atacgcattg
    15781 ttgtatgttg taatggttgt ggtgttcttt acaccggtta atttgtagtt tcctgtttcg
    15841 taatttgcat tttcaggagg agtaatgata aattcaaaat cagatgcact attatcaaat
    15901 ataaaagtca cataaccgtc tttatcactc atttgaataa ttggtttacc gggtatgtct
    15961 aactgaactg atgcacctgc aataggtttt gcattttctg cttcatgaac agttaatcgg
    16021 cttacggata aaagaggaaa ttccgctact ttaaatcgta ttcttttctg tgaacgtgtt
    16081 actacgaatt taatttctac aggattatta tcttccttct taatgacaaa cgtagtatct
    16141 tttagtgcat aagctttgtc aacaggagat actgttactt ttcttttacc ggaaggtgca
    16201 gccattgaaa aatattctct agtacctgta ggtacaacta tatttgtaat gctattatta
    16261 ccggaattat taggggcatt taacgggcct cgcttattat ttgagactct tctatatgtc
    16321 atattacctg aacttccata ggtaaactga aattttgttg aggtctttgt gtaaccatcc
    16381 acatcaatat cagcttttac agggttattg ttttcatcaa caacataacc actcaaaaaa
    16441 ccatccggtg taagaaaaaa gtcctgattt aatagctgtt ggccaaactt caaaggcgga
    16501 taacaagaag tacatgtagg aacaattttt tttcctttgt ttcctaaatt ccaaagtgaa
    16561 tccgattgtt gtgcacctgc tggtaacaca gcagctttgt atccatttgg ctgtactact
    16621 attgttcgag ttgggccaat gattgaaaca acttcaccta ctttaaactc ccctttctct
    16681 aaaggtaaat cattaaattc ataacgtcca ttttcatcag taagtactgt tgtaaacaat
    16741 tttgatgggt caacactttg tgtataggca ttaatcatga caacttttac attactcata
    16801 gtttttcctt ctatattaat agcatcttgc acttttcctg caattcttgg attatccgga
    16861 taaagcatca tactgtcggt atatgtcttt atttctaact catgattcca gataatagat
    16921 tcaccataag caacgggaag atttatacct ccttgtatat gtggtgtata agaaccggtt
    16981 gcattaaaag ggaacaaaag caattcctta tcatataatg gataataccg atccactctt
    17041 tctttaaaaa taaaatcacc ttttgttttg gatggagtgc acttaattga atagtaatcc
    17101 gatttgtcat cgggattatg ctgtactaaa tgtttaaaag ttacatatcc atctttgtcg
    17161 ctttcggctg atgcaatact gcttaaagca cctttcccat cgccttcatt ttttggaact
    17221 gatgaaggag gatttgattt tcgtaatagg gatgttgtaa ttccgggtaa tgcacttcct
    17281 tgtccatagg ccatatccca gtatttagca cttgttgata aaacttttac tttcagtgta
    17341 tagcttttaa catacgatac tacagttcct aagtctattc ctttccatgg atctattttt
    17401 atatttatat cggggctaca gtaatacctg tcttcaactc ggatgcgtaa tactttaaaa
    17461 atttttcctt ttacattatc tccaaattca ccggtatgtt taaatgtaaa attagaatca
    17521 attaagccta aaactttttc aacgtttaaa aaatcaaatt taaaactacc gtcagcaccg
    17581 gttactgtac tagcaagaac tttgtctgca tccggaggag aaaaaccgcc atgattgttt
    17641 atacctggct ggccatttaa gtttttacca ttgagtgttc cggtaaacag ataagttatt
    17701 acaaggctga ctttttttcc tgcaagaggt agtgcatttc ctaatgacgt attgtctgta
    17761 ttgtaaacaa gatcatcagg attagatgaa ccgccagcgg ttaataaagg attatttgtg
    17821 ttagcagttg gtccacctaa aataggttga ttggacacag atgctgatgt ataactatct
    17881 ttaaaactgt aaactaattt tccttttaat tgactcatgg gtacagggtc aagattggtt
    17941 acattaaatc ccggtgattg ttgtgatgga ttttttaaag ggtcttctat tttaatttgc
    18001 cctaccagcg gaatttctgt tttacctttt gtgaaagtca ccacttcgct tcgtccgtca
    18061 ttaacaaaac tcacttgttt atttaaatct cttgccacaa cctgcactgc atagagtgtg
    18121 tttgtatcta tttgcaaatc gtatggttgt gttacatatt gattgcctgt aagatttatt
    18181 tttttaactg gattgttagc attgtagttg accgcagcat tcataacatc atttgggttt
    18241 gagccttgtg gaatttttac aacatagagg tcataaacaa tattggctcc cattatatta
    18301 ccaataggtg gtgtccacga aaaaacggtg ttgggtaatt gaggattcag attctgccca
    18361 tcaaaaggag aaattatgat tggtgcctgt gcaagactta tgttgaattc atagcaagaa
    18421 ctacctgctg aaattaagcc tgcagccgaa gttatttcaa atgcctcaac acaaaaatta
    18481 taatgtccat cgggcagctg tcctgttttc aaaatatttg accacacagc atcaggaact
    18541 cccgaaggat ccacattatt cctgtttaaa aaaagcatag aaggaacatc attgattata
    18601 accttggtct gattaattcc tagattaaat gcaccaccca tgtatcctga tcttgtttga
    18661 ataaccaagt ctctgtctgt attaaccaat gtgccgtaca atacaatatc cattggtctt
    18721 gccccaccca ctaaagttat tattgcatgg ttcgataaat tttcatacaa actgtaatta
    18781 gaagaataag gtggcttgag tataacattt attgaagtca gaaatgaatt ctgactcatt
    18841 acccacatag gaaacattag taaataaaat ataaaataac ttttcctgaa tatgtttttt
    18901 gtgcctaaac tacaagtgtt cattgttact actttttaga aagttaatcc ataaataaac
    18961 tgtatgttgg tttctttaaa attctgtatt aaggaaacat cttttgaagt gttgtctaaa
    19021 taattacaca tcaattgaaa gttatgatgt gttcctactg gaaccataaa acctaatgta
    19081 aatcttatgg tattaccatt atcatttccc ttaaatttgt tttgcagata agagaaagaa
    19141 gtatttagag tgagtctttt tttcataagt ggtgctgatc caccaactga tccaccgata
    19201 gaacctacat caccagcggt agtttttgaa ttaataaaat tcatagcaac atttgcatta
    19261 ataccaggct ttatccaggt ttgagaatag ttagtagaaa aagaaaaagt attcatatca
    19321 ggtgtgttat tgccataact ctgtggagtg cttaaagatt gaaaagttgc aatacaattc
    19381 agatttttaa aagttttctc atttgtccag agtaaataag gcattagcat tacactgttg
    19441 ttaacctgtt tatactgttc attgccaggt gaagtctgca atgggctgtt ggtaatacca
    19501 aagttgctgt aattgaaact tattccaaat ttctgcgatg gaacataatt aacattaaca
    19561 cttccaataa atctttttga agtagaagac ttctgttttt tcaggttgtc ggtttgaata
    19621 ccaatgttgg tgtttatgtt caacctatta ctatccaact taaaattatt tgctaaactg
    19681 tactccttta tatcattctg aaaaaaatag gctcccatag attgataacc cgggtcaatt
    19741 cttttaagct tcaattgggc accccatttt ttttcctgat agccaatctt ggtttctcct
    19801 gcaaaataat attgtgtgct gagtttatca ctcactaaag gtcccataat atcttttggc
    19861 caagccttat caatatcact tgaatccttt ctgtccattc ggttaagtgt atatgcacta
    19921 aaaccaatat ctgatgtcca cgtaattttt ttcgataatg acaaagtgga aaccagtcca
    19981 aatgaagtgt tttctgcagg ttgaatagaa tcttttaatt ttccattaag tgaagaaggc
    20041 ttatcccatc ctttaaaata aaccagatca acataattct tttgtgagcc ataaccgatt
    20101 ttacccgcta tgccaacacg tttaaaagta ggtgttgaac gcacgtacat tgggtttgtg
    20161 ttcatagtgg aatcaataga tgctgatcgc cttagttgtc catacataaa tcctattcgg
    20221 aacttttttg gattcaaatc aaatcctaca cctaacattt tgtggccggc caacgtatag
    20281 tttgaatatg tgaggttacg atagcctaaa tgcatagtaa tccatttgta tcgtggactc
    20341 aatccaaatt gattaaatgg ctgcgaatat ttattgccaa aatttgaaaa aagaaaagta
    20401 aatggcaggt caattcccaa tacatttaaa acaggattgc cattgataat ccaagaaaat
    20461 tctttttgcc ttgcaggaat accattggca ctgtaataat ccaattgaaa attaagattt
    20521 ccatgaaatg ttacaggctt tttcagatta atgtgttcaa catcctgcgc cttactaatt
    20581 atggtaaaga ataaaaaaag taaaaataga gataatgaaa tctcataact ttttttttca
    20641 ttttgtgatt ttttctgcat gcggatatat tgaaaaaaaa tcaatcgtaa aaatcactca
    20701 attaagttgt ttaattctga tataccatta atcacatata aacaactcaa tcaatcacaa
    20761 attactttcg tgttagggta tttagaaagt aataagcaaa tgtaattgtt ttaagaaatt
    20821 aaaaaggaaa ttttctaaat atttttaact gagttataat tacatcctgt taaaattcct
    20881 atattgattc gaagacttat aaaggggtct ataataaatt tcttctaaat cacttaatca
    20941 tcaccccgcg tttttcgagt aagggtatct gtgtaaaaaa ttcttcattt ataactcccg
    21001 gaagaaaaac atactttcga tcgtacatgg tgttgttgag gtaaaagctt acccagtatt
    21061 cattattcag gttaagaaca ttatctgtaa taggttcaat ctttacgcac gactgcggag
    21121 gaatattttc tataaaatgt ctaagcgttg atgtttttct ttcttcacct tccatcatgc
    21181 catagccttt gcttgtaatc aacacacctt ccagcatttc atttttcagg ttaatgatat
    21241 aggcattcca aacatcggca ccgtttgatt gctcttttac aattgcaacg gctacgtttt
    21301 caacttttaa ttgaggaata tcttttagca tattatatta aacgagtaat gataatagaa
    21361 gttaccttga tagcttgtta ttcatacatt tttatgactt gttcggcata aaatttatag
    21421 tcattaggct ttccgtccgg attgttactg cgtttatggc ctaaacgcac aataatcatc
    21481 tgcatatcag gaataacaat aacatactgc cctaagatgc cacgcatgta atacacagga
    21541 tgattgctga tgtttgacaa ccaccactga tagccgtagt gctgaacagg tttattatct
    21601 tctttatccg gtaaattatt tggtgtggta cagagagtaa taaaagctga atcaataatt
    21661 tgttcattat tccatctgcc atgttgcaaa acaagtttac cgaaacgtgc aaagtcgcga
    21721 gcattgctgt aaaaacaaca ataagctttt tctattccgt ctttatgatc gaggctccat
    21781 tgtgcactat gttctgctcc caatggtttc caaagttttt ctgatgcata ttctgccaga
    21841 ctttttcctg ttgcattagt cacgatgata gctaacaatt ctgtgtcgca gcttttatat
    21901 tcccagaatt ttcccggtgc ttctgcaaca cctaatgagg caacctgttt gtaaagttgt
    21961 gttccataat aagcctccgt aacctgtgat gtcagcgatg aatatccttc attccagtca
    22021 agtcctgaac tcatcatcat cagatttcga attgtaattt ttgatttttc accctcttta
    22081 aattcaggaa taaagtctgc aacaggctga tcaaggcttt taatttttcc ctcgtcaatt
    22141 gcttttccaa tcagtaaagc aactatactc tttgccatag agaatgaatt ggaaagcgat
    22201 tgttgcccat aatcttccca atatttttca aaacaaatag aatcgttttt tatgactaaa
    22261 aatgcaacag atttattggc ttcgagcaat gcggttagtg tatcactcag atttttttta
    22321 ttgtagtctg tagaaacatt ccatggcatt cctgttccat tttttacttc tctgttgtga
    22381 aacaaattca aatcgtcaat gtcaacaaag ttgtaaacaa gagctttata gtaatacaac
    22441 tttcctgtag cggcaagtac tactgttgtt atcaataaaa taaacagaag gtatttgatt
    22501 atctttttca tgttttttct tttgctatta gtttttcctt tcctacattt ttaattgtgg
    22561 cattaaaaac ttcactgaaa tgttttagta agacagattt tacttcatca atatttaaat
    22621 tttttccaag ctcttttgcc attgatgtaa ctgctttatc gctgatgccg caggggacaa
    22681 tgtggttaaa ataatctaac tgtgtattaa cattaagtgc ccatccatgc atagttatcc
    22741 atctgctgca acgcacacca aaagcacaaa tttttcttgc actaggtttg cccgcatcaa
    22801 tccatactcc tgtaagtttt tctattctgc ttccctcaat gtcatattcc ttcaaagtac
    22861 ggataattac ttcttcgaga aaacgcatgt agcgatgaat gtcattaaaa aataagtcaa
    22921 gattcagaat agggtaacca acaatctgtc ccgggccatg ataggtaata tcgccaccac
    22981 gatttatttt gtagtaggtg gcattggcag ttttaagttg ttgttcgtta gagagcaaat
    23041 gttcgggctt tccgcttttt cctaaggtga aaacatgagg gtgttcacaa aacaatagat
    23101 aatgtggtgt tggtgcatga tccccaaaac cgtgattacg cccttctaat ttcagttgca
    23161 taatctgttg aagaagctgc tcctgaaaat cccaggtttg tttataatcc gatgcgccta
    23221 aatcagcaaa aagtatttcc tgcacttttg tttttataat ttacaaagat aaatgaatag
    23281 aagaaacgat ttttcagtga taaagtttgc ttactgcaaa gctaagacga atgatttcaa
    23341 aagctgaaat acattgttgt actttgttaa aggcaatgtt tctgctttat ccatcacatc
    23401 atgataccat gtaacaccac cgagggtata gataaaaaat gctggtacac ccttttcact
    23461 gaaataatag tgatcgctgt tttttgcttt acctctttta ccaatattgc taacaaattt
    23521 tgctatatcg ttgatagact gcaacaacga gaaatctttt tcaaactcag ttgcattgac
    23581 aaccataatg ccatcatcgc ctgtgccaag caaatcgaga tttatcagaa aacggatttt
    23641 tgatagtggt aatataggat gttctgtata atattttgac ccctgaagtc ctgcttcctc
    23701 tcccgcaaaa gcaataaata aaatggagta gggctgttgg ttttcttttt ttgaaaaata
    23761 ttctgctaaa gaaagtaaca tggcaacacc gcttgcattg tcatttgccc ctggaaaaaa
    23821 tgttttattt cccatacccc ctaaatgatc ataatgtgct gttatcacaa ttaaagaatc
    23881 aggtgtttta tttccagtaa ttaatccaat tacatttttt gaatcatagc ccgcatcaaa
    23941 tacggcatcg gcctttatgg taatattttt tgtctgacga ctgaacagac tatcatatac
    24001 ttcaacaaca ggaatattaa aaactgatgt accaacactc catgttagtt tatgcggttc
    24061 aagaattacc aatgctcctt tttttcgttt aaacagtaag gctgtaattt ctttttgctg
    24121 cttactgaaa acctgacgtg ttatgacata acaactgtca taatcattgt taagtcttga
    24181 aataatttct gcagaatctg cccaggctat aggccatgtt ccattggcag atggcgattg
    24241 atgatgtatt aaaaactctt tcccgggaat ccaatttttt gtgtctgtag ttagacttac
    24301 attttttgga aaacggttta caggatgtgt aaaatgctga aaccaatgtt tatcatcaac
    24361 aggttttaat ccaagtttgc tgaattgttt ttttatgaaa tgtgcagctt tcttatcacc
    24421 atgttttaaa tagccacgac caaaatattt ttctgaagtt aattcagtaa taacttttcg
    24481 cgcatcatct tgctgtgcaa aaacaaagtg tgtctgtaga aatgcacaaa taaaaatcag
    24541 cgatgcaaga gaaggtattc gcagcattac ataaatcgcc tttgtagcaa atctacaaac
    24601 tgtctgcagg catcactgtt tttgtctatt tgatttcctg aagttatttc agagtcgaag
    24661 tacatgtaag cttctgcaaa atttgaggaa gtattcaact tatcaattgc tgattgatat
    24721 tttatagaat catcattaaa caactgactg ataaataaaa atttttcgtt tatgcctatt
    24781 gctttaacca agtccgtaac tggtgatagt tttaacttat ctgctatggc aaaagccggt
    24841 tgcgccacac caatttatcg tgtatggttt ctgtttcgtg aaacatggtt gctacagttt
    24901 gatctgcttc tttaaatact tcagctcttg cagtttgtac aggaggaacc acagaagctg
    24961 taactttctt ctgtttcact tcttctgccg ttgttgtagc tgtatgaact tccggtttaa
    25021 tgggagtagg ctcagtgact tcagctatta tcggttcaaa ggtttttacc tccggtgctt
    25081 caatggttat tttttgttcc gtctcagatt tttcaatgac ggtttgtgtt attggttttt
    25141 gcactgtttc tgtaattact tcctgaatag gctgcttata agcataaaca gattcaagta
    25201 attgaagatg attgatttta tcgtgcagct tcatcagttt gcttttcagc atgtctaatt
    25261 catcattttc aggaatgcgg ttgtctaatg aaaaaacgcc ccattgcctg taaatttgtt
    25321 ctattgcaac ttgaatttcg ctggtgatag attgctttgt catgaaattg tgtttttatg
    25381 ctaacgctaa ataaattaat tcatttttga tttggaaaat ttatgggtaa tctcagaaaa
    25441 ttttcatgag caaaattaat tgtcatacta ctgtcaacat tgttggttga ctgatattta
    25501 tcaacataac ttttccatgc tgcaagtcgc tacttttact
//
LOCUS       DAOOOL010000019        25247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_152_length_25247_cov_59.741743, whole genome shotgun sequence.
ACCESSION   DAOOOL010000019 DAOOOL010000000
VERSION     DAOOOL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25247
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_25247_cov_59.741743"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1310
                     /locus_tag="PKU82_02880"
     CDS             177..1310
                     /locus_tag="PKU82_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOZ81848.1"
                     /translation="MKGQETFFISCAPHVVPWLKQELDDLGYMPFEENYTGLFIKAPF
                     EKCMELNFQLTTASRVLWLVDKFTATDQKQLYNKLSRIRWSDYFSEKKYITITSQTNH
                     PSINNSMFLNQLCKDAVVDFFQSHYNIRPDSGPLRSEVVIHLHWQENKAWVYLDTSGE
                     QLNKHGYRQNPFKAPVQENLAAAIIKATAWDKKCTFINPMCGSGTFAIEAAMMAGNKL
                     PLLSRSNFGYMHLFDFNKKKFELLRSELALKSNFENMPAIIASDNNGYAIKATRENAE
                     RAGVDAFIEMTKCDFTETSIPDGNGVIVINPPYGERLGENIDLEALYKSIGDFFKQQC
                     KGKTGFIFTGDLNLAKKVGLRTSARIPFYNGTIECRLLKYDLY"
     gene            complement(1313..1930)
                     /locus_tag="PKU82_02885"
     CDS             complement(1313..1930)
                     /locus_tag="PKU82_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOZ81849.1"
                     /translation="MFETSYKTIVGYSEGVFRDKGSRFLVFAYYVEKESECKSVLEQI
                     KKEHPKANHHCYAYRLGPYKNIFRWNDDREPSNTAGRPIYAVIQSNDLTNILIVVVRY
                     FGGTLLGVPGLIQAYRSAAEATIEQNTIIEKDIEEEFELQMSFEEMAEVMKIVKQHKA
                     TFTQTSTANTLTLVVKAKKTFSDQFLKAINGNYLLKDKVKIRAIS"
     gene            complement(1931..2791)
                     /locus_tag="PKU82_02890"
     CDS             complement(1931..2791)
                     /locus_tag="PKU82_02890"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOZ81850.1"
                     /translation="MTVTVFMAVLFASLLFVILKLFDRFKVSNIHGLTFNYFTAGICY
                     TLYSKQTYSSISGFAASYFPYYLLSGSLFIGVFYLTALSSQKNGVASTTIASKMSLVI
                     PALLAVFLYNETFSFLKTIGLLMALSAVYLSARGSNNESSESGWTLPLLVFAGAGLVD
                     SSIKYTQHVFTDNAGSSGFFTLVFFSAAIIGSCVLFFSKNMPKEGSLFRNIIAGIILG
                     IFNFISLYYMVAALQEFHEDSGKAFILINSGVVLLNFLLSIILFQEKKNTQSWVGVAL
                     AVVAIIILSQ"
     gene            complement(2788..3819)
                     /gene="ribD"
                     /locus_tag="PKU82_02895"
     CDS             complement(2788..3819)
                     /gene="ribD"
                     /locus_tag="PKU82_02895"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOZ81851.1"
                     /translation="MTFDETIMNRCLELAKKALGQTMPNPMVGSVLVFENKIIGEGYH
                     HRAGEAHAEVNAINSVENKNLIPLSTLYVSLEPCSHYGKTSPCCERIVQEGIKKVVVG
                     TLDTTAKVNGKGISYLQQNNVEVATPMLNDACRFLNRRFFTFHEKQRPYVILKWAESA
                     DGFIAPENAQQQYAISNTYSKTLSHKWRTEEQAILVGTKTAILDNPQLNPRLWKGSTP
                     IRVVIDKNLDVPATHHLLDGSVKTILINQKKENRANNIDYIKINFQENPATQILKVLY
                     QQNIQSVIIEGGAKTLQYFIQSNLWDEARVFTATKQLISGIKAPVFLISPSEITNIED
                     DTLKIFYNV"
     gene            3849..4712
                     /gene="prmC"
                     /locus_tag="PKU82_02900"
     CDS             3849..4712
                     /gene="prmC"
                     /locus_tag="PKU82_02900"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOZ81852.1"
                     /translation="MMRLFSFATMTRKEIINVFQADVELIYDKGESSAITKLVMEYIF
                     GLNYKSNTHENVNVSQETLLRQYLERLRNYEPVQYILGQAWFCNHIYKVNSSVLIPRP
                     ETEELILLIKQNLKSSHIGSIIEIGTGSGCIAIELSLLFAQATVIATDISENALEMAR
                     KNAESLRASVSFINESLFVPSSKTIQTKVDLIVSNPPYITQQEATQMHPNVLQWEPHT
                     ALFVKENPLEFYIAIASLATNILNDKGYVFLELNPDYAFQVAQCFTEKGFTQVEIVKD
                     ISGKERFLKVQ"
     gene            complement(4730..7568)
                     /gene="uvrA"
                     /locus_tag="PKU82_02905"
                     /pseudo
     CDS             complement(4730..7568)
                     /gene="uvrA"
                     /locus_tag="PKU82_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            complement(7694..8209)
                     /locus_tag="PKU82_02910"
     CDS             complement(7694..8209)
                     /locus_tag="PKU82_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81853.1"
                     /translation="MRIWGLKLLVLIWVVFTGYWLLPLKKPKAYRQDSEPNTILATQQ
                     FCEPECADIIIKKGELIIPDSLKKMYPELRKDVALLVGSSPFKKSKSALMFSYDFVLS
                     GHVERVGFTQQEGFVPVFHVEEWFPTQYIARFWKLTHNSELLYLINLNIGLPLLLFYF
                     FKQGSSLNKFF"
     gene            complement(8223..10292)
                     /locus_tag="PKU82_02915"
     CDS             complement(8223..10292)
                     /locus_tag="PKU82_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ81854.1"
                     /translation="MKTIQLFFISVLFCNITLAQVVPPQDDSKIEEKVENIANTADES
                     ADLTELTEQLQQLAANPINLNKATREDLLQTGLFSEVQADAIIEHREISGKFISLYEL
                     QAIDFLSLEDIEEILPYVTVGNTEDIKATLKKIFTDGNSRLIVRGQRYLEKQKGFRDS
                     TATDKVHPAYPGSPWKLYTQYQYRYKQRFSFNLTAEKDQGEEFFKGSQRNGFDYYSAH
                     LAIRDFGIIKRAVIGDYDLFYGQGLTLCTGLSFGKSPDVAAVRKFSTGIRPYTSLNES
                     SFKRGVAVSVGKRNWNADAFYSNRKLDANLNSILDTTLEQQDIFTSFIESGYHRTKSE
                     LENKNTIREQMYGVNAGYNTRNFTAGLTAVHAGYDRPLQRSFQLYNRFDFSGKQFTKV
                     GIDYSWLYRNINFFGEFSRSDNGAIAYVNGAIISLGTTVSVSVVNRNYPRDFNFLYAS
                     GFGESSTTRNEKGTYAGIVIRPNRYWGFNGYLDRFTFPWLRFGADAPSKGYEYLWQVS
                     WNPSRQFEIYFRNRRTQKEENSSAAAVADYLVFKRQDNYRFNLTYKISKAVAIRNRIE
                     WVRINYEEKPNEKGFMIYQDILFKPLSSPISGSVRYGLFDTDSYDSRIYAFENDILYS
                     YSIPSFYNRGFRYYVTLRYKVTKGVDVWFRFAQTVYTNQKTFGSGLDEINNNKRSEIK
                     LQVRYQF"
     gene            complement(10295..11107)
                     /locus_tag="PKU82_02920"
     CDS             complement(10295..11107)
                     /locus_tag="PKU82_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81855.1"
                     /translation="MKPTILAILLFCFSSVSAQTYLTSGGARASAMSDAAVTFNDVFA
                     AFNNQACLSMLEKPEAGLAIQNRFGMKELNTLSAAFALPLASNKGVFALSINRYGYKL
                     FNQNKIGLAFAKKLSPVFSAGVQVDYLNTHIDEGYGNKSSFTIEGGVLAEFNKLKAGV
                     HIFNPLHVKLTDYDDERIPVIIKAGISYSFSEKVLAAAEIFKSIDRKETYKIALEYHP
                     VKALYVRGGVSTEPVQLTFGFGLKFSQFYADFTSGYFEPLGYSPGLALRYAF"
     gene            11339..12148
                     /locus_tag="PKU82_02925"
     CDS             11339..12148
                     /locus_tag="PKU82_02925"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81856.1"
                     /translation="MKTITYCFAIIVFSCNMTFAQNCDLLCDGDFENPTVISSPHLVN
                     YKTIECWKTTASDSILEFWDSGFNGVPSFSGNQFLEINANEVASIYQDVYLTAGDVIT
                     LHFAHRARVGIDTLGVSVSFQNNTPVILGYYADDDLVWGYYTEQYTVPATGQYRISFI
                     SVSNFYNVPTLGNFIDAVEVCLSPVGFDEVNLQETFLISSQKSTLKIDFTSQLKGEGI
                     LTVFDLQGKEVFRISVDNSRKQIATAPLNSGIYTVQLIQGRSITAKKVLIN"
     gene            complement(12145..12876)
                     /locus_tag="PKU82_02930"
     CDS             complement(12145..12876)
                     /locus_tag="PKU82_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ81857.1"
                     /translation="MSDRISNELEHGKKLLEGGAEAIWNWDSPAGKVRADRRADLIAK
                     YSLADKSSTVLEIGCGTGLFSGKFYQRTGAKIIATDLSPELLNVAKEKYPAVEFVLAD
                     AMKLQFDANTFDVVFGSSVLHHLEFERSLNEILRVLKPGGRMVFAEPNMINPQIFVQK
                     NIPFIKKWLGDSPDETAINRWNFSKLLEQKGFNQINIFPYDFLHPVVPSFLISLVSGI
                     GQVVEKIPIAKEIAGSVIIVAEKPR"
     gene            complement(12876..14543)
                     /locus_tag="PKU82_02935"
     CDS             complement(12876..14543)
                     /locus_tag="PKU82_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81858.1"
                     /translation="MKKNKEVVAKKSSTKTVNKPSRETTLIKLENFLQKNETIIFFSL
                     LLVSLIFSVLLFDIKMSEGNDDSDYIEGAFKLTKDFSSYYSTKAPLYPIFLSLPVGIW
                     GINLPILKSLSILFSLLNLTAMYYAFRKLIPYTILFAVLIFTGINSYFLYFASQTYSE
                     AFFCFLQALLFIVIRPILSASKEDNIPIAKYLVFGIILTLLSLTKNITVGAIAAVALY
                     FVLNRDFKKLAYAFGSFVVVRGIFEVIKSFIWGSQNQFSNQYQILIQKDPYNASKGVD
                     DFGGFVDRLFSNTSIYIGKRLYQILGFVSPDNTKTNAGLVLITFVFVGIGLYFIFKNK
                     NKIMQFTFLYVLLMAGLTFFVLQTSWDQPRMVLVYVPFILMIIFYGLYYAFQNTPALR
                     SSIFLIVSIIIIMSSLMKTVDKSVTNLPILKKNMGGDIYYGYTEDWTNFLKLSRYCGD
                     SLPESAYVVSRKAPMSFIYSKGKSFYPIYTVPSTDPDSVLALLKENKVTHVLIASLRR
                     NPKKNDGYVINTLHRMMGPVGQKYPDKLKLVKQVGTSEPSYLYEIKY"
     gene            complement(14558..15211)
                     /gene="fsa"
                     /locus_tag="PKU82_02940"
     CDS             complement(14558..15211)
                     /gene="fsa"
                     /locus_tag="PKU82_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOZ81859.1"
                     /translation="MKFFIDTANLAQIKEANDLGVLDGVTTNPSLMAKEGIKGKAAVM
                     KHYVDICNIVKGDISAEVIATNYDGIVAEGEELAKLHEQIIVKVPMIKDGIKAIHYFT
                     SKGIKTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDVSTDGLQLISEIRQIYDNYGF
                     QTQILAASIRHAMHIINCAKLGADVATCPLNAITDLLKHPLTDIGLEKFLADAKKFV"
     gene            complement(15236..15805)
                     /locus_tag="PKU82_02945"
     CDS             complement(15236..15805)
                     /locus_tag="PKU82_02945"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOZ81860.1"
                     /translation="MLNIVLFGPPGAGKGTQSEKLIQQYGLIHLSTGDLLRNEIAGQT
                     KLGLEAKKLMDQGVLVPDEVVIGMIESKLDANPTAKGFIFDGFPRTTAQAAALDNLLK
                     QKNTSIKMMLALEVTEAELSKRLLLRGKDSGRPDDQNPEIIKKRINEYNNKTAPVAEY
                     YRMQKKYYGINGIGSIDEIFSLLCKAIDK"
     gene            complement(15865..16401)
                     /gene="hpt"
                     /locus_tag="PKU82_02950"
     CDS             complement(15865..16401)
                     /gene="hpt"
                     /locus_tag="PKU82_02950"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOZ81861.1"
                     /translation="MSEVTILDKTFTIMIPRKQIETRILEIARDLNAEYKDKNPVFIG
                     VLNGAFLFMADLFRNIETPCEISFIRVSSYSGTESTGQMKNVVGLSGNINNRHVIIVE
                     DIVDTGETVSYLMQEIAKHQPASVKLATLLFKPQALKKKVTVDYAGFEIPPAFVVGYG
                     LDYDGYGRNLQDIYVLKS"
     gene            16528..17658
                     /locus_tag="PKU82_02955"
     CDS             16528..17658
                     /locus_tag="PKU82_02955"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOZ81862.1"
                     /translation="MNAFNPNIVIGVLGGGQLGRMLLQKAADYNLSIHILDPDEAAPC
                     RYMCKTFEHGDFRDYQAVMAFGNDCDIITIEIEHVNIEALYELEKLGKKVYPQPHILE
                     MVQDKGLQKEFYQKNNLPTAPFYLVEKPEQIKPEFPFVQKLRKGGYDGKGVLVVKDAD
                     GMSQLFQAPSVIENKVVIEAEIAVITARNNSGEIKSFPVVEMQFNAEANLVELLFSPS
                     KTSAVIQQKAVSIAEDLISKLNMVGLLAVEMFVTKDGQVLINEIAPRPHNSGHHTIEA
                     NHTSQFEQHLRAILNLPLGNSSTILPAAMINLLGDKDSNGRAIYSGVQDALNAGDVHI
                     HLYGKKITKPYRKMGHVTVTAATVDEAILNAQKIQSQLKVIS"
     gene            complement(17651..18469)
                     /locus_tag="PKU82_02960"
     CDS             complement(17651..18469)
                     /locus_tag="PKU82_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81863.1"
                     /translation="MENNKMNKLFDAARNADPVVSLDESLEQFNKEVEGKETTLGYMK
                     WLKPVIAVATIAVVGGGIYFLVQNQGQQKTQPTQENTEQTTQPALQATETQPDAAIEQ
                     PQATEENLTETPSVKTEITNPAPLNNKVTHAASANDAYIVETQTVNINNDNGAFKISI
                     SGDDVERIVLNGKEVEKSDWSKYQDEVAEAMAAKSSMGINKENAANKKFVDYMFTTLK
                     ENGLIKNNLAVIKLSNDMLLIEGQEADSAIHQQLLKEYKKITGKDIGLRTLYFN"
     gene            complement(18491..19009)
                     /locus_tag="PKU82_02965"
     CDS             complement(18491..19009)
                     /locus_tag="PKU82_02965"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ81864.1"
                     /translation="MSGQEKISGKQKAFTDAYQQLHEKLVRFVQSMVYNREDVKDIVA
                     ETMLVAYEKFETIRHKDALLSYLFTVSSRLVYKLQDKKKQIYTAFDPEQAEQVSDDHA
                     GADSKVQLKELYNALDQLPLKQKEAIVLFEISGLSLAEIQQIQGDSLSAVKSRIARAR
                     ESLKKILEEEIV"
     gene            19119..20066
                     /locus_tag="PKU82_02970"
     CDS             19119..20066
                     /locus_tag="PKU82_02970"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOZ81865.1"
                     /translation="MQALSSTNFKFQGQTAFYKGKVRDVYSIDNKWLVMVVTDRVSAF
                     DVVLPRPIPYKGAVLNQIAAHFMQATADLVPNWIVDTPDVQVSIGIPCQPLRVEMVIR
                     GYLSGHAWREYKQGKRMLCGVKLPDGLNESDALPEPIITPTTKAAHGHDQDISKEEII
                     KQGIVDAAIYEQAESYTKQLFRRGSEMAAQRGLILVDTKYEFGVHQGKVYLIDEIHTP
                     DSSRYFYADGYAERQKRKQPQKQLSKEFLRQWLIENNFQGLAGQTVPEMSDEIVQMVS
                     DRYIELFENITGKTFVKPSNENIEQRIYGNIVKSLEKLT"
     gene            20213..20809
                     /locus_tag="PKU82_02975"
     CDS             20213..20809
                     /locus_tag="PKU82_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81866.1"
                     /translation="MKKQIASLLFIAAVVSTASAQKIKILDGSLAGLKGETKLNVVYD
                     YSNMGVGSFAKEEEYVAKKTREYNAKEPGRGDSWAKSWVADRKNRFEPSFVELFNKKS
                     PMLIGDFPDAKYTMKVSTTFTEPGFNVYVTRKNASINLEITVYETANPDKPICKMQGL
                     RFPGRTFGGYDYDTGTRISESYAKAGKDLAAFIGKNLK"
     gene            complement(20884..21999)
                     /locus_tag="PKU82_02980"
     CDS             complement(20884..21999)
                     /locus_tag="PKU82_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOZ81867.1"
                     /translation="MLIDVLHQRILGNTIEDYLWFAGLILAGLVFKNLISRILTFLLF
                     NVLKKYFKTIGVNKFVELTQKPFSATLFLIIIYIACRYVHFPDEWKLASESEFGVRMT
                     IKLIFQIVTVFFITWTILRVIDYIGLVMRERANKTESKMDDLFVPFFASSMKAITVII
                     SLFFILGAVFKLNVASIIAGLGIGGLAIALASKDTLENLLGSFLIFMDKPFVVGDLVK
                     TDSLRGHIEEVGFRSTKVRTIEKTLVIVPNKKMMDAELENQTERTFYRHRFVIGLVYS
                     TTADQIQNIIQQILNMLNEHPLVNENPNVFFTNFGASALEIEVIYFVKTQELSDFENT
                     RQEVNFGVMKIVAENKSDFAFPSTSVYIEKEVKKFLQ"
     gene            complement(21999..23225)
                     /locus_tag="PKU82_02985"
     CDS             complement(21999..23225)
                     /locus_tag="PKU82_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOZ81868.1"
                     /translation="MLDLLKKFEEKRPEIVFEWSDAETGAEGWLVINSLRNGSAGGGT
                     RMRKGCDKREVESLAKTMEVKFTVSGPPIGGAKSGINFDPADPRKNQVLERWFKAIKP
                     LLKTYYGTGGDLNVDEIHDVIPICNKLGILHPQEGTAVGHFNDGETNIKNRISNLQQG
                     VSLVLKDASLSPDLSHKYRVADMITGYGVASAVKYYYELWGGKISEKRAAIQGWGNVA
                     AAAGFYLAQMGVKIVGIIDRDGGLINENGYTFEEIKTLFNNRQGNKLVADNMLSPQQM
                     AEKVWNLDAEIFIPGAASRLVTQEQMQSLINKKLEVISCGANVPFADKEIFFGPIGLY
                     CDKHVSVIPDFIANCGMARVFAYLHNANAVIDDRLIFDDAATTIKNALQEVIATHKDK
                     KGIASAAFEIALKKLL"
     gene            23359..23541
                     /locus_tag="PKU82_02990"
     CDS             23359..23541
                     /locus_tag="PKU82_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81869.1"
                     /translation="MRNQRFEQKKSIKDHWETDGHHAKKQKMANHREEKNWKNKILKE
                     EDDDSDEDFFRYDEDE"
     gene            complement(23578..24651)
                     /locus_tag="PKU82_02995"
     CDS             complement(23578..24651)
                     /locus_tag="PKU82_02995"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOZ81870.1"
                     /translation="MAASLKVLGLMSGTSLDGLDLALVNITEANDGLHYEIVAAETIE
                     YDQKRRDELANANLLSGEKLFELHHKFGVFLGESSLNFLQKHQLSCDLIASHGHTVFH
                     QPANGFTTQIGHGADIAAIAGITTVCDFRSLDVASGGQGAPLVPIGDELFFGMYDYCL
                     NLGGIANISCKVKNSRIAFDICPVNMALNYLANSGGLKYDAEGKLAAAGKLNTTLFDK
                     LNALEFYRKPYPRSLGAEWFNEYFLPIINSSTGTLNDKLHTITQHIAYQISQALTLAG
                     NNPNTTALITGGGAKNKFLIQKIQEKTTVKIIVPDVKIIDFKEALIFALLGYLRINNN
                     KNTLASVTGAKKDSVGGAVYLMT"
     gene            24764..>25247
                     /locus_tag="PKU82_03000"
     CDS             24764..>25247
                     /locus_tag="PKU82_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81871.1"
                     /translation="MMASTGNKQNTDTAGIDSEVLYNRILFAVIAMCYLLFGIFFSEK
                     VIDVLLPLTWMALVPLALLAVSFFLPVAKKYSGEQLSGMFLLLTIHLMFFLFRNKFSG
                     HPEFLLVTVILFSNLHIRNVIYLLLYNVIVLGSLEYILISGTSGSSDPVRLFFIIVIA
                     M"
BASE COUNT         7901 a         4617 c         4479 g         8250 t
ORIGIN      
        1 tgattgtttg gtttttgatt ttctgtcatt agtgcaaagt taagccagag cgaatacaat
       61 attacaacgc agaaatgaaa tgtttaatat tttaagcaac gtttttaaca tcacatgtta
      121 aactttacct atgctgatga tactaaaacc tacctttgcg gcaaaataaa ttttcaatga
      181 aaggtcagga gacgtttttt atcagttgtg cgccacatgt tgtaccctgg ttaaagcagg
      241 aattggatga cttaggttac atgccttttg aggaaaacta tacagggtta tttattaaag
      301 caccctttga aaagtgtatg gagctaaact ttcaactcac cacagccagc agggtacttt
      361 ggctagttga taaatttact gcaacagatc agaagcaact ttataataaa cttagccgca
      421 tcagatggtc tgattatttt tcagaaaaaa agtacatcac cattacttcg caaactaatc
      481 atccgtcaat aaacaacagt atgtttctga atcagctttg taaagatgca gtggttgatt
      541 tttttcaaag tcattataat atacgccccg actcaggacc attacgtagt gaagtggtta
      601 ttcatctgca ttggcaggaa aataaggcat gggtatatct cgacacctcc ggtgagcaat
      661 taaataaaca tggttaccgt cagaatccat ttaaagcacc ggtgcaagaa aatctggcag
      721 cagcaattat caaagcaaca gcgtgggata aaaaatgtac atttattaac cccatgtgtg
      781 gtagtggcac ctttgcaatt gaagcagcaa tgatggccgg aaataaattg ccactattgt
      841 caagatcaaa ttttggatac atgcacttat tcgattttaa taagaaaaaa tttgagttgc
      901 ttcgtagcga acttgcattg aaaagtaatt ttgaaaacat gcctgcaatc attgcatcgg
      961 ataataatgg atatgcaata aaagccacac gcgaaaatgc agaacgtgcc ggtgttgatg
     1021 cttttattga aatgactaaa tgtgatttta cagaaacctc aattcctgat ggcaacggtg
     1081 taatagtaat taatccgcct tatggtgaac ggttgggtga gaatatagat ttggaagctc
     1141 tttataaatc aattggagat tttttcaagc agcaatgtaa aggaaagaca ggttttatct
     1201 ttacgggcga tcttaatctt gctaaaaagg tgggactgcg aacttcagca cgcattccat
     1261 tctacaatgg aactattgag tgccgcttgc tgaaatatga tttgtattaa aatcaagaga
     1321 ttgctcttat cttaacttta tctttcaaaa gataatttcc gtttattgct tttagaaact
     1381 gatcagaaaa agtttttttt gcttttacta ctaaagttaa agtgtttgca gtagaggttt
     1441 gtgtaaacgt tgctttatgt tgttttacta ttttcatcac ctctgccatt tcttcaaacg
     1501 acatttgaag ttcgaactcc tcttcaatat ctttttcaat aattgtattt tgttctattg
     1561 ttgcttctgc agcacttctg taagcttgta tcaagccggg cacaccaagt aaagtaccac
     1621 caaaatagcg gacaacaaca ataagaatat ttgttaagtc attggattgg attactgcat
     1681 aaataggacg accggcagtg ttggaaggct ctctgtcatc attccagcga aaaatatttt
     1741 tataaggtcc taaacgataa gcataacaat gatgattggc ttttggatgt tcttttttta
     1801 tttgttcaag aacagactta cattcacttt ccttttcaac atagtaggca aaaactagaa
     1861 acctgctgcc tttatctctg aaaacgcctt cagaataacc aacgattgtt ttatagctcg
     1921 tttcaaacat ttattgcgac aatataataa ttgctacgac tgcaagtgca acaccaaccc
     1981 aactttgtgt atttttcttt tcttgaaata aaataattga aagtaaaaag tttaacaata
     2041 caacacctga gttaattaaa ataaatgctt taccactgtc ctcatgaaat tcctgaagtg
     2101 cagcaaccat ataataaaga gaaatgaaat taaagatacc taaaataatt cctgctatga
     2161 tatttcggaa aaggcttcct tctttgggca tatttttact gaagaaaaga acacaactgc
     2221 caattattgc agcagaaaaa aatactaatg tgaagaagcc actactgcct gcattgtctg
     2281 taaaaacatg ttgtgtgtat tttattgaag aatcaacaag accggcacct gcaaatacca
     2341 ataatggtaa tgtccatccg gattctgatg attcattgtt actgcctctt gctgataaat
     2401 atactgctga tagcgccatt aataggccga tagttttcag aaaagaaaaa gtttcattgt
     2461 acaggaaaac agccagcagt gcaggtataa ctaaagacat tttgctggca atagttgttg
     2521 aagcaacacc attcttttgt gaagataaag cagtaaggta aaatacacca ataaacaaac
     2581 tcccactcag aaggtaatag gggaaatagc ttgctgcaaa ccctgaaatt gaactgtagg
     2641 tttgttttga atataaggta taacaaatac cggccgtaaa ataattaaat gttagtccgt
     2701 gaatgttgct gactttaaaa cgatcgaata gtttaagtat gacaaagagg agtgaggcaa
     2761 acagtacagc catgaaaaca gtaactgtca tacgttgtaa aatattttta atgtatcgtc
     2821 ttcaatgttt gtgatttcgg aaggtgaaat taaaaataca ggtgctttta ttccgctgat
     2881 taattgttta gttgcagtaa acactcttgc ttcatcccat aaattgcttt gaataaagta
     2941 ttgcagtgtt tttgctccgc cttcaattat tactgattgg atgttttgct gatataatac
     3001 tttcagaatt tgagttgctg gattttcctg aaagtttatt tttatatagt caatgttatt
     3061 cgctctgttt tcttttttct gatttattaa aattgtcttt acagaaccat cgagcaagtg
     3121 atgtgtcgcg ggcacatcta agtttttatc aatgacaact ctaattggag tacttccttt
     3181 ccaaagtctg gggttcaatt gtgggttgtc taagatagct gtttttgttc ctaccaatat
     3241 agcctgctct tctgttcgcc atttatgaga taatgttttg ctataggtgt tacttatagc
     3301 gtattgttgc tgcgcatttt caggtgcaat aaagccatca gcactttctg cccattttaa
     3361 aatgacataa ggtctttgtt tttcatgaaa agtaaaaaac cttctgttta aaaaacgaca
     3421 tgcatcattg agcattggtg tagcgacttc aacattattt tgctgaagat aacttatgcc
     3481 tttgccattt acttttgctg tagtatcgag ggtgccaaca acaactttct taattccttc
     3541 ctgaacaata cgctcacagc aaggtgacgt tttgccgtaa tgcgaacatg gctctaatga
     3601 aacataaagc gttgaaagag gaataaggtt tttattttct acactgttta ttgcattgac
     3661 ttctgcatgt gcttctcctg cgcgatgatg ataaccttca ccaataattt tattttcaaa
     3721 aacaagaaca ctgcccacca taggattagg catagtttgt cctaaggcct tcttggctag
     3781 ttcaagacaa cgattcatta ttgtttcgtc aaaggtcata accatttgtt gctgcaaaat
     3841 taaaataaat gatgcgttta tttagctttg caacaatgac aagaaaggaa attattaatg
     3901 tttttcaggc agatgttgaa ttaatctatg acaaaggtga aagtagcgcc attaccaagt
     3961 tagttatgga atatattttc ggcttaaact acaaatcaaa tactcatgaa aatgttaatg
     4021 tgtcacagga aaccttactt cgtcagtacc tggagcgttt acgaaattac gagccagtac
     4081 aatatatttt aggtcaggca tggttctgta atcatatcta taaagttaac tcaagcgtac
     4141 ttattccacg accggaaact gaagagttaa ttctgcttat caagcaaaat ctgaagtcat
     4201 cacatattgg gtcaataatt gaaataggta caggctccgg ttgcatagct attgagttgt
     4261 cattattatt tgcacaggca acagtcattg caacagatat ttcagaaaat gcacttgaaa
     4321 tggcaagaaa gaatgctgag agtttaagag cttctgtttc attcatcaac gaatctctat
     4381 ttgtgccaag cagcaaaacg attcaaacta aagtagattt aattgtttct aatccgcctt
     4441 acattacgca acaagaagct actcaaatgc atcccaatgt attgcagtgg gagcctcata
     4501 cagctttatt tgtaaaggaa aatccgcttg aattttatat tgcaatagca tctcttgcaa
     4561 cgaatatatt aaatgacaaa ggctatgttt ttttagagct taatcctgat tatgcatttc
     4621 aggtagcaca gtgttttaca gagaaagggt ttacccaagt tgaaatagta aaagatattt
     4681 caggtaagga acgtttttta aaagtacagt gattaagtaa aaaataaaat cagacttccg
     4741 cactgacttt attgtgccat tccatttctt ttttcaaaaa agttcctgta atgctgttgg
     4801 cgtttttact gatttgtaat ggtgtgccct ctgcaacaat atttccacct ctcattcctc
     4861 cttcaggacc aagatcaata atataatcag cagctttaat aatatccatg ttatgttcaa
     4921 taactacaac ggtgttgcct ttgtcaacca attgctgtaa aaccaaaagt aaaattctta
     4981 catcttcaaa atgcaggcct gttgttggct catcaagaat ataaaaagta ttgcctgtat
     5041 ctttttttga aagttccgat gctaacttca cgcgttgtgc ttcaccacct gatagggtag
     5101 ttgatgattg ccctaatgtt atgtaaccta aaccaacgtc ttgcaacgtt ttaattttat
     5161 gcaagatagc gggaatgttg ctgaaaaaat caacagcttc attgatactc agattcaata
     5221 catcgctgat agatttacca cgataacgaa cttcaagtat ttcgcgtttg tagcgtttgc
     5281 cgttgcaagt ctcacaatgt acgtgcacat caggcaaaaa gttcatctgt ataattttca
     5341 agccagcacc cagacaagta tcacaccggc caccactaac gttgaatgaa aaaactcccg
     5401 gggcataacc tcttattttt gcttcgggca attgtgaaaa caaatttctt atgtcagtaa
     5461 aaactcctgt gtatgtagca ggattactac gtggtgtacg gccaattggc gattggtcaa
     5521 tctcaataac tttatcaata tgactcaatc catttatttt ttcataaggc aatggttttt
     5581 gcgtgctgtt ataaaaatgt ttgttcaatg caggataaat agtttcatta accaaggttg
     5641 acttgccgct tcctgaaaca cctgtaacgc aaattaatgt tcctaaagga aaaagtgcag
     5701 tcacattttt tagattgttt cctttagcac ctgcaatggt taattttttt ccattgccct
     5761 ttctgttttt tgaagaaggc atcttaatgc caccactcat ataatttgct gtgagtgatt
     5821 gctgatactt tagctcttca ggcttaccta ttgctacaat gtcaccaccg ttaatacctg
     5881 caccggggcc aaggtcaaca acataatcgg cagcttcaat agtttctctg tcatgctcaa
     5941 caacgatgac ggtattgccg atatcgcgaa ggtctttcaa tgcttttatc agtcgttcat
     6001 tatcgcgctg atgcagtcct atagaaggtt cgtcaagaat atataacaca cctactaatt
     6061 gcgaaccaat ttgtgtagct aaacgaatac gctgcgattc accaccggac aaacttcttg
     6121 tgctacggtt taaggtaatg taatcaagtc ctacatcaag cagaaaacct aaacgcttta
     6181 caatttcttt aagtacttct gtagctatga tattttgttt ttcactcagt gaagcaggta
     6241 gctgttgaaa ccattgctgc aactcaccaa ggtccatcaa agaaagttca gatatatttt
     6301 tattgttgag tttaaatgca agtgattctt ttttcaatct tgtgccttca cattctgggc
     6361 aaataatagt ttgcataaaa ttcaacgccc actttttgat ggagtaagat gcattttctt
     6421 ccatctgatt gacgataaag tttacgatac cttcaaaagt aattccatga ctgctgcccg
     6481 gagttgcatt gagtgaaaca tcatctgtgc cgtacaatat tatgttcagt gcttcatgcg
     6541 gaatatcatg aattggtgtg ttcaggttga agccaaacct tctgccaatg tattccaact
     6601 caccaaatat ccagttgttt ttatattcac ctaaaggcaa aattccacct ttgcggattg
     6661 attttttatt gtcgggaatg atggaagcta aatctgcttc tggtattgtg ccaagtccgt
     6721 tgcaacgtgg acaggcaccg taaggagagt taaaagaaaa tgtgtttggt gcaggttcat
     6781 cataagagat tcctgaagta ggacacatca gattacgact gaaaaagcga actctttttt
     6841 gatcatcagc aataatcatc aatgtattct tgccaaaacg catggcagtt tttacggtgt
     6901 cataaagtct ttttgatgcc gtttcgttaa tttcaatttt gtccactaca atttcaatgt
     6961 catgagtttt gtagcggtcg gtttgcatac ctttttttat ctcagtaatc acaccatcaa
     7021 ttcgcacacg gataaaacct gatactctta tttgttcaaa cagttcgcgg taatgtcctt
     7081 tacgtgcttt gacaacagga gcaagcacaa caatattttt cttgtcaaaa tcacgaagaa
     7141 ttatttctac aatctgatca tcactcagtc tcaccatttt ttcacccgtc atgtaggaga
     7201 aagcttcacc ggcacgtgca aaaagcaaac gcatgaaatc ataaatttca gtaaccgttc
     7261 ctactgttga gcgcggattc tgattggtag ttttttggtt caatgctgat aacaggagag
     7321 aggccgttta ttttatctac atcagggcgt tccaggctgc caagaaattg tcttgcatag
     7381 gcactgaaac tttccatata gcgtctttgt ccttcagcgt aaatagtgtc gaatgccaat
     7441 gacgacttac cactgccgct gagtcctgta aagacaatca gtttttcgcg cggtaaaacg
     7501 aggtctatat ttttgagatt atgttccctt gctccgtaaa tttcaatgct ctcgtgctct
     7561 gtttgcatat agtaataacc tgcaaaatta acttattgca gcctcttatt tggatttgtt
     7621 aataaaaatg aaggcgctgt ttttcttgat gctaaatcgt cagcataaat tcattgtaag
     7681 aaaatctgac agtttagaaa aacttgttga gtgatgaacc ttgcttgaaa aaataaaata
     7741 acaacaacgg cagtccgata ttaagattta tcagatacag caactcactg ttatgcgtaa
     7801 gcttccagaa ccttgcaata tattgtgtag gaaaccattc ttcaacatga aaaaccggaa
     7861 caaaaccttc ctgctgtgta aaaccaacac gctctacatg gccggacaag acaaagtcgt
     7921 atgaaaacat gagtgcactc tttgacttct taaaaggtga acttcctacg agaagagcaa
     7981 catcttttcg taattcggga tacatttttt ttaagctatc agggataatt aactcacctt
     8041 ttttaatgat aatatcggca cattccggtt cgcaaaattg ctgtgttgct aaaatagtat
     8101 taggctcgct atcttggcga tatgctttag gctttttcaa tggcagcaac caatatccgg
     8161 taaatactac ccatattaaa accaataatt tcaaacccca aatcctcatc actgcaaaaa
     8221 tactaaaact gataccttac ctgtaacttt atttcacttc ttttattatt atttatttca
     8281 tcaagccctg agccaaatgt tttttgattg gtgtaaacag tctgtgcaaa acggaaccaa
     8341 acatctacac ctttggtaac tttatagcga agtgttacat aataccgaaa cccacggtta
     8401 tagaatgatg gaatagaata agaatagaga atgtcgtttt caaaagcata aatcctcgaa
     8461 tcgtagctgt ctgtatcaaa taatccatag cgcacagagc ctgaaatagg cgagctaagc
     8521 ggtttaaata aaatatcctg ataaatcata aatccttttt cattgggctt ttcttcataa
     8581 ttaattctga cccattcaat acgatttctg atagcaacag ctttagaaat tttgtaagta
     8641 aggttgaaac gatagttatc ctgcctttta aacacaagat aatctgcaac tgctgctgca
     8701 ctgctgtttt cttctttttg tgtgcgcctg tttcggaaat aaatttcaaa ttgccttgac
     8761 ggattccatg acacctgcca taaatattca taacctttag aaggtgcatc ggcaccaaaa
     8821 cggagccatg gaaatgtaaa acgatctaaa tacccattaa aaccccagta gcgatttggt
     8881 ctgattacaa tacctgcata agttcctttt tcattccttg tagtacttga ttcaccaaat
     8941 ccgcttgcat agagaaaatt aaagtcacga ggatagtttc tgttcactac agaaacacta
     9001 acggtggttc ctaaactgat gatggcacca ttaacataag caattgcacc attatcactt
     9061 cttgaaaatt ctccgaagaa attgatgttg cgatacaacc aactgtagtc aatgcccact
     9121 ttggtaaatt gttttccact gaagtcaaaa cgattgtaaa gttgaaacga gcgttgcaaa
     9181 ggtctgtcgt aacctgcatg aactgctgtt aatcctgctg tgaaattacg tgtattgtaa
     9241 cctgcattga caccatacat ttgttcgcga atggtatttt tgttttcaag ttcgcttttg
     9301 gtgcgatgat agcctgattc gataaacgaa gtaaaaatat cttgttgctc aagcgtggta
     9361 tctaaaatgc tattcaggtt tgcatctaat tttctgttac tataaaaagc atcggcattc
     9421 caatttcgtt taccaacaga aactgctaca cctcttttaa aactggattc gtttagagaa
     9481 gtataaggcc taatacctgt agaaaattta cgtacagcag caacatcggg cgacttaccg
     9541 aatgacaaac ctgtacataa tgttaatcct tgcccataaa ataaatcata atcaccaata
     9601 acagcacgtt ttataattcc gaaatcacga atagctaaat gtgcagagta ataatcaaaa
     9661 ccatttcttt gtgagccttt aaaaaattct tctccctgat ctttttctgc tgttaaatta
     9721 aaactgaatc tttgtttgta gcgatactga tattgtgtat aaagtttcca tgggctgccg
     9781 ggataggcag gatgaacttt gtctgttgct gtgctgtcgc gaaagccttt ttgtttttcg
     9841 agataacgtt ggccacgaac aataagtctg ctgttgccat cagtaaatat tttttttaag
     9901 gttgctttta tgtcttctgt atttccaact gtcacataag gtaaaatttc ttcaatgtct
     9961 tctaacgaaa gaaagtctat tgcctgaagt tcgtaaagcg aaataaattt tcctgaaatt
    10021 tcacggtgtt caataatagc atctgcctgc acttcgctaa acaatcccgt ctgcaacaaa
    10081 tcttcacgtg tagctttatt cagatttatt gggttggcag ctaattgctg aagttgctct
    10141 gtcagttcag tcaggtcagc agactcatct gctgtattgg caatgttttc aactttctct
    10201 tcaatcttac tgtcatcttg tggtggcacc acctgtgcaa gtgtaatgtt gcagaataat
    10261 actgaaataa aaaacagttg aattgttttc ataattaaaa agcataacgc aaagccaacc
    10321 ccggactgta acctaaaggt tcaaaataac ctgaagtaaa gtcagcataa aattgtgaaa
    10381 attttaatcc gaaaccaaat gtaagctgaa ctggttctgt actcacgcca ccacgaacat
    10441 acaatgcctt aacaggatga tattccagtg caattttata agtttctttt ctgtcaattg
    10501 atttaaaaat ttctgctgct gccagaactt tttcggaaaa ggaatagctg atacctgcct
    10561 tgatgattac aggaattcgc tcatcatcat aatcggtaag ttttacatgc agtggattaa
    10621 aaatgtgtac acctgctttt agtttgttaa actctgcaag cacaccacct tcaatggtga
    10681 aggatgattt gtttccataa ccttcgtcaa tgtgcgtgtt aagataatca acctgaacac
    10741 cggcactgaa aacaggactt agtttttttg caaaagcaag gccgatttta ttctgattaa
    10801 aaagtttgta tccataacgg tttatactca atgcaaacac acctttgtta gatgctagtg
    10861 gcaaagcaaa agccgcagat aatgtgttga gttctttcat gccaaagcgg ttttgaatag
    10921 ccaagcctgc ttcaggtttt tctaacattg acagacaagc ctgattgttg aatgcagcga
    10981 atacatcatt aaaagtaaca gcagcatcgc tcattgcaga ggcgcgtgca cctcctgatg
    11041 taagataggt ttgagcagat acactgctga aacagaaaag aagaatggcc agtattgttg
    11101 gcttcataag catgttttga cgaactcaaa catagggcaa ataaaattat aatgcaacta
    11161 aaaatatttc aatgagtaaa tttttgtcac atttgacttt attactattt gtattaagtt
    11221 tattcagtta tcggaaacag gaaaggaacc accttcataa gggagtatga aaaatttgtt
    11281 gtaatacata ttgtttttgt acatataatg gtgtaatttt gaaaatcagt tatcctttat
    11341 gaagactatt acttattgtt ttgccatcat tgttttttcc tgtaacatga catttgcaca
    11401 aaattgtgat ttgttatgtg atggtgattt tgaaaatcca actgttattt catcaccaca
    11461 ccttgtcaat tataagacaa ttgaatgttg gaaaactact gcaagtgaca gtatcttgga
    11521 gttttgggat tccggattta atggtgtgcc ttcattttcc ggtaatcaat ttttagaaat
    11581 aaatgcaaat gaggttgcca gtatttatca ggatgtttat ttaacagcag gagatgtaat
    11641 aacattgcat tttgcacatc gtgctcgtgt cggcattgat acacttgggg tttcagtttc
    11701 ttttcaaaat aatacacctg taattttagg atattatgca gacgatgatt tagtatgggg
    11761 ctattataca gaacaatata ctgtacctgc tacaggacaa tatagaatca gttttatatc
    11821 agtctctaat ttttataatg taccaacttt aggaaatttt atagatgcag ttgaggtttg
    11881 tttatcacct gttggatttg atgaagttaa tttacaagaa acatttttaa tttcttccca
    11941 aaagagcaca ttgaaaatag actttacatc tcaattaaaa ggagaaggca ttttaacagt
    12001 atttgatttg caggggaaag aagtatttag aatttcagta gataattcaa gaaagcaaat
    12061 tgccacagct ccattgaata gtggaattta tactgttcaa ttgattcagg ggagatcaat
    12121 aacagctaaa aaagttttga ttaattaacg aggtttttct gcaactataa ttacagaacc
    12181 tgcaatttct ttagcaatag gtattttttc aactacctga ccaataccac tcaccaatga
    12241 aattaaaaat gatggcacta caggatgtaa aaaatcataa ggaaaaatat taatctgatt
    12301 aaatcctttt tgttcaagca acttactaaa gttccatcgg ttgatagcag tttcatccgg
    12361 tgaatcacca agccattttt taatgaatgg aatatttttc tgaacaaaaa tctgcggatt
    12421 aatcatgtta ggctcagcaa aaaccatacg gccgcccggt tttaatactc ttaaaatttc
    12481 attcagcgat cgttcaaatt ctaagtgatg taatactgaa cttccgaata ctacatcaaa
    12541 tgtatttgca tcaaattgta gtttcatggc atctgccaac acaaactcta ctgcgggata
    12601 tttctctttt gcaacattta gtaattcagg tgaaaggtca gttgcaataa ttttagcacc
    12661 tgtacgctga taaaatttgc ctgaaaaaag tcctgttcca caacctattt ctaatactgt
    12721 gctgctttta tctgcgagtg aatatttggc tattaaatct gcacgtctgt cagctctaac
    12781 ctttcccgca ggactgtccc agttccatat tgcttctgca ccaccctcaa gtagtttttt
    12841 gccgtgttct aattcgtttg aaattctatc actcatcaat attttatttc atataggtaa
    12901 gaaggctcac tagttccaac ctgcttcact aattttaatt tatcaggata tttttgtcca
    12961 acaggcccca tcattctatg caatgtgttg atgacatagc cgtcattctt ttttggattt
    13021 cgccttaaac ttgcaattaa cacatgcgtt actttatttt cttttaacaa tgcaagaaca
    13081 gagtcagggt cagtgcttgg tactgtatat atcggataaa atgatttgcc tttggaatag
    13141 ataaatgaca taggtgcttt tcgtgaaaca acataagcag attcagggag actgtcaccg
    13201 caataacgac ttaattttaa aaagtttgtc caatcttcgg tatatccgta ataaatatca
    13261 ccacccatat tcttttttaa tatcggtaaa ttggttacag acttatcaac agttttcatc
    13321 agactactca ttattataat gatactaaca ataagaaaaa ttgaactgcg tagtgcggga
    13381 gtgttttgaa aagcatagta tagtccataa aaaataatca tcagaataaa tggcacgtaa
    13441 actaaaacca ttcttggttg atcccatgag gtttgtaaaa caaagaaggt caatcctgcc
    13501 atcaacaata catacagaaa tgtaaattgc atgattttgt ttttattctt gaaaatgaaa
    13561 tacaaaccaa tgccaacaaa aacaaatgta atcaacacta gacctgcatt ggttttggta
    13621 ttgtccggtg aaacaaatcc caaaatctga taaagccttt taccaatata aatacttgta
    13681 ttagaaaaaa gtctgtcaac aaatccgcca aaatcatcta ccccttttga tgcattgtaa
    13741 gggtctttct gaattaaaat ctgatactga ttactgaatt gattttgtga accccagata
    13801 aatgatttaa taacttcaaa aatgccacgc actacaacaa aagaaccaaa ggcataagct
    13861 aattttttaa aatcgcgatt gaggacaaaa tataatgcta ctgcagcaat tgcacctaca
    13921 gttatatttt ttgttagtga caacaaagta agaataattc caaaaaccaa atatttagca
    13981 atgggtatgt tatcttcttt agaggcacta agaataggcc gtataacaat aaatagcaat
    14041 gcttgtaaaa agcaaaagaa agcctcggag taggtttggc tggcaaagta taagaagtag
    14101 ctattaattc cggtaaaaat taaaactgca aacaatatag tgtaaggtat tagtttgcga
    14161 aatgcataat acattgcagt taaattcaac aaactaaata aaatagaaag tgatttgaga
    14221 ataggtaagt taatacccca aatgccaacc ggcaagctta aaaaaatcgg gtagagtggc
    14281 gctttagtac tgtaataact actaaaatct ttggtaagct taaatgcacc ttcaatataa
    14341 tcagagtcgt catttccttc actcatcttt atgtcgaaga gtaaaactga aaaaatcaat
    14401 gatacaagca ataatgaaaa aaatattata gtttcatttt tctgtaaaaa attctcaagt
    14461 tttattaaag tagtttctct tgatggttta ttgacagttt tggtgctact ttttttagca
    14521 acaacttctt tgtttttttt catttcaatg aaagttatta cacaaacttc tttgcatcag
    14581 caagaaactt ttcaagtccg atgtctgtca atggatgttt caataaatcg gtaatagcat
    14641 ttaatgggca tgtggctaca tcagcgccta actttgcaca gttgatgatg tgcatggcat
    14701 ggcgtatgct ggcagcaaga atctgtgtct gaaaaccata gttgtcgtaa atctgacgga
    14761 tttcactaat cagttgtaaa ccatcggtac tcacatcatc caagcggcca ataaatggcg
    14821 aaacataggt ggcacctgcc ttggctgcaa gcaatgcctg tcccgctgaa aaaactaaag
    14881 tgcaattggt ctttattcct tttgaagtaa aatagtgaat ggcttttatt ccatctttta
    14941 tcatcggtac tttaacaatt atctgctcgt gcaacttagc taattcttcg ccttcggcaa
    15001 caatgccgtc atagttggtg gcaataactt ctgcactgat gtcgcctttt acaatattac
    15061 agatgtcaac ataatgtttc atcactgcag ctttaccttt tattccttcc ttagccatca
    15121 gtgatgggtt ggttgttaca ccatcaagta cgcctaagtc atttgcttct tttatttgtg
    15181 ccagattggc tgtgtctata aaaaatttca ttttgtattt tgtttgatat aagtgttact
    15241 tatctattgc tttacacaac agactaaaaa tttcgtcaat acttccaatg ccgtttatgc
    15301 cgtaatattt tttttgcata cggtagtatt ctgctacagg tgcagtctta ttgttgtatt
    15361 cgttaattcg ctttttaatt atttcaggat tctgatcatc agggcgaccg gaatctttgc
    15421 cacgtagcaa tagtcgcttc gataattcag cttccgttac ttctaatgca agcatcattt
    15481 taattgaagt gtttttttgc tttaataaat tatcgagtgc tgctgcctgt gccgttgtgc
    15541 gagggaagcc atcaaaaata aatcctttgg cagttgggtt ggcatcaagt tttgattcta
    15601 tcattcctat aactacttca tcgggaacta atacaccctg gtccatcagt ttttttgctt
    15661 caagacccaa tttagtttgt ccggcaattt cgtttcgcaa caaatcgcct gttgataaat
    15721 gtatgaggcc atattgctga ataagttttt ctgactgtgt tcctttgcct gcacccggag
    15781 gaccaaataa tacaatgttg agcatgataa aaaataaatt aaagagggtt tggtgatgaa
    15841 tttctcattt cgttttattc gatattatga ttttagaaca taaatatcct gaagatttcg
    15901 tccgtagcca tcatagtcta agccatagcc tacaacaaat gcaggtggaa tttcaaaccc
    15961 tgcatagtca actgtaactt ttttctttaa tgcctgtggt ttaaaaagta atgttgccaa
    16021 tttaaccgat gccggttgat gttttgctat ttcctgcatc agatatgaaa ctgtctcgcc
    16081 tgtatcaact atatcttcaa caataataac atgcctgttg tttatattcc ctgaaagacc
    16141 aactacattt ttcatctgcc cggtagattc tgtgccgcta tatgatgata ctctgataaa
    16201 agaaatttcg catggagtct caatgttacg gaatagatcg gccataaaaa gaaaggcacc
    16261 gttaagcaca ccaataaata cagggttttt gtctttgtat tcagcattca ggtcgcgtgc
    16321 aatttccaga attctggttt cgatttgctt tcgcggaatc atgattgtga atgttttatc
    16381 aagtatggta acttcgctca ttgccacttt tttgggtgcg gctaaagtaa aaataaattc
    16441 cctatgactg acggggtttc tattcttagc ttaatttgtt ttcattgcca caggttcttt
    16501 atttttgttt aataaatagc gttaattatg aatgcgttca atcccaatat agttataggt
    16561 gttttaggtg gtggccagct tggcagaatg ttattgcaaa aagcggcaga ttacaatctt
    16621 tctattcata tacttgatcc tgatgaggct gcaccttgcc gctatatgtg taaaacattt
    16681 gagcatggcg attttcgcga ttatcaggct gtcatggcct ttggtaatga ttgtgatatt
    16741 attaccattg aaattgaaca tgtaaacata gaagcattat acgaactgga aaagttggga
    16801 aaaaaggtgt atcctcaacc acatattctt gaaatggttc aggataaagg acttcagaaa
    16861 gaattttatc agaagaataa tttacctaca gcgccatttt atttagtaga aaaaccggaa
    16921 cagataaagc cggagtttcc atttgttcaa aaactgcgca aaggtggtta tgatggtaaa
    16981 ggtgttttgg ttgttaaaga tgcagatggc atgagtcagt tgtttcaggc accaagtgta
    17041 attgagaata aagtagttat tgaagcagaa attgctgtaa ttacagcaag aaataattcg
    17101 ggagaaataa aatcatttcc agtagttgaa atgcagttta atgcagaagc caatttggtt
    17161 gaattacttt tttctccatc aaaaacttca gccgtcatac aacagaaagc agtttcgatt
    17221 gcagaagatt taatcagtaa actgaacatg gttgggctgc ttgctgtaga aatgtttgtt
    17281 acaaaagatg gacaagtgct gattaatgaa attgcaccac gaccacacaa tagcggacat
    17341 cataccatag aagcaaatca tacttcgcaa ttcgagcaac atttaagagc aattcttaat
    17401 ttaccacttg gtaactcatc aactattttg cctgcagcaa tgattaacct gttaggtgat
    17461 aaagattcta atggtagggc gatttattca ggtgtgcaag atgcattaaa tgcaggtgat
    17521 gtgcacattc atttatatgg taagaaaatc actaaacctt atcgtaaaat gggacatgta
    17581 actgtaacgg cagctactgt tgacgaggca attttaaatg cccaaaaaat tcaatcgcag
    17641 ttaaaagtaa tcagttgaag taaagtgtac gtaagcctat gtccttgccg gttatttttt
    17701 tgtactcttt caataattgt tgatgaatgg cagagtctgc ctcttgccct tctatcagca
    17761 gcatgtcgtt actgagttta attacagcaa gattattttt tatgaggcca ttttctttta
    17821 aggtcgtaaa catataatca acaaactttt tgtttgcagc attttctttg tttattccca
    17881 ttgagctttt tgccgccata gcttccgcta cttcatcctg atattttgac cagtcggatt
    17941 tctctacctc tttaccattt aagacaattc gttcaacatc atcaccgcta attgaaattt
    18001 taaatgcacc attatcatta ttaatattca cagtttgtgt ttcaacaatg tatgcatcat
    18061 ttgcactggc agcatgtgta actttgttat ttaatggggc aggattggtg atttctgttt
    18121 taactgaagg tgtttctgtt aagttttctt cagttgcctg aggttgttca atagcagcat
    18181 caggctgtgt ttctgttgcc tgtaatgcag gttgagttgt ttgctctgta ttttcctgag
    18241 ttggctgtgt tttttgttga ccttgattct gaactaaaaa ataaattcct cctccaacaa
    18301 cagcaattgt tgcaacagca atgacaggct ttagccattt catgtaaccc aaagtggttt
    18361 cttttccttc aacttctttg ttaaattgtt ctaaactttc atccagcgag actacaggat
    18421 ctgcatttct tgctgcatca aacaatttat tcatcttatt attttccatg gtgaaaaaag
    18481 taatttacaa ttatacaatt tcttcttcta atattttttt aagtgactcg cgtgcccggg
    18541 caattctgct tttcacagca gaaagactat ctccctgaat ctgctgtatt tctgctaacg
    18601 atagccctga gatttcaaac aacactatgg cttctttctg ttttagcggc aattgatcta
    18661 aggcattata caattctttt aactgaactt tgctatcggc accggcatga tcatcactca
    18721 cttgttcagc ttgttccggg tcaaaggctg tatagatttg ctttttttta tcctgaagct
    18781 tataaaccaa cctgcttgaa acggtaaaca aataactcaa taatgcatct ttatggcgaa
    18841 tggtttcaaa cttttcataa gctaccaaca ttgtctctgc aacaatatct tttacatcct
    18901 cccggttata aaccatagac tgcacaaagc gaaccagctt ttcatgtaat tgctgatagg
    18961 catctgtgaa ggctttctgt tttcccgaaa ttttctcctg accgctcatc catttaatgt
    19021 aatgtaagtg cgttttttct tcaaaaagtc gcactcctga taaaataatt ttaatggtct
    19081 aaaaataaga ataattttac ggccacaaat tgtgctgtat gcaggcttta tcatcaacaa
    19141 attttaaatt tcaaggacaa acggcttttt ataagggtaa ggtcagagat gtctattcta
    19201 ttgataacaa atggcttgtg atggtcgtta cagaccgtgt ttcggcattt gatgtggttt
    19261 tgccaaggcc aattccatat aaaggagcag ttttaaatca gattgcagca cattttatgc
    19321 aggcaactgc cgatttagtg cccaattgga ttgttgatac acccgatgtt caggtgtcta
    19381 taggtattcc atgtcagcct ttaagagtag aaatggttat cagaggatat ctttccggtc
    19441 atgcctggcg cgaatacaaa cagggaaaaa gaatgttatg tggcgttaaa ttacccgatg
    19501 gtttgaatga aagtgatgcc ttgcctgagc cgataattac gcctactact aaagctgcgc
    19561 atggtcacga tcaggatata agcaaggaag aaataattaa acagggaatt gtggatgctg
    19621 caatttatga acaggcagaa tcctatacaa aacagttgtt tagaagaggg tcggagatgg
    19681 ctgcacagcg tgggttgatt ttagtagata ccaaatatga gtttggtgtt catcaaggca
    19741 aggtttatct tattgatgaa attcacacgc ccgattcgtc acgatatttt tatgcagatg
    19801 gttatgcaga aaggcaaaaa agaaagcaac ctcaaaagca attgtcaaaa gaatttttga
    19861 gacaatggct tattgaaaat aattttcagg gattagcggg acaaacagtt ccagaaatgt
    19921 ctgatgaaat tgtacagatg gtatcggatc gatatattga attgtttgaa aatattacag
    19981 ggaaaacttt tgtaaaaccg tcaaacgaaa atatagaaca acgcatttac ggtaatatag
    20041 taaaatcatt agagaaactt acatgaggtt taattgactt aatttgaaag agtaaaataa
    20101 actgagcaat atgaacacag atgaatgaat aagaagactg atgcttaaat taagaatatt
    20161 taacattcaa atttcatcaa gaacgtttta cctttgttta aaataataaa ttatgaaaaa
    20221 acaaattgca tcgttacttt ttattgcagc cgttgtaagt acagcttctg cacagaaaat
    20281 taaaatcctt gacggtagtt tagcaggatt aaaaggagaa actaaactga atgttgtcta
    20341 tgattatagt aatatgggcg taggctcttt tgcaaaggaa gaagagtacg ttgctaaaaa
    20401 aaccagagag tataatgcaa aagaacccgg acgtggagat agttgggcaa aaagctgggt
    20461 ggcagatcgt aaaaacagat ttgaaccaag ttttgtagag ctattcaata aaaaatctcc
    20521 aatgttaata ggtgattttc ctgatgcaaa atatacaatg aaggttagca caacttttac
    20581 agagcccggt tttaatgttt atgttaccag aaaaaatgca tccattaatc ttgaaattac
    20641 tgtgtatgaa acagccaatc ctgataaacc aatttgtaaa atgcaaggtt tgagatttcc
    20701 cggtcgtact ttcggtggct atgattatga taccggcaca cgtatatctg agtcttatgc
    20761 taaagcagga aaagatttag ctgcatttat tggaaaaaat ttgaagtagt aaatttttag
    20821 acttttaaaa aagagaagcg gttcttttag aaccgcttct cttttttaac atcattcact
    20881 gtttcattgc aaaaactttt tcacttcctt ttcaatataa actgaagtac ttggaaaagc
    20941 aaaatcactt ttattttcag ctactatttt cattacccca aaattgactt cctgcctagt
    21001 gttttcaaag tcgctcaact cttgtgtttt cacaaaataa atgacttcaa tttctaatgc
    21061 acttgcccca aagttggtaa agaatacatt tggattttcg ttaactaaag ggtgttcatt
    21121 gagcatattc aatatttgct gaataatatt ctgaatctga tcagctgttg tagaatatac
    21181 caagcctatt acaaaacgat gccggtaaaa tgttctttcg gtttgatttt ctaattctgc
    21241 atccatcatt tttttattcg gaacaattac taaagttttt tcaatagtgc gtactttagt
    21301 ggaacggaaa ccaacttctt caatatggcc acgaagactg tcagtcttta ccaaatcacc
    21361 aaccacaaaa ggtttgtcca taaaaatcaa aaatgacccc aataggtttt ctaatgtgtc
    21421 tttagaagct aaagctatgg ctaatcctcc aattcctaaa ccggcaatga tgctggcaac
    21481 attgagtttg aaaactgcac caagaataaa aaataaacta atgattacag ttattgcttt
    21541 cattgatgat gcaaaaaacg gaacaaataa atcgtccatt tttgattcgg ttttgttggc
    21601 acgctcgcgc attaccaatc caatgtaatc tatcactctg agtattgtcc aggtgataaa
    21661 aaatacagtg acaatctgaa aaattaattt tattgtcatt cttacaccaa actcactttc
    21721 tgaagctagt ttccattcat caggaaaatg cacatacctg catgcaatgt atatgatgat
    21781 tagaaaaagt gttgctgaaa atggtttctg tgtcagctca acaaacttat ttacaccaat
    21841 ggttttgaaa tattttttca gaacattgaa gagtaaaaaa gtaagaattc ttgaaataag
    21901 attcttgaag acaagtcctg ccagaataag tcctgcaaac cataggtagt cttctatggt
    21961 gtttcctaat attctttgat gcagaacatc tataagcatt acaacagttt tttaagagct
    22021 atttcgaaag cagcagaggc aatacctttt ttatctttat gggtagcaat cacttcctgc
    22081 aatgcatttt taatggtggt ggctgcatca tcaaaaatca gtctgtcatc aataacggca
    22141 tttgcattat gcagataagc aaacactctt gccattccac agttagcaat aaaatcggga
    22201 attacactta catgtttgtc acaatacaat ccaatggggc cgaagaaaat ctctttatca
    22261 gcaaaaggta cattggcacc acaggaaatt acttcaagtt ttttgttgat gagcgattgc
    22321 atttgctcct gagtaaccaa tcgagatgca gcacccggaa taaatatttc tgcatcaagg
    22381 ttccacactt tttctgccat ttgttgagga gaaagcatgt tatctgccac cagcttgttt
    22441 ccctgacggt tgttgaataa tgtttttatt tcttcaaacg tatagccgtt ttcgtttatc
    22501 agtccgccat ctctgtcaat gattcctaca atttttacgc ccatttgtgc aagataaaat
    22561 cctgcagcgg cagcaacatt gccccatccc tgaatggcag ctcttttctc agaaattttt
    22621 ccaccccata actcatagta atattttacg gccgatgcaa caccataacc ggtaatcata
    22681 tcggcaactc tgtatttatg gctcaaatca ggcgatagcg aagcgtcttt caataccaat
    22741 gaaacgcctt gctgcaaatt gctgatacgg tttttaatat ttgtttcgcc atcgttaaag
    22801 tgaccaactg cagtaccttc ctgcggatga agtattccaa gtttattaca aattggaata
    22861 acatcatgta tttcgtcaac attcaaatcg ccaccggttc cgtaatatgt tttcagcaat
    22921 ggctttatgg ccttaaacca acgttcaaga acctgatttt ttctggggtc agcagggtcg
    22981 aagttaatac cggatttagc tcctcctatt ggtggaccgg atacagtaaa cttcacttcc
    23041 attgttttgg caagcgattc aacttctctt ttgtcgcatc ctttgcgcat tcttgttccg
    23101 ccaccggcag agccattgcg caagctattt attacaagcc acccttcagc gcctgtttca
    23161 gcatcactcc attcaaatac aatttcagga cgcttctctt caaatttctt tagtaaatcc
    23221 agcattatgt tttaaaaatt tgcacgaaaa tattaaaatt ttccgcttaa aattctaata
    23281 aaattcttct gttattgaaa cctcaccaac acttttgaag taaaatagtt tattattcta
    23341 atttttattt tcaatgacat gagaaatcaa agatttgaac aaaaaaagtc cataaaagac
    23401 cattgggaga cggacggcca tcatgcaaaa aagcaaaaga tggctaacca tcgtgaagaa
    23461 aaaaactgga agaacaaaat cctcaaagaa gaggatgatg attctgatga agattttttc
    23521 cggtatgatg aagatgaata gccttaaggc ataagatcct aaactaaagt ttgtgattta
    23581 agtcattagg taaacggcac caccaacaga atcttttttt gctcctgtta cagaggcaag
    23641 tgtgttttta ttgttgttta ttctcaaata gcctaaaaga gcaaatatca atgcttcttt
    23701 aaagtcaatt attttaacat caggaacaat aatttttaca gttgtttttt cctgaatttt
    23761 ttgaattaga aatttgttct tggctccacc gcctgtgatt aatgcagtag tatttgggtt
    23821 atttcctgca agggttaacg cttggctgat ttgataggca atgtgctgtg tgatggtgtg
    23881 cagcttatca ttcagtgtac cggttgaaga gtttattatc ggaagaaaat attcattaaa
    23941 ccactcagca cctaaagaac gtggataagg tttgcgataa aattctaatg cattaagttt
    24001 atcaaaaagt gttgtattta atttccctgc agcagcaagt tttccttctg catcatactt
    24061 tagtccaccg ctattggcaa ggtagtttaa cgccatattt acaggacaaa tgtcaaatgc
    24121 aatccggctg tttttcacct tgcatgaaat gtttgcaata cctccaaggt taagacaata
    24181 atcatacatg ccaaaaaaca attcatcacc tataggtact aaaggcgcac cttgtccgcc
    24241 tgatgcaaca tctaaacttc tgaaatcaca aacggtggtt attccagcaa ttgcagcaat
    24301 atcagcaccg tgaccaattt gtgtagtaaa accatttgca ggttgatgaa aaactgtgtg
    24361 accatgtgat gcaattaagt cgcaagaaag ttgatgtttt tgtaaaaaat ttaaagaact
    24421 ttctcctaaa aaaacaccaa atttgtgatg taactcaaaa agtttttcgc cagaaagtaa
    24481 attagcatta gctaattcat ctcttcgttt ttggtcatat tcaatagtct ctgcagcaac
    24541 gatttcataa tgtaagccat catttgcttc tgtaatattg accaatgcaa ggtcaagccc
    24601 gtctaacgat gtgcccgaca tcaatcccaa tactttcaag cttgcagcca tgacggcaaa
    24661 tgtaagatta tggcaaaata atattaagta attgtaacaa ttcaatcaac attaaaaaaa
    24721 tcaatttcaa ggttaaccaa atacctattt ttgcgtataa gaaatgatgg cttctaccgg
    24781 aaataaacag aatacagaca ccgctggcat tgattcggaa gtattgtata accgcatatt
    24841 gtttgcagtt atagcaatgt gttatttgct gttcggtatt ttttttagtg aaaaagttat
    24901 tgatgtgctg ctacccctga catggatggc acttgttcct ttagcattgc ttgcagtcag
    24961 tttttttctt cctgttgcga aaaagtattc gggagaacaa ctttccggaa tgtttttatt
    25021 acttactatt cacctgatgt tttttctttt caggaataag ttttcaggtc atcctgaatt
    25081 tttactagta actgtcattt tattttctaa cctgcatatc cgcaacgtca tttatctttt
    25141 gctgtacaat gtaattgtgc ttggttcatt agaatatatt ctcatttcgg gcacttcagg
    25201 aagttctgat ccggtaagac tattttttat tattgttatt gcaatgg
//
LOCUS       DAOOOL010000020        25188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_154_length_25188_cov_118.035770, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000020 DAOOOL010000000
VERSION     DAOOOL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25188
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154_length_25188_cov_118.035770"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="PKU82_03005"
     CDS             <1..1697
                     /locus_tag="PKU82_03005"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOZ81872.1"
                     /translation="VIVMREVVKANFSRRSGHFTTAEVYFKRAKELHNGRDSLSYYIY
                     YGLGSTYKSIGNFPEGSRNLYQAVAIGEQLKDTLKITQAYSNLSQLQAEKGDYDDARK
                     TLNKIFSILRGNTYMPSYLNAMHTLANIEGQTGNIKEAMKLDVRGIAIADSLGDQIIK
                     VMYQDNLARCYLAQDSFERARYYFKNNQQIEKPFNNPGWNADTEINLAEVETNDGNYA
                     LAEAHLDNAVKIFSENKQLINTLKAYTVYELLYQKQGKWQKALEVNQKYQEVYANSIN
                     EKSEQSYAEFNTLFETQQKEKKISEMKLQVAQADLRAKQKNVLLLILLSVVAIVLMLF
                     RNHRVKSKLESQQLLLENNLLQEKALNVAQEQRLQISRDLHDSLGAQLTFVNSVLDGI
                     KKSTTSIDESLKSKIETVSEFSENAVTELKNTLWVMNLKSINVSDLKFRIANYINSAA
                     EAKEEIRFHFNFEIADDIQIQTKSAMNIFRVIQEIVNNAIKYSHASEVSISIHQNKNE
                     LLIDVRDNGVGFDVDEMRTKSFGLNNIYNRVSDLKGTVDLVSGEDLGTQYKIKCLL"
     gene            1691..2347
                     /locus_tag="PKU82_03010"
     CDS             1691..2347
                     /locus_tag="PKU82_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ81873.1"
                     /translation="MMINVAVVDDNIYALRAIREKLSGYSDIDLVCIAQGGQEVIDFL
                     EKNSSLDVVLMDIEMPQMNGIEATAVIKQKYPSVKVIMITVFDDDEYIFNAIKAGADS
                     YILKESKSEKIYDTIIDTLNGGSVMSPSIAVKALQLLKKATTEISSVKSTEINVVLSV
                     REIEILEQLSRGMTNKAIAESLFISPFTVKRHIENIYQKLQAHNRIDLIEKAKKGKLI
                     "
     gene            2522..3187
                     /locus_tag="PKU82_03015"
     CDS             2522..3187
                     /locus_tag="PKU82_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOZ81874.1"
                     /translation="MAEHLKIIAIEKATHDVLHITTEKPENINYNAGQAADISINKPK
                     WENELRAFTFTSLPTDKQLEFTIKTYPQHNGVTNQLLSLKPGDELLLHGVFGTIGYKG
                     EGIFIAGGAGITPFIAIYKQLEKEGNVGNNKLLFANKTRADIIQEKKFKDLLGKYFIN
                     ILSEEKLEGFEYGYITEGLLRQYLDATGYIYLCGPEPMMVAVEKHLNKLNVDSARIVK
                     EVF"
     gene            3279..3584
                     /locus_tag="PKU82_03020"
     CDS             3279..3584
                     /locus_tag="PKU82_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HOZ81875.1"
                     /translation="MLSTESTLSERMQQLRRMGYTEDFNLKSRFAEGDKDFFKILPDD
                     FKIDLYYRFEGDSDPADEAIIYAISSERHHLKGILVNGYGVYSDPVANEILKKLKVN"
     gene            3592..4209
                     /locus_tag="PKU82_03025"
     CDS             3592..4209
                     /locus_tag="PKU82_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOZ81876.1"
                     /translation="MELLKLIDIAGIAVFSISGVFAAMEKKLDVFGVFIIAFITALGG
                     GTLRDVLIGQLPVSWMYNLNYGLIVLLSTLASMFFSNIIGNYQKTLLTFDSLGIGLFT
                     VVGIQKGIMLDFHPAVCIALGTITACFGGVIRDILLNNIPLIFQKEVYATACILGGVV
                     YFVLMRLQMNEMITEMISITFIVVFRLVAVRFNWQLPSIYKIQKK"
     gene            4303..4698
                     /locus_tag="PKU82_03030"
     CDS             4303..4698
                     /locus_tag="PKU82_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ81877.1"
                     /translation="MEKVKSHIGKENYKVIIESQSGNILIADEPVENGGKNLGLSPFE
                     LIAAALSACTSITVRMYADRKDWSLTDVNTDISVNWDKANNKTIIQRKIKFSGNLSDE
                     QKNRLLQIANVCPVHKVLTNSILINTEIF"
     gene            4886..5317
                     /locus_tag="PKU82_03035"
     CDS             4886..5317
                     /locus_tag="PKU82_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ81878.1"
                     /translation="MSQTHFYEVNVQWKEGRIGELSSPILDKTIECATPPEFPNGVPN
                     IWSPEHLFVAAINSCYMATYLAIASNFKVELEDFSCRTVAKLEMVDGKYLITQAELFP
                     TVKLKDINDAEKAQRIADKSKAGCLVTNSMKTEIIMTTKIS"
     gene            5425..6840
                     /locus_tag="PKU82_03040"
     CDS             5425..6840
                     /locus_tag="PKU82_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ81879.1"
                     /translation="MFFQHVYDKSLAQGSYFIGCQAKGEAIVIDAQRDIDIYLEIAKQ
                     NNLKITHITETHIHADFLCGSRELAAVTGAKMYLSDEGGKDWQYEFNHEGVSDGDVIT
                     VGNLTLKVMHTPGHTPESISFLLTDHPASDKPVMVFTGDFVFVGDIGRPDLLEKAAGI
                     KGTKEIGAKQMFQSLKKFEALPEYVQVWSAHGAGSACGKALGAVHSSTVGYEKIRNWA
                     FQYGNDEQGFVDFLLADQPEPPKYFAMMKHLNKVNRPLLVEVPKHAKMSKEEFLSAYN
                     KGITVIDTRNKFEFAEGFLPGSLNIQGNNSFSTWMGWLLNYQQQFILVAGDKTIVDLT
                     RKLMRIGMDNMLGYVTDVASLGIELEKSEVINLAKFKSTINNDNYQIIDVRGLTEFEA
                     GHVEGAEHIFVGTLVQNLKMINKKKQVVIHCQAGDRSAIAFSLLHKYGFKNVWNYAGG
                     MKEWVASNEATVSERQMACAI"
     gene            6878..7195
                     /locus_tag="PKU82_03045"
     CDS             6878..7195
                     /locus_tag="PKU82_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ81880.1"
                     /translation="MIGLIKNMLGMENTQLTDAIKKGAFLIDVRTPAEFAGGSASGAV
                     NIPLDKIGSQLSKFKNKSNVVVFCKSGMRSMQAKNILEQNGIVNVINGGTVNDIIKIQ
                     NAK"
     gene            7185..7811
                     /locus_tag="PKU82_03050"
     CDS             7185..7811
                     /locus_tag="PKU82_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ81881.1"
                     /translation="MQNSNSAPVNKDSQVNDPSKQSFWNERWETKQTGWDIGYPSPAI
                     AEYFSKFSDKEVAILIPGCGNAYEAEFLVSEGFKNITLVDIAPKAVEILRKKFAGNSN
                     IRTLCADFFTIEEKFDILIEQTFFCAIPPDKRKEYVQKASEVLNPEGKIVGLLFDIMF
                     EKEGPPFGGSRVEYEQLFNPLFDLKMIPCLNSITPRAGTELFIELQKK"
     gene            7907..8134
                     /locus_tag="PKU82_03055"
     CDS             7907..8134
                     /locus_tag="PKU82_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81882.1"
                     /translation="MNKVFSGWNWMRVIRLALGVFIVIQGVHDKSLVFATLGVLFAMM
                     AVFNAGCCYAGSCQMPDDNADKSQIENSEKI"
     gene            8138..8704
                     /locus_tag="PKU82_03060"
     CDS             8138..8704
                     /locus_tag="PKU82_03060"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOZ81883.1"
                     /translation="MQKGIIVLLLLTVVFGSCQIGQKQKLTEQQEKEYIIKGDSISDF
                     MQGILLKHVANAINHGGTEFAIGFCNMNAIPITDSVSALYHVQIQRLSDKNRNPDNAI
                     KTLEDSLAWQKIKSSSSSFIEQQSSGDIVYYKPIKTGMPTCLKCHGSSAEIESKTLAL
                     INEKYPDDKAVGYSQGDLRGMWKIKFSE"
     gene            8712..8903
                     /locus_tag="PKU82_03065"
     CDS             8712..8903
                     /locus_tag="PKU82_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOZ81884.1"
                     /translation="MKEFLRANWLIIAGTLAGAVGGYLYWKLVGCSSGTCAITSNPLN
                     STFYGALMGGLFLSLFQKS"
     gene            9070..9303
                     /locus_tag="PKU82_03070"
     CDS             9070..9303
                     /locus_tag="PKU82_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOZ81885.1"
                     /translation="MSKSVFYHAGCPVCVSAEHDIVNLIGANNVEVVHIGENRGRIAE
                     AEGKGVKSVPALVTPAGHVLHINFGASMADVKG"
     gene            9444..9707
                     /locus_tag="PKU82_03075"
     CDS             9444..9707
                     /locus_tag="PKU82_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HOZ81886.1"
                     /translation="MKVAVWDTYVTKKDGSVMHFDIIAPSEMKDTAVIYNYGKTYLKS
                     RGQEGQPLSAKECRFCHIESVRPDWEASIHKDGYFIYEMENCK"
     gene            9741..10352
                     /locus_tag="PKU82_03080"
     CDS             9741..10352
                     /locus_tag="PKU82_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOZ81887.1"
                     /translation="MSKSDFDLNFQNKSVDGRIVAALERISQAFRVLLWQESKDFSLS
                     PIQIQVIIFLLHHSDDKCKISYLADEFNMTKATISETVKTLEHKKLIQKVYEEFDNRS
                     YTIQLTKSGNAIATQTALFSKEFHKPIAKLSNDGKENMLLSLLNIINHLNKTGVITIQ
                     RMCLSCTHHALTTNGENHFCKLLNRELLSSELRIDCPEHVLST"
     gene            10474..10884
                     /gene="rnk"
                     /locus_tag="PKU82_03085"
     CDS             10474..10884
                     /gene="rnk"
                     /locus_tag="PKU82_03085"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOZ81888.1"
                     /translation="MSKLIINRLDYARIKKSISDAKQFKSISNSEAEKLQQELDAAKI
                     VEPEAIPSNVVTMNSIVKLSFLNNDKKIQFQIVYPEQANIKENKISIFSPIATALIGY
                     KVNDEVEWIVPAGLTRIRIDEIVYQPEAAGDFDL"
     gene            10899..12167
                     /locus_tag="PKU82_03090"
     CDS             10899..12167
                     /locus_tag="PKU82_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ81889.1"
                     /translation="MKVFSYLILSFIFSIHSVECKTVRALFIGNSYTDVNNLPEIVKQ
                     IALSTGDTLIYSKNAPGGYTFLMHTTNATTISLIQAGNWDYVILQEQSQLPSFPDNDV
                     ATQVYPYAAALDSMIKASSPCATTLFYMTWGRQNGDAANCPFFPPLCTYLGMDSLLQL
                     RYTIMAQDNHAAISPVAKVWRKIRSDFPTLNLYASDGSHPNNNGSFAAACSFYSVMFG
                     KNPQLASYNFTVTASDAVTIKTVAKEVAFDSLNYWFQYDALIADFNFSTVTNTVSFFN
                     TSQNATTFNWTFGDGSSSTLMNPSHTYTASGTFNITLIASDSVCGFADTVSKTITIST
                     SGLAAPESSNSINILPNPVLDVMTFTSVKTVSSFQIFTTDGRLILTSNRPIQSNEEHS
                     LNVSMLSSGIYFLKVYSDELNASCIKFTKF"
     gene            12264..13133
                     /locus_tag="PKU82_03095"
     CDS             12264..13133
                     /locus_tag="PKU82_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOZ81890.1"
                     /translation="MMQFIKSVFAGIGQIMLCNNIITGILFTVGIFIGNLWCGIAALL
                     AASVGTATAYLLKFSKTEIEAGLYGFSAALTGVVLIFLFENTLLIWGLVFAGAILAAL
                     LQHYFIQKCFPAYTFPFIVITWVIYFVISKFDLAQLNLQETNSFQSSTILLAGTNGFG
                     QVIFQGYFVSGILFFIAVLISSPMASCFGLAASFAGVLVALMLGYSQNDITAGIFGFN
                     AVLSAIAFAGSKKSDIAWALIVVVFTLLIHFVLISTQILSEVGGVFTFPFVAGCWITL
                     LLKYSIRNSKSIN"
     gene            complement(13203..14021)
                     /locus_tag="PKU82_03100"
     CDS             complement(13203..14021)
                     /locus_tag="PKU82_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOZ81891.1"
                     /translation="MNYIYYNLFLLFITLSNYTVSSQPNDPIPQHETFTIQSHQVGEL
                     RTINVWTPDNYKTNNDSLPVLYMPDGGIKEDFPHIANTIAKLITENKIKPLILVGIEN
                     TQRRRDLSGTTEIAKDKEIAPVVGGSEKFRAFIKDELFPEINKRYRTTSEKSIIGESL
                     AGLFIMETLFLTPEMFDNYIAFDPSLWWNNHYLVRTAKEHLSKLPPPEKRLWFAGSKA
                     KDISTYTKELAYILKKENFANIKWTYSDEPKEKHVTIFRATKEKAIIWTLNKSE"
     gene            complement(14138..14707)
                     /locus_tag="PKU82_03105"
     CDS             complement(14138..14707)
                     /locus_tag="PKU82_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOZ81892.1"
                     /translation="MAETKIKAFKNQKLWRSWLEKNQDLQEGIWLQVFKVDSGIESIK
                     ITEALDEALCFGWIDGQRKSYNEQSYLQKYTPRRAQSIWSKRNIGIVESLIKSGKMTS
                     RGFAEIEKAKADGRWARAYDSHTTMQEAEDFLKELKKHKTANAFYASLNKTNKFAINF
                     RLQTAKKPETRVKRMQEILAMLKEGKKFH"
     gene            complement(14718..15179)
                     /locus_tag="PKU82_03110"
     CDS             complement(14718..15179)
                     /locus_tag="PKU82_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOZ81893.1"
                     /translation="MSHLVDFKNVSEVGLETSPVATALAGLRANEARYFHNKYKAEFV
                     TTPVTKSKEVLKWVNTILKEERNIVIASKPLETASLMVDGIKWVFVFYESGLSINVLY
                     TLNNPKKRAVGFKLSEGMEVPAELSKFKFARQKSKLAGIIRGSFFVIKKEY"
     gene            complement(15191..16087)
                     /locus_tag="PKU82_03115"
                     /pseudo
     CDS             complement(15191..16087)
                     /locus_tag="PKU82_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
     gene            complement(16384..16719)
                     /locus_tag="PKU82_03120"
     CDS             complement(16384..16719)
                     /locus_tag="PKU82_03120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOZ81894.1"
                     /translation="MKIKQFDIWLADLNPSIGTEPGKKRPVVIVQTDLLNESHLSTLI
                     CTITTNVQKEILLLRVHLRKGQLEKLSDILVDQVRTIDNKRLIKKLGHLNKDQILKLK
                     TNLRIVLDL"
     gene            complement(16709..16939)
                     /locus_tag="PKU82_03125"
     CDS             complement(16709..16939)
                     /locus_tag="PKU82_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81895.1"
                     /translation="MKTLSLKLDDKIFNETEEITSKLKLARNRYINEAVSIYNTLNKR
                     RLLKKQLAKESKLTSKDSMDILREFEKLMDEN"
     gene            complement(17181..19535)
                     /gene="recQ"
                     /locus_tag="PKU82_03130"
     CDS             complement(17181..19535)
                     /gene="recQ"
                     /locus_tag="PKU82_03130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOZ81896.1"
                     /translation="MLAETKKKKSVKDKNEKTDTKAKVKVKEKVVEKKTKDTEKAKPV
                     AKTIVAASNKQKPTDARLKDALFKYFGFDTFKGEQEEIIQTVLRGENCFVIMPTGGGK
                     SMCYQLPALMMGGTAIVVSPLIALMKNQVDALRGFNTDEAVAHFLNSSLNKTEIAQVK
                     KDIKAGKTKLLYVAPESLTKEENVAFFKEIKVSFLAIDEAHCISEWGHDFRPEYRRLK
                     PIIEALGNIPVIALTATATEKVSLDIQKNLGMMESRLFKASFNRPNLYYEVRPKVEVL
                     KEIIKFVKNHSGKSGIIYCLSRKKVEEVASTLNVNGIKALPYHAGLDAPVRAETQDKF
                     LMEKIDVIVATIAFGMGIDKPDVRFVIHHDIPKSLEGYYQETGRAGRDGREGRCVTFY
                     SFKDIEKLEKFMKGKPVAEQEIGKQMLLETVSFAETSVCRRKYLLHYFGERYEEENCG
                     NCDNCLHPKTQIEAQDEVVLLLETVQAVKEKFAAEHIINVLIGKDDKAIKDYQHNKLE
                     VFGEGMDQDAKFWMAVVRQALLAGLLFKDIDNYGLLKLSDEGKKFMDKPHSFKITQDH
                     SYESADSDEDDEPDGGGGGAAADETLFAMLKDLCKQIAKQKNLPPFVIFQEPSLEEMA
                     IQYPINMEEMKNITGVGAGKAGKYGKPFVDLIKKYVEENEIERPQDFIVKSVVNKSGL
                     KVYLIQNIDRKISLDDMAEAKGIPLGDIITELESIVASGTKIDLNYYLNEVIDADRQQ
                     DVFDYFRTAESDSAEDALLELGENDYTIDEIRMMRVKFMSELGN"
     gene            19692..20609
                     /locus_tag="PKU82_03135"
     CDS             19692..20609
                     /locus_tag="PKU82_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOZ81897.1"
                     /translation="MESSAISGLQQYIDHDFFETVKLIFLSKGRVVCTGIGKSAIIAQ
                     KITATLNSTGTPAVFMHAADAVHGDLGVIQKDDVVICISKSGNTPEIKVLAPLLRLAG
                     NKLIAMVGDTSSYLAQQAHYILNTTVAREACPHNLAPTTSTTAQLAMGDALAVCLLEN
                     RGFSSKDFARYHPGGALGKRLYLKVGDVYKNNASPQVNPTDDLKKVIVEISGSRLGAT
                     AVVDKGKLKGIITDGDLRRMLHRSDDMLATKAKDIMTSKPKSVSPDTMAADAFILMKS
                     NNITQLVVTDKGKYLGFIHLHDLLKEGIY"
     gene            20625..21446
                     /gene="tatC"
                     /locus_tag="PKU82_03140"
     CDS             20625..21446
                     /gene="tatC"
                     /locus_tag="PKU82_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOZ81898.1"
                     /translation="MFFRRKPDPNSEMNFLDHLEALRWHLVRATLAIFVLAVVAFIYK
                     DLLFDKIIFGPKNPHFITYRLLCMLSEKWSIDLCITDIPFHLISTTMAGQFTTHMVAA
                     GIAGLILGFPYLLWEIWRFVKPALSAKEKKYTRGIVFSASFLFFCGVAFGYFILAPLS
                     INFLGSYKVSEEVQNAITLDSYISIVSIMTLACGAVFELPILVFFLTKMGIISPQFMR
                     RYRKHAFIVNLVVAALITPSPDVTSQMLVAIPLFLLYEFSIFVSVVVARQESKNE"
     gene            21458..21793
                     /locus_tag="PKU82_03145"
     CDS             21458..21793
                     /locus_tag="PKU82_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ81899.1"
                     /translation="MSKSVDEFNDYRAKMNKRILESDNLVLKRLYNLDANTYMDGALP
                     VQTKEMLGLVASMVLRCDDCIKYHLMQCKQNGITTEQVYEVFAVANIVGGTIVIPHTR
                     RAAEFWDEI"
     gene            complement(21897..22448)
                     /locus_tag="PKU82_03150"
     CDS             complement(21897..22448)
                     /locus_tag="PKU82_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ81900.1"
                     /translation="MSTKYKFQEPDAIYFVTFSVVNWIDVFTRNTYKDILLDSIRHCQ
                     KEKGLVVHAWVIMTNHVHMIISKNSLRVLPDIMRDLKKFTAYKNIGAIMESPQESRRE
                     WMFKLFKQAGENNSNNTNWQFWQQDNHPIELTDATMQEQRLNYIHENPVRAGFVYKAE
                     DYVYSSACDYGGTKGLLDLLLLE"
     gene            complement(22677..24188)
                     /locus_tag="PKU82_03155"
     CDS             complement(22677..24188)
                     /locus_tag="PKU82_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81901.1"
                     /translation="MDKKRPLLIILFLLMYAISVAQQGINNNWLMGYSSWGGQPFGQT
                     KIDFYNGTPVLSYDSLEMDFRHTHGNISDATGNMMFYTNGYYLADATNDTMQNGSGIN
                     PGAYANFASDGFAIAQAALIIQKPSSNSIYYMFHGSCDNYMMTTGCRMHQLYLTTIDM
                     NGNGGLGNVITKNVPVIIDTLNTGKIAATRHANGRDWWVMVHRVNSNQFIKLLITPTV
                     ILGPYTQNIGVTRQWDAGQAWFSQDGKKYAYYYVNGGLDIFDFDRCTATLSNAVHVTM
                     PFENGYNVGMAISPGSNALYVSNTQHVYQFDLTAANIAASQLTVATYDSFVSVFPGFP
                     SGFATLFGLSALAPDGKIYITTGNGTIHMHTIDNPDVIGLGCNVNQHSVQLQSVYFNT
                     LPNHPNYFLGCDSTLGCLCAPTVGLPTPQPPKGGVVAMTNPSNGNFTLQFNVQSTAGE
                     LEVYDVMGNLVMREYVAPWSQYKRVNIEALAKGIYLCKLKWGSVERSVKVIKE"
     gene            complement(24246..>25188)
                     /locus_tag="PKU82_03160"
     CDS             complement(24246..>25188)
                     /locus_tag="PKU82_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81902.1"
                     /translation="DETRWMFKKDLFKRIEENPVLQNDVVLNNFYIAMQGTTLAELVP
                     VEADKNELFVPDNFSEALLQQYRADAAQQLTLIEQQMVLLDTAFVNDDTATVRILSDD
                     IMDLQETVTATNTLAEDVIADETADRITTATAIGISNNILAATNTPEENEQIVNEIYL
                     RTIGSDVFEFTQDDIETLYSIAIQCPMQGGNAVFMARSLYALVDKNKQYDDFKICNLV
                     GIALRKPEKAKGDTVATVYPNPANEMATLDYSLPKDSKAEFIIRTTTNQQILNKPLRA
                     GDSKYSFSTADLKPSVYFYELRSNGELIANGKLIIIR"
BASE COUNT         7787 a         4294 c         4733 g         8374 t
ORIGIN      
        1 ctgtaattgt tatgagggag gttgttaagg caaattttag tcgcagaagc ggacatttta
       61 ccactgctga ggtttatttt aaaagagcca aggagctaca taacggaaga gattcgcttt
      121 catattatat ttattatggc ttaggttcaa catataaaag cattggaaat tttccggaag
      181 gttctcgaaa tctttatcag gctgtagcta ttggcgaaca gctaaaagac actttaaaaa
      241 ttacacaggc ttattctaac ctttcacagt tgcaagctga aaagggcgac tatgacgatg
      301 cacgaaaaac attgaataaa atattttcga ttcttagggg caatacatat atgccgtctt
      361 atctgaatgc aatgcacact ttagcaaata ttgaaggtca gacaggaaat ataaaagaag
      421 caatgaagtt ggatgtgcga ggcattgcta ttgcagacag tttaggcgac caaattatta
      481 aagtaatgta tcaggacaac ctggcacgct gttatcttgc acaggatagt tttgaaagag
      541 cacgatatta ttttaaaaat aatcaacaaa tagaaaagcc ttttaataat ccgggatgga
      601 atgccgatac agagattaac cttgcagaag ttgaaaccaa cgatgggaat tatgcccttg
      661 ctgaagcaca tttggataat gcagtaaaaa tattttcaga aaacaaacaa ttaataaata
      721 cactgaaagc ttatacagtc tatgaattgc tataccagaa acaaggtaaa tggcaaaagg
      781 cattggaagt caatcaaaag tatcaggaag tatatgcaaa ttcgattaac gaaaagagtg
      841 aacaatcgta tgctgagttt aatactcttt ttgaaacaca acagaaggaa aagaaaattt
      901 ctgaaatgaa attacaggtt gcgcaagccg atctgagagc gaaacaaaag aatgtattgt
      961 tgttaatttt gctgagtgta gtggcaatag ttttgatgtt gttcagaaat catcgagtca
     1021 agtcaaaact tgaaagccag caacttttat tggagaataa tttgttgcaa gagaaggcat
     1081 taaatgttgc acaggagcaa cggcttcaga tttcacgcga cttacatgat agcttaggag
     1141 cacaactaac ctttgtgaat tctgtattgg acggaatcaa aaaatccact acatcaattg
     1201 atgaatctct gaaatctaaa atagaaacag tgtcggaatt ttctgaaaat gcagtgacag
     1261 aacttaaaaa tacactttgg gtgatgaact taaagtcaat taatgtttct gatttgaaat
     1321 ttagaattgc aaattatata aatagtgctg ctgaggctaa agaagaaatt cgttttcatt
     1381 ttaattttga aatagctgat gacatacaaa ttcagactaa atcggcaatg aatattttca
     1441 gagtcattca ggaaatagta aacaatgcaa taaagtattc tcatgcttct gaggtttcaa
     1501 taagtattca tcagaataaa aatgaactgc ttattgatgt tcgcgataat ggtgttgggt
     1561 ttgatgttga tgaaatgaga acaaaatctt ttggattgaa taatatttat aacagagtgt
     1621 cagatctgaa aggtactgtt gatttagttt caggagagga cttaggtaca caatataaaa
     1681 tcaaatgttt attatgataa atgtggctgt tgttgatgat aatatttatg cattgcgtgc
     1741 gataagagaa aaactttcag gatattcaga tattgatctt gtttgcattg cacaaggtgg
     1801 tcaggaggtg atagattttt tagaaaagaa tagtagctta gatgttgttc ttatggatat
     1861 agaaatgcca cagatgaatg gtatcgaagc caccgctgtc attaagcaga aatatccctc
     1921 agttaaagtt ataatgataa cagtttttga tgatgacgaa tatatattta atgccattaa
     1981 agcaggagct gacagttata ttttaaaaga gtcgaaaagc gaaaagatat acgataccat
     2041 tattgatact ttaaatggag gttctgtaat gtcaccatca atcgctgtta aagcattaca
     2101 attattgaaa aaagcgacta ctgaaatatc ctctgttaaa tctacagaga ttaatgtggt
     2161 tttaagtgta agagaaatag aaattttgga acaattaagc cggggaatga ccaataaagc
     2221 cattgccgaa agtttattta tatcaccctt tactgttaaa agacatattg agaatattta
     2281 tcagaaatta caggcacaca atcgtattga cttgattgag aaagcaaaga aaggaaaact
     2341 gatatagttt tttatttttt ttgcaattaa tacgcttaaa gtattaacat caactttttg
     2401 ttgatattct gtttatagtt gcatacttat tctgaaaaca aatttgtggt tattatgtta
     2461 actcattata aaaagtcatt aaaatagaca attcaaaatg accactgaat aaaataaagt
     2521 tatggctgag catttaaaaa taattgcaat tgagaaagca acacacgatg tacttcatat
     2581 cacaacagag aaacctgaaa atattaatta taatgcaggg caggcagcag atatttcaat
     2641 caacaaaccc aaatgggaaa atgagttaag agcatttact tttacttcat tgccaacaga
     2701 caaacagtta gagtttacaa taaaaaccta tccacagcat aatggtgtaa ccaatcaact
     2761 tctctcttta aaacccggtg atgaattgct tttgcacggt gtttttggga ctattggcta
     2821 taaaggcgaa ggaattttta ttgccggtgg tgccggcatt acacccttta ttgcaattta
     2881 taagcaactt gaaaaagaag gtaacgttgg gaataacaaa ctgttgtttg ccaataaaac
     2941 acgtgctgat attattcagg aaaagaaatt caaagattta cttggcaaat atttcataaa
     3001 tatactttca gaagaaaaac ttgaaggttt tgaatatggc tatattacag aaggtttgtt
     3061 aaggcagtat ttagatgcaa caggatatat ttatctctgt gggccggaac cgatgatggt
     3121 tgctgttgaa aagcacttga ataaactgaa tgttgatagt gcacgtattg ttaaagaagt
     3181 tttttaaaaa agcgagaagg tggcgaaatg tatttgcatc aggtattgaa taagtagttt
     3241 gttttagtta tttttataat ctttaaaatt atgttatcat gttaagtacg gagtcaactt
     3301 tatcagaacg tatgcagcaa ctaagacgta tgggctacac cgaagatttt aacttaaaat
     3361 cgcgttttgc ggaaggggat aaagatttct ttaagatact tcctgatgat tttaagattg
     3421 atttgtacta tcgttttgaa ggcgattcag acccggctga tgaggcaatt atatatgcga
     3481 tttcatcaga aagacatcat ctgaaaggga ttttggtaaa tggttatggc gtgtattccg
     3541 atcctgttgc gaacgagatt cttaaaaaat taaaagtaaa ctaaatcact tttggaactg
     3601 ctgaaactaa ttgatattgc cggtatagct gtgtttagta tatcgggtgt atttgctgct
     3661 atggaaaaga aactcgatgt cttcggtgtt tttataattg cttttataac agcattaggt
     3721 ggtggtactt tgcgcgatgt attaattgga caactacctg tatcatggat gtataatttg
     3781 aattatggtt taattgtgtt attaagtacg cttgcatcaa tgttcttttc taacatcatc
     3841 ggtaattatc aaaaaacact gctgacattc gactcacttg gaataggttt gtttactgtt
     3901 gtgggaattc aaaagggaat aatgcttgat tttcatcctg ctgtctgcat tgcattggga
     3961 acaatcactg catgtttcgg tggtgttatt cgcgatattt tactcaacaa tattccatta
     4021 atttttcaaa aggaagtgta tgctactgca tgtattttag ggggagttgt ttattttgtt
     4081 ttaatgcgat tgcagatgaa tgagatgatt acagaaatga ttagtattac ttttattgtt
     4141 gttttcagac tggtggctgt aaggtttaat tggcaacttc catctattta taagattcaa
     4201 aagaagtaaa cctgaatggc tgaattttga aattgatttt ttttagagtt catttatgtt
     4261 gaatcttgtt tgaataaaaa gtaatttttg aaaacttagt ttatggaaaa agtaaaatca
     4321 catattggaa aagaaaatta taaagttata attgaatcgc aatcagggaa tattctaatt
     4381 gctgatgagc cggtagaaaa cggtggcaag aatcttgggc tttcaccatt tgagttaatt
     4441 gctgcagcat tgagcgcatg tacctcaata actgtgcgga tgtatgccga tagaaaagat
     4501 tggtctctta ctgatgtaaa tactgatatt tctgttaatt gggataaagc caataacaaa
     4561 acaattattc aacgtaagat taaattttcg ggtaatcttt cagatgagca gaagaaccga
     4621 ttgcttcaga ttgccaatgt ctgtccagtt cataaagtac ttactaattc cattctgatt
     4681 aatacagaga tattttagat taatttgtct tctgtttatc tgttttgaaa taaaacatga
     4741 tatatctcat ttaaatttta gcgaaaaaca aactacaatg taacttatgt cactgtaaaa
     4801 acagtcgggc ataggcactt ttgtgctgtc attttgaacg attagctaca ctttaaatta
     4861 ttcactaaaa ttaaaaataa cagatatgtc tcaaacacat ttttacgaag taaatgtaca
     4921 atggaaagag ggtagaattg gagagttgtc atcacccatt ctcgacaaaa caattgaatg
     4981 tgccacacca ccggagtttc ctaatggtgt tccaaatatt tggtcacccg agcatttatt
     5041 tgttgctgcc atcaacagtt gctatatggc aacttatttg gctattgctt caaactttaa
     5101 agtagaactg gaagatttca gttgtcgcac agttgccaaa ctcgaaatgg tagatggcaa
     5161 gtatttgata actcaggcag aattatttcc aacggtaaaa ctgaaagata taaatgatgc
     5221 tgaaaaagca caacgcattg ccgataagtc aaaggcaggt tgtttggtta ccaactcaat
     5281 gaagacagaa attataatga caacaaaaat cagttaatgt aacaagagta acaaagtatc
     5341 tgaaaaatca ttacttactt tgcattatag aattcagata ctttcagaat acaagcacaa
     5401 ctataacatt taaattataa ctatatgttt tttcaacacg tttacgacaa aagtttagca
     5461 caagggagct atttcatagg ctgccaggca aaaggtgaag caattgtaat tgacgcacag
     5521 cgggacattg atatttatct tgaaattgcc aaacaaaata acttaaagat tactcacatt
     5581 acagaaacac acattcatgc agacttttta tgtggctctc gtgaacttgc tgctgttaca
     5641 ggagcaaaga tgtatttatc tgacgaaggt ggcaaggact ggcaatatga attcaatcat
     5701 gaaggagtaa gtgatggaga tgtaattact gttggcaacc ttactttaaa agtgatgcac
     5761 actccgggcc atactccgga aagtattagc tttttgctga cagatcatcc ggcatcagac
     5821 aaacctgtta tggtttttac aggtgacttt gtttttgttg gggatattgg ccgtcccgat
     5881 ttattggaga aagcagcagg aataaaagga actaaagaga ttggtgccaa acaaatgttt
     5941 caatctttga aaaaatttga agcattaccc gagtatgttc aggtatggtc agcacatggt
     6001 gcaggctcgg cttgtggtaa ggctttaggt gccgttcaca gttcaactgt aggatatgaa
     6061 aaaattcgca attgggcatt tcaatatggt aacgatgagc aaggttttgt tgattttctt
     6121 ttagctgacc agcctgagcc accaaagtat tttgcaatga tgaaacatct taataaagta
     6181 aatcgtccac ttttagttga agtgcctaag catgctaaga tgtctaaaga agaatttctt
     6241 tctgcttaca acaaaggtat aaccgtaatt gataccagaa ataaatttga attcgcagaa
     6301 ggctttttac ccggaagttt aaatattcaa gggaataatt ctttctcaac ctggatggga
     6361 tggttgctta attatcagca gcaatttatt ttagttgctg gtgataaaac tattgttgac
     6421 ttaaccagaa aactcatgcg tataggaatg gataacatgc ttggttatgt aactgatgtt
     6481 gcttcattag gtattgaatt ggaaaaatca gaggtaatta atttagcaaa attcaaaagc
     6541 acaataaata acgacaacta tcaaatcatt gatgtgcgtg gtttaacaga atttgaagca
     6601 ggacatgttg aaggagctga acacattttt gttggaacct tagtgcaaaa tcttaagatg
     6661 attaataaga agaagcaggt ggttattcat tgtcaggcag gagatcgttc agcaattgct
     6721 ttttctctgc ttcataaata tggctttaag aatgtgtgga attatgccgg tggtatgaag
     6781 gagtgggtag cgagcaatga agctacagtc agtgaacgac aaatggcttg tgctatttaa
     6841 tgactttgta aatttctaac agttaaatta aaataaaatg attggactaa ttaaaaacat
     6901 gttgggtatg gaaaatacac aactaacaga tgcaattaag aaaggagcat ttcttattga
     6961 tgtgcgtact cctgcagagt ttgccggtgg tagtgcatcg ggcgcagtaa atattccgtt
     7021 ggataaaata ggaagtcagt tgtctaagtt taaaaataag tcaaatgttg tggtgttttg
     7081 caaaagtgga atgcgcagca tgcaggcaaa gaacattctt gaacaaaatg gaatagtaaa
     7141 tgttatcaat ggtggtaccg ttaatgacat tattaaaatc cagaatgcaa aatagcaact
     7201 cagcgcctgt aaataaagat tctcaggtta atgatccatc aaaacagtcc ttttggaatg
     7261 aaagatggga gactaaacaa accggctggg atataggtta cccttctcct gcaatagcag
     7321 aatattttag taaattttct gataaagagg ttgctattct tattccggga tgtggtaatg
     7381 cttatgaagc agaattttta gtgagtgaag gttttaaaaa cattaccttg gttgatattg
     7441 ctccaaaggc tgtagaaata ttaaggaaaa agtttgccgg taattcaaat atcagaactc
     7501 tttgtgctga tttttttaca atagaagaga agttcgatat tttaattgaa caaacatttt
     7561 tttgtgcaat tcctccggac aaacgtaaag aatatgttca aaaagcgtca gaagttttaa
     7621 atcctgaagg aaaaattgtt ggattattgt ttgatataat gtttgaaaaa gaaggtccgc
     7681 catttggagg aagcagagtg gaatatgagc aattgtttaa tccgttattt gatttaaaga
     7741 tgattccttg cttgaatagc attactcccc gtgcagggac agagctgttt atagaattac
     7801 aaaagaagta gttagtttat atttttaaaa ttcttatgtc atgtaagttt agtgtaagaa
     7861 agcactgaag taatatgcaa ttaaaaatta tttaatttag aatcgtatga ataaggtttt
     7921 ttcaggttgg aattggatgc gtgtaatacg tttagctttg ggagttttca ttgtcattca
     7981 gggtgtgcat gacaaaagtt tggtgtttgc aacattgggt gtattatttg cgatgatggc
     8041 agtttttaat gcaggctgtt gttatgcagg aagttgtcag atgcctgatg ataacgctga
     8101 taaatcacaa attgaaaatt cagaaaaaat ataagtaatg caaaagggaa ttattgtttt
     8161 attactacta actgttgtat tcggctcctg tcaaatcgga caaaaacaaa aacttacaga
     8221 gcaacaagag aaggagtata taatcaaagg agacagtata tctgatttta tgcaaggtat
     8281 cttgttgaag catgttgcca atgcgataaa tcatggtggt acggaatttg caatcggctt
     8341 ctgcaatatg aatgccatac caattacaga ctctgtttct gcattatatc atgttcaaat
     8401 tcagcgattg agcgataaga acagaaatcc cgataatgca attaaaacgt tggaagatag
     8461 tctggcatgg caaaaaataa aaagctcttc atctagcttt atcgaacaac aatcttccgg
     8521 agatatagtg tattacaagc ctattaaaac cggaatgccc acttgcttaa aatgtcatgg
     8581 tagtagtgca gaaattgaat caaaaacttt agcgctgata aatgaaaaat acccggatga
     8641 caaggcagta ggatatagcc aaggtgattt aagaggaatg tggaaaatta aatttagcga
     8701 ataatataag catgaaagaa tttttacgtg caaattggtt aattattgcc ggtacacttg
     8761 caggagccgt aggaggatat ttatattgga aacttgtagg ctgcagcagc ggaacttgtg
     8821 ctattacctc taatccttta aacagcacat tttacggagc tcttatggga gggctttttt
     8881 tatctctatt tcaaaagagc tgataatcag atctgtttta attgtcccgc acaccatagt
     8941 tggaattgga tgaaacctgt cttttaaatt aatatttccc caaaatagta attttatttg
     9001 taaaagttag gaatcctaac taaaattgca gcggtaataa tgccaaataa tatcataaca
     9061 tcaaacaaaa tgagtaaatc agtattttat catgcaggat gccctgtatg cgttagtgca
     9121 gagcacgaca ttgtaaattt aatcggagcc aacaatgttg aagtagttca cattggtgaa
     9181 aatcgtggcc gtattgctga ggcagaaggt aaaggtgtaa aatcagtacc tgcgttggta
     9241 acacctgcag gtcatgtatt gcacattaac tttggtgcat caatggctga tgtgaaaggt
     9301 taaaaatttg tcccacatag ttaggataac tacctttgtg ggacagtttt atctgaacta
     9361 tgaattttaa acaaacaatc attttaattt ttgccataag ttgggcaact ttggcaaatg
     9421 cacaatcaaa aaataaaaaa tttatgaaag tagcagtatg ggatacctat gtaacaaaga
     9481 aggacggaag cgtaatgcat tttgatatca ttgcaccatc agaaatgaaa gacacagcag
     9541 tgatttataa ttatggtaaa acatatctga aatcaagagg gcaggagggg caaccattat
     9601 ctgcaaagga atgtcgcttt tgccatatag agtctgttag accggattgg gaagcatcca
     9661 tacataaaga tggatatttt atttacgaaa tggaaaattg taaataatag tttaatattt
     9721 ttagcttaca aattctaaag atgagcaagt ctgactttga tctgaatttt caaaataaaa
     9781 gcgttgatgg gcgtattgtt gcagcccttg aaagaatttc acaggcattt cgagtattac
     9841 tatggcaaga gagtaaagat ttttctttaa gtccaattca gattcaggtt attatttttc
     9901 tgcttcatca cagtgatgat aaatgcaaaa taagttatct ggctgatgag tttaatatga
     9961 ccaaagctac aatcagtgag actgtaaaaa cattggaaca taagaaactt attcagaaag
    10021 tttatgaaga gtttgataac agaagttata ctattcagtt aacaaagtca ggaaatgcca
    10081 ttgccacaca aacagcttta tttagtaaag aatttcataa gccaattgca aaactcagta
    10141 atgatggtaa agaaaacatg ttgttgagtc ttctaaatat tatcaatcat ctcaacaaaa
    10201 cgggtgtcat cactatacaa cgaatgtgtt tgtcctgcac acatcatgca ttaacaacaa
    10261 atggagaaaa tcatttctgt aaattactaa atcgtgaact gctttcctca gaactgcgta
    10321 ttgactgccc ggagcatgtt ttgagcactt agttgtatta accatgtaaa atcttttaag
    10381 agcattgttt tttcatacac atctgctttt taaatatttc aatagcttgg aataccttat
    10441 ctttgcaggc taattttatt ataattattt gttatgagta agttaattat caatcgtttg
    10501 gactatgcgc gtattaaaaa gtctatcagc gatgccaaac aatttaagtc aatcagcaat
    10561 tccgaagcag aaaagttaca gcaagaatta gatgctgcta aaattgttga gcctgaagca
    10621 ataccttcta atgtggttac catgaactca attgtaaaac ttagtttttt aaacaatgat
    10681 aaaaaaattc agtttcagat agtatatccc gagcaagcta atattaagga gaataaaatt
    10741 tctatttttt ctcccatagc aactgcactc attggttata aagttaatga tgaagttgaa
    10801 tggatagttc ctgccggatt aacaagaata cgcatagatg aaatcgtgta tcagccggaa
    10861 gctgccggtg attttgatct ttaaaattac ctgaatccat gaaagtattt tcatatttaa
    10921 ttttatcttt tatattttct atacattctg ttgaatgtaa aactgtaaga gcattattta
    10981 ttggcaacag ttatactgat gtaaataact tacctgaaat tgtaaaacag atagcacttt
    11041 ctaccggtga cacactgata tatagtaaga atgcacccgg aggatatact tttctaatgc
    11101 atactaccaa tgcaaccaca attagcttaa ttcaggcagg taattgggac tatgttattc
    11161 ttcaggaaca aagtcagctg ccctcatttc ctgataatga tgtagcaacg caggtttatc
    11221 cctatgcagc agcattagac agtatgataa aagcaagtag cccttgtgca actacacttt
    11281 tttatatgac ctggggaaga caaaatggtg acgctgccaa ttgcccgttc tttccaccac
    11341 tatgtactta tttgggtatg gatagtttgt tacagttgag atataccatc atggcgcaag
    11401 ataaccatgc agccatttca ccggtagcaa aagtatggcg caaaatacga tcagattttc
    11461 caacgctaaa cctatatgca agtgatggca gtcatccaaa caataatgga tcttttgctg
    11521 cagcttgcag tttctactca gttatgttcg gaaagaatcc tcaattagcg tcatataatt
    11581 ttacagttac agcatcagat gcggtcacaa ttaaaacagt ggcaaaagaa gtagcatttg
    11641 atagcttaaa ttattggttt caatacgatg cattgattgc agactttaat ttctcaactg
    11701 ttaccaatac agttagtttt ttcaatactt cgcaaaatgc tactaccttt aattggacat
    11761 ttggtgatgg cagtagcagc acattgatga acccatcaca tacttacact gcaagtggca
    11821 cttttaatat tacattgatt gcttctgatt ctgtttgtgg ttttgcagac actgtttcaa
    11881 aaacaattac aatttcaact tccggtttag cagcacctga atcttcaaat agcattaata
    11941 ttttgcctaa ccctgttttg gatgtcatga cttttacatc agttaaaaca gtttcatcat
    12001 tccaaatttt tacaactgat ggaaggctta tattaacttc taatagacca attcaatcta
    12061 atgaggagca ttcactaaat gtttctatgc tatcgtcagg tatttatttt ttgaaggttt
    12121 acagtgatga attaaatgca tcatgtatca agtttacaaa attttagaca agaaaattac
    12181 tatagaggaa tttgcaagtt attttaataa tgaattttct ggtattggat ttaatttgta
    12241 aaatctgaat ttctaaccaa gcaatgatgc aatttattaa atcggttttt gcaggcattg
    12301 gtcagattat gctttgcaat aacatcatta ccggcatact ctttacagtt ggaattttta
    12361 ttggtaactt gtggtgtggc attgctgctt tgttagctgc aagtgttggc acagccactg
    12421 catatttatt aaaatttagc aagactgaaa tcgaagcggg gttgtatgga tttagtgcag
    12481 ctttaacagg tgttgttttg atctttttgt ttgagaacac attgcttatt tggggattgg
    12541 tatttgctgg tgcaatttta gctgcattat tacagcatta ttttattcaa aaatgttttc
    12601 ctgcttacac gtttccattt attgttatta catgggtaat ttattttgtg attagcaaat
    12661 ttgatttagc tcagttaaat ttacaagaga caaattcttt tcaatcatca acaattttac
    12721 ttgcgggcac caatggcttt gggcaggtga tttttcaggg atattttgtt tcaggaatat
    12781 tattttttat agctgtatta atttcttctc cgatggcatc atgttttgga cttgccgcat
    12841 catttgctgg tgtattagta gctctcatgt taggctattc acaaaacgat attactgcag
    12901 gaatttttgg attcaatgct gtgctttctg caatagcttt tgcaggcagt aagaagtctg
    12961 atattgcatg ggcattaatt gtagttgttt ttaccctgct gattcatttt gttttaatca
    13021 gtacacaaat actaagcgaa gtaggagggg tgtttacttt tccttttgtt gcgggctgct
    13081 ggattacatt gctattaaag tatagcatca gaaattctaa gagtataaat taattcaaca
    13141 aggtactaac aaagtatttt tacacgtaat gttttgtgtg agcaatgatg ttacatttct
    13201 aattattccg atttgtttaa tgtccagatt attgcctttt ccttagttgc tctgaaaatt
    13261 gttacgtgct tttcttttgg ctcatcagaa taagtccatt taatattggc aaagttttct
    13321 tttttcagaa tgtatgccaa ctctttagta taagtcgaaa tatcttttgc ttttgaaccg
    13381 gcaaaccaca gtcgtttttc cggtggtggt agttttgata aatgttcttt tgctgttctt
    13441 accaaatagt gattgttcca ccataaagat gggtcaaatg caatgtagtt atcaaacatt
    13501 tctggtgtca aaaaaagagt ttccataata aaaagccctg ctaaagattc gcctattatg
    13561 cttttttcag atgtagttct atagcgcttg tttatttcag ggaatagttc atcttttata
    13621 aaagccctga atttttctga accgccaaca accggtgcaa tttctttgtc ttttgcaatt
    13681 tctgtagtgc ctgataagtc tcttcttcgt tgtgtgtttt cgatgcctac aagtatcaat
    13741 ggtttaattt tattttctgt tattagctta gctatcgtat ttgcaatgtg tgggaagtct
    13801 tcctttattc ctccatcggg catatacaat acaggcaatg agtcattgtt tgttttgtag
    13861 ttatccggtg tccagacatt gatagttctt aattctccta cctgatgaga ttgaatagtg
    13921 aaagtttcgt gttgtggtat cgggtcgttt ggctgtgaag atactgtgta gtttgaaagt
    13981 gtgataaaga gtagaaataa attgtaatat atgtaattca tatctttctg ttgttactgt
    14041 tttgttttaa tgtattgata aataggtagg aattttaatc aatggttaaa tcacactcag
    14101 tgagctatgc aacttaaagt aaagaggttt aaaaaattta atgaaacttc tttccttcct
    14161 tcaacattgc caaaatttct tgcattcgtt taactcttgt ttcgggcttt ttggctgttt
    14221 gtaacctaaa gttgatagca aatttattag tcttgttcaa cgatgcataa aaggcatttg
    14281 ccgttttatg ctttttcaat tctttcaaaa aatcttctgc ctcttgcatg gtagtatgcg
    14341 aatcgtatgc tcttgcccaa cgcccatctg ccttagcttt ttctatttcg gcaaaacctc
    14401 ttgaggtcat ttttcctgat ttaatcaaac tttcaactat accaatgttg cgtttagacc
    14461 aaatactttg tgcacgcctt ggcgtatatt tctgcaggta tgattgctca ttataacttt
    14521 ttctttgtcc atcaatccaa ccaaagcaaa gtgcttcgtc taaagcctct gtaattttaa
    14581 ttgattcaat tcctgaatca actttaaata cctgaagcca tattccttct tgtaagtctt
    14641 ggtttttttc taaccagctt cgccaaagtt tttggttttt aaaggctttt atttttgttt
    14701 ctgccatcaa atttaaatta atattctttt ttaataacaa agaaagagcc tctaataata
    14761 ccagccaatt tagatttttg tcttgcgaat ttgaatttac tcaactctgc cggcacttcc
    14821 attccttcgg aaagtttgaa acctacagca cgtttctttg ggttatttag agtgtaaaga
    14881 acgtttattg acaaaccact ttcgtaaaaa acaaataccc atttaatgcc atcaaccatt
    14941 agcgatgcag tttctaaagg ttttgaagca atcacaatat ttctttcttc ttttaaaata
    15001 gtgtttaccc attttaaaac ttctttgctt ttagtaacag gtgttgttac aaattctgct
    15061 ttgtatttgt tatgaaaata acgggcctca ttggcacgca aacctgcaag ggcagttgca
    15121 acaggcgaag tttctaatcc tacttcgctt acatttttaa aatctactaa atgactcatt
    15181 gcaaatttat ttataaatgt tttgtttcag gtagtgaaac aaattcttta tcatcactcg
    15241 gtatttttgg aaaaacttca gtgttttggt ttttccagtt ttctttagcc ttttccaaaa
    15301 ttgatttatc tgagtttaca aaattccaaa acatttatcg ttcctcagca aaaggctcac
    15361 ccccgaaaag ataaagtgtg gtttctccat tggtttgaaa ttcgcataac gaagcatttt
    15421 ttgcaataag taattgtttg cttccataaa tgttattttc aatagttact gttccgtcta
    15481 agacatacat tccaacttcg ccaaataatt tacttccaac attaattttc tctgctgttt
    15541 ttgtttttat ctcaatgaaa tataacggac tgtgaacaag aacaggtgat tttatcccgt
    15601 ttatttcacc tgcaatcaat ttatatgaaa cattattttc aatccattta ggaattgctt
    15661 ctttgctgaa atgaaaaaag gtaggattag tttgctctaa atgttttggc aacccaatcc
    15721 aaatttggaa accatgcaag tatttatcgt tctttcttaa atattccgga gttctttcag
    15781 aatgaaccac accttttcct gcagtcatcc aatttacttc cccgggtttt atttctatag
    15841 catttcctat actgtctcta tggaatattg ctccatcaaa aagataagta agtgtagaaa
    15901 gcccgatatg tggatgtggg ggtacatcca gattttgatg ttcttgtaaa caagccggac
    15961 ccatgtgatc tataaaaaca aaagggccaa cctttctttt ttgacggaat ggtaatagtc
    16021 ggccaaccat aaaattgcca atatctgccg ctctttcttc tattactaaa cctacgtttg
    16081 attgcatatt acacgaataa tagttttgat aatattttgt tcatgatact atcaaaatta
    16141 aaacaattca tttgctcaaa aataaaaaga aaagtgatta agaaagataa tttgaagatg
    16201 ggtaaagttt tgaatgttag aaagtttgcg agtttagaaa atttgaaagt tgttttttcc
    16261 ttagagtatt ccgatgttga atcgttgaga cagtatttag gatagcaaca cgataaggaa
    16321 agttaataaa aatgacctgt tgctcttcac acaatgcagt gacttcgcac ttacaggttg
    16381 caactataag tcaagaacaa ttcttaagtt agttttaagt tttagtattt ggtctttgtt
    16441 cagatgacca agttttttaa ttaacctttt attgtctatt gttctaacct gatctaccaa
    16501 tatgtcactt aatttttcga gttgtccttt tcttaaatga actcttaata acagaatttc
    16561 tttttgtaca ttcgttgtaa ttgtacaaat taatgttgat agatgtgact catttaaaag
    16621 gtcagtttga acaataacca caggtctttt tttgccgggc tcggttccga tacttggatt
    16681 caggtcagct aaccaaatat caaattgttt aattttcatc cattaacttt tcaaattcac
    16741 gtaatatgtc catggagtct ttactcgtca attttgattc tttggcaagt tgttttttta
    16801 ataaacgcct tttgtttaat gtattgtaaa tactaacagc ctcattgata taacgatttc
    16861 gggccaattt taattttgaa gtaatttctt cagtttcatt aaatatttta tcgtcaagct
    16921 ttaatgataa agttttcata tctctttttt tacaaaggta tatattaaaa gtatatactg
    16981 caagttttct tgacatcacc tctgatagta gtttgtttgg aagctatagc tccgatcact
    17041 attattcatt tacctcaaaa ataaatgatt cggaaaaata taattgataa ttgtggcttg
    17101 aaatgctcac ttttataaaa ctcacaaaaa aaggctgccc ttgcgaggca gccttttgtg
    17161 aacgatagat ttggttttaa tcaatttcct aattcactca taaattttac gcgcatcatt
    17221 cttatttcgt caatggtgta gtcgttttcg ccaagttcga gcaaggcatc ttctgcactg
    17281 tcactttctg ccgtgcggaa atagtcgaac acatcttgct gacggtcggc atcaataact
    17341 tcgttgaggt aataattcaa atcaattttt gttcctgatg ccacgatgct ttctaactct
    17401 gtgatgatat cgcctaaagg gatgccttta gcttcggcca tatcatcgag agaaattttg
    17461 cggtcgatgt tttgaataag ataaaccttg agacctgatt tgttgacaac agattttaca
    17521 atgaaatctt gcggacgttc aatttcgttt tcttccacat atttttttat gaggtcaacg
    17581 aatggttttc catacttgcc tgccttacct gcacccacac cggtaatgtt tttcatttcc
    17641 tccatgttga tggggtactg tatggccatt tcttcgagcg aaggctcttg gaatattaca
    17701 aaaggaggca gatttttttg tttggctatt tgtttgcaca agtctttgag catagcaaac
    17761 agtgtttcgt ctgccgctgc acctcctccg ccaccatcgg gctcatcgtc ctcatcgctg
    17821 tcggcacttt cgtagctgtg gtcttgtgtt attttgaaac tgtggggctt gtccataaat
    17881 tttttaccct catcactcaa cttaagcaat ccatagttat caatgtcttt gaagagcagt
    17941 cctgcaagca atgcctgacg cacaacggcc atccagaatt tcgcatcctg atccattcct
    18001 tcgccaaaga cttctaactt attatgctga tagtctttta tggctttgtc atcttttccg
    18061 atgagaacat tgataatgtg ctcggcagca aatttttctt ttacagcctg aacagtttcg
    18121 agcagcaata ctacttcgtc ttgtgcttcg atttgtgttt ttggatgcag gcagttgtcg
    18181 cagttgccac agttttcttc ttcatagcgc tctccaaaat aatggagcag atattttctg
    18241 cggcagactg atgtttcggc aaaggaaacg gtttcaagca gcatttgttt tcctatttcc
    18301 tgctcggcaa cgggcttgcc tttcataaat ttttcgagct tctctatgtc tttaaaagaa
    18361 tagaaagtta cgcagcgtcc ttcgcgtcca tcgcgtccgg cacggcctgt ttcctgataa
    18421 tatccttcaa gagattttgg tatgtcgtgg tgaatgacaa aacgcacatc gggcttgtca
    18481 attcccattc cgaatgcaat ggtggcaaca atgacatcaa ttttttccat cagaaatttg
    18541 tcctgcgttt cggcacgcac aggtgcatct aatcctgcat ggtagggcag ggctttgatg
    18601 ccatttacat tgagtgtgct ggcaacttct tctacttttt ttctgctgag gcaatagatg
    18661 attcctgatt ttcctgaatg gttttttaca aatttgatga tctctttaag tacttcaact
    18721 tttggtcgta cttcataata aagattaggc ctgttgaagg atgctttgaa caacctcgac
    18781 tccatcatgc ctaagttctt ttgtatatca agagaaactt tttctgttgc tgttgcagta
    18841 agtgcaataa caggaatgtt gccgagtgct tcaatgatag gttttaatct acggtattca
    18901 ggacggaaat cgtgtcccca ttctgaaata cagtgtgctt catcaatggc taaaaaagaa
    18961 acttttatct ctttaaaaaa tgcaacattc tcttctttgg ttaatgattc gggcgcaaca
    19021 tatagcaact ttgttttgcc ggcctttata tctttcttta cctgagcaat ttctgttttg
    19081 ttgagggatg agtttaagaa gtgtgccaca gcctcgtctg tgttaaatcc tctaagtgca
    19141 tctacctgat ttttcatcaa tgcaattaat ggcgaaacta ctatggcagt gccgcccatc
    19201 atcaaagcag gcagttgata gcacatagat ttgccaccac ctgtaggcat aattacaaag
    19261 cagttttcgc cacgcaatac tgtttgaata atttcttctt gctcgccctt aaaggtgtcg
    19321 aacccaaaat atttgaaaag tgcatccttc agtcgagcat ctgtaggctt ttgcttgtta
    19381 gaggcagcaa ctatagtttt tgcaaccggt ttggcttttt cggtgtcctt agttttcttt
    19441 tcaactactt tttctttcac cttcacttta gccttggtgt cggttttttc attcttgtct
    19501 ttcacagatt ttttcttctt tgtttctgct aacattttat ttattatacc aattaaaaat
    19561 ttgagttaac taaaatagat atttttgttc aataatattg caacaaataa tttttttcaa
    19621 aattaaatca gaaatacttc agattgaaat cagttcaaca aataattaaa attgccaaaa
    19681 agacgcttca gatggagtct tcggctatta gtggcttgca acaatatatt gaccatgatt
    19741 tttttgagac cgtaaaatta atatttttat cgaaaggtag ggtagtttgt acaggaattg
    19801 gtaaaagtgc cataatagct caaaaaataa cagccacact caacagtacc ggaacaccag
    19861 ccgtgtttat gcacgctgcc gatgctgtgc atggcgacct tggggtaata cagaaggacg
    19921 atgtggtgat ttgtatttcc aaaagtggca acactccgga aataaaggtc ctggcacctt
    19981 tgctaaggtt ggctggcaat aaactaatcg ccatggttgg cgacacttct tcatatctgg
    20041 cgcaacaggc acattatata cttaacacta ccgttgcccg cgaagcttgt ccgcataact
    20101 tagctcccac aacaagcact acagcacaat tggccatggg tgatgccttg gctgtgtgtc
    20161 tgctcgaaaa cagggggttc tcaagtaaag attttgcacg ttatcatccg ggtggggcat
    20221 taggaaaaag attgtatctg aaggttggcg atgtttataa aaataatgcc agtccgcagg
    20281 taaatcccac cgatgacctt aaaaaggtga ttgtagaaat atcgggaagc cgccttgggg
    20341 caactgccgt tgtagataaa ggcaagctaa aaggaataat taccgatggt gaccttagga
    20401 ggatgttgca cagaagtgac gatatgttgg ccacaaaggc taaagacatt atgacttcaa
    20461 agcccaaatc cgttagcccc gacactatgg ctgccgatgc ttttattctg atgaagtcaa
    20521 ataatattac gcagttggtt gtgactgata aaggtaagta tcttggtttt attcaccttc
    20581 acgatttgct taaagaaggc atatattaat cttcaaataa agaaatgttt ttcagaagga
    20641 agcccgaccc caactcagaa atgaattttc tggatcacct cgaagccttg cgctggcatt
    20701 tggtgcgtgc tacattggca atatttgtgt tagccgttgt agcatttata tataaggact
    20761 tattgttcga taaaattatt ttcggaccca agaaccctca ttttattacc tacagattgc
    20821 tgtgtatgct ttcggagaaa tggtcaattg acttatgcat tactgatatt ccttttcact
    20881 tgataagtac taccatggca ggacagttta ccacacacat ggtagctgcg ggaattgcag
    20941 gacttatttt aggatttcca tatttgcttt gggagatatg gcgttttgtg aaacctgcat
    21001 tgtctgccaa agagaagaaa tatacaagag gtatagtttt ttctgcttcg tttttgtttt
    21061 tctgcggagt ggcttttggt tattttattc tggcaccatt atcaattaac tttttgggaa
    21121 gttataaagt gagtgaagag gtgcagaatg ctatcacgct cgattcatat atcagcattg
    21181 taagcatcat gacattggct tgcggagcgg tttttgaact gcctatactc gtgtttttcc
    21241 ttacaaaaat gggtatcata agcccacagt ttatgagacg ctaccgtaaa catgctttta
    21301 ttgttaacct tgttgttgct gcattaatta cgccatcacc agatgtaacc agccagatgt
    21361 tggtggctat tcctttgttt ctgttatacg agttcagcat atttgtttct gttgttgtgg
    21421 caaggcagga gagtaaaaac gaataaaaaa taatcacatg agtaaaagtg tagatgagtt
    21481 caatgactat cgtgccaaaa tgaataagcg cattcttgag tcagataatc tggttttaaa
    21541 acgcctctat aatctcgatg ccaacaccta tatggatggt gcattgccgg ttcagactaa
    21601 agaaatgctt gggcttgttg ccagcatggt gctgcgctgt gatgattgta ttaaatacca
    21661 tctaatgcaa tgcaaacaaa atggtattac tacagagcag gtttatgaag tgtttgcagt
    21721 agccaatata gtgggtggaa ccattgtgat tccacatacc agaagagcag cagaattttg
    21781 ggatgagatt taaatttctt tttttagcct gaacgaaaga taggctgctt agcggctaaa
    21841 tagttaatcc ttaacaaccc acctctggcg caagtcttcg gacttgtgcc cacctatcat
    21901 tcaagcagca gtaagtccag taaacctttg gtgccgccat aatcacatgc actgctgtaa
    21961 acatagtctt ctgctttata aacaaatccc gcccgcacag gattttcgtg aatataattt
    22021 aatctttgtt cctgcatagt tgcatccgtt aattcaattg ggtgattatc ctgttgccaa
    22081 aactgccagt tagtattgtt ggaattattt tctcctgcct gcttaaagag tttaaacatc
    22141 cattcgcgcc tgctttcttg cggactttcc attatggcgc caatattttt ataagcagta
    22201 aactttttaa ggtcgcgcat gatgtcaggt aataccctca agctgttttt agaaatgatc
    22261 atatgtacat gattggtcat gataacccat gcatgaacca ctaagccttt ttctttttga
    22321 cagtgtctaa tgctgtccaa caaaatatct ttataagtat tgcgggtaaa aacatcaatc
    22381 cagtttacga ccgaaaaagt aacaaagtaa atggcatcag gctcttgaaa tttgtacttg
    22441 gtactcatgt atgcaaaggt gcaacttcta ttgattgcaa atattatttg agcacggtaa
    22501 aagccagttt agcctgcttg ctgctttgtt acgctaaaat tactgcactg cattctttat
    22561 tgatagatca gcacaagtcc gaagacttgc gccagagggt gttttattta aaattcgttg
    22621 actcgttgta gtacgcaaag taaactcagg tctgctgtct ttttctacac taaccattat
    22681 tctttaataa cctttacact tctctcaaca cttccccatt ttaacttgca caggtaaatc
    22741 ccttttgcca atgcttctat attcactctc ttatactggc tccatggtgc cacatattcc
    22801 ctcatcacca aatttcccat cacatcataa acttccaatt ctccggcagt actctgcaca
    22861 ttaaattgca aggtaaaatt cccattgctc ggattagtca tggcaacaac tcccccttta
    22921 gggggctggg gggtaggcag ccctaccgtt ggcgcacaca agcaccctaa tgtgctgtcg
    22981 catcccaaaa aataattagg atgatttggc agcgtattaa aataaacaga ttgcagttga
    23041 acactgtgtt ggttcacatt gcatcccaaa ccaattacat cagggttatc tatcgtgtgc
    23101 atatgtatag tgccattgcc tgtggtaatg taaatttttc catccggtgc aagggcagat
    23161 aaaccgaaca atgtggcaaa accactggga aaccccggga aaaccgatac aaacgaatca
    23221 taagttgcaa ctgttaattg cgatgcagca atatttgctg ctgttaaatc aaattgatac
    23281 acatgttgtg tattagaaac atataaggca ttagaccccg gagaaattgc cataccgaca
    23341 ttatagccgt tctcaaatgg catggtaaca tgcactgcat tacttagtgt tgcggtgcat
    23401 ctgtcaaaat caaaaatatc caagcctccg ttcacataat aataagcata ttttttcccg
    23461 tcctgtgaaa accatgcttg tcctgcatcc cattgtcttg taacaccaat attttgcgtg
    23521 taggggccta aaataacagt aggtgttatc aatagcttta taaattgatt agagtttacc
    23581 cggtgaacca tcacccacca gtctctgcca ttagcatgtc tggtagcagc tatcttccct
    23641 gtattgagtg tgtcaattat tacaggaaca ttttttgtta ttacatttcc taatccgcca
    23701 ttgccattca tatcaatggt tgtcaagtaa agttggtgca tcctgcatcc tgttgtcatc
    23761 atataattat cgcaggaacc atgaaacatg taataaatgg aattactact tggtttttga
    23821 ataatcaatg cagcctgcgc tattgcaaaa ccatctgatg caaagtttgc atatgctccg
    23881 ggattgattc cgctaccgtt ttgcattgta tcattagtcg catctgctaa ataatatcca
    23941 ttggtgtaaa acatcatgtt acctgttgca tctgaaatat ttccatgagt atgcctgaaa
    24001 tccatctcca aactatcata agacaaaacg ggagtaccat tgtagaaatc aattttcgtt
    24061 tgcccaaagg gttggccacc ccaacttgaa taccccatca gccagttatt atttattcct
    24121 tgttgggcaa cagaaattgc atacattaat aggaaaagaa ttataagtag tggtcttttt
    24181 ttgtccataa tcaaaggagt taaataaagg gaggaaactg ttttcaattt cccccctgaa
    24241 taattttaac gtataattat taatttacca tttgctatta actcaccatt gcttctcaac
    24301 tcataaaaat aaactgatgg ttttaaatca gccgtgctaa aagagtattt tgaatcgccc
    24361 gctcgtaaag gcttatttaa aatttgctgg tttgtagttg ttcgtataat aaattcagcc
    24421 ttgctgtctt ttggtaagct gtaatccaaa gtagccattt catttgccgg attcggataa
    24481 acagttgcaa cagtatctcc ctttgctttt tctggttttc tcaaagcaat accaacaagg
    24541 ttgcaaattt taaaatcatc gtactgctta tttttatcaa ccaaagcata tagcgaccgt
    24601 gccataaaaa cagcattgcc gccttgcatg gggcattgta tggctatgct gtacaatgtt
    24661 tcaatatcat cctgcgtaaa ttcaaataca tcgctgccaa tggtgcgcag gtaaatttca
    24721 tttactatct gctcattttc ctccggtgta ttggtggctg ccagtatgtt attacttatt
    24781 ccaatggctg ttgctgtagt aattctgtcg gcagtttcat cggctatcac atcttcagcc
    24841 aaggtgttgg tggcagttac tgtttcctgc aaatccataa tatcatctga tagtattcgc
    24901 accgttgccg tgtcatcatt tacaaatgcc gtatccaata aaaccatttg ctgctctata
    24961 agcgttaact gctgcgctgc atccgccctg tattgctgca gcaaagcttc cgaaaaatta
    25021 tcgggcacaa acaattcatt cttatcagct tccacaggca ccaattctgc caaagttgtg
    25081 ccctgcatgg caatataaaa attgttaaga acgacatcat tttgcagcac cggattttct
    25141 tcaatccttt taaacaaatc ctttttaaac atccaccgtg tttcatcc
//
LOCUS       DAOOOL010000021        24508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_157_length_24508_cov_137.825911, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000021 DAOOOL010000000
VERSION     DAOOOL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24508
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_24508_cov_137.825911"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="PKU82_03165"
     CDS             <1..1923
                     /locus_tag="PKU82_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81903.1"
                     /translation="GKGRAATGYNSIASNFNQWWQRQLDIKELKDYKNPDGSQRAWNM
                     NAEDDPSPLYWDNPYWVSYENYETDQRNRLYGGLTFDYDIGKGLSIAVSGKTDYYNDN
                     RQERVAVGSAVATAIPAYSENSITFNENNYDMILSYKHRLGDDLDFNGFVGVNRVDQK
                     FSNKFVSTQGGLNVPNFYNINNSTSSELIYTSQVRQLRKNSLFASASFGYKSFLYLDV
                     TGRNDWSSTLPEGKNSYFYPSITGSFVFSEKLDFNWLSYGKLRAGWAQTAIDPVPYRS
                     TLTSPSMSDNFNGLPTSVIPGTFNNTDLRPEMTSGFETGLELHFLQKRISLDVAYSST
                     RSKDVIFNVQQSGASGIDFKIYNAAELTNKTVELMINVIPVKLKNGLEWGVGMNWAKT
                     NNKVVKLFTDELGNETENVQIGTAPFSATLQMRKGMEASQIVGYDFVYDDKGNKVVGA
                     DGFYLRSETVKPLGSVLPDYTGGVNTYVSFKGIRLSGLIDFQKGGKVFSLTNVWGKYS
                     GIFAETAEGDIRENGLVNEGVTETGEQNTTVIAAVDHYFQDGGYVITAADVYDASFVK
                     LREMTLSYELPQSLFKKAIRGVSVGVYGRNLAILHKNVPNIDPEAGLSSGNVQGFEGG
                     QLPTARIFGVNLNVRF"
     gene            1942..3468
                     /locus_tag="PKU82_03170"
     CDS             1942..3468
                     /locus_tag="PKU82_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOZ81904.1"
                     /translation="MIKFKINQKAIATVMVFSLLLGSCKKNFESINEDPNNPKEVPNA
                     YLLTGAMRGLMDNTIDVWWGGNVGNQLGQYWSSNQYSSESRYLYRSNVTNSYWRYFYG
                     GVQNDQLVAVGGLYELSQIVKKCKEDSIEASRDGYVPNQIAVATIIRVWTFQNMTDIW
                     GDIPYSEALQADGNKTPKYDKQEDIYNGLLSEIDDAIGMINDAESGPNGDVVYNGDMT
                     AWKKFANSLKMRLALRIVDRNAGKAQSEFEAAAAGAFTSNSDNALLQYGDFPSGNPIY
                     YNRYISGRNDYASSNTFLDSTLTPLNDPRRECFFVPATATGLWTGEVYGLSEANAAQT
                     PNSRVAQRSPLVLSASLPGIFMDYAQVEFMLAEAAERGWAVTGSAESHYDAGITASIS
                     FWTALNGAAASAGDIAAYIAQPTVNYQTAGGTWQQRIGVQKWIALFDQGIQGWVEWRR
                     LDFNKLMMPADGVIDGTGIPLRMKYPVLEQTLNSASYSAAVGAQGADEQDTRMWWDVN
                     "
     gene            3579..4964
                     /gene="asnS"
                     /locus_tag="PKU82_03175"
     CDS             3579..4964
                     /gene="asnS"
                     /locus_tag="PKU82_03175"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOZ81905.1"
                     /translation="MSHSYIKALLNTEPTGQIVKAKGWVRTKRGNKAISFIALNDGST
                     IHNIQVVAENNSFDESLLKNITTGACIAVEGKLVASQGQGQKVEMQATQIEIYGTADP
                     ETFPLQKKGHTMEFLRDIAHLRPRTNTFGAVFRIRHTMAYAINHYFHTNGFFYIHTPI
                     ITGSDAEGAGEMFRVTTLDMNKPPRNEDGSIDYSQDFFAKETNLTVSGQLEGELAAMA
                     LGKIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFFDLNDNMDLAEDMLKYLVRYALDN
                     CMDDLEFLNNMYDKELIGRLRSVIENPFKRLTYTEAINVLEPNNSKFEYKVSWGADLQ
                     KEHENFLVEHFKTPVILTDYPASIKAFYMKQNDDGKTVRAMDILFPWIGEIIGGSQRE
                     ENYDKLVSRMKAMHIPEKEMWWYLDTRKFGACPHAGFGLGFERLMLFVTGMNNIRDVI
                     PFPRTPRNAEF"
     gene            complement(4975..6198)
                     /locus_tag="PKU82_03180"
     CDS             complement(4975..6198)
                     /locus_tag="PKU82_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ81906.1"
                     /translation="MTKTRNAFVAWKHKDYRLFLIAKVCLTIAIQMQSIILSWLIYEM
                     TKDPLSLGIIGLIEAIPALSVALFGGHLADRLSRRKLLIWSCAITLSASAFLAIYVYD
                     FGKISTWPIYLTIFLIGLARGFHAPTQAAFWGQLVPKEHYVNASTWNSSLWQVAAVIG
                     PAMGGFFYGWIGVFYSSLVVCAFIIVTMICYLLIKDRHVDYHNKTESILQSLKTGVNF
                     VFKNQFIISAISLDLFAVLFGGAVALLPVFADQVLHVGAQGLGLLRAAPAVGSVVMAI
                     FLAFHPPRKDAGYKMLASVVGFGLCMIGFALSGSFLLSLFLLFLSGVFDNVSVVIRST
                     IIQSFTPDEMKGRVSAVNSIFVGSSNELGAFESGVMAKLMGLIPSVLFGGGMTLLVVL
                     ITFIKAKKLKELNLP"
     gene            complement(6257..6478)
                     /locus_tag="PKU82_03185"
     CDS             complement(6257..6478)
                     /locus_tag="PKU82_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOZ81907.1"
                     /translation="MYWTLELASHLEDAPWPATKDELIDYAIRSGAPVEVVENLQELE
                     DEGESYESIEEIWPDYPTKDDFFFNEDEY"
     gene            complement(6592..7143)
                     /locus_tag="PKU82_03190"
     CDS             complement(6592..7143)
                     /locus_tag="PKU82_03190"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOZ81908.1"
                     /translation="MKIYTKTGDSGKTSLIGGTRVSKHHLRIDAYGTVDELNSTIGLL
                     KDLAEEKDLCKEILAEIQDRLFTIGSSLAADPEKSKMKIPDLNTEDITLLEKEIDRMV
                     AALPPLKSFVLPGGHLLNSHTHVARCICRRAERLATGLSESEWVEPMVLIYLNRLSDY
                     LFMLARHVCFVRNITETPWRPRV"
     gene            7368..8948
                     /locus_tag="PKU82_03195"
     CDS             7368..8948
                     /locus_tag="PKU82_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOZ81909.1"
                     /translation="MFKKVGLVLLLLAYCRSTSAQNIEIQKLGALLQMIDYYYVDTVN
                     KPKLVDDGIRAILKDCDPHSQYIPASELKEMNEPLVGKFEGVGIQFNILDDTIMVTQT
                     IAGGPSEKLGIRPGDRIVNIDGKKVAGVKITNKDVISKLRGDKGTKVKVDMYRRGEAE
                     LLTFDITRDKIPLFSVDASYKIEPDIGYIKISRFADNTVEEFKEALAKLKSQGVKSLI
                     LDLTSNGGGYLNRAHQLADEFLSDGKRIVYTEGRSQSREDYFSTAAGGWEKGKLVVMI
                     NEYSASASEIVSGAIQDWDRGLIVGRRSFGKGLVQKPYSLPDGSAVRLTVARYYTPSG
                     RCIQKSYQEGDEEYSDDISNRFKHGELYHADSIKFNDSLKYTTNAKRVVYGGGGIMPD
                     VFVPLDTMFSSKYYSDLIRKAALSEFALTYADNNRSKLKSAYTTVENFKKNFNVDDKM
                     MNDFIAFAEKKEVKKDEEGLKRSGTMIKAQIKALIARDIFNFGAYFYIINDSDDTFNK
                     AVESIKDNTFSKMKIANN"
     gene            complement(8954..11698)
                     /locus_tag="PKU82_03200"
     CDS             complement(8954..11698)
                     /locus_tag="PKU82_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81910.1"
                     /translation="MKKLILSFFFLTIIQQAKAENNQMPISAFVIQWTSAWCGSNSGA
                     LIAQVNGGVAPYSFFWNGIPGTDTLLGVPAGIYDLLVIDAQGDSAQSSGMVNAQVEVP
                     FSVISIAMSNPGSNNGAFQVVIYPGPSANDIPQGTYNYNLYDSTALNLLATHTFSYYV
                     GGAIDPYYIFDSLPAGKYYVHGVAPFGCYTQVEFRIKELPTINPTFSVSDACNGSPTG
                     NLSINLHPYPAINNNTFNSNLISSYTGAGNAVTSKYKTIITDSVNNTVGYRAVQDSLL
                     DYKNLLPGNYTLKVFTGDTIYSQGIPNIQDSILIYSTPFTIVNDPNCSLVKGRVFGDY
                     NSNCSYGFGDILFSGTLVELNPGGYSSVTNAAGEFFIPVVNGTYTLKQYAPYQFAQVC
                     PDTTTFTINITTPGTLVDVQVADSVNVNPDLQVGISGNQPRPGFNHHYKIDVRNLTPK
                     IIPAQTLTVSYDTTLNFVNAVPAPATNSNGVLMFTGISLPPFGTASFQINTVVPSTTP
                     LGTVLNASALITTVVGETIISNNIDSISQTVAGSFDPNDKAVMPHGFDAAGYITAAQQ
                     LKYTIRFQNTGTDTAFAVMIADTLPLSLNKYSLSVLNTSHPFTYEFASGNVVKFHFDN
                     ILLPDSNSNEPASHGYIKFKIDQIPGNAAGTVIQNNAAIYFDFNSPVITNTVTNTVFD
                     CNQMATVTLSNSTICENDSIQANSALLFDMNSEWLLDGTSYSNDSSFYLTGLSAGNYS
                     LTFVASNLVCSQSIINNIIVNPIPPQPFITNIANTLQSSSVSGNQWLLSGLPIGGATA
                     TSYIPTATGFYSVMVTDVNGCTKISDSIYILITSINNIGSANICIYPNPVKDELIISG
                     LTNGENVLTVFDAVGKVVLSVITSNTKEKIKTTFTKGIYFISIKNALHQIQMLRFTKE
                     "
     gene            complement(12017..12436)
                     /locus_tag="PKU82_03205"
     CDS             complement(12017..12436)
                     /locus_tag="PKU82_03205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ81911.1"
                     /translation="MTTKNIQTIQIESKPESIHIIERLVDDLKGEHNIHEDAYGNILV
                     AVTEAVNNAIQHGNKYNPNLKVKISYEVESDTISFAITDQGKGFDYYNLPDPTAPENL
                     EKPTGRGVFLMKHLADQIIFSNNGSSVELFFKTGHVA"
     gene            complement(12442..15780)
                     /locus_tag="PKU82_03210"
     CDS             complement(12442..15780)
                     /locus_tag="PKU82_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81912.1"
                     /translation="MTGNYRLLIEKLDEFIRKYYRNQVIRGSIYATGITIGFFLFISL
                     AEYFAWMGSSLRTALFYIYVFTVLAVIIRLIVIPLFKIWKLGKTLSYEQAATIIGNHF
                     TDVKDKLLNTLQLNELSENNHNELVQAGIDQKIQNLKPIPFTGAIDFKKNRKYLPLAL
                     IPAGTLIILLFAAPNVIKESTSRLVNYNVAYKKPAPFNFILLNKNFNVIEQNDFTLNF
                     KVEGQELPDQVYVTYEGRQIKADKEDKQHFTFTFHRIIKSTSFQLMADGFYSENYELK
                     SVPNPLISAFKIDLNYPAYLQKKNETLNNTGDLLIPAGTIVKWTFNTVNTSVFKLWFN
                     DSLTPLVSDNNQYTYSKRMLSGANYSIATANNFITNKDTLEYSINVIPDQYPIIQVEQ
                     QNDSSSLQRIFFKGTVKDDYGFSKLAFHYRFVKSKNDSSAHSDKLNTEILTINKNNNQ
                     TDFYHFWDGALHGVVPGDEVEYYFEVWDNDGVNGPKSAKSQSLIFKAPTLRELAENTE
                     KSNDEIKKDLQESLKQASKIQKQITDAARKLTEKKKTDFEDRKRIEDLLKQQKELDEK
                     LDKIKNEQQQNLAKQNEFKNNESQLAEKQQQIQELFDRLQNPELKELMKQLEQMLSQL
                     DKQSVQEALDKMKLNNKDLEKQMDRTLELFKQLEVEQKLKNTLENLDKLAEDQKKLSE
                     QSMDKNSDTEKLKEQQKDLNKQFEDIKKDIAALEKKNQELEFPQELANTEQEQKDIDQ
                     EQQNSEQQLNENKKSNAGKSQKNAAEKMQNMSQKMKNQMAQSESEQSEEDMASLRYLL
                     ENLLRFSFEQEALMQALKTTDVNNPQYVKMGQRQKKLQDDLKIIEDSLFALSKRVIQI
                     QQTVNTEISNIQSNIQKSIAFLQDRQVPVARSSQQYIMTSANNLALLLSEALQQMQMQ
                     QQQQKQGNGSCKKPGKGKPGPSAEKLKMMQQQLSEQMKKMKGKLEGMQKNGGKGDKGM
                     SEELARMAAQQEAIRNELNQLNQQENKDGKNSMGNLGNLANQMEQNERDIVNKKITQE
                     TLKRQQEILTRLLEAENAQREREQDQTRKAEQAKEQFLRNPAAFEEYKKLKEKQTELL
                     NTVPPGLNTFYKNLVNNYFQGLQF"
     gene            complement(15952..17289)
                     /gene="accC"
                     /locus_tag="PKU82_03215"
     CDS             complement(15952..17289)
                     /gene="accC"
                     /locus_tag="PKU82_03215"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOZ81913.1"
                     /translation="MFKKILIANRGEIALRIIRTCKEMDIRTVAVYSTADRESLHVRF
                     ADEAVCIGPPPSKDSYLNIPNIIAAAEITNSDAIHPGYGFLSENSKFSAICAKNKIKF
                     IGATPEQIDGMGDKSNAKDTMKKAGVPTIPGSEGLLTSVEEGLKIAAEIKYPVILKAT
                     AGGGGRGMRIVWKDEEFAAAWDSARQEAGAAFGNDGMYLEKYIEEPRHIEIQIAGDQY
                     GKACHLSERDCSVQRRHQKLVEETPSPFMTKELREKMGEAAIKAALAVKYEGVGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPVTEEVINYDLIKEQIKIASGEPISGKNYYPTMHAIE
                     CRINAEDPYNNFRPSPGRITNLHVPGGHGVRVDSHVYAGYTIPSNYDSMIAKLITIAQ
                     TREEAINTMERALSEYVIEGVKTTIPFHIQLMKNEDFRKGNYTTKFLESFKLL"
     gene            complement(17364..17849)
                     /gene="accB"
                     /locus_tag="PKU82_03220"
     CDS             complement(17364..17849)
                     /gene="accB"
                     /locus_tag="PKU82_03220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOZ81914.1"
                     /translation="MNKKEIEELIRFVAKAGVSEVHLEQKDFKITIKNPTKEQIVHVA
                     PAVQPVMQMPAPAQPQQVIAAPAVAAAPAAKTAGDDSKYKTIKSPMIGTFYRTPSPDK
                     PNFVNVGDEVKEGQVICIIEAMKLFNEIESDIKGKIVKVLVEHSTPVEYDQPLFLIDP
                     S"
     gene            complement(18036..23114)
                     /locus_tag="PKU82_03225"
     CDS             complement(18036..23114)
                     /locus_tag="PKU82_03225"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81915.1"
                     /translation="MEKQIIPQKLFSALKVVFVFIALFFFKGAIAQTQTFTSSGTFTV
                     PAGVTTVQVEAWGGGGAGGGVASGIFPPTRAGGGGGGGAYAKNSSVTVIPGSTITVTV
                     GAGGAGVGGADGNNGGSSSFGGLVTVSGGLGGKHGSTSPTNGAGGNGAFGTRNGGNGA
                     AASGGNSGGGGGGAGTSGNGGNASGMTGGTGGSGGGGNGANGRSSNGDGADASALSGG
                     GAGGRNSSSQWGSRTGGDGYRGQVIVTWVCPAYNITAISAVTPICPLPGTSVISLTGS
                     VTDLPVGSYTVTYNRSLPAAAGLTTTMTVTTAGSGTFTATGLTTSGSPVITVTNLSSG
                     APGGICSSVISTNNSVSVTVNAAPSVSASNDGAVCAGQTIQLHAVASGVTFQWSGPSG
                     FNSSLQDPSISGAAAANAGIYTVTATDPLAGCSATATTNVVVNALPTATASSNSPVCV
                     GFTINLSTPSVSGASYAWTGPDGFTSSIRNPSISNAQIVNDGSYTVVVTSSAGCTNTS
                     SVTVAVNNAPIASASSNAPVCTATTLNLNASPSGATYAWSGPAGYTSSTQNPTRSNAT
                     LAMNGTYTVTVTLGGCSSTATTTVVVNQAPTGTPTVSNATVCEGSAVTLNANQGLPVS
                     YSASPNVSIPDNNVSGVNSTITIPAQTIASATQLKIGINLTHTYSGDVIAKVTSPGGV
                     ITVFDRLGVPASSFGNGQNFGGYYEFDVSAGSVLPESNSGPTTIPAGTYAPSNTNGSI
                     NTNWSGISFPMNAGGNWVLNMSDRASGDVGTIVSWTVTIMTSSGITYTWTSTPNGFNS
                     TDENPSATVTGTTTYNLAATANGCTANTTVGVSATPGPLAIASSNAPVCDGNDINFIA
                     DNLAGGQGSGNSYQWYDPNNLPFSSQQNPNIFSATSAYNGTYTVVVTNQYLCTATTTV
                     QVDVNPNPVINVSTQINVNCFGESNGSITTNTSNGTGPYSYTYDFVNFNTTGEFDLLP
                     AGSYTIYVSDANACQNTVTATITEPALLQFTKTYTDQQCPSTFGSITMNATGGTTPYM
                     YSYDNGSTYVNNNANLNLTAGSYNVMVQDAHMCATSTQQQDLILVNTPSTAALSVSSQ
                     NGTELCIGGSTTLSAVGGSLGSTADWKWYKTNCGTTPVGSGPSITVNPSSSTSYYVRA
                     EDVCGATPCADLSLAITVGVPSAAVTVPMTGMPLNACVGTTANLSINAVNNTTQYVWD
                     APIGSYFDGNPLNVSPYTTSSPNVQVTFGSPNGSFYNVGVQAANACGSTLRKIQKVRG
                     IVSVPATVLGPQTVCENTSNVAYNIAPITGASQYQWSITGDATVTGNGTNVNIDFGPT
                     WNGGTLCVAAQTSCYTSASKCISLSKSAGIPNNLSGNFTACPNTSQTYSVTPLQGAAV
                     YNWTLPTGASGSSNNNSINVTFGNGYNNIGNVCVSVTSICGVTSALKCKTVAPGIPMV
                     PPAITGITNGLCNQSVAYSTPIQTGTSYNWTAPAGANVFGQGNNSVNIQFSTMTTGQV
                     CVVASNTCGTSAPRCVAVKGAPNPPTDIIALPSSWCANTQGIELTANISNTTGSYTLD
                     WGYPSAPVATYVAGGGNSNSLTLDWGTGNGVIAITASNACGSATKTYNATTNCRESEE
                     LTAANFNMYPNPASDVVNIEFWSDKDGMQKDLKVLDLSGRTVMVQKIQTISGRQSVQI
                     SLAQFAKGVYMIELNYQRNKLVIE"
     gene            23370..24134
                     /locus_tag="PKU82_03230"
     CDS             23370..24134
                     /locus_tag="PKU82_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81916.1"
                     /translation="MKHLIWLVLIYIMSAFSVKAQDPTTLIQRTPIKSWYAMNSGEFI
                     FSAGELKISDKAVYDPFGSINTIADPDNKIRFSGFFHTSQELHYNFSNVFGFYTGLAL
                     RNVGFINTMKVGGEKLTIKQRSYSLGIPVALKLGNMQTYFLTTGIEPEIMFAYKRKLF
                     YDGDKSKKSQWFSNDVNVFNPSLFAQVNFKGGMYIRVKYYLNDFLTSNTESFSLPDKP
                     VSVSYKVDSSKLYYVSIGTSIKNRKMKKPIKTSVGI"
     gene            complement(24165..>24508)
                     /locus_tag="PKU82_03235"
     CDS             complement(24165..>24508)
                     /locus_tag="PKU82_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOZ81917.1"
                     /translation="NGLLKNETPFFNNTSEIPLSKSLFENDALVFKNLKSDLIELKSK
                     NNTHGLSVTTANFPYLGIWAAKNADFICIEPWLGIADSVNASGNILEKEGIILLNQQQ
                     SFSCAFSINVF"
BASE COUNT         7383 a         4554 c         4804 g         7767 t
ORIGIN      
        1 ggtaaaggtc gtgctgcaac aggatataac agtattgcct ctaacttcaa tcagtggtgg
       61 caacgtcagc tcgatattaa agagttgaaa gattataaaa accctgatgg ctcacagcgt
      121 gcatggaata tgaatgctga agatgatcca tctccacttt attgggataa tccatattgg
      181 gtatcttacg agaattatga aaccgatcaa cgtaaccgtt tatatggagg tttgacattt
      241 gattatgata tcggaaaagg tctttctata gcagtatccg gcaaaacaga ctactataac
      301 gacaaccgcc aggagcgtgt tgctgttgga agcgcagttg caactgcaat acctgcttat
      361 tcagaaaaca gtatcacatt caatgaaaat aactatgaca tgatattgtc atacaaacac
      421 cgtttaggtg atgatttaga ttttaatggt tttgtagggg ttaacagagt agatcagaaa
      481 ttcagcaaca agtttgtttc tactcaaggt ggtttaaatg ttccgaattt ctacaacatc
      541 aataactcaa ccagcagcga attaatctat acttctcagg ttagacaact tcgtaaaaat
      601 tctctttttg caagtgcatc tttcggatat aagagtttct tatatcttga tgtaacagga
      661 cgtaatgact ggagttctac attaccagaa ggcaagaatt cttacttcta tccatcaatt
      721 acaggaagtt ttgttttctc cgaaaaactt gatttcaatt ggttgagcta tggtaaattg
      781 cgtgcaggat gggcacagac agctattgac ccggttcctt accgttcaac tttaacttca
      841 ccaagtatgt ctgacaactt taatggtttg ccaacaagtg ttatcccagg tacatttaac
      901 aataccgatt taagaccgga aatgacttca ggttttgaaa ctggattgga actgcatttc
      961 ttacagaaac gaatctctct cgatgttgct tatagctcaa cacgttcaaa agatgtaatc
     1021 ttcaatgtac aacagtcagg tgcaagtggt attgatttta aaatctacaa tgcagccgaa
     1081 ttaaccaaca aaacagttga attgatgatt aacgttattc ctgttaaatt gaaaaacgga
     1141 ttggagtggg gcgttggtat gaactgggca aaaaccaaca acaaagttgt taaactgttt
     1201 acagatgagt taggtaatga aaccgaaaac gttcagattg gaacagcacc tttctctgca
     1261 acacttcaaa tgcgcaaagg catggaagca tcgcagattg taggttatga ttttgtttat
     1321 gatgacaaag gaaacaaagt ggttggtgca gatggttttt atttacgttc agaaacagtt
     1381 aagcctttag gctcagtatt gcctgattat accggtggtg ttaataccta tgtttcattc
     1441 aaaggtatcc gcctttcagg tttaattgac ttccaaaaag gcggtaaagt tttctctctt
     1501 accaatgtat ggggaaaata ttcaggaatt tttgctgaaa cagcagaagg tgacatccgc
     1561 gaaaacggat tggttaacga aggtgtaact gaaacaggtg agcaaaacac tacagttatt
     1621 gctgcggttg accactattt tcaggatggt ggttatgtaa ttacagcagc tgatgtatat
     1681 gatgcatctt ttgtaaaatt acgcgagatg actttatctt atgaactacc tcaatcactg
     1741 tttaagaaag ccattcgagg tgtatctgtt ggtgtatatg gtcgtaatct tgctatcctt
     1801 cataaaaacg taccaaacat tgaccctgaa gcaggtttgt caagcggaaa cgtacaagga
     1861 tttgaaggtg gtcagttacc aactgcaaga atttttggtg ttaacctgaa cgtaagattt
     1921 taactttaaa aaagatagat catgataaaa tttaagataa atcaaaaagc aattgcaacg
     1981 gtgatggtat tttcgctgtt gctgggttca tgtaaaaaga attttgaatc cataaacgaa
     2041 gatccgaata accccaaaga agtaccgaat gcttatctgt taacaggtgc aatgcgcggc
     2101 ttaatggata atacaattga cgtatggtgg ggtggcaacg taggaaatca gcttggtcag
     2161 tattggtcat ctaatcaata ttcctcagag agtcgctatc tgtaccgttc taatgttacc
     2221 aactcctatt ggagatattt ttatggtggc gttcaaaacg atcagttagt agctgttggc
     2281 ggattgtatg aattgagtca gatagttaaa aagtgtaaag aagattcaat cgaagcatcg
     2341 cgtgatggct atgttcccaa tcagattgct gtagcaacaa ttatccgtgt ttggactttc
     2401 cagaacatga ctgatatttg gggtgatatt ccatattcgg aagcgctaca agccgatggt
     2461 aataaaacac caaagtacga taagcaggaa gatatctaca atggccttct aagcgagatt
     2521 gatgatgcta tcggaatgat taacgatgct gaatcaggac caaatggcga tgttgtttac
     2581 aatggcgata tgactgcatg gaaaaagttt gctaattcct taaagatgcg tttagcttta
     2641 aggattgtag atcgtaatgc tggaaaagca cagtcagagt ttgaagctgc tgctgctgga
     2701 gcatttacct ctaacagcga caatgcattg ttgcaatatg gagatttccc aagcggtaac
     2761 ccgatttatt acaaccgtta catctccgga cgtaatgact atgcatcaag caacaccttc
     2821 cttgattcta ctttaacacc attgaacgac ccacgcagag aatgtttctt tgttcctgca
     2881 actgctaccg gactatggac aggtgaagtt tatggattgt cagaagcaaa tgctgcacaa
     2941 acaccaaact cacgtgtagc acaacgctct cctttggttt tatctgctag cttacccggt
     3001 atttttatgg attatgcaca ggtagaattt atgcttgcag aagctgctga aagaggatgg
     3061 gctgttacag gctctgcaga aagtcattat gatgcaggta ttactgcaag catttctttc
     3121 tggactgcac ttaatggtgc tgctgcttct gccggtgata ttgctgcata tatagcacaa
     3181 ccaactgtaa attatcagac tgccggaggt acttggcaac aacgtattgg tgtgcagaaa
     3241 tggatagctt tatttgatca gggtattcag ggatgggttg agtggagaag acttgacttt
     3301 aataaactga tgatgcctgc cgatggtgtt attgatggta ctggtattcc attaagaatg
     3361 aaatatcctg ttcttgaaca aacacttaac agtgcaagct acagtgctgc agttggtgct
     3421 cagggcgctg atgaacaaga cacacgcatg tggtgggatg taaattaata taatgatttg
     3481 aactacttat aagggcggag gtttactccg ccttttttgt ttatgctttc ttagcatacc
     3541 gttgtttctg ataactttgc ggcaaatatt atcgcataat gagtcacagt tatattaaag
     3601 cattattaaa tactgagcca acaggtcaga ttgtaaaagc gaaaggatgg gtgcgtacaa
     3661 aaagggggaa taaagccatt tcatttattg ccttaaatga tggcagcact attcataata
     3721 ttcaggttgt tgcagagaat aatagctttg atgaatcgtt attgaaaaac ataactacag
     3781 gtgcatgtat tgctgttgaa ggtaaacttg ttgcctcaca aggacagggg cagaaggtag
     3841 aaatgcaagc cactcaaata gaaatttatg gcactgccga ccctgagaca tttccattgc
     3901 agaagaaagg tcatactatg gaatttcttc gtgatattgc acaccttcga ccacgaacaa
     3961 acacttttgg tgctgttttc agaataagac atacaatggc ctatgccatc aatcattatt
     4021 ttcataccaa cggatttttt tacatccata caccaatcat tacaggcagc gatgctgaag
     4081 gtgcaggaga aatgttcaga gtaacaacat tggacatgaa caagccacca cgcaatgaag
     4141 acggtagcat tgactatagt caggattttt ttgcaaaaga aacaaacctg acagttagcg
     4201 gacaacttga aggtgaactc gctgcaatgg cattgggaaa aatttacact ttcgggccta
     4261 cctttcgtgc cgagaacagt aatacgccac gccacttagc agaattctgg atgatagagc
     4321 ctgaaatggc tttcttcgac ctgaatgata atatggatct ggcagaagat atgctcaaat
     4381 atttagttcg ttatgcactc gataactgta tggatgattt ggagtttctc aataatatgt
     4441 acgataagga actgattggg cgtttacgca gtgtcatcga aaatcctttt aaacgtttaa
     4501 cctataccga agcaataaat gttttggaac caaacaacag taagttcgaa tataaagtta
     4561 gttggggtgc cgacctgcag aaagaacatg aaaactttct tgtagaacat tttaaaacac
     4621 ctgttatttt aactgattat cctgcttcca tcaaagcatt ttatatgaag cagaacgatg
     4681 atggaaaaac ggtcagagca atggatattt tatttccatg gataggtgaa attattggtg
     4741 gctcacagcg cgaagaaaat tacgataagt tagtcagccg tatgaaagcc atgcacattc
     4801 ccgaaaaaga aatgtggtgg tatctcgaca ccagaaaatt tggtgcatgt ccacatgctg
     4861 gttttggttt aggctttgaa cgtttgatgc tttttgtaac cggtatgaat aacatccgcg
     4921 atgtcattcc atttccacgc acaccacgca atgcagagtt ttaatttttt ctgattaagg
     4981 taaatttaat tctttaagtt tttttgcttt tataaaggta attaatacca caagcaaggt
     5041 cattcctccg ccaaagagta cagaaggtat taagcccatg agctttgcca taacaccaga
     5101 ttcaaatgcc cccaattcat ttgatgagcc tacaaaaata ctgttcactg ccgatactct
     5161 tcctttcatt tcgtcaggtg taaaactttg aataatggtt gagcgaatca ccacacttac
     5221 attatcaaaa acaccgctca aaaacaacag aaaaagagag agtaaaaaac tgcccgaaag
     5281 tgcaaaccct atcatgcata atccaaatcc aacaacgctt gccagcattt tatagcctgc
     5341 atcttttcgg ggtggatgaa atgctaagaa tatggccatt actactgagc caacagcagg
     5401 tgcagcacgc aacaaaccaa gtccttgtgc gcctacatgc aaaacctgat cggcaaaaac
     5461 aggaagcagc gccacagcgc caccaaaaag tactgcaaac aaatcgagag agatggctga
     5521 aatgataaat tgatttttaa aaacaaagtt gactcctgtt tttaaagatt gtaaaattga
     5581 ttctgttttg ttgtgatagt caacatgcct gtctttaatc agtaaataac aaatcattgt
     5641 aacaatgata aaagcacaga ctaccaacga agaataaaat acaccaatcc aaccatagaa
     5701 aaaaccaccc attgcaggac caataactgc tgccacctgc caaaggctgc tgttccatgt
     5761 tgatgcattt acataatgtt ctttgggcac taactgaccc caaaatgctg cctgcgtagg
     5821 tgcatgaaat ccgcgtgcca atccaatgag aaaaatagta aggtaaattg gccaggtaga
     5881 aattttgcca aaatcataaa cataaattgc caggaatgct gaagcagaaa gggtgatggc
     5941 acaactccat attagaagct tgcgtctgct aagtctgtcg gccaaatgcc caccaaacaa
     6001 agcaacactc aatgcaggaa ttgcttcaat caaaccaatt attcctaaag aaagcggatc
     6061 tttggtcatc tcataaatca gccagctgag aataatactc tgcatttgaa ttgctattgt
     6121 cagacaaact ttagctatca gaaacaaacg gtaatcttta tgcttccagg ctacaaatgc
     6181 atttcttgtt tttgtcataa aaaaattaag gccaagcatt tatgctcagc cttattttca
     6241 gatatcattt ataagattaa tactcatctt cgttgaaaaa aaagtcatcc ttagttggat
     6301 aatcaggcca aatttcttca atactttcat acgattcacc ttcgtcttca agctcctgaa
     6361 gattttcaac cacttctacg ggtgctcctg aacggatggc ataatcaatc aattcgtctt
     6421 tagtagcagg ccagggggcg tcttcaagat gcgatgctaa ttccagtgtc caatacatta
     6481 caatattctt attaaaaaat taaactaatt gtgccttaat ttttaagggc tgcaaaaata
     6541 tgtaaattaa attggaaaaa acaagtgttt tttttaatag ttattaagat tctaaactct
     6601 tggtctccag ggagtttctg ttatatttcg gacaaagcat acatggcgtg ccaacataaa
     6661 aagatagtca gagagcctat ttaggtagat tagtaccatt ggttctaccc attccgactc
     6721 gctcagacca gtagccagac gctctgccct gcggcaaata caacgtgcca catgagtgtg
     6781 gctgtttaac agatgaccac cgggcaaaac aaatgacttc agtggtggca gtgcggctac
     6841 cattctgtca atttcctttt ctaacagcgt aatgtcttct gtatttaaat caggaatttt
     6901 cattttcgac ttttccggat cagcagctaa tgacgagcca atggtaaaca gtctgtcctg
     6961 tatttcagcc aatatttcct tacacaggtc tttctcttcg gcaagatcct tcagtagccc
     7021 tatggtagaa tttagctcat caacagtgcc ataggcatca atacgcagat ggtgtttgct
     7081 gactcttgta ccgccaatga gtgatgtttt gccgctgtca ccggttttgg tatatatttt
     7141 cattgattaa tcagataaat aataattttt gtacaacaaa cctattaaca ttttgttact
     7201 gaaaagaagt gacagaagtt aaaatgttaa cagctttggc tgttttcttt gatagttaaa
     7261 ttaaagtttg ttaaactatc ttacagcatg ttgcttacca tagatcgcat ggattaattt
     7321 tggtatgttt actttctgta aaaacggata tttaaagttc aattataatg tttaaaaaag
     7381 taggattagt attattactg ttagcgtatt gccgaagtac atcagcacaa aacatagaaa
     7441 ttcaaaagtt gggtgcattg cttcagatga ttgattatta ttatgtggac actgttaaca
     7501 aaccaaaatt ggttgacgat ggcatcagag ctattttaaa agattgcgac ccgcactcac
     7561 agtatattcc ggcaagtgag ttgaaagaaa tgaacgagcc attggtaggt aagtttgaag
     7621 gtgtaggcat tcaattcaat attcttgatg atactattat ggtaacacaa actatagccg
     7681 gtggtccatc agaaaaatta ggtatcagac ccggtgaccg tattgtaaat attgacggaa
     7741 aaaaagtggc tggagttaaa attaccaata aggatgtcat cagcaaactc agaggcgaca
     7801 aaggcactaa agtaaaagtg gatatgtaca gacgtggcga ggcagaactt ttaacttttg
     7861 atattacacg cgataaaatt ccattgttta gtgttgatgc atcctataaa atagaacccg
     7921 atatcggtta cattaaaata agccgctttg cagataatac cgtagaggaa tttaaggaag
     7981 cacttgccaa gttaaaatcg caaggagtaa aaagtctgat tcttgattta acaagcaacg
     8041 gtggcggata tctgaatcgt gcacatcagt tggccgatga atttttaagt gatgggaaac
     8101 gcattgttta tacagaaggc cgctcacagt ccagagaaga ttatttttct actgcagcag
     8161 gtggatggga aaaaggcaaa cttgttgtca tgataaatga atactctgca tcggcaagcg
     8221 aaattgtttc gggtgccata caagattggg acagaggttt gattgttggc cgcaggtcgt
     8281 tcggaaaagg gttggttcaa aaaccatatt cattgcccga tggtagtgcc gtaagactta
     8341 ccgttgcaag atattataca ccgtcaggac gttgcataca aaagtcatat caggaaggcg
     8401 atgaagaata cagtgacgac atcagcaacc gttttaagca tggcgaactc tatcatgccg
     8461 acagtattaa attcaatgac tcacttaaat acactaccaa tgccaagcgt gtggtttatg
     8521 gtggaggagg tattatgccc gatgtgtttg taccattaga taccatgttc agctctaaat
     8581 attattcaga tttaatccgt aaagcagcat taagtgagtt tgcattaaca tacgctgaca
     8641 ataaccgcag taaattaaaa tcggcataca caacggtaga aaattttaaa aagaatttta
     8701 atgttgacga taaaatgatg aatgacttta ttgcttttgc agaaaagaaa gaagtgaaaa
     8761 aagacgaaga aggattaaag cgttcaggca ctatgattaa agcacagata aaagcactca
     8821 ttgcccgtga tatttttaat ttcggagcct acttctacat cataaacgat agtgacgata
     8881 catttaataa agcagtagaa tccattaagg ataatacttt tagtaaaatg aaaattgcaa
     8941 ataattagat ttattattcc ttcgtgaatc tcagcatttg tatttgatgt aatgcatttt
     9001 ttatgctgat gaaataaatc ccttttgtaa aagttgtttt aattttttct tttgtattac
     9061 ttgttataac agaaagtaca actttaccta ctgcatcaaa cacagttaaa acattttcgc
     9121 catttgtaag tccagaaatt attaattcgt ctttaacagg gttcggataa atacagatgt
     9181 tagcacttcc aatgttattt attgaggtta tcaatatata tattgaatcg ctaatcttgg
     9241 tgcaaccatt gacatcagtt accattacac tataaaatcc tgtagcagtg ggtatgtaac
     9301 ttgtagcggt ggcgccacca attggtagtc ccgataacaa ccattgattg ccgctgacag
     9361 acgaactttg aagagtgttg gcaatgttgg ttataaaagg ttgtggtgga attggattaa
     9421 caataatgtt gtttatgata ctttggctac ataccaaatt ggatgcaaca aaggttaacg
     9481 aataatttcc ggcacttaaa ccagtgagat aaaatgatga atcatttgag tatgatgtac
     9541 catctaaaag ccattcactg ttcatatcaa acaataatgc agagttggct tgaatagagt
     9601 cgttttcgca aatggtggag ttgctaagtg ttactgtggc catttggttg caatcgaaaa
     9661 cagtattggt aacagtgttg gtaattacgg gcgaattaaa atcaaaataa atagctgcat
     9721 tattttgtat aactgtacca gcagcatttc cgggtatttg atctatttta aatttaatat
     9781 aaccgtggct tgccggttcg tttgaattgc tgtctggtaa taaaatatta tcgaaatgaa
     9841 attttaccac attgcctgaa gcaaattcat aggtgaaagg gtgagaagtg ttcaatacac
     9901 ttaacgaata tttgttgagt gataatggaa gcgtgtccgc aatcattact gcaaaagcag
     9961 tatcagttcc ggtgttttgg aaacggatgg tgtatttgag ttgttgtgcg gctgtaatgt
    10021 aacctgcagc atcaaaacca tgcggcatta cagctttatc attagggtca aacgaaccgg
    10081 ctactgtttg tgaaatggaa tcaatattat ttgaaatgat ggtttcacca acaactgttg
    10141 ttatcaaagc agaagcattt aatactgttc ctaatggtgt agtagatgga acaacagtat
    10201 taatctgaaa tgatgcagtg ccgaaaggag gcagtgatat acctgtaaac attaaaactc
    10261 cgttgctgtt ggttgccggt gcaggaacag catttacaaa atttaaagtg gtgtcataac
    10321 tcaccgttag tgtttgagcc ggaattattt ttggagtaag attgcgaaca tcaattttgt
    10381 aatgatgatt aaatccgggt cgaggttgat tgccactaat gccaacttgt aaatcgggat
    10441 ttacgtttac actatcggcc acttgtacat caactaatgt tccgggtgta gtaatgttaa
    10501 tagtaaatgt ggtagtatca ggacatacct gcgcaaattg gtatggtgca tattgtttaa
    10561 gtgtgtaggt gccatttact accggaataa aaaattcacc ggcagcattg gttacagaac
    10621 tataaccgcc aggattaagt tctaccaatg ttcctgaaaa taaaatatca ccgaaaccat
    10681 aagaacagtt tgagttataa tcaccaaaca cacgaccttt taccaaagaa caattaggat
    10741 cattaacaat tgtaaaaggt gtggaataaa tcaaaataga atcctgtatg ttgggtatgc
    10801 cttgtgagta gatagtgtcg cctgtaaaaa cttttaaagt gtaatttccg ggaagaagat
    10861 tcttgtaatc gagtaatgaa tcctgtacag cacgataccc tactgtgttg ttaacagaat
    10921 ctgttattat ggttttgtat ttggaagtaa ctgcatttcc agctcctgtg tagcttgaaa
    10981 ttaaattgct gttaaaagta ttgttgttaa ttgcagggta ggggtgaaga ttaatagaaa
    11041 gatttcctgt aggagaacca ttgcatgcat ccgatacaga aaaagttgga ttaatagtgg
    11101 gcagttcttt aattcggaat tccacctggg tgtagcatcc gaatggtgcc acgccatgca
    11161 cataatattt gccagcaggt aagctgtcaa aaatgtaata aggatctatg gctccgccta
    11221 cataataact aaatgtatgt gtcgcgagca aatttaaagc agtgctgtca tacaaattgt
    11281 aattataagt accttgaggt atgtcattag ccgaaggacc tggataaata actacttgaa
    11341 atgcaccgtt atttgagccc gggtttgaca tagcaattga gataacagaa aaaggcactt
    11401 caacttgggc attcaccatg ccgctgcttt gtgctgagtc gccttgtgca tcaataacca
    11461 ataaatcata aatgccggca ggaacaccca ataaagtatc agtgccggga atgccattcc
    11521 aaaagaagga gtatggtgca acaccaccgt ttacctgagc tattaacgcc cctgagttac
    11581 tgccgcacca ggcacttgtc cattgtataa caaacgcaga aatgggcatt tggttattct
    11641 cagcttttgc ctgctgaata atagtaagaa aaaagaaact taatattaat tttttcattg
    11701 aataaataat tagttgaact ataaggaatt ttttcaatta caaaattata tagtcataat
    11761 cgaataaaga atagtaggaa aaaaaattaa aaaaaattgg cttgaatata gattgtatta
    11821 attgaattag gagttaagat gaaattggca atttataatt atagataaat ccggttttcc
    11881 aattaattca aatgagttaa tttatttgaa gctattttat taggaaaaat aaacggttat
    11941 agaaaatggc atttcataag tctataaact tgcaaaacaa aaatagaatg ataaaatagc
    12001 aatattagca atgttattat gctacatgcc cggttttaaa aaacaactca acagacgagc
    12061 cattgtttga aaaaataatc tgatcggcta aatgtttcat taaaaagaca ccgcgtcctg
    12121 ttggcttttc aagattttcg ggagcagtag ggtcgggcag gttataatag tcaaatcctt
    12181 ttccctgatc ggtaatagca aaagaaatag tgtcgctttc tacttcgtag ctgattttaa
    12241 ctttcaggtt tgggttgtat ttgtttccat gctgtatggc attgtttaca gcctctgtta
    12301 cggctaccag aatgttgcca taagcatctt cgtgaatgtt gtgttcgcct ttaagatcgt
    12361 caaccaaacg ctctataata tgtatgcttt ccggtttaga ttctatctga attgtctgga
    12421 tgttttttgt tgtcatctgt cttaaaattg aagtccttga aaataattat ttaccaaatt
    12481 tttgtaaaat gtatttaaac ccggaggtac tgtattcagt agttccgttt gtttttcttt
    12541 tagtttttta tactcctcaa aagcggcagg gttacgtaaa aattgctctt ttgcttgctc
    12601 tgccttacgt gtctgatctt gttctctttc acgctgcgca ttctccgctt caagcagtcg
    12661 tgtaagtatt tcctgctgcc tttttaaggt ttcttgcgtt atttttttat tcacaatatc
    12721 gcgctcgttt tgctccatct gattggccaa attaccgagg ttgcccatag agtttttacc
    12781 atctttattt tcctgctggt tcaactggtt taactcattt ctgatagcct cctgttgggc
    12841 agccattctc gctaactctt cgctcatacc tttgtcaccc ttacccccgt ttttttgcat
    12901 tccttctaac tttcctttca tttttttcat ttgttcagaa agttgttgtt gcatcatttt
    12961 taatttttct gcagatggtc ccggtttgcc tttgcccggt ttcttacatg agccattgcc
    13021 ctgcttctgt tgttgctgct gcatttgcat ttgttgtaat gcttcactga gcagtagtgc
    13081 cagattattg gccgatgtca taatgtattg ctgcgagctg cgagccacag gaacctgcct
    13141 gtcctgcaaa aatgcaatag atttttgaat atttgactga atgttagaaa tttcagtatt
    13201 taccgtctgc tgaatctgaa ttacacgctt gctaagggca aataatgagt cttcaataat
    13261 tttcaggtcg tcctgcagtt ttttctgcct ctgacccatt tttacatact gtggattgtt
    13321 gacatctgta gtctttaatg cctgcatcag tgcttcctgc tcaaatgaaa aacgaagcag
    13381 gttctctaat aaatacctta atgatgccat gtcctcttcc gattgttccg attcactctg
    13441 tgccatttgg tttttcattt tctgcgacat gttctgcatc ttttccgcag catttttttg
    13501 cgatttaccg gcattcgact ttttgttttc gttcaactgc tgttcgctgt tctgttgctc
    13561 ctgatctatg tctttttgct cctgctctgt gtttgccagc tcctgtggaa attccagttc
    13621 ctgatttttc ttttctaatg ctgcaatatc ttttttaatg tcttcaaact gtttgttcag
    13681 atccttctgt tgttctttta atttttctgt atcggaattt ttatccatgc tttgctccga
    13741 tagttttttc tgatcttctg ccagtttatc taaattttct aatgtgtttt ttaacttttg
    13801 ttctacttct aactgcttaa acaattccaa agtgcgatcc atttgttttt caagatcctt
    13861 attgttgagc ttcattttat ctaacgcttc ttgcacactt tgtttgtcga gttgcgaaag
    13921 catttgctct aattgcttca tcagctcttt taattcagga ttctgcaaac ggtcaaacaa
    13981 ttcctgtatc tgttgctgtt tttcggctaa ctgactttca ttgttcttga actcattctg
    14041 cttggcaaga ttttgttgtt gctcattttt tatcttgtct aacttttcgt ccaactcttt
    14101 ttgttgcttc aacaaatctt caatacgctt gcggtcttca aagtcagttt ttttcttttc
    14161 tgtcagcttg cgtgcagcat cggtaatttg tttttgaatt tttgatgcct gtttaagact
    14221 ttcctgcaag tcttttttaa tttcatcgtt acttttctct gtattctctg ccaattcacg
    14281 aagcgtagga gctttaaaaa ttaacgattg cgatttagct gattttggcc cattgacacc
    14341 gtcattgtcc caaacttcaa aataatattc aacttcatca ccgggtacta caccatgcaa
    14401 tgcaccatcc caaaaatgat aaaaatctgt ctgattgtta ttcttgttga ttgtaagaat
    14461 ttctgtattt agtttatcgc tatgtgccga tgaatcgttt ttacttttta caaaacgata
    14521 atgaaaagcc aatttcgaaa aaccataatc atcctttaca gtccctttaa aaaatattct
    14581 ttgtaatgat gatgaatcat tttgttgttc tacctgaatg ataggatact gatcgggaat
    14641 tacgttgatg gaatattcta aagtgtcttt attggtgata aaattattgg cagtggcaat
    14701 ggaataatta gcacctgaaa gcattctttt gctgtaggtg tattgattgt tgtccgacac
    14761 caatggagtg aggctgtcat taaaccaaag tttgaatact gaggtattaa cggtgttgaa
    14821 tgtccatttc acaatagtgc ctgcaggtat taataagtcg cctgtattat tcagtgtttc
    14881 gtttttcttt tgaagatagg caggatagtt taaatcaatt ttgaatgcag aaattaatgg
    14941 attgggtact gatttcaatt cataattttc ggaataaaaa ccatcagcca tcaattgaaa
    15001 agatgtgctt tttatgatgc gatgaaaagt aaaagtaaaa tgttgtttat cttctttgtc
    15061 ggctttaatc tgtcttcctt cataagtcac ataaacttga tcgggtagtt cctgcccttc
    15121 tactttaaag ttaagtgtaa agtcattctg ttcaattaca ttaaagtttt tattgagtag
    15181 aataaaatta aaaggtgcag gttttttata cgctacattg taattgacaa gtctgcttgt
    15241 actttcttta ataacgtttg gtgctgcaaa cagcagaata attaaagtgc ctgcaggtat
    15301 taaagctaat ggcaaatatt ttctgttctt tttaaaatca atagcaccgg taaatggaat
    15361 aggtttcagg ttttgaattt tctgatcaat gccggcctga accaattcat tgtggttgtt
    15421 ttcgctgagt tcattaagtt gaagcgtatt gagtagttta tcttttacat cagtaaagtg
    15481 gttgccgata attgttgcag cttgctcgta agaaagagtt ttgcctaatt tccagatttt
    15541 aaaaagagga ataactatta atctgatgat gactgcaaga actgtaaata cataaatata
    15601 gaaaagtgct gttcggagtg aagagcccat ccatgcaaaa tattctgcca aagaaatgaa
    15661 aagaaaaaat ccaatagtta ttcctgtagc ataaatgctc cctctgatta cctgattgcg
    15721 gtaatactta cgaataaact catcgagttt ttcaatcaat agacggtaat ttcctgtcat
    15781 gtccctttgt tcaatacata acgctaaagt gtggtttcag gttatgtctg accttaaatt
    15841 ttaataaaca ttttacagcc taaaaatagt aaaaatgagc aggccaaatg ccatgcattt
    15901 gcgaatattg catgaagagg caaacaaaca aatgttaaaa gaaagtaaga tttaaagcag
    15961 tttaaacgat tccagaaatt tagttgtgta attgcctttt cggaagtctt cgtttttcat
    16021 caactgaatg tgaaaaggga tggtagtttt tacaccttca atcacatatt cacttaatgc
    16081 acgctccata gtatttattg cttcttctct ggtttgtgca atagtgatga gtttggcaat
    16141 catactgtcg tagtttgaag gaatagtata gcctgcataa acatgcgagt caacacgcac
    16201 accgtgtccg cccggaacgt gcagattggt aattctgccc ggtgatggtc tgaagttgtt
    16261 gtatggatct tctgcattga tacgacattc aatggcatgc atggtagggt agtagttttt
    16321 acctgatata ggttcgcctg atgcaatttt tatttgctct ttaataaggt cgtagttgat
    16381 tacttcttcc gttacagggt gttcaacctg aatacgggta ttcatttcca tgaagtagaa
    16441 attgcggtgt ttatccacaa gaaattcaac tgtaccaaca ccttcgtatt ttacagctaa
    16501 ggcagcttta attgctgctt cacccatttt ttcgcgcaat tcttttgtca tgaaaggcga
    16561 gggtgtttct tcaacaagtt tctgatgtcg tctttgtacc gagcaatcac gttcggagag
    16621 gtgacatgct tttccatatt gatcacctgc aatctgtatt tctatatgtc gtggttcttc
    16681 aatatatttt tcgagataca taccatcgtt tccaaaggca gcgcctgctt cctgacgtgc
    16741 actgtcccat gccgctgcaa actcttcgtc tttccacact atgcgcattc ctcttccacc
    16801 accaccggca gttgctttca ggatgacagg atactttatt tcagcagcaa tttttaaacc
    16861 ttcttcaaca ctggtaagta atccttcaga gccgggaata gttggtacac cggccttttt
    16921 catagtgtct tttgcattgc ttttatcgcc catgccatca atctgttcag gcgtggctcc
    16981 aatgaatttt attttattct tggcacaaat agcgctgaac tttgaattct ctgataaaaa
    17041 tccatatccg ggatggatag catcagaatt ggtgatttct gctgctgcaa taatattggg
    17101 gatattaaga taagaatctt tgctgggtgg aggacctata caaacggctt catcagcaaa
    17161 gcgaacatgc agactttcgc gatcggcagt agaatagact gccaccgttc taatatccat
    17221 ttctttacag gtgcggatga tgcgtaaagc aatttcacct ctgtttgcaa ttaatatttt
    17281 tttaaacata attagtttgg taatgatgaa attaaaaaag cgccaaggag cttataattc
    17341 taagaaaaat attgttgaat gaatcaggat ggatcaatta aaaacaacgg ttggtcgtat
    17401 tctacaggag tggaatgttc taccaaaact tttacaattt tacctttaat gtcactttca
    17461 atttcattaa acagcttcat tgcttcaata atacagatta cctgaccttc ttttacttcg
    17521 tcaccaacat ttacaaaatt tggtttgtcg ggcgatggag ttctgtaaaa ggtgccaatc
    17581 attggcgact tgatggtttt gtattttgaa tcatcacctg cagtttttgc ggcaggagcg
    17641 gctgcaactg caggagcagc aattacttgt tggggttgag caggtgccgg catttgcatg
    17701 actggctgaa ctgctggtgc tacatgtaca atttgctctt tggttgggtt tttaatggtt
    17761 attttaaaat ccttttgttc aaggtgcact tcactcactc cggctttggc tacaaagcga
    17821 attaattctt caatttcttt tttgttcata tactgttgtt ttttacaagt cttttcgtga
    17881 aaatgaatca attataacat aaccgctaca ataggtcatt tcaaaagaca gcatcaaaag
    17941 tagaaacttt ttacaaatat gatgaaatgg gcaaaaaaga aaaaagggca gccttatggg
    18001 ctgccctcgc ttattttggt ttcagcgctg ccggtttact ctataaccag cttatttctt
    18061 tgataattta gctcaatcat atacactcct ttagcaaatt gagcaaggct aatctgaact
    18121 gactgacgac cggaaatggt ttgaattttt tgcaccataa cagttcgtcc gctcaaatca
    18181 agaactttaa gatctttttg catgccatct ttatcagacc agaattcaat gtttactaca
    18241 tctgatgcag gatttggata catattgaag ttagctgcag tcaactcttc cgattcgcga
    18301 caattggttg ttgcattata agttttagtt gcagaaccac atgcattact tgctgttatt
    18361 gcaattacac cattgcctgt accccagtca agcgtaagac tgtttgaatt acccccaccg
    18421 gcaacataag ttgcaacagg tgcagaagga tagccccagt caagtgtata agaaccggtt
    18481 gtattgctga tattagctgt caattcaata ccttgtgtat ttgcgcacca gcttgaaggt
    18541 aaagctatta tgtcagttgg aggatttgga gctcctttta cagctacaca acgtggagca
    18601 cttgtgccgc atgtgttact tgcaacaaca caaacctgtc ccgttgtcat agtagaaaac
    18661 tgaatgttaa cagagttgtt tccttgtccg aatacattag cgcctgcagg tgctgtccag
    18721 ttgtagcttg tacctgtttg tataggtgta ctgtaagcta cagattgatt acacagtcca
    18781 tttgtaatac ctgtaattgc aggaggcacc attggtattc cgggagccac agttttgcat
    18841 tttaatgcag atgtaacgcc acaaatactt gtaacactta cacaaacatt tccaatatta
    18901 ttgtaaccat ttccaaaggt aacatttatt gagttgttgt ttgatgaccc tgaagcacca
    18961 gttggtaaag tccagttata aactgctgcg ccttgtaaag gtgttacaga gtaagtctga
    19021 cttgtatttg gacatgcagt gaaattacct gaaagattat ttggtattcc ggcagatttg
    19081 ctaagactaa tacattttga tgcactggta taacatgatg tttgtgccgc tacacaaagt
    19141 gttccgccat tccaagtcgg accaaagtca atatttacat tggttccatt gcctgtaacc
    19201 gttgcatcac cggtaatact ccactgatat tggcttgctc cagtaattgg tgcaatgttg
    19261 taagcaacat tacttgtgtt ttcacaaaca gtttgtgggc caagcactgt ggcaggcaca
    19321 gaaacaatac ctcttacttt ttgaattttt cttaaggtac ttccacatgc atttgctgcc
    19381 tgaacaccta cattataaaa tgaaccattc ggtgatccga aagtcacttg tacgttaggt
    19441 gaacttgttg tataaggtga tacatttaaa gggttgccat caaaataact acctataggt
    19501 gcatcccaaa catattgtgt agtattatta acagcattaa tcgaaaggtt tgcagtagtt
    19561 ccaacacaag catttaatgg catccctgtc attggcacag taactgctgc agaaggaaca
    19621 ccaactgtaa tagctaatga taaatcagca catggtgtgg caccacaaac atcttctgca
    19681 cgtacataat aagatgtact tgatgatgga tttactgtaa ttgatggtcc tgaaccaaca
    19741 ggcgttgtac cacaatttgt tttataccat ttccaatcag cagtagatcc taaagaacca
    19801 cctacagcag atagtgttgt tgaaccacct atacaaagtt ctgttccgtt ttgtgatgaa
    19861 acagacaatg cagcagttga tggtgtattt acaagaatta aatcttgttg ctgagtgctt
    19921 gttgcacaca tgtgagcatc ttgtaccatt acattataac ttccggcagt taagttgaga
    19981 tttgcattgt tgtttacata agtactgccg ttgtcataac tatacatgta tggtgttgta
    20041 ccacctgttg cattcattgt tatgctacca aatgttgatg gacattgctg atctgtatat
    20101 gttttggtaa actgcagcaa tgccggctcc gttattgtag ctgtaactgt attctggcaa
    20161 gcattggcat cactaacata gattgtatag gaaccggcag gaagtaaatc aaactcacct
    20221 gttgtattaa agtttacgaa gtcgtaagta taggaatatg gtcctgtacc atttgaagtg
    20281 ttggtagtta ttgatccatt gctctcacca aaacaattta cgttaatctg tgtggaaaca
    20341 tttattacag ggttcggatt tacatcaacc tgaacagttg ttgttgcagt acatagatat
    20401 tgatttgtta caacaacagt atatgttccg ttatatgcag aagtagcact aaaaatatta
    20461 gggttctgct gagaactgaa aggaagattg tttggatcat accactgata gctatttccg
    20521 cttccttgtc ccccggctaa gttatctgca ataaagttaa tatcatttcc atcacagaca
    20581 ggcgcattac tgcttgctat tgccaaaggt cccggtgttg cacttacacc aacagttgtg
    20641 tttgcggtgc aaccattagc tgttgcagca agattatatg ttgttgttcc tgttacagta
    20701 gcagaaggat tttcatctgt tgaattgaat ccgttaggtg ttgatgtcca agtataagta
    20761 atacctgaag aggtcataat ggtaactgtc catgaaacaa ttgttcctac atcgccactg
    20821 gctctgtcag acatgttaag tacccagttt cctccggcat tcattgggaa tgagattccg
    20881 ctccaatttg tatttattga accatttgta tttgaaggtg cataagttcc ggcaggaatc
    20941 gttgtagggc ccgagtttga ttctggaaga actgaacctg cacttacatc aaactcataa
    21001 tagccaccaa aattctgacc atttccaaaa cttgaagcag gtacaccaag acggtcaaac
    21061 acagttatta ctcctcctgg tgaagttact tttgcaataa catcacctga ataggtatga
    21121 gtaagattaa taccaatttt cagctgggta gcagaagcaa tagtttgtgc aggaattgtt
    21181 atcgttgaat ttactccgct tacattgtta tccggtatgc ttacgttagg tgaagcagaa
    21241 taagaaaccg gtaatccctg atttgcattt aatgttaccg cagaaccttc acatactgtt
    21301 gcatttgaaa cagtaggagt acctgttgga gcctggttta ctacaactgt tgttgttgct
    21361 gttgaagaac atccacctaa ggtaacagta acagtataag ttccattcat tgctaaggtt
    21421 gcattgctgc gtgttgggtt ttgcgtagaa cttgtatatc ctgcagggcc actccatgca
    21481 taagttgcac ctgatggact tgcattaaga tttaatgttg ttgcagtaca taccggtgca
    21541 ttgctcgatg cagatgcaat aggagcatta ttaacagcaa cagttacact tgatgtattg
    21601 gtacagccgg cagaactggt aacaacaaca gtatagctac catcatttac aatctgagca
    21661 tttgaaattg aagggttacg aattgagctt gtgaaaccat cagggccggt ccatgcatag
    21721 gatgcacccg atacggatgg tgttgaaaga ttaattgtga aaccaacaca aacaggtgag
    21781 ttgctgcttg cagttgcagt aggtaatgca ttaaccacaa catttgttgt tgcagttgca
    21841 gaacatcctg caagaggatc agttgctgtt acagtataaa tgcctgcatt tgcagccgca
    21901 gcaccgctta ttgatgggtc ttgcaatgag ctattaaatc cggaaggtcc agaccattgg
    21961 aatgttactc cagaagcaac tgcatgtagt tgtattgttt gacctgcaca tacagcacca
    22021 tcattacttg cagaaacaga tggtgcagca ttaactgtga cagaaacact attatttgtg
    22081 cttattacag aagaacaaat tccacccgga gcacctgaac ttagattggt aacagtgatt
    22141 acaggtgaac cacttgtggt taatcctgtc gctgtaaaag ttccactacc ggcagtagta
    22201 actgtcatag tagttgttaa tccggctgca gcaggtaaac tgcggttata agttactgta
    22261 taagatccaa caggtaaatc tgtaacagag cctgttaaac taataacaga agtgcccggt
    22321 aaaggacata taggagtaac agcagaaatt gctgtaatat tataagcagg acaaacccaa
    22381 gtaacaatta cctgacctct gtatccatca ccaccagtgc ggcttcccca ttggctgctg
    22441 ctgtttctac cacctgcacc accacctgac aatgctgagg catcagcgcc atcaccatta
    22501 cttgatcttc cattagcacc atttccacca ccaccggatc ctccggttcc accagtcatt
    22561 cctgatgcat taccaccgtt tcctgaagta cctgcaccac caccaccacc accggaattg
    22621 ccacccgatg ctgctgcacc attaccacca tttctggttc caaaggcacc attacctcct
    22681 gcaccatttg taggagatgt gcttccatgt tttcctccaa gaccaccact aacagttact
    22741 aagccaccaa atgaagagga gccaccatta tttccatcgg caccgcctac tccggcacca
    22801 cctgctccaa cagtaacagt tattgtacta ccgggaatta cagttacaga tgagtttttc
    22861 gcgtaagcgc caccaccacc accaccaccg gcacgtgtag gaggaaatat tcctgaggcc
    22921 acaccaccac cggcaccacc acctccccag gcttcaacct gaacagtagt tactccggca
    22981 ggaactgtaa atgttcctga tgatgtgaat gtttgtgttt gtgcaattgc tcctttaaaa
    23041 aagaacaaag cgataaaaac gaataccact tttaatgcac tgaataattt ttgcgggata
    23101 atttgttttt ccatttttag tgatttttgt tattaattaa aattttaccc aagcaattta
    23161 ggataacatc gttgctcatt gaattgtaat ttggtgaatg gcaaattttt tagggcgaaa
    23221 atcaaaaaac ataaagcgac atattccgtc cgtgctttta tttacgcgat tatgctgtaa
    23281 atagccgcac atgtggtctt taaagccatg tttttgattg ttactaaatg cgaatggaat
    23341 tactattttt ggcataaatt aaaatacaga tgaaacattt aatttggctt gttctgatat
    23401 atataatgag tgcttttagt gttaaagcac aagatccaac aaccttaatt caacgaactc
    23461 caataaaatc atggtatgcc atgaattccg gagaatttat tttttctgca ggtgaattga
    23521 agatatctga taaggcagtt tatgatccat ttggtagtat aaacactatt gctgaccctg
    23581 ataataaaat ccgttttagt ggtttttttc ataccagtca ggaacttcat tataattttt
    23641 cgaatgtgtt tggattttat acaggacttg cattgcgaaa tgttggattt atcaatacca
    23701 tgaaagtggg cggagaaaaa ttaaccatca aacaacgctc ctattcatta ggtattcctg
    23761 tagcattaaa gttaggcaat atgcaaacct attttttgac aacaggaatc gagcctgaaa
    23821 ttatgtttgc ctataaacga aaattgtttt atgatggcga taagtcaaaa aaatcacaat
    23881 ggttcagtaa tgatgtcaac gtattcaatc catcattgtt tgcacaagta aatttcaagg
    23941 gtggtatgta cattagagtt aaatattatc tcaacgattt tcttacaagc aatacagaaa
    24001 gtttcagtct gcccgataaa cctgtttctg tcagctataa agttgattca agtaaacttt
    24061 actatgtatc aataggaact agtattaaaa acaggaagat gaaaaagcct attaaaactt
    24121 cagtaggtat ttgatggatg ataaacacaa gttcttaatt ttttttagaa aacatttata
    24181 gaaaaagcac aagaaaagga ttgttgttgg tttaatagaa tgattccttc tttttccaaa
    24241 atattacccg aagcatttac actatcagct atgccaagcc aaggttcaat gcaaatgaaa
    24301 tctgcatttt ttgctgccca tattccaaga taaggaaagt ttgctgttgt gacacttaaa
    24361 ccatgagtat tattcttact ttttaattca attaaatctg attttagatt tttaaaaacc
    24421 aatgcatcat tttcaaataa actttttgaa agtggaatct ctgatgtgtt attaaaaaaa
    24481 ggtgtttcat tcttcagcaa gccatttt
//
LOCUS       DAOOOL010000022        24482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_158_length_24482_cov_394.472551, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000022 DAOOOL010000000
VERSION     DAOOOL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_24482_cov_394.472551"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..1303)
                     /locus_tag="PKU82_03240"
     CDS             complement(425..1303)
                     /locus_tag="PKU82_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81918.1"
                     /translation="MNNGLYNGLDSGLNQGRFSGLDNGLALGLFGNVRAKLDKDVIPY
                     IENAKIMRAQEIFAINYLVTNTKKFFLWDKIKAIYPFLGDTLLSNAFNLKIPIDSNSA
                     YRLNFDTNLPLITRFGVEFIGNSSSWATTNLIPSQVLIKGNLALGCYINGGNLGGSPY
                     PVTMGSSTTLNTNTDVISLAIRTTGKYFIPNGEAQPFVSSSDTTITGNYIGTALNGLA
                     NLYRNGIELGNTATSTNGGISGSPIFIGALGQNISSTYQNVRLGICFISTGLTKIDVR
                     NFDMIIQNYVSILNRR"
     gene            complement(1303..1758)
                     /locus_tag="PKU82_03245"
     CDS             complement(1303..1758)
                     /locus_tag="PKU82_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81919.1"
                     /translation="MALAAKNIPVVANVPNGGVALIAASSNTVTLGSNTNGATAYTAG
                     TAGGRVMSLTAVTDDTVTVNVFVWILRGATVIPIALVNVPLSSGNTNAARFNVDFLDG
                     VNIVGLPLDNTGKRYIPLLAGDVLRVGALANLTAAKTCWVHASGSDYQA"
     gene            complement(1742..2440)
                     /locus_tag="PKU82_03250"
     CDS             complement(1742..2440)
                     /locus_tag="PKU82_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD3 domain-containing DNA-binding protein"
                     /protein_id="HOZ81920.1"
                     /translation="MALVGIYKITSPSNKVYIGQSVNILNRKASYKRQDCKKQAKLYM
                     SLLKYGFDKHSFDILCLCDKSELNEKEKSYILEYDSFLNGLNLTSGGESCNVRSDETR
                     MKMSLAQKRNTKSKGHKHTEESKKLMSENRKGKVISETGRKNMSLVQLGKKMSDETKQ
                     KISDKLKGKKKSKEQILKFSKSMIGNTNRLGKKKTLEERLKISKSKMGSIIPLEVRNK
                     ISLTLKNKYHGISS"
     gene            complement(2662..3270)
                     /locus_tag="PKU82_03255"
     CDS             complement(2662..3270)
                     /locus_tag="PKU82_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81921.1"
                     /translation="MSIAALKASIASVIKLNGSKSIYATHLQAKLFEMIDEMHPENYI
                     SGNETASTLGATINGASAAIPNNTDLVATAENAGLLKKITWTNVKAFLKTYFDTIYQT
                     VLISGTSIKTINSTSLLGAGDISITPNATHTGEVTGTTALTVDKTAITNKTAVTIDTA
                     DYVLISDSSDSGNLKKGLVSDLGGFDDGDFTLVASFKSLYNY"
     gene            complement(3281..3925)
                     /locus_tag="PKU82_03260"
     CDS             complement(3281..3925)
                     /locus_tag="PKU82_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81922.1"
                     /translation="MTFVLKNIIFDFFGIRDKSEDLYKDVNGKGLHQRFNEMLAEDVD
                     ENEIDKTENVVKYTVDPDQCLDKFFFYREQEYGGLYSISAVPAIRRKIIKYASYLNRI
                     KGTEESYIFPLLLLGFSTVNIYEYVNTSGFDSPTTFDDEVRRFDMKCNSCGRYRIDIT
                     GSITMTNSLLNSIMNVVRYNEPINATLDGITYNGDTISIVGDFSDDFSDDFFND"
     gene            complement(3925..5592)
                     /locus_tag="PKU82_03265"
     CDS             complement(3925..5592)
                     /locus_tag="PKU82_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOZ81923.1"
                     /translation="MALTNPFIGYLDRSFLTIKQSILTRIANPVTGIPEITDHSDSNP
                     FVKRVSIWAGIAEMLGYYIDNMAKETFVHTADLYESFYRLSKAYDYRIRGRIPASGTI
                     NITLNNPAPYNITIPSETEVQTSEGIKFLTLSSAVIVTGQTNVSIPVKQWTKVVASVI
                     AISDGSQNQVYELNENVVDKSINIIVDNTIPYINVDSFVRSLPLSYHFIAGMNENAKM
                     QIVFGDDINGAIPLATKDIKVGWYETSGRNGNVGSGLINTINSVLTLPTGFTASVTNL
                     LAITNGTNAESITELRKYIPLSLRTLYRAVTRQDYIDVAELIPGVAKAGLEHSCGKTV
                     DIYIAPNGGGAPSPTLLTDAYDFMELRKMITTKHRIFGAGQVIIKHLINVRSKPQYFN
                     VDTYNEVKEAVENFYNVENQTISGKVHIGDIYQLIEGLPKVEYSTVGILTPVPYARPL
                     DTGYPILDWAKEVLISSQNATYKLVFTTSTQFNLVRNNVFVNSYSVGVLVTTNDLKFT
                     VFGTYTIGNKYEFKTYNYNSGSVDLAEPSLPVFDITEFELNVTGGLV"
     gene            complement(5595..5981)
                     /locus_tag="PKU82_03270"
     CDS             complement(5595..5981)
                     /locus_tag="PKU82_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOZ81924.1"
                     /translation="MNLEYLGVSEKFTFQPDLNSRVALVSDVESIKQSMKDILSTGIG
                     TRFYNEYYGSYLEYLTFEQNDVILKDLLIYYSIDALRTWEKRIKVLNVDVKVGVDKME
                     LLIPFLILQSNEIQSLIYPFYREITQ"
     gene            complement(6003..6710)
                     /locus_tag="PKU82_03275"
     CDS             complement(6003..6710)
                     /locus_tag="PKU82_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HOZ81925.1"
                     /translation="MSAFREFLDNMTSGGLEYFGLYYGKYEALVVDCDDPEKRGRLKI
                     SCPRIYGNEKYDKWVYPSGLFAGKNYGMHFIPQNGDVIRLTFDGGNVNFPVWEYGWWL
                     KSKSIQIADKDIYCIVTPKGHSWVIDEKNDRIYFSYKDGKVIEIKGQKISLGKNGGSA
                     EKAALGETLKKRLEEICDNQKDICDAMVAMTVTCNPPGSPSTTPINSALFAQYGARAT
                     TIKSQLIEILSEVVTLD"
     gene            complement(6710..7939)
                     /locus_tag="PKU82_03280"
     CDS             complement(6710..7939)
                     /locus_tag="PKU82_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81926.1"
                     /translation="MASSPYYIIQIKESGRNITNYVTNLHYEDCIKEDDLVELKIDGA
                     TLELIDSDDFIVGNHVIYSFGLGERVQSGQRLAVIKDIDVDYAKTISMTIKCRDTGFY
                     MKKETSNKVYTNKTSSQIVSDICNLYGYVPNIDESTFVHKSISQGNKTFHQFCNELAK
                     KEGSNGKDGSFEFYVRGNELYFQKRNLKKDSKRLFTYGDGNDIVKYFNPSYSQENEGK
                     SDSVTVAGIDKETGEQFTSKAGSKSNPETALGKVQHSFDWKGKEVGISNPAPIGEVIT
                     TGKNIAVPVDDKVAADKITSASQKDANKSQLQMEMGIDLDPTINAGDIITVSGVAKKH
                     SGNWYVTKVVHQIGSGASTVISADKNASNKSLTNNTAKTSTNTSQNNDVNETKGKNSQ
                     EQVKKIHSYNWEGKEKK"
     gene            complement(7939..8256)
                     /locus_tag="PKU82_03285"
     CDS             complement(7939..8256)
                     /locus_tag="PKU82_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOZ81927.1"
                     /translation="MAGLYLHDFDLYSNGRIIVLPSGNKLLKRDKIKYSPQEPDIYHT
                     VVAGDTLTFLAWKFYSRFTSDAPKYWKYIADVNNIKNPLDIRQYIGKNIVIPNFALIK
                     LSE"
     gene            complement(8257..8667)
                     /locus_tag="PKU82_03290"
     CDS             complement(8257..8667)
                     /locus_tag="PKU82_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81928.1"
                     /translation="MIQFVPENISNPRKANLQDVVIVGRNNEMMQYVSGSENMTLNLD
                     FYSDDVERKDVIKTINWLKSLTYNDGAPGSYRNVRVIFGELFKNDVWVIDSVDPDMSI
                     FDAENGWLPVMAKVKVSFKLDTQRNINLNDVRDR"
     gene            complement(8739..>10262)
                     /locus_tag="PKU82_03295"
     CDS             complement(8739..>10262)
                     /locus_tag="PKU82_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81929.1"
                     /translation="LFGTDAIRASNILYKEGAKGVNDMQQAMTKFTALSVATERMNNL
                     KGATEQMKGSFETMMIVIGSGFTKPLSLLVKGFQFLFDIVTSLASTKLGKVLLITFGI
                     IGAGALVLGAFVLVTNLATFASGQLALAFASLGKTAIATAFAEQGLIAGTKALTASLL
                     PLIWEFLPIIVAVASVIYLFYRMKKGFEDFNAMADGTGKKLSGISGVFQKIGGVIQAF
                     MEIWSSATDDGFTFSDNTYKALKNMGIYEFAVNMGVAFGYIRQGFQMLGKVASAIWNV
                     LQPIFGSIGNLIGTVAIIIGVVLVGAVIAWTASMIGAAIATLAATWPILLIIGIIIAL
                     VYVIQNFGKIWDWVVEKFKAGWHSMVEAGKSIFMEFIVWLGGLGIQFFNWGVSLVQSI
                     GDGIISAWEWLKGEMLSLIADLPGGKILLSALGIDVPEANGTGNGGNVSGPTPLTTPS
                     AIGTTIAQQKSVRGENTNSFTQTVEKKETLQTVKLQIDGRELKTALDKVDKEQNSRK"
     assembly_gap    10263..10362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10363..10751)
                     /locus_tag="PKU82_03300"
     CDS             complement(<10363..10751)
                     /locus_tag="PKU82_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOZ81930.1"
                     /translation="MIFGGGSVGGGAIGTGIVFTLYDQFSATSDKIATKFKTLEGISE
                     QAANKIDSSLSKMKLGFASMVVGAGILATLAFPIDKAAEFEQGLSAIKAVSGATNAEM
                     KILSDLSLEMGKKTKYSALESAQGIEE"
     gene            complement(10756..10929)
                     /locus_tag="PKU82_03305"
     CDS             complement(10756..10929)
                     /locus_tag="PKU82_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81931.1"
                     /translation="MGFRVMDAWFDLSYGGLSISLEELYAMPYDNIYSLLMRMDIQKK
                     HEKAEMDKAKHKK"
     gene            complement(10941..11693)
                     /locus_tag="PKU82_03310"
     CDS             complement(10941..11693)
                     /locus_tag="PKU82_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81932.1"
                     /translation="MLLECVTRIGSNSSPNLKDIQNLLSEDRKEMLMALRQLSNGFNP
                     TFTFKYEFPTQNSKRLEQTYEVIFDKEDFPKKPYLWTLQAMEKSFKEEHGIPEQDELT
                     NEQKNEVMQLPFPVLFENYDEMLKLYKKQTLKLPESGVTVVYNLLDGKTENNYGKTIK
                     SNKISSHTYIVMRDAKYLDPDKLEQGKEIPIILPLGELSIVDIEFLRGEMRKVEADIH
                     TTVVVQYKGDASIQAQVNLIATPAFFFPSLAL"
     gene            complement(11988..12251)
                     /locus_tag="PKU82_03315"
     CDS             complement(11988..12251)
                     /locus_tag="PKU82_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81933.1"
                     /translation="MEKSKKNSHMMNAETWRAYMYMKVTLENIYSGKYPNGVKFKPKD
                     YEDAKEQVELCTQVLMDHNVDLPIYEAPVFEPVAGSQLDLFMV"
     gene            complement(12257..12640)
                     /locus_tag="PKU82_03320"
     CDS             complement(12257..12640)
                     /locus_tag="PKU82_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81934.1"
                     /translation="MKAVLSQQNGSSMHSYHNTTGMQKPELKAAEQKAQKQDVLVLQV
                     FKDNRLKDFTPCEVYEYMIKKGVISRKVPITSIRRAISNLTATGKEDLEKLPGKREGA
                     YGQPNRTWRLNRVKYNVAEQMEMFN"
     gene            complement(12652..13122)
                     /locus_tag="PKU82_03325"
     CDS             complement(12652..13122)
                     /locus_tag="PKU82_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81935.1"
                     /translation="MYGLKATTKSNYCLVMVWQKDSLDNNRHILLHLAPELRAKLQVA
                     FSIIDGAPVFNPNIDKSNLQFTDIGFDITKHVKLIGNLIDGSTDAIDEMFTESDKILF
                     VEKVDTSEFAEYFTEVRQAILHLKDELFSIEIIGVNEEHDLWLADAIETKFIKH"
     gene            complement(13222..13380)
                     /locus_tag="PKU82_03330"
     CDS             complement(13222..13380)
                     /locus_tag="PKU82_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81936.1"
                     /translation="MKIKDFIKVFGESGHSARFEGCTAKNGDKATGEYKVEANEVLYT
                     YTDKKERT"
     gene            complement(13673..13861)
                     /locus_tag="PKU82_03335"
     CDS             complement(13673..13861)
                     /locus_tag="PKU82_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81937.1"
                     /translation="MKKELKQAIINFIFENEKDFQLNNRTSNEFSAYMYDSSGNYLIG
                     GEDVARFIRDAIKLLTEQ"
     gene            complement(14039..14392)
                     /locus_tag="PKU82_03340"
     CDS             complement(14039..14392)
                     /locus_tag="PKU82_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81938.1"
                     /translation="MLSHTIKKTAKKLFSGKYKGLFMLLEINQHAISFKNVNGVVFSY
                     QSGPDIENFFNLFPGCGFLSAGYVKEGTVYGPTFKSEYNTCGLIDTAEYTAFYQQATD
                     NPDFIKRTALQITLN"
     gene            complement(14429..14728)
                     /locus_tag="PKU82_03345"
     CDS             complement(14429..14728)
                     /locus_tag="PKU82_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81939.1"
                     /translation="MNNYNSIKAKIQNEGCYIEAETYKNGVTIHSLIKREDETTKTIF
                     CEIAANIAKMLEKETGKAITHKEGLTVALNFNPVPVKHNMVRTFHYNDTEEKLFN"
     gene            complement(14793..15086)
                     /locus_tag="PKU82_03350"
     CDS             complement(14793..15086)
                     /locus_tag="PKU82_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81940.1"
                     /translation="MNTATATTAQEINLISYNIHGQKLREGIIKIDFEKEKVIFPSGK
                     EYSVINDYEFTYKILKGTIKAAGGINGLSKFFNEINRQDSPFNPCYTNLFIEL"
     gene            complement(15097..15321)
                     /locus_tag="PKU82_03355"
     CDS             complement(15097..15321)
                     /locus_tag="PKU82_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81941.1"
                     /translation="MQTPPSQEVQTAINENNKVLDFIKTWQSKNEYNPKFLDEFNAKF
                     NAEFNDFLKAGNFTAETIKFFIYKPDLITK"
     gene            complement(15792..16232)
                     /locus_tag="PKU82_03360"
     CDS             complement(15792..16232)
                     /locus_tag="PKU82_03360"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOZ81942.1"
                     /translation="MAINNPQGNFNWRLDLDDVSNERLQNVQLPSVEWTEHKQGTQGN
                     DPDTKTPGKKIVGDMTAEMVVPDTGDTDLWNKLNKAKTMKRSQYCGNGVLYELDANGQ
                     PVQTHILENVWIKKVETANYETKGDNSDNLIRTVTFSVGDTNVQ"
     gene            complement(16243..18204)
                     /locus_tag="PKU82_03365"
     CDS             complement(16243..18204)
                     /locus_tag="PKU82_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81943.1"
                     /translation="MTNLSGTPGASTNISDVGVIPANSNKGIICVNIIAKRGKLGKQY
                     LITNATELKRILGGAIPGNQDFYKLSRGLNKGAKLRVARVFHLTDQDDATTVEGDVAT
                     VTATQNVTEVLARANAKLTTVPSGDVVVQYVVNTVSGEVVIASYTIPTSGSPTVATVN
                     TALYTAVNAGTGTHGYTATIDGGYIVTVIAPTGSGEDANRYFLNVVQTGATAGTVAGN
                     GRFAGGVTGVDASVEIDAEAVGSGYNGTVIEIVKSISNQPGKVDIKVTLPDNDLPIQV
                     SNYDRVLTSETCASLNGQIGVVAFDFSSMNVGNMLPIGTFILADGTQDVSLINDADFI
                     GSPISKNGYHSFNDVSDSMRIWNVSKPTHTANQGLVAYVMARGDMRCRIYTPLGLSIE
                     GITDFRNGTGSFSHQPIDSFYGDMWYTDIEINDPANSSNTGYVISGIGDQLANRTLAD
                     DKAGVWISDSGEMFGKLFGVNDVAINFKSPAYKGYYDDLYEGGVNAIVNHPTFKITNW
                     GNRTLNLNRTSLLSKTNIADLVVYISREVKLIAERMSFNPNDLVMFGRLYRKVRPFII
                     DELIAGRAIEGGTGPKYGEGTWWHWLGDQNAKNLNELQFNTKSDVDAGKYRVRFAFKP
                     IASNEYIAIDIAPADSVTIQNIQQLLTVN"
     gene            complement(18261..19013)
                     /locus_tag="PKU82_03370"
     CDS             complement(18261..19013)
                     /locus_tag="PKU82_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81944.1"
                     /translation="MKFKQSEISDGIFEVIRKAVVAGGYLPDFVSAGSSSAYQSAKGA
                     IISSGKEVIEVFNVGTYKSKGEDMNNNIIINRASVVPAKIGTGKEIAFVQNDNGTFDK
                     IHTPDTRYDIMYSISYNTISEKYAEIIEDIILNAFESRKFIRAVRSNGDIIMNVLDDY
                     NNDLSDDFGGSIESIENVAGFWLMNRGFVDTSGTNFIEKQWRFEAINIDLIGWKNIDT
                     VPQLVQFDFALGTEPNNTNAGNEDLFNIDLEI"
     gene            complement(19010..19504)
                     /locus_tag="PKU82_03375"
     CDS             complement(19010..19504)
                     /locus_tag="PKU82_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81945.1"
                     /translation="MAKNIKKVGRWDLAGMVTKNLKHDIKDSNLIVNRKMGSLLERYV
                     IKWIKRQPGLWAPLNEKYKKRKEKQGYSNLTLRRTGTLINNITSVASMDTIFCGVKRE
                     VKYNDGESVANIALIMEVGSAKRNIPPRPYLTPAMRWMGMKIRTEKLYEVMLMDYIRK
                     KYGI"
     gene            complement(19497..19952)
                     /locus_tag="PKU82_03380"
     CDS             complement(19497..19952)
                     /locus_tag="PKU82_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81946.1"
                     /translation="MASLLTDSEFDEIRDTIKDVTDTFLRLPIVWKKDKNTATRFMRD
                     IDGERTYDDIALSGLVVWNDQGNGALLSKGLNGAIDLADGYVLLNYEDAQTAGVVSST
                     HNQIVNPAKDYLVFDNEKHEILSLNMLGQLKDKNCLLKVHFKKNIRPNG"
     gene            complement(19955..20500)
                     /locus_tag="PKU82_03385"
     CDS             complement(19955..20500)
                     /locus_tag="PKU82_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81947.1"
                     /translation="MAIGTVIQLVKERLPFIPNDNTYDARIESLKLEVYYALQHCTKL
                     TDAEVEDDSKYTGLRRFLVARYVCYLLIYNKIIEVVGGVGGSAPSGNKRIKKGKADVV
                     EAEFDYAKASDGTTLTMEATNLMNGLLKEICSYASTLKCSLPLCSGIIKSKGYGPAFI
                     AYGGTSNICGCGSSSNPQNDY"
     gene            complement(20570..21019)
                     /locus_tag="PKU82_03390"
     CDS             complement(20570..21019)
                     /locus_tag="PKU82_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81948.1"
                     /translation="MEIFVKLKDASNSHSILHQQKNLFGKKVEKVLKDVYVSKLLKDG
                     VIVEAKETEYDAWVKSNPVEVSAEEKAIEDNKVSEKSEVEKTEETTADETFVDLAEKF
                     IDQGIVTKDSKGYKLESEYIGKKKNDVAKSLSELPAEKLEELKALLD"
     gene            complement(21045..22391)
                     /locus_tag="PKU82_03395"
     CDS             complement(21045..22391)
                     /locus_tag="PKU82_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOZ81949.1"
                     /translation="MKENLLKLRKHNLAVFKGDKTDRLQADEVKTLTEETIPDFAKEI
                     ALTRQGRATDAYGRVIPAYNISFADAISIHYGIQPAKQTESGEKLSYKQQQKHVVKEF
                     LKQMDIFMGSDTLKTAAARLGNANLGVQSLTDALTKYSQFDALANTSGVPADYRFIIP
                     ELILTAIRLDYDASSLHQGWVATTQSISQDEITMPFIKKGNAVARKVGEGESIEFGSV
                     SFGKKKAGVFKVGLGFKITDELIERSSLDMIFTFLGEVGVDMSICADYEAYRVLVNGE
                     QSSGAESAPTIGVATANTFNFKDIKRGTSRLRRLKRMPDTIITGENDGIDITSLPEFQ
                     GFNGGNKMATIQSILGVPDTLKNDVYTVGSNQIMLLDSTKAMAKLQYGSMKMEERRNP
                     QTQENEIFVSDYIGFSIIRRDARLIIDKSVLFSAQGFPAYMDIDARINEAFKYHEE"
     gene            complement(22434..22877)
                     /locus_tag="PKU82_03400"
     CDS             complement(22434..22877)
                     /locus_tag="PKU82_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81950.1"
                     /translation="MTGSIQTTPTQVIKNMTSDALQLTFVSSAGSEIKAGQEVYQHTD
                     GTVKKRTLGTQIPLGIALSDTPDGERVLVRVPHQMVIEGIAKGGALNAGVAVVPDGTL
                     NADGYPNYVAAGASAYVSAFTLKAAVEDARVRIGIIQGVYLTPGA"
     gene            complement(22913..>24482)
                     /locus_tag="PKU82_03405"
     CDS             complement(22913..>24482)
                     /locus_tag="PKU82_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81951.1"
                     /translation="YAVLIYNDFFIILRDKVKLLMSKKGKITMFNNTNGLLLAPKFIE
                     LDSGKKQSFSWDNEDYRQSVVNPDISNAIAKKEDFLAFNFRHISATIVGAWSWKATEF
                     NEKVLKEAKSLLEYKPALVNHNAETGNIIGVNGKVVYENAYKANDGTIIPAGLNAPIW
                     IDGKLHTDICRKLSAFPVPHIQSVSVSLLFEWEPSHTFTDQSGNEDDWMFEMRIGTLV
                     DGTMVRRVVTKIVDFYETSLVFLGADPFAKIVDTEGKLVNVEKSAIVGRQMFSDDKIG
                     SDYYKRSNGHYYLMDDVTFANEKSINLRERINVDFSKNGNSPKNNEMKVLKLSGKKED
                     YAQEVLTTLEESFKVEFVELKDETETITNLTKEVEEFKSAKTTAEESLAKVQSELTEA
                     KNAFAEYEKIVPVAELSEFAKVIDLAQVKPLAEFGRKVELSKREECIRLYKVSVKDKT
                     NDAVIKTINEASFDALEGLMQQYGGTCMEQFGGKCDECNSTKVSFRSSVNEGEQKEET
                     EKTSFVSMAEQIRN"
BASE COUNT         6717 a         5178 c         3796 g         8691 t
ORIGIN      
        1 cctgtgtatt cgtacttatt tttactgctt aaatgtttgt gccaatattc gtatggagtt
       61 ttatttaact tggactcctc acttaggtcg catatcattt ttgcatagcc ggaaatacag
      121 ccaagtgcgt gagcaaacac ggaaaataca actgataata tgattaacgt tgttgtcata
      181 agttcataaa ttttttggtt gtgttataaa tagttatatt ccgaaccttc ctcttaaatt
      241 attaaagtta ttcataactt cttctttact caaagctttt ttataaaact ttaatactgc
      301 aacatcagcg ttagtatggt aagaatctaa ataactatta tgtattctaa tcttttttcc
      361 tgaatgattt ataatagaat tttcattttt gatattgttt agtttttttt aaatattaaa
      421 tagtttatct tctatttaat atactaacat agttttgtat aatcatatca aaattacgta
      481 catctatttt tgttaatccc gttgatatga agcaaatacc taatctgaca ttttgataag
      541 tactacttat attctgccct aatgcaccaa taaaaatggg tgaaccagat attccaccat
      601 ttgtacttgt tgctgtattt cctaattcaa taccatttct atatagattt gctaaaccat
      661 tcaaagctgt gcctatatag ttacctgtta tagttgtatc agaagaagat acaaaaggct
      721 gtgcttcacc atttggtata aaatactttc ctgttgttct tattgctaaa ctaattacat
      781 ctgtatttgt gtttaaggta gttgatgaac ccatagttac aggatatggt gaaccaccta
      841 aattgcctcc gtttatataa caacctaatg cgagatttcc ttttatcaaa acttgtgaag
      901 gtattaaatt ggtagtagcc caagaagaac tattaccaat aaactctacg ccaaatcttg
      961 taattaaagg aagattagta tcaaaattta atctataagc agaattggaa tcaataggta
     1021 tttttaaatt aaaagcattt gataacaaag tatcacccaa aaacgggtaa atagctttga
     1081 ttttatccca taaaaaaaac ttttttgtat tagtaaccaa ataatttatt gcaaaaattt
     1141 cttgtgctct cattatttta gcattctcta tataaggaat tacatcttta tctaatttag
     1201 ctcgtacatt accaaacaac cctaaagcta aaccattatc taatccggaa aatcttcctt
     1261 gatttaatcc gctatctaaa ccgttatata atccgttatt cattacgctt gataatcact
     1321 acctgatgca tgtacccaac aagtttttgc tgccgttaaa ttcgctaatg caccaactct
     1381 taaaacatca cctgctaata aaggaatata tcttttacct gtattgtcaa gcggtaatcc
     1441 tacgatattc acaccgtcta aaaagtccac attgaaacgt gctgcattcg tattacctga
     1501 actcaaagga acgtttacaa gtgcaatcgg tataacagtt gcacctctaa gaatccatac
     1561 aaatacgtta accgttaccg tatcatcagt aactgccgtt aagctcatta cacgaccacc
     1621 tgctgtgcct gctgtgtatg ccgttgcgcc gtttgtatta ctacctaatg ttaccgtgtt
     1681 tgaagatgct gctataagtg ctacaccgcc gttaggcaca ttcgccacaa cagggatatt
     1741 cttagctgct aatgccatga tatttatttt ttaatgttaa tgatatttta tttcttactt
     1801 ccaatggaat tatactaccc attttgcttt tagatatttt taatctttcc tctaatgtct
     1861 tttttttgcc taatctattt gtgtttccaa tcatagattt actaaacttt aatatttgct
     1921 ctttgctttt tttctttcct tttaatttgt cgctaatttt ttgctttgtt tcatctgaca
     1981 tctttttgcc aagttgaacc aaactcatat tttttctacc agtttcgcta attacttttc
     2041 cttttctatt ttcactcatt agctttttag attcttccgt gtgtttatgt cccttacttt
     2101 tggtgttacg cttttgtgct aaactcattt tcatccttgt ttcatctgac cgcacattgc
     2161 aactttcacc accacttgtt aaattcaaac catttaaaaa gctatcatat tctaaaatgt
     2221 atgatttttc tttttcgttt aattcacttt tatcacacaa acataaaata tcaaaagagt
     2281 gtttatcaaa accatacttc aaaagtgaca tatatagttt tgcttgtttt ttacaatctt
     2341 gtcttttgta agacgctttt ctatttaata tattgacaga ttgacctata tacaccttat
     2401 tagatggtga cgttatttta tatatgccaa ctaatgccat aatattatat taaaatgtct
     2461 aaaaatgtta ttttacttag gaatcctatc gtttcaatct ttatgattaa accctttttc
     2521 caagctaatt gctgaatgtc tttctcgttc aactcatccg tgacaaagta taataagtca
     2581 cttctgttag ttgctgagtt cttccaatct atcagcttat tatcgtagtt attttgattt
     2641 atttttacgt ttggcatgat attaataatt ataaagtgat ttaaaagatg ctaccaatgt
     2701 gaaatccccg tcatcaaacc cacctaaatc agaaactaat ccttttttaa gattgccaga
     2761 atcggaacta tctgaaatta aaacataatc agccgtatca atcgttactg ccgtcttgtt
     2821 tgtaattgca gttttatcaa cagttaaagc cgttgtaccc gtaacctcgc ccgtatgtgt
     2881 tgcatttgga gttatagata tatcgcccgc accgagtaac gatgttgaat taatagtttt
     2941 tatcgaagta cccgaaatta atacagtttg atagatagta tcaaaatatg tctttaaaaa
     3001 cgcctttaca tttgtccaag taatcttttt aagaagtcct gcattttctg ccgttgctac
     3061 taagtctgta ttgtttggta ttgcagcaga agctccatta atcgttgcgc ctaaagtaga
     3121 agccgtttca ttaccgctaa tataattttc aggatgcatt tcatctatca tttcaaataa
     3181 tttggcctgt aagtgtgtag cataaataga tttactaccg tttaatttta ttacggatgc
     3241 tatacttgct tttaatgctg ctattgacat attttattta ttaatcatta aaaaaatcat
     3301 cactaaaatc atcactaaaa tcacctacaa tactaatagt atcaccatta tatgtaatac
     3361 cgtccaaagt cgcatttata ggctcgttat acctcactac attcattatg gagttaagca
     3421 atgagttcgt cattgtaata cttcctgtaa tgtcaatcct gtaccggcca caactgttac
     3481 atttcatatc gaatcttcgt acttcatcat caaaagttgt tggactatca aatcctgaag
     3541 tattcacata ttcgtaaatg ttaacggtac tgaatcctaa taaaagtaat ggaaaaatat
     3601 aactttcttc agtacctttt attctattta aataagatgc atattttata atttttctcc
     3661 ttattgcagg tacggcagat attgaataca aaccaccata ttcctgctca cgataaaaaa
     3721 agaacttatc taaacattgg tcgggatcta ctgtgtactt taccacgttc tccgtcttat
     3781 caatctcatt ttcgtcaaca tcttcagcca acatttcatt gaaacgctga tgcaatcctt
     3841 ttccatttac gtctttgtat aaatcttcag atttatcacg tattccgaaa aaatcaaaaa
     3901 ttatattttt aagtacgaat gtcattatac caacccacct gttacattta attcaaattc
     3961 tgtaatatca aataccggta aactcggctc tgctaaatcc acacttcctg aattatagtt
     4021 gtacgtctta aactcatatt tatttcctat tgtgtatgta ccgaacactg taaactttaa
     4081 atcatttgtt gttactaaaa ctcctactga atagctattt acaaaaacat tatttctaac
     4141 taaattaaat tgtgtactag tcgtaaaaac taacttgtaa gtagcatttt gtgacgatat
     4201 caaaacttct ttcgcccaat cgagaatagg ataacctgta tcgagcggcc ttgcataagg
     4261 aaccggagtt aaaataccta ctgttgaata ttcaactttt ggcaatccct caattaattg
     4321 atatatgtcg ccaatatgca cctttcctga tatcgtttga ttttctacat tatagaagtt
     4381 ttcaactgcc tctttaacct cattatacgt gtcaacattg aagtattgag gctttgacct
     4441 tacatttatc agatgcttaa taataacctg ccctgcacca aaaatcctgt gctttgtggt
     4501 tatcatttta cgcagttcca taaaatcgta tgcatctgtc agcaatgtag gacttggtgc
     4561 tcctccaccg ttcggtgcaa tgtagatgtc caccgtcttt ccgcaggagt gctccaaacc
     4621 tgctttcgct acacctggta tcaattctgc tacgtcaata taatcctgcc tcgttacggc
     4681 tctgtataag gtacgcaatg ataacggtat gtatttcctc aactcggtaa tactttctgc
     4741 attggtgccg ttcgtaatgg ctaacagatt ggttactgaa gccgtaaaac ctgtcggcaa
     4801 tgtcaatact gaatttatgg tattgataag gccggaaccg acgttaccat ttctaccact
     4861 cgtttcatac caacctactt tgatgtcttt tgttgccaac ggtattgctc cattgatatc
     4921 gtcaccaaaa actatttgca tttttgcatt ctcattcatt cctgcaatga aatggtaact
     4981 caacggtaaa ctccttacaa aactgtcaac attaatatac ggtatcgtgt tgtcaacgat
     5041 aatattaata cttttgtcaa ctacattctc attcagttca taaacctgat tttggctgcc
     5101 gtcggaaatt gctattacag atgcaacaac ttttgtccat tgcttaaccg gtatgctcac
     5161 attcgtttgg cctgtaacga ttactgcaga actcaatgtc aaaaacttta taccctctga
     5221 agtttgtact tccgtttcgc tcggtatggt aatattgtat ggtgccggat tattcagcgt
     5281 gatatttatt gtgccacttg caggaatacg gccacgaata cgataatcgt atgcctttga
     5341 tagcctgtaa aaactttcgt ataaatctgc agtatggaca aatgtttcct ttgccatatt
     5401 gtcgatgtaa taacccaaca tttcggctat tccggcccat attgatactc gctttacaaa
     5461 cggattgcta tctgaatggt cggttatctc tggtatgcct gtaaccggat ttgcaatacg
     5521 tgttaaaata gactgtttaa tcgtaaggaa cgacctgtct aaataaccta tgaacggatt
     5581 tgttaatgcc atttttattg tgttatttct ctgtaaaatg gatagattaa tgactgtatc
     5641 tcgttcgatt gcaggattaa aaacggaata agtaattcca ttttatcaac tccaactttt
     5701 acgtcaacat tcaatacttt aattcttttt tcccacgttc tcaatgcatc gattgaataa
     5761 taaattagta aatcttttaa tattacatca ttctgctcaa acgttagata ctccaaataa
     5821 ctaccatagt attcattgta aaatcttgta ccaatacctg tgcttagaat atctttcatt
     5881 gattgtttta tactttccac atctgatact aatgctaccc tgctattcaa atcaggttga
     5941 aacgtaaatt tctcactaac acctaagtat tctaagttca taaatacaat ataatgaaaa
     6001 ttttagtcta atgtaacgac ttcagatagt atttcaatca actgtgattt tatcgtcgtc
     6061 gctcttgctc cgtattgtgc aaataacgca ctatttattg gcgttgttga cggtgaacct
     6121 ggtggattac acgttactgt catggctacc attgcatcac aaatatcttt ttgattatcg
     6181 catatttcct ctaaacgttt ttttagagtt tcacccaatg ctgctttctc ggcagaacct
     6241 ccgttcttac ccaaacttat tttttgtcct ttgatttcaa ttacctttcc gtctttgtag
     6301 ctgaagtaaa tcctgtcatt tttctcatca attacccaac tatgtccttt cggagtaaca
     6361 atgcagtaaa tgtctttgtc ggcaatctga atgctctttg acttcagcca ccaaccgtac
     6421 tcccacaccg ggaaatttac gttacctccg tcgaatgtaa gcctgataac atcaccattt
     6481 tgaggtataa agtgcatacc atagttttta cctgcaaata aaccacttgg ataaacccac
     6541 ttatcgtatt tttcattgcc gtaaattctc ggacaggaaa tttttaaacg tccacgtttt
     6601 tcaggatcgt cgcaatctac taccaatgcc tcatactttc catagtataa accgaagtat
     6661 tccaaacctc cacttgtcat gttatctaaa aattctctga aagcgctcat tatttttttt
     6721 ctttaccctc ccaattataa ctatgaattt tctttacctg ttcctgactg tttttgcctt
     6781 ttgtttcatt cacatcattg ttttggcttg tgtttgtact cgttttggca gtattattag
     6841 tcaatgattt atttgatgcg tttttatcgg cacttatgac ggtacttgca ccactaccaa
     6901 tttgatgcac cacttttgtt acataccaat taccggaatg tttttttgct actcctgata
     6961 cggtaataat atcacctgca ttaattgtcg ggtccaggtc tatacccatt tccatttgca
     7021 actgtgattt atttgcatct ttttgtgatg ctgaagttat tttatctgca gctactttat
     7081 catctaccgg aacggcaata tttttaccgg tagttattac ttcaccaata ggtgcaggat
     7141 tgcttatacc aacctcctta cctttccaat cgaatgaatg ctgaacttta cctaatgcag
     7201 tttcgggatt gcttttacta ccagctttag atgtaaactg ctcacctgtt tctttgtcta
     7261 tacctgcaac ggtaacggaa tcagattttc cctcattttc ctgactgtat gacggattga
     7321 aatactttac aatatcatta ccgtcaccat aggtaaacag tcgcttacta tctttcttca
     7381 ggttacgttt ctgaaagtat aattcattac cacgaacgta aaattcaaaa cttccgtcct
     7441 taccattact tccctctttt ttagctaact cattgcagaa ttggtgaaac gttttatttc
     7501 cctgtgatat gctcttatga acaaatgtac tttcgtcaat atttggcaca tatccgtata
     7561 aattgcagat atcgctcact atttgtgaac tcgttttatt cgtgtaaact ttattcgacg
     7621 tttccttttt catgtagaag cctgtatctc tacattttat cgtcatggaa attgttttgg
     7681 cataatccac gtctatatct ttaatcactg ccaacctttg gccggactgc acacgctcac
     7741 ctaatccgaa actgtatatc acatgattac cgacaataaa atcatcgcta tcaatcaatt
     7801 ctaatgtggc tccgtcaatt ttcaactcta ccaaatcatc ctctttgatg caatcctcat
     7861 aatgcagatt tgtaacatag ttcgttatgt tcctgccact ttcttttatc tggattatgt
     7921 aatatggact gcttgccatt attcgcttaa tttaatcaat gcaaaattcg gtattacaat
     7981 attcttacct atgtactgcc ggatatcgag aggatttttg atgttgttaa cgtctgctat
     8041 gtatttccaa tacttaggag catctgaagt aaatctactg taaaatttcc atgctaaaaa
     8101 tgtaagtgta tctcctgcaa ctactgtatg gtaaatatca ggctcttgtg gagaatactt
     8161 tattttatca cgtttcagca atttattacc acttggcaga actattatcc ggccattact
     8221 gtataagtca aaatcgtgta aatataaacc tgccatttat ctatctctta catcgtttaa
     8281 attaatattt ctttgcgtat ctaatttgaa actcactttt acttttgcca ttacaggcaa
     8341 ccaaccattt tcagcatcaa atatactcat gtcgggatct acggaatcaa ttacccaaac
     8401 gtcgttctta aacagttctc cgaatatcac tctaacattc cggtaactac caggtgctcc
     8461 gtcgttatat gtcaatgatt tcagccaatt tatcgtttta atcacatctt tcctttcaac
     8521 gtcgtcactg taaaagtcta aattcaatgt catattttca ctaccgctca catactgcat
     8581 catttcatta ttacggccta cgattaccac gtcctgcaga tttgctttcc tcggattact
     8641 gatattttca ggcacgaatt gtatcataca cctttggaac ggaggccgta attccacaat
     8701 gtataattca ctctttgttg tattgaaatt gtactgcatt attttcgaga attttgttct
     8761 ttatcaacct tatccaatgc agtttttaat tccctgccgt caatctgcaa tttaaccgtt
     8821 tgtaacgttt cttttttctc aactgtttga gtgaatgaat tagtattctc accacgaaca
     8881 cttttctgtt gagcaatagt ggtgcctatt gcagacggag tggttaacgg agttggtcca
     8941 ctaacatttc caccattacc ggtgccgttt gcttcaggaa catcaatacc caatgctgaa
     9001 agtaaaatct tacctccggg taaatcggca atcagggaaa gcatttcacc tttcaaccat
     9061 tcccacgctg aaataatacc gtcaccgatg ctctgcacaa gtgaaactcc ccaattaaag
     9121 aactgtatgc ctaatccacc caaccaaact atgaactcca taaaaataga ctttcctgcc
     9181 tccaccattg aatgccaacc tgctttgaat ttttctacca cccaatccca aattttaccg
     9241 aaattttgaa taacgtaaac gagtgcaatg attattccga taatcagtaa aataggccat
     9301 gtggccgcca atgtagcaat ggccgctcct atcattgaag ccgtccatgc tattactgca
     9361 cctaccaata ccacaccgat aataattgcc accgttccaa tcagatttcc tattgaacca
     9421 aagatcggct gaagtacgtt ccatattgca gatgccactt tacccaacat ttggaaaccc
     9481 tggcgaatgt aaccgaatgc tacacccatg ttgactgcaa actcatagat acccatgttc
     9541 tttaaagcct tatacgtgtt atcagagaac gtaaatccgt cgtcggtagc actactccaa
     9601 atttccatga acgcctgaat tacaccaccg attttttgga atacaccgga gatacctgat
     9661 agctttttac ctgtaccgtc ggccatagca ttgaagtcct caaacccttt tttcattctg
     9721 taaaacagat agataacgga tgctactgca actataattg gcaggaactc ccaaatcaat
     9781 ggcaggagtg atgccgtcaa cgctttagta cctgcaatca atccctgttc agcgaatgca
     9841 gttgcaatgg cagtcttacc caaagatgcg aatgccaatg cgagttggcc ggatgcaaac
     9901 gtggctaaat ttgtcactaa tacaaacgca cctaatacca atgcacctgc tcctataatt
     9961 ccaaacgtga tgagtaatac cttaccgagt tttgtacttg ccaaagatgt aacaatatcg
    10021 aacaaaaatt ggaaaccttt tacgagcaaa gataacggct ttgtaaatcc tgaacctatt
    10081 actatcatca tcgtttcaaa cgaacctttc atctgctctg ttgctccttt taggttattc
    10141 atacgctccg ttgcaacact caatgcagta aactttgtca tggcttgttg catatcattc
    10201 acgcctttcg caccctcttt atacaggata tttgatgctc tgattgcatc ggtaccaaat
    10261 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaactcctc aataccctgt
    10381 gctgattcta atgcagaata ctttgttttc ttacccattt cgagtgataa atcactaagt
    10441 attttcattt ctgcattggt agcacctgat accgctttta ttgcggaaag tccttgttca
    10501 aattctgctg ctttatcaat aggaaatgcc aaagttgcta atatacctgc acctactacc
    10561 attgaagcaa aaccaagttt cattttcgat aaactactgt caattttgtt tgctgcttgt
    10621 tcagatatac cctccaaagt tttgaacttt gtggcaatct tatcagatgt tgcactaaat
    10681 tggtcgtaca acgtgaatac aatccctgtt ccgattgcac caccacctac gctacctcct
    10741 ccgaatatca tacttttact ttttgtgttt tgctttatcc atttccgctt tttcatgttt
    10801 tttctgaatg tccatacgca ttaaaagcga gtaaatatta tcgtatggca ttgcatataa
    10861 ttcttcgagt gaaattgaca gtcctccata acttaaatcg aaccacgcat ccataacccg
    10921 gaatcccaac tcgtaccctt ttaaagtgcc aagctgggga agaaaaaagc cggagtagct
    10981 atcagattta cctgcgcctg tatagatgca tcgcctttgt attgtaccac cacagtagta
    11041 tgaatatcgg cctccacctt tctcatttca ccacgtaaga actctatatc aacaatcgac
    11101 agttctccca atggcaagat aattggtatc tccttgcctt gctccaactt gtcgggatct
    11161 aaatactttg cgtcacgcat cacgatatac gtgtgggaac ttattttatt ggacttaatt
    11221 gttttgccat agttgttttc agtcttaccg tccaacaggt tataaactac tgtaacaccg
    11281 ctttcaggca gtttcaatgt ttgcttttta tacaacttca acatttcgtc ataattctca
    11341 aacaataccg ggaatggcaa ctgcataact tcgttttttt gctcgtttgt cagttcgtct
    11401 tgttccggta ttccatgctc ctctttaaac gatttttcca ttgcttgtaa cgtccatagg
    11461 tacggtttct tagggaagtc ctctttatcg aaaataactt catacgtttg ctctagtctt
    11521 ttactgtttt gagtcggaaa ctcatactta aaagtgaacg tcggattaaa tccgttcgac
    11581 aactgacgga gcgccataag catttccttt ctgtcttcag acaacaggtt ctgaatatcc
    11641 tttaaattag gacttgaatt acttccaatc ctggtaacgc attcaagcaa catagtatcg
    11701 attgcatcac gacgtttagc ctcgtcatta atcgaaatat cctcttggtg cttacctctt
    11761 agggattgca gttcaacctc cacaccggag ggaattacgt gtgtaccacg tttacgattt
    11821 ccaaaaacat tagacataat tataaatttt tcattggtga ataatatcaa atataaacaa
    11881 aaaaaagaga gatgcgaaca cctctctaaa cacttaaaaa acaatatgtc tgtcagcaag
    11941 tcaaacacat atctttataa gtttgcattt ctgcaaatat tatattttta taccataaac
    12001 aaatccaact gtgaacctgc aaccggctca aaaaccggtg cctcatatat cggtaaatct
    12061 acattgtggt ccataagaac ctgcgtacat aattccactt gttctttagc atcctcataa
    12121 tcttttggct taaatttaac gccattaggg tactttccgc tatatatatt ttctaacgtc
    12181 actttcatat acatatacgc tctccacgtt tctgcattca tcatgtgact gttttttttg
    12241 ctcttttcca taactcttaa ttaaacattt ccatttgttc agcgacgtta tatttcactc
    12301 tgttcagcct ccacgtcctg tttggttggc cgtatgctcc ctcacgtttg cctggcagtt
    12361 tctctaaatc ttccttacct gttgcagtaa gattgctgat tgctctgcgt atagaagtga
    12421 taggaacctt acggctaatc acaccctttt ttatcatgta ctcatacacc tcgcatggcg
    12481 taaagtcttt caatcggttg tccttgaata cctgaagtac gagaacgtcc tgtttttgtg
    12541 ccttttgttc ggctgctttt aactccggtt tttgcatacc tgttgtattg tggtatgaat
    12601 gcatactgct accgttttgt tgacttaata ctgctttcat tgttgctaat tttagtgttt
    12661 tataaatttt gtttcaatag catccgctaa ccacaaatca tgctcctcat ttactccgat
    12721 aatttctatc gaaaataatt cgtctttcag gtgcaatatt gcctgacgaa cttctgtgaa
    12781 atactctgca aattcagagg tatcgacctt ttctacgaat agaattttgt cgctttcggt
    12841 aaacatttcg tcaatggcat ccgtactacc gtctattaaa ttgcctatca gtttgacgtg
    12901 tttagtaata tcaaacccta tatctgtaaa ctgcagattt gatttatcga tattcggatt
    12961 gaataccggt gctccgtcga ttatagagaa tgctacttgt agcttcgctc ttaattcggg
    13021 tgccaggtgc agtaagatat gacgattatt gtccaaacta tctttctgcc ataccataac
    13081 taaacagtaa tttgatttcg ttgttgcttt taatccgtac atgatttttt atttaaagtt
    13141 agaaaaatat ttcgagtgtg cactcttgca cacccgaaat ttaatacttt ttagttcaac
    13201 tgtgataatt cagcaaccgt cttacgtcct ctctttttta tcggtgtatg tgtataggac
    13261 ttcatttgct tccactttat attctccggt tgccttatct ccgtttttgg ctgtgcatcc
    13321 ttcaaatcgt gcgctgtgtc cactttctcc gaataccttt ataaagtctt tgattttcat
    13381 gtcttaaata tttgtatgtg aaatttttaa aatactttcc ttttcttccc aatgttccaa
    13441 tattatttcc cattgcgtgt tgttgagtat gaaatttgct gataccagga acgcaaagtt
    13501 ttcatagtta tctccataaa gaaattgagt tgcatagatc ggatatatta tcttatagaa
    13561 aatatcctcc gtgctttgtg agtagtcaaa ttttttgatt ttcattttct taaatttaga
    13621 ttgttaaaaa ttccgttact gccttgcgga ttcgttgaaa tgtgttttgt ttttactgtt
    13681 cggttaatag ttttatcgca tcacgaataa accttgctac atcttcacca ccgattagat
    13741 agttcccgct tgaatcgtac atataagccg aaaattcgtt tgaagttctg ttgtttaatt
    13801 gaaaatcttt ctcattctca aaaatgaaat tgataatcgc ctgttttaat tcctttttca
    13861 taaaatagtt tttagtttgt tattaaattg cttgtgtagg ttgtgaatgt tccgtacttt
    13921 ttcaccattt cggcggtaaa atcttctaac ggcgtgctgg tgctgtatat ttccgttttt
    13981 agtccttgtg tagttaatag cacgatttta tatttttctg ctgtgttctg catcttgttt
    14041 agtttaatgt gatttgtaaa gcggttcttt ttataaagtc tgggttgtcg gttgcttgct
    14101 gataaaaagc agtatattct gccgtatcta taagaccaca agtgttatat tcacttttaa
    14161 aagtaggtcc atatacggtt ccttctttca catatccagc acttaaaaaa ccacaaccag
    14221 gaaataaatt aaaaaagttt tcaatatcag gtccggattg ataagagaaa acaacgccgt
    14281 taacgttttt aaaagatatt gcgtgctggt taatttccaa taacataaat aaccctttat
    14341 atttgccgct gaataacttt tttgccgttt ttttgatagt atgtgataac ataagtaatt
    14401 ttttagtgat ttgattaaaa tagtttggtt aattaaatag tttttcttct gtgtcgttat
    14461 aatggaatgt tcttaccatg ttatgcttta ccggaactgg attaaaattt aatgctacgg
    14521 ttaatccttc tttgtgtgtt attgcctttc ctgtttcttt ttccaacatt ttcgctatgt
    14581 tagctgcaat ttcacaaaat atggttttgg tagtttcatc ttctcttttt attaaagagt
    14641 gtattgttac tccattttta tacgtttctg cttcaatata gcatccttca ttttgtattt
    14701 tagctttaat tgagttgtag ttattcataa aatctttttt agtttgttag ttaatttttc
    14761 ctgctttatg ctgtggagtt aaaaattgat gtttataatt ctataaataa atttgtataa
    14821 catgggttaa atggactgtc ttgtctgttt atttcattaa agaatttact tagtccgttt
    14881 attcctccgg ctgcctttat tgttcctttt aaaatcttgt atgtaaattc ataatcattt
    14941 ataacactgt attctttacc acttggaaat attacttttt ctttttcaaa gtcaattttt
    15001 attattcctt ctcttaattt ctgcccgtgt atgttgtaag aaattaaatt tatttcttgt
    15061 gctgtggttg ctgttgctgt attcatttta ttatttttac tttgtgatta aatcgggttt
    15121 ataaataaag aatttaattg tttctgctgt aaagtttccc gctttcagaa agtcgttaaa
    15181 ttctgcgtta aactttgcgt taaattcatc taaaaattta gggttgtatt catttttact
    15241 ttgccatgtt ttaataaagt ctaaaacttt gttattttca ttaatggctg tttgtacttc
    15301 ttgtgatggt ggtgtttgca tggtgtttat tttttaaaat aattgtaatt gatttttatc
    15361 tgtttcctgt gttacttcga tttgtggcgt gtattcgctt atgcttcaaa tacttccttt
    15421 gttgtcatgt cgtttatttt tgttgttgtt ttaatttgat agtgtaaatc tatgcaggat
    15481 atttgctttg tgcaatatcc cacaccatta taatactatt attttcaaaa taaaaatgta
    15541 aatactctta tttcaatctg ttatgaactg ttactaaaat gttaaggaat ttaaagaaac
    15601 cattttcagg cttgaaaata ggctgatttt ttctctatgt agtttttatt aatatttctg
    15661 aatacgataa atctgcattg gtattcaggc tacatcttat atctctcttt tttttgagtg
    15721 gaggatataa aataaaaaaa cccgactata aaaatagtca ggctttttca ccaataaaca
    15781 cttcctgcgt attactgtac gttagtatcg cctacgctga aagttacggt tcgtataagg
    15841 ttatcggagt tatctccttt cgtttcataa ttagcggtct ccactttctt aatccacacg
    15901 ttctccaaaa tatgcgtctg caccggttgg ccgttcgcat ccaattcata cagaacaccg
    15961 ttaccgcaat actgcgaacg cttcatggtt tttgctttat tcagcttgtt ccataaatcg
    16021 gtatcacctg tatcgggaac taccatttct gcagtcatat ctcctacgat tttcttgccc
    16081 ggagttttcg tatcgggatc gttaccctgg gtaccctgct tatgctccgt ccattctacc
    16141 gacggcagtt gtacgttttg taaacgttcg tttgatacat cgtccaaatc taaacgccaa
    16201 ttaaagttgc cttgtggatt atttattgcc atgacttttg aattagttta ctgttaataa
    16261 ttgttgaatg ttttgaatag ttacgctatc tgcaggtgca atgtcgatag ctatgtattc
    16321 gtttgatgca atcggcttga atgcaaaacg aacacgatat ttacctgcat ctacgtcgct
    16381 ctttgtgttg aactgaagtt cattcaggtt cttagcgttt tggtcgccta accaatgcca
    16441 ccatgttccc tcaccgtatt tcgggccggt accaccctcg attgctctac cggcaatcaa
    16501 ctcgtcgata atgaacggac gcacctttct gtataatctt ccgaacataa ccaaatcatt
    16561 cggattgaaa gacatacgct ctgcaatcag ctttacctct cttgaaatat aaaccaccag
    16621 gtcggcaatg tttgttttgc tcaataaaga ggtacggttc aggttcaatg ttctgttacc
    16681 ccaattcgtg attttgaacg tcggatgatt tacgatagca ttcacaccac cctcatacaa
    16741 atcgtcgtaa taacctttgt atgcaggaga tttgaagtta atcgccacgt cattcacacc
    16801 gaataattta ccgaacattt caccggaatc agatatccaa acacctgctt tatcgtctgc
    16861 taatgttctg ttagctaatt ggtcgccaat accgcttatt acatatcctg tattagagga
    16921 atttgcagga tcgtttattt cgatgtctgt gtaccacata tctccgtaaa aagaatcaat
    16981 cggctgatga gagaatgagc cggtaccgtt tctgaaatca gtgatacctt caatgcttaa
    17041 tcctaacgga gtataaattc tgcagcgcat atcaccacga gccataacgt atgcaaccaa
    17101 tccctgatta gcagtatgtg taggctttga aacgttccaa atacgcatac tgtcactcac
    17161 gtcattgaat gagtgatatc cgtttttaga tattggacta ccgataaaat cagcatcatt
    17221 aatcaatgat acgtcctgtg taccgtctgc taaaataaac gtaccaatag gtaacatatt
    17281 acctacattc attgaactga agtcgaatgc tactacaccg atttgtccgt tcaaagatgc
    17341 acacgtttca gatgttagca ctctgtcata gttgctcact tgtattggta aatcgttatc
    17401 gggtaacgtt actttgatgt caactttacc cggctgattt gaaattgatt ttacgatttc
    17461 aattactgta ccattgtaac cggagcctac tgcttcagca tcaatttcca ccgatgcgtc
    17521 aacacctgta acaccacctg caaaacggcc attaccggct actgttcctg cagttgctcc
    17581 tgtctgtacc acgttcagga aatatctgtt cgcatcttct ccggaaccgg ttggtgcaat
    17641 caccgtaacg atgtaaccac cgtcgattgt agcagtataa ccatgcgtac ctgtacctgc
    17701 atttaccgca gtatataatg ccgtatttac agttgctacc gttggactac ctgatgtcgg
    17761 aattgtgtac gatgcaatca ctacctcacc acttactgta ttcactacat actgaaccac
    17821 aacgtcaccg ctcggaacgg tagtcaattt tgcatttgct ctcgctaata cttcagtaac
    17881 gttttgtgtg gccgtaactg ttgcaacgtc accctcaacg gtagttgcgt cgtcctgatc
    17941 tgtcaaatga aaaactctcg ctactcgcaa ctttgctcct ttgtttaagc ctcttgacaa
    18001 cttgtagaag tcttgattcc cgggaatcgc accacctaat attcttttca attcggtagc
    18061 attggtaatc aaatattgct tacctaactt tcctcttttg gcaatgatgt ttacacaaat
    18121 aattccttta ttgctatttg ccggtattac gcctacatct gatatgttgg tagatgctcc
    18181 gggagttcct gataagtttg tcattttcct gaattttaaa tttttagatt atcgttatac
    18241 tgtaatttat acaaatactt ttaaatttct aaatctatgt tgaataaatc ctcattacct
    18301 gcatttgtat tgttaggctc ggtgcctaaa gcgaaatcaa actgaactaa ttgtggtacc
    18361 gtatcaatat ttttccaacc aattaaatca atattaatag cttcaaacct ccactgtttc
    18421 tcaataaaat tagtacctga agtatcaaca aatccacgat tcataagcca aaaacctgca
    18481 acgttttcta ttgattcaat actaccacca aagtcgtcag ataggtcgtt attgtaatca
    18541 tctaaaacgt tcattattat atcaccattg ctccttaccg ctcttataaa ctttcttgat
    18601 tcaaatgcat tcagtataat atcctcaata atttcagcgt atttttcgct aattgtattg
    18661 tacgatatac tatacataat gtcatatcga gtgtcgggag tgtgaatttt atcaaacgtt
    18721 ccattatcat tttgaacgaa tgctatttct ttacctgtac caatctttgc agggacaacc
    18781 gatgctctat tgataataat gttattattc atatcctcac ccttgctctt ataggtaccg
    18841 acattgaata cttcaattac ttctttaccg gaactgataa ttgctccttt ggcagattga
    18901 tatgcactac tgctaccggc tgaaacaaag tcaggcagat aaccgccggc caccaccgct
    18961 ttgcgtatta cttcgaatat accgtcgctt atttctgatt gcttgaattt catattccgt
    19021 atttttttct gatataatcc atgagcatta cttcgtacaa tttctccgtt ctgattttca
    19081 tacccatcca cctcatggcc ggagttaaat atggtcgtgg aggaatgtta cgctttgcgc
    19141 tacctacttc cataatcaac gcaatgttcg ccacgctctc tccgtcattg tactttactt
    19201 cacgctttac tccgcagaag atcgtgtcca tacttgctac cgatgtgata ttattaatca
    19261 acgtacctgt acgcctcaat gtcagatttg aataaccctg tttctctttc cgctttttgt
    19321 acttctcgtt caatggtgcc cacaacccag gttggcgctt aatccacttt atgacgtacc
    19381 gctccaataa ggaacccatt ttcctgttca cgataaggtt actatcttta atgtcatgct
    19441 tcagattttt tgtaaccata ccggctaaat cccacctgcc aacttttttt atattcttag
    19501 ccatttggcc tgatattctt cttaaagtga actttcaata aacagttttt gtctttcagt
    19561 tgtcctaaca tattcaacga taaaatctca tgcttttcat tatcgaaaac gagataatct
    19621 ttcgcaggat ttacgatttg gttatgtgtg gaggaaacta cacctgcagt ttgtgcatct
    19681 tcgtaattca gcaatacata accgtcggct aaatcaatgg caccattcaa tcctttgctc
    19741 aacaatgctc cgttcccttg atcgttccaa acaaccaatc ccgacaatgc tatatcgtcg
    19801 tatgtacgct ctccgtcgat gtcacgcatg aacctggttg cagtattctt gtctttcttc
    19861 catacgatag gtaaacgcag gaaagtatcg gtaacgtctt taatcgtatc acgtatctca
    19921 tcaaattcgc tatctgtcag taatgatgcc ataattaata gtcgttttga ggattagaac
    19981 tactgccaca accgcagata ttcgacgtgc caccatacgc tatgaatgca gggccgtaac
    20041 ctttgctctt tataatgccg gagcataatg gtaggctaca tttcaacgta cttgcataac
    20101 tgcaaatctc ttttaacagg ccattcatca agtttgttgc ctccatagtc aatgtggtac
    20161 cgtcggatgc ttttgcatag tcaaattcgg cctcaacaac gtcagcttta ccttttttga
    20221 tacgcttgtt cccggacgga gcactacctc ctacaccacc aactacctca atgattttat
    20281 tgtagataag caggtagcaa acatatcgtg ccactaagaa cctccggagg ccggtatatt
    20341 tcgaatcgtc ctctacctct gcatctgtca attttgtgca atgctgaagt gcataataaa
    20401 cctctaattt cagagattct attcgagcgt catacgtgtt atcattaggt atgaaaggta
    20461 gtcgctcttt cactaattgt attactgttc ctattgccat aatactaaat ataaataaaa
    20521 aaaacggata ctgtgagcat ccgtttttct ttttctgtat gaataaatct tagtccaata
    20581 aggctttcaa ttcttccaac ttttctgccg gtaattccga taatgatttt gcgacatcat
    20641 ttttcttctt tccgatatac tcactttcga gtttgtaccc tttactgtct ttggttacga
    20701 taccctggtc gatgaacttc tctgccaaat ccacgaacgt ttcatctgca gttgtttctt
    20761 ctgttttttc cacttctgat ttttcgctta ccttgttatc ctcgatagct ttttcttcag
    20821 cagaaacttc aaccggattg ctctttaccc aagcgtcgta ttccgtttct tttgcctcca
    20881 cgataactcc gtctttcaaa agttttgaaa cgtaaacgtc tttcagaact ttctcaactt
    20941 ttttaccgaa caggtttttt tgctgatgca atatactgtg tgagttactt gcatctttca
    21001 actttacaaa aatttccata ctatttattt aatggtgaaa aatattactc ctcatggtat
    21061 ttgaacgcct cgttgatacg tgcgtcgata tccatgtatg cagggaaacc ttgtgcgctg
    21121 aacaatactg atttgtcaat gattaaacgt gcatcacgtc tgataattga aaatccgata
    21181 tagtcgctta caaagatttc gttctcctgt gtttgtggat ttcttctttc ctccattttc
    21241 atagaaccgt actgcaattt cgccattgct tttgtactgt ctaacaacat gatctgattg
    21301 cttccaactg tgtacacgtc gtttttcaat gtatcaggca ctcctaagat tgactgaata
    21361 gttgccattt tgttgccacc attgaaaccc tgaaactccg gtaatgaagt gatatcgata
    21421 ccgtcgtttt cacctgtaat gattgtatca ggcattcttt tcaatcgacg taaacgggac
    21481 gtaccacgtt tgatgtcttt gaagttgaat gtgttggcag tcgcaacacc gatagtcgga
    21541 gcggattctg cacctgaaga ttgctcaccg ttcaccaata ctctgtaagc ctcatagtca
    21601 gcgcagattg acatatctac acccacctca cctaagaacg tgaaaatcat gtccaaagat
    21661 gaacgctcaa tcagttcgtc tgtgattttg aaacccaaac caactttgaa tacaccggct
    21721 ttctttttac cgaatgatac ggaaccaaat tcgatacttt caccctcacc aactttacgt
    21781 gcaaccgcat tacctttctt gatgaacggc attgtgattt cgtcctgtga gattgattgc
    21841 gtcgtggcaa cccaaccctg gtgcaatgaa cttgcatcgt aatccaaacg gatagccgtt
    21901 aagattaatt caggaatgat gaaacggtag tctgcaggaa caccggaggt attcgctaat
    21961 gcatcgaact gtgaatattt agtaagtgca tctgtcaaag actgaacacc taaattcgca
    22021 ttacccaaac gagcggccgc agttttcaga gtatcggaac ccatgaaaat atccatttgc
    22081 ttcaggaact ccttaacaac atgcttttgt tgttgcttgt agcttaactt ttcgccactt
    22141 tcggtttgct ttgccggctg aataccatag tggatgctga tagcatctgc aaacgaaata
    22201 ttgtatgccg ggataacacg accgtaagca tcggttgctc taccttgtct agttaacgca
    22261 atttcttttg caaaatccgg gatcgtttct tcggttaacg tctttacttc atctgcttga
    22321 agtctgtcag ttttatcacc tttgaatact gctaagttgt gttttctcaa ctttaatagg
    22381 ttttctttca ttttatttga tttatacgat tactaattga ttacgattaa cgattatgct
    22441 ccaggcgtta aatacacacc ctgaatgata ccgatacgaa ctcttgcatc ttcaactgct
    22501 gctttcaatg tgaatgctga cacataggct gatgcaccgg ctgctacata gttcggatat
    22561 ccgtctgcat ttaatgtacc gtcaggaact actgcaacac ctgcatttaa agcaccgcct
    22621 ttcgcaatac cctcgataac catttgatga ggcacacgca ctaatacacg ctctccgtcc
    22681 ggagtatcag ataatgcaat tcccaaagga atttgcgtac ctaatgtacg ttttttaacg
    22741 gtaccgtcag tatgttgata tacctcctgt cctgctttta tttcgctacc tgctgaactt
    22801 acaaaagtta gttgtaacgc atctgaagtc atgtttttta tgacttgtgt aggggttgtt
    22861 tggatagaac ctgtcatctt ttttgatttt tataatttga taaaataaat aattaatttc
    22921 tgatttgttc tgccatagaa acgaatgacg ttttctctgt ttcctctttc tgctcaccct
    22981 cgttcactga agaacggaaa ctcactttag tagaattaca ttcgtcacat tttccaccga
    23041 actgctccat gcaggtaccg ccatactgtt gcatcaatcc ctccaatgca tcgaaactcg
    23101 cttcattgat tgtcttaatg actgcatcat tggttttgtc ttttacagaa accttgtata
    23161 aacggatgca ctcctcacgc ttggataatt caaccttacg tccgaactct gccaaaggtt
    23221 ttacttgtgc caggtcgata acttttgcaa actcggaaag ttcagctacc ggaacgattt
    23281 tctcgtattc agcaaatgca tttttcgctt cagttaattc gctttgaact ttagccaaag
    23341 attcttctgc agtagtcttt gcagatttga actcctccac ctcttttgtc aggtttgtga
    23401 ttgtttctgt ttcgtctttc aactcaacaa attcaacttt gaaagattct tctaaggtag
    23461 taagcacttc ctgtgcgtaa tcttcttttt tgccggataa ttttaatact ttcatttcgt
    23521 tatttttggg tgaatttcca ttttttgaaa aatcgacgtt aattcgttct cttaaattta
    23581 tacttttttc gtttgcaaag gtaacatcgt ccattaaata gtaatggccg ttacttcttt
    23641 tgtaataatc gctaccaatt ttatcatccg aaaacatctg tctgccaacg attgcagatt
    23701 tctctacatt caccaacttg ccctcggtat ctactatttt tgcaaacgga tctgctccta
    23761 aaaataccaa tgatgtttca tagaagtcca caattttagt aaccacacgt cttaccattg
    23821 taccgtcaac cagggtaccg atacgcattt cgaacatcca atcatcctcg ttaccgcttt
    23881 ggtccgtaaa cgtatgtgac ggctcccact cgaataagag agatactgat accgattgga
    23941 tatgtggcac cgggaatgcc gataatttac ggcatatatc cgtatgtaac ttgccgtcaa
    24001 tccaaatagg tgcattcagt cctgcaggaa taatggtgcc gtcatttgcc ttgtatgcat
    24061 tctcataaac cactttacca ttaacaccaa ttatattacc tgtttccgca ttgtgattta
    24121 ctaatgcagg tttatattcg agcaatgatt tagcctcttt caaaactttc tcattaaact
    24181 cggtagcttt ccaactccat gctcctacaa tggttgcaga tatgtgacgg aaattgaacg
    24241 ctaaaaaatc ctcctttttt gcaatcgcat tggagatatc gggatttacg actgactgcc
    24301 tgtagtcctc attatcccaa ctaaatgact gtttcttacc gctatcaagt tcaataaatt
    24361 tgggtgccaa cagtaaacca ttggtattat taaacattgt aattttccct ttcttagaca
    24421 taagtaactt tactttatct ctaagtataa tgaaaaaatc gttataaatc aaaacggcat
    24481 ag
//
LOCUS       DAOOOL010000023        24442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_159_length_24442_cov_102.652807, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000023 DAOOOL010000000
VERSION     DAOOOL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24442
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159_length_24442_cov_102.652807"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKU82_03410"
     CDS             <1..678
                     /locus_tag="PKU82_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81952.1"
                     /translation="MADAAGLDRQINTFDTNGVMTNSVTFFGNNNYNVSGSSVPLVLH
                     VENDDEIYIVSQTGGTSGMWYISSLTTTPAPTISQNFTSLSANYNLTGTTYQWYLNGN
                     IINGATDSTYTFTQNGVYSVTVTIASGCDYSYSYTVTNTGIANAANLNNIKVSPNPFN
                     DYINISNTTGQLTTLTLFDAAGRTVILETTHEPDYKKQLQQLPKGMYFLTIESGSQSK
                     HIKLLKH"
     gene            797..3406
                     /locus_tag="PKU82_03415"
     CDS             797..3406
                     /locus_tag="PKU82_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81953.1"
                     /translation="MHVKIFSVLICIVLLNTNSAFAQGVGINNPTPDATALLDLTSTT
                     KGLLIPRMMTFQRLSIPAPANGLMVYDITLNAFYYSDNGIWKPMDNSWQLNGNTATNP
                     VTDFIGTTDFTPLVFRTNGFEAMRITQDGRIGINQPFSPAFNAILDIQSNDKGILIPR
                     MSSPLRSNISSPPDGLLVYDSGSNEFYYSISSVWMRLLSNNTGWTTRGNAFTNPATDF
                     IGTTDNTDLVFGTFGGARGCITSAGDWGIGTIFPSAKLHVQGSYRLEDGTQGLGKLLM
                     SDATGNASWKTFEDLITGKVANGLYFNTIQQRILIGGALTENTTITNSAFDFTYDLTG
                     AGDFQVTESSNPIPSLFVSGNKGGVNDGYVGIGTATPFGRFHINNTVVTTVNTQNTYH
                     LLIGNGNTNPDLTFGASATFALAQSWNNKPLQINPLGTFVGINHISAAPIQNLDINGR
                     LAVQNGVIQKGAATAILATDLGLYQQIAGGTIRISNNAGPIKFYTDQGGASLGGTNNL
                     VSIDNSNGGGVKIHSEISGSGQALVPAARAALDVQSTTKGVLFPRMTTAQRNAILPLT
                     AADEGLMIYNLTEHCINWWDTRPNSAGVPGFWNSQCQPCMDYFEYTASSNGNNFNTQA
                     GSPVGPKTFCVTVKAGVTLGASAMSGDALNFGALPAGSKVILHNYGQIRGGGGKGGNG
                     GSERDVTVCGVDAPATVGSDGGDAIVTSAGVQVTVYNYGTIASGGGGGGGGSGGCRSN
                     GGSGGGGAGIPAGGGGLSPNTGGRASGGLCNVCTLAPASLAGANGTITNGGLGTCSQG
                     NAGGGCSSLGFNGGCGGNGGGPGVNGNTGVGLSCGTAIAAAGGNAGFALQGNGSGSFI
                     VNVTGTFLGIVSP"
     gene            3551..5089
                     /locus_tag="PKU82_03420"
     CDS             3551..5089
                     /locus_tag="PKU82_03420"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81954.1"
                     /translation="MKKKKYNTKLNLTCILISMLMVILVFNESPAQLLTNNNVLISNS
                     IQITVKGTVQNNFGTVISNSGTIDLTGNWVNNSGNNGFGISQGTVIMNGVNQSIQGTD
                     QTVFNNLSLINGTKTIDLNTTVGGANATPTGVLNCNNAVLDLNSNTLTVNNPIGTAIT
                     TSTGYILSEDVDHSSKVTWKIASTTGIHSIPFGNAGGVQIPYSFNLTTGVAGEVTVST
                     YATVPVNTPYPVSPTMVTHVRDASGADNSANTVDRFWQVDKTGNGTAAYTFTYAATEN
                     AAGGNINIRAQSWNTTNLGWDAALPGQLNPTVQSVLVPGVNTNGAWALALASNPLPIE
                     LLSFTAKSKDNKKVVCNWSTASEINNDFFTVFRSKDGINFEEVSKVDGSGTTNTQHNY
                     SFTDNNPYKGVSYYRLKQTDFNGDYTFSQIENVLITDGDIKYLVYPNPNNGNFSVKFD
                     AEQNVNAVFLITDATGRLIQSGRLDDLSGKQQINIEKVLPGFYFLQITNGDNSYLAKV
                     QVIK"
     gene            complement(5855..7399)
                     /locus_tag="PKU82_03425"
     CDS             complement(5855..7399)
                     /locus_tag="PKU82_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81955.1"
                     /translation="MKNYVTVFALLILSVKVNSQTVQPNIPTANDRVNCIVRYNNTIY
                     IGGEFDTVGTSARPYLAAIDANTGAILPWNPVPNAQVTKMIICNSKLVVLGDFSSISG
                     SLIDRIAVYDLQSGNLLNLNSIPLNLIGIGRYGLYNAGNFVYFTASDIFGSVDAVRRF
                     DLNTLLLDTIWVSSNFNVNKCELISSGNYIYAAGWFNHYLTNSTIKNLCRFDMLTGII
                     DTTFSFSFSNSDYLNQIAAQNGKLYVSGNFSAIDGLTRKGIAEIDTSGFVTSKNIYCS
                     NSLIDALTLQGNTLWVGGNSVVLGGLNRYCIAQIDISTGIATCWFTQALQGNVYMTSI
                     WASHDTIYSAPNIEGFKTFTGNPGFVNIGNDSVLCAGSSLMLNAPNGLTNYLWNTGAT
                     TSSITVNAPGSYWFSATGSGGCIVTGYRILSACTGIENQISLVPIQILPTISNGIYSI
                     QFPLQKVSGIIKLHDSSGNLVRTFSTSPLENAKQLNISSLASGIYFCSVVWESKISIV
                     LKLVKE"
     gene            complement(7715..8197)
                     /locus_tag="PKU82_03430"
     CDS             complement(7715..8197)
                     /locus_tag="PKU82_03430"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOZ81956.1"
                     /translation="MNKQVDTSKGPGVYIPPPLFYVLIFVAAVYIQKKVPILDSALHT
                     LVIKSLGIILFITALFFLFRSLRQFFITKNTIILIKPASSLQTTGIFAITRNPMYVGL
                     AIVYLGVTIFIGNWWNIILFPILILIVQEYIIKREESYLAIEFEQEYENYKKKVRRWL
                     "
     gene            complement(8233..8949)
                     /locus_tag="PKU82_03435"
     CDS             complement(8233..8949)
                     /locus_tag="PKU82_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOZ81957.1"
                     /translation="MPLSYISNKVISKGEIVKGQEIREGIFLLIKSDFPNFDANSIIS
                     IDELNKYRRLYLTKLVEQEKGEIATIDLDVMQAIRDNSILSENIQDEIEAKLTMGQKL
                     ADKIASFGGSWTFILSFFFFLLLWIIINIWLLKSKPFDPYPFILLNLILSCLAAIQAP
                     IIMMSQNRQEQKDRQRGEHDYKINLKAELEIKLLSEKIDHLLVHQNKKLLEIQEVQID
                     YLEDLMKQLKINYVNIKKQE"
     gene            complement(9047..10378)
                     /locus_tag="PKU82_03440"
     CDS             complement(9047..10378)
                     /locus_tag="PKU82_03440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOZ81958.1"
                     /translation="MTKRIVKIQGFIIVFLTVVLPAFSQSNPNPSTSVDAFIQSEMNT
                     QHFPGVSTVIVKDDKIVWINSYGFADVANTIPVSDTTVFLMASVSKVFTGTAAMQLYE
                     NNVINLDGDVNQYLPWLLQIPGYPNDTISFRQLMTHTSSIQDNWAAMVGYYGYPDPTI
                     TLDDCMQRYFSTSGSDYNASANFLATAPGSVFQYSNIATALNGYLVERASGIPFDQYC
                     NINIFNKLCMNKTRWYYSNFNPNDVAIPYHYSGNNYVAYPQYGFADYPDGQLRSNVTD
                     LANFMIAYLNGGTFGTNSILSPASINQMWTVQFPSLNTRQGLNWYKEVLYYNGGSAWV
                     WGHNGGESGVSTEIYLDPVNKIGLCVLSNGEGNGLNICDQLYNYALSLNASSGYSPAC
                     LTTGIAETTTDIKRKVIKIIDLLGRESPLKTNTPLIKIFSDGSAEKFFIID"
     gene            complement(10455..12038)
                     /locus_tag="PKU82_03445"
     CDS             complement(10455..12038)
                     /locus_tag="PKU82_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ81959.1"
                     /translation="MKKLLLVIANFCFVSLAEAQVKFQKVYGSVSDEEAYSVNLTSDG
                     GYIIGGYTSNLGNGGKDALVLKIDATGNMQWTKSFGSTNDDYIMDIKQTFSGEYYACG
                     IYDDNGNGNENIFCCKLNASGDIQWFNSFNINYTVPNKNWRIHLKQAIDSTLLLASVG
                     GTAGSNTVLAKFSVAGTMLWQSSFGGYLYGSDVAELSNGDLILIGSVGGFGAGLIDDY
                     IMKTSSSGTLIWSKAIGEINNDGSYSLNILPNDGFITTGNTWNNAIGQSDAKIIKYDS
                     DGNLIWSKNIGESGRRFISQSGCLTHDNGFAAVICDVAPSSASKMTIVKIDSTGNILW
                     SKNYGGADEDNSFAVKNTTDNGFIIAGYATSFGVGGKDIYIVKTDSIGNSGCSTTQFT
                     NTPSSANWPGIVNIFSPTIGSPFPILNQIMVSTPSIAADVFICGDTTVSIKENSNLIY
                     QLSPNPTNGQFTISFDKLIPTGKVNIYNAVGEIVGCEVINNESQKLINLRNISAGVYF
                     VNVLNGEASYRKKLIVTQE"
     gene            12265..12603
                     /gene="ytxJ"
                     /locus_tag="PKU82_03450"
     CDS             12265..12603
                     /gene="ytxJ"
                     /locus_tag="PKU82_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HOZ81960.1"
                     /translation="MGFKTLDNIAQLNEIDTLSKNKKQIIFKHSTRCSVSTFAKKVLL
                     SEFSDDMEKKFDVYYLDLLNYREVSNKIANHYGVYHESPQILIISDGKCIYDASHDDV
                     SLASALASVN"
     gene            complement(12611..13945)
                     /locus_tag="PKU82_03455"
     CDS             complement(12611..13945)
                     /locus_tag="PKU82_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ81961.1"
                     /translation="MPSKILLVDDEPYIEDLVRQKFRKQIKENQFEFEYVSNGKIALE
                     KLKNDDSYDLMLTDINMPVMDGLTLLEKLRENNILTKAVVVSAYADLSNIRTAMNRGA
                     FDFITKPIDLADLEATIKKTVLEMEMLRKGIEASRNLDDALLQKAEAQQKALDALLEK
                     EKLILQQNELLETQVQQRTAELQQQKLLVEEKNKEILDSIHYASYLQRAILPSEETLA
                     SFFKESFVLYQPKDIVAGDFYWIEPVEDGHIVIVADCTGHGVAGALLSMLGISLLNHL
                     VRDKNITRPAELLNQLRIEVSRSLNQAETHAAEGMDIAVCHINCSAKQITFAGAYRPL
                     VFISGNELKTLSGNKIVIGGNNILQQQEFINQTISFDDAGTVYLFTDGYADQFGGEKN
                     KKLMSGKLKELLLEKHNLPMKEQLTFFTDYLKKWRGKNDQVDDVLLLGFRCQ"
     gene            complement(13949..16192)
                     /locus_tag="PKU82_03460"
     CDS             complement(13949..16192)
                     /locus_tag="PKU82_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOZ81962.1"
                     /translation="MFNLHRLFLSIFFSGICFNVIGQNSNKKSDLDSNVNIKVNLGNL
                     LSDDEDAQANADSTIQVFERNENLLEFTNNWKFKRGDDTLWKEPIYDDSVWEEYSDTI
                     RSNHIKNYTTIVWYRMHFDIDSSLLNRPLALILHLYGSAAEVYLDGRLLKKYGTVASN
                     ISEEEAVFNTNPQPVAVVFSNLNNHVLAIRYSNFHNSSARISGFNIGENFTIKFKDLN
                     SEIDDSIQTIKKSSLILFVAAVFLTLAIVHLIMFLYYRQKITNLYYSLYCIGIFIFTF
                     YTYHLLTSSNYSSIAFISKSLQFLAPLIVVPIVAMLHTIFYHHFRRNLWVLLGLYAVM
                     IVLYLLPFRKIAAILMAVIIITSFVEIFRVIIVSVRKKRDGAWIFALTLLLPVFLGII
                     ISILPDTITFWGMKFEVKTSNFISVSMLMGLPFSVALYLARDFARMGNTLTKQIDEIT
                     ELSTRTIRQEQEKKHLLENQKAMLESEVKERTHEVLEQKNVIEIKNREVTESLHYAKR
                     IQAAILPDIKKINQSLNDSFILYLPKDIVSGDFYSFAQRDEKIIVAAADCTGHGVAGA
                     FMSMIGTALLNQIVNEKGITEPSKILTQLNDGIVTSLKQRESEMNEGMDIALCTINNK
                     NKTLQYSGANRPLWLIRKNEFQFYKPNKIPIGGQQYNKADGFMQHNITLQQGDAIYLF
                     SDGFVDQFGGEFGKKFMTKKFRELLLSIQHLSMNEQKEYLLNTLIKWQGKHDQVDDIL
                     VIGIKIP"
     gene            complement(16263..17609)
                     /locus_tag="PKU82_03465"
     CDS             complement(16263..17609)
                     /locus_tag="PKU82_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ81963.1"
                     /translation="MTTMKKISIIGAGLVGSLLALYLNKRGYKVTLHERRPDMRKVKI
                     PAGRSINLALSDRGWRGLQGVGIDDAIRKIGIPMTHRTMHSVDGKITTQPYGKDGQGI
                     YAVSRGILNCTLLDLAEKGGVEIHFNQRCTGLTLASGEMHFENSESHQKTTFASDLIF
                     GADGAFAASRLQLQLSTEQFNYSQSYLQHGYKELMFVAKADGSHQLEKNALHIWPRGG
                     YMLIALPNLDGTFTVTLFFPLKGNPSFESLHNKQEVKTFFQKVFPDAVPLLHDLEEDF
                     FHNSTGSLVTVKCAPWSFDNKVVLIGDAAHAIVPFYGQGMNCGFEDCSVLNDLLNQYA
                     DDWQKVIPEYERMRKPAADAIGELAVDNFIEMRDLVGKPEFLLRKKIEGWFSTKHPDK
                     WIPLYTMVTFSHIPYHEAQARGKRQDAIMNEIMKMADIENIWNSSVVEKKIIDLLK"
     gene            complement(17606..18892)
                     /gene="kynU"
                     /locus_tag="PKU82_03470"
     CDS             complement(17606..18892)
                     /gene="kynU"
                     /locus_tag="PKU82_03470"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HOZ81964.1"
                     /translation="MHFINSSDFANQLDEQDPLKSFRQKFLFPQHQNKDVIYFCGNSL
                     GLQPKQTRDYVLQELNDWKDFGVEGHFQAKNPWYPYHEFLRDNMALIAGALPSETVVM
                     NSLTANIHFMLVSFYKPTTKRFKIICEGSAFPSDQYALQTQVKFHGFNPDNAIIELHP
                     RTGEHTIRHEDILETISKHKDELVLVFLGNPNYYTGQVFDMAGITQAGHAAGAIVGFD
                     LAHGIGNLELHLHDWNVDFAVWCSYKYLNAGPGGVAGAYVHQQHCNNTDLIRFAGWWG
                     NDPQTRFTMPHDFIPVKSADSWQLSNAPVLPMAALRASLEIFAEAGVQNLIKKRKLMN
                     DFLRFIIGDIISKTNSEKNIIMITPENENERAAQVSMLCKHNGKKIHGYLTEQGIIAD
                     WREPEVLRIAPVPLYNSFNDIYQFGKCLHEALLLNR"
     gene            complement(18967..19530)
                     /gene="gmk"
                     /locus_tag="PKU82_03475"
     CDS             complement(18967..19530)
                     /gene="gmk"
                     /locus_tag="PKU82_03475"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOZ81965.1"
                     /translation="MTKHFLFCGPSGSGKTSIVQHLLTKFDFMQFSISATTRPKRANE
                     TDGKDYYFMSIENFRKHIDRNDFVEWEQVYEGRYYGTLKSEVERIEALNCSVVFDVDV
                     EGGLKIKKHFGKNLLAVFIRPPSVEDLRQRLTARNTETAESLEIRLNKSIKELSYQDR
                     FDNVIINGELNQSCKEAEALVSAFLQN"
     gene            complement(19527..20399)
                     /locus_tag="PKU82_03480"
     CDS             complement(19527..20399)
                     /locus_tag="PKU82_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOZ81966.1"
                     /translation="MIRSMTGYGKASGDYKSKTISVEIKSLNSKFFDLNMRLPHPYKD
                     KEMDLRSLLLKEAERGKMDVMITMESDLAQQENLLNREIIKSLLTDLKAVKKEFALPD
                     DNLLQIVMQMPQVVNTDSDDADENEWKYIFSIINQAIEKFNLFRQREGHNLQQDISQR
                     MNLILQLLEQITPFEEGRINAVRSKLISSLKSLGGNIETDKNRFEQELIYYLEKLDIT
                     EEKVRLAEHCRYFLSMLNDENNNGKKLGFIMQEIGREINTIGSKANDADIQRKVVEMK
                     DEAEKIKEQLSNIL"
     gene            complement(20446..21354)
                     /locus_tag="PKU82_03485"
     CDS             complement(20446..21354)
                     /locus_tag="PKU82_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ81967.1"
                     /translation="MKATTRAHLSVLFVNLLYAANFSIAKQVMPEFIKPFGFIVIRVI
                     PATVLFFLAAFLIPSEKIKKKDRNTILLCALFGVAINQLLFFKGLSLTSPINGALIMT
                     TNPVLVLLMAAIILKEPVGWIKYIGITLGIAGAMLLILLGKNFNLQGGNTEGDLYIFL
                     NSVSFAIFIILVKPLMLHYHPITVMKWTFFFGALMVLPFGFNEVTAVAWSSLTFNLWL
                     CILFVIVAVTFVAYLMNVFALRHLSPSVVSIYIYFQPVFATLISMALGEASPGWLHLA
                     SAVLIFTGVYLVSKQKIAGINNTENS"
     gene            complement(21358..21735)
                     /locus_tag="PKU82_03490"
     CDS             complement(21358..21735)
                     /locus_tag="PKU82_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOZ81968.1"
                     /translation="MKKIYHLANCTTCQRILTELKPGKDFILQDIKSEPITEQQLDAM
                     QKLAGSYEALFSRVAMKYRSMGLNQIKLSEKDYKKYILEEYTFLKRPVIIIDKEIFIG
                     NAPKTVAAAKAAMHKNKNQTKNH"
     gene            complement(21782..23257)
                     /locus_tag="PKU82_03495"
     CDS             complement(21782..23257)
                     /locus_tag="PKU82_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ81969.1"
                     /translation="MRILLTGANGYIGMRLLPVLVEAGHDVTCVVRDALRFKPSPDLL
                     DKIHVIEYDFLEPENAIQHFNNKQFDVAYYLIHSLGDTATTLKEYELRCAGCFVLVAT
                     LTQVKQIIYLSGISNEDNLSKHLTARKGVKDVFINSGIAYTIFEAGIIVGSGSISFEI
                     IRGLTEKIPVMIVPRWLKSKCQPIAIRNVMNYLHLSLMNEKAFNRVFEIGGPDVLTYR
                     KMLLEYAAERGYKRLIITLPFGFMGLSATWIYFSTPANYTIAKLLVQSMKNDVVCKET
                     SVRDVIPQELYTYREAIQMAFARIEQNMVISSWTDSASSSLSRLDVNQHIEVPSNGCY
                     KDRKWVEIDKDKADEVANRFFNIGGQNGWYYADTLWRMRGIIDKLIGGVGMSRGRRSD
                     VDIEPGDTLDFWRVLLVDRKNHRLLLYAEMKLPGEAWLEFSIIHNNNRCILKQTATFR
                     PIGIMGRNYWYSMLPFHFFIFRNMIKQIAGDGQHKIRNTKI"
     gene            23422..>24442
                     /locus_tag="PKU82_03500"
     CDS             23422..>24442
                     /locus_tag="PKU82_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOZ81970.1"
                     /translation="MAKRTAKQNTRLKFDTAWQYSAAPETPDHIVLEDKYNLFINGKF
                     VAPQSKKYFDTINPAKETRIASVAEASQADVDAAVKAARTAYDKVWSKMPARERGKYI
                     YRIARIMQERARELAIIESLDGGKPIRESRDIDVPLAAAHFFYYAGWADKLDYALPGM
                     AAQPLGVAGQIIPWNFPILMAAWKIAPALACGNTVVLKPAETTPLTALKLAEIFRDAD
                     LPPGVVNIVTGAGATGAAIVNHPGIDKVAFTGSTDVGKIIQKALAGTTKKYTLELGGK
                     AANIIFEDAAIDQAVEGIVNGIFFNQGHVCCAGSRLFVQESVANEVVRKLKDRMETLI
                     IGDPLD"
BASE COUNT         7565 a         4678 c         4488 g         7711 t
ORIGIN      
        1 atggccgatg ccgcagggct tgaccgtcaa ataaacactt ttgatacaaa cggggtaatg
       61 accaacagcg ttactttttt tggaaacaat aattataatg tatcaggcag cagtgtgcca
      121 ttggtattgc atgttgaaaa cgatgatgaa atatacatag taagccaaac cggtggtact
      181 agtggcatgt ggtatatttc aagcctaacc acaacccctg ctccaactat ttcacaaaac
      241 tttacatcgc taagtgcaaa ttacaattta acaggcacta cttaccaatg gtatttgaat
      301 ggcaatatta ttaatggcgc aacggatagc acttatacat ttacacaaaa cggggtttat
      361 agtgttacag taaccattgc ctcaggctgc gattattcgt attcatacac ggtaaccaac
      421 accggaattg ctaatgctgc taaccttaac aacattaagg tttcgcccaa tccgtttaac
      481 gactatataa acattagcaa tacaaccggc cagcttacaa cccttacttt atttgatgcc
      541 gcaggccgta cagtaatttt agaaaccacc catgagcctg attataaaaa gcaactacaa
      601 caactgccaa aaggcatgta ttttctaacg attgaatccg gcagccaatc caagcatatc
      661 aagcttctga aacactaaat acaaccctgc aacagcgcac ggccgcaagt ccgtgcgctg
      721 ttttaatatc cccgaattat gggattgacc aagcctacct cccaaaatat ttttgttacc
      781 tcaactgccg gcaattatgc atgtaaaaat atttagtgta cttatatgta ttgtactctt
      841 aaacacaaac agtgcctttg cacaaggcgt gggaattaac aatccaacgc ccgatgccac
      901 agccctgctt gatttaacca gcactaccaa aggtttgctc ataccacgga tgatgacttt
      961 tcagcggcta tccatacccg cacctgccaa tggcttaatg gtttacgaca ttacattgaa
     1021 tgctttctat tattcggaca atggcatttg gaaacccatg gataatagct ggcagctaaa
     1081 tgggaataca gctaccaatc ctgttactga ctttattgga acaacggatt ttacgccttt
     1141 ggtgtttcgt actaacggtt ttgaggcgat gcggattaca caagatggca gaattggcat
     1201 caaccagcca ttttctccag catttaatgc cattcttgat atacaaagta acgataaagg
     1261 catacttata cctcgcatgt cttcacccct gcgcagtaat atttcttctc ctcctgacgg
     1321 cctattggta tatgacagtg gttcaaatga attttattat tctatatctt ctgtttggat
     1381 gagattgctt tctaacaata cggggtggac cactagagga aatgctttca ccaatccagc
     1441 cactgatttt ataggtacaa cagataatac cgatttagtg tttggtacat ttggtggagc
     1501 acgagggtgt ataacttcag ccggtgattg gggcataggt accatttttc cttctgcaaa
     1561 gctgcatgtg cagggcagtt accggttaga agatgggaca caaggcctgg gaaaattatt
     1621 aatgagtgat gctacaggca atgcaagctg gaaaacattt gaagacctta ttaccggcaa
     1681 ggtagctaat gggctttact ttaatactat acaacaaagg atacttattg gcggtgcatt
     1741 aaccgaaaac accactatta caaattccgc ttttgatttt acttatgacc ttaccggtgc
     1801 cggtgacttt caggtaacag aaagttccaa tccaatacct tctctttttg tttcaggcaa
     1861 caagggaggc gttaacgatg gatatgtagg cataggtact gccacaccgt ttggcaggtt
     1921 tcatatcaat aatacagtgg ttaccacagt aaacacacaa aacacctatc acttattaat
     1981 tgggaacggt aatacaaacc ctgatttaac ttttggagca agtgcaacat ttgcactcgc
     2041 acaaagttgg aacaataaac ccctacaaat taacccacta ggcacctttg ttggcataaa
     2101 tcatatttca gcagcaccca tacaaaactt agacatcaat ggccggcttg ccgtgcaaaa
     2161 cggagttatt caaaaaggcg cagcaacagc aatattggct actgacttgg gtttgtacca
     2221 gcaaatagct gggggcacca tacgcatatc caataatgcc ggccccataa agttttatac
     2281 cgaccagggt ggtgcaagtt tgggtggcac caataacctg gtaagcattg acaacagtaa
     2341 tggcggaggg gtaaaaatcc acagcgaaat tagtggatcc gggcaagccc tggtacctgc
     2401 agcacgtgcg gctttagatg tacaaagtac caccaaaggt gttttatttc cacgcatgac
     2461 tacggcacag cgcaatgcca tactgccgct taccgcagcc gatgaaggct taatgattta
     2521 caatttaacg gagcattgca taaactggtg ggacacccgc cctaacagtg ccggtgtccc
     2581 cggcttttgg aacagccaat gccagccttg tatggattac tttgaataca ctgcaagttc
     2641 taacggcaac aattttaata cgcaagcagg ttcgcccgtt gggcctaaaa cattttgtgt
     2701 tacggttaag gcaggtgtta ccttgggcgc aagcgctatg agtggcgatg ctttaaactt
     2761 tggcgcttta cctgcaggtt ccaaagtaat attacacaac tacggccaaa tacgtggagg
     2821 tggtggcaaa ggcggcaatg gtggctcaga aagggatgtt actgtttgcg gggttgatgc
     2881 accggcaaca gtagggtccg atggtgggga tgctattgtt acttcggccg gggtgcaggt
     2941 tacggtttat aactacggca ccattgcctc aggcggtggc ggtggcggtg gcggcagcgg
     3001 agggtgccgc agcaatggtg gcagtggtgg gggtggtgcc ggcataccag ccggtggtgg
     3061 tgggttaagt cctaacacag gtggcagagc atccggtggc ctttgcaatg tatgtactct
     3121 tgcgcctgct tcattggctg gtgctaatgg aaccataacc aatggtggcc ttggcacttg
     3181 ctcacaagga aatgcaggtg gtggttgctc ttcgcttggt tttaatggag gttgtggtgg
     3241 caatggtggc ggccccggtg ttaatggaaa taccggagtg ggcctttcct gcggcacagc
     3301 catagccgca gccggtggca atgccggctt tgcattacaa ggcaatggca gtggcagttt
     3361 tattgtaaat gttacaggga catttttggg tatcgtatct ccataattac tcacacttaa
     3421 gtagtatttt tttcatcctt aaaccatttg atatttgcct gtaacaaaaa tttaccgttg
     3481 gataaaataa catccactta ataaattaaa ctggcttaac ccttttgtca gcctttaaat
     3541 tgatgcataa atgaaaaaga aaaaatataa caccaagttg aatctaacat gcattctcat
     3601 ttccatgttg atggtgattt tggttttcaa tgaatcacct gctcagcttt taaccaataa
     3661 taatgtttta atttccaact caatacagat tacagttaag ggtactgtgc aaaataattt
     3721 tggaacggtg atttcaaatt cgggcacaat tgatttaact ggaaactggg taaataattc
     3781 aggaaacaat ggatttggga tttcgcaggg aaccgtcatt atgaacggag taaatcagtc
     3841 tatacaagga actgaccaaa cggtgtttaa taatcttagc ctaattaatg gaacaaaaac
     3901 aattgattta aatacaaccg ttggtggtgc aaatgcaaca cccacaggag tattaaactg
     3961 taacaatgct gttctcgatt taaattcaaa cacgctaacg gtaaacaatc ctattggcac
     4021 ggcaattacc acttccacag gttatattct tagtgaagat gttgaccaca gtagtaaagt
     4081 tacctggaaa attgcaagca ctacaggtat tcattccatc ccatttggta atgcaggtgg
     4141 agtgcagatt ccttattcat ttaatctcac aactggggtt gcaggtgaag ttactgtttc
     4201 aacgtatgct actgttcctg tgaatactcc ctacccagtg tcaccaacaa tggtgactca
     4261 tgtgcgtgat gcatctggtg ctgataacag cgcaaataca gtggacagat tttggcaggt
     4321 tgacaaaaca ggaaatggaa ccgcagccta tacttttact tatgcagcaa cagaaaatgc
     4381 agcaggtgga aatataaata ttagagcaca atcatggaat acaactaact taggatggga
     4441 tgctgcattg ccaggacagt taaaccctac ggtgcaaagt gttttagtac cgggggtaaa
     4501 tactaacggt gcctgggcac tggctttggc atcaaatcct ttaccaatag agttgctttc
     4561 gtttactgca aaatccaaag acaataaaaa agttgtttgc aactggagca cagctagcga
     4621 aatcaacaat gattttttta cggtgtttcg cagcaaagac ggaataaatt ttgaagaagt
     4681 tagcaaggtg gatggtagcg gaaccacaaa cactcaacac aattattctt ttactgacaa
     4741 caatccatat aaaggtgtat catactatcg cttaaagcaa accgatttta atggggacta
     4801 tactttttca cagattgaaa atgttttaat cactgatggc gatataaagt acttagtgta
     4861 tccaaatcct aacaacggaa atttctctgt taaatttgat gctgaacaaa acgtcaatgc
     4921 ggtattttta attacggatg caacaggacg gttaattcag agcggaagat tagatgattt
     4981 atcgggcaaa cagcaaatta atattgaaaa ggttttgcct ggattttatt ttttgcagat
     5041 aacaaatgga gataactcct acctcgcaaa agtgcaggtg ataaaataat cagccgttaa
     5101 ataaattttt tacttcagca attttcctgc gtgagatttc cacacgggtt tcatcctgaa
     5161 gaataggaag atggtcactg ttcagggctt ttaatttcag gttgcgcagc ccctgacagt
     5221 gtgatttaaa tttcaacact caaaacattc aaaactttcc aaaccttcga aacatacttt
     5281 gaaataataa aaaaacattg aagggttaat acgtttttga atccgtttca tagtcaccgc
     5341 gaaggggcgg gagactatga aactacaaaa acactgccct tgacagtgtg atttaaattt
     5401 caaccttcta aactttacaa ctttccaaac cttcgaaaca tactttgaaa taataaaaaa
     5461 acattgaatg gttaatatgc tttgaatccg tttcatagtc acaacgaagg ggcgggagac
     5521 tatgaaacta caaaagtaaa gattatggaa gaattattaa cgaaaaattc ctactattgt
     5581 aaacccaatt caggactaaa aataaattgt aaacttatat caggattaat taattaaact
     5641 gtaaactttt ttcaggacga gtcagtgctg catttcgctc aaatcatccc acaaccaaaa
     5701 aatgcaccac acaatttttc tcctccgcac cctatccaca tgcccaatct cattaattga
     5761 aattattccc ttatcaccgc taccttccta ctcacccttt tcccatcact cgttatcaca
     5821 caattataaa ccacattgca aaatgtaagc atctttattc ttttactaac tttaatacga
     5881 tagatatttt gctttcccaa acaacgctgc aaaagtatat cccagaagca agcgatgaaa
     5941 tatttaattg ttttgcattt tcgagaggag aagttgaaaa agtacgaaca agatttccgc
     6001 ttgaatcatg taatttaatt attcctgaaa ctttttgcaa cggaaactga atagaataaa
     6061 ttccattgga aatagtagga agtatctgga tcggcactaa agaaatttga ttctcaattc
     6121 cagtgcatgc tgaaagtatt ctataacccg ttactataca gccaccactt ccggtggccg
     6181 aaaaccaata actgcccgga gcgtttactg ttattgatga agtagtagct cctgtattcc
     6241 aaaggtaatt ggttaatccg ttaggtgcat ttagcataag agagcttcct gcacataata
     6301 cagagtcatt gcctatattt acaaaacctg gattccctgt aaatgtctta aacccctcaa
     6361 tatttggtgc tgaataaatg gtatcatgac ttgcccaaat agaggtcata taaacatttc
     6421 cttgtagggc ttgcgtaaac caacaagtgg cgatgccggt tgaaatatca atctgggcta
     6481 tgcagtatct gtttaatcct cctaatacaa cggaattacc ccccacccat aaagtgtttc
     6541 cctgcaaggt aagtgcatca attaaggagt tgctgcaata tatatttttg ctggtaacaa
     6601 aaccagatgt atcaatttca gctatccctt ttcttgtcaa accatcaata gcgctgaaat
     6661 tacctgatac atataatttt ccattctgag ccgctatttg attcaaataa tcggaattgc
     6721 taaagctaaa ggaaaaagta gtatcaatga ttccggtaag catatcaaac cggcacaggt
     6781 ttttaattgt agaattagtt aaataatggt taaaccatcc tgctgcataa atataatttc
     6841 ctgaagatat taattcacat ttatttacat taaaattgct gctaacccat atagtatcca
     6901 ataatagcgt atttaaatca aatcgtctga ccgcatcaac acttccaaaa atatcgcttg
     6961 cagtaaaata aacaaaattt cctgcattat ataaaccgta tcttccaatt ccaattaaat
     7021 ttaacgggat agagttaagg tttagtaagt ttccgctttg taaatcataa acagctatac
     7081 gatcaatcaa actccctgag atgcttgaaa aatctcctaa aacaaccaat tttgaattac
     7141 aaattatcat tttcgtaact tgtgcattgg gtacaggatt ccatggcaat atagcgcctg
     7201 tatttgcatc aattgcagca aggtatggac gggcactcgt tcctactgta tcaaattctc
     7261 ctccaatata aatcgtattg ttatatcgta caatacaatt tacacggtcg ttagctgtgg
     7321 gaatattggg ctgaacagtt tgtgagttca cttttacaga aagaataagt aatgcaaaaa
     7381 cggttacata atttttcatt gaatttgttt ttttattgtt attgtacaat atcgtcctaa
     7441 ataaaaaaga tagagtagta ataaagtaac tcaaagttat ctttgaggtt gaacaacaat
     7501 aatacttctc tgatgacaaa tctaagttta ttttccctac tttcaaagtc tccctcattt
     7561 gagaggactt tgaaataata aaaaaaacat tgaagggtaa atacgttttg aatccgtttc
     7621 atagtcaccg cgaaggggcg ggagactatg aaactataat gtatttgata gttctattgt
     7681 tttgcaataa aattaacctc tcataaaaat aattttacaa ccaccttctt actttctttt
     7741 tataattttc atattcctgt tcgaattcaa ttgccaaata tgattcttcg cgtttgataa
     7801 tgtattcctg aacaattaaa attagtatag gaaacagaat aatattccac cagttgccaa
     7861 tgaaaatagt cacgcctaaa taaacaattg ctaatcctac atacatcgga tttcttgtaa
     7921 tggcaaaaat gcctgttgtt tgtaatgaag atgccggttt aatcagaatt atggtgtttt
     7981 tggtaataaa aaactgcctt aaacttctga acaggaaaaa cagcgctgtg ataaaaagga
     8041 ttataccgag tgattttatt actaatgtat gcaatgccga atcaagaata ggaactttct
     8101 tctgtatata tacggcagct acaaaaatta aaacatagaa taatggcggt ggtatgtaaa
     8161 cacccggacc ttttgatgta tcaacctgtt tgttcattgt gctgttgtgt ttttcaatgt
     8221 taaatcatta tatcattctt gttttttaat attaacataa ttgattttta gttgtttcat
     8281 taaatcttcc agatagtcaa tctgcacttc ctgtatttct aataattttt tgttttgatg
     8341 tactaataag tgatctattt tttcacttag cagttttatt tcaagttctg ctttaagatt
     8401 aattttatag tcatgctctc ctctttgtct gtctttttgt tcctgcctgt tttggctcat
     8461 cattattata ggtgcttgta tggctgccag acaagaaagt attaagttta ataggataaa
     8521 tgggtaaggg tcaaatggct tacttttaag taaccaaatg tttatgatta tccacaaaag
     8581 cagaaaaaag aaaaacgaaa gaataaatgt ccagcttcca ccaaaagaag caattttatc
     8641 agcaagtttt tgacccattg tgagtttggc ttcaatttca tcctgaatat tttcagaaag
     8701 aatagaattg tcgcgaatgg cttgcattac atctaaatca atcgtagcta tttcgccttt
     8761 ttcctgttca acaagtttgg tcagataaag tcttctgtac ttattcaatt catcaattga
     8821 aataatacta tttgcatcga agtttgggaa gtcacttttt atcaaaagaa aaattccttc
     8881 tctgatttcc tgacctttta caatttcacc ttttgaaatt actttatttg atatataact
     8941 taacggcatt ttatattcat taaatgaaat tattcttttt tcttaatgta aaagtatttg
     9001 ttttaagaca tcaaactttc aatacaatta tattttaatg aatacatcaa tcaatgataa
     9061 agaatttttc agcacttcca tcactgaata tttttattaa aggggtgttt gtttttaatg
     9121 gtgattcacg tcccaataaa tctataattt ttataacctt tcttttaatg tcagttgttg
     9181 tctcagctat acctgttgtc aggcaggctg gggaataacc cgatgatgca tttaatgaaa
     9241 gtgcatagtt gtaaagttga tcacagatgt ttagtccgtt gccttcgcca ttgcttaaaa
     9301 cacaaagccc tattttgttt acagggtcga ggtatatttc agttgaaaca ccactttcgc
     9361 caccattatg tccccacacc caggcagaac caccattata atacaacact tctttatacc
     9421 agtttaaacc ctgacgtgtg ttgagtgatg gaaactgtac tgtccacatc tgattgatgg
     9481 atgcaggcga taaaattgaa tttgtaccaa atgttccacc atttaaatag gcaatcataa
     9541 aattggctaa gtcagtaaca ttgcttcgca gttgcccgtc aggataatcg gcaaaaccat
     9601 actgcggata ggcaacataa ttatttcctg aataatgata tgggatggca acatcgtttg
     9661 gattaaaatt agaataatac catcttgttt tattcatgca aagtttgtta aagatgttta
     9721 tgttgcaata ttgatcaaac ggtattccac ttgcgcgttc aaccaaatat ccgtttaatg
     9781 ctgttgctat gtttgagtat tgaaacacac ttccgggagc agttgcaaga aaatttgctg
     9841 atgcattata atcgcttccg ctggtagaaa aatagcgctg catacaatca tctaaagtta
     9901 ttgtagggtc gggataacca tagtagccaa ccatagccgc ccagttatcc tgtatggatg
     9961 aagtatgtgt catcagctgg cggaatgaaa tagtgtcgtt gggataaccg ggtatttgta
    10021 acagccaagg taaatattga ttgacatctc catcaagatt tatcacattg ttttcgtaca
    10081 actgcatggc agcagtgcct gtaaaaactt ttgatacaga tgccattaaa aaaacagttg
    10141 tgtcgctaac ggggatagta tttgcaacat cggcaaatcc ataagagtta atccaaacaa
    10201 ttttatcatc cttaacaatc acggttgaaa cacccgggaa atgttgtgta ttcatttcgc
    10261 tctgaataaa agcatcaaca gaagtgcttg gattgggatt agactgacta aaagcaggta
    10321 aaacaacggt taagaaaaca atgatgaatc cttgtatctt aactattcgc ttagtcactg
    10381 tttttatatt ttaaaagctt acataagctt tgtgctttgc gaatatacga ttgttactgt
    10441 aaactattgt tactttattc ttgagtaaca attaatttct tacggtaact tgcttcacca
    10501 tttaatacgt ttacaaagta aactccggct gatatgtttc tcaggtttat tagtttttgt
    10561 gattcgttat tgataacttc acaaccaaca atttctccaa ctgcattata aatgttaacc
    10621 ttaccggtag gaattagttt atcaaacgaa atagtaaact gaccgtttgt agggttgggt
    10681 gaaagctgat atatgagatt gctattttct tttatactga cagtggtatc accacaaata
    10741 aaaacatcag cagcaattga tggtgttgaa accattatct gatttaatat aggaaaaggt
    10801 gaaccgattg ttggactaaa tatatttaca attcccggcc aatttgcact tgatggtgta
    10861 tttgtgaatt gcgttgttga acagcctgaa ttaccaatgc tatccgtctt tacaatataa
    10921 atatccttac caccaacacc aaaacttgtt gcataaccgg caataataaa tccgttatca
    10981 gttgtgtttt taacagcaaa ggagttgtct tcatctgctc ctccgtagtt tttgctccag
    11041 agaatatttc ctgttgaatc aatttttacg atagtcattt tgctggcgct gcttggtgca
    11101 acatcacata ttacggctgc aaaaccatta tcatgtgtta aacaacctga ttgagaaata
    11161 aagcgtcttc cggattcacc aatgtttttc gaccaaatga gattaccatc actgtcatat
    11221 tttataatct tagcatcaga ttgtccgata gcattattcc aggtgttgcc tgtagttatg
    11281 aaaccatcgt ttggtaaaat attcagtgag tagctgccat cattgttaat ttctccaata
    11341 gccttcgacc agattaatgt tccggacgag cttgttttca taatgtaatc atcaatcagg
    11401 cccgcaccaa agccacctac tgatccaatt aaaattaaat cgccattaga aagttcagca
    11461 acatcactgc cataaagata tcctccaaaa cttgactgcc aaagcatagt ccctgctaca
    11521 ctaaactttg caaggacagt attgcttcct gcagttccgc caactgatgc aagaagaagc
    11581 gtactatcga tagcctgctt taaatggatt ctccagtttt tattcggtac tgtataattt
    11641 atattaaaac tgttgaacca ctgaatatca cctgatgcat tcagcttaca gcaaaaaata
    11701 ttttcattgc cgtttccatt atcgtcataa ataccgcaag cataatattc accactaaat
    11761 gtttgtttaa tatccataat ataatcatcg ttggtgctgc caaatgattt tgtccattgc
    11821 atatttcctg ttgcatcaat tttaagaacc aaagcatcct tgcctccgtt tcccaaattg
    11881 gaagtatagc cgccaataat gtagccgcca tcacttgtta aattaacgga ataggcttct
    11941 tcgtcactga cactcccata aactttttga aatttaacct gagcttctgc cagagaaaca
    12001 aagcaaaaat tagcaattac gagtaatagt tttttcatat attttatttg gtattaattg
    12061 tttttttcgg aaagataaat taaaagtgtt acttaaaaat ctgcagcaaa atactgtgaa
    12121 agaaataata aaccaatagt taaaattatt tttaattagt tgatttgtat tgcactcaca
    12181 atgaatgaga ttgtgttata agaatgtagc accttaaaat ttaaatacct tcgcatcttt
    12241 caaaataaat tttatattac aagtatgggt ttcaaaactt tggataacat agctcaattg
    12301 aatgaaattg acacattatc aaaaaataag aagcaaatta tatttaagca tagcacccga
    12361 tgttcggtaa gtacttttgc taaaaaagtt cttttgtcag aattttctga tgatatggag
    12421 aaaaaatttg atgtgtatta tctcgattta ctcaattaca gagaggtgag taataaaatt
    12481 gcaaatcact atggtgtgta tcatgagtca ccgcaaattt taattatttc tgacgggaaa
    12541 tgtatttatg atgcttcaca tgatgatgtt tcattggcat cagcattggc atcggtcaat
    12601 taggactaat ttattgacag cgaaaaccca gcagcaacac atcgtcaacc tgatcgtttt
    12661 tgccgcgcca cttttttaaa tagtcagtaa agaaagttaa ttgctctttc atgggtaagt
    12721 tatgtttttc aagaagtagt tctttgagtt tgcctgacat gagcttttta tttttctcgc
    12781 ctccaaactg atcggcatag ccatcagtaa aaagatatac agtgcctgca tcgtcaaatg
    12841 aaatggtttg attgataaat tcctgttgtt gcaaaatgtt gttgccgcca ataactattt
    12901 tattccctga aagtgtttta agttcattgc ctgaaatgaa cacaagcgga cggtaagctc
    12961 ctgcaaatgt aatttgcttg gcactgcaat tgatatgaca tacggcaatg tccattcctt
    13021 cggcagcatg agtttctgcc tgattaagcg aacgcgaaac ttcaattcgc aactgattta
    13081 acaattctgc aggacgggta atatttttat ctctgacaag atgattgagc agcgatattc
    13141 ccaacatact cagcaatgca cctgcaacac cgtgaccggt acaatctgct actataacga
    13201 tgtgcccatc ttcaacaggt tcaatccaat aaaaatcacc agcaacaata tctttaggct
    13261 gatacagtac aaaactttct ttaaagaagg atgctaatgt ttcttcagag ggaaggatag
    13321 cgcgttgcaa gtaacttgca tagtgaatgg agtcaagaat ttctttgttt ttttcttcta
    13381 ccaatagttt ttgctgttgc agttcggcag ttcgttgttg aacctgagtt tcgagaagtt
    13441 cgttttgctg aagtattaat ttttcttttt ctaaaagtgc atccaatgct ttttgttgtg
    13501 cttcggcttt ctgaagcaat gcatcatcaa ggttgcgcga tgcttcaatt ccttttcgaa
    13561 gcatttccat ctctaaaaca gtttttttga tggttgcttc aaggtcggca aggtcaattg
    13621 gtttggtaat aaaatcaaaa gcaccacgat tcattgcagt acggatattg ctcaaatcgg
    13681 cataagcaga aacaacaaca gctttagtaa gaatattatt ctctctgagt ttttcaagta
    13741 aagtcaaccc atccatcaca ggcatgttga tgtctgtaag catcaggtca tagctgtcat
    13801 cattctttaa tttttctaat gcaattttac cattagacac atattcaaat tcaaattgat
    13861 tttctttaat ctgctttctg aatttttgtc tgactaaatc ttctatgtaa ggctcgtcat
    13921 ctactaataa tattttgctt ggcataagtt agggtatttt tattccaatt actaaaatgt
    13981 catcaacctg atcatgtttt ccttgccatt taattaatgt attcaataaa tattctttct
    14041 gttcattcat actcagatgt tgaatggata ataacagttc acgaaacttt tttgtcataa
    14101 actttttacc aaattctcca ccaaactgat caacaaagcc atcagaaaaa aggtagatag
    14161 catcaccttg ctgaagtgtg atgttatgtt gcataaaacc gtcagctttg ttgtactgct
    14221 gtccgccaat gggtattttg tttggtttat aaaactgaaa ttcgtttttt cgaatgagcc
    14281 aaagcggtcg gttggcaccg gaatattgta aggttttgtt tttgttgttt attgtacaaa
    14341 gcgcaatatc cataccttca ttcatctcgc tttcgcgttg tttgagtgat gttacaatgc
    14401 catcattaag ttgtgtaagt attttagatg gctctgtaat gcctttttca tttacaatct
    14461 gattgagcaa tgcagttcca atcatactca taaatgcacc tgcaactcca tgtcctgtgc
    14521 aatctgctgc cgctacaata attttttcat ctcgttgagc aaaactgtaa aaatcaccac
    14581 ttacaatatc ttttggaaga taaagtataa atgaatcatt aagtgattgg tttattttct
    14641 ttatgtcggg caatattgca gcctgaatgc gtttggcata gtggaggctt tctgttactt
    14701 cacgattttt tatctcaatt acattttttt gttctaatac ttcatgagta cgttccttta
    14761 cttccgattc aagcattgct ttctgatttt ccagcaaatg ctttttttct tgttcttgcc
    14821 tgatggttct ggtcgaaagt tcagttattt catctatctg ctttgttaat gtattgccca
    14881 tgcgtgcaaa gtcgcgtgca agatataatg ctacagaaaa tggcaaaccc attaacatac
    14941 ttacagaaat gaagttagaa gttttgactt caaatttcat cccccaaaaa gttattgtgt
    15001 caggtaaaat actgataatt attcctaaga ataccggtag gagtaaggtt agtgcaaaaa
    15061 tccaggcgcc atctcttttc tttcttacag atacaatgat tactctgaat atttcaacaa
    15121 atgacgttat aataataaca gccattagta tagcggctat tttgcgaaat ggcaataagt
    15181 aaagtacaat cattactgca taaagcccaa gcagtaccca taaatttctt cgaaaatgat
    15241 gataaaatat ggtgtgtaac atcgctacaa tgggcaccac tattaacggt gctaaaaact
    15301 gaagtgactt agagataaaa gcaattgatg aataatttga tgaagttaat aaatgatagg
    15361 tgtagaaagt aaaaataaag atgccaatgc aatagagact atagtacagg ttagtgattt
    15421 tttgcctata gtaaagaaac ataatgaggt gaactattgc taatgtgaga aaaactgctg
    15481 cgacaaaaag aattaacgat gattttttta tagtttgaat gctgtcgtca atttcactat
    15541 taaggtcttt gaatttgatt gtaaagtttt caccaatatt aaatccgctt attctggcag
    15601 aggaattgtg aaaatttgaa taacgtatgg caagaacgtg attatttaaa tttgaaaata
    15661 ctacagcaac gggttgtgga ttggtattga aaacagcttc ctcttcagaa atattagaag
    15721 caacagtgcc atattttttt aatagtctgc catctaaata tacctctgct gccgaaccat
    15781 ataaatgtaa aattaatgca agaggacggt tcaataaact actgtcaata tcaaaatgca
    15841 tacggtacca aactatcgta gtataatttt taatgtgatt acttctgata gtatctgaat
    15901 attcctccca tacagaatcg tcatatatgg gttctttcca taaggtatca tccccacgtt
    15961 taaatttcca gttattggta aactccaaca gattttcatt tctttcaaat acctgaatgg
    16021 tagaatctgc atttgcctga gcatcctcat catccgatag caaattgcca agattgacct
    16081 tgatatttac atttgagtcg agatcagatt ttttattgga attctgacca atgacattaa
    16141 aacaaattcc tgaaaagaaa atagaaagaa ataaccggtg caggttaaac atctgtaaat
    16201 tgaatttgca gtaaaggtaa ataaataact acttacagaa tgttgaccaa actgttacct
    16261 gactatttta acaaatcaat aatctttttt tctacaacgc tgctattcca gatgttttca
    16321 atgtcggcca ttttcataat ttcattcata atagcatctt gtctttttcc tcttgcctgt
    16381 gcctcatgat aaggaatgtg gctgaatgta accatggtat aaagtggaat ccatttgtca
    16441 ggatgttttg tgctgaacca tccttcaatt tttttgcgta ataagaattc aggcttgccc
    16501 actaaatcgc gcatctcaat aaaattgtca acggcaagtt cgccaatggc atcggcagca
    16561 ggttttctca ttcgttcgta ttcgggtatt actttctgcc agtcgtcagc atattgattg
    16621 agtaggtcgt tcaacacact gcaatcttca aaaccacagt tcattccctg accatagaat
    16681 ggcacaatgg catgtgctgc atcaccgata aggacaactt tgttatcaaa actccatggt
    16741 gcacacttaa cagtcaccaa tgaacctgtt gaattgtgaa aaaaatcttc ttcaagatcg
    16801 tgtagtaatg gaacagcatc aggaaaaact ttttgaaaga aagtttttac ttcttgtttg
    16861 ttgtgaagcg attcaaaaga aggattccct tttaatggaa agaatagtgt aacggtaaat
    16921 gttccatcaa gattaggtag tgctatgagc atatatccgc cacgaggcca gatgtgaagg
    16981 gcgttttttt cgagttgatg actgccatcg gctttagcaa caaacatcag ctctttatag
    17041 ccgtgttgca gataactctg cgaatagttg aactgttctg ttgatagctg tagctgaagt
    17101 cgtgaagcag caaaagcacc atcggcacca aaaatcaagt cggaggcaaa ggttgttttc
    17161 tgatgactct ctgagttttc gaaatgcatt tcgcctgatg caagtgttaa gcccgtacat
    17221 ctctgattaa aatgaatttc aacaccacct ttttctgcaa gatcaagtag ggtacagttt
    17281 aaaattcctc ttgaaactgc gtaaatgccc tgaccgtctt ttccgtaggg ttgtgtggtt
    17341 atttttccat caacactatg catggtgcgg tgtgtcatcg gtattccgat tttacggatt
    17401 gcatcatcaa taccaactcc ctgcaaacct cgccagccgc ggtcgctcaa tgcaaggtta
    17461 atacttcttc ctgcagggat ttttactttg cgcatatcag gtctgcgctc gtgaagtgta
    17521 actttatatc ctcgtttatt tagatataat gccagcaaag aaccaacaag accggcacct
    17581 atgattgaaa tttttttcat tgttgttatc tgtttagaag caaagcttcg tgcaggcatt
    17641 tgccaaattg ataaatgtcg ttaaatgaat tgtaaagcgg aacaggagca atgcgtaaca
    17701 cttcaggctc gcgccagtct gcaataatgc cttgctctgt caggtagcca tgaatttttt
    17761 tgccgttatg tttacatagc atactcacct gtgcagcacg ttcgttttca ttttcaggtg
    17821 ttatcatgat gatatttttt tctgaatttg tcttgctgat aatgtcacca attataaatc
    17881 gcagaaagtc attcatcagc ttgcgttttt ttatcagatt ttgtactccg gcttcggcaa
    17941 aaatttcaag tgaggcacgc aatgctgcca ttggaagcac aggggcatta ctcaactgcc
    18001 aagaatcggc acttttaaca ggaataaaat catgcggcat agtgaaacgt gtttgtggat
    18061 cgttgcccca ccaacctgca aatcgaatca gatcggtgtt gttacaatgt tgttgatgaa
    18121 cataagcacc tgcaacacca ccgggtccgg cattgaggta cttgtaacta caccatactg
    18181 caaagtcaac attccagtcg tgaagatgca attcaagatt acctatgcca tgagctaaat
    18241 cgaaacctac aattgctcct gcagcatgtc ctgcctgagt aattcctgcc atatcaaaaa
    18301 cctgccccgt atagtaattt gggttaccta aaaaaaccaa gaccagctca tctttatgct
    18361 ttgaaattgt ttcaagaata tcttcatgcc tgatggtatg ttcacctgtg cgcggatgca
    18421 actcaatgat ggcattatca ggattaaagc cgtgaaattt tacttgtgtt tgtaatgcat
    18481 actgatcaga tggaaatgcc gacccttcac agattatttt aaaacgtttt gttgttggtt
    18541 tgtaaaacga caccaacatg aaatgaatat tggcagtgag tgaattcata accacggttt
    18601 ctgacggtag tgcacctgct ataagtgcca tattatctct cagaaattcg tgatatggat
    18661 accatgggtt ttttgcctga aaatgtcctt caacaccgaa atctttccag tcgttcaact
    18721 cctgcaaaac atagtcgcgc gtttgtttag gttgaagtcc aagagagttg ccgcagaaat
    18781 agatgacatc tttattctga tgttgcggaa aaagaaattt ctgcctgaag gattttaaag
    18841 ggtcttgctc atcaagttga ttggcaaaat cggatgaatt gataaagtgc atgtaagtaa
    18901 tttgaataca aataaacaaa aaacattatc gaaaggatat aaaaacctgc tggtaaaaag
    18961 taaaattcaa ttttgtaaaa aagcgctgac taatgcctcg gcttctttgc atgattgatt
    19021 caattctcca ttgataataa cattatcgaa acggtcttgg tatgacaatt ctttaatgga
    19081 tttattaagg cgaatttcaa gagattctgc tgtttctgta ttgcgtgccg ttagtctttg
    19141 acgaaggtct tcaaccgatg gcggccgtat gaaaacagca agtaaatttt tgccaaaatg
    19201 ttttttaatc tttaatcctc cttcaacatc aacatcaaaa actactgaac agttaagcgc
    19261 ttctatacgt tcaacctctg attttaatgt gccatagtaa cgaccttcat acacttgctc
    19321 ccattcaaca aagtcattcc tgtcaatatg ttttctgaaa ttttctatag acataaaata
    19381 ataatctttt ccgtcagttt cattagcacg ctttgggcgt gtagttgctg aaatactgaa
    19441 ttgcataaaa tcaaacttcg ttaacagatg ttgtacgatg cttgttttgc cactaccact
    19501 cgggccacag aaaagaaaat gttttgtcat aaaatattag agagctgttc tttaattttt
    19561 tcagcttcgt ctttcatctc aacaactttg cgttgtatgt ctgcatcatt agctttgctg
    19621 ccaatagtat ttatttcacg accaatttcc tgcataataa agcctaactt tttgccatta
    19681 ttattttcat cattcaacat agataaaaaa tagcggcaat gctctgcaag tctcactttt
    19741 tcttctgtaa tgtctaattt ttcgagataa taaatcaact cttgttcaaa acggtttttg
    19801 tctgtttcta tatttccgcc aagagacttt aatgagctga ttagtttact gcgcactgca
    19861 tttattcgcc cttcctcaaa aggagttatt tgttcaagta attgcaaaat aagattcatg
    19921 cgttgcgaaa tatcttgttg cagattatgt ccttcgcgct gacggaaaag attaaatttt
    19981 tctattgcct gatttattat tgaaaaaata tatttccatt cattttcatc agcgtcatca
    20041 gaatcggtat ttactacctg aggcatttgc attacaattt gcaaaaggtt gtcatcgggc
    20101 aatgcaaatt cttttttaac tgcttttaaa tcggtcaaca gtgattttat tatttcacgg
    20161 ttgagtaaat tttcttgttg cgcaagatcg gactccatag taatcatcac atccatcttt
    20221 cctctttcag cttcttttaa taacaagctg cgcaggtcca tttctttatc tttgtaagga
    20281 tgtggcaggc gcatgttaag atcaaaaaat ttactgttga gcgattttat ctcaacagag
    20341 atagtcttac tcttatagtc accggaggct ttgccatagc cggtcatgga acgaatcata
    20401 cttcacaaaa ttttggctaa agtaatcaga atgataacag caacatcagc tattctcagt
    20461 attatttata cctgcaattt tttgcttact tactaaatag acacctgtaa aaattaaaac
    20521 agcagaggct aagtgcagcc aacccggact ggcttcgcca agagccattg aaattagtgt
    20581 agcaaaaaca ggctgaaaat aaatgtagat actcactaca gaagggctga gatgacgcaa
    20641 tgcaaacaca ttcatcagat aagcaacaaa agttacggct acaataacaa agagtatgca
    20701 aagccataag ttaaaagtca ggctgctcca tgctaccgct gttacttcat taaatccaaa
    20761 aggcaaaacc attaaggcac caaaaaagaa tgtccatttc atgacagtta taggatggta
    20821 gtgcaacatg agtggtttta ccaaaataat aaatatggca aatgaaacag agtttaagaa
    20881 aatataaaga tcaccttctg tgtttcctcc ctgaagattg aagttcttac ccaaaagtat
    20941 gagtaacata gcaccggcaa taccaagggt aattccaata tactttatcc atcccacagg
    21001 ttctttaagt attatggcag ccatcagtaa aaccaaaaca gggtttgttg tcattattaa
    21061 tgcaccgttt atgggtgatg tgagtgaaag acctttaaaa aacaaaagtt gattgatggc
    21121 aacgccaaag agtgcacaga gaagaatagt gtttctgtct ttttttttga ttttttctga
    21181 tggtattaaa aatgctgcaa gaaaaaacaa aactgtagcc ggaattactc tgatgacaat
    21241 aaatccaaaa ggtttaataa attcaggcat cacctgtttg gcaatgctga agtttgccgc
    21301 ataaagcagg ttaacaaaaa gaacagaaag gtgagcgcgt gttgtggctt tcaagcctta
    21361 gtgatttttt gtttgatttt tatttttatg cattgcagct ttagctgcag caactgtttt
    21421 tggtgcgttt ccaataaaaa tttctttgtc tatgatgata accggtcgtt tcagaaaagt
    21481 atattcttca agaatgtatt ttttgtagtc tttctctgaa agttttatct gattgagtcc
    21541 cattgaacgg tatttcatgg ctactctgct aaataacgcc tcatagctgc ctgcaagttt
    21601 ttgcattgca tcaagctgct gttccgtgat gggttcactt tttatatcct gtaaaataaa
    21661 atcttttccg ggtttcagct ctgttaatat tctctgacag gtggtgcagt tggcaagatg
    21721 atatatcttt ttcattcaac tttcttgtgt aaaatagttt tacaaagaac atgaaaaaac
    21781 atcagatttt ggtattcctt atcttatgtt gtccatcacc ggcaatttgt tttatcatat
    21841 tgcggaaaat aaaaaaatga aaaggcaaca tggaatacca gtaattcctg cccataatac
    21901 caatgggtct gaaagtagca gtttgtttca gaatacaacg gttgttgtta tgaatgatag
    21961 agaactctaa ccatgcttcg cccggaagtt tcatttctgc atacaataac aatcggtgat
    22021 tttttctgtc aaccaacagt acacgccaga agtcaagagt gtcacccggt tcaatgtcaa
    22081 catcacttct tcgtccgcga ctcatgccaa cgccacctat tagtttgtca attattccac
    22141 gcattctcca taatgtatcg gcatagtacc atccattttg tccaccaata ttaaaaaagc
    22201 ggttggctac ttcatcagct ttgtctttgt ctatttcaac ccacttgcgg tctttataac
    22261 atccgtttga cggtacttca atatgttggt taacatctaa gcgactcaaa ctgctcgatg
    22321 ccgaatcggt ccaacttgaa ataaccatgt tttgttctat ccgtgcaaac gccatttgta
    22381 ttgcttcgcg ataggtgtat aactcctgag gaataacatc acgaacagaa gtttctttac
    22441 acaccacatc atttttcatg ctctgaacta aaagctttgc aatggtgtag ttggcaggtg
    22501 tagaaaaata aatccatgtt gcagataagc ccatgaaacc aaaaggcagt gtaatgatta
    22561 atcttttata tcctctttct gctgcatatt caagcaacat tttacggtaa gtgagtacat
    22621 cgggtcctcc aatttcaaaa actctgttaa aagctttttc attcatcagc gaaaggtgca
    22681 ggtagttcat cacatttctg atggctatgg gttggcattt tgatttcagc caacgtggaa
    22741 caatcataac aggaattttt tctgttaatc ctctgatgat ttcaaaagaa atactaccgg
    22801 agcctacaat aatacctgct tcaaatatag tgtaggcaat gcctgaattt ataaaaacat
    22861 ctttcacccc cttgcgtgct gtcaggtgtt tggaaaggtt atcttcattg ctgatgccac
    22921 tcagataaat gatttgttta acctgcgtga gcgtggctac caatacaaaa catcctgcac
    22981 agcgcagttc atattcttta agcgttgtgg ccgtatcgcc taatgagtga attaaataat
    23041 acgcaacatc aaattgcttg ttgttaaaat gctgaatggc gttttcaggt tccagaaaat
    23101 catattcaat aacatgtatt ttatcaagta aatcaggcga tggtttaaag cgaagtgcat
    23161 ctctcaccac acaggtaaca tcatgtcctg cttctaccaa aacaggcagc agcctcatgc
    23221 caatatatcc gtttgcccca gtaagtaata tacgcatctt atacttgatg ttttacgatt
    23281 cttcaatcct tgcaaacata gcaggatatt tgatttcatg aaatgatatt gattagtaat
    23341 ttttaaattt aaggaaatca ttttcagaaa tgggattgaa tagaaatgaa ttttctatct
    23401 ttgaatctta aattttcgta aatggcaaag cgaacagcaa agcaaaatac caggttgaaa
    23461 tttgatacgg catggcagta ttcagcagca cctgaaactc cggatcacat tgtgttagaa
    23521 gacaaataca atctatttat caatggtaaa tttgttgcac cacagagcaa aaaatatttt
    23581 gacaccatta accctgcaaa agagacgcga atagcatctg ttgcagaagc atcgcaagcc
    23641 gatgtagatg cagcagtaaa ggcagctcgt acagcttatg ataaagtgtg gagcaaaatg
    23701 ccggcacgcg agagagggaa atatatttac cgtattgccc gcattatgca ggagcgtgcc
    23761 cgcgaacttg ccattattga aagtttagat ggtggaaagc ctattcgcga aagcagagat
    23821 attgatgttc ctttagcagc agcacacttt ttttattatg caggatgggc tgataaactt
    23881 gattacgcat tacccggaat ggcagcacaa cctttaggtg ttgccggaca aattatccca
    23941 tggaattttc cgatattaat ggcagcatgg aaaattgcac ctgcacttgc ctgtggaaat
    24001 actgttgtgc taaaacctgc agaaacaact ccacttacag cattaaaatt ggcagaaatt
    24061 ttcagagatg ccgaccttcc tcccggtgta gtgaacattg ttacaggagc aggagccact
    24121 ggagcagcta ttgttaatca tcccggtatt gataaagtag cttttaccgg ttctactgat
    24181 gttggaaaaa ttattcagaa agcattagca ggaacaacaa aaaaatacac ccttgaactt
    24241 ggaggcaagg cagctaacat catttttgaa gatgctgcca ttgatcaggc tgttgaaggc
    24301 attgttaacg gaatattttt taatcaggga catgtttgtt gtgcaggctc acgattgttt
    24361 gttcaggaaa gtgttgccaa cgaagttgtg cgcaaactca aagaccgtat ggaaacactc
    24421 atcattggcg atccattaga ta
//
LOCUS       DAOOOL010000024        24142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_162_length_24142_cov_114.828704, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000024 DAOOOL010000000
VERSION     DAOOOL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24142
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_24142_cov_114.828704"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="dapF"
                     /locus_tag="PKU82_03505"
     CDS             <1..547
                     /gene="dapF"
                     /locus_tag="PKU82_03505"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOZ81971.1"
                     /translation="FSKQLGMIGRETLFMAYDGLHSAKVSAAKALKSAIVSVSMSDVS
                     EIKKHNGNFILNTGSPHYVKFVDDTDKIDVVKEGKKIRNSNTFKAKGINVNFVSFKSK
                     ASNIRTYERGVEDETLACGTGITASAIAAHYSGLTKKLSVNVIARGGSLNVTFQVEAN
                     KYKNVMLTGSAMNVFDGEIKI"
     gene            complement(537..2993)
                     /locus_tag="PKU82_03510"
     CDS             complement(537..2993)
                     /locus_tag="PKU82_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ81972.1"
                     /translation="MMIKKFSLLLLALCLSLYSNAQLDLSGVVSTNDGKKVDHAVVTL
                     KESLIRHFSDEKGKFTFFNLKPGKYTLQVNQLGYHEYSSEINLASNQHIEVILFKKAF
                     VTDEVIIQSLKMKNKIPIAQNSLNKNEIEEMNSGKDVPEIMDQLTSVVVSSDAGAGVG
                     YTGMRVRGSDATRINVTINGIPINDAESQSMYWVDMPDLLSSTNQIQLQRGVGTSVNG
                     AAAFGASINILTDNLSEKPFAETNLSYGAFNTKKVNLIAGTGLLNNRFAFETRLSSIQ
                     SDGYVDRASSNLKSFYFSGGYFGKKSMLKAIIFSGIETTYQSWYGVPESRYKNNMQGM
                     MDYISRNGLDSEDATNLLESGRKYNYYLYPGQTDNYQQDNYQLHFSHQINNLFTLSVA
                     AHLTRGKGYYEELKKNAKFSDYGLPPIQIQDTIITKTNLVRRKWLDNYFYGLIYSLTY
                     ENKNLEIKTGGGINQYDGDHYGKIIKGDIIPLASENYTYYQNNGLKNNFNHWIKGTLH
                     LGEHFIAYADIQYRYVHYRFTGFDDFATTTNQVAEFHFFNPKAGITFLRNENESAWLS
                     IAVGNKEPSRDDFTESTAKSRPKSENLIDFEAGYSIAIKKLKLSLNAFYMQYKDQLIL
                     TGKINDVGNYTRQNVKNSFRRGLEAEFNYDVGKFLAVSGNVALSENKILNFKEYIDQY
                     DADFNYTGQQKIIYKKTDIAFSPSIITAVGIEIRPLKMLSIKLTEKYVGKQYLDNTSS
                     EEKSLKAFAYTNLSIHYTFKIFKTIETEAGVAVYNLFNEMYSSNGYTYGYLYDGMRVN
                     ENFYYPQAGTHTMLTAKFRF"
     regulatory      complement(3037..3137)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3296..4159
                     /locus_tag="PKU82_03515"
     CDS             3296..4159
                     /locus_tag="PKU82_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOZ81973.1"
                     /translation="MRQLRIQKSITNRESQSLEKYLQEISREGMITAEEEANLARRIR
                     EGDEEALSKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAASRFDETRGFKF
                     ISYAVWWIRQSILQAIAEQARIVRLPLNQVGSLRKINKVFSKLEQEFEREPSAEEIAE
                     MMEVPIEKISDTLKSPGKHVSMDAPFAGNEEATLLDVMQNDDVPDADFGLMNESLRKE
                     IDRSLKTLTERERDVVKLFYGIGVSHSFSLDEIGAKFGLTRERVRQIKEKAIRRLRSG
                     SRNKLLKDYLA"
     assembly_gap    4364..4463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4464..5111
                     /locus_tag="PKU82_03520"
     CDS             <4464..5111
                     /locus_tag="PKU82_03520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ81974.1"
                     /translation="YGNDVKVVRNVPDRTDSVNFDVAKWKRSVGFPDYKKVFIMQGAG
                     INVDRGAEEAIEAMKDVDAMLLIIGGGDVFHQLQELAIKHNVKHKVIIHPKISREELI
                     TVTASCFAGLTLDKDTNINYKFSLPNKLFDYIHAGIPVIASDLPEVTAIVNGYKTGIV
                     LPQYSPQAIAEAMNRLVIEKELYVSYKENCSIASKDLCWQKEKKILIDVILSSEK"
     gene            5135..6259
                     /locus_tag="PKU82_03525"
     CDS             5135..6259
                     /locus_tag="PKU82_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ81975.1"
                     /translation="MKNSGKHLHIISFDIPYPANYGGVIDVFYKLRALVSAGIKIHLH
                     AFQYGRQSSKILKELCESVHYYQRDVSKSQLFLKEPYIVVSRSSEQLIKNLLSDEHPI
                     LFEGLHSTHYLSDERLKKRKKIVRTHNIEHEYYKNLAKAESNIFKRYYFYNESSKLEI
                     YENILSNADVIATISPNDTQYFKSKYGNAHYIPAFHSFTKVNSRIGKGAYAFYHGNLA
                     IGENDMAALYLVQEVFKNSPVTLLIAGSKPGVNLRDAVQRKNNITLLSDLNTKQINDL
                     VLDAHINVLPTFQPTGIKLKLLSALFSGRFALVNTPMVQNTGLEQLCTVADTPEAMRE
                     ALSAMIKQEFTQIDISLREEILMENFNNKANALKLKNLIF"
     gene            6291..6656
                     /locus_tag="PKU82_03530"
     CDS             6291..6656
                     /locus_tag="PKU82_03530"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOZ81976.1"
                     /translation="MLQVTGILKQKFAEQVVTDKFRKREFTLTIDAKSAYPQIVIFQL
                     ANDKCRLIESFQPGDELSVSFNIKGREWKSPSGEIKYFTSLDAWKIERTSAGESPMES
                     PMPSMSDLPQEDYKDDLPF"
     gene            6717..7508
                     /locus_tag="PKU82_03535"
     CDS             6717..7508
                     /locus_tag="PKU82_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOZ81977.1"
                     /translation="MKNDFKNEKWSLVKPPFRFTNNLKIEVSNFGRVRTSTKIAENRV
                     LKGTLINGYPIVRFKLFKKRADDAQKQLDQKRRKIEQLTGKLRDVKSELKSYKKNSPA
                     YNSNKKKVDNVAAELSTLKEVYRNVFRKDELERTINYAPLVHRLVADSFVKQPSKKHD
                     IVAHLDYNKSNNHFSNLKWMTQEDNIEHQKKSPVVIKEKKKRIGKRPENASGYKLNEK
                     KVAEIKKRIQKGETLSKISDRFNVSETQLLRIKRGINWGDVKVPH"
     gene            7573..8349
                     /locus_tag="PKU82_03540"
     CDS             7573..8349
                     /locus_tag="PKU82_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOZ81978.1"
                     /translation="MQHIFFFLSKILDFLLSPLSWIVILLLISVITKNKKRSRKCLYW
                     AIGAILFFSNPFVLNLVMHAWEIQSVSIDSIHKEYDAAIMMGGAIRYVNGQMQRPVFG
                     SGADRYVQSLELFYRNKAKYIIISSGSGSLVYTDVKEADLLRNQMIRIGVPADKVIAE
                     NQSRNTYENAKNTAEIIRKSDFRPPFLLVTSAFHMRRSMLCFQKQGIDVVPFAVDQHS
                     GQIMFTPDKLFLPSPEVLLDWDLLFHEWFGIAVYKVMGYL"
     gene            complement(8402..8791)
                     /locus_tag="PKU82_03545"
     CDS             complement(8402..8791)
                     /locus_tag="PKU82_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOZ81979.1"
                     /translation="MKAVQKKQKVKMEFPMRSSPKILYGFLSTPSGLSEWFADDVTIR
                     EGVYTFHWDGDEQQAKMTHKRENQMVRFKWIDDSDDAFIEFEITTDELTSDVSLMVSD
                     FAVEDDIEETQRLWDTQIHNLRHLIGS"
     gene            complement(8856..10667)
                     /gene="typA"
                     /locus_tag="PKU82_03550"
     CDS             complement(8856..10667)
                     /gene="typA"
                     /locus_tag="PKU82_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOZ81980.1"
                     /translation="MQNIRNVAIIAHVDHGKTTLVDKILHQTQVFRQNQQTGELILDN
                     NDLERERGITIVAKNVSVQYKDVKINIIDTPGHADFGGEVERVLTMADGVILLVDAFE
                     GPMPQTRFVLQKALALGLKPIVVINKVDKENCRPDEVHESVFDLFFHLEATEEQLDFP
                     TLYGSSKHGWMSTDWRKETSDITALLDAIIEKTPPAKSLEGTPQMLITSLDYSNYVGR
                     IAIGRIVRGTLKENMPVTLMKRDGSAKKSRIKELFVFEGLGRKKVDSVSSGDIAAIVG
                     VDDFEIGDTIADFENPEALKSIAVDEPTMSMLFTINNSPLFGKEGKFVTSRHLRDRLY
                     KETEKNLAMRVEETNSPDAYLVYGRGILHISILVETMRREGYELQLGQPQVLYKEIDG
                     IRCEPVEHLVVDTPSEVAGKVIELATQRKGELTSMLPKGDLQRLEFNIPARGLIGLRN
                     AVLTATAGEAIMAHRFNSYEPAKGTIPGRNMGVLLAKEAGSSTAYSIDKLQERGAFFI
                     DPGETVYGGQVIGEHIRPGDLVVNVCEGKKLTNIRASGSDDNARITPKIKFSLEEAME
                     YIEKDEYLEVTPQNIRMRKIYLDENERKRMSKKAEMA"
     gene            complement(10745..12187)
                     /locus_tag="PKU82_03555"
     CDS             complement(10745..12187)
                     /locus_tag="PKU82_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ81981.1"
                     /translation="MAKLKLSENNLQTEMLEHSLFRQIASLCEKHNLKAFVIGGFVRD
                     SFLKRPSKDIDIVVNGDGIAVAKELAAVLEGEFAFFKNFGTAMVKSEGFEIEFVGARK
                     ESYSQHSRKPKVSPGTLEDDQLRRDFTINTLAFSLNSDDYGKLIDPFNGKQDLNDKII
                     RTPADPVTTYSDDPLRMMRAIRFATQLNFSIEEKSLAAIQSEKSRISIVSKERITDEL
                     NKIIASPLPSTGFKLLMDTGLLELIFPQFYNLKGVDYFEGKGHKDNFYHTLEVLDNIA
                     KVTDNLWLRWAAILHDIAKPATKRFEPGHGWTFHGHEDKGSRMVPPIFRELKLPLNEK
                     MKYVQKLVLLHLRPIVLAQELVTDSAVRRLLFDAGDDIDDLMLLCQADVTTKNEYKKA
                     KYRNNFELVIQKLKEVEEKDRIRNWQPPVTGQLIMDTFGLPPGPNVGIIKDFVREAIL
                     DGDCPNEQQAAFELMLKKGLELGLTPQKKS"
     gene            complement(12159..12746)
                     /locus_tag="PKU82_03560"
     CDS             complement(12159..12746)
                     /locus_tag="PKU82_03560"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOZ81982.1"
                     /translation="MKNTNTEIFNDDIKSCLEILRQGGLIVYPTDTIWGIGCDATNDA
                     AVEKIFKLKNRSESKSLITLVSDEAMLNRCVQNVPAVAWDIIEYSEKPVTIIYEKISR
                     ISEKAIAEDGTAAIRLVKDSFCKMLIHKFGKPIISTSANMSDRPSPAVFNDIEHSILS
                     GVDYVVKYRQQENTKHTASSIIRILNNGEVKVIRK"
     gene            complement(12819..14120)
                     /gene="murF"
                     /locus_tag="PKU82_03565"
     CDS             complement(12819..14120)
                     /gene="murF"
                     /locus_tag="PKU82_03565"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOZ81983.1"
                     /translation="MIAHLHSLFLNSTGVCTDTRKLLKGNIYFALKGPSFDGNKFALQ
                     AIQQGAIAAIVDDSSLKGENIYLVENVLTTLQQLATYHRQVLKIPVIGLTGSNGKTTT
                     KELISRVLGTSYNVLFTEGNLNNHIGVPLTLLRLKSEHQIAVIEMGANHQGEIRELSM
                     IALPNFGLITNIGKAHLEGFGGIEGVIKGKTELYRHLMENDGVIFCRKNNELLTNNLN
                     HYQKVIFYSTQVDNCICGEISTPASVVKFNWFENQNRIDEIITHLTGDYNLENLVAAV
                     CIGRYFNITPDKIKTAIEGYIPENQRSQIEISGSNTIIIDAYNANPSSMEAALKNLSE
                     NYDGNKVIILGEMLELGQDANTEHQHIVDLLSSMEKDLLILVGENFLHTTLNDHALRF
                     KTSEDACNYLHNHAINESTILVKGSRGSKMEKVLDGIRNHN"
     gene            complement(14195..15643)
                     /locus_tag="PKU82_03570"
     CDS             complement(14195..15643)
                     /locus_tag="PKU82_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ81984.1"
                     /translation="MNLRKNAKTLAFVALVVMATNACKQKEKSSTTGWKYNDEKNGGF
                     EVTPYTEQETGPGLILVEGGTFAMGRTEQEIPYDWDNIPRRVTVSSFYMDETEIANVH
                     YLEYLYWLDRVYGTDYPEVYKKALPDTLVWRSKLGYNEPMVELYLRHPAYQRYPVVGV
                     NWLQANDFASWRTDRVNEQILVREGILRVNPAQVNEDNFNTDAYLAGQYEGLVKSKLQ
                     DLNPNRDERNVRMEDGILLPRYELPTEAEWEYAALGLIGNTVYERVTDRKIYPWNGHV
                     VRNSSEKYKGEMLANFKRGRGDMMGTAGALNDNADRPAPVFSYLPNDYGLYNMAGNVS
                     EWVKDVYRPLSPEDMDDFNPYRGNVFVTPLLDEEGQYAEKDSLGRMVWREVDEKENEA
                     RRNYTKADYINDLDGDEPDNIEYKMGGTTMIDDNAHVIKGGSWKDRAYWMSPGTRRFL
                     DQQQSTDWIGFRCSMVRVGSPVGFNQKASKKG"
     gene            complement(15685..16683)
                     /locus_tag="PKU82_03575"
     CDS             complement(15685..16683)
                     /locus_tag="PKU82_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ81985.1"
                     /translation="MIKRIVLTMIISLELAICFGQDPQFSQFYANPIYLNPAFAGSVR
                     CPRLALNYRNQWPGLNKTFITLSASYDQHVEALSGGLGLIIMNDKAGNGTLNTTNFSG
                     IYSYNLNISRNFSIRAGLQATYVQEKLDWDKLTFGDMIDPRYGYVFETQEVRPAEKRG
                     FADFSAGVIGYSSKVYGGFAVHHITEPDQAFIVEGSSPLPRKYTAHVGALLPLKGAST
                     YRGKLEGTFISPNFLYQQQQGFNQLNFGLYVIKSPIVGGLWYRGYTGKKFLSSDSFIA
                     LIGLQKDMFKFGYSYDVTVSKLNNNVTAGSHEISLGLQFECRPKKKRFKAISCPTF"
     gene            16890..20717
                     /gene="porU"
                     /locus_tag="PKU82_03580"
     CDS             16890..20717
                     /gene="porU"
                     /locus_tag="PKU82_03580"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOZ81986.1"
                     /translation="MKRSFALLFLIGLTCVAVAQQDESLKRTYSVDWRSNVVLNGENR
                     LNFTNCFYKPDSKMPFFSTGLPLFDYTKVAEVSIENPVYEPLNINISDEVASEIVPET
                     KLSYFKGKPSLYVEFVPVRKNSVTGQTEKLVAFNLKTTAGQQSQKIGATSTQVYAANS
                     VLASGDWYKISVTEDGVYKITYSFLKDQLKIDLSSSSPQSFRLFGNGGGILPLLNSQF
                     RYDDLQENAVFVNDGGTAGKWDDQDYILFYGQSPVQWLFNSSQNRYYHQANYYSDSTF
                     YFITFNGGTGSPKRIQQSASLSVTPDFVVNSFDDYQYHELDKTNFVKSGREFYGESFD
                     INLNQTFSFTFPNILQQTVTIKTVFAGHSPGVTSSASARYQNITLFSGTYTTGSQYTD
                     DYAMERTTTSTFLPSAGDVVNIDFAFTPANTECIGFLNYIELLAKRALTFAGNGATDQ
                     MFFRSMEASGPGKTAQFNIASAPSSMTVWNVTDRINIRQQLHNNGSFITQVDSTQQFL
                     AFGGNNFLTPGVIGTVANQNLHSLPQADLIIVTHPLFEDQANRIADMHRTLDNMSVNV
                     VRTDLIYNEYSSGAQDIAGIRNFVKMFYDRGIGNGTEPKYLLLFGDGSYDNKYRRANN
                     TNFIPTFESENSYSFLSSFVADDFYGLMDDNEGLCSSNEKIDIGVGRIIAQNISEAKT
                     VTDKTIQYASLPQSSGCPGCDGSGGTLGDWRNVVTFIADDEDGPIHVPPAESFANYLN
                     TYHKTYNVDKIYLDAYKQISTPAGQRYPDVNDAIDKRVNKGSLIINYVGHGGETGWAE
                     ERVLTNDDINSWTNINKLPVFITATCEFSRWDDPARTSSGEKILLSPTGGGVALFSTT
                     RLVFASSNEVLNRALIKFMFDDPNYRLGDIMVASKTDPGNLNLNTRNFSLLGDPALRL
                     HYPKFNIEATQINAQPIIALNDTLSSLEKVTIAGRIVKGGQLQSDFNGFLYPTVYDKA
                     SSITMLRNDASSPNITFKLQKNILYKGKATVKNGEWSFTFIVPKDISYQFGLGKLSFY
                     AENGVEDGAGYFDSIVVGGSSANIISETDGPGIKLYLNDERFAFGGTTNEKPTIVCLL
                     SDSTGINTVGNGVGHDITAIIDGKVDPVYVLNDYYEADLDSYSSGRVAYPLSKLPEGR
                     HTLKVKAWDILNNSSEAYTEFIVASDASLALKHVLNYPNPFTTHTQFSFEYNKPCEDL
                     EVQVQIFTIAGKLVKTISKKIITPGTRIDDLTWDGRDDFGDRIGRGVYIYRVKLNTAD
                     ESASETQKLVILK"
     gene            20844..22046
                     /gene="porV"
                     /locus_tag="PKU82_03585"
     CDS             20844..22046
                     /gene="porV"
                     /locus_tag="PKU82_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOZ81987.1"
                     /translation="MRYKLFYGALSLAVLSVQNQVSAQTDKANLLGQINTITTAVPFL
                     MISPDARAGGMGDAGVASSADVNSIHWNPAKLVFADKKMGVGISYTPWLRKLVPDINL
                     AYVSFFTKPNDVSAIGASLRYFSLGNIDFTSETGDPMGSYKPNEFAVDVAYSRKLGDE
                     FSGGIALRYIHSSLTGGITVGGNATKAGNSVAADVSFFYKTNLEISGKDAEFGAGLAI
                     TNIGSKMSYTDKGTKDFIPINLRLGPSLKIKLNDYNELAFLVDFNKLLVPTPPVYSDS
                     TIAGEENPIIFAGKDPNVGVAQGIFQSFGDAPGGGKEELREINIAGGLEYWYDKQFAF
                     RAGYFYENKTKGNRKYFTLGIGLRYNIFGLDFAYLIPTEQRNPLENTLRFSLSFNFDS
                     IKGSDSNE"
     gene            22050..22529
                     /gene="ispF"
                     /locus_tag="PKU82_03590"
     CDS             22050..22529
                     /gene="ispF"
                     /locus_tag="PKU82_03590"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOZ81988.1"
                     /translation="MNFRVGFGFDVHQLEQGIPFFLGGVRIEHYKGAKGHSDADVIIH
                     AICDALLGAAALDDIGKHFPDTSNEFKGIDSSILLAKVVSLLKEKSIAIGNVDVSVVL
                     EKPKIAPYIQAMREKLASVMQIEIDCVSIKATTNEKMGFVGREEGVAAYAVALINRQ"
     gene            complement(22516..22923)
                     /locus_tag="PKU82_03595"
     CDS             complement(22516..22923)
                     /locus_tag="PKU82_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HOZ81989.1"
                     /translation="MDNRHDIITPDDITLMVDTFYSAIREDDLLGPIFNNIIQDRWPE
                     HLKKMYGFWRSILFNEPLYNGSAFEAHRQLPIEMKHFKHWLKLFEATVDGLFEGSNAE
                     TAKSRARKIAEVFYYKIESERGNEPQNFSPTAG"
     gene            complement(22933..>24142)
                     /locus_tag="PKU82_03600"
     CDS             complement(22933..>24142)
                     /locus_tag="PKU82_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOZ81990.1"
                     /translation="NELVENAPCFKEIAEQLFQLLDGKIFVAHNASFDFSFIKSEFKR
                     CGIDFNAEVICTCSLSRKIFPGFGSYSLGNLTKNLGIILEGHHRAMNDAVAASEILEK
                     LVSEKGLEHTLLHRSVPIKNTEIPHELISKLPETPGIYFFTDSNHEILYIGKAKNIKK
                     RVTSHFRNNNSKSKKIKALLHDISYMNTGCELLACLLEIEYIKEYQPFFNRAGKSEKN
                     FGVYKNDILNGLSKFFIHDISKTNSSPLRYFGSRKAADEFLSHITEKYNLCRCINSLE
                     SYSDNCFYKQIKQCNGIVLDVNEKANYNNRFNDACLEVGDGWHDAALAYPIAGSEEWA
                     YVFRHQGAIGYYFGKKATTFNINLIKGVTHLKNHLESQSIFNSFLKKPSAKKIKIISG
                     EIREVEIEKK"
BASE COUNT         7448 a         4322 c         4395 g         7877 t
ORIGIN      
        1 attcagtaaa cagttaggaa tgattggcag ggaaacattg tttatggctt atgatgggtt
       61 gcattctgct aaggtcagtg cagcaaaagc actaaagagt gctatcgtaa gtgtatctat
      121 gtctgatgtt tcggaaatta aaaaacacaa tggaaatttt atattgaata ccggctcacc
      181 gcattacgta aagtttgttg atgatacaga taaaattgat gtcgttaaag aaggaaaaaa
      241 aataagaaac agcaatactt ttaaggcaaa gggcattaat gtgaattttg tttcatttaa
      301 aagcaaagca tcaaacatca gaacttatga acgtggggta gaggacgaaa ctcttgcctg
      361 tggcaccggc ataactgcat ctgcaatagc agcacattat tccggtttaa caaagaaatt
      421 atcggtgaat gtgattgcac gtggtggaag tctgaatgtt actttccagg ttgaagctaa
      481 caaatacaaa aatgttatgc taacaggttc tgctatgaat gtttttgatg gtgaaattaa
      541 aatctgaatt tagcagttag catggtatgt gttcctgctt gaggatagta gaaattttcg
      601 ttaaccctca taccatcata taagtaccca taagtataac cattacttga atacatttca
      661 ttgaacaggt tatatactgc aactcctgct tcagtttcaa tggttttaaa aatcttaaac
      721 gtataatgta ttgaaagatt ggtgtaggca aatgctttca atgatttttc ctccgaggag
      781 gtattatcta aatattgttt tccaacatac ttctcagtta gtttgatgct taacattttt
      841 aacggcctta tttcaatacc gacagcagta attattgaag gagaaaaagc aatgtctgtt
      901 tttttataga taattttctg ctgacctgta tagttaaaat ctgcatcgta ctgatcaata
      961 tattccttaa agttcagaat tttattttcg cttaatgcta cattgcctga aacagctaag
     1021 aacttaccca catcataatt aaattctgct tctaaacctc ttctgaaact attttttacg
     1081 ttttgtctgg tatagttgcc aacgtcattg atttttcctg ttaaaatcag ttggtcttta
     1141 tattgcatat aaaaggcatt aagactcagt tttaattttt ttattgcgat tgaataacct
     1201 gcttcaaaat ctatgagatt ttctgattta ggtcgtgatt ttgcagttga ctcagtgaaa
     1261 tcatctcttg atggttcttt gtttccaaca gcgatagaca gccaggcact ttcgttttca
     1321 ttacgtaaaa aagtaatgcc ggcttttgga ttgaaaaaat gaaactctgc aacctgatta
     1381 gtggtggtgg caaaatcatc aaatcctgta aaccgataat gtacgtagcg atattgaatg
     1441 tctgcatatg caatgaaatg ctcccccaag tgtaaggttc cttttatcca gtgattgaaa
     1501 ttgtttttta atccattatt ttgatagtaa gtgtaatttt ctgaagctaa tggaataatg
     1561 tcacctttaa ttattttacc gtagtgatca ccatcatatt gattgatgcc acctccggtt
     1621 tttatttcaa ggtttttatt ttcataagtc aaagagtaaa tcaagccata aaaataattg
     1681 tcgagccact ttcttctcac aagatttgtt ttagtaatta tggtatcctg aatttgaata
     1741 ggaggtaatc catagtcact aaactttgca ttttttttaa gctcctcata atagccttta
     1801 ccacgtgtca ggtgagcagc aacactcaaa gtaaacaggt tgttaatctg atgtgagaag
     1861 tgcaactgat aattgtcctg ttggtaattg tctgtttgtc cgggatataa atagtaattg
     1921 tatttccttc ctgattcaag aaggtttgta gcgtcttcac tgtctaagcc attacgggaa
     1981 atataatcca tcattccctg catgttattt ttataccttg actccggcac accataccat
     2041 gactgataag tcgtttctat tcctgaaaaa atgattgcct tcaacattga ctttttacca
     2101 aaatatcctc ccgaaaaata aaaagatttt aaatttgatg atgctctgtc aacatatccg
     2161 tcagactgta tggaagatag tctggtttca aaagcaaagc gattattcag taatccggtg
     2221 cctgcaataa gattcacctt ttttgtattg aaagctccgt aactcaagtt tgtttctgca
     2281 aatggttttt ctgaaagatt gtctgtaaga atattgatgc ttgcaccaaa tgctgctgcg
     2341 ccatttactg aagtgcctac acctctctga agttgtatct gattggttga gctaagcagg
     2401 tcaggcatat caacccaata catactttgc gactccgcat cattaattgg aataccattt
     2461 atagtaacat taattcttgt ggcatcacta ccacgcacgc gcattcctgt gtagccaaca
     2521 cctgcacctg catcactgct aaccacgaca cttgtgagtt gatccataat ttccggcaca
     2581 tctttccctg aattcatttc ttcaatttca tttttattca gactgttttg tgcaataggt
     2641 attttgtttt tcatttttaa tgactgaata attacctcgt cagttacaaa tgcttttttg
     2701 aaaagtatta cttctatatg ttgattactt gccaggttaa tttcactgct gtattcatga
     2761 tagccaagct gatttacctg caaagtgtat ttacccggtt taagattaaa aaaagtaaac
     2821 ttgccttttt catctgaaaa atgacgtatc agcgattctt taagggtaac aactgcatga
     2881 tccacttttt taccatcatt agtactcaca actcctgata gatcgagctg tgcattagaa
     2941 tagaggctta agcataacgc taaaagcaat aaagaaaatt tcttaatcat catttttcat
     3001 ttttaagtaa catcacaaaa gaagttgcag gtgcaataaa agaatgttac ctatccctac
     3061 gcaggcatta tcctgatcag gttatgggta taatctcagc attcctttca ctacaggaag
     3121 cacccctttt gatgatgcaa agtaatcatt cattttccga aatcacaaaa aatcctgtaa
     3181 tgactttttt cagtttgttg aaatcttttt aaacaaatga tgcattttgg ttgaatgttt
     3241 ttttggatta atgatttttt tactattaac tttgtaagat aaaccgaaac aatttatgag
     3301 acagctgcgc attcaaaagt ccataaccaa cagagaaagc caatcgttgg aaaagtacct
     3361 tcaggaaatt agccgcgaag gcatgattac tgcagaggaa gaagctaatt tagctcgaag
     3421 aattcgtgag ggtgatgagg aagcattgag taaattaaca agagctaatt tgagatttgt
     3481 tgtgtcagtt gccaagcaat atcagaatca gggattaagt cttcccgatt tgataaatga
     3541 aggcaatctc ggacttatta aagctgcatc acgttttgat gaaaccagag gatttaaatt
     3601 tatttcttat gctgtttggt ggatacgtca aagtattttg caagccattg ctgagcaggc
     3661 acgcattgtg aggcttcctt taaatcaggt tggttcgtta agaaaaataa ataaagtttt
     3721 ttcaaaattg gaacaagagt ttgagcgtga accctcagct gaggaaatag cagagatgat
     3781 ggaagtaccg attgagaaaa tttccgacac attaaaatct cctggtaagc atgtcagtat
     3841 ggatgcacct tttgccggca atgaagaggc tactttactt gatgttatgc aaaatgatga
     3901 tgtacccgat gcagattttg gcctcatgaa tgaatcactt cgtaaagaga ttgatcgttc
     3961 tttaaagaca cttacagagc gtgaacgtga tgtggttaaa ttgttttatg gcattggtgt
     4021 ttcacatagt ttttcattgg atgaaatagg ggctaagttt ggtttaacaa gagaacgggt
     4081 caggcaaata aaggagaaag ctataagaag attgcgctca ggctctcgaa acaagttgct
     4141 taaagattat ttagcctgat ttaaatttaa caagcaaagt tttaccttcg ccacatcaac
     4201 taagaatggc gcaaaagaag gttataattt ctgtaattaa tgatatagca accgatcagc
     4261 gggttagaag aacagcatca gtattttctg agcttaacta tcaaatactt attgttggtc
     4321 gaaagttacc acttagttta cctgtttatg atatgaccgg tttnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnntatggta atgatgtgaa agttgttcgt aatgttcctg
     4501 atagaacaga tagcgttaat ttcgatgtag cgaaatggaa gcgttctgtc ggatttcctg
     4561 attacaagaa ggtctttata atgcaaggtg caggaattaa tgttgacaga ggcgcagagg
     4621 aggcaattga agcaatgaaa gatgtagatg ccatgttact tattataggt gggggagatg
     4681 tttttcatca gttacaagaa ttggctatca aacataatgt aaaacacaaa gtgattattc
     4741 accctaaaat ttcacgcgag gaattaatta ctgtaacagc atcatgcttt gcaggactta
     4801 cattggataa agacaccaac atcaattata aattcagttt gccaaacaaa ctattcgatt
     4861 acattcatgc aggcattcct gttatagctt cagatttacc tgaggttaca gcaattgtga
     4921 acggatataa aacggggatt gtgttgccac aatactctcc acaagcaata gcagaggcaa
     4981 tgaatcgttt agttatcgaa aaagaacttt atgttagcta taaagaaaat tgtagtattg
     5041 catctaagga tttatgctgg caaaaagaga aaaaaatatt aattgatgta attttgtcct
     5101 ctgagaaata gccaaacaaa ctttaaaaca cattgtgaag aacagcggta agcatttaca
     5161 tattatcagt tttgatattc catatcctgc aaactatggt ggagtcattg atgtgtttta
     5221 taagctccgt gctcttgttt ctgccggaat aaaaattcac cttcatgcat ttcaatatgg
     5281 tcgtcagtca tcaaaaattc ttaaagagtt atgtgaatct gtccactact atcaacgtga
     5341 tgtttccaaa tcgcagttgt ttttaaaaga accatatatt gttgtgtcac gctcgtcaga
     5401 gcaactgatt aaaaacctgc tttccgatga gcatccaata ctatttgaag gattacatag
     5461 tactcattat ttgtctgatg agcgactaaa aaagcgaaag aaaatagtca ggacacataa
     5521 tatagaacat gaatattata aaaacttagc aaaggcagaa tcaaacatct ttaagagata
     5581 ctatttttat aatgaatcga gtaagttaga aatctacgaa aacattttat caaatgcaga
     5641 tgtgattgcc accatttcgc ctaacgacac acaatatttt aaaagtaagt atggcaatgc
     5701 acattacata cctgcttttc attcctttac aaaagtaaat tcgagaattg gcaaaggagc
     5761 ttatgccttt tatcacggca atctggctat tggtgagaat gatatggctg ctctctattt
     5821 agtacaagaa gtttttaaaa actcaccggt taccttgctg attgccggta gcaaacccgg
     5881 tgttaattta agagatgctg tacaaagaaa gaataatatt actttgctaa gtgatttaaa
     5941 cactaagcag attaacgatt tagttttgga tgctcatata aatgtgctgc ccacttttca
     6001 gccaacagga attaagctta aactgctttc agcacttttt agtggtcgtt ttgccctcgt
     6061 aaatacacca atggttcaaa atacaggatt ggaacagttg tgcactgtag ccgatacacc
     6121 tgaagctatg cgtgaggcac tttcagcaat gattaaacag gaatttactc agatagatat
     6181 ttcgctacgg gaagaaattc ttatggaaaa cttcaataat aaggcaaatg ctttaaaact
     6241 taaaaatttg attttttaaa tttattttta cttttgaaaa aaaattcaaa atgcttcagg
     6301 taacaggaat attaaaacag aaatttgcag aacaggtagt aacagacaaa tttcgcaaaa
     6361 gagaatttac acttacaata gatgctaagt cagcctatcc tcaaatagtt atttttcaac
     6421 tggcaaatga taagtgcagg ttaatagaga gttttcagcc gggtgacgag ctttcggttt
     6481 ccttcaatat aaaaggcaga gagtggaaat caccatccgg tgagattaag tattttacat
     6541 cgctcgatgc atggaaaatt gaaagaactt ctgcaggaga gtccccaatg gaatcgccaa
     6601 tgccatctat gtcagattta ccacaggaag attataaaga cgatttgcca ttttaatcag
     6661 ttaaatttat tgaatcatta aatcacaatt gaattctaat tgtaaatttt caaacaatga
     6721 aaaatgattt taagaatgaa aagtggtcgc ttgtaaagcc tccgtttagg tttaccaata
     6781 atctgaaaat agaagtatca aattttggga gggtcagaac ttccaccaaa attgcagaaa
     6841 acagagtgct gaaaggaact ttgataaatg gatatccgat tgtgaggttt aagcttttta
     6901 agaaaagagc tgatgatgca caaaagcaat tggaccaaaa acgaagaaaa attgaacaac
     6961 taacaggtaa actgcgcgat gtaaaatcag aattaaaatc ttacaaaaag aattcacctg
     7021 cctataacag caataaaaag aaagttgata atgttgctgc agagttgagc accttaaagg
     7081 aagtgtatag aaatgtattc cgaaaagatg aattagagcg cacaattaac tatgctccat
     7141 tggtgcatcg tcttgttgca gatagtttcg ttaagcaacc ttctaaaaaa catgatattg
     7201 ttgcacatct tgattataac aaaagcaata atcatttttc aaacctgaag tggatgactc
     7261 aggaagataa tatcgaacat cagaagaaaa gtcccgtagt tataaaagag aagaagaagc
     7321 gaattggtaa gcgtccggaa aatgcatcgg gctataaact caatgagaag aaagtagctg
     7381 aaataaagaa acgaattcaa aaaggagaga cattatctaa aatatctgac cgatttaatg
     7441 tttcggaaac gcaactactt cgaattaaga gaggaataaa ctggggcgat gtaaaagttc
     7501 cccattaagt ttactcatct gtctgatttt tgtttactca tctgtctgat ttttttgttg
     7561 taacttgatt taatgcaaca catatttttt ttcctttcaa agattttaga ttttttatta
     7621 tcgccactaa gttggatcgt aatcttgttg cttatttctg ttattacaaa gaataagaaa
     7681 cgaagccgga agtgtttata ttgggcaata ggagcaatac tatttttcag caatccgttt
     7741 gtgctgaatt tagtaatgca tgcatgggaa attcagtcgg tatctataga ttcaattcat
     7801 aaggaatatg atgcggcaat aatgatgggt ggtgctatac gttatgtaaa cggacagatg
     7861 caacgtccgg tttttggttc aggtgcggat agatatgttc agtcgctgga gcttttttat
     7921 agaaacaaag caaagtatat aattatttcc agtggttcgg gcagtttggt ttatactgat
     7981 gttaaagaag cagatttact gcgtaatcag atgatacgca taggtgttcc tgcagataaa
     8041 gtgattgcag aaaatcagtc aagaaacact tatgaaaatg cgaaaaatac tgctgaaatt
     8101 attcgaaaat cagattttag accaccattt ttattagtga catcggcatt tcatatgcgg
     8161 aggtcaatgc tttgttttca gaaacaagga attgatgtgg tgccttttgc agttgaccaa
     8221 cattcaggtc agataatgtt tacacctgat aaactttttt tgccatcacc ggaggtgtta
     8281 cttgattggg acttgctatt tcatgaatgg tttggaatag cagtttataa agtaatgggg
     8341 tatttgtaag ataaatatta agtctaatat caaaacaatg tatagagata ttagtgtaac
     8401 actatgatcc aatcagatga cgcaaattat ggatttgtgt gtcccataaa cgttgtgttt
     8461 cttcaatatc atcttctact gcaaagtcag ataccattag tgatacatca gatgtcaatt
     8521 catcggttgt aatttcaaat tcgataaaag catcatcgct gtcatcaatc catttgaaac
     8581 gcaccatttg attttctctt ttgtgagtca ttttggcttg ctgctcatcg ccatcccaat
     8641 ggaaagtgta aacaccttca cgaatagtga catcatcggc aaaccattca gatagccctg
     8701 atggagtgga taggaatccg tataatattt tgggagaaga ccgcattgga aactccattt
     8761 ttactttttg ctttttctgc actgccttca ttgtatgtta ttgtttaatt tttacaataa
     8821 taataaaaaa acttatacaa tcctaactta tttcactatg ccatttctgc tttcttactc
     8881 atgcgcttac gctcattttc atcaagataa attttacgca tgcgaatatt ctgaggagta
     8941 acttcaagat actcatcttt ctcaatatac tccattgctt cctctaacga gaattttatt
     9001 tttggagtaa tacgcgcatt gtcatcactg cctgatgctc tgatgttggt taattttttt
     9061 ccttcacata cattcaccac caagtcgccc ggacggatat gttcaccgat aacttgtcca
     9121 ccataaactg tttcacccgg atcaataaaa aatgctccac gctcttgtaa tttatcaata
     9181 gagtaggctg ttgaacttcc tgcttcttta gcaagcagca cacccatatt tcttccggga
     9241 atagtaccct tagcaggctc ataagaattg aaacgatgtg ccataattgc ttctcctgca
     9301 gttgcagtaa gtactgcatt tcttaaacca attaatcctc ttgcaggaat attaaattca
     9361 agtctttgta aatcaccttt aggcaacatg cttgtaagtt ctcctttacg ctgtgtggca
     9421 agttcaatca ctttacctgc tacttctgat ggtgtatcaa caactaaatg ctctaccggc
     9481 tcgcaacgaa taccatctat ttctttgtac aaaacctgtg gctgtcctaa ctgaagttca
     9541 tagccttctc tgcgcatggt ttcaacaaga atagaaatgt gcagaatacc acgtccataa
     9601 acaagatatg catccggtga attggtttct tcaacacgca tggccagatt cttttctgtt
     9661 tctttataaa gacgatcacg aaggtggcga gaggtaacaa actttccttc tttaccaaac
     9721 agcggactgt tgttgatggt aaaaagcata ctcattgtgg gttcatcaac tgcaattgat
     9781 ttcaatgctt cagggttttc aaaatctgca atggtatcac cgatttcaaa atcatctaca
     9841 cctacaatag ctgcaatgtc gccactgctt acgctgtcaa ctttttttct gcctaatcct
     9901 tcaaaaacaa acagttcttt tattcttgat ttttttgcac taccatcacg tttcatcaac
     9961 gtcacaggca tattttcttt caatgttcct ctcacaattc tgccaatggc aatacggcca
    10021 acatagttgc tgtaatctaa cgatgtgatg agcatctgag gtgtgccttc aagcgatttt
    10081 gcaggaggcg ttttctctat aatggcatca agtaaagctg ttatgtctga tgtttctttg
    10141 cgccagtctg tgctcatcca accatgttta ctactaccat ataatgttgg aaagtcaagt
    10201 tgctcttctg ttgcttcaag atgaaaaaac aaatcaaata ccgattcatg tacttcatct
    10261 gggcgacagt tttctttatc aactttgttg atgacaacga ttggcttaag tcctaatgca
    10321 agtgctttct gcaaaacaaa gcgtgtctgt ggcattggtc cttcaaaagc atctactaaa
    10381 agaattacac cgtcagccat tgtaagaaca cgttctactt ctcctccaaa gtcagcgtga
    10441 cccggtgtgt caataatatt aatttttacg tctttgtact gaacagatac attttttgca
    10501 acaatggtaa ttcctctctc acgttccaga tcattattgt cgagaatcaa ttctcctgtt
    10561 tgctgatttt gtctgaaaac ctgtgtctga tgaagtattt tatcaactaa ggttgtttta
    10621 ccatggtcaa cgtgggcaat aatggccaca tttctgatat tttgcatgaa aattttattt
    10681 ggggtgcaaa gataagactt tttaccgccc aaatattgat tgtcaataat aaaagtagaa
    10741 aaaatcagct ctttttttgt ggtgttagcc caagttcgag gcctttttta agcattaact
    10801 caaatgctgc ctgttgttca ttaggacaat cgccatctaa aattgcttcg cgaacaaaat
    10861 cttttataat accaacatta ggtccaggcg gcaaaccaaa tgtatccata atcaattgtc
    10921 cggtaacagg tggttgccag tttctgattc tgtctttctc ttcaacttct ttaagttttt
    10981 gaatgaccaa ctcaaagttg ttacggtatt tggctttctt atattcattt ttagttgtca
    11041 catcagcctg gcacaacaac atcaggtcgt caatatcatc accggcatca aataacagtc
    11101 tgcgtactgc tgaatcggta acaagttcct gtgccaaaac aatgggtctt aaatggagca
    11161 aaaccaattt ttgtacatac ttcatctttt cattgagagg taattttaac tctctgaaga
    11221 taggaggaac catacgtgaa cctttgtctt catgcccatg aaaagtccat ccatgcccgg
    11281 gttcaaaacg ttttgttgca ggttttgcaa tgtcatgcag aatagcagcc catcgtaacc
    11341 ataaattatc agtgactttt gcaatgttgt ccaatacttc caaggtgtga tagaagttat
    11401 ctttatgacc tttgccttca aaataatcaa ctcctttcag gttgtaaaac tgcggaaata
    11461 ttaactccaa caatcctgta tccatcaaca atttgaatcc tgttgatggc aatggcgaag
    11521 caataatttt attcagttca tcggttattc gttctttgga aactatgctg attctactct
    11581 tttcactttg aattgcagcc agcgattttt cttcaatgct gaaattaagt tgtgttgcaa
    11641 agcgtattgc acgcatcatg cgcagtggat catcactgta agttgttaca gggtcggcag
    11701 gtgtgcgaat aattttatcg ttcagatcct gcttgccgtt aaatgggtca atcagctttc
    11761 cataatcatc actgttaaga ctaaaagcaa gtgtgttgat agtaaaatcg cgtctaagct
    11821 gatcgtcttc taatgtgcca ggtgaaacct tcggctttcg cgaatgttga ctatacgact
    11881 cttttcttgc acctacaaat tcaatttcaa atccttcgga ttttaccatt gcagtaccaa
    11941 agtttttaaa gaatgcaaat tctccttcaa gaactgcagc aagttcttta gctacggcaa
    12001 tgccgtcacc gttaactaca atatcaatat cttttgaagg tctttttaaa aaactatcac
    12061 gcacaaatcc tccaatgaca aatgctttta aattatgctt ttcgcaaagt gaagcaatct
    12121 gccggaacaa actatgttcc agcatttctg tctgcagatt attttctgat aactttaact
    12181 tcgccattgt tcaaaatcct gatgatggat gatgctgtat gttttgtgtt ttcttgttgt
    12241 ctgtacttta caacatagtc aactcctgaa agtatggaat gttcaatgtc attaaaaaca
    12301 gcaggggagg gcctgtcgct catgtttgca gaagtagata tgattggttt cccaaattta
    12361 tgaatcagca ttttgcaaaa tgaatctttc actaatctga ttgctgcggt gccgtcttct
    12421 gcaattgctt tttcagagat acgactgatc ttttcataaa ttatagttac aggcttttcg
    12481 gaatattcaa tgatgtccca tgcaacagcc ggaacatttt gcacacaacg attcagcatt
    12541 gcctcatcgc tcactaatgt gattaaactt tttgactcgc tccggttttt aagtttaaaa
    12601 attttttcta ctgcagcatc gtttgtagca tcacatccaa ttccccaaat tgtgtcagta
    12661 ggataaacaa taagtcctcc ttgtcttaaa atttcaagac aagatttaat gtcatcatta
    12721 aaaatttcgg tatttgtatt tttcatcaca atgcaaagaa aacagattga aacgaatttt
    12781 tactttttaa ttcttgagct aatgattttt taaaaaaatc agttgtgatt gcggatgcca
    12841 tcaagcactt tttccatttt agaaccgcga ctgcctttaa ctaaaatagt gctttcatta
    12901 atagcatgat tatgtaaata gttgcaagca tcttcggagg ttttaaagcg caatgcatga
    12961 tcattgagtg ttgtatgcag aaaattttca cctactaaaa taagtaagtc tttttccata
    13021 ctactcaata agtcaacaat gtgttggtgc tctgtatttg catcctgccc aagttcaagc
    13081 atttctccaa gaatgataac tttattgcca tcataatttt cagaaagatt ttttaatgca
    13141 gcttccatac ttgaaggatt ggcattgtat gcatcaataa taattgtatt acttcctgag
    13201 atttctattt gcgaacgctg attttcgggg atgtaacctt caattgctgt cttaatttta
    13261 tcaggtgtaa tattaaaata tctaccaatg caaactgccg ctacaagatt ttcaagatta
    13321 tagtcaccgg taaggtgcgt tattatttca tcaatcctat tttgattttc gaaccaatta
    13381 aatttaacaa ctgatgcagg agtagaaatt tcaccacaga tacaattgtc cacttgcgta
    13441 ctgtagaaaa ttaccttttg ataatgattg agattatttg ttagcaattc attgttttta
    13501 cggcagaaaa ttactccatc attttccatc aggtgtctgt acaattctgt ttttccttta
    13561 attacacctt caatacctcc aaaaccttcg agatgtgctt tgccaatatt tgtaatgagc
    13621 ccgaagttgg gcagagcaat cattgacagt tcacgaattt ctccctgatg attggctccc
    13681 atttcaataa ctgcaatctg atgttcgctt tttaatctta acaaggtaag tggaacaccg
    13741 atgtgattat tcagatttcc ctcagtaaat aaaacgttat atgacgtgcc gagtaccctg
    13801 ctgattaatt cttttgtagt agtttttcca ttgctacctg ttaatccaat aaccggtatt
    13861 tttaaaactt gcctgtgata ggtagctaac tgttgcaaag tcgttagaac attctctact
    13921 aaataaatgt tttcaccctt tagtgaggaa tcatccacta ttgctgctat ggcaccttgt
    13981 tgtattgctt gtagtgcgaa tttatttcca tcaaaagaag gtccctttaa agcaaaataa
    14041 atatttcctt tgagcagttt ccttgtatct gtacaaactc cggttgagtt caggaataaa
    14101 ctatgtaaat gtgctatcat ttatattcaa taactttcaa aaagaaagcc ccaaaaaaag
    14161 gggcttatgt attttattta aatgttgaag gatattatcc tttcttagac gctttttgat
    14221 taaaaccaac agggctacct acacgtacca ttgaacaacg gaatccaatc cagtcagttg
    14281 attgttgttg gtctaaaaat cttcttgtac cgggtgacat ccaatatgca cggtctttcc
    14341 aggaaccacc tttaataacg tgtgcattat catcaatcat cgtagtacca cccattttat
    14401 attcgatgtt atcaggttca tcaccatcaa gatcgtttat gtaatcggct ttagtataat
    14461 ttctgcgagc ttcattttct ttttcatcta cctcgcgcca taccatgcga ccaagactat
    14521 ccttttctgc atattgtcct tcttcgtcaa gtaatggtgt tacaaatacg tttcctctgt
    14581 aaggattaaa gtcatccatg tcttcaggtg ataaggggcg ataaacatct tttacccatt
    14641 ccgaaacgtt acctgccata ttatataaac cataatcatt tggtaaatat gaaaatactg
    14701 gagccggacg gtctgcattg tcatttaatg cacctgctgt tcccatcata tcaccacgac
    14761 ctctcttgaa gttggcaagc atttcacctt tatatttttc tgaagaattt cttactacgt
    14821 gaccattcca agggtatatt tttctgtcag taacacgctc ataaaccgta ttgccaatta
    14881 agcctaaggc tgcatattcc cattctgctt ctgttggaag ttcgtagcgt ggtaataaaa
    14941 ttccatcttc catacgcaca ttacgttcat cacgattggg gtttaaatcc tgaagtttgc
    15001 ttttcaccaa accttcgtac tgtccggcaa gatatgcatc agtattaaag ttatcttcgt
    15061 ttacctgtgc agggttaaca cgtaaaattc cttcgcgcac taatatctgc tcatttacac
    15121 gatcggtacg ccaacttgca aaatcattgg cttgtaacca atttacacca acaacaggat
    15181 agcgctgata ggcagggtga cgtaaatata actcaaccat tggttcgtta taacctaatt
    15241 tacttctcca aactaatgtg tcaggaagcg cttttttata cacttcagga tagtcagttc
    15301 cataaacacg gtctaaccaa tagagatatt ctaaataatg tacattggca atttctgttt
    15361 cgtccatgta aaaagatgaa acagtaactc tgcgaggaat gttatcccag tcataaggaa
    15421 tttcttgctc tgtacggccc attgcaaatg ttccaccttc tacaaggatt aatcccggac
    15481 ctgtttcctg ttcggtatat ggagtaactt caaatccacc attcttttcg tcattgtact
    15541 tccatcctgt ggttgaagat ttctcttttt gcttgcaagc atttgttgcc atcactacta
    15601 aagcaacgaa ggcaagggtt tttgcatttt tacggagatt catttttacg tatttggaaa
    15661 ctttatatgg tttatgtcat taaactaaaa tgtggggcaa cttattgcct tgaaccgttt
    15721 tttctttgga cggcattcaa actgcaaacc taaagaaatt tcatgagatc cggcagtaac
    15781 attattattt agtttagaaa cggtaacatc ataggaataa ccgaacttaa acatgtcttt
    15841 ttgtaacccg atgagggcaa tgaagctgtc gcttgacagg aattttttac ctgtatagcc
    15901 cctgtaccac aaaccaccaa caattggcga ctttataaca taaagcccaa agttgagttg
    15961 gttaaatcct tgctgttgct gatacaaaaa gtttggtgag ataaatgtac cttctaattt
    16021 ccctctgtaa gttgaagcgc cttttaatgg taataatgcc cctacgtgtg cagtatattt
    16081 tcttggtaac ggacttgagc cttcaacaat aaatgcctga tcaggttctg taatatgatg
    16141 aacagcaaaa ccgccatata cttttgaact gtaacctata acgcctgctg aaaaatcagc
    16201 aaaaccacgc ttttcggcag gacgtacttc ctgcgtttcg aacacataac catagcgggg
    16261 atcaatcata tcgccaaagg tcagtttatc ccagtctaat ttttcctgaa cataggtagc
    16321 ttgcagccct gcacgaatag agaaatttcg tgaaatgttt aagttgtaag aatagatgcc
    16381 ggagaagttg gttgtattca gtgttccatt acctgcttta tcattcataa tgattaagcc
    16441 taatccaccg gagagtgctt caacatgttg gtcataagag gcgctcaaag tgatgaaggt
    16501 tttatttaaa ccaggccact gattacgata gttcaatgcc agtcgtggac aacgaaccga
    16561 accggcaaag gcaggattca gataaatcgg attggcataa aactgcgaaa actgcgggtc
    16621 ctggccaaaa caaatagcta actccaggct gataatcatg gtaagtacaa tacgcttaat
    16681 catagttcat ttacttttca aacttaattt tatacgtata gtactaaaat agttacaatt
    16741 ctgcacgaat atattggtgg ttaaaaattt aatcggaaca aagttaggca ttaattattt
    16801 taatatcaaa caaaataaaa ttcaatattg catgtttcat acatgttgta ttgttcgaga
    16861 agcatatttt tgaacccaat taccaaaaca tgaaaaggtc ttttgcacta ctttttttaa
    16921 taggattgac ttgtgttgct gttgcgcaac aagatgaatc tttaaaacgc acttattccg
    16981 ttgattggcg cagtaatgtt gtcctaaatg gcgaaaacag gcttaatttt accaactgtt
    17041 tttataagcc ggacagcaag atgccatttt tctctaccgg acttccacta tttgattata
    17101 ctaaagtggc tgaagttagc attgaaaatc ctgtttatga accattaaat attaatattt
    17161 ctgatgaagt tgcatcggaa atagttccgg aaacaaaact ttcttacttt aagggaaaac
    17221 catctttgta tgtagaattt gtgccggtaa gaaaaaattc agttacgggg caaacagaaa
    17281 aattagttgc atttaatctt aaaacgacag ccgggcaaca gagtcaaaaa attggtgcca
    17341 cttcaactca ggtttacgct gctaattcag ttcttgcttc gggtgattgg tataaaattt
    17401 cagttacaga agatggagtt tataaaataa catacagttt tctgaaagac cagttaaaaa
    17461 ttgatttatc atcttcctca cctcagtcat tcaggctctt tggcaatggt ggaggcattt
    17521 tacctctatt aaactctcag tttagatatg atgacttgca ggaaaatgct gtgtttgtaa
    17581 atgatggagg tacagcagga aaatgggatg atcaggatta tattttgttt tatggacagt
    17641 cacccgtgca atggcttttt aattcctctc agaacagata ttaccatcag gcaaattatt
    17701 attccgattc cacattttat tttattacgt ttaatggagg aaccggaagt cctaaaagaa
    17761 ttcagcaatc ggcatcatta tcagtgacac ctgactttgt tgtaaacagt tttgatgatt
    17821 accaatatca tgaattggat aaaacaaatt ttgtcaaatc tggacgtgaa ttttatggcg
    17881 agtcattcga cataaacctt aatcagactt tttcattcac atttccaaat atactgcaac
    17941 aaactgtgac tataaaaaca gtatttgcag gtcattcacc tggtgttaca agttcggctt
    18001 cggcacgcta tcaaaatata acattatttt cgggtacata cacaaccggt tcacaatata
    18061 cagatgatta tgcaatggaa cgtacaacaa cttcaacgtt cttgccgtct gccggtgatg
    18121 tagtgaatat tgattttgct tttactcctg caaatacaga gtgtattggc tttttgaact
    18181 atatagaatt attagcaaag cgagcactaa catttgcagg taatggtgct accgatcaga
    18241 tgtttttccg aagtatggaa gcttccggcc ccggtaaaac tgcacagttt aatattgcat
    18301 cggcaccatc atctatgacg gtttggaatg tcactgacag aataaatatc agacaacaat
    18361 tacataataa tggttcgttt attacacagg tggacagtac acagcaattt ttagcttttg
    18421 gtgggaataa ttttttaacg cccggagtaa ttggtacagt ggcaaatcaa aatttacatt
    18481 cattgccaca agccgattta atcattgtaa cacatccatt atttgaagat caggcaaacc
    18541 gcatagcaga tatgcacaga acattggaca acatgtctgt gaatgttgtg agaacagatt
    18601 taatatacaa cgaatactct tcaggagcac aggatattgc aggcatacgc aattttgtaa
    18661 aaatgtttta tgacagaggg ataggcaatg gaacagaacc aaaatattta cttttatttg
    18721 gtgatggttc ttatgacaac aaataccgca gagccaataa caccaatttt attccaacct
    18781 ttgagtctga aaactcatat tcatttttaa gctcattcgt tgctgacgat ttttatggtt
    18841 tgatggatga caatgaaggt ttgtgttcaa gcaatgaaaa aattgatatt ggtgtaggtc
    18901 gaattattgc acaaaacatc agcgaagcta aaacggttac agataaaacc attcaatatg
    18961 catcattgcc acagagtagt ggttgtccgg gttgtgatgg tagtggtgga acattaggtg
    19021 actggcgcaa tgtggtaacg tttattgcag atgatgagga tggacctata cacgtaccac
    19081 ctgcagaatc gtttgccaat tatttaaata catatcataa aacatataac gttgataaaa
    19141 tttatttaga tgcctacaaa caaatatcaa cccctgcagg tcagcgttat cctgatgtta
    19201 atgacgctat tgataaacgt gtcaataaag gttcgttgat tattaattat gtaggtcatg
    19261 gtggcgaaac aggatgggca gaagagcgag ttcttaccaa tgatgatatt aactcatgga
    19321 caaacattaa taaactgccg gtatttatta cagcaacctg tgagtttagc agatgggacg
    19381 atcctgcaag aacatcatct ggcgaaaaaa tattattgag cccaaccggt ggaggtgttg
    19441 cattgttttc aactacacga ctggtatttg caagcagcaa cgaagttctt aacagagcct
    19501 tgataaagtt tatgtttgat gatccaaatt atcgtttagg cgatatcatg gttgccagta
    19561 aaaccgatcc gggaaatttg aatctgaata cacgtaattt tagtttactc ggtgatccgg
    19621 cattaagatt acattatcct aaattcaata ttgaggctac acaaattaac gctcaaccaa
    19681 ttatagcgct aaacgatact ttaagctcat tagagaaagt aaccattgca ggaagaatag
    19741 ttaaaggtgg acaacttcag tctgatttta atgggttttt atatccaacc gtatatgata
    19801 aagcatcaag cataaccatg ttgcgcaatg atgcatcaag tcctaacata acttttaagc
    19861 ttcagaaaaa tattttatat aaaggaaaag ctacagtaaa aaatggtgaa tggtcgttta
    19921 catttattgt acccaaagat atttcttatc agtttgggtt aggcaagcta agcttttatg
    19981 ctgaaaatgg agtagaagat ggtgcaggat attttgatag catagttgtg ggtggttcgt
    20041 ctgcaaatat tatttctgag acagatggac ccggtataaa actatacctt aacgatgagc
    20101 gctttgcttt tggcggcact actaatgaaa aacctacaat tgtatgtttg ctctctgact
    20161 caacaggtat caatacagtt ggtaatggtg ttgggcatga catcacagca ataattgatg
    20221 gaaaagttga tcccgtttac gtactcaatg attattatga agcggactta gacagttatt
    20281 ctagtgggag agttgcttat ccgctatcaa aacttccaga aggaaggcat acgcttaaag
    20341 taaaggcatg ggatattctc aacaactcaa gtgaagctta tacagagttt attgtggctt
    20401 ctgatgcatc attagcacta aagcatgttt taaattatcc caacccgttt acaacgcata
    20461 cgcagttttc atttgaatac aacaagcctt gtgaagattt agaagttcag gttcagattt
    20521 ttaccattgc cggtaaattg gttaaaacca tttctaaaaa gataattacc cccggtacaa
    20581 gaattgatga tttaacatgg gatggtcgtg atgattttgg cgacagaatt ggtcgtggag
    20641 tatatattta cagagtaaaa ctcaatacag cagatgagtc ggcttcagaa acgcaaaaac
    20701 tggtgattct caaatagaaa ccttttttat tttttatcgt atatttgcca gccgcttctt
    20761 aaaaataaca tcataaaata aaaaccactt attgtcgtta ttcaaactta gcttttaaaa
    20821 cttattactg aatttcttta tacatgagat ataaattgtt ttacggtgct ttgtctttag
    20881 cagtactttc agtacaaaat caagtgtctg cacagactga taaagccaac ttacttggtc
    20941 agattaacac tattacaact gctgttccgt ttctgatgat ttctcccgat gcccgtgccg
    21001 gtggtatggg cgatgccggt gttgcatcat ctgcagacgt taacagtatt cattggaatc
    21061 ctgccaaact ggtgtttgca gacaaaaaga tgggtgttgg aatttcttac acaccttggt
    21121 tacgaaaatt agtgccggat ataaatctgg cttacgtaag cttttttacc aagcctaatg
    21181 atgtttctgc aattggagct tcgttacgtt atttctcttt aggtaatatt gattttactt
    21241 ctgaaacagg cgaccctatg ggatcgtaca agcccaatga gtttgctgtt gacgttgcat
    21301 actcaagaaa gttgggtgat gagtttagcg gaggtattgc tttgcgttat atccactcaa
    21361 gtttaacagg tggaattact gttggtggca atgctactaa agcaggaaat tctgtagctg
    21421 ccgatgtttc tttcttctat aaaacaaatc ttgaaatttc aggaaaagat gcagagtttg
    21481 gcgcaggttt ggccattaca aatattggtt caaaaatgtc atataccgat aaaggaacaa
    21541 aggattttat tcctattaat ctgcgcttag gaccttcttt gaaaataaaa ctcaatgact
    21601 ataatgaatt agcattttta gttgatttca ataagctatt agtgccaact ccaccggttt
    21661 attccgattc tactattgca ggtgaagaaa atcctattat tttcgcaggg aaagatccta
    21721 atgttggtgt agcacaagga atttttcaat cattcggtga tgcgccagga ggaggaaaag
    21781 aagaattgcg cgaaattaat attgcaggag gattggaata ttggtacgat aagcaatttg
    21841 cattccgtgc aggatatttc tacgagaaca aaactaaagg aaacagaaag tattttactt
    21901 taggtatagg tttacgctac aacattttcg gacttgattt tgcttatctt attccaacag
    21961 agcagcgcaa tccattagaa aatactttac gttttagctt gtcattcaac tttgattcaa
    22021 tcaaaggaag cgacagtaac gaataaacgg tgaatttcag agttggtttt ggttttgatg
    22081 ttcatcagtt agaacagggc attccttttt ttttaggcgg agttcgaatt gaacattata
    22141 aaggtgccaa aggacatagt gatgccgatg taattataca tgccatttgc gatgccttgc
    22201 ttggagcagc agcacttgat gatattggta aacattttcc ggatacatcc aacgaattta
    22261 aaggaataga ttcctcaatt ttattagcaa aagttgtttc attattaaag gaaaaatcaa
    22321 ttgctattgg taatgttgat gtgagtgtag ttttggagaa acctaaaatt gcaccgtata
    22381 ttcaggcaat gcgggagaaa ttagcttctg taatgcagat tgaaatagac tgtgtttcaa
    22441 ttaaggcaac taccaacgaa aaaatgggtt ttgttggtcg tgaagaaggt gttgctgctt
    22501 atgcagttgc cttgattaac cggcagtagg actaaaattc tgtggctcat tgccacgttc
    22561 tgattcaatt ttatagtaaa agacttctgc aatttttctt gctcttgatt ttgcggtttc
    22621 tgcattgctg ccttcaaaca aaccatctac tgtagcttca aatagtttaa gccagtgttt
    22681 aaaatgcttc atttctattg gcaattgcct gtgggcttca aatgcacttc cattgtataa
    22741 cggttcgttg aagagaatgc tacgccagaa accatacatt tttttcagat gttctggcca
    22801 gcgatcttga atgatgttgt tgaatattgg acctaacaaa tcatcttcac gaatagcact
    22861 gtaaaatgta tcaaccatta atgttatatc atcaggtgta attatgtcat gtctattatc
    22921 catcctcatt ttttattttt tttctatttc aacttctctg atttcacccg atattatttt
    22981 tatttttttg gctgatggtt tttttaagaa tgaattgaag atactttgtg attctaaatg
    23041 attttttaaa tgagtaactc ctttaattag attaatattg aatgttgttg ctttctttcc
    23101 aaaataatat cctatagcac cctgatgtcg gaatacgtaa gcccattctt cgctaccagc
    23161 tatcggatag gcaagtgcag catcatgcca gccatcgcct acctctaaac aggcatcatt
    23221 aaaacgattg ttgtagtttg ctttttcatt gacatcaagt actattccgt tgcattgttt
    23281 gatttgttta taaaaacagt tgtcactgta gctctcaaga ctattgatgc agcgacagag
    23341 attatatttt tcagttatat gcgacagaaa ttcatcggca gcttttcttg aaccgaaata
    23401 tcttaatggt gatgagtttg tttttgaaat atcatgaata aagaattttg ataagccatt
    23461 taaaatatca tttttataaa ccccgaaatt cttttctgat ttgccggcac gattaaagaa
    23521 aggctgatat tctttaatgt attcaatttc gagtaaacat gccaataatt cacatcctgt
    23581 attcatgtag cttatatcat gcaagagtgc tttaattttt ttcgatttgg aattgttgtt
    23641 tcgaaaatgt gaagtaactc tttttttgat attttttgct ttgccgatat acagaatttc
    23701 atgattactg tctgtaaaga aatatatgcc gggtgtttca ggcaacttgc taataagttc
    23761 atgtggtatt tctgtatttt taattggtac actcctatga agcagtgtgt gttcaagacc
    23821 tttttccgaa accaatttct ctaaaatttc actggcagca acagcatcgt tcatggcacg
    23881 atgatgccct tctaaaatta ttccaagatt ttttgtgaga tttcctaaag agtaggagcc
    23941 aaaaccgggg aagatttttc tgctcaaaga acaagtgcat attacttctg cattaaaatc
    24001 aattccgcag cgtttaaatt ctgattttat aaaactgaaa tcgaaagacg cattatgtgc
    24061 tacaaaaatt tttccatcaa gcaactgaaa cagttgctct gcaatttctt taaagcatgg
    24121 tgcattttca actaattcat ta
//
LOCUS       DAOOOL010000025        22754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_172_length_22754_cov_103.941187, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000025 DAOOOL010000000
VERSION     DAOOOL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22754
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_22754_cov_103.941187"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PKU82_03605"
     CDS             <1..1164
                     /locus_tag="PKU82_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011626371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOZ81991.1"
                     /translation="DLLDSLKQSTLKADVKLNITQSLTDAKKAVEDYIAFLKDFKNEN
                     PRSFRLGKDLYAKKFEYEIQSQYTYQQIYDSAMQRKSYLHNEMFKISDKLWSKYFGTK
                     AKPVDSLAMIKMMIDTISVRHVKPDEFQSAIEKQLPELVKFINEKNLIYIDPSKPLKV
                     RREPAYMAGVAGASISSPGPYDKGGNTYYNVGSLSGWSAERAESYLREYNHYILQILN
                     IHEAIPGHYTQLVYSNNAPSIIKAVFGNGAMIEGWAVYGELMMLENGYGDNEPEMWLM
                     YYKWNLRTVCNTILDISVHTKDMTEAQALNLLENEAFQQKAEAQGKWRRVSVTNVQLT
                     SYYTGFKEIYDLREELKKKMGSKFNLKAFHEKFLSYGSAPVKMIREEMLSEIK"
     gene            1166..1507
                     /locus_tag="PKU82_03610"
     CDS             1166..1507
                     /locus_tag="PKU82_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81992.1"
                     /translation="MIRFERFLTLIKRQFYIYVGGLFLLLSIRLFFLFRLFSIAEIKL
                     NVVDILHALAVGFRFDTMVLMYGMALPVLLSILSLIVGTAKSKDMVMRFNRVYAIVML
                     MLFVIVGIVVR"
     gene            1519..2925
                     /locus_tag="PKU82_03615"
     CDS             1519..2925
                     /locus_tag="PKU82_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOZ81993.1"
                     /translation="MQIDDSTVCNNSTINLIPINGVFAIKEAFLMRSKQMSIEGNITF
                     LKKLGYGSEVKAIEDYYGHAPEKDLNKSYFTVTPNKPSGFKHPNVVFFLMESMSNNNL
                     FYHSATLNLYGALEKHLSTDYFLRNFLPSGNGTINSLEGIMVNTPVTSLAQSEHNSVS
                     YSSSVALPFRNSGYETTFITGGKFGWRNINEFIPHQYFQHVESKDNVLKEIAGSTECE
                     WGVYDEYLFDYVFKKLEKATKPQMIFVLTTTNHTPFHLPETYRPYPIKLTNEINSKLL
                     TDENLAVKNLTNLQYSNDCLGKFMDKVKHSNLSQNTIVAATGDHNNLMLFDFNESQMY
                     YQRSVPLYMYVPETIHPHVAINKWGSHKDIFPTLINLALPQSTYFNAGDNLFDTLQSR
                     NNIFATDWMSMEAMNEFGAVKYGESVKYFDWDKNKMLKETATPSADLQKLLQRARAHY
                     AAMAYFIRSETLKTKQGK"
     gene            complement(2929..3171)
                     /locus_tag="PKU82_03620"
     CDS             complement(2929..3171)
                     /locus_tag="PKU82_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ81994.1"
                     /translation="MEDFIGFIGVFILLVAYFLHLFRIIKPDKLFYLILNFIGASLAC
                     LASVLIHYMPFIILEAAWALVSLISIIKLFLLKPTN"
     gene            3250..4389
                     /locus_tag="PKU82_03625"
     CDS             3250..4389
                     /locus_tag="PKU82_03625"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOZ81995.1"
                     /translation="MKFATKALHAGVEPDPTTGAIMTPIYQTSTYVQASPRQHKGYAY
                     ARGKNPTRSQLEKNIAALENAKYGLCFSSGMGATDAVMKLLKPGDEVISGDDLYGGTY
                     RMFTKVFANYGIKFHFINLTDANNISKYINSNTKLIWVETPTNPTMQIVDIKACSAIA
                     KKNNCILAVDNTFASPYLQNPMDLGADIVMHSVTKYLGGHSDVVMGALCMSDDSLYER
                     LAFIHNSCGATPGPMDSFLVLRGIKTLHLRMERHCFNGKQIAAFLRAHPKIEKVYWPG
                     FEDHPNHHIAKMQMRDFGGMISFDLKSHSQEEAFKLASSVKVFSLAESLGGVESLVNH
                     PASMTHASVPVAERVRVGVTDSLLRLSVGVEDIDDLIADLKQAIG"
     gene            4474..6567
                     /locus_tag="PKU82_03630"
     CDS             4474..6567
                     /locus_tag="PKU82_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ81996.1"
                     /translation="MLKKSTNFLLLMMAMIATITVNAQNTHWCATDEVTNAALQNSAA
                     ARAAQNQLEAFTQQYVQNFQPSYFRSTGTAVYVIPVVFHVIHNWGPENISDAQVYDAI
                     RVLNRDYNGQRTDTANIIPDFKALNADVQIEFRLAHKDPNGNCTNGIDRQQSLLTYDA
                     GDQSKLNPWPYNKYLNIWTISNFGSSHAQAAAYAYKPGTAPPGKDGIIALHNYVGSIG
                     TGSSTGQYTLTHEIGHWLNLNHTWGGTNQPGVACGDDNVTDTPVTKGYTTCPSASNAD
                     VCNPGVIENYQNYMDYSYCYYMFTLGQKARMWAALNSGAGLRNQVHTATNLTNTGTDG
                     GTYVCAPTASFSPVQKVICEGGTVSFSDASLNTDTVGTTYLWTFNGGTPSTSTDKNPT
                     GIIYNTAGIYDVTLTVTTSAGFDTYTGIESVVVRPQQVSNFVPYSEGFENVLFPSVDW
                     IVDNENGNAWELNSTAAHSGFNSVRINNFSGNVAGTKDVFITPGFNLTNTTQTQFTFW
                     LAYALKNSSGTDQLKVYASNNCGQLWNLRTTKTGTQLATVSSTVTSDFVPNAADWRQE
                     TVSLTSGQYSTKPNVSLKFEYTHDKGNNIYIDDINLTGVVGISDYAVSASDVTIVPNP
                     ASEKSSVNFELLQPEKVLITVTDMLGKEVHRISENRLDAGEYQYEVNTNWAKGVYTVR
                     IAAGSQIISKKLVVE"
     gene            6631..7041
                     /locus_tag="PKU82_03635"
     CDS             6631..7041
                     /locus_tag="PKU82_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOZ81997.1"
                     /translation="MSESKFSIEMNYVVYAKPEAVFNALTNPDVMKKWIEGSFVFELK
                     KGGNVELFDGWVKGEVITFINNESFSYTWKPNTWDKKTKPSLVEIELKPDAAGTRVYI
                     HHSLFPSNDDTDKHRHGWINFVLDPLNDYFIGLM"
     gene            complement(7048..8529)
                     /locus_tag="PKU82_03640"
     CDS             complement(7048..8529)
                     /locus_tag="PKU82_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOZ81998.1"
                     /translation="MKIKNITTLGDLKKNGYKSRSIKEEMRENLIRKLKSGEKLFEEI
                     QGYEDTVIPQLQTAVLSKHNIILLGLRGQAKTKIARLMTQLLDEYMPIVSGSELNDDP
                     LNPLSRYAINIIEDEGDTTPITWIHRNDRYTEKLATPDVSVADLIGDIDPIKAAAQKL
                     NYSDERVIHYGLVARSNRNIFVINELPDLQPRIQVALFNILQEGDIQIRGFKLRLPLD
                     IQFVFTANPEDYTNRGSIITPLKDRIGSQILTHYPKNIEIAGAITLQESKIEKSQSEN
                     IFIAPLLHQLIEQTAMEARQSELVDQKSGVSARLTISALENLYSTCEQRMLLNHENKS
                     NGRISDLQGIIPSITGRVELVYEGEAEGIQSVALTLMDKAIRKVFAETFTDPEKLKKS
                     KNTNPYSEIINWFEQGNTLSLLHLDTTAQYIKKLDEVHGLKKTVKQFLKNINENEVHL
                     WMEFLLYGLAAFSQISKTALERNTEFKDLMRTVFNFKSMDDEV"
     gene            complement(8533..9627)
                     /locus_tag="PKU82_03645"
     CDS             complement(8533..9627)
                     /locus_tag="PKU82_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ81999.1"
                     /translation="MHGTRFTAFIPEKENTPFDKLLSIFMELLPYTAGDVSEALSWMT
                     ELDKQYKLTDKSYGMGDFIKDLKEKGYIKDNPDNDKFSLSSKSERTIRKRALEEIFGK
                     LKKSNTGNHKTSFTGQGDEWTSDLRPFQFGDNIDSINFTQSLQNAQINHGPDEFNLTE
                     NDLEIQEKEFKTQHSTVLMIDISHSMILYGEDRITPARKVAMALAELISIKYPKDTLD
                     IIAFGDDAFPIAIKDLPYLQVGPYHTNTTAGLQLAMELLRRKKTQNKQIFMITDGKPT
                     CIKSGASYYKNSFGLDQKIINSVFSLASQCRKQKIPITTFMIATDPYLQQFVRQMTAI
                     NKGRAFYSSLNGLGEFVFEDYVKNRKKRVL"
     gene            complement(9822..10193)
                     /locus_tag="PKU82_03650"
     CDS             complement(9822..10193)
                     /locus_tag="PKU82_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOZ82000.1"
                     /translation="MATKKAKKAPAKKAAPKKKAAPKKKAAAPKKKAAAKAKTKRTPN
                     AAFMKAMTIGEALHPVVGSKPLPRTEVVKKLWEYIKKNKLQDPKEKRNINADDNLKKV
                     FGGKKTVSMFEMTKLVSKHLS"
     gene            10634..12064
                     /locus_tag="PKU82_03655"
     CDS             10634..12064
                     /locus_tag="PKU82_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82001.1"
                     /translation="MKKAIFITLTCVVALSTVRGQDMWGIANSNYAGNFAIDINPSSM
                     AVSPIKYELNILSGNMTFYNNYLYLPKGKMSVGKFFKADYFNYDWQDQYTGKDKNAFF
                     QTGIKGPSFFLRSGQTAFGLHTGLKAMGSVRNVDFEMAKLFYENSTYTPLQHTELEAG
                     NNKLAALLYGEIGASFAQQIEAGPGNRIAIGATFNYLMGFDGFYAQNDHMQYRFDDES
                     MLHVTDLQAEYGYAVPQDGKSDYLKVRGKGFSTTWGVQYIHGLNPEAYNVYASRLKFK
                     KYKYKIGMSLVDAGSILFAKDTRTVKFDNNNIDWSGFDTTGVESISQLGELIRSKVFD
                     DSTSGYVATKYRAKLPAAISIQFDYCLTHFLYLNTSIIQPVTSKSTAIRRPAQLSFTP
                     RLETWRYEISFPLTWYEYDQVRLGTCLRLGQVVIGSDYLTSMLGLSKFNGFDFFVGIK
                     HSSYAMDKLSKRKKEKVKDTYTGGDI"
     gene            12144..13142
                     /locus_tag="PKU82_03660"
     CDS             12144..13142
                     /locus_tag="PKU82_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HOZ82002.1"
                     /translation="MNIKQTEQFLKKVKGMHTLVIGDVMTDAYLWGRVDRISPEAPVP
                     VVAVQKRESRPGGAANVAVNLHSLGAIPFICSVVGKDNAGKEFREMITKSGMPADGIL
                     ASSKRTTTIKTRVIGNNYQLLRVDAEVAEPLSQADGEMLIKKAIEIIRKKKPAVIVFE
                     DYDKGVLNKTVIEAIVAEAQKQNIPTTVDPKKNNFFHYRNVTLFKPNLKELREGLKTD
                     LEKPTATALTKLASDFATKQKIKSLLITLSEHGVYYYSKNEKGIIPAHFRNISDVSGA
                     GDTVISVASLAIAAGFSCHEMAAISNLAGGLVCEKVGVVPIDGNQLLNEVKRLSGK"
     gene            13144..13863
                     /gene="ubiE"
                     /locus_tag="PKU82_03665"
     CDS             13144..13863
                     /gene="ubiE"
                     /locus_tag="PKU82_03665"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOZ82003.1"
                     /translation="MQVTPYNTGESKKEQVQHMFDAVAHRYDFLNHFLSLGIDKIWRK
                     KTIKEIGKLKPRTILDIATGTADLAIAATSLNPERIIGVDISEKMLAAGRVKIEAKNL
                     NNIISLQQGDSEQLQFENQTFDAVMVAFGVRNFQNLEKGLMEMKRVLNSNGKVFILEF
                     SKPTMFPIKQFYGIYSRYLLPLFGKLISGDASAYTYLPESVKHFPSGNDFALVLKQCG
                     FTNIEIKPMTFGIVTLYIAAA"
     gene            13860..14570
                     /locus_tag="PKU82_03670"
     CDS             13860..14570
                     /locus_tag="PKU82_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ82004.1"
                     /translation="MKQRVIVFSFLVLLFNADSLFAQKQKILNLPKYDRQRFHFGFIL
                     GINKTDFVIDRVASFSLIDSLYTVESKGTSGFNLQIVTNMRMGEHFDLRFLPGLAFAS
                     RNLIYTFEKGRPLSNVVTKKVESTFVELPLDVKFKSARLNNFRAYVLGGVKYSIDMVS
                     QAKVKAKDKEYVKLQRNDYGYEIGVGCDFYLPMFKFSPEIKMYHGLRNLLVKDDLVYS
                     KSLDGLFSKMFTLSLTFE"
     gene            14586..16142
                     /locus_tag="PKU82_03675"
     CDS             14586..16142
                     /locus_tag="PKU82_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOZ82005.1"
                     /translation="MQTFELVVLPEEAASESVIQHIVAKKFGGNRLFRIIKRSIDARS
                     KTVKIRLQLQWTDEQNQETLVENRKWRQVANSEPVIIAGCGPAGLFAAIRLIECGFKP
                     IIIERGKDVRNRRRDLAAINKQRQINPESNYCFGEGGAGTYSDGKLYTRSNKRGNIAS
                     VLNTFIEHGADGDIAVDAHPHIGTNKLPAIISAMRNRIVECGGEVLFDTKLVNIDFDT
                     SGVNGVEVQQGLAQFHIKSKALILATGHSARDVFYMLNNQKILIEAKPIAIGVRAEHQ
                     QQVIDSVQYHCSKRSQFLPPASYALKHQHDGRGIYSFCMCPGGIIAPAATDIGEVVVN
                     GWSPSRRNNVFANSGIVVSITEEDVLPFKNAKELRFLELQKSIEEKAFVSGGSNLAAP
                     AQRIVDFVNQRVSSNLPTCSYIPGINSVNLKEIFPASVTNSLAAAFVAFGKKMKGYYT
                     NEAVLVGTESRTSSPVRIPRDVETAMHVQVKGLFPCGEGAGYAGGIVSAAMDGEFCAE
                     KCAAYLKNNN"
     gene            16175..17260
                     /locus_tag="PKU82_03680"
     CDS             16175..17260
                     /locus_tag="PKU82_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOZ82006.1"
                     /translation="MEINQPPYYIRITFKILLAILIFYVLHAAQDVLIPLTVAVLFTF
                     LLMPVSNKLIKWKFPPAIAILCSILLAFVAVGGLLYFFYSQIVSFAEDLPLLQAKLLE
                     KVEFLQRFVNETFRITKEQQNVLVKSKLQENIADSGLLLLNLFSSTGTFIANFALVTV
                     YVFFLTLYKKKFKQFIEAIVSDGKHNSYFQIVKKVAGVSQNYLKGIFLDVLILSILNS
                     TGFLLLGIEHAVLFGVLASVLNIIPYIGVLIGSVLPVLMALLTKDEIGYAIGAAGVCV
                     TVQFIDNNFITPYVVGSSVSINPLTATIALIISASVWGLAGMVLCLPLTGMLKVVCDH
                     VEGLKPIGFLIGEEVDYRKNKFKLGRK"
     gene            17340..17993
                     /gene="scpB"
                     /locus_tag="PKU82_03685"
     CDS             17340..17993
                     /gene="scpB"
                     /locus_tag="PKU82_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOZ82007.1"
                     /translation="MENLKQHIEALIFTSEQSITANEIAAALKASFGWELSNENIRQS
                     ISELINKYSADDYSFELVEIAGGFRFLSKKEYYGVVNTMLQINSKKRLTTAALETLAI
                     VAYKQPVSKSEIENIRGVNCDYSIQKLLEKDLITIQGKGDGPGKPILYGISRTFMDHF
                     GLKSAKDLPKLKDVILDENEIGTPADMQYAENDNAEGVSITLGTQEGNTDKTDDVAE"
     gene            18024..18857
                     /locus_tag="PKU82_03690"
     CDS             18024..18857
                     /locus_tag="PKU82_03690"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOZ82008.1"
                     /translation="MKISVLGLQFDSALKNVFSMLSIALICLMQPSISEGQEKLRKVE
                     NQAFTYGEVLEYRVHYGIIDAGEAKLAVMPDKKTIGPREVYHVVGTGQTKGAFDWFFK
                     VRDRYESFIDAQAIIPWLFVRRVYEGGYKINQDVTFNQVKNLAISKKATIATPQNVQD
                     LISAFYYARTIDFSNAQPGALYDVDAYLDDAIIPVKFKYVGKENISTKFGTFRCLKFH
                     PQLLEGRVFKNKEDMTLWVSDDENKIPIRAEAKILVGSVKMDIKSFSGLANETLSLLK
                     K"
     gene            complement(19228..20046)
                     /locus_tag="PKU82_03695"
     CDS             complement(19228..20046)
                     /locus_tag="PKU82_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82009.1"
                     /translation="MFAVATAFNGSAQFLKTDSVGNALWNVGFTDSTFYNLFFDQITP
                     DSGNTFVTAGNFDIFNPSINAAARIDSAGNLLSMHQYNNYSNCHSYSHYFNPQTGLAM
                     FGYGGGFNNFGFYFCKTDSVLTTPCMSTPSTFTTYSYSVTDSATSILSQPVSLVSIDV
                     LAQMSVNSISFTDSSLCPLVSSVPSFQNQNTLQVLNADVSLYPNPFSSQLTFSLASNE
                     KTTASLYNFVGQRVLQQTFSNSTTINTEQLPEGIYYYALHNYKGVFKTGKVIKQ"
     gene            complement(20070..20801)
                     /locus_tag="PKU82_03700"
     CDS             complement(20070..20801)
                     /locus_tag="PKU82_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82010.1"
                     /translation="MKKLLQFSFILATYLSSGQTAFEKMFKPSGVSDIFLTYYDVIKS
                     PDDSGYYTLSTFGGPDSGAILSLVGNDGILKWSKSYQRVTAIFKTFGLSVLPLSDSTL
                     MLNLYDDGRTIQLKTSYSGTMLGAKKLNYISSTVYFQEEKNNAIYGVGRVSANGVNGG
                     LFCKMDLDGNIIWSRMMNGGTSRRIELRALTLTSDGGFLLAGTRQIYGTILPASTILI
                     KTDSIGNITWQRQLNYVNKILFLLP"
     gene            complement(21354..>22754)
                     /locus_tag="PKU82_03705"
     CDS             complement(21354..>22754)
                     /locus_tag="PKU82_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82011.1"
                     /translation="GVFLSTDTGNTWNALNNGLTNLHVSAIAASGANVFAGTAGGGVF
                     FSSDTGNTWTSANNGLTSSTIYSFLINNSDIFVGTSADGLFLSTNNGASWNAQNTGLA
                     SSFVHSLAVSGSNLFAGSNYKGMYRSTNNGNSWSQINNGLTSTFINSILATTNDLFVG
                     TVNGLFMSADTGNTWVQSDSGITNKFIIAVAKLGSHLIAASYLNEVYLSVNNGSTWNL
                     INNGLPTGGSSSLVISGSNIFLGTYQNGVFLSTDTGNTWNAVNNGLTNLHVSSLAISG
                     TNIFAGTDSNGVFFSSNNGNSWNAVNNGLPTTSIITCLAINGTNILAATMQDGVYFSN
                     NNGTTWSAVNNGLTNKIVTDLTISGINFFAATQGNGVFLNSNLLTDITENTNYYSSIN
                     IYPNPALSQVVIEFNHVIDNGKIQMFNTFGNKIIEENIISESKKVINLNNISNGIYFM
                     KVFDGKNSNCKKLIVE"
BASE COUNT         7324 a         3722 c         4462 g         7246 t
ORIGIN      
        1 gatttattag actcactgaa acaatctaca ctaaaggccg atgttaagct aaacattact
       61 caatcactga ctgatgcaaa aaaagctgtt gaagattata ttgcattttt aaaagacttt
      121 aagaatgaaa acccaagaag ttttaggttg ggaaaagatt tgtatgcaaa aaagtttgaa
      181 tacgaaatac agagtcaata tacgtatcag cagatttatg attctgcaat gcaacgaaaa
      241 tcttatttgc ataatgagat gtttaaaatc tcagacaagt tatggtcaaa atattttgga
      301 acaaaggcta aacctgtaga tagtttagca atgattaaaa tgatgataga taccatttct
      361 gtacgtcatg taaagcctga tgaatttcaa tcagcaatag aaaaacaact tcctgaattg
      421 gttaaattta tcaatgaaaa aaatctgatt tatattgatc cttcaaaacc attaaaagta
      481 aggagagagc ctgcttatat ggcaggagta gcaggtgcat ctatcagttc acctgggcct
      541 tatgataaag gaggtaatac atactataat gtgggttcac tctcaggttg gtctgctgaa
      601 cgtgcagaaa gttatttacg agagtataat cattacattt tgcagatttt gaatattcac
      661 gaagcaatac cgggacacta tacgcaattg gtctatagca ataatgcacc aagtattatt
      721 aaggcagtat ttggaaatgg cgccatgatt gaaggatggg cagtttatgg ggagttgatg
      781 atgcttgaaa atggctatgg tgacaatgaa cctgaaatgt ggctgatgta ttacaaatgg
      841 aatctgagaa cagtttgtaa taccatttta gatatttctg ttcacactaa ggatatgaca
      901 gaggcacagg cattaaattt attggagaat gaagcatttc agcaaaaggc agaagcacaa
      961 ggtaagtgga ggagagtaag tgtaactaat gttcagctta caagttatta taccggtttc
     1021 aaggaaattt atgacttacg cgaagagtta aaaaagaaaa tgggaagcaa gtttaatctg
     1081 aaagcttttc atgagaaatt tttgagttac ggtagtgcac ctgttaaaat gattcgtgag
     1141 gaaatgctct ctgagataaa ataacatgat tcgtttcgag cgctttttaa ctttaattaa
     1201 acgacagttt tatatttatg taggcggact ctttctgctt ctgtcaattc gtttattttt
     1261 tctttttcga ttattcagca ttgctgaaat taagttaaat gttgttgata tattacatgc
     1321 attggctgta ggtttcagat ttgacacaat ggtgctgatg tatggaatgg cattacctgt
     1381 tttgttaagt attttatctc tgattgttgg aactgcaaag agcaaagata tggtgatgcg
     1441 ttttaacaga gtttatgcaa tagtcatgtt gatgcttttt gtaattgttg gaatcgtggt
     1501 tcgataggca catttccatt gcagattgat gactctacag tttgtaataa cagcactata
     1561 aatctgattc cgataaatgg tgtgtttgca ataaaggaag catttctgat gcgtagcaag
     1621 cagatgagta ttgaaggcaa cataaccttc ttgaaaaaat tgggttatgg tagtgaagta
     1681 aaagctattg aagattatta tggtcatgca ccggaaaaag atttaaacaa atcatacttt
     1741 acagtaacac ctaataagcc ttctggattt aagcatccta atgttgtttt ttttctgatg
     1801 gaaagcatga gtaataataa tttgttttat cattcagcta cacttaatct atatggtgca
     1861 ttggaaaaac atctttcaac agattatttt ttaagaaatt ttttaccatc aggtaatggt
     1921 actatcaaca gccttgaagg cataatggtc aatacaccgg tgacatcatt ggcacagtca
     1981 gaacataatt cggtttctta ttccagttct gttgcattgc catttagaaa ttcaggttat
     2041 gaaacaacat ttatcacagg cggtaagttt ggctggcgaa acatcaatga gtttattcct
     2101 catcagtatt ttcagcatgt ggaaagtaaa gataatgtgc tgaaagaaat tgcaggctca
     2161 acagagtgcg aatggggcgt ttatgatgag tacttatttg actatgtgtt caaaaagttg
     2221 gaaaaggcca ctaagccaca aatgattttt gtactcacca caacaaatca tacacctttt
     2281 catttgccgg aaacatatag accatatcct ataaaactga ctaatgaaat aaactcaaaa
     2341 ttacttaccg atgagaacct tgctgttaag aatcttacta atctgcaata ttccaatgat
     2401 tgcttaggta aatttatgga taaggttaag cactccaatc tcagtcaaaa tacaatcgtt
     2461 gcagctacag gcgaccataa taatttaatg ctatttgatt ttaatgaaag tcagatgtac
     2521 taccagcgaa gtgttccact gtatatgtat gtgccggaaa caattcatcc acatgtggca
     2581 atcaacaaat ggggttcaca caaggatatt tttccgactt taataaatct tgcactgcca
     2641 caatcaactt attttaatgc cggagataat ttgtttgaca cattgcaaag tcgcaataat
     2701 atttttgcaa cagactggat gagcatggaa gccatgaatg aatttggtgc tgtcaagtat
     2761 ggtgagtctg taaaatattt tgattgggat aagaataaaa tgctgaaaga aacagcaaca
     2821 ccatctgccg atttgcaaaa attacttcaa cgcgcaagag cacactatgc tgcaatggct
     2881 tattttatca gaagtgaaac tttaaaaact aaacaaggaa agtagtgttt aatttgttgg
     2941 tttcaataga aaaagtttga taatagaaat aagtgaaacc aatgcccatg cagcttcaag
     3001 aataataaag ggcatataat gaatcagaac agaagccaag catgcaagag aagcgccaat
     3061 aaaattcaga ataagataaa agagcttatc cggtttaata attcggaaaa gatgaaggaa
     3121 atatgccact aaaaggataa aaacaccaat aaatccaata aaatcttcca taaaatgttt
     3181 aatttaacca gaaccaaagg tatggcaatg gttgtataat ttatcttcgt acgtttaaat
     3241 tttgtaacaa tgaaattcgc aactaaagca cttcatgcag gcgttgagcc agatccaact
     3301 accggtgcta tcatgacacc tatttaccag acttcaacct atgtacaggc atcaccccga
     3361 caacataaag gttatgccta tgctagagga aaaaacccta cccgttcgca gttagagaaa
     3421 aatattgctg cattagaaaa tgcaaaatac ggattgtgtt tcagcagcgg aatgggagct
     3481 accgatgcag tgatgaagtt gttaaagccc ggtgatgaag taatttccgg tgatgatttg
     3541 tatggtggaa catatcgtat gtttaccaag gtttttgcaa actatggtat taagtttcat
     3601 tttataaatc taactgatgc taacaatatc agtaagtata tcaattcaaa cacaaaactc
     3661 atttgggttg aaacaccaac caatccaacc atgcagattg ttgacataaa agcttgcagc
     3721 gcaattgcaa aaaagaacaa ttgtattttg gctgttgaca acacatttgc atcaccctat
     3781 ttgcagaatc caatggattt aggtgcagac attgtaatgc attcagttac taaatatctg
     3841 ggcggacaca gtgatgttgt tatgggtgca ttgtgtatga gtgatgattc attatatgaa
     3901 agacttgcct ttattcataa ctcatgtggt gccactccgg gacctatgga cagttttctt
     3961 gtattaagag gaataaaaac tttacatctt cgcatggagc gtcattgttt taatggaaaa
     4021 caaatagcag catttcttcg tgctcatcca aaaatcgaaa aagtttattg gccgggcttt
     4081 gaagatcatc caaaccatca cattgcaaaa atgcagatgc gcgattttgg tggtatgatt
     4141 tcttttgact taaaaagtca ctctcaggaa gaggctttta agttagcgtc atcggtaaaa
     4201 gtgttttcat tggctgaatc attgggtgga gtagagtctt tggtaaacca tcctgcatca
     4261 atgacacatg ccagtgttcc ggttgcggaa agagtaagag ttggtgtaac cgactcatta
     4321 ttgagattaa gtgtgggtgt tgaagatata gacgatttaa ttgcagattt gaaacaagca
     4381 atagggtagt tccttcacaa aaaatttaca ttgaacatat gctcaaaaca cacgatttta
     4441 ttacttttga ttcattataa ataaattaaa acaatgctaa aaaaatctac taatttttta
     4501 ttactgatga tggccatgat tgcaaccatt acagtcaatg cacagaacac acattggtgt
     4561 gctactgatg aagttactaa tgctgcactg cagaatagtg ctgctgcccg tgctgcacaa
     4621 aatcagttag aagcgtttac gcaacagtac gtacaaaact ttcaaccaag ctatttcaga
     4681 agtactggca ctgctgtgta tgttatccct gtggtatttc atgtcattca taattggggt
     4741 cctgagaata tttcagacgc gcaggtttat gatgcaatca gagttttgaa ccgcgattat
     4801 aacggacagc gaacagatac agcaaacatc atccctgatt ttaaagcatt gaatgccgat
     4861 gttcagattg aattccgtct tgcacataaa gatcctaatg gaaattgtac taatggtatt
     4921 gacagacaac aatcgttgtt aacttatgat gccggtgatc aatcaaagtt gaatccatgg
     4981 ccttacaata aatatttaaa tatctggact atttctaact tcggttcatc gcatgcacaa
     5041 gcggcagctt atgcatacaa acccggtaca gcgcctcccg gtaaagatgg aatcattgca
     5101 ttgcataact acgttggtag catagggact ggtagttcaa caggtcaata tacacttacg
     5161 catgaaattg gtcattggtt aaatctgaat catacatggg gtggaaccaa tcaacccggt
     5221 gtagcttgcg gtgatgataa cgtaacagat actccggtaa ctaaaggtta tactacatgt
     5281 ccatccgctt caaatgcaga tgtgtgtaac ccgggtgtta tagaaaacta tcaaaactac
     5341 atggactata gctattgcta ttacatgttt actttaggac agaaagcgcg tatgtgggct
     5401 gcattaaatt caggtgcagg cttgcgtaat caggttcata ctgcaaccaa tttaactaat
     5461 acgggaaccg atggaggtac gtatgtttgt gctcctacag caagtttttc acccgtacaa
     5521 aaagtaattt gcgaaggcgg aactgttagc tttagtgatg caagtttaaa tacagataca
     5581 gtgggcacaa cttatctgtg gacttttaac ggtggtactc catcaacctc aacagataaa
     5641 aatccaacag gtatcattta taatactgct ggtatttacg atgtaacatt aactgttact
     5701 acttctgcag gttttgatac ttatacaggt attgaaagcg ttgttgtaag accgcagcag
     5761 gtttctaact ttgttccata ttcagagggt tttgaaaatg tgttattccc ttctgtagat
     5821 tggattgttg ataatgaaaa tggcaatgca tgggaactga attctaccgc tgcacattca
     5881 ggatttaatt ctgttagaat aaataatttc agtggcaatg ttgcgggtac caaggatgta
     5941 tttattacac caggttttaa tttaacaaac actacacaaa cacaatttac tttttggcta
     6001 gcttatgcac taaagaacag ctccggtaca gatcagttaa aagtttatgc ttctaacaac
     6061 tgcggtcagt tgtggaacct gcgtacaaca aaaacaggaa cgcaattggc aacagtaagt
     6121 tcaacagtaa cttcagattt tgttcctaat gcagcagact ggcgtcagga aacagtaagt
     6181 cttacttcag gacagtattc aactaaacca aacgtttctt taaagtttga atatactcac
     6241 gataagggta ataatattta tattgatgac attaacctta caggtgttgt tggtatcagc
     6301 gactatgctg tttcggcaag tgatgtaact attgttccta atcctgcttc agagaagtca
     6361 agtgtaaact ttgaattgct gcaacctgaa aaagtactga ttacagtaac tgatatgttg
     6421 ggtaaagaag ttcatcgaat ttcagaaaac cgtttagatg ctggagaata tcaatatgaa
     6481 gtaaacacaa attgggctaa gggagtatat acagttcgta ttgctgccgg aagtcagatt
     6541 atcagtaaaa aattagtagt agaataaatt aagtaaatat taattaagcc tgaagctgaa
     6601 tttggtttca ggcttttctt tttgatggtt atgagtgaaa gtaaattttc aattgaaatg
     6661 aattatgtgg tttatgcaaa accggaagcg gtgtttaatg cattgacaaa tcctgatgtg
     6721 atgaaaaaat ggatagaagg aagttttgtt tttgaattga aaaaaggagg taatgttgaa
     6781 ttgtttgacg gttgggttaa gggcgaagtt attacgttta ttaacaatga atcattcagc
     6841 tatacctgga aacccaatac atgggataaa aagaccaaac caagcttagt ggaaattgag
     6901 ttgaagcccg atgctgccgg tacaagagtg tatattcatc acagtttgtt tccaagtaat
     6961 gacgatacag ataaacacag acatggatgg atcaattttg tacttgaccc gttgaatgat
     7021 tatttcatcg gattgatgta ataaagttta aacttcatca tccatagatt taaaattgaa
     7081 tactgttcgc attaaatctt taaactctgt gtttctttcc aaagcagttt tgctgatctg
     7141 agaaaaggca gcaagtccat acaatagaaa ttccatccat agatgaactt cgttttcatt
     7201 tatgtttttc agaaattgtt tcactgtttt tttaagtcca tgaacttcat cgagcttttt
     7261 aatgtattgt gctgttgtat cgagatgtaa aagactcaat gtatttcctt gttcaaacca
     7321 atttataatt tctgaatatg gattggtatt ttttgatttt tttaattttt cgggatcagt
     7381 aaatgtttct gcaaaaactt ttctgatagc cttatccatc aaagttaatg ctactgattg
     7441 aatgccttcg gcttcacctt catagaccaa ttccacacgc ccggtgatag atggtattat
     7501 gccttgcaaa tcgcttatgc gtccattgct tttgttctca tgatttaata acatcctttg
     7561 ttcgcatgtg ctgtacaaat tttcaagtgc agaaatggtc agtcgtgcac tcacaccact
     7621 tttctgatca accaactcgc tttggcgggc ttccatggca gtttgctcaa tgagttgatg
     7681 tagtaaagga gcaataaaaa tattttcaga ttgagatttt tcaatttttg attcctgcaa
     7741 ggtaatagct cctgctattt caatattctt tggatagtgt gtaagtatct ggctgcctat
     7801 acggtctttt aagggtgtga tgattgaacc tctgttggta taatcttcgg gattggcagt
     7861 aaacacaaat tgaatgtcta aaggcagacg cagtttgaaa ccgcgaatct gtatatcgcc
     7921 ttcctgcaga atgttgaaca atgcaacttg tattcggggt tgtaaatccg gaagttcatt
     7981 gataacaaaa atgtttctgt tgcttcgggc aactaaacca taatgaataa cgcgctcgtc
     8041 agaatagttt aacttttgtg cagcagcttt aataggatca atgtcaccaa tcaggtcagc
     8101 tacagaaaca tctggagtag ctaatttttc agtgtaacgg tcgtttctat gtatccatgt
     8161 tatgggtgtg gtgtcgccct catcttcaat aatattaata gcatatcgcg aaagcggatt
     8221 cagaggatcg tcatttaatt cagaaccact tactataggc atgtattcat ccaacagttg
     8281 tgtcatcagt cgtgcaattt ttgtctttgc ctgaccgcgt agtcctaaaa gaatgatgtt
     8341 atgtttagat aagacagcag tttgcagttg tggaattact gtatcttcat agccttgtat
     8401 ttcttcaaat aatttttccc ctgatttgag tttacgaatc aggttttctc gcatttcttc
     8461 tttaatagac ctggacttgt atccgttttt tttcaaatcg ccaagtgttg ttatattttt
     8521 aatcttcatt ggttatagaa ctcttttttt acggtttttt acataatctt caaatacaaa
     8581 ttcgcccaat ccattcaggc tgctgtagaa tgcacggcct ttatttattg cagtcatttg
     8641 tcttacaaat tgctgcaaat aagggtcggt agctatcata aaagtagtga taggaatttt
     8701 ttgtttgcgg cattgtgatg ccaacgagaa aacactgtta attatctttt ggtcaaggcc
     8761 aaaactattt ttatagtaag atgcacccga ttttatacaa gtcggtttgc cgtctgtaat
     8821 cataaaaatc tgtttgttct gtgttttttt tctgcgtaat aattccatgg caagttgtag
     8881 tcctgctgta gtattggtat gataaggacc aacctgcaaa taaggcaaat cttttatggc
     8941 aataggaaaa gcatcatcac caaatgcaat aatgtctaaa gtatctttag gatattttat
     9001 actgataagt tctgccaatg ccatagctac ctttcgtgcg ggtgttatcc tatcttctcc
     9061 ataaagtatc atggagtgcg atatgtcaat catcaaaact gttgaatgct gggttttaaa
     9121 ttctttttcc tgaatttcaa gatcattttc tgttaagttg aattcatctg gtccatgatt
     9181 aatttgtgca ttctgcagtg attgagtaaa gttgatgctg tcaatattat cgccaaattg
     9241 aaagggacgt aaatcagaag tccattcatc tccctgaccg gtaaacgatg tcttatgatt
     9301 tcctgtattt gattttttaa gctttccgaa aatttcttcc aatgcacgct tacgaatagt
     9361 cctttcagat tttgaactca gactgaattt atcgttgtcg ggattatcct tgatataacc
     9421 tttttctttc agatctttaa taaaatcacc cataccataa cttttgtcag ttagtttata
     9481 ttgtttatca agttctgtca tccatgataa ggcttctgaa acatcaccgg cagtataggg
     9541 taacaactcc atgaaaatac tgagtagctt atcaaatgga gtgttttctt tttcaggaat
     9601 aaaggctgta aaacgtgtgc cgtgcatgat gtgtgttatg ggagtttaaa aatacaacaa
     9661 accaattaac aactggatac tcggaatgtt tacggtataa atttatgaga attggacaag
     9721 acattgccat tgtgtgatta gtttttcatg gtacgacatg gctaaatgaa aaaaggggag
     9781 gctttttaaa gcctcccctt tttgttcttt cttacagaat attaagaaag gtgtttgcta
     9841 actagtttag tcatttcaaa catagaaact gtttttttgc caccaaaaac ttttttcaag
     9901 ttgtcatcag cattgatgtt acgtttctct tttggatcct gtaatttgtt tttcttaata
     9961 tattcccaaa gttttttaac aacttcagta cgtggaagtg gcttgcttcc tacaactgga
    10021 tgaagagctt cgccaattgt catagctttc ataaatgcag catttggagt acgctttgtt
    10081 ttagcttttg ctgctgcttt ctttttagga gctgctgctt ttttctttgg agcagctttc
    10141 ttttttgggg ctgctttttt agcaggtgcc tttttagctt ttttagttgc cattttgaaa
    10201 tttgtttttt ttaatttgtt gagcaaatga aatacataat ttttgctttg aagcaaccct
    10261 gtacattaat ttattaacag ctgttttcag agggtaaact gacaaattca taacatcagt
    10321 cactctgtgg tgttagaata atattttagt ataatacatg ttttctcctc tgaaaaatat
    10381 gtttttacca atgaaaaatc aatgtaattt attgttttta cctctgaaaa taccatgttg
    10441 acagaagctg ttatattgtt aataaccgaa gtcattaaag aagtaataaa gtttttacaa
    10501 gggaaaagtt gttgattttc tctgaatctt acagccaaac ttacatcaat aatagcagta
    10561 aggttatcac tttatagact caaccaacac ttggatttta gcgtttagca gcacataaca
    10621 attgttttaa ctaatgaaaa aagcaatatt tattacactc acatgtgttg ttgccctgtc
    10681 aacggtcaga ggtcaggata tgtggggaat agccaatagt aactatgcag ggaattttgc
    10741 catagatatt aatccatctt caatggctgt ttctcctata aagtatgagc tgaatatact
    10801 ttcggggaat atgacttttt ataacaatta tctttattta ccgaaaggta aaatgtctgt
    10861 agggaaattt tttaaggcag actattttaa ctacgattgg caagatcaat atacaggcaa
    10921 ggataagaat gcattttttc agacaggtat taaaggtcct tcgttttttc tgcgctcagg
    10981 acagacagct tttggattac atacaggatt aaaggcaatg ggaagtgtaa ggaatgttga
    11041 ctttgaaatg gcaaagttgt tttatgagaa ttctacctac acacctttgc agcatacaga
    11101 acttgaagcc ggcaataata agttggctgc tttattatat ggtgaaatag gtgccagttt
    11161 tgcacagcag attgaagcag ggcccggaaa cagaatagca ataggtgcta cctttaatta
    11221 cctcatgggc tttgatggat tttatgcaca gaacgatcac atgcaatatc gctttgatga
    11281 tgaatctatg ttgcacgtaa ccgaccttca ggctgaatat ggttatgccg ttccgcaaga
    11341 cggcaaaagc gattacctaa aagttcgtgg taagggattt agtactacat ggggtgttca
    11401 atatatacat ggactgaacc ctgaggctta taacgtatat gctagccgcc tgaaatttaa
    11461 gaagtataaa tataagatag gtatgtcatt agttgatgcc ggaagtatat tatttgcaaa
    11521 agatacccgt acagtaaagt tcgacaataa taatatagac tggagcggat ttgatacaac
    11581 aggagttgaa agcatcagtc aacttggaga acttattcgc tcaaaagttt ttgatgactc
    11641 cacatcagga tatgtggcca caaagtaccg tgctaaacta cctgcagcta tcagtattca
    11701 gtttgattac tgcctgactc attttcttta tctgaacacc tcaataattc aacctgtcac
    11761 cagtaagtca acagctatca gacgaccggc acaattgtct tttacacctc gattagaaac
    11821 atggcgttat gaaatttctt ttcctctaac atggtatgag tatgatcagg tcagacttgg
    11881 aacatgtctt cgtttaggtc aggtagttat aggttccgat tatcttactt ccatgctggg
    11941 tttgtctaaa tttaatgggt tcgacttttt tgttggaata aaacacagca gttatgccat
    12001 ggataagttg agtaagcgta aaaaagaaaa agttaaagac acttataccg gaggcgacat
    12061 ttaaaaacta cccattgctg ccattttaat cgtttaagaa aagtagttca cattacttac
    12121 ttttgccctt caattcaata ggcgtgaaca ttaaacagac agaacagttt ctgaaaaagg
    12181 ttaagggaat gcatacctta gtaataggtg atgtaatgac tgatgcttat ctgtggggtc
    12241 gtgttgaccg tatatcgcca gaagcacctg tgcctgttgt ggctgttcaa aagcgcgaaa
    12301 gccggcccgg aggagctgcc aacgttgcag taaaccttca ttctttgggt gcaatacctt
    12361 ttatatgttc tgttgttggg aaggataatg caggaaaaga attccgcgaa atgattacca
    12421 aaagtggtat gcctgcagat ggaatattag catcttcaaa aaggactaca accataaaga
    12481 ccagagttat aggcaacaac tatcagctat tgcgtgtaga tgcagaagta gccgagccac
    12541 tgtcgcaagc agacggtgaa atgctcataa aaaaagcaat agagataatc agaaaaaaaa
    12601 agccggccgt tatagttttt gaagattatg ataaaggtgt tcttaataaa actgtaatag
    12661 aagccattgt tgctgaagca cagaagcaaa atattcccac aacggttgac cccaaaaaaa
    12721 ataatttttt ccattatcgt aatgtaactc ttttcaagcc taatctaaaa gagctgcgcg
    12781 aaggactaaa gactgatttg gagaaaccaa cggcaacagc gcttacgaag ttggcatctg
    12841 attttgcaac taagcaaaaa attaagtcat tgctgattac gttatcagaa catggagtat
    12901 attattattc aaagaatgaa aaaggaatta tccctgcaca cttccgaaat atcagtgatg
    12961 ttagtggtgc aggcgataca gtaatcagtg ttgcatcttt agcaattgca gcagggtttt
    13021 catgtcatga aatggcggca ataagcaatc tggcaggtgg cttggtttgc gaaaaagttg
    13081 gcgttgttcc tattgatgga aatcagttgt tgaacgaggt taaaagactt tccggaaaat
    13141 aaaatgcaag ttacgcccta taatacagga gaatcaaaga aagagcaggt gcaacacatg
    13201 tttgatgccg tagcacaccg ttatgatttc ttaaatcact ttctgtcatt aggcattgat
    13261 aaaatctgga gaaaaaaaac tattaaagag attggtaaac taaaaccaag gactattctt
    13321 gacattgcca ccggtacagc tgatttagca atagcagcaa catctcttaa tccagaaaga
    13381 ataataggag tggacatttc agaaaaaatg cttgcagcag gaagagtaaa aatagaagca
    13441 aagaacttga acaatattat ttcattgcaa caaggcgata gtgagcaact tcaatttgaa
    13501 aatcaaactt ttgatgcagt gatggtagct tttggtgttc gtaattttca aaatctggaa
    13561 aaaggattga tggaaatgaa acgggtactt aatagtaatg gaaaggtttt tattctggaa
    13621 ttcagtaaac caacaatgtt tcctattaaa caattttatg gcatctattc acgttatctg
    13681 ttaccacttt tcggcaagtt gatttccggt gatgcatccg cctataccta tttgcctgaa
    13741 tcggtaaaac attttccttc aggaaatgat tttgcacttg ttttgaaaca gtgtggtttt
    13801 actaatattg aaattaagcc gatgactttc ggaatagtaa cattatacat tgcagcagca
    13861 tgaaacagag ggttatagtt ttttctttct tagttctgct gttcaatgct gatagcttgt
    13921 ttgcacaaaa gcaaaaaatt ctaaacctgc caaagtatga caggcagcgc tttcattttg
    13981 gatttatact tggcatcaat aaaacagatt ttgtaattga cagagttgct tcttttagtt
    14041 tgattgattc actttatact gttgaatcaa aaggtacatc cggttttaat cttcagatag
    14101 taaccaacat gcgaatggga gagcattttg acttacgatt tttgcccgga ctggcatttg
    14161 catcacgcaa tttgatttat acatttgaaa aaggaagacc actcagcaac gttgtgacca
    14221 aaaaggtaga gtcaactttt gtagagttgc ctctggatgt aaagttcaag tcggcacgac
    14281 tgaataattt ccgtgcctat gtattaggag gagttaagta cagcatagat atggtatcgc
    14341 aggcgaaagt aaaagcaaag gataaagaat atgtaaagtt gcaacgaaac gattatggat
    14401 atgaaattgg agtaggatgt gatttttatt tgcctatgtt taagttttca ccggagataa
    14461 agatgtatca tggtttacga aatcttttgg ttaaagacga tttggtttac tcaaaatctc
    14521 ttgatggatt gttttcaaaa atgttcacct tgtcattgac attcgaataa tagaagatac
    14581 tttttatgca aacatttgaa cttgttgttc tgcccgaaga ggcagccagt gagtcggtaa
    14641 ttcaacacat agttgctaag aaatttggtg gcaacagatt gttccgtatt ataaaacgca
    14701 gtattgatgc ccgctctaag actgtaaaaa tcagattgca gttacaatgg acagatgaac
    14761 agaatcaaga aactttagtt gaaaatcgaa agtggcggca agttgccaat agtgaacctg
    14821 taataattgc aggatgtggg cctgcaggat tgtttgcagc catccgactt attgaatgtg
    14881 gatttaaacc aataataatt gaacgaggca aagacgtacg taatcgcaga cgtgaccttg
    14941 ctgcaataaa taaacaaagg cagattaatc cggagtcaaa ttattgtttt ggcgaaggtg
    15001 gtgcaggcac ctacagcgat ggaaaacttt atacacgttc caataagaga ggcaatattg
    15061 cttctgtatt aaatacattt attgagcatg gtgccgatgg tgatattgct gttgatgcac
    15121 atccacatat tggaaccaat aaacttcctg caataatttc tgcaatgcga aacagaattg
    15181 ttgaatgtgg aggcgaagta ttattcgata caaaacttgt aaatattgat tttgacacaa
    15241 gtggtgtaaa tggagtagag gtgcaacaag ggttggcaca atttcatatt aaatctaaag
    15301 ctctgatttt agcaaccggt cattctgcac gcgatgtttt ttacatgtta aacaatcaaa
    15361 aaatattgat tgaagcaaaa ccaattgcta ttggtgttcg tgctgaacat cagcagcagg
    15421 ttattgactc tgtgcagtat cattgcagta aacgcagtca atttttgcca ccggcatctt
    15481 atgcattaaa acatcagcat gatggtcgag gaatttattc tttctgcatg tgtccgggag
    15541 gcatcattgc acctgctgca accgacattg gcgaagtagt tgttaatggt tggagtcctt
    15601 caagaagaaa taatgttttt gcaaattcag gaatcgttgt tagtattaca gaagaagatg
    15661 tattaccatt taagaatgca aaagaattac gttttttaga attgcagaaa agtatagaag
    15721 aaaaagcatt tgttagtggt ggaagtaatc ttgctgcacc tgcacaacgt atcgttgatt
    15781 ttgtgaatca aagagtgtct tcaaatctgc ctacttgctc ctatataccg ggaatcaatt
    15841 cagtaaatct gaaagaaatt tttcctgctt cggttacaaa ctcgcttgct gcagcatttg
    15901 ttgcgtttgg taaaaaaatg aaaggctact atacaaacga agcagtattg gtgggcacag
    15961 aatcacggac ttcatcaccg gttcgtattc ctcgtgatgt tgaaacagca atgcatgttc
    16021 aggttaaggg actttttcct tgtggtgaag gtgcaggcta tgccggaggc attgtgtctg
    16081 ccgctatgga tggcgaattt tgtgcagaaa aatgtgctgc atatctaaaa aataataatt
    16141 gaattagtta cttttaaatc cacattcttt aacaatggaa atcaatcaac caccttacta
    16201 tatcagaata acttttaaaa tcctgttagc gattttaatt ttttatgtcc tgcatgctgc
    16261 acaagatgta ctcattccac ttactgttgc tgtattgttt acctttttgt taatgcctgt
    16321 ttcaaacaaa cttataaaat ggaaatttcc acctgccata gcaattttat gcagcatttt
    16381 acttgcattc gtagccgttg gtggactttt gtatttcttc tattcgcaaa ttgtttcatt
    16441 tgctgaagat ttgcctttac ttcaggcaaa actccttgaa aaagtagagt ttttgcagcg
    16501 cttcgtaaac gaaacattca gaattaccaa agagcaacaa aatgttttgg taaaatctaa
    16561 attgcaggaa aatattgcag atagtggatt actgttgctc aatctttttt cttcaacagg
    16621 aacttttatt gccaattttg ccttagtaac tgtttatgtt ttttttctga cactttataa
    16681 gaaaaagttt aagcagttta ttgaagcaat cgtttctgat ggaaaacaca attcctattt
    16741 tcagattgta aaaaaagtag ctggtgtttc acaaaactat cttaaaggta tttttttaga
    16801 tgttttgatt ttatccattc ttaattcaac aggatttttg ttattgggca ttgaacatgc
    16861 tgttttattt ggagtactgg cttctgttct caacatcata ccctacattg gtgttttaat
    16921 aggaagtgtt ttaccggtac taatggcatt gctcacaaaa gatgaaatag gctatgcaat
    16981 tggagctgcc ggagtttgtg tcactgtgca gtttattgac aataatttta ttactcctta
    17041 tgtagttggc agtagtgtaa gtataaatcc actcactgca acaatagcgt taattatcag
    17101 tgcttcagtg tggggcttgg caggaatggt tttatgcctg cctctgacag ggatgttaaa
    17161 ggtagtttgc gaccatgtag aaggtttaaa acctattgga tttttgatag gtgaggaggt
    17221 ggattatagg aagaataaat ttaaacttgg gcgaaaatag gtctgttgaa atgaaatgct
    17281 aattttgcaa tttatagcga attggcatga gccgattcag attaaaatca agagtaaaaa
    17341 tggagaatct taaacagcat attgaagcat taattttcac ttcagaacaa agtatcactg
    17401 caaatgaaat tgctgctgct ttgaaagcaa gtttcggatg ggagctttct aatgaaaata
    17461 taagacaaag tatttctgaa ttaatcaaca aatattctgc tgatgattat tcttttgagt
    17521 tggtagaaat tgccggtgga tttcgttttc taagtaagaa ggaatattac ggtgtagtga
    17581 acaccatgtt gcagataaac tctaaaaagc gacttacaac agcagcattg gaaacgcttg
    17641 ctatagttgc atacaagcaa cctgtttcaa aatcagaaat tgaaaacatc agaggcgtta
    17701 actgcgatta ttccattcag aaattattag agaaagattt aattaccatt cagggaaaag
    17761 gtgatggccc cggaaaacca attttgtatg gtatcagccg aacattcatg gatcatttcg
    17821 gtttaaaatc agcaaaagat ttaccaaagt taaaagatgt aattcttgat gagaacgaga
    17881 ttggaacacc tgccgatatg cagtatgcag agaacgacaa tgccgaaggt gtaagtatta
    17941 ccttaggaac acaggaaggc aatacagaca agaccgatga cgttgcagaa taatcagtct
    18001 ttaattgaaa ttcatttttt gaggtgaaaa tttctgtact tggcttgcaa tttgattcgg
    18061 cattaaaaaa tgttttcagc atgttatcaa ttgcactcat ttgtttaatg caaccaagta
    18121 tcagtgaagg tcaggagaaa ttacgtaagg tagaaaatca ggcatttact tatggcgaag
    18181 ttttggaata tcgagtacat tacggaataa tagatgcagg cgaagcaaag cttgctgtaa
    18241 tgcctgataa aaaaactata ggtcctcggg aagtgtatca tgtagttgga acaggacaaa
    18301 caaaaggagc attcgactgg ttttttaaag tgcgagaccg ttatgaaagt tttatagatg
    18361 cacaggcaat tatcccttgg ttgtttgtca gaagggttta tgagggtgga tataaaatca
    18421 atcaggatgt tacttttaat caggtaaaaa atttagccat aagcaagaag gcaaccattg
    18481 ccacaccaca gaatgttcag gatttgattt ctgcatttta ttatgcacgc acaattgact
    18541 tcagcaatgc ccaacccggt gccttatacg atgttgatgc gtatttagat gacgccatta
    18601 ttcctgtaaa attcaaatat gtaggaaaag aaaatatcag tactaagttt ggcactttcc
    18661 gttgtttgaa gtttcacccg caattgttgg aaggccgtgt ttttaaaaac aaggaagaca
    18721 tgactctctg ggtaagtgat gatgagaata aaattcctat acgagcagaa gcaaaaattt
    18781 tagttggatc tgttaaaatg gacattaaaa gcttttcagg acttgccaac gaaacactat
    18841 cgttgttgaa gaaatagttt aataacattt cgaactaact ttaataaata tctaagtgtg
    18901 atttgttgag agaagttaat ttatttgata atcagccacg gctgacaggt ttgatgatta
    18961 gataatttga taatgttttt gaatttttga atgatgttgg tttccacaga gtattcagat
    19021 gtaaaattgt agagacagag atgcggaaaa aaagtgtttg ttaaattaca caatctgggg
    19081 ttttgcaact tgacagttat aaagttaact acttgacaaa aggaagaaat tgttagtcgg
    19141 tgtttatgta caaaggtgaa ttcccactca gtttactaag ttgctaactt ttgtaaacta
    19201 aaacatatta gctgaaatta ctagctttca ttgctttatc actttacctg ttttaaacac
    19261 acccttgtag ttgtgcaacg cataataata aataccttct ggaagctgct ctgtatttat
    19321 agtcgtggag tttgaaaatg tctgttgcaa aactcgttgt ccaacaaagt tataaagaga
    19381 cgctgttgtt ttttcgttgc tggcaagaga aaaagtaagt tgactggaaa agggatttgg
    19441 atataaactt acatcagcat ttaaaacctg aagagtattt tgattttgaa aagatggaac
    19501 agacgacact aaagggcata aactgctgtc agtaaatgaa attgagttta ctgacatttg
    19561 tgccagcaca tcaatactta caaggcttac cggttgagat aaaatgcttg tggcactatc
    19621 ggttaccgaa tacgaataag ttgtaaaggt ggatggtgtg ctcatacaag gtgttgttag
    19681 aacgctatca gttttgcaaa agtaaaatcc aaaattattg aagccacctc cataaccaaa
    19741 catcgctaaa ccggtttgtg ggttgaagta atggctgtat gaatgacaat ttgagtaatt
    19801 attatactgg tgcatggata acaggtttcc tgcactatca attcttgctg ctgcatttat
    19861 cgaaggatta aaaatgtcaa aattacctgc tgtaacaaaa gtgttgcctg aatccggtgt
    19921 tatttggtcg aagaaaagat tgtaaaaagt ggaatctgtg aaaccaacat tccatagcgc
    19981 atttccgaca ctatccgttt ttaaaaactg tgcagagccg ttaaaggcgg ttgctacggc
    20041 aaacataaag ccatggtcat gggtttctac tacggaagaa ggaataatat tttattgaca
    20101 taattaagct ggcgttgcca tgtaatattg ccaatactgt cggttttgat aagtatggtt
    20161 gaagcgggta atattgtgcc ataaatttgc cgggttcctg caaggagaaa accaccatcg
    20221 ctggttagag ttaatgccct aagctcaatt ctgcgtgatg tacctccgtt catcattctc
    20281 gaccaaataa tgttgccatc taaatccatt ttacagaata gccctccatt aactccgttt
    20341 gctgaaacac gtcctactcc ataaatagca ttattttttt cttcctgaaa ataaacagtt
    20401 gatgaaatgt agttaagttt tttggcacct aacatggtac ctgaataact tgttttaagt
    20461 tgaatagtgc ggccatcgtc atagaggtta agcattaagg tgctatctga aagaggtaga
    20521 acagatagcc cgaatgtttt aaaaattgcg gtaactcttt gataagattt actccatttc
    20581 agtatgccgt cattacccac aagagaaagt attgcacccg aatctggacc tccaaaagta
    20641 ctgagagtat aataccccga atcatcgggc gattttataa catcataata agtgagaaaa
    20701 atgtcagaaa caccactggg tttgaacatt ttttcgaaag ccgtctgtcc gctggacaag
    20761 tatgttgcaa gaataaaact gaattgtaaa agttttttca ttgtctaaat tttcgataaa
    20821 cgattcactg tagtggtgtg ttttaacact gtcagtgtaa ctcacaaatt tacgaagttt
    20881 gatttttaaa accaagcaaa cgatactact tttcaatctt atagcatctc aaagttttat
    20941 aaaaatagtg cataattata tttttaattt ttgaggatat aaagaaagtt tgaaggttat
    21001 aatgttttta aagtttgtgt tatttctaca gagtttcgcg aagtaatatc gaagagaatt
    21061 tgaggggata aaaattaagt atatgtttct gttaattcac tctgtcaggg gctgtgctac
    21121 ttgacaggtg aaatcagaag gttgcaatgt tggaaggttt agaatgttgt ttgataagta
    21181 aaaaaaacag tctttgagcg acaaactttt ggtggaaaaa tagatatcaa aaacttttaa
    21241 agtgccatag gtacaacctt attattggta attaaataac acaatagtaa tataagtttt
    21301 ttaatgataa ttaagcacaa gtcgaaagac ttgagccaga tgggagaaat aaattactca
    21361 acaattaatt tcttgcagtt gctatttttt ccatcaaaca ctttcatgaa ataaattcca
    21421 tttgatatat tattcaaatt gataactttt tttgattcac ttataatatt ttcttcaata
    21481 atcttattgc cgaaagtatt gaacatctga attttcccat tgtcaattac atgattgaat
    21541 tcaattacaa cctgacttaa tgcagggttg gggtaaatgt tgatggaaga ataataattt
    21601 gtgttttctg taatatccgt caataagttt gaattgagaa aaactccatt cccttgtgta
    21661 gcagcaaaaa aatttattcc gcttattgtt aaatccgtaa caattttatt tgttaggcca
    21721 ttattcacag ctgaccatgt agtgccgttg ttgttagaaa aatacacacc atcttgcatt
    21781 gtggctgcta aaatatttgt tccattaatc gcaagacaag taataattga tgtggttggt
    21841 aaaccattat ttactgcatt ccacgagttg ccattattgg atgaaaagaa aacaccatta
    21901 ctatcagtac ctgcaaaaat atttgtcccg ctaatagcta aagaagaaac atgcagattt
    21961 gttaaaccat tattaaccgc attccatgta ttgcctgtat cagttgaaag aaaaacaccg
    22021 ttttgataag ttcctaaaaa aatgtttgag ccacttatta ctaaacttga agagccgcct
    22081 gttggtaaac cattgtttat cagattccat gttgaaccat tattaacaga taaatagact
    22141 tcatttaaat aagatgcagc aataagatga gaacctaatt tagcaacagc tattataaat
    22201 ttatttgtaa taccactatc ggattgaacc caagtatttc cagtgtcagc agacataaaa
    22261 agtccgttaa ctgttccaac aaaaaggtca ttagtagtag ctaggataga attaataaaa
    22321 gtgctggtta aaccattatt aatctggctc caagaatttc cattattagt agagcgatac
    22381 attcccttat aattacttcc tgcaaataaa tttgaccctg acacagctaa tgaatgtacg
    22441 aaagaacttg ccaagcctgt attttgtgca ttccaggaag caccattatt agttgataga
    22501 aataaaccat ctgcagaagt gccaacaaag atgtctgaat tatttataag aaatgaataa
    22561 attgttgaac ttgttaagcc attatttgcc gaagtccatg tatttcctgt gtcagatgaa
    22621 aaaaatacac ctcctccggc agttcctgca aaaacatttg caccacttgc tgcgattgca
    22681 gaaacgtgca gatttgttaa accattattt aaggcattcc atgtatttcc agtatcagtc
    22741 gatagaaaaa cacc
//
LOCUS       DAOOOL010000026        22655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_173_length_22655_cov_79.618009, whole genome shotgun sequence.
ACCESSION   DAOOOL010000026 DAOOOL010000000
VERSION     DAOOOL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22655
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_22655_cov_79.618009"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..915
                     /locus_tag="PKU82_03710"
     CDS             385..915
                     /locus_tag="PKU82_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82012.1"
                     /translation="MAYIRNTGQNNRFWVKNEFGFDKDKPWLTRPLDKPFIYKIDSVF
                     WQNPDSILVNTIKSTFKFSPFNKEWWGTYSIKNNTIKIEYFSKTNQESIIHNRYLIEL
                     SGYLKNDSTIIITNRICGKWYHCYQCKSDLKNNLNSYTPPVQYNFYSTDFKPDSTQAW
                     FLKKHWYKKGLHQSRK"
     gene            1345..1881
                     /locus_tag="PKU82_03715"
     CDS             1345..1881
                     /locus_tag="PKU82_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82013.1"
                     /translation="MKIPIKLKGYYSQLEHIETNVGYPPNVKKVVDTIARNVVFYEDG
                     VFLYSFYGEQFKGNNPIGYKGGVALWGGYSIFNDTLKGFFMTGPDSRPNRPWGYVWFK
                     IIDKNTIRKICFKWQEPITAADVFEFKNTRAKNISNATFIPSDSLPNPNLSWIKNKKW
                     FWCDEQQFKLWKKAQKEK"
     gene            1972..2103
                     /locus_tag="PKU82_03720"
     CDS             1972..2103
                     /locus_tag="PKU82_03720"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82014.1"
                     /translation="MEELAKDGAILEHARNAVINILNDDPLLNSEENKIFYRHLHQL"
     gene            complement(2130..2327)
                     /locus_tag="PKU82_03725"
     CDS             complement(2130..2327)
                     /locus_tag="PKU82_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82015.1"
                     /translation="MHALDILLLGGAVVCFVLAYIIASRPARELRQSNKSKSKWNHVN
                     MDSASSVHSPEMMKYVHDKTG"
     gene            2819..3763
                     /locus_tag="PKU82_03730"
     CDS             2819..3763
                     /locus_tag="PKU82_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ82016.1"
                     /translation="MMSKEILLIDTFHETFIPKLTAMGFHCTEGYEWSLEKIQQSIGN
                     YEGIAIRSRIPLNKDLLQHATNLKFIARGGAGMENIDVAYAVSKNIKCLNAPEANRDA
                     VGEHALMMLLALLNKLMKSDKEVRNGIWLREENRGTELQGKTVGIIGFGNMGSAFAKK
                     LAGFEVNIVACDPYKKIDTAEYPFIKQVSVEQLQQESDIISLHVPLTAETKYMINDTF
                     ISQCKKEIYIINTARGKCLNTADLVKHLKTGKVKGACLDVHEYETSSFEKLETLPEPF
                     GYMIKSDKTILTPHIAGWTHESNRKIAEVLVKKIEQLS"
     gene            3879..5285
                     /gene="glmM"
                     /locus_tag="PKU82_03735"
     CDS             3879..5285
                     /gene="glmM"
                     /locus_tag="PKU82_03735"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOZ82017.1"
                     /translation="MTLIKSISGIRGTIGGKSGESLTPVDVVKFSAAYATWLAAQFPA
                     KTKCKVVLGRDARISGPMIHNLVCGTLAAMGIDVIDVGLSTTPTVEMAVTFHQAHGGI
                     ILTASHNPKQWNALKLLNSKGEFISAADGETLLKIAEQESFEFANVNQLGTVTTDAHA
                     IDKHIAAILALPLVDVKAIASKKFKIVIDCVNSSGGIALPPLLKALGVEEVIELYCTP
                     DGQFPHNPEPLPEHLTELSKTVKKSKAHLGISVDPDVDRLALVCEDGEMFGEEYTLVA
                     VADYVLKNKKGNTVSNLSSTRALRDVTEKAGGSHYASAVGEVNVVEMMKAKNAVIGGE
                     GNGGVIYPDIHYGRDALAGIALFLTHLAKYGNTVSMLRSSYPDYFISKNKIELTPEIN
                     VDGVLESIQEKYKKHKINTIDGVKIEFDKEWVHLRKSNTEPIIRIYAESDSITTANNL
                     AEKIISDIKELISHPAKV"
     gene            5317..6456
                     /locus_tag="PKU82_03740"
     CDS             5317..6456
                     /locus_tag="PKU82_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOZ82018.1"
                     /translation="MKVYFDNAATTPLRQEVIEAMLPVMQKIYGNPSSIHSFGREMRS
                     AIEKARKTIATLLKVAPSEIFFTSGGTEANNMAIRCTIHDHKITHAISSPIEHHAVTH
                     TLEDLEKEGIKLSWVKLNDKGEADYEHLEELLKNNPRSFVSLMHANNELGTVNDIDRI
                     GELCKQYNAIFHCDTVQTMGHFPYDMSQKNIDFFTCAAHKFHGPKGVGFIYINHKLKI
                     HPLITGGAQERNMRGGTENAYGIIGLAKALELAYAELEEDRKHIQGLKEYMKQRLQEE
                     IPGVAFNGDVSARSLFTVLNVSFPPYEGKDMLLFNLDISGIAASGGSACSSGSEIGSH
                     VLRGINADPERPAVRFSFSRYNTKAEVDFCMDKLKALFAVTAVVK"
     gene            6467..6967
                     /locus_tag="PKU82_03745"
     CDS             6467..6967
                     /locus_tag="PKU82_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOZ82019.1"
                     /translation="MNLVCLPEEVERLASLREQETLDYFKSIKKKYKSAVIDKAVHLL
                     YAEIAPKIDCTACANCCKKQSPVFTEEEIRPILNHSGISEHENLNELLEHDEINHHYI
                     LRKNPCFLLNKNLCSVYTLRPSSCSEYPHLNRPFFIYRNKSNMSNYKICPIVYNVVEA
                     LKNSLS"
     gene            complement(6984..7151)
                     /locus_tag="PKU82_03750"
     CDS             complement(6984..7151)
                     /locus_tag="PKU82_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82020.1"
                     /translation="MNLFKPQNDSQVSLLEDIKTLRSLKGNTSRFAILRSVAKTKKHT
                     RTVYPEGWKFE"
     gene            complement(7296..8495)
                     /locus_tag="PKU82_03755"
     CDS             complement(7296..8495)
                     /locus_tag="PKU82_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82021.1"
                     /translation="MFGIGKRIHLVPLGVFLTIFMFIHGVSTAQTKKTLTINAKFKID
                     GGGSLGDAYMVLQRDGQSIETLPGQSSYTLNLEIGADYILSFNKPGYITKKININTTG
                     ADEERVEQGFESYPFTVVLMKQYEGVNIVVFNQPVAKITYSKKYDEFDYDTDYTKSIQ
                     SAIQKAEDDLKKAEKEFKKNPPKPAKDSTIVANKPKAEEVKPQKEKTKTAPQEEPVAV
                     KEKGKNDDTKNESKNSNKSAAATGGQDQPLKPTQGGGSDEPEGNIDIEKGADQKADIE
                     PTAGNDAIKNKIGLTKGADQPPAAEPAKPQPVVIKTPETAAPEKPKEVVVHTAPEPPE
                     NVSVRREQLAEDRKTVYNVWVTKGNKTVLYSKVVYAWGGIYFFRDLNTSISENMYKQA
                     DAGIVNF"
     gene            8582..9070
                     /locus_tag="PKU82_03760"
     CDS             8582..9070
                     /locus_tag="PKU82_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82022.1"
                     /translation="MLVVYDRINKEFSKRRKFFLLLLMLSVFSPALRAQENITVQQTL
                     DYINQKFAGKYVVSINYGVLIATYFDGETKYREDQAAIKDLDATKVYYNSKAGMLLIN
                     CTNPQKKCVTREFLGKRLFYSRISFPVSYGQKTNDGLVKAFQHLIHLINDKNYENSEP
                     FE"
     gene            9045..9599
                     /locus_tag="PKU82_03765"
     CDS             9045..9599
                     /locus_tag="PKU82_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82023.1"
                     /translation="MKIQNHLNNVKILFIVLLSAFCVQANAQVDSADFKMRIDSLMNQ
                     GKIKFNNGKFQEALLDYTYVANMDVANVNAILQQGFCNTILKKYTEAIGNFTDVIFYE
                     PNHEWAYVSRGSAYNKIEKYDKAIVDFNKALELKPEDAEAYNNRGFAKKMLGDIKGAC
                     EDWNKSKKLGNEEAKIILKNNYCK"
     gene            9596..9994
                     /locus_tag="PKU82_03770"
     CDS             9596..9994
                     /locus_tag="PKU82_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82024.1"
                     /translation="MIKVREFVFLILIGAVVLLSAFKIQQDGKDYGQCLQKVNKKFGA
                     ETLSCPASTKPYKVWFVNTCSDTLAVKLAVQEKSKRWRTFTRTQLLPGDTISGYACTG
                     VGKYLYYTKQYKDNSVDLPSDEVIDNSNKE"
     gene            complement(10024..10887)
                     /locus_tag="PKU82_03775"
     CDS             complement(10024..10887)
                     /locus_tag="PKU82_03775"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOZ82025.1"
                     /translation="MSLHTVHAQELNCQVNVMAPQIQNVDKKVFETLQQSIFEFMNNT
                     KWTSDKYLNQERIECSVTITINERVSNDEYKGSIQITSRRPVFQTSYNSPTLNFNDDN
                     FQFKYIEYQPLEFSENGSNPNLTAVLAFYANIILGVDYDSFSLYGGAPYFAKAQTIVN
                     NMQNAREAGWRAFEGTKNRYWLSENMTNPIFKPYHEMFYQYHLKGLDVMAKKKDDGLS
                     QISESLDLLMSVHKEKPGSLLLQTLFYAKADEIVNIYSGAYPDQKVKVSNLLNEIDPA
                     NGTKYSKIMGN"
     gene            complement(10917..12122)
                     /gene="coaBC"
                     /locus_tag="PKU82_03780"
     CDS             complement(10917..12122)
                     /gene="coaBC"
                     /locus_tag="PKU82_03780"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOZ82026.1"
                     /translation="MLQGKKILLGVTGSIAAYKTPELVRQLVQQGATVKVIVTAAAKD
                     FVSPLVLSTVSKNETLQTFTSEDNSTWNNHVELGLWCDIFLVAPLSANTLAKFANGIC
                     DNLLTAVFLSCRNTVMVAPAMDVDMFKHPSTQKNLEIIAKNNIKIIGPESGELASGLT
                     GKGRMTEPEDIVQEVINYFNPSLPLRNKIAVVTTGPTQEALDPVRFIGNHSSGKMGVA
                     IANCLAAKGASVILISGPSVSLGNLNKSIKIVHITSAAEMLKACKQAQPDASIMVMAA
                     AVADFRPEGISDQKIKKKNNEGLSVQLVPTEDIAAEMGKLKKEGQILVGFALETNDEK
                     QNAERKLKNKNLDFIVLNKLEESNQVFGSDFNRITIIDKNLVTTPFEKKSKDAVASDI
                     VEKIITLVQ"
     gene            complement(12127..12465)
                     /locus_tag="PKU82_03785"
     CDS             complement(12127..12465)
                     /locus_tag="PKU82_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOZ82027.1"
                     /translation="MNSRNVAQTTVTRDVVDFEKKTENIYESLAIIAKRANHIAAETR
                     EELHDKLSEFASSTDTLDEVFENREQIEISKYYERLPKPTLVAIQDFLDDKIYFRSPA
                     KEENKDSAEN"
     gene            complement(12475..13287)
                     /gene="bamD"
                     /locus_tag="PKU82_03790"
     CDS             complement(12475..13287)
                     /gene="bamD"
                     /locus_tag="PKU82_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOZ82028.1"
                     /translation="MKNRLSVYFLLMALLLTLGSCSKFNRIRKSGDMNMKYDAAVKYF
                     ENKKYFNALQLFEELIVVLKGTDKAESSYYYYCLSYFYTGDYTSAAYHFNNFAQTFPM
                     SAKAEDALFYNAYCYYLDSPPSSLDQQSSLDAIRQFQLFINKYPTSQRVAECNKYIDE
                     LRSKLEVKAWDNAMLYYKMEEYKSAIVAFENVVKDFPTTKYQEECLFLSLKSAYLYAQ
                     NSIESKKEERYKSTIDHYNKLIEIFPATEYKKEASRILSAAEEKLKDYHTVN"
     gene            13440..16946
                     /locus_tag="PKU82_03795"
     CDS             13440..16946
                     /locus_tag="PKU82_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ82029.1"
                     /translation="MLKRFLFIMLLCTTTMAMAQTDYRLMFLGKAVLPAENLNSFIQE
                     KVSENDVYNGYYYRIIQFYTVPTQAEKAVMETAGIKLLEYLPKNAFNAAIPISFNKQL
                     LSGFNVRSVTNQSSEQKLSISMLGDYPKYAVPSAGMVDVRLQFQKNISIQQAQQIAAT
                     YGKILSSTEINNMVEVRMQQQSVATISQQPWLFFIDLTSPPSVKDDLEGRSLHRSNVI
                     NNDLLMGRKYNGSGVAAALADDGFVGPHIDFTGRITNYTTTVGPSHGDMTGGILAGAG
                     NLNPAYPGMANGVQLHVFDIGSYPQIIDAVANYNNLGTVVASTSYSQGCNEYTTDTQF
                     GDQTIHDNGQLEFVFSAGNNNGTNCGYGAGGNWGNITGGYKQGKNVIAVANLDALEVI
                     DPSSSIGPASDGRVKPDIAANGRDQMSTDENNTYQVGGGTSAACPGIAGICTQLIQAY
                     KQINSATDAPTALIKACLLNGAEDIGNPGPDYKYGWGRVNALRAVTTIEDARYFTDSI
                     NQSDSLTYSINVPAGTSQMRVMVYWHDVGGSPASSTYLVNDIDMQVTDPSNAVWNPWV
                     LDPTPNAANLNAPATRGIDHLNNMEQVTLDNPAAGNYVVSLKGFAIPQGPQTYYVVYE
                     FRNDDITVTYPMGGEGFVPGHPEIIRWDALKGLGSFDVEYTTDNGTTWNNIATVNQNT
                     LQYSWNVPNNIATTGDARVRVTRGSVSGLSDTTFTIVGIPQNLNVIYSCPDSIKIGWA
                     AVSNAAWYEVSMLGTMYMDSVATTTNTDYIFTNLIASNSYWFSVRAVMANGNKGRRAN
                     AIKKQLGIFNCPNIPPSVQFNANFNVGCTSKTFTFTDQSSNAPSSWNWTFNPNTVTFV
                     GGTSATSQNPQVQFNTPGTYDVTLDATNGIGTSSSTQTAMINILAPSLPPMTQDFQAT
                     VFPPANWSIDSSNANYTWLKSALITGSAGTSTYAARFNNYSYNSSGSEDGLISYEVSL
                     SNAAAALLTFDVAYAQYSSVYNDGLRVDISTDCGNTWVPSGYLKTGVALATTGTTTSN
                     FAPNAANQWRNDSVSLNAYLGQDVSIKFVNINDFGNSMYVDNVNLDVTTGLNQLDNNV
                     GTVNISPNPSDGKFIIYSKQTSHESYTLTVYDINGKVIAEDVFPANNKSHAVDISNFS
                     KGVYFIKVEGEKSVQRLKVISR"
     gene            17153..18241
                     /locus_tag="PKU82_03800"
     CDS             17153..18241
                     /locus_tag="PKU82_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOZ82030.1"
                     /translation="MNKNNYCVIMAGGIGSRFWPMSRNAFPKQFLDILGTGQTLLQQT
                     FNRFTKICPIENIYIVTNESYIDLVKKNLPQLPVENILGEPLRRNTAPCVSYAAFKIA
                     AKNPDANIVVAPSDHIITKEDAFIEAINKGLTFTAQNDCLLTLGITPSRPDTGYGYIQ
                     FVDEKHNEINKIFKVKTFTEKPNLEMARFFLQSGEFVWNAGIFLWNLKSILKALKEHL
                     PEMYHLFEEGNSIYNSKGEDAFIKTAYSQCTNISIDFGVMEKAKNVFVMSADFGWSDL
                     GTYGSLYEHITHDANKNAVVGKNVMLYDTKNCIVNMPHDKLVVLQGLSDYIVVEAENI
                     LLVCRKQDEQQIRQFVNDVKSAKGEKFV"
     gene            18529..19650
                     /locus_tag="PKU82_03805"
                     /pseudo
     CDS             18529..19650
                     /locus_tag="PKU82_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            19690..19878
                     /locus_tag="PKU82_03810"
     CDS             19690..19878
                     /locus_tag="PKU82_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOZ82031.1"
                     /translation="MQNGTVKFFNETKGFGFIKAENGEEIFVHSSGLKEQIRENDNVV
                     YEVQQGKKGLNAVNVRLA"
     gene            complement(20103..20834)
                     /locus_tag="PKU82_03815"
     CDS             complement(20103..20834)
                     /locus_tag="PKU82_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOZ82032.1"
                     /translation="MIESLVIIPTYNEKENIQKIIKAVFNLLNTFHVLVIDDNSSDGT
                     ADLVKQLMPQFPESLHLVQRQGKMGLGTAYIHGFKWALERNYQYIFEMDADFSHNPAD
                     LQRLLDACKNGGADVAVGSRYINGVNVVNWPIGRVIMSYYASVYVRLITRMPVRDATA
                     GFKCYRRKVLETLDLSKIKFMGYAFQIEMKFAAWCSGFRIIEVPIIFTDRTEGKSKMS
                     SGIFKEAFFGVIQMKIKSWFRKYTH"
     gene            complement(20917..21360)
                     /gene="dut"
                     /locus_tag="PKU82_03820"
     CDS             complement(20917..21360)
                     /gene="dut"
                     /locus_tag="PKU82_03820"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOZ82033.1"
                     /translation="MQNKTEVKIINKSSHPLPAYESNSAAGMDLRADLKEAVELKPLE
                     RALIPTGIYIELPQGFEAQIRPRSGLAAKHGVTVLNTPGTIDADYRGEIKVILVNLSS
                     VAFTINNGERIAQMVVAAHQQVVWKETLELSETNRGAGGFGSTGK"
     gene            complement(21412..>22655)
                     /locus_tag="PKU82_03825"
     CDS             complement(21412..>22655)
                     /locus_tag="PKU82_03825"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOZ82034.1"
                     /translation="VLLMIFFSSPLAATIEYPGKGNYITWLALIIGFDAITAIPFARL
                     RQQGKALQFALLKIAGIVINIGLNLFFLIGCPIWIKGNDGLLSQLAVTFYSPTVGVGY
                     VFISNVIASGFTMLLLYRQFSNLTWHLDSALIKRMIYYAFPLLIAGFAGMVNETFDRA
                     VYKYLAPDANHALRDLGIYSACYKLSIIMTLFVQTFRYAAEPFFFAQHDKEDKKETYA
                     KVMTWFVITCSFIFLGVMLFIDVIKLFIGEQFRSGIDVVPVLLLANLCLGVFYNLSMW
                     YKLTEKTRYGAYFSIFGAVLTIILLFVLIPSFGYMGAAWATFITYFAMMIVSFITGQK
                     HYPIPYKVKNDVFYVLLSLMLFSLSAYLQHQLDLPGSVKWIVNIILLFVFPFVVFRMN
                     KELLVKKQKVESQQNTIGRRN"
BASE COUNT         7443 a         4159 c         4052 g         7001 t
ORIGIN      
        1 tttcagaagt aaaacttctt tgcacaaaaa cccacgaagt gcaacttcgc gggtacggaa
       61 taatttaatt cctggatggc atggatcacc gtatattatt acagattatg atactccttt
      121 aaaaatgtat gggcacaata agtttaatgc tttttattct ttgtattgat gaaaactgag
      181 attttaagaa ggcaaatttt tattctattg atacccgttt tcattatggg atgctttact
      241 tcacataaaa ggtttaactt atttttcgag aataagactg actcacttag tataaaatta
      301 aaaataaaag gaatctacta tttggcaaat gaaccaatag atagatgtat tccaactgca
      361 tatatattcc tttttaataa tggtatggca tatattagaa atactggtca gaataatcgc
      421 ttttgggtta aaaatgagtt tggatttgat aaagacaagc catggttgac aagaccttta
      481 gataaaccat ttatttacaa gatagactct gtattttggc aaaatcctga ttcaattctt
      541 gtgaatacaa taaaaagcac tttcaaattt tctcctttca ataaagagtg gtggggaact
      601 tacagtataa aaaataatac aattaaaata gaatattttt caaaaacgaa tcaggaaagt
      661 ataattcata atcggtattt aattgaatta agcggctatt taaaaaacga ttcgacaatt
      721 ataataacca acagaatttg tggtaagtgg tatcattgtt atcaatgtaa atcagatctt
      781 aaaaataatt tgaatagtta tacaccacct gtgcaataca atttttactc aaccgatttc
      841 aaacccgata gcacacaggc ttggttctta aaaaaacatt ggtataaaaa aggcttgcat
      901 cagagcagaa aataaaaaac cccgtacccg cgaagttgct cttcttgggc agtgtgacat
      961 aaagctgtgc tttacaaagt gaacaataaa ttatatttac acaataaaaa ataaaggaaa
     1021 cagtatgctt tcagaagtaa aacttcgtta cactaaaacc tacgaagtac gggagtatag
     1081 tagttctttg gtacaagata gaactcaaaa ttgggtgcat aaaaatggtt ttttttattt
     1141 accctttggg aaggctccgt attataaaga ttatagccaa tttaaatctg gcatgtattt
     1201 ttatggcgga tataataatc catactcttt atggggaaaa taagaaattt tattactatc
     1261 ttaacattat tagggttgct attgggttgt caacccacaa gccgcgtatt taaagatgcc
     1321 ttttcttgtt ataaagacac tagcatgaaa attccgatta aactaaaggg ctactattca
     1381 cagcttgagc atattgaaac taatgtgggc tatccaccta atgtaaaaaa agtagtggat
     1441 actattgcca gaaatgtggt attttatgaa gatggagtat ttttatattc tttctatggt
     1501 gaacaattta aaggaaacaa tccaataggc tacaagggtg gtgttgcact atgggggggt
     1561 tattccattt tcaatgacac actaaaaggt ttttttatga caggaccaga cagcaggcca
     1621 aacagacctt ggggttatgt atggtttaag ataattgata aaaatacaat tagaaaaata
     1681 tgcttcaaat ggcaagaacc tataactgct gccgatgtat ttgaatttaa aaatacaaga
     1741 gcaaaaaata tatcgaatgc aacttttatt ccgtctgatt ctttaccgaa cccaaacctt
     1801 tcgtggatta agaataaaaa atggttctgg tgcgatgaac aacaatttaa actttggaaa
     1861 aaagcacaaa aggaaaagta agtactattt attttaggtt ctcaattaaa atttaactcc
     1921 cgtacctgcg aagttgtact tcgtgggcag cgtggactac tgaacctgaa aattgaagag
     1981 ttggcaaaag atggagccat tttagaacat gcaagaaatg cagtcatcaa tattttgaat
     2041 gatgacccac tgttgaactc agaagaaaat aaaatattct atcgtcattt acatcagttg
     2101 taagagcaaa aaaaccggag taaggtcagt taacctgttt tgtcatgaac atatttcatc
     2161 atttcgggcg aatgaactga tgaagctgaa tccatattga catgattcca ttttgactta
     2221 gatttattgc tttggcgcaa ctcgcgtgcc gggcgtgaag caataatata tgccagaaca
     2281 aaacagacta cagctcctcc taaaagtaat atgtctaatg catgcataac ggtttatttc
     2341 tttgttggtg ttggttatac ttatacacct gcataagagt tacccaaaac ctgaagatta
     2401 ttccgtaaac cttacatcat tcaaacaaaa aaacatttct ctttgaataa ctaagatacc
     2461 aatcagcaaa cccggttctc tgtttttaat ggttaaccga taatggacaa tcttcttttt
     2521 gcggcaattt aataaaccat tttagcattg tcaactattt tcttacttcc ttagtgaata
     2581 tttgctgtta tttcagccat ttggatacaa aatttataga aattaccttt gtgctattaa
     2641 cttttattct gtatgagaag ggagattacg aatcaagaaa tgcctaatta gcaattggca
     2701 acaatgacaa ataccatcaa atttgtaatc ttgagaataa agacacaaac ataaagaatt
     2761 aatcagacaa cattctcctg cacattcgga ggatttttat ttcatgtcaa ttttaaaaat
     2821 aatgtcaaaa gaaattttat taatagacac ctttcatgaa acatttattc ccaagctgac
     2881 agctatggga tttcattgta cagaaggata tgaatggagt cttgagaaga tacaacaatc
     2941 cataggcaac tatgagggta tagcaatcag aagtcgcata cctttaaaca aagacctgct
     3001 gcaacatgcc actaatttaa agtttattgc ccgcggtggt gcaggcatgg aaaatattga
     3061 tgttgcttat gctgtttcaa agaacattaa atgtctgaat gcacccgaag ctaatcgtga
     3121 tgctgtgggc gaacatgcat taatgatgtt acttgctctt ctgaataaac taatgaagtc
     3181 tgataaagaa gtacgtaatg gcatctggct gagagaagaa aaccgtggaa cagaattaca
     3241 gggcaaaact gtaggcatca ttggttttgg taatatgggt agtgcctttg caaaaaaatt
     3301 agcaggcttt gaggtgaaca ttgttgcttg cgatccatac aaaaaaattg acactgctga
     3361 atatcctttt ataaaacaag tttctgttga acaactgcaa caggaatcag acattataag
     3421 tctgcacgta ccattgacag cagaaactaa atacatgatt aacgatacct ttattagtca
     3481 gtgtaaaaaa gaaatttaca tcatcaacac agcacgaggt aaatgcctga acactgccga
     3541 ccttgttaaa catctcaaaa caggtaaggt aaaaggtgca tgtcttgatg tgcatgaata
     3601 cgaaacttca tcatttgaaa agttagaaac actgcctgaa ccttttggct atatgataaa
     3661 atccgataaa actattctca caccacatat tgcaggatgg acacatgaat cgaacagaaa
     3721 aattgcagaa gttttggtta agaaaataga gcagttatca tgaaaaaagt ttctgttgtc
     3781 agaaagaata aaatactgat attcaggaaa accaactatt aaattcataa aattaccctt
     3841 tgatttgata tatttgcact gtaaaaaaag caaagattgt gacattaatt aaatctattt
     3901 caggcatcag gggaactatt ggcggtaaat ccggagagtc actcacccct gttgatgtag
     3961 taaaattttc tgctgcttac gccacatggc ttgcagcaca gttccctgca aaaacaaaat
     4021 gcaaagttgt tttaggtcgt gatgcacgca tcagcggacc aatgatacat aacttagttt
     4081 gtggcacact cgctgccatg ggaattgatg tgatagatgt gggcctttca accacaccaa
     4141 cagttgaaat ggctgttaca tttcatcagg cacatggcgg aatcattcta actgcaagtc
     4201 ataatccaaa acaatggaat gcactgaagt tgttaaacag caaaggcgaa tttatttctg
     4261 ctgccgatgg cgaaacactt cttaaaattg cagagcagga atcgtttgag tttgccaatg
     4321 taaatcagtt gggaacagtg acaacagatg cacatgccat tgacaaacac attgcagcaa
     4381 ttcttgcatt gccattagtt gatgtaaaag ccatagcatc taaaaaattc aaaattgtta
     4441 ttgattgcgt taattcatct ggcggcattg cattgccacc attattaaaa gcattaggtg
     4501 ttgaagaagt gatagaactt tactgcacac cggatggaca gtttccacac aatcctgagc
     4561 cattacccga acaccttaca gaactgagca agacagtaaa aaaatcaaaa gcacatttag
     4621 gaatttctgt tgaccctgat gtagatcgtc ttgcattggt ttgcgaagat ggtgaaatgt
     4681 ttggcgaaga atataccttg gtggcagtag cagactacgt tctgaaaaac aaaaaaggta
     4741 atacagtaag taatctttcg tcaacacgtg cgctgcgcga tgtgactgaa aaagccggtg
     4801 gaagccacta tgcatctgcc gttggcgaag ttaacgtggt ggaaatgatg aaagccaaaa
     4861 atgctgttat tggcggtgaa ggaaatggtg gagttatcta ccccgacatt cattatggcc
     4921 gtgatgcact ggcaggcatt gcactttttc tgacgcatct tgctaaatac ggaaacactg
     4981 tttctatgct tcgctcctct tatcctgatt attttatttc taaaaacaag attgaactta
     5041 ctcctgaaat aaatgttgac ggagtattag aatctattca ggagaagtat aaaaagcata
     5101 aaataaatac tattgacgga gtaaaaatag aatttgataa agagtgggtt caccttcgta
     5161 aatcaaatac tgaacccatc atccgcattt atgcagaaag tgacagcatc actactgcca
     5221 acaatcttgc tgaaaaaatt atttcagata tcaaagagtt aataagccac cctgcaaaag
     5281 tctgaccccc atcacactta atcaaacaca caaacaatga aagtttattt tgacaatgct
     5341 gcaacaactc cactaagaca ggaagttatt gaagccatgt tgcctgttat gcaaaagatt
     5401 tatggcaatc catcatccat ccattctttt ggtagagaaa tgcgatcggc aattgagaaa
     5461 gcacgtaaaa caattgctac attattaaaa gtagcaccat ctgaaatatt ttttacttcc
     5521 ggtggaacag aggcaaacaa tatggccatt cgttgcacca tacacgatca taaaattacc
     5581 catgctattt cgtcacccat cgagcaccat gcagttacgc atacgctgga agatttagaa
     5641 aaagagggta tcaaacttag ttgggtaaag cttaacgata aaggtgaagc cgactatgag
     5701 catctcgaag aattactaaa aaacaatcca cgttcttttg tatcgctcat gcatgcaaac
     5761 aatgagttgg gcaccgttaa cgacattgac cgcattggcg aactctgcaa acaatacaat
     5821 gcaatttttc attgtgatac agttcaaact atgggacatt ttccttatga catgtctcaa
     5881 aaaaatattg actttttcac ttgtgcagcc cataagtttc acgggcctaa aggtgtagga
     5941 tttatttaca tcaaccataa attaaaaata catccactga ttacaggtgg tgcacaagaa
     6001 cgcaatatgc gtggcggaac agaaaatgcc tatggtatta taggattagc aaaagctctt
     6061 gaacttgctt atgcagaatt agaagaagac cgtaaacata ttcagggatt aaaagaatac
     6121 atgaaacaac gtttgcagga agaaattcct ggagtagctt ttaatggtga tgttagtgca
     6181 agaagtttgt ttacagtatt aaatgtttct tttccgccat acgaaggtaa ggacatgttg
     6241 ctttttaacc ttgatatatc aggaattgca gcatctggcg gcagtgcctg ttcgtcaggc
     6301 agtgagattg gctcacatgt attacgtgga atcaatgccg accctgaaag acctgctgta
     6361 cgattttcat tttcgcgcta caacacaaag gctgaagttg atttctgtat ggataaactg
     6421 aaagcattgt ttgcagttac tgctgttgtg aaataaattt tctcttatga atcttgtctg
     6481 cctacccgaa gaagttgaac ggttggcatc gctgcgtgaa caagaaacgc ttgattattt
     6541 caagtcaata aaaaagaaat acaaatcagc agttattgat aaggctgtac acctattgta
     6601 tgctgaaatt gcaccaaaga tagattgtac ggcctgtgct aactgctgca aaaaacaaag
     6661 tcctgttttt acagaggaag aaataagacc tattctgaat cattcaggca ttagtgaaca
     6721 cgaaaatctt aacgaattgc ttgaacatga tgaaataaat catcattaca ttctcagaaa
     6781 aaatccttgt tttttactca ataaaaattt gtgctccgtt tacaccctga ggccttcatc
     6841 atgttcagaa tacccacacc tgaaccgtcc tttttttatc tatcggaata aaagtaatat
     6901 gagcaattat aaaatttgcc caatagtgta taatgttgtt gaggcattaa aaaattcgct
     6961 tagctaaaag aaagattaaa aacttattca aacttccagc cttcgggata aacagtgcgt
     7021 gtatgctttt ttgttttggc tacagagcga agaatagcaa accttgatgt attccctttt
     7081 agagaacgca gtgttttaat atcttcaagt aacgaaacct gactgtcatt ttgcggtttg
     7141 aacagattca tgcagaaata tgtattcaac attttattgc agtgtttatt ttctccatgc
     7201 aatttaaaag ccgctgaata cagaaatatg ttaagttggg tgaatacctg aaaacgaaag
     7261 gtaaaccact gcaatttttc agttgaagaa gacacctaga aatttactat tcctgcatcg
     7321 gcctgcttat acatgttttc agaaatagaa gtattcaggt cgcgaaaaaa ataaatgcca
     7381 ccccaggcat aaacgacctt actataaagc accgttttat tgcccttagt aacccaaaca
     7441 ttgtaaacag tttttctgtc ttcggccaat tgttcacgtc ttactgaaac attttcagga
     7501 ggctcaggtg ctgtatgaac tactacttct tttggttttt caggtgcagc agtttctgga
     7561 gttttaataa ctaccggttg tggtttagct ggttctgctg caggtggttg gtcagcacct
     7621 tttgtcaagc caattttatt tttaattgca tcattccctg ctgtgggttc aatgtctgcc
     7681 ttttgatctg cgcccttttc aatgtctata ttaccttccg gctcatcact tcctccgcct
     7741 tgtgtaggct ttagtggttg gtcttgtcca cccgtagcgg cagcgctttt attagaattt
     7801 ttcgactcat tcttcgtatc atcattcttg cctttttctt ttacagcaac cggttcttcc
     7861 tgtggtgcag tcttggtttt ttctttctga ggttttactt cttctgcctt aggtttgttg
     7921 gcaacaattg tgctgtcttt ggcaggtttg ggcggattct ttttaaactc tttctctgct
     7981 tttttcaaat catcttctgc cttttgaatt gccgattgaa ttgacttggt ataatcagta
     8041 tcataatcaa actcatcata ctttttcgaa taagtaattt ttgcaacagg ctgattaaac
     8101 actacaatat ttacgccctc atactgcttc atcaacacta ctgtaaaagg ataggactca
     8161 aaaccctgct caactctttc ttcatcggca ccggttgtgt taatatttat ttttttggtg
     8221 atgtatccgg gcttgttaaa agacaagata taatctgccc ctatttcaag atttaaagta
     8281 tatgacgact gccctggaag agtttcaata gactgaccat cacgctgcaa aaccatatag
     8341 gcatcaccca aacttccacc tccgtcaatc ttaaacttgg cattgatggt taatgttttc
     8401 tttgtctgcg cagtggaaac accatgtata aacatgaaga tagtgagaaa aacccctaaa
     8461 ggaactaaat gaattctttt cccaatgcca aacatttgta attttattcc gttaaaaagt
     8521 ttcaatgccg atacaaaatt agttaatagt ttaaacgata gtcctaattt ccaaaataag
     8581 aatgttggtg gtttacgata gaatcaataa agagtttagc aaaagaagaa agttttttct
     8641 gttgctgctg atgcttagtg ttttttcacc cgcactcaga gcacaggaaa acatcaccgt
     8701 acaacaaacg ctcgactata tcaatcagaa atttgcaggc aaatatgtag tttctatcaa
     8761 ctatggcgtt ttaatagcca cttattttga tggcgaaaca aaatacagag aagatcaggc
     8821 agctattaaa gaccttgatg ccactaaggt ttactacaat tcaaaagcag gaatgttgct
     8881 gattaactgc accaacccac aaaaaaaatg tgtcacccgt gagtttctgg gaaaaagact
     8941 tttctacagc cgcattagtt ttccggtttc ttatggacaa aaaaccaacg atggcttggt
     9001 aaaagcattt caacatttaa tacatttgat taatgataaa aattatgaaa attcagaacc
     9061 atttgaataa tgtaaaaata ttgttcattg tattgttatc tgccttttgt gtacaggcaa
     9121 atgcacaggt tgactctgct gattttaaaa tgcgcattga ctcactgatg aatcagggga
     9181 aaataaaatt taataatggt aaatttcagg aagcattgtt agactacacc tatgtggcaa
     9241 acatggatgt agccaacgta aatgcaattt tacaacaagg attttgtaac actatcttaa
     9301 aaaaatatac tgaagctatt ggcaacttta ctgatgtaat tttttatgaa cccaatcatg
     9361 agtgggctta tgtttcaaga ggcagtgcat ataataaaat tgaaaagtat gataaagcca
     9421 tagtagattt taataaagca ttagagctga aacctgaaga tgcagaagcc tacaacaaca
     9481 gaggctttgc taaaaaaatg cttggtgaca ttaaaggtgc ttgcgaagac tggaacaaat
     9541 caaaaaaatt aggaaacgaa gaagctaaaa tcattcttaa aaataattac tgtaaatgat
     9601 caaggtgcgt gagtttgttt ttttaatact gattggtgca gtggtgcttt tatctgcttt
     9661 caaaatacag caggatggaa aagattacgg gcagtgctta caaaaagtaa ataaaaaatt
     9721 tggtgcagaa acattatcat gtcctgcatc aacaaagcct tataaagttt ggtttgtaaa
     9781 tacgtgtagc gatacccttg ccgttaaatt agccgttcag gaaaaatcaa aacgctggcg
     9841 gacttttaca agaacacaac ttttacccgg agatacaata tcaggctatg cctgcacagg
     9901 cgtaggaaaa tatctttact atacaaagca atacaaagac aactctgtgg atttaccttc
     9961 tgatgaagta attgataata gtaataagga ataaaaattt ttactttttg aattctacaa
    10021 caatcaattc cccataatct tcgagtactt ggttccgttt gcaggatcaa tttcgtttag
    10081 cagattgctg acttttactt tctgatcggg ataggctcct gaataaatat ttacaatctc
    10141 atctgcttta gcataaaaca aagtttgtaa taacaacgaa cccggctttt ctttatgtac
    10201 ggacattaac aaatcaaggc tttcagaaat ttgcgacaat ccatcatctt tcttttttgc
    10261 catcacgtca aggcctttca gatgatactg ataaaacatt tcatggtaag gtttaaaaat
    10321 aggattagtc atattttcag agagccagta gcgatttttt gtgccttcaa aagcacgcca
    10381 tcctgcttcg cgtgcattct gcatattgtt tacaatagtt tgtgctttgg caaaataagg
    10441 cgctcctcca tataatgaaa agctgtcata gtccacacca agaataatgt ttgcataaaa
    10501 agcaagtact gctgtcagat ttggattgct tccattctcg ctgaactcta gaggctgata
    10561 ttcgatatac ttaaactgaa aattatcgtc attaaaattt aaagtcggtg aattatatga
    10621 tgtctgaaaa acaggtcgtc ttgaagttat ctgaatgctt cctttatact catcgtttga
    10681 aacacgctca tttattgtta ttgtaacaga acattctatt ctttcctgat taagatattt
    10741 atcagaagtc catttggtgt tattcataaa ttcaaaaata gactgctgta gtgtttcaaa
    10801 cactttttta tctacgttct gaatctgtgg tgccattaca ttaacctggc aattgagttc
    10861 ttgtgcatgc acagtatgca aactcattat gattattact aaacagaaaa atttcttcat
    10921 tgcactaaag taattatttt ctcaacaatg tcggatgcaa ctgcatcttt cgattttttt
    10981 tcaaatggtg ttgtcaccag gtttttatca atgatggtga tgcggttaaa gtctgaaccg
    11041 aaaacctgat tcgattcttc caacttatta agaacgataa aatcgaggtt tttatttttc
    11101 agttttcgct cggcattctg cttttcatca ttcgtttcta aggcaaagcc taccaaaatc
    11161 tgaccttctt ttttcagctt acccatttct gctgcaatgt cctctgttgg cactaactga
    11221 acacttaacc cctcattatt tttcttcttt attttttgat cagaaatgcc ttcgggtcta
    11281 aaatctgcaa cagcagcagc cataaccatt atgctggcat cgggttgtgc ctgtttacat
    11341 gctttcaaca tttcggccgc agaagttata tgaactatct ttattgactt attcaaattt
    11401 cctaaactaa ctgacgggcc tgagataaga attactgatg cgcctttagc agctaaacag
    11461 tttgcaatgg caacacccat tttccctgag gaatgatttc caataaaccg aaccggatcc
    11521 aatgcttcct gtgttgggcc tgtggtgaca actgcaattt tgtttcttaa aggtaacgat
    11581 ggattaaaat aattgattac ttcctgcaca atatcttcag gctctgtcat gcgaccttta
    11641 cctgtaagtc cgctggctaa ttcgccactc tccggaccga taattttaat attgtttttg
    11701 gcaataatct caaggttttt ttgtgttgaa ggatgtttaa acatatccac atccattgca
    11761 ggtgctacca taactgtatt gcggcaagac agaaaaactg cagtcagtaa attgtcgcaa
    11821 atgccattgg caaactttgc aagtgtgttg gctgatagtg gagctaccag aaaaatatcg
    11881 caccataaac ctaattcaac atggttattc caggtagaat tatcttctga agtaaatgtt
    11941 tgaagtgttt cgttttttga aacggtagaa agaacaagag gtgaaacaaa atctttagca
    12001 gcagcagtaa caattacttt aactgttgct ccttgctgca ccaattgtcg cactaattca
    12061 ggtgttttgt aagctgcaat gcttcctgta acacccagca ggattttttt tccctgcaac
    12121 atgattttaa ttttctgcgc tgtctttgtt ttcttctttt gcaggactac ggaaatagat
    12181 tttatcatct aaaaagtcct gaatggcaac caatgttggc tttggcagac gctcgtaata
    12241 tttagagatt tcaatctgct cacggttttc aaaaacctca tccaaagtat ctgttgaaga
    12301 ggcaaactcg cttagtttat cgtgtaattc ttcgcgggtt tcggcagcaa tatgattggc
    12361 acgttttgca ataatggcaa gactttcata gatgttttca gtcttctttt caaagtcaac
    12421 cacatcgcgg gttactgttg tttgtgctac gtttctgcta ttcattgttt taattcaatt
    12481 tacagtatga taatctttta atttttcttc ggctgccgac aatatacggc tggcttcttt
    12541 tttatattcc gttgcaggaa atatctcaat cagcttattg taatgatcta tagtactctt
    12601 gtaacgctct tcttttttcg attctatgct gttttgggca tagagataag ccgatttaag
    12661 cgacaaaaat aaacattctt cctgatattt ggtagtcggg aagtccttca ccacgttttc
    12721 aaaggcaacg atagccgatt tatactcttc cattttgtag taaagcattg cattatccca
    12781 ggcctttact tcgagtttac ttcttagctc atcaatatac ttattacatt cggcaacacg
    12841 ctggcttgtt ggatatttat tgataaataa ctgaaactgt ctgatggcat cgaggctact
    12901 ctgctgatcg aggcttgaag gcggagagtc caagtaatag caataagcat tataaaacaa
    12961 agcatcttca gctttggcac tcattggaaa tgtttgtgca aaattgttaa agtgataagc
    13021 tgcgctggta taatcaccgg tgtagaaata gcttaaacaa taatagtaat aagagctttc
    13081 agccttatcg gttcctttaa gcacaactat taactcttca aaaagctgta atgcattaaa
    13141 atattttttg ttttcgaaat attttactgc agcatcatac ttcatgttca tatcgccact
    13201 tttacgaatg cgattaaact tactgcatga gcctaaggtc agcagcaatg ccattaaaag
    13261 aaaataaacc gacagacgat ttttcataag tgtgcaaaag taaccaaaac actaatacaa
    13321 tgatgtgttt ttaagaaacc cttttagctg ataatcaatt attaaacaat tattcattta
    13381 acgaatgttt tctgtaattt tagacccttt atttaaaatt cataaaatca aatcaatcta
    13441 tgttaaaacg atttctcttt attatgttgt tgtgtactac aacaatggct atggcgcaaa
    13501 cagactatcg cctgatgttt ttaggtaaag ctgttttgcc ggcagaaaac ctaaactctt
    13561 ttatccagga aaaagtatca gaaaacgatg tttataatgg ttattactac cgaatcatac
    13621 agttttatac tgtacctact caagcagaaa aagctgtaat ggaaactgca ggaattaagc
    13681 tgttggagta cttgccaaag aatgctttta atgcagcaat tccaattagt tttaacaaac
    13741 aattgctctc aggatttaat gtcagaagtg taacaaatca atcttctgaa cagaaacttt
    13801 ctatcagcat gttaggtgat tatcctaaat acgctgttcc ttctgcagga atggttgatg
    13861 tgagacttca gtttcagaaa aacatttcaa tacaacaagc acaacagatt gccgctacct
    13921 atggcaaaat tttaagttca actgaaataa ataatatggt tgaggtgcgc atgcagcaac
    13981 aatctgttgc cactatttca caacagcctt ggttgttttt tatagattta acctcgccac
    14041 catctgttaa agatgatttg gaaggacgta gtctgcaccg cagcaatgtc atcaacaacg
    14101 acttgttgat gggacgtaaa tacaatggaa gcggagtagc cgcagcactt gccgatgatg
    14161 gatttgttgg cccacacatt gattttaccg gaaggattac caattacaca acaacagttg
    14221 gtccttcgca tggtgacatg acaggcggaa ttcttgcagg tgcaggcaat ttaaatcctg
    14281 catatccggg tatggccaat ggagttcaac tgcatgtttt tgatattgga agctacccac
    14341 aaattattga tgcagttgcc aactataaca atcttggaac agtggttgct tcaaccagct
    14401 attcgcaggg atgtaatgaa tatactaccg acacacaatt tggcgatcag actattcatg
    14461 acaatggaca acttgaattt gttttctctg caggaaataa caatggtacc aattgtggct
    14521 atggtgccgg aggtaattgg ggaaatatta caggaggtta caagcaggga aaaaatgtaa
    14581 ttgctgtagc caatcttgat gctttggagg ttattgatcc ttcaagcagt attggtcccg
    14641 cttctgacgg cagagtaaaa ccggatattg ctgccaatgg ccgcgaccaa atgtcaacag
    14701 atgaaaataa tacttatcag gttggtggtg gcacctctgc agcctgcccg ggcattgcag
    14761 gtatctgcac acaacttatt caagcttata agcaaattaa ttctgctacc gatgcaccta
    14821 ctgctttaat taaagcttgc ttgctcaatg gtgctgaaga tatcggaaac cctggccctg
    14881 attacaaata tggttgggga agagtaaatg cactgcgtgc tgttactact attgaagatg
    14941 cacgttattt tactgacagt attaatcaga gtgactcatt gacttattct attaacgttc
    15001 ctgcaggtac ttcgcaaatg cgtgtcatgg tttactggca tgatgtaggt ggctcaccgg
    15061 catcatctac ttatttagtt aacgacattg acatgcaagt aaccgaccca tcaaatgcag
    15121 tatggaatcc atgggttctt gatcctactc ccaatgccgc taatctgaat gcacctgcaa
    15181 caagaggcat tgaccatctg aacaacatgg aacaagttac attagacaac cctgcagcag
    15241 gtaactatgt tgtttcactc aaaggatttg ctattccaca aggaccacag acttattatg
    15301 tagtttatga gttcagaaat gatgacatca ctgttaccta tcctatgggt ggcgaaggtt
    15361 ttgttcccgg tcatcctgaa attatccgtt gggatgcatt gaaaggtctt ggctcatttg
    15421 atgtagaata tactactgac aatggaacta catggaataa tattgccact gtaaatcaga
    15481 atacattaca atattcgtgg aatgtgccta ataatattgc aacaacaggt gatgcccgtg
    15541 ttcgtgttac aagaggaagt gtttctggat tgtctgacac aacatttacc attgttggca
    15601 ttccacaaaa tttaaatgtt atttactcat gtcctgattc aattaaaatt ggatgggcag
    15661 ccgtaagtaa tgctgcttgg tatgaagtga gtatgttagg tacaatgtat atggactctg
    15721 ttgccacaac aaccaacacc gattatattt ttacaaacct tatagcatca aactcttatt
    15781 ggtttagcgt aagggcagtg atggctaacg gcaataaagg gcgcagggct aatgccatta
    15841 aaaaacaact tggcattttc aattgtccaa acattcctcc atctgtgcag tttaatgcaa
    15901 acttcaatgt aggttgtaca agcaaaactt tcacttttac agatcaaagc tctaatgcac
    15961 cgtcaagttg gaattggaca tttaatccta ataccgttac atttgtaggt ggaacttctg
    16021 caacatcaca aaatccacaa gtacagttta atacacccgg cacttacgat gtaactttag
    16081 atgctaccaa tggaattggc acttcaagtt caacacaaac tgcaatgatt aatattcttg
    16141 ctccttcttt accaccaatg acacaagatt ttcaggctac agttttccct ccggcaaact
    16201 ggtctattga ttcatcaaac gctaattata catggttaaa atcagctctc attacaggat
    16261 cagcaggcac ttctacttat gctgcacgat tcaacaatta ctcatataat agttcaggat
    16321 cagaagatgg tttaattagt tatgaagtaa gtttatccaa tgcagcagca gcattactga
    16381 catttgatgt agcgtatgca caatattcaa gtgtttataa tgatggtttg cgagttgaca
    16441 tttcaacaga ctgcggaaat acatgggttc cttcaggtta cttaaaaacc ggagtggctc
    16501 ttgctactac gggtaccaca accagcaact ttgctccaaa tgctgccaat caatggcgca
    16561 atgattctgt ttcattgaat gcataccttg gtcaggatgt ttcgataaaa tttgtaaaca
    16621 tcaacgattt tggcaactct atgtacgttg acaatgtaaa tcttgatgtt actacaggat
    16681 taaatcaatt ggacaataat gttggaacgg taaatatttc tccgaatcca tcagacggga
    16741 aatttattat ttacagtaag caaacatcac atgagtcata tacacttact gtttatgaca
    16801 ttaacggaaa agttatagct gaagatgtat tcccggctaa caacaaaagt catgctgttg
    16861 atatcagcaa tttttccaaa ggtgtttatt ttataaaagt tgaaggagaa aaatctgttc
    16921 agcgtttgaa agttatttcc agataaggat aactcaatga tttataaaac cggttgatac
    16981 tatcagccgg ttttttattt aacactatat tgtactgaaa agccaaaaca caaataatta
    17041 tcaaatctat tcgttaataa aaaaaagcga ttattcattc tgccagactt taatttcatt
    17101 tttttgcgtt taaaatctat caaataaaat ttaagacaat cttttaaaaa taatgaacaa
    17161 aaacaattac tgcgtaataa tggccggagg cataggaagc cgtttttggc caatgagcag
    17221 aaatgctttt ccgaaacaat ttcttgacat tttaggaaca ggacaaacac ttttacaaca
    17281 aacgtttaat cgttttacta aaatttgtcc tatagaaaat atctatattg taaccaatga
    17341 atcgtatatt gatttagtaa agaaaaatct gccacaacta ccggtagaaa atattctggg
    17401 cgagccactc agacgaaata cagcaccatg tgtttcgtat gctgcattta aaattgctgc
    17461 aaaaaatcct gatgccaata tagttgtcgc cccgtcagac catatcatca caaaggaaga
    17521 tgcttttatt gaggccatta acaaaggtct tactttcact gcacagaatg actgcctgct
    17581 taccttgggt attacaccaa gcagaccgga tacaggctat ggctacattc agtttgtaga
    17641 tgaaaaacac aatgaaataa ataaaatatt caaggttaaa acttttactg aaaagccaaa
    17701 tctggaaatg gctcgctttt ttctgcaatc gggtgagttt gtctggaatg caggaatatt
    17761 tttatggaac cttaagtcaa tattaaaagc cttaaaagaa catctgcccg agatgtatca
    17821 tctttttgaa gaaggtaatt ccatttacaa cagcaaaggt gaagatgcat ttattaaaac
    17881 agcctattcg caatgtacca acatcagcat tgactttggt gtaatggaga aagcgaaaaa
    17941 tgtttttgtg atgagtgctg attttggatg gagtgattta ggtacttatg gttcacttta
    18001 tgagcacata acacacgatg ctaataaaaa tgcagtggtt gggaaaaatg tgatgttgta
    18061 cgatactaaa aactgcattg taaatatgcc acatgataaa ctggttgttc tacaaggctt
    18121 gtccgattac attgttgttg aagcagaaaa tattttattg gtttgcagaa agcaggacga
    18181 acaacaaata cgtcagtttg tgaatgatgt aaagagtgct aaaggcgaaa agtttgtttg
    18241 atataatatc tcatttagtt taagcgaaca tcaatccgtc accatcaatt taggttaatt
    18301 tttagcattt atgcctaact ttactcgata aatgatgcga ttctcaaact aaatttaatc
    18361 attcttagct tttttaagaa atttgttgtt actttgcaaa ctatttacaa aacagttata
    18421 ttatagtaag caaatcaagt taactacgga ttaacttctc tttaagaaga gggtaaataa
    18481 ctctacatga acccttaaat ccgtcagata aaaatcccat attatttaat gtcattttca
    18541 aatttaaatc tcattactcc cctattggaa gtcttaaacg agttgaaata tagtaagcca
    18601 actcctgttc aggaacaatc catcccaata gtattagccg gtcgcgacat ttttggctgc
    18661 gcccaaacag gtacaggaaa aacagcagcc tttgccttac ctattcttca acatatttcc
    18721 cttaaaaagc aaaaaccagg aaatcacatc aaagctttgg tacttgcgcc aactcgtgaa
    18781 cttgccttac aaatcggtca aagtttttcc gaatatggcc gtaaactccc ttacagaaat
    18841 gttgtacttt ttggaggagt atcgcaagta aaccaaacaa atgccttaaa aaatggagtt
    18901 gatatattaa ttgcaactcc cggccgtttg ctcgatttaa tgaatcaggg gttcatagat
    18961 ttaaaacata tagagtattt tgttttagat gaagcagaca gaatgttaga catgggattt
    19021 atcaatgata taaaaaagat aattgtaaag cttcctcaac aaaagcaaac tttgtttttc
    19081 tctgcaactg cggcaccgga aatcatgaaa ttagccaaca cattattaaa aaatccggct
    19141 aaggtcaatg ttactccaat ttcaacaccg gctgaacagg taaaacagtt agtatatcat
    19201 gtaaaaaaag aaaataaacg ctccttgtta cgtcatgtat tagagtccaa taaaattaat
    19261 catgccttgg tttttacaag aacaaaacgt ggtgcagaca gggtagtaaa agatttaaat
    19321 aaaaacggta taaaagcaga ggctattcat ggcgacaagt ctcaaggtgc aagagaacgg
    19381 gctttgaatg gtttcaaaaa aagatcaata caaattttag tagcaacaga tattgcttct
    19441 cgtggtattg atgtggacag actgacccat gttattaact ttgaaattcc tgaacaggcc
    19501 gaaacttatg ttcatcgtat tggccgtaca ggtcgtgccg gtgaatcggg tatagcttta
    19561 tccttttgca cagatgatga aaagccatat ctgaatgata tcaataaatt aatcaaacaa
    19621 aacattgatg ttgtaaaaac acatccatat aaataattca aactaaagta aaattaaatt
    19681 caacaaacaa tgcaaaatgg tacagtaaag ttctttaatg aaaccaaagg ttttggcttc
    19741 attaaagcag aaaacggaga agaaattttt gttcactctt caggtttaaa agaacaaatt
    19801 cgcgaaaacg acaatgttgt ttacgaagta caacaaggaa aaaaaggatt aaatgcagta
    19861 aatgtaagat tagcctaaaa ttccatattg taaaaaagta aaaaaggcta tctcattaaa
    19921 tgagatagcc ttttcttatg cagcagaacc tgatataata ctgataaaag agtaccctca
    19981 gcgttcgtta caagataata tttcgtcttg actctttata aaatttattt tactgtttgg
    20041 acacgcctta ttttgaacat catattcctt tcaattcgca ataaataata taaatgatct
    20101 tattaatgtg tgtatttacg aaaccagctt tttattttca tctgaataac gccaaagaaa
    20161 gcttctttaa aaataccgct gctcattttt gatttacctt ctgttcgatc ggtaaaaatt
    20221 ataggaactt cgataattcg gaagccgctg caccaggcag caaatttcat ttctatctga
    20281 aatgcatacc ccataaattt aatctttgac aaatccagtg tttcaagcac tttacgcctg
    20341 taacatttaa atcctgctgt ggcatcacga acaggcatgc gtgtaatcaa tctaacataa
    20401 acactggcat aatacgacat gataactcta cctatcggcc agtttacaac attcacacca
    20461 ttgatatatc ttgaacctac tgctacatcg gcaccaccat ttttacaggc atcgagcaat
    20521 cgttgcaagt cggcaggatt gtgagagaaa tcggcatcca tttcaaaaat atactgatag
    20581 ttgcgctcta atgcccactt gaaaccatgt atataagcag tgcctaaacc catctttccc
    20641 tgccgttgaa ccaaatgcaa tgactccgga aactgaggca taagttgttt caccaaatcg
    20701 gcagtaccgt ctgatgaatt gtcatcaata accaatacat gaaaagtatt caacagatta
    20761 aaaacagctt taataatctt ctgaatattt tctttttcgt tatatgtcgg tatgataact
    20821 aatgattcga tcacacaatt ttatttgagt tcaaaactat tattctctga aatgagtaat
    20881 tctaacaata tgttaattga taaaactatt catcaattat ttacctgtac tgccaaaacc
    20941 accagcacca cgattggttt ctgacaactc cagtgtttct ttccacacta cttgttgatg
    21001 tgctgccaca accatttgcg ctattctttc gccattattt attgtaaatg ccaccgatga
    21061 cagatttacc aaaataactt ttatctctcc acgatagtcg gcatcaatag tacccggagt
    21121 gtttaatacg gtaacaccat gctttgctgc aagtccactt cttgggcgta tttgtgcttc
    21181 aaaaccttga ggtaattcaa tataaattcc tgttggtatt aaagcacgtt ctaatggctt
    21241 aagttctacg gcttctttca gatcagctct aaggtccatg cctgcagcac tgttgctttc
    21301 ataagcagga agcggatgcg atgatttatt tataatcttt acttctgttt tgttttgcat
    21361 ttttaattct ttcgctattt gcaaaaatat tgattcttgt caaatataca ctcagtttct
    21421 tcttccaatt gtattctgct gcgattctac tttttgtttt tttaccagca gttctttatt
    21481 cattctgaat acaacaaacg gaaaaacaaa gagtaaaatt atatttacaa tccatttaac
    21541 agaaccaggt aaatcaagtt gatgttgcag ataggcagaa agtgaaaaca gcattagaga
    21601 tagcaaaaca taaaacacat cgttcttaac tttataggga ataggataat gtttctgtcc
    21661 ggtgatgaaa gatacaatca tcattgcaaa ataagtaatg aaagttgccc atgctgcacc
    21721 catatatcca aacgaaggaa taagaacaaa caatagaata atagtcagca ctgctccgaa
    21781 aattgaaaaa taggcgccat agcgggtttt ttctgtgagt ttataccaca tacttaaatt
    21841 gtaaaataca ccaagacaaa gattcgccaa caagagcaca ggaaccacat caatacccga
    21901 cctgaattgt tcaccaataa aaagtttaat gacgtcaata aataacatta cacctaaaaa
    21961 aataaatgag catgtgataa caaaccatgt cattacttta gcgtaagttt cttttttatc
    22021 ttctttatca tgctgcgcaa agaaaaatgg ctcggcagca tatcgaaatg tctgcacaaa
    22081 caatgtcatg atgatgctaa gtttgtaaca agcgctgtaa attcctaaat cgcgcaatgc
    22141 atgattggca tccggagcta agtatttata aacagcacgg tcgaatgttt cattaaccat
    22201 acctgcaaat cctgcaataa gcaacggaaa tgcataataa atcattcgtt ttattaaagc
    22261 actgtcgagg tgccaggtta aattactgaa ttgccggtag agtaataaca tggtaaaacc
    22321 actggctata acgtttgaaa taaatacata tcctacaccc actgttggag aataaaaagt
    22381 gacagcaagt tgcgataaca aaccatcatt tcctttaatc catatcggac agccaataag
    22441 aaaaaataaa ttcaaaccta tatttatcac tattcctgca atctttaata aagcaaactg
    22501 taatgctttg ccttgttgtc gtagtcttgc aaatgggatg gcagtgatag catcaaaacc
    22561 aataatcaat gcaagccagg tgatgtagtt tccttttccg ggatactcaa tagttgcagc
    22621 caatggcgat gagaaaaata tcatcagcaa cacca
//
LOCUS       DAOOOL010000027        22613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_174_length_22613_cov_110.681443, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000027 DAOOOL010000000
VERSION     DAOOOL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22613
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174_length_22613_cov_110.681443"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..3434
                     /gene="secDF"
                     /locus_tag="PKU82_03830"
     CDS             417..3434
                     /gene="secDF"
                     /locus_tag="PKU82_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOZ82035.1"
                     /translation="MQSKGAIKFLAILVSIICLFQLSFTIVTNRIESKAHEATNGNPE
                     AYKAYIDSISQLTAYNLGFKKYTYQECKEREINLGLDLKGGMNVTMEVSVTDLIRNMA
                     NNPNDPMLVKALDDAHQAQRTSQKGLVQLFGEAFERNNPGAKLAALYSTMDLQNRVNF
                     NSTNAEVIKVIQSEADAAISRTYNILRTRIDKFGVTQPNIQQLGGGRILIELPGVKEP
                     DRVRKLLQGTAQLEFWETYDNAEVFPQLEAVNKKLAALETPAPTDSTAKSDTTVAATG
                     DSLTAAADTGAMKKPALLSKLESDTAAAKDTSSSAQFAKFSKENPLFALLTPAIFTNA
                     QNQSEYRKGPVVGYAAIKDTGKLREILERPEIKNILPRDLRLLWTAKPPTKESKFLEL
                     VAIKVKSRDGKAPMDGSVITDARQDFGQFNSSPEISMSMNPEGAKTWKRLTGENVGKS
                     IAIVLDNYVYSFPNVQGEIAGGRSSITGNFEISEAQDLANILKAGKLPAPARIVEEAI
                     VGPSLGEEAIQNSWMSFAGAFVVILLFMGFYYMRAGWVANIALFANIFFIMGVLASLG
                     AVLTLPGIAGIVLTIGLSVDANILIFERVREELKAGKGIRLAVSDGFKHAMPSILDSN
                     ITLLILGIVLYIFGSGPVQGFATTLVIGVLTSLFSAVLLSRLIFEGMLDKNKPISFGN
                     EFSNNIYKNIHFNFVDNRKKYYILSSIVIIAGIVSFAIKGLNYGVDFEGGRTYTIQMD
                     KSISTEDIRMALEPQLVEAPEVKSVGTTDRFKITTAYLIHDTASNVENVIVDKLYKGL
                     TPLYGSAPDFETFKTKNIISSQKVGPTIADDIKQSAVWAVLISCALMFVYIFIRFKKW
                     QYGLGAVVALFHDVLIVLSFFTLLSGVIPVALEIDQHFIAAILTVMGYTMTESVVVFD
                     RIREYLGHHHTADDKHVINEALNATLSRTINTTLTVFFVLIVIFIFGGEVIRGFIFAL
                     LIGRIIGTYSSLCISTPIVIDLGGNAHEGVAKK"
     gene            complement(3728..4354)
                     /locus_tag="PKU82_03835"
     CDS             complement(3728..4354)
                     /locus_tag="PKU82_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82036.1"
                     /translation="MKKHKQLSNLSTFAFIIAFLLTSCSSIIGTNKLKQVDEKTILHY
                     AKKYNINSTENYELDTAYFTFLKSFDTKLFKQQIKNHYPPLQALYYNKTGQLQSFQIN
                     CYAGGFPNLKWNKNGIIKTFPPKQQAPLDTIISLKTQLKFLKPLSNTEKSTTNDYYYI
                     VIIYWSKFMGRQSKRLIGFVQDNVKLATDKRVKIIYVNTDNFFADNLK"
     gene            complement(4397..5626)
                     /locus_tag="PKU82_03840"
     CDS             complement(4397..5626)
                     /locus_tag="PKU82_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOZ82037.1"
                     /translation="MNLIIKIASRYLIGKKSHNIVNLVSLVSIAGVFTGTMALIVVLS
                     VFNGFGNLVISLYDTFDPDIKITVIKGKTFNSDALNLEKIRAIDGIKYAAFTLEENCL
                     ARYGEKQDIVTLKGLDNELMKKTGLNKAVVIGLPILENGERNFAIVGSGVAYALSVNA
                     DTGNEPLMLYLPDKNITSPGDFENALRQMNISIGGIFAIQQDFDSKYILAPMSFVQEF
                     IAAEGKASAMEIMLKPGADETTVIQKLKEISGHDFTVKNRAQQHEFLYKILSSEKWAV
                     FLILSFIMVIGIFNILGTLTMLVIEKKKDIIILKNLGAGFTMVKKIFFMEGLLITFVG
                     VVGGLLAGGIICFLQQHFHLIKLGNADSFVVEAYPVQMQVTDFVSVFLVVFCIGALAS
                     YFIAAHLVKKQYAETGK"
     gene            5770..6897
                     /locus_tag="PKU82_03845"
     CDS             5770..6897
                     /locus_tag="PKU82_03845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOZ82038.1"
                     /translation="MKKLHMVDLISQYQKIKPEVDKAISDTVTSSAYINGPEVKAFQA
                     ELEKYLNVGHVINCANGTDALQIAMMALNLQPGDEVITASFTYVATAEVIALLKLKPV
                     LVEVIPGTFSIDPVAIEKAITPKTKAIVPVHLFGQCADMEPIMKIAAKHNLYVIEDVA
                     QAIGADYTFSDGRKQKAGTIGTIGCTSFFPSKNLGCMGDGGAIFTNDAELAKKIRMIA
                     NHGQSVQYVHDEIGVNSRLDSIQAAILRVKLKHLDSYAAARQKAAAYYDNAFRGSTKI
                     KTPERSKNSTHVFHQYTLVLDGVNRDELRHYLAEKEIPAMIYYPIPLHMQKAYKDDRY
                     KEGNFPVTEKLCANVLSLPMHTELDEEQLEYITRAVLEFCK"
     gene            6928..8241
                     /locus_tag="PKU82_03850"
     CDS             6928..8241
                     /locus_tag="PKU82_03850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOZ82039.1"
                     /translation="MNIGVVGTGYVGLVTGTCFAEMGNHVVCVDIDQAKVDSMKQGKI
                     PIYEPHLDVLFERNIRQNRLSFTTQLTDAVKHSSIIFLALPTPPGEDGSADLSYVLGV
                     ADQLGKMLDGYKIIINKSTVPVGTAEAVRDAIAKNYKGEFDVISNPEFLREGFAVDDF
                     MKPDRVVIGTRSEKAKKIMGELYAPFLRQGNPIYFMDERSAELTKYAANSFLATKITF
                     MNEVANLCEKVGANVDAVRIGIGSDDRIGKRFLFAGIGYGGSCFPKDVQALEHTSGKY
                     GYDFSILQSVMSVNNKQKMLLAEKALEYYGGNVKGKRFALWGLAFKPDTDDIREAPSL
                     YIIDMLTKAGATIRAFDPEAMNNVKKLIGDKIEFANDEYHALEGADALLIATEWSAFR
                     TPDFDKMHSALKSKVIFDGRNLYEPQKMQEAGYYYNSIGRQTVKP"
     gene            8238..9209
                     /locus_tag="PKU82_03855"
     CDS             8238..9209
                     /locus_tag="PKU82_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ82040.1"
                     /translation="MTKRILITGAAGFLGSHLCDRFIQEGYQVVGMDNLITGDLNNIA
                     HLFKLREFDFYHHDVSKFVHVPGKIDYILHFASPASPIDYLKIPIQTLKVGSLGTHNL
                     LGFALAKKARILVASTSEVYGDPLVHPQNEDYWGNVNPIGPRGVYDEAKRFQEAITMA
                     YHTYHKLETRIVRIFNTYGPRMRLNDGRALPAFVGQALRGEDLTVFGDGSQSRSFCYV
                     DDLVEGIFRLLHCDYAQPVNIGNPVEITIKQFAEEIIKLTGTTQKIIYKPLPQDDPKQ
                     RQPDITRAREILGWEPKVSREDGLKITYEYFKSLPPEELKRSAHRFA"
     gene            complement(9952..10911)
                     /locus_tag="PKU82_03860"
     CDS             complement(9952..10911)
                     /locus_tag="PKU82_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOZ82041.1"
                     /translation="MSNTKSNNIKLGFFVMSGLIVLVITLYMIGSKRSVFSKTIEISA
                     VFHNVNGLMPGHNVRYGGIDIGTVQKIIFESDSAITVKMIIEKKMAPYIKKNAIASIG
                     TDGLMGNKLVNINSLPAPSSPIEKGDVLLSLRPVESDEMIRTLNKTNDNLAAITSDLK
                     GLVQRFNKNNNLITLISDSAVVENLRQSIQSINSATNNIDNFTKDINNITKDVQNGKG
                     VLGKLIADSISENQLAILLTNLQQFSDSLNEATNNINQFSGLLVNGQGTVQALLTDTI
                     MKNDFNETLNQLKKSSFLLEEDLKALQKNFLFRKYFRQKEKRK"
     gene            complement(10908..11657)
                     /locus_tag="PKU82_03865"
     CDS             complement(10908..11657)
                     /locus_tag="PKU82_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ82042.1"
                     /translation="MMDNTNIILKIEGLCKAFGSNVVLNNFNLELIKGENIVVLGKSG
                     SGKSVLIKCIVGLLKPDKGNIHVFDTNMLELDQESLDKIRIKIGFLFQNSALYDSMTV
                     RENLEFPMRRHWISLTSSEVKNLVEESLDNVGLLHAINMMPSELSGGMRKRIGIARTL
                     IMKPKLILYDEPTTGLDPITSREIVQLMLDLQKKYHTSSVIISHDMSVAKLTANKIIV
                     LLDGRNYAEGTYDELKKSSDKNINQFFEFAS"
     gene            complement(11657..12427)
                     /locus_tag="PKU82_03870"
     CDS             complement(11657..12427)
                     /locus_tag="PKU82_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ82043.1"
                     /translation="MKLPKGISDLLHETGQLSSFVVRFFKEGFKPRYEWEELFKQAFI
                     NGYKSLPLVGITAFIMGLVLTVQSRPTLAEFGATSWLPAMIAISIVREIGPVITALIC
                     AGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKFLVVTRVLSIAFMLPILVILADLIA
                     IYACYIGVNFKDVVSWKLFYNKVFNSLEYSDFIPALVKSFFFGLTIGLVGCFKGYNSQ
                     KGTEGVGHAANSAVVVASLIIFILDLIAVQITDLLGLN"
     gene            12578..14140
                     /locus_tag="PKU82_03875"
     CDS             12578..14140
                     /locus_tag="PKU82_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOZ82044.1"
                     /translation="MADKEITQPIQTDSGIEVKQVYTSCKPMDELPAKFPFTRGVQAD
                     MYRSKLWTMRQYAGFSTAEESNKRYHYLLKQGTTGLSVAFDLPTQIGYDSDHELSEGE
                     VGKSGVAIDSLRDMEVLFDGIQLQNITTSMTINSTAAVLLSMYIALAKKQGADLSKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFDFCSREVPKWNTISVSGYHIREAGST
                     AVQELAFTLADGKAYLKAAIDAGLDINVFAKRISFFFNAHNNFFEEIAKFRAARRMWA
                     SITKELGATDPKAMMLRFHTQTGGSTLTAQQPQNNIMRVTMQALSAVLGGTQSLHTNG
                     FDEALSLPTENAARIALRTQQVIAYESGVTQTVDPLGGSFYIESLTDEVEAKAWDYIR
                     KIDDMGGAVAAIEAGYIQNEIAESSYNYQNAIQTGEKVIVGVNKFQVEEPVFDDIFSV
                     DDTIRKVQVQKLAQLRTERNNEKVNQALSNLESAAKDNANLMPYIIQCVEHYTTLGEI
                     SDKLRKVFGEYR"
     gene            14189..14929
                     /locus_tag="PKU82_03880"
     CDS             14189..14929
                     /locus_tag="PKU82_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82045.1"
                     /translation="MKKRILITLAVSTLFFTACKKDKDSKPAESELSFHLHTLVGNTA
                     ANYTDIFTDNTGRKFNIADCRYYISNIVLIKDDGTEFPLTDKALLASPSQKEYSLGNV
                     PVGKYKGFRFLMGLDSVTNHDDPAGYAASNPLSYQNPTIHWGWNNGYIFMEFVGKFDS
                     TVAATGNPVADYFYHIGFDDLKRTIDFSNSPFEITTDAGLEIGILFDLKKVLNNVDLR
                     TEFGTHTMDNMPLAVKIANNWQGAYSIE"
     gene            14963..15727
                     /locus_tag="PKU82_03885"
     CDS             14963..15727
                     /locus_tag="PKU82_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82046.1"
                     /translation="MKSIKSVLCLAVVGFIFSFSSCKKDDSDANNIPNTDTIGELSFN
                     FRQFVGNDRIVYSQVYNDTSGRPFKITNLRYYISNIVLIKANGLELPLSGKVLLVDNE
                     KEQYTLGEIPVGDYSGLRFMVGIDSATNHLDPALYDASNPLSYQPVSMHWGWNFGYIF
                     MKVEGNVDTDSVPTGNLNGIISYDIGSDPLVRVINFNHIFSVHGNINTVNFKFNILKM
                     FSGIDLATESITHTNPGRDLADRIASNWLLAFTIEP"
     gene            15724..16734
                     /locus_tag="PKU82_03890"
     CDS             15724..16734
                     /locus_tag="PKU82_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ82047.1"
                     /translation="MIKIQVSKIFILSVIVFWIVSIVSCRKDDAIGTGNEVLELEVPL
                     GFPYPSIPEDNQPTKNRIALGKILFFDPILSRDSTISCSSCHHADKKFTDGLKVAEGI
                     EGRKTLRNSMTILNTAYQPNMFWDGGSPTLETQVSAPIEAHEEMDFNVMKVVERLNNH
                     PLYPSLFRKAYNSGPSAFTLTRAIANFERTLFTGKSKYDAFQYEKKSDALSASEKRGM
                     DIFFGERAECFHCHGGFNFTDFSFKNNGLYEVYADSGRARITTLHDDVGEFKVPSLRN
                     VALTAPYMHDGSVATLEDVVEHYNSGGKPNFNKSFLIRPLNLTQQEKEDLVNFMKALS
                     DQ"
     gene            16757..17170
                     /locus_tag="PKU82_03895"
     CDS             16757..17170
                     /locus_tag="PKU82_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82048.1"
                     /translation="MKNIALYILTFGMTVFLASCHKDDSCNAGTGGNNSIKATLLHHT
                     KVISPKVGYPDTLFIKYDTKDFPGENASDFDAYFVNSDTAISTIEITGLKCGDYYLFG
                     TGWDADISQRVKGGIPVTIAEDAGVLTKNVPVTED"
     gene            complement(17173..18798)
                     /locus_tag="PKU82_03900"
     CDS             complement(17173..18798)
                     /locus_tag="PKU82_03900"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOZ82049.1"
                     /translation="MHIALAQLNFHVGNFENNTSKIIEAIKQAEKSGAELIVFPELAI
                     TAYPPLDFLEFDDFINRCYNSIEKIAAHSQNIAVIVGCPSRNAVPEGKNLFNTAWFLH
                     KGKVQALVNKALLPTYDIFDEYRYFEPGRKFECIEFNGEKIALTICEDLWNVEDDPLY
                     IHSPMEELIKEKPSLIINIAASPFDYKHAHQRKAILQRNVNQYKIPLLYVNHVGAQTE
                     IIFDGGSLAYDASGKLVHEGPYFKEEIIYLNTKNTTLVVEKQTEKYELIYQALLMGIR
                     DYFSKMGFKKAILGLSGGVDSAIVTVLAADALGAENILPVMLPSAFSSGHSISDSEQL
                     CQNLNIKVDKMEINPLYETFLKTLKPLFGEKPFDVTEENIQARIRGTLLMALSNKFGP
                     ILLNTSNKSELAVGYGTLYGDMCGGLSVIGDLYKTEVYELCRYINRKKEIIPENILSK
                     APSAELRPGQKDSDSLPPYDILDKILFEYIELRKGPKEIIKGGYDETLVNRILSMVNR
                     NEYKRKQLSPVLRISSKAFGLGRRMPIVGKYLS"
     gene            complement(18973..19563)
                     /locus_tag="PKU82_03905"
     CDS             complement(18973..19563)
                     /locus_tag="PKU82_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ82050.1"
                     /translation="MRAKVSLIAIAFVAAFQFAKAQDNKFNFGLKISPTVAWFKASDN
                     LDNDGSKIGFAYGLIADINFTNNYAFETGIDVTYRGGKLKYMDIYKMKTVLQYIELPL
                     TLKLKTNEIGYMTYFGKVGFTTGINIRTDGDFKKSDINPLNMALVAGIGTQYSLGGKT
                     ALLFGITFNNGFLDVVKEKHSKATSNFLAVDLGVMF"
     gene            19597..20616
                     /locus_tag="PKU82_03910"
     CDS             19597..20616
                     /locus_tag="PKU82_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82051.1"
                     /translation="MKFSTVRQTVYLLLSTVLMLGFSCQNDPHGDKHTKDTMTTHLKF
                     DEVLFSADSINPQHVVQWKQQYPYFFNLFVNRIIHLPPTDSAALALNLQKFVGDKDVK
                     DIHARVEKVFSAADKDKLFSEIEGFLNNYKKEFGIQPVKHLVTFLSAFNYAVITADST
                     IGIGLDMYLGSDCEFYPSIGIPQYAYQRFSKDYILSDVIKGWYQSEHDVDAEKNDFLS
                     RMIYYGKQLYFTSVMAPQVQDTILTGYSKKQLEWCSNNSKQMWAFFIENKLFYSTREM
                     EYMKYIADGHATQGFPEGAPARTAQWLGWKIVSTYMKNNNVTLKELLAEKDAQRILEK
                     SGYKP"
     gene            20647..20976
                     /gene="gldC"
                     /locus_tag="PKU82_03915"
     CDS             20647..20976
                     /gene="gldC"
                     /locus_tag="PKU82_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HOZ82052.1"
                     /translation="MKKSEIKFTVSLDDNNKPVALHWNAEDSGMQGDKECKAMMLALF
                     DKKDQTTMRIDLWTQEMMVEEMQQFFFEMYMSMADTFQNATNNKDMADKMRNFARQFG
                     KEVSLIK"
     gene            complement(21002..21409)
                     /locus_tag="PKU82_03920"
     CDS             complement(21002..21409)
                     /locus_tag="PKU82_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ82053.1"
                     /translation="MTTHILYTEFILYVNNLQASTVFYSKLFRKIPDLNASGMTEFIL
                     SPNCKLGLMPDNGIAKIISEKMPHPNKGTGIPRCELYFYVDDIEAEYANALKCGAKLI
                     SPIADRDWGDKVCYFADNDGHIIAFAVKQNSAK"
     gene            21461..21637
                     /locus_tag="PKU82_03925"
     CDS             21461..21637
                     /locus_tag="PKU82_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOZ82054.1"
                     /translation="MKLQRYLETYDIDNIKDAKQFLKTVVDLYNSERPHMSISNFTPN
                     SIHHSKTTIKTERL"
     gene            21893..>22613
                     /locus_tag="PKU82_03930"
     CDS             21893..>22613
                     /locus_tag="PKU82_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOZ82055.1"
                     /translation="MDFDALLKLLQDWTNRVPLIILGSGASVPFHLPSMWTLGDHLKK
                     SISFADADDKKQFEEFKTTFDMVGDLETTLLQLQLRPNVLKEIIFETWRLVNKHDLEA
                     YEKILNKKVEFPLADITRHLISAAQRKLSIVTTNYDRLAEYAASLSHAFICTGYTQNY
                     FGHFSKLIHQQDLTKLNGFSGQVNIWKVHGSLDWFKSKEEVDFHLPLRQTIPTDFKPS
                     IVTPGLSKYLETQLEPFRTIFT"
BASE COUNT         7313 a         4056 c         4195 g         7049 t
ORIGIN      
        1 tacccgcgaa gttgcacttc gtgggttttt gtgcaaagaa gttttacttc tgaaaccata
       61 ccgtttcctt tattttttat tgtgtaaata taatttaatg ttcactttgt aaagcacagc
      121 attatgacac actactcacg aagtgcaact tcgcaggtac ggggggataa agttcatgta
      181 aatgaatagg agtaaacaat aaaaaagtaa aaagaaaagt attacaatgc atttaaaatg
      241 ctctacatgt gctaatattc tgaaacagag cacttaatta attaactatt ttgtggtcag
      301 acctagacaa agaaataggt agtgtattga aaaacaacac tgtaggttag tgcttaggtt
      361 tgttaaaaac ctttatattt gccgctctta aaaaaatacc gaaaaataaa ttattcatgc
      421 aaagtaaagg cgcaattaaa tttttagcca ttctggtatc catcatctgt ctgtttcagt
      481 tgtcgtttac catcgtaaca aatcgtattg aaagcaaggc acatgaggca actaatggca
      541 accccgaagc atacaaagca tacattgact ctatcagtca gttaacggct tataatcttg
      601 gctttaagaa atacacctat caggaatgta aggaacgtga aatcaacctg ggtcttgacc
      661 taaaaggtgg tatgaacgta acgatggaag ttagcgttac cgatttgatt cgtaacatgg
      721 ccaacaatcc taacgatcca atgttagtga aagctttaga cgatgcacat caggcacaac
      781 gtacaagcca gaaaggttta gtacagttgt ttggtgaagc ctttgaacgt aacaatccgg
      841 gagcaaaact ggctgcactt tacagcacca tggacttgca gaacagagta aatttcaact
      901 ctaccaatgc cgaagtaatt aaggttatac agtcagaagc agatgcagct atcagccgca
      961 catacaacat tcttcgtaca cgtattgata aattcggtgt aacacaacca aacattcagc
     1021 aattaggcgg aggacgtatt ttaattgagc ttcccggagt aaaagagccg gatcgtgtgc
     1081 gtaaactgtt gcaaggaact gcacaattag agttctggga aacgtatgat aatgctgaag
     1141 tgtttccgca attagaagca gtaaataaaa aacttgctgc acttgaaaca cctgcaccaa
     1201 cagactctac tgcaaaatca gacacaacag ttgcagctac cggtgattct ttaactgctg
     1261 ctgcagatac aggagcaatg aaaaagccgg cattactcag caagttagag agtgatactg
     1321 ctgctgcaaa agacacttct tccagtgctc agtttgcaaa attttctaaa gaaaatccac
     1381 tctttgcatt gctgacacct gcgatcttta ccaatgcaca aaatcagagc gaatatcgca
     1441 aaggccctgt agtaggttat gctgctatta aagatacagg taagctgcgt gaaattttag
     1501 agcgtcctga aataaaaaat attcttccac gcgatttgcg tttgttatgg acagcaaaac
     1561 caccaacaaa agaatctaaa tttttagagt tagtagctat taaagtaaaa agccgcgatg
     1621 gaaaagcacc aatggatggt tctgtaatta cagatgcacg tcaggacttt ggtcagttta
     1681 acagctcgcc agaaatttct atgtcaatga atcctgaagg agctaaaaca tggaaaagac
     1741 ttacaggaga aaacgtagga aaaagtattg ctattgttct cgataattat gtttattctt
     1801 tccctaatgt acagggcgaa attgcaggag gccgttcatc cattacaggt aacttcgaaa
     1861 tttctgaagc acaggatttg gctaacattc ttaaggcagg taaacttcct gctcctgcac
     1921 gaattgttga agaagctatt gttggtcctt cattaggtga agaagcaatt caaaacagtt
     1981 ggatgtcgtt tgcaggtgca tttgttgtaa tcctgttgtt catgggattc tattatatgc
     2041 gtgcaggttg ggtagctaac atcgcattgt ttgccaacat attctttatc atgggtgtgc
     2101 ttgcatcttt aggtgctgta cttacgcttc cgggtattgc aggtatcgtc ctcaccatag
     2161 gtctttctgt ggatgccaac atccttattt tcgagcgtgt gagagaagag ttaaaagcag
     2221 gcaagggaat aagacttgct gtttctgatg ggttcaagca tgccatgcct tctatccttg
     2281 actcaaacat tactttgctc attctcggta tcgtacttta tattttcggt tccggtccgg
     2341 tacagggttt tgcaactaca ttggtaattg gagtactcac atcactgttt tctgctgtat
     2401 tgctttcaag actcattttt gaaggtatgt tggataagaa caaaccaatt tctttcggta
     2461 atgaattctc caataacatt tacaaaaaca ttcacttcaa ctttgttgac aacagaaaga
     2521 agtattacat cctgtcttca attgtaatta ttgcaggtat tgtttctttc gcaataaaag
     2581 ggttgaatta cggtgttgac tttgaaggtg gacgcaccta caccattcag atggataaga
     2641 gtatctctac cgaagatatt cgtatggcat tggagcctca gttggttgaa gcacccgaag
     2701 taaaatcagt aggtacaaca gatcgtttca aaattactac tgcctatctt attcacgaca
     2761 cggcaagtaa tgtagaaaat gttatcgttg ataaacttta taaaggactg acaccacttt
     2821 atggttctgc tcctgatttt gagactttca aaacaaagaa tatcatttca tcgcaaaaag
     2881 taggacctac tattgcagat gatattaaac aaagcgcagt gtgggctgta ttgatttcat
     2941 gtgcgctgat gttcgtttac atctttatcc gattcaaaaa atggcagtat ggacttggtg
     3001 ccgtagttgc cttattccat gacgtattaa ttgtattgtc attctttaca ttactgtcag
     3061 gagttattcc cgtagcactt gaaatagatc agcactttat tgcagctatc ttaacagtta
     3121 tgggctatac catgacagaa tcggttgtag tgttcgaccg tattcgtgaa tatctaggac
     3181 atcatcatac tgccgatgat aaacatgtga ttaacgaagc attaaatgcc acattaagcc
     3241 gaaccatcaa cactacatta actgtattct tcgtgttgat tgtaatcttt attttcggtg
     3301 gcgaggtaat tcgtggtttc atttttgcac tcctcatagg acgtatcatc ggaacgtact
     3361 cttcgctctg tatttcaaca ccaatagtaa ttgaccttgg tggaaatgca catgaaggtg
     3421 tagcgaagaa ataatttctt aaaatatttt atttggaaaa ggctgtctca aaaaggcagc
     3481 cttttttgtg tttttaaatt tcaatgacct ttaaaaacta aattttatta ttggaaaatt
     3541 gagaattaac taagccgttc attttcttgt tgtacttgta attcctcctg aaaaaatttg
     3601 gtttattaca tctggcattt taagatatgg cgacaacttt gtacaaaaac tgaattgtat
     3661 ttcaaaagta aaatcttatg aataagcttt aataaaacat attgcacaca tgctgtcatg
     3721 ttatttatta ttttaaattg tctgcaaaaa aattgtcggt gtttacataa ataattttta
     3781 ctcttttatc tgtagcaagt tttacattgt cctgaacaaa tcctattaat cgtttgcttt
     3841 gtctgcccat gaatttactc caataaataa taacaatata gtaataatca tttgtggttg
     3901 atttttctgt atttgacagg ggttttaaaa atttcaattg tgtttttaac gaaataatag
     3961 tgtctaatgg tgcctgttgt tttggaggaa atgttttaat aattccattt ttattccatt
     4021 ttagatttgg aaatccacct gcataacagt ttatttgaaa tgattgaagt tgtccggtct
     4081 tgttgtagta aagtgcctga agaggtgggt aatggttttt gatttgttgt ttgaaaagct
     4141 tcgtgtcaaa tgactttaga aaagtaaaat atgcagtatc taattcgtaa ttttctgttg
     4201 aattaatatt gtactttttt gcataatgta gaattgtttt ttcgtcaact tgtttgagtt
     4261 tattcgttcc aattattgat gaacaacttg tcaacaagaa agcaataata aaagcaaagg
     4321 ttgacagatt tgatagttgc ttatgttttt tcattagtat gttctatcgt tgtttaagtc
     4381 atctaaaaat tgtcaattac ttccctgttt ctgcatattg cttttttact aaatgtgctg
     4441 caataaaata ggaagcaaga gcaccgatac aaaaaactac taaaaacaca cttacaaaat
     4501 cggttacctg catttgaacc ggataggctt ctacaacaaa agaatcggca ttgcctaatt
     4561 tgataagatg aaaatgttgt tgcagaaaac aaatgatgcc acctgccaac aagccaccaa
     4621 caacacctac aaaagtgatt aaaagtccct ccataaaaaa tattttttta accattgtaa
     4681 aaccggcacc caagttttta agaatgataa tgtctttctt tttctctata acaagcatag
     4741 ttagtgtgcc cagaatatta aagatgccaa ttaccatgat gaaagacaga atgagaaaca
     4801 cagcccattt ctcagaacta agaattttat aaagaaactc atgttgttgt gcacggtttt
     4861 taacagtaaa gtcatgaccg gaaatttctt ttaatttttg aatcacagta gtttcatcag
     4921 caccgggttt cagcataatt tccattgccg atgcttttcc ttcggccgca ataaattcct
     4981 gaacaaagga catgggtgcc agaatatatt tagagtcaaa atcctgctga atggcaaata
     5041 ttccaccaat tgaaatgttc atttgtcgca atgcattttc aaaatcaccg ggagaagtaa
     5101 tgtttttatc aggaagataa agcattagag gctcgttccc tgtatctgca tttaccgaca
     5161 gtgcataagc aactccacta ccaacaatgg caaagtttcg ttcaccattt tccagaatcg
     5221 gtaaaccgat aaccacagct ttgttcaacc ctgttttttt catcagttcg ttgtcaaggc
     5281 ctttcaacgt aactatatcc tgtttttcac catagcgggc aaggcagttt tcttctaatg
     5341 taaatgctgc atattttatt ccgtcaatag cacggatttt ttccagatta agagcatcac
     5401 tgttaaaagt ttttcctttt atgactgtaa ttttaatgtc agggtcgaag gtgtcgtaaa
     5461 gtgaaattac cagatttcca aagccgttaa aaacactcag cactacaatt aatgccatag
     5521 tgcctgtaaa cacaccggca atagacacaa ggctgactaa gttgacaatg ttatgcgact
     5581 ttttgccgat gagataacga gatgctattt tgataatcaa attcatgcct gcgggctaaa
     5641 gtcagcaaga ataatcactt ttacaacaac gacttaaaaa atgcccctac ttttttacaa
     5701 ccttagcttt ctgttaatag gcaataatag agctaaatag tatcttcgct gcctcaaatt
     5761 tgtaatgtga tgaaaaaact ccacatggtt gacctgatca gtcaatatca aaaaataaaa
     5821 ccggaagtag ataaagctat ttcagacact gtaacatcat cagcctatat aaacggcccg
     5881 gaggttaagg catttcaggc agagttagaa aaatatttaa atgttggtca tgtaataaat
     5941 tgtgcaaacg gtacggatgc attgcagata gccatgatgg ctcttaatct tcaaccgggt
     6001 gatgaagtta ttactgcaag ttttacttat gtagctactg cagaggttat tgcactttta
     6061 aaattaaaac ctgttttagt agaggttatt cccggaacat tttccataga tcctgttgcc
     6121 attgaaaagg ccatcacacc aaagacaaaa gcaatagttc cggtacattt attcgggcaa
     6181 tgtgccgata tggaacccat tatgaaaatt gctgctaaac ataatcttta tgttattgaa
     6241 gatgtggcac aagccattgg tgcagactac actttttctg atggcagaaa acaaaaagca
     6301 ggaactatcg gaaccattgg ttgtacttcg ttttttccga gtaagaattt aggatgcatg
     6361 ggcgatggcg gtgctatatt taccaacgat gcagagcttg ccaaaaaaat aagaatgatt
     6421 gccaatcatg gtcagtctgt tcagtatgtg catgacgaaa ttggtgttaa ctcaagactt
     6481 gacagtattc aggccgctat tttgagagta aaattaaaac atcttgacag ttatgctgca
     6541 gcgcgacaaa aagcagcggc atattatgac aatgctttta gaggtagcac aaaaattaaa
     6601 acaccggaaa gaagcaaaaa ctctactcac gtttttcatc aatacacttt agttcttgat
     6661 ggtgttaatc gtgatgagtt gcgtcactat ttagccgaga aggaaattcc tgctatgatt
     6721 tattatccta ttccgcttca catgcaaaag gcttataaag atgatcgcta taaggaagga
     6781 aattttccgg taacagaaaa actttgtgcc aatgtgctgt cattacccat gcatacagag
     6841 ttggatgagg agcagttgga atatattacc cgtgcagtac ttgaattttg taaatagaaa
     6901 ataattttat ccgtatagta ttttaatatg aacattggag ttgtaggtac aggttatgta
     6961 ggtttggtta ccggaacctg ttttgccgaa atgggcaatc atgtagtttg tgtggacata
     7021 gatcaggcaa aagttgatag catgaaacaa ggaaaaattc ctatctatga gcctcatctt
     7081 gatgtgttgt tcgagaggaa tatcagacaa aatcgtttgt cgtttaccac gcagcttaca
     7141 gatgcagtta aacattcatc aattatattt cttgcattac ccacgcctcc cggtgaagat
     7201 ggctctgcag atttatctta tgtactgggt gttgccgacc aattaggaaa aatgctagat
     7261 ggttataaaa tcatcatcaa taagagtaca gtgcctgttg gtacagcaga agcagtgaga
     7321 gatgctattg ctaaaaacta caaaggcgaa tttgatgtca tctctaatcc tgaattttta
     7381 cgcgaaggtt ttgcagtaga tgattttatg aaacccgatc gtgttgttat tggaacacgt
     7441 tcagagaaag caaaaaaaat tatgggagaa ctgtatgcac cttttcttcg tcaaggcaat
     7501 ccaatatatt ttatggacga gcgcagtgca gagttaacta agtatgcagc aaactcattt
     7561 ctggcaacaa aaattacttt catgaatgag gttgccaatc tctgcgaaaa agtgggtgct
     7621 aatgttgatg cagtgcgcat tggcataggc tctgatgaca gaatcggtaa gcggttttta
     7681 tttgccggta ttggttatgg tggaagctgt ttcccaaaag atgtgcaggc attagaacac
     7741 acatccggca agtatggata tgattttagt attctgcaat cggtgatgag cgtaaacaac
     7801 aagcaaaaaa tgttgcttgc agaaaaagca ttggaatatt atggtggtaa tgtaaaagga
     7861 aaacgatttg cattgtgggg acttgcattt aaacccgata ccgatgatat tcgcgaagca
     7921 ccatcacttt atattattga catgcttaca aaagcaggtg caaccattag agcatttgat
     7981 cctgaggcaa tgaataatgt gaaaaaactg ataggtgata aaattgagtt tgctaacgat
     8041 gagtatcatg cattagaagg tgctgatgct ttgttgatag ccacagaatg gagtgccttc
     8101 agaacacctg attttgacaa aatgcattca gctttgaaaa gcaaagtaat ttttgacgga
     8161 cgtaatttat acgaaccaca aaaaatgcag gaagcgggat attattataa cagcattggc
     8221 cgccaaacgg taaaaccatg acaaaaagaa ttttaattac cggtgcagcc gggtttttag
     8281 gatcacacct gtgtgatcgt tttattcagg aaggttatca ggttgttggt atggacaacc
     8341 taattacagg cgatttaaat aatatagcac atctctttaa gctgcgtgag ttcgattttt
     8401 atcatcacga tgtttctaag tttgtgcacg tgcccggtaa gattgattac atattgcatt
     8461 ttgcatcacc tgcatcacct atagattatc ttaaaattcc aattcaaaca ctaaaggtag
     8521 gttcattagg tacacataac ttacttgggt ttgcattagc aaagaaagca cgcatattag
     8581 tggcaagcac ttctgaagtt tatggagacc cattggttca tccgcaaaac gaagactatt
     8641 ggggtaatgt aaatccaatt ggcccgcgtg gtgtgtatga tgaagcaaag cgttttcagg
     8701 aagccattac gatggcctat cacacctatc ataaattaga aacacgtatt gtacgaattt
     8761 ttaataccta tggcccacgc atgagactta atgatggtcg tgcactgcct gcatttgttg
     8821 gtcaggcttt gcgtggcgaa gaccttaccg tttttggtga tggcagtcag tcgcgttcat
     8881 tttgttatgt ggacgatttg gtagaaggta tttttcgttt gcttcactgc gactatgccc
     8941 aacccgttaa cataggaaat ccggtagaga taactatcaa acaatttgcc gaagaaatta
     9001 ttaagcttac aggcacaaca caaaaaatta tttacaagcc actgccgcaa gacgatccta
     9061 agcaacgtca gcccgacata acacgtgcac gggaaatatt gggatgggaa ccaaaagttt
     9121 cgcgcgaaga tggattaaag attacttacg aatattttaa gtcactacca ccggaagagt
     9181 tgaaacgctc tgcacatagg tttgcttgat ttgtaatttt tcttttattc caactagtat
     9241 gagacatttt cttcttcaaa gattataatg ataaacaaat agaagaaaaa atttatcatt
     9301 tataaccaat cgaattatgt attttatttc aataactcac tttctttttg ccttctaaca
     9361 caaaacctag tgtaaaatca ttttggaatt gaagccttgt actataaaga gggtcgtaca
     9421 ggacatgtgt tgcaatacga aattgaatgt attttaaaaa cttaaagata acagcattgg
     9481 caaaatcaag attaatttga ttcttgttaa taccattagc acctcctgtg gttactcact
     9541 gtacatagct tgttgaaaaa gtcaaacata aggtggttaa tagtataaaa aatatttttc
     9601 ttagatgatg tagttgtgtt gttattgatg tagtgtttaa tcctaagtag gcgcgagcgt
     9661 catttccgct cgtgcctttt atctttgcgg cctccggccg tccgatagct atcggacttc
     9721 atagttcagc cagttttaaa tctgtaaatt gattagcact gctatgcaca taaaaatgtg
     9781 gctcttgtaa aaacatcaat ccaaccaact gtgtttaata aaacaaagta taacccgtta
     9841 ggaaatattt tgtatttttt gctcatacac cggtaaagta aggcttatag tttgcaagat
     9901 gcaaacaaaa tgaatggcac gaacggaaat gacgctcgca cctactatgg attatttcct
     9961 cttttctttt tgcctgaaat attttcgaaa caaaaaattc ttttgtaagg cttttaaatc
    10021 ttcttcaaga agaaaggaac ttttctttaa ctgatttaat gtttcattaa aatcattttt
    10081 catgatagtg tcagtaagca aagcctgtac agtaccttgt ccattgacaa gtaggccgga
    10141 aaactgattg atgttatttg ttgcctcatt taaagaatcg gaaaattgtt gtaggttagt
    10201 aagcaagatt gctaattgat tttctgaaat actgtcagca ataagtttac ccaatacacc
    10261 tttgccattt tgaacatcct tggtaatatt attaatatct tttgtaaagt tatcaatgtt
    10321 atttgtagcc gaatttattg actgaataga ttgtcttaaa ttttcaacta cagcagaatc
    10381 ggagataagt gtaataagat tgttattctt attaaaacgc tgtactaagc ctttaagatc
    10441 agatgtaatc gcagccagat tatcgttagt tttatttaga gttcttatca tctcatccga
    10501 ttcgacagga cgcagtgaca ataatacatc acctttttca attggagaag atggagcagg
    10561 cagcgagttt atattaacaa gtttattccc catcaaccca tctgttccaa tactggcaat
    10621 ggcatttttc ttaatataag gagccatctt tttttcaatt atcattttta ctgtaatggc
    10681 tgaatcactt tcaaaaatga ttttctgaac agtaccaata tcaataccac cataacgtac
    10741 attatgtccg ggcattagtc catttacgtt atgaaatact gcacttatct caattgtttt
    10801 actgaaaaca ctgcgtttgc ttccaatcat atagagggta attaccaaca ctattagtcc
    10861 cgacattacg aagaacccta atttgatatt gtttgattta gtattactca tgatgcaaat
    10921 tcaaaaaatt gattaatatt tttatcagat gattttttta attcatcata cgttccttcc
    10981 gcataattcc tgccatcaag tagaacgata attttgttag ctgtaagttt tgctactgac
    11041 atatcatgcg aaatgattac tgatgaagta tgatactttt tttggaggtc gagcatgagt
    11101 tgtacaattt ctctacttgt tatcgggtcg agacctgttg taggttcatc ataaagaatg
    11161 agttttggtt tcataatcaa agtgcgagca ataccaattc gtttacgcat acctcctgat
    11221 aattcagaag gcatcatatt gatggcatga agaagcccca cattatccaa agattcttca
    11281 actagatttt ttacttcaga agaagttaaa gaaatccagt gtcttcgcat tgggaattca
    11341 agattttctc ttactgtcat tgagtcgtac aatgcactat tctgaaaaag aaatccaatt
    11401 ttaatacgaa ttttatctag cgactcctga tctaattcaa gcatgtttgt gtcaaaaaca
    11461 tgaatattac ctttgtcagg tttcagtaat ccaacaatac attttatcag tacagatttt
    11521 cctgagcctg attttcctaa tacaacaata ttttctccct taattaattc cagattaaaa
    11581 ttattcagaa ctacattact gccaaatgct ttacaaagtc cttctatttt cagaatgata
    11641 ttagtattgt ccatcatcag ttcaagccta ataaatcagt aatctgaaca gcaattaagt
    11701 caagaataaa aataattaat gaagctacta ctacagcaga atttgcagca tgtccaactc
    11761 cttctgttcc tttttgagaa ttataacctt taaaacaacc tacaagtccg atggttaaac
    11821 caaaaaagaa tgatttgact aatgcaggaa taaaatcact gtactcaagt gaattgaaaa
    11881 ccttgttata aaataatttc caacttacta catctttaaa attaacacca atatagcagg
    11941 cgtaaatagc aattaaatca gccagtataa caagtatggg taacataaat gcaatactca
    12001 aaacgcgtgt aacaactaaa aatttaaaag gatttgtgcc agaaacttcc atagcgtcaa
    12061 tctgttcagt aaccttcatg gaacccaatt cagctccaat gcttgagcca attttacctg
    12121 cgcagattaa tgcagttatt accggcccta tttcccgtac aattgaaata gcaatcattg
    12181 ccggcaacca tgatgttgca ccaaattctg ctaatgtggg tcgcgattgt acggtaagta
    12241 caagacccat aatgaatgct gtaataccta ctagtggcaa cgacttatag ccgtttataa
    12301 atgcttgttt aaaaagttcc tcccattcat agcgtggttt aaatccttct ttgaaaaaac
    12361 gaacaacaaa ggacgacaat tgccccgttt catgtaataa atcagatata cctttaggca
    12421 gcttcataat tcaaattcca agtaagaaaa acactttcat aaaggagcta agataaaaaa
    12481 ttaatcggag caatagttta ataaagtgaa taaacctgta ggttaaccac aatttactac
    12541 atttctaaaa acagagtttt cttagcatct ttgcacaatg gcagataaag aaattaccca
    12601 acccatacag accgattccg gcattgaagt aaaacaggtt tatacatcat gcaaaccaat
    12661 ggacgaacta cctgcaaagt ttccatttac acgtggagtg caagccgata tgtatagaag
    12721 caagttgtgg acaatgcggc agtatgcagg attttctact gcagaagaat ctaataaacg
    12781 ctatcactat ttgctgaaac aaggcacaac aggcttatca gttgcttttg atttgcctac
    12841 acaaattggc tacgactccg atcacgagct ttcagaaggt gaagtaggaa agtccggtgt
    12901 ggcaatagat tcattgcgcg atatggaagt attgtttgac ggtattcagc ttcagaacat
    12961 cacaacatct atgaccatca attcaacggc tgctgttttg ttgagcatgt acattgcttt
    13021 ggcgaagaag caaggagcag acctctctaa aatatcagga acaatacaga acgatattct
    13081 gaaagaatat gcagcacgcg gcacatacat ttatccgcct aaaccaagca tgcgcatcat
    13141 tacagatatt tttgatttct gcagtcgtga agttcctaag tggaatacca tatctgtttc
    13201 cggttatcat attcgtgaag caggaagcac tgctgtgcaa gagttggcat ttaccttagc
    13261 tgatggcaag gcttatctta aagcagcgat agatgcagga ttggatataa atgtttttgc
    13321 caaacgtatt tcttttttct tcaatgcaca caacaatttt tttgaagaga tagccaagtt
    13381 tcgtgcagcc cgtagaatgt gggcatctat cacaaaagag cttggtgcta cagaccctaa
    13441 agcaatgatg ctgcgttttc atacacaaac cggaggcagt acattaacag cacaacaacc
    13501 acaaaacaac attatgcgtg taaccatgca ggcattgagt gctgtacttg gtggaacaca
    13561 atcgttgcac accaatggtt ttgatgaggc attatcatta ccgactgaaa atgctgcacg
    13621 tattgcattg cgaacacagc aggttattgc ttatgaaagt ggtgtaactc aaacggtaga
    13681 tccgcttggt ggttcttttt atatagaatc attaactgat gaagtagaag ctaaggcttg
    13741 ggattacatc agaaaaattg atgatatggg aggcgctgtt gcagcaattg aagcaggcta
    13801 tattcagaat gaaattgcag agtcgtcata taattatcaa aatgcaattc aaacgggaga
    13861 aaaagtaatt gtgggagtaa ataaatttca ggttgaagaa ccggtttttg atgatatctt
    13921 ttctgttgat gacaccatca gaaaagtaca agtacaaaaa cttgcacagt tgcgcacaga
    13981 gagaaataat gaaaaagtta atcaggcatt aagcaactta gaatctgctg caaaggataa
    14041 tgccaatctt atgccttaca ttattcaatg tgttgaacac tatacaacac ttggagaaat
    14101 atccgataaa ctcagaaaag tttttggtga gtatcgttga taaaatttaa tgacattgta
    14161 aaattcaaat catatataat ttttaaaaat gaaaaaaaga attttaatta ctcttgctgt
    14221 ttcaacttta tttttcactg cttgtaagaa agataaagac agcaagcctg ctgaaagcga
    14281 gttatcgttt catctgcata ccttagtagg aaacactgct gccaactaca ccgatatctt
    14341 tactgataac accggaagaa aatttaatat tgctgattgc agatattaca tcagcaacat
    14401 tgttttgatt aaagatgatg gaacggaatt tccgttgacc gataaagcgc tattggcaag
    14461 tccatcacaa aaagaataca gtttgggaaa tgttccggtt ggaaaatata aaggttttcg
    14521 gttcttaatg gggttagact cagtgactaa tcatgacgac cctgcaggtt atgctgcatc
    14581 aaatcctttg agttatcaga atccaacaat acattggggc tggaacaatg gctacatctt
    14641 tatggagttt gtaggcaagt tcgattcaac agtcgctgca acaggaaatc cggtggcaga
    14701 ttatttttat cacattggtt ttgatgattt aaagcgtact attgatttta gcaattctcc
    14761 ttttgaaatt actactgatg ccggtttaga gattggtatt ttattcgacc ttaaaaaagt
    14821 actgaacaat gtagatttac gtacagagtt tggtacacat acaatggata atatgcccct
    14881 tgctgttaaa attgccaaca actggcaggg tgcttacagc attgaataat ttactatctt
    14941 cgatgtatta atattttcta tcatgaaaag tatcaagtct gttttgtgtt tagctgttgt
    15001 tggttttatt ttctctttta gctcttgcaa gaaagatgat tcagatgcta ataatatacc
    15061 caacaccgac accattggtg agttgtcttt taacttcaga cagtttgttg gcaacgacag
    15121 aatagtttac agtcaggttt ataatgacac atcagggcga ccctttaaaa ttaccaacct
    15181 gcgttattat atttcaaata ttgttttaat aaaggcaaat ggtctggaac ttccgcttag
    15241 tggaaaagtg ctgttggttg acaatgaaaa agagcaatat actcttggtg aaattcctgt
    15301 tggtgattat tccggtttgc ggtttatggt tggtattgat tctgcaacca atcacctcga
    15361 ccctgcattg tacgatgcat caaatccgtt aagttatcaa cccgtgagta tgcattgggg
    15421 ttggaacttt ggatatattt ttatgaaagt agaaggcaat gtagatacag atagtgttcc
    15481 tacaggaaat ctaaacggaa taatttcata cgatattggt agcgatccat tggtgcgtgt
    15541 gataaatttc aaccatattt tttctgtgca tggtaatatc aacacagtca actttaaatt
    15601 taatatcctg aaaatgttta gtggtattga tttagcaaca gaaagcatca cacatacaaa
    15661 tccgggtaga gatttagcag acagaattgc cagcaattgg cttcttgctt tcaccattga
    15721 accatgatta aaatacaagt atcaaaaatt tttattttat cagttattgt tttttggata
    15781 gtaagcattg tatcatgccg aaaagatgat gccattggca caggaaacga agtactggaa
    15841 ttggaagtac ctttaggatt tccctatcct tcaataccgg aagataatca acctacaaaa
    15901 aacagaattg ccttaggcaa aattctgttt tttgacccaa tactctctcg cgacagtacc
    15961 atttcttgtt ccagttgcca tcatgctgat aaaaaattta ctgacggact taaagtcgct
    16021 gaaggaattg aaggacgaaa gacattacgc aattcaatga caattctcaa cacggcttat
    16081 cagccaaata tgttttggga tggtggctca ccgacattgg aaacacaggt ttctgcacca
    16141 attgaagcgc acgaggaaat ggattttaat gtcatgaaag ttgttgaacg tttaaataac
    16201 catcctttat atccatcatt gttcaggaaa gcatataaca gcggaccatc agcatttaca
    16261 cttacaagag ccattgcaaa ttttgaacgt acactcttta caggcaaatc aaagtatgat
    16321 gcgtttcagt atgagaaaaa aagcgatgca ttgtctgctt cagaaaaaag aggtatggat
    16381 atattttttg gtgagagagc agaatgtttt cattgtcatg gtggatttaa ttttacggat
    16441 tttagcttta agaataacgg actttatgaa gtttatgcag attccggcag agcacgtata
    16501 acaactttgc atgatgatgt gggagagttt aaagtgccat cacttcgcaa tgttgcactc
    16561 acagcaccct atatgcacga tggcagtgtg gctactcttg aagatgttgt tgaacattat
    16621 aactcaggag gtaagccaaa ttttaataaa agttttctca tcaggccatt gaatcttaca
    16681 caacaggaaa aagaagactt ggtgaatttt atgaaagcat tatccgatca ataacaatct
    16741 catttaattt agcatcatga aaaatattgc attatacatt ttaacttttg gaatgacagt
    16801 attccttgca tcatgccata aagacgacag ttgcaatgcc ggtaccggtg gaaataattc
    16861 tataaaggca actttgttgc atcacactaa ggttataagc ccaaaagtgg gatatcctga
    16921 tacacttttt ataaaatacg acaccaaaga ttttccgggt gaaaatgctt ctgattttga
    16981 tgcttacttt gttaacagcg atactgctat aagcactatt gaaattacag gtttaaaatg
    17041 tggtgattat tatttgtttg gaaccggttg ggatgctgat atttctcaac gtgttaaagg
    17101 tggcattccg gtaactattg cagaagatgc aggcgtgctg acaaagaatg ttccggttac
    17161 ggaagattag tactaagaaa gatattttcc cacaataggc attcgtctgc ctaagccaaa
    17221 cgctttagat gaaatgcgca atacaggcga caactgtttg cgtttatact cattgcggtt
    17281 aaccatactc aaaatacggt ttactaaagt ttcatcatat ccgcctttta taatttcttt
    17341 cgggccttta cgcagttcta tatattcaaa caaaatttta tcgagaatat cataaggcgg
    17401 taaagaatca ctgtcctttt gccccggacg taattctgca cttggagctt tacttaaaat
    17461 attttcagga atgatttctt tttttctgtt gatgtatctg cacaattcat aaacttctgt
    17521 tttgtacaaa tcgccaatca cactcagccc gccacacatg tcgccatata aagttccata
    17581 gccaacggca agttcacttt tattgcttgt gtttaaaagt atagggccaa atttattact
    17641 cagtgccatc agcaatgttc cgcgtatgcg tgcctgaatg ttttcttcag taacatcaaa
    17701 aggcttttca ccaaacaatg gtttaagtgt tttcaaaaac gtttcataca atggatttat
    17761 ttccatttta tcaaccttga tgttcaggtt ttgacaaagt tgttcggagt ctgatattga
    17821 atgtccgctt gaaaaagctg aaggcaacat aactggcaaa atattttctg cacccaaggc
    17881 atcggctgcc aacactgtaa caatggcact gtccacacca cctgacaagc caagtatcgc
    17941 tttcttaaaa cccatcttag aaaaataatc tcttatcccc atcagtaatg cctgataaat
    18001 caattcatat ttttctgttt gcttttcaac aacaagagtg gtgttttttg tattcagata
    18061 aattatttct tctttaaaat aaggcccctc atgcaccaat ttacccgaag catcataagc
    18121 taatgagcca ccatcaaaga taatttcagt ttgtgcgcca acatggttca catacaataa
    18181 tggaatttta tactgattta catttcgctg caaaattgct tttcgctgat gtgcatgttt
    18241 gtaatcaaat ggcgaagctg caatattgat gattaaagaa ggtttttctt ttatcaactc
    18301 ttccatagga gaatgaatgt agagtggatc gtcttccaca ttccataaat cttcgcaaat
    18361 agttaaagca attttttctc cgttaaactc aatacattca aattttctgc ccggctcgaa
    18421 atagcgatac tcatcaaaaa tatcatatgt aggcaatagt gctttgttaa ctaaagcctg
    18481 aactttgcct ttatgcaaaa accatgcagt gttaaagaga tttttccctt cgggaacggc
    18541 attgcgcgat gggcaaccaa caatgactgc aatattctga ctgtgtgctg caattttttc
    18601 tatgctgtta taacaacggt tgatgaaatc atcaaactcc agaaagtcga gtggaggata
    18661 tgctgttata gccagttcgg gaaaaacaat cagttctgca ccggattttt cggcttgttt
    18721 gattgcctca ataatttttg aagtgttgtt ctcaaagttg cccacatgaa agttgagttg
    18781 ggcaagtgca atgtgcatat ttcgaaatta tcaggcaaag gtatatttat ataaataatg
    18841 ttacagcgtc tttctgatat atctttttaa tgtaatttaa tcttccgcac ggtcatttca
    18901 agaaatgaca gtgccacaaa taaaaaaagc tgcctacaaa gggcagcttt ttataaaatc
    18961 ttattgtaat atttaaaaca tcacacctaa atcaacagct aaaaagtttg acgttgcttt
    19021 tgaatgtttt tctttaacta catcaagaaa accattatta aaagttatac caaaaagtaa
    19081 ggctgttttt ccacctaatg aatattgtgt tccaataccg gcaactaatg ccatgttcaa
    19141 tgggttgata tccgattttt taaaatcacc atctgttctg atattgatac ccgttgtaaa
    19201 tcccacctta ccaaagtaag tcatataacc aatttcattg gttttcagct ttaaggttaa
    19261 aggaagttca atgtattgca atacggtttt cattttatag atgtccatat atttcaattt
    19321 gccaccacgg taggtaacat caatgccggt ttcaaatgca tagttgttgg taaaattaat
    19381 atcggcaatc agtccataag caaaccctat tttgctacca tcattatcta aattatcgct
    19441 ggctttaaac caggctaccg ttggtgaaat tttaagtccg aaattaaatt tgttgtcctg
    19501 cgcttttgca aattgaaaag cagctacaaa ggctattgca attagtgata cttttgctct
    19561 cattatttta tgttttttta attcaaggca aaattaatga aattttccac agtcagacaa
    19621 acagtttatc ttttactatc aacagtgctt atgctgggtt tttcttgtca gaacgacccg
    19681 catggagata aacatactaa agacacaatg accacacatt taaaatttga tgaagtgttg
    19741 ttttctgctg acagtattaa cccacagcat gtggtgcaat ggaaacaaca atatccttat
    19801 tttttcaatc tgtttgtaaa cagaataata catctgccgc caacagacag tgctgcactt
    19861 gcattaaatc ttcaaaaatt tgttggtgat aaagatgtaa aggacattca tgctcgggtt
    19921 gagaaagttt tttctgctgc cgataaagat aaattgttct ctgaaataga aggattttta
    19981 aacaattaca aaaaagagtt cggcatacaa ccggtaaaac atctggtaac ttttctttct
    20041 gcatttaact atgctgttat aactgcagac agtaccattg gtattgggct cgacatgtac
    20101 ttaggcagcg attgtgagtt ttatccatca ataggaatac ctcaatatgc ttatcaaagg
    20161 ttttcaaaag attatatttt gagtgatgtg ataaaaggct ggtatcagag tgaacatgat
    20221 gtggatgcag aaaaaaatga tttcctcagc cgcatgattt attatggcaa gcaattgtat
    20281 tttacttcag ttatggcacc acaagtgcaa gacactattc tgactggata cagcaaaaag
    20341 caattggagt ggtgcagcaa caacagcaaa cagatgtggg cattttttat agagaacaaa
    20401 cttttttatt ctacccgcga aatggaatac atgaaataca ttgcagacgg acatgccaca
    20461 caaggttttc ctgaaggggc acctgcacgc acagcacagt ggctgggatg gaagattgta
    20521 agcacctata tgaagaacaa caacgttaca ttaaaagagt tgcttgcaga aaaagatgca
    20581 cagcgaatac tcgaaaaatc aggatataaa ccttagttca aatttaaaat aacaacaatc
    20641 agaaagatga aaaaatcaga gataaaattt actgtaagct tagacgacaa caacaaacct
    20701 gtggcattgc attggaatgc cgaagacagc ggcatgcaag gcgacaaaga atgtaaagcc
    20761 atgatgcttg ctttgtttga taagaaagat caaactacca tgcgcattga cttatggact
    20821 caggaaatga tggtagaaga aatgcaacag ttcttttttg aaatgtatat gagcatggcc
    20881 gatactttcc agaatgcaac taacaataaa gatatggccg ataaaatgcg aaactttgca
    20941 agacagtttg gaaaagaagt gagtttgatt aagtaactat ttcttaattt cttattcttt
    21001 tttatttggc agaattctgt ttcactgcaa aggcaataat gtgtccatca ttatctgcaa
    21061 aatagcaaac tttatcgccc caatctctgt ctgcaatagg gctaataagt tttgctccac
    21121 attttagggc atttgcatat tcagcctcaa tatcgtcaac ataaaagtat aattcacatc
    21181 ttggaattcc ggtacctttg ttgggatgtg gcattttttc agaaatgatt tttgcaatac
    21241 cattatcagg catcaaccct aatttgcaat taggagaaag tataaactct gtcattcctg
    21301 atgcattcaa atccggaatt tttctaaaga gttttgaata gaatacagtg cttgcctgca
    21361 gattgtttac ataaagaata aattctgtgt atagaatatg tgttgtcatt ataggatatt
    21421 ctgaatccgt ttcatagtca ctgcgaaggg gcgggagatt atgaaactac aaaggtactt
    21481 agaaacttat gatattgaca acatcaaaga tgcgaaacaa tttttaaaga ctgtagtaga
    21541 tctatacaac agcgaaagac ctcacatgag tatcagtaac tttacaccaa acagtattca
    21601 tcattcaaaa acaacaatca aaacagaaag attatgaaaa aattattatc gaaaaattcc
    21661 tactattgta aatccaattc aggactaaaa atgaattgta aacttatatc aggattaatt
    21721 aattaaactg taaacttttt ttaggacgag tcatttgctt ttgctgcaac ccacgaaccc
    21781 aacgcaaagg caaaatcaaa agagccgctt tttgccaagg cttttaaaaa agcagcagtc
    21841 agaattatat ttttgctgat atttaagaac caaaaattgc aaagtaatta ttatggactt
    21901 tgatgcacta ttaaaacttc ttcaagattg gacaaaccga gttccgctaa tcattctagg
    21961 cagtggtgca tctgttccgt ttcatcttcc ttccatgtgg acacttggcg accacttgaa
    22021 gaagtcaatt tcatttgccg atgccgatga caagaaacag tttgaagaat ttaaaacaac
    22081 ctttgacatg gttggcgatt tagaaacgac tttgctgcaa ttacaactca gaccaaatgt
    22141 tctaaaagaa atcatttttg aaacttggcg attagtaaac aaacatgacc ttgaagcata
    22201 cgagaaaatt ctaaacaaga aagttgagtt tccgcttgct gacataacaa ggcatctcat
    22261 ttcagctgca cagagaaaac tttcaatcgt cacaacaaac tacgaccgac ttgctgaata
    22321 cgcagcaagt ttatcacatg cgttcatttg cacaggttat acgcaaaact atttcggaca
    22381 tttttccaag ttgatacatc aacaggattt gaccaagttg aacggcttta gcggacaagt
    22441 taacatctgg aaagttcacg gctcattgga ctggttcaaa tcaaaagagg aagttgattt
    22501 tcatttgcca ttgcgacaaa ctattccaac tgacttcaaa ccatctatcg taacacctgg
    22561 gctttccaaa tatttggaaa cgcaattaga accgttcaga acaatattta cag
//
LOCUS       DAOOOL010000028        22309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_178_length_22309_cov_71.620877, whole genome shotgun sequence.
ACCESSION   DAOOOL010000028 DAOOOL010000000
VERSION     DAOOOL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_22309_cov_71.620877"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..209
                     /locus_tag="PKU82_03935"
     CDS             6..209
                     /locus_tag="PKU82_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOZ82056.1"
                     /translation="MYLLKIKGTAKIPDYVQMRDDNFTLIAYFRADRPEKAIAKAGLS
                     DKEEYLLKIIAEIPFGKVQKIEI"
     gene            206..874
                     /locus_tag="PKU82_03940"
     CDS             206..874
                     /locus_tag="PKU82_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ82057.1"
                     /translation="MTDTIVSLQEANIYVKKNLILTGVNLHVQKNEFVYLIGKTGSGK
                     SSLLRVLFGDIKLNQGEGNVAGFSLNKLKNKEIPFLRRKLGIVFQDFQLLGDRTAKDN
                     LLFVLKATGWNNKQKTDDRINEVLQKVGLGTKGFKMPHELSGGEQQRLAIARALLNEP
                     ELILADEPSGNLDPETSNEIMNLFFELSKTRCSVIMATHNYSLIERFPARIIKLETGK
                     VLES"
     gene            939..1562
                     /locus_tag="PKU82_03945"
     CDS             939..1562
                     /locus_tag="PKU82_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOZ82058.1"
                     /translation="MTPKRILLLTATLLSSMASFAQFAFNPQVGLSVAKLRNLESDES
                     TKARVGWHAGADFRIGRRFYVQPGIYYWQTSTAFINDSLKIQTDLQRSDIKLNARIGL
                     NLLDDENAKIRFSIGPSYNVLISKSAKSDDLDKVTLGELEDNFNNGVFNFDAGLGVDF
                     WKISFDLGYSIGLSDALDNTAYYKSEARYGTFYFNVGLVIGNATSSK"
     gene            1640..3124
                     /locus_tag="PKU82_03950"
     CDS             1640..3124
                     /locus_tag="PKU82_03950"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOZ82059.1"
                     /translation="MHKNNFFKIFLLLLFVVLIACRKDQLLTDSSAKLNFSEDTVLFD
                     TVFASIGSTTQYLLVYNDHNRPIKISSIKLAGGSSSPFRMNVDGVAANSLNDVEIRAK
                     DSLWIFIEVTIDPTSDQTPFIVTDSIIFETNGNIQDVDLVAYGRNARFITPKYAITFP
                     DGSGLPYSIIGDGSPCGTVIEWDSLLPYVVYGYAVVDSCLTLKINPGTKIYFYNNSGL
                     WVYRYGNLQVEGNLEHPVLFTGSRLESSYSEVPNQWDRIWINEGGTNKINYAIIKNGL
                     FGVQAEDVQFDGIIGDAPTQLTLSNSTIRNMSIGLLARSFNITAYNNVISDCKSYCTV
                     FSQGGTYNLKQNTIANYWRYSQRSTPAFFMNDFVNDGSSNVHIPLNINVDNCIIYGNN
                     DNEIEKDLLDSLADTYSFKNNLIKVDEKTTPISVIPAFINTLKNQDPLFEDQSKYNFN
                     LKDNSPAIDAGDGSLLTFPELNTDLNGGTRPQGAAPDLGAIEKK"
     gene            3192..3614
                     /locus_tag="PKU82_03955"
     CDS             3192..3614
                     /locus_tag="PKU82_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOZ82060.1"
                     /translation="MDQNTLAQKVVNHMMEHDRFSQWLGIKCLVMEPGKSVLQMQIRE
                     EMLNGFGLIHGGIAFSLADSALAFASNAYGRLSVALECSISYPAPVHNGDIITATAEE
                     LSLTNRIGVYQITITNQNNIKVAFFKGTVYRTSKEYFK"
     gene            3619..4824
                     /gene="pcaF"
                     /locus_tag="PKU82_03960"
     CDS             3619..4824
                     /gene="pcaF"
                     /locus_tag="PKU82_03960"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOZ82061.1"
                     /translation="MKQAYIVDGIRTPVGNYGGILSSVRPDDMAAHVIKDLMARNTGL
                     APEKIEDVILGCANQAGEDNRDVARMALLLAGLPVTVPGETINRLCASGMSAVINAAR
                     AIRTGDTSCMIAGGVESMSRAPYVMSKSSSAFARDVQLFDTSIGWRFVNSRMKEMYGT
                     DSMGETAENVATQYKINREDQDLFALNAQMKAAKSQRDGRFECEISPVIIPRKKGEPI
                     VASNDEFIKPDTSIEILSKLKPAFKKDGSVTAGNASGVNDGACALLLTDEETLNQNKL
                     KPMARIVSSAVAGVEPRIMGIGPVPAANAALKKAGLSWNNIDIIELNEAFAAQALACI
                     RQWNLDDRDERINPNGGAIAIGHPLGMSGARIILTAAHQLALTNKQYALCTMCIGVGQ
                     GYAVIIERC"
     gene            complement(4831..5466)
                     /gene="plsY"
                     /locus_tag="PKU82_03965"
     CDS             complement(4831..5466)
                     /gene="plsY"
                     /locus_tag="PKU82_03965"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOZ82062.1"
                     /translation="MINAENITLLLAAYLFGSIPSAVWIGKVFFNTDVREYGSGNAGA
                     TNTFRVLGPKAGVPVLLMDIAKGWMAVQLATYFGEYMPATQQFINFKLTLGAAALLGH
                     IFPVFAGFRGGKGVATLLGILWGVNPPAAVICMGVFLLFFLTTGYVSLGSMAAAVSFP
                     FIVMWWLNETVTSMNVFAIAVPILVLVTHQKNIERLLKKTESRVYLFRRKQ"
     gene            complement(5543..6610)
                     /locus_tag="PKU82_03970"
     CDS             complement(5543..6610)
                     /locus_tag="PKU82_03970"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOZ82063.1"
                     /translation="MKPELEQEGSAQEHLEQDELFEHHRIVVDNGQEQVRIDKFLTTR
                     MANVTRNRLQVAIDSGSILVNDLPTKANYKVKPGDMIRVVLPYPVREIELLCEDIPLN
                     IVYEDKYLAVVNKPAGMVVHPAYGNYTGTLSNAMAGHFYPELKGQALPKDVFKPGMAH
                     RIDKNTSGLLVVAKDEDTLNYLALQFYERTINRNYIALAWGDLKQDKGEIEGHVGRNK
                     INRKVMDVFPDGSYGKYAKTHYEVLERFGYVTLIKLKLETGRTHQIRVHMQHIGHPLF
                     GDFEYGGDKILKGTTENKYKSFIHNCFELMPYQALHAQSLGFMHPINGERKFFEAELP
                     ENFKQLLEKWRNYTSSAHRDG"
     gene            6903..7766
                     /locus_tag="PKU82_03975"
     CDS             6903..7766
                     /locus_tag="PKU82_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ82064.1"
                     /translation="MRQLKITKQVTNRETYSLDKYLSEIGKLPLLTSEEEVRYARKIK
                     EGDQHALEMLTKSNLRFVVSVAKQYQNQGLTLGDLINEGNLGLIKAAYRFDETRGFKF
                     ISYAVWWIRQSILQAISEQARIVRLPVNKLGFINKINKTYAQLEQDFEREPSNEEIAT
                     VLHANLDEINDTLRSAGKHISMDAPLLQGEDGNMLDLIPTEDGQSPEVSLMSESLKRE
                     IERALTTLTPREADVIRLYFGLTPEPAMTLEEIGERFELTRERVRQIKEKAIRRLKHT
                     SRSKILKAYLG"
     gene            7891..8607
                     /locus_tag="PKU82_03980"
     CDS             7891..8607
                     /locus_tag="PKU82_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOZ82065.1"
                     /translation="MAYEFYDATIIDIIDENDVVKRYFIKVPDERPFSFKAGQFVMVD
                     LPIDSKYTNRSYSIASAPSADNIFELCIVLNPKGMGTPYMWENYKVGTTIKLSRVLGK
                     FQIPEPIDTDLCFICTGTGIAPLRSQVVDILNRKIPHKNLYMVFGNRWTKDILYRQEM
                     EQLEKDYPQFKFIPVLSRDNEGWTGRKGYVHQIYEEIFADKRPAKFFICGWADMLKEA
                     RQRLEAMGYDKKAIKFESYD"
     gene            8717..10732
                     /locus_tag="PKU82_03985"
     CDS             8717..10732
                     /locus_tag="PKU82_03985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ82066.1"
                     /translation="MKNFNALGIILISCFFTNAYAQKKTNSATVKPTKAVTATAPAAI
                     SDPVLVNVAGQNITRSEFERVYYKNNNKGTANDEKSIRDYMELFINYKLKVKEAESLK
                     MDTSAAFKDELTGYRKQLAQPYLTDKDVNDNLVKEAYNRLQNDVRASHILIKLSSDAL
                     PKDTLAAYNRIMKIREKIVKGADFEKMARDSSEDPSAKENGGDLGYFTGLQMVYPFET
                     AAYNTKPGQVSMPIRTRFGYHIIKVSDIRPAVGEIHTAHIMIKVNKSDNDSVQKEAKR
                     KIDEIYAKFKAGEKFEDLAKQYSDDKGSAVNGGVLPWFGTGKMVPEYEKASFALLKDN
                     DVSAPIKSAYGWHIIKRLEKRGIASFDEKKNELKNQIMRDSRNEMGRASLVAKIKKEN
                     NFKEFAANKDEYIKSLDTTLADGTYNDSTALKKTKPLFNLAGKDYTQFDFAAYLAKHQ
                     TKRKDITSQAIAHSQYENFVTDACIALEESQLDKKYPDFKALMDEYRDGILLFDLTDK
                     MVWSKAVKDTSGLEQFYEKNKNNYMWGERLDAVIYTCKNADVAKEARKMLKKKKSDSE
                     IAATLNKDSSLNINIKDGKYSKGDNEIVDKLEWKPGLSADLKDNNQVVFVDVKSKLAA
                     QPKKLDEARGLITADYQNFLEKSWITELRKKYSYSVNEDVLKSMFAK"
     gene            10744..11631
                     /locus_tag="PKU82_03990"
     CDS             10744..11631
                     /locus_tag="PKU82_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82067.1"
                     /translation="MKKQHITINGSGLLLAFAGTVFLMSCNNLPVDFKKDKNKNAIAR
                     VYDKYLYSYEIKDLVPKDATPQDSAMIVKSFVDDWIKQNIVLHQAENNLLDEQKNVEK
                     QLQEYRNSLVTFAYEQELVRQRLDTTVTDEEIEKYYNENNKNFLLKSNIIKATFIKAA
                     KKTPKIDKLKSWFNSSKEKDRQLLEKFCFQYAIDYSLNDNDWMQFDDLLKRVPIKTYD
                     KEEFLRNNRNIETADSANIYLVKITDFKTNESVSPLAFVKDNIKNLIINKRKLALIEQ
                     MQKSAFDQALKNNDFEIYK"
     gene            11648..12967
                     /locus_tag="PKU82_03995"
     CDS             11648..12967
                     /locus_tag="PKU82_03995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ82068.1"
                     /translation="MVFIMPSLLYAQKSDTVIDQIIGIVGNKIILQSDLEAQYQQYLA
                     QGHYKDESAKCDVLDQLLLTKLLIHYANIDSVTVSDAQVEGEIEKRIAYFTEQFNGST
                     EKLEEYYQKSVPEIKDEFRPLVKEQLLAQTMQQKIIGKASASPADVKAYFNSIPKDSL
                     PYINSELEYGEIAFKIAVSDEEKNKVKARLEEIRTRLLKGEDFATLAILYSQDAGSAK
                     NGGELGFVPRGQFVPEFEAIAYKLKKGEISPIIETKFGYHVVQLIDRRGETINVRHIL
                     LKPEVSNKDLLATKAKADSIAFDIRSGKMKFEDAAIQYSDAEDSKNNGGNVVNPATGN
                     TRFEANQVDAMVFFQLDKLKEGEVSNPALITSREGTQYYKIFLLKKRTQPHVANLTDD
                     YQRIQQAALSYKQAKLMDDWIVRKRKSTYVRIDPEYSHCEEIKKWSN"
     gene            12985..13953
                     /locus_tag="PKU82_04000"
     CDS             12985..13953
                     /locus_tag="PKU82_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOZ82069.1"
                     /translation="MNQQYTSDVEAIKGLVNSYQKLRTEIGKVIVGQDEVVRDVLISI
                     FSKGHCLLVGVPGLAKTLLVNTISQALGLSFSRIQFTPDLMPSDITGIEILDENRHFK
                     FIKGPVFANIILADEINRTPPKTQAALLEAMQERSVTVSGKRYELPNPFFVLATQNPI
                     EQEGTYPLPEAQLDRFMFNVFLDYPSFEEEVLVVKNTTGDKLGKAEKVLDENEITFFQ
                     DLIRKIPVPDNVLHYAVGLTAKTRPGAENKSAMINDYVAWGAGPRASQFLVIGAKCHA
                     AINGKFSPDIEDVKAVAVPILRHRLVRNYKAEADGVSVEKIIDSIL"
     gene            14105..15235
                     /locus_tag="PKU82_04005"
     CDS             14105..15235
                     /locus_tag="PKU82_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82070.1"
                     /translation="MRTLKLIGCGLLMTIIASCSSGDSSEQETDEGTETGTTTQATER
                     NFSGKNSNWAKLVAVENRDANGNLVSTIPLPSNWKIHNSRQQGDPTFTGPDGIVVKDY
                     PLQMFNYPFDQNTQYIYSQSGQQVRDFPGVEQVIQQDLIPVCRNQGLSFIKYYELPEV
                     SRIEKWYSDQLFQAVPMDKKTFAIGTDWQTADGNPYFLLVHFSISTTAQLRMWSYFVS
                     SLEADKDVFEKAKKQLLFSISNAHYELEPIMAYNRSEAEKAGRSWAQFNARMAQNQAN
                     FEASQRAHINKTNAINDAIMSGYRERNAASDRMQEQRIDGIYERTNVVDESGQKYKVE
                     SGYNQYWMNNDGEYISTDKVDYNPNLDDNMNDQKWQELKEIK"
     gene            complement(15244..16194)
                     /gene="ftsY"
                     /locus_tag="PKU82_04010"
     CDS             complement(15244..16194)
                     /gene="ftsY"
                     /locus_tag="PKU82_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOZ82071.1"
                     /translation="MAFFGLFSKKDKESLDSGLNKTRENFFTKITKAVIGKSTVDDEV
                     LDNVEEILVSSDVGVDTTLKIVKRIQDRVARDKYVGTDQLNTILKEEIAALLGENKSA
                     TVANLIDESKKPYIIMVVGVNGVGKTTTIGKLAYQFKQSGKNVLLGAADTFRAAAVDQ
                     LNIWAERVQVPIVSQGMNADPASVAFDTVKSGVAQNSDVVIIDTAGRLHNKVNLMNEL
                     TKIKRVMQKVVSDAPHEILLVLDASTGQNAIEQARQFTAATEVNALALTKLDGTAKGG
                     VVIGISDEFKIPVKYIGVGEKMTDLQAFDRVSFVDSLFAK"
     gene            complement(16277..16429)
                     /locus_tag="PKU82_04015"
     CDS             complement(16277..16429)
                     /locus_tag="PKU82_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOZ82072.1"
                     /translation="MAKKVVATLKTGKGKEFSKVIKMVKGANGAYSFKEEIVHNDHVK
                     DFLKEK"
     gene            complement(16447..16629)
                     /gene="rpmG"
                     /locus_tag="PKU82_04020"
     CDS             complement(16447..16629)
                     /gene="rpmG"
                     /locus_tag="PKU82_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOZ82073.1"
                     /translation="MAKKGNRVQVIMECTEHKNSGMPGTSRYITTKNKKNTTERLELK
                     KYNPILKKVTLHKEIK"
     gene            complement(16642..16875)
                     /gene="rpmB"
                     /locus_tag="PKU82_04025"
     CDS             complement(16642..16875)
                     /gene="rpmB"
                     /locus_tag="PKU82_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOZ82074.1"
                     /translation="MSRICDLTGKTSIVGNNISFSNRKTKRRFNPNLQTKKFFIPETG
                     EWVTLKVSASAIRTINKKGISACINSLIKKGHI"
     gene            complement(17024..18283)
                     /locus_tag="PKU82_04030"
     CDS             complement(17024..18283)
                     /locus_tag="PKU82_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ82075.1"
                     /translation="MTSVQSHLQQLNGNRIMGFSSSKKYFSWALALLLMAGCSSSRKT
                     AQMPPPAPAPKPVEQPVKKPEVVHEKTEKKEFNIGLMLPLNLEQQMQVFDDTSGSAMP
                     DYNNLSNLQFAEGALLAADSLKQLGINIKLNIAETPPDSFALWRLWRQNNFVKCEAVI
                     ASVPSSFNPAVARLSANNKQSLILTQPSNINFKANSEHVYMLSPSTYTQCMVAAKEIA
                     TRFPDSKYVLLARDVKPDKDIAQIFKDHLPAKRLKHFITSTVNPDSVCKILKTENAVL
                     IVTSSAEAYVNTILNQVNACIEKPVFAIGLPTWENFESIDFSILKNLRIAFFTSTYLD
                     YHAASTKSFRNNFINRYKTEPLLNAFQGYEAVMEYVKSNTSHGTLRAGYLPTLNIKYV
                     KDAANGIKENQGINLIEIKDYSLIPLE"
     gene            complement(18240..19766)
                     /gene="guaA"
                     /locus_tag="PKU82_04035"
     CDS             complement(18240..19766)
                     /gene="guaA"
                     /locus_tag="PKU82_04035"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOZ82076.1"
                     /translation="MHQTILIIDFGSQYTQLIARRLRELNVYCEIHPYNHLPEMSDSI
                     KGVIFSGSPFSVRDSQAPLIDTKSFFGKTPVLGLCYGAQLLAHQHQGEVVASKIREYG
                     RAILKINFDDEIFNDVSDNSQVWMSHADTIRSAGNDFEVIAQTDDVAVAAFRHKHLAV
                     YGFQYHPEVYHSTDGKKMLANFVYNICGCRGDWTPSSFVDETVAQLQDKLKNDKVILG
                     LSGGVDSSVAALLLHKAIGKNLYCIFIDNGLLRKNEAQHVMEVFKPLGLNIKCVDAAT
                     VFYDALKNISDPEQKRKTIGRVFIEVFDKEAHAISDVKWLAQGTIYPDVIESVSVNGP
                     SATIKSHHNVGGLPEKMNLKIVEPLRSLFKDEVRKVGKEMGLKEELLNRHPFPGPGLG
                     IRILGEISAEKVKMLQEADHVFISLLQKHNLYQKVWQAGAIFLPVNSVGVMGDERTYE
                     NAICIRAVTSTDGMTADWAHLPYEFLSIVSNEIINKVKGINRVVYDISSKPPATIEWE
                     "
     gene            19857..21794
                     /gene="thrS"
                     /locus_tag="PKU82_04040"
     CDS             19857..21794
                     /gene="thrS"
                     /locus_tag="PKU82_04040"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOZ82077.1"
                     /translation="MKITFPDKNVREYPDGTTAMDIAKSISEGLARNVLAAKINGQVS
                     DLSIPINSDATLELLTWDSQDGKNTMWHSSAHLMAEALEFYFPGIKFWVGPPVENGFY
                     YDVDLGGKQISSDDFKKIEDKMIELARKNNVYIRKEVPKQEAIAYFTEKNDEYKLDLL
                     QNLEDGNITFYTQGNFTDLCRGPHIPHTGYIKAVKLMNLAGAYWKGDEKNKQLTRVYG
                     ITFPKQKELDEYLAMLEEAKKRDHRKLGKELDIFMFDDEVGPGLPLWLPNGGVLIEQL
                     ENLAKRTENEAGYKRVRTPHLAKESMYLTTGHLPYYAESMFPPMEMDGEKYYLKPMNC
                     PHHHKIFGNLNASYRDLPIRLAEYGTCYRYEQSGELFGLMRVRCLQMNDAHIYCTEEQ
                     FASEFKAVNDMYLKYFKIFGIEKYIMRFSTHSPEKLGKKYVNEPELWLKTEDMVRRVL
                     IESNIPYIEVPDESAFYGPKIDVQVWSAIGREFTLATNQVDFAVPARFGLTYRDADNT
                     MKTPLCIHRAPLGTHERFIGFLIEHYAGNFPLWLAPMQVAILPISEKFNDFAKSVSQL
                     LNNYDIRALVDERGEKIGRKIRDAEVKKVPFMLIVGEKEMNEKLVSVRKHGEGDKGSM
                     TPEAFCQLVKKEIEDQLLINK"
     gene            21897..>22309
                     /gene="infC"
                     /locus_tag="PKU82_04045"
     CDS             21897..>22309
                     /gene="infC"
                     /locus_tag="PKU82_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOZ82078.1"
                     /translation="MNGRIHSPQVRLVGDNVEPRIYDTQTALDMAQDMGLDLVEISNT
                     SNPPVCKIVDYQKFLYERKKKDKEQKAKAAKSVVKEIRFGPNTDEHDFNFKLKHAIKF
                     LEEGAKVKAYVHFKGRSIAYKEKGEIILLKFAQEL"
BASE COUNT         7295 a         4149 c         4173 g         6692 t
ORIGIN      
        1 ccacaatgta tcttcttaaa attaaaggca ctgccaagat ccctgattat gttcaaatgc
       61 gcgatgataa ttttacactc attgcctact tcagagcaga caggccggaa aaagctatag
      121 ctaaagcagg actatcggat aaagaagaat atcttttaaa aataattgct gaaataccat
      181 tcggtaaagt acaaaaaata gaaatatgac ggacacaatt gtttcacttc aggaagccaa
      241 catttatgtt aaaaaaaatc tgattctcac aggagtcaac ctgcatgttc agaaaaatga
      301 gttcgtttat cttattggta aaacagggag tggaaaaagt tcattgttgc gtgttctgtt
      361 tggggatata aaattaaatc agggcgaagg caatgtagca ggattcagtc ttaacaaact
      421 caagaataaa gaaattccgt ttttaagaag aaaattaggc atcgtatttc aggattttca
      481 attgcttggt gaccgaactg caaaagacaa tctgctattt gtattaaaag ccaccggatg
      541 gaacaacaaa cagaaaactg acgatagaat aaacgaagtt ttgcaaaaag ttggtcttgg
      601 cacgaaagga tttaaaatgc cacatgaact ctcaggtgga gaacaacaac gacttgccat
      661 tgcacgtgca ttacttaacg aaccagaact tattcttgcc gatgaaccat caggaaatct
      721 cgaccctgaa acaagcaatg aaattatgaa cctgtttttt gaactcagta aaactagatg
      781 ttcagtgatt atggcgactc ataattactc attgatagag cgcttccctg caagaatcat
      841 taaattagaa accggaaaag ttttagagag ttaacaactt attttcctga atttccgtct
      901 gattgataag atttttttac ttttatcgtt tcaatcttat gactccaaaa agaattcttt
      961 tgttgacggc cacacttttg agtagtatgg catcatttgc acagtttgcc ttcaatcctc
     1021 aggttggttt gtctgttgca aaacttagaa atctggaaag tgatgaatct actaaagcac
     1081 gtgtgggatg gcatgccggt gccgatttca gaataggcag gcggttttat gttcaacccg
     1141 gaatctatta ttggcaaacc tcaacagcct ttattaatga ttctttgaaa attcaaacag
     1201 atcttcaacg aagtgatatt aaactcaatg ctcgtattgg actgaatctt cttgatgatg
     1261 agaatgcaaa aatcaggttc agtattggcc cgtcttataa tgttctaatt tctaaaagtg
     1321 ccaagagcga tgatttggac aaagtaactc ttggagaact tgaagataat ttcaataatg
     1381 gtgtttttaa ttttgatgca ggacttggag ttgatttctg gaaaatatct tttgatttag
     1441 gctacagcat cggcctttct gatgcactcg ataatactgc atattacaag tctgaagccc
     1501 gatatggtac attttatttt aatgttggac ttgttatcgg aaatgctact tcaagtaaat
     1561 aactcatctg ttttattaat gagaaaatat tttttagaga ttcatcaaat tccatccatt
     1621 ttatttagcc aaacaaaaca tgcataaaaa caactttttc aaaatattct tattactttt
     1681 atttgttgtt cttatagcct gtcggaaaga tcagttgctg actgattcat ctgcaaaact
     1741 caacttttct gaagacactg ttttatttga taccgttttt gcatctatag gaagtaccac
     1801 gcaatacctt cttgtttata acgatcataa tcgtccgata aaaatttctt caataaaatt
     1861 agcaggcgga agttcttcgc ctttcagaat gaatgttgat ggtgtagcag ccaacagtct
     1921 taacgatgta gaaattcgtg ccaaggacag tctttggatt tttattgaag taactattga
     1981 ccctacaagt gaccaaacac catttatagt tacagactct ataatctttg aaaccaatgg
     2041 aaatattcag gatgtagatt tggttgctta tggcagaaat gcacgcttca ttacaccaaa
     2101 atatgcaatt acatttcctg atggcagcgg actcccctat tctatcattg gtgatggttc
     2161 gccatgtgga actgttattg agtgggatag cttattgcct tatgttgttt atggttatgc
     2221 agttgttgac agttgcctga cactaaaaat taatcccggt acaaaaattt atttttacaa
     2281 caactccggc ttatgggttt atcgttatgg taatttgcaa gtagaaggca atttagaaca
     2341 tcctgtatta tttaccggat ccagattaga atcatcctac agcgaagttc ctaatcaatg
     2401 ggacagaatc tggattaatg aaggcggaac aaataaaatt aattatgcta taataaagaa
     2461 tggtttgttt ggtgttcagg cagaagatgt gcagtttgat ggtatcattg gcgatgcacc
     2521 tacacagcta acattaagta acagcacaat ccggaatatg agtattggct tactggcaag
     2581 aagttttaac attactgcct acaacaatgt tataagcgat tgcaaaagtt attgtactgt
     2641 attctcacag ggtggcactt acaaccttaa acaaaacacc attgcaaact attggcgtta
     2701 ttcgcaacgt agtacccctg cattttttat gaatgatttt gtcaatgacg gtagtagcaa
     2761 tgtgcacatt ccacttaaca tcaatgttga caattgcatt atctatggca acaacgacaa
     2821 tgaaattgaa aaagatttac ttgatagtct tgcagacacg tactctttta aaaataatct
     2881 cataaaagtt gatgaaaaaa ccacacccat aagtgtgatt cctgctttta tcaatacact
     2941 taaaaatcaa gaccctttat ttgaagatca gtcgaaatat aattttaatt taaaggataa
     3001 ctcccctgct attgatgcag gcgatggctc cttgctgaca tttccggaac tcaacaccga
     3061 cctgaatggt ggcacacgtc cacagggtgc agcaccggac ttaggtgcaa tagagaaaaa
     3121 ataattcttt ttttactaca cattttattt ctgtaatttc taccatctta ccaatcatta
     3181 catttgcagt catggatcaa aatacacttg cccaaaaagt agttaatcac atgatggaac
     3241 atgaccgttt cagtcagtgg ttgggaataa aatgcttagt tatggagccc ggaaaaagtg
     3301 ttcttcagat gcagattcgc gaagaaatgc tgaatggttt cggactcatt catggaggca
     3361 ttgcattttc gcttgccgac agtgcgcttg cttttgcatc caatgcctat ggaagattgt
     3421 ctgttgcttt ggaatgttca atatcctatc ctgcacctgt acataatggc gacattatta
     3481 ctgcaactgc agaagaactt tcactgacaa acagaatcgg tgtttatcag attaccatta
     3541 caaaccaaaa taatattaag gttgcttttt ttaaaggaac tgtttacaga actagtaaag
     3601 aatattttaa ataacagaat gaaacaagca tatatagttg atggcatacg tacgcctgta
     3661 ggaaattatg gaggcatact gtcttcagta cgtcctgatg atatggctgc acatgtcatt
     3721 aaagacctga tggcaagaaa taccgggctt gcacctgaaa aaattgaaga tgtaatttta
     3781 ggttgtgcca atcaggccgg tgaagacaat cgtgatgtag caagaatggc gctattgctt
     3841 gcagggctac ctgtcactgt tcccggtgaa accattaatc gtctttgtgc ttcaggaatg
     3901 tcggcagtaa taaatgctgc acgagcaatt agaaccggtg acacatcatg catgatagca
     3961 ggtggtgttg aaagcatgag ccgagcacct tatgttatgt caaaatcttc ttcggctttt
     4021 gcacgtgatg tccaactttt tgacacaagc attggctggc gttttgttaa ttcacgtatg
     4081 aaagaaatgt atggcaccga ctccatgggt gaaactgcag agaatgttgc aacacaatat
     4141 aaaatcaaca gagaagatca ggatcttttt gccttaaatg cacaaatgaa agcagccaaa
     4201 tcacaacgtg acggcaggtt tgaatgtgaa atttctcctg tcatcattcc tcgtaaaaaa
     4261 ggtgaaccta tagttgcttc taatgatgaa tttattaaac ccgatacaag catagagatt
     4321 ttatctaaac taaaacctgc attcaaaaaa gacggtagtg ttacggcagg aaatgcttca
     4381 ggtgtgaatg atggtgcatg tgcgcttctg ttaactgatg aggaaaccct taatcagaat
     4441 aaacttaagc caatggctcg catagtatca tcggcagtgg caggcgtaga gccacgcatt
     4501 atgggcattg ggcctgttcc tgcagctaat gcagcattaa aaaaagcagg acttagctgg
     4561 aataatattg acatcattga acttaacgag gcatttgcag cgcaagcatt agcatgtatc
     4621 agacaatgga atttagatga tcgtgatgaa cgcatcaatc caaatggcgg tgctattgca
     4681 attggtcacc cattgggaat gtcaggtgca agaatcattc ttacagcagc acatcagtta
     4741 gcactaacta ataaacaata tgcactctgc accatgtgca ttggtgttgg acaaggttat
     4801 gcagtaatta ttgaaagatg ctaaaggttg ctattgttta cgtctgaaaa gataaactct
     4861 gctctctgtt tttttgagta atcgttcaat gtttttctga tgcgtaacta acaccaatat
     4921 aggaactgct attgcaaaaa cattcatact cgttactgtt tcatttagcc accacattac
     4981 aataaatgga aaagaaacag cagcagccat tgagcctaat gaaacatagc ctgtagttaa
     5041 aaagaaaagt aaaaaaacgc ccatacagat tacagctgct ggcgggttca caccccatag
     5101 gatacctaat aaagtggcaa cacctttacc tcctctgaat cctgcaaata cagggaaaat
     5161 atgtcctaat aatgctgcag cacccaaagt aagtttaaag tttataaact gttgcgttgc
     5221 cggcatatat tctccaaaat aagtagctaa ttgtactgcc atccagcctt tggcaatatc
     5281 catcagtaat acaggcacac ctgcttttgg tcctaaaacg cgaaatgtat ttgttgcacc
     5341 ggcatttcca cttccatatt cacgaacatc agtattgaaa aacactttac caatccaaac
     5401 agcagaagga atagagccaa ataagtaggc tgctaataaa agagtaatgt tctcggcatt
     5461 aatcattcaa caaaaatcac acataaagat ttcaggcaaa tatattcttt ctgaaaatat
     5521 tgaaccgttg tagtcctttc tttcatccat cacgatgtgc tgatgatgta taattacgcc
     5581 atttttccaa caattgctta aaattctccg gcaactctgc ttcaaaaaat tttctctctc
     5641 cgttaatagg gtgcataaaa ccgagcgact gtgcatgcaa tgcctgatag ggcatcagtt
     5701 caaaacaatt atgaataaaa gatttatact tattttctgt tgtgcccttc agaatcttat
     5761 cgccaccata ttcaaaatcg ccaaacaaag gatgtccaat atgctgcatg tgaacgcgaa
     5821 tctgatgtgt tcttcctgtt tctaatttta gtttaataag tgttacataa ccaaaacgtt
     5881 caagaacctc gtaatgtgtt tttgcatact tgccatagct accatcggga aatacatcca
     5941 taacttttct gttaatttta tttctcccta catggccttc aatttcacct ttatcttgtt
     6001 ttaaatctcc ccatgccaat gctatataat ttctatttat ggtgcgttca taaaattgca
     6061 aagccagata gttaagtgta tcctcatctt tagcaacaac caataatcct gatgtgtttt
     6121 tatcaatacg atgtgccatc cctggcttaa aaacatcctt tggcaaagcc tgacctttta
     6181 attcaggata aaaatgccct gccattgcat tagacaaagt gccggtataa ttaccataag
     6241 caggatgaac taccataccg gccggtttat ttactactgc aagatattta tcttcataga
     6301 caatattcag tggtatatct tcacataaca attcaatttc acgaacagga taaggtaaaa
     6361 caacccttat catatcaccg ggcttaacct tataatttgc cttagtcggt aaatcattta
     6421 ccagaatact tccgctatct atggctacct gcaatctgtt gcgggtaaca ttggccattc
     6481 tcgttgtgag aaacttatct attctcactt gttcctgtcc gttatctact actatgcggt
     6541 gatgctcaaa gagttcgtcc tgttccagat gctcctgtgc acttccttct tgttctaatt
     6601 caggtttcat tcaccgcaaa tttaaaaaac atgtgcaaaa agattttttt tctgatttca
     6661 ttacataatt ttttcatcaa accttttctt tcgcttttct ctgcaagatc ttgtttttca
     6721 gagatgttgt caaggttttt atcataaaac tttcgcactt attcgatttg attctcattg
     6781 tttaaggtag aattattgaa attattccgc ttttcataca aataccattt ctctgtcttg
     6841 taaccgaagc agtgttgttg cgtttcactc tacataatta ttttcaggag aactattaaa
     6901 ctatgcgtca gttaaaaatt acgaaacaag ttaccaaccg cgaaacctat tcattagaca
     6961 aatacctcag cgagattggt aagttgccat tactgacatc ggaagaagaa gtaaggtatg
     7021 cacggaaaat taaagaaggc gatcagcatg ctttggaaat gcttacaaaa tctaaccttc
     7081 gatttgtggt ttctgttgct aagcaatatc agaatcaagg actgactctt ggtgacttaa
     7141 taaacgaagg taaccttggc ctgataaaag ccgcttatcg ttttgatgaa acccgtggtt
     7201 ttaaatttat ttcttatgca gtttggtgga ttcgccaaag cattctgcaa gctatttccg
     7261 aacaggcacg tattgtcaga cttcctgtaa ataaacttgg ttttatcaat aagataaata
     7321 agacttatgc acaacttgaa caggattttg aaagagagcc ttcaaacgaa gagattgcaa
     7381 ctgttttgca tgcaaacctt gatgaaataa acgacacact tcgttcggcc ggaaaacata
     7441 tttctatgga tgcaccactc ttgcagggag aagatggcaa tatgcttgat ttaattccta
     7501 cagaagacgg acaaagccct gaagtttcac tgatgagcga atcgcttaaa agagaaattg
     7561 aacgcgcact aactacatta actccccgcg aagcagatgt tatcagatta tatttcggac
     7621 taactcccga accggcaatg acattagagg aaattggaga aagatttgaa cttacacgcg
     7681 aaagagtccg acagataaaa gaaaaggcta ttcgcagatt gaaacatacc tccagaagta
     7741 aaattctgaa ggcttattta ggatgatttt tcattgtata tggttaatga cggtattctt
     7801 taattagggt accgtttttt attgtttttt gtttagcgat tttatttatt ttggtcgcga
     7861 aaattttaat cattctaaca caaagaattt atggcttacg aattttacga tgcaacaatt
     7921 attgatataa ttgatgagaa cgatgtagta aaacgatatt ttattaaagt acccgatgag
     7981 cgtccattca gttttaaagc cggacagttt gtgatggttg atttgcctat tgattctaaa
     8041 tatacaaaca gaagctattc tattgcatca gcaccttcag cagacaatat ttttgaactt
     8101 tgtatagtgc taaaccctaa aggtatggga acaccatata tgtgggagaa ttacaaagta
     8161 ggaacaacaa taaagctttc cagagtatta ggcaaatttc agattcctga gcctattgac
     8221 accgatcttt gttttatctg tacaggtaca ggtattgcac cattacgctc gcaggtagtt
     8281 gatattctca atcgtaaaat tccacataaa aatctataca tggtttttgg taaccgctgg
     8341 actaaagaca ttctctacag acaggaaatg gagcagttag aaaaagatta tcctcagttt
     8401 aagtttattc ctgttttatc gcgtgataac gaaggatgga caggtcgtaa aggatatgtg
     8461 catcagatat atgaggagat ttttgccgat aaacgccctg caaaattttt tatctgtgga
     8521 tgggccgata tgctgaaaga agcgcgtcaa cgcctggaag ccatgggata tgacaaaaaa
     8581 gccattaagt ttgagtcgta cgactaagca cattttcttc actttcattt ttgattcata
     8641 agcgaccgtc tattttagcc cgacttagct gtttaatcaa tttttttaaa tttgcacctc
     8701 cttaaatttc aagacaatga aaaactttaa tgcgttggga attattttaa tttcctgctt
     8761 ttttacaaat gcctatgcac aaaagaaaac aaacagtgca acggtaaaac ctacaaaagc
     8821 tgtaacggca accgcaccgg cagcaatcag cgaccctgtt ttagttaatg tggccgggca
     8881 aaacatcacc cgctcagaat ttgaaagggt ttattacaaa aacaacaata aaggcactgc
     8941 caacgatgaa aagtccataa gagattatat ggagcttttt atcaactaca aactaaaagt
     9001 taaagaagcc gaatctttga aaatggatac ttctgctgct tttaaagatg aactaacagg
     9061 ataccgtaaa caattggctc aaccctacct cactgacaaa gatgtgaacg acaacttagt
     9121 taaagaggct tataaccgat tgcaaaacga tgttagagca agtcatattc ttattaagtt
     9181 aagcagcgat gctttaccaa aagacacttt ggctgcatat aacagaataa tgaaaatcag
     9241 agaaaagatt gtaaaaggtg cagattttga aaagatggct cgcgatagct ctgaagatcc
     9301 ttctgcaaaa gaaaatggtg gcgaccttgg atattttaca ggcttgcaga tggtttaccc
     9361 ttttgaaact gctgcctaca acaccaagcc cggtcaggtg tcaatgccta tacggacacg
     9421 tttcggttat catattatta aggtaagtga cattcgtcct gctgttggcg aaatacacac
     9481 tgcacacatc atgattaagg ttaataaaag tgataatgac tctgtgcaaa aagaagccaa
     9541 gcgtaaaatt gatgaaatat atgccaagtt caaagccggt gagaaatttg aagatttggc
     9601 aaaacaatat tctgatgata aaggctctgc tgtaaatggt ggtgttcttc cttggtttgg
     9661 cacaggcaaa atggttcctg aatatgaaaa agcatctttt gcattgttaa aagacaacga
     9721 tgtttcagca cctatcaaat ctgcttatgg atggcatatc ataaagcgtt tagaaaaacg
     9781 aggcatagca tcatttgatg aaaagaaaaa tgaactgaaa aatcagatta tgcgcgacag
     9841 ccgcaatgaa atgggtagag cttctttagt tgctaaaatc aagaaggaaa ataactttaa
     9901 agaatttgct gctaacaaag atgaatatat taaatcgctt gataccacac ttgctgatgg
     9961 tacttataac gactctacag cattgaagaa aacaaaacct ttgtttaacc tggcaggtaa
    10021 agactataca caatttgatt ttgcagctta tctggcaaaa catcagacaa aaagaaaaga
    10081 cataacctct caggcaatag cccattctca gtacgaaaat tttgtaacag acgcttgtat
    10141 tgctcttgag gaatcacaat tagacaaaaa atatccggat tttaaagcat tgatggatga
    10201 atacagagat ggtattttac tattcgatct tacagacaaa atggtttgga gtaaggcagt
    10261 taaagacaca tcaggcttag agcagtttta tgagaaaaac aaaaacaact acatgtgggg
    10321 tgaacgctta gatgctgtaa tctacacttg taaaaatgct gatgtggcaa aagaggcaag
    10381 aaagatgctt aaaaagaaaa agtctgacag tgaaattgct gcaacattaa ataaagattc
    10441 atcactgaat ataaacatca aagacggaaa atattctaaa ggcgataatg aaatcgttga
    10501 taaacttgaa tggaagccgg gcttatctgc tgacttgaaa gataacaatc aagtggtttt
    10561 tgttgatgta aaatcaaaac ttgcagcaca gcctaaaaag ttagacgaag cacgtggatt
    10621 gataactgcc gactatcaga attttcttga aaaatcgtgg ataacagaat tgcgtaaaaa
    10681 atacagctat tcggtaaacg aagatgtttt aaaatctatg tttgccaagt aagaaacagc
    10741 atattgaaaa aacaacacat aacaattaat ggttccggct tgcttttagc atttgccgga
    10801 actgtttttt taatgagttg taacaacctg cccgttgact tcaaaaagga taagaataaa
    10861 aatgctattg cacgcgttta tgataagtat ttgtattctt acgaaattaa agaccttgtt
    10921 cctaaagacg ctacaccgca agacagtgct atgattgtaa aaagttttgt ggacgattgg
    10981 ataaaacaaa atattgtatt gcatcaggca gaaaataatc ttcttgacga acaaaaaaat
    11041 gtagaaaaac aattgcagga atatcgcaac tctttagtca cttttgccta cgaacaggag
    11101 ttggttcgtc agcgacttga cactacagtt acagatgagg aaattgaaaa gtactataat
    11161 gaaaacaaca agaatttttt gttgaagagt aatattataa aagctacttt cataaaagca
    11221 gctaagaaaa caccaaagat tgataaactt aaatcatggt ttaactcgtc taaagaaaaa
    11281 gatagacaac tccttgaaaa gttttgtttt caatatgcca ttgattattc tttaaatgac
    11341 aatgattgga tgcagtttga tgatttactt aaaagagtac ctattaaaac ctatgacaaa
    11401 gaagaatttt tgcgtaacaa cagaaatatc gaaactgctg attcagcaaa catatacctc
    11461 gtaaaaatta ctgactttaa aaccaatgaa agtgtttcgc cacttgcctt cgtaaaagac
    11521 aatattaaga acctgattat taacaaaaga aaacttgctc taatagaaca aatgcagaaa
    11581 tcggcatttg atcaagcact taaaaataat gattttgaaa tatataaata gaacatttct
    11641 gctcttgatg gtattcatta tgccatcgct gttgtatgca caaaaatccg atactgtaat
    11701 agatcagata attggtattg ttggaaataa aatcattttg caatctgacc ttgaggcaca
    11761 ataccaacag tatcttgcac agggtcatta caaagatgag tcagcaaaat gcgatgtctt
    11821 ggaccagttg ttgctgacca aattacttat tcattacgcc aatattgaca gtgttactgt
    11881 tagcgatgca caggttgaag gtgaaattga aaagcgcatc gcctatttta cagagcagtt
    11941 taatggctca acagagaagt tggaagaata ttatcagaaa tctgttcctg aaattaaaga
    12001 tgaattccgt cctttagtga aagagcagtt gcttgcgcaa accatgcaac aaaaaataat
    12061 tggtaaggca tcggcatcac ctgctgatgt caaagcatac tttaattcta ttccgaaaga
    12121 cagccttcca tatattaatt ccgaattgga atacggtgaa attgcattta aaattgctgt
    12181 ctccgatgaa gaaaaaaata aagtgaaagc aagacttgaa gaaataagaa cacgcttgtt
    12241 gaagggtgag gattttgcaa cacttgcaat cctttattct caggatgcag gctctgcaaa
    12301 gaatggcgga gaacttggtt ttgttccacg cggacagttt gtaccggagt ttgaagctat
    12361 tgcttataaa ttgaaaaaag gtgaaatttc accaatcata gaaacaaagt tcggttatca
    12421 tgttgtgcaa ttaattgatc gcagaggaga aactatcaat gtgcgccaca tattactgaa
    12481 gcctgaagtc agcaataaag atttgttagc cacaaaagca aaagctgaca gtattgcatt
    12541 tgatatcaga agtggcaaaa tgaaatttga agatgctgca atacaatatt cagatgcaga
    12601 agattcaaaa aacaatggtg gcaatgtggt gaaccctgcc acaggtaaca cacgttttga
    12661 agccaatcag gtagatgcca tggtattttt tcagcttgac aaacttaaag aaggcgaagt
    12721 aagcaaccct gcattaatca catcacgtga gggcacacag tattataaaa tatttctgct
    12781 taagaaacgt acccaaccac acgttgccaa tcttactgac gattatcaac gtattcagca
    12841 ggctgcatta agttataaac aagctaaatt gatggatgac tggattgtga gaaagaggaa
    12901 atcaacatac gtaagaattg accctgaata cagtcattgc gaagagataa aaaaatggtc
    12961 taactaacac aaaaaatatt cgaaatgaat caacaatata catcagatgt tgaagctata
    13021 aaaggattag tcaacagtta tcagaaactt cgaacagaaa tcggaaaagt aattgtcggg
    13081 caggatgaag ttgtacgtga tgtattaatt tctattttca gcaaaggaca ctgccttctt
    13141 gttggtgtac ccggtttagc aaaaacgctt ttagtaaaca caatttcaca agcattagga
    13201 ctttcattca gccgcattca atttacgcct gatttaatgc caagtgatat tacaggtatt
    13261 gaaatattag acgaaaacag acactttaag tttatcaaag gtccagtctt tgccaatata
    13321 attcttgcag atgagattaa ccgtacgcct cctaaaacac aggcagcact tcttgaagcc
    13381 atgcaggaaa ggtctgtaac cgtttccgga aaacgttatg agttgcctaa tccatttttt
    13441 gttctggcaa cacaaaatcc aatagaacag gagggtactt atccccttcc tgaagctcag
    13501 ctcgaccgat ttatgtttaa tgttttcctc gactatccct cgtttgagga agaagttctg
    13561 gttgtaaaaa acacaaccgg tgataaactt ggcaaagcag agaaagtgct tgacgaaaat
    13621 gaaattactt tttttcagga tctgattcgc aaaatccctg taccagacaa tgtattgcac
    13681 tatgcagtag gtcttactgc aaaaacacgt ccgggagctg aaaacaaatc tgcaatgatt
    13741 aatgattatg ttgcatgggg agcaggtccg cgtgcatcac agtttttagt tataggtgct
    13801 aaatgtcatg ctgcaatcaa cggtaagttt tctcctgata ttgaagatgt aaaagcagtt
    13861 gccgttccaa tacttcgtca tcgtttagtg cgtaattata aggcagaagc tgatggtgtt
    13921 tctgttgaaa aaatcattga cagcatcttg tagttttcta tttttcgtta atctcctcct
    13981 cttttcattc tcttcggaaa acatcaaatc cgcgatttct gactcatagc accaatcacc
    14041 ataattggtg attggtgatt atttctgata ttattagttt tacattttat taaactaata
    14101 aaagatgagg acattaaaat tgattggctg cggattactg atgacaatta ttgcatcttg
    14161 cagcagtgga gactcttccg aacaggaaac tgatgaagga acagaaactg gtacaacaac
    14221 acaggcaaca gaaagaaatt tctcaggtaa aaatagcaat tgggcaaaat tagttgcagt
    14281 tgaaaatcgc gatgctaatg gcaatttggt gtccacaata ccattacctt caaattggaa
    14341 aatacataac agcagacaac aaggcgatcc tacttttaca ggtcctgatg gaattgtagt
    14401 taaagattat ccgcttcaga tgtttaatta cccatttgat caaaacacac agtatatcta
    14461 tagtcaaagt ggacaacagg ttagagattt cccaggtgta gaacaggtca ttcaacaaga
    14521 tttaattcct gtttgccgca atcagggact ttcattcata aaatattatg agttgccgga
    14581 agtatcgcgg attgagaaat ggtatagcga tcagttattt caggcagtac caatggataa
    14641 aaaaacattt gctattggaa cagattggca aactgctgat ggcaatccct attttttact
    14701 tgtacatttt tctatcagca ccactgcgca actgcgaatg tggagttatt ttgtttccag
    14761 cctggaagca gacaaggatg tttttgagaa agcaaagaag caactcttgt tttcaatttc
    14821 aaatgcacat tacgaactgg aacccattat ggcttacaac agatcggaag cagaaaaagc
    14881 cggaagaagt tgggctcagt ttaatgctag gatggcacaa aatcaggcta attttgaagc
    14941 atcacaacgt gcacacatca ataaaacaaa tgctataaac gatgccatta tgagtggcta
    15001 ccgcgaaaga aatgctgcat cagacagaat gcaggaacaa cgtattgatg gaatttatga
    15061 aagaaccaat gttgtggatg aaagcggaca gaagtataaa gttgaaagtg gttacaatca
    15121 atactggatg aataacgatg gtgaatatat cagtacggat aaggttgatt ataaccccaa
    15181 ccttgatgat aatatgaatg accaaaaatg gcaggagtta aaagaaatta aatagcagta
    15241 atgctatttt gcaaaaagag aatcaacaaa actaaccctg tcaaaagcct gtaagtcggt
    15301 catcttctct cctactccaa tatattttac aggtatttta aactcatcag aaataccaat
    15361 caccacacca ccttttgcag tgccatctaa ttttgtcagc gcaagtgcat taacttctgt
    15421 tgcagcagta aactgtcttg cctgctcaat agcattctga cctgttgaag catcaagcac
    15481 caataaaatt tcatgaggtg catcggacac aactttctgc attacacgtt ttattttagt
    15541 caactcattc atgagattaa ctttattgtg caagcgacct gctgtatcta ttataaccac
    15601 atcgctgttc tgagccacac ctgacttaac agtatcaaat gcaaccgaag ccggatcggc
    15661 attcattcct tgcgaaacaa taggcacctg aacacgttct gcccaaatgt ttaactgatc
    15721 tactgcagca gcacgaaaag tgtcggccgc acccagtaac acattttttc ctgattgttt
    15781 aaactgatat gccaatttac caatagtcgt tgtcttgccc acgccattta cacctacaac
    15841 cattatgata tagggtttct tactttcatc aattaaattg gccacagtag ccgatttgtt
    15901 ctcgccaagt aatgcagcta tttcttcttt taaaattgta ttcaactgat cggttcccac
    15961 atatttatca cgtgctaccc tgtcctgaat tcgttttaca atttttaaag ttgtatccac
    16021 tccaacgtca gacgagacta aaatctcctc tacattatcg agcacttcat catcaaccgt
    16081 tgatttacca attactgctt tggtaatttt tgtaaaaaaa ttctcacgtg ttttatttaa
    16141 tccgctgtcg agtgattcct tatctttttt cgaaaacagt ccgaaaaatg ccatattatg
    16201 attcttttat tatagtatta aaacaaaaac agccttcttc cttagggaag aaagctgtca
    16261 ttcgatttca taaatattat ttctctttca aaaaatcttt tacgtgatcg ttatgcacaa
    16321 tctcttcttt gaatgaatag gctccattgg cacctttcac catttttatc accttggaaa
    16381 actcttttcc tttaccggtt ttaagtgttg caactacttt tttagccatt ttatttaagc
    16441 tttaaattat ttaatttctt tatgaagagt aacttttttc agaatggggt tgtatttttt
    16501 caactccaaa cgctctgtag tgttcttctt gttctttgtt gtgatataac gtgatgttcc
    16561 gggcataccg ctgttcttat gctctgtgca ctccatgata acctgaactc tgtttccttt
    16621 ttttgccatg atgctaaaag attaaatgtg tccttttttt atcagactgt ttatacaggc
    16681 tgaaattcct tttttattga tggtacggat tgcagaagca gatactttta aagtaaccca
    16741 ctcaccggtt tccggaataa aaaacttttt tgtttgcaga tttggattga atctgcgctt
    16801 tgttttacga ttggaaaaag aaatattgtt tccaacaata cttgtctttc cggtcaaatc
    16861 acatatacgg gacataacta ctcaatttta ttaagggagt gcaaaagtaa ttattttttg
    16921 tttaatgcca aatgcttttc ataatttttt actacagcaa ccatttcttt gctttacaag
    16981 acaataaact ataaatcaat tatttaactt attaaattaa gcactattct aatggaatta
    17041 atgaataatc tttaatttca ataagattta ttccttgatt ttctttgatg ccattggctg
    17101 catctttaac atatttaatg tttaacgtag gcaaatagcc cgccctaagt gtgccatgtg
    17161 atgtatttga tttcacatat tccattacag cttcatagcc ctgaaaagca ttcaataaag
    17221 gttcggtttt atagcgatta atgaaattgt ttctgaaact ttttgttgaa gctgcatgat
    17281 aatctaaata cgttgatgtg aaaaaagcaa tgcgcaaatt cttaagtatg ctaaagtcta
    17341 tgctttcaaa attttcccat gttggcagtc caatcgcaaa tacaggtttt tctatgcagg
    17401 cattaacctg attgagtatg gtattgacat aagcttcggc agatgatgtt actatcagaa
    17461 cagcattttc ggttttcaat atcttacaaa cggaatcggg gttcacggta cttgtgataa
    17521 aatgcttcag gcgttttgcc ggaagatgat ctttaaaaat ctgtgcaatg tctttatccg
    17581 gtttaacatc gcgcgcaagc agcacatatt tggaatcggg aaaccgtgtt gcaatttctt
    17641 tagctgccac catgcattgt gtgtaggttg acggtgagag catataaaca tgttcgctat
    17701 ttgctttgaa atttatgttt gatggttgtg taagaatcag gctttgttta ttgttggcac
    17761 tcagacgagc aacggcagga ttaaacgatg aaggcacaga ggcaatcact gcttcacact
    17821 ttacaaaatt attctgccgc cataaacgcc acagggcaaa actatcggga ggagtttcag
    17881 caatgttcaa tttaatattg attcctaatt gttttaaact gtctgctgcc agtagtgcac
    17941 cctctgcaaa ctgcaggttg ctcaaattat tataatctgg catagcactt cctgaagtat
    18001 catcaaacac ctgcatctgt tgttccagat tcaatggcaa catcaaccca atattaaact
    18061 ctttcttctc ggtcttttca tgaaccactt caggtttttt cacaggttgt tcaacaggct
    18121 taggtgcagg tgccggtggt ggcatttgcg cagttttcct actgctgctg caacctgcca
    18181 tcaacagtaa tgccaatgcc caactaaaat actttttgct gctactaaaa cccataatcc
    18241 tattcccatt caattgttgc aggtggcttt gaactgatgt cataaacaac cctatttatt
    18301 ccttttacct tattgataat ttcgttagaa actatactta aaaactcata aggcagatgt
    18361 gcccagtcgg cagtcatacc atcggtgctg gtaactgctc ttatacagat tgcattttca
    18421 taagtacgct catcgcccat tacaccaact gagttaacag gtaagaaaat ggctcctgcc
    18481 tgccacactt tctgatagag gttatgtttt tgtaataaag aaataaaaac atgatctgct
    18541 tcttgcagca tctttacttt ttctgcactt atttcaccta atattctgat tcctaacccc
    18601 ggacctggaa aaggatggcg attcaataac tcttctttta atcccatttc tttgcccacc
    18661 ttccttactt catctttaaa aagcgatcgc aaaggctcaa caattttaag attcattttc
    18721 tcgggaagtc ctccaacatt gtgatgcgat ttaatggttg ccgaagggcc atttacagat
    18781 actgactcaa taacatcagg ataaatggtt ccttgtgcca accactttac atcagaaatt
    18841 gcatgagctt ctttatcaaa aacttcaata aacaccctgc caatggtttt tcttttttgc
    18901 tctgggtctg atatgttttt taatgcatca taaaatacag tggcagcatc tacacatttt
    18961 atattcaatc ctaatggctt aaaaacttcc ataacatgct gtgcttcatt ttttcgcagc
    19021 aagccattat caataaaaat acaatacaga ttttttccaa tagctttatg cagaagcagt
    19081 gcagccacac ttgaatcaac acctcctgaa agccctagaa taactttatc attctttaac
    19141 ttatcttgca actgagcaac tgtttcatca acgaatgatg atggtgtcca gtcgccacgg
    19201 cagccacaaa tattataaac aaaattggct agcattttct ttccatcagt gctgtgataa
    19261 acttccggat gatactgaaa accataaacg gctaaatgct tgtgtcggaa agcagcaaca
    19321 gcaacatcat ctgtctgtgc tatgacttca aagtcattac ctgcactacg aatggtgtcg
    19381 gcatgcgaca tccacacctg actgttgtcg cttacatcat taaaaatttc atcatcaaaa
    19441 ttaattttaa gaatagctcg cccatactct ctgatttttg aagccaccac ttcaccctga
    19501 tgctgatgtg caagtagctg tgctccataa cacaatccca acacaggtgt tttaccaaaa
    19561 aaacttttgg tgtctataag aggcgcctga ctgtcgcgaa cagaaaatgg gcttccggaa
    19621 aaaataactc ctttaatgga atcactcatt tcaggcaggt ggttataagg atgaatctcg
    19681 caataaacat taagctcacg cagccttctg gcaattagtt gtgtgtattg tgagccaaaa
    19741 tcaatgatca gtatggtttg gtgcatgggc aaatgtaagc agaaaagcag aaacagaaat
    19801 atctgttttt tttagcgaca tggatagtta attatctttg cgcaaatttt tttagaatga
    19861 aaataacttt tccggacaaa aacgtcagag aatatcctga tggaaccact gcaatggaca
    19921 tagccaagag catcagcgaa gggcttgcac gcaatgtact tgcagcaaaa attaatggtc
    19981 aggtatcaga cctttcaata cctatcaaca gtgatgcaac tttggaatta ttgacatggg
    20041 atagtcagga tggtaaaaat acgatgtggc attcatcggc acatttaatg gcagaagcat
    20101 tggagtttta ttttccggga atcaaattct gggttgggcc tcctgtagaa aacggctttt
    20161 actatgatgt ggacttaggc ggcaaacaaa tttcaagcga tgactttaag aagattgaag
    20221 acaaaatgat tgagcttgcc agaaaaaata atgtctatat cagaaaagaa gttccaaagc
    20281 aggaagctat agcctatttc acagaaaaaa atgatgaata taaattagac ctgcttcaga
    20341 acctcgaaga cggtaacatt acattctaca ctcagggcaa ttttactgac ctatgtcgtg
    20401 gcccacatat tccacatacc ggttatatta aagcagttaa gttgatgaac cttgccggtg
    20461 cctactggaa gggtgatgaa aaaaacaaac agcttacccg tgtttacgga attacttttc
    20521 cgaagcagaa agagcttgac gaatatctgg caatgctgga agaagccaaa aaacgcgatc
    20581 acagaaaatt aggcaaagag cttgatatct ttatgtttga tgatgaagtt ggcccgggtt
    20641 tacctttatg gcttcctaat ggtggcgttt taattgagca attagaaaat ctggctaagc
    20701 gtactgaaaa tgaagccggc tacaagcgtg ttcgtacacc tcatttagca aaggaaagta
    20761 tgtatttaac tacagggcac cttccttact atgcagaaag catgtttcct ccaatggaaa
    20821 tggatggaga aaagtactac ctcaagccaa tgaactgccc acaccatcat aaaatatttg
    20881 ggaatctgaa tgcaagttat cgcgatttac ctattagact tgccgaatat ggcacctgct
    20941 atcgttatga acaatcagga gaattgtttg ggttgatgcg tgtgcgttgc cttcagatga
    21001 acgatgccca tatttactgt acagaagaac agtttgcatc tgagtttaag gcagttaacg
    21061 acatgtactt aaagtatttt aaaatcttcg gaatagagaa atacatcatg cgtttttcta
    21121 cgcactcgcc agaaaaatta ggaaagaaat atgttaatga gcctgaactt tggttaaaaa
    21181 ccgaagatat ggtcagacgt gtattgatag aatcgaacat accatatatt gaagtgcctg
    21241 atgaaagtgc attttatgga cctaagatag atgtacaggt ttggagtgcc attggtcgtg
    21301 aatttacatt ggctaccaat caggttgact ttgctgttcc tgcacgtttt gggcttacct
    21361 atcgtgatgc cgacaataca atgaaaacac cactttgcat acaccgtgca ccgctgggaa
    21421 cacatgagcg ctttatcgga tttttgattg agcattatgc aggtaatttc cctttgtggc
    21481 ttgcacctat gcaggtagca atactgccaa taagcgaaaa gttcaatgat tttgcaaaat
    21541 ctgtttcaca gttactaaat aattacgata ttcgcgccct cgttgatgaa aggggtgaga
    21601 aaataggcag aaaaatcaga gatgcggaag ttaaaaaagt accgtttatg ctgattgtcg
    21661 gggagaaaga gatgaatgaa aaactggtat ctgtgcgtaa gcatggcgaa ggagataaag
    21721 gaagcatgac acctgaggca ttttgtcaat tagtaaagaa agaaattgaa gatcaattat
    21781 taataaacaa gtaaaaagga ggctatatag caacatttaa caaaccaaat acgcccaaac
    21841 caggtggtcg ttttcctgac agaagattac cacgtcctaa agttgaagag caccgcatta
    21901 atggtcgcat tcattcacca caggttagat tggttggcga taacgttgaa cccagaattt
    21961 atgatacaca aactgcactg gatatggcgc aggatatggg tctcgatttg gtggaaatat
    22021 caaatacaag taaccctccg gtttgtaaaa tagttgatta tcaaaaattc ctttacgaga
    22081 gaaaaaagaa agacaaagag caaaaagcaa aggcagctaa atctgttgta aaggaaatac
    22141 gcttcgggcc gaataccgat gaacatgatt ttaatttcaa attaaaacat gccatcaaat
    22201 tcctcgaaga aggagcaaaa gttaaagctt atgttcactt taaaggacga agcatagctt
    22261 ataaagaaaa gggagaaatt atcctcctga agtttgcaca ggaattgga
//
LOCUS       DAOOOL010000029        22306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_179_length_22306_cov_78.916633, whole genome shotgun sequence.
ACCESSION   DAOOOL010000029 DAOOOL010000000
VERSION     DAOOOL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22306
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_22306_cov_78.916633"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1393)
                     /locus_tag="PKU82_04050"
     CDS             complement(161..1393)
                     /locus_tag="PKU82_04050"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOZ82079.1"
                     /translation="MKVPFAPPRMDQRVIDEVADTLLSGWITTGPKTKKFEKLLTAYC
                     NVPNVLALNSATAGLEIMLRWFGVKEGDEVILPAYTYSATANVVIHCGAKPVFVDVGD
                     DFNILPSEIENAINKNTKVIMPVDFGGLPCDFQKVNNIFQSENIKNKFVPATDVQQQL
                     GRILVLSDAAHSFGATVNNIKTGALTDVSVFSFHAVKNLTTGEGGAIALNLPKPFDNQ
                     SIYDYLCIYTLHGQNKDALAKMQKGNWRYDIIEAGFKCNMTDIMASIGLVELERYAET
                     LERRKQIVANYNEAFAKYPWAQLPEFKSNWRESSYHLYPIRIKNCTEKQRDAIMQKMF
                     DADIAVNVHFIPVPMFSFYTKLGYSLQHYPKAKDNYEREISLPLFYNLTEEQQQYVID
                     NLIKAVNETLVHRPAINS"
     gene            complement(1416..2672)
                     /locus_tag="PKU82_04055"
     CDS             complement(1416..2672)
                     /locus_tag="PKU82_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ82080.1"
                     /translation="MVQEKRALIFNYLFWLTGFLLPFDQPWINYALGLLLLFFVVQWL
                     TGKYQIKIQNPLLWLPLCYFLLHCIGMLYTQSQYTGWIELSAKSSFLIFPLLFSSNAI
                     SRDIIKTFKLFFIAGSGIICLYNLLLSFNQYIQHGNMSAFYYVAFSKGMHVQYLTIYL
                     NLAILFILEILFDASSQTKPIKKAYWVLLVLFFLVNITLLASRLATVVSYATILFYTV
                     VRIISSPNYLRYSILAALFAASVLIIDYFAFKKINRFEQVEIFLKENTNYVDFSKTEY
                     NSTTLRIPLWINAYEVIQRNPLFGVGTGDVKSSLDSMYLKNKFTYAHQNHFNPHNQYL
                     QTGVAFGLTGISLLMLMMLIPLYYAIRNRHYIFIAFQCIFLLNMLTESILERQAGIIL
                     FCLFYAIFSSEYVAAALKTVPEQNNT"
     gene            2820..3224
                     /locus_tag="PKU82_04060"
     CDS             2820..3224
                     /locus_tag="PKU82_04060"
                     /inference="COORDINATES: protein motif:HMM:NF023145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agenet domain-containing protein"
                     /protein_id="HOZ82081.1"
                     /translation="MKKLIFTSLFFLGFLTFTYAQKVNEKVQINSKGAWYPGKILKIN
                     ADDHTYYVSYDGWDEGWNEWVTIDRLKDFAKEAPAQPLTKFKVGDRVEVEYGMVPEPA
                     TVIEVGENKYHIKYDKTVFKDRWVTEREIKKL"
     gene            3285..4481
                     /locus_tag="PKU82_04065"
     CDS             3285..4481
                     /locus_tag="PKU82_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82082.1"
                     /translation="MIKEIRDKYLREFKEETYSEFLAYYDNLYNRKVEFRIAESPVFI
                     PKKLKDLLIEAGNEILSQSLTPEYLLKSEQAIPHGLKVPNEHKHPHFIAIDFAICKDD
                     NGNYLPQLIELQGFASLFFWQDLLPRTYAKYFQIPEGFSHFFDGWNTEKHRALMRKVI
                     LGNHKPENVILLEIEPEKQKTWIDFWETKTHTGVEPVCISKVIKEGKNLFYELNGVKT
                     PIYRIYNRIIFDELLQRKDLIKDFNLTDDVDVEWAGHPNWFFRISKYTMPFIKSRFAS
                     ECYFLSDVDTSKLSLADFVLKPLFSFAGSGVLIDVTSSDIESIPLEKRNEFLIQRKVN
                     YVPCMETPDGAAKVEVRLLYIWDDAETKPQLVLNLTRLSKGAMIGVSHNKNKTWVGGS
                     SSLMEI"
     gene            complement(4478..>5401)
                     /locus_tag="PKU82_04070"
     CDS             complement(4478..>5401)
                     /locus_tag="PKU82_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOZ82083.1"
                     /translation="KVGDVMRGNGSGILRMQISQAGEFSMYGDYNILAGDYLFTLQNI
                     VNKRFKIQSGLIQWAGSPYDATININAKYNLRASTFDLIPDSSNRSRVPVEVLLQLKN
                     NLMSPDVIFDVNVPNATPAVRTSLSSILGNEREMNRQVFSLLVLNRFSPPEGKNTSEE
                     SGSGNAVGQNLSEFVSQQLSNWASQLSDKFNIGVNYRPGDALNRDEFDVSISTELFRN
                     RVAVESNVGVANNSNQTSSIIGDFQVEFKISKDGNLRAKAFNKTNTNNLINNLNSQYT
                     QGIGVFYRKEFNTVSDLVESFRKRKKAAAPN"
     assembly_gap    5402..5501
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5502..8695)
                     /locus_tag="PKU82_04075"
     CDS             complement(<5502..8695)
                     /locus_tag="PKU82_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82084.1"
                     /translation="MLLLLFLFSVSLRFQKVQSWVVDRVTGYLSSELNTTVHIGGVRF
                     VLIKSVYLDDVFIADKQQDTLLYAKHLTATVAFQSLFIRNKKLILDEVQLDKARIALS
                     KSKSTRDMNFDFIIDYFSSPSKDTTSSPFYFAVNRVILSNIHFTYRDFKWDDKTPCID
                     FEDIDVSDFNGTLYNIKPTDSSFSFYGSKISLKEKSGFKINSFSTYAAFYNDSMSFNK
                     ILLRTPYSSIDAGHYSMSYNSMTDFEDYINKVMMKGEFNESTISSNDIQYFAEELFGL
                     DKRVEFSGTARGTVSNLKTKKLKIAYGKSTRFEGDVSLNGLPDVNTTFINLNIKHLST
                     NKTDIETIKQYPFTEQQYIKLPDNISELGKIVYRGVFTGFYNDFVSYGNITTAIGNLS
                     TDVNLKFKNKANESTYSGKLTADNFDIGKFWRLGNAVGTISFNAQLNGTGFTSEKVNA
                     RLTGLVSHVNLNGYDYSNINLDADVARKLFNGTLNMNDRNAQVSFKGSIDFREKLPLF
                     DFNAIVKNAALTKLNLLKRDTSALLSTEANLNMRGFNFDEFEGKLQLKNISYSEEYKS
                     AFVEKILIQSGMENNNRNLNVNSDLLDLNINGQYILSKIVYSFGHLLNCHLPPFMLKL
                     SPDVEKTNTIFSFSGNIKDVNPVLNIFTPGLSVSNQTEFSGRLNRQHCEIEVDATSPW
                     INYKNFENKNIKLTSNNKNNLLNIDLSASQVKISDSLILKNILLDAETQKSKSSIVLT
                     VANDSLSTSKMRLATEQTYTNGATRFHLLESLLQFKGEQWQVDPANEILFDSASISFD
                     KVLFTNKSQSLMLQGKVGNNKEDVLSVNLFKFKVPLINDLLAMYDIKLGGAASGNLNF
                     SSLYQRAIITGEMRVDSFAFNDEPLGNAIVNFDWLTDQKKLHIEGAIEQNNRNSAVVK
                     GDYIFQPKNDELSFNIKVDKLSITPLERYLNGFASKIVGLATGELNLTGLAKAPNLTG
                     KARLQRASMVIDYLNTRYSVLPEQDIIFDEKYIAFNKVGISDDQNHKGIVDGKIFHNH
                     FNDFTFSLRVDANKLQCLNTNASQNELFYGKAFAT"
     gene            8941..9978
                     /gene="tsaD"
                     /locus_tag="PKU82_04080"
     CDS             8941..9978
                     /gene="tsaD"
                     /locus_tag="PKU82_04080"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOZ82085.1"
                     /translation="MTVTDKKDIIILGIESSCDETSAAVSRNTQILSNVIANQTVHQK
                     YGGVVPELASRAHQSNIVPVVDQALREAAINISDVDAIAFTRGPGLIGSLMVGVSFAK
                     SAAAALNKPLIEVNHMQAHVLANFLSLEGQEGKTPSFPFLCLTVSGGHTQIVRVTSPQ
                     QMEIIGQTLDDAAGEAFDKIAKVFELPYPGGPLIDRYAQSGNPKAFNFPVANLPGLNY
                     SFSGLKTSVLYFLKKQLAQNPAFIAENRDDLCASVQYTIVKTLILKLKKAALETGISN
                     IAIAGGVSANSGLRKAVTEEAVKNNWQVHIPPLSLCTDNGGMICLAAYFKYLAADFST
                     LDVAPSARFGF"
     gene            10087..10971
                     /locus_tag="PKU82_04085"
     CDS             10087..10971
                     /locus_tag="PKU82_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82086.1"
                     /translation="MENQSKGNNRIWIVLFAASALFNVYQWRNHTSTTEVFEVTKDSL
                     ITARVDVEKELGATIEELNKYKGINDNLDSLLTEANHKIDEQKERIQKLIRSEGNSAA
                     LNKKLMAELESLKKLRDEYLDKIDTLMVENQKLKDDNAQLSGTVETLSKNLETTVNTA
                     SVLKSEYFKVSTFKKKGNGKFVSTALSKRTNKMDVCFSVLENKIAKAGEKNVYLRIIE
                     PGGKVMGNRSAGSTSFKMDSGEEVMCTVVSSLRYENATVDNCLSYEENDRIFTPGTYL
                     IEVYIDGNLSGASSYVLR"
     gene            complement(11342..11845)
                     /locus_tag="PKU82_04090"
     CDS             complement(11342..11845)
                     /locus_tag="PKU82_04090"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ82087.1"
                     /translation="MSEIVLRSLQIDDAEIFALHANNSAIADNMTNQFPFPYTKAHAL
                     EFIRRVNGFKPAQVFAIDLDGEAIGAIGLHPQTDIFLKNAELGYWLAEKYWGKGFVSI
                     AIKKIVEYGFSSFEIDRIFARPFGSNKASQRVLEKTGFVLEARLEKIIYKNNRYEDEL
                     IYAIRRK"
     gene            complement(11852..15877)
                     /locus_tag="PKU82_04095"
     CDS             complement(11852..15877)
                     /locus_tag="PKU82_04095"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82088.1"
                     /translation="MKKFVYYILLFLLPVTAWSQCMMTPVSLQDRISNATVVVEATAL
                     QQNSYWNNSNDFIYTSTTLSISAVYKGTIQGSQIALITEGGIVGNIMIKSEPSLNIKP
                     GQNGIFFLIPHSQPSGSKLSGMFEGYALSQSLILFDETSNSATGPFENYSDINALRNQ
                     IINATGGVQRISSIAPQQAPPSVNKIMIPPTIASIVPATTTAGTFSTITINGNNFGAT
                     YVNGTSNVEFPDANTGGVSYISAPQNHIQSWSNTQIQVWVPTQGGSGLIRVTNNLGET
                     TTSAISITINYNETNVVSGSNYYQPDLINNNGTGGYTFRYNTTFNGNAAAVAAFERAL
                     QTWRCGTFVNFNRSGTTATTCQALDGTNLVTFDGSCALPAGVLGISYSYYSACATGVW
                     YLNENDLKFRTNGTGGINWNYGPAATGGGNFDFESVCLHELGHSHQLGHTIVPVTVMN
                     YAIGPNTDRRTLTPISETAGGNDIMSRSVVTNACGPTAMVALSAGSCAINAPIADFNG
                     TPLTGCNTLNVTFTDASINTPTSWTWSFPGGVPNTFVGQNPPVIFYAAPGNYAVTLTV
                     SNAFGSNTLTKTNYVQIATCPPPTPDFTANPTTVCQGNTVQFVDLSTNTPTSWSWTFP
                     GGTPGTSTAQNPVITYATAGTYNVTLTSTNAYGSNTLTKTNYITVNNCPAPPVADFSA
                     SPTTLCSGGTVNFTDLSTNTPTSWSWTFAGGTPLSSVAQNPTITYNTPGTYAVTLIAT
                     NGGGSNTKTVSGYITVNLCSAPVASFSGFPTTICEGQAVTYVDQSTNSPSSWAWTFTG
                     GAPAASALQNPVISYATAGTYTVSLTATNAFGNNTFTRTNYINVVTCPSPGTGLIVND
                     GSLIFVQPTAMMHVEGGVINQDNLSNIGTWDNSGQVRIQGDWTNNSSGNAFINSSPGE
                     VELFGANQTIRGSTPTFFFTLNLTGTGIKTQNIDATVQGLLKLNDRELATQGNVMHVT
                     NTAPAAITRTGGLNSTPVQGFVSSTGSGKLWRNTNTTSVYLFPVGSSIPPARFRPVEI
                     KPLSATPNTFAVRMVNNDPNTDGFNRAVREPTLGAVNAGWYQKINRVSGSSLAEFSLY
                     ADDAQDAIASWPLLKLCQWGQAAPPVWWKDIGGAVTPGASPVLGSVLRTNWNNFSNTE
                     NFNIAPESNPLPVELLSFTGNCKNNIVNLNWTTASEINNHYFELQQSADGNDFVTIGR
                     VEGNGTTIKQNFYSFTIDQKTEHTFFRLVQYDFDGRANVLPAILVSCNTESPATVNIN
                     LMPNPAKDVFVSDIYLPEKTQLSMKVFNTIGQLVMEEQWTLDAGYHKQATQVSTLAKD
                     IYNIRYTSNSFNIVKQIAIVK"
     gene            complement(15911..17572)
                     /locus_tag="PKU82_04100"
     CDS             complement(15911..17572)
                     /locus_tag="PKU82_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82089.1"
                     /translation="MKKLFTVLTILLIASTNQAQNVGIGTNVPMQKLDVIGKIRSSTL
                     TGVGNRFVMADPTGTLNVSSSGLLDSTAWLTLGNSSIDTTKNFLGTLTNTHILFKTNG
                     NAAANERMRIRSGGEIIVNNTSVGNNNGDVFSVYATGSSNGINNALSPIGAFALNGYS
                     ATTGIGVSGANTGTGVGVWGSSSSGYGTVGFSGGNTAFAGMFQNSNANGTGLLVTGNN
                     LPGTYLVNGSAGSFRGTNFGIINFSNISSAAVAGSAVWGSNRKTIITSYLGGAGVTGI
                     DSAYGSGVIGVSFFGGNNGVTGIGSSNSGTGVMGQAGMGPTPYAVWGDVTTVSGTNAT
                     SIAVIGSNNSVGANAIGILGQEVAAPNGPTRYAIFANGDLAASGTKTFVIDHPLDPSG
                     KFLKHFSIESNEVLNYYRGNVVLDANGEATIQLPSYFNSINNSNYNYNLTPIGAPAQL
                     YVKSEVTGNNFVIAGGVAGMKVSWTLTAERNDPYLQQNPDSRLVELPKTGDAAGKYLV
                     PSLYGQPESAGIYYKPIPQKTAVNEKSKDAPLIKSRDFTQQNDSK"
     gene            17754..18812
                     /locus_tag="PKU82_04105"
     CDS             17754..18812
                     /locus_tag="PKU82_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82090.1"
                     /translation="MKRFKYLLILLPFSLNFALAQDVDTIENKHLINIKQLTFGGDNA
                     EAYFSFDNNKLAFQSNNKAWGVECDQIFLLDLNNWKSDSKPPLISTGKGRTTCSYFMP
                     GNKTILYASTHLRGDKCPETGDLRRGGKYLWPIFNTYDIFVADLNGKIVKQLTNAPGY
                     DAEATVSPDGKKIVFTSDRTGDLELWTMDIDGSNQKQITFGLGYDGGAFFSPDSKQLV
                     FRSSRPTTAEEIQEYKQLLSEGLVAPTNMEIYTCNVDGSNLKQITHLGRANWAPFFHP
                     SGKKILFSSNHESKSGFNFQLYMINTDGTGLVQITDQSVFNAFPMFSPDGKKLIFSSN
                     RNNGGTHDTNLFIADWKD"
     gene            18854..19468
                     /locus_tag="PKU82_04110"
     CDS             18854..19468
                     /locus_tag="PKU82_04110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOZ82091.1"
                     /translation="MLIEINENNIDDRKIKKVVECINNDGIIIIPTDTVYALACSIYS
                     LPAIEKVSRFKGVKPEKANLSFLCNDLKNIAEYTKPFDRSVYKLLNRSLPGPFTFILE
                     ASTKVPGIFRARKKNIGIRIPDQKVTLSIIQQLGHPLMSASLHDVNDPITDYLTDPDE
                     IYERFSDMVDMIVDCGAGGNVPSTIVDCTTDEITVVREGKGVLS"
     gene            complement(19465..20106)
                     /gene="nth"
                     /locus_tag="PKU82_04115"
     CDS             complement(19465..20106)
                     /gene="nth"
                     /locus_tag="PKU82_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOZ82092.1"
                     /translation="MLLKERYKYVIGYFRKNMPVAETELVYKNPYQLLVAVILSAQCT
                     DKRVNMVTPELFKAFPTAKKLSETEPEEVFEYIKSISYPNNKAKHLVGMARMLLEKFE
                     GIVPSELDDLIQLPGVGRKTANVISSVVFNKAAMAVDTHVFRVAARLGLTRNAKTPLA
                     TEKQLVKHIPEELIATAHHWLILHGRYVCTARNPKCNICGLKDVCVYYKKHPV"
     gene            complement(20111..20824)
                     /gene="dapB"
                     /locus_tag="PKU82_04120"
     CDS             complement(20111..20824)
                     /gene="dapB"
                     /locus_tag="PKU82_04120"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOZ82093.1"
                     /translation="MKIALLGYGKMGKEIETAAIESGHQIVLKINSANYNQLNKESLQ
                     TADVAIEFSKPEAAIANAKLCVDSNVPVIIGTTGWYEHKATLEQYCIQNNGTVIYASN
                     FSTGVNVFFIINEMLSKLMAPMKYDAWIEEVHHIHKKDIPSGTAITLANDVLKYNKQY
                     SGWAINDEDGKLPIYAQRIDEVTGFHSVSFRNEVDTIEITHNAFSRKGFALGAIKAAE
                     IALTKKGFYEYKNLLIEQP"
     gene            complement(20828..21424)
                     /locus_tag="PKU82_04125"
     CDS             complement(20828..21424)
                     /locus_tag="PKU82_04125"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOZ82094.1"
                     /translation="MRLRLLVVLFFTVYCVNAQQDTMIFKTRTSVIVQADSIKPTVDL
                     SQKVNKHSPHKATIYSAILPGLGQAYNKKYWKIPVIYGLTGVLTYFAIDNNKEYVVYK
                     DAYKWRLDDNSATVDKFEGIYSDEDLRILKNYYRRNRDLSFIGMGVVYLLNVVDAAVD
                     AHLFYFDVSDDLSLKVQPGIQPMSHTAFAGVKLSLQIK"
     gene            complement(21437..>22306)
                     /locus_tag="PKU82_04130"
     CDS             complement(21437..>22306)
                     /locus_tag="PKU82_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOZ82095.1"
                     /translation="LGKGLSALLDNSISVNEFKEIGVATSNAQITTLPVDRIEANPFQ
                     PRSEFKEEELLNLADSIRVHGLIQPITVRKLSADKYQIISGERRFRAAQLVGLTNVPV
                     FIRLANDQSMLEMALVENIQRENLNAIEIAISYKRLIDECKLTQEDLGLRVGKDRTTV
                     TNYLRLLKLPPQIQTALRDEKISMGHARAIISISDPVMQLKIFNEILENDLSVRSVED
                     IVRGSSKNEKKQPSISEKFIKGIEYKHIQKRLEDKFETKIEVKHRKNGSGQIMFTYFS
                     TDDLNRLLDILDA"
BASE COUNT         6892 a         4101 c         4149 g         7064 t
ORIGIN      
        1 cccgatagct atcgggaggt taaagtagga gatgaatctt ttgaaaacct gcaatgtctt
       61 gccctaaaat agagttacac aaaaaaagtg taaaacatgg gcaaaaaaat cattcaaaaa
      121 gcacagaata ttttaatgaa gaagaaaagc acaaaatcat tcaggagtta attgcaggcc
      181 gatgtactag cgtctcattc acagccttta tcaaattgtc aataacatat tgttgttgtt
      241 cttcggtgag gttgtaaaaa agcggtaaag aaatttctct ttcatagttg tcctttgctt
      301 tgggataatg ttgaagacta taacctaatt tggtataaaa cgaaaacata ggaacaggaa
      361 taaaatgtac attcactgct atatcagcat caaacatttt ttgcataatg gcatcacgtt
      421 gtttttctgt acagtttttt attctgatag gatagaggtg ataggagctt tcacgccagt
      481 ttgatttaaa ttcgggcaat tgcgcccatg gatattttgc aaatgcctca ttgtagtttg
      541 caacaatttg ttttctgcgt tctaatgttt ctgcatagcg ttcaagttct actagaccaa
      601 tggacgccat aatatctgtc atattacatt taaatccggc ttcaataata tcgtaacgcc
      661 aattgccttt ttgcattttt gccagtgcat ctttattctg tccatgcagg gtgtaaatgc
      721 acagatagtc atatatgctt tgattatcga aaggctttgg caggtttaat gcaatggcac
      781 caccttcacc tgtagttagg ttttttacag catgaaaaga aaaaacagaa acatcggtca
      841 gcgctccagt tttaatattg ttgacagtag cgccaaaaga atgtgcagca tcagacaaaa
      901 ctaaaatgcg tcctaattgc tgctgcacat cggttgcagg cacaaattta tttttgatat
      961 tttctgattg aaatatatta tttacttttt gaaaatcgca gggcagccca ccaaaatcaa
     1021 caggcataat cacctttgta tttttgttga tggcgttttc aatttcagag ggcagaatat
     1081 taaaatcatc acccacatca acaaacacag gttttgcacc acaatgaatc actacgttgg
     1141 cagtagcaga gtaggtgtat gccggtaaaa ttacttcatc accttctttt acaccaaacc
     1201 agcgaagcat tatttcaaga cctgcagtag cagaattcag ggcaagcaca ttaggcacat
     1261 tgcagtatgc tgtaagtagt ttttcaaact ttttagtctt aggccctgtt gttatccagc
     1321 ccgaaagaag tgtgtctgca acttcatcaa tgacacgttg gtccatgcga ggtggtgcaa
     1381 atggtacttt cataaattga aattaggttg taaaattacg tattgttttg ttcgggtact
     1441 gtctttaatg ctgcggcaac atattcagag ctgaaaatag catagaaaag acagaataaa
     1501 ataatgcctg cctgcctttc taagattgac tctgttagca tattcaataa aaaaatacat
     1561 tgaaatgcaa tgaaaatata gtgtctattc ctgattgcat aatagagcgg aataagcatc
     1621 attaacatta ataaagaaat tcctgtcagc ccaaaagcaa cacctgtttg tagatactga
     1681 ttatgcgggt tgaagtgatt ctgatgggca taagtaaatt tatttttcag atacatagaa
     1741 tctaaacttg acttaacatc gcccgttcct acaccaaata atggattgcg ctgaatgact
     1801 tcataagcat taatccaaag tggaattctt aacgtggtgc tgttgtattc agtttttgaa
     1861 aagtcaacat agtttgtgtt ttcttttaaa aatatttcga cctgctcgaa gcggttaatc
     1921 ttcttaaatg caaaataatc tataatcagc acagaagctg caaacaatgc tgcaagaatg
     1981 gagtatcgta ggtaatttgg tgatgaaata attctcacaa cggtataaaa caaaattgtt
     2041 gcataagata ctacggtggc aagtctgctg gcaagcaagg taatattgac taaaaagaac
     2101 aagaccaata atacccaata agcttttttg attggttttg tttgagagga agcgtcaaaa
     2161 agtatttcta agataaatag tattgcaaga tttaaataaa ttgtcagata ctgcacatgc
     2221 atacctttgg aaaacgcgac ataataaaat gcactcatgt tgccatgctg aatatattga
     2281 ttgaatgaca gcagcagatt atacaggcaa attattccgc ttcctgctat aaaaaataac
     2341 ttaaaggtct taattatatc acgcgaaatg gcattggacg agaagagtag tggaaaaata
     2401 aggaaagaac tttttgccga taattcaatc caaccggtat attgcgattg tgtgtaaagc
     2461 atgcctatac aatgcagcag aaaataacag agaggcagcc acaacaaagg gttttgaatt
     2521 ttaatctgat attttcctgt cagccattgt acaacaaaaa ataaaagcaa caggccaagc
     2581 gcataattta tccaaggctg gtcaaatggt aacaaaaacc ctgtaagcca gaataagtaa
     2641 ttaaagatta aagcccgttt ttcctgaacc attttacagt atgcaaatca aacaaaattt
     2701 tgattcggac tggtaggatt ctatacacga agataaattt tttatgaggt ttataaatta
     2761 gcggtagaat ggcctcctta acctgaataa aatactatat ttggggaaaa ttaaataaaa
     2821 tgaaaaaact aatattcaca agtttgttct ttttagggtt tttaactttt acttatgcgc
     2881 aaaaagttaa cgagaaagta caaataaatt caaaaggcgc atggtatccc ggtaaaattt
     2941 tgaaaattaa tgcagatgat cacacctatt atgtttctta tgatggttgg gatgaaggtt
     3001 ggaacgagtg ggttacgatt gatcgtttaa aagattttgc aaaagaagca cctgcacaac
     3061 cattgacaaa atttaaggtt ggagataggg ttgaagtgga atatggtatg gttcctgaac
     3121 ctgcaactgt aattgaagtt ggagaaaata aatatcatat taaatatgat aaaacagttt
     3181 ttaaagatag gtgggttaca gaaagagaga taaaaaaact ttaaacggaa atttatttta
     3241 cttgggtgat ttagaaataa gtcacccttt tttatttttg acacatgatt aaagaaatta
     3301 gagataaata tttaagagaa tttaaggaag aaacatactc ggaattttta gcttattatg
     3361 ataaccttta caacagaaag gtagaattcc gaattgcaga gtcgccagtt tttattccaa
     3421 aaaaattaaa agatttactc attgaagccg gcaatgaaat attatctcaa tcactgactc
     3481 ctgaatatct tttaaaaagt gaacaagcta ttccgcatgg attaaaagtt ccgaatgaac
     3541 ataagcatcc acattttatt gcaattgatt ttgccatctg caaagatgat aatggaaatt
     3601 atttgccgca attgatagaa ctgcaagggt ttgcctctct ttttttctgg caggatttgt
     3661 tgccgcgcac ttatgctaaa tattttcaga tacctgaagg tttcagccat ttttttgatg
     3721 gatggaatac tgaaaagcat cgtgcattga tgcgtaaagt tattcttgga aatcataaac
     3781 cggagaatgt tatcttgctt gaaatagaac ccgaaaaaca aaaaacatgg attgactttt
     3841 gggaaacaaa aacacataca ggagtagaac cggtttgtat atcgaaagtg attaaagaag
     3901 gaaaaaattt gttttatgaa ttgaatgggg taaaaactcc catttacaga atttataaca
     3961 gaattatttt tgatgagttg ctgcaacgaa aagacctgat taaagatttc aatctgactg
     4021 atgatgtaga tgtggaatgg gcaggacatc caaactggtt tttcagaatc agtaaataca
     4081 ccatgccttt tattaaaagc agatttgctt cggaatgtta ttttttaagt gatgttgaca
     4141 catcaaaatt aagccttgct gattttgtgt taaagccgct attttccttt gcaggtagtg
     4201 gggtgttgat agatgtaaca tcgtcagaca ttgaaagcat tccattggaa aaaagaaatg
     4261 aatttttaat acagcgaaaa gtaaattatg tgccttgcat ggaaacaccc gatggagctg
     4321 caaaggttga agttcgttta ctctatatct gggatgatgc tgaaaccaaa ccgcagttag
     4381 tgcttaatct tacacgatta agtaaaggtg caatgattgg tgtaagccac aataagaata
     4441 aaacctgggt aggaggaagc agctcgctga tggaaattta gtttggtgca gcagcttttt
     4501 ttctttttct gaacgattca actaagtctg aaacagtatt aaattctttt ctgtaaaaaa
     4561 caccaattcc ctgagtgtat tgtgagttaa ggttgttgat gagattgttt gtattagttt
     4621 tattaaacgc ttttgcacga agattgccgt ctttgctgat tttaaattca acctgaaagt
     4681 cgccaataat acttgaagtc tgattgctgt tgttggcaac accaacatta ctttcaacag
     4741 caactctgtt tctgaacaat tctgttgaaa tagatacatc aaactcatct ctgttaagtg
     4801 catcgcccgg acgatagtta actccgatat taaatttatc actcaactgc gatgcccaat
     4861 tcgatagctg ctgcgaaaca aattcactca aattctggcc aactgcattg ccggaaccac
     4921 tttcttcaga ggtgtttttt ccttccggtg gcgaaaaccg atttagcaca agcagactga
     4981 aaacctgcct gttcatttcg cgctcattac ctaaaatact actcagactt gttcgcacag
     5041 caggtgtggc atttggcaca tttacgtcaa aaataacatc agggctcatt aagttgttct
     5101 ttaactgcaa caatacttcc accggtactc tgctgcggtt tgaggagtca ggaatgagat
     5161 caaacgttga tgcacgaagg ttgtattttg cattgatatt gatggttgca tcgtaaggcg
     5221 agcctgccca ttgtatcagc ccgctctgaa ttttaaatct tttgttaact atattttgaa
     5281 gtgtaaacaa ataatcacct gcaagaatat tgtaatcacc atacatggaa aattcccctg
     5341 cctgcgaaat ctgcatcctt aaaattcctg atccattacc acgcatcaca tcaccaactt
     5401 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttgcaaaa gctttgccat
     5521 agaacaattc gttttgagaa gcattcgtgt taagacattg aagtttattt gcatcaactc
     5581 taaggctgaa agtgaaatca ttaaaatgat tatgaaatat ttttccatca acaatgcctt
     5641 tgtgattctg atcatcagaa attccaactt tgttaaatgc aatatatttt tcatcaaaaa
     5701 taatatcctg ctccggcaac acagaatagc gtgtgttcag atagtcaatt accatagatg
     5761 cacgttgtaa tcgggccttg ccggttaggt taggtgcttt tgccaatcct gtaagattta
     5821 actctcctgt tgcaagtcct acaattttac ttgcaaaacc attaaggtat ctttctaaag
     5881 gtgttatgct aagtttgtca actttaatat taaaactcag ctcatcattt tttggttgaa
     5941 aaatatagtc accttttaca acagcactgt ttctattgtt ttgttcaatg gcaccttcaa
     6001 tatgcaattt cttttggtct gtcagccaat caaaattcac gatggcattt cccaatggtt
     6061 cgtcattaaa cgcaaaacta tccactcgca tttcaccggt gatgattgct cgttgataga
     6121 gtgaactgaa attcaaatta ccacttgcag caccacctaa cttgatatca tacatagcca
     6181 gtaagtcatt tattaaagga accttaaatt tgaataagtt cacactcaac acatcttctt
     6241 tattgtttcc tactttgccc tgcaacataa gcgactgact tttgttggtg aacaacacct
     6301 tgtcaaatga aatagatgct gagtcgaata atatttcgtt tgccggatca acctgccatt
     6361 gttcgccttt gaactgaagc aaagactcaa gcaaatgaaa acgtgtcgct ccatttgtgt
     6421 aagtctgctc tgttgccagt ctcatttttg aggtagagag tgaatcgttg gcaactgtaa
     6481 gtacaatgga tgatttgctt ttttgtgtct ctgcatcaag taaaatattt ttcagaatta
     6541 aactgtcaga aattttaacc tgagaggcag ataaatcaat atttaataaa ttgttcttat
     6601 tgtttgatgt gagtttaatg tttttatttt cgaaattttt gtaattaatc cagggtgatg
     6661 ttgcatccac ttctatttcg caatgctgac gattcagcct tcctgaaaac tcagtttgat
     6721 tacttacaga caatcccgga gtaaagatgt tcaacacagg atttacatct tttatatttc
     6781 cactgaatga aaaaatagta ttagtttttt ctacatcagg tgataattta agcatgaatg
     6841 gtggcagatg gcagttaagt agatgaccga atgaataaac aattttagat aatatatact
     6901 gaccattaat attcaaatcc agtaaatcgg aattcacatt caggtttctg ttattattct
     6961 ccatgcccga ctgaatcaat attttttcta caaaagcaga tttatattct tctgaataac
     7021 taatattttt aagttgcagt tttccctcaa actcatcaaa attaaacccg cgcatgttca
     7081 gatttgcttc agtagaaagt agggcagatg tatctctttt tagcagatta agtttagtca
     7141 atgcggcatt tttaactata gcattaaaat caaacaaagg cagtttctct ctgaaatcaa
     7201 tacttccttt aaatgaaacc tgtgcatttc tgtcattcat gtttaatgtt ccgttaaaaa
     7261 gtttccgggc cacatcagca tctaagttga tgtttgaata atcatagcca tttaaattaa
     7321 catgacttac caaacctgtc agacgtgcat taactttctc tgatgtaaat cctgtaccat
     7381 ttagctgtgc attaaaactg attgttccta cggcatttcc caatcgccag aatttaccaa
     7441 tatcaaagtt gtcggcagtg agtttaccgg agtaagttga ctcatttgcc ttgtttttga
     7501 attttaaatt tacatcggtt gacaggttgc cgattgctgt tgtaatattt ccatacgaaa
     7561 caaagtcgtt ataaaatcct gtaaaaacac ctctgtaaac aatcttacca agttcagaaa
     7621 tgttatcagg caatttaata tattgttgct ctgtaaatgg atattgttta atggtttcaa
     7681 tatcggtctt gttggttgaa agatgtttta tgtttagatt tataaaagtt gtatttacat
     7741 caggcaaacc attaaggctt acatcacctt caaatcgtgt gctctttcca taagcaattt
     7801 taagtttttt ggtttttaga ttgctcaccg ttcctctggc agtaccacta aactcaacac
     7861 gtttatctag gccaaaaagt tcttcggcaa agtactgaat gtcgtttgaa gaaattgtgc
     7921 tttcattaaa ctcgcctttc atcattacct tattgatgta gtcttcaaag tctgtcatac
     7981 tgttatacga catactgtag tgtccggcat caatagaaga gtagggtgtg cgcaacagaa
     8041 ttttattaaa cgacatgctg tcgttataaa aagcagcata agtagaaaaa gagtttattt
     8101 taaatcctga tttttctttc agagatattt tacttccata aaatgaaaac gagctgtctg
     8161 ttggctttat attgtaaagt gttccattaa agtctgacac gtcaatatct tcaaaatcaa
     8221 tacaaggtgt tttatcatcc cacttaaaat cgcgataagt gaaatgtata ttgcttaaaa
     8281 taaccctatt cacggcaaaa taaaatggtg atgatgtagt gtcttttgaa ggagaactga
     8341 aataatctat aataaaatcg aagttcatat ctcttgtgct cttcgacttt gataatgcta
     8401 ttcgcgcttt gtccaactgc acttcatcaa gaatcagttt tttattcctg ataaacaagg
     8461 actgaaaagc cactgttgct gtaaggtgtt tggcatacaa cagcgtatct tgctgtttgt
     8521 cggcaataaa cacatcatca agatatacac tttttatcaa tacaaacctt accccgccaa
     8581 tatgtactgt tgtatttaat tcggatgata gatatcctgt tacacgatca acaacccaac
     8641 tttgaacttt ttgaaatcgt aagctgacag aaaacaaaaa aagcagcagc aacaatgtgc
     8701 caattgtaat aagtaatatt tttaaaaatt tcctgatgac ctgttgtttt tgaagcggat
     8761 aaaaatagtg taattattta agtggattaa ccaatgttgc cttaaaaata atgccacact
     8821 cttgttagtt taccctaaat tcattgcatt atagatggtt aataagatgt gttttgaagc
     8881 ctgtcagaca aactgatttt tcctcttaac tttgctgact gatttttagt gtaaaagaag
     8941 gtgacggtta ctgataaaaa agacataatc atacttggca ttgaatcatc ctgtgatgaa
     9001 acttctgctg ccgtaagtcg caatacacag attttatcaa atgttattgc caaccaaacc
     9061 gtacatcaga aatatggcgg tgtggttcct gaattagcca gcagggcaca tcagtcaaac
     9121 attgttcctg tagtggatca ggcattacga gaagcggcaa taaatatcag cgatgttgat
     9181 gccattgcct ttactcgtgg accaggactt attggctcac taatggttgg tgtttctttt
     9241 gccaagtctg ctgctgctgc attaaacaag ccgctcatag aagtcaacca catgcaggca
     9301 catgttcttg ctaacttttt aagtttagaa gggcaggagg gcaaaactcc gtcatttcct
     9361 tttttatgcc tcacagtttc gggtggccat acacaaattg taagagtaac ttcaccacag
     9421 caaatggaaa taattgggca gacactcgat gatgcagcag gtgaggcatt tgataaaatt
     9481 gccaaagtat ttgagttgcc ctatcccgga gggccgctca ttgacagata tgcacaaagt
     9541 ggcaatccaa aagcatttaa ttttcctgtg gcaaacctgc cggggctcaa ttattccttt
     9601 agtggactga aaacttctgt cttgtatttt ttaaagaaac aacttgcgca aaatcctgct
     9661 tttattgctg aaaacagaga tgatttatgt gcttccgtgc aatataccat tgtaaaaaca
     9721 ctcatactta aattaaaaaa ggcagcttta gaaacaggca tcagcaatat tgccattgct
     9781 ggcggagtat cagcaaacag cggactacgg aaagccgtaa cagaagaggc agttaaaaat
     9841 aattggcagg tacatattcc gccactttcg ctgtgtactg acaatggcgg tatgatttgt
     9901 cttgctgcat atttcaaata tcttgctgct gatttttcaa cacttgatgt ggccccaagt
     9961 gcccgtttcg ggttctgact ttatgcccat ttgttgacat tgtacctgtt caaaaccttg
    10021 acatacagga gttgtttact ttaaaataca tattaccttt gttaattaat ctataaaaat
    10081 attatcatgg aaaaccagag taaagggaat aatcgtattt ggattgtttt atttgctgct
    10141 tcggcattgt ttaacgtata tcaatggcgc aatcacacat caactacaga ggtatttgag
    10201 gttactaaag acagcctcat tacagcacgt gttgacgttg aaaaagaatt gggtgccacc
    10261 atcgaagagc taaacaagta caaaggtatc aatgacaatc tggacagtct tttaaccgag
    10321 gccaatcata aaattgatga acagaaagag cgcattcaga aattaataag aagcgaaggc
    10381 aacagcgcag cattaaacaa aaagctgatg gcagaattag agtcactcaa aaaactgcgt
    10441 gatgaatatt tggataagat agataccctg atggtagaaa atcaaaaact caaagatgat
    10501 aatgcacaat tatcaggcac tgtcgaaacg ctttctaaaa atttagaaac aacggttaac
    10561 actgcatcgg ttttaaagtc agaatatttt aaagtttcta ctttcaagaa aaaaggcaat
    10621 ggcaaatttg tgtccaccgc tttatcaaaa cgtaccaata aaatggatgt gtgtttttct
    10681 gttcttgaaa ataaaattgc caaagcagga gaaaaaaatg tttatctcag aatcattgag
    10741 ccgggtggta aggttatggg taaccgctct gccggatcta catcttttaa aatggatagt
    10801 ggtgaagagg taatgtgtac tgttgtttca tcgctgcgct acgaaaatgc caccgttgac
    10861 aattgcctta gttatgagga gaacgataga atatttacac cgggcaccta tctgatagaa
    10921 gtgtatattg atggcaacct cagcggggca tcatcttatg tgttgagata agtttttgaa
    10981 ttctaccaaa ggctttagcc tgagatagat gatccctaag cctcatatcg gactttagtc
    11041 cacaaataaa aaatccaccg ctgttaaaaa ccatcggtgg attttttttg ggggattggc
    11101 ttttctatcc atcaggctaa agtcttatgc taatatctat tgtccttaca cctaatcacc
    11161 tctgtcagcc gtggctaatc acaatttaat gcctgatgtt attttcactt ttttcaattc
    11221 tgaattctgc actctgaatt ctgaattttc actttacttc atcacgtttc acataatcac
    11281 ttaatcaccc cttgtcagtc gttgttgatc ataacccctc acttcatcgc gcttcactta
    11341 atcactttct cctgattgca taaatcaact catcttcata acgattgttt ttataaatga
    11401 ttttttcaag gcgggcttcc aaaacaaatc ctgttttttc caatacgcgt tgcgaagctt
    11461 tattgctgcc aaaggggcgt gcaaaaattc tatctatttc aaaactgctg aaaccatatt
    11521 caactatttt tttaatagca atggaaacaa atcctttacc ccaatatttt tcagcaagcc
    11581 aatatccaag ctctgcattt tttaaaaaaa tgtcagtctg cgggtgtagt ccaatagcac
    11641 ctattgcctc tccatctaag tcaattgcaa atacctgagc aggtttaaaa ccgttaactc
    11701 ttctgataaa ttccagtgca tgtgctttcg tatatggaaa gggaaactga ttggtcatgt
    11761 tatctgcaat tgcactgttg ttggcatgca aagcaaatat ttctgcatcg tcaatttgca
    11821 aactacgcaa tacaatttct gacatcagca attattttac aatagctatt tgttttacta
    11881 tgttaaacga gtttgaagta tagcggatat tgtaaatgtc ttttgcaaga gtagaaacct
    11941 gtgttgcttg tttatgatag cctgcatcaa gtgtccattg ttcttccata accaattgtc
    12001 cgatggtatt aaataccttc atggagagtt gtgttttttc aggaagatat atgtcagaaa
    12061 caaaaacatc ttttgccgga ttgggcatca ggttgatatt tactgttgca ggactttctg
    12121 tgttacacga aactaaaata gccggcaata cattagctct tccatcaaaa tcatattgca
    12181 ccaatcggaa aaatgtgtgt tcagtttttt gatcaatggt aaaagaataa aaattttgtt
    12241 tgatggtggt accattacct tcaacacgac ctatagtaac aaaatcattt ccatcggctg
    12301 attgttgcaa ttcaaaataa tgattgttta tttcagatgc agttgtccaa ttcagattaa
    12361 caatgttgtt cttacagtta ccggtaaacg aaagtaattc aacaggcagt ggatttgatt
    12421 ctggtgcaat attgaaattt tctgtattac tgaaattatt ccagttggta cgcagcaccg
    12481 atcctaacac aggtgaagcg cccggagtta cagcaccgcc aatatctttc caccatacag
    12541 gaggggcagc ctgtccccac tgacaaagtt tgagtaatgg ccatgaagca atggcatcct
    12601 gtgcatcatc ggcataaagc gaaaactccg ctaacgaact accggaaact ctgtttattt
    12661 tctgatacca tcctgcattt actgcaccta aggtgggttc acgtacagca cggttaaaac
    12721 catcagtatt cgggtcatta ttaaccatac gtacagcaaa agtattgggt gttgcactca
    12781 atggctttat ttcaactgga cgaaaacgtg cgggaggtat tgaagaacct acaggaaata
    12841 aatacacaga tgtagtgttg gtgtttcgcc acaacttacc actgcctgtg ctactcacaa
    12901 aaccctgaac aggagttgag ttaagtccac ctgtacgcgt aatagctgcc ggtgctgtat
    12961 tggtaacatg catcacattt ccttgtgtgg caagttctct gtcgttcaac ttcagcaatc
    13021 cctgaaccgt tgcatcaata ttttgggttt ttatacctgt gcctgtcaga tttaaggtaa
    13081 agaagaaagt aggtgtacta cctcttatag tttgattggc gccaaacagt tccacttcac
    13141 ccgggctgct gttgataaaa gcatttccag atgaattgtt tgtccagtcg ccttgtatac
    13201 gcacctgacc ggagttatcc catgtaccaa tgttgctcag gttatcctga ttaataacac
    13261 caccttcaac atgcatcatt gcagtaggtt gaacaaaaat cagtgacccg tcattgacaa
    13321 taagtcctgt tccaggtgac ggacatgtaa caacattgat gtaatttgtt ctagtaaaag
    13381 tgttgttgcc aaaagcattt gttgcagtta atgaaacagt gtaggtgcct gcagtagcat
    13441 agctaataac aggattctgc aaggcagatg ctgcaggtgc accaccggta aaagtccatg
    13501 cccatgatga tggtgagttg gttgactgat ctacataagt cacagcctga ccttcgcaga
    13561 tggttgtagg aaatcctgaa aaagatgcaa caggtgcaga gcaaagattt accgtaatat
    13621 atcctgatac agtttttgtg ttcgagccac caccattagt tgcaattaat gtaactgcgt
    13681 aagtgccggg tgtgttatac gttattgttg gattttgtgc tacagatgat aacggtgtgc
    13741 caccggcaaa tgtccacgac catgatgtgg gtgtgttagt tgaaagatcg gtgaagttta
    13801 ctgtaccacc ggaacacagt gttgttggcg atgcactaaa atcagcgacc ggtggagcag
    13861 ggcaattgtt tactgttatg taatttgttt ttgttaatgt gttgctgcca taggcattag
    13921 ttgaggttaa tgtaacatta taagttcctg cagtggcata agtaatcaca gggttttgtg
    13981 cagtagatgt tcccggtgta cctcccggaa aagtccaact ccagcttgtt ggtgtgttgg
    14041 tactcaaatc tacaaactgt acagtatttc cctgacaaac ggttgttgga ttagctgtaa
    14101 aatcaggtgt aggtggaggg caggttgcaa tctgtacata atttgtttta gtaagtgtat
    14161 tgcttccaaa tgcattgctt acagttaggg taactgcgta gttgcccggt gcggcataaa
    14221 aaattacagg aggattctgt cctacaaaag tatttggtac ccctcctgga aaagaccacg
    14281 tccacgatgt tggagtattg atggatgcat ctgtaaaagt tacgttcagc gtattgcatc
    14341 ctgtcagtgg tgtaccatta aaatcagcaa taggtgcatt gatggcacag cttcccgcag
    14401 aaagcgcaac catagctgtt ggtccgcagg cattagtcac cacactacga ctcattatat
    14461 catttccacc ggcagtttca ctgattggtg taagtgtacg tctgtctgta tttgggccaa
    14521 tagcataatt cattactgtc acaggcacaa tagtgtgacc taactgatga ctgtgtccta
    14581 attcatgcaa acaaactgat tcaaaatcga aattgccacc accggttgca gcagggccat
    14641 agttccagtt tatgcctccg gttccattgg tacgaaactt taaatcgttt tcgttaagat
    14701 accaaacgcc tgttgcgcat gcactgtaat aactgtacga aattccgaga actccggcag
    14761 gcaatgcaca tgaaccatca aaggtcacta agttagttcc gtctaaagcc tgacaagttg
    14821 ttgcagtagt acccgagcgg ttgaagttta caaaagttcc acagcgccat gtttgtaatg
    14881 ctctttcaaa tgcagcaaca gcagcagcat taccattaaa tgttgtgttg taacgaaaag
    14941 tataacctcc ggttccatta ttgttaatca agtcgggttg ataataatta ctaccactaa
    15001 caacatttgt ttcattataa ttaatggtaa ttgaaattgc agaagttgta gtttctccaa
    15061 gattgtttgt tactcttatt aatccgcttc caccttgtgt aggtacccaa acctgaattt
    15121 gtgtgttgct ccacgactga atatgatttt gcggagcaga aatataactt acacctccag
    15181 tattggcatc aggaaattca acgtttgaag ttccgttaac ataggtggca ccaaaattat
    15241 ttccgtttat tgtgattgtt gaaaatgttc ctgccgtagt tgttgccggt actattgaag
    15301 caatagttgg cggaatcatt attttattta cagatggagg tgcctgctgt ggtgcaatag
    15361 aagaaatgcg ctgaacgcca cctgtagcat tgataatctg atttcgtaat gcattgatgt
    15421 cactgtagtt ttcaaacgga cctgttgctg aattacttgt ttcatcaaac aaaattaaac
    15481 tctgactcaa agcatatcct tcaaacattc ctgacagttt acttcctgac ggctggctgt
    15541 gtggaattag aaaaaatatt ccattctgtc cgggtttaat attcaaagat ggttctgact
    15601 taatcatgat gttaccaaca ataccgcctt ctgtaatcaa tgcaatctga ctgccctgaa
    15661 ttgtgccttt ataaactgca gaaattgaaa gtgtagtgga agtgtagatg aaatcattac
    15721 tgttgttcca gtagctgttt tgttgcaagg cagtagcttc aacaacaaca gttgcattac
    15781 tgattctgtc ctgtaaagaa acaggtgtca tcatacactg cgaccatgct gttacaggta
    15841 gcagaaataa taaaatatag taaacaaact ttttcatact catatagttt ttaatgatga
    15901 aatgagcttg ttatttagaa tcgttttgtt gtgtaaaatc tcttgattta attaacggtg
    15961 catctttcga tttttcgttt acagcagttt tttgaggaat aggtttgtaa taaattcctg
    16021 ctgactccgg ttgtccatac aatgatggaa ctaaatattt tcctgcagcg tctcctgttt
    16081 ttggaagttc aaccaaacga ctatctggat tttgttgcag ataaggatcg tttctttcag
    16141 ccgttagtgt ccagcttacc ttcattccgg caacgccccc ggcaattacg aagttattac
    16201 ctgtaacttc cgatttaaca taaagttgtg caggtgcacc aataggagtg aggttgtagt
    16261 tgtagtttga gttatttata ctattgaaat aagaaggtag ttgaatagtt gcctcaccat
    16321 ttgcatcgag tacaacatta cctctgtaat aattaagtac ttcatttgat tcaatagaaa
    16381 aatgtttcag aaattttcct gatggatcta gtgggtgatc aattacaaaa gtttttgttc
    16441 ctgaagcggc taaatcaccg ttagcaaata ttgcataacg tgtaggacca ttgggtgcag
    16501 caacttcttg tcctaaaata ccaattgcat tggcaccaac tgaattatta cttccaataa
    16561 cagcaattga ggtagcattt gttcctgaaa cggttgttac atctccccat actgcatagg
    16621 gtgtggggcc catccctgcc tgacccatta caccggttcc actattagaa ctacctatac
    16681 ctgtaacgcc attattgcct ccaaaaaatg aaacacctat aacaccggat ccatatgcag
    16741 aatcaattcc agttacaccg gcaccaccga gataacttgt tatgattgtt tttctattac
    16801 ttccccaaac tgcactaccg gcaacagcag cagaactgat gtttgaaaaa ttaataatgc
    16861 cgaaattggt gcctcggaaa gaaccagcag aaccatttac taaatatgtt cctggaagat
    16921 tatttcctgt aacaagtaat cctgttccat tggcattaga attctgaaac attcctgcaa
    16981 atgctgtatt acctccggaa aatcctacag ttccataacc ggaagatgaa ctgccccaaa
    17041 cgccaacacc ggttcctgtg ttagcaccag atactccaat gcctgtcgtt gcagagtaac
    17101 catttagtgc aaatgcacca attgggctca atgcattgtt gataccatta ctacttcctg
    17161 ttgcataaac agaaaacaca tcaccgttat tattacctac agaagtatta ttaactataa
    17221 tttctcctcc tgaacgaatg cgcattcttt catttgcagc agcgttgcca tttgttttaa
    17281 ataatatatg tgtgttggtt aatgtgccga gaaaattttt tgtagtatct attgaactat
    17341 tcccaagtgt tagccatgct gttgaatcaa gtaaaccact tgaactcaca tttagtgtac
    17401 ctgtagggtc tgccatcaca aaacgattac caacaccagt aagtgttgat gatctaattt
    17461 tcccaatcac atctaatttt tgcataggaa cattggtgcc aattcctaca ttttgtgcct
    17521 gatttgtgct tgctatcaga agaatagtca atacagtgaa aagttttttc attatcattc
    17581 aatttagagt aacttcaaag atatgttaag gaaatcataa aacaaagatt tttgtagaaa
    17641 aatatcggca aaagggtctt gtaacgattt cgtcattttc tccattactg acaaaaatca
    17701 caccaagttg cactctcaaa agggatactt tcgcgacaaa atatcttaaa attatgaaac
    17761 gatttaagta cctgttaatt ctgttgcctt ttagcttaaa ctttgcatta gcccaagatg
    17821 ttgatactat tgagaataaa catcttataa acattaaaca attgactttt ggaggtgata
    17881 atgccgaggc ttattttagt tttgacaaca ataaattagc ttttcaatct aacaataaag
    17941 catggggtgt tgaatgcgat cagatatttt tattagatct taataactgg aaatccgata
    18001 gcaaacctcc attaatcagt accggtaaag gccgtaccac ctgctcatat tttatgcccg
    18061 gtaataaaac cattctttat gcaagcacgc atttaagagg agataaatgt cccgaaacag
    18121 gtgaccttag acgtggtgga aaatatttat ggccgatttt taatacctac gatatttttg
    18181 ttgctgacct gaatggaaaa atagtgaaac aacttaccaa tgcaccggga tatgatgctg
    18241 aagcaacagt ttcgcccgat ggtaaaaaga ttgtttttac tagcgatcgt acaggtgatc
    18301 ttgaattgtg gacaatggat attgatggaa gcaatcagaa gcaaattacg tttggtttgg
    18361 gttatgatgg gggtgcattt ttctcacccg acagcaaaca attggtgttc cgctcatcaa
    18421 ggccaactac agctgaagaa attcaagagt acaaacagtt gttaagcgaa ggtttggttg
    18481 caccaaccaa tatggaaatc tatacttgta atgttgatgg tagcaatctg aaacagatta
    18541 ctcatcttgg tagagcaaac tgggcacctt tctttcatcc atcaggaaag aaaattcttt
    18601 tctcatccaa tcatgaatcg aaatcaggat ttaattttca gttgtatatg attaatactg
    18661 atggaacagg tttagtacaa attaccgatc agagtgtgtt taatgcattt ccgatgtttt
    18721 cgcctgatgg caagaaatta attttttcaa gtaacagaaa taatggtgga acacatgaca
    18781 ccaatttgtt tattgctgac tggaaagatt aaccttattg ttgttttttt gttgtcagta
    18841 aaacaaaagt aaaatgttga tagaaattaa cgagaataat attgatgaca gaaaaatcaa
    18901 gaaggttgtt gaatgtatca acaatgatgg catcatcatt ataccaaccg atacagttta
    18961 tgctttggca tgcagtattt acagtttgcc tgccattgag aaagtaagtc gttttaaagg
    19021 tgtaaaacct gaaaaggcta atctttcttt tttgtgtaat gatttaaaaa acattgcaga
    19081 atacaccaaa cctttcgacc gtagtgtata taaattattg aaccgttcat tgcccgggcc
    19141 atttactttc atacttgaag catcaacaaa agtgcccggt atattcaggg caagaaaaaa
    19201 gaatatcggt attcgtattc ctgatcagaa agtaactcta tcaatcattc agcaactggg
    19261 gcatccactg atgtcggctt cactgcatga tgtaaatgat ccaattacag attatctaac
    19321 cgaccctgat gaaatttatg aaaggttcag cgatatggtt gatatgattg ttgattgtgg
    19381 agcaggaggg aacgtgccga gtacaattgt agattgtaca acagatgaaa ttactgttgt
    19441 gcgcgaaggc aaaggagtgt tatcttaaac aggatgtttt ttataataaa cacaaacatc
    19501 ttttaatccg caaatattac attttggatt tcgagccgta caaacataac ggccatgaag
    19561 tatgagccaa tggtgtgcag ttgcaattag ttcttccggt atatgtttta ctaattgctt
    19621 ttctgtagct aatggtgttt ttgcattgcg tgtcagacct aatcgtgctg ccacacgaaa
    19681 aacgtgtgtg tcaacagcca ttgcggcttt attgaaaaca acagatgaaa ttacattagc
    19741 agtttttctt ccaacaccgg gcaactgaat aagatcatca agttctgatg gaacaatgcc
    19801 ttcaaacttt tctaaaagca ttcgcgccat gccgacaaga tgttttgcct tgttatttgg
    19861 atagcttata gattttatat actcaaaaac ttcttcaggc tctgtttctg aaagtttttt
    19921 agccgtagga aaagctttga ataattcagg agtaaccata ttgactcttt tgtcagtaca
    19981 ttgagctgac agaataactg caactaaaag ttgatatgga tttttgtaaa ccaattcagt
    20041 ttctgccaca ggcatgtttt tcctgaagta accgattaca tatttatatc gctcttttag
    20101 caacataaaa tcaaggctgt tctattaaaa ggttcttata ctcgtaaaat cctttttttg
    20161 tcaaagctat ttctgcagct tttatggcac caagtgcaaa tccttttctg ctgaatgcat
    20221 tgtgtgtaat ctcaatggtg tcaacttcgt tacggaatga aactgaatga aaacctgtta
    20281 cttcgtcaat gcgctgtgcg tatatgggaa gtttaccatc ttcatcatta attgcccatc
    20341 ctgaatattg tttgttatat ttcagtacat cattagctag tgtaatggct gtgccacttg
    20401 gaatatcttt tttatgaatg tgatgcactt cttcaatcca tgcatcatat ttcataggcg
    20461 ccataagttt agacaacatc tcattaatga taaaaaaaac atttacaccc gtgctgaagt
    20521 tagaagcata aataaccgtt ccattatttt gaatacaata ttgctctaat gttgctttgt
    20581 gttcatacca acctgttgta cctataatta caggaacgtt tgaatcaaca cataatttag
    20641 cattggctat agcagcttca ggctttgaaa attctatggc aacatcggca gtttgtagag
    20701 actccttatt aagctgattg taattggcag agttaatttt tagaacaatt tgatgtccac
    20761 tctcaatagc agcagtttca atttccttgc ccatttttcc gtatcctaac agtgcaattt
    20821 tcatatctta ctttatttgt aaactcagtt ttacgcctgc aaatgcagta tggctcattg
    20881 gttgaatgcc gggctgcact tttaatgata aatcatcgct tacatcaaaa taaaacaagt
    20941 gagcatcaac tgctgcatca actacattga gcaaataaac aacacccatt ccgataaagg
    21001 aaaggtcgcg gtttctgcga taatagtttt ttaaaattcg caaatcctca tctgagtaaa
    21061 taccttcaaa cttatctaca gtagcactgt tgtcatcgag ccgccattta taggcatctt
    21121 tataaactac atactctttg ttgttgtcaa tagcaaaata ggtaagcact cctgttagtc
    21181 cgtaaattac aggtatcttc cagtattttt tattgtaagc ctgacctaag cctggtaaaa
    21241 tagctgaata gattgtagcc ttatgtggag aatgtttatt gactttttga gataaatcaa
    21301 cagtgggttt tatggaatcg gcctgaacaa taacacttgt tctggtctta aaaatcatgg
    21361 tgtcctgttg ggcatttacg cagtaaaccg taaaaaacag aactaccaat aatcttaacc
    21421 tcatgcagct aaaaatttat gcgtcaagaa tatcgagtaa ccgatttaaa tcgtcagtag
    21481 aaaaataggt aaacataatt tgccccgaac catttttacg atgtttaact tcaatcttcg
    21541 tttcaaactt atcttccaaa cgtttctgaa tatgtttgta ctctattcct ttgataaact
    21601 tttcgctgat gcttggctgc ttcttctcat tttttgatga gcctcgtaca atatcttcaa
    21661 cagagcgaac gcttaaatca ttttcaagta tttcattgaa aattttcaac tgcataacag
    21721 gatcgctaat gctaattatg gcacgtgcat ggcccatact tattttttca tcacgaagag
    21781 cagtttgaat ttgtggtggc aactttaaca aacgaagata gtttgttact gtcgtacggt
    21841 ctttgcctac acgaagtcca aggtcttctt gcgttagttt acattcatca atgagtcttt
    21901 tgtagcttat ggcaatttca atagcattta agttttctcg ctgaatattt tccaccaatg
    21961 ccatttccag catactctga tcgttggcaa ggcggataaa aacaggaaca tttgttaagc
    22021 caactaattg tgctgcacga aatcttcttt caccggagat tatctgatac ttatcggcag
    22081 aaagttttct tacggtaata ggttgaatga gtccatgtac tctgatagag tcggcaaggt
    22141 ttaataattc ttcttcttta aactcgctgc gtggctgaaa tgggttggct tcaatacgat
    22201 caacaggcag ggttgtgatt tgcgcattag atgtagcaac accaatctct ttaaattcat
    22261 ttaccgatat gctgttgtca agcaaggcac ttagaccttt gccaag
//
LOCUS       DAOOOL010000030        22278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_180_length_22278_cov_164.949107, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000030 DAOOOL010000000
VERSION     DAOOOL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_22278_cov_164.949107"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKU82_04135"
     CDS             <1..188
                     /locus_tag="PKU82_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82096.1"
                     /translation="TAMPNADAIINTSTLAYRNGEINYLEYAAALENATAIRTGFLQS
                     IHDVNQTVININSLTNK"
     gene            200..1462
                     /locus_tag="PKU82_04140"
     CDS             200..1462
                     /locus_tag="PKU82_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ82097.1"
                     /translation="MQTIKYYLYIILISLLTGSCSTKNTTGEAENPVDAHAHESATTT
                     TLTSEQIKSIGVTTGGFEKKQLTASLKANGILKVPNQNRANATSLLGGTVKEILVQTG
                     ASVQKGQTIAIISNNQFISMQEEYLSISARAGLAEADFERQKTLKEGNAASLKAFQQA
                     EAELKSLRAKKSGLQKQLSLIGIDAQQLTPENIQSSVNIKSPINGSVSNVLVNIGSYL
                     EASNPVAEIVDNSQLHLDLYVYEKDLNKVKVGQTIHFTLTNNPGKEYDADIYAISNTF
                     EPNTKAVSVHATVKGDKHGLIDGMSITALISLENATYLAVPTEAIVSNEGQDFIFIVS
                     DKEVPPQEAAAHDHSAHDHAAHDHSAEPVTESGTVFERIPIRKGTTDVGYSEITLLKD
                     IPADSKVVTKGAFFILAKMTNQGEAHEH"
     gene            1544..2023
                     /locus_tag="PKU82_04145"
     CDS             1544..2023
                     /locus_tag="PKU82_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82098.1"
                     /translation="MRNIKSNIKNIFIATIIGFMLHGCSSKPSETVNSTKVSKAVNDE
                     PVNEPAEPIELDNGQKWKVNEEMKPFLTNGKNLLNEYIQNEDNDYTGLAAALSEQDNK
                     LIESCTMDGKSHEELHKWLHPHLDMVADLESTKDTAQAKKLVEQLKASYVDFEKYFQ"
     gene            2179..4335
                     /locus_tag="PKU82_04150"
     CDS             2179..4335
                     /locus_tag="PKU82_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ82099.1"
                     /translation="MTEFFNQLLHEIERPLTNPVLIFSLILFIILLSPILLRRLNIPG
                     IIGLIIAGVIIGPHGLYILEKNSAINLFSTIGLLYIMFIAGLELDLNEFKSHRNKSLV
                     FGFFTFIIPLGIGFPVCHYLLYYDFNASLLTASMFATHTLVAYPIVSKFGVSKNQAVA
                     ITVGGTILTDTAVLIILAVIMGNSQGNLNQEFWIRLGASLIIFSAIMFIVIPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFAAFLAEVAGVEPIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVSVIMNGPTALIIAGTLTIVAITGKWLASFLTQVIFK
                     YTSSQRQLIFGLSSAHAAATLAVILVGYKAGILDENILNGTIILILITCIIASFATER
                     AAKKIIVETEDDSSDLLKSSSASNEHILLPVANVGKMEKLLDFAILIKDKKSVNPVSV
                     LSVVSDNSEAEKNILKAKNKLEDFVKQASGTETKVNVITTIDHNAASGITRCSREIMA
                     DIIVLGWPQRTGFLDKLIGEKIESILNNTDKTTLICFLEKPLELHKRIVVAVPPLAEH
                     ENGFGLWLTKLAKLAQELSIPIHTYCNEATEKSIRKFFSKEKLAASLSVSLFSDWDDF
                     LVLARNIHADDFVVLVSARRGATSYMSILDGLPLKIEKHFSDNSRVIIYPQQYDGNIN
                     LENYSEVSTETLNRGIEVVQKIGKEIGSMLKKKNQE"
     gene            complement(4352..4858)
                     /locus_tag="PKU82_04155"
     CDS             complement(4352..4858)
                     /locus_tag="PKU82_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HOZ82100.1"
                     /translation="MEQEIKNRVADSGILQIDPKDFIPATTITELDIKGWLFEEMILK
                     EKDFREHIRQHNWQQYDGVAVAVYCSVDAIIPTWAFMLVSEALQPHTTNIFFGNATQL
                     KEKLTIDTIEKINIQNYNNKRIVIKGCGDENFSPAVYMLLTQKLKPVVKSIMYGEPCS
                     TVPVYKSK"
     gene            complement(4861..5175)
                     /locus_tag="PKU82_04160"
     CDS             complement(4861..5175)
                     /locus_tag="PKU82_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOZ82101.1"
                     /translation="MEEPKELYARVIETIKTCYDPEIPVDIYELGLIYEVKIIEGNDV
                     HVKMTLTSPACPVAESLPLEVEQKIKSIPDVNDAKVELTFDPPWDKDMMSEEARMELG
                     MW"
     gene            5338..5742
                     /locus_tag="PKU82_04165"
     CDS             5338..5742
                     /locus_tag="PKU82_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ82102.1"
                     /translation="MKQILIVLLTIGLSFSSSAQQKTVLEANEKVAIISFEKIEHNFG
                     KIKEGDKAEHVFLFKNTGNTPLIVTDVSVTCGCTAAEKPDAPIMPGKDGKIKIVFNSA
                     GKVGSQNRTITVSTNAKNKITYLKMIGEVSEK"
     gene            complement(5758..6847)
                     /gene="prfB"
                     /locus_tag="PKU82_04170"
     CDS             complement(join(5758..6786,6788..6847))
                     /gene="prfB"
                     /locus_tag="PKU82_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106623597.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOZ82103.1"
                     /translation="MTQEQLTECKERVDALRGHLDIDVKLLKIKEEEQLTHAPDFWNN
                     PTRAEQILKSIKLKKNWTDTFAAVQQAVDDLGVLFDFSKTGDASEQEVEEQYTSVQKS
                     LEELEFKNMLSGEEDALSAVLTINAGAGGTESCDWASMLMRMYIRWAERMNYRINEID
                     RQDGDVAGIKSVTLEIEGDFAYGYLKSENGVHRLVRISPFDSNAKRHTSFASVFAYPL
                     IDDTIEIEIKDADIEMQTSRSGGAGGQNVNKVETKVQLKHLPTGIVVVCQVERSQSGN
                     RERAMQMLRSQLYELELRKRNEAKAATEAGKKKIEWGSQIRSYVFHPYKMVKDLRTDC
                     ETSNVQAVMDGELHEFMKAFLMEFGERV"
     gene            complement(6892..7266)
                     /locus_tag="PKU82_04175"
     CDS             complement(6892..7266)
                     /locus_tag="PKU82_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82104.1"
                     /translation="MNKGFITYILQLAGITLLYILLYYLMKDFLAARFYPSWWKCGIF
                     FFVLFSVIHYLRLTKSKGNESIRFFMASTAFKLFLMLAIIVGYIFLNQTMAVPFVISF
                     CLAYFLFMIFDVAKAYTLMKTT"
     gene            complement(7453..7872)
                     /locus_tag="PKU82_04180"
     CDS             complement(7453..7872)
                     /locus_tag="PKU82_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOZ82105.1"
                     /translation="MKNKTTTVKPGETASGSINLIAAGTSIEGEIKSNGDLRIDGHIV
                     GSVFSKAKVVVGTTGVVDGDIVCQNADVSGTVKGTSTVTEMLFLKSTAKINGDINTGK
                     LVVEVGASFTGNCNMGPMVKDIKNVERSEAKLQEKSA"
     gene            complement(7950..10625)
                     /locus_tag="PKU82_04185"
     CDS             complement(7950..10625)
                     /locus_tag="PKU82_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82106.1"
                     /translation="MKSSNHVKYFIILTLFLSACSTQKNTVINRAYHNLTAHYNGYFN
                     ARERVKEGTKTLSALHVDHYDRVLDVFKYATDDKAKTIYPDMDEAIKKSSIVIQRHSM
                     FINGKERCRWIPDNYMIIGKAQFLKHDFWTALETFQYVASTYKDNPKRFDGLIWQMQT
                     QMQLGKMADAEYMLDYLKNEKQLPSKYKPLFAAVAADFYLKKENYPKAIEQLTKAADL
                     ATKRADKIRYSYILAQIYQKVDSNDKAFAIYRKVIKMNPEYEMAFNAKINRARSFDVN
                     SADAQQVKDELNKMLKDEKNKDYLDQIYYALSGISMREKDTTEAFQFLKKSVAASTNN
                     ANQKGISYLEMGEISFRKLDFRAAQLYYDSAATNITSDYPDYALIQNKKTNLSRLIKN
                     LNIISHEDSLLTLGALSETEQYSRIDDIIAAEEAESLRKKQEEEAAKAKEQEEQSNMQ
                     FSQFQNQPGKDGMPGNSTGSSWYFYNQSAISFGFSEFAKKWGNRKLEDNWRRSSKESI
                     ATFGSEEEAEAQARADSLEQAQKALNDSIRQLNSEARRKAYFNNIPKTAQQKEESNAK
                     ILEAYYNAGIIYKEALGQPKPAADDMEEMMKRYPGNKYELPAYYNLYRCYLALFDEPK
                     AEYYKKIILNDYGDSEYARIITNPNYFKDAQRKTAIMQVFYENTYKAFLNRQYTDVIE
                     RKSAADSLFPGGTLAPKFAYLQALSIGYTQPRPQFEASLKKIVAAYPRDSVSILAKAI
                     LDKMNNVTPIDTTISESATTQTAEGNKMLNSIYEFMPDKPHNVLIIFSAELPYATTLK
                     GKINQFNQRNYKEKNLSVTELRVDDKNYMMVRSFPDHTQSKEYIQGLDSDQTVFEGMD
                     KSAFTLFPITEKNFNKFITDKDLSKYLDFYRVNYP"
     gene            10749..11627
                     /locus_tag="PKU82_04190"
     CDS             10749..11627
                     /locus_tag="PKU82_04190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOZ82107.1"
                     /translation="MAKASGKEKVKLSTKLRNKFRLVIMNDETLEEKASLLLSPINVV
                     VVVGIAIITLITATTYIIAFTPLREYIPGYTDVKVKRAAIESLIRTDSLQKQVSAQDL
                     YIQNLQNLITGQSQKTVSLDTVSVPQLYDTIKQLLHSEEDSLLRSQIESPQDYNLTLN
                     AGTTKGSIANFYFFAPLKGAITTRFNPTEKHFGVDVVAGPDAVIKSAMDGTVVIAEWT
                     SQAGYVIAIQHSNNMFSVYKHNSALLKKVGNVVKAGEVIAIVGNSGELSNGPHLHFEL
                     WYNGAPVDPTDYIAFE"
     gene            complement(11628..12680)
                     /gene="mutY"
                     /locus_tag="PKU82_04195"
     CDS             complement(11628..12680)
                     /gene="mutY"
                     /locus_tag="PKU82_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOZ82108.1"
                     /translation="MSISNKLILWYERNKRELPFRSTNDAYRIWLSEVILQQTRVEQG
                     LPYYKRFLESFPTVKHLAKASETEVLKLWQGLGYYSRGRNLLATAQHITKIFHGKFPS
                     NYFELKKLKGVGDYTAAAIASMAFNEPIFAVDGNVARVLSRLFAEKTTPNSTEGKKVF
                     QELADSIGNKKFPGLHNQAMMELGALVCTPRNPKCEICPLQIHCLAYQQKQQLNFPVK
                     SKKIKIKERHFCFVIILYKDKFYIRQRNGNDIWKNLYEFPLFETRTKFSSATISNFAK
                     EYGIKKYESGRNHIHKLTHQHINASYIVAYLSKPLSSKKFPELKAVDAEDFKNNFPHP
                     RLIEKILSENNYFNLL"
     gene            12784..13626
                     /gene="panC"
                     /locus_tag="PKU82_04200"
     CDS             12784..13626
                     /gene="panC"
                     /locus_tag="PKU82_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOZ82109.1"
                     /translation="MKLIKEIHSLSHIVSRKKHENISIGFVPTMGALHEGHISLTKQS
                     LLENEFTIASIFVNPLQFNNPDDLKKYPRTLDRDMQMLEAAGCNALFAPEASEVYIED
                     EQPLQFDLGNLANVMEGKFRPGHFAGVITIVHKLFSWVKPDVAYFGEKDFQQLAVIRK
                     MAKALHPDIIVTGCPTLREPNGLAMSSRNVRLSNTVRHDASIIYKAMQHAAQLKNILP
                     VYEVCSQVRSMIEEIAPFKVEYVEIADAVNLQSLQQWSDSQSPRIFVAVFADDVRLID
                     NAAL"
     gene            complement(13631..14506)
                     /locus_tag="PKU82_04205"
     CDS             complement(13631..14506)
                     /locus_tag="PKU82_04205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ82110.1"
                     /translation="MELLTPQNFKDYELLDSGNFEKLERFGQYITIRPEPQAVWNRQL
                     KEADWLKKAHVKFIAKNSSSGTWKKLKEIPDRWHIAYALPDEKQIRLRLALTSFKHVG
                     VFPEQAVNWDFIYNSVKKLDVVQPKVLNLFAYTGASTLAAKAAGADIIHLDSIKQVVT
                     WAKENMTLSGLDNIRWLVEDAMTFVKREVKRGKKYQGIILDPPAYGHGPNGENWKLED
                     EINEMLANVKLLLEENHFLVLNTYSLGFSSIILDNLLDEHFSKNIKRQTGEIFLQSTA
                     GARLPLGVFARHCTV"
     gene            14596..15729
                     /locus_tag="PKU82_04210"
     CDS             14596..15729
                     /locus_tag="PKU82_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ82111.1"
                     /translation="MIINTSELSGRSIQATDKTPLRILLVDDIPANLLTLEGILEDDS
                     RILIKAFSGNEALKLLMEEPIDLILLDIQMPDMDGVEVAQLLRANPKTKHIPIIFVSA
                     IAKSERPSLEEFEIGSFDFIPKPLDIEETQKIVSIFEFGCRYKQKYREQEEKLKRLND
                     HLDKFLYILSHDLNAPLRAIENLVQWLEEDLQSQISDESKENIGLLRNRVTKMKLLLD
                     GLLRYARSGNTKHQHDIISPEKMIRQITSELQGDRKVSLNVNSSVLDMNTDRTALQLV
                     LKELIQNAISFNPSDKNTIDISVVEKNDSYEFVIADNGPGIKPQQQKTIFELFQTLPS
                     SVVPSSGVGLSIVKKVVEEYGGTVWFSPTVKCGTEVHFNWKKS"
     gene            complement(15733..18126)
                     /locus_tag="PKU82_04215"
     CDS             complement(15733..18126)
                     /locus_tag="PKU82_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HOZ82112.1"
                     /translation="MSNTDTVNKVKCYHCGDDCDEQVITSDEKIFCCTGCKLVYEILR
                     ENNLCNYYNLNNNPGTKQSDIVQKRFAFLDEPSIENQLISFRDKNETIITFYIPAMHC
                     SSCIWLLENFQKADEGVLSSRVDFVRKELRLVYDHHKTSLRKIAEKLSQTGYEPALQL
                     DAIEKKKIKKANRSRILRIGVAGFCSGNIMMLSFPEYFSSGNNIDASLQTLFSYLILL
                     LALPVFFYSANEFFIKSWQALKQKTTSIDLPIAIGISVIFLRSAFEIITQTGSGFFDS
                     GSGLVFFMLIGRWFQDFTFDSLAFDRDFKSYFPIAVTSIKNGNEKEVQINDLKVNDRI
                     LIRNNEIIPADCILLKGNASVDYHFVTGESLPVSHTAGEYIYAGGKQAGAMIELQVMK
                     EVSQSYLVQLWNRSWDKQDISKFEKLVNAISRWFIVVTFSISTIAAIYWWNTDIHKAI
                     NAFTAVLVIACPCALALSAPFTYGNILRILGRNKIYMRNYRVLEKIADADTIVFDKTG
                     TLTKNNVSEISYHGQSLDDEDKKILSSLFRQSSHPLSKLLQQHLQADKYYPCNNFEEV
                     TGKGICAVLNNKNIKAGTSDWAGVNNEVEKNATAVFININDIKKGKFVFSNIYRDGLN
                     TISQKLNNKHNLIVLTGDNESERKNLTTLLGNAELRFNQKPEDKRNYILELINSKKNV
                     LMIGDGLNDAGALLAANTGISVTDNTNNFTPASDTIMDAAALHQLPELIKLSKSAVTI
                     IIISFIISLLYNFVGLAFAVSGTLSPLFAAILMPISTISLVLFTVGASTIKARQSGF"
     gene            18262..19638
                     /locus_tag="PKU82_04220"
     CDS             18262..19638
                     /locus_tag="PKU82_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ82113.1"
                     /translation="MQIKQFYNQGHSQFSYIISSGKEAVIIDPVRDVEHYLQYLADKQ
                     LILKYIFETHIHADFVSGHLELADKTGATIVYGPQVRVAYPSYSARHNESFNLEGCSI
                     KALHTPGHTMESVCYLLNNNVGQPIAIFTGDTLFVGDVGRPDLMSGNKNAVELSEYLY
                     HSINDIIKTLPDELVVYPGHGAGSFCGKNPGKEKFSTIGEQKKRNYALKDISLEKFTN
                     EITTGLSVPPEYFFKVMSINIMGCRPMKDVFRNNLNFLSPVNFKAETQRALIVDTRNE
                     LEFSKAFIKGSVNIGLDGSFATWAGILLDPDHPVLLVCDSGKEKEAIGALAQIGIENV
                     RGILHGGIKSWKDAGQPVDNVENIDAEDFYFLFENEGYILVDLRSHQNFNAQHIKRSL
                     SIPLDELPHRLNEFSPDTKYLLVCKGGYHSMIAASILKRNGINQVVNISGGFDAVSAF
                     QPQYVESI"
     gene            19921..20742
                     /locus_tag="PKU82_04225"
     CDS             19921..20742
                     /locus_tag="PKU82_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ82114.1"
                     /translation="MKKIVVTTDFSECSENALIYSLSLCKEVNASLYLLHVFHIPVPV
                     TDMPYISVSSEEEEKNIELRLKELITRIRHKTGYEGMIHTITDFNFTVDGILNVVNDT
                     NADLLVMGQRGKTTLADRLFGSTATAIINLATCPLLLIPEGCSFSKIKKIAIACDKKT
                     SDNKNKVFHVVEMNAALKSEMLLFNVVQDEAEKDRLKGEDFTEMAGKEVKLFFPVHHD
                     IHEGILQFVNDNDADWLCMFPHRHNIFEQWFTRLNSGNISQQVKIPLLLINELVQ"
     gene            20821..21234
                     /locus_tag="PKU82_04230"
     CDS             20821..21234
                     /locus_tag="PKU82_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82115.1"
                     /translation="MKHRLTIQAEDTILDFQKKFNRIYPFLKIEFFEKAHIAKQGSHK
                     KEMIASNMLFRGLIGRGKSHIINFNDDFSISDFEMLLNDQCRLYAQVFRKSGKIWLET
                     SATDYWTLEQQNEEGKSLAKHFKTESENINDIDPY"
     gene            complement(21428..>22278)
                     /locus_tag="PKU82_04235"
     CDS             complement(21428..>22278)
                     /locus_tag="PKU82_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82116.1"
                     /translation="SLAPFQSGNIVVTVNVHTGLPIGSLINSSVRIEPVAGDANPGCN
                     YSSWEVFTTGSFDPNDILVSRDTLFTTEIPNPPYLDYIIRFQNTGNDTAFNVKILNPL
                     DTNKLQLNTLEYVASSHPVNMNFIYHERNMEFKFDNILLPDSNVNEPLSHGFIHYRIK
                     PKTSLVLNDSIQNTAAIYFDFNDPVITNTATTHVVLPTGIASYQSAENIQLFPNPAQS
                     ELNIQLTTAVEKIQVIDVLGKILFEKKTNNQQNLKINISQFQNGVYFVKAYSTQGVVV
                     KKFVKR"
BASE COUNT         6988 a         3936 c         4150 g         7204 t
ORIGIN      
        1 acactgcaat gccaaatgcc gatgcaataa tcaacacatc aacattagca tacagaaacg
       61 gtgaaataaa ttatcttgaa tatgcagcag cacttgaaaa tgcaacagca atacgaacag
      121 gctttctgca aagtattcac gatgtaaatc aaacagtcat caacataaat tctttaacca
      181 ataaataaaa atcagaacaa tgcaaacgat aaagtattat ttatatatca tattaatttc
      241 gctgctgaca ggttcatgca gcacaaaaaa cacaaccggt gaagccgaaa accctgttga
      301 tgcacatgca catgaaagtg caactacaac cacactcact tcagagcaaa taaaatctat
      361 tggtgtcaca acaggagggt ttgaaaagaa acaattgaca gcttcactga aggcaaacgg
      421 aattttaaaa gttccgaatc aaaatcgtgc aaatgcaact tcattacttg gcggtacagt
      481 gaaagagata ttagttcaga caggagcatc ggtacagaaa gggcaaacca ttgctattat
      541 cagtaacaat cagttcatca gtatgcagga agaatatctc agtataagtg cacgtgcagg
      601 tcttgcagaa gcagattttg aaagacaaaa aacgttgaaa gaaggtaatg cagcatcgtt
      661 gaaagccttt cagcaggcag aggcagaact gaagtcatta cgtgcaaaaa aatcaggctt
      721 acaaaagcaa ttgtctttaa tcggtattga tgcccagcag ttgactcctg aaaatattca
      781 atcgtcagta aatattaaaa gccccattaa tggatctgta agcaatgtac tggtaaatat
      841 cggaagttat cttgaagcaa gtaatcctgt tgcagagatt gttgacaaca gtcaattgca
      901 tcttgacttg tatgtgtatg aaaaagacct caacaaggtt aaagtaggac agacaataca
      961 tttcacactc accaataatc cgggtaaaga atacgatgct gatatttatg ctatcagtaa
     1021 tacgtttgag ccaaacacaa aagcagtctc tgtacatgca actgttaaag gtgacaaaca
     1081 tggactgatt gatggcatga gcattactgc tttgataagc ttagaaaacg caacttattt
     1141 ggcagtaccg acagaagcaa tcgtttcaaa tgaaggccag gattttattt ttattgtctc
     1201 tgataaagaa gttccaccac aagaggccgc tgcacatgac catagtgcac atgatcatgc
     1261 agcacatgac catagtgctg aacccgttac agagtccggt acagtgtttg aaagaattcc
     1321 tatcagaaaa ggaacaacag atgtgggtta ttcggagatt actttattaa aagatattcc
     1381 tgccgatagt aaggtggtta ctaaaggtgc attttttatt ctggctaaga tgaccaatca
     1441 gggtgaggca catgagcatt gattattaaa aacttaataa agtaacatct gagtaactta
     1501 tttggatttg attgattaaa tttgacaact gaaagaatat attatgagaa atataaaaag
     1561 caacatcaaa aatattttta ttgcaactat tatcggattt atgctgcacg gatgtagtag
     1621 taaaccaagc gaaacagtaa acagcactaa ggtgagtaaa gcagttaatg atgagcctgt
     1681 aaacgaacct gcagagccta ttgagctgga taatggccaa aagtggaaag taaatgagga
     1741 gatgaaacca tttcttacaa atggcaaaaa cttgttgaat gaatacattc aaaacgaaga
     1801 taacgactat acaggtttag cagcagcact tagtgagcag gataataagt tgattgaaag
     1861 ctgtacaatg gatggtaaaa gccacgaaga attacataaa tggctgcatc cacatttaga
     1921 tatggttgcc gatcttgaga gtacaaaaga tacagcacaa gctaaaaagt tggtggaaca
     1981 actgaaggcc tcttatgttg attttgaaaa gtactttcaa taagtactga aatatttttt
     2041 gtttacttag taaactgcag gcagaagtag gttctactga gtgtgataat aatgagaggc
     2101 aaaacactgc tatatatgac attcgttagt cagattaaat aaaccactta actcaaaaat
     2161 gataggacga tagcataaat gacagaattt tttaatcaac tacttcatga gatcgaacga
     2221 ccacttacca atccggtact gattttttct ttgatattat ttatcattct gctttctccc
     2281 attttgctga ggcgtttgaa tattcccgga atcattgggc tcatcattgc aggagtaatt
     2341 atcgggccac atggtttata tattctcgaa aaaaactcag caatcaatct gttttcaacc
     2401 attggtttgc tttacatcat gtttattgcc ggacttgaac tcgatctgaa tgaatttaaa
     2461 tctcatcgca ataaaagttt ggtctttggt ttttttactt tcatcattcc tttaggtatt
     2521 ggctttccgg tttgtcatta cttgttgtat tatgacttta atgcaagtct tctgacagca
     2581 agcatgtttg ctacacatac gcttgttgcc tatcccattg taagcaagtt tggtgtttca
     2641 aaaaatcagg ccgttgccat tacggtagga ggaactattt taacagacac tgccgtactg
     2701 attatccttg cagtaatcat gggcaacagc cagggaaacc tgaatcagga attctggatt
     2761 cgtttagggg catcactgat tattttttct gcaataatgt ttattgttat tccgagaatt
     2821 gcaaaatggt ttttcagaaa actcgaaagt gaaaaacatt cgcattatat ttttgtactc
     2881 tctgtggtgt tttttgcagc ctttcttgca gaggttgccg gagtagaacc aatcatagga
     2941 gcctttgttg caggtttggc attgaacaaa ctgattccac attcatcagc attgatgaac
     3001 agaattgagt ttattggaaa ctctttgttt attccatttt tcctgatcag tgtagggatg
     3061 ttggttgatg ttagtgttat catgaatgga cctactgcac tcatcatagc aggtacatta
     3121 acaattgttg caataacagg aaaatggctt gcatcttttc tgacacaagt tatttttaaa
     3181 tatacatcat cgcaacggca acttattttc ggtttaagca gtgctcatgc tgctgcaaca
     3241 cttgccgtta ttttagtagg ttataaagca ggcattcttg atgagaatat cctcaacggc
     3301 accattattc ttattttaat tacatgtatc attgcttctt ttgcaacaga gcgtgccgct
     3361 aaaaaaataa ttgtagaaac agaagatgac agcagcgatc ttttaaaaag tagcagtgct
     3421 tccaatgaac atattttact tcctgtagcc aatgttggta aaatggaaaa gctgcttgac
     3481 tttgcaattt taataaagga taaaaaatct gttaacccag tgtctgttct ttctgttgta
     3541 tctgacaata gcgaggcaga gaaaaatatt ttaaaggcaa aaaataaatt agaagatttt
     3601 gttaaacaag cctcaggaac agaaactaaa gttaacgtaa ttactacaat tgatcataat
     3661 gcagcaagcg gaattacccg ttgctcaaga gaaattatgg ccgacatcat tgttttggga
     3721 tggccacagc gtacaggatt tcttgataaa cttattggtg aaaaaattga aagtatatta
     3781 aacaatacag ataagacaac tttaatttgc tttcttgaaa aaccacttga acttcataag
     3841 cgaatagtgg ttgctgtacc acctcttgcc gaacatgaaa atggatttgg tttatggctt
     3901 actaaattgg caaagctggc acaggaactc agtattccaa ttcatacata ttgtaatgaa
     3961 gcaactgaaa aatcaataag aaaatttttc agtaaagaaa aattagcagc atcactgagc
     4021 gttagtttgt ttagcgactg ggatgatttt ctggtactgg caagaaatat tcatgctgat
     4081 gattttgttg ttttggtttc tgccagacgt ggtgcaactt cttacatgag tattttagat
     4141 ggacttccgc tgaaaataga aaaacatttt agcgacaaca gccgagttat tatttatccg
     4201 caacaatatg acggcaatat taatctggaa aactatagcg aggtttcaac agaaacttta
     4261 aacagaggta ttgaggtggt tcagaaaatc ggtaaagaaa ttggctcaat gctaaaaaag
     4321 aaaaatcaag agtagattta ttcttgatta atcattttga tttataaaca ggaacagtag
     4381 aacatggttc gccatacata atactcttaa caacaggttt tagtttttgt gtcaacagca
     4441 tataaacagc aggcgaaaag ttttcatcac cacacccttt aattactatt cgtttgttat
     4501 tatagttttg aatgttgatt ttttcaattg tatcaatggt tagcttttct ttgagttgag
     4561 tagcgttacc aaaaaaaatg ttggtggtat gtggctgcaa tgcttcacta acgagcataa
     4621 atgcccaagt cggaataatg gcatcaacag aacaataaac agcaactgca acgccatcat
     4681 actgctgcca gttatgttgt ctgatatgct ctctgaaatc tttttcttta agaatcattt
     4741 cctcaaacaa ccatccttta atgtcaagtt cggttattgt tgtggcaggt ataaaatctt
     4801 ttggatctat ctgtagaata ccactgtcgg caacgcgatt tttaatttcc tgttccataa
     4861 ttaccacatt ccaagttcca tgcgagcctc ttcactcatc atatctttat cccatggtgg
     4921 atcaaaagtt aattcaactt ttgcatcatt cacatcagga atacttttta ttttttgttc
     4981 aacttccaat ggcagcgatt ctgcaaccgg acaagcaggt gaagtcagag tcatctttac
     5041 atgaacatca tttccttcaa taatctttac ttcgtaaatc aatcctaact cataaatgtc
     5101 aaccggaatt tccggatcgt aacaagtctt tatggtttct ataaccctgg catataattc
     5161 ttttggctct tccataagtg caaatttaat ggttttattg ttgtcttgat tttattgctg
     5221 ttttattctg attttacaat caatagaaga ttcaaaataa attcatgcaa agcatcaata
     5281 ttaatcccat ctttgaaaac tataaagtca agtaaaagtt ttttaaaaat aaatattatg
     5341 aaacaaattt taatcgttct ccttacaatt ggtctatcat tttcatcatc agcgcagcaa
     5401 aaaactgttt tggaggccaa tgaaaaagtt gctataatca gtttcgaaaa aatagaacat
     5461 aatttcggta agattaaaga aggtgataag gccgaacatg tttttctttt taaaaataca
     5521 ggcaacacac cgttaattgt tactgacgtg agtgttacct gcggatgtac ggcagcagaa
     5581 aaacctgatg cgccaattat gcccggtaaa gacggaaaaa taaagatagt atttaactct
     5641 gccggtaaag ttggttcaca aaaccgcaca ataacggtaa gtaccaatgc aaaaaataaa
     5701 attacttatt tgaaaatgat aggtgaagtg agtgaaaaat aatttctgag aaaatggtta
     5761 aactctttct ccaaattcca tcagaaaggc tttcataaac tcatgtagtt cgccatccat
     5821 tacggcttga acgtttgatg tttcacaatc tgtgcgtaag tctttaacca tcttatatgg
     5881 atgaaaaaca tagcttctta tttgtgagcc ccattcaatt tttttcttac ctgcttctgt
     5941 cgctgcctta gcctcattgc gtttgcgaag ttcaagttca taaagttgcg aacgcaacat
     6001 ctgcatggca cgctcgcggt tgccactttg cgaacgttct acctgacata caacaacaat
     6061 acctgtgggc aagtgtttga gttgaacctt tgtttctact ttatttacat tttgtccacc
     6121 tgcaccacct gaacgggaag tctgcatttc tatatctgca tctttaattt caatctctat
     6181 agtatcgtca atcaaaggat aggcaaacac actggcaaat gatgtatgac gttttgcatt
     6241 ggaatcaaat ggagaaatgc gtactaaacg atgtacacca ttttcgctct ttaaatatcc
     6301 gtatgcaaaa tcaccttcta tttctaaagt aacagatttt attcctgcaa catcgccatc
     6361 ttgtctgtca atttcgttta tgcgatagtt catccgttcg gcccagcgga tatacatacg
     6421 catgagcatt gatgcccagt cgcaactttc tgttcctccg gcaccggcat ttattgtgag
     6481 cactgcactc aatgcatctt cttcaccact gagcatgttt ttaaattcaa gctcttctaa
     6541 tgatttttga acggaggtat attgctcttc tacttcttgt tctgaagcat cgcctgtttt
     6601 tgaaaaatcg aataatacac ccaaatcatc aacagcttgc tgtacggcag caaacgtgtc
     6661 tgtccagttt tttttaagct tgatggattt taaaatctgt tctgcccttg tagggttgtt
     6721 ccagaaatca ggtgcgtgtg taagttgttc ttcttctttt attttaagca gtttgacatc
     6781 aatgtcaaag gtgccccctc aaagcgtcca cgcgctcctt acattctgtt aattgttctt
     6841 gtgtcatacg ggctgcgaat ttacgaatta atgttttccg gcattttcag actatgttgt
     6901 cttcatcagc gtataggctt ttgctacatc aaaaatcata aacaaaaaat aggctaagca
     6961 aaaactaatt acaaaaggaa ctgccattgt ttgattcaga aaaatgtagc ctacaatgat
     7021 agcaagcatc agaaataatt taaacgctgt tgatgccata aaaaaacgga tggactcatt
     7081 acctttactc ttggtaagtc gcaaataatg aatgacagaa aaaagtacga agaaaaatat
     7141 tccgcatttc caccatgaag gatagaaacg tgcagccaga aaatctttca tcaaataata
     7201 aagcaaaata tacagcaggg ttatgccggc aagttgtaaa atataggtga tgaaaccttt
     7261 gttcattttt tcttatgtaa aaaatctttc aatgttaagt aaagtgcagc tgcaacagca
     7321 atcaaggcaa aagtaacagt gaatagtgga attttgtttt gacaatattt gtctaagcgg
     7381 taacctccat aggtaccaat aaacatgata gcgcccattt gaaatcccat ggacgcatac
     7441 cgggcataat cgttaagctg atttttcctg gagtttggct tctgatcttt cgacattttt
     7501 tatgtcttta accataggcc ccatattaca gttgcctgta aaacttgccc caacttcaac
     7561 aactaatttg ccggtgttaa tgtcgccatt gatttttgca gttgacttta gaaacaacat
     7621 ttcagtaacg gtagatgttc cttttactgt tcctgaaaca tctgcattct ggcaaacaat
     7681 gtctccatcc actactccgg ttgtgccaac aaccacttta gcttttgaaa atactgagcc
     7741 tacaatgtgt ccgtcaattc gcaagtcgcc attcgacttg atttcacctt ctattgaagt
     7801 tccggcagca attaagttaa ttgagcctga tgcagtttca ccgggtttaa cggtggtggt
     7861 tttgttcttc aaattttcaa acatggtaaa aacttttaat tatgtattgc ggcaaagata
     7921 aaaataaaag ttattaacaa aaccaaaaat tatggataat taacacggta gaaatcaagg
     7981 tatttgctca aatccttatc ggtaatgaac ttgttgaaat ttttttctgt aatggggaaa
     8041 agggtaaaag cacttttatc catgccttca aaaaccgttt gatcagagtc caacccctga
     8101 atatattcct tgctttgcgt gtggtcggga aatgaacgca ccatcatata gtttttgtca
     8161 tcaactctta actctgtcac actcagattt ttctctttat agttgcgctg gttaaactga
     8221 ttgatttttc cttttaatgt tgtggcataa ggcagttcag cagagaaaat aatcagcaca
     8281 ttatgtggtt tgtccggcat aaactcatag atagaattca gcattttatt gccctcggca
     8341 gtttgtgttg ttgccgattc gctgattgtt gtatctattg gcgttacgtt attcatttta
     8401 tcaagaatag cttttgccaa tatgctgaca gaatctcttg ggtatgcagc aacaattttt
     8461 ttcagtgaag cttcgaactg tgggcggggc tgtgtatatc cgatagaaag tgcctgaagg
     8521 taggcaaact ttggcgctaa tgtgccaccg ggaaatagtg agtctgctgc cgatttacgc
     8581 tctatgacat ctgtatattg acgattgaga aaagccttgt atgtattttc ataaaacacc
     8641 tgcatgatgg ctgtcttgcg ctgtgcatct ttaaaataat ttgggttggt tatgatgcgt
     8701 gcatattctg aatcgccata gtcattgaga ataatttttt tataatactc ggctttaggt
     8761 tcatcaaata aagctaagta acaacgatac agattgtaat aagcaggtag ttcatattta
     8821 ttacccggat atcgtttcat catttcttcc atatcgtcag cagcaggctt tggctgacct
     8881 aatgcttctt tataaatgat tcctgcattg taatatgctt ctaatatttt agcattactt
     8941 tcttcttttt gctgagcagt ttttggaatg ttgttaaagt atgcttttct gcgtgcttcg
     9001 ctattcaatt gtctgatgct gtcgtttaat gctttttgtg cctgctctaa actgtcggca
     9061 cgtgcttgcg cttcggcttc ttcttcactg ccaaaagttg caatggattc tttgctgctc
     9121 ctacgccagt tatcttcaag ttttctgttg ccccatttct tggcaaactc cgaaaagcca
     9181 aaactaatgg cactctgatt ataaaaatac catgagctac ctgttgaatt tccaggcata
     9241 ccatcttttc ctggttgatt ttgaaactga ctgaactgca tgttagactg ctcttcctgc
     9301 tctttagctt tggctgcctc ctcttcctgt ttcttacgca gcgactctgc ttcttctgca
     9361 gcaataatgt cgtcaatgcg cgaatattgt tctgtttctg aaagtgctcc caatgtgagc
     9421 agactgtcct cgtgtgaaat gatgttcagg ttttttatca atcgcgacag attggttttt
     9481 ttattctgaa tcaatgcata atcaggatag tcagaagtga tattggtggc agcactgtca
     9541 taatataatt gcgcagcacg gaagtcaagt ttgcggaaac ttatttcacc catctccaga
     9601 tatgaaatac ctttttgatt ggcgttgttg gtgcttgctg ccacagactt ctttaaaaat
     9661 tgaaatgcct ctgttgtatc tttttcacgc atagaaattc cactcagtgc ataataaatc
     9721 tgatcaagat aatccttgtt tttttcatct ttaagcatct tgttcaactc atcttttact
     9781 tgttgtgcat cggcactgtt tacatcaaat gaacgcgctc tgttgatttt tgcattaaat
     9841 gccatctcat actcgggatt catcttgatt actttgcggt agattgcaaa agctttgtcg
     9901 ttggaatcaa ccttctgata tatctgtgca aggatatagc tatatcggat tttgtcagct
     9961 ctctttgtcg ccagatcagc agctttggtt aattgctcaa tagctttagg ataattttct
    10021 ttcttcagat aaaaatctgc agccacagca gcaaaaagag gtttgtactt tgatggcaat
    10081 tgcttttcat tttttaaata gtcgagcatg tattctgcat cggccatttt gcctaactgc
    10141 atttgtgttt gcatctgcca gattaagcca tcaaatcttt ttgggttatc tttataggta
    10201 gatgcaacgt attgaaaagt ctctaatgca gtccagaaat catgctttag aaattgcgct
    10261 ttaccgatta tcatgtagtt gtccggtatc cagcggcaac gctctttacc attaataaac
    10321 atggagtgtc gttgaataac tattgagctt ttcttaatgg cctcatccat gtcagggtaa
    10381 atggtcttgg ctttatcgtc agttgcatat ttaaaaacat caagcacacg gtcgtaatga
    10441 tcaacatgca atgcagacaa agtttttgta ccttctttca ctctttcgcg ggcattaaaa
    10501 tagccgttgt agtgtgcagt aaggttatgg tatgccctgt ttatgacagt gttcttttgt
    10561 gttgaacatg ctgatagaaa cagcgtaagg atgataaaat attttacgtg attggatgat
    10621 ttcaaaacaa tatggttgtt gcgataaata ttaacttttt ccagcgaaaa ttattgctcg
    10681 tttaattatt acgatatttg cccgattaag aaggcaaaca taacaatact tttttgttta
    10741 aacaacgcat ggccaaggca tcaggcaaag aaaaagtcaa actaagcacc aaactccgta
    10801 ataagttccg gttagtcatt atgaatgacg aaactctgga agaaaaggct tcgttgctgc
    10861 tctcaccaat caatgtggtg gtggtggtgg gtattgccat catcaccctc atcacagcca
    10921 caacctacat tattgccttt acaccactgc gtgaatatat tcccggatat acagacgtta
    10981 aagtaaaacg ggcagccatt gaaagcctta tccgtaccga ttctttacaa aaacaggtgt
    11041 ctgcacagga tttgtacata caaaatctgc aaaatcttat taccggtcag tcacagaaaa
    11101 ctgtttcgct cgatacagtt tctgtgccac agctttatga tacaataaaa caattgcttc
    11161 attccgaaga ggattctttg ctgagaagtc agatagagtc gccacaagat tacaacctaa
    11221 cattaaacgc agggaccact aaaggttcta ttgctaactt ttactttttt gcgccattaa
    11281 aaggagcaat aacaacacgg tttaatccta cagaaaagca ctttggtgtt gatgtagttg
    11341 ccggccctga tgctgttatt aaaagtgcca tggacggaac ggtggtcatt gcagaatgga
    11401 cttcgcaggc aggttatgtc atagccattc aacacagtaa caatatgttt tctgtttata
    11461 aacataattc tgcattgctt aaaaaagtag gtaatgtggt gaaagccgga gaagttattg
    11521 ctattgtagg taactcgggc gaattaagta atggtccgca tctgcatttt gaactttggt
    11581 acaatggtgc gcctgttgac cctactgatt atatagcctt tgaataatta cagcagatta
    11641 aagtagttgt tttcagaaag tattttttcg attaaccttg gatgtgggaa attgtttttg
    11701 aaatcttcgg catcaactgc ttttaactca ggaaattttt ttgatgatag tggcttgctt
    11761 aaatatgcca caatgtatga tgcattaatg tgttgatgtg tcagcttatg aatatgattt
    11821 cttcctgatt catatttttt aataccatat tcttttgcaa aattgctaat agtggctgat
    11881 gaaaattttg ttctggtttc gaacaaagga aactcataga ggtttttcca gatgtcgtta
    11941 ccattacgtt gtctgatata aaatttgtct ttgtaaagta ttatgacaaa acaaaagtga
    12001 cgctctttga ttttaatctt tttacttttt accggaaaat taagttgttg cttttgttga
    12061 tatgccaaac aatgaatttg taaaggacat atttcacact taggatttcg tggtgtacaa
    12121 accaatgcac caagctccat cattgcctga ttgtgcaggc cgggaaattt tttattgcct
    12181 atactatcgg ctaattcctg aaacactttt tttccttcag tgctatttgg cgtagttttt
    12241 tctgcaaaca atcttgataa tacacgagcc acattaccat caacagcaaa gataggttca
    12301 ttaaatgcca ttgaagcaat agctgctgca gtataatcgc ctacaccttt tagtttttta
    12361 agctcaaaat aattggaagg aaatttaccg tgaaatattt tggtaatatg ttgtgctgtg
    12421 gctaaaagat ttcgccctct tgaataatat cctaatccct gccacaattt gagcacctcg
    12481 gtttctgatg ctttggcaag atgcttcact gtagggaacg attctaaaaa ccgtttgtaa
    12541 taaggcaatc cctgttctac tcttgtctgc tgaaggataa cttcgctcaa ccatattcta
    12601 taggcatcgt ttgtactgcg aaaaggcaac tcgcgcttgt tgcgttcata ccataaaata
    12661 agtttgtttg aaatactcat ttgctttggc aaagaaagtc attataaaaa ataattacag
    12721 tttaaattta atagacaatt cagtaacaac taaatttact tatcattgcc atcgcttttt
    12781 gttatgaaat taattaaaga aatacactct ctttctcata ttgttagcag aaagaagcac
    12841 gagaatatca gcattggttt tgtgccaaca atgggcgcat tacacgaagg acatatttca
    12901 ttaactaaac agtcgctgct tgaaaatgaa tttactattg ccagcatttt tgtaaatcca
    12961 ttacagttta ataatcctga tgatttaaaa aagtatcctc gtaccttaga tagagatatg
    13021 cagatgcttg aagcggcagg ttgtaatgcc ttgtttgcac cggaagcatc agaagtttat
    13081 attgaagatg aacaaccatt gcagtttgat ttaggcaatc ttgcaaacgt tatggaaggc
    13141 aaatttcgtc cgggacattt tgccggagtt atcactattg ttcacaaact tttttcatgg
    13201 gttaaacctg atgttgctta tttcggagag aaagattttc agcagcttgc cgttatcaga
    13261 aaaatggcta aagcattaca tcctgatatt attgtcacag gctgccctac acttcgtgag
    13321 cctaatggtc ttgccatgag ttcgcgcaat gtaagattaa gtaataccgt tcgtcatgat
    13381 gcatccataa tttataaggc aatgcaacat gctgcgcaac tgaaaaacat actgcccgtt
    13441 tacgaagttt gttcgcaggt gagaagtatg attgaagaaa tagctccttt taaagttgag
    13501 tatgttgaaa ttgctgatgc agtaaaccta cagtctttac aacaatggtc agactcacag
    13561 tcaccacgta tttttgttgc agtttttgca gatgatgtaa ggctgattga taatgcagca
    13621 ttgtaaataa ctacaccgta caatgccgtg caaagacacc taatggaagt ctagctccgg
    13681 cagtgctttg tagaaaaatt tctcctgtct gtcgtttaat atttttacta aaatgttcgt
    13741 caagcagatt atctaaaatt atagatgaaa aaccaagtga ataggtgttg agtacaagaa
    13801 agtgattttc ttcaagcagc aacttaacgt tggcaagcat ttcgtttatc tcatcttcta
    13861 atttccagtt ttcgccattt ggtccatggc cataagcagg tggatctaaa ataattccct
    13921 gatatttttt cccgcgtttt acttctcgtt tcacaaaggt catggcatct tcaaccaacc
    13981 agcgaatgtt gtccaagcca ctcaatgtca tattttcttt tgcccatgtt acaacctgtt
    14041 ttatagagtc taaatgaatg atgtcggcac cggcagcttt ggcagcaagt gttgatgcac
    14101 cggtgtaggc aaagaggttg aggacttttg gttgtactac atctaatttt ttaactgaat
    14161 tataaataaa atcccagttt acggcctgtt ccggaaaaac acctacatgc ttaaatgatg
    14221 ttaatgccag cctaagtcga atttgttttt catcaggcaa tgcataggca atatgccaac
    14281 ggtcaggaat ctctttaagt tttttccatg tacctgacga gctgttttta gctataaact
    14341 ttacatgtgc ttttttaagc caatcggcct cttttagttg cctgttccac acagcttgtg
    14401 gttcgggcct gatggtaatg tactgaccaa aacgctctaa cttttcaaag tttccgctat
    14461 caagcagttc ataatcttta aaattttgcg gtgtaagtaa ttccatgcag aagttcattt
    14521 aaatacctaa aagtgttgta atagcaacct ttttaatcgg gctgcgtaaa aacaaggcaa
    14581 tattaagtct aaaaaatgat aatcaacaca tcagagctgt cgggtcgtag catacaagcc
    14641 acagacaaaa caccattaag aattctatta gttgatgata ttccggctaa tttattgaca
    14701 cttgaaggta ttcttgaaga tgatagccga attcttataa aagctttttc gggtaatgaa
    14761 gctttaaaat tactgatgga agaaccaatt gatttgatat tattagacat tcaaatgcct
    14821 gacatggatg gcgttgaggt agcacaatta cttcgtgcca atcctaaaac aaagcatata
    14881 cccatcattt ttgtttctgc cattgccaaa agcgaacgcc cttcactcga ggagtttgaa
    14941 atcggatcgt ttgattttat tccgaagcca ttagatattg aagagacaca gaaaatagtt
    15001 tcaatctttg aatttggatg tcgctataaa caaaagtaca gagaacagga agaaaaactg
    15061 aaaagactga acgatcatct tgataagttt ctttacatcc tttcgcatga tttgaatgca
    15121 ccactgcgtg ccattgagaa tcttgtgcag tggcttgaag aagatttaca atcgcagatt
    15181 tctgatgaga gtaaggaaaa catagggctg cttagaaaca gagtaactaa gatgaaatta
    15241 cttcttgatg gcttattgcg ttatgcgcgt tccggtaata caaaacatca gcacgatatt
    15301 atttcacctg aaaaaatgat cagacaaatt acatctgagc tgcagggtga tagaaaggtg
    15361 tcactgaatg tcaactcttc ggttttagat atgaatacag acaggacagc attgcaactt
    15421 gtgttgaaag agttaattca aaatgcaata tcttttaatc cttccgataa aaatactatt
    15481 gatatttctg ttgtagaaaa aaatgattct tatgaatttg ttatagcaga taacggaccc
    15541 ggaataaagc cacaacaaca aaaaacaatt ttcgaacttt ttcaaacgct tccatcatcg
    15601 gtagtgcctt catcaggtgt aggccttagc attgtgaaaa aggtggttga agaatatggt
    15661 ggaacggtgt ggttttcacc aacagtaaaa tgtggtactg aagttcactt taactggaaa
    15721 aaatcctgag ctttagaacc ctgattgccg tgcttttatt gtacttgcac caactgtaaa
    15781 aagtacaaga ctgattgtgc taataggcat taatattgct gcaaacaatg gcgaaagtgt
    15841 tccacttacg gcaaaggcta atccaacaaa attatagagt aaagaaataa taaagctgat
    15901 gataattatc gttacagcag atttggatag tttaattaat tcaggcagtt gatgtaatgc
    15961 tgcagcatcc ataatagtat cacttgcagg agtaaaatta ttagtgttgt cggtaactga
    16021 aattcccgta ttggctgcta gcagcgcacc tgcatcattc aggccatcac caatcattaa
    16081 cacatttttc ttactattga ttaattcaag aatgtaattt cttttgtctt caggtttttg
    16141 attaaatcgt aattcggcat tgcctaaaag tgtagttaga ttttttcttt cactttcatt
    16201 atcacctgta agaacaatca aattatgttt gttattcagt ttttgtgata tggtgttcaa
    16261 cccatcacga taaatattac tgaaaacaaa tttgcctttt ttaatgtcat taatgttgat
    16321 aaaaactgct gttgcattct tttcaacttc attgtttact cctgcccagt ctgatgttcc
    16381 ggctttaatg tttttattgt ttaatactgc gcaaatgcct ttaccggtaa cttcttcaaa
    16441 attattgcat ggataatatt tatctgcttg caaatgttgt tgcagtaatt tacttaacgg
    16501 atgtgatgat tgcctgaata gcgatgatag tattttttta tcctcatcat caagcgattg
    16561 accatgataa cttatttcag aaacattatt cttagttaat gttcctgtct tatcaaatac
    16621 aatagtgtcg gcatcagcaa ttttttcgag tactcgatag ttacgcatgt agattttatt
    16681 tctgccaaga atgcggagga tgtttccata agtaaaaggt gcagataaag ccagtgcaca
    16741 gggacatgca attacaagca ctgctgtaaa tgcattaata gctttatgaa tatctgtatt
    16801 ccaccaatag atagcagcga ttgtagaaat agaaaatgta actactataa accaacgact
    16861 gatggcattc actaattttt caaacttgct tatatcttgt ttatcccagc tacgattcca
    16921 caattgtact aaatagctct gcgaaacttc tttcatcacc tgaagttcaa tcatggcacc
    16981 ggcttgtttg cctcctgcat aaatatattc accggctgta tgtgaaacag gtagtgactc
    17041 acctgttaca aaatgataat ctactgaggc attgccttta agtaaaatac aatctgcggg
    17101 aataatttca ttatttctta taagaattct gtcattaact ttcaagtcat taatctgcac
    17161 ttctttttca tttccgtttt ttatagaagt aactgcaatg ggaaagtatg atttaaaatc
    17221 tctgtcgaaa gctaaactat caaaggtaaa atcctgaaac caacgaccaa tgagcatgaa
    17281 gaaaacaaga ccactaccgc tgtcaaaaaa tcctgaacct gtttgtgtta ttatttcaaa
    17341 tgcacttcgt aaaaaaatta ctgatatacc tatggcaatc ggcaaatcta tggaagtggt
    17401 tttttgtttg agagcctgcc aagatttaat aaagaactca tttgcactgt aaaaaaatac
    17461 gggtagtgca agcaacaaaa tcagatagct gaatagtgtt tgtaatgaag catcaatatt
    17521 atttcctgat gaaaaatatt caggaaaact tagcatcata atatttccag aacaaaaacc
    17581 ggcaacacca attctgagaa tacggcttct atttgctttt tttatttttt tcttttcaat
    17641 agcgtcaagt tgcagtgcag gctcatagcc tgtttgcgaa agtttttctg caatttttcg
    17701 aagcgaagtt ttatgatgat cataaaccaa acgcaattct tttcgaacaa aatcaactct
    17761 tgaggataga acaccttcat cggctttctg aaaattttca agtagccaga tacaactgct
    17821 acaatgcatt gcaggaatgt aaaaggtgat gatagtttca tttttatctc tgaaacttat
    17881 cagttgattt tctatggaag gttcatctaa gaaggcaaat cgtttttgaa caatgtcaga
    17941 ctgtttagtg ccaggattgt tgttcaggtt gtaataatta cacaaattat tttcacgcag
    18001 aatttcataa accaacttac aaccggtaca acaaaaaatt ttctcatcgc ttgtgataac
    18061 ctgttcatca cagtcatcac cacaatgata gcatttcacc ttatttacag tatctgtatt
    18121 gctcatagcc tccaacctaa tttgcctgac aatattacaa ttgatgaaga atgtagctgt
    18181 tgatattaat tcttgatatg agtgataaaa gtcatttcag ttgttgtctc gaaataaaac
    18241 atttgccaca ttcaacttac tatgcaaatt aaacaatttt ataatcaggg tcattcgcaa
    18301 ttcagctata ttatttcaag cggaaaagaa gcggtaatta ttgatcctgt cagagatgtg
    18361 gagcactatt tacaatacct tgcagataag cagcttattt taaaatatat ttttgaaacg
    18421 cacatacatg ccgattttgt ttcaggtcac ctggagcttg ccgataaaac cggagcaacc
    18481 attgtttatg gacctcaggt gcgtgtggct tatccttcat atagtgcaag acataatgaa
    18541 agttttaatc ttgaaggatg tagtattaaa gctttacata caccgggaca tactatggaa
    18601 tcggtttgtt atttattgaa taataatgtt gggcagccta ttgcaatttt caccggtgat
    18661 actttatttg taggtgatgt aggtcgtcct gatttaatga gcggtaataa aaatgcagta
    18721 gaattgtcag agtatttata tcactctatt aacgacatca ttaaaacatt acctgatgaa
    18781 cttgttgttt atccgggaca tggtgccggt tcgttctgtg gaaaaaatcc gggtaaagaa
    18841 aagttttcta ccattggcga acaaaagaag agaaactatg ctttaaagga tatttcactc
    18901 gagaagttta ccaatgaaat aactacaggc cttagtgtgc cacctgaata tttttttaag
    18961 gttatgagca ttaatattat gggttgccgc cctatgaaag atgtgtttcg caacaatctg
    19021 aattttctgt caccggtaaa ttttaaagca gaaacacaac gtgcattgat agtggacaca
    19081 agaaatgaat tggagttttc gaaagcattt attaaaggct ctgtaaacat tggtcttgat
    19141 ggttcgtttg caacatgggc agggattttg ctcgatcccg accatccggt tttattggtc
    19201 tgcgacagtg gtaaggaaaa agaagccatt ggtgctttag cacagatagg aatagaaaat
    19261 gtgagaggta ttttacatgg aggaataaag tcatggaaag atgcagggca accggtggac
    19321 aatgttgaaa atattgatgc agaagatttt tattttttgt ttgaaaatga aggttacatt
    19381 ctggttgatt taagaagcca tcagaatttt aatgcacaac atataaaaag aagtctgagt
    19441 attccattag atgaattgcc acaccgtttg aatgagttta gcccggacac taaatatttg
    19501 ttagtgtgta agggtggtta ccatagtatg atagcagcat caattcttaa acgcaatggg
    19561 ataaatcagg tggtaaatat ttcaggaggg tttgatgcag taagtgcatt tcaaccacag
    19621 tatgtagaat cgatatagtg tgaacagaag gttactgaca atttaaacag tattaggtat
    19681 aaaacaggca tgaccttaaa tttatgacaa aagtcgcttt atgggttgct taaagtcatt
    19741 tttaggttat aataaaggaa atacttttac atcgttaatt aaaaggaaag caataaaatt
    19801 ccggtttgga aataccctcc ggtttaaaat aaaaagattt gatttagtgt tagggttgca
    19861 aaaccggtat gcttcggcta ccggtttttg cttaatagga ttatcgtaaa aagcataagt
    19921 atgaaaaaaa ttgttgttac tactgatttt tcggagtgtt ctgaaaatgc tttgatttat
    19981 tccttgtcac tgtgcaaaga ggtgaatgca tcattgtatt tacttcacgt ttttcatata
    20041 ccagttccag tgaccgacat gccatatatt tctgtttcgt ctgaggagga ggaaaagaat
    20101 attgaattac gtttgaaaga attgattacc cgcataagac ataaaacagg ttatgagggt
    20161 atgatacaca ccataaccga ttttaatttt acggttgatg gtattctgaa tgttgttaat
    20221 gatacaaatg cagacttgct ggtgatggga caacgaggta aaacaacatt ggcggacaga
    20281 ctgttcggaa gtactgcaac agcaattatt aatcttgcaa cttgcccttt attacttata
    20341 cctgaaggat gcagtttcag taaaattaaa aagattgcaa ttgcctgcga taaaaaaacg
    20401 agtgacaata aaaacaaagt atttcatgtt gttgaaatga atgctgcatt gaagtcggaa
    20461 atgctactgt ttaatgttgt tcaggatgaa gcggaaaagg atagacttaa aggagaagat
    20521 tttactgaaa tggctggaaa agaggtgaaa ttatttttcc ctgtacatca cgacatacat
    20581 gaaggaattt tgcaatttgt aaatgataat gatgctgatt ggttgtgtat gtttccacat
    20641 cgtcataaca ttttcgagca gtggtttacc agacttaatt caggtaacat ttcgcagcaa
    20701 gtaaaaattc ctttattatt aattaacgag ttggtacagt aaaaatattg ctaaaaagta
    20761 cttttatgaa tacgatgatt ttaaatctta taaagaagcg gttacgattc aatttaaatt
    20821 atgaagcata ggttgactat tcaggcagag gatactattt tggattttca aaaaaaattc
    20881 aacaggattt atccgtttct taaaattgaa ttttttgaaa aagcacatat agccaagcag
    20941 ggaagtcata aaaaggaaat gattgcttca aacatgttgt ttcgcggatt gataggacgc
    21001 ggaaaatcac atataataaa cttcaatgac gattttagta ttagcgattt tgaaatgtta
    21061 ctgaatgatc agtgtcggtt atatgcacaa gtatttcgta aatcaggaaa gatttggctt
    21121 gaaacttcag caacagatta ttggacattg gagcaacaaa acgaagaagg caaaagctta
    21181 gccaagcact ttaaaacaga gtcagaaaac ataaatgata ttgaccctta ctaattactt
    21241 cttctttcta cataggtaga aattttacta agtaatagct taataacaaa tgtgaataca
    21301 acaatctcat attatttgag aatatgactt gactgaatta ttggaaataa aattcaggag
    21361 attcttcaaa tccttaaatc tcttcagccg cggctgagtc gtggctgatc aacaatacaa
    21421 aaattttcta cctcttaaca aacttcttga ctaccacacc ttgtgtgctg tatgctttca
    21481 caaaataaac tccgttttga aattgactga tgtttatctt taaattttgt tggttgttgg
    21541 ttttcttttc aaacaaaatt tttccaagta cgtctattac ttgtattttt tcaactgctg
    21601 ttgtgagttg aatatttaat tctgattgag caggatttgg gaacaattgt atgttttctg
    21661 cactttgata tgacgctatt cctgttggta gcacaacatg cgttgttgct gtgttggtta
    21721 ttacagggtc attaaaatca aaataaatgg cagcagtgtt ttgtatgcta tcatttaata
    21781 ccaaacttgt tttgggtttt attctgtaat gaataaagcc atggcttaac ggctcgttaa
    21841 cattactatc gggcaataga atattatcaa acttaaattc catgttgcgc tcatgataaa
    21901 taaaattcat gtttacagga tgcgatgatg caacatattc taaggtgttt aactgaagtt
    21961 tatttgtatc taatggattt aaaattttaa cgttaaatgc tgtatcgttt cctgtgtttt
    22021 gaaaacggat gatgtaatct aaatagggtg gattgggaat ttctgtagtg aataaagtat
    22081 ctctacttac taaaatatca ttggggtcaa atgaacctgt ggtaaaaacc tcccaagaac
    22141 tgtaattaca accgggatta gcatcaccgg caacaggctc tattcttaca cttgaattga
    22201 taagtgaacc aatgggcaaa cccgtatgta catttactgt aacaacaata ttacctgact
    22261 gaaatggtgc aagcgagc
//
LOCUS       DAOOOL010000031        22270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_181_length_22270_cov_117.889219, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000031 DAOOOL010000000
VERSION     DAOOOL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22270
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181_length_22270_cov_117.889219"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1398)
                     /locus_tag="PKU82_04240"
     CDS             complement(67..1398)
                     /locus_tag="PKU82_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOZ82117.1"
                     /translation="MRKYLLILAYFFLISMTASAQWSTATVQSARSLLAATSIQDKAM
                     FGGGIGMISVPPPALDIYTQSTNQWQQSQISQGRTSLAAASTGNWAIFGGGANDWILS
                     TYNRVDIYNGTVGSWTNGDFLSVNRAKLAAAGAGSKILFAGGEFDMINAVYDTVDIFD
                     TLTQSWTTSALSVARFNLAGVSAGNKILFAGGENFSGNTYDNVDIYDVTTGTWTTANL
                     SVPRSRLAATSMGNKIYFGGGINNIGVVSNVVDIYDVVSNTWTTDTLSQARQYLTAAA
                     TMGKVFFAGGTDAGLATGSNVVDIFDTATATWSIDSLTTGRYFCAATALGTQFLISGG
                     LPDNSGFPINTVEIYTITSSGLAPIETSKTLSVYPNPASQKVVIKNNGFQPGDVINIS
                     DVMGKQVLSLKISKNETVADISSLSNGIYFVQLSSVSENFTSKSQKIIIQH"
     gene            complement(1583..2800)
                     /locus_tag="PKU82_04245"
     CDS             complement(1583..2800)
                     /locus_tag="PKU82_04245"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOZ82118.1"
                     /translation="MRNLDTLKLIFTLVIVGNLSMNITKAQTWTTQTSNSTEDLRAVN
                     FYDLNTGIASGINGAIVTTTDGGSTWVPRTSGVSTVLLSVFMADALTAYAVGMNGTIV
                     KTSDGGTTWTAQTSGTTKSLYNVFFVSPLTGWAVGETGLIKATTDGGTTWTTQTSGVT
                     TTLNALCFTSATEGWITGNGTGTSNTVLHTADAGVTWQPQTAPAGSYYDISFINSNEG
                     WIIGPYGAFANTTDGGTTWTDIAFNYSFQAWGVQFVSSTKGWMQVGSATNQNNSIKTT
                     NDGGISWQGSPSPVNVVADDIFFINANTGWAVGNDGIILKYSSGTGIDNFDDNSAISV
                     YPNPTKDFLNIENIESNTYVELCSILGNTIIAQKNNNSEKMQLDIRNLSKGLYFLKIE
                     NNRNKSIRTIVLQ"
     gene            complement(3002..8800)
                     /locus_tag="PKU82_04250"
     CDS             complement(3002..8800)
                     /locus_tag="PKU82_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOZ82119.1"
                     /translation="MTTKIAKTKRLFHLSRITFFLLCLFITGLAKAETEPNDSKAQAN
                     TLTLNTGMPGTVALSESDWFSVTTPEDGNLNITGTFSSYIYIQVYDNDGTTNLFLNYH
                     SGTVTRTVYGLAAGTYYVRLYNYTATETNTYTITPSLTATGFANDTEDNSTPALANVL
                     PLNGSVTGHVGYYYNNQRDTIDWYAVTVTQDGTLSYTITSENGLYIYAKLYDGDGTTQ
                     LAGNYTGSTATFSRGDMAPGVYYILIHAYYPNSEYVPYTLSNTLIPPVSANDPLPNET
                     FSTATPLALNGSTTGHIGYRYNGNVDVYDWYSVNTNADGDLYFTITSDNGQYVYAELF
                     DGDGITNLAGNFTTSTATYHKDGLAPGTYYIRIRTFYSYEYTSYQLSDSLGLPIYAND
                     ATPNETYSAATVLPLNDTITGHIGYRYNGNIDAVDWYSVTTNADGRLYWTITVDNGQN
                     VYAELFDGDGTTNLGGSYTTTTATFNANGLAPGMYYIRIKTFYAYQFASYQLSNSLVE
                     PIQANDALPNDIYTTATPLTLNDSTTGHIGYRYNGSVDVYDWYAVTTNADGQLFWTIT
                     SHNGQYVYAHLFDGDGITLLAGNYTSTTATFNENGLAAGTYYIRIKTYYGYEFAPYTL
                     SNSLTPALYTNDTEPNGSVATAVPLAVNTTVTGHSGYYYNNLDDTLDFYAITLPVDGK
                     LTWTITSGNGQYVYAQLYDGDGTTYLGGSYTNSTATYTKNNLAAGTYYIRIKTYYNNE
                     FTPYTLTNVLDPMNFAAENSADNAYAISPTLLPANTATTGHINFYYYGVNDTRDWWVM
                     GYDGTGAMTITCELEQNHFDVNYPDIAYAVYTDTAAAPIYSGTWINPTNTLTIPSPPI
                     SKYYLRLTQSANTFGAYRLTAAYTENCANNITSTSSQIVPCLGTINYTVSGGLADYTV
                     QLYKDGNPFGAPQTTSSTVSFTNLGIGTYYLQSHSFGASASCNNVSGNTVFSAPPVPN
                     ITPGGPTTFCAGGNVVLTADAASSYLWSTGATTQAITVSASGNYVVTVYNAALCSNTS
                     ASTSVTVNPLPPTPTITPNGPLTFCANSNVTLSSSVATGYLWSNASTTQSIVVNTAGT
                     FTVTINDANGCTASSASVTTNTIPLQTYYRDLDGDTYGDLGNTIQDCSPPAGYVSNST
                     DCNDYDIALHPGATELCNGIDDNCDSQVDEGVLSTFYADADGDGYGDLTVTTQACSAP
                     IGYVSNSTDCDDNNNAVHPGATEICNGIDDNCDGQIDEGCGSYTWYADTDGDTYGDPG
                     NFIVNYNPTPPAGYVADNTDCNDANNSIHPGATEIACNGIDENCNGMADDLGTPSTDP
                     SSITSNAVFDEICLGGNAILTVNGGSLGTGASWQWYSGSCGGTPVGTGSSITVSPSSA
                     TNYFVRAEGACGNSACVNLTITVKTSLPAHAVVIPPISGLPAYACPGSSASISIPAVP
                     NASQYIWDGPTGTYFNGNPLNTSPFTSATPNVQITFGTTNSSFYNIGVQAANSCGATL
                     RKIQKVRYKISTPASISGAVNACANSSSTYSIVAVTDATQYLWSITGDASVVGNGTTV
                     TVNFGPAWAGGNLCVAAQTPCFTSPAKCMYISSSATALNAISGTFTACPGSVITYSVP
                     PSAGASYYTWTLPAGASGSSNTNSIVVTFGPSYNTVDDICVTVTSVCGVTSAPKCKTV
                     APGLPARPNAITGPSNGLCAQTAPYSVSSSLGITYNWTAPGSISGNGTSAITVSYGSF
                     TTGSVCVTASNACGTSAARCITIKGAPNTPVSLTATPATWCANTQGIQFDADMSNTTG
                     AYTLNWTFPNAPTASYVSGGGNSNSLTLDWGTGNGTVIIKASNSCGEGSKAYVANIGC
                     KEAQQVDAGSIAAYPNPTAGMLNVEFNAVEKGNASVSVIDLSGRTILNSSQNVSAGNN
                     TMQLDLTKVAKGAYMISVKSNQFAKQIKIVVE"
     gene            9188..9331
                     /locus_tag="PKU82_04255"
     CDS             9188..9331
                     /locus_tag="PKU82_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOZ82120.1"
                     /translation="MGFKAEITDISTLTVRKHIANIYNKLHVNSKAQVISMAHKNKWF
                     NIF"
     gene            complement(9334..9963)
                     /locus_tag="PKU82_04260"
     CDS             complement(9334..9963)
                     /locus_tag="PKU82_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOZ82121.1"
                     /translation="MTTRNHQVYAELTPNPNSVKFVADRLLLFEGTVEYKSKAEAVDC
                     PIAYQLFDFTGIKSVFITTNFITITKEADIDWYEITNILREFIKGFLNSGEKLFLKNP
                     FPEQAETKEVIASKAEELVKQPVTQNPTEHSELEEKIITMLDEYVRPAVEGDGGAIDF
                     KSFKDGVVTVVLKGSCSGCPSSTLTLKAGIENLLKRMIPEVSEVMAEAE"
     gene            10040..11596
                     /gene="nadB"
                     /locus_tag="PKU82_04265"
     CDS             10040..11596
                     /gene="nadB"
                     /locus_tag="PKU82_04265"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOZ82122.1"
                     /translation="MTKTDFLVIGSGIAGLSFALKAAKHGKVTIITKANEDESNTKYA
                     QGGIAAVLPEAKDDSLEHHVQDTLSCGAGLCDEKIVRMVVAESGERINELIAWGTEFD
                     KNKSGNFDLAMEGGHSAHRVLHHKDITGLEIETKLLQQIHQHPNITLHTHLFAVDIIT
                     QHHMGVIVKRNTPGIACYGAYILNTKTGKVETMLAKVTVMATGGAGHVYKTTTNPVIA
                     TGDGIAMTYRAMGKVNGMEFIQFHPTALYHPGTYPSFLISEAVRGFGAVLRTIDGKEF
                     MQRYDERGSLAPRDIVARAIDSEMKKSGDDYVWLDCTKLNITEFKKHFPNIYNRCMSL
                     GIDVAKDCIPVAPAQHYLCGGIKVDEHGQSSIQHLYAIGECSSTGLHGGNRLASNSLL
                     EAVVFAHRAYEHASERLEFIHYNEHIPEWNSVSPREAAEMVLITQSRKELQEIMSSYV
                     GIFRSDMRLKRALERTRLLYEETEDLYNRWEISPRICELRNLINVAYLIIKAAMARTE
                     NKGLHFNVDN"
     gene            11612..12577
                     /locus_tag="PKU82_04270"
     CDS             11612..12577
                     /locus_tag="PKU82_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HOZ82123.1"
                     /translation="MISIFENNFNFENTALESFRFQAENNAVYRNYLKLLNIKPNQIS
                     NVSQIPFLPISFFKTKKVVSGNNDSKAIVFSSSRTTGAEPSLHYVNDISLYEKAFTQT
                     FNQYYSSADQWCILALLPSYLEREGSSLVYMCQHLIERTGKKNSGFYLNDFRKLADTI
                     IVNEQKGIKTLLIGVSFALLDFAHTHPMPLKNTIIMETGGMKGRRKEMIRQELHTILM
                     EAFQLNAIHSEYGMTELLSQAYSSGNGIFKCPPWMKVFVRDSNDPLSESKPGQGGINI
                     IDLANINSCSFIATQDIGRVHADGTFEILGRFDNAEVRGCNLLIE"
     gene            12591..12938
                     /locus_tag="PKU82_04275"
     CDS             12591..12938
                     /locus_tag="PKU82_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOZ82124.1"
                     /translation="MEKVTNLDFIKGFSKGDMSRVKKYVMMYIQSADEEMPVIEKSFN
                     DKDYKKLKTSSHTLKSIAGYMGMDHAQQLLKKIEDSASNESVTEEMSHDIAQLKEMIT
                     LSKDELKAFIAQN"
     gene            12975..13493
                     /locus_tag="PKU82_04280"
     CDS             12975..13493
                     /locus_tag="PKU82_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ82125.1"
                     /translation="MSKSSTIFIIDDDPMMQSMLKDHLSAMSNFTIHTFATGEEALAS
                     GQLVPDIVFLDYNLDSKIANAMDGIDVLREIKSKYPATEVVMLSGQDKIEVAVDTMKY
                     GAFDYIVKGESAFYRAEKTVFNLFKLAQLKKNAGMYKILTITFGAALLLMIILVIILK
                     AKGMITDHVGWY"
     gene            13506..13991
                     /locus_tag="PKU82_04285"
     CDS             13506..13991
                     /locus_tag="PKU82_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82126.1"
                     /translation="MKTVLALLLIFSLTSCRKEEKYPIEPVLLYKSVQFYEKNSFYLT
                     TTFTDGDGDIGLSDADTLPPYDQTSHFYHNYFCNYQQLINGSWTPINFSIPYYYRIPM
                     LTPKQRNKNIKGDIQVDVKNFLSFTTNGIADTFRVEISVADRALHESNKVLSDAFVIY
                     K"
     gene            complement(14033..16801)
                     /gene="leuS"
                     /locus_tag="PKU82_04290"
     CDS             complement(14033..16801)
                     /gene="leuS"
                     /locus_tag="PKU82_04290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOZ82127.1"
                     /translation="MDYQFRETEKKWQQYWAANKTFKVENKSDKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGYDSFGLPAEQYAIQTGQHPAITTET
                     NINRYREQLDKMGFSFDWDREVRTSDPSYYKWTQWIFLKLFDAWYNNKSNRAESIDKL
                     ISEFEKNGNNDISAVHDEIENFTAAQWKNFDETEKSRILMAYRIAYLGEAYVNWCPAL
                     GTVLANDEVKDGLSERGGHPVERKLMKQWSMRITAYAERLLQGLENIDWSDSLKEAQR
                     NWIGKSEGTTIRFSLQNSDDYIEVFTTRPDTLFGVSFVTLAPEHELVDKITTSSQRAE
                     VQDYVTKTKNRSERERQAEVTRISGVFTGSYVIHPFTGNKIPVWIGDYVLAGYGTGAV
                     MAVPAHDERDFRFATHLGLDRPQVIEPNITHDFGKGSWDAKEGKLINSGFLNDLDVKS
                     AISCCITEIEKQNLGKGKTNYRLRDAVFGRQRYWGEPIPIYYKENIPVAIPENELPLV
                     LPEVDKFLPTETGEPPLARAKNWKYKNLFPLETTTMPGWAGSSWYFLRYMDANNNTSF
                     ASKDALNYWQNVDLYIGGSEHATGHLLYVRFWTKALKDLGYINIDEPAQKLINQGMIQ
                     GTSCMVHREVATNKIISVGLKSNYNTTLIHIDVNLVDNTEVDIEGLKKWRDDFATAEF
                     VTENGKLICETAVEKMSKRWYNVVTPDSICEKYGADCLRMYEMFLGPLEQSKPWNTNG
                     IAGVSNFIRKLWKLYHNNGKFEVSNETATKAELKTLHKTIKKVQEDVEKFSFNTSVST
                     FMICVNELTEHKCNKREVLEPLAIILSPYAPYIAEELWQLLGHSNSVSFATFPEWNET
                     YLIENEFDYPVSVNGKTRFKIQLPLSIDKEEIERHVMASEEMQKWTNGNVPKKVIVVP
                     GRIVNVVI"
     gene            complement(16860..17630)
                     /locus_tag="PKU82_04295"
     CDS             complement(16860..17630)
                     /locus_tag="PKU82_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ82128.1"
                     /translation="MSLDLFYRSSGNGQPLIILHGLFGLNDNWQSFSKQITENNIAVY
                     AVDLRNHGSSPHDSVFDYDSMADDVFAFISKHDLQNVNIMGHSLGGKVAMKFALKHQD
                     ILKSLIVVDIAPRYYAPHHQQIIKALQAVDLDTVNTRKEAEHILSGYIDDAGVKMFLL
                     KNLYWEGDRLQWRFNLKVIAEKIESVGERITSTEKFEGKTLFIKGEKSKYITDDDKSE
                     ILNLFPEAEIKTCANAGHWVHADNPECMLTTVRDFLSQ"
     gene            complement(17630..20071)
                     /gene="pheT"
                     /locus_tag="PKU82_04300"
     CDS             complement(17630..20071)
                     /gene="pheT"
                     /locus_tag="PKU82_04300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOZ82129.1"
                     /translation="MKISLDWLKDYIKLQQTPEEIEHLLTSSGLEVESLDETDSIKGG
                     LRGLVVGEVLEKEKHPDADRLSVTKVNVGKTIPLQIVCGAANVAKGQKVLVALNGARL
                     YPTFGEPFDIKNSKIRGQASEGMICSEDEIGLGNSHEGIMVLDANAVPGTPAAEWFKI
                     KQDHIFEIGLTPNRIDAASHGGVARDLAAVINTRAKAENNNSTTAQFSMPDVGAFKSE
                     NNALRIDVVVDKNSGCGRYCGVSISGVTVGESPEWLKSRLQSIGVKCINNIVDVTNFV
                     MFETGQPLHAFDAARVKGKIIVQKTGKASVFTTLDHIERKLTGDEVMICNATEPLCIA
                     GVMGGTDSGVSASTTQIFLESAWFNAAEVRKAAKHHGLKTESSFRFERGTDPNGTLYA
                     LKRAALLIKEVAGGKITSDVVDLYPEVVEPRIIYVNFEKINKLSGINFSNDMLLSVLE
                     YCGFSIKSSNVNGATISIPTYKTDVTRQADVVEEILRTYGYHHIPLNEKTGISFSSHT
                     PDRKPAMQYKISHHLSSIGFFEMINNSLVSSSHSSAGDEAVKVLNPLSKELDILRPDM
                     VASTLHTICYNINRKNHNLKLFEFGKTYLQHEGKLKETNNLVIAMTGMVNEPNWHKSE
                     KESSLFYLKSVAEKAIELVMPRISSHLNWVQDSHTLFSTFANIMQRQSAFGYIGSVKK
                     NILKQFDIAQPVYLAVLNIDVLLEKSLSHKTAIKLPQPFPEVKRDLSLMLDQAINYAD
                     LRDLALKQEKKLLKQVDIFDVYEGEKIEAGKKSYALSFTLADDEATLTDKQIESVMQK
                     IEKAFEKNLGAVIRK"
     gene            complement(20146..>22270)
                     /locus_tag="PKU82_04305"
     CDS             complement(20146..>22270)
                     /locus_tag="PKU82_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ82130.1"
                     /translation="NMGKFGELPTFEELENPNSSLASEVISSDNVLLGKYYIQNRSNI
                     HYKELAENTVDALKATEDIRFEEHSGVDMRGLGRVFFKTVILQQRSSGGGSTITQQLA
                     KNLFPRENESGFRLAVRKVKEWIIAVRLERNYTKQEIMAMYLNTVEFGHNSYGIKSAA
                     KTFFNKMPSQLKVEESAMLVGLLQAPTRYSPVRNPKNALIRRNTVLSQMEKYKFLSEA
                     AYDSIVKLPIKLNFRAEDHNDGLAPYFRETLRLELVKWCKEHKKSDGSSYNLYRDGLR
                     IYTTIDSRLQEYAEEGMKEHLTELQKTFYQHWKGRVPWAQHPEVIEDGIKKSDRYISL
                     KAEGLSEQEIKENFATKIPMTIFSWKGDIDTMMSPLDSLKYYKMFLQAGVMSIEPQTG
                     YVRAWVGGNNYRYFKYDHVKAGRRQVGSTFKPFLYTLAMQEGYSPCFKVPNVRVTIPI
                     PGQPDWSPSNSDGKYGGMLTLKEALAESVNSVSAYLMKQFGPKPMIEIARKMGITSPI
                     DEVPSICLGTPDVSLFEMCGAYSTFANKGVWTEPIYITRIEDKNGMVLQEFVPRKVEA
                     ISEETAYLMLNLMQGVVQSGTGGRLRFRFGLTNPIAGKTGTTQNQSDGWFMGITPTLV
                     TGVWVGCEDRSVHFRTTSLGQGANTALPIWALYMKKAYADKDLNLYNGDFEKPEHPLS
                     IETDCSKYEQDKKDHQHNSGFGSDL"
BASE COUNT         6721 a         4452 c         4151 g         6946 t
ORIGIN      
        1 cttatataaa agattaatat tgaaaatatg ctgccaaaca gaaattaaaa ttcaacattt
       61 aaaattttag tgctgaataa taattttttg tgattttgat gtaaagtttt ctgaaactga
      121 actaagttga acaaaataaa ttccattact caggctgcta atatctgcaa cagtttcgtt
      181 ttttgaaatt ttcaagctaa gaacttgctt ccccattaca tcagatatgt ttattacatc
      241 accgggttgg aagccattat ttttgattac aactttttgt gatgcagggt ttggataaac
      301 acttaatgtt tttgaagttt caataggtgc aagtccactt gaagtgatag tataaatttc
      361 cactgtgttg attgggaaac ctgagttgtc aggcaatcca ccactaatta aaaactgcgt
      421 tcctaatgca gttgcagcac aaaaataacg tcctgttgtc agagaatcaa tactccaagt
      481 tgcagtggca gtatcaaaaa tatcaacaac atttgaacct gttgccaagc ccgcatctgt
      541 tccacccgca aagaatactt ttcccatagt ggcagctgcg gttaaatatt gtcttgcctg
      601 tgacagtgtg tcagtagtcc aggtattgga tacgacatca taaatatcta ctacattact
      661 caccactcca atattgttaa tgcctccacc aaaataaatt ttattaccca ttgatgttgc
      721 agctaatctt gaacgtggta ctgataaatt tgctgtagtc catgttcctg tagttacatc
      781 ataaatatca acattatcat aagtgtttcc tgaaaaattt tcaccaccgg caaacagaat
      841 cttattgccg gcagatacac ctgcaagatt aaatcgtgca acactcaatg cacttgtggt
      901 ccaactctgc gtaagcgtat caaaaatatc aaccgtatca taaacagcat taatcatatc
      961 aaactcacca ccggcaaaaa gaattttgct ccctgcacct gcagccgcta atttagcacg
     1021 attaacagaa agaaaatctc cattagtcca actgcctaca gtgccattat aaatatcaac
     1081 tctgttatag gtagaaagaa tccagtcgtt ggcaccacca ccaaaaattg cccagttgcc
     1141 tgttgacgct gctgctaatg aagttcgtcc ttgtgaaatt tgtgactgtt gccattgatt
     1201 tgttgactgt gtgtagatat ctaaggcagg tggtggaaca cttatcatac caataccacc
     1261 accaaacata gctttatctt gaatagaagt agctgcaagt aaactacgtg cagattgaac
     1321 agtagctgta ctccattgtg ctgatgcagt cattgaaatt aaaaagaagt aagctaaaat
     1381 gagtagatat tttctcataa aattccgatt gaatattttt acaatattag taaaaataat
     1441 tttcagagaa tcttttacaa tagtaataat tgggtagtaa aatcctgata tgaaaatctc
     1501 tttagctttt aattcagcta aaaaatttta taaattcaag gtgctaaatc aaagcagctg
     1561 taaagaatat tcagataaaa aattattgta agacaatagt tcttattgat ttgtttctgt
     1621 tattttcaat ctttaaaaag tacaaaccct tagataaatt gcgtatatct aactgcattt
     1681 tttccgaatt gttatttttc tgggcaataa ttgtatttcc taaaatacta cataattcta
     1741 catatgtatt actttcaata ttttcaatgt ttagaaaatc ttttgttgga tttggataaa
     1801 cacttattgc agagttatca tcaaaattgt caatacctgt tccggaacta tattttagaa
     1861 tgataccatc attaccaact gcccagcctg tatttgcatt aataaagaat atatcatcag
     1921 caacaacgtt gaccggtgat ggagaacctt gccatgaaat accgccatca ttagttgttt
     1981 ttatggaatt attttgattt gttgcagaac ccacttgcat ccatcctttt gttgatgaaa
     2041 caaattgaac accccatgcc tgaaaactat aattgaaagc tatatccgtc catgtagttc
     2101 caccatcggt agtatttgca aaagctccat aggggccaat tatccaacct tcgttagagt
     2161 taataaaact tatatcataa taactcccag ccggggcagt ttgtggttgc catgttaccc
     2221 ctgcgtcagc ggtatggagc acagtattac tagttccggt accattgcca gttatccaac
     2281 cctctgttgc agaagtaaaa cataatgcat tcaaagtagt tgttacacct gaagtttgag
     2341 ttgtccatgt agttccacca tcagtagttg cttttatcaa ccctgtttct ccaacagccc
     2401 aaccggttaa tggacttaca aaaaacacat tgtacaacga ttttgttgtg cctgaagttt
     2461 gagctgtcca tgttgtacca ccgtcagatg ttttaacgat tgtaccattc attcccacag
     2521 catatgcagt aagtgcatct gccataaata cacttaataa aacagtactt acacctgaag
     2581 ttcgaggtac ccaggttgat cctccatctg ttgtagtaac tatggctcca ttaataccgg
     2641 aggcaatacc tgtatttaaa tcatagaagt ttacagctct taaatcttca gttgaattgc
     2701 tggtttgagt tgtccatgtt tgtgcttttg taatattcat tgataagtta ccaacaatta
     2761 ccaatgtgaa aattaatttt aatgtgtcta aatttctcat attctttggt ttaaatttta
     2821 aaaacaattg tgacccgaaa tatcggaatt attttttaca aataaatatt tatcaaaaag
     2881 tttctgcaat caccaattct ggtgattttt ccggttaata atggtgtagt taagttcgta
     2941 aaacaaaaac aaggctgccc tttgggcagc cttgttgcaa aacaattaga atgctaaatt
     3001 attactcaac aacaatttta atttgtttag caaactgatt tgattttaca ctaatcatgt
     3061 aagcaccttt agctactttg gttaaatcga gctgcatagt attatttcct gcagaaacgt
     3121 tctgagatga attcaggatt gttctgccac ttaaatcaat tactgaaact gatgcattgc
     3181 ccttttcaac tgcattgaat tcaacattca acataccggc agttggatta ggatatgcag
     3241 ctatagaacc tgcatcaact tgttgtgctt ctttacaacc aatgttcgct acgtaggctt
     3301 tagaaccttc accacaggag ttgcttgctt taataataac agttccatta cctgtgcccc
     3361 aatcaagagt taatgagttg gagtttccac cacctgaaac ataacttgca gtaggcgcat
     3421 taggaaatgt ccagtttaaa gtataagcac ctgtagtatt actcatgtca gcatcgaact
     3481 gaataccttg tgtgtttgca caccatgttg caggggttgc agttaacgaa acaggcgtat
     3541 taggtgctcc tttgattgta atgcaacgag cagcacttgt gccgcaggca ttgctggcag
     3601 taacacaaac agagcctgta gtaaaagagc cgtagctaac ggtaatggca ctggtgccgt
     3661 tgcctgaaat agaaccaggt gcagtccaat tgtaagtaat gccgagcgaa gaactaacac
     3721 tgtaaggagc cgtttgtgca cacagaccgt tagaaggacc ggtgatagca ttaggtcttg
     3781 ctggtagtcc gggagcaaca gttttgcatt taggcgcaga agtaactcca caaacacttg
     3841 taacggtaac acatatatca tctacagtgt tataagatgg gccaaaggtt acaacaatac
     3901 tgttggtatt agaactacca gaagcaccgg caggaagagt ccatgtatag taagaagcac
     3961 cagcagaagg aggcacactg taagtgatca cactaccggg acatgctgta aatgttccgc
     4021 tgatggcgtt aagagcagta gcagaagaac tgatgtacat acattttgcc ggagaagtaa
     4081 agcaaggagt ttgagcggca acacaaagat tgccaccagc ccatgcggga ccaaaattaa
     4141 cagtgactgt tgtaccatta ccaacaactg aggcatcacc ggtaatgctc cacagatatt
     4201 gtgtagcatc agttactgca acaatagaat atgtgctact gctgttggca catgcattaa
     4261 cagcaccaga gattgaagca ggggtactga ttttataacg taccttttga attttacgaa
     4321 gtgttgcacc gcaactgttt gcggcttgta cacctatgtt atagaaggag gagtttgtgg
     4381 taccaaaagt tatctgcacg tttggagttg ctgaagtgaa tggtgaagtg tttaacggat
     4441 taccgttaaa gtaggttccg gtaggtccat cccatatata ctgagaagca ttaggtacag
     4501 caggaatact tattgaagca gaagagccgg gacatgcata agcaggtaag cctgaaatag
     4561 gaggaataac tacggcatgt gcaggcagag aggtttttac agtgatagtg agattaacac
     4621 atgcagaatt accacaagca ccttcagcac gaacgaaata attagtagct gaagaaggag
     4681 aaacagtaat actgctacct gttccaacag gagtgccacc acaagagcca ctgtaccact
     4741 gccatgaagc accggtgcca agactaccac catttacagt taaaattgca ttaccaccta
     4801 aacagatttc atcgaataca gcattgcttg taatggaaga tggatcagta cttggtgtac
     4861 ctaaatcatc agccatgcca ttacaatttt catcaatacc gttgcatgca atttcagtag
     4921 caccaggatg aatagaatta ttggcatcat tacaatcggt gttatcagca acataacctg
     4981 caggaggagt aggattataa tttacaataa aattgccagg atcaccatag gtgtcaccat
     5041 cagtatcggc ataccatgta taactgccac aaccttcgtc aatctgacca tcgcagttat
     5101 catcaatgcc attgcatatt tcggttgcac cgggatgaac agcattatta ttatcatcac
     5161 aatctgtact gttgctaaca tatcctatag gagcagaaca agcctgagta gtaactgtta
     5221 aatcgccata accatcgcca tctgcatcgg catagaaagt tgaaagaaca ccttcgtcaa
     5281 cctgactatc gcagttatca tcaataccgt tacaaagttc cgtagcacca gggtgaagag
     5341 caatatcata gtcgttacaa tcggtgctgt tagaaacata gcctgctggt ggagaacaat
     5401 cttgaatagt attaccaaga tcaccataag tatcaccatc aagatcgcga tagtaggttt
     5461 gcagaggaat ggtgtttgtt gtaacagatg ctgatgaggc agtacagcca ttggcatcat
     5521 ttatagttac cgtaaatgtg ccggcagtgt taacaacaat actttgtgta gtagaagcat
     5581 tgctccataa atagccagtg gctacacttg aagatagcgt tacattgctg tttgcacaga
     5641 aagtaagcgg accattaggt gtaatggttg gtgttggagg caatgggtta acagttacag
     5701 aagttgatgc agaagtattg ctgcacaatg ctgcattgta aactgtaacc acataattac
     5761 ctgatgcact tactgtaatg gcttgtgtag ttgcaccggt actccagaga taagatgaag
     5821 cagcatcggc agttaaaaca acatttccac ctgcacagaa ggtagtagga ccaccaggag
     5881 tgatgtttgg aaccggaggt gcagagaata ctgtgttacc acttacatta ttgcaagaag
     5941 cagaagcacc gaaggaatgt gattggagat aataagttcc aataccaaga tttgtgaagc
     6001 taacagtaga agaagttgtt tgtggtgcac caaatggatt accatcttta taaagctgaa
     6061 cagtataatc tgctaaacca cctgaaacag tatagttaat ggtgccaagg caaggtacta
     6121 tttgcgaaga ggtgctggta atattgtttg cacaattttc tgtataagca gctgttaaac
     6181 gataagcacc aaaggtgttt gcagactgtg tcagtcgcaa ataatatttg cttatgggtg
     6241 gagaaggaat tgttaatgta tttgtgggat taatccaagt gccggaatat attggagcag
     6301 cagccgtatc agtataaact gcataagcaa tatcaggata attaacatca aaatgatttt
     6361 gctccaattc gcaggtaata gtcattgctc ctgtgccatc ataacccatc acccaccagt
     6421 cgcgtgtgtc attaactccg tagtaataga agttgatgtg acctgttgtt gctgtgttgg
     6481 caggtagcag tgttggagaa atggcatagg cattatcagc agaattttct gctgcaaaat
     6541 tcatcggatc caatacattt gttaatgtat atggtgtaaa ctcgttgttg taataagttt
     6601 ttattcttat atagtatgtg cctgctgcca gattattttt agtgtatgta gcagttgaat
     6661 ttgtataact accacctaag taagttgtac catcaccatc ataaagttga gcatacacat
     6721 attgtccgtt gcctgaagtt attgtccagg taagtttacc atctacagga agtgtaatag
     6781 cataaaaatc taaagtatca tctaaattgt tataatagta accactgtgt ccggttacag
     6841 ttgtattaac tgctaatggg actgccgtag caacactgcc gtttggttct gtatcattag
     6901 tataaagcgc aggcgtaagt gaattgctta aagtatatgg tgcaaattca tatccatagt
     6961 atgttttaat tcttatataa taagtgccgg cagcaagtcc gttttcatta aaagtggcag
     7021 tagttgatgt atagtttccg gctagcaatg ttattccatc tccatcaaaa agatgtgcat
     7081 aaacatattg tccgttatgc gaagttattg tccagaatag ttgaccgtct gcattggtag
     7141 ttacagcata ccaatcataa acatcaacac ttccgttata gcgataacca atatgaccgg
     7201 tagttgagtc atttagtgta agtggtgtag ctgttgtata aatgtcattt ggcaaagcat
     7261 catttgcctg aataggttca actaaactat ttgatagctg ataggatgca aattgatagg
     7321 catagaaagt cttaatacga atgtagtaca tgcccggtgc cagaccattt gcattgaaag
     7381 tggctgttgt ggtcgtataa cttccaccta agttagtagt accatcacca tcaaatagtt
     7441 ctgcataaac attttgaccg ttatcaactg taatagtcca atataaacgt ccatcagcat
     7501 tggtagtcac actgtaccag tcaacagcat caatatttcc gttataacga tatccaatgt
     7561 ggcctgtaat tgtatcgttc aaggggagta cagttgctgc tgagtaagtt tcattcggtg
     7621 ttgcatcatt agcataaatc ggcaaaccta aactgtcaga taattgataa gaagtatatt
     7681 cataagagta aaaagttctg atacgaatat aataagtgcc cggtgctaaa ccatctttat
     7741 gatatgttgc agtagatgtt gtaaagtttc cggctaagtt tgtaatgcca tcaccatcga
     7801 ataattctgc gtaaacatac tggccattat cactggttat agtaaagtat aagtctccat
     7861 cagcatttgt attaacacta taccagtcat aaacgtcaac atttccgttg tagcgatatc
     7921 caatatgacc tgttgtactg ccatttaaag caagcggtgt tgcagttgaa aaagtttcat
     7981 ttggtaaggg gtcatttgca ctaacaggag gtattaatgt attggatagt gtatatggta
     8041 catattcact gttagggtag tatgcatgaa ttaatatata atatactcca ggcgccatat
     8101 cacctctgga gaaagttgca gtacttcctg tataatttcc tgcaagttgg gttgtgccat
     8161 caccatcata aagtttagca taaatgtata aaccattttc acttgtaata gtgtagctta
     8221 aagtgccatc ctgagtaacg gtaacagcat accagtctat agtatcgcgt tggttgttgt
     8281 aatagtagcc aacatgtccg gtaacacttc cattcagagg taacacattt gctaatgcag
     8341 gagtgctgtt atcctctgtg tcgttggcaa agcccgttgc tgtcaggctt ggtgttatcg
     8401 tataagtgtt ggtttccgta gcagtgtaat tgtatagtcg cacataatag gtgcctgctg
     8461 ccaagccata aactgttctg gttacggttc cagaatgata attgagaaag agattagttg
     8521 taccatcatt atcgtaaacc tgaatataaa tgtaactcga aaaagtaccg gtgatattca
     8581 gatttccatc ttccggagtg gtaacagaaa accagtcaga ttcactgagt gcaacagtac
     8641 cgggcattcc tgtatttaat gttaatgtgt tggcctgagc cttgctgtca ttaggctctg
     8701 tttcggcttt tgctaagcct gtaatgaaca agcatagcaa gaaaaaggtt attcttgata
     8761 agtgaaacaa cctttttgtt tttgcgattt tagttgtcat aataatttgg tttttaagtg
     8821 tgaaataatt tattttctat tttgcaataa tgtgactttt taagtatgct gtcaatcgcc
     8881 aattctggtg atttttttaa ttcagaaagc tacgtattct tatttgcgtc ttttgaaccg
     8941 agaattatta gattatttta atgctacaaa tatttctact aattaatttt attgtaaata
     9001 cttaatatgt agtaatcaga aaatatttca gatgaatgat actacaaatt gagtattgac
     9061 ttttttggct taaaaaaaag atcctgattt caatctgttt atttagtttt acggtattaa
     9121 ttattaaaaa ggcattcaga tgtttttctg ttttggtgtt tattttggca cagttgaata
     9181 atgcaatttg ggttttaaag ctgaaataac agacatcagc actttaactg ttagaaaaca
     9241 tattgcaaat atttacaaca aactgcatgt aaattctaag gcgcaggtaa tttcaatggc
     9301 gcataaaaac aaatggttta atatttttta gaactattcg gcttctgcca tcacttccga
     9361 aacttcagga atcattcgtt tcagcaaatt ttcaattcct gcttttaacg ttaatgttga
     9421 cgaaggacaa ccactgcatg agccttttaa aactacagta acaacaccat ctttaaacga
     9481 cttaaaatct atggcaccgc catcaccttc aacggcagga cgcacatatt catcaagcat
     9541 ggtgataatt ttttcttcca gttcagaatg ttcagttggg ttttgtgtca ccggttgctt
     9601 taccaattct tctgcttttg aggctattac ttcttttgtt tcagcttgct ccggaaaagg
     9661 atttttaaga aataattttt caccgctatt taaaaaccct ttaataaact ctcgcaagat
     9721 attggtgatt tcataccaat caatatcggc ttcctttgtt atagttataa aatttgtagt
     9781 tataaagaca gacttaatac cggtaaagtc aaacaactga taagctatcg gacaatctac
     9841 cgcttcggct ttagatttgt attcaacagt gccttcaaaa agcaacaacc tgtcggcaac
     9901 aaattttaca ctgttgggat taggtgttaa ctcggcatag acctgatgat ttcttgttgt
     9961 catgaatagt tacttttgca attaatttac gaaagtaaac atattcgact tattttagtt
    10021 taaaatttcg attaataaaa tgacaaagac cgattttctg gtgataggca gcggaatagc
    10081 cggcttaagt ttcgcattaa aggcggccaa gcatggcaaa gtaaccatca tcacaaaggc
    10141 taatgaagat gaatctaaca ccaaatatgc acaaggcggc attgcagccg ttttgccgga
    10201 agcaaaggac gactctttag aacatcatgt tcaggatacg ttgagttgtg gtgcaggatt
    10261 gtgtgatgag aagatagtgc gcatggtagt agccgaaagt ggcgaacgca taaatgaact
    10321 cattgcctgg ggaacagaat ttgacaagaa caagagtggc aactttgatt tagctatgga
    10381 gggtggtcat tccgctcatc gtgtattgca tcataaagac attaccggac ttgaaattga
    10441 aacaaagctg cttcaacaga tacatcagca tccaaacatc acattacata cccatctttt
    10501 tgctgttgac atcattacgc aacatcacat gggtgttatt gttaaacgta atactccggg
    10561 aatagcatgt tatggtgcat atattttaaa tactaaaacc ggcaaagtag aaactatgct
    10621 tgcaaaagtt acagtgatgg ctaccggtgg tgcaggacat gtttataaga ctacaaccaa
    10681 tcctgttatt gcaaccggtg atggcattgc catgacctat cgtgccatgg gaaaagtaaa
    10741 tggcatggag tttattcaat ttcatcccac agcattgtat catcccggaa cctatccatc
    10801 ttttctcatt tcagaggcag tgcgtggttt tggtgcagtg ttgcgaacta ttgatggcaa
    10861 agaatttatg caacgctatg atgagcgtgg gtcgcttgcg ccacgcgata ttgttgcccg
    10921 tgcaattgat agcgaaatga aaaaaagtgg tgacgattat gtttggcttg attgtacaaa
    10981 attaaatata acagagttta aaaagcattt cccgaatatt tacaaccgat gcatgtcgtt
    11041 gggcattgat gttgctaaag actgtattcc tgttgcaccg gcacaacatt atttatgtgg
    11101 aggtattaaa gttgatgagc atggacaatc gtctattcaa catctttatg caattggaga
    11161 atgttcgtca accggactgc atggtggaaa tcgtctggcg tcaaattcat tactcgaagc
    11221 tgttgtattt gcgcatcgtg cttatgagca tgcctcagag cgattggaat ttatccacta
    11281 caatgaacac attccggagt ggaattcagt ttcgccacgc gaagcagcag aaatggttct
    11341 tattacacaa agccgaaaag agttgcagga aataatgagc agctatgttg gaattttcag
    11401 aagtgatatg cgactaaaac gggcgcttga acgtacacga cttttatatg aagagacaga
    11461 agacctgtat aacagatggg aaatttctcc tcgtatatgc gaattgcgca acctcattaa
    11521 tgtagcatac ctgattatta aagcagcaat ggcaaggaca gaaaataaag gactgcattt
    11581 taatgtagat aattaaatta attgcttctg tgtgatttca atttttgaaa acaattttaa
    11641 ttttgaaaat acagcacttg aaagttttag gtttcaggca gagaataacg ctgtttatcg
    11701 caactatctg aaattattaa acatcaagcc aaatcagata agtaatgtta gtcagattcc
    11761 ttttctgcca ataagttttt ttaaaacaaa aaaagtagtc tcaggaaata atgactcaaa
    11821 agcaatagtt ttttcaagta gcagaactac aggtgcagag ccatcactac attatgtcaa
    11881 tgatatttct ttatacgaaa aggcttttac acaaacattt aatcagtatt attcgagtgc
    11941 agaccaatgg tgtatattag ctttattgcc ttcttatctg gagcgcgaag gttcatcgct
    12001 ggtgtatatg tgtcaacatc ttattgaaag aacaggaaag aagaacagtg gattttatct
    12061 taatgatttc agaaaattgg cagataccat tatagtaaat gaacaaaagg gaattaagac
    12121 tttacttatc ggtgtaagtt ttgcattgct tgactttgcc catacacatc cgatgccact
    12181 aaagaacaca attatcatgg aaacaggagg tatgaaagga cgaagaaaag aaatgataag
    12241 gcaagaactg catactatcc tgatggaggc gtttcagttg aatgcaattc attcagaata
    12301 tggaatgaca gaactgcttt cgcaggcata tagtagcggt aacggtattt ttaaatgccc
    12361 gccatggatg aaagtttttg tgcgcgacag caacgaccca ctgtcggaaa gcaaacccgg
    12421 acaaggtgga atcaacatta ttgatctggc aaatattaac agttgtagtt ttattgcaac
    12481 tcaagatatt ggaagggtcc atgctgacgg cactttcgaa attctcggca ggttcgataa
    12541 tgcagaagtg cggggatgca atcttttaat tgaataacta tttttgcagt atggaaaaag
    12601 ttaccaacct cgattttatt aaaggattca gcaaaggcga catgagccgc gttaagaagt
    12661 atgtcatgat gtatatacag agtgctgatg aggaaatgcc tgttattgaa aaatcattta
    12721 atgataagga ttataaaaaa ctcaaaacat cctcacatac cctaaaatca attgccggct
    12781 atatgggaat ggatcatgca cagcaactgt taaaaaaaat tgaagatagt gcatctaatg
    12841 agagtgtaac tgaagaaatg tctcatgata ttgcacaact taaggagatg ataactttat
    12901 caaaagatga actaaaagcg ttcatagctc aaaattaatt tgtattcttg tcatgctaat
    12961 actactgtca taatatgtca aaaagctcaa caatatttat cattgatgat gacccaatga
    13021 tgcaaagtat gcttaaagat catctgtcgg caatgtcaaa ctttaccatc cacacttttg
    13081 ccacaggtga agaggcattg gcttccggcc agttagttcc tgatattgta tttcttgatt
    13141 acaatctcga ttctaaaatt gctaatgcaa tggatggaat agatgtttta agagagataa
    13201 aatcaaaata tcctgccaca gaagttgtca tgctttcagg tcaggataaa attgaagttg
    13261 ctgttgacac catgaagtac ggtgcattcg actatattgt gaaaggtgaa agtgctttct
    13321 accgtgcaga aaaaacagtg ttcaatctat ttaagttagc acaactgaaa aagaatgccg
    13381 ggatgtacaa gattttaacc atcacttttg gtgctgcatt gttactcatg attattttag
    13441 tcatcattct taaagctaaa ggaatgatta ctgatcatgt tggctggtac taatatcaca
    13501 aaaccatgaa aactgttctt gcactcttat tgattttctc acttacatca tgccgtaagg
    13561 aggaaaaata tcctatagag cctgttttgt tatataagtc agtgcagttt tatgagaaaa
    13621 acagttttta tctcaccaca acttttacag atggcgatgg agatattggg ttaagcgatg
    13681 cagatacttt gccaccttat gaccagacaa gccatttcta tcataattat ttttgtaatt
    13741 atcagcaact catcaacggc agttggacgc caataaattt cagcattccc tattattacc
    13801 gcattccaat gcttacacca aaacagcgaa acaagaatat caaaggagat attcaggttg
    13861 atgttaaaaa ttttctaagt ttcacaacga atggaattgc cgacacattc agagtagaaa
    13921 tttctgttgc cgacagggca ctgcatgaaa gtaataaagt gctttcagat gcttttgtaa
    13981 tatataagta acaaaatatt tcgagggcgt aaatgcaggt taactcaaaa ggttaaatta
    14041 ccacattaac aattcttccc ggaacaacaa taactttctt tggtacattg ccatttgtcc
    14101 atttctgcat ttcttcagag gccatcacat gtctttctat ttcttcctta tcaatactta
    14161 aaggcaattg aattttaaat ctggtttttc catttacaga aacaggataa tcaaattcgt
    14221 tttctattaa gtacgtttca ttccattccg gaaaagttgc aaaagaaaca gagttactgt
    14281 ggccaagcag ttgccataat tcttctgcaa tgtatggcgc atagggtgaa agaattattg
    14341 ctaaaggttc aagtacttcg cgcttattgc atttatgttc tgtcagctca ttcacacaaa
    14401 tcataaatgt acttacagaa gtgttgaatg aaaatttctc aacatcttcc tgaacttttt
    14461 ttatagtttt atgaagcgtt tttaattctg cctttgtagc ggtttcatta cttacttcaa
    14521 atttaccatt gttgtgatac agtttccaaa gctttcttat aaaatttgat acgcctgcaa
    14581 taccattggt gttccatggt ttgctttgtt caagaggtcc cagaaacatt tcatacattc
    14641 gtaagcaatc ggcaccgtat ttttcacaaa tcgagtctgg tgttacgaca ttataccaac
    14701 gtttactcat tttttcaacg gcagtttcgc aaatcagttt tccgttttct gttacaaatt
    14761 cagcagttgc aaaatcatct ctccattttt ttaaaccttc aatatcaact tctgtgttgt
    14821 ccaccagatt tacatcaatg tgaatgagtg tagtattgta atttgatttt aatcctaccg
    14881 aaataatttt atttgtagca acttcgcgat gtaccataca agatgtacct tgtatcattc
    14941 cctgattgat taatttctgt gcaggctcat caatatttat atatcctaaa tcttttaatg
    15001 cctttgtcca gaaacgtaca tacaataaat gtcctgttgc atgttcgctg ccgccaatat
    15061 agagatcaac attctgccaa tagttcaaag catctttaga ggcaaaagaa gtgttgttgt
    15121 ttgcatccat ataacgtaag aagtaccaac tgctgccggc ccagccaggc atagttgtag
    15181 tttctaatgg aaacagattt ttgtatttcc agtttttagc tcttgccaat ggaggttcac
    15241 cggtttctgt cggtaaaaat ttatctactt caggtaggac taaaggcaac tcattttcag
    15301 gaattgcaac aggaatattt tctttataat aaattggaat aggctcgccc caataacgct
    15361 gccggccaaa cacagcatca cgcaggcggt agtttgtttt tcctttaccc agattttgtt
    15421 tttcaatttc tgttatacaa cagctaatgg cagatttcac atccaaatca tttaaaaaac
    15481 cggagttaat taattttcct tccttggcat cccatgaacc cttaccgaag tcatgtgtta
    15541 tattgggttc tatcacttgt ggtctatcga gtcccaaatg tgtagcaaaa cgaaagtcgc
    15601 gttcatcatg tgcaggcacg gccatcactg cgccagtgcc gtaaccggca agtacatagt
    15661 cgccaatcca aacaggaatt ttattgcctg taaaaggatg aatgacatag ctacctgtaa
    15721 acacaccact gattcgtgtt acttcagcct gacgttcgcg ctcacttctg ttttttgttt
    15781 tggtaacata atcctgcact tctgcacgtt gtgatgatgt tgttatttta tcaaccaact
    15841 catgttccgg tgctaatgtt acaaacgaaa caccaaataa tgtgtcggga cgtgtagtaa
    15901 atacttcaat atagtcatca gaattctgca aagaaaatct gatggttgtt ccttctgatt
    15961 ttcctatcca gttgcgctgt gcttctttca atgaatcgct ccaatcaata ttttctaatc
    16021 cctgcaacaa acgttcagca taggctgtta tgcgcatgct ccattgcttc atcagtttac
    16081 gctctaccgg atgaccacca cgttcactca atccgtcttt tacttcgtca ttggccaata
    16141 cggtgcctaa tgcaggacac cagtttacat acgcttcgcc taagtatgca atgcgatagg
    16201 ccatcagtat ccggcttttt tcagtttcat caaagttttt ccattgtgca gcagtgaaat
    16261 tttctatctc atcatgaaca gcactaatat cattattgcc gtttttttca aattctgaaa
    16321 tcagtttgtc aattgattcg gcacgattac ttttattgtt ataccatgca tcaaacaatt
    16381 ttaaaaaaat ccattgagtc catttgtagt aggaggggtc agaggtgcgc acttctctgt
    16441 cccaatcgaa tgaaaaaccc atcttatcca actgttcacg atagcgattg atgttggtct
    16501 cagtggtaat tgccgggtgc tgacctgtct gtattgcata ctgttctgca ggcaatccaa
    16561 aagaatcata tcccattgga tgcaaaacat taaaaccttt cagtctttta tagcgtgaat
    16621 aaatatcaga ggcaatataa cctaacggat ggccaacatg tagcccggca ccggaagggt
    16681 aggggaacat atccaacaca taaaactttg gcttgtcaga tttattttca accttaaaag
    16741 ttttgtttgc agcccaatat tgctgccatt ttttttctgt ttccctgaat tgatagtcca
    16801 ttatttgcgc tccgaaatga tgcgctaaaa tactgaaata ctgctgatgt ttgtattctt
    16861 tattgactta aaaagtcacg caccgttgta agcatacatt ccgggttgtc ggcatgaacc
    16921 caatggccgg cattggcaca ggtttttatt tctgcttcag ggaataagtt taatatttca
    16981 gacttatcat catcggttat atacttcgat ttctcacctt taataaataa ggttttcccc
    17041 tcgaattttt ctgttgatgt aattctttcg cctaccgatt caattttttc tgcaattact
    17101 ttcagattaa aacgccattg taaacgatcg ccttcccagt ataaattttt aagcaaaaac
    17161 atttttactc cggcatcatc aatatatcca gacagaatat gttcggcttc ttttcgggtg
    17221 ttaaccgtat cgagatctac tgcctgaagt gcttttatta tctgctgatg atgtggtgca
    17281 taataacgtg gtgcaatatc aacaactatc aaagatttta aaatatcctg atgcttcaaa
    17341 gcaaatttca ttgctacttt gccaccaaga gaatgcccca tgatgtttac attttgtaaa
    17401 tcgtgtttac tgataaaagc gaaaacatca tcagccattg aatcgtaatc aaacacagag
    17461 tcgtgtggcg aacttccatg attgcgcaag tcaacggcat acactgctat attattttcc
    17521 gttatttgtt ttgaaaaaga ttgccagtta tcattcagcc cgaataaacc atgcaaaata
    17581 attaacggct gaccattacc actgctgcga taaaataaat caagcgacat tattttctga
    17641 ttactgctcc gagatttttc tcaaaagctt tttcaatctt ctgcattact gattctattt
    17701 gtttatccgt taatgtagcc tcatcatcag ccaaagtaaa actcaatgca tacgattttt
    17761 ttcctgcttc aattttttcg ccttcataca catcaaagat gtcaacctgt ttcaataatt
    17821 tcttttcttg ctttaatgct aaatctctca ggtcggcata atttattgcc tgatcgagca
    17881 ttagtgaaag gtcgcgtttt acctccggaa atggttgtgg taatttgata gcagttttat
    17941 gcgataaact tttttccagc aaaacatcta tattcaatac tgcaagataa acgggctgtg
    18001 caatatcgaa ttgtttcaaa atatttttct ttactgatcc aatataccca aatgctgact
    18061 gcctttgcat gatgtttgcg aaagttgaaa acaaagtatg actatcctgc acccaattta
    18121 aatgagagct tatgcgaggc atcactaact caatagcttt ttctgcaaca gatttaagat
    18181 aaaataatga cgattctttt tctgacttat gccagttagg ttcattcacc ataccggtca
    18241 tagcaattac caaattgttt gtttctttaa gttttccttc atgctgaaga taggtcttac
    18301 caaattcaaa caattttaaa ttatgatttt ttctattgat attataacaa atagtgtgta
    18361 gggtagaagc aaccatatcg gggcgcaata tgtctaactc cttacttaac gggtttaaaa
    18421 ctttcacagc ctcatcaccg gcagatgaat gtgaggatga tactaaagaa ttattaatca
    18481 tttcaaaaaa cccgatagag ctcagatgat gtgaaatttt atattgcatt gcaggttttc
    18541 tgtcaggggt gtgacttgaa aaggatatac ccgttttttc attcaatgga atgtgatggt
    18601 aaccataagt ccgtaaaatt tcttcaacaa catcggcctg tcgggtaaca tcggttttat
    18661 atgtcggtat ggaaatggtt gcaccattta cattgctgct tttaatactg aagccacaat
    18721 actccaacac agaaagcaac atatcattac tgaaattaat tcctgatagc ttatttattt
    18781 tttcaaagtt tacatatata atgcgtggtt caacaacttc aggatacagg tcaacaacat
    18841 cggatgttat ttttccccct gcaacttctt ttataagtaa ggctgcacgt ttcaatgcat
    18901 atagtgttcc attaggatct gtgcctcttt caaaacgaaa tgacgattct gtctttaatc
    18961 catgatgttt ggcagcttta cgcacttcgg cagcattaaa ccaggcactc tctaaaaata
    19021 tttgtgttgt agaagcagac acaccggagt cggtgccacc catcacccct gcaatacaca
    19081 atggttctgt ggcattacat atcatcactt catcacctgt cagttttctt tcaatgtggt
    19141 cgagtgtagt aaatacagat gccttacctg ttttctgcac aataattttt cctttcactc
    19201 tggctgcatc aaaggcatgc aatggctgcc cggtttcaaa catcacaaag ttggttacat
    19261 caacaatgtt gttaatgcat ttaactccta tggactgcag tctgcttttc agccattccg
    19321 gtgattcgcc tactgttaca ccactaatag aaactccgca atatcttccg cagcctgaat
    19381 ttttatctac aactacgtca atgcgcagtg cgttgttttc agatttaaaa gcacccacat
    19441 caggcatact gaattgtgct gttgttgaat tattgttttc tgcttttgct cttgtattga
    19501 ttacagcagc aagatcgcgt gccacaccac catgcgaggc tgcatcaatg cggtttggtg
    19561 tcagacctat ttcaaaaata tgatcctgtt taattttaaa ccattcagca gcaggagtgc
    19621 ccggcacagc attggcatct aaaaccataa ttccctcatg cgaatttccg agtcctatct
    19681 catcctcaga acaaatcatc ccctctgagg cctgcccgcg tattttagaa ttctttatgt
    19741 cgaatggttc gccaaaggtt ggatacaatc gtgctccatt caaagcaacc aatacttttt
    19801 gtcctttggc aacattggcg gcaccgcaaa caatctgcaa gggaatagtt ttgcctacat
    19861 tcaccttcgt aacactcagt ctgtcggcat ctggatgttt ttccttctcc aacacttcgc
    19921 caacaaccaa gccacgcagt ccgcctttaa tgctgtcggt ttcgtccagc gactccactt
    19981 caagtccgct gcttgtcagt aaatgttcaa tttcttcagg tgtctgttgt aactttatat
    20041 agtccttcaa ccagtcgagc gatattttca ttcttatttt taatgaaagt gaaaatgcaa
    20101 aattaaatta ttcgggcaga caaaaatatt cagctattat ccctgctaaa ggtcgcttcc
    20161 aaaaccgctg ttatgctgat ggtctttttt gtcttgttca tacttgctgc agtctgtctc
    20221 tatactcagc ggatgttccg gtttttcaaa atcaccgttg tacaaattca ggtctttatc
    20281 ggcataagcc tttttcatat acaatgccca tataggcaat gctgtatttg caccctgacc
    20341 aaggctggta gttctgaaat gcactgagcg gtcttcgcaa cctacccaca cacctgtcac
    20401 caatgttggt gtaatgccca taaaccatcc atcactctga ttttgcgttg tacctgtttt
    20461 acctgcaatg ggatttgtca atccaaaacg aaaacgcaaa cgtccacccg ttccactttg
    20521 tacaacgccc tgcatcagat tcagcatcag ataagctgtc tcttcgctta tggcttcaac
    20581 cttgcgtggc acaaactctt gcagcaccat gccgttttta tcttcaattc gtgtaatgta
    20641 aattggctct gtccacacac ctttattggc aaatgtgctg tatgcaccac acatctcaaa
    20701 caaactcaca tcaggtgtgc ctaaacaaat tgaaggcact tcgtcaatgg gcgatgtaat
    20761 acccattttc cttgcaatct ctatcatcgg ttttggtcca aactgtttca tcagataggc
    20821 cgacacacta ttcaccgact cggcaagagc ttccttcaat gtcagcatgc cgccatattt
    20881 tccatcactg ttcgatgggc tccagtcggg ctgtccgggt ataggaatcg tcacacgcac
    20941 attagggact ttaaagcaag gcgaatagcc ttcctgcatg gcaagcgtat ataaaaacgg
    21001 tttaaatgtt gaacccacct gcctgcgtcc tgccttcacg tggtcgtatt taaagtagcg
    21061 ataattgttt ccgccaaccc atgcacgcac atagcctgtc tgtggctcaa tactcatcac
    21121 accggcttgc aaaaacatct tgtaatactt cagcgagtca agcggactca tcattgtatc
    21181 aatatcgccc ttccacgaaa aaatcgtcat cggtatttta gtggcaaaat tttcttttat
    21241 ctcctgctca ctcaaccctt cagctttaag cgaaatatag cggtcgcttt tcttaatacc
    21301 gtcctctata acttccggat gttgcgccca cggcactcga cccttccaat gctgataaaa
    21361 agttttttgc aactctgtca gatgttcctt catcccttct tcggcatact cctgcaatcg
    21421 cgaatctatc gtagtgtaaa tgcgcaaacc gtcacgatac aaattatagc tgctgccgtc
    21481 agatttttta tgctccttgc accacttcac cagttccaat cgcaaagtct ctcggaaata
    21541 aggtgccagt ccgtcattat gatcctctgc cctgaaattt aattttatcg gcagcttcac
    21601 tatagagtcg tatgctgcct cactcaaaaa tttatacttc tccatctgac tcaacaccgt
    21661 gttgcgtcta atcaatgcat tcttcgggtt acgcaccggg ctgtaacgtg taggtgcctg
    21721 cagcaaaccc accagcatgg ccgactcttc caccttcaac tgagatggca tcttattaaa
    21781 aaaagtcttt gctgccgatt ttattccata gctgttatga ccaaattcaa ccgtattcag
    21841 atacatggcc ataatctcct gcttggtata gttgcgctcc agtcgcacag caataatcca
    21901 ctccttaacc ttgcgcactg ctaaccgaaa accgctctca ttctcacgtg gaaacaagtt
    21961 cttggccaac tgttgtgtaa ttgtgctgcc accacccgaa gaacgctgct gcaaaatcac
    22021 cgtcttaaaa aacaccctcc ccaatccgcg catatccaca ccgctgtgct cctcaaaacg
    22081 aatatcttcc gtagccttta gtgcatcaac cgtattttcg gcaagctcct tataatgaat
    22141 gttcgaacgg ttctgaatat aatatttgcc caacaaaaca ttatcgctcg agattacctc
    22201 cgatgccaga ctgctgttcg gattctccaa ctcctcaaac gtaggcaact caccaaactt
    22261 acccatgttc
//
LOCUS       DAOOOL010000032        21311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_189_length_21311_cov_126.302879, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000032 DAOOOL010000000
VERSION     DAOOOL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_21311_cov_126.302879"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..7025
                     /locus_tag="PKU82_04310"
     CDS             24..7025
                     /locus_tag="PKU82_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82131.1"
                     /translation="MLTNGGTAPLQYSIDNGLNYQALNSFSNLPAGNYNVIVQDANGC
                     TVTTTVQLSQPSAIVLNPSATPSTCGNDDGTVTVTASGGTGAFNYSIDNGLTYQPSNI
                     FGQLYAGNYSVTVQDANGCTNTIAIAVNNLSAPSIQTLTPTNITCNGANNGTITVSAN
                     GGTGALSYSIDNGMSYQSGSVFQSLTPGNYNVVVQDGNGCTVTSTTTITEPLAIQINS
                     NATNTTCGNNNGSINIQGNGGTGILTYSNDGGTNFQVSGQFGNVLAGTYQIVVTDGNG
                     CTATSQVVVTDAPSPVIANMPLVQISCNGLSDGVITINTLGGLAPLQYSIDNGTTTQS
                     SGVFNNLSPGNYQIMVTDANGCTATSSATLSQPSGITANASTVNANCGTADGTITIAA
                     SGGSGTLQYSIDNGVSFQSASSFGFLVAGNYSIIIRDANGCTNTVTTTVSNNSAPAIS
                     AVSTTDITCFGAGNGSISVNANGGSGTLQYSINNGATFQASQIFNNVVAGTYTVIVQD
                     AGGCTVTDVVSVSEPLMLSINNTTSGSTCGLLNGQINISANGGTSPFQFSIDSGITFQ
                     STATFSGLASGNYQLQVQDANGCTINTTTLVANAPSPQISQVAHTDLTCFGSQNGTIS
                     IASSGGTSPVMYSVDGGLQFQAGAVFNNLNSGSYQIMIQDANGCTATSSVVVVEPSAI
                     TVTSVTTDATCGNSNGTLTINTIGGSGVLTFSIDSGLNFQSGSLFQNLLAGTYNLVVQ
                     DVNGCTSLASALVSNASAPVINATPATNVSCNGYNDGSISIQASGGLGTLTYSINGGI
                     TSFPSGIFNNLLAGSYNILVTDSTGCSVTAVVNITQPAAIISSTSVVNAICGSTNGEI
                     TITASGGVGTLQYSINNGGSFQSGNIFNSLGAGNYQIVVSDANGCTIGAVASVNNTNA
                     PVIQSTNVTNVSCFGGNNGSIIINANGGTGVLQYSINNVVTYSLNNTYTSLQPGNYSI
                     QVVDAAGCIATSNVVITEPDELLFNTTSTPSTCGNANGQITFNVIGGTAPLQYSVNNG
                     SSFQSGVSFTGLTATNYSVVIQDANGCSASAVATITNQAGPTIATVVTNDVKCNGAAN
                     GIISINANGGTGILQYSINNGSLFQQGNIFSGLSTGTYQIIIQDANGCSITAQSIINE
                     PDAILMSVVENGSTCSQPNGGINVSANGGTGSLQYSANNGISFQTTSSFLNLPSGNYT
                     VVVQDSNGCTAQMPATVTDAPAPVLQNINVTNVLCYGNSNGSVQVQVNGGTGAINYLL
                     SNGDVSSSGIFDSLTWGNYSVIITDANGCSVTGNAIVAQPQPLNFFVNTTASTCGNSN
                     GVITVNSITGGTGVCVFSLDSGLTNQSQNQFVNLNAGNYSVLATDSNGCSLQSNVLLA
                     NIAGPLFNALNITDALCSGAMNGSITVSPQGGTGPFLYSADSGFTYQAGNIFSALSAG
                     NYSLIIQDANGCLTDSIATIGEPLPLNYQVASAVATCGSSNGTITLNATGGNGSYQYS
                     INNGITFQSTGTFGGLIPAGYPVVVTDLNGCLDSGLVSVADAPSPVLTLVSVNDATCN
                     GFTDGVVSVTSQSGTLPIQYSINGGTWQQSGSFANLSAGNYSVIVQDSYGCTDTVTAL
                     IAEPLVIALQTSTIPVICNGQSNGQTAVTVTGGTMPYSYQWNAGATTASVNNLSAGTY
                     SVVITDANNCTSSATAVVTEPVVLQMTLATTPVTCFGYSNGIINTTASGGTGNLQFSI
                     DTGATYQSSTIFSSLTFGNYNIQVTDANGCTLSVPVSMTQPSAVALTSNTVDVKCFGG
                     NDGSLTANANGGTAPYQFVINNGNPQAGTTFNNLTAGNYVISVTDAMGCLKTISDSVG
                     QPTALSAVASGQNVLCNGQSNGAVNVIITGGVQPYMYQWTGGYTTSLVNNLPQGNYQV
                     TVTDFNGCTIHSSASITQPTAINLGLTPDPTICIGQSASVTANANGGTPPYVFKWNTG
                     HVGATLQASPVITTTYNVFLTDSNGCNSPLRYANINVNPALALVMTDDDTLCEGQSTT
                     LTATPSGGNGGPYIINWNGQTQPATSITVAPTQTTNYVATLTDGCTLMPATANAVVVV
                     NPLPQVVFVPLITEGCVPLIVDFQNMSSNGASWQWTFGDGDKDTLINPTHTYTQPGTY
                     SVGLYAVSNAGCENQLVLDDAIQVYENPQAAFTSNRVEASILDPWFEFRNQSIGASTY
                     LWDFGDQRTQSTEINPMYVYGDTGTYQIIMTATSEHQCSDTAYGEVHVSGAVTVYIPN
                     AFTPNSDGRNELFSVSGIGYTELQMIIFDRWGNMLFNQTSSNPGWNGMNMFNNSMCQQ
                     GVYVYKVIVKNIFNKQQEFKGTVSLLR"
     gene            complement(7266..8114)
                     /locus_tag="PKU82_04315"
     CDS             complement(7266..8114)
                     /locus_tag="PKU82_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ82132.1"
                     /translation="MKKGFLFFVLGGMMLTSCVSQKKYKTQVSKYDTLKTDYDKVNYQ
                     LSACLDEQQKKSAKMSEMEKELTYLKANSTTMLKQLSDLSVLTTTQSESIRKSLENIN
                     AKDIFIQDLQREMSRKDSLNMALVMNLKGALKDVNDKDVEIKVEGSAVFISISDKMLF
                     RSGSYEITPKAKEVLGKVAEVIKAQPEVQFMVEGHTDNKSIHTAFIKDNWDLSVLRAT
                     SVVRVLQKNYGVDPSRIIAAGRSEYVSLQPNTTAEGRAMNRRTRIVILPQLDQFFKLM
                     EPKTKP"
     gene            8318..11158
                     /locus_tag="PKU82_04320"
     CDS             8318..11158
                     /locus_tag="PKU82_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82133.1"
                     /translation="MPIEKIPYFRKLKPIYTNIMNRILRLTMFFALIFGAEKATACSV
                     AGTVTSNRDSVCYQDTITLTTTGYTGTVFQWQSDNGGGWVNEAGAGATTDTYTFVPGE
                     TRQYRIIVTATGCPADTSNILTVTVGSIPVPTGTGASRCGYGQLTLTGTGSANGSLRW
                     FTAPSGGIPIGTGNSVNVTVGATTTFYLEDNTSGGGGGASPLIITEFDINDEASGSSG
                     DDLEIQNVSSLPLDVTGWRVAINNSYSDINLVNANIQTLSGIIPPGGILSWGDATTAT
                     SYWGSNILWNSGAFPTFTGWAMILDNNNVVRDFVPLNWPAANIAAMNTNVGGTPVTIP
                     PTLWTGDGVNITTVGAGMGVARTGNIDNNSNADFVIQTLDIGTTNAGMIIPFQGFGCS
                     SPRVPVVATINASTPVTINATSTALCMGQSSTLTVSSSNSNYSYTWTPAAGLNTTSGA
                     TVTATPTVPTTYYAIAVDGSCGAIDSVFIDVGPTSIAGTASVTSSTLCAGTNTTLNLN
                     GSLGSIQWQSNDGSGWVNMSGATTAPYQFAPTVTADYQAIVTSGGCPSATSNMLSVTV
                     IPVTTPTTVNDTICAGGTANLQSSGTGIVNWFTSQTGDTLVNTGNTYNVSLAATTTFY
                     VQTVDGGNVYHVGPADRSIGSQASNAPNNYGMRFDVLQEVTIDSVYIYPVTAGTVTIN
                     LVDATGANILNTKTITVPNTFNIYPVYLGFSVTGGTSYRLIVASGSVNLYYNTSGAIY
                     PYAVAGSPLSITGYYNPNPNTGAAYYYLYNWVITSGCKSGYIPVTGVVQGPPMPTITP
                     SGSLLTSSTAANYQWLLNGNIIPGANSQTYQTTQPGSYTVQVTDATTGCIVTSLPFMI
                     VSISDPNAIAAGVSIYPNPSTDKFYLNFNEAVLGNASVKLFNGVGQLIYVTEVNNVTG
                     KTVELEKDLSKGIYFMQLTTEKGTFQTKIIRE"
     gene            complement(11197..11505)
                     /locus_tag="PKU82_04325"
     CDS             complement(11197..11505)
                     /locus_tag="PKU82_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOZ82134.1"
                     /translation="MDNKNENQLNIELSEEIADGIYSNLAIITHSNSEFVVDFIKVMP
                     GVPKAKVKSRIVLTPQHAKRLMTALAENVAKYEQAHGTIKQTDGIAIPMNFGGPTAQA
                     "
     gene            11658..11731
                     /locus_tag="PKU82_04330"
     tRNA            11658..11731
                     /locus_tag="PKU82_04330"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11692..11694,aa:Asp,seq:gtc)
     gene            12070..12993
                     /locus_tag="PKU82_04335"
     CDS             12070..12993
                     /locus_tag="PKU82_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ82135.1"
                     /translation="MIDVINLKKSYGEREAVKSLNFLIKDGEFYGLLGPNGAGKTTTI
                     SIMSTILDADAGDVVIDNYNIKKNPKKVKQVIGVVPQEIALYNELSAGENLKFWGGLY
                     NVPESELKARMDELLKWFGLYDRRNDKVGSYSGGMKRRINIAAALLHRPKVLFMDEPT
                     VGIDPQSRNCIYEVIEKLHRDGMTIVYTTHYMEEAERFCDRIGIIDEGRIIAEGTLDD
                     LKKSKAIKEVISITFSKLSDTLVNELTSFWRDLKHQDDKILFYSNDIKTDLPKLVSKC
                     NQLSADIANIEIQKTNLETIFLSLTGKQLRD"
     gene            12995..14266
                     /locus_tag="PKU82_04340"
     CDS             12995..14266
                     /locus_tag="PKU82_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ82136.1"
                     /translation="MIKLAIKDLMLFIKDKRSMVLTFAIPIALITLFSFAFGGAGKNK
                     EDQKITLLLSDLDKTKLSEAAIEKLDSLKSIQIVQIQLDSAQNAIKKGNESCVLIIHK
                     GFADSLLAGNVLPLELQYDEARDIEVGLLQQSLIPTVAMLPFDLGNTQQVMANKFQKM
                     VGNSSQNSKANIQMKSDSLFSAIYQGMANNNTGGGANASDAFFDSGIKMTKLIASKND
                     NALGLIHAVAGTSVMMLLFSVVGIGVGLLDEKQEGTLKRLLYSPIDPIHILFGKLITA
                     NIISIFQLVVMFAFSGLVFGLDVSAHLPGLIIAIVATAFACSAFGVFLASFAKSRQQV
                     QGLSTLIILVMSAIGGSMMPLFFMPAFMQKLAVVSVNYWSIQSFYDIFWRNLSITSVT
                     FLSRIGVLLLIGTILNIIAVYLFRKNILKLT"
     gene            complement(14299..15402)
                     /locus_tag="PKU82_04345"
     CDS             complement(14299..15402)
                     /locus_tag="PKU82_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOZ82137.1"
                     /translation="MYLSNLSLVNFKNYTEAQLNFSSGVNCFTGSNGSGKTNIIDAVY
                     YLSFTKSYFNISDTQNIHHGESLFVIQGKFVVNEKEEDVFCGVKTGFKKQVKRGNEEY
                     ERLSDHIGLFPIVMVAPVDHILIAEGSDERRKFIDSIISQVNKPYLENLISYNKALMH
                     RNSYLKQLHGRMPDPSMMEVWDEQLVNYGSAIEKERRKFIAEFIPLFNETYNYLADHA
                     EEVSINYQTQLEQDDFRTLLKLSLQKDIALQHTSAGIHKDELVFKLGKYPLKRIASQG
                     QQKTFLMAVKIAQFEYLFRHKKTKPILLLDDIFDKLDDFRVKRLMELVSRHTFGQIFI
                     TDTHPERLKKIFEDISVNIKLFEVSQGMINEVS"
     gene            complement(15430..16554)
                     /locus_tag="PKU82_04350"
     CDS             complement(15430..16554)
                     /locus_tag="PKU82_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOZ82138.1"
                     /translation="MKYIVTLCRILVGVLFIISGFIKANDTLGFSYKLDEYFLVFNMS
                     FMSAFSVALAQFICVAEIVLGVATLVGWRMNLVTWLLMLMIVFFTFLTFYSAYFNVVK
                     DCGCFGDALKLTPWQSFGKDVVLLVLILVIFAWRNKVKPLFELKKTRWVVIFALFASS
                     LFTYYTYAHLPVIDFRPYAIGNNLKIGMLPPPNARPDSVVIVFKYKAKDGTIKEFGMN
                     NLPEDLENYEFVDRTDKVIREGDKPKIHDFTIVDADGHDHTQEFLNNPQYSFMLVAYD
                     LNKSNTKIQSKVNQLADACSKNKVQFFGLTSTVAAETDLFRHENQNMFDYYFCDGTTL
                     KTIIRSNPGLVLLQNGVVVAMWHYNDFPSWDDVNKQYLKK"
     gene            16686..17528
                     /gene="folP"
                     /locus_tag="PKU82_04355"
     CDS             16686..17528
                     /gene="folP"
                     /locus_tag="PKU82_04355"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOZ82139.1"
                     /translation="MQTFTSINCCGKLINLHKPHVMGVLNLTPDSFYDGGKYNSENAI
                     LTQVALMLNEGATFIDIGGSSSRPGSKMPTEEEEWSRIGNVIALIKQHFGDVLISVDT
                     VYSVIAKRAVDAGACMINDISAGLMDSKMIETVAALKVPYLMMHMKGRPENMQDNPVY
                     DNVLKEVIDFFSKQLLLCRKVGINDILIDPGFGFGKTVEHNFELLAKLNLLKIVGLPI
                     VAGLSRKGMIWKTLDITPSEALNGTTALNMVALMNGARILRVHDVKEAMQCIKLFNKL
                     NEVR"
     gene            complement(17544..18203)
                     /locus_tag="PKU82_04360"
     CDS             complement(17544..18203)
                     /locus_tag="PKU82_04360"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOZ82140.1"
                     /translation="MTIHREGKNWVWGTLITVLLINFLVLNFRAEHDWITVLVLIVTI
                     VFFLLILQFFRYPKRNITRNENYVIAPADGKVVVIERTEESEYYKDKRIQVSIFMSPI
                     NVHANWYPMSGKIKFLRYHKGKYLVAWHPKASTENERSTIVVEKDSNKTILLRQIAGA
                     LAKRIVYYPRENDLVKQGAEMGFIKFGSRVDIYLPLTAKINVELNQKTIGGVTVIAEL
                     A"
     gene            complement(18234..19511)
                     /locus_tag="PKU82_04365"
     CDS             complement(18234..19511)
                     /locus_tag="PKU82_04365"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOZ82141.1"
                     /translation="MADSKKAKQNHEEENPFEEMIKRLDVAAKIMKLDSEVYEIMKKP
                     SKLVYCSMPVKMDNDKTKVFEGFRVVHSTALGPSKGGVRYSTYVNENEVMALAAWMTF
                     KCAVADIPYGGAKGGITCDPSTMSKGELERLTRAYTNAMVDVFGVDKDIPAPDMNTGP
                     QEMAWIVDEYSKLKGGFTPGVVTGKPIHLGGSLGRSEATGRGVMTATMEAMAKMGLNP
                     AKCRAAVQGFGNVGSITAKHYEAKGLKIVAISDHTAAFYNPDGIDIEKAIKYRNSNKG
                     VIKGFKGGKLISNEELLTLNVDVLAPCAMENQITDENAGKIKAKLIVEGANGPTTDEA
                     DHILSKKGTVVIPDILANGGGVTVSYFEWGQNRSGLYMTEEEVNTKADHWMKQAFHNV
                     WNTSVKHKTTMRIAAYIYALGKIELGIKSRGHY"
     gene            complement(19743..20762)
                     /gene="murB"
                     /locus_tag="PKU82_04370"
     CDS             complement(19743..20762)
                     /gene="murB"
                     /locus_tag="PKU82_04370"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOZ82142.1"
                     /translation="MPMRENVSLKNLNSFGIEANARYYIDIHSEDDLILFLLQHKKTT
                     LPLLVMGGGSNMLFFKDFDGIVLKNNLHGIDVVEENEASVKIRIGAGEVWHNVVMWSV
                     EKNLGGIENLSLIPGTAGAAPIQNIGAYGVEIKNSLVAVEALAIDSAEKKVFNNHECE
                     FGYRNSVFKNAAKGKYIITAIILELKKQPQFKTEYGAIHSELEKQGIKNLSVKAISDA
                     VIAIRRSKLPDPAVIGNAGSFFKNPEITEQEFEKIKQQHPNIPHYPGAANKIKLAAGW
                     LIEQCGWKGFREGDAGCHKNQALVLVNYGNATGAEIYSLALKIKESVYNHFGVHIEPE
                     VNIIQ"
     gene            complement(20762..>21311)
                     /locus_tag="PKU82_04375"
     CDS             complement(20762..>21311)
                     /locus_tag="PKU82_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82143.1"
                     /translation="TAVDSFTFVEPDPIVINFTVIDASCPGCTDGSIIATATGGTPPY
                     DYLYPALGIADSAATNLGMGYYLFCAQDFNNCMQCDSVFVDEATAINSLSPEIKEIKI
                     FPNPFADKAYLSITTAESKDFKLYFFDVAGRIVNIPYNHAGNTENKQVFIIQNNGLKA
                     GLYYVKIMSANEIIAIGKFIVN"
BASE COUNT         6590 a         3852 c         4040 g         6829 t
ORIGIN      
        1 gttcaagtga tggtgcagtt cagatgctga ctaatggcgg cacagcacct ttgcaatatt
       61 ctattgacaa tggtttaaat tatcaggcat taaatagttt cagcaattta cctgcaggaa
      121 actataacgt tattgtacaa gatgcaaatg gctgtactgt gacaacgact gttcaactga
      181 gtcaaccttc tgcaattgtt ttaaatccat ctgcaacacc aagtacttgt ggtaatgatg
      241 acggtactgt tacagtaaca gcaagtggtg gaacaggagc ctttaattat agtattgaca
      301 atggacttac ctatcaacca tcaaatattt ttggtcagtt atatgccgga aattattctg
      361 taacagtaca agatgccaat ggttgtacca acacaattgc aattgcagtc aataaccttt
      421 ccgcaccttc tattcaaact ttaacaccaa caaatattac ctgtaatgga gctaataacg
      481 gtactatcac agtatctgcg aatggtggaa caggtgcttt gagctactca atagataatg
      541 gaatgagtta tcagtccggt tcagtttttc agtcattaac tcctggaaat tacaatgttg
      601 ttgttcagga tggcaacggt tgtacagtta catcaacgac aacaattacc gaaccattag
      661 ccattcaaat aaatagtaat gcaactaata caacctgtgg aaacaataat ggctctataa
      721 atattcaggg aaatggtggt acaggaattt taacttattc aaatgatggt ggaacaaact
      781 ttcaggtatc aggacaattt ggtaatgttt tagcaggaac atatcagatt gtagttacag
      841 atggcaatgg ttgtacggca acatcacaag ttgttgttac cgatgcacca tcacctgtaa
      901 ttgccaacat gcctttagtg cagatttcct gtaatggact aagtgatgga gtaattacta
      961 ttaatacact tggaggatta gccccattgc aatattcaat tgacaatgga actactacac
     1021 aatcatcagg tgtgtttaat aatctatcgc ccggcaatta tcagataatg gttactgatg
     1081 ctaatggttg tacagcaact tcgtctgcta cattaagtca accttcagga attacagcaa
     1141 acgcttcaac agttaatgcc aattgtggca cagctgatgg tacaataaca attgcagcta
     1201 gcggtggcag tggaacattg caatattcaa ttgataatgg cgtttcgttt cagagtgcat
     1261 cttcctttgg atttcttgtt gcgggaaatt atagcataat aattcgtgat gcaaacggat
     1321 gtaccaatac cgtgacaacc acagtaagta ataattctgc tccagcaatt tctgcagtga
     1381 gtactactga tataacatgt tttggtgcag gcaatggcag catttcagta aatgcaaacg
     1441 gtggttccgg cacattgcaa tattcgataa ataatggtgc aacatttcag gcttctcaaa
     1501 tattcaacaa cgtagttgcc ggaacttaca ctgtgatagt tcaggatgca ggtggttgta
     1561 cagtaacaga tgtagtctct gtttcagaac cattaatgtt gagtattaat aatacaacaa
     1621 gtggttcgac atgcggatta ttgaatggtc agataaatat ttcagcaaat ggcggcacat
     1681 caccatttca gttttctatt gatagtggaa taacatttca aagtacagca acattctccg
     1741 gattagcttc aggaaattac caactgcagg ttcaggatgc aaacggttgt acaattaata
     1801 caacaactct tgtagctaat gcaccatcac cacaaatcag tcaggttgca catacagact
     1861 taacttgttt tggaagtcag aatggtacaa tatcaattgc ttcctccggt ggaacttcac
     1921 ctgtcatgta ttcagttgat ggtggtttac agtttcaagc cggtgctgta tttaataatt
     1981 tgaattcagg cagctatcag ataatgatac aagatgcgaa tggatgcaca gctacatctt
     2041 cagtagttgt tgttgagcca tctgcaataa ctgtaacctc agttactact gatgccactt
     2101 gcggaaacag taatggtacc ttaacaatta ataccattgg aggttccgga gtacttacct
     2161 tcagtattga tagcggattg aattttcaga gtggaagttt gtttcagaac ttattagcag
     2221 gaacatataa tcttgttgta caggatgtca atggctgtac atctttagca agtgcattag
     2281 ttagcaatgc atctgcaccg gtaattaatg caacacctgc aacaaatgtt tcatgcaacg
     2341 gttataatga tggtagcatt tcaattcagg caagtggtgg tttgggaaca ttaacatata
     2401 gtattaatgg tggtattact tcatttccaa gtggcatatt taataatctg cttgcaggaa
     2461 gttataatat tttagttaca gattctacag gttgtagtgt aactgctgtt gtaaatatca
     2521 ctcagccggc agctataatt tcaagtacat ctgttgtaaa tgctatttgt ggatcaacaa
     2581 atggcgaaat aaccattaca gcaagtggcg gtgtgggtac cttgcaatat tcaattaata
     2641 atggcggaag tttccaatca ggaaatattt ttaatagcct cggtgcgggg aattatcaga
     2701 tagtagtgag tgatgcaaac ggttgtacca ttggcgcagt ggcctcagtt aataatacca
     2761 atgcacccgt aatacaatca accaatgtta ctaatgttag ttgctttgga ggaaacaatg
     2821 gttctatcat tattaatgct aatggtggaa caggtgtact gcagtattca ataaataatg
     2881 ttgttacata ttcgctaaac aatacttata cgagtttaca accgggtaac tattccattc
     2941 aggtggttga tgctgccgga tgtattgcta cttcaaatgt tgtaattaca gaacctgatg
     3001 aattgttgtt taatacaact tccactcctt ctacttgcgg aaatgccaac ggacaaatta
     3061 cattcaatgt cattggagga acagcaccac ttcaatattc tgttaataat ggttcaagtt
     3121 ttcaatcagg tgtaagtttt accggtttga cagcaacaaa ttattccgtt gtgatacaag
     3181 atgccaatgg atgttccgca agtgcagttg caacaataac caatcaggca ggtccaacaa
     3241 ttgctaccgt tgtaaccaat gatgtaaaat gtaatggtgc agcaaatggt attatttcta
     3301 taaatgctaa tggaggcaca ggaattctgc aatattctat caacaatggt tcattatttc
     3361 aacaaggaaa tattttttcc ggattgtcaa ctggtactta tcagataatt attcaggatg
     3421 ccaatggatg cagcataact gcacaatcta taataaatga acctgatgct attttaatgt
     3481 ctgttgtgga aaacggttca acttgcagcc aacccaatgg tggcatcaat gtttctgcaa
     3541 atggaggcac aggatcgtta cagtattctg ctaacaatgg tatatcattt cagacaactt
     3601 cttctttctt aaatctgcca tcaggaaatt atacagtagt agttcaggat agcaatggat
     3661 gcactgcgca aatgccggca accgttactg atgcacctgc acctgtgctg cagaatatta
     3721 atgtgacaaa tgttttatgt tatggcaata gtaatggatc agttcaggtt caggtaaatg
     3781 gtggcacagg agcaataaac tatttattaa gcaatggtga tgttagttcg tcaggaattt
     3841 tcgattcatt aacgtggggt aattattcag taattattac cgatgcaaac ggttgtagtg
     3901 ttacaggaaa tgcaattgtt gcacaaccac agcctttgaa tttctttgtt aacacaacgg
     3961 catcaacctg tggtaatagc aatggtgtaa tcacagtaaa tagtataaca ggaggaacag
     4021 gagtttgtgt tttctcttta gatagcggat tgaccaatca gtcacaaaat caatttgtga
     4081 atttgaatgc cggaaattat tctgtgctag ctacagattc aaatggttgt tcattgcaat
     4141 cgaatgtttt attggcaaat attgcaggtc ctttatttaa tgcattgaat attacagatg
     4201 cactgtgttc cggtgccatg aatggctcaa taaccgttag cccacaaggt ggcacaggac
     4261 cgtttttgta tagtgcagat agtggtttta cttatcaggc aggtaatatt tttagcgcat
     4321 tatcagcagg aaattatagt ttaattattc aggatgccaa cggatgttta acagacagta
     4381 ttgcaacaat aggtgaacct ttgcctctga actatcaggt tgcttcggca gtggctacat
     4441 gtggtagcag taatggaaca atcacattaa atgccacagg tggtaatgga agttatcagt
     4501 atagtatcaa taatggaata acatttcagt caacaggaac ttttggaggt ttgattcctg
     4561 caggttatcc tgtagtggta acagatttga atggatgttt ggacagtgga ttggtaagtg
     4621 ttgcagatgc gccatcacca gtattaactt tagtatctgt taatgatgca acctgtaacg
     4681 gatttacaga cggtgttgtt tctgttacat cacagagtgg tacattgcca attcagtaca
     4741 gtataaacgg aggaacttgg cagcagtcag gtagctttgc taatttatct gcaggaaatt
     4801 attcagtaat agttcaggat agttatggct gtacagacac agttactgcg ttaatagcag
     4861 aaccgttagt aattgcattg cagacttcaa caatacctgt tatttgtaat ggacaaagta
     4921 acggacagac tgcagtgaca gttaccggtg gtacaatgcc atattcttat caatggaatg
     4981 cgggagcgac cacagcttct gtgaataatc tttctgccgg aacatattcc gttgttatta
     5041 ctgatgccaa caattgtaca tcatcagcaa ctgctgtagt aacagagcct gtggtattac
     5101 aaatgacatt ggctacaaca ccggtaacat gttttggcta tagcaatggt atcattaata
     5161 caacagcatc aggggggaca ggtaatctgc aattctctat agatacagga gcaacctatc
     5221 agagttcaac tatattttca tcattgactt ttggaaatta taatatacaa gttaccgatg
     5281 ccaatggctg tacattatct gttccggtta gtatgacaca gccttctgct gttgccctga
     5341 cttcaaatac agttgatgtg aaatgttttg gtggcaatga tggaagttta actgcaaatg
     5401 caaatggagg aactgcacct tatcagtttg ttattaataa cggtaatccg caagcaggta
     5461 caacatttaa taatctgaca gcaggtaatt atgttatttc agttacagac gctatgggtt
     5521 gtttgaaaac aatttcagac tctgtagggc agccaacggc tttaagtgct gttgcttccg
     5581 ggcagaatgt tttatgtaac ggtcagagca atggcgcagt taatgttatc attaccggag
     5641 gtgttcaacc ttatatgtat caatggacag gaggttacac cacatcactt gtaaacaatt
     5701 tgcctcaggg aaattatcag gtaacggtta cagattttaa tggatgcact attcattcat
     5761 cggcaagtat aacacaacca actgcgataa atttaggatt aacccctgac ccgactattt
     5821 gcattggaca atctgcatcg gtgacagcaa atgctaatgg tggtacgcct ccttatgtgt
     5881 ttaaatggaa tacaggacat gttggtgcaa ctttgcaggc ttcacccgta attacaacaa
     5941 catataatgt gtttttaact gatagcaatg gttgtaattc gcctttgcgt tatgcaaata
     6001 ttaatgtcaa tcctgcctta gctttggtaa tgactgacga tgacacctta tgcgaaggac
     6061 aaagcactac cttaactgca acaccttccg gtggcaatgg cggaccctat atcatcaact
     6121 ggaatgggca aacacaacct gcaacatcaa ttacagttgc accaactcaa accaccaatt
     6181 atgttgctac tctgactgat ggatgtactt taatgccggc tacagcaaat gcagtagttg
     6241 ttgttaatcc gttgccacaa gtagtttttg ttccattgat aacagagggt tgcgtgccgc
     6301 tgattgttga ttttcagaat atgtcttcta atggtgcttc atggcaatgg acttttggcg
     6361 atggtgataa agacacttta ataaatccta cgcacaccta tacacagccc ggaacttact
     6421 cagtgggcct ttacgcagtt agtaatgcag gatgcgaaaa tcaacttgtt cttgatgatg
     6481 ccattcaggt ttatgaaaac ccgcaagctg catttacaag caacagggta gaagcatcaa
     6541 ttcttgaccc atggtttgag ttcagaaatc aaagtattgg ggcttccaca tatctgtggg
     6601 attttggtga tcagcgaaca caatcaacag aaatcaatcc tatgtatgtt tatggtgaca
     6661 ccggaaccta tcagatcata atgactgcca cctctgaaca tcagtgtagc gatactgcct
     6721 atggcgaagt gcatgttagt ggtgctgtta ctgtgtatat tccaaatgca tttacgccaa
     6781 atagtgacgg gcgtaatgaa ttgttttctg tttcaggtat tggctataca gaattgcaga
     6841 tgataatttt tgaccgatgg ggaaatatgt tgtttaatca gacttcttca aatccgggat
     6901 ggaatggcat gaatatgttt aataattcaa tgtgtcagca aggagtttat gtttataaag
     6961 tgattgtaaa aaacatattc aacaaacaac aggagtttaa aggaactgta agtttattga
     7021 ggtaaattgt aagtgaaaaa aaagctgctc tattgagctg tcttttttgt tattgtctgt
     7081 attgtacaat gtctttcatt attacaaatc atgcattttt gttttgacct gacccttagg
     7141 tttgtgcaat cataaactct cccgcatttc aggggacgtt gaatggacaa aaggtaaaaa
     7201 tatatgataa aaatttagct cttcacaaat ttttgcgaag ggcttttgct ttaatttaag
     7261 tattgttatg gcttagtttt tggctccatc aatttaaaga actgatcgag ttgtggcaga
     7321 ataacaattc tggttctgcg gttcatggca cgtccttctg cagttgtgtt aggctgaaga
     7381 cttacatact cactgcgtcc ggctgcaatg atgcgtgatg gatcaacacc ataatttttt
     7441 tgtaaaacac gaactacaga cgtagcacgc agtacactta aatcccaatt gtcttttata
     7501 aatgctgtgt gaatagattt attgtcagta tgaccttcaa ccataaactg cacttcaggt
     7561 tgggctttga ttacttctgc tacttttcct aaaacttctt ttgcctttgg tgtaatttca
     7621 taacttccac ttctgaaaag cattttatcg gaaatagaaa taaacacagc actgccttca
     7681 acttttatct ctacatcttt atcgttaaca tctttcaatg cgcctttaag gttcattact
     7741 aatgccatat tcaaagaatc ttttcttgac atttctcgct gcaagtcctg aataaaaata
     7801 tcttttgcat taatattttc gagcgatttg cggatacttt cactttgtgt ggttgtcaat
     7861 acagaaaggt ctgatagctg tttcagcatt gtagtactgt tggctttaag atacgtcagt
     7921 tccttttcca tttccgacat ttttgcagat tttttttgtt gctcatccaa acaggcacta
     7981 agctgataat ttaccttatc ataatcagtt ttcaaagtgt cgtatttact cacctgtgtt
     8041 ttgtattttt tctgactcac gcatgaagtc aacatcattc cccccaaaac aaaaaataaa
     8101 aatccttttt tcattatttt taaaattaaa agttaaacat aaaattgaga atgcaaaggt
     8161 atccaaccat atcgctataa atgtttaagc aagcattaat gtttccacaa aaggtatgtt
     8221 aagtaagatt atcattattt gttcacatat aaaaccatca ataaatgatt tctaaaaaag
     8281 ttctgcagta aaaatcagat tagataattt tctactgatg ccgatagaaa aaattcctta
     8341 ttttcgtaaa ctgaaaccaa tttataccaa tatcatgaat agaattttac gattaacgat
     8401 gttttttgcc ctgatttttg gagcagaaaa ggcaacagca tgttctgttg caggaacggt
     8461 aacctcaaac cgcgattcag tatgttatca ggacacaatt acgctaacta ccacaggcta
     8521 taccggaacg gtttttcaat ggcagagtga taacggtggt ggttgggtaa atgaagcagg
     8581 tgcaggtgcc acaacagaca cctatacttt tgtgcctggt gaaaccagac aatacagaat
     8641 tattgttaca gcaaccggtt gccctgcaga cacttcaaat attttaacag taacagtggg
     8701 ttcaattcct gttccaacag gaactggcgc atcacgttgc ggttatgggc aacttacttt
     8761 aacaggaaca ggatctgcaa atggttcttt acgatggttt actgcaccaa gcggtggtat
     8821 tcctatcggc acaggaaatt ctgtgaacgt tacagtaggt gccacaacta cattctactt
     8881 agaggacaac acttccggtg gaggtggtgg tgcaagtcca ctgataataa cagagtttga
     8941 tattaatgat gaggcttcag gaagtagtgg tgatgacctt gagattcaaa atgtatcgtc
     9001 cctgccactt gatgttaccg gctggcgtgt agcaattaat aattcttatt cagacataaa
     9061 tctggtaaat gctaatattc aaacattgag tggaataatt cctccgggtg gcattttaag
     9121 ttggggtgat gctacaactg caacaagcta ctggggctct aatattcttt ggaactccgg
     9181 ggcatttccg acatttacag gctgggcaat gattcttgat aacaacaatg ttgtcagaga
     9241 ttttgttcca ttaaactggc ctgcagccaa catagctgca atgaatacaa acgttggcgg
     9301 aactcccgtt accattccac ccacattgtg gacaggtgat ggtgtaaata tcactacagt
     9361 tggtgccgga atgggcgttg cacgtaccgg taatattgat aacaattcca atgcagactt
     9421 tgtgattcaa acattagata taggaacaac aaatgcaggt atgataattc cattccaggg
     9481 cttcggctgt tcaagcccaa gagttcctgt tgtagcaaca atcaatgctt ctacacctgt
     9541 aaccatcaat gcaacttcaa cagcattatg tatgggacaa tcttcaacat taacggtgag
     9601 cagcagcaac agcaattata gttatacatg gacacctgct gcaggattaa acacaacttc
     9661 aggcgcaaca gtaactgcaa caccaacagt tcctactaca tattatgcaa tagccgttga
     9721 tggaagttgc ggagcaatag acagtgtgtt tattgatgta ggaccaacat ctattgcagg
     9781 aacagcttct gttacatcat caaccttatg tgcagggact aacacaacat taaatcttaa
     9841 cggttcacta ggaagtattc aatggcaatc aaatgatggt agtggttggg taaatatgag
     9901 tggtgctaca acagcacctt atcagtttgc acctacagtt actgccgatt atcaggccat
     9961 agttaccagc ggtggctgcc cttcggcaac ttctaatatg ctttctgtta ctgtgattcc
    10021 tgtaacaaca ccaactactg tgaacgatac aatttgtgcc ggtggaacag ctaacctgca
    10081 atcaagcggt acaggcattg taaactggtt tacttcgcaa acaggtgata cgttggttaa
    10141 tacaggtaat acatataatg tttcattagc cgcaacaact acattttatg tgcagactgt
    10201 tgacggtgga aatgtatatc atgtaggtcc tgccgatcgt agtatcggta gccaggcaag
    10261 caatgctcca aataactatg gaatgcgttt tgatgttttg caggaagtaa caattgactc
    10321 agtttatatt tacccggtta cagcaggaac agttaccatt aatcttgtag atgctaccgg
    10381 agcaaatatt ctaaacacca aaactattac agttcctaat acttttaata tttatccggt
    10441 atatcttgga ttcagtgtta ccggtggaac atcatataga ttgatagttg cctcaggaag
    10501 cgtaaatctt tattacaaca catctggtgc aatttatcct tatgcagttg caggtagccc
    10561 attatcaatt acaggatatt ataaccctaa tccaaatacc ggtgcagcat attattatct
    10621 ctacaattgg gttatcacca gtggatgtaa gagtggttat atccctgtaa caggagtggt
    10681 gcagggtccg ccaatgccaa caattactcc aagtggttca cttttaactt catcaacagc
    10741 agcaaattat cagtggttat tgaatggaaa tattattcca ggagcaaaca gtcagactta
    10801 tcagacaaca caaccgggtt cttatactgt tcaggtgact gatgctacaa caggatgcat
    10861 agtaacttca ttacctttta tgattgtttc tataagcgat ccaaatgcaa ttgctgcagg
    10921 agtaagcatt tatccaaacc catcaacaga taaattttac ttgaatttta atgaagctgt
    10981 tttaggaaat gcttcggtga aattatttaa tggagtagga cagttgattt atgtaactga
    11041 agtaaataat gtaacgggta aaacagtaga attagagaaa gatttatcaa aaggaatcta
    11101 ctttatgcaa ctgaccacag aaaaagggac atttcagaca aaaattattc gcgaataaaa
    11161 accaaaaaag aaaccgccct tgaggcggtt tcttttttaa gcttgtgctg ttggcccacc
    11221 gaaattcatc ggtatggcta tgccatcagt ttgtttaatt gtgccatgtg cctgctcata
    11281 ttttgcaaca ttttcggcta aagctgtcat cagcctttta gcatgttgag gcgttaaaac
    11341 aattcttgat ttaacttttg ccttaggaac accaggcata actttaataa aatcaaccac
    11401 aaactcggag tttgaatggg ttatgatggc cagattggaa taaataccat ctgcaatttc
    11461 ctcactcagt tcaatattta actgattctc gtttttattg tccattgtct gaattttctt
    11521 caaagatacg gttaattcac aagccgtctt atttaaaatt cacaggcgaa gggctgactt
    11581 gaacagaaat agcaataaaa aagtaaagat tatttgtaga aagaataaaa aatctatctt
    11641 tgcgccctct aaaagagggt ccggtagttc agttggttag aatacatgcc tgtcacgcat
    11701 ggggtcgcgg gttcgagtcc cgtccggacc gcctcttaaa agccacttca aaataggagt
    11761 ggctttgttt ttttggataa gtttcctctc agattttggt taatgtattt catacatttt
    11821 tcttaaagag aatttcaata aattgatatt caatgtattg tatcaggttc ttctgaccat
    11881 atttaaaatc aatttttgtt gcccaaacaa agcttctgta aagtttaaat tgctgatagc
    11941 ttatttttaa tcagattatt ttctttgctt aatgtaaatt tcagttattg aaatatactt
    12001 ttaaaaatta attgctgatt tgatactcaa taaaatcagt tttgtttttt ctaatataca
    12061 atccctaata tgattgatgt aattaattta aagaaaagtt atggcgagcg agaagcagta
    12121 aagtcattaa actttttgat taaagatggt gagttttacg gtcttttagg ccctaatggt
    12181 gcaggtaaaa ccacaaccat ttctataatg agtacaattt tagatgctga tgccggagat
    12241 gttgtaattg ataactataa catcaagaaa aatccaaaaa aagttaagca agtcataggt
    12301 gttgtacctc aggaaatagc gctctacaat gaactttcag ccggtgaaaa tttaaaattc
    12361 tggggaggtt tgtataacgt tcctgaaagt gagctgaaag caagaatgga tgaactgcta
    12421 aaatggtttg gtttatatga tcggagaaat gataaagtgg gtagttattc aggtgggatg
    12481 aaacgcagaa taaatattgc agcagctttg ttgcatcgtc ctaaagtact atttatggat
    12541 gagccaacgg ttggtattga cccgcaaagc agaaattgta tttacgaagt gattgaaaaa
    12601 ctccatcgtg atggaatgac tattgtttac acaacgcatt atatggaaga agcagaacgt
    12661 ttctgcgata gaattggaat tattgatgag ggaagaatta ttgccgaagg cacgttagat
    12721 gacttaaaga agtcgaaggc aattaaggag gttatctcaa ttactttttc aaagttaagt
    12781 gatacacttg ttaacgaact tacttcattt tggagagacc tgaaacatca ggatgataaa
    12841 attctttttt attcaaatga tataaaaaca gatttgccca agttggtttc aaagtgtaat
    12901 caattgagtg ctgatattgc caacattgaa attcaaaaaa caaatctcga aaccatcttt
    12961 ttaagtctaa ccggtaaaca attaagagat taatatgata aagcttgcta taaaagattt
    13021 aatgttattt attaaagaca aaaggtcaat ggtgctgact tttgctatac caattgcttt
    13081 gataacactt ttctcatttg cctttggtgg tgcaggaaag aacaaagaag atcagaaaat
    13141 aacactttta ctcagtgatt tggataaaac aaaattgtcg gaagctgcaa ttgaaaagtt
    13201 agactcgtta aaatctattc aaatagtaca aatacaatta gattccgcgc agaatgcaat
    13261 taaaaaagga aacgagagtt gcgtgctaat tattcataag ggttttgcag attctctttt
    13321 ggccggcaat gtattgccat tagagttgca atatgatgag gccagagata ttgaagtagg
    13381 gttgcttcag caatcgctta tacctactgt tgcaatgctt ccattcgatt tgggaaatac
    13441 gcagcaagtt atggccaata agtttcagaa aatggttggt aattcaagtc agaattcaaa
    13501 agcaaatatt cagatgaagt ctgatagttt gtttagtgca atttatcagg gaatggcaaa
    13561 caataatacc ggtggaggag ctaatgcaag tgatgctttt tttgatagcg gaataaagat
    13621 gacaaaactc attgcatcaa aaaatgataa tgcattagga ttgattcatg cagttgcagg
    13681 aacttctgtg atgatgcttt tgttttcagt ggttggaata ggggtagggc ttttagacga
    13741 aaaacaagaa ggaactttaa agagattact ttattcaccc attgacccta ttcatatttt
    13801 atttggaaaa ttaattactg ccaatataat ttcaatattt caattagtag tgatgtttgc
    13861 attttcaggc ttggtatttg gtttggatgt cagtgctcac ttgccgggtt taataatagc
    13921 aattgttgcc acggcatttg cctgttctgc atttggtgtt tttcttgcat cattcgctaa
    13981 atcaagacaa caagtacaag gactttctac tttaattatt ttagtgatga gtgccattgg
    14041 tggaagtatg atgccgctgt tttttatgcc tgcatttatg caaaaattag ccgttgtttc
    14101 agtaaattac tggagtattc agagttttta tgatattttc tggcgtaacc tatcaattac
    14161 aagtgttaca tttttgtcga gaataggagt gttgttatta ataggaacta tactcaatat
    14221 aattgctgtc tatttgttca ggaaaaatat tcttaaactg acttgatata atgaagccat
    14281 aaaatgttta cctgtaaatt aacttacttc attaatcatt ccctggctca cttcaaagag
    14341 tttaatgttt actgaaatgt cttcgaaaat tttttttaac ctttccggat gcgtgtcagt
    14401 aataaaaatc tgaccaaatg tgtgacggct gactagttcc atcagtcttt ttactctaaa
    14461 gtcgtctaac ttatcgaaaa tgtcatcaag caataaaatg ggttttgttt ttttgtggcg
    14521 aaacaaatat tcaaactgcg caattttaac tgccatcaaa aaagttttct gctgtccttg
    14581 cgaagcaatt ctttttagtg gatattttcc aagtttaaaa accaattcat ctttatgtat
    14641 gccggcagaa gtgtgttgaa gtgcaatatc tttttgtaga gataatttca ataatgtgcg
    14701 aaaatcgtct tgctcaagtt gtgtttgata atttattgaa acttcttcgg catgatcggc
    14761 cagataatta tacgtctcat taaacaaagg aataaattca gcaataaatt ttcttctttc
    14821 tttttcaatc gcacttccgt aatttactaa ctgttcatcc cacacttcca tcatagatgg
    14881 gtcgggcatg cgcccatgca attgtttcag atagctgttt ctgtgcatta gtgctttgtt
    14941 gtagctaatc aggttttcga gatatggttt attgacttgt gaaatgatac tgtcaataaa
    15001 ctttcgcctt tcatcactgc cttctgcaat cagaatatga tcaacgggtg ctaccatcac
    15061 aataggaaaa aggccgatat gatcacttag cctttcatat tcttcattgc ctcttttaac
    15121 ttgtttttta aatccggttt ttactccgca aaaaacatct tcttcttttt cgttaacaac
    15181 aaattttccc tgaatgacaa acagcgattc accatgatgg atattttgtg tatcagaaat
    15241 attaaaatag cttttcgtga aagagagata ataaacagcg tcaataatat tggtctttcc
    15301 actaccatta ctgccggtaa aacagtttac accggaagaa aaatttaatt gcgcttcagt
    15361 gtagttttta aaatttacaa gagataggtt agaaagatac acttattgca gacaaattga
    15421 ttgatggaat tattttttca gatattgttt atttacatca tcccatgatg gaaaatcatt
    15481 gtaatgccac atggcaacta ctacaccatt ttgcagcagt accaatcccg gatttgaacg
    15541 gatgatggtc ttcaatgttg ttccatcaca gaaataataa tcaaacatgt tttggttttc
    15601 atgacggaat aaatctgttt ctgctgcaac ggtagaggtc aatccaaaaa actgaacttt
    15661 attcttgcta caggcatcag caagctgatt tactttactt tgaatttttg tattagactt
    15721 attcagatca taagcaacaa gcataaagga atattgtgga ttatttaaaa attcctgagt
    15781 atgatcatgg ccgtctgcat caacaatagt aaaatcatgt attttaggct tatcaccttc
    15841 acgaattact ttgtctgtac gatcaacaaa ctcatagttt tccaaatctt ccggcaggtt
    15901 gttcatgcca aattctttta ttgtaccatc tttcgctttg tatttgaaaa caataaccac
    15961 actgtccgga cgtgcattgg gtggcggcaa catacctatt ttaaggttat ttccaattgc
    16021 ataaggtctg aaatcaatta caggtaagtg tgcataggtg tagtatgtaa aaagcgaact
    16081 ggcaaataat gcaaagatga caacccaacg tgtcttttta agttcaaaca acggtttaac
    16141 tttattgcgc catgcgaaga ttaccagaat aagtactaaa agcactacat cttttccaaa
    16201 cgattgccat ggagttagtt ttaatgcatc accaaagcaa ccgcagtcct taacaacatt
    16261 gaaataagca gaataaaaag tcagaaaggt gaaaaataca atcatcagca tcaataacca
    16321 ggtcactaaa ttcatgcgcc agcctactaa ggtagcaaca ccaagaacaa tttctgcaac
    16381 acagataaac tgtgccaatg ccacagaaaa tgcactcata aacgacatgt tgaatactaa
    16441 aaagtattca tcaagtttgt agctgaagcc aagagtgtcg ttggccttaa taaaacctga
    16501 aatgatgaat aaaacaccaa ctaaaattct acaaagtgta acaatatatt tcatactttc
    16561 tgtttgttat taatgtcttt taaatcaata caaataatgt tgttttatgc caattgtttt
    16621 caataattga caaaaacttt ttcacacctg atgtcaattg atatttttgc tctgtaaatc
    16681 agtatatgca aacatttact tctataaact gctgtggcaa attaatcaat ctgcataaac
    16741 ctcatgttat gggtgtatta aatcttacac cggattcttt ttatgatgga ggtaaataca
    16801 acagcgaaaa tgcaatttta acacaagtag cattaatgct gaatgaaggc gcaacattta
    16861 ttgacattgg tggttcttct tcgcgtccgg gctccaaaat gccgactgaa gaggaggagt
    16921 ggagcagaat aggaaatgta attgcattaa ttaaacaaca ttttggggat gtattgattt
    16981 ctgtagatac tgtttattca gtaattgcaa aacgtgcagt tgatgcaggt gcatgcatga
    17041 ttaatgatat ttctgccggt ttaatggaca gtaaaatgat tgaaacagtt gctgcactta
    17101 aagttcccta tttgatgatg cacatgaaag gccgacctga aaatatgcag gacaaccccg
    17161 tttatgataa tgtgcttaaa gaggtcattg attttttttc aaagcagtta ttgttgtgca
    17221 ggaaagttgg cataaatgac attttaatag acccgggttt tggttttggc aaaactgtcg
    17281 aacataattt tgaactgtta gccaaactta atttgctgaa gattgtagga cttccaattg
    17341 tagccggttt atcaagaaag gggatgatat ggaaaacact tgatataact ccttcagagg
    17401 cattaaatgg tactactgca ctaaacatgg ttgcattaat gaatggagcc agaattttaa
    17461 gagtacatga tgttaaagaa gccatgcagt gcataaagct gtttaataaa ttaaatgagg
    17521 taagataaat attgtcggaa caactaagcc aattctgcaa ttacagtaac accacctata
    17581 gttttctgat tgagttctac attaattttt gctgtcagtg gcagataaat atccacacgc
    17641 gagccaaatt taatgaaacc catttcggca ccttgtttta ccaaatcgtt ttctcgagga
    17701 taataaacaa ttctttttgc aagggctcct gcaatctgac gcaataaaat agttttgttg
    17761 gaatcttttt caacaacaat agtggagcgc tcattttctg ttgacgcttt cggatgccag
    17821 gcaactaaat atttcccttt atgatagcgc aaaaatttta ttttaccact catcggatac
    17881 cagttggcat gtacatttat gggtgacata aaaatagaaa cctgaatacg tttatcctta
    17941 tagtattcac tttcttcagt gcgttcaatt actacaactt taccgtcagc aggggcaatg
    18001 acataatttt cattacgtgt gatatttctt ttcggataac ggaaaaactg aagtatcagt
    18061 aaaaagaaaa ctatggttac tattaaaact aaaactgtta tccaatcgtg ttctgcacga
    18121 aaattaagta caagaaaatt gataaggagg acagtgatga gtgtccccca tacccaattt
    18181 tttccttcac ggtgaatagt catttttagt ggattagtag gcggtatgtt ttattaataa
    18241 tgaccgcgag atttaattcc taattcgatt ttgccaagtg cataaatata tgcagcaata
    18301 cgcatggtag tcttatgttt cacagaagta ttccaaacat tgtggaaagc ttgtttcatc
    18361 caatgatcag cttttgtgtt tacttcttct tctgtcatat ataatcctga acggttttga
    18421 ccccattcaa aatatgatac agttacacca ccaccgtttg caagaatatc aggaattaca
    18481 actgtacctt ttttgctcag aatgtgatcg gcctcatcag tagttgggcc attggcacct
    18541 tcaacaatta attttgcttt aatctttcca gcgttctcat cagtaatctg attttccatt
    18601 gcacatggtg ccagcacatc tacattaagc gtcagtaact cttcgttaga gataagttta
    18661 cctcccttaa atcctttaat tacaccttta ttagagtttc tgtatttaat ggctttttca
    18721 atatcaattc catcaggatt gtagaaagct gctgtatgat cagaaattgc tacaatcttc
    18781 aatccttttg cttcgtaatg ttttgccgta attgagccca catttccaaa gccttgaacg
    18841 gcagcacggc atttagcagg atttaatccc atttttgcca tagcttccat tgtggcagtc
    18901 ataactccgc gacctgttgc ttccgaacgt cctaatgagc caccaagatg tataggtttt
    18961 cctgtaacta cacccggagt aaaacctcct tttaacttac tgtattcatc aacaatccat
    19021 gccatttcct gagggcctgt attcatatca ggtgcaggaa tatccttgtc cacaccaaac
    19081 acatcaacca tagcattggt atatgctctt gtcaaacgct ctaattcacc tttcgacatc
    19141 gtagatgggt cgcaagtaat tccacctttt gcaccaccat aaggaatatc agcaacagca
    19201 catttaaagg tcatccatgc agcaagtgcc atcacttcgt tttcattgac ataagtgctg
    19261 tatctcacac cacctttcga gggtcctaaa gctgtgctgt gaacaactcg aaaaccttca
    19321 aatacttttg ttttatcatt atccatttta accggcatgc tgcaatacac aagcttgctg
    19381 ggttttttca ttatttcata aacttcagag tctaatttca tgatttttgc tgctacatct
    19441 aagcgcttaa tcatttcttc gaatggattt tcctcttcgt gattttgttt tgctttttta
    19501 ctatcagcca tgtcttatat aatattgatt gggaacgcaa aagtaggaaa gatttatgtg
    19561 tttgcaaacc atgcaggaag gctgtacttt tttataggtt ttgcatgaga tatatcagtt
    19621 tatcgtatgt aaatgcaagt ttaaatatta ctttacttac tttttagaat agtagccaat
    19681 gtcaattttg acattcagtt gacaatgcac tttttatcaa tgaaaaatct atctgaaaaa
    19741 cactattgaa tgatgtttac ttcaggctca atatgaactc cgaaatggtt gtaaacagat
    19801 tctttaattt ttaatgcgag gctatagatt tcagcacctg tagcattacc atagttcacc
    19861 agtactaaag cttgattttt atgacatcct gcatcaccct ctctaaatcc cttccaccca
    19921 cattgctcaa taagccatcc tgctgccagt tttattttgt ttgcagctcc cggataatgc
    19981 ggaatatttg gatgttgttg cttgattttt tcaaattcct gctcagtaat ttcaggattc
    20041 ttaaaaaaac tacctgcatt gccaataaca gcaggatcgg gtaatttact tcgtcttatt
    20101 gctattaccg catcactgat ggctttaaca ctaagatttt taattccttg tttttcaagt
    20161 tccgagtgaa tggcaccata ttcagtttta aattgaggct gctttttcaa ttccagaata
    20221 atagctgtga taatgtattt tccttttgct gcatttttga aaacactatt tctgtagcca
    20281 aattcacatt catggttatt aaacactttt ttttctgcac tatctattgc taatgcttca
    20341 acagctacga gtgagttttt tatttctaca ccataagcgc caatattctg aattggtgca
    20401 gcacctgcag tacccggaat cagcgaaagg ttttctatac ctccaaggtt tttttctacc
    20461 gaccacatga caacattgtg ccatacttca cctgcaccaa ttcttatttt aacggaagct
    20521 tcgttctctt caactacgtc aatgccgtgc aggttgtttt ttaaaactat accatcaaaa
    20581 tctttaaaaa aaagcatgtt gctgccacca cccattacca ataatggaag tgttgttttt
    20641 ttatgctgaa gaagaaaaag tattaagtcg tcctcactgt gtatatcaat ataatagcgt
    20701 gcgtttgctt caatgccgaa ggagtttaaa ttctttaatg aaacattttc acgcataggc
    20761 atcagttgac gatgaattta ccaatagcaa taatttcatt tgccgacatt atttttacat
    20821 aatacagtcc agcttttaat ccattgtttt gtataatgaa gacttgttta ttttcagtat
    20881 tacctgcatg gttataggga atatttacta ttcttcctgc tacatcaaaa aagtataatt
    20941 taaaatcttt tgattctgca gttgtaatac ttaagtaagc tttgtcagca aatggattcg
    21001 ggaaaatttt aatttctttt atttccggtg aaaggctgtt tattgcagta gcttcgtcaa
    21061 caaaaacact atcacattgc atgcaattgt tgaagtcctg tgcacaaaat aaatagtaac
    21121 ccataccaag attggttgct gctgaatctg caattcctaa tgctggatac aaataatcat
    21181 acggtggtgt gccacctgtt gcagttgcta taatgctgcc atctgtacaa cctgggcaag
    21241 aggcatcaat tactgtaaaa ttgataacta ttggatcagg ttcaacaaaa gtaaaagagt
    21301 caacagctgt a
//
LOCUS       DAOOOL010000033        21129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_192_length_21129_cov_140.335817, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000033 DAOOOL010000000
VERSION     DAOOOL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21129
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192_length_21129_cov_140.335817"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKU82_04380"
     CDS             <1..500
                     /locus_tag="PKU82_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOZ82144.1"
                     /translation="STYLAAGIEKAIVIVNPDIEREVSVLLKNKFSDSKLEVVVNSYP
                     EKGRFYSIQLGLEKTDTDSCFIQNTDNPFVTKDLLNNMCRMIKQGAYVVPVFKNEKGH
                     PVIISAEVMNHCRKLNGNDFNLRDELQMFEEIRLVWDEKNILANINTEDEYQKYFPSC
                     EAFTK"
     gene            479..1315
                     /locus_tag="PKU82_04385"
     CDS             479..1315
                     /locus_tag="PKU82_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOZ82145.1"
                     /translation="MRSIYEIIAGLEQCGEEAALCIITETRGSTPLKAGAKMIVHRNK
                     NISGTIGGGNLEKQVIEDAVSVMESGKAATFEHQLVRDHQMCCGGSVFVFIEPVKKRN
                     KLIVFGAGHVGKEVAKLACSLNFKISLVDERKEMLTGLALDDAKIYNMHHLQFLSDVV
                     LDENTFVVICTHLHHYDREILARCIKQPYAYLGMIGSMRKVVITRKKFISEGLASEEE
                     LSKVDMPMGFDIGGNSPAEIAISIVAKLVAVMNGHTMQNNNEEKSNYTYAGQDSDNNR
                     CW"
     gene            1278..2015
                     /gene="fabG"
                     /locus_tag="PKU82_04390"
     CDS             1278..2015
                     /gene="fabG"
                     /locus_tag="PKU82_04390"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOZ82146.1"
                     /translation="MPDRIAIITGAGKGIGKAIAERLSADGFYVALVDVDQAAMEEAV
                     AIIGADKATGYLVDITNENQVKALFENIENAKNKIDLLVNNAGIIRDNLIWNLSANDF
                     DAVINVNLKGTWLMCREAAIRMKQQKYGCIVNIASRAWLGNAGQTNYSASKAGIIGLT
                     RSLALEMGRHNVSVNAIAPGLIDTPLTQKLDKDVLDKLIQAQPSRSMGKPEDIANVVS
                     FLASEKTSFMTGQTIYVDGGKSIGAGL"
     gene            2043..4838
                     /locus_tag="PKU82_04395"
     CDS             2043..4838
                     /locus_tag="PKU82_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOZ82147.1"
                     /translation="MRSIKIKVNGRQHEVLIDEHETLAHVLREKIELTGTKIGCEQGS
                     CGACTVLLNDEPVLSCITPAMRCDELSVTTIEGVSENGELHLLQQKFVEKGAIQCGFC
                     TPGMVMTSLDFVRKNPHATVDEIKEALSGNLCRCTGYKKIIEAVAETIRPQQHPAEAN
                     GSKPHNGRIVGTGMPYIEAAKKVQGKAEYADDIRLKNVLHCSFLRSPYPHAKIEQIDT
                     SAAAKLEGVKYILTGVELPEKFGVLPISQDETALAIDKVRYIGEVVAAVAAETEAIAK
                     AACNLIQVKYKPLKPFFEIDESLNDIGTEEKIHEHVKFNNNVHKKAELRFGNQEEGFQ
                     SAAFKSKMNFEFEGITHGFTEPHAATAYWDENGLTIITATQVPHYLHRALSKVLKLPL
                     SRVRVIKPYVGGGFGGKSDPFPHEIIVSYLAIKTGRPVKVRFSREEVFISNHGRHPTK
                     MTMEIASDETGNFRALDADIAIDGGAYGSFGVVTTYYNGVLLQAPYKVENFGFKTFRV
                     YTNKPQCGAMRGHGAVNSRFAIETLIDDLAHQLKMDPCELRLKNFMPSNTLTAGQYRI
                     TSNGSRESLLKVMEQSQWKERYGKLPFGHGLGVGCGFFISGSALPIIRNELPQSVVHL
                     KVDFDGRVLVTSGASDIGQGSDTMLAMIVAEVLGISMDKIFVLAADTLLTPVDLGSYS
                     SRVTFMAGNAAKMAAENLKKEIINSVVARKEISADELNLADDCVFSNDRKIELTWNEA
                     IDFTIAHRGAINVSGKYISPKLGGDFKGSGAGLSPSYSFGAVVTEVKVDPETGAVKVL
                     NIWGAHDCGKAINPLAVEGQLEGSWHMGLGQAMSEQVKYFNGLLINSNFLDYKIPTSL
                     DTPPIHTNIIETADPEGPFGAKECGEGAIHPIIPAVANAVFDAVGARITKLPISPEDV
                     LQKIKNLSLPNAAQVNV"
     gene            4847..5779
                     /locus_tag="PKU82_04400"
     CDS             4847..5779
                     /locus_tag="PKU82_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOZ82148.1"
                     /translation="MSDNKKYLKPVSVHEAITMSKVCGDDFRYIAGATDVMVNRRQGN
                     EESFCLIDLCGIDELKQVTVKENYLSIGSLVRLDDLKKHAAIVKEFPVLIEAAETVGS
                     PLIRKSATLGGNVLCENRCIYYNQSEWWRDAVGYCLKCEGNICIATGSKKLCYSEFVS
                     DTAPALISMDAEIEIADHEGERTLKLEDIYTGDGVCPRNLKKSAVIKSILLPLQREFH
                     SSFHKLRQRQSLDFTSLTVAVSIDKNGKLKSVLGGVDPKPVVVEGNSDSNLEEIIKLA
                     LKGSRAIDNEMFSRNYRRSMISVFLKRSFEKLIG"
     gene            5787..6374
                     /locus_tag="PKU82_04405"
     CDS             5787..6374
                     /locus_tag="PKU82_04405"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOZ82149.1"
                     /translation="MISDKSIEVFVLAGGKSSRMGEEKGLMLLHNKPMIQYVLEQLSI
                     LKLPVQIIANADDYTKFGYDTLKDIIAEKGPMGGLLTAFHYAKSDCVLLISCDMPFIT
                     SEIVSKLIFEVKDDEITAAYVQNEPNPLFAVYKKSLKNEVEKRIAAGNLQMKHLIKSF
                     RHKRVEMDVYLNRNPNLFINLNSKQDVNNITDVWN"
     gene            6365..6607
                     /locus_tag="PKU82_04410"
     CDS             6365..6607
                     /locus_tag="PKU82_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOZ82150.1"
                     /translation="MELKGIKILAFGMIAEKINASEFYLKEVSNTNELLSSLNSLYPQ
                     LKEMKFTISVNRRIVNNNEPLNADSEVALLPPFSGG"
     gene            6618..7715
                     /locus_tag="PKU82_04415"
     CDS             6618..7715
                     /locus_tag="PKU82_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOZ82151.1"
                     /translation="MSRFERQINLSGFGPKAQQQLQKAKVLVIGAGGLGCPVLLYLDA
                     AGVGTLAICDGDVVAASNLNRQVIFSESDITKSKAEVAGIYLKQKYSDINIEIINKFL
                     TVENTLQIFNAYDLIIDGSDNFQTRYMVNDACVLLNKPLVFGAVYQNEGQVAVFNVSD
                     DNAVSSNYRDVFPNPPSENEILNCNETGVLGVLPGIIGVMMASEAIKLITGFGKALIN
                     KMLYYNLLTNTTYETEIAPNAVAKSIIPKTPEDFYKTDYTLLCGNLPSIEWSEALLLK
                     NNTPGKVILLDVRETHEQPKLDGIETMNIPLQSLSENIEPLNTAENILVFCQSGIRSR
                     KAVAVLRSKFLQKNIYSVAGGISKLNSTINA"
     gene            7721..8161
                     /locus_tag="PKU82_04420"
     CDS             7721..8161
                     /locus_tag="PKU82_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOZ82152.1"
                     /translation="MERKPKQIFIQGAISSSKIADSIQKHSSKTDIGAHSIFLGQVRT
                     DEKPEGIIQAIEYTTYEEMALQKAFEIREDIFAKYPLTCLHIYHSLGVVKTGEICLFV
                     FTSSKNRKAATEACNEMVERIKNELPVWGKEIIDEKKSLWKENS"
     gene            8168..9079
                     /gene="moaCB"
                     /locus_tag="PKU82_04425"
     CDS             8168..9079
                     /gene="moaCB"
                     /locus_tag="PKU82_04425"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HOZ82153.1"
                     /translation="MINITHKNSTHRTAIAQAIVKVSKEETITAVKENKVPKGNVFEM
                     SKTAGLFAAKRTSDMIPDCHPLPIEYAHITHEINGLEILIRVEIQTIYKTGVEVEAMH
                     AASVVALTIYDMLKPIDKNISIEQIKLIEKKGGKSDFIKKEISGIKAAVIVCSDSISK
                     GTKEDKAGKAIIEKLKSCKVDIADYIIIPDEKNIIQEKVRAYCSQQIQLVILTGGTGL
                     SPRDITPEALDSVIEKRIPGIEEAIRTYGQERTPYSMLSRSIAGLVGNTLVLALPGST
                     RGAAESMEAVFPHILHIFRVMKDARHD"
     gene            complement(9110..10750)
                     /locus_tag="PKU82_04430"
     CDS             complement(9110..10750)
                     /locus_tag="PKU82_04430"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOZ82154.1"
                     /translation="MAEKVNLEFNINEDAYKSHLIRLAERLNKIYEGGGKKNAAKQKE
                     KNKMLARERIAYLLDTDKPQFEVGAFAADGMYAEQGGCPSAGVVIVIGYISGKQCIVV
                     ANDATVKAGAWFPMTAKKNLRAQEISMENKLPIVYLVDSAGVFLPMQDEIFPDKEHFG
                     RIFRNNAVMSSMGILQIAAIMGSCVAGGAYLPIMSDEALIVDKTGSVFLAGSYLVKSA
                     IGEDIDNETLGGATTHCEISGVTDNKFPDDASCLDYIKRLFSKIGTFDKAGFERAESK
                     EPAANPKEIYGIIPAENSKQYDMHEVINRLVDNSEFDEYKEKYGQSILCGTARIDGWA
                     VGIVANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQRRIPLVFLQDVTGFMVG
                     SRSEHGGIIKDGAKLVNAMANSVVPKFTIIIGNSYGAGNYAMCGKAYDPRLIAAWPTG
                     QIAVMGGSQAAKTLLQIQVAALKAQGKEISEEDEKAQLKTITDRYNSQTSPYYAASRL
                     WVDAIIDPLETRNMISMGIEMANHNPITKPYNVGVIQT"
     gene            complement(10820..10996)
                     /locus_tag="PKU82_04435"
     CDS             complement(10820..10996)
                     /locus_tag="PKU82_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HOZ82155.1"
                     /translation="MAFTDKFEKDEYYLTPEGYIVFTEKYHLKRGYCCQSGCRHCPWN
                     YNTKKADKNNKQKS"
     gene            11124..12029
                     /locus_tag="PKU82_04440"
     CDS             11124..12029
                     /locus_tag="PKU82_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOZ82156.1"
                     /translation="MNSPIIDEVNLNDFQLAHKGVSLHVLRLDLDDVITGGNKSFKLK
                     YNLQAAKEKGRSIIVTFGGAFSNHIAATARTCKLQNLKAVGIIRGEIVTPMNNTLSRA
                     VNDGMTLIPVSRQWYRQIHDKEFYKSNLVKCLLIANNINPDACYVVPEGGNNAEGFKG
                     CLEIMNNINADYDTVVCAVGTGTTLAGIAASLPQNKNVHGIAVVLAADTLTKNIQLHL
                     AESNSNAKYIVHQQYSFGGFGKSNAELKTFCELFTQKTNILIEPVYTGKLFYAILDML
                     AKDKFNPGTKILAIHTGGCQYLIDK"
     gene            complement(12082..13662)
                     /locus_tag="PKU82_04445"
     CDS             complement(12082..13662)
                     /locus_tag="PKU82_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82157.1"
                     /translation="MRKHLQKLAVLGMFSMFAYTAQAQRFLTEVFPSVNVTPNVVYGQ
                     NYEVLTGTPVLKDLKMDIYEPAGAADPMTERPLIIYLHTGSYIPIIVNGNATGSRNDS
                     AAVEICKQFARRGYVVANMDYRLGWDPTNTNPNNGLDIRRGTLLNAVYRSIQDAKSCV
                     RYFRKDAATNNNVYKIDVNNFYLVGQGTGGYIALNYAVLNSPSEITLPKFISSTDAPT
                     YGIYAGQPYVNQTALGDFDGIGGVAPYNNENNPGYSNAVQFVVNLGGAMGDSSWIAAG
                     DAPMIGIHCVDDPFAPYGVGIVYVPTTPPQAVVDVIGSGEIIKWANQLGNNACFANAG
                     FTDPYTLRANSINNGNEGLFPLQQGINPVNPQLGHAGPWEWYDLQSTRNLAMAMGYQS
                     TRGDTCYGNSMLTNPTMSKTYALAYIDTIMNYVNPRIVFCRNLPTSVKEVSTNSSDVT
                     IYNSPSKSYFTVVSTSKEPMRSIEVYDIKGSLLASEKNPNAFNQRIEKASLSLNKGVV
                     LVKITYNSDVITKKITLD"
     gene            complement(13728..14372)
                     /locus_tag="PKU82_04450"
     CDS             complement(13728..14372)
                     /locus_tag="PKU82_04450"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOZ82158.1"
                     /translation="MNAKKEKELSPLDNITDFEVFISGRLKDLPGRNSHLKLASVNRP
                     VYEPEGNYRIGSVMLLVNNKNNIPHLVMIQRAEDGKVHSGQIAFPGGKAEPDESLTLT
                     ATRELEEEIGIADKSINIIGQLSRIYIPPSNYMVYPFIATQIQTSVFLANQNEVKEIF
                     EIPLHSLIKNKTIIQGLQHQTTRGLITAPAFRIGNLVIWGATAMILSEFIDLID"
     gene            complement(14335..16107)
                     /locus_tag="PKU82_04455"
     CDS             complement(14335..16107)
                     /locus_tag="PKU82_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ82159.1"
                     /translation="MKILVTYLKRHWKLVLLALLLAAVNQCFSLLDPLIAGKMLDRFA
                     MHVSNYSNDFPKFFQDIIWFLLASMGVALVSRIAKNFQDYFVNVIVQRTGAEMYSDGI
                     KHSLELPYQVFEDQRSGETLGKLQKVRADSEKFINAFISILFQSVVGIIFVMAYAINV
                     HWSIAPVFLLTVPVLAVVSNMLSKKIKVISKKILGETTALAGATTESLRNIELVKSLG
                     LANQEINRLNTVTDKILNLELKKVKFIRSLSFIQGTTVNFLRTLFVFLLYYLIFKDEI
                     SPGKMMTLMFYSFFIFGPLQELGNVIAVYREAQASLNNFSEIMEMPLEYKPQSPLQLN
                     AIETFEFSSVSFKHISAHGNAVNNISFKARRGETIAFVGPSGSGKSTLVKLLVGLYHP
                     AEGKILYNGIDANNINLNELREQIGFVTQDTQLFAGTIRENLLFVNPHAHDDQVLNAL
                     NMASCHSLLARAENGLNTVIGEGGVKVSGGEKQRLSIARALLRNPNLLVLDEATSALD
                     SITEEEITETIKSINKNKNLITILIAHRLSTIMHADHIFVLEKGHKIEEGRHETLLEE
                     KGLYYAMWRQQIGERKKGERVVTA"
     gene            16460..17539
                     /locus_tag="PKU82_04460"
     CDS             16460..17539
                     /locus_tag="PKU82_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HOZ82160.1"
                     /translation="MQKIVKLYEWTTSLPIITCLLYFSGLLDNNIVLQILAGILLLFC
                     VMSAVHHSEIVAHRVGEPYGTIILAVSITIIEVAIIISLMMTGGKEYASFARDTVYAA
                     VMLILNGIIGLSLFLGGRKYHTQTFSPHSVKIALVALVSIVTFTMILPTFTSSIIGPY
                     YTERQLLFEIIACLIIYIAFIAAQTIRYREYFITEANPDVEVTHNNISNSSFIISLIF
                     LMICLVIVVLLAKSLSNPIENLLTSYGLPRSLVGIVIAAVILLPEGIAAVSSARKNKL
                     QNSLNLALGSALASIGLTIPAVSLASYIFDLPLILGLELLPIILLAISIFTVMLSLIG
                     GRSNIVYGVVLLVNLFAYVFLTINP"
     gene            17931..18707
                     /locus_tag="PKU82_04465"
     CDS             17931..18707
                     /locus_tag="PKU82_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82161.1"
                     /translation="MFFSDAIGNKDTITLGYDLTATDSIDTAFGETNIISVPLDTNLD
                     VRITNAWFTYADAVYFHLKKQIVFYDCSLHNLNIQPINIVTQHWPVTMTWNNSIFNDS
                     CNNGSVFTGINPAGWWDTGCPSGLLQQVFLGDDSITFNSNFDLGWFGYINSNGDSIPV
                     FWQAFGDSTILTTGINELPQQCISVKVFPNPGSTYITVQANNSFGQLSLISILNSYGQ
                     IVLKTQQISRIDISELPDGIYFIKTENTKGEFTTSKFLKL"
     gene            complement(19010..19852)
                     /locus_tag="PKU82_04470"
     CDS             complement(19010..19852)
                     /locus_tag="PKU82_04470"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOZ82162.1"
                     /translation="MKKQFIFLTAILLFLLFNACRKNDNTDEVSPNVPDSTSITNSLG
                     TMPSVFEQRVLIEELTGEWCPGCVNGAHILQSIKDSAINGGKVDVVSIHDVDWLEIVK
                     YDTVRFHYWQAMFGFPCATVGRVVQPSTGTYYVDYTKWQSNVTSRLAETHDLGIALIS
                     KLENDSGRVEVHIGHNSHINDNLRLTIFVTEDNVLAQNQLGSVGNFFHQQVVRLMPTN
                     ALGDDITFTSTETVMSYSFPVVGYKLSDLKIVAMVSSWNYNPIQSKIWNSRTVKFGQI
                     AKWK"
     gene            complement(20252..>21129)
                     /locus_tag="PKU82_04475"
     CDS             complement(20252..>21129)
                     /locus_tag="PKU82_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOZ82163.1"
                     /translation="KFPELIKDIKGLSEETTTGVHRLYERMQKGTLPIPAININDSVT
                     KSKFDNKYGCRESLQDAIRRATDLMLAGKVAVVAGYGDVGKGSAESLSSAGVRVIVTE
                     IDPICALQAAMEGYEVKTMDNAIPEADIIVTATGNHNIIVDKHFKAMKHNAVVCNIGH
                     FDNEIDMAWLNKNYGNSKDTIKPQVDKYTLDGKDIIVLAEGRLVNLGCATGHPSFVMS
                     NSFTNQTLAQLELWNHTDKYENKVYTLPKHLDEKVARLHLSKIGVELETLTQSQADYI
                     GVPVAGPYKPDYYRY"
BASE COUNT         6743 a         3979 c         3994 g         6413 t
ORIGIN      
        1 gaagcacata ccttgcagca ggtattgaaa aagcaattgt aattgtaaac cctgatattg
       61 aaagagaagt cagtgtatta ttgaaaaata aattttcaga cagcaagctt gaagttgttg
      121 taaattcata tccggagaaa ggaagatttt attccataca actcggattg gaaaaaacgg
      181 atacggattc ttgttttata caaaacactg ataatccttt tgtaaccaaa gatttgctga
      241 ataatatgtg ccgaatgatt aagcagggtg cttatgtagt tcccgtgttt aagaatgaaa
      301 aaggtcatcc tgttatcatt agtgcggagg taatgaacca ttgcaggaaa ttgaacggaa
      361 atgattttaa cctgcgtgat gaactgcaaa tgtttgagga aataaggtta gtttgggacg
      421 aaaaaaatat tttagctaat attaatacgg aagacgagta tcaaaaatat tttccttcat
      481 gcgaagcatt tacgaaataa ttgccggtct tgaacagtgt ggcgaagaag cggctctctg
      541 tatcatcaca gaaacccgtg gatctactcc gcttaaagct ggcgccaaaa tgattgtgca
      601 ccgaaataaa aatatttccg gcaccatcgg tggcggcaac ctcgaaaagc aggtgataga
      661 agatgctgtg tccgtaatgg aaagtggcaa ggctgctact tttgaacatc aattagtccg
      721 tgaccatcag atgtgttgtg gcggttcggt gtttgttttc attgagccgg ttaagaagag
      781 aaacaaactg attgtattcg gtgctggtca tgtaggtaaa gaagttgcga aacttgcctg
      841 ctcactgaat tttaaaatca gccttgttga tgaaagaaaa gaaatgctca ccggactggc
      901 attggatgat gcaaaaattt acaatatgca tcatctgcag tttttgtctg atgtagtttt
      961 ggatgaaaat acattcgtag tcatctgtac tcacttgcat cattacgaca gggaaattct
     1021 ggctcgctgc atcaaacaac cttatgctta cttaggcatg attggcagca tgagaaaagt
     1081 agttatcaca agaaagaaat ttataagcga aggattggca agtgaagaag aactttcaaa
     1141 agtagatatg ccaatgggtt ttgacattgg cggaaattcg cctgctgaaa ttgccattag
     1201 cattgttgca aaattggttg ccgtaatgaa cgggcacact atgcaaaata acaacgaaga
     1261 aaaatcaaat tatacgtatg ccggacagga tagcgataat aacaggtgct ggtaagggaa
     1321 ttggcaaagc cattgccgaa cggttgtcag cagacggctt ttatgtggca ttggttgatg
     1381 ttgaccaggc agccatggaa gaagcggttg caatcattgg cgctgataaa gctaccggtt
     1441 accttgttga tatcaccaat gaaaaccagg taaaagcatt atttgaaaat attgaaaacg
     1501 caaaaaataa aattgatttg ttggtgaaca atgccggaat tatccgtgat aatttaatct
     1561 ggaatttatc ggcaaatgat tttgatgcag tgatcaatgt caacttaaaa ggaacatggc
     1621 tgatgtgccg tgaagctgcg atacgaatga agcaacagaa gtatggctgt attgtgaaca
     1681 ttgcttcccg tgcatggctt ggtaatgcgg gacaaaccaa ctactccgct tcaaaagccg
     1741 gtatcatcgg gcttacacgt tccttggcat tggaaatggg aagacataat gtttcagtta
     1801 atgccattgc tcccggttta attgacacac cacttaccca aaagttagat aaagatgttt
     1861 tggataaact gattcaggct caaccctcac gaagcatggg caagcctgaa gatattgcta
     1921 atgtggtttc atttcttgct tcagaaaaaa caagtttcat gaccgggcaa accatttatg
     1981 ttgatggcgg aaaaagtata ggggctgggt tataaaataa ttgcagtaat aaatattgaa
     2041 taatgaggtc aattaaaata aaggtaaacg gaaggcagca tgaagtgttg attgatgaac
     2101 atgaaaccct tgctcatgtg ctgcgggaaa aaattgaatt gacaggcaca aagattggct
     2161 gcgaacaggg aagttgcgga gcctgcactg ttctgctgaa tgatgaaccg gtgctcagtt
     2221 gtattacacc agccatgcgt tgtgatgagt tgtctgttac caccattgaa ggcgtttctg
     2281 aaaatggtga attgcatttg cttcaacaaa agtttgtaga gaaaggcgcc attcaatgcg
     2341 gcttctgcac tccgggaatg gtgatgacct cattggactt tgtaaggaaa aatccccatg
     2401 cgaccgttga tgaaatcaaa gaagcattat cgggaaatct ttgccgctgc acgggttaca
     2461 aaaaaataat tgaagccgtt gctgaaacca tccgtccgca acagcatccg gctgaagcaa
     2521 atggtagcaa gccgcacaac ggcagaatcg ttggaacggg aatgccttac attgaagcag
     2581 ctaaaaaagt gcagggcaaa gcggaatatg ccgatgacat tcgtttgaaa aatgtattgc
     2641 attgcagttt tttaagaagt ccttatccac atgcaaagat tgagcagatt gacacaagtg
     2701 ctgcagcaaa actggaaggt gtaaagtata ttctcaccgg agttgaactg ccggaaaaat
     2761 tcggagtgct gcccatttcg caggatgaaa cagcgttggc aattgataag gtaagataca
     2821 ttggcgaagt ggtggctgca gtggctgccg aaacagaagc cattgcaaaa gctgcctgca
     2881 acctcattca ggtaaaatac aaaccactga aaccgttttt tgaaattgat gaatcactaa
     2941 acgacatcgg cacggaagaa aaaatccatg agcatgtgaa gttcaataac aatgttcaca
     3001 aaaaagcaga gttgcgtttt ggaaaccagg aagaaggatt tcaaagtgca gcatttaaaa
     3061 gcaaaatgaa ttttgaattt gaaggtatca ctcatggttt cactgagcca catgccgcca
     3121 ctgcatattg ggacgaaaac ggtttgacca tcattactgc cacacaggtg cctcactatc
     3181 tccacagggc tttgagcaaa gtattgaagt tacctttgag cagggttcgt gttattaaac
     3241 cttatgttgg cggtggattt ggcggcaaga gcgatccttt tccgcatgaa ataattgtgt
     3301 cttacctcgc cataaaaacc gggcgaccgg ttaaagtgcg tttcagccgt gaagaagttt
     3361 ttatcagcaa tcacggacgg catcccacta aaatgacgat ggaaattgct tcggatgaaa
     3421 caggaaattt ccgtgctctc gatgctgata ttgctattga tggcggtgct tatggaagtt
     3481 ttggtgtggt tactacttat tacaatggtg tgttgttgca ggcgccttat aaagttgaaa
     3541 atttcggctt caaaactttt cgtgtttaca ccaataagcc gcaatgcggt gccatgcgtg
     3601 gacatggcgc tgtgaattca cgctttgcca ttgaaacgct gattgatgat ttagcgcatc
     3661 aattaaagat ggatccgtgt gagttgcgat taaaaaattt catgccatcc aatacactca
     3721 cggcaggtca atatcgcatc acttcaaacg gcagtcgtga atcgttgctt aaagtgatgg
     3781 agcaatcgca gtggaaagag agatatggta aactaccttt cggtcatgga cttggagtgg
     3841 gctgtggatt ttttatcagc ggcagcgcat tacccattat caggaatgaa ttgccgcaat
     3901 cggtggtgca tttaaaagtt gatttcgatg gacgggtgtt ggttacttcc ggtgcaagcg
     3961 acatcggcca gggaagcgat accatgcttg ccatgattgt tgccgaagtg ctgggcattt
     4021 cgatggataa aatttttgtg cttgcggcag ataccttgct tacacctgtt gacctcggca
     4081 gttattcaag ccgtgtaact tttatggcgg gcaatgcagc gaagatggca gcggaaaatc
     4141 ttaaaaaaga aattatcaac tcagtggttg cacgaaaaga aatttcagca gatgaattga
     4201 accttgctga tgattgtgtg ttttcaaacg atagaaaaat tgaactgaca tggaacgaag
     4261 caattgattt taccattgca catcgtggcg ctatcaacgt tagcggaaaa tatatttctc
     4321 caaaactcgg tggcgacttt aaaggctcgg gtgcaggatt gagtccttcg tatagcttcg
     4381 gagcggtagt tacagaagtt aaagttgatc cggaaaccgg tgccgtgaaa gtgttgaata
     4441 tttggggcgc acacgattgc ggaaaagcaa ttaatccatt ggcagtggaa ggacaattgg
     4501 aaggctcgtg gcacatggga ttgggacagg caatgagcga gcaggtaaaa tatttcaacg
     4561 gcttactgat caacagtaat ttcttagatt ataaaattcc cacttcgctc gatacaccgc
     4621 ctatccacac caacatcatt gaaacagcag atcccgaagg gccatttggc gcaaaagaat
     4681 gtggcgaagg agccattcac ccgataattc ctgctgttgc aaatgcagtg ttcgatgcag
     4741 tgggtgcaag gattacaaag ttgcccatca gccctgaaga tgttttacag aaaattaaaa
     4801 atctttcact tccaaatgca gcacaggtaa atgtgtaaga ataaaaatga gtgacaacaa
     4861 aaaatattta aagccggttt ctgttcacga agctataacc atgagcaagg tatgtggaga
     4921 tgatttccga tacattgcag gcgcaacgga tgtgatggtt aaccgcaggc agggaaatga
     4981 agaatctttt tgcctgattg atttgtgcgg cattgatgaa ctgaagcagg taacggtaaa
     5041 agaaaattat ctgtcaatcg gttcgctggt gaggttagac gatttaaaga aacatgcagc
     5101 catcgtaaaa gaatttcctg tgttgataga agcagccgaa actgtgggtt cgcctctcat
     5161 caggaaatca gccacattag gcggaaatgt tttatgtgag aatcgttgta tttattacaa
     5221 tcaatcagag tggtggcgtg atgctgtagg ttattgcctc aaatgcgaag gcaatatctg
     5281 tattgcaaca ggcagcaaaa aactttgcta ctccgaattt gtttccgata ctgcgcctgc
     5341 attaatcagc atggatgcag aaattgaaat agccgaccat gaaggagaac gcaccctgaa
     5401 acttgaagac atttatactg gtgatggcgt ttgcccacgc aaccttaaaa aatcggctgt
     5461 catcaaatct atattgttgc ccttgcaacg tgagtttcat tcttcatttc acaaactgag
     5521 gcaaaggcaa agcctggatt ttacttcgct tacggttgcc gtttccatag ataaaaacgg
     5581 aaaactaaaa tccgttctcg gtggagtaga tcccaaaccg gttgtggtgg aaggcaacag
     5641 tgatagtaat cttgaagaaa taataaaact ggcattaaaa ggctcccgag ccattgacaa
     5701 cgaaatgttc tcacgcaatt accgcagatc catgatcagt gtttttctga aacgcagttt
     5761 tgagaagttg ataggatgac gcgattatga tttctgataa aagcattgaa gtatttgttt
     5821 tagccggagg caagagcagc cgcatgggtg aagaaaaagg actgatgttg ctccataaca
     5881 agcctatgat acaatatgtg ctggaacaac tatccattct caaattacca gtgcaaataa
     5941 tcgccaatgc tgatgattac accaaatttg gatatgatac gctgaaagat attattgctg
     6001 aaaaaggacc gatgggcggc ttgcttacag cttttcatta tgccaaatca gattgtgttt
     6061 tgcttatcag ttgcgatatg cctttcatca catcagaaat agtcagcaaa ctaatttttg
     6121 aggtgaagga tgatgaaatc actgcggctt atgttcaaaa cgaacccaat ccattgtttg
     6181 cagtctataa aaaatcactg aaaaacgaag tggagaaacg aatagcagca ggaaacttac
     6241 aaatgaagca tctcataaaa tcgttccgcc acaaaagggt ggaaatggat gtatatttga
     6301 atcggaatcc taacttgttt atcaatctga atagtaagca ggatgtaaat aatattacag
     6361 atgtatggaa ctgaaaggaa ttaagatttt agcgtttgga atgattgctg aaaaaataaa
     6421 cgcgagcgaa ttttatttga aagaagtgag caataccaat gaactgctga gttcgctgaa
     6481 cagtttatac cctcaactta aagaaatgaa gtttacaatt tcagtaaaca gaagaattgt
     6541 aaataacaac gaacctttaa atgcggacag cgaagtggca ttgcttccgc cattttcagg
     6601 aggataatta aaccataatg agcaggtttg agaggcaaat aaatttatcc ggtttcgggc
     6661 caaaggcaca gcaacaactg caaaaagcaa aagtgctggt gataggagca ggcggattgg
     6721 gatgtcctgt gctgttgtat cttgacgcag caggagttgg cacattagcc atttgtgacg
     6781 gtgatgtggt tgccgcttct aaccttaacc gtcaggtaat tttttcggaa tcggatatca
     6841 ctaaatcaaa agcagaagtt gcggggattt acctgaagca aaaatacagc gatattaata
     6901 tcgagattat caataaattt ttaactgttg aaaatacgct tcaaattttc aacgcttatg
     6961 atttgataat tgatggtagc gataattttc aaacgcgcta tatggttaac gatgcctgtg
     7021 tgctgctgaa taaaccactt gtgtttgggg cagtgtatca aaacgaaggt caggtggcag
     7081 tgtttaatgt aagtgatgat aatgccgtct cttcaaatta ccgtgatgta tttccgaatc
     7141 cgccttctga aaacgaaatt ctaaactgca atgaaactgg tgttttgggt gtattgcccg
     7201 gcattattgg tgtaatgatg gcttcggaag cgataaaatt aataacggga tttggcaaag
     7261 ctttgataaa taaaatgctt tactacaacc tgcttaccaa caccacttac gaaacagaaa
     7321 ttgcacccaa cgctgttgca aaaagtatta ttccaaaaac cccagaagac ttttacaaaa
     7381 ctgattacac cctactatgc ggcaatttgc catccattga gtggagtgaa gcgttgttat
     7441 tgaaaaataa tactcccggc aaagttattt tgcttgatgt gagggaaaca cacgaacagc
     7501 cgaagttgga tggaatagaa acaatgaata ttccgctgca gtcactttct gaaaatattg
     7561 agccattaaa tacagcagaa aatattttag ttttttgtca gtcgggaata agaagcagaa
     7621 aagctgttgc agtgttacgc agcaaattcc tacagaaaaa tatttattct gttgccggag
     7681 gaatttcaaa acttaattca accatcaacg cttagcaaac atggaacgta aaccaaaaca
     7741 gatttttata cagggggcca tttcttcctc aaaaattgca gactccattc aaaagcacag
     7801 cagtaaaaca gatattggcg cacacagtat ttttttgggg caggtaagaa ccgatgaaaa
     7861 gccggaagga atcattcagg cgattgaata cacaacttac gaagagatgg ctttacagaa
     7921 agcatttgag atacgcgaag atatttttgc aaaatacccg ctcacctgtt tacacatcta
     7981 tcatagtttg ggtgttgtaa aaaccggaga aatctgtttg tttgttttta cttcttcaaa
     8041 aaaccggaaa gcagcaacag aagcttgcaa tgagatggtg gaacgaatca aaaacgaatt
     8101 gcccgtttgg gggaaagaaa ttattgatga gaaaaaatct ttgtggaaag aaaacagtta
     8161 aagaagtatg ataaacatta cgcataaaaa cagcacgcac cgcactgcca tagcacaggc
     8221 aattgtgaaa gtgagtaaag aggaaacgat aactgccgtt aaagaaaata aagtgcccaa
     8281 aggcaatgtg tttgaaatgt cgaaaactgc gggattgttt gccgccaaac gcacatccga
     8341 tatgattccc gattgccatc ctcttcccat tgaatatgca cacatcacac atgagataaa
     8401 cgggttggaa attttaatcc gcgtggaaat tcaaaccatt tacaaaaccg gagtagaagt
     8461 agaagccatg catgctgctt ctgttgttgc acttaccatt tatgatatgc ttaaacctat
     8521 tgataaaaat atttccattg aacaaataaa actgattgaa aagaaaggcg ggaaaagcga
     8581 tttcattaaa aaggaaataa gcggaataaa ggcagccgtt atagtttgct ccgacagtat
     8641 ttcaaaaggc acaaaagaag acaaggcggg taaagcaatt attgaaaaac taaaatcttg
     8701 taaagttgat attgccgatt acattatcat tcctgacgaa aaaaatatta ttcaggaaaa
     8761 agtaagagca tattgttctc agcagattca actggtaatt cttacgggcg gcacaggatt
     8821 atcgccacgc gatattactc cggaagcatt agacagcgtt atcgaaaaac ggatacccgg
     8881 cattgaagaa gccatccgca cgtatggtca ggaacgaaca ccatactcta tgctatcgcg
     8941 cagtattgca ggattagtag gcaacaccct tgtattggct ttgcccggct ccacacgcgg
     9001 tgctgccgaa agtatggaag ccgttttccc tcacatactc catattttca gagtgatgaa
     9061 agatgcgagg catgattgag aaaatatacc ttaaagaaaa ctcttgtaat caagtctgaa
     9121 ttaccccaac attatatggt ttagtaatag gattgtgatt tgccatttca atgcccatgc
     9181 taatcatatt acgagtctct aatgggtcaa taattgcatc aacccaaagt cttgatgctg
     9241 cataatatgg tgatgtctgt gagttgtatc tgtcagtaat tgtttttagt tgtgcttttt
     9301 cgtcttcttc agaaatttct ttaccctgtg ctttaagagc agccacctga atttgcaata
     9361 atgtttttgc tgcttgtgaa ccacccatca ctgctatttg tcctgtcggc catgcagcaa
     9421 ttaatcttgg atcgtaggct ttgccacaca ttgcatagtt gcctgcacca taactgttac
     9481 caatgatgat ggtgaattta ggcacgacac tgttagccat agcatttacc aatttagctc
     9541 catctttgat tatgccacca tgttcactac gactaccaac cataaagcct gttacatctt
     9601 gcagaaacac taagggtatt cttcgctgat tgcagttcat gataaacctt gctgccttat
     9661 ctgcactgtc gctgtagata acaccaccaa actgcatttc gcctttttta cttttcacaa
     9721 ccttgcgctg attggcaaca attcctacag cccatccgtc aatacgtgct gttccgcata
     9781 aaatactttg tccgtacttt tctttgtatt catcaaactc actgttatca actaatcggt
     9841 tgataacttc atgcatatcg tattgcttgg aattttctgc cggaattatt ccataaattt
     9901 cttttggatt tgctgcaggt tcctttgact ctgctctctc aaaacccgct ttgtcaaatg
     9961 ttccaatttt agaaaacaaa cgtttaatat agtcaagaca agatgcatca tcaggaaatt
    10021 tattatctgt aacacctgaa atttcacaat gagtagtagc tccacctaaa gtttcattat
    10081 ctatatcttc accaatagca gactttacca aatagcttcc tgcaagaaac acagaacctg
    10141 ttttgtcaac tattaaagct tcatcactca taataggtaa ataggctcct cctgcaacac
    10201 aacttcccat aattgctgca atctgtaata ttcccattga tgacataact gcattgtttc
    10261 tgaatatgcg accgaaatgc tctttgtccg gaaatatttc atcttgcata ggaagaaata
    10321 cacctgcact gtcaacaaga taaacaatag gtaacttatt ttccatgctg atttcctgtg
    10381 cacgtaaatt cttttttgct gtcatcggaa accatgcacc tgcctttaca gtagcatcat
    10441 tagctacaac gatacattgt tttcctgaaa tatatccaat cacaataaca acacctgcag
    10501 aaggacaacc accttgttcg gcatacatgc catctgctgc aaaagcacct acttcaaatt
    10561 gtggtttatc ggtatccaac aaatacgcaa ttcgttcacg tgccagcatt ttgttttttt
    10621 ctttttgctt agctgcattt ttctttccac caccttcata aattttattt aatcgctctg
    10681 ctaaacgaat caaatgtgat ttataagcat cttcattgat attaaattca agattaactt
    10741 tctctgccat aattagtatt caatagcctg caaaaataac ttaaaaaagt caactttact
    10801 gaatgtactt taggcaagtc tatgactttt gtttattgtt cttatctgcc ttcttagtat
    10861 tgtaattcca tggacagtgg cggcaaccgc tttgacaaca atatcctctt tttagatgat
    10921 atttctcagt aaatacaata tacccttcag gagtcaggta gtattcatct ttttcaaact
    10981 tgtctgtaaa agccatttcg tttgcaaaat tcaacaatat acagattgtt ttcaaataat
    11041 attttatcgt atcgtattga gtgatgaaat taaggatgta tatactcaaa gcaattcatg
    11101 tataactctt tagatttgca aaaatgaata gtccaattat tgatgaagta aatctcaacg
    11161 actttcagtt ggcacataaa ggagtgagtt tacatgtact cagactcgat ttggatgatg
    11221 tcataacagg aggcaataag tcttttaagt taaaatacaa tcttcaagca gcaaaagaaa
    11281 aggggaggag cataatagtt acattcggtg gtgctttcag caatcatatt gcggcaactg
    11341 cacgtacctg caaattacaa aacttaaaag ctgtgggtat tatcagaggt gaaattgtta
    11401 cgccaatgaa taacacttta agtcgtgctg ttaatgatgg aatgacgtta atacctgtca
    11461 gtcgtcaatg gtatagacaa attcatgata aagaatttta taaaagtaat ttagtaaagt
    11521 gtttactaat tgcaaacaat ataaatcctg atgcttgtta tgtagttcct gagggaggaa
    11581 ataatgccga aggctttaaa ggttgtttgg aaataatgaa taatataaat gctgattatg
    11641 atacagttgt atgtgctgtt ggaacaggaa ctactcttgc aggaattgct gcatcactac
    11701 cccaaaataa aaacgtacat ggtattgccg tagttctggc tgccgatact ctgactaaaa
    11761 acatacaatt acacttagct gaaagtaatt caaatgcaaa gtatattgtt caccaacaat
    11821 actcctttgg tggatttggt aaaagcaatg cagaacttaa aacattttgt gagttattta
    11881 cacaaaaaac taatattctt attgagcctg tttacaccgg taaattgttt tatgcaatac
    11941 ttgatatgct tgcaaaagat aagtttaatc cgggaacaaa aattcttgct attcacaccg
    12001 gtggttgtca gtatttgatt gataaataaa aaaaggcaat ccgaagactg ccttttcaaa
    12061 atatattaga ctttataaaa cttaatccaa agttattttt ttggtaatca cgtcagagtt
    12121 gtaagttatt tttactaata caacaccttt gttcagtgat agtgatgcct tttctattcg
    12181 ctgattaaat gcatttggat ttttctcaga tgctaaaaga ctgcctttaa tatcataaac
    12241 ttcaatagaa cgcattggct ctttgctggt agaaaccaca gtaaaatagc tttttgaagg
    12301 actattatag attgttacat ctgaactgtt tgtagaaact tctttaacag atgttggcag
    12361 gttacggcag aaaacgattc tgggatttac gtagttcata atggtatcaa tgtaagccaa
    12421 agcataagtc ttgctcatag ttgggtttgt taacatagaa ttaccataac aagtatctcc
    12481 gcgtgttgac tgataaccca ttgccatagc aagattacgt gttgattgta aatcgtacca
    12541 ttcccaagga ccggcatgtc caagttgtgg gtttaccgga ttaatacctt gctgaagtgg
    12601 gaacaatcct tcattgccat tattaatact gttagcacga agtgtataag gatccgtaaa
    12661 ccctgcatta gcaaaacatg cattatttcc taattgatta gcccatttaa taatttcacc
    12721 tgaacctatt acatcaacaa cagcttgtgg tggagtagtg ggtacataaa caattccaac
    12781 accgtaagga gcaaatggat cgtcaacaca atgaattcca atcataggag catcaccggc
    12841 agcaatccat gaactatcac ccatggcacc acctaaattc actacaaact gaacagcatt
    12901 tgaatatccg ggattatttt cattgttata gggtgccaca ccaccaatgc cgtcaaaatc
    12961 acccaatgca gtttgattta cataaggctg tccggcataa atgccatagg ttggtgcatc
    13021 agtgctgctg ataaacttag gcagcgtaat ttcagacgga ctgtttagca ctgcatagtt
    13081 caatgcaata tatccgcctg ttccctgacc aacgagataa aaattattca catcaatttt
    13141 ataaacatta ttgtttgttg cagcatcttt tctgaaatat cgcacacatg actttgcatc
    13201 ttgtattgaa cgataaactg cattcagcaa ggtacctcta cgaatgtcaa gtccattgtt
    13261 tggattcgta tttgtaggat cccatcctaa gcggtaatcc atatttgcaa caacatagcc
    13321 tctgcgagca aactgcttgc aaatttctac tgctgcactg tcatttcttg atccggttgc
    13381 atttccattc acaataatag gaatataact tcctgtatgt aaataaataa tcaacggtct
    13441 ttcagtcatt gggtcagcag cacctgcagg ctcataaata tccattttta aatcttttaa
    13501 aactggtgtt cctgtaagga cttcgtagtt ctgaccataa actacattgg gggtaacatt
    13561 aacagaaggg aatacttcag ttaaaaatcg ttgtgcctgt gcagtatatg caaacatgct
    13621 gaacatgccc aatactgcta atttttgtaa atgttttctc atcttaatat ggttttatgg
    13681 acgtaaaaat aataacaatc ttcaaataaa aagcaattac tgaaaactta atctatcagg
    13741 tcaataaatt ctgataaaat cattgctgtt gcaccccaaa taaccaggtt gccaattctg
    13801 aaagcaggag ctgttattaa gcctcttgta gtctgatgtt gcagtccttg tataattgtt
    13861 ttatttttaa tcaaactatg caatgggatt tcaaaaattt ctttcacttc attttgattt
    13921 gctaagaaaa ctgatgtttg aatctgagtg gcaataaaag gatataccat gtaattgctc
    13981 ggtggaatat atattcttga aagctgacct atgatgttga tgctcttatc agcaattcca
    14041 atttcttctt ccagttcgcg ggttgcagtt agagtcaaac tctcatcagg ctctgccttt
    14101 ccaccaggga aagcaatttg acccgaatgc actttgccat cttctgcacg ctgaatcatt
    14161 acaagatgcg gaatgttatt tttattattg acaagcaaca taacactgcc tatgcgataa
    14221 ttcccttcag gctcataaac aggacggtta acagatgcta attttaaatg agaatttctg
    14281 ccgggcaaat ctttcagtct tccggaaata aaaacttcaa agtctgttat gttatcaagc
    14341 ggtgacaact ctttctcctt ttttgcgttc accaatctgc tgacgccaca tggcataata
    14401 aagacctttc tcttcgagaa gcgtttcatg tctgccttct tcaatcttat gtcctttttc
    14461 caatacaaaa atatggtcag catgcatgat ggttgaaagc ctgtgtgcaa tcaatatggt
    14521 aatgagattt ttatttttgt tgatagattt tatagtttct gtaatctctt cttcagtaat
    14581 ggaatcaagt gcagaagtag cctcatccaa aaccaacaga ttaggattgc gcagcaaagc
    14641 tcttgcaata gaaagtcttt gcttttcacc tcctgacact ttcacaccgc cttcgccaat
    14701 aactgtgttc aggccatttt ctgctcttgc caaaagagag tggcacgatg ccatattcaa
    14761 tgcattcaat acttgatcat catgggcatg tggatttacg aataaaagat tttctctgat
    14821 agttcctgca aacaattgag tatcctgtgt cacaaaacca atctgctctc tcaactcatt
    14881 cagattaatg ttgttggcat caatcccatt gtaaagaatt tttccctctg ccggatgata
    14941 caagcccact agtaatttta ctaaagtact tttaccactg cccgaaggac caacaaaggc
    15001 aattgtctcg ccacgtctgg ctttaaaact gatattgtta acagcattgc catgtgcgga
    15061 tatatgttta aaagaaacac tactaaattc aaatgtttca attgcattca actgaagagg
    15121 tgattgtggt ttgtattcaa gtggcatttc cattatctca ctaaaattat tcagtgaagc
    15181 ctgtgcttcg cggtaaacag caataacgtt acccaactcc tgtagcgggc cgaaaataaa
    15241 aaacgaataa aacattaatg tcatcatctt accaggtgaa atttcatcct taaaaatcag
    15301 atagtacaac aaaaaaacaa acaatgttct cagaaaattc accgttgtgc cctgtataaa
    15361 actaaggctg cggataaact tcactttctt caattccaga ttcagaattt tatctgtcac
    15421 tgtgttcagt ctgtttattt cctgatttgc cagccccaga ctcttcacta actcaatatt
    15481 tctgagcgac tctgttgttg cacctgctaa tgcagtagtc tcacccaata tttttttaga
    15541 aataacctta atctttttgc tgagcatatt gctcaccaca gccaaaaccg gcacagtaag
    15601 cagaaaaaca ggtgcaatgc tccaatgtac atttatagca taagccatca caaaaattat
    15661 tccaaccaca ctctgaaaaa gaatactgat aaatgcattg ataaacttct cgctgtcggc
    15721 acgcaccttt tgtaacttac ctagggtttc accgctgcgc tgatcctcaa aaacctgata
    15781 gggcaattcc agcgaatgtt taatgccatc gctatacatc tctgcaccgg tgcgttgcac
    15841 aatcacattt acaaaataat cctgaaaatt ttttgcaata cgcgacacca aagctactcc
    15901 cattgaagct aaaaggaacc aaataatatc ctgaaaaaat ttaggaaaat cattactgta
    15961 gttcgataca tgcatagcaa atcgatccag cattttaccc gcaatcaacg gatccaataa
    16021 agaaaaacat tgattaacag ccgccagcaa caaagctaat aataccaact tccagtggcg
    16081 ttttaaataa gttactaata ttttcaaact tcttgatttg ttaggagttt caaaaatagt
    16141 ggtttaggaa gtaggaaatg aatcagtagt ttaatttgta ttaatattga gtgggagggt
    16201 taatatttag gtaaggtttt attataaaaa tgtgtcaatt ttaaaaagtc aacttataat
    16261 tcttttaaaa ataaaaattc attactggct tattttatct ttttattttt atttttgaat
    16321 tgaaaaatag ttttaattct gatatgtatc agaacaaaac aatatgctcg atagcgattg
    16381 gacttactat ctcattgaca attctatgca acagctaatt gatacataga aaataattaa
    16441 aagacacagt ttattttata tgcaaaaaat cgttaaacta tatgaatgga caacctcctt
    16501 acctattatt acatgtttac tatatttttc aggtttatta gataacaata ttgttttgca
    16561 aatattggca ggaatattac tgctgttttg tgtaatgtca gctgtacatc attcagaaat
    16621 cgttgctcat cgtgtgggcg aaccttacgg aactattatc ctggcagtat ccattacaat
    16681 tattgaagtt gctataataa tttctttaat gatgaccggt ggtaaagaat atgcttcatt
    16741 cgccagagac actgtttatg ctgctgttat gttgattctt aatggaatta taggactttc
    16801 attgtttctt ggcggaagaa aatatcatac acaaacattt tctcctcatt cagtaaagat
    16861 tgcactggta gcacttgtgt ctattgtaac attcacaatg atattaccca cctttacaag
    16921 tagtattatt ggcccatatt acacagaacg acaattgtta tttgaaataa ttgcgtgctt
    16981 aattatttat atagctttta tagcagctca aacaattaga tatagggaat attttatcac
    17041 tgaagctaat cccgatgtag aagtcactca caataatatc tctaattcct catttattat
    17101 tagtttaatt tttctaatga tttgtcttgt tatagttgtg ttacttgcaa aatcactttc
    17161 caatcccata gaaaatttat taacttctta tggattgcca cgtagtttag tcggaattgt
    17221 tattgcagct gtaattttat tgcctgaagg aattgcagcc gtttcatcgg ccagaaaaaa
    17281 taaattacaa aacagtttga acttagcttt aggttcggca cttgcaagta taggattgac
    17341 aattcctgct gtttctcttg caagctacat ttttgacctg ccgcttattt taggtctcga
    17401 attattgcct ataatattat tagccatttc tatttttact gtaatgcttt cactcattgg
    17461 aggacgtagt aatattgttt atggtgtcgt tttgttagtg aatttatttg cgtacgtatt
    17521 tttaacaatt aatccataac tgtaatttta acacaatatt ttactatagt aagttggttt
    17581 taatatctga atcagatttt tttatcctat caaagtatca atgattttac attggtggga
    17641 ctctgtggaa tagcaagcaa tataacttac aaaacattaa aaataatcta atcatttcat
    17701 tgccttatca ttttttatta ctattgtaaa tggatagttt taattccatt cgacataata
    17761 aagttagtta tctttggtaa ctgtcaataa tagagattta gctaaatgga gaccttaaag
    17821 aaaagaataa acaataatgt taaccaaaca tacacatgaa aaagcttcta cttcttctat
    17881 ttgtaacttc acagattaca ttttgcaaag cacaacaatt ttcttttcaa atgttttttt
    17941 cagatgccat tggtaataaa gacaccatta ctttgggata tgatttaact gcaactgata
    18001 gtattgatac tgcatttggt gaaacaaata tcatttcagt tcctttagac acaaatctgg
    18061 atgttcgcat tacaaatgca tggtttactt atgcagacgc tgtttacttc catttaaaaa
    18121 agcaaattgt tttctatgat tgttctctac ataatttaaa tattcaacca attaatattg
    18181 ttactcaaca ctggccggtt acaatgacat ggaataatag tatattcaat gactcatgta
    18241 ataatggaag tgtttttaca ggtattaatc ctgctggttg gtgggacaca ggctgcccaa
    18301 gcggcttatt acaacaggtt tttctaggtg atgactcaat caccttcaat tctaactttg
    18361 atttaggttg gttcggttat atcaattcaa atggtgattc aattccggtt ttttggcaag
    18421 catttggcga ttcaacaatt cttactaccg gtattaatga acttccgcaa caatgtattt
    18481 cagtaaaagt atttccgaat cccggttcta cttacatcac tgttcaagca aacaattcat
    18541 tcggacagtt aagcctgatt tcaatcttaa attcttatgg acaaattgta ctcaaaacac
    18601 aacagataag taggattgat ataagtgagc tccccgatgg aatttatttt attaaaacag
    18661 aaaatactaa gggagaattt acaacaagta aatttttaaa actctgaggt aataaatata
    18721 ttcaacttca acagatcata tttcccccaa aattcatatt atcttttcta ctttgaaaga
    18781 ttgaattact aaattcaatt tcgagtattc agcaagtatt cagaatatta cttatcctat
    18841 caataaatag aaaaagtatt attaggcaat tcaaaacact tactgaaaaa ggatgtacca
    18901 agattgcgca agaatacatt tccgaacaaa acattctact ttcttttact attcaatcct
    18961 ttcaagtcct gtttgataaa caagagtata agaaactaca ctatatgtat tatttccatt
    19021 ttgcaatttg cccaaactta actgtacgtg aattccaaat cttactctgt attgggttgt
    19081 aattccacga agataccata gctacaattt ttaaatctga taatttatag ccgacaaccg
    19141 ggaacgaata gctcattacg gtttccgttg aagtaaacgt gatatcatca ccaagtgcgt
    19201 tagttggcat taatcgaaca acttgctggt gaaaaaaatt tcccacactg cctaattggt
    19261 tttgtgcaag cacattatct tctgttacaa aaattgttaa acgcaaatta tcgttaatgt
    19321 gagaattatg acctatatgt acctccactc tgccggaatc attttcaagc tttgaaatta
    19381 aagcaatacc caaatcatgt gtttctgcca gacgcgatgt tacattgctt tgccactttg
    19441 tgtaatctac ataatatgta ccagtactag gttgaacaac cctacccaca gttgcacaag
    19501 gaaaaccgaa catcgcttgc caataatgga atcttacagt gtcatattta actatttcaa
    19561 gccaatcaac atcgtgtatg gaaaccacat ccactttacc gccattaatg gctgaatctt
    19621 taatagattg aagaatatga gccccattaa cacaacctgg acaccattcg cccgtcagtt
    19681 cttcaatcaa aactctttgt tcaaaaacag aaggcatagt accgagtgag tttgtaatac
    19741 tagtcgaatc tggcacattc ggactaactt catcagtatt atcattttta cggcatgcgt
    19801 taaataatag aaaaagtagt atagcagtta aaaaaataaa ttgtttcttc atgacatgat
    19861 atttattcag taagtgttaa aaatgcatta aaagtttcac attttcaaaa ataaattttc
    19921 ccattatcct catattcgct tcagatcccc acgacaaaac agagtttgaa gctttgggga
    19981 ggtaactaac attcaacaac tttctaaact ccaaccctta ctttcacaat cttatagcta
    20041 ttagcatttg acttgtgctt tttcaaccca aaattctcaa cccatttttc tcttgcgcta
    20101 aaagccgtaa aaatcaaaac cgccattatg cttagcacaa cagcggttta tattttatta
    20161 agtgtattta atttaactaa tcaacaaatc accaattatc aaatcaaaca actttctaaa
    20221 ctttcaaacc ttctaaactt tccaacttag tttagtagcg ataataatca ggcttataag
    20281 gtcctgcaac tggaacgcca atataatctg cctggctttg cgttaatgtt tccaattcaa
    20341 caccaatttt acttaggtgc agacgtgcaa ctttctcatc aagatgcttt ggaagtgtgt
    20401 aaactttgtt ctcatattta tcggtatgat tccacaactc aagctgtgcc agcgtctgat
    20461 tggtgaacga attactcatc acaaatgatg ggtgaccggt agcacaacca aggtttacca
    20521 agcgaccttc tgcaagtaca ataatgtctt taccatcaag cgtatattta tcaacttgtg
    20581 gcttgatagt atctttagaa ttaccataat ttttattcag ccatgccata tcaatctcat
    20641 tatcgaagtg accgatatta caaacaactg cattatgctt cattgcttta aaatgtttgt
    20701 caacaataat gttatgattt cctgttgcag taacaataat gtctgcttca ggaatagcat
    20761 tgtccatcgt ttttacttca taaccttcca ttgcagcctg aagtgcgcaa attggatcaa
    20821 tctcagttac aattacacga acaccggcac tgcttaacga ttctgcagaa ccttttccaa
    20881 catcaccata acctgcaact actgcaactt ttccggcaag catcaggtca gtagctctgc
    20941 gaattgcatc ttgcaacgat tcgcgacagc catatttatt atcaaacttc gacttggtaa
    21001 ctgagtcgtt aatgttaatg gcaggaatag gtaatgtgcc tttttgcata cgctcataca
    21061 agcggtgaac acctgtagta gtttcttcgc ttaatccttt aatatctttt atcagttcag
    21121 gaaatttgt
//
LOCUS       DAOOOL010000034        20411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_200_length_20411_cov_437.885439, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000034 DAOOOL010000000
VERSION     DAOOOL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_20411_cov_437.885439"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1453)
                     /locus_tag="PKU82_04480"
     CDS             complement(47..1453)
                     /locus_tag="PKU82_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOZ82164.1"
                     /translation="MALKKSIQSFSNVESIEIDKIVHNGNYRKHIDEEKIIELAESMK
                     SVQLLHPIMVRDTGKGKYHLVFGERRLLAAKSLGWEKIDSRIIQNVKPDKLVEIQLIE
                     NVQREQPHPIDESETFVQLTKITSVEQIASTIGVSEQYVYDRIHLKSLNAEIKRLFKD
                     SHITLHHCLQFAKLQNAKQLELLERIKYNVPYNEKEGEHRFAYRSVKEMRRIIEKEFV
                     CLLTKAPFDIEDKRLNKSAGSCINCMKRSGANRTLFGDVQSDDLCFDTKCFWKKTSAK
                     LTTLEKQLVDAGNTVIRIDTSLIPDENEHENLVSLTEVHQIPENEVDNIDSKLFGIIF
                     SSDNIQLLGTVIKLYEKVPEVNEVETIIHNEPVKATVTPSQKRMENVKIKNGRISLKN
                     KLKEEVGFMLGAKELDKMPVSILKMLFYNIIAFNEVSELLENLKWKYDGMPVNSPEII
                     ELDIMKLVNENVNNQSYF"
     gene            complement(1419..2000)
                     /locus_tag="PKU82_04485"
     CDS             complement(1419..2000)
                     /locus_tag="PKU82_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82165.1"
                     /translation="MTTDVTAYDNCISTYSGLRFDFINPRPDMVNVLDICKGLANKPH
                     FSGQTPEFFSIAEHCILVESLLPYDAEPEERLCALIHDGSEAYLPDMIKPIKLLLPLF
                     KDIEDGVQSCIFDYFGLDERYMKRIKKYDIMAQQIEYNVFYFNHATLANNYHICYLKP
                     KDAYLAYIKRLFYILQTIKYDSNGTKKKYSIIQ"
     gene            complement(1997..2830)
                     /locus_tag="PKU82_04490"
     CDS             complement(1997..2830)
                     /locus_tag="PKU82_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ82166.1"
                     /translation="MSYRVPTPNTNFDLETTGTNVLTDRIVQIALVYTNSIGMRDTRN
                     ILINPGIPIPVDATDVHGITDEMVKDASTFPEIANDIFQYMVDVTLIGFNIKNFDIPL
                     LKEEFKRCGIDWDVPKDIIDVFQMDKALYRRTLNNVYKQHTGLDIDGAHDALNDVYAV
                     DVILQSMVKYNNTEVQNFLTTSKDQVDIAGKFLLDEVGNIVFGFGKFQGNQVSKHIQY
                     VQWMNQQEFFTADTKDFCKKILAGAFRVEQLTNKPAEVVDEAKEKILRDLENAEVDPF
                     A"
     gene            complement(2970..3224)
                     /locus_tag="PKU82_04495"
     CDS             complement(2970..3224)
                     /locus_tag="PKU82_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82167.1"
                     /translation="MKLNLSEHQILVADCVTIAEFVKQLRASTGNEKINASTIHYHLK
                     NTDNLDYVEVNNVFLIVVNDKAKSFNPGSYYKGRISKMDL"
     gene            complement(3221..3643)
                     /locus_tag="PKU82_04500"
     CDS             complement(3221..3643)
                     /locus_tag="PKU82_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82168.1"
                     /translation="MLILLVAKASLGISQINPPVKINDKFCFDKQSTLELIKSDIKAK
                     SYDSLLLTSLTYEKLVVEKNNINDSIILDQATQIDNLSLIASEKDKQIVLLKNENKAL
                     KKENRKRRRKLFFSNFTIVTGVAIGVTTYAVIKAKSKK"
     gene            complement(3576..3968)
                     /locus_tag="PKU82_04505"
     CDS             complement(3576..3968)
                     /locus_tag="PKU82_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82169.1"
                     /translation="MKTEIKKEIFAFVKRDWIYIAIFAATLIITGIVFRIQSSADNTK
                     ELSGIIKAIYAEKQMVIDSLTKQNKLLQSKIDTLTWRKNVITKEIVYIKQKQNGIIKT
                     TSQKIADVDTFGSESIIRYFSNKSSGKN"
     gene            complement(3970..4200)
                     /locus_tag="PKU82_04510"
     CDS             complement(3970..4200)
                     /locus_tag="PKU82_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82170.1"
                     /translation="MFIVALIFSWFPSVFISLRFLLAKVYANLQKKNEAENNLESLLI
                     INTVMVELETLSRYFLWYGITATVLILIIKYV"
     gene            complement(4207..4827)
                     /locus_tag="PKU82_04515"
     CDS             complement(4207..4827)
                     /locus_tag="PKU82_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82171.1"
                     /translation="MAPAGTELDEVISKLVENAKENYSKEIVFDFAKEPFPNYIIPSD
                     VTFDQIREYCYPDITLEFKVVGNEVTEYHSNQLKEIKYTFTPEEKLELADRLCEFEKM
                     REEQEEMKKAETKRLADIVKNTETNISIIASKYREGYEWRNVDTILAFDFANKQRIYM
                     EPGTHVVLHVEDLKAEDYQLQLNLDNMTTPVEEKTEEQVTDEDIFK"
     gene            complement(4882..5193)
                     /locus_tag="PKU82_04520"
     CDS             complement(4882..5193)
                     /locus_tag="PKU82_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82172.1"
                     /translation="MNILLRMHWTKRAKMLEQIQWKIRSQTVSRHPGKVRIVFHRFAV
                     KLMDWDNYAGAFKFIGDALHGNKIIVDDKPAIVVEFIPKQTQVDTLKQQKILVTIEDI
                     I"
     gene            complement(5382..6032)
                     /locus_tag="PKU82_04525"
     CDS             complement(5382..6032)
                     /locus_tag="PKU82_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HOZ82173.1"
                     /translation="METERKELLKMCDGKKYNVIYADPAWRFSQGINRRKDIREKFGT
                     NEELSIQYPTMSDKEIIELNVADYADDNCVLFVWTTDAHLEVAIKAINNWGFKYKTVA
                     FTWNKKRGFMGKWTVKQCEICLLATKGTAHKLLKSFKEKSYLEENKTEHSKKPNEFRK
                     RIERMFGDVPKLEMFARTRAEGWDVWGNETDKFEEEKGGKIFNNSFPPQSLFGDVS"
     gene            complement(6088..6288)
                     /locus_tag="PKU82_04530"
     CDS             complement(6088..6288)
                     /locus_tag="PKU82_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82174.1"
                     /translation="MIEIITEKVEMPSLSTKRRFQKICINNKWEVIDTHNENKTRYKG
                     AYEDVMIACHNLNKKFYRDGVV"
     gene            complement(6453..7268)
                     /locus_tag="PKU82_04535"
     CDS             complement(6453..7268)
                     /locus_tag="PKU82_04535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOZ82175.1"
                     /translation="MIDRITEQPFCQTRVMCRAVDNLELLLSQPNESVDLIYCDILYG
                     TGRNFGDYQDLKPIRSEIESHYLPRLKEMHRVLKSTGSIYLQMDTKINHWIRCLMDEV
                     FEYGNFRNEIIWCYSGGGRKKKDFAEKHDVIYRYSKTKNYTFNEVRIPYTIGGGTDTA
                     LKSGKIMDKNINGKVHNWKPNEEGKIPEDWWCIPFINPMSNERIGYSTQKPKALVERI
                     IKASSNEGDTVADYYLGSGTTAVVCKELNRNFIGCDINPKAIEITNARLDAVS"
     gene            complement(7411..7821)
                     /locus_tag="PKU82_04540"
     CDS             complement(7411..7821)
                     /locus_tag="PKU82_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82176.1"
                     /translation="MNNLNDLSKQIYEGNKLRGFDVSKENIGQTLMLVVSELAEALEA
                     DRKDKRKMLNAFEQDLGYAKLSISDFETENENCDWLKNRFETTVKDTFEDEIADSFIR
                     LFDLCGGLGIDIEKHIDLKLRYNSTRTFKHGKRY"
     gene            complement(7998..8231)
                     /locus_tag="PKU82_04545"
     CDS             complement(7998..8231)
                     /locus_tag="PKU82_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82177.1"
                     /translation="MEDQKYYQKQREAILNDSQRKELFKALFELYANAMPIALKKDDG
                     TVICSYSDKVEELAEKIREQIRLRDNQVFEAHS"
     gene            complement(8291..9076)
                     /locus_tag="PKU82_04550"
     CDS             complement(8291..9076)
                     /locus_tag="PKU82_04550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOZ82178.1"
                     /translation="MMNTVESPTCTKPMLGVVLLEDCLTTMGKLTDNSVDLIITSPPY
                     NKAGYEGFIRKRHEKDSWSKGRNIEYGGEADNDFMIEAEYQEQQIKVLNEMHRILKPN
                     GSVFYNHKVRVAQHKASHPIEWILKSNFTFRQQIIWDRKNSPAVAPIRYLPNTELIFW
                     LTKTPCQPNFERAKAPLFVGEVWQFSAKPNPLHPAPFPEELPTNIMMCIKDKTEDFVV
                     YDPYAGTGTTLKVARSFGLKYFGSEINSNYCRLANETLNGTLF"
     gene            complement(9157..9324)
                     /locus_tag="PKU82_04555"
     CDS             complement(9157..9324)
                     /locus_tag="PKU82_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82179.1"
                     /translation="MEHTNKIAVKIIIEEFDDYKNNPENQWHYDREYKMIVKALEEQE
                     KLNKHDSMIIL"
     gene            complement(9330..10052)
                     /locus_tag="PKU82_04560"
     CDS             complement(9330..10052)
                     /locus_tag="PKU82_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82180.1"
                     /translation="MQNLKHLLTEIRTTPKEKNLTNREQMQFDAFLEVFKSVAGHYGR
                     KGVFSIDDENREQIKYLLLYFSNNRAFNEITPNNVMKYKPDLEKGLILAGNVGSGKTN
                     LLRIFQEMRIPSNYFRIAYCDVITDDYRRLGMQSLFKYYNSNLERIQHDTSESIFLFD
                     DLGFEKKFKDYGTEVNCLMDIIIKRERLYNDYGTRSLFTTNLMLSEIESYYDNRTLSR
                     LCGMCNVIYLGNKSDSIDRRIN"
     gene            complement(9988..11151)
                     /locus_tag="PKU82_04565"
     CDS             complement(9988..11151)
                     /locus_tag="PKU82_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82181.1"
                     /translation="MSGYIKLSRKYFNNFLWNEKRKFSKAEAWLDLIQLVRYDATSNG
                     KKIVNGKLIEWKRGEFPASSRYLNERWNWSGPSQVKRFLDLLKNEDMIATRTEQGETV
                     ISLCNYDSYNTDWNDVGTVVRTVVEHQQNGDKTVEEQKQNETVIQSKKDNTVKNDEKG
                     NIPSVDGFNPPAEKILKEEYRKEFLKVMKFIFEQPILQKIPKTLTVDEYEKLREKFTF
                     EQIEEMLIAMANHKATNKSQTIYLTLLTYLKKNFSQGEVKELEPIFEKMYRDFIVKQE
                     PDIHAVKLTDFDRRSMRTIIGYLLENNVNANKKRNDNRDTSITEFTDKDGAVISWRYI
                     LTNWNKLDNFMQKRVKLTEIDNDLMKILTDLKNAKLKTPADGNKDNSQRKEFN"
     gene            complement(11155..11439)
                     /locus_tag="PKU82_04570"
     CDS             complement(11155..11439)
                     /locus_tag="PKU82_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82182.1"
                     /translation="MSRSVRQHRSEWFYYRLIASWTGHTAYEVYEIMTKLFLLTVDEE
                     GNLGIIRPTSLTTVEHNEYLEQIRCFMAGFEFYLPEPNEIPTEDYIIKII"
     gene            complement(11411..12169)
                     /locus_tag="PKU82_04575"
     CDS             complement(11411..12169)
                     /locus_tag="PKU82_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82183.1"
                     /translation="MPQKRKLKNQQLNYSKMKTLKPKYKITPSKLESFRYLIEEHYGT
                     TEDFIAEIKGTKEWSRKMNFGSAFHAVIEFGPEKYYDFDSGKYIVKESDFPEAVVMEQ
                     EQIDVAVRFRNNHSRMVSECKHSFELDMGAFIAVFSLKVDSLEGDIVNENKTTDKAVS
                     FEKYYKSIQWKCYILATGVQKVVYNVFKYKGNEKKGYEVDEPVDFTFFPYENLKNDVY
                     SLCREYIDFCTRHDLLDYVTVKPKANVEKCSTAS"
     gene            complement(12096..13442)
                     /locus_tag="PKU82_04580"
     CDS             complement(12096..13442)
                     /locus_tag="PKU82_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ82184.1"
                     /translation="MEQIVQEQQWNIVEETDALKIASILTVIFGDPGMGKTSLSMQSE
                     ESILLDFDDGLKRSTKRKRAVQMRTFAGALDFIRSEKFKELKPRTVILDTGGTLLDNY
                     IAHYVIENETANKKKGGGLSLQGYGALKEVFSQFINELKRMQIDIVIVCHTEQYKDGE
                     NLRFRPKMTGGSYDILMQVADLVGYMESKNEKRTLNFNPTDRHIGKNAPNFANFIIPH
                     AETIEFDNFLGRIIADAKEKMSKLDEEQQKALLLLDEKRKEVDEADTFEKLVALTETF
                     ADLKPAFQIQLSKLVEDKKTEFIKVMISGLTEPLQLDVMLNEIKTKYGDSNFYKRLLN
                     DRKTGLGFEFDKDNGVFVKPEVKDEPGAEVKEEDKQPVLNAVTEVPAEQTNAPAEEVK
                     EEDKQPVLNAVTEVPAEQTSAPAEEVKEEVKKSASKKKAEEPTTELFKNENAEAKV"
     gene            complement(13533..15563)
                     /locus_tag="PKU82_04585"
     CDS             complement(13533..15563)
                     /locus_tag="PKU82_04585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ82185.1"
                     /translation="MQDFTELYNGLNAFRELFKQRILPETEVSISAKFLRGIAQKDYK
                     TWTIDEAIRLHNIIKPYGLKLKRVGFDISKIPVIQIQLSVVNRDNVVQPEQRRLIGTD
                     GNFFIITFPYRKELYDAVKNIPRARFMYDTKRWIVPLEFAKEVTHFANGYEFQIGERA
                     MNLINLQYESGENMELSYMTEYIELNIPLKKKLYPYQTVGGDYVRRNKRVVIADQMRL
                     GKTMQSIAGILVTNSFPCIVIVPKSLRLNWQDEWHDWTDRKAIVLNHKNVKKLPELIR
                     QGMADVVITNYDGVQSFLIDDLKEVNITTGPNAGTKYHNAKGNDLCNLFNSAIIDEAH
                     HCRNKQTIRYKCVREVFKGKEVRICLTGSPIVKGPKDLAALFELIGRIEEFGGYDKFI
                     KKYKKIDAKTLDDKGKQLQELQELNTKMRSLCFIRRERHQVNEQISEKFRKVIRVDLD
                     NQSEYDHAWLSLQSYMAERAFTTSQISKALQAEMLVKMGLLKQISARGKSASVKEFVS
                     ELIDNGEKVIIGCWYNDTVQYFKDAFREYGAVTICGRIDGHDMKPEEIHENKKKFQTD
                     DNTKVIIITYGKGGEGHTLSKASSLVMVELGWTYKDQAQMEDRGIAVNKTEDFNVYVF
                     LGKDTLDEDTYEIIDKRRVIEKEAVGGSEHIENSEESVISAMKDKILKKISK"
     gene            complement(15754..16305)
                     /locus_tag="PKU82_04590"
     CDS             complement(15754..16305)
                     /locus_tag="PKU82_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82186.1"
                     /translation="MTNSERYTVIERIYANGGIFNIDVTTGPVLTSEGIVIDDDVQLF
                     LIVVAVAAGINDADTNLLSKMSAKFATLTATLPTSVYIDAKGCNSNTNPAVADAGIFY
                     LSPQIYDNGVDPPQTYNFNVLVVDFLNNHTGITRLTSGHELFPYFEEYRANLQLEVLE
                     NVIPINYIIYHEDTNEYLIVEIL"
     gene            complement(16307..18499)
                     /locus_tag="PKU82_04595"
     CDS             complement(16307..18499)
                     /locus_tag="PKU82_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82187.1"
                     /translation="MAQKILWNWQDDDSTKHLNQRTLGIVEPGLYRGFDIDLSDVSNI
                     KLVQTVTGATIVLTDSPVFTTEQRGVVVTKQGVVIHEDDVVVLPFTPNTSGNPRIDVV
                     IMEHEYVDVMDGSNVVYSVLLGTPAGTPVAPTLTSPKTQTILGYLYVPNGAEYMDAPG
                     VVYTRAVTPNFAGDGSIAHQYLLNIFLGIIGMVRNCVLDNVGNDDPVYYFSLNLNSAP
                     AGAVNKDANNFILDYGYTGGIQNENVRLRYVGPDENLGGKEVTIYTKQELRIDFGYDI
                     LPSDGDATYCYIPANSVFVLKSVSNTSPVVTDNNKWVLVNAQRASENRSNNFTASNKF
                     SGTVTENTKQITIGDDGKSSSFTATDGNILKITSMGSPSTVGATSVTGFKALPVDTII
                     KIDLSDAGITLDHVIFKNNDFVASGGNLPIYTTNSGDLVVNKGGCVWLKAEASAYRVV
                     AVQGEKKHYDGYFVLDNSAFNALSKTITVNNRNNCFSILVPTAGSLENINVVDDYGAT
                     VDVRPGTIINIRFKHIGGSIASSGGVLTAVPFLISLENTSNIRSNVSDFGYISSLGKS
                     LSIIDDQIYGESGDVITFRKTDTVWEIVSANRDAYYAHKLNDKKIDKGVTWLTADFTH
                     SSISPREASRPAMRLCKNDLGMMIVEFSFGVSADIAAGTIIGRFVGVLNGYVWSKAVP
                     VTYKQNGVGFSIGHLSFTIGPTFQDMQIIALTAIPNTYGIYQPILLIP"
     gene            complement(18501..18881)
                     /locus_tag="PKU82_04600"
     CDS             complement(18501..18881)
                     /locus_tag="PKU82_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82188.1"
                     /translation="METANNNEILQKTFEYIVGTDDIAFAIAGVFFVVIGCILSVTIR
                     ALRVGVKMPGTPNKFSSRYMFKDKIAELLLGFVLACICMRLFNDWIGAQATMGLGFVL
                     GFCNYRLANLIPKVFKFLIEDKLK"
     gene            complement(18885..19205)
                     /locus_tag="PKU82_04605"
     CDS             complement(18885..19205)
                     /locus_tag="PKU82_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82189.1"
                     /translation="MYRRKNTADVDHIIMQNMEILVNSYKDNKLLDEQIKSELRTEIA
                     EHKINLRTIRERQEMYLKNSTDALSLLANTQKELVVIKENYQKCMKEHEFARKEIETL
                     KEKS"
     gene            complement(19301..19852)
                     /locus_tag="PKU82_04610"
     CDS             complement(19301..19852)
                     /locus_tag="PKU82_04610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOZ82190.1"
                     /translation="MAIFVSAGHNPNGIKKDPGAVGNGFREADLTVEFRNLVLGSLLK
                     RGFKGIADDDSETLGQYLKRIITGNGSVVLEFHFDAAINPTATGCTSLIGSDADKNDK
                     HFGNELVEATAQILGIKNRGCISESESHRGRLGLMRERGIVALLEICFITNASDLKKY
                     QDNKFTLADKIAEIIIKYENLIN"
     gene            complement(20131..>20411)
                     /locus_tag="PKU82_04615"
     CDS             complement(20131..>20411)
                     /locus_tag="PKU82_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82191.1"
                     /translation="YEYWHKHLSSKNKYEYTGKVKVWLMKNLLVMFTDGWHKYQFVLT
                     ICLLVSGFFVGFISGKITPYYSFMLLSVYFVRTATFHLLYTSKKYRKL"
BASE COUNT         5746 a         4340 c         3088 g         7237 t
ORIGIN      
        1 ccctgctttt ggcaatacct tgttaggtgc agtgcttctc agaaattcaa aaatacgact
       61 gattattgac gttctcattg acaagtttca taatgtccaa ctctataatt tcaggactat
      121 tcaccggcat tccgtcatat ttccatttta aattttccaa caattcagat acctcattga
      181 atgcaatgat attataaaat agcattttca ggattgaaac aggcatttta tccaattctt
      241 ttgctcctaa cataaaacca acctcctctt tcagtttatt tttaagggaa atacggccat
      301 tctttatttt aacgttttcc atacgctttt gactaggcgt aacagtcgct ttaaccggtt
      361 cattatgtat tattgtttcc acttcattaa cttcaggcac tttctcataa agtttaatta
      421 ctgtacctaa caactgaata ttgtctgaac tgaagatgat accgaataac ttggaatcga
      481 tgttatctac ttcattttca ggaatttgat gtacctctgt aagtgatacc aggttttcgt
      541 gctcattctc atccggaatt aaagacgtat ctatacggat aacggtatta cctgcatcca
      601 ctaactgttt ctctaatgta gtcagctttg cagacgtttt cttccaaaaa catttggtat
      661 caaaacacaa atcgtcgctc tgcacgtcac caaacaatgt cctgttggct ccagaacgtt
      721 tcatgcagtt aatacaactg ccggcgcttt tattcaatct tttatcctca atgtcaaaag
      781 gtgcctttgt cagtaagcat acaaactcct tttcaatgat acgtcgcatt tctttcactg
      841 aacggtatgc aaaccggtgt tctccctcct tttcattgta tggtacattg tactttatac
      901 gttcgagaag ttccaactgt tttgcgtttt gcaattttgc aaactgtaaa caatggtgga
      961 gggtaatgtg gctgtcttta aataaacgct ttatctctgc attcaggctc tttaagtgta
     1021 ttcggtcata tacatactgc tccgatacac caatggttga tgcaatctgc tctacggagg
     1081 ttattttcgt caactgcaca aacgtttcac tctcgtcaat agggtgtggt tgctctctct
     1141 gcacgttttc tatcagctgt atctctacta acttatcagg ttttacattc tgaataattc
     1201 gggagtcaat tttttcccaa cccagggact tcgcagccaa caacctacgc tcaccaaata
     1261 ctaaatgata tttacctttg ccggtatctc ttaccataat cggatgcaga agttgcaccg
     1321 atttcattga ttctgctaat tcaatgattt tctcctcgtc aatatgtttt cgatagttcc
     1381 cattgtgtac tattttgtca atctcaatag attcgacgtt actgaatgat tgaatacttt
     1441 tttttagtgc cattactgtc gtattttata gtttgtaaaa tgtagaataa acgtttgatg
     1501 taggctaaat atgcatcttt gggtttcaga taacaaatat ggtaattatt agctaaagtt
     1561 gcatgattaa aataaaatac gttatactca atctgttgtg ccataatatc gtatttctta
     1621 attcttttca tgtaacgctc atccaaacca aaataatcga aaatacacga ctgtactcca
     1681 tcctcaatgt ccttgaataa tggtagtaat agctttatcg gtttaatcat gtcaggcagg
     1741 taagcctcac taccgtcatg tatcaatgca cataaacgtt cctccggctc tgcgtcataa
     1801 ggtagcaaac tctcaacgag tatgcaatgc tctgcaatgc taaagaactc cggtgtttgt
     1861 cctgaaaaat gtggcttatt ggctaatcct ttgcatatat ccaatacgtt caccatgtca
     1921 ggacgtggat tgataaaatc aaacctcaaa cctgaatagg tagatatgca gttgtcgtat
     1981 gcggttacat ctgttgtcat gcgaacggat ctacttcagc gttttctaaa tctctcaata
     2041 ttttttcttt ggcctcatcc actacctctg caggtttatt tgtcagctgc tcaacacgaa
     2101 atgctccggc caatattttt ttacagaagt cttttgtatc tgcagtaaaa aattcctgtt
     2161 ggttcatcca ttgcacgtat tggatatgtt tacttacttg attaccctga aacttaccaa
     2221 atccgaatac gatatttcct acctcatcca ataagaactt accggctata tccacctggt
     2281 ccttacttgt tgttaaaaaa ttttgtactt cggtattatt atatttaacc atggactgca
     2341 ggattacatc tactgcatat acatcattta aagcatcatg cgctccgtca atatctaaac
     2401 cggtatgttg cttgtaaacg ttattcaggg tacgacggta caatgcttta tccatttgga
     2461 acacgtcgat aatatctttt ggtacatccc aatcaatacc acatcttttg aactcctctt
     2521 tcagtaatgg aatgtcaaaa ttcttaatat tgaagcctat caacgtaaca tctaccatgt
     2581 actgaaagat gtcgtttgct atttcaggaa acgtcgatgc atctttcacc atttcatctg
     2641 ttatgccatg tacgtctgtt gcatctaccg gtataggtat tccgggatta atgaggatat
     2701 ttcttgtatc tctcattccg atactgtttg tgtaaaccaa tgcaatctgt acgattctat
     2761 cagttaatac gttcgttcct gttgtttcaa ggtcgaaatt tgtgtttggt gttggtactc
     2821 tgtaactcat ataaactttg ttttgttgct tagacaaaaa tacttaattt atacaattat
     2881 acaaactatc tgagaaatat tttaaacaaa aaaatgcacc tctttcgagg tgcataagaa
     2941 ctaaaaaaac ttaataaata accaaaaatt tataaatcca ttttactaat tctgccttta
     3001 taataactac caggattgaa tgattttgct ttatcgttta ctacaataag aaatacatta
     3061 tttacctcta cataatccaa attatcagta ttttttaaat gataatggat agtggaagca
     3121 ttaatctttt cattgcctgt acttgctctc aactgcttta caaattctgc aatcgttacg
     3181 caatctgcaa ctaatatctg atgctctgat aaatttaatt tcatttcttt gattttgctt
     3241 ttatgactgc atacgtcgtt acacctatcg ctactccggt tactattgta aagtttgaaa
     3301 aaaacaactt tcttcttctt ttcctgtttt cctttttgag tgctttattc tcgttcttta
     3361 aaagtacaat ttgtttatct ttttctgaag ctattaatga taaattatct atttgtgttg
     3421 cttgatctaa aataatactg tcattaatat tatttttttc tacaactaat ttttcataag
     3481 tcaatgaagt caaaagtaaa ctatcgtatg actttgcttt aatatcgctt tttataagtt
     3541 ccaacgtact ctgtttgtca aaacagaatt tatcattaat ttttaccgga ggatttattt
     3601 gagaaatacc taatgatgct ttcgctacca aaagtatcaa catcagcaat cttttggctt
     3661 gtagttttaa taattccatt ctgtttttgt tttatgtaaa caatttcttt tgtaataaca
     3721 tttttcctcc acgtcagcgt atctatctta ctttgcagga gtttgttctg ttttgtcagg
     3781 ctatcaatta ccatttgctt ttcagcatat atggccttaa taataccact taattctttc
     3841 gtgttatcag ctgaactctg aatgcgaaat acgatacctg taattatcaa tgttgctgcg
     3901 aatattgcaa tgtaaatcca atcacgcttt acaaatgcaa agatttcttt ttttatttct
     3961 gttttcattt tatacgtatt taataattaa aattaataca gttgcggtaa ttccgtacca
     4021 caaaaaatat ctcgacaatg tttccaactc aaccattaca gtatttataa tcaacagtga
     4081 ttccaaattg ttctctgctt cattcttttt ctgcaggttt gcataaacct ttgctaataa
     4141 gaaccggagt gaaataaaca cacttggaaa ccacgaaaaa attagtgcta ctataaacat
     4201 atactattat ttaaagatgt cctcgtctgt tacctgttct tcagtctttt cctctaccgg
     4261 agttgtcatg ttatcgaggt taagttgtaa ctgataatct tcagctttca aatcctcaac
     4321 gtgcaataca acgtgagtgc caggctccat gtaaattctc tgcttattag caaagtcaaa
     4381 tgctaaaatc gtatctacat tacgccactc atatccctca cgatacttag atgcaatgat
     4441 cgatatattc gtttcggtat ttttaacgat gtcagcaaga cgtttcgttt cagctttctt
     4501 catttcctct tgctcctctc tcattttttc gaactcacat aaccggtctg ctaattccaa
     4561 tttttcctcc ggtgtaaatg tgtatttgat ttctttcagt tggttagaat ggtattctgt
     4621 cacttcattg cctaccacct taaattctaa ggtaatgtcg ggataacaat actctctgat
     4681 ttggtcgaat gtaacatcag acggaatgat gtagtttgga aacggctctt ttgcaaagtc
     4741 gaatacgatt tcctttgagt aattctcttt ggcattttct accaactttg agattacctc
     4801 gtctaattcg gtacctgctg gtgccattaa gatttttcca tttgaaaata acttctgcac
     4861 aaaatgtttg tttattggtg attaaataat atcctctatt gttactaaaa ttttctgttg
     4921 tttcaacgtg tcaacctgtg tttgtttcgg tatgaactct accacgattg caggtttatc
     4981 atctacgata atcttgttgc catgcaatgc gtcgcctatg aatttgaatg cacctgcata
     5041 gttatcccaa tccattaatt tcactgcaaa cctgtggaat acgatacgca ccttacctgg
     5101 gtgccgggaa actgtctgtg agcgaatttt ccattgtatc tgttccaaca tctttgcacg
     5161 tttagtccaa tgcatacgga gcaggatatt catactcgga acatcactat tttttaaaat
     5221 cactgttgtt attgccattg ctcaaagtta attaagttta tatagctatc caaattatcg
     5281 ttctgttttt ttataacaaa gtataaccgt aatcctgtta tgtggtactt gattgctttc
     5341 cggattcatg gactacgttt atacgactgt tataagaaat gctacgatac atctccaaat
     5401 aaactttgtg gtggaaaaga attattaaaa attttccctc ctttttcttc ttcaaattta
     5461 tcagtttcgt taccccaaac atcccatcct tctgctcttg ttcgtgcaaa catttcaagt
     5521 tttggaacat cgccaaacat tcgttcaatc cgttttctaa attcgtttgg ctttttacta
     5581 tgttctgttt tattttcttc caagtatgat ttctccttaa aagatttcag taatttatgt
     5641 gctgttcctt ttgtagcaag taggcatatt tcacattgct ttacagtcca tttacccata
     5701 aaaccacgct ttttgttcca agtaaaagca acggttttgt atttgaaccc ccaattattt
     5761 attgccttta ttgctacttc taaatgagca tcagtagtcc atacaaacaa aacacaatta
     5821 tcatcagcat aatcggctac attcaattca attatttctt tatcggacat agtaggatat
     5881 tgtattgata attcctcatt cgttccaaac ttttctctta tgtcttttcg cctatttatt
     5941 ccttgcgaaa atctccaagc agggtcagca taaataacat tatatttttt gccatcgcac
     6001 atttttaata attcttttct ttccgtttcc atattaaatt tatcgtttaa ttaaccgcac
     6061 ttcttataac agcagttagg tgcaatgcta aacgacaccg tcacgataaa atttcttatt
     6121 caaattatga caagcaatca tcacatcttc ataagcacct ttatatcttg tcttattttc
     6181 attatgtgtg tctattactt cccatttgtt atttatgcaa atcttttgaa accttctttt
     6241 ggtgcttaga cttggcattt ctactttttc tgttattatt tcaatcatat ttttgaattt
     6301 ctgagaagca ctgcacctaa caaggtattg ccaaaagcag ggcattctcg gttaattaat
     6361 cgtttgtact tctattgggt atttgtgcaa ggttgaaact tttgtctttc aaatccctgc
     6421 cttcggcaat acccgaaccg ttatgtgcaa ggctacgaaa ctgcatctaa acgagcattt
     6481 gtgatttcaa tagcctttgg attaatgtcg caacctataa aatttcggtt aagttcttta
     6541 caaactactg cggttgtgcc actacctaaa taataatcag caactgtatc accttcgttt
     6601 gaacttgctt ttataattcg ttcaactaat gcctttggtt tttgtgttga ataaccaatc
     6661 ctttcgttac tcatcggatt aataaaagga atacaccacc aatcttcagg gattttacct
     6721 tcttcgtttg gtttccaatt gtgtacttta ccattgatat ttttatccat tattttaccg
     6781 cttttcaatg ctgtatcagt tccaccacct attgtgtatg gtattctaac ctcattaaaa
     6841 gtgtaatttt tagtttttga atatctataa attacatcgt gtttttctgc aaaatctttt
     6901 ttctttcttc caccacccga atagcaccaa attatttcat ttctaaaatt accatattca
     6961 aaaacttcat ccattaagca tctaatccaa tggtttattt tagtatccat ttgtaaataa
     7021 atacttcctg tacttttcaa tactcggtgc atttctttta atcgtggcaa atagtggctt
     7081 tcaatttcac ttctaatcgg ttttaagtct tggtaatctc cgaaatttct accagttcca
     7141 taaagaatat cgcaataaat caaatctaca ctttcattag gttgcgacaa aaggagttca
     7201 aggttatcaa ccgccctgca cataacacgg gtttggcaaa atggctgttc agtaattcta
     7261 tcaatcattc gtttttaatt attaagtttt gtaattctat ttaagttcgg gttcagccac
     7321 ttcgccaagc ccgaaaacgc tatacgcaat ttccccaccg cacaaagccg acacacaact
     7381 gcgtatagct gcgaaccgtt agcaaacagc ttaataccgc ttaccatgtt tgaaagtgcg
     7441 agttgaatta tatcgaagtt ttaaatcaat atgtttttca atatcaattc ctaaaccacc
     7501 acataggtcg aaaaggcgaa taaatgaatc tgcaatttca tcttcaaaag tatcttttac
     7561 agttgtttca aatctgtttt ttaaccaatc gcaattttcg ttttcagttt caaaatctga
     7621 aattgaaagt tttgcatatc ctaaatcttg ttcaaaggca tttaacattt tccttttgtc
     7681 ttttcggtca gcctctaaag cctcagcaag ttctgaaact acaagcatta aagtttgtcc
     7741 gatattttct tttgaaacat cgaagccacg aagtttattt ccttcgtaaa tttgttttga
     7801 taaatcattt aagttgttca tataaaattg attttaaatt aattactaaa aaaaagccga
     7861 tttgctaaca caagctataa ccaataaagt ttttagtggt taattcagca ttgtagctcg
     7921 catcagcatc ggtgtatttt gacaggtacg aagcccgtaa tactttactg gtcatagctt
     7981 caacgttagc accaatacta cgaatgtgct tcgaaaactt gattatccct taatcgtatt
     8041 tgctctctta tcttttcggc taattcttct actttatcag aataagaaca tataactgtt
     8101 ccgtcatctt tttttaaagc tatcggcatt gcatttgcgt atagttcaaa tagtgcctta
     8161 aatagttctt ttctttggct atcattcaaa atagcctctc tttgtttttg gtaatacttt
     8221 tggtcttcca ttctatttaa attttgttgt taaaattcgc actgcgccaa tcgctttgtt
     8281 aggcgtaatg ctaaaacagc gttccattca aagtttcgtt agctaaccga caatagtttg
     8341 aatttatttc gctaccaaag tatttaagtc caaatgacct cgctaccttt aaggttgttc
     8401 ctgttccagc ataagggtca tacactacaa aatcttcggt tttatcctta atacacatca
     8461 taatattagt tggcaattct tctggaaatg gcgcaggatg aagcggatta ggtttagccg
     8521 aaaactgcca tacttcacca acaaataaag gtgcttttgc tctttcaaag ttaggttggc
     8581 aaggtgtttt agttagccaa aaaatcaact ctgtattagg caaatatcta ataggtgcaa
     8641 ccgcagggct attttttcta tcccaaatta tttgctgtct aaaagtaaag ttgcttttta
     8701 atatccattc aatcggatgt gatgctttgt gttgtgccac cctaactttg tggttgtaaa
     8761 atacacttcc gtttggtttc aatattctgt gcatttcatt aagcaccttt atttgttgct
     8821 cttgatattc ggcttcaatc ataaagtcgt tatccgcttc accgccatac tcaatatttc
     8881 tgcctttgct ccaactatct ttttcgtgtc ttttacggat aaaaccttcg taaccagctt
     8941 tgttataagg tggagaagta attatcaaat caacggaatt gtcggttagc tttcccatcg
     9001 ttgtaaggca atcttccaaa agcactacgc ctaacatcgg ttttgtgcaa gtggggcttt
     9061 ctactgtatt catcatttgt ttttcaattt aagtttagtg gtgggctgaa catttcggct
     9121 tcgatttccc cacctgcaca aagcccgaaa acggcgttat aaaattatca tactatcatg
     9181 tttattcaac ttttcctgtt cttccaatgc tttcactatc atcttgtatt ctctgtcata
     9241 atgccattga ttttcaggat tatttttgta atcgtcaaat tcttctatta taatttttac
     9301 tgcaattttg tttgtgtgtt ccatactttt taatttatac gtctatcaat actgtcagat
     9361 ttattgccta agtagataac attgcacatt ccgcataacc tcgataatgt acggttgtcg
     9421 taatagcttt caatctcact caacattaga tttgtcgtaa ataatgaacg tgtaccatag
     9481 tcattgtata atcgttcacg ctttataatt atatccatga ggcagtttac ctcggtgccg
     9541 taatccttga acttcttttc aaatcccaaa tcgtcaaaca ggaatatgct ctctgaagta
     9601 tcatgctgaa tacgctccaa atttgaattg taatatttaa acaatgattg catacccaac
     9661 cgtcggtaat cgtccgttat cacgtcgcaa tacgctatac ggaagtaatt ggacggtatt
     9721 ctcatttctt gaaatatccg gagtagattg gttttaccac taccgacgtt accggccaaa
     9781 atcaaacctt tctctaaatc aggcttatac ttcatcacat tgtttggcgt gatctcattg
     9841 aatgctctgt tgttgctgaa atacagtaac aggtatttta tctgttctct gttctcatca
     9901 tctatactga atactccttt ccggccatag tgcccggcca ccgatttgaa tacttcgagg
     9961 aatgcatcaa actgcatttg ttccctgtta gttaaattct tttctttggg agttgtcctt
    10021 atttccgtca gcaggtgttt taagttttgc attttttaaa tcggttaata ttttcattaa
    10081 atcgttatct atctctgtca acttcacacg tttctgcatg aaattatcta acttattcca
    10141 attcgtcagt atatacctcc acgatatgac ggctccgtcc ttatcggtaa attctgtaat
    10201 ggaggtatct ctattgtcgt ttcttttttt gttggcattc acattgtttt cgagcaaata
    10261 accaatgatt gtacgcatag aacgtctatc aaagtcggtt aactttactg catgaatatc
    10321 cggttcctgt ttcacaatga aatcacgata catcttctcg aatatcggtt ccaactcttt
    10381 cacttcaccc tggctaaaat tctttttcag gtacgtcaat aatgtcaggt aaatcgtttg
    10441 cgatttgttt gttgctttgt gatttgccat tgctatcagc atttcctcta tctgttcaaa
    10501 ggtaaatttt tctctcaatt tttcatactc atctaccgtc agcgtttttg gtatcttctg
    10561 cagtattggt tgttcgaata tgaatttcat cacttttaaa aattcttttc gatattcttc
    10621 tttcaaaatt ttttctgcag ggggattaaa tccgtcgaca gacggaatgt ttcccttttc
    10681 atcattctta actgtattat ctttcttaga ctgtattaca gtttcgttct gtttctgttc
    10741 ctctactgtt ttatctccgt tctgttggtg ttccactact gttctaacta ctgttccaac
    10801 gtcgttccaa tcagtattat aactatcgta attacaaaga cttataactg tttcaccctg
    10861 ttccgttctt gttgcaatca tgtcctcatt cttcagtaaa tctaagaaac gtttaacctg
    10921 tgacggtcca ctccaattcc accgctcatt taggtatctc gacgatgcag gaaactcacc
    10981 tcttttccat tcaatcagct ttccatttac tattttttta ccattgcttg ttgcgtcata
    11041 tctcactaat tgtatcaaat ccaaccatgc ctcggccttt gagaatttac gtttctcatt
    11101 ccataggaaa ttattaaaat actttcgtga caattttata taaccgctca aaacttagat
    11161 aattttaatg atataatctt cggtaggtat ttcattaggt tcaggcagat agaactcaaa
    11221 tccggccata aaacaacgga tttgctctaa atattcattg tgctcaactg tcgttaatga
    11281 tgttggccga atgatgccta aattaccctc ctcatctact gtgaggagga ataatttagt
    11341 cattatctca tagacttcat aggcagtatg tcctgtccat gaggctatca acctgtaata
    11401 aaaccactcg ctacgatgct gtcgaacact tctcgacatt cgctttcggt tttactgtaa
    11461 cgtaatctaa caaatcatgt cgtgtgcaaa aatcaatgta ttccctgcaa agtgaataca
    11521 cgtcattctt caggttctca tacggaaaga atgtaaaatc taccggctca tccacctcat
    11581 accctttttt ctcatttcct ttgtacttga atacattgta aaccactttc tgcacccctg
    11641 ttgctaagat gtagcatttc cattggattg acttatagta tttctcgaaa ctaacggcct
    11701 tatcggtcgt cttattctcg ttcactatgt caccctccaa actatctact ttcagcgaga
    11761 atacggcaat aaatgcaccc atatccaatt caaaggaatg cttacattca gataccatac
    11821 gtgaatgatt gttcctgaat ctgactgcaa catcaatctg ttcctgttcc atgactacgg
    11881 cctccggaaa gtcactttcc ttgacaatgt atttaccact atcaaagtca taatactttt
    11941 cagggccgaa ctcaattact gcatgaaagg cgctaccaaa attcatcttt cgactccact
    12001 ctttagtacc ttttatttca gcaatgaaat cctccgtcgt gccgtaatgc tcctcgatga
    12061 gataccggaa actttccaac tttgacggag taattttata ctttggcttc agcgttttca
    12121 tttttgaata gttcagttgt tggttcttca gctttctttt ttgaggcact tttcttcact
    12181 tcctctttca cttcctctgc aggagcactc gtctgttcgg caggcacttc ggtaactgca
    12241 ttaagcaccg gctgcttatc ttcctctttc acttcctctg caggagcatt cgtctgttcg
    12301 gcaggtactt cggtaactgc attaagcacc ggctgcttat cttcctcttt cacttctgca
    12361 cctggttcgt ctttaacttc aggttttaca aacacaccat tatctttgtc aaactcaaaa
    12421 cccaaccctg ttttacggtc attaagcagt cttttgtaga agttactatc accatatttt
    12481 gtcttaatct cattcagcat cacatctaac tgcaacggtt ctgttaaccc tgaaatcatt
    12541 acttttataa actcagtctt tttatcctca acaagtttag ataactgaat ttggaatgca
    12601 ggtttcaaat ctgcaaatgt ttctgttaac gctaccaact tttcgaatgt atcggcttca
    12661 tcaacttctt tacgtttctc atccaaaagt aataacgctt tttgctgctc ctcatccaat
    12721 ttggacattt tttctttggc atcagcaatg atacggccta aaaaattgtc aaactcaata
    12781 gtttctgcat gcggaatgat gaaattagca aagtttggtg catttttacc gatgtgacgg
    12841 tcagtaggat tgaaattcag tgtacgtttc tcattcttac tttccatgta accaaccaaa
    12901 tcagctacct gcataagaat atcgtaacta ccacctgtca ttttcggacg gaaacgcaga
    12961 ttttcaccgt ctttgtactg ttccgtatgg catacgataa caatgtcaat ctgcattcgt
    13021 ttcaattcat taataaactg actgaatacc tctttcaatg caccataacc ctgaagtgat
    13081 aagcctccac ctttcttttt gttggcagtt tcattttcga ttacatagtg tgcaatgtag
    13141 ttgtccaaca acgtaccacc tgtatcgaga attacggtac gtggtttcaa ttccttaaat
    13201 ttctccgaac ggataaaatc caatgctcct gcaaaagtac gcatttgcac tgcacgttta
    13261 cgttttgttg aacgctttaa tccgtcgtca aagtccaata aaattgattc ttcactctgc
    13321 attgaaagtg atgtctttcc catgccggga tctccgaaga ttactgttaa aatagatgct
    13381 atcttcaacg catctgtttc ctcaacaatg ttccattgtt gttcttgaac gatctgttcc
    13441 ataaataaaa aagtttattg gtgaaaaaat aaaaagtttc ataagaagtt gttgctaatg
    13501 caaatctaat taatttatac aactatacaa atctatttcg atattttttt cagaatttta
    13561 tctttcatag ctgaaattac agattcttca ctgttctcaa tgtgctctga accaccgacg
    13621 gcctctttct caatcacacg tcgtttatca attatctcat acgtgtcctc atccaatgta
    13681 tctttaccga gaaatacata cacgttaaaa tcttcagttt tattaacggc aatgcctctg
    13741 tcctccattt gtgcctggtc tttgtatgtc caacctaatt caaccataac caaacttgat
    13801 gccttgctca atgtatgtcc ctcaccaccc tttccataag taatgataat aactttcgta
    13861 ttatcgtctg tctgaaactt tttcttgttc tcatggattt cttcaggttt catatcatgg
    13921 ccgtcgattc tgccgcagat agtaacggct ccatactccc ggaaagcatc tttgaaatac
    13981 tgaacggtat cattgtacca acaaccgata atcactttct ctccgttatc tatcaattca
    14041 gatacaaact ccttgacgga tgctgattta cctctcgcag aaatttgctt cagcaatccc
    14101 attttaacca acatttcagc ctgtaatgct ttcgatatct gtgaagtggt gaatgctctc
    14161 tctgccatgt atgactgaag tgataaccaa gcatggtcgt actcgctctg attatccaaa
    14221 tccactcgta tcacctttct gaacttctcc gatatctgct cattaacctg gtgccgttcc
    14281 cgacggatga aacacaatga acgcattttt gtattcaact cctgaagttc ctgcagttgc
    14341 ttacctttgt cgtctaatgt ttttgcatct atttttttgt acttcttaat gaatttgtca
    14401 tatcctccga actcctcaat acggcctatc agttcaaaga gtgcggccaa atctttcggg
    14461 cctttcacta tcggagaacc ggttaagcat atacgaacct ctttaccttt gaatacttca
    14521 cgcacacact tgtaccgtat cgtctgcttg ttcctgcaat gatgtgcctc gtcaatgatt
    14581 gctgaattaa aaagattgca taaatcattc ccttttgcat tgtgatattt cgttcctgca
    14641 ttcgggccgg tagttatgtt gacttctttc aaatcatcta tcaggaatga ctgtacgccg
    14701 tcgtaattcg taataacgac atctgccata ccctgacgta tcagttccgg caatttcttt
    14761 acgtttttat ggtttaatac aatggctttt ctatctgtcc aatcatgcca ctcatcctgc
    14821 caattcagcc tcaaagattt cggaacgata actatgcagg gaaatgagtt tgtaaccaaa
    14881 ataccggcta ttgattgcat ggttttacct agtcgcattt ggtcggcaat aaccacacgc
    14941 ttattccgtc gcacatagtc accaccaacg gtttgatacg gatatagttt tttctttagt
    15001 ggaatattca actctatgta ctccgtcatg tatgacaact ccatattctc accgctctcg
    15061 tactgcagat tgattaagtt catggctctt tcacctatct gaaactcata tccattggca
    15121 aaatgtgtta cctctttcgc aaattccaac ggaactatcc accgctttgt atcgtacatg
    15181 aacctggctc tcggtatatt tttgactgca tcgtacaatt ctttacggta cggaaacgta
    15241 atgataaaga aattaccgtc ggtaccgata agcctcctct gctccggctg aaccacgtta
    15301 tccctgttca ctactgataa ctgtatctgt attacaggaa ttttgctaat atcaaaccct
    15361 acacgtttca acttcaggcc gtatggttta atgatattgt gaagccgtat cgcctcgtca
    15421 atagtccatg ttttataatc tttttgcgct ataccacgca gaaattttgc actgatacta
    15481 acttcagttt ccggtaaaat tctttgcttg aataactccc taaacgcatt caggccgtta
    15541 tataattcgg tgaaatcttg catatacttt attgttgcta cacaaaaata gtaatttata
    15601 cggttataca aaataaaaat gcatagaatt aatctatgca ttttcaccgg tgccgggggg
    15661 gaatcatacg aggtaattta gcaacaacaa agtttgaaac caatagcgac cggctgactg
    15721 taaaagtaac gctttttttt caatcgtgca actttacagt atttctacta ttaaatattc
    15781 gttagtatct tcgtgataaa tgatataatt tatcggaatt acgttttcta aaacttccaa
    15841 ctgcaggttt gctctgtatt cctcaaagta cggaaacaac tcgtgaccgc ttgttaatct
    15901 tgttattcct gtgtgattgt tcagaaaatc tactaccaac acattgaaat tataagtctg
    15961 tggaggatcg acgccattat cgtaaatctg tggacttagg tagaatatcc ctgcatctgc
    16021 aactgccgga ttggtattgc tattgcaacc ttttgcatct atgtaaacag aagttggcaa
    16081 tgttgcagtt aaagtagcaa actttgcact cattttcgac aataaatttg tatctgcatc
    16141 attaataccg gctgccaccg caacaacaat taaaaataac tgaacgtcgt cgtctattac
    16201 aattccctct gaagtcaata ccgggcctgt tgtcacgtcg atattaaaga tacctccatt
    16261 cgcatagatt ctttcaatta ccgtatatct ttctgaattt gtcatattat ggaattaaaa
    16321 gtattggttg ataaattccg tatgtattcg ggattgccgt aagtgcaatt atctgcatat
    16381 cctgaaatgt tggtcctatt gtaaaagata aatgacctat tgaaaatcct actccatttt
    16441 gtttataagt aaccggtact gccttactcc aaacataacc atttaataca ccaacaaaac
    16501 gtccaataat tgtacctgct gcgatatctg ctgaaactcc aaatgaaaac tcaacaatca
    16561 tcattcctaa atcgttttta cataatcgca tagccggacg tgaagcctct ctcggactta
    16621 tagatgaatg cgtgaagtct gcagttagcc acgttactcc tttgtcaatt ttcttatcgt
    16681 tcaacttgtg agcatagtac gcatcacgat ttgcagatac tatttcccaa acggtatctg
    16741 tctttctgaa cgtaatcaca tcaccgcttt ctccgtaaat ttggtcgtct ataattgaca
    16801 acgatttacc caaactacta atataaccaa agtcgctcac attggaacgg atatttgatg
    16861 tgttttcgag tgaaataagg aacggaactg cagtcaatac gcctccacta cttgcaatag
    16921 aacctcctat atgcttgaaa cgtatattga taattgtacc tgggcgaaca tctaccgtcg
    16981 caccataatc atctaccacg ttaatattct ccaaactacc tgcagttggt actaaaatac
    17041 taaaacagtt atttctatta tttacagtaa ttgttttcga taatgcattg aatgcagagt
    17101 tatctaatac aaaataaccg tcgtaatgtt tcttctctcc ctgaacggcc accacacgat
    17161 atgctgaagc ctccgctttc aaccaaacac aaccaccttt gtttactacc aaatcacctg
    17221 aatttgttgt gtaaattggc agattgcctc cacttgctac aaagtcatta tttttgaaaa
    17281 ttacatggtc caacgtaata cctgcatccg acaaatcaat tttgataatc gtatctaccg
    17341 gcaatgcctt aaaaccggtt acagatgtgg ctcctactgt gctcggagaa cccatagatg
    17401 taatcttcag gatatttccg tcagttgcag taaatgaact gcttttaccg tcgtcaccaa
    17461 tcgtgatctg ttttgtattt tctgtaacgg tacctgaaaa cttattcgat gcagtaaaat
    17521 tgttgctcct gttttcagat gctctttgtg cattcaccaa tacccactta ttgttgtctg
    17581 taactaccgg agaggtattc gatacgcttt taagtacaaa aacgctattt gcaggaatgt
    17641 agcaatacgt cgcatcaccg tcgctcggca atatgtcgta accaaaatca atacgcaact
    17701 cctgttttgt ataaatcgtc acttccttac cacctaaatt ctcatctggg ccaacgtaac
    17761 gcaaccttac attctcattc tgtattccac ctgtgtaacc ataatcgaga atgaaattat
    17821 ttgcatcctt gtttacggca ccggccggcg cactgtttaa gttcaaactg aaataatata
    17881 caggatcgtc gttaccaaca ttatccaata cgcaattacg aaccattccg ataataccta
    17941 aaaagatatt cagcaaatat tgatgtgcta tactgccgtc acctgcaaag ttcggagtta
    18001 ctgctctcgt atatactact cccggagcat ccatgtattc tgcaccgttt ggaacgtaca
    18061 gataccctaa aattgtctgt gttttgggac ttgtcaacgt cggagcaacc ggagttcctg
    18121 caggagtacc caacaatact gaatatacca cgtttgaacc gtccataacg tccacgtatt
    18181 catgctccat tatcacaaca tctattcgag gatttccgct cgtatttgga gtgaatggca
    18241 acacaacaac atcgtcctca tgtatcacta ctccctgctt tgtaaccact actcctctct
    18301 gctctgtggt gaataccgga gaatctgtaa gcacaatagt tgcacctgtt acagtctgaa
    18361 ccaattttat atttgaaaca tctgaaaggt ctatatcaaa accacgatac aaccctggct
    18421 ctactatacc taatgtacgc tgattaagat gtttggttga atcatcgtct tgccaattcc
    18481 ataaaatctt ttgtgccatt ttattttaat ttatcttcaa ttaaaaactt aaatactttc
    18541 ggtattagat tagccaaccg gtagttgcag aaacctaata cgaaacctaa acccattgtg
    18601 gcctgtgctc caatccaatc attgaataac ctcatacaga tacatgctaa tacaaatcct
    18661 aataataatt ctgcaatttt atctttaaac atatacctgc tgctgaattt attcggagta
    18721 cctggcatct taacacctac tctcaatgct ctaattgtta ctgaaagaat acaacctata
    18781 acaacaaaaa atacacctgc tattgcaaat gcaatatcgt ctgtacctac tatgtattca
    18841 aacgtttttt gtaaaatttc attattattt gctgtttcca taacttagga cttttctttt
    18901 aatgtttcaa tttcttttct tgcaaactca tgctctttca tacatttttg gtagttttct
    18961 ttaataacta ccaactcctt ttgcgtattc gcaagtaatg aaagtgcatc agtagagttc
    19021 ttcaggtaca tttcctgtcg ttctctaatc gttcttagat tgattttatg ttctgcaatc
    19081 tcggtacgca attcggactt tatttgctca tcgagcaatt tattatcctt gtaagagtta
    19141 accaatattt ccatgttctg catgataata tggtcaacgt cagctgtgtt cttccgtcgg
    19201 tacataaaga aatttacgac ggtaactaaa ataggactac taaaaaaaat agtaatcaat
    19261 gcaatgatta aagggctcat gtttttaata tctattacca ttaattaata agattttcgt
    19321 acttaataat tatttcagca atcttgtcag caagtgtaaa cttgttgtcc tgatacttct
    19381 tcaaatcgga ggcattggtg ataaaacata tttctaataa tgcaacaata cctctctctc
    19441 tcatcaatcc taaacgacct ctgtgtgatt cgctttcaga aatacaacca cgatttttaa
    19501 tacctaagat ttgtgcagtc gcctccacaa gttcgttgcc aaaatgctta tcatttttat
    19561 ctgcatcact tccaatcaat gacgtgcaac ctgtggctgt cggatttatt gcagcgtcga
    19621 aatgaaactc taaaacgacc gaaccgttac cggtaataat acgcttcaga tattgtccta
    19681 acgtttcact atcatcgtct gcaatacctt taaaacctct ctttaaaagg ctccctaaaa
    19741 caagattacg gaactcaacg gttaagtcag cttcacgaaa accattgcca acggctcccg
    19801 gatctttctt tattccgttc ggattatgtc ctgctgaaac aaatatcgcc atattccatt
    19861 ttttataagt ggagaataaa ttatcaattc aatataaata aaaagtgcca ctttcaaaag
    19921 tggcaactaa ataaataata agattgaggt ttattattat gcttctaaag gactatatag
    19981 tatttgtaaa aaatagtatt gctaatacta ttcctgttaa tccgaataaa atcataaaca
    20041 cctgtaaacc tgttttgatt ttaagtttct ttgcaaagaa agaaacaatt actaacacta
    20101 atgagattag taaaaagaca attcctccaa tcataatttt ctgtattttt tagatgtgta
    20161 taatagatga aatgttgccg ttcttacaaa ataaacagac agcaacatga atgaatagta
    20221 aggtgtaatt ttacctgaaa taaatccaac gaagaaacct gaaacgagca aacaaatagt
    20281 aagaacgaat tggtatttat gccaaccatc tgtaaacatt accagcaaat ttttcattaa
    20341 ccataccttt actttgcctg tgtattcgta cttattttta ctgcttaaat gtttgtgcca
    20401 atattcgtat g
//
LOCUS       DAOOOL010000035        20000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_203_length_20000_cov_105.727350, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000035 DAOOOL010000000
VERSION     DAOOOL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_20000_cov_105.727350"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKU82_04620"
     CDS             <1..663
                     /locus_tag="PKU82_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOZ82192.1"
                     /translation="YAKMAKAFTRIGKEIAIAVRQGGPDPHYNPKLRQVIQNAKGLNM
                     PKDRVDAAIKRATSKDMKDYEEITYEGYAPFGVAIMVECATDNSTRTVANIRMHFNRE
                     EGSLGTSGSVGFMFERRGLFKFSAEGKNIEDLELELIDFGLIEIWEEDGFIYLYCNFE
                     DFGAMTKVLEDRSIEIINSELTRVPLTLTEVTEEQEHKIQVLIDRLEDDEDVQSVFHN
                     MK"
     gene            687..1817
                     /locus_tag="PKU82_04625"
     CDS             687..1817
                     /locus_tag="PKU82_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOZ82193.1"
                     /translation="MIIGAGPAGSACVMALKDAGLNIVWIDKATFPRDKVCGDAIPSN
                     VQRVLKTIDVSLNEKFLQQFIEKVQVEGCRFVAPDKSSFDLTFVTKGHAAKRLHFDHF
                     LFDMANECNSHVTFIGNASVELIVNSATAVTVKLTDGRTIEGQIIIGCDGANSIVRKN
                     LAQTFIRKKENIAAVRAYYNNVADLEHNMLEIHIVKGYMPGYFWVFPLPENQSNVGFG
                     MVNRFITNRKIDLKEALKEIIQNDKAISPRFSQATTDGVISGFGLPCGGRKNPISGQR
                     FLLCGDAASLINPATGEGIGNAMISGRIAALHIINCFQANNFTQSFNAQYDVLVYDKL
                     LGDLRIQHWLQRITADREWLINFALKHVSNNNWIRQKVRKFF"
     gene            complement(1821..3053)
                     /locus_tag="PKU82_04630"
     CDS             complement(1821..3053)
                     /locus_tag="PKU82_04630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ82194.1"
                     /translation="MENVDVLIIGAGPSGAVASAYLNKQNIKVLVLEKQKFPRFVIGE
                     SLLPHCMDHLSEVGFIDNIKKHGFQEKTGAAFYKGKHKTEFFFNDQFSDGWSWTWQVK
                     RADFDNILISTAIEQGVEVKFECEVNSVAASASEQTTTYTDKSGKTHTVKSKYIIDAS
                     GYGRVLPRLFKLEEPVSTPPRGAVFCHIHDTKRTKKAGANIFIHSFNENSAWIWAIPF
                     SDKTTSVGVVSNVELVDEFAANNGEKFLNHIRTFGDLAGRFENEQLVFEPRTIHNYAV
                     RSKQLFGPGYVLCGNSTEFLDPVFSSGVTLATGSGLLAAKLTEKVLNGEQVNWSEVYE
                     EKLRRGIDVFRTFVLGWYNGDLQTIIYSNDIDPNIKKQICSVLAGYVWDTENPVVKKH
                     KTLIPTLAEVIRIKEKEA"
     gene            complement(3113..4660)
                     /locus_tag="PKU82_04635"
     CDS             complement(3113..4660)
                     /locus_tag="PKU82_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ82195.1"
                     /translation="MKINDKIVYDIAIIGGGFSGMCCAYILAKEGYKVALVEKNIQLG
                     GSLQIFSRDKKILDTGLHYIGGYDEGENLYQLFSYFGLKDTLQTKRMDDNGYDYVSFS
                     DGRKYAHAMGYDNFINSLCKDFPDEEKAIHRFCDKLKEYCQKFPLYDLSNKITDDYFN
                     EELRNENAWDYIASITENKTLRNVLAGNNMLYAGIKEKTPMFVHALIFNSYICGSYKI
                     VTGGAQLIKQFASNIRKAGADIYTHKQITGATYKENREISQLLLENGECITALKYISA
                     IHPNETISLLGNDRFSHAWVNRVKQMDQTVSAFLVQITFKPKSFPYMNYNMYHFFNDD
                     VWDTINYTEDNWPLSFFVFTPVPLKDPQYAESISVMCYMKSDDVAQWKDSFNTTTHPS
                     NRSESYQEFKRRKEEKVLEKLETLFPEIRKAILNVYSSTPLTFKDYTASTDGSIYGVL
                     KDSNAPLKSMLQPRTKIPNLWLTGQNIVFHGILGTAISALLTCFEFTDKKELLKKINP
                     EKNHIQT"
     gene            complement(4657..8472)
                     /locus_tag="PKU82_04640"
     CDS             complement(4657..8472)
                     /locus_tag="PKU82_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOZ82196.1"
                     /translation="MGSIFIGLYRLFVRKPILFAGFVLLVAAVSIAGIKRLNITENIF
                     AILPKGQEFQKFNSLLESKNISDQVVFSLSLTKNSNPDEYADVAEHVKSTIDSASANL
                     LSDVVAVRPDVQQEVYDYYFSHFPLLIDSNYYSLIERKIQDDSIQFSIKNNYERLTNP
                     SGLFIKQFILNDPLSITAEYFRQLGITGNSSNYVVEDGIIFTPGKKSILITGRLNYDS
                     RDAEKNIQLSDQLAAIEKKWNAENSEYKLSHFGTFEIAAQNAVQIKKDTNLTMILSLS
                     LIMLILILYYRKLSIPILFILPAVFGGMLALGLMGFLRPEISAISLATGAIVIGIILD
                     YSFHFFTHLQHVKSIEETLKEISVPLLTGSVTTVLALLALNYTNSVVLGDFGLFAAMA
                     LAGSAFFTLAGLPVIITLTRFDYTQHKTIKWFKLPVINPSIVKYAIIAILLITVYFYF
                     EASNIRFNSDVETLSFHPTELKNKEAELTGINPSIEKKIYLIASAKNYNSAVDANYKA
                     GTLIKNLITHGIIKNTLSTADYFIPDSLCKQRQLRWNYFWQQNKTRVTNQIKLSADEA
                     GFSNNAFAPFQEMIDDSIYQPLSKDLLKTMGLDLMFDSSAKESVFISSFTVATSQKDS
                     VKALFKDIPHLTVFDRSEMASLLLESVRSDFNYLLYLTAGMVFFTLLLIYGRVELALL
                     AFLPMVVSWIWILGMSSTFNIEFNFVNIVITTFIFGLGDDFSIFVTDGLLNRYKFRKD
                     NLKSYQAAIILSALTVIVGTGVLYFAKHPAIHSIAALSVIGITCILLISFLVQPFIFK
                     LFVERRIKNGHNPISFLQLFMSVFCFTYFIVGCLTFYPVLLLISLFPAPVKIKRKAVN
                     FIVSKFSASVLYSGIHVKKKFYNLDQLDFKKPSIIIANHSSFLDIMIMLMLNRKVIIM
                     AKDWVYRSPLFGLVVRYVGYIFSEDGTEDNITNIKAKMDEGYSIMIFPEGSRSSDGNL
                     NRFHKGAFYLAEKLNADIQPVMIHGAGDVSPKNEMMVKDGTINVKALPRIFANDLSWG
                     NDYRSRQKSISQYFKEQYRLFKDEREDADYLWQKVYYNYIFKGPVLEWYGRIKWNLEK
                     KNYDFYNNLIGQRILITDLGCGYGFMDLFLHYKNRERKISAFDYDDDKIAIAANVWNK
                     NANLQFFAADITKTEVPSSEVIFLNDVLHYLNIDSQEKVLQKCADALMPGGIMFIRDG
                     VADDIKHRNTKFTELLSTKFFSFNKKMNDLNFFKTEYIYQFAKRNNLKIEEHKHSNNT
                     SNRLFVLSK"
     gene            8607..9701
                     /gene="hppD"
                     /locus_tag="PKU82_04645"
     CDS             8607..9701
                     /gene="hppD"
                     /locus_tag="PKU82_04645"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOZ82197.1"
                     /translation="MAEDFLPLNGTDHVELYVGNAKQSAYYYQMAWGYELIAYAGPET
                     GIRDRASYVLQQGKIRLVLTSAMHPDSEITRHVAQHGDGVKFLAIWVDDAEKSFYETM
                     SRGAKAHTQPTVLSDKFGEVKISAIHTYGETVHKFVERKNYNGAFLPGYSPAKSSVKT
                     TPVGLKHVDHCVGNVELGRMNEWVKFYEDVMGFKLLITFDDSDISTEYSALMSKVVSN
                     GNGYIKFPINEPADGKRKSQIEEYIEFYHGAGVQHVAIATDDIISTVTELRNRGVEFL
                     HVPEVYYEDVWDRVGKINEDVATIKKLNILVDRDDEGYLLQLFTKPVQDRPTVFFEII
                     QRCGAKSFGKGNFKALFEAIEREQALRGNL"
     gene            complement(9725..10345)
                     /gene="recR"
                     /locus_tag="PKU82_04650"
     CDS             complement(9725..10345)
                     /gene="recR"
                     /locus_tag="PKU82_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOZ82198.1"
                     /translation="MEQYPSRLLEEAVNELSRLPGIGRKGALRLALHLLKQNPADVER
                     LGNAVIQLRSSVVYCHQCHNISENTLCAICANPRRDGSVICVVQDIRDVMAIENTAQF
                     NGMYHVLGGIISPMDGIGPSQLNIDSLVEKTANGNINEIIMALSATMEGDTTVFYISK
                     RLKDIDVKISILARGVAIGGELEFADEVTLGRSLINRVAYENTLVK"
     gene            10457..11920
                     /locus_tag="PKU82_04655"
     CDS             10457..11920
                     /locus_tag="PKU82_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOZ82199.1"
                     /translation="MSPVLILSVVLVYTILLFAITFYTSRNADNQSYFVGNRSSRWYV
                     VAYGMIGASLSGVTFMSVPGDVGTGNFSYFQMVLGYLLGYAVIAFVLLPVYYRMNLTS
                     IYTYLKSRLGEKTYKTGAFFFILSRVVGASFRMYIVVYVLQLFIFQAWGVPFWVTVFV
                     FVLLILSYTFKGGVKTIVWTDSLQTTFMLLSLFISVYLIAKELNFSFADVVSSIKSSP
                     YSDIFVWDWRSKVFFPKHFFGGMFIAIAMTGLDQEMMQKNISCRNVRESQKNMMTFSI
                     ILVFVNFIFLCLGALLYIFIQHQGIVIPERTTDDLFPTIAMHYLGTFSAICFIIGLIS
                     AAYPSADGALTALTASVCIDFLGFEEGKYTEAQKTKIRKSVHVIFGFVLLGVIVLFHA
                     INDQAVIRQIFRAANYTYGPLLGLFAFGILTKRNVIDKSVPVIGILSPVICYIIDVNS
                     VQWLGGYKFGNELIILNGGITFMGLLLLTKHKTAAQE"
     gene            11920..13350
                     /locus_tag="PKU82_04660"
     CDS             11920..13350
                     /locus_tag="PKU82_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ82200.1"
                     /translation="MENIQQKNSSTSYNVVTNEILEKLKAICGEKYVVNDDESCATYG
                     SDETEDLFFKPEVIVKPSDANEISAILKLANSHFIPVTPRGAGTGLSGGALPVHGGII
                     LSTERLNKIIDIDEKNLQCTVETGVINQHLRDAVEAKGLFYPPDPASRGSCFIGGNLA
                     ECSGGPKAVKYGVTKDYVLNCEVVLPTGEIIWTGANVLKNSTGYNLTQLMIGSEGTLG
                     VITKVVLKLIPLPTETLLMLIPFRSAEKACEAVSAVFRAGITPSAMEFMERDAIEFVM
                     KFTDVKVPLKEDHVAHLLIEVDGNNKDVLFKDCEKISEVMYQHDCDEVMFAESAQQKA
                     DLWRARRAVGEAVKSHSVYKEEDTVVPRASLPVLLAGVKKIGSKYGFRSVCYGHAGDG
                     NLHVNIIKDGMTDEEWNRKLPLGIQEIFELCVSLNGTISGEHGIGWVQKNYMSIRFSD
                     VEIALQKGIKKLFDPNNILNPSKIFA"
     gene            complement(13351..15666)
                     /locus_tag="PKU82_04665"
     CDS             complement(13351..15666)
                     /locus_tag="PKU82_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOZ82201.1"
                     /translation="MNNYLDELNPSQRDAVECMKGPSMIIAGAGSGKTRVLTYRIIHM
                     INNQVDPFNILSLTFTNKAAREMKKRIMEKVGHDAQNLWMGTFHSVFARILRIEAEKI
                     GFPTNFTIYDSDDSKSLIKKIINEQNLDDKTYKPSLVLNRISSAKNNLFSVSDYLNHS
                     ELTNEDKMAGKPKIGMLYEIYQKRLKQSDAMDFDDLLFNTYLLLKNHPEALSKYQNKF
                     KYVMVDEFQDTNYAQYIIVRKLAAAHENICVVGDDAQSIYAFRGANIQNILNFEKDYP
                     DLKTFKLEQNYRSTKTIVGAANSVIANNRNQLKKEVWTENTGGEKIRLLRALSDNEEG
                     AQVAQAIFEDKMNLHFKNKEIAILYRTNAQSRSLEEALRKLNIPYRIFGGLSFYKRKE
                     IKDLLAYFRLTINPYDEEALDRIYNYPTRGIGKTTYEKLLVLADARTCTLWDVMQDIN
                     DREIDIATGTKTKINEFVTMMQSFSVLAKTQNAYDVASHIAQSSGLLKELFNDKTAEG
                     VSQYEHIQELLAGIKEFSDGAQTALEAPSAELEQSETKLLNEYMQDIALLTDAETDDG
                     NDDKVSLMTIHQAKGLEFKEVFVSGLEENLFPSQLAVNSRDELEEERRLFYVAITRAE
                     KRLTLSYAMTRYRWGTLTGCEPSRFLEEINPEFLDALPVSDTKMSPTAIRQGNGVPSF
                     SPNSFTKNNLKPVFKKPVAKQTDPKLFENFIPDDPKRIEIGMSVLHQRFGKGKVMQLE
                     GNLPDLKATVNFEGEGQKQLLLKYARLKIVE"
     gene            complement(15782..16165)
                     /locus_tag="PKU82_04670"
     CDS             complement(15782..16165)
                     /locus_tag="PKU82_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82202.1"
                     /translation="MITKIEMNHVELLEWLKALDFYKNELRFWRKHLSHVVVSNTNKD
                     ILREAEHFQNQVIIQVDQIDLLHHDVKQFENKLDEEFESVSKGGKFSQQIVDVEASLR
                     ERMKTFEHIYVDLKHDYYSFLRKYM"
     gene            16291..16803
                     /locus_tag="PKU82_04675"
     CDS             16291..16803
                     /locus_tag="PKU82_04675"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOZ82203.1"
                     /translation="MMKSFIKYGIMGSLLAFAWTLVGYILGNETQEKLKWLSMLVMII
                     IVVFCFRAVINEEKKANEGFISFGKAFGMAFKTGLVMALCSAVFTYLYFNFINPEYME
                     FAIQKAQEQMQSQGLSDEQIDMSMKMQAKFMTPAMMTIVAFFSSVIIYVIVSLIMGAI
                     MKKENPNEMS"
     gene            complement(16800..18026)
                     /gene="rocD"
                     /locus_tag="PKU82_04680"
     CDS             complement(16800..18026)
                     /gene="rocD"
                     /locus_tag="PKU82_04680"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOZ82204.1"
                     /translation="MSKETTVGSSYFIELEERYGAHNYHPLPVVLSRGEGIFVWDTEG
                     KQYFDFLSSYSAVNQGHCHPRIVASLVEQAGKLALTSRAFYNDKLGEYEKYATELFGY
                     DKLLPMNSGAEAVETAIKLCRKWAYKNKDIARNQAKIIFCEGNFHGRTISIISASTDP
                     TASTNYGPFTPGYVIIPYNNLAALEDALSDPNVAGFIVEPIQGEAGVVVPDDGYLSKA
                     AALCRQNNVLFIADEIQTGLARTGKMLACDYENVKPDILILGKALSGGMLPVSAVLAN
                     DNIMLAIQPGEHGSTYGGNPLACVVATTALQVILDEKLAENATKLGKILRDELNKFKH
                     EMLTLVRGKGLMNAIVIKPSKGKDAWDVCLKLAANGILAKPTHGDIIRFTPPLVMTEE
                     QLYECIAIIKKTLSEF"
     gene            18191..19174
                     /locus_tag="PKU82_04685"
     CDS             18191..19174
                     /locus_tag="PKU82_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOZ82205.1"
                     /translation="MKRKTFSIALLFCIISLLVYGWGFFGHKRINRMAVFTLPAQLMR
                     FYKANIDFIEEHAVDPDKRRYAVKDEAARHYIDLDRYGESPLDSLPKNWKAAVEKYSK
                     DTLMAHGIVPWHINVMLGRLTDAFRNKDYNKILRYSADLGHYVADAHVPLHCTQNYNG
                     QLTNQVGIHGLWESRLPELFADDYDYFIGKAQVIEKPMDYIWEIISQSFAAKDSVLLF
                     EKELNARFDADRKYAFEQRGTTMMRVYSVDYSKQYQDMLNGMVERRMRQAIIAVGSFW
                     LTAWTNAGQPNLDGIEKITISEEMKREIENEDKMWRTGKVLSPGGHADDDH"
     gene            complement(19268..19800)
                     /locus_tag="PKU82_04690"
                     /pseudo
     CDS             complement(19268..19800)
                     /locus_tag="PKU82_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6128 a         3722 c         3700 g         6450 t
ORIGIN      
        1 tatgctaaaa tggctaaggc atttacgcgc ataggaaaag aaattgctat tgctgttcgt
       61 cagggtggcc ccgacccaca ctacaaccct aaattgcgtc aggtaataca gaatgcaaaa
      121 ggtctaaaca tgcccaagga tagggtagat gctgctataa aacgtgctac cagcaaggac
      181 atgaaagact atgaagaaat tacgtatgaa ggttatgctc cctttggcgt tgcaattatg
      241 gtagaatgtg ctaccgataa ttcaaccaga acagttgcta acatacgcat gcacttcaat
      301 cgcgaagagg gaagtttagg cacttcaggc tctgtaggat ttatgtttga aagacgtggt
      361 ttgtttaagt tctctgctga aggaaaaaat attgaagacc tcgaacttga acttatagac
      421 ttcggtttga ttgagatttg ggaagaagac ggattcattt atctctattg caactttgaa
      481 gattttggtg caatgacaaa ggttcttgaa gaccgctcta ttgaaatcat caacagcgaa
      541 cttacacgtg ttccattaac acttaccgaa gtaactgaag agcaggaaca taaaattcaa
      601 gtgcttattg acaggctcga agatgacgaa gatgtgcaaa gtgtttttca caatatgaaa
      661 taaattaaga catcaaaaca gatgtaatta tcattggagc agggcctgca ggttctgcct
      721 gtgtaatggc acttaaagat gccggcctca atattgtctg gattgataaa gccacttttc
      781 cacgtgacaa agtttgtggt gatgccattc cttccaatgt gcaacgcgtg ttaaagacca
      841 ttgatgtttc tctcaacgaa aaatttttac aacaatttat agaaaaagtt caggtagaag
      901 gttgccgttt tgttgcacct gataaatctt cgttcgacct tacatttgtt accaaaggtc
      961 atgctgccaa gcgcttacat ttcgatcatt ttctttttga catggcaaat gaatgtaaca
     1021 gtcatgttac ttttattggc aatgcatctg ttgaattaat cgtaaacagc gcaactgccg
     1081 tcaccgttaa actaactgat ggaaggacaa ttgaaggtca gataattatt ggctgcgatg
     1141 gtgcaaattc catagtcaga aagaatcttg ctcaaacatt cattcgtaaa aaagaaaata
     1201 ttgcagctgt tcgtgcatac tataataatg ttgcagacct tgagcataat atgctcgaga
     1261 ttcatattgt caaaggttat atgcctggat atttctgggt ttttcctctg ccggaaaatc
     1321 aatccaatgt tggctttggc atggttaacc ggtttatcac aaaccgtaaa atagatttaa
     1381 aagaggcttt aaaagaaatt attcaaaacg ataaggcgat tagccctcgc tttagtcagg
     1441 caactactga tggtgtgatt tctggattcg ggcttccatg tggtggacga aaaaacccaa
     1501 tttccggaca aagattttta ctctgtggtg atgcagcttc actcatcaat cctgcaacag
     1561 gtgaaggtat aggcaatgca atgataagtg gacgaatagc tgctttacat atcatcaatt
     1621 gttttcaggc gaacaacttt acgcaatcgt tcaatgcaca atatgatgtg ttggtatatg
     1681 ataaactcct tggcgattta cgtattcaac attggctgca acgcattact gcagacagag
     1741 agtggttaat aaattttgca ttgaagcatg tcagcaacaa taactggata aggcaaaaag
     1801 ttagaaaatt cttctgaaaa ttacgcttcc ttttctttta ttctgataac ctctgcaagc
     1861 gttggtatca aggttttgtg tttcttaaca acaggatttt ctgtatccca aacatatcct
     1921 gccagaacag aacaaatttg cttttttata ttcgggtcaa tatcgtttga gtaaattatg
     1981 gtctgtaagt cgccattata ccaacccaat acaaatgttc tgaaaacgtc tatgcctctg
     2041 cgcaattttt cttcgtaaac ttcagaccaa ttgacttgct cgccattcaa taccttttct
     2101 gtcagtttag ctgctagtaa accggagcct gttgcaagcg ttacgcctga tgaaaataca
     2161 ggatctaaaa attctgtgct gttaccacaa agaacatatc ctggaccaaa caactgtttt
     2221 gaacgcacag cataattatg aattgtgcgt ggctcaaaaa ccaactgttc gttttcaaat
     2281 cgtcctgcca ggtcaccaaa tgttctgata tgattcaaaa acttctcacc attatttgca
     2341 gcaaactcat caaccagttc aacattgctt actacgccta cagaggtagt tttatcgctg
     2401 aaaggaatag cccaaatcca ggcactgttt tcattaaatg aatgaataaa tatatttgca
     2461 cctgcttttt ttgttcgttt ggtgtcatga atgtgacaaa aaacagcacc acggggtgga
     2521 gtactcacag gctcttccaa tttaaataga cgtggtagca cacgaccata tccactggca
     2581 tcaataatgt atttcgactt tacagtatgt gtctttccgg atttatctgt ataggttgtt
     2641 gtttgttcac ttgcagaagc agcaacagaa tttacttcac attcaaattt tacttccaca
     2701 ccttgttcta tagcagttga aattagtatg ttatcaaagt cagcgcgttt tacctgccat
     2761 gtccacgacc atccatcgct aaactgatcg ttaaagaaaa actctgtctt atgttttcct
     2821 ttataaaatg cagcacctgt tttttcctga aatccatgtt tcttgatatt gtcaataaag
     2881 cctacttcag atagatgatc catacaatga ggcagtaaac tttcccctat tacaaaacgt
     2941 ggaaattttt gcttttccag cacaagcact tttatgtttt gtttattcag ataggcagag
     3001 gcaacagcac cggaaggtcc tgcaccaatt atcagtacat ctacattctc catttaatgg
     3061 ttgttgaaat ttttattgcg aagcaaattt aatagtaaac ctaatttgac ttttatgttt
     3121 gaatatgatt tttttcagga tttatctttt ttaacagttc ttttttgtca gtaaattcaa
     3181 agcatgttag caaagcactt attgcagtac ctaaaatgcc atgaaaaact atattttgtc
     3241 ctgttagcca aagattagga atttttgttc ttggttgtaa catacttttc aaaggagcat
     3301 tgctgtcttt taaaactccg taaatcgaac catcagtgct ggcagtataa tctttaaaag
     3361 taagaggtgt tgaactgtaa acattcagta tagcctttct tatttccgga aaaagtgttt
     3421 ctaatttttc aagaactttt tcttcttttc ttcttttaaa ttcctgatag ctttcgctgc
     3481 ggtttgatgg atgtgttgtg gtattaaaac tatctttcca ttgcgcaaca tcatcagatt
     3541 tcatatagca catcacagaa atggattcag catactgcgg gtcttttaat ggaaccggtg
     3601 taaacacaaa gaaacttaat ggccaattgt cttctgtgta attaatagtg tcccaaacat
     3661 catcattaaa aaaatgatac atgttgtagt tcatgtatgg aaatgatttt ggtttaaaag
     3721 taatttgtac taagaatgca gaaacagttt gatccatttg ttttaccctg ttaacccagg
     3781 catgagaaaa gcggtcattg ccgagtagcg aaatagtttc gttggggtgt atggcagaga
     3841 tatattttaa tgctgtaata cactccccat tttctaatag caactgagaa atttcccgat
     3901 tctccttata tgtagctcct gtaatctgtt tatgagtata aatatcagca ccggctttgc
     3961 gaatattact tgcaaactgt ttaattaatt gtgcaccacc ggtgacaatc ttgtagcttc
     4021 cacaaatgta actgttaaaa ataagtgcat gaacaaacat tggtgtcttc tcctttatgc
     4081 ctgcatatag catgttattg cctgcaagta catttctgag cgttttattt tctgtaatgg
     4141 aagcaatata atcccatgca ttttcatttc taagttcctc gttaaaataa tcgtcagtta
     4201 ttttattact cagatcatag agtggaaatt tttggcagta ttctttcaat ttatcacaaa
     4261 accgatgaat ggccttctcc tcatcaggaa aatctttaca caaagaatta atgaaattat
     4321 catatcccat ggcatgagca tattttctgc catcactgaa actgacatag tcataaccgt
     4381 tatcatccat cctttttgtc tgcagtgtgt ctttcaaacc aaaatagctg aataactgat
     4441 agagattttc tccttcatcg tagccgccaa tataatgcag gccggtatct aaaatttttt
     4501 tatctctgct gaaaatctga aggcttcctc ctaattgaat atttttttcc accaaggcta
     4561 ctttataacc ttctttagct aagatataag cacaacacat tcctgaaaaa ccaccaccaa
     4621 ttatggctat atcataaact attttgtcat tgattttcat ttgctcagta caaatagtcg
     4681 gttggaagta ttattggaat gtttatgttc ttcaattttc aggttgtttc tttttgcaaa
     4741 ttgataaata tactcagttt tgaaaaaatt taagtcattc attttcttat tgaaggaaaa
     4801 gaattttgta gataataact cagtaaattt agtgtttcta tgtttaatat catcagcaac
     4861 accatcacga ataaacatta ttccgccagg catcagggca tctgcacatt tctgcagcac
     4921 tttttcctga ctgtcaatat ttaggtaatg taatacgtca ttaagaaaaa ttacctcaga
     4981 tgagggtact tctgtttttg ttatgtcggc agcaaagaat tgtaaatttg cattcttatt
     5041 ccagacattg gcagcaatgg caattttatc atcatcataa tcaaaagccg atatctttct
     5101 ttcacgattt ttgtaatgca gaaacaagtc cataaatcca taaccgcagc ctaagtctgt
     5161 gattaatatt cgctgaccaa taaggttatt ataaaaatcg taattctttt tttcgagatt
     5221 ccattttatc cgaccatacc attctaaaac cgggccttta aaaatatagt tgtaataaac
     5281 tttttgccat aaatagtctg catcttcgcg ttcatcttta aaaagtctgt actgctcttt
     5341 aaaatattgt gaaatggatt tttgcctgct tctgtaatcg ttaccccaac tcaaatcatt
     5401 tgcaaatatt cttggtaaag cttttacatt aatggtgcca tctttaacca tcatttcatt
     5461 tttgggtgaa acatcgcctg caccatgaat catcacgggt tgaatatcag cattcagttt
     5521 ttctgcaaga taaaaggctc ctttatgaaa gcgattaaga tttccatcag atgaacgcga
     5581 gccttccgga aaaatcataa tggaataacc ttcatccatc ttagctttaa tgttggttat
     5641 attatcttca gttccatctt cagaaaaaat ataaccgaca tagcgaacca ccaaaccgaa
     5701 tagaggagaa cggtaaaccc aatcttttgc catgataata acttttctgt ttaacattag
     5761 cataatcata atatcgagaa aagaagagtg gttggcaata ataattgaag gctttttgaa
     5821 atcaagttga tcaaggttat aaaatttctt ttttacatga atgccagaat acagtacgga
     5881 tgctgaaaat tttgatacaa taaagtttac tgcttttctt ttaattttta caggtgcagg
     5941 gaataggctg atgagaagca aaacaggata gaaagtaagg caaccaacaa taaaataggt
     6001 gaaacaaaaa acggacataa aaagttgtag aaatgaaatt ggattgtgtc catttttaat
     6061 tctgcgttct acaaaaagtt taaaaataaa tggttgaaca agaaatgaaa tcagtaagat
     6121 gcaggtgatg ccaataacac ttaatgcagc aatggaatga attgcagggt gcttagcaaa
     6181 atataataca ccggtgccca caataactgt caatgcagac aggatgattg cagcctgata
     6241 ggattttaga ttgtctttcc tgaatttata acggttcaaa agtccatcag taacgaatat
     6301 actaaagtca tcaccgagac caaaaatgaa agttgtaatt acaatattta caaagttaaa
     6361 ttcaatatta aaggtggacg acattcctaa tatccaaatc caactaacaa ccattggcaa
     6421 aaatgcaaga agggcaagct caaccctgcc gtaaataagc aataaagtaa aaaacaccat
     6481 gcctgctgtg aggtacagaa ggtagttgaa atcgctgcgc accgactcaa gcagcagtga
     6541 tgccatttca cttctgtcaa atacggtaag atgcgggata tctttaaata aagctttcac
     6601 agagtctttc tgtgatgttg ccactgtaaa agatgaaata aaaacagatt cttttgcact
     6661 tgaatcaaac attaggtcta agcccattgt tttgagtaag tcttttgaca atggttgata
     6721 gatgctatcg tcaatcattt cttgaaatgg agcaaaagca ttgttgctga agcctgcttc
     6781 atcggcagat agttttatct gattggtgac acgggtttta ttctgttgcc agaagtaatt
     6841 ccatcttagc tgacgttgtt tacaaagaga atccggaata aaataatcag ctgtagataa
     6901 agtgtttttt atgatgccat gtgttattaa attctttatt aatgttcctg ctttgtaatt
     6961 tgcatcaact gcagagttat aatttttagc tgaagcaatc aggtaaatct ttttttcaat
     7021 agacggattt atgcctgtaa gttcagcttc tttatttttc aactcagtag gatgaaagct
     7081 taaagtttca acatcgctgt tgaaacgaat gtttgatgct tcaaaataaa aatatacagt
     7141 tattagtaaa atggcaataa ttgcatattt aactattgat ggattaatga ccggcagttt
     7201 aaaccatttt attgtcttat gctgtgtgta atcaaagcgg gtcaaagtaa ttattacagg
     7261 taggcctgca agagtaaaaa aagccgaacc agccaaagcc attgctgcaa acaagccaaa
     7321 atcacccaat acaacagagt tggtataatt taatgcaagc aaagccagca ctgttgtcac
     7381 acttccggta agcaacggta cagaaatttc ttttaaagtt tcttcaatag attttacatg
     7441 ttgtagatga gtgaagaaat gaaaggaata gtccaggatg attccaatta cgatggcgcc
     7501 tgttgctaat gatatggctg aaatttcagg gcgaagaaaa cccatcagtc caagtgctaa
     7561 catgcctcca aacactgcag gtagaataaa taatattgga atacttagtt ttctgtaata
     7621 caaaatcaga atgagcatga ttagtgataa tgaaagaatc atggtcaggt tggtgtcctt
     7681 ctttatctgt actgcatttt gtgctgcaat ttcaaaggtg ccgaaatgtg ataatttgta
     7741 ttcagagttt tcagcattcc attttttctc tatagcagca agttggtcac taagttggat
     7801 gtttttttca gcatcacgac tatcatagtt cagacggcct gtaataagaa tagacttttt
     7861 gcccggtgta aaaatgatac catcttccac aacataattg ctactatttc ctgtaatgcc
     7921 aagttgtcta aaatattctg cagtgatgct taatggatcg ttaagaataa attgtttgat
     7981 aaataaaccg gatggattgg taagtctttc gtaattgttt ttaatcgaaa attgtataga
     8041 atcatcctgt atttttcttt caatcaaaga ataataatta ctatcaatca gcagagggaa
     8101 gtgcgagaaa taatagtcgt aaacttcttg ttgcacatca ggtcttactg caacaacatc
     8161 actcaataaa ttagctgaag ccgaatctat agtacttttt acatgttcgg ccacatcggc
     8221 atattcatca gggtttgagt ttttcgttaa tgataaagaa aacacaacct gatcagagat
     8281 gttttttgat tcaagaagag aattgaattt ctgaaactcc tgaccttttg gaagaatagc
     8341 aaatatattt tcagtaatgt ttagtctttt aattcccgca atactaacag cagcaaccag
     8401 caaaacaaag cctgcaaaca gtataggctt tcttacaaac agacgatata atccaataaa
     8461 aatacttccc aagaagaacc gattttacaa cgtaactttc agtgtgcgaa tttataggaa
     8521 ttaatgtgtc aaactttcat aaaacgttta tatactttca tttttaaaat gattactttt
     8581 gcctctcatt ctaatattaa atcaaaatgg ctgaagattt tcttccgtta aacggaaccg
     8641 accacgtaga actttatgta ggcaatgcca agcaatctgc atattattat cagatggcat
     8701 ggggctatga acttatagct tatgccggcc cagaaaccgg tattcgtgac agagcatcgt
     8761 atgtattgca acaaggcaaa atccgtttgg tgctgaccag tgccatgcat cctgacagtg
     8821 agattacacg acacgtagcg cagcatggtg atggtgttaa gtttttagct atttgggttg
     8881 acgatgctga aaagtctttt tatgaaacta tgagtcgtgg tgcaaaggcg cacacacagc
     8941 ctacggtttt gagtgacaaa tttggtgaag tgaaaatttc tgctattcat acttatggcg
     9001 aaactgtaca taagttcgta gaacgaaaaa attataatgg ggcctttttg cccggatatt
     9061 caccggccaa aagcagtgta aaaactactc ctgtcgggtt aaagcatgtt gatcattgcg
     9121 tgggcaatgt tgagttgggg agaatgaatg aatgggttaa gttttatgaa gatgtcatgg
     9181 gatttaagtt gctgattacc tttgatgaca gcgatatttc tacagaatat tctgctttaa
     9241 tgagtaaggt tgtcagcaat ggaaacggtt acattaaatt cccgattaac gaacctgctg
     9301 acggtaaacg caaatctcaa attgaagaat acattgaatt ttatcatggt gccggagtgc
     9361 agcacgttgc catagccact gatgatataa tttcaacggt aactgagttg cgcaacaggg
     9421 gagtagagtt cttgcatgtt ccggaagttt actatgaaga tgtttgggat cgtgttggaa
     9481 aaattaacga agatgtagct acaattaaaa aactgaatat tttagttgat cgtgatgatg
     9541 agggctactt gcttcagttg ttcaccaagc ctgttcagga cagaccaaca gtattttttg
     9601 aaatcattca gcgttgcggt gctaaatctt tcggaaaagg aaacttcaaa gcgctttttg
     9661 aagctataga gcgcgagcaa gcactgagag gaaacttgta atgacgtact gcctatgatg
     9721 gcagttattt taccaaagtg ttttcgtagg ctacacggtt aattaacgaa cggccgagtg
     9781 taacttcatc ggcaaattcc aattcgccac caatggctac acctcgtgca agtattgaaa
     9841 ttttaacatc aatgtccttt aatctttttg agatataaaa cacagttgtg tcaccttcca
     9901 tggtagcact gagtgccatt attatttcgt ttatatttcc attagcagtt ttttcaacca
     9961 acgaatcaat attcagttgc gaagggccaa tgccatccat gggagaaata atgccgccaa
    10021 gaacatgata catgccatta aattgtgctg tgttttcaat tgccattaca tcacgaatat
    10081 cctgtacaac acaaattaca ctgccgtcac gtcttggatt tgcacagatt gcacataaag
    10141 tgttttcaga aatattatga cattgatggc agtaaactac cgatgaacgt aattgtatta
    10201 cagcattgcc caaacgctcc acatcagcag gattttgttt gagcaaatgc aatgcaaggc
    10261 gcaatgcacc tttacggcca attcccggca gacgcgataa ttcgttaact gcttcttcta
    10321 ataaacgtga cggatactgc tccacctaat gaaaatttaa atgatgttgt caaatatacg
    10381 ttgtttaaaa ataccatctt tcatgcgctg tcaaaggttt tcaaacagtt attcttgtat
    10441 tctaaatttc aaacaattga gtcctgtctt aattttatca gtggttttgg tttataccat
    10501 tctgctgttt gcaattacat tttacacttc gcgcaatgcc gacaatcaga gttattttgt
    10561 tggtaacaga agttcgcgtt ggtatgtggt tgcatatgga atgatcgggg catcgttatc
    10621 aggtgttaca tttatgtcag tacccggtga tgttggtaca ggaaatttct cttactttca
    10681 aatggtgctt ggctatttgt taggctatgc tgtcattgct tttgtgttgc tacctgttta
    10741 ttaccgtatg aatctgactt caatctatac ttatctgaag tcgaggcttg gagaaaaaac
    10801 gtataagaca ggtgcattct tctttattct ttcacgtgta gttggtgctt cgttcagaat
    10861 gtatattgtg gtatatgtgt tgcagctttt tatttttcag gcttggggcg ttccgttttg
    10921 ggtcaccgta tttgtgtttg tcctgctgat actctcatac acattcaaag gtggtgtaaa
    10981 aacaattgtg tggacagatt cattgcagac aacttttatg ctgctttcgc tctttatttc
    11041 ggtttatctt attgccaaag agcttaattt tagttttgct gatgtagtat catcaatcaa
    11101 atcaagccca tactcagata tttttgtgtg ggactggagg tcgaaagtgt tttttccgaa
    11161 acattttttt ggaggaatgt ttattgccat tgccatgacg gggcttgatc aggaaatgat
    11221 gcagaaaaat atcagttgca gaaatgtacg cgaatcgcaa aaaaatatga tgacattcag
    11281 catcatttta gtttttgtaa attttatttt tctctgtctt ggagcattgt tgtatatttt
    11341 tattcagcat cagggcattg ttattcctga gcgcacaaca gatgatttgt tcccgacaat
    11401 tgccatgcat tatctcggca ctttttcagc tatttgtttt atcattggat tgatatctgc
    11461 tgcttatcca agtgcagatg gagcattaac agcactaaca gcatcggtgt gtattgattt
    11521 tcttggcttt gaagaaggta aatatacaga ggcgcaaaaa acaaaaatca gaaaaagtgt
    11581 acatgtcatt tttggttttg tattacttgg tgttatcgtt ttgtttcatg ccatcaacga
    11641 tcaggctgtg atacgtcaga ttttcagagc agcaaattat acctatggtc ccttgcttgg
    11701 actttttgca ttcggaattc taactaaaag aaatgtaatt gataaatcag tgcctgtaat
    11761 tgggatatta tcgcccgtaa tttgttatat cattgatgta aactctgtgc agtggcttgg
    11821 tggatataaa ttcgggaatg aattaataat tcttaatggc ggcatcacct ttatgggctt
    11881 acttttgtta accaaacata aaaccgcagc acaagagtaa tggaaaatat acagcagaaa
    11941 aatagtagca catcatataa tgttgttacg aatgaaattc ttgaaaaact aaaagctatt
    12001 tgtggcgaaa aatatgttgt caacgatgat gaatcatgtg caacctatgg cagtgacgaa
    12061 actgaagatt tgttttttaa accggaagta atagtaaaac cgtctgacgc caatgagatt
    12121 tcagcaatat tgaaacttgc caattcacat tttattccag tcacaccacg tggtgcaggc
    12181 accggtttaa gtggtggtgc cttgcctgtg catggcggaa ttattttgtc cacagagcga
    12241 ctaaataaaa ttattgacat tgatgaaaag aaccttcaat gcacggtcga aacaggtgta
    12301 atcaatcagc atttgcgcga tgcagtagaa gccaaaggct tattttatcc tcctgaccct
    12361 gccagcagag gaagttgttt tattggcggt aatcttgccg agtgtagcgg tggacctaaa
    12421 gctgtaaagt atggtgttac taaagattat gtactgaatt gcgaagtagt gcttccaacg
    12481 ggagaaataa tatggacagg tgccaatgtg ctgaaaaatt ccacaggata taatctgaca
    12541 cagttgatga taggtagtga aggtacatta ggtgtcataa caaaagtggt gttaaagtta
    12601 attcccttgc caacagaaac tttgctgatg ctcattcctt ttcgatctgc agaaaaagca
    12661 tgtgaagctg tttcagcggt tttcagagca ggaattacac cttctgcaat ggagtttatg
    12721 gaacgtgatg ccattgaatt tgtgatgaaa tttactgatg taaaagtgcc gcttaaagaa
    12781 gatcatgttg cgcatttact gattgaagtt gacggcaaca acaaagatgt tttatttaaa
    12841 gactgcgaaa aaatttcaga agtcatgtat cagcacgatt gcgatgaagt gatgtttgca
    12901 gaaagtgcac agcaaaaagc agacctctgg cgtgcaagac gagctgtagg cgaagctgta
    12961 aagtcacatt ctgtgtataa ggaggaagat actgttgtgc ctcgtgccag tttacctgta
    13021 ttgcttgcag gagtaaaaaa aattggaagc aaatatggat ttcgcagtgt ttgctatggt
    13081 catgccggtg atggaaatct gcatgtaaac attattaaag atggcatgac tgacgaggag
    13141 tggaatcgta aattaccatt aggtattcag gaaatatttg aactttgtgt atcgttgaat
    13201 ggaaccattt ccggagagca tggaataggg tgggtgcaaa aaaactatat gtctatacgt
    13261 ttttcagatg ttgaaattgc acttcaaaaa ggaattaaaa aactctttga tcccaataat
    13321 attttaaatc cttcaaaaat ttttgcctga ttattctaca attttcagtc gtgcatattt
    13381 caacagcaat tgcttttgcc cttcaccttc aaagttcacg gtagctttca aatcaggcaa
    13441 gttgccttcc aactgcatca cttttccttt accaaaccgt tgatgtaata cactcattcc
    13501 aatttcaatt cttttaggat catcaggaat aaagttttca aacaatttgg ggtcggtttg
    13561 tttggcaaca ggctttttaa atacaggttt aagattgttt tttgtaaaac tgttggggct
    13621 gaaacttggc acaccattgc cttgccttat tgcagtaggt gacatctttg tatcactaac
    13681 gggcagtgca tccaagaact caggattaat ttcttcaaga aaacgactag gctcgcaacc
    13741 tgttaatgtt ccccaacgat aacgtgtcat ggcataactt agtgtcagcc gcttttctgc
    13801 tctggtaatg gcaacataaa acaaacgcct ttcttcttca agttcatcac gcgagttaac
    13861 agccaactgc gatgggaata aattttcttc aaggccggaa acaaaaactt ccttaaactc
    13921 aagtccctta gcctgatgaa tggtcatcag tgaaacttta tcatcattac catcatcggt
    13981 ttcggcatct gtaagcaggg caatgtcctg catatattcg ttcagcaatt ttgtttcaga
    14041 ttgctccagt tctgctgatg gtgcttcaag agctgtttgt gcaccatcgc taaactcttt
    14101 tatacctgcc agcaactcct gtatatgttc gtattgactg acaccttctg cagttttatc
    14161 attaaataat tctttcaaca atccgcttga ttgagcaatg tgacttgcca catcataagc
    14221 attttgagtc tttgccaaaa cagaaaaact ttgcatcata gtaacaaact cattgatttt
    14281 tgtttttgtt cccgttgcaa tatcaatctc acgatcgttt atatcctgca tcacatccca
    14341 cagcgtacaa gtacgtgcat cagcaagaac taatagtttt tcataagtgg ttttgccgat
    14401 acctcgggta gggtagttgt aaattctgtc aagagcttct tcgtcatacg gattaattgt
    14461 aagtcgaaaa taagcgagta aatcttttat ttcttttcgt ttataaaaag acaagcctcc
    14521 gaaaattctg taaggaatat ttagtttacg caatgcttct tctaaacttc tagactgtgc
    14581 attggtgcga taaagaattg caatttcttt gtttttaaaa tgcagattca ttttgtcttc
    14641 aaagattgcc tgtgctacct gtgcaccttc ctcattgtcg cttaatgccc tgagtaatct
    14701 tattttttcg ccacctgtat tttctgtcca gacttctttt ttaagttgat ttctgttgtt
    14761 ggcaattaca ctgtttgctg cgcctactat ggtctttgtg cttctgtaat tctgttctaa
    14821 tttaaaagtc tttaaatcag gatagtcttt ctcgaagttg agaatatttt gaatgttagc
    14881 acctcggaat gcataaatac tttgtgcatc atcacccaca acacaaatat tttcatgtgc
    14941 tgctgcaagt tttctgacaa taatgtattg tgcatagttt gtgtcctgaa actcatcaac
    15001 catcacatac ttaaatttat tctgatactt cgacaacgct tcagggtgat ttttaaggag
    15061 caaataggta ttgaataaca aatcatcaaa atccatggca tcagactgtt tcaatcgctt
    15121 ttgataaatt tcgtatagca tacctatttt tggtttgcct gccattttat cttcattggt
    15181 caactcggag tgattcagat agtcacttac agaaaacaaa ttattttttg cagacgaaat
    15241 tctgttcagt accaatgaag gcttgtaggt tttgtcatca agattctgtt cattgataat
    15301 ttttttaatc agactttttg aatcatcaga atcataaatg gtgaaatttg tagggaatcc
    15361 tattttctcg gcttcaattc ttaaaatacg tgcaaatact gagtgaaagg ttcccatcca
    15421 caaattttga gcatcatgac caactttttc catgatgcgc tttttcattt cacgggctgc
    15481 tttattggta aaggttaaag acaaaatatt aaatgggtca acctgattgt tgatcatgtg
    15541 tatgattctg taggtaagca ccctggtttt tcccgaacct gcaccggcaa taatcatact
    15601 aggccctttc atgcactcaa ctgcatcacg ctgcgatgga tttaattcat ctaaataatt
    15661 attcataatg gaatttacta ctcttcaaag ataagggttg ctttggttat taaaatgaaa
    15721 acagggtatg ttttttcata ccctgctatt gaaaactttt taattaattt caaactaatg
    15781 attacatata ttttctgaga aaagaataat aatcatgttt cagatcaaca taaatatgct
    15841 caaatgtttt catccgttcg cgaagtgatg cttctacatc aacaatttgc tgcgaaaatt
    15901 ttccgccttt acttactgat tcaaattctt cgtctaattt attttcaaat tgttttacat
    15961 catgatgcag caggtctatc tggtcaacct gaatgataac ctgattctga aaatgttcag
    16021 cttcgcgaag tatatctttg tttgtattgc ttacaactac atgtgacaga tgtttacgcc
    16081 aaaatctcaa ttcattcttg taaaaatcca gtgccttcaa ccattcgagt aattcaacat
    16141 ggttcatttc aatttttgtt atcatacatt atgttctttt gtagctgcta aaatagcaca
    16201 tttccgactt accttagtta ctgtctcggc aataaatttc tttgtatgct ttaaaaaact
    16261 aattttggct tgtaaacatt aaatcaattc atgatgaaat catttatcaa atatggaatt
    16321 atgggctcat tgctcgcatt tgcatggact ttggttggtt acattttagg taacgaaaca
    16381 caggaaaaac tcaaatggct tagcatgttg gtgatgatta taattgtcgt gttttgtttc
    16441 agggcagtta tcaatgaaga gaaaaaagct aatgaaggtt ttatttcttt tggtaaggct
    16501 tttggcatgg catttaaaac tggtttggta atggctttat gcagtgccgt tttcacttat
    16561 ttatatttta acttcataaa tccggaatac atggagtttg ccattcaaaa ggcacaggag
    16621 caaatgcaat cacaaggttt gtctgacgag cagattgata tgtcaatgaa aatgcaggct
    16681 aaatttatga caccggcaat gatgacaata gttgcatttt tcagctcggt aattatttat
    16741 gtaattgtat ctttgataat gggtgcaata atgaaaaaag agaatcctaa tgagatgagt
    16801 taaaattcac tcaacgtttt cttaataatt gcaatacatt cataaagctg ttcttctgtc
    16861 atcaccaatg gtggtgtaaa tctgataata tctccatgag tgggcttggc caaaatgcca
    16921 tttgctgcta atttcaaaca aacatcccat gcatcttttc ctttgctagg ttttatcaca
    16981 atagcattca tcagcccttt gccgcgaacc aatgtcagca tttcatgctt aaatttattt
    17041 aactcatcac gcagaatttt acccaattta gttgcattct ctgcaagttt ttcatcaagt
    17101 attacctgta aggctgttgt tgcaactaca caggccaatg ggtttcctcc ataggttgaa
    17161 ccatgttctc ccggctgaat cgctaacata atattatcat tagctaaaac agcactcacc
    17221 ggcaacatgc caccactcaa tgcttttccc aaaatcaaaa tatcgggttt cacgttctca
    17281 taatcgcagg caagcatttt acctgtacga gctaaccctg tttgaatttc atcagcaata
    17341 aacaaaacat tattttgcct gcataatgct gctgctttag acaagtagcc atcatcagga
    17401 acaacaacac cggcttcgcc ctgtataggc tcaacaataa atcctgcaac gttagggtcg
    17461 gagagagcat cttccaacgc tgccagatta ttatatggaa taattacata tcccggagta
    17521 aacgggccat agtttgttga tgctgttggg tctgtagatg cagaaataat tgaaatagta
    17581 cgtccgtgaa agtttccttc acaaaaaata atttttgcct gattgcgtgc aatatcttta
    17641 ttcttataag cccactttct gcaaagctta atggctgttt caacagcttc tgcgcctgaa
    17701 ttcatcggca acagtttatc atagccaaac aactcagttg catacttttc gtattctcca
    17761 agcttgtcat tataaaaagc tcttgacgta agtgctaact ttcctgcctg ctcaactaac
    17821 gatgccacaa tacgcggatg acaatgaccc tgatttacgg cactgtatga cgataaaaaa
    17881 tcaaaatact gtttgccttc cgtatcccaa acaaaaatac cttcgccacg gctcaacact
    17941 accggcaacg gatgatagtt atgtgcacca taacgctctt ccagttcaat aaaataactt
    18001 gatcctacag ttgtttcctt actcatttcc ataataaaaa attgtctgat gcaaatttaa
    18061 tcaattgccg ccactttata ggtaggcatt agaatattac gctgtcaaaa cattcaaaca
    18121 atcaatatca cttcagtcaa acatatcaaa tcaaattaaa acaactattt ttaacgcttc
    18181 aacaatttac atgaaaagaa aaactttttc tattgccttg cttttttgca tcatcagtct
    18241 gttggtttat ggttggggat tttttggtca taaacgaatt aacagaatgg ctgtctttac
    18301 tttgccagct caactaatgc gtttctacaa agccaacatt gattttattg aagaacatgc
    18361 agttgatccc gacaaaagac gctatgccgt aaaagacgaa gccgcgcgtc actacattga
    18421 cctcgaccgc tatggcgaat ctcctctcga cagcctgcct aaaaactgga aggctgctgt
    18481 cgaaaaatac agcaaagaca ccctcatggc gcatggcatt gtgccttggc atatcaacgt
    18541 catgcttggc cgcctcacag atgcattccg aaacaaagac tacaacaaaa ttctacgcta
    18601 ctctgccgac cttggacact atgttgccga tgcacatgtg ccgctgcact gcacacaaaa
    18661 ctataacgga caactcacca atcaggtggg cattcatggc ttatgggagt cgcgcctgcc
    18721 cgaactcttt gccgatgatt acgactactt cattggcaaa gcacaggtca tcgaaaaacc
    18781 tatggactac atctgggaaa tcatcagtca aagttttgct gcaaaagatt ctgttctgtt
    18841 attcgaaaaa gaactcaatg cccgattcga tgccgaccgc aaatatgcct ttgagcagcg
    18901 tggaaccacc atgatgcgtg tctattcagt tgactattcc aaacaatatc aggacatgct
    18961 caacggcatg gtggaacgca gaatgcgaca ggcaattatt gcagtaggca gcttctggct
    19021 cacagcatgg accaatgccg gacagcctaa cctcgatggt atcgaaaaaa ttaccatcag
    19081 cgaggagatg aaacgcgaaa ttgaaaacga agacaaaatg tggcgcacgg gcaaagtgct
    19141 gtcaccgggt ggacatgcag atgatgatca ttgatttttt ggagtatcaa ccaatagttt
    19201 tttatttttt cttgaagcac aaactgcagt aacatctgca aacgtttcta ccgataacta
    19261 tcggacttca tagttcagcc agttttaaat cggtaaattg attagcactg ctatatacat
    19321 aaaaatgtgg ctctgtaact attcgtgctt ctacaggatt gttgtgtatg taatttactt
    19381 tttgatcaat aaatttatta ctgaataaat cgaaggcatg attattctgt tgccaaaact
    19441 gatatttaac attgtgcgca tttttttgcc gtaaaactca aacatataaa gcaaccattc
    19501 gcgcctgctt tccgtgtggc tttcttcaat cattttaata atttgctttg atgtgttact
    19561 cttaaagtcg cgtaacacat cgcttaaact cacatcgtct ttcgtttttg caattaaatg
    19621 aatgtgtgat ggcataatgc aaaaagcaaa cacttttagt tttttattgt caatgcagta
    19681 atttaagttc ttaactatct catcgcagta aatggctctt gtaaaaacat caatccaacc
    19741 aactgtggtt aatgtaacaa agtatagccc atcaggaaat attttgtatt tttcgctcat
    19801 gcaccggcaa agtaagtctt atagtttgca agatgcaaac aaaataaatg gcacgagcgg
    19861 aaaggacgct cgcacctacg ggagcataat tagtgataat aaaagacttt gtacaagaca
    19921 tacccaaaca aaagaggctg cccttttggg acagcctctt taatattact taagaaaaat
    19981 tatttcaatt tatctttaag
//
LOCUS       DAOOOL010000036        19883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_207_length_19883_cov_171.316068, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000036 DAOOOL010000000
VERSION     DAOOOL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207_length_19883_cov_171.316068"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2639
                     /locus_tag="PKU82_04695"
     CDS             <1..2639
                     /locus_tag="PKU82_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ82206.1"
                     /translation="FRLMNGDDGNKSWNMQGELRSIAKEIFKDHLKVNFETDVLADTE
                     GINEVKSLYAAIWNKLSGMAKKFLEIADSAGVEPIDFYYGRGGFHSFIVKIAAKVHPK
                     DNTIGQRTWQILDREDVFADNAQDFLKQDVSLNGELLSLVRQIVDYYITNIERLLSAE
                     VVTANIYQAVILKYLFRQLKIYRKDHQILLMSDVGKILNSIIGNNDTPFIYEKAGNKY
                     RNFLIDEFQDTSQVHWKNLLPLLINSLSEQHQVLTVGDGKQSIYRWRGGDMNLLLNEV
                     ESTLKNHANSTDIFRLDSNFRSSVSVVEFNNIFFSAINEIVSADSQQSQLLKKAYQAD
                     VVHQVAKRSEEKGFVSIELYLDKEEYPELNYPEYFSEADKVVASHTLSYIQSQIEKGY
                     KPGDIALLVRTNVKGNMLADFLVLNGYNNINSRDSLLICHAPQIRFLINCLRLNADNK
                     NQVAEKHILYFVSKHLRMETKLIETNIEKYVKHVKLYSLTEFCAQVISKFGLNRQPDA
                     YLQCFEDLIFEKSSKGISTTAEFLDWWDVESNQSKISVQVPADENAITIITIHKSKGL
                     QFPIVIIPYTDWDLNSNKGLIWIRSDISPFDKLGAFPVRPSKKLLKTYFGQDYILENR
                     EKVLEEVNALYVACTRAEDKLFILISGSGEEGEDGINKVNTLIKSAVMNAETVSSTLT
                     KASDTVYTLGVDSEYMQKRKKLSDTPDSFTNKELVNYFTSVNRISTGFKIKKYAEQSK
                     SRLIGIKVHELLADFRSESDLKAIEKHLSNLQLPDDEKNELLNGVQTTCNFLSEKKWV
                     SPHFKIYNEHEMADAEGNILRPDRLMIEGDNAVVIDYKTGAIETSHVQQIANYKEVLL
                     DAGFKDVKTFLYYLEGKELVEA"
     gene            complement(2647..3045)
                     /locus_tag="PKU82_04700"
     CDS             complement(2647..3045)
                     /locus_tag="PKU82_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82207.1"
                     /translation="MKNVHSKSQIIIFDNYGNILKSCDTIFQVSDLIGKNIMKQFPLL
                     NCYEPEIRNLSKSKEPFFVPCIAFSFANFKSICDFVFIRHEELNEITISWMISDNYVH
                     FKERLNKPINLPQTKSKPMVTEFVNRWHGK"
     gene            complement(3047..3562)
                     /locus_tag="PKU82_04705"
     CDS             complement(3047..3562)
                     /locus_tag="PKU82_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOZ82208.1"
                     /translation="MQNKKILITGYSQCGKSSIVNRLVNNRFHHSYSEDSQLCIARKE
                     LAINKKKINFVIWTAPGSVSNLKSFKSYYLGTDALIHVVDVNNPSTFSDLDAWISHYQ
                     KFLPGTPVYLTCNKIESNINLDCSDFFTQFDCYRTFFTSAKNNFMINEMFQTIAADLL
                     NEPFFQKIKIY"
     gene            complement(3634..9447)
                     /locus_tag="PKU82_04710"
     CDS             complement(3634..9447)
                     /locus_tag="PKU82_04710"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82209.1"
                     /translation="MHFTVKLAKINFLVGGNSALNKMSGMWLTALVIIFSLHQSIAQL
                     PVASFGISQSSGCAPLTVSFNNTSTNATSYHWDFGNGAVSVLNSPVITFTTAGNYYVK
                     LIATNSSGSDSVISSQPIHIENKPVVQFTLNKSSACQGEIINFTNQSAIFDSCQWDFG
                     DGVTSNQTNPTHIYATAGTYNVTLVLFRLNSTCTQTLTKNNIIIIKPIPIVTATVDTF
                     STCNINKIFHFNAGANTTSSYQWNFGDGGTATGAIVQHQYSQSGNYQVAVTASASGCS
                     STVNLSSPITVLNNPIPIITSNITSGCNPFNVVLSTNTTGTSSLLWNLDNGQTSTLST
                     VGVLYNNAGNYNPYVTANYPNGCSSQSAPLTINVLQSPVSTFTTSNTSGCKPLSISFQ
                     TTSCTGCSYQWSFGDGNSSTQLNGIHNYTQSGTFYPALTVTAANGCTATSNLPYGITV
                     NGPEADFLADKVTGCNPLTVNFANTSTGSTSWLWQFGTGDTSTVNSPTYLYQNQGSYN
                     VTLIASDGNGCKDTLRKQGYINPGPSVNNFSNPQPVSGCAPLTVSLSDSSGANSWLWD
                     YGDGSTATAQNVSHTYTTPGTYTVSLQTQSNGSTCSQTISNFATYIVGGGIANFTYNQ
                     TLCPPFIGTFTDSSLNAVSWLWNFGDGQTSTQQNPVHTYNQTGSYNVSLTITSANGCT
                     STKVEQYAMNFLTLGAAPSAHTLDTIPPLDVQFSANSVGATSWLWTFGDGDSSTLENP
                     MHTYQSTGPFTISLTIANDSCSYTYSFPPTNFGSGGTFYDENHDTTQFTMHYDGCAPL
                     TISFNNPFLNVVGSYWDFGDGNTSSQQNPVHTYNVPGIYDVMLIANLSNASVDTIYHS
                     QAVKVAGTPVTFIVNTVNTCNGSNIQALPSNLNLQSYNWDFGDNQTSSQVSPIHSYAN
                     TSNYIISLQAQDTSGCTVIHSKSLYANADANAILSNKNRACASDTITFSVQNTNYVNY
                     TWDFGDGTSATGNPIAHTYSDSGSYSVQVVITDINGCTQTVNASQQITIVKSVADFNI
                     NMLSSSCTMVNVQLTNTSTGSTSYTWDLGDGTMSSLTDVNKYYYYGPNPPYTYDIKLT
                     AYNNGCASTKTLAQAVTIPDFDVKFSYSRSQGCLPITINVTDSSLNAVAWKWSWGDGD
                     TTFTQATQHTFTSLPTDKIKLFAKDINGCEKNYELDNIQLPVAEFTQSDTIGCNPKTI
                     QFTDSSKNAHQWLWKFGNGSTSITPNPSINYSSTGNFIPSLIITDSFGCKDTVGGKII
                     SITEPQADFSYTKSSGCVPVIVQFQNTSSGAANYLWDFGDGSTSTLLNPNHIYTTGGN
                     YQVTLTVTDASGCSNTKTLSNALHIPGPLASFSLSNNHGCQPLTISTNDSSQNALSHF
                     WSFGDGDTSVATSPIHTFNTAGMYLITLIVKDANGCQSVYTHPDTIKVYNKPVAAFAI
                     SDSTICVGTSISLLNQSQSSSSYKWQFGDGTVSNQSNTSHQYQIPGQFSITLVASNGH
                     CSDTANSINAVTVIPFPIADFGGSPLIGCAPLSSNMVNISSSLQNPAFIWDGGNGLTS
                     SAFNPQFNYQNAGYYTVTLKVTNEGLCSDSITKLNFIAAQNNQPITPSEINYLTVLDS
                     NAIRISWQPSSATNLNRFELYRSTDDKNYNLIYTDTNTHNSTLNLQLAFIDNNVTTNQ
                     NRYYYKLQTINNCNNSYPLDSSISHASILLTTEPDTMSNMLSWLPYIGKTISQYSIYR
                     STDGLNYDLIDEIAGTEDFYIDSTAYCPQEYSYKVEAQLDSSIYTSESNNDASVPTSF
                     VNQITTSIIRSTVVDNNSILTEWHEGNPFPELVYYYLIYKSSDKDPGNFVLIDSVNNS
                     TKYYLDRDVDVNATNYQYKIEVENICQQRTGLTDLSGNILLQGKLDDMNNSRLEWTPY
                     KGWKEGVERYEIQKQDENGNWKTIKSVDGKTHHMEEE"
     gene            9594..11387
                     /locus_tag="PKU82_04715"
     CDS             9594..11387
                     /locus_tag="PKU82_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ82210.1"
                     /translation="MSAIQEKNQSVKGGEFIIKESSAENTFIPEEFSEEHLMIEQTAN
                     DFLKQEVHTVLDRIDAMEAGLMPSLLDKAGELGLLGVSVPEQYGGFGKDFITGTLLTE
                     TLGAGHSFSVAMAAHTGIGTLPIMYFGNEKQKEKYLPLLASGQWKASYCLTEPGSGSD
                     ALAAKTKAILSSDGKHYILNGQKMWITNAGFADLFVVFAQVDGEKFTGFIVEKTYPGL
                     SLGEEEHKMGIKGSSTRQVFFSDCKVPIENVLGEIGKGHLIAFNILNIGRLKLCAAAL
                     GGAKHVSTHSVSYANTREQFKLPIAKFGAIRYKLAQQAIRIYACESALYRTAFMIDEL
                     EKQKLAEGIEYSQALLQAAEEYAVECAILKVDGSEALDYVADEGVQIYGGYGYSADFP
                     MDRSYRDSRINRIFEGTNEINRLLTVDMILKRAMKGKIDLMTPAMNIQKELMAIPDFS
                     EPDMSLFAQEKKFISNYKKALLAVAGSAVQKLMMTLAKEQEILMNIADMAIAIYISES
                     ILLRTEKLVNLKGEENCSVQLDMMRVYVNDAADRIALAGKEAINSFAEGDEQRMLLMG
                     LKRFTKVPSFNVKEARRRIAARLIEKNEYCF"
     gene            11410..12432
                     /locus_tag="PKU82_04720"
     CDS             11410..12432
                     /locus_tag="PKU82_04720"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOZ82211.1"
                     /translation="MIRKVFVPALILILLVSGFYYRHWLVYGFNQLKGQLNIIYNAQP
                     IGEVIENPLTPDSVKTKLILIRAIKKYAVDSLGLSETNNYETYYDQQGKPALWVLTAA
                     PEFKMVPYKWKFPFLGSLSYKGFFQKQKGVDELMLFRKNGFDADLSTVSAWSTLGWFG
                     DPVMSSMMRRNIGSLAELIIHEMTHTTLYIKGNVEFNENLATFIGEIGAEKFLTHKFK
                     DDSIVMTNYINHRNDNRIFSEYVIASVSKLDSLYSDKAFISLTYPKKLRLKYQKIASI
                     VLHIPKLPLKNDQRFEWNLQNKKLPDNTWFLSYSDYHALQPEINRVFRDSCNSSIQEL
                     IKYYKR"
     gene            complement(12445..13317)
                     /locus_tag="PKU82_04725"
     CDS             complement(12445..13317)
                     /locus_tag="PKU82_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82212.1"
                     /translation="MLITVVACKKDRVPQDDYQNMDSFYSDHEEEEQEYTIDTNGTCP
                     LVCKKGTKICVNGEMFELNGGGQVTYPFQLKVIELYSIKDMILRRIPSVASGNPLETS
                     AEIRVRAFKNGNEIFLKPGKKYFLETAALPSINSAMQSYYGYSSGNSANNWTNTISNF
                     IPGFVDTLSSISPTSNSYVTTPATTGWFSPALLHQATSTTTITCSVTGTNTQNLQVYV
                     SFKGFKGVMQIQNLSGTNFPVGENVTLIVFGKKQNNQYVLDQQSFTIAANQQIIFNLQ
                     DVSENDILNALAAL"
     gene            complement(13371..13877)
                     /locus_tag="PKU82_04730"
     CDS             complement(13371..13877)
                     /locus_tag="PKU82_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82213.1"
                     /translation="MFIAFNASAQRKVRKPIGYGASAIYNFQAEGIGADLRVRIPTPV
                     NNLYVVPEISYYPPFNRYHELYAGGALQYDILRIRSYNFYLLGAAYYNFWINADDYAP
                     EGKKKHNFAPEAGGGLVRNRGCWRPFIENRYDFKWKEDNLRIGIYYYPGQCRKNTRRE
                     KCPTVKGI"
     gene            complement(14002..14631)
                     /locus_tag="PKU82_04735"
     CDS             complement(14002..14631)
                     /locus_tag="PKU82_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOZ82214.1"
                     /translation="MKNVLVVGAHPDDLEWMAGGTVAKIISEGGKVHLITLTNGTWKD
                     ASGNYYRDKDIAISEAKKAASVLGYSIEHLDEPCLDVNFRDELVVQVLNRIDKINADT
                     IICPWIDDLHHDHEMTARIAVSASRRVPRVLMGMCNWYVFRTPFSPNFFVDISDTYSK
                     KMAALECYESEMKRIGSTWRKYHDNITSNYGLMVNVERAEGFISYKYKL"
     gene            complement(14665..15636)
                     /locus_tag="PKU82_04740"
     CDS             complement(14665..15636)
                     /locus_tag="PKU82_04740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ82215.1"
                     /translation="MAENYHYHSYTPSDLDEILALFSLSFNGLKLSSEYYKWRVLNTP
                     VNKNLIDLFKNDNGKIISHYAVCPVKSFYKGETSLSELSLITMTHPEFKSLGLFPKLA
                     AKLYERSFKEENIQLVYGFPNTNSHYSFVKKLAWNDVYMIPTMKKTFTTAVQGDKTYT
                     EIFNADQRFDQLWEQHTQKADRFYANVRNSTFINWRFFNRPDKKYRLFAFENNSNIQG
                     YFVIKEYANASTKEIDVVDFLFTHDFDTSVFFNSIAQLATELDAKTANMWIPYNTPLY
                     NLAEKSGYLPKEHITYLGYKPFNPGKNLDELRIPTNWYLTMCDSDVY"
     gene            complement(15648..16352)
                     /locus_tag="PKU82_04745"
     CDS             complement(15648..16352)
                     /locus_tag="PKU82_04745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82216.1"
                     /translation="MTENCSIVIPVFNEEEILEKRIHLLVEDLKKRFKDFEIVLTENG
                     SVDNTKSIIRTLSEKYDMVVAEIDDEKADYGQALINGINRARFEYITIFELDYLDMVF
                     LQKSNELLSSYDLVIGSKKLSGDLDRRPFKRKLFTWLYNTGLKVAFSYKLTETHGLKS
                     FKKSKLAPITNGCVTRHAVWPSEFCIRASRDKNLKVTEIGLSMPIEEIRTTRIKAIKR
                     LKKTIDDILLLRRSIN"
     gene            complement(16349..17635)
                     /locus_tag="PKU82_04750"
     CDS             complement(16349..17635)
                     /locus_tag="PKU82_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ82217.1"
                     /translation="MIHHDTVAVGAGLAGLSFAYHFDKNLPVFEALDTVGGLVRTIEY
                     KNCKFDLAPHLLHLRNPYVRELVFDKLGLEVENHRRKAHIYYDKTIIPYPFELNLLNL
                     SEQTKKDCIEGLDEVKSYNKEEEAEIRKGSYRDYALKCFGKGIANHYLLPYNRKIWDT
                     EPSEMTCEFMSHIITADKEQIRKNAFEDNTDSFGYNVEFYYPIHKGIQDLADIFARQL
                     TNIKFNTSAIFFNTRNKTIEFSDGSIVKYNKAVSTVPLQTIILNSDRDDLKALAKELV
                     FTSVYTVNLVIRGTLPDTHWMYFPDKDLSFYRISFPKNYFKKSTPADEQIIAVEVGSR
                     NHNLSIEEIERKVVEEIKQMPIFKIDELVFVHSIKIPVAYCIYEPKRPAIINKLMSEL
                     EEAGIYQSGRYGKWEYAGMQDAIMDGKVLSEKLKSL"
     assembly_gap    17791..17890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18028..>19883)
                     /locus_tag="PKU82_04755"
     CDS             complement(18028..>19883)
                     /locus_tag="PKU82_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOZ82218.1"
                     /translation="EEQSENKINTISDEITKEAEDLKNNVPVSFELEPDMDKAISDLM
                     NSGDEQKKVPDSNNLELELNIETPEVEKDEPTVKESDIPLAIENLNVTEDATEASEEN
                     DNSELPDQVTLGEYDPTLDLSSYQYPVLDLLENYQSKSKLTHEELKAELETNKNKIVE
                     TLGHYNIGIEKIKATVGPTVTLFEIVPQAGVRISKIKNLEDDIALSLSALGIRIIAPI
                     PGKGTIGIEVPNQSPEVVPMKAVLGSEKFINSDMDLPIGLGKTISNEVFVTDLAKMPH
                     LLMAGATGQGKSVGLNAILVSLLYKKHPSQIKFVLVDPKKVELTLFKTIERHFLAKLP
                     GEEEAIITDTRKVVNTLNSLCVEMDQRYELLKDAQVRNIREYNTKFVARKLNPNKGHR
                     FLPFIVLVIDEFADLIMTAGKEVETPIARLAQLARAIGIHLIIATQRPSVNIITGTIK
                     ANFPARLAFRVSSKIDSRTILDCGGADQLIGKGDMLLSLGADVIRLQCAYVETFEVEK
                     ITDFIGSQRGYPDAFLLPEFVGDNESNTGEVDLADRDPLFEDAARMVVQHQQGSASLL
                     QRRLKLGYNRAGRLIDQLEAAGIIGPFEGSKAREVKFKDPIELETLLSNLK"
BASE COUNT         6108 a         3236 c         3791 g         6648 t
ORIGIN      
        1 cctttagact tatgaatggt gatgatggta ataagagttg gaatatgcaa ggtgaactga
       61 ggagcattgc aaaagaaatt tttaaagatc accttaaagt taattttgaa actgacgttc
      121 ttgccgacac agaagggatt aatgaagtaa agagtttgta cgctgctatc tggaacaaat
      181 tgtcaggaat ggcaaagaaa tttttggaga tagcagattc agcaggagta gagcctattg
      241 atttttacta tggtagaggt ggatttcatt cttttatagt aaaaattgct gcaaaggttc
      301 atcctaaaga taatactata ggtcaacgta catggcagat ccttgatcgt gaagatgttt
      361 ttgcagataa tgcccaggat tttcttaaac aagatgtaag tctgaatggt gaattattgt
      421 ctttagtacg gcaaattgtt gattattata taaccaacat tgagcgattg ttatctgctg
      481 aagttgttac tgcaaatatt tatcaagccg taattcttaa atatctgttt aggcagttga
      541 aaatttatcg caaagatcat cagattttgc ttatgagtga tgtgggtaaa attttaaact
      601 ccattatcgg aaataatgat acacctttca tttatgaaaa ggccggaaac aaatacagaa
      661 attttttgat agatgagttt caggatacat cacaagtaca ttggaaaaat ttgttgccac
      721 tgctgataaa tagtttgtcg gagcagcatc aggttcttac tgttggcgat ggtaagcaat
      781 caatttaccg ctggcgggga ggagatatga atctgctttt aaatgaagtt gaaagtacac
      841 taaagaatca tgcaaatagc actgatatat tccggttgga ttctaatttc cgttcgtctg
      901 tttctgttgt tgagtttaat aatatttttt ttagtgcaat caatgaaatt gtatctgctg
      961 attcacaaca atcacaattg ctgaagaaag catatcaggc agatgtagtg catcaagttg
     1021 caaaacgcag tgaggaaaaa ggttttgtgt caattgaact ttatttggat aaagaagagt
     1081 atcctgagct aaattatccg gaatatttta gtgaagctga taaggtggtt gcatcacata
     1141 cgctaagtta cattcaaagt cagattgaaa agggatataa gcccggtgat attgcattgc
     1201 tcgtcagaac caatgtaaag ggaaacatgt tggcagactt cttggttttg aacggttata
     1261 ataatattaa ttctcgtgat tcattgttga tttgccatgc gcctcaaatc agatttttga
     1321 taaattgtct cagactcaat gctgataata aaaatcaggt agcagaaaag cacattttat
     1381 attttgtgtc taaacatctg cgaatggaaa ctaaactaat tgaaacaaat atcgaaaagt
     1441 atgttaagca tgttaaactg tattcgctga ctgaattttg tgcacaggta ataagtaagt
     1501 ttgggttgaa taggcaacct gatgcatatc ttcaatgctt tgaagacttg atttttgaaa
     1561 aatcaagtaa aggaattagt actactgcag agtttcttga ctggtgggat gttgaatcca
     1621 atcaaagtaa aatttcagtg caggttcctg ctgatgaaaa cgctattacc atcatcacca
     1681 ttcataagtc taaaggattg caatttccta ttgtaatcat tccttatact gattgggatt
     1741 taaatagtaa taaaggtttg atttggatca gaagtgatat ttcacctttc gataaattag
     1801 gtgcatttcc tgtcagacct tcaaagaaat tattgaaaac atattttggt caggattata
     1861 ttcttgaaaa ccgtgaaaaa gtattggaag aagtaaatgc actttatgtt gcatgtacca
     1921 gagcagaaga taaattgttt attttgattt caggtagtgg agaagaaggt gaggatggta
     1981 ttaataaagt taatacatta attaagtcgg cagtaatgaa tgctgaaact gttagttcaa
     2041 ctttgacaaa ggcaagtgat actgtttaca ctttgggagt tgattcagaa tatatgcaaa
     2101 agagaaagaa actatccgac acacctgata gttttactaa taaggagctg gtaaattatt
     2161 ttacaagtgt aaacaggata agcaccggat tcaaaattaa aaagtatgct gaacaaagta
     2221 aaagccgctt aatagggatt aaagttcatg agttgctggc tgattttcgc agtgaatctg
     2281 atttaaaagc aatagagaag catttaagta atcttcaatt accggatgac gaaaaaaatg
     2341 agttgctgaa tggcgtgcaa actacttgca attttctctc tgagaaaaaa tgggttagtc
     2401 cgcattttaa aatttataat gaacatgaaa tggccgatgc agaaggtaat attttaagac
     2461 ctgacagatt gatgattgaa ggggataatg ctgtggttat tgattataag acaggagcca
     2521 ttgaaacttc tcatgtgcaa caaatagcta attacaagga agttttgctg gatgcgggct
     2581 tcaaggatgt gaagactttt ttatattatc tggaagggaa agaattagtt gaggcttaaa
     2641 tgctttctat tttccatgcc aacggttgac aaattctgta accattggtt tagatttagt
     2701 ttgaggaaga ttaatcggtt tgttcaaccg ttctttaaag tgaacataat tgtcagaaat
     2761 catccaggat atggtgatct cattcaattc ttcatgtctt ataaaaacaa agtcgcaaat
     2821 acttttaaag ttagcaaaag agaatgctat gcaaggaaca aaaaatggct ctttactttt
     2881 tgaaagattt ctaatttcag gttcatagca attcaacagc ggaaattgct tcatgatgtt
     2941 ttttcctatc aaatcgctta cctgaaaaat tgtatcacag cttttaagaa tgttgccata
     3001 gttatcaaaa ataataatct ggcttttgct gtgaacgttt ttcatatcag taaattttga
     3061 ttttttgaaa gaatggctca ttcagtaaat cagccgctat tgtttgaaac atttcattaa
     3121 tcatgaaatt gttttttgct gatgtaaaaa aagttctgta gcaatcaaac tgtgtaaaga
     3181 aatcactgca gtcaagattg atattcgatt caattttatt gcaagttaag taaacaggcg
     3241 tgcctggaag aaatttctga taatgactaa tccatgcatc aagatctgaa aatgttgatg
     3301 ggttgtttac atcaactaca tgtattaatg catctgttcc aagataatat gacttgaacg
     3361 attttagatt cgaaacactt cctggagctg tccagataac aaaattgatc tttttcttat
     3421 ttatagccaa ctcttttctt gcaatgcata attgactatc ttctgaatag gaatgatgaa
     3481 atctgttatt caccaatcgg ttcacaatag aacttttacc gcattggcta tatcctgtta
     3541 tgagtatttt tttattctgc atgccgattg tttatagtgt ttatatgcag aaaagagaaa
     3601 aaggttgcag tattttgttt gaacttctga aaattattct tcttccatgt gatgggtttt
     3661 gccatctact gattttatgg ttttccaatt accgttttca tcttgttttt gtatttcata
     3721 gcgttcaaca ccttctttcc agcctttgta tggagtccat tctaatctgc tgttgttcat
     3781 gtcatctaat ttgccttgta gaagaatgtt tcctgataaa tctgtcagac ctgttctttg
     3841 ctgacatata ttttctactt caattttgta ttgataattt gtagcattga catccacatc
     3901 tctgtcaaga tagtattttg tgctgttgtt taccgaatct atcagaacaa aatttcccgg
     3961 gtctttgtct gatgatttat aaattaaata atagtaaacc aactcaggaa atgggttgcc
     4021 ttcatgccat tctgttaaaa tactgttgtt gtcaaccaca gttgaacgaa tgattgatgt
     4081 ggttatctga tttacaaaac ttgttggtac cgatgcatca ttattgcttt cagaagtgta
     4141 tatgctgctg tctaattgtg cttcaacttt ataactgtat tcctgaggac aatatgctgt
     4201 agaatcaata taaaaatctt ctgttcctgc aatttcatct atcaggtcat aattcaatcc
     4261 atcagtactt cggtaaatcg agtattgtga aattgtctta ccaatataag gtaaccatga
     4321 gagcatgttt gacattgtgt cgggctctgt tgttaataga attgatgcat gggatattga
     4381 actatccaat ggatatgaat tgttgcaatt attgattgtt tgaagtttgt aataatagcg
     4441 attctgattt gttgttacat tattgtcaat aaatgctaat tgcagattca atgtgctgtt
     4501 gtgtgtattt gtatctgtgt aaatcagatt gtaattttta tcgtcagttg atctgtataa
     4561 ttcaaatctg tttagattgg tagctgatga tggttgccat gatattctga ttgcattgct
     4621 atcgagtacg gtgaggtaat ttatttctga aggtgttatt ggttgattgt tttgtgcagc
     4681 tatgaagttg agtttagtaa tactatcact gcaaaggcct tcattagtga cttttaatgt
     4741 cactgtataa tatcctgcat tttgataatt aaactgcgga ttgaatgctg atgaagtgag
     4801 tccattgccg ccatcccata taaatgcagg gttctgtagt gatgagctga tgttgaccat
     4861 gtttgaagaa agcggtgcac aaccaatcaa tggcgaacct ccaaaatcgg caatggggaa
     4921 aggtataacg gtaacggcat tgatgctgtt agctgtatcc gaacaatgac cattagaagc
     4981 aactaatgta atgctaaatt gccctggaat ctgatattgg tgacttgtat ttgactgatt
     5041 cgatactgta ccatctccaa attgccattt gtaactactt gatgattgag attggttgag
     5101 taaagaaatt gaagttccaa cacaaattgt agagtcgctg attgcaaatg ctgcaacagg
     5161 tttgttgtaa actttaattg tatcaggatg tgtataaaca gattgacatc cattggcatc
     5221 ctttacaatc aatgttatta agtacatacc ggcagtattg aacgtgtgaa tgggtgaagt
     5281 tgccacagaa gtatctccat ctccaaaact ccaaaagtga ctaagtgcat tttgagagga
     5341 gtcgttagtg ctgatagtta aaggttgaca accatgattg tttgaaagag aaaatgaagc
     5401 taagggtcct ggtatgtgta aagcattgga caatgttttt gtattactgc aacccgatgc
     5461 atctgttact gtaagtgtaa cttggtagtt tccgcctgtt gtgtagatat gatttggatt
     5521 caagagtgtt gatgtactgc catctccaaa atcccaaaga tagtttgcag cacccgaaga
     5581 tgtattttga aattgaacaa tcaccggaac acaacctgaa gattttgtat aactaaaatc
     5641 agcctgtggc tctgtgatgg agattatttt tccaccaaca gtatctttac atccaaaaga
     5701 atctgtaatt attaaagatg gtataaaatt gccggtacta ctgtaattaa tacttggatt
     5761 tggagttatt gatgttgacc cgttaccaaa tttccataac cattgatgtg catttttact
     5821 tgaatctgta aattgtatgg tttttggatt acatcctatt gtatctgact gcgtaaattc
     5881 cgctaccgga agttggatgt tatcaagctc ataatttttt tcgcatccat taatatcttt
     5941 agcaaaaagc tttattttat ctgttggtaa agaggtaaag gtatgttgtg tagcctgagt
     6001 aaaagtggtg tcaccatcgc cccaagacca tttccatgct acagcattta ggcttgaatc
     6061 agttacatta attgtgatag gtaagcaacc ttgacttctt gaataactga atttaacatc
     6121 aaaatctggt atggtaacag cctgagctaa tgttttagtg cttgcacatc cattgttgta
     6181 tgcagttagc ttaatatcat aagtgtaggg tggatttggt ccatagtagt aatatttatt
     6241 tacatcggtt aatgatgaca tagttccatc tcccaaatcc catgtataag aagtgctgcc
     6301 tgtagaggta tttgttaact gtacattcac catggtgcat gatgaggaaa gcatattgat
     6361 gttgaagtca gccactgatt ttacaatggt tatttgttgt gaagcattta ctgtttgcgt
     6421 acaaccgtta atatccgtta taacaacctg aacagaataa gatcctgaat cagagtatgt
     6481 gtgagctata ggatttcctg ttgccgaagt gccatcacca aagtcccaag tatagtttac
     6541 atagtttgtg ttttgaactg aaaaggtaat agtgtctgat gcacatgctc tgtttttatt
     6601 ggaaagaata gcgttggcat cagcattggc atataaactc ttggaatgaa ttacagtaca
     6661 tccactagta tcttgtgcct gtaaagaaat aatgtaattg cttgtattgg catagctatg
     6721 tattggtgaa acctgtgagc ttgtttgatt atcaccaaaa tcccaattat agctttgcag
     6781 attcagattt gatggtaaag cctgaatgtt tgagccatta catgtattaa ctgtattaac
     6841 aataaatgtt accggtgttc ctgccacttt tacagcctgt gaatgataaa tagtatcaac
     6901 acttgcatta gataaatttg caatgagcat tacatcgtaa atgccgggta cgttgtatgt
     6961 gtgtaccgga ttttgttgag acgaagtatt gccatcacca aaatcccaat acgagccaac
     7021 aacatttaaa aatgggttat tgaaacttat ggtcaatggt gcgcaaccat cataatgcat
     7081 ggtgaattgt gtcgtatcat ggttttcatc ataaaatgtt cctccacttc caaagttagt
     7141 aggagggaaa ctgtaggtat aactacaact gtcgttggca atcgtcagac ttattgtgaa
     7201 agggcctgtt gattgatagg tgtgcatagg attttcaagt gttgaagaat ctccatcgcc
     7261 aaaagtccat agccatgatg ttgcacctac tgagttggcc gaaaactgaa catcaagagg
     7321 aggaatggta tctaatgtat gggcagaagg tgcagcacct aaggttaaaa agttcattgc
     7381 atattgttca acctttgttg atgtacatcc atttgcagaa gtgatggtta aagaaacatt
     7441 gtaactaccg gtttggttgt aggtgtgaac aggattttgt tgtgttgatg tttgtccgtc
     7501 accaaaattc cataaccatg aaacagcatt taaactgctg tctgtaaatg ttccgataaa
     7561 tggcggacaa agtgtttggt tatatgtaaa attagctata ccaccaccaa caatatacgt
     7621 ggcaaaattg ctgatggttt gagaacatgt tgagccattg ctttgcgttt gcagacttac
     7681 tgtatatgtt cccggtgtag tatatgtatg agaaacattt tgtgctgtgg cagtactgcc
     7741 atcaccataa tcccaaagcc atgagtttgc acctgatgag tcgcttaatg aaacagtaag
     7801 tggtgcgcat cctgatacgg gttgcggatt gctgaagttg tttacacttg gtccggggtt
     7861 gatgtaacct tgtttacgta atgtgtcttt acaaccattg ccatcagaag caattagagt
     7921 cacattataa ctgccttgat tttgatagag ataggttgga ctgtttactg ttgaagtatc
     7981 tcctgtgcca aactgccaaa gccatgaagt agaacctgta cttgtatttg caaagtttac
     8041 agttaatgga ttacatccag ttactttatc tgcaagaaaa tcggcttcag gtccgttgac
     8101 cgtaattcca tagggaagat ttgatgtagc tgtacatccg tttgcagcag taacggttag
     8161 cgcaggatag aatgtccctg attgtgtgta attatgaatg ccatttaatt gtgttgagct
     8221 attgccatca ccaaaactcc attgatagct acatcctgtg catgatgtag tttgaaatga
     8281 aatactcaat ggtttacatc ctgaagtatt cgatgtagtg aatgttgaga ctggagactg
     8341 taatacattt attgttaatg gtgcagactg gcttgaacag ccgttaggat agtttgcggt
     8401 tacataagga ttgtagtttc ctgcattgtt atataatact ccaactgttg atagtgtaga
     8461 tgtctggcca ttgtctaagt tccatagaag ggatgatgtt cctgtagtat tagttgataa
     8521 aacaacatta aacggattac aacccgatgt gatattggat gtaattatgg gtataggatt
     8581 gttaagtact gttatcggtg aagatagatt tacggtagat gagcaaccgg aggctgatgc
     8641 agtaactgca acctgatagt ttccggattg tgaatattga tgttgaacaa ttgcgcctgt
     8701 tgcagttcct ccatcgccaa aattccattg ataagatgat gtggtatttg caccagcatt
     8761 aaaatgaaat attttattga tattacatgt gctgaatgtg tcaaccgttg ctgttacaat
     8821 tggaataggc ttaataataa tgatgttatt tttcgtcagt gtttgtgtgc atgttgagtt
     8881 gagcctgaac aagactaaag taacattata agttccggct gttgcataaa tatgtgtagg
     8941 attcgtttga ttgcttgtaa caccatcacc aaaatcccat tgacaactgt caaaaattgc
     9001 actttgattg gtaaagttta taatttcacc ctgacatgct gaagatttat tgagtgtaaa
     9061 ttgtacaaca ggtttgtttt ctatatgtat aggttgcgaa gaaattacag aatcactgcc
     9121 tgaagaattt gttgcgatga gttttacata ataatttcct gcagtagtaa aagtaattac
     9181 aggactattc aaaacagaaa ctgcaccatt gccaaaatcc caatgatagc ttgttgcatt
     9241 ggtactcgta ttgttaaatg aaacagttag aggtgcacaa cctgatgact gactgatgcc
     9301 aaatgatgca accggtagtt gtgcaatact ttgatgtagc gaaaaaataa tcactaaggc
     9361 agtaagccac attcctgaca ttttatttaa agcactattt cctcccacta aaaagttgat
     9421 tttagcaagt tttacggtaa agtgcattaa aagttataat tcttacactt taatattatc
     9481 tgtttgtttt tcagtagatt taatttccgg tattttagaa atattgaaac cttaaggctg
     9541 gtatttatgt ttaatatttt tatttttatt gcgttaaaaa aaccactttt attatgtcag
     9601 caattcagga aaagaaccaa tcggttaagg ggggagaatt tattattaaa gaatcaagtg
     9661 cagagaacac ttttataccc gaagagttta gcgaagagca tttgatgatt gagcaaacag
     9721 caaatgattt tttaaaacag gaagtacata cagtactcga tcgtattgat gcaatggaag
     9781 caggacttat gccatcattg ttagataagg caggagaact tggacttctt ggtgtttcgg
     9841 ttccggaaca atatggtgga tttggaaaag attttattac aggcacatta ctgacagaaa
     9901 ctttaggagc aggtcattca ttttctgttg caatggcagc acatacaggt atcggaacat
     9961 tgccaataat gtatttcgga aatgaaaagc agaaagaaaa atatttaccg ctattagcat
    10021 caggtcagtg gaaagcatct tactgtttaa ctgaacccgg ttccggttct gatgcgcttg
    10081 cagcgaaaac taaagctatt ctttcttctg atggaaagca ttatatcctt aacggacaaa
    10141 aaatgtggat taccaatgca ggctttgcag atttgtttgt agtatttgca caggttgatg
    10201 gcgaaaagtt caccggtttt attgtagaga aaacttatcc gggactatca cttggcgaag
    10261 aagaacataa gatgggaata aaaggctcaa gtacaagaca ggtttttttc agtgattgta
    10321 aagtgcctat agaaaatgtt ttgggcgaaa ttggcaaagg acatcttatt gctttcaata
    10381 ttttaaatat tggacgtttg aaattgtgtg cagcagcatt aggtggcgca aaacatgtaa
    10441 gtacacactc tgtttcctat gcaaacacac gtgagcagtt taaattgcct attgcaaaat
    10501 ttggtgccat cagatataaa cttgcgcaac aggcaatcag aatttatgct tgtgaaagtg
    10561 ctttgtatcg tacagcattt atgatagacg agctcgaaaa acaaaaactt gcagaaggca
    10621 ttgaatattc tcaggcatta cttcaggctg ccgaagaata tgctgttgaa tgtgccattc
    10681 ttaaagttga tggttctgaa gcacttgatt atgttgcaga tgaaggtgtg cagatttatg
    10741 gtggttatgg ctatagtgca gattttccga tggacagatc atatagagat tcacgcatca
    10801 acagaatttt tgaaggaaca aatgaaatta acagactcct tactgttgat atgattctaa
    10861 aacgtgccat gaaaggtaag attgacttaa tgacaccggc aatgaatatt cagaaagaac
    10921 ttatggcaat tcctgatttt agtgagcctg atatgtcgct attcgcacaa gagaaaaagt
    10981 ttatttctaa ctataaaaaa gcgttacttg cagttgcagg ttctgctgta caaaaattga
    11041 tgatgacact tgctaaagag caggagattc tgatgaacat tgcagatatg gcaattgcaa
    11101 tttatatttc agagtcaatt ttattgagga cagagaaact tgttaattta aaaggtgaag
    11161 aaaattgcag tgttcagctt gatatgatgc gggtttatgt aaatgatgca gcagatagaa
    11221 ttgcattggc aggaaaagaa gcaattaatt cttttgcaga aggtgacgaa cagagaatgt
    11281 tgctcatggg attaaaacgc tttaccaagg ttccgtcatt caatgttaaa gaagcaagaa
    11341 gaagaattgc agcacgctta attgaaaaga acgaatactg cttctgattt gtagagcagt
    11401 tctaagctga tgatcaggaa ggtttttgtt ccggcactca ttttaatttt attagtttca
    11461 ggattttatt accgccattg gttggtttat gggtttaatc aactaaaggg tcagctaaat
    11521 attatttaca acgcacagcc tattggcgaa gtaatagaga atccattaac tcccgattca
    11581 gttaaaacaa aactgatatt aatccgtgca ataaaaaaat atgctgttga ttctttagga
    11641 ttatcagaaa ctaacaacta cgaaacttat tatgatcaac aagggaaacc tgcattgtgg
    11701 gtgcttaccg ctgctcctga atttaaaatg gtaccatata aatggaagtt cccatttctt
    11761 gggtctttat catataaagg attttttcag aaacaaaaag gagttgatga attgatgttg
    11821 ttcaggaaaa acggctttga tgcagatttg tcaacagtat cagcatggag tacccttggc
    11881 tggtttggtg atcctgtaat gtcttcaatg atgcgaagga atattggctc gcttgcagaa
    11941 ttgattatcc atgagatgac tcatacaacg ctttacatta aaggaaatgt tgaattcaat
    12001 gaaaaccttg cgacttttat tggtgaaatt ggtgctgaaa aatttctgac tcataaattt
    12061 aaagatgaca gcattgttat gacaaattac atcaatcatc gcaatgacaa cagaatattt
    12121 tcagaatatg taattgcttc agtatctaaa ttggactcat tgtatagtga taaagctttt
    12181 atatcattaa cctatccgaa gaaactacgt ctgaaatatc aaaaaatagc atcaattgtt
    12241 ttgcatatac cgaaattacc attgaaaaat gatcaaaggt ttgagtggaa tctgcaaaat
    12301 aaaaaactgc ccgacaacac ctggtttcta tcttattctg actaccatgc tttgcagcct
    12361 gaaattaatc gggtgtttag agattcctgc aatagttcaa ttcaggaatt gataaaatac
    12421 tataagagat aaggcgtaat ttacttacaa tgctgcaaga gcattcagaa tgtcattttc
    12481 cgaaacatcc tgaagattaa aaataatttg ctgattggca gcaattgtaa aactctgctg
    12541 atctaaaacg tattgattgt tttgtttttt accgaatact atcaatgtta cgttttcgcc
    12601 aacaggaaag tttgtgccac tcaggttttg aatttgcata acacctttaa aacctttgaa
    12661 tgaaacatat acctgtaagt tttgcgtatt tgttccggtg acactacatg taatagttgt
    12721 tgttgatgtt gcctgatgta ataatgcagg agaaaaccaa cctgttgtgg caggtgttgt
    12781 tacataactg ttagaagttg gagaaatact cgatagtgta tcaacaaaac caggaataaa
    12841 attagaaata gtattggtcc agttattagc actattaccg ctgctataac cgtaatagga
    12901 ttgcatggct gaatttatag atggcaatgc agctgtttca agaaaatatt ttttgccggg
    12961 tttgagaaaa atttcatttc cgtttttaaa tgctctcact cttatttctg cacttgtttc
    13021 aagtggattt cctgaagcca cagaaggaat tcttcttaaa atcatatctt taatagaata
    13081 caactctatt acttttagct gaaaaggata agtaacctgg cctccaccat ttaactcaaa
    13141 catttctccg ttgacacaaa ttttagttcc tttcttacaa actaatggac aagttccatt
    13201 ggtgtcaatg gtatattcct gttcttcttc ttcatggtcg ctgtagaatg aatccatgtt
    13261 ttgataatca tcctgtggca cacggtcttt tttgcatgca actactgtaa taagcattat
    13321 tgcagtaaga taaatgaata gcttgttcga tttcatatta caaagtttga ttaaattcct
    13381 ttaactgttg gacatttttc tcttcttgta ttttttctac actgtccggg ataataataa
    13441 atacctattc gcaaattatc ttctttccat ttaaagtcat agcggttttc aataaatgga
    13501 cgccaacatc ctctgtttcg caccaagcca ccaccggctt cgggagcaaa attgtgtttc
    13561 ttttttcctt caggagcata gtcatctgca tttatccaga aattgtaata ggcagcacca
    13621 agtaaataaa aattataact tctgattctt aatatatcat attgcaaagc cccaccggca
    13681 tacaattcat gataacgatt aaaaggaggg taatatgaaa tttcaggaac tacataaagg
    13741 ttatttaccg gtgttggaat tctgacacgc aaatctgcac caataccttc ggcctgaaaa
    13801 ttataaattg cactggcacc gtagcctata ggtttacgta ctttacgttg tgctgaagca
    13861 ttgaacgcaa tgaacattgc aaacaaaata atggatatat gcctgatttt aaacattgaa
    13921 atattttggg attaaagatg ttaactttat ctattcccaa aacggcatta ctggaaaatt
    13981 attatttgat tataaacaga actacaattt gtatttgtag ctgataaaac cttctgcgcg
    14041 ctctacattc accatcagac cataatttga agtgatgttg tcgtgatatt ttctccaagt
    14101 gctcccaatg cgtttcattt ctgattcata gcattctaat gctgccatct ttttagaata
    14161 tgtgtcgctg atgtcaacaa aaaagttagg actaaatggt gtacgaaaca cgtaccagtt
    14221 gcacatgccc atgagtacac gtggcacacg cctgcttgca ctcactgcaa tacgtgcagt
    14281 catttcatga tcgtgatgta aatcgtcaat ccatggacat attatcgtat ctgcatttat
    14341 tttgtcaatg cggtttaata cctgaactac taattcatct ctgaaattta catcaagaca
    14401 aggttcatct aaatgttcta ttgagtagcc taaaacagaa gcagcttttt tagcttcact
    14461 gatagcaata tctttatcac ggtagtaatt tccggaagca tctttccatg taccattggt
    14521 taaagttatt aaatggactt tacctccctc tgaaatgatt ttggcaacag taccaccggc
    14581 catccattcc aaatcatcag gatgtgcacc tactactaaa acatttttca tgttgtcatt
    14641 gattttaaat tcaatagtaa cctatcagta aacatcagaa tcacacattg tcaaatacca
    14701 gtttgttggt attcttaact catctaaatt ttttcctggg ttgaatggtt tatatcctaa
    14761 gtaagttata tgttctttcg gcaaatagcc tgatttttca gcaagattat acaatggagt
    14821 attgtaggga atccacatgt ttgctgtttt agcatctaat tctgttgcta attgtgctat
    14881 ggaattaaag aatactgatg tgtcaaaatc atgtgtaaaa agaaaatcaa caacatcaat
    14941 ttcttttgtt gatgcatttg catattcctt aattacaaaa taaccttgta tattactgtt
    15001 gttttcaaat gcaaataagc gatatttttt atcagggcga ttaaaaaaac gccagtttat
    15061 aaatgtgctg tttctgacgt tcgcataaaa acgatctgcc ttctgtgtgt gttgctccca
    15121 tagctgatca aatcgttggt cggcattaaa aatctctgta taagttttat caccttgaac
    15181 agcagttgta aatgtttttt tcattgttgg aatcatatat acatcattcc aagctaattt
    15241 ttttacaaag ctgtaatgcg agttggtgtt tggaaaccca taaaccaatt gaatattctc
    15301 ttccttgaag gagcgctcgt aaagttttgc tgcaagtttt ggaaacagtc ctaatgattt
    15361 aaattcagga tgtgtcattg tgatgagcga aagttcactt agtgaagttt cgcctttgta
    15421 aaaagatttt accggacaca ctgcataatg acttattatt ttaccattgt catttttaaa
    15481 taaatcaatc aggtttttat ttacaggagt atttagaaca cgccatttat aatattctga
    15541 tgagagtttt agtccgttaa atgaaagaga aaataaggca agtatttcat caagatctga
    15601 tggagtgtaa ctgtgataat gataattttc tgccatagcg tattttatta gttaatagat
    15661 ctgcgtaaca gcaaaatatc gtcaatagtt ttctttaatc tttttattgc tttaatacgg
    15721 gtagttctga tttcttcaat cggcattgat aagccaatct ctgttacttt taaattttta
    15781 tcacgtgaag ctcttatgca aaattcactt ggccaaacag catgtcgtgt tacacagcca
    15841 ttagtgattg gagctaattt acttttctta aaacttttca gtccatgagt ttctgttaac
    15901 ttgtagctaa atgcaacttt aagccctgta ttgtagagcc aggtaaaaag ttttctttta
    15961 aatggtcttc tgtcaagatc gccagaaagt ttttttgagc caataacaag atcgtatgag
    16021 ctaagcaact catttgattt ttgtaaaaag accatatcta aatagtcaag ctcaaatatg
    16081 gtgatgtact caaaacgtgc ccgattaatg ccgttaatca aagcctgacc atagtctgct
    16141 ttttcgtcat caatttctgc aacaaccata tcatactttt cagatagtgt tctgatgata
    16201 gatttggtgt tgtcaacgct tccgttttct gttagtacaa tttcaaaatc tttaaaacgc
    16261 ttttttaaat cctcaacgag caggtgtatt cttttttcaa gaatttcttc ttcgttaaat
    16321 accggtatta ctattgaaca attttctgtc ataatgattt taatttttcc gacaatactt
    16381 tgccgtccat aattgcatcc tgcatgcctg catattccca ttttccatag cgacctgact
    16441 gataaattcc tgcttcttcc agttcactca tcagtttgtt gattatggca gggcgttttg
    16501 gttcgtatat acaataggcc acaggtattt taatcgagtg aacaaatacc aattcgtcaa
    16561 ttttgaatat tggcatttgt ttaatctctt caacaacttt gcgttctatt tcttcaatac
    16621 tcaggttatg attgcgactg ccaacttcaa cagcaataat ttgttcgtca gcaggtgttg
    16681 attttttgaa atagttttta gggaagctta ttctgtagaa cgaaagatct ttatcgggga
    16741 aatacatcca atgtgtgtca ggcagtgttc ctctgataac taaatttact gtataaacag
    16801 aggtgaaaac caattctttg gctaatgctt tcaagtcatc tctgtcactg tttaatatga
    16861 tagtttgtag gggaacagta gaaacagctt tgttatattt tacaatactt ccatcagaaa
    16921 attcaattgt tttattccgt gtattaaaaa atattgcaga agtattgaat ttaatattag
    16981 tgagttgtct agcaaaaatg tctgctaaat cctgaatacc tttatgtata ggataataga
    17041 attctacatt atagccaaaa ctgtctgtgt tgtcttcgaa tgcatttttt ctgatttgct
    17101 ctttgtctgc agtaataata tgcgacataa actcacatgt catttcagaa ggttcggtgt
    17161 cccaaatttt tctgttgtaa ggtagaaggt aatgattggc aatacctttt ccgaagcatt
    17221 tcaatgcata atctctgtaa gagccttttc tgatttctgc ttcctcttcc ttattatatg
    17281 attttacttc gtcaagtcct tcaatacaat cttttttggt ttgctcactc agatttaata
    17341 gattgagctc aaaagggtag gggatgatgg ttttatcgta atagatgtga gcttttctac
    17401 gatgattttc aacttcaaga cccaatttgt caaatactaa ctctcttaca taagggttgc
    17461 gtaaatgtaa taagtgtggg gctaaatcaa atttgcagtt tttatattca attgtccgca
    17521 ccaaaccacc tacagtgtca agggcttcaa aaaccggtaa attcttatca aaatgatagg
    17581 caaaacttaa tcctgccagg ccggcaccaa ctgcaactgt gtcgtgatga atcatgtaga
    17641 aaaataagga gatttaaaag gctgcaaaag taaggatttt tatccttcgt tcaaagccca
    17701 ataattacct taaaagagga tactttatct gaggtcaact acctctacac ccggatattt
    17761 ttttgcatca aaattaaaat cgctgtcttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn acaaggaata tcatcaattt ttttgtcatt tcttaaattt taaatgtgat
    17941 taataaaaac aatgccacaa ggcaaaacga ggtgaaatgc acatgaacaa ttgcaaatat
    18001 aataatgtag gaggattaaa aacagaatta tttcaggttt gaaagtaatg tttcaagttc
    18061 tataggatct ttaaacttta cttctctggc tttgctacct tcaaaaggac caataattcc
    18121 agcagcttca agttgatcaa tcaacctgcc ggcacggtta taaccaagtt tgagtcttct
    18181 ttgcaataaa gatgcagaac cttgctgatg ttgcacaacc atacgtgcag catcttcaaa
    18241 caaaggatca cggtcagcta aatcaacttc gcctgtgttg ctttcgttgt caccaacaaa
    18301 ttctggtaaa agaaatgcat cggggtagcc tctttgtgag ccaataaaat ctgtaatttt
    18361 ttcaacttca aaagtttcaa cataggcaca ctgcaaacga atcacatcag cgccaagcga
    18421 caataacatg tctcccttac caataagttg atcggcacca ccacaatcta aaattgtgcg
    18481 actatcaatt tttgatgaaa ccctgaatgc caggcgtgca gggaagttgg cttttattgt
    18541 tccggtgatg atatttactg atggacgctg agttgctatg atgaggtgaa taccaatggc
    18601 tcgtgctaac tgcgctaatc gggcaatggg tgtttcaact tccttacctg ctgtcattat
    18661 caggtcggca aactcatcaa taaccaaaac aatgaatggc agaaagcggt ggcctttatt
    18721 ggggttaagt tttcttgcaa caaattttgt attgtactct ctgatgttac gaacctgagc
    18781 atctttaagt aactcgtagc gctgatccat ttcaacacat aaagagttaa gggtgttcac
    18841 tactttgcgt gtatcggtaa tgattgcttc ttcttcaccg ggaagttttg ctaaaaaatg
    18901 tcgttcaata gttttgaata aagtaagttc aactttttta ggatcaacca aaacaaattt
    18961 aatttgtgat ggatgctttt tgtaaagcaa ggaaaccaga attgcattta agccaacaga
    19021 tttaccctga cctgtggcac cagccatgag caagtgtggc atttttgcta aatcggtaac
    19081 aaatacttcg ttcgatatgg tttttccaag tccgatgggt aggtccatat cggaattgat
    19141 aaatttctca gagcctaata ctgctttcat cggtactact tcaggcgatt gattaggtac
    19201 ttcaatgcca atagttcctt tgcccggaat aggggcaatg atacgaatac ccaaagctga
    19261 aagacttaaa gcaatatcat cttcaagatt cttgattttt gaaatacgta caccggcttg
    19321 tggtacaatt tcaaatagag ttactgtagg tcctactgtt gctttgattt tttcaatacc
    19381 aatattatag tgaccaagtg tttcaacaat tttgttttta ttggtttcca actcagcttt
    19441 taactcttcg tgtgtaagtt tagatttact ctgataattt tccagtaaat ctaatacagg
    19501 atattgatat gaagataaat caagtgttgg gtcatactct ccaagagtaa cctgatcggg
    19561 aagttcgctg ttgtcgtttt cttctgatgc ctcagttgca tcttcagtga cattcagatt
    19621 ttcaattgca agtggaatat cactttcttt aactgtgggc tcatcttttt caacctctgg
    19681 tgtttcaatg tttaattcta attcaagatt attggaatca ggtacttttt tttgttcatc
    19741 accagaattc attaaatccg aaatggcttt gtccatatca ggttctaatt caaaactaac
    19801 gggaacattg ttttttaaat cttctgcttc tttagtaatt tcatctgaaa tggtgtttat
    19861 tttattttct gattgttctt cag
//
LOCUS       DAOOOL010000037        19796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_210_length_19796_cov_106.404235, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000037 DAOOOL010000000
VERSION     DAOOOL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19796
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_19796_cov_106.404235"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /gene="secA"
                     /locus_tag="PKU82_04760"
     CDS             complement(<1..1147)
                     /gene="secA"
                     /locus_tag="PKU82_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194069.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOZ82219.1"
                     /translation="MIDFINKTISKIFGSKAESDYKALSPVIEAVNAVAENMSSLSND
                     ELRNKTDEFRTRIQDYLSEIDTELTQLNEEAEADPEMDANQKEEIYNRIDALKKERNK
                     KTEEVLNEIQPEAFALVREVAKRFTENAELAVEANDNDKNLVTKRDGIRIEDGKAIYS
                     NTWTAAGSKVLWNMIHYDVQLIGGSVLHQGKIAEMATGEGKTLVATLPIYLNALAGAG
                     VHVVTVNDYLAKRDAEWNGPLFEFLGLSVDCIDKHQPNTEERRKAYLADITYGTNNEF
                     GFDYLRDNMTRHAHEMVQRPHHFAIVDEVDSVLVDDARTPLIISGPTPKGDTQEYFAL
                     KPKVEILFNAQRNYINTVIADAKKKLGENKPDEAGMPLIRAYRGLPKN"
     gene            complement(1348..1857)
                     /locus_tag="PKU82_04765"
     CDS             complement(1348..1857)
                     /locus_tag="PKU82_04765"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HOZ82220.1"
                     /translation="MKQANLFFVPITILLLSAIFSCNTSKKVSENNSADSLLISMSRF
                     PCFGRCPYYDASLYKSGYAVINRKGFMDSLGIYATQIDKQELNNFVEDAKKAGLIAMP
                     DSFYNPGIADYPATIVTVNLNGKRKRIFDGVPESPSSLKDFEEKVHLFFTAPNRQWKL
                     IQKPAQQQD"
     gene            1955..3256
                     /locus_tag="PKU82_04770"
     CDS             1955..3256
                     /locus_tag="PKU82_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOZ82221.1"
                     /translation="MKSIKIMGVLLLSIATVALLTSMKKGSGRYKVKTKGEKGCVFRS
                     VFEAEGIDSNFIYSTPVKVKESMTSALEWISKAQLKDGGWGAGSHARQSVFDPHAVSS
                     DPATTAMVAMALLRTGSTPTTGKYATNLSLATEYLLKQIESADAQTLNITNQTATQPQ
                     VKLGQNIDVVLATQYLTNLMDYISNNQTLKTRVSKSLDKCVVKIQHAQSENGSIKGAG
                     WAGVLQSSFATNALESAKAAGANVDEEKLERYKNYQKGNYDAKTNSVATGDGAGVMLY
                     SISGTARSTAREASIAKDKISRAKKEGKLAPTSTVTSESLQAAGMSEADAYKYAAAYE
                     INQAASKQAQRNDVMNGFGSNGGEEFLSYLQTGEGLIISKDKEWHKWYDNVSGRLLSI
                     QNNDGSWNGHHCITSPVFCTATSLLILSVNNDIEKLVSLNK"
     gene            complement(3299..4366)
                     /locus_tag="PKU82_04775"
     CDS             complement(3299..4366)
                     /locus_tag="PKU82_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006071841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOZ82222.1"
                     /translation="MTEKRELSAFEAKNEAQKLAFAPVVFQVVVAMQRLGILPLICKN
                     RKGITVKEISEKTNVSIYGVKVLLEMAANADIVKYLDDSTVTVTMLGYFLNSDEMTRV
                     NINFTQDVCYDGFKFLTESIQNGKPEGLKTLGNWPTIYPALSSLPEHIKKSWFEFDHF
                     YSDEAFRDALNIVFKENPSTIFDIGGNTGKWAFASCEFNPDVHVKILDLPQQIAVAKA
                     NAEKRNLTERISYFEINLLDKSQKIPKGADVVWMSQFLDCFGEDEIVSILENVRQAAS
                     PHTTVFILEPFIDNQKFEAASYCLTATSLYFTALANGNSKMYSVKAMTGLVEKSGLEV
                     VETFPLIGESFHTILKCKVKV"
     gene            complement(4392..5117)
                     /locus_tag="PKU82_04780"
     CDS             complement(4392..5117)
                     /locus_tag="PKU82_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOZ82223.1"
                     /translation="MNKIFRFHLSRHILWFCLLSFFLSSCRGIFLSKDKAYSKAIATK
                     PYDAIIVPGFPYFEGQPWPPVVQMRMIWAKFLYQNGYTKNIIFSGNAVYSPFVEGKTM
                     KAFAIAMGIPEEHIYVEDKAEHSVENVYYSYRLAKKLGFKKIALATDPVQTRSMVSFI
                     HRFSLPISYLPIMYKTLRLLPHSEPDIDGKLQRVENFISLPEKETFKQRFAGTLGKNI
                     KWHAEDLKKKRLRKKYSHKIVQE"
     gene            complement(5122..5751)
                     /locus_tag="PKU82_04785"
     CDS             complement(5122..5751)
                     /locus_tag="PKU82_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82224.1"
                     /translation="MKKTYLTLIFSFIMLNAFSQEKMNELFKNNVKLIFMGYDFSQAR
                     FIGTDGFTDPAAIKDKFLSAWNNTFVAEYEKFSLQSAFKLTNDKYETNVDVVQDINSK
                     INVHERVMNGSYSIGEDDVKKAVSHYKSKEKDAIGLVYVVESFNKTLEKAVIWVTFVD
                     LSNNKMLYTEKVEEKAGGFGLKNYWIAPATKLKKEIESKKYKIWKNKFK"
     gene            complement(5777..7063)
                     /locus_tag="PKU82_04790"
     CDS             complement(5777..7063)
                     /locus_tag="PKU82_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ82225.1"
                     /translation="MKQLGEIEFAGLDAIRKMQQQKLSELLQYVNAKSAYYKEVFRQH
                     NIKFDSITLDNLNELPFTTKDDLATQNDSFLCVDKSFIADYVTTSGTLSDPVTFYLTS
                     ADLDRLAYNEAISLQCADGSSADIFQMMTTIDKLFMAGLAYFLGIRKLGAASIRVGPG
                     TPQAQWDYIQRFKPTTLIAIPSFIPQLLQYAAERKINFQSTSVKKIVCLGEPVRNADF
                     SLNELGKRITSLWDVKLYSTYASTEMSSAFTECGYGNGGHAHPELLILEVVDDNGLQV
                     RNGELGEVVVTTLGVEGMPLIRFKTGDLCHAHYDLCKCGRYTTRLGPVMGRKQQMIKF
                     KGTTLFPQIIFNVLNMMPQIELFQVEVKRNEFNNDSITILLSKELHSDAFEHEISSLF
                     KSKIRVTPAFRYIDTEELQQKVFKTDKRKSEKIIYI"
     gene            complement(7063..8742)
                     /locus_tag="PKU82_04795"
     CDS             complement(7063..8742)
                     /locus_tag="PKU82_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOZ82226.1"
                     /translation="MPSFKGSLRFIFKFLLWTAGILIILFLLFLWRIQIPDPSISSKL
                     KPDDLHRIQIGVDSYRVGNNWLRKNQQGIWEMYIEGNDYERGVVYGVLAKELIEKQEE
                     YFVDQINEIIPRKIYLQFIKMFVAWFNRDIYKYIPAENLREIYGISLSFSDKYDYIGP
                     KYYRILNYHAAHDIGHALTDFQLVGCTSFAANDSLTDDHKLLIGRNFDFYMGDDFARN
                     KLLLFVKPDSGFSFVSYSWAGLTGVVSGMNEKGLTVTINAAKSDIPTTAKDPISLLAR
                     EILQHASNIKQAIAIAEKRETFVSESLLIGSAQDSTAALIEKTPTRMDVYHSPGELLV
                     CSNHYQGAELKNDPHNISNIELSDSKFRFDRITELLKENYPLSITSAVSVLRNKFGMH
                     NENIGYGNPKAINQLIAHHSILFKPQSSDFWVSTMPYQEGVYVHYNLTDVLQGKIPDS
                     DSLNIPESDFVKSNDLKKFEAFKIIKQRIYKHNTFGVNLHLTEAEIKQYIDNNPMSYV
                     TFMSLGDYFYHEKNYAMATLYYNEALKREVASKAEEEKIQKMIESCTEKSK"
     gene            complement(8832..9716)
                     /locus_tag="PKU82_04800"
     CDS             complement(8832..9716)
                     /locus_tag="PKU82_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ82227.1"
                     /translation="MSNQLFEIKRILIPYDFSETAALSLEHAVFMAKLLKADIYLLHI
                     LESVSFTSAFGAAFGNVDKKLESESNTKLDEIGKQIHMNNGITVKSITEVGRIYRKIV
                     DVAKRENIDIIIMGTHGVSGYKKFNVGTNTSKVVEEAACPVISVQTHSKKIGFKKIVL
                     PIDNSPQSRQKVSYALAVARSYGSHICIAGLIDFSNEDNKRMFKLKVDQVEEWLLQHG
                     VSVEKKFVEGDNLAKMTMQTAEELDADLIVIMTEQEPSITGFFLGTYATQVVNHSKIP
                     VMAVHPSESDGSTITVGY"
     gene            complement(9729..10616)
                     /locus_tag="PKU82_04805"
     CDS             complement(9729..10616)
                     /locus_tag="PKU82_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ82228.1"
                     /translation="MNQEKKVIVTPIDFSEQSLIALSQTYNLARLTGSHIRLLHVIDQ
                     DFISHLANSIFSKENYADQVRNDIERRLDELATKVHNEERVSTSTNIRTGKIYDEIVS
                     EANDVDAAFIVMGTQGASTIKKKFLGSNTSRVIKEALCPVITIKGHEHLKGCKDIALP
                     LDLTKETREKVNYAIDMAKLFGAVVHIFSVLETNDEFLVNKLERQLQQVKQFIIESGV
                     ECQGELIPQSDSNVSLSIIEYARKKECDLIIIMTQGETNITDLFIGTAAQEVINNSEI
                     PVLCIRPMVRKDTTQYVLS"
     gene            10783..11661
                     /locus_tag="PKU82_04810"
     CDS             10783..11661
                     /locus_tag="PKU82_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOZ82229.1"
                     /translation="MNQIPESELILNPDGSVYHLNLLPEDISDVVINVGDPDRVSKVS
                     AFFDSIEVKKQKREFVTHTGHYKGRRISVISTGIGTDNIDIVYNELDALVNIDLKTRT
                     VKSNLTALNLIRIGTSGALQPEIPVDSFVFSTFGLGLDGLLNFYQYTNTPEEQAIIDS
                     FRKHYPNNCKLPESYLTRSSHKLEQALSEGMHKGITASCSGFYAPQGRVLRYQLARTD
                     MIEKLNSFRHDEHKVTNFEMETGAMYGLAKLLGHHCCAVNLIVANRIAQQFSKKYEER
                     MHQLIQTVLDRISAQL"
     gene            11674..12585
                     /gene="gldA"
                     /locus_tag="PKU82_04815"
     CDS             11674..12585
                     /gene="gldA"
                     /locus_tag="PKU82_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOZ82230.1"
                     /translation="MSVSVKNVTKLYGKQRALDNVSFEINTGQVVGLLGPNGAGKSTL
                     MKIISCFIPQNEGKVSVCGFDTLSQSLEVRRNVGYLPEHNPLYPDMFVKEYLQFVGGI
                     YLPAKEAAKRADSMIALTGLTPEYKKKIGALSKGYRQRVGIAQALIHDPAVLILDEPT
                     SGLDPNQLVEIRALIQSIGKQKTVILSTHIMQEVEAICNRVIIINKGKIVADDDTGML
                     RQKAKPVHEITVEFDGKIDAKWLQSIPTVSKVIHHSENTWKVNYSGDDDVRKAVFTMA
                     VSHNVAVLSMQKEESRLEDVFQLLTQG"
     gene            12787..13332
                     /locus_tag="PKU82_04820"
     CDS             12787..13332
                     /locus_tag="PKU82_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOZ82231.1"
                     /translation="MTKNSSEITIIPQEVIINKIYFLRNEKVMLDRDLAELYGVKAIR
                     LREQVKRNLTKFPSHFMFQLTEQEVEIMVSQNAIPSKQHLGGTFPYAFTEHGVLMLAN
                     VLKSERAIQVSIKIIELFVKLREMLVTHTELKLEIERIKKKMDNHDKNIEIVFRYFDE
                     LMEQKINPRKKIGFKLPRKKK"
     gene            complement(13431..14714)
                     /gene="tyrS"
                     /locus_tag="PKU82_04825"
     CDS             complement(13431..14714)
                     /gene="tyrS"
                     /locus_tag="PKU82_04825"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOZ82232.1"
                     /translation="MGFIDELRWRGMLQDIMPGTEELLNKETVTGYIGFDPTSDSLGV
                     GNLVQVTTLMHFQRAGHKPIALVGGATGMVGDPSGKSAERNMLNEETLQHNLNCQKKQ
                     LEKFLDFDCGKNSAEIVNNYDWFRDVKFLDFIRDVGKHITVNYMLAKDSVKNRLENGM
                     TFMEMAYQLVQGYDFYHLYYAKNCKLQMGGSDQWGNIVTGTELLRRKGRVEDAFALTT
                     PLIKKADGTKFGKTESGNLWLDRNKTSPYAFYQFWLNASDEDAKNYIRIFSLKNQQEI
                     EAIESEHLNAPHLRLLQKTLANEITTRVHSQQDCDRAIEISEVFFNGTDKELAQLTES
                     DLQDVFNGLGKNAFEVELQKIQAGIQALDLFAAETTIFTSKGEARKMITANGVSLNKL
                     KFSDPSQIINKEQLIANKFLLIQKGKKNFFLIKAV"
     gene            14786..15079
                     /locus_tag="PKU82_04830"
     CDS             14786..15079
                     /locus_tag="PKU82_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82233.1"
                     /translation="MQPFYLYHRTTRLNIINMAHKQIIIPERFLNYLYHAFKNISNPE
                     NHRVKLITRDGQKYRNLQVINGCVLLIERSAPVTSNDIEILQLDMDFVPVKVK"
     gene            complement(15144..17279)
                     /locus_tag="PKU82_04835"
     CDS             complement(15144..17279)
                     /locus_tag="PKU82_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOZ82234.1"
                     /translation="MRHLSHIPVLRILIPFVAGIIVGIFMAVPASLAIALLSSGFIGV
                     LVFHFSKYFSQHYKLRYLFGIVLHLFLFSSGVLLTQLKTKLYDPYHYSQFESAEAFLL
                     RITEPYHIKQKSLKAEAEIEAVFENNKWQPCRGKTLLYFASDAAAKALQYADRIVTNA
                     KPQPIAPPQNPGSFNYKRFLGFHQIYHQFYLKSGRWAAAGKGFSNILLSTAFDLRKKF
                     LNEIQGQILVPQEVAVCSALLIGFNDLLDDDLLSAYASSGALHVLSVSGLHVGLIFVT
                     LQRLIFIPENKKYLNLFKQLLILVLIWFYAMLTGLSPSVLRSATMISIIIIAKQLGRD
                     NYMLNTTLASALLLLLINPFSITEVGFQLSYLAVIGIVYLHQRLVTVLYPTSMLMNQA
                     WQLTSVSLCAQLVTFPLGILYFNQFPNLFLVSNLLVIPISTGIIWVSVGATIISALPY
                     MSSLVFYSWKITFALTWLMNQLLLFFENLPFALISGLYIDIAETWIIYGIIAFVVWFL
                     LYNYRNALYGALTLTIVFLLAQLWHEWNILQQKNMTVYALKNSTAIDLAQARKRLVIA
                     PQQIIEDKSLLRFNINRYWYETGKREMLHIPFYQQPAENKLKEIFNSKIVGNVATIQD
                     GFIQMDSKLFFIPGNWLPRKTPLSKIKVDGLIVTSRFRNKLPTLFKYFDSDLLIIDLS
                     SRTKVDAGKLPVSLQIWDCRKQGAAQVEI"
     gene            complement(17413..17643)
                     /locus_tag="PKU82_04840"
     CDS             complement(17413..17643)
                     /locus_tag="PKU82_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82235.1"
                     /translation="MLSKFIAAIAQTTEQQYMERYWNYRDRYKKWFVKIGREAGESIN
                     LTENDPKGGGGALWTSQPLKVDSFLTGFNCSK"
     gene            complement(17720..18382)
                     /locus_tag="PKU82_04845"
     CDS             complement(17720..18382)
                     /locus_tag="PKU82_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82236.1"
                     /translation="MKLHACFYLLAAVIALIYGCKKDEPDEPNVSYEDIKPVKDYVYF
                     QLGTYWIYEDNYGNVDSMYVYSAGEGIDTVRKSNGDIEGIYRFFQSKIHSVYDDYNYY
                     IGYNSGWTSFHPTRHQVFMTKTKPGDYVGQIILMEYPPVIGNILYGEKNSIVKIENYY
                     QIYNIGLVNYVSVIKMSNSIDIINNDQRTNYLIAKNVGIIQEELVDSNNVWKVIRYNI
                     VQ"
     gene            complement(18596..19258)
                     /locus_tag="PKU82_04850"
     CDS             complement(18596..19258)
                     /locus_tag="PKU82_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82237.1"
                     /translation="MKLHACFYLLAAVIALIYGCKKDEPDEPNVSYEDIKPVKDYVYF
                     QLGTYWIYEDNYGNVDSMYVYSAGEGIDTVRKSNGDIEGIYRFFQSKIHSVYDDYNYY
                     IGYNSGWTSFHPTQHKVFMTKTKPGDYVGQIFLTLYPFKIGDWRYWNDSKTTTIDYFQ
                     NYGIDNNSFVSVGKVNVFNDITNNYRQTTNYYIAKNVGIIQEELIDSSNVWKLVRYNI
                     VQ"
BASE COUNT         6319 a         3693 c         3386 g         6398 t
ORIGIN      
        1 tgtttttggg cagacctctg taagctctta tcaagggcat gcctgcttca tcgggtttat
       61 tttctcccaa tttcttttta gcatcggcaa ttacagtgtt gatataattt ctttgtgcat
      121 taaacagtat ttcaactttg ggtttcaatg caaaatattc ctgagtgtcg cctttaggtg
      181 ttggacctga aataatcaag ggtgttcttg catcatcaac caatacacta tccacctcat
      241 ctacaattgc aaaatgatgt ggtcgttgta ccatttcatg agcatgacgc gtcatattat
      301 cacgcagata atcgaaacca aattcattat tagttccgta ggtaatatca gctaaatatg
      361 ccttacgtct ttcttcagtg ttgggttgat gtttatcaat acaatcaaca gaaagtccta
      421 aaaattcaaa taacggacca ttccactcag catcacgctt tgcaagatag tcgttaacag
      481 taacaacgtg cacacctgca cctgccaagg cgttgagata aatgggtaat gttgcaacaa
      541 gcgtttttcc ctcaccggtt gccatctctg caatttttcc ctgatgcaaa acagagccac
      601 ctataagctg cacatcataa tgaatcatgt tccagagaac cttacttcct gctgctgtcc
      661 aggtattgct gtatattgcc ttgccatctt ctattctgat accatcgcgt ttagttacca
      721 gatttttatc attgtcattg gcttctactg ccagctcggc attctcagta aaacgtttgg
      781 caacttcgcg gacaagtgca aatgcttcag gctgaatttc gttcagcacc tcttctgtct
      841 tcttatttct ctctttcttt aaagcatcaa tgcggttgta aatttcttct ttttggttgg
      901 catccatttc aggatcggct tcagcttctt cgttaagctg agtaagttct gtatcaatct
      961 ctgaaagata gtcctgtatg cgcgtacgaa attcatcggt tttattgcgt aattcatcat
     1021 tgctgagtga tgacatgttt tcggctactg catttactgc ttcaattaca ggtgacagtg
     1081 ctttgtaatc gctttcggcc ttactgccaa aaattttgga aatagtttta ttgataaaat
     1141 caatcattgt tgctgattat aatgtgctaa aaataaaagg tgacaaaagt aacgtaaagt
     1201 ggctgcaatt caaagagatt tgcaaaggat gattgcctaa aaactatgct ttaaatgatt
     1261 ggtgcatgaa tttagaaccc tcatcagcaa actgttgtca acagaaatgc caaaaagtat
     1321 ttggcgacat aatgacataa aagctttcta atcctgctgt tgtgcaggtt tttgtatcag
     1381 tttccactga cgatttggtg ctgtaaaaaa cagatgaacc ttttcttcaa aatcttttaa
     1441 agaagaagga gattcgggca caccatcaaa aatgcgtttt cttttaccat tcaaatttac
     1501 ggtgacgata gtagcagggt aatctgcaat tccgggatta taaaaactat cgggcatagc
     1561 aataagtcct gccttctttg catcttcaac aaaattattc aactcctgtt tatcaatctg
     1621 tgtagcataa ataccgaggc tatccataaa tccttttctg ttgatgactg cataaccgct
     1681 cttatataat gatgcatcat aatatgggca tcggccaaag catggaaaac gactcatgct
     1741 gatgagtaaa ctatctgctg aattattttc agaaactttt tttgaagtgt tgcatgaaaa
     1801 gatagctgat aaaagtagta ttgttatagg aacaaaaaat aaattggcct gtttcattct
     1861 gcaaatgtga aaataacatt tgcctttgtc aaataaatgt gtgaaatttt gtgcgtagtt
     1921 acatccttag taaaatcaat aaaaatttaa ggaaatgaaa agtataaaaa ttatgggagt
     1981 gctattactc tctatcgcaa cagtggcatt acttacatca atgaaaaaag gtagtggccg
     2041 ttacaaagta aaaaccaaag gagaaaaagg atgtgttttc cgttctgttt ttgaagcaga
     2101 aggtattgac tcaaatttta tttattcaac accggtaaaa gttaaagaat ctatgacatc
     2161 tgcattggag tggattagca aagcacagtt aaaagatggt ggctggggag caggctcaca
     2221 tgccagacag tcggtttttg acccacatgc tgtaagttct gaccctgcca caacagctat
     2281 ggttgccatg gccttgcttc gcacaggaag cacacctaca acaggaaaat atgctaccaa
     2341 tctttcttta gcaacagaat atttattgaa gcaaatagag tctgcagatg cacaaacttt
     2401 aaatattacc aatcaaactg caacacaacc acaggttaaa cttgggcaaa atatagatgt
     2461 agttcttgca acacaatacc tgaccaatct gatggattac atatctaaca atcagacatt
     2521 gaaaacgaga gtaagtaaga gtcttgataa atgtgttgta aaaatacaac atgcccagag
     2581 cgaaaatgga agtataaaag gtgcaggatg ggcaggtgtt ttacaatcat cgtttgcaac
     2641 taatgcatta gaaagtgcaa aagccgcagg agcaaatgtt gacgaagaaa aacttgagcg
     2701 atataaaaat tatcagaaag gtaactatga tgcaaagaca aattccgtag ctacaggtga
     2761 tggtgccgga gtaatgttat acagtatttc gggcactgct cgcagcacag cacgcgaggc
     2821 aagtattgca aaagataaaa tatcgagagc aaaaaaagaa ggcaagctag cgcctacttc
     2881 aactgtaaca agcgaaagtc tgcaggctgc cggcatgagt gaggctgatg cttacaaata
     2941 tgctgctgct tacgaaatta atcaggctgc aagtaagcag gcacagcgta atgatgtgat
     3001 gaatggattt ggcagcaatg gtggtgaaga gtttctttca tatctgcaaa ccggtgaagg
     3061 tttaataatc agtaaggata aagagtggca taaatggtat gataatgtta gcggaagatt
     3121 gttgagcatc cagaataatg atggcagttg gaatggacat cactgtatta caagtccggt
     3181 tttttgtaca gcaacttcat tacttattct atctgttaat aatgatattg aaaaattggt
     3241 ttcgctgaat aaataaaaga attacgggag tgctgcatgg cagcactccc gtaatttttt
     3301 aaacttttac tttacacttg agtatagtat gaaaactttc gccaattaat ggaaatgtct
     3361 caaccacttc aagccctgac ttttccacca aacctgtcat tgcttttaca ctgtacattt
     3421 tactgttgcc gtttgcaagc gctgtaaaat acagtgatgt tgcagtaagg caataagaag
     3481 cggcttcaaa cttttgatta tcaatgaatg gttcgagaat aaaaacagta gtgtgaggtg
     3541 atgctgcctg tctgacattc tcaagaatag agacaatttc atcttcacca aaacaatcta
     3601 aaaactgact catccaaaca acatcggcac ctttaggaat tttctgactt ttatcaagca
     3661 ggttgatttc aaaatatgaa atgcgctctg ttagatttct tttctctgca ttggcttttg
     3721 ccactgcaat ttgttgtggc aggtcaagaa tttttacatg cacatcagga ttgaattcac
     3781 aggaagcaaa tgcccatttg ccggtattgc caccaatatc aaaaatagtt gatggatttt
     3841 ctttaaacac aatgttgagc gcatcacgga atgcttcatc gctataaaaa tgatcaaact
     3901 caaaccacga ttttttaata tgttctggca atgatgataa agcaggataa atagttggcc
     3961 agttgccgag cgtttttaat ccttcgggtt taccattttg aatagactct gtcagaaatt
     4021 tgaatccatc ataacaaaca tcctgtgtaa agtttatgtt aacacgagtc atttcatcag
     4081 aattcaaaaa atagccgagc atagtaactg ttacagtact gtcgtctaaa tattttacaa
     4141 tatctgcatt ggctgccatt tcgagcaaaa cttttactcc ataaatactt acgttagtct
     4201 tttcacttat ctcttttaca gtaattcctt ttctattttt acaaatcaat ggtaaaatgc
     4261 ctaagcgttg catggcaacc actacctgaa aaactaccgg agcaaacgct aatttttgcg
     4321 cctcgttttt tgcttcaaat gctgataatt ctcttttttc tgtcattgta ttttctgctg
     4381 aacccatctt tctattcctg aactattttg tgtgaatatt ttttacgcaa tctttttttc
     4441 ttcaggtctt cggcatgcca ttttatgttt ttacctaaag ttcctgcaaa gcgttgttta
     4501 aacgtttctt tctcaggcaa agaaataaaa ttttctactc tctgcaattt tccatcaata
     4561 tcaggctcac tatgcggcag taaacgaagt gttttgtaca taatgggcag ataggagatt
     4621 ggcagactaa agcgatggat aaaacttacc attgaacggg tttgaacggg gtctgttgcc
     4681 aaggcaattt ttttaaatcc taacttcttt gccaaacgat atgaataata cacattttct
     4741 acgctgtgct ctgctttgtc ttctacatag atatgttctt cgggtatgcc catggctata
     4801 gcaaatgctt tcattgtctt tccttcgaca aagggtgagt aaacagcatt gcccgaaaaa
     4861 ataatatttt tagtgtagcc attctgatat agaaattttg cccaaatcat tctcatttgt
     4921 actacaggtg gccagggctg accttcaaaa tatggaaacc cgggaacaat aattgcatca
     4981 taaggttttg ttgcaattgc tttgctgtat gctttatcct tagataaaaa aattcctctg
     5041 caagaagaaa gaaaaaaaga aagcaggcaa aaccatagaa tatgcctgct taaatgaaag
     5101 cggaaaattt tattcaccta tttacttgaa tttattcttc cagattttgt atttcttaga
     5161 ctcaatttct tttttaagct tggttgcagg cgcaatccag taattcttca atccaaaacc
     5221 acctgctttc tcttctacct tttctgtgta aagcattttg ttatttgaca ggtcaacaaa
     5281 tgttacccag ataacagctt tctctaaagt cttattgaaa ctttcaacta cataaaccaa
     5341 accaattgca tctttctcct tcgatttata atgtgaaact gcttttttta catcatcttc
     5401 tccaattgaa tagcttccat tcatcactct ttcgtgtaca ttaattttac tgttgatatc
     5461 ctgaactaca tcaacattgg tttcgtactt atcgttagta agcttaaagg cactttgcaa
     5521 actgaatttt tcatattctg caacaaaagt attattccat gccgaaagaa acttgtcttt
     5581 gatggcagca gggtcggtaa atccgtctgt tccgataaat cgcgcttgtg aaaagtcata
     5641 acccataaaa atgagtttca cattgttctt aaagagttca ttcatttttt cctgtgaaaa
     5701 tgcattgagc atgatgaatg aaaagattaa agtaaggtag gtctttttca ttttctattt
     5761 tattaaaatt aatttttcaa atgtaaatta ttttttcgga tttacgttta tctgtcttaa
     5821 aaactttttg ctgtaactct tcagtatcaa tatacctgaa tgccggagtt actcttatct
     5881 tggacttgaa taatgaagaa atttcatgtt caaatgcatc agaatgtaac tcttttgaca
     5941 aaagaattgt tattgaatca ttattaaatt catttcgttt gacttcaacc tgaaacaatt
     6001 caatttgcgg catcatattg agtacattaa aaattatttg tggaaacaat gtagtgcctt
     6061 taaacttaat catctgttgc tttctaccca taacaggacc taagcgggta gtatatcggc
     6121 cacatttaca cagatcataa tgagcgtggc ataaatcacc ggtcttaaat ctgattaaag
     6181 gcataccttc tacaccaagg gttgtgacaa caacttcgcc caattcgcca ttacgtacct
     6241 gaagaccatt atcgtcaaca acttcaagaa ttaataactc aggatgtgca tgacctccat
     6301 tgccatagcc acattctgta aatgcagaac tcatctctgt tgatgcatag gttgaataca
     6361 atttcacatc ccaaagcgaa gtgattcttt ttcctagttc attcaatgaa aaatctgcat
     6421 tacgaacagg ctctccaaga caaactattt tttttaccga ggttgattga aaattaattt
     6481 ttctttcagc tgcatattgc aacagttgtg gaataaatga tggtatggct attaatgttg
     6541 ttggcttgaa acgttgaata taatcccact gcgcctgagg tgttcccggc cctactctga
     6601 tactggcagc acccaacttt ctgataccta aaaaataggc taatcctgcc ataaacaatt
     6661 tgtcaatggt agtcatcatc tgaaaaatat ctgcactgct gccatcagca cattgcaagg
     6721 atatggcctc gttatatgcc aatctgtcta agtctgccga tgtcagatag aatgtaacag
     6781 gatcgctcaa agtacctgat gtagtcacat aatctgcaat aaaactttta tcaacacata
     6841 aaaaagaatc attttgtgtt gccaggtcat ccttagttgt aaatggcaat tcgttcagat
     6901 tgtcgagagt aatgctgtca aacttaatgt tgtgttgcct gaagacttct ttataataag
     6961 ctgatttggc attaacgtat tgcagcagtt cagaaagctt ttgttgctgc atttttcgaa
     7021 tggcatcaag acctgcaaat tctatttcac caagctgttt cattattttg atttttcagt
     7081 acaggattct atcatctttt gaattttttc ctcctctgcc tttgatgcta cttcacgttt
     7141 taatgcttca ttgtaataaa gtgtggccat tgcataattt ttctcatgat aaaaataatc
     7201 acccaagctc ataaaggtaa cgtaactcat agggttatta tcgatatact gttttatttc
     7261 ggcttcagtc agatgcagat ttacaccaaa tgtgttgtgt ttgtagattc gttgtttgat
     7321 aattttaaaa gcttcaaact tctttaaatc gttagacttt acaaaatcac tttccggaat
     7381 attcagagag tcagaatcgg gaattttacc ctgtaaaaca tcagttaaat tatagtgaac
     7441 ataaacacct tcctgataag gcattgtaga aacccaaaaa tcgctgcttt gtggtttgaa
     7501 taaaatactg tgatgtgcta taagctgatt aattgccttt gggttaccgt agccaatatt
     7561 ttcattgtgc attccaaatt tatttcgcag aacagaaact gctgatgtta tacttaatgg
     7621 ataattctcc tttagcaatt ctgttattct gtcaaagcgg aatttactgt cgctaagttc
     7681 aatatttgaa atgttgtgtg gatcattttt caattcagca ccctgatagt gattggaaca
     7741 aaccaataat tcaccgggtg aatgatatac atccattctt gttggtgttt tttctatcaa
     7801 ggcagccgta ctatcctgcg ctgaaccaat aagcaacgat tctgaaacaa aagtttcgcg
     7861 cttttctgca atagcaatcg cttgctttat gtttgatgca tgttgtaaaa tttcgcgtgc
     7921 taaaagtgaa ataggatctt ttgcagttgt tgggatatca gactttgcag cattgatggt
     7981 aacagtaagt cctttttcat tcataccgga aacaactcct gtaagtcctg cccacgaata
     8041 agaaacaaaa gaaaaaccac tatcaggctt tacaaaaagt aagagtttgt ttcgtgcaaa
     8101 atcgtccccc atgtaaaaat caaaatttct gccgattaat aatttatgat cgtcagtaag
     8161 actgtcgttg gcagcaaatg aagtacagcc taccaattga aaatcggtaa gagcatgacc
     8221 aatgtcgtgt gctgcatgat aattgaggat gcgataatat ttaggtccta tgtaatcgta
     8281 cttgtcagaa aaagaaagtg aaataccata aatctcacgc agattttcag ccggaatata
     8341 tttgtaaata tcgcgattga accatgctac aaacatcttg ataaactgaa gatagatttt
     8401 acgtggaatt atttcattga tctgatcaac aaaatattct tcttgttttt caattaactc
     8461 ttttgcaagc acaccataaa ctacacctct ttcatagtca ttaccttcaa tatacatttc
     8521 ccagatgcct tgctgatttt tgcgcaacca gttgttgcca acacgatagc tgtcaactcc
     8581 tatttgaatg cgatgaaggt catctggttt aagtttacta cttatagaag gatcgggaat
     8641 ctgaatcctc cataaaaaca aaagaaataa tataataagt attcctgcag tccaaagtaa
     8701 aaacttaaaa ataaaacgca gactaccttt aaatgatggc atttatagta attaaaattc
     8761 tgattggcga aggtaataaa aacatggggc gctcttcaag cgccccatgt gtaaatcatg
     8821 aaatttgttt tttaatatcc tacagtaatt gtactgccgt ccgattctga aggatgcact
     8881 gccataacag gaattttgct gtggttaaca acctgtgttg cataggtgcc cagaaaaaat
     8941 cctgtaatac taggttcttg ttctgtcatt attacaatta aatcggcatc caactcttct
     9001 gctgtctgca tagtcatttt tgctaggtta tcaccttcaa caaatttttt ctcaacactt
     9061 acaccatgtt gtagaagcca ttcctcaacc tgatcaactt taagtttaaa catccgctta
     9121 ttgtcttcat tgctgaagtc tattaatccg gcaatacaaa tatgcgagcc ataagagcgg
     9181 gctactgcta atgcatagct taccttctga cgtgactgcg gtgaattatc aattggtaaa
     9241 acaattttct taaagccgat ttttttgctg tgtgtctgta cagaaatcac cggacatgca
     9301 gcttcttcaa ctaccttact ggtgttagtt ccaacattaa acttcttata tcctgaaaca
     9361 ccatgtgttc ccataattat aatgtcaatg ttctcacgtt tggcaacatc aacaattttg
     9421 cgataaatcc ttccaacctc agtaatagat tttacggtta ttccgttgtt catgtggatt
     9481 tgtttgccga tttcatcgag tttagtattc gattcagact ctaacttttt atctacattt
     9541 ccaaacgctg caccaaatgc cgaagtaaaa gatacagatt caagaatatg tagcagataa
     9601 atatctgcct ttaataattt ggccatgaaa actgcatgct ccaagctcaa cgctgctgtt
     9661 tctgaaaaat cataaggaat taaaattctc ttgatttcaa ataattgatt actcatagga
     9721 aaatggattt atgatagtac gtattgtgta gtgtcttttc taaccattgg tcggatacat
     9781 aaaacaggaa tctctgagtt attgataact tcctgtgctg ctgtgccgat aaataaatct
     9841 gtaatattgg tttctccctg tgtcatgata ataattaaat cacactcttt ctttcttgcg
     9901 tattcgatta tacttaatga tacatttgaa tcactttgtg gaatcaattc accttgacat
     9961 tctactccgg attcaataat gaactgcttt acctgctgca attgtcgttc cagtttattg
    10021 actaaaaact catcattggt ttcgagcact gaaaaaatgt gcactacagc accaaaaagt
    10081 ttagccatgt caatggcata attcaccttt tcgcgtgttt cttttgtaag atccaatgga
    10141 agagcaatat ctttacaccc cttgaggtgc tcatgacctt ttatggtaat gacggggcat
    10201 aaagcttctt taattactct tgaagtatta gagccaagaa atttcttctt tattgtgctt
    10261 gccccttgtg ttcccatcac tataaatgca gcatcaacgt cattggcttc agatacaatt
    10321 tcatcataaa tttttcctgt tctgatgtta gtagaggtcg aaactctttc ttcattatgc
    10381 actttagttg ccaactcatc aagtctgcgt tctatatcat ttcgaacctg atcggcatag
    10441 ttttctttag aaaaaatact gttggctaaa tgggaaataa aatcctgatc aattacatgt
    10501 aaaagcctga tatggctgcc ggtcagtcgt gcaagattat aagtctgtga aagggcaatg
    10561 agtgattgtt ctgaaaaatc tatcggggta actataacct tcttctcttg gttcataaaa
    10621 aacaggtgta ttaactatgc aaatataaaa aaaggaaata tcaaattgaa taaaaaatga
    10681 cctccaacag attatttaac tgtaaattac atgaacaggt aggtcttttg acctgatgct
    10741 ttaatatatc ttaccttagc acagaaatta tttacatttg ttatgaatca aatccccgaa
    10801 tcagagctta ttctaaaccc cgatggaagt gtttaccatc ttaatcttct tcccgaagat
    10861 atttctgatg tagttatcaa tgttggcgac cccgatcgcg tatcaaaagt gagtgccttt
    10921 tttgacagca ttgaagtaaa aaagcaaaaa cgagagtttg taacccatac cggtcattat
    10981 aagggccgaa gaatatctgt tatttcaaca ggtattggca cagataatat agacattgtc
    11041 tataatgagc ttgatgcttt agtgaatatt gatcttaaaa caagaactgt aaaatccaat
    11101 ttaacagcac ttaatcttat tcgtattggc acttccggtg cccttcaacc agaaattcct
    11161 gttgattcct ttgtgttttc gacattcggt ttaggattag atggtttgct taacttttat
    11221 caatacacaa acaccccaga agagcaggct ataattgata gtttcagaaa acattatcca
    11281 aataactgca aactaccgga atcatatctg acacgaagct cacataaatt agaacaagca
    11341 ctttcagaag gtatgcataa aggcattact gcatcttgtt caggttttta tgcaccacaa
    11401 ggaagagtgt tacgctatca attagcaaga actgatatga ttgaaaaatt aaattcattc
    11461 cgccatgatg agcataaggt tactaatttt gaaatggaaa caggagccat gtatggattg
    11521 gcaaaattat taggacatca ttgctgtgcc gtaaatctga ttgtggcaaa cagaattgcg
    11581 caacagtttt caaaaaaata tgaggagcgt atgcatcaat taattcaaac tgtgttggac
    11641 agaattagtg ctcaacttta aatcttccag agtatgtctg tttcagtaaa aaatgtaaca
    11701 aaactttatg gcaagcaacg tgcattggat aatgtaagtt ttgaaatcaa tacaggtcag
    11761 gttgttggtt tgcttgggcc aaatggcgca ggtaaatcaa cgcttatgaa aattatttcc
    11821 tgttttattc cgcagaatga agggaaggtt tctgtttgcg gttttgatac actttcacaa
    11881 tcacttgagg ttagacgaaa tgttggatac cttccagagc ataatccact ttatcctgac
    11941 atgtttgtta aagagtatct acagtttgtg ggtggaattt atttgcctgc aaaagaagct
    12001 gcaaagcgtg ccgactctat gattgccctt acaggtttaa caccggaata taaaaagaaa
    12061 attggcgcac tctcaaaagg ctacagacaa agagttggca ttgcacaagc tttaattcac
    12121 gatcctgcag tactcattct tgatgaacca acttccgggt tagaccccaa tcagttggta
    12181 gaaattcgtg cattgattca atcaatagga aaacaaaaaa ccgttattct ttctacacac
    12241 attatgcagg aagttgaagc aatatgtaat cgtgtaatca taatcaacaa aggaaaaatt
    12301 gtggctgatg atgatacagg tatgcttcgt caaaaagcaa agccagtaca tgaaatcact
    12361 gttgagtttg atggaaaaat tgatgccaaa tggttgcaat ctattcctac ggtgagtaag
    12421 gttattcatc attccgaaaa tacatggaag gttaattaca gcggtgatga tgatgtgcgt
    12481 aaagctgttt tcactatggc agtcagtcat aatgtagctg tgctgtcaat gcaaaaagaa
    12541 gaaagcaggc ttgaagatgt gtttcaactg ctaacgcaag ggtaatttaa gaatttacaa
    12601 aaccaatgtt acatcagtat ctttttatta atgtcttgtt gtgatattga tatacttaat
    12661 tctgaaattt tgtaaaacat gttataaatt ttgtaatacc tatacacaga ttctgaagga
    12721 agaagtaatg tgatttttta taagtacttg ttaaacacct aaatttacat ctctgataat
    12781 acaagaatga caaaaaatag ttctgaaata acaataatac cacaggaagt aataataaat
    12841 aaaatttatt ttttaagaaa tgaaaaagtg atgcttgaca gagatttagc tgaattatat
    12901 ggtgttaaag ccatcagact acgagaacaa gtaaaacgaa atctaacaaa atttccttcc
    12961 cattttatgt ttcagttaac tgaacaagag gttgaaatta tggtatcgca aaatgcgata
    13021 ccttcaaaac aacatttagg tggtactttt ccttatgcat ttacagaaca tggtgtacta
    13081 atgttagcta atgtcttaaa gagtgagaga gctatacagg taagtattaa aataattgag
    13141 ttgtttgtga aacttcgtga aatgttggtt acacatactg aactgaagtt agaaatagaa
    13201 cggataaaaa agaaaatgga taatcatgat aagaatattg aaattgtgtt tagatatttt
    13261 gatgaattga tggaacaaaa aataaatccc agaaagaaaa taggatttaa actaccaagg
    13321 aaaaagaaat aaccacatat aaatcctgcc tgttaaaacc aaataacaga taatttaaaa
    13381 agattgttgc taaatgctta gaaaagtaac aaatacttat gctaagaagt ttaaacagcc
    13441 ttaatcagga aaaaattctt cttacctttc tgaatcagta aaaacttatt tgcaatcagt
    13501 tgttctttat tgataatttg tgaagggtca gaaaatttca atttattcaa tgagacacca
    13561 tttgcagtaa tcatttttct tgcttcacct ttacttgtaa aaattgttgt ctctgctgca
    13621 aataaatcaa gtgcctgaat acctgcctga attttttgca actcaacttc aaatgcattt
    13681 ttgcctaatc cgttaaaaac atcctgcaaa tcagactctg ttagttgcgc aagttcttta
    13741 tctgttccat taaaaaatac ttcagaaatt tctatagctc tgtcacaatc ctgttgtgaa
    13801 tgcacacgag tagttatttc attagctaat gttttctgaa gcaatctcaa atgaggtgca
    13861 tttaaatgtt cagactctat tgcttctatc tcctgctgat tctttaaaga aaatatacgg
    13921 atataatttt ttgcatcttc atcgcttgca ttcagccaga actgataaaa agcataagga
    13981 gatgttttat ttctgtcaag ccacaaattt ccactctctg tcttaccgaa tttagtacca
    14041 tctgcttttt ttatcagtgg tgttgttaaa gcaaatgcat cttcaacacg gccttttctg
    14101 cgaagcaatt cagttcctgt aacaatattt ccccactgat cgctgccacc catttgcagc
    14161 ttacagtttt ttgcataata caaatgataa aaatcgtagc cctgcactaa ctgatatgcc
    14221 atttccataa atgtcattcc attctctaat ctgtttttta cagaatcttt tgccagcata
    14281 taattaacag taatgtgttt ccccacatca cgaataaaat caagaaactt tacatccctg
    14341 aaccaatcat aattattaac tatttctgct gaattttttc cacaatcaaa atccagaaat
    14401 ttttctaatt gttttttctg acaatttaaa ttgtgctgca gtgtttcttc gtttaacata
    14461 tttctttcag cactctttcc gcttggatcg ccaaccatac ctgttgcacc gccaaccaag
    14521 gcaattggtt tatgtcctgc tctttgaaaa tgcatcaatg tagtaacctg cactaaattg
    14581 cctacgccta atgaatcgct ggtaggatca aatcctatat atccggtaac cgtttcttta
    14641 ttcaataatt cctctgtacc gggcataata tcctgcaaca tgccgcgcca tcttagttcg
    14701 tcaataaatc ccatatctga tttttagtgc cggcaaagat aatcatacag tgctttactg
    14761 ctatcagtta caaagggtaa aattcatgca acctttttat ctgtatcacc gtacaacacg
    14821 cttaaacatt atcaatatgg cacataagca aatcattatc cccgaaagat ttctaaacta
    14881 tttatatcat gcttttaaaa atataagcaa tcctgaaaat cacagagtaa aacttattac
    14941 tcgtgacgga cagaagtatc gaaatctgca ggttattaat ggctgtgtat tgttaattga
    15001 aagatctgca cctgttacct caaacgatat tgagattctt caacttgata tggattttgt
    15061 tcctgtaaaa gttaaataat atttgatgtt ggcacagccg gtagttcctt ttggggattg
    15121 ccggtttttt atttgctgct ccctcaaatt tctacttgtg ctgcgccttg ttttctgcaa
    15181 tcccagattt gtaaactgac aggcaattta cctgcatcaa cttttgttcg gctgcttaaa
    15241 tcaataatca acaaatcaga atcaaaatat ttaaagagcg taggcagttt atttctgaaa
    15301 cgtgaggtaa caatcaatcc atcaacttta attttactta atggtgtttt tcgcggcagc
    15361 cagttacccg gaataaaaaa taatttacta tccatctgaa taaatccatc ctgaatagta
    15421 gcaacatttc ctacaatctt actgttaaaa atttctttca acttattttc tgcaggctgc
    15481 tgataaaatg ggatgtgaag catttctctt tttcctgttt cataccaata gcggttaata
    15541 ttaaaacgta acaggctttt atcttcgatt atctgttgtg gtgctatgac caacctcttt
    15601 cttgcctgtg ccagatcaat tgctgtgctg ttttttaaag catatactgt catgtttttc
    15661 tgttgcagaa tattccactc atgccaaagt tgagccaaca gaaaaactat tgttaacgtc
    15721 aatgctccat aaagtgcatt cctgtagttg taaagcaaaa accaaactac aaaagcgata
    15781 atgccataga taatccatgt ttctgcaata tcaatataca atccgctaat caatgcaaat
    15841 ggaaggtttt caaaaaacaa caataactga ttcattaacc aagtgagtgc aaaagttatt
    15901 ttccaagaat aaaatacaag tgatgacatg tagggcaatg ctgatataat tgtagcacca
    15961 acagacaccc agataattcc tgtagagata ggtatgacta aaagattaga tactaaaaac
    16021 aaattcggga actgattaaa ataaagtatt cctaacggaa atgttaccag ctgcgcacat
    16081 aatgacactg aagtcagttg ccatgcctga ttcatgagca ttgaagtagg ataaagaacc
    16141 gtaaccaatc gctgatgcag atatacaatt ccgataacag caaggtatga aagttgaaaa
    16201 ccaacttctg taattgaaaa tgggttaatc aacaacaaca ataaagcaga agccagtgta
    16261 gtgttaagca tgtagttatc acgaccaagt tgctttgcaa taatgatgat agaaatcatg
    16321 gttgctgacc gtaaaacgga aggagaaagt cctgttagca tggcataaaa ccatatcaaa
    16381 accagaatga gtaattgttt aaaaagattc agatacttct tattttcagg aataaaaatc
    16441 agcctttgca gggtcacaaa aatcaagcct acatgcaatc cggatacact caacacatgt
    16501 aatgcaccgg agcttgcata ggccgacaat aaatcatcgt caagcaaatc attaaagcca
    16561 attaacaatg cagaacagac cgctacttcc tgcggcacta aaatttgtcc ttgtatttca
    16621 ttcaaaaact ttttgcgtaa atcaaaagct gtactcaaaa gaatattaga aaatccttta
    16681 ccggcagcag cccatcgccc cgactttaaa tagaactgat ggtatatctg atgaaaacca
    16741 agaaatcttt tgtagttaaa tgaccccgga ttttgtggag gagcaattgg ctgtggcttt
    16801 gcattggtaa caatcctatc agcatactgc aatgcctttg ctgctgcatc ggatgcaaaa
    16861 tacaacaatg tttttcctct gcatggctgc catttattgt tttcaaagac agcttctatt
    16921 tctgcttctg cttttaaaga tttttgtttg atgtgatatg gctcggtgat acgaagtaaa
    16981 aaagcttcag cgctttcgaa ttgcgaatag tgataggggt cgtaaagttt cgtctttaat
    17041 tgtgtgagta aaacgcctga agagaataaa aacaaatgca atactattcc gaaaaggtag
    17101 cgaagtttat aatgctgact aaaatatttt gagaaatgaa aaactaaaac tccgataaaa
    17161 cctgaagaca acaaagcgat tgccaaagat gcaggcacag ccatgaatat gcctacaatt
    17221 ataccggcaa caaaaggtat taaaattctt aaaacaggaa tatgcgatag gtgacgcata
    17281 actgtgcggt ttggtgtaaa attaagaaaa ttaattctga attgtagaat ttaatcgaga
    17341 ataatttatc ttacttaagg ctttttacag cagggcctgc ttccgtttaa attcatcctg
    17401 caatattcta tatcatttac tacaattaaa acctgtcaaa aagctgtcca ccttcaaagg
    17461 ctggcttgtc cacaatgcgc cacctcctcc cttggggtcg ttttcggtca ggttaatgct
    17521 ttcgcccgcc tcacgcccta tttttacaaa ccatttcttg tatcggtctc tgtagttcca
    17581 gtagcgttcc atgtattgct gctctgttgt ttgtgcaata gcggcaatga attttgaaag
    17641 cacagcagca gcatggctat ggttttccag ttgattttaa ttttcatact atttggtttt
    17701 tagtggttat taataaattt tattgtacta tgttatacct tattaccttc catacattat
    17761 tgctatcaac cagttcttcc tgaataatac ctacgttttt ggcaattaaa taatttgtcc
    17821 tctgatcatt attaattata tctattgaat tcgacatttt aataacagat acataattta
    17881 ctaacccaat attatatatt tgataatagt tttcaatttt tacaatagaa tttttctcac
    17941 catataatat attaccaatg acaggaggat attccattaa aattatttgc cctacataat
    18001 ctccgggctt tgttttagtc ataaagactt gatgtctggt tggatgaaaa gatgtccagc
    18061 ctgagttata accaatataa taattgtaat catcataaac actgtggatt ttactttgaa
    18121 aaaagcggta aataccttca atatcaccat ttgattttct tacggtatca atgccttctc
    18181 cggcagaata tacatacatg ctgtcaacgt ttccgtaatt atcctcatat atccagtaag
    18241 tgccaagctg gaaataaaca taatccttaa caggcttgat gtcttcatag cttacatttg
    18301 gctcatcagg ctcatctttt ttgcagccgt aaatcaaagc aataacagca gcaagcaaat
    18361 aaaaacaggc atgcagcttc atgagtgttt tcttgaaggt gtaaatatat gaggcttgag
    18421 ttaaaacaaa tatttttaaa aattatttat gcgtatgctt tgcacaaaca gcggcaatga
    18481 attttgaacg cacagcaaca gcatggctat ggttttccag ttgattttaa ttttcatact
    18541 atttggtttt tagtggttaa atgaatctat caatagtaaa tattaaagat taggtttact
    18601 gaacaatatt atatctgact aacttccaca cattgctgct gtctatcagt tcctcttgta
    18661 tgattcctac attctttgca atataataat ttgttgtttg tctataatta ttcgtaatat
    18721 cattaaaaac attaaccttt ccaaccgaaa caaaactatt attgtcaata ccataatttt
    18781 ggaaataatc aatagtagtt gttttagaat cattccagta tctccaatca cctattttaa
    18841 agggatataa ggttaaaaaa atttgcccta cataatcccc cggctttgtt ttagtcataa
    18901 aaaccttatg ttgggttgga tgaaaagatg tccagcctga gttataacca atataataat
    18961 tgtaatcatc ataaacactg tggattttac tttgaaaaaa gcggtaaata ccttcaatat
    19021 caccatttga ttttcttacg gtatcaatgc cttctccggc agaatataca tacatgctgt
    19081 caacgtttcc gtaattatcc tcatatatcc agtaagtgcc aagctggaaa taaacataat
    19141 ccttaacagg cttgatgtct tcatagctta catttggctc atcaggctca tcttttttgc
    19201 agccgtaaat caaagcaata acagcagcaa gcaaataaaa acaggcatgc agcttcatga
    19261 gtgttttctt gaaggtgtaa atatatgagg cttgagttaa aacaaatatt tttaaaaatt
    19321 atttatgcgt atgctttgca caaacagcat ccctggcgca agtcttcgga cttgtgccca
    19381 cctatcaata aagaatgcaa tgcaataatt tagcgcaaca aggcagcaag caggttaaac
    19441 cgcactttac tttgctcaaa taatatttac aatcaataga agttctccct ggcgcaagtc
    19501 ttcggacttg tgcccacctg tcaataaaga atgctatgca ataatttagc gcaacaaggc
    19561 agcaagcagg ttaaaccgca ctttactctg ctcaaataat atttacaatc aatagaagtt
    19621 gcacatttgc ctccctgacg caagtcttcg gacttgtgcc cacctgtcaa taaagaatgc
    19681 tatgcagtaa atttagcgca acaaggcagc cagcaggtta caccgcactt tactttgctc
    19741 taataatatt tacaatcaat agaagttgca catttgcata aatgagtacc aaatac
//
LOCUS       DAOOOL010000038        19748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_211_length_19748_cov_112.099731, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000038 DAOOOL010000000
VERSION     DAOOOL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19748
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_19748_cov_112.099731"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..2527
                     /locus_tag="PKU82_04855"
     CDS             272..2527
                     /locus_tag="PKU82_04855"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ82238.1"
                     /translation="MTTKSFWSQRAMLSIRSNKRLFLGCLLAGSVGASAYLFLMPKVY
                     ESQAILKNINPDKQSTAIAAEMIRSPKTIQQSVKGMGLNVEYFVTKNFQTSECFDNKP
                     VNISYKLKDESFSQQSFSLQRDGEKTYILEYDDQAGHQQKRGSYNELLDLGSLAVTIE
                     KTPYLNLLDDKDEVSFTIYSDSRLANKIAEQNLSCSIEDGGVIKLNSQSANPAKAYRI
                     ASAVSKGYGNAGQQANESHSLANVQLINNQLDRVSQELDNAQRELSHFQSVNNAYEMP
                     QQAGAVLNTVSQLQIQKVEADMQLAALDNLSDYMRKNRNVNTISPEYGTITDIIYTET
                     YLKLNDKVAERQQLLEQGADLSKVDKEISSLKEMLAESIRNTRKKLTIRQETLAAAIV
                     SAKDRIQNLPQTEEKLQQLNRSIYLYTKLYDFLIQKRAEAMVEAPVLPPSSYILNEAK
                     MPTSPISPNAAKVWSLALLASLLFGALVTAVKPLLKIKISNRKDLQKFTDIPFIANIE
                     AGGKNHDFSESFMTLCTKILMLGNKQPVQMITITSAKAGEGKTTVAENLAKAMGKLGK
                     KALLIDMNPINPSLKNTLGNEEENSFAQVIDDDKNLHQAVQITNYENVDLLSAGYLAH
                     GVNTLLINDKTGNILADMKTLYDYVIFDTPETSNYMDAVPLMKMSDLNMFVIKANSAT
                     FDQLEYASQMKKDFQVENLFMIINGMTETMNHSGMQTSGKVKQIRRREQAEAEIRFMP
                     RMMKKAALWFY"
     gene            2538..3272
                     /locus_tag="PKU82_04860"
     CDS             2538..3272
                     /locus_tag="PKU82_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82239.1"
                     /translation="MGDKAKFLNHFFERISDIRYAAMGLTVSHPEEIAEDIIQIAINR
                     EDTRKILTVLEFSPYLKRVRLEEKPFYNFIHLKLSNGQWIQIQLIYGFVRKGIQFMNE
                     KEVLNECTINSGGIKVTSQHLNFEYVWLIHCLNKTNVPNHQHLFFSQYNRNERSAIFA
                     HIRSRYFLELNVLDELYTFHKKTYRQVYEKIIRRKENTWAKRNIRKTLYAFYSALNLI
                     KTKSIKVQFKGSGYHPFSKTERARVY"
     gene            complement(3269..3676)
                     /locus_tag="PKU82_04865"
     CDS             complement(3269..3676)
                     /locus_tag="PKU82_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOZ82240.1"
                     /translation="MNRPIRVLISKVGLDGHDRGAKVIASFLRDAGMEVIYTGLRQTP
                     QMVVNTALQEDVDVIGVSILSGAHLTVFPKIMELLKEKGMTDVLVTGGGIIPDADMKK
                     LNDMGVGKLFAPGTNTNEIVEYINQWVKQNRKD"
     gene            3802..4284
                     /locus_tag="PKU82_04870"
     CDS             3802..4284
                     /locus_tag="PKU82_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOZ82241.1"
                     /translation="MKKLVVQLFLLFSYGTTVAQDTNNNIPKPQSIIPQSGIFILHRK
                     IAIVEGPAWVTGDAAARVYCTQLKDSIGLHLAIVNAMREGTFENAINLKKINDAALGN
                     AGYRIKIKRPAIIVEANNDTGLTNALQYLFQIIPKKFFEDKNPKAKIPLSCSEITCKF
                     "
     gene            4554..5348
                     /locus_tag="PKU82_04875"
     CDS             4554..5348
                     /locus_tag="PKU82_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ82242.1"
                     /translation="MEIFGYACAILMGLSLGLIGGGGSILTVPILVYLFSIDVVTATA
                     YSLFIVGFTSLIGSFNHIRMGNVHLRTAVLFGIPSIIAVFITRAFIVPAIPQHLFNIG
                     TLQITKPIALLLLFAVIMLLASFSMIRKKKEKPDYDKVQYNYPLIIIEGFVVGTITGL
                     VGAGGGFLIVPALVLLAHLPMKKAIGSSLLIIATNSLLGFTVSLLHSEKTDWKFLLTF
                     SAIAIIGIITGSMFSKNVSNEKLKPAFGWFVLIMGVYIILKETLLK"
     gene            5447..6010
                     /locus_tag="PKU82_04880"
     CDS             5447..6010
                     /locus_tag="PKU82_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOZ82243.1"
                     /translation="MLELILKPWPWYVAGPLIGLIVPALLILGNKSFGISSSFRHVCA
                     ACMPSNIPFFKYDWKKERWNLFFVAGIMAGGFLAVNLLSASTKVDINPSLAASLNEKG
                     ITDFSGLVPADLFNWNSLFTLRGFIMMVVGGFLVGFGTRYAGGCTSGHAIMGLSTLQL
                     PSLIATCCFMAGGFIMANLILPYILSL"
     gene            6021..6533
                     /locus_tag="PKU82_04885"
     CDS             6021..6533
                     /locus_tag="PKU82_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOZ82244.1"
                     /translation="MKTEVTLEKEQNTDFETRSLDTICVNESHLEHSWEHNLKYSLMG
                     ILFGIILVKAEVVSWFRIQEMFRLQSFHMYGIIGSAVVVGALSVWLIRKFNIKTIYGE
                     EITLHPKEFNKGQIWGGLLFGLGWAITGACPGPLFATIGSGATVIVVSLLSAVAGTWV
                     YGKFRDRLPH"
     gene            6659..7717
                     /locus_tag="PKU82_04890"
     CDS             6659..7717
                     /locus_tag="PKU82_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82245.1"
                     /translation="MTVGITTGFSQNLRKTNNAVLRFDSSHDKPIQNLATEFISNNTS
                     KIQSTAPAFSFTESPYNHNMHITSDGTYYYTINGGSASNGQINKFDLNGVLLQTFSIG
                     IDGRGLSYNFTDGFLYASVYGGDIVKITDLVTGAFTTLFSGAMQNSQASFAISPDGTK
                     FYDFFQGTLRIHDFTTGSVINTLSGLLYGLGNYGGEAAVAVDSSHIYTWDAVTRNVYS
                     YNLTGTLIQTLPLDSGDNGISLSIANGYLFVSKDANYAIGTWYGYSISSSTSITELQN
                     NEPIIVYPNPTSGVINIDADIKTIEIFNVSGQKIFATTNINNLYQNKLDLSHLSKGIY
                     FAKLDGGDFLQTTKIIIQ"
     gene            7870..8541
                     /locus_tag="PKU82_04895"
     CDS             7870..8541
                     /locus_tag="PKU82_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOZ82246.1"
                     /translation="MISVSEIIIRLSLASFFGALIGLERERKHWAAGLRTHMLVCVGS
                     CLMMMVSAFGFSDILGTPDITLDPSRIAAQVVTGIGFIGAGAILFSKNETIRGLTTAA
                     GIWTVAGIGLATGGGMYLAAAVATAIAIIILWGLQPIEKMYFEKYKQKSLRITTSHDF
                     GDSEFLTALLKENGILLISLNFTRTNEYLIFQIQLEHADAGTIESVINILKKNSAIKE
                     IQWLN"
     gene            8704..9171
                     /locus_tag="PKU82_04900"
     CDS             8704..9171
                     /locus_tag="PKU82_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOZ82247.1"
                     /translation="MINIGINKKQAKQLAAKLNLLLADYMVFYQTTRGLHWNIRGEKF
                     FELHLKFEELYNNLLVKVDEIAERILTLDETPLHTFSDYSKASQLKEIKNVSDPSRSI
                     KAILDAFETILIKQREILRMSADADDEGTNALMSNYISEQEKLVWMYSAYYNK"
     gene            9173..9490
                     /gene="trxA"
                     /locus_tag="PKU82_04905"
     CDS             9173..9490
                     /gene="trxA"
                     /locus_tag="PKU82_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOZ82248.1"
                     /translation="METTTQNRTFNDIINGSKPVLVDFFADWCGPCKMMAPILHEVKN
                     EVGEKAVILKIDVDKNPAVAAAYEIQGVPTLILFKAGKVLWRQSGVVNKPQLLRIIEQ
                     NIG"
     gene            complement(9541..10431)
                     /locus_tag="PKU82_04910"
     CDS             complement(9541..10431)
                     /locus_tag="PKU82_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HOZ82249.1"
                     /translation="MLTIKKLFFIGFTFITLHATAQDDLMKMLNESGEKEKKEEVTAT
                     FKSTRLINGHSVENNKQGVLQLMILHRFGQINSGGYEFFGLDNATMRLGFDYGVTDWL
                     NVGIGRSTFEKMYDGLLKVKLLKQRKSGTPVTLTAVASMQLNSLKFTDPQRKNYFSSR
                     LTYSYQLLAASKLSNAFSVQLMPTLIHRNLVPTANEKNDVFALGGGGRIKISKSIAFT
                     AEYYYVFPDQISSTYKNSLAVGFDIETGGHVFQIHFTNSRSMVEKGFIAETTGDWLDG
                     GIHFGFNLNRVFTVYYPKEQ"
     gene            complement(10442..10993)
                     /locus_tag="PKU82_04915"
     CDS             complement(10442..10993)
                     /locus_tag="PKU82_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ82250.1"
                     /translation="MKKFILCVLMTGMYYTQAQNVFTTNAGTISFFSEAPLENIDATS
                     KKGHCILNVTTGDIVSLVAIRSFKFEKALMEEHFNEKYLESDKYKDAVYKGKIQEPLI
                     TDRDTSYQVTINGNITIHGIEKPANYKANYSFKGGVPAIEGSFNVALKDHNVEIPKLV
                     IQNIAEVVKVTCKFELAPYQKKD"
     gene            complement(11004..11375)
                     /locus_tag="PKU82_04920"
     CDS             complement(11004..11375)
                     /locus_tag="PKU82_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82251.1"
                     /translation="MNKSLILISGIFIALGISSCYYDVEDELYPNTGNCDTLNITYSQ
                     TVAPIISSNCKVCHSASMAQGNVVIDTYAAIKTYVDNGKLLGSLNHDSGYEPMPKNQN
                     KLSDCNIKKVSIWINQGAPNN"
     gene            complement(11382..12083)
                     /locus_tag="PKU82_04925"
     CDS             complement(11382..12083)
                     /locus_tag="PKU82_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82252.1"
                     /translation="MNRTVFFWLMLLTIATSSCKHEPQDLAIATNDSTATEDCSNDTI
                     YFKNIIQPIINASCAYSGCHDAATAADGVILDTYANIIRTGKVKAGKPEDSKLYKSLI
                     ENDPDDIMPPPPGSPLSSVQINDIYQWIMQGAKNNECLGCDSVNVTFSGSILPIMQTI
                     CQTCHSGIAPLGSLSLVSYDDVSAVAMTGQLVGAITHADGYFTMPDKEDSTKYIPNCK
                     IKQIIKWVNDGYPDN"
     gene            12240..12515
                     /locus_tag="PKU82_04930"
     CDS             12240..12515
                     /locus_tag="PKU82_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOZ82253.1"
                     /translation="MNGMLLFLDNLGGGELLVIMVFVLFFFGSKKVPDLARGLGRASR
                     EFKDAMNGVQREIEQSMQTPPPSQQPQKNVENPVTPEKNGGVDEKTD"
     gene            12634..14823
                     /locus_tag="PKU82_04935"
     CDS             12634..14823
                     /locus_tag="PKU82_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOZ82254.1"
                     /translation="MSNVRFRALDATNGRIPVKITPPSVKVSDYFATNVFTKEAMFKF
                     LPKNAYNAVISAIETGEKIDRKIAGEIAHGMKEWAISKGATHYTHWFQPLTGATAEKH
                     DSFFDMSNDNSFEHFSGSSLVQQEPDASSFPSGGIRNTFEARGYTAWDPGSPAFIIES
                     PNGRTLCIPTIYVSYTGETLDYKAPLLKALHTLNEVAVPVAQYFDKDITRVSTTLGIE
                     QEYFLVDTSLFYARPDLAITGRTVFGHSPAKGQQLEDHYFGSIPDRVNAFMHDFEIEA
                     YKLGIPLKTRHNEVAPCQFECAPIFEEINLAVDHNQLLMDLMDKVARRHHFKVLFHEK
                     PYAGINGSGKHNNWSMVTNTGKNLLSPGGNPKSNLMFLTFFINTIKAVHEHADLLRAS
                     IASAANDHRLGANEAPPAIMSVFLGAQLSSILDNIEKKVGSSKMSVEDKTALKLNIGK
                     IPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSNSMAILNTIVADQLRKFRKEVD
                     ALIKKNVKKDEAILRVLRQYIVETKSIRFEGNGYSEEWVKEAKKRGLSNHQTTPVALD
                     AMVSKKSLKLFEDNHIFTHREAEARHEIMLETYIKKIEIESRVIGDLAQNHIIPAAFR
                     YQNFLAETVDNLKDCDLKSETYATQLQLIKEISAHVNAIKKNVDTMLASRDKGNKIAD
                     IRDKAIFYCDKVKVSFDTIREHVDALEMIIDDEIWPMVKYRELVTIR"
     gene            complement(14938..15513)
                     /gene="nadD"
                     /locus_tag="PKU82_04940"
     CDS             complement(14938..15513)
                     /gene="nadD"
                     /locus_tag="PKU82_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOZ82255.1"
                     /translation="MKVGLFFGSFNPVHNGHMMIANYFAEFTDLEQVWLVVSPHNPLK
                     PAGSLLNDYQRLHLVETAIGSYKKIKISKIEFGLPKPSYTINTLVHLNEKFPQHEFVL
                     IMGSDNLITFHKWKNFEQILENYKIYIYPRPDSDGGELKNHPSVKIIQAPLIEISSTF
                     IRQAIKEGNDVRFYLPESVYNYIDEMNFYKK"
     gene            complement(15514..17022)
                     /locus_tag="PKU82_04945"
     CDS             complement(15514..17022)
                     /locus_tag="PKU82_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOZ82256.1"
                     /translation="MVCRILIAAFFLFLNSNIFAQTDSAVANGFKTFYYQNGNKSSEG
                     NFVNGKPEGLWKSYFENGKLKSEGNRHNNKPDGLWKFYNENGKITSELTYTKGLKNGL
                     QRYYFSDGKLKQEEEVKEGIRQGWVKDFGENGQVVNIIPFKNGVEDGVAKSYAEDGRL
                     ITITTYHEGYVRSEERINRIDKFGLKQGVWKVFFDNDKLKEETEYLDDKKNGFYRVYN
                     NDGTLIISERYKNGELIIDKKSTTKFEVKRDFYAGGKIKSIVNVINDKRQGVEKIYDE
                     KGNVTSGKLFEDDKPIAEGITDENGNRQGEWKFFYDDGSVHSIGKYKDGKRIDEWKFL
                     FQNGAVQQIGNYKNGLPEGEWKWFFKNGELRRDESFVNGKEEGISKEYNDSLQLIATG
                     KYVDGKKEGLWKYNNGEYIAEGEYADGLKFGTWHQTYKNGKTAFTGKYIDGLEDGEHI
                     YYNDNGKLHEVRQYKQGQRNGLWKIYDDKGVLILTINYQADKITRIDKIRIN"
     gene            complement(17012..18007)
                     /gene="nadA"
                     /locus_tag="PKU82_04950"
     CDS             complement(17012..18007)
                     /gene="nadA"
                     /locus_tag="PKU82_04950"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOZ82257.1"
                     /translation="MTEVSEIILKHGYLPESIPASMDLFEEIEKLKKEKNAVILAHYY
                     QESDIQDVADFVGDSLGLAQQAEKTNASMIVFAGVHFMAETAKLLNPDKKVVLPDLNA
                     GCSLASSCPPDDFKKFKEQHPGHVVISYINCTAEIKALSDVICTSSNVVKIVESFAPD
                     QKIIFAPDKNLGAYINKKIGRDMVLWDGSCIVHEIFSEQKIIQLKERYPEALVIAHPE
                     CEDHVLRHADFIGSTTGLLKYTKDNPAKAFIVATESGILYQMQKQSPEKMFIPAPPEN
                     NCACNDCPYMKLNTLEKVYLCMKNEVPEVIIPKHLQQAALQPLKRMLEISKQHGL"
     gene            18495..18926
                     /gene="rpiB"
                     /locus_tag="PKU82_04955"
     CDS             18495..18926
                     /gene="rpiB"
                     /locus_tag="PKU82_04955"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOZ82258.1"
                     /translation="MQSIIIGSDHAGFTLKEAIKKRFSSTFQITDKGTFSSDSVDYPD
                     YAHAVAGEVGNGKYTFGILLCGSANGVAITANRHSGVRAAICWMPEIADLARKHNNAN
                     IICIPARFVSDETAFLMIEKFFSTEFEGGRHQKRIDKINCV"
     gene            18945..19664
                     /locus_tag="PKU82_04960"
     CDS             18945..19664
                     /locus_tag="PKU82_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOZ82259.1"
                     /translation="MKFKFILLICVLPFFVNAQDEQDTSAVTSKKQKREIPRFAIKVT
                     GGLSWRLADVSPDENDLTKAYIKDLKSGNGFEISAKYFFNSKFGVGLKYNSHLSKVRY
                     DNMYFSYNNQNYFGTVSDDISINTIAVTFSGRIYNRQKTGYFTFDGGLGLMTYKDDTK
                     IATNKVNVKGNTLGFNISAGVGVNITRNLCLVIGFNFINGTLSQYDETENGVKKRVTL
                     NESEYQSLSHIDIFEGIVFTF"
BASE COUNT         6479 a         3336 c         3418 g         6515 t
ORIGIN      
        1 tatcgtttgt gcatagtttt attttcagca aacgtaacag atttttttac ttaatgcaaa
       61 aaatgatgaa tcatacttta atttaactgt taatgataga gctaaaacac atgaagaaat
      121 taataccgct cttgactccc gggtatttgg gctgccttta acgcctaata cataaaatgg
      181 ttacaacaat cggcatatta atttacaata cagaatgtaa tgtaacctaa tagccttttt
      241 ccttgtatag aggttataaa attcagtaaa gatgacaaca aaatcatttt ggtcacaacg
      301 tgccatgctt agtatcaggt caaacaaacg attgtttctt ggctgccttt tggcaggtag
      361 tgttggcgct tcagcctatt tgtttttaat gcctaaggtt tatgaatcgc aggctatctt
      421 aaaaaatatt aaccccgata aacagtcaac tgccattgct gcagaaatga ttcgttcacc
      481 caaaacaatt caacagtctg taaaaggaat ggggctgaac gtagaatatt ttgtaactaa
      541 aaactttcag acatcagaat gttttgacaa taagcctgta aatatttcat ataaattaaa
      601 agatgagtcg ttcagtcagc aaagttttag tctgcaacgc gatggtgaaa aaacctacat
      661 acttgaatat gatgatcagg cagggcatca acaaaaacga ggcagctata atgaattgct
      721 cgaccttgga tcgcttgctg taaccattga aaaaacgcct tatctgaatt tgcttgatga
      781 taaagatgaa gtttcattta ccatttattc tgacagccgc ttagctaaca aaattgctga
      841 gcaaaatctg agttgttcta ttgaagatgg tggtgttata aaactaaaca gccaaagcgc
      901 taaccctgct aaggcatatc gtatagcaag tgctgtctct aaaggttatg gcaatgccgg
      961 acaacaggca aacgaatcac attccttagc caatgtgcag ctaatcaata atcaacttga
     1021 ccgtgtttcg caggagttgg ataatgcaca acgcgagttg tcacactttc aaagtgtaaa
     1081 caatgcctat gaaatgccac agcaggcggg agcagtatta aacactgtta gtcaactaca
     1141 gattcagaag gtagaagcag atatgcaatt agctgcctta gataatctga gtgattatat
     1201 gcgcaagaac agaaatgtga acaccatttc accggagtat ggaaccatta cagatattat
     1261 ttatacagaa acatatctga agctgaatga taaagttgcc gagcgtcagc aactgttaga
     1321 gcaaggtgca gacctttcaa aagttgataa ggaaataagc tcgcttaaag aaatgcttgc
     1381 ggagagtatt cgcaatacaa gaaaaaaact gaccatcaga caagaaaccc ttgcagcagc
     1441 tattgtttct gctaaagaca gaattcaaaa tttacctcag acagaagaaa aactacagca
     1501 actaaacaga agcatttatt tatatactaa gctttatgat tttctgattc agaaaagagc
     1561 tgaggctatg gtagaggctc ctgtattgcc accatcatca tacattttaa atgaggcaaa
     1621 aatgcctaca tcacctatca gtccaaatgc agcaaaagtt tggtcgctgg cattgcttgc
     1681 atcactattg tttggtgctt tagttactgc tgttaagccg ctattgaaaa taaaaatcag
     1741 caatagaaaa gacctgcaaa aatttacaga cattcctttt atcgccaata ttgaagccgg
     1801 tggtaaaaat catgatttca gtgagtcgtt tatgacactc tgtactaaaa tactcatgct
     1861 tggaaataaa cagcctgtgc agatgataac tataacatca gcaaaagccg gtgaaggaaa
     1921 aactactgtt gccgaaaatt tagctaaggc tatgggcaag ctgggtaaaa aagcattgtt
     1981 aattgatatg aatcctataa atccttcatt aaaaaacaca cttggtaatg aggaagaaaa
     2041 cagttttgct caggttattg acgatgataa aaatctacat caggcagtac aaataaccaa
     2101 ttatgaaaat gttgatctgc taagtgccgg atatcttgcc catggcgtaa acacacttct
     2161 tataaacgat aaaactggca acattcttgc agatatgaaa acgttatacg attacgtgat
     2221 atttgatacc cccgaaacca gtaattatat ggatgctgtt ccgttgatga aaatgagtga
     2281 tttaaacatg tttgttatta aagcaaacag tgccacattc gatcagcttg agtatgcatc
     2341 gcaaatgaaa aaagattttc aggttgagaa cctatttatg attattaatg gcatgacgga
     2401 aaccatgaac cactccggca tgcaaacttc gggtaaggta aaacaaattc gcagacgtga
     2461 acaagcagag gcagaaatca ggtttatgcc aagaatgatg aaaaaagcag cattatggtt
     2521 ttattgattt aatttttatg ggggataaag caaaattctt aaatcacttt tttgagcgta
     2581 tcagcgacat cagatatgcc gcaatgggat taactgtttc gcatcccgaa gagattgctg
     2641 aagatattat tcagattgca attaaccggg aagatacacg caaaattctt acggtgcttg
     2701 aattttcgcc ttatttaaaa agagtgcgtt tagaagaaaa acccttttac aatttcattc
     2761 atctgaaatt gagtaatggc caatggatac aaattcaact aatctatggc tttgtaagaa
     2821 aaggcattca gtttatgaat gaaaaagagg tgttgaacga gtgtaccatt aacagtggag
     2881 gaataaaagt taccagccag catctgaatt ttgaatatgt gtggctgata cattgtctga
     2941 acaaaacaaa tgttcccaat catcagcatt tatttttctc gcaatataac cgaaatgaac
     3001 gctctgccat ttttgcacac atacgaagca gatatttttt agaactcaat gtattggacg
     3061 aactatatac ttttcacaaa aaaacctatc gtcaggttta tgaaaaaata attcgcagaa
     3121 aagaaaatac atgggcaaaa agaaacattc gtaaaacact ttatgccttt tacagtgcat
     3181 taaaccttat aaagacaaaa agtattaaag tgcagtttaa gggttcggga tatcatccgt
     3241 tttcaaaaac agaaagagca agagtttatt agtcttttct gttttgttta acccattggt
     3301 taatgtactc aacaatttca ttggtgtttg tacccggagc aaacagtttg cccacaccca
     3361 tatcatttag ttttttcata tcggcatcag gaataatgcc tccccctgta acaagtacat
     3421 cggtcattcc tttttctttg agcaactcca taattttggg aaatacagtc aggtgtgcgc
     3481 cagagagaat tgaaacacca ataacatcaa catcttcttg caaagctgtg ttaacaacca
     3541 tttgtggtgt ttgacgaagg ccggtgtaaa taacttccat gcctgcatca cgaagaaaag
     3601 aagctataac ttttgcacca cgatcgtgac catcgaggcc tactttggaa attaatactc
     3661 tgattggtct gttcataagt gcaaaaataa caaaggtgtg ttatctttaa agaacattta
     3721 tcagtattct tgaatgtgat atgttcataa attgatatta tcgtggattt ctaaataaac
     3781 agtaatttta ttttagcatc catgaaaaaa ttagttgttc aattattttt acttttctcc
     3841 tacggaacta cagttgcaca ggatacgaac aataatattc caaaaccgca aagtattatt
     3901 ccgcagtccg gcatctttat tttgcatcgt aaaatagcaa tagtagaagg tcctgcatgg
     3961 gtaacgggtg atgcagcagc gagggtttat tgcacacaat taaaagatag tataggttta
     4021 caccttgcca ttgtgaatgc tatgcgcgaa ggaacatttg aaaatgcaat taatctgaaa
     4081 aaaataaatg atgcagcatt aggaaatgcc ggttacagaa ttaaaattaa aagacctgcc
     4141 attattgtag aggctaataa tgatacggga ctgacgaatg cacttcaata tttatttcaa
     4201 ataataccta agaaattctt tgaggacaaa aatccaaaag caaaaattcc actcagctgt
     4261 agtgagatta catgtaagtt ttgaaagtac gaaggttaga aagttaaaaa ttttgataaa
     4321 tatttaaagc accaagaata agcatctaac tctattttgt aatgacaaaa tattgaatta
     4381 accgatattt ttagattgtt gtttaagtaa gaaggagatt tttttaatac ataacatgaa
     4441 aatatttatt gtatattaaa aaacactatt taaattaatg taactgaagt tgcattacag
     4501 ttatgatttt tatcagacct ttgccgtcac aattaaaatt ctaaaataag gtggtggaaa
     4561 tttttggata tgcatgtgca atacttatgg gattgtcgtt gggattaatt ggtggtggag
     4621 gcagcatttt aacagtgcct atattggttt atttattttc tatagatgtg gttacagcaa
     4681 cagcctactc gctttttatt gttggcttta caagtttgat aggatcgttt aatcatatca
     4741 gaatgggaaa tgttcatctg cgaaccgcag tgctatttgg tattccatca attattgctg
     4801 tattcattac aagagcattt attgtacctg caatacctca gcatttattc aacattggaa
     4861 cactacagat aacaaaaccc attgcactac tgttgttgtt tgctgttatc atgttgctgg
     4921 catcattctc tatgattcgc aagaaaaaag aaaaacccga ttatgataaa gtgcaataca
     4981 attatccatt aattataatt gaagggtttg ttgttggcac aattacaggg ttagtaggtg
     5041 caggtggagg atttttaata gtgcctgcat tagtattgtt ggcgcatttg cccatgaaaa
     5101 aagcaattgg atcatcgtta ttgattattg caaccaactc tttattgggt tttacggtta
     5161 gccttttaca tagtgagaaa acagactgga aatttctgtt aactttttct gctattgcta
     5221 tcataggaat tattaccggc agtatgtttt cgaaaaatgt ttctaacgaa aaattaaaac
     5281 ctgcatttgg atggtttgta ttaataatgg gtgtttatat tattcttaaa gaaactttat
     5341 tgaaataatt aaaccctttc atttcaattt gtgttttcat ttcatttctc tttacaataa
     5401 actttatttt aaatattatt acttaacact aaatttattt taaactatgc ttgaattaat
     5461 tcttaaacca tggccttggt atgttgcagg ccctctgata ggactcattg tgcctgcatt
     5521 acttatactt ggaaacaaat catttggaat cagttcttca ttccgtcatg tatgtgctgc
     5581 ctgtatgcca tcaaacattc cattctttaa atatgattgg aaaaaagaaa ggtggaattt
     5641 attttttgtt gccggaataa tggctggtgg attcttagct gtgaatttac tctctgcatc
     5701 tactaaagtt gatatcaatc cttcattagc agcatctcta aatgaaaaag gtataaccga
     5761 ttttagtggt ttggttccgg cagatttatt taactggaat agtctgttca cattaagagg
     5821 ttttattatg atggttgtag gaggattttt agttggcttt ggaacccgat atgcgggagg
     5881 ctgtaccagc ggacatgcta ttatgggatt atcaacgctg caattgccct cgctcattgc
     5941 cacctgttgt tttatggccg gtggttttat aatggctaat ttaattttac cttatatact
     6001 ttcactctga aattaatatt atgaaaacag aagttacttt agaaaaagaa caaaacacag
     6061 attttgaaac acgttcatta gatacaattt gtgtaaacga aagtcatctt gaacatagtt
     6121 gggagcataa cttaaaatac agccttatgg gaattctgtt tggaattatt ttagtgaagg
     6181 cagaagtggt atcgtggttc agaatacagg aaatgttcag gcttcaatca ttccacatgt
     6241 atggtatcat tggaagtgct gttgttgttg gagctttgtc tgtttggctt attcgtaaat
     6301 tcaacattaa aacaatttat ggagaagaaa taaccctaca tccaaaagaa tttaataaag
     6361 ggcaaatttg gggtgggcta cttttcggat tgggatgggc tattacaggt gcatgccccg
     6421 gaccactgtt tgctacaatt ggctctggtg ctactgtaat tgttgtgagt ttattaagtg
     6481 ccgttgccgg aacatgggtg tatggtaaat tccgagatag acttcctcat tgattaaaag
     6541 tttgttgaat ccaattgtat tatttaattt agtaggataa aattattctt ataaatttat
     6601 tattgataaa cttttaatct ttataaatat gaaaaaatta ttttacattt tattattcat
     6661 gactgttgga ataacaactg gattttcaca aaatctgaga aaaactaata atgcagtttt
     6721 aaggtttgac tcatcgcatg ataaaccaat acaaaacttg gccacagaat ttatttcgaa
     6781 caatacttct aaaatacaat caactgctcc ggcattttca tttactgaaa gtccatataa
     6841 tcataatatg catattacct ctgatggtac atattattat accataaatg gtggtagtgc
     6901 ttcaaatggt cagataaata aatttgattt aaacggtgtt ttacttcaaa ccttttcaat
     6961 tggcattgat ggcagaggtc tgtcatataa ttttactgat ggctttttat atgcatcggt
     7021 ttatggagga gatatagtta aaattacaga tttagttacc ggagcattta caactttgtt
     7081 ttcaggtgca atgcaaaata gtcaagcctc atttgctata tctcctgatg gaacaaaatt
     7141 ttatgacttc tttcagggaa cattacgtat tcatgatttc actacaggtt ctgtaataaa
     7201 caccttgtca ggtcttttat atggtttagg aaattatggt ggcgaagctg cggttgctgt
     7261 tgattcaagt catatttata cttgggatgc tgtaacaaga aatgtatatt cctataatct
     7321 caccggaaca cttatacaaa cattaccgct tgattcaggt gacaatggaa tatctttaag
     7381 tattgcaaat ggatatttat ttgtttcaaa agatgctaat tatgccattg gcacctggta
     7441 tggatatagc atttcatcat ctacatcaat aaccgaactt caaaataatg aacctataat
     7501 tgtatatcca aatccaacat caggagtcat aaatattgat gcggacataa aaactattga
     7561 aatatttaat gtttctgggc aaaaaatatt tgccactaca aatattaata atttatatca
     7621 aaacaaactc gatttatctc atttatccaa aggaatatat tttgcaaaac ttgatggtgg
     7681 cgattttctt caaacaacaa aaattatcat tcagtaattc tatttctcta atatgttttt
     7741 actttctata aaaagtttag tcctgatttt agatttattt tagttgttga aacaaatcta
     7801 tttttggtct gttaaacata ggttgtttaa acatttattt atgtaataac tgtagtttat
     7861 ttccttaata tgataagtgt ttcagaaatc attatacggc tttcattagc atcgtttttt
     7921 ggtgcgctta taggattaga acgagagcgg aagcattggg ctgccggttt gcgtacacac
     7981 atgcttgttt gtgtaggttc ctgcctgatg atgatggtat ctgcatttgg attttcagac
     8041 atcttaggaa ctcctgatat aacacttgac ccttcacgta ttgcagcaca agtagtcacc
     8101 ggtataggtt ttataggtgc gggtgcaatt ctattttcga aaaatgaaac tattagagga
     8161 ttaactaccg ctgcaggaat atggactgtg gcaggaattg gcttggcaac aggaggaggc
     8221 atgtatctag ccgctgctgt agcaacggct attgctataa ttattttatg gggacttcag
     8281 cctatagaga aaatgtattt tgaaaaatat aaacaaaaaa gtttgcgtat aactacaagt
     8341 catgattttg gtgattcaga atttctgaca gcacttttaa aagaaaatgg catattatta
     8401 atttcattaa attttacaag aacaaacgaa tacttgattt ttcaaattca gctcgaacat
     8461 gctgatgctg gaacgataga atcagttatt aatattctca aaaaaaattc agccattaaa
     8521 gaaattcaat ggcttaacta agaattgaaa actctttcaa ttctaaaaat taagataaaa
     8581 acaaaagttc cgtttccttg tttaggactg tcaatacttc attagaagtg atgatttaag
     8641 tcagctattg atttatgatt taattttaat ttagcatcta aagatttaca aaagttctta
     8701 attatgatta acataggaat aaataaaaaa caggcaaaac aacttgctgc aaaacttaat
     8761 cttctgcttg cagattacat ggtattttat cagacaaccc gcggattgca ttggaatata
     8821 agaggtgaga aattttttga attgcatctg aagtttgaag aactgtacaa taacctgtta
     8881 gttaaggttg atgaaattgc agagcgtatt ttaactttag atgaaacacc gctgcatacc
     8941 tttagcgatt acagtaaagc atcgcaactg aaagaaatca aaaatgtatc agacccttca
     9001 agatcaataa aagcaatttt agatgctttc gaaactattt taatcaaaca gcgcgagatt
     9061 ttaagaatgt cggcagatgc agatgacgaa ggcaccaatg cattgatgag taattatatc
     9121 agcgagcagg aaaaattagt ttggatgtat agtgcatatt acaataaata aaatggaaac
     9181 aacaacacaa aacagaacat ttaatgacat catcaatggc agtaaaccgg tattggttga
     9241 tttttttgcc gactggtgcg gaccttgcaa aatgatggca cccatactgc atgaagtaaa
     9301 aaatgaagta ggcgaaaaag cagtaatact taaaattgat gtggataaaa atccagctgt
     9361 tgcagcagct tatgagatac agggagtacc aacattaatc ttgtttaaag ccggaaaagt
     9421 gttgtggcgg cagagcggtg ttgttaacaa accacaacta ctgcgaatca ttgaacaaaa
     9481 tatagggtag gttttatatg gaatgagcct acaaagctaa ttccataaac acaaggaata
     9541 ttactgctcc ttagggtaat aaactgtaaa aaccctatta aggttaaacc caaaatgaat
     9601 accgccatca agccagtcgc cagtagtttc ggcaataaat cctttttcta ccatactgcg
     9661 tgagttggta aaatgaattt gaaaaacgtg acctccggtt tcaatatcaa agcctacagc
     9721 aagtgagttt ttatatgtgc tgctaatctg atcaggaaat acataataat actctgcagt
     9781 aaatgctata ctcttggaaa tttttattct tccgccaccg cctaatgcaa atacatcatt
     9841 tttttcatta gctgtgggta ctaaattgcg gtgaatgagt gttggcatta gctgaacaga
     9901 aaacgcatta gaaagtttag atgctgccaa caactgatag gaataggtta gcctggaaga
     9961 aaaataattt ttacgttgag ggtctgtaaa ttttaatgag tttagttgca tggatgcaac
    10021 ggcagtaagt gtaaccggtg ttccactttt acgttgcttc agtaatttaa cttttaataa
    10081 tccatcatac attttttcaa atgtgctgcg ccctattcca acgtttaacc agtcggtaac
    10141 accatagtcg aaacctaaac gcatggtagc attatctaaa ccaaaaaact cataacctcc
    10201 actatttatc tgcccgaaac gatgcaagat cattaactgc aatacacctt gcttattatt
    10261 ttcaacactg tgcccgttaa tgagtctggt tgatttaaat gttgccgtta cttcttcttt
    10321 cttttctttc tctccacttt cattgagcat tttcatcagg tcgtcctgag ctgttgcatg
    10381 cagtgtgata aaagtaaatc caataaaaaa cagtttttta atcgttagca taaattcttt
    10441 tttaatcctt cttctgatat ggagcaagtt caaatttaca agttacctta acaacttcgg
    10501 caatattctg aataactaat ttaggaattt caacattgtg gtcttttaag gcaacattaa
    10561 agctaccttc aattgcagga actcctcctt taaaactgta gtttgcctta taatttgccg
    10621 gtttctcaat accatgaata gtaatatttc catttattgt tacctgatag gaagtgtctc
    10681 tatccgtaat caatggctcc tgaattttac ctttatatac agcatctttg tatttatcac
    10741 tttcaagata tttttcattg aagtgttctt ccatcaacgc tttttcgaac ttaaaagaac
    10801 ggatagctac taacgaaaca atatcgcctg ttgtaacatt caaaatgcaa tgcccttttt
    10861 tgcttgtagc atcaatgttt tcgagcggtg cttctgaaaa gaaagaaatt gtacctgcat
    10921 tggttgtaaa cacattttgt gcttgtgtat aatacatgcc tgtcattaat acacataaaa
    10981 taaatttttt cattttttaa tatctaatta ttgggtgctc cctgattaat ccagattgaa
    11041 acttttttaa tgttgcaatc acttaattta ttttgatttt ttggcatagg ttcatatccc
    11101 gaatcatgat tgagtgaccc taatagtttt ccattatcaa cataggtctt aattgctgca
    11161 taagtatcaa taacaacgtt gccctgcgcc atagatgccg aatgacaaac tttacaattg
    11221 ctgcttataa ttggtgccac tgtttgtgaa taggtaatgt taagtgtatc acagttgcct
    11281 gtatttggat ataactcatc ttctacatca taataacaac tactaatgcc gagtgcaata
    11341 aaaatacctg aaataagaat taaactttta ttcatgcttt gttaattgtc ggggtaacca
    11401 tcgttaaccc atttaatgat ttgttttatt ttacagttag gaatatattt tgttgaatct
    11461 tccttatcag gcatggtaaa atatccatct gcatgagtta tagcgccaac caactgtcct
    11521 gtcattgcaa cagcagatac atcatcataa gaaaccaatg aaagacttcc caatggtgca
    11581 attccactat ggcaggtctg acaaatggtt tgcataatgg gtaagatact acctgaaaat
    11641 gttacattaa cactatcaca acccaaacat tcattgtttt tagcaccttg cattatccat
    11701 tggtaaatat catttatctg tacgctgctc aatggtgaac ccggtggagg tggcatgata
    11761 tcatcaggat cattttcaat aagtgatttg tagagtttgc tgtcttcggg tttaccggct
    11821 ttcacttttc ctgttctgat aatgttggca taagtatcta aaataactcc atctgctgca
    11881 gttgcagcat catgacatcc gctataggcg catgaggcat ttattatagg ctgaatgatg
    11941 tttttaaaat aaatggtatc gttgctgcaa tcttcagtgg cagtgctgtc attggttgca
    12001 atggctaagt cctgtggttc gtgcttacag gaagatgttg caattgtcaa tagcatcaac
    12061 caaaaaaata ctgttctatt catgcatgaa ttaatttaga aacaaataaa aaagatatat
    12121 tctaattaaa gtctataaaa atgtattaat gggaaaattt atcggctgaa aggtaataaa
    12181 cacaaatatt cattgtttca acatgggcat ttgagttact tttgctcaaa ttttaagata
    12241 tgaacggcat gcttttgttt ttagataatc ttggtggagg cgaattgttg gtaatcatgg
    12301 tgtttgtatt attcttcttc ggatcaaaga aagttcctga cttagccaga ggcttgggcc
    12361 gtgccagccg cgagtttaaa gatgctatga atggtgtgca gcgcgaaatt gagcagagta
    12421 tgcaaactcc gccaccatca caacaaccac aaaaaaatgt tgaaaatcct gttacccccg
    12481 aaaaaaatgg gggggtagat gaaaaaactg actaattttt ggtgaaacac tctatttttt
    12541 aggggggtgt ttgaaaaaaa acaatatttt tcttgacatt gccctttttt tcaatgtatt
    12601 tttgagccgt tttaaaaaaa atccaatcac tatatgagca atgttcgttt ccgtgccctc
    12661 gatgctacta atgggcgcat acctgtaaaa attacccctc cgtcagtaaa ggtatctgac
    12721 tattttgcca ccaatgtatt caccaaggaa gctatgttta agtttttacc taagaatgca
    12781 tacaatgctg ttatctctgc aatagaaacg ggtgagaaaa tagaccgtaa aatagctggt
    12841 gaaattgccc atggcatgaa agaatgggct atcagcaaag gtgctacaca ttacacgcat
    12901 tggtttcaac cactcactgg tgctactgcc gaaaaacacg acagcttttt cgacatgagt
    12961 aatgataact ctttcgaaca tttttcagga agttctttag tacaacagga gcctgatgca
    13021 agcagttttc caagtggagg aataagaaat acttttgaag cacgtggcta cactgcatgg
    13081 gatccgggct ctccggcatt tattattgaa agcccaaacg gacgcacact ttgtattcct
    13141 acaatttatg tttcttacac aggtgaaaca ctcgactata aagcgccatt gttaaaagca
    13201 ttgcatacac tgaacgaagt agcagttccg gtagcacaat attttgataa agacattaca
    13261 cgtgttagta caacattagg tattgaacaa gaatattttt tagttgatac ttctttgttt
    13321 tatgcacgac ccgatttggc aattacagga agaacagttt tcggtcactc acctgcaaaa
    13381 ggacaacaat tagaagatca ttatttcggt tctattcctg atagagtaaa tgcctttatg
    13441 cacgattttg agattgaagc atataaactt ggtattccat taaagacccg tcataatgaa
    13501 gtagcaccat gtcagtttga atgtgctcct atttttgaag aaataaatct tgctgttgat
    13561 cacaatcaac ttcttatgga tttgatggat aaggttgccc gcagacatca ttttaaagtg
    13621 ttatttcatg aaaaacctta tgcaggaata aacggtagcg gtaaacacaa caactggagt
    13681 atggttacca acaccggaaa aaatttattg tcgccaggag gaaatccaaa atccaacctc
    13741 atgtttttaa ctttttttat caataccata aaagctgttc acgaacatgc agacctgttg
    13801 cgtgcaagca ttgcttcggc agctaacgac catcgtttag gtgctaatga agcaccaccg
    13861 gccatcatga gtgttttcct tggtgcacag ttaagcagca tcttagataa cattgagaaa
    13921 aaagttggct ccagcaaaat gagtgtcgaa gataagacag cattaaaact caacataggt
    13981 aaaatacctg aaattttact tgacaataca gaccgcaacc gcacatctcc ctttgccttt
    14041 acaggaaata aatttgagtt tcgtgcagta ggttcttctg ccaattgcag caattcaatg
    14101 gctattttaa atactattgt tgccgatcaa cttcgcaaat tcagaaaaga agtagatgct
    14161 ttaattaaaa agaatgtaaa gaaagacgaa gctattttgc gtgtgctgcg tcagtatatt
    14221 gttgaaacaa aaagtattcg ttttgaaggc aatggctata gcgaagagtg ggtaaaagaa
    14281 gctaaaaaga gaggactttc caatcatcag acaacacctg ttgcattaga tgcaatggtt
    14341 tcaaaaaaat ctctgaaact ttttgaagac aatcacattt ttacgcatcg tgaagccgaa
    14401 gcgcgacatg aaattatgct tgaaacatat atcaagaaaa tagaaattga aagtcgtgtc
    14461 attggtgacc ttgcacaaaa tcatattatc cctgcagcat tccgatatca gaatttttta
    14521 gcagagactg ttgacaacct caaagactgc gatctgaaat cagaaactta tgctacacag
    14581 ctgcagctga taaaagaaat cagtgcacat gtaaatgcca ttaagaaaaa tgttgatacc
    14641 atgcttgcat cgcgcgataa aggaaataaa atagcagata ttcgcgataa agcaattttt
    14701 tattgcgata aggttaaagt ttcctttgac acaatccgcg aacatgttga tgcattggaa
    14761 atgattattg atgatgaaat atggccaatg gttaaatacc gagaattggt tacgattaga
    14821 taattgattt gaacaacaaa aaaagctgtc tattatggca gctttttttg ttttgaatat
    14881 taatcaaaca cctatctgtt tttcaaaaac ttttaagctt ttaagctttc aaactcatta
    14941 cttcttataa aaattcattt catcaatgta attatacacc gattcaggaa gataaaaacg
    15001 aacgtcattt ccttctttga tggcttgtct gataaatgta gatgatattt caattaatgg
    15061 tgcctgaata atttttacag aaggatgatt ttttaattca ccaccatcag aatccggtcg
    15121 aggataaata tatattttat agttttctaa tatctgctcg aaatttttcc atttatgaaa
    15181 agtgataagg ttatcactac ccattatcag cacaaactca tgttgtggaa atttttcatt
    15241 cagatgaact aatgtattaa ttgtatagga tggtttgggt aatccgaatt ctattttcga
    15301 aatttttatt ttcttatagc tgccaattgc tgtttcaact aagtgtaatc tttggtaatc
    15361 gttaagtaat gatccggcag gtttaagcgg gttgtgtgga gaaacaacca accacacctg
    15421 ttctaaatct gtaaactcag caaaataatt ggcaatcatc atgtgtccgt tatgcacagg
    15481 attaaatgaa ccaaaaaata aaccaacctt catttagtta attcttattt tgtcaattcg
    15541 ggtaattttg tctgcctgat aattgatggt taaaatcaat acacctttat cgtcataaat
    15601 tttccacaaa ccattccgct ggccttgttt atactgtcgc acttcatgca actttccatt
    15661 gtcgttgtaa taaatatgtt cgccatcttc aaggccatca atatattttc ctgtaaatgc
    15721 agttttaccg ttcttatagg tctgatgcca tgtaccaaat tttaatccat cggcatattc
    15781 gccttctgca atatattcgc cattattata tttccatagc ccttcttttt tgccgtcaac
    15841 atatttacct gttgctatta gttgtaatga gtcgttatat tctttagaaa taccttcttc
    15901 tttaccattt acaaaagatt catcacgcct taattcacca ttcttaaaaa accatttcca
    15961 ctcaccttcc ggaagtccgt ttttataatt tccaatttgt tgaactgctc cattctgaaa
    16021 taagaatttc cactcatcaa ttcgtttacc atctttatat tttcctatag aatgcaccga
    16081 gccatcatca tagaaaaatt tccattctcc ctgtctgttt ccattttcat cggttattcc
    16141 ttcagcaatt ggtttatcgt cttcaaataa ttttcctgat gtaacatttc ccttttcatc
    16201 ataaattttt tctacaccct ggcgtttatc gttaattaca tttacaatag actttatttt
    16261 tccacctgca taaaaatcac gctttacttc aaatttagtt gtgctttttt tatcaattat
    16321 caactcgcca tttttatacc tttccgatat aattaatgtg ccatcattat tataaactct
    16381 ataaaaacca ttctttttat cgtcaagata ttctgtttct tcttttaatt tatcgttatc
    16441 aaaaaaaact ttccatacac cttgctttaa tccaaattta tctattctgt taattcgttc
    16501 ttcgctgcgc acataacctt catgataagt tgtgatggta attaatctgc catcctctgc
    16561 ataagactta gcaactccat cttcaacacc atttttaaat ggtataatat ttacaacctg
    16621 accattttct ccaaagtctt ttacccaacc ttgtctgata ccttctttta cttcttcttc
    16681 ttgttttaat tttccatcag aaaaatagta acgctgtagt ccgtttttta atcctttggt
    16741 atatgttaat tcactggtaa tttttccgtt ttcattgtaa aatttccata aaccatcagg
    16801 cttattgtta tgtctgttgc cttcggattt taatttacca ttttcaaaat acgatttcca
    16861 taaaccttcg ggcttgccat ttacgaagtt accttcgcta ctcttattac cgttctgata
    16921 ataaaatgtt ttaaagccat tggcaacggc actgtctgtt tgtgcaaata tgtttgaatt
    16981 aagaaataaa aaaaatgctg caatcagtat tctacaaacc atgttgtttt gatatttcaa
    17041 gcatgcgttt taaaggttgt agtgctgcct gctgcaaatg tttagggata attacttcag
    17101 gtacttcgtt tttcatacag agatacactt tctctaaagt atttaatttc atatacggac
    17161 aatcgttaca tgcacagtta ttttccggtg gtgctggaat aaacattttt tcaggtgact
    17221 gtttttgcat ctgatataaa attcctgact ctgtagctac aataaatgct ttagcaggat
    17281 tatcttttgt atatttcaaa agtcctgttg tgcttccaat aaaatcagca tgacgtaaaa
    17341 catggtcttc acattcagga tgtgcaatta ctaacgcttc gggataacgt tcttttaact
    17401 gaataatttt ttgttctgaa aatatctcat gtactataca actcccatcc cataaaacca
    17461 tatcacgacc aattttttta ttgatgtagg ctccaagatt tttgtcaggt gcaaaaatta
    17521 ttttttgatc aggtgcaaaa ctttctacaa tttttaccac attgcttgat gtgcaaatta
    17581 catcacttaa agctttaatt tctgctgtgc agttgatgta tgaaattaca acatgaccgg
    17641 gatgttgctc tttaaacttt ttaaaatcat caggcggaca actactcgct aatgagcaac
    17701 ctgcatttaa atcgggtaaa acaacttttt tatcggggtt cagtaatttt gctgtctctg
    17761 ccatgaaatg cacaccggca aaaactatca ttgaagcatt agttttttct gcttgctgtg
    17821 ccaagcccaa actatcacca acaaaatcgg ccacatcctg tatgtcggac tcctgatagt
    17881 aatgcgccag aataacggca tttttttctt tttttaattt ttctatttct tcaaacaaat
    17941 ccatggaggc aggaatactt tccggcaaat atccatgttt caaaataatt tcagacactt
    18001 cagtcatttt aaacaattat tattattaaa tatatataat taatttttta aaatattatg
    18061 ttgttataat tagtcggtta atatgttgta taactttttt aaaatgttat tgcattttaa
    18121 gttactgtac atgtactaac agtttataaa ctttttatta taactaactt tatgattaat
    18181 aaaggcataa atataactat taacaacggt taataagcca atatgtttat aaaaaacggt
    18241 taattaaatg tttattatgg tgaatggcta tgtttatata ttgactaaaa cttcgtgcta
    18301 aattattttg aattaagttt aaatctgata aggcatttta gaaataaaca ttgcaaacaa
    18361 aagaaatgat aatctatgaa cagaaaatat aaatatgaac aggaatgttg ttaattttac
    18421 accattataa aatactatta atcagcacta aaatatttga ctgttaatag cagttaataa
    18481 cttaaattta tcaaatgcaa agtataatta taggtagcga tcatgccggt ttcacattaa
    18541 aagaagcaat aaagaaaagg ttttcatcaa cttttcaaat tacagataaa ggcacattca
    18601 gttcagattc tgttgattat cctgactatg cacatgcagt tgccggagag gttggtaacg
    18661 gaaaatatac atttggaatt cttttatgtg ggagtgcaaa tggtgtggct attacagcta
    18721 acagacatag tggtgtaaga gctgcaattt gctggatgcc tgaaattgcc gatttagcca
    18781 gaaagcataa taatgccaat attatttgta ttccggcacg ttttgtcagc gatgaaacag
    18841 cttttttaat gattgaaaag ttttttagta ctgagtttga aggaggtcgg catcagaaaa
    18901 gaatagataa aataaactgc gtttaaaata gaaaaaagat agctatgaaa tttaaattta
    18961 tactgctgat atgtgtactt ccgttttttg tgaacgcaca ggacgaacag gatacatcag
    19021 cagtaacctc taaaaaacaa aaacgtgaaa ttccacgatt tgcaataaaa gtaacaggag
    19081 gtcttagttg gagattagct gatgtaagcc ctgacgagaa tgatttaaca aaagcctata
    19141 ttaaagattt gaaaagcgga aatggctttg aaatttctgc aaaatatttt tttaattcaa
    19201 aattcggagt tggcctaaaa tacaacagtc atctttcaaa ggtcagatat gataacatgt
    19261 atttctccta taacaatcaa aattattttg gcacggtgtc tgatgatatt tcaattaata
    19321 caattgcagt aacttttagc ggaagaattt ataacagaca aaaaacaggt tactttacct
    19381 ttgatggtgg acttggtttg atgacttata aagatgatac gaaaatagct accaataaag
    19441 ttaatgtcaa aggaaacaca ttaggcttta atatttctgc cggtgtgggt gtcaatatta
    19501 caagaaattt atgtttagta ataggtttta attttattaa tggaacctta tcacaatatg
    19561 atgaaacaga aaatggagta aaaaaacgtg ttactttaaa tgaaagtgaa tatcaaagtc
    19621 ttagccatat tgatattttt gaaggaattg tatttacctt ttaaatctat aaatcttttt
    19681 ttctgaattt acgcacagca atcatagttg gtacaataat ccatattaaa agtattagca
    19741 gaatagta
//
LOCUS       DAOOOL010000039        19334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_219_length_19334_cov_77.740287, whole genome shotgun sequence.
ACCESSION   DAOOOL010000039 DAOOOL010000000
VERSION     DAOOOL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19334
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219_length_19334_cov_77.740287"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5148)
                     /locus_tag="PKU82_04965"
     CDS             complement(<1..5148)
                     /locus_tag="PKU82_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOZ82260.1"
                     /translation="MKKILGLDLGTNSIGWALIEQNFDKKEGSVIGMGSRIIPMSQDI
                     KDEFGKGNSVSQTADRTKFRSVRRLRERHLLRRERLHRVLNVLGFLPEHYANDIDFEK
                     HFGKFKDETETKLVWKKNGKKPNGKDKFEFAFQSTFEEMLNEFKKSQPDLLNKKNKKG
                     EEFKIPYDWTIYYLRKKALTQKLAKEELAWLILNFNQKRGYYQLRGEEEEENQNKLVE
                     FHSLKVVEVKAEIKYILTLENNETYYRLSETPLLDWKDTNRDFVVTTYLETDGTVKKD
                     KDGKEKRSLFLKDDLVFIKKKTTKITKSTKNKNETVEEKSIKIIDVKEEKQNEIWYSL
                     ILENGWIYRRQSKVSLEDWKGKVREFIVTTDLNDDSSIKTDDEGKESRSFRAPKEDDW
                     TLIKKKTEDDLSKYIGEYKTKNGIDGTVGTYIYDNLLRKPNQKIKGKLVRTIERKFYK
                     YELREILKKQIVLQPELFSDDLYNDCVRELYKNNEAHQLQLNKRDFIHLFLDDIIFYQ
                     RPLRSQKSSIGNCSLEFRLHKINKKDDKGNSIKNEFEKDENGKDIEVKEYLKVIPKSN
                     PYYQEFRLWQWLYNLKIYKKEDDTNITADFITSNDDKITLFDFLNSRKEIEQKPLIEF
                     LVKQRELKNPANNNLNKKVQTALSKKIKEETEKYRWNYVEDKKYPCNGTGTQIRTRLE
                     RVTNVSVSFLTREIEQHLWHIIYSVTDKNEFEKALKSFSKRHQIDETSFVENFKNFPP
                     FISEYGSLSEKAIKKLLPLLRIGKYWNENAINDKIKKRANSISERLKSISKKEDIENI
                     SDDDIQKQILKSFYGLTDLTQGLQLYQASYLIYGRHSEASDLQSWNSVADLEKYLKEF
                     KQHSLRNPIVEQVVTETLRVVKDIWTHYGKGVENFFDEIHVELGREMKNTAEDRAVLT
                     KQVTSNENTNMRIKLLLAELKNDTTIEGVREYSPSQQEILKIYEDGVLNSNVEIPNDI
                     SEILKKFKESDIKKQPTKTEVNRYKLWLEQKYRSPYTGQMIPLSKLFTEAYQIEHIIP
                     QSRYFDDSFNNKVICEAAVNQLKDKQLGLEFIKNHSGQIIDLGQNQSVTLFTEQQYTD
                     FVKEHYDKNRSKRTKLLLDEIPDKMIERQLNDTRYISKFISSVLSNIVRANKDDDGVN
                     SKNLLPGNGKITSELRQDWGLNDVWNDLILPRFERMNELSQSNHFTATNKEGHTIPAI
                     PLELSKGFQKKRIDHRHHAMDALVIACATRDHVNLLNNQSAKSDNSRYDLQRKLRKVE
                     KWTDKEGKERDKFTDFYKPWSTFTQDTKNELEKIVVSFKQNLRVINKASNHYEKIING
                     KKVLVKQEGSNWAIRKPMHKDTVSGLVQLRKTKTVSLSVALENYNDIVDKTLRKHILS
                     LINQNYDKKKLTKYFKDNENKWNNRDISKLDIYYLENDNVASRVNLDISFNKERIEDT
                     ITDTGIQKILLKHLINYEGQTDEKGKAIAPETLAFTPEGIDEMNKNIITLNNGKSHQP
                     ILKVRTYEPKGNKFTIGETGNKKDKFVEAAKGTNLFFAIYEDENGNRSYETIPLNIVI
                     ERQKQGLCSVPEQNEKGHALHFHLSPNDLVYVPQVDENLDAINFGELTIEQSNRIYKF
                     TDGSGIMANFIPSKIAEVIFNINKLKQESLKINYPIQNEIGVGSQGSKNERAISGEQI
                     KSICIKLNIDRLGNITPSKKTNQKSTSSFVNEEHSIYYNKVTFNNLQQ"
     gene            5409..6230
                     /locus_tag="PKU82_04970"
     CDS             5409..6230
                     /locus_tag="PKU82_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOZ82261.1"
                     /translation="MSQDTSFTVYGHRGCRGLMPENTIPAFLKAIELNVLHLELDVVV
                     SADSQVVVSHEPWINNENCLMPDSTPLPDFKRAAYSIYKMPYEQVKGYDCGSKFYAKF
                     PGQHKMVVGKPLLSEVINTADTFCCNTKGVLPYYSIEIKSKKSWIGIFQPETKQLVDL
                     VMKVVQQYGEKERFVLQSFDPEVLRILHDDYPEYTLAALNETNRDVAAFFNELGFVTT
                     IYSPWYVLATAKAIAYCKQQEIKIIPWTVNDDKVFLKLKYSGVNGIITDYPDRYR"
     gene            6385..7191
                     /locus_tag="PKU82_04975"
     CDS             6385..7191
                     /locus_tag="PKU82_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ82262.1"
                     /translation="MLKSISLVAVLAVFASGAFALNPSKEYKVLPDKYGMTYKEEKIK
                     TPDGAILNAWMFELPKKSTNWIIMSGSGDGNMADNIEIANSFLSAGYNVAMYDYRGYG
                     SSSDFKIDPDLFIYPQFIVDLNAVCDYLRKSRAITKFNLYGKAIGAGLSIGVGANRTE
                     TQKVIADGPWIGLEVMKKKIKDKTGKEVVMPFGFDKNHEPMYAMEKPKGIKGLMVIVS
                     SQDNLINPTDVKQIKGVSETYVVKNSPSNAENFTIDKNVYFEKISKFLGN"
     gene            complement(7240..7590)
                     /locus_tag="PKU82_04980"
     CDS             complement(7240..7590)
                     /locus_tag="PKU82_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOZ82263.1"
                     /translation="MNFIIKLIISTLAVMMVSYILPGVHVQSFGIALMVACVLAFLNA
                     LIKPILVLFTLPITLITLGLFLLVINAIIIIITDRLIDGFEVSSFGIALLFSLILSIV
                     TSVMESLAGQNQRQ"
     gene            complement(7607..8107)
                     /locus_tag="PKU82_04985"
     CDS             complement(7607..8107)
                     /locus_tag="PKU82_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82264.1"
                     /translation="MYVSFDQLPDDSRIWIFQSDRLLSEEEIESINPILNFFISDWTA
                     HQQTLRASFTFMHNHFLIIGVDENMAIASGCSLDKVFHVVRACGQKINTDFLNRLHIA
                     AMIQNVITLHSIKQILDIAENRNNNEPLMIFSNIIETKSEMKNNWLIPIEKSWLAPKL
                     KSGSKI"
     gene            complement(8110..10362)
                     /locus_tag="PKU82_04990"
     CDS             complement(8110..10362)
                     /locus_tag="PKU82_04990"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOZ82265.1"
                     /translation="MGSDNNSDKNNLPSVSASGKGSKVVIDLEAEKKEISKRYRHLLR
                     ACKSDLDKKDKKLIRLAFDTALEAHKEMRRKSGEPYIYHPIAVAQIVAEEIGLRSAIP
                     VVCALLHDTVEDTYLTLEDIRDLFGPVAEKIIDGLTKIAGVFDHSSSLQAENFRKMLL
                     TLSVDVRVILIKLADRLHNMRTLGSMARDKQLKIASETAYLYAPLAHRLGLYNVKTEL
                     DDLALKYTEPEVYKTLAEKLRDSKKERDKFISEFIHPIKDELNKQGFKFEIKGRPKSI
                     HSIWEKMKKQGVTFEEIYDLFAIRIIIDSPQNQEKADCWKVYSIVTDSYRPNPDRLRD
                     WISTPKSNGYEALHTTVMSDQGKWVEVQIRSQRMNETAEKGFAAHWKYKESSQESALD
                     EWLTRIREMLENPDSNALDFVDDFKLNLFADEIFVFTPKGELKTLPVNSTALDFAYEI
                     HTDIGSRCIGAKINHRLVPISHKLNSGDQVEILTSDKQRPKEDWLSFVITAKAKGRIK
                     SSLKDEKRILGEQGKEIFDRKIKHLKIPVAEVNMNDILLYYKLPNVLELYYRIAMDAV
                     DLKQLREAVNYKDQRVRSQRTEPTLEELVTQARGSSDMLVIGENLNKIDYKLSPCCNP
                     IPGDDVFGFITINDGIKIHRVNCPNAMQLMSNYAYRIVKAKWTGQEQLSFLAGIKITG
                     MDDVGVVNNITKVISSELKVNMRSISIESNEGLFEGTIMLFVHDTEHLTKLMKKLSAL
                     SGILNVTRIT"
     gene            complement(10439..11209)
                     /locus_tag="PKU82_04995"
     CDS             complement(10439..11209)
                     /locus_tag="PKU82_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOZ82266.1"
                     /translation="MNLKLHRPLAFFDLETTGVVIGMDRIVELGILKILPDGTKTNKT
                     WRINPEMHIPEVVSKIHGIYDKDVADCPTFKEVANDVNHYLNNVDLAGYNSNRFDVPL
                     LVDEFLRAGIEFDIRGRKLIDVQHIFHKMEQRTLSAAYKFYCNQELIDAHSAEADVRA
                     TYEVLEAQLERYSDQLQPDAAFLHAFCNNSKNVDLAGRIVYDDKKREVFNFGKYKGRL
                     VTDVFKTDPSYYDWMLKGDFSRDTKQVVTALRLREREN"
     gene            complement(11211..12809)
                     /locus_tag="PKU82_05000"
     CDS             complement(11211..12809)
                     /locus_tag="PKU82_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOZ82267.1"
                     /translation="MRFVFAIIPILWLNIAIAQQSKKPVKKIVLGKDQVSEMKKAGSE
                     YFKSENYKTALPIYQQLQENDPNNAEFNYRLGLCYLNTNVNKKLAAGFLIKAAEGKDV
                     PKDVYYYTGRALLILEEIDDAVDAYEKFKIAHGGKVSPKLNLENNVEWCYHFRDMKKT
                     PIEVSFTNLGKLVNSPNNDYRPVCDMLGNVVYFSSNRKSNTGAIVDGYGEVISDGYFT
                     KKDTGYSKAKNLGPNINTMYYEEPLQLNSNSDKLLVFKEGGSISSPLYTSELNGKSFE
                     KAVAINKFPASKVDGACMTDDGKTIYFSADLKGGKGGKDIWMTTLNSKNEWSNPVNLG
                     DAVNTKYDEVNPWLFFDNQTLFFASEGHNSIGGFDIFFSTKPHESMDWSAAKNVGYPL
                     NTTDDNKYFSLTADCKTGFVSQTTEHGSGELDIVKFVCTNPIIKQEKVVCDFTFLKAD
                     GTFARDANCMIIRNDTGESSGVKTINGSNGKLYVYLGPGNYKVKVKGVKSGRADEDFI
                     ITGNEPKMQLVKTFKLTPPVKEGK"
     gene            complement(12816..14123)
                     /locus_tag="PKU82_05005"
     CDS             complement(12816..14123)
                     /locus_tag="PKU82_05005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOZ82268.1"
                     /translation="MSQVELKMPKMGESVAEATITKWLKKEGDRVEQDEPILEIATDK
                     VDSEVPSTAAGILVKQLYNEGDVVAVGKSIAIIGDSASATVPTSSVPKAEEVKIAAAP
                     VIATQAATVATTVSASSKFYSPLVRNIAQQENVSSAELDAITGTGAEGRVTKKDILQF
                     IENRKSGNGQITQTTQAPTVQPATTPQAKAPAATPMTDGDVEIIEMDRMRKLIADHMV
                     MSKHTSPHVTSFVEADVTNMVLWRNKVKNDFEKREKEKITFTPLFIEAIVRAIKDYPM
                     INCSLDGTKILLKKNINIGMATALPSGNLIVPVIKNADRMNLVGLTKSVNDLSGRARK
                     NKLSPDEIAGGTFTLTNVGTFGNVMGTPIINQPQVAIMAAGAIRKKPAVIETEFGDTI
                     GIRHMMFLSLSYDHRIVDGSLGGSFLRKVADYLEQFDVNRNLL"
     gene            14402..15901
                     /locus_tag="PKU82_05010"
     CDS             14402..15901
                     /locus_tag="PKU82_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82269.1"
                     /translation="MAQIQYPGIIDSTFGVNGKVTSSFNSFACVARAIAVQSDGKIVV
                     AGESNNGTFNRFAVARYLADGTIDNQFGTGGKITFSIGTGNSIARSIAIQNDGKIIVA
                     GNLENQTYSHFAMARLTTAGALDNQFGANGIVQTALDLGFDIAYGVAIVPSSGKIVLG
                     GVSFDTLANGEFAVARYNTNGTLDGGFGNSGKVRTPLGTQWDQANALAIEPGTEKIVL
                     AGRTLSTYALVKYNTNGTLDANFGLNGKAIYTIGSMSEIINTIYINPDGMIVAGGTTQ
                     TGSNTDMVLMRFLSNGQIDNNFGVSGISLFDLGGTDIIFSIAPQGDYLVGAGSSYVGA
                     AGDFVAMRFDTLGSFDLVFNNMIGYNITSFGNPSGANAVAVQQPDSKIILAGSAVVGT
                     VNNFAMARYTSDFTFVGLNEVNASHAKLQMMPNPASDFIAIEASTPYNRIEIKDITGK
                     KVEDVTPGSLIRSYSLDVKNYSSGVYTATVYFDSDIKTQKFIVTNTTNN"
     gene            15983..17353
                     /locus_tag="PKU82_05015"
     CDS             15983..17353
                     /locus_tag="PKU82_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ82270.1"
                     /translation="MLKKITAFATAFIVAGTAMAQKNLPSNFHMKKLGNGLEVLVIED
                     NSVPLVTIEICVKNGSFTEDSAFNGLSHLYEHMFFKANKAIPSQEKFLERVNELGISF
                     NGTTSEERVNYFFTLSNKLINEGLEFMNNAIRYPLFLEQEMKNENPVVDGEFQRAESN
                     PAFFLFQDFNKQMWGSNYVRKNAIGIHDVILSATPAKMNVIKDKYYYPNNSILVVAGD
                     VEHNAVFEKAEKMFGDWKPCDFDPFQKWPIPEFAPLVGQSKFLTVDKNAKMPIMMMGF
                     HGPDTRNDVKSTYAADVFSTILGLTSSEFQKTLVDGGYALQAGLGYQTCRYTGPIQLF
                     MVPNPMKMKSFYGKLMEQVNRFDAPDYFTDEQLETAKNKLIMDDIYNKEKTSAYVHTV
                     TFWWASASIDYYTTYNDMIKKVTRKDIADYVDKYIKGKPSVTGLLLNPKMKESLGINS
                     ADEILK"
     gene            17365..18696
                     /locus_tag="PKU82_05020"
     CDS             17365..18696
                     /locus_tag="PKU82_05020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ82271.1"
                     /translation="MNKLYKYFAIGAAMLSIHGVALAQATKEVNVDGLKVILRHTPKE
                     VISVRLYVKGGTANYTKDKEGVESLAFSIAMNGGTKKLDKVAFNDAAEKIGTSFEASS
                     GLDYGNLSMTCIKPNWNESWNLFADAVMNPAFDSKEFDLEKEKALSGAKQRESDADGS
                     LSNIAMEETFKGRNYAKDPQGSVASLEKITLADVISHYKNVIGKKRCYLVVVGDVNEA
                     DLVGKIKATLGKMAVGTAPVVEPKLVITKGSEVIVDRDIATNYLMGIMSAPSLSTEKG
                     VQMDLAMQMLYDRFFVELRTKRSLSYAPAAGYMRNAVSSPYSYIYITTTDPKKAIDVM
                     VSIIDSIKKTGFTEKELKDLRQGYLTNYYQKLETSEAQSGALGRSEATGSWKLDESLS
                     DMVNNVKLKDLNNTFNEYTAAIKWSYLGKKDAISKDDFKQTKHDKVLQSPY"
     gene            complement(18786..>19334)
                     /locus_tag="PKU82_05025"
     CDS             complement(18786..>19334)
                     /locus_tag="PKU82_05025"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217866.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOZ82272.1"
                     /translation="PILFAEDEEFKNVHFDKIPGLKPAFSKEGTVTAANASTMNDGAA
                     ALVLMSKEKAESLGLKPLGRIRSYADAEQAPEWFTTTPSKALPRAVDKAGLKMTDLEF
                     VELNEAFSVVALANMKEMNLDPAKVNVNGGAVALGHPLGCSGARILVTLLNVLKQNNA
                     KIGGAAICNGGGGASAMVIERM"
BASE COUNT         5748 a         3433 c         3351 g         6802 t
ORIGIN      
        1 ttgttgaagg ttgttgaatg taactttatt gtagtaaata gaatgttctt catttacaaa
       61 acttgatgtt gatttctgat tagttttttt tgaaggagta atgttgccta agcggtctat
      121 gttgagttta atacaaatac ttttaatctg ctcaccagat atagctcttt cattttttga
      181 gccttgtgat cctacaccaa tttcattttg aatagggtag ttaattttca aactttcttg
      241 ttttaattta tttatattaa aaataacttc agcaattttt gaaggtataa aatttgccat
      301 tatcccacta ccatcagtaa atttataaat tcgattcgat tgctctatcg tcaactcacc
      361 aaagttaatt gcatccagat tctcatctac ttggggcaca tacaccaaat catttggtga
      421 taaatggaaa tgtaaagcat gtcctttttc attctgttca ggaacggaac acaaaccttg
      481 tttttgtctt tcaatgacaa tgtttaatgg aatggtttca tagcttctat tcccattttc
      541 atcttcatag atggcaaaga aaaggttggt tcctttggca gcttctacaa atttatcttt
      601 tttgtttccg gtttcgccta tggtaaactt atttccttta ggctcatatg ttctaacttt
      661 taggattggt tggtgagatt taccgttatt caaagtaatg atgtttttat tcatttcgtc
      721 aattccttct ggtgtaaaag ctaaagtttc aggtgcaatt gcttttcctt tttcatctgt
      781 ttgtccttcg taattgatta aatgtttcaa taagattttt tgaataccag tatctgttat
      841 ggtatcttca attctttctt tattaaatga tatgtctaaa ttaactctgg atgcaacatt
      901 gtcattttcc aagtaatata tatctaattt agaaatatct ctattattcc atttattttc
      961 attgtctttg aagtattttg ttaatttctt tttatcataa ttctgattaa ttagagataa
     1021 aatatgctta cgtaaagttt tgtcaacgat gtcattataa ttctctaaag ccacacttaa
     1081 agaaactgtt tttgttttcc ttaattgaac cagtcccgaa acagtatcct tatgcatggg
     1141 ttttcttatc gcccaattcg atccttcttg ctttaccaaa acttttttac cgttgataat
     1201 tttctcgtag tggttgcttg ccttattaat tacccttaag ttttgcttga aactgacgac
     1261 aattttctct aattcgtttt tagtatcttg tgtaaatgtt gaccaaggtt tgtagaaatc
     1321 agtgaattta tctctttctt ttccttcttt gtcagtccat ttttctactt ttcgcagttt
     1381 acgttgcaaa tcatagcgac tattgtctga tttggcagat tgattgttca ataaattgac
     1441 atggtcacga gttgcacacg ctatgaccaa agcatccatt gcatgatgac ggtggtcaat
     1501 acgctttttt tggaaacctt tagacagttc caatggaata gcaggaatgg tgtgtccttc
     1561 tttattggtt gccgtaaaat ggttactttg tgacaattca ttcattctct caaaacgagg
     1621 cagaattaaa tcattccaaa catcattcaa cccccaatct tgtcgcagtt cggaagtgat
     1681 ttttccgttt ccgggcaaaa gatttttaga gtttactccg tcatcgtctt tgttggcacg
     1741 aacaatattt gataacaccg atgaaataaa cttgctgatg tagcgtgtat cattcaattg
     1801 gcgttctatc attttgtcgg gaatttcgtc caacaacaat tttgttcgtt ttgaacggtt
     1861 tttgtcgtag tgctccttta caaaatcagt gtattgctgc tctgtaaaaa gtgttacact
     1921 ttggttttgc cctaaatcaa taatttgtcc cgaatgattt ttaataaact ccaaaccaag
     1981 ttgtttgtct ttaagttggt ttacggctgc ctcacaaatt actttgttgt tgaaactatc
     2041 atcaaaataa cggctttgcg gaatgatgtg ttctatctga tatgcttcgg taaacaattt
     2101 agaaagcggt atcatttgcc ctgtgtaagg cgaacgatat ttttgttcta accataattt
     2161 ataacgattg acttccgttt ttgttggttg ttttttaata tcactttctt tgaatttttt
     2221 taatatttca gaaatatcat tcggaatttc aacattagaa tttaatactc cgtcctcgta
     2281 aatcttcaaa atctcttgct gggagggcga atattctcta acgccctcaa tagttgtgtc
     2341 gttttttaac tcagccaata acaacttaat acgcatgttg gtattttcat tactggtaac
     2401 ttgtttggtg aggactgcgc ggtcttcagc tgtatttttc atttcacgcc ctaactcaac
     2461 gtgaatttca tcaaagaaat tttctacacc ttttccgtaa tgtgtccaaa tatcttttac
     2521 cacacgaagt gtttcggtaa caacttgctc tacaatcggg tttcgtaacg aatgctgttt
     2581 aaattctttg agatactttt ccaaatcggc aaccgagttc cagctctgta gatcacttgc
     2641 ttcagaatgt cttccataaa tcaaataact tgcttgatat aattgtaagc cttgtgttaa
     2701 atcagttaaa ccgtaaaaac ttttcaaaat ttgtttttga atatcatcgt cagatatgtt
     2761 ttctatatct tctttttttg aaattgattt taatcgttca cttatggaat tggctctttt
     2821 tttaatttta tcgtttattg cattctcgtt ccaatattta cctatgcgaa gcaaaggcaa
     2881 taatttcttt attgcttttt ctgaaagact accatattca cttataaatg gtgggaaatt
     2941 tttgaaattt tctacaaacg aagtttcatc tatttgatgt cttttcgaaa atgattttaa
     3001 agctttttca aattcgtttt tatcagttac ggaataaatg atgtgccaaa gatgttgctc
     3061 aatttcgcgt gtaaggaaac taacagaaac gtttgttact ctttctaatc gtgtgcgaat
     3121 ttgcgttcct gttccgttgc acggatattt tttatcctca acataattcc atcggtattt
     3181 ttctgtttct tcttttatct ttttgcttag tgctgtttgt acttttttgt ttaaattatt
     3241 gtttgcagga tttttcagtt cccgttgctt aaccaaaaat tcaatcaaag gcttttgctc
     3301 aatttctttt ctgctgttca aaaaatcaaa aagagtaatt ttatcgtcat tgcttgtaat
     3361 aaaatcagcc gtaatatttg tatcatcttc tttcttgtat attttcaggt tgtacaacca
     3421 ttgccacaag cggaactcct gatagtacgg attggatttc ggaatgactt ttaagtattc
     3481 ttttacttca atatcttttc cattttcgtc tttttcgaac tcatttttta ttgaatttcc
     3541 cttatcgtct ttcttattga ttttatgtaa tcgaaattcc aacgaacaat taccaatact
     3601 tgatttttgg cttcgcaaag ggcgttgata aaaaataatg tcatctaaaa acaaatgaat
     3661 aaaatctcgt ttatttagtt gcagttgatg tgcctcattg tttttataca actctcttac
     3721 acaatcatta tacaagtcat ctgaaaacaa ttcgggctgc agtacaattt gctttttaag
     3781 aatttctctt aattcatatt tgtagaactt gcgttcaatc gtacgaacta atttcccttt
     3841 aattttttga ttgggctttc gaagcaaatt atcgtaaatg tatgtgccga cagttccgtc
     3901 aattccattt tttgttttat attctccaat gtatttactt agatcatctt cggttttctt
     3961 ttttattaaa gtccaatcat cttctttcgg tgcacggaaa ctgcgacttt cttttccttc
     4021 gtcatctgtt tttattgaac tgtcatcgtt taaatcggtg gtaacaataa attcacgcac
     4081 tttgcctttc caatcctcta atgaaacttt actttgtctt cgataaatcc agccgttttc
     4141 taatattaac gaataccaaa tttcattttg tttttcttct tttacatcaa taatctttat
     4201 tgatttttct tcaaccgttt catttttatt ttttgttgat ttagtaattt ttgttgtttt
     4261 ctttttgata aaaactaaat catctttcaa aaacaaacta cgtttttctt ttccgtcttt
     4321 atcttttttt actgttccgt cagtttccaa ataggttgtt acaacgaagt cgcggtttgt
     4381 atctttccaa tccaatagag gcgtttcgct taaacggtag taggtttcat tgttttccaa
     4441 agttaagatg tattttattt ccgcttttac ttctactact ttcaacgaat gaaattctac
     4501 caacttattt tgattttctt cctcttcttc accacgcaac tgatagtaac cacgtttttg
     4561 attgaaattt aaaatcaacc aagccaattc ttcttttgct aatttttgtg tgagtgcttt
     4621 ttttcgcaaa taataaatcg tccaatcgta aggaatttta aattcttcac cttttttgtt
     4681 tttcttattc aataagtcgg gttgtgattt tttgaactcg ttcaacattt cttcaaatgt
     4741 tgattgaaaa gcaaattcaa acttatcttt tccattcggt tttttcccat ttttcttcca
     4801 aaccaatttg gtttcagttt cgtctttaaa ttttccgaaa tgtttttcaa aatcaatgtc
     4861 atttgcataa tgttcaggta gaaaacctaa tacatttaat actcgatgta acctttctct
     4921 tcttaaaaga tgtcgttcac gtaatcttct tacacttcta aatttcgttc tatctgctgt
     4981 ttgtgaaact gaatttcctt ttccaaattc atctttaata tcctgagaca tcggaatgat
     5041 tctgcttccc attccaatga cactaccttc tttcttatca aaattttgtt ctatcaatgc
     5101 ccagccaatt gagtttgtgc caagatctag tcctagtatt tttttcattt cagtacttat
     5161 aaatatgatt ttgcgaataa aattaaggaa tattttttaa attaactgct aaaatttgga
     5221 gggaatcaaa ttttaaacta attttgtttt tgaaagcaaa tcacaataag gattattccg
     5281 ttgtgaaaac atctactacg gggcactctc aagattgcct cgtttttatt tttagttaat
     5341 aatgaactgt tattgtgcgt attattctat ttttaatttt tcttttttct ttatttagta
     5401 tccatctcat gtcacaagac acatctttca cagtttatgg gcatcgtgga tgtcgtgggt
     5461 taatgcctga aaatacaatt cctgcatttc tgaaagctat tgagctaaat gttttgcatc
     5521 ttgaattaga tgttgttgta tcggcagatt ctcaggtagt ggtttcgcat gagccctgga
     5581 taaataacga aaactgcctg atgcctgaca gtacaccatt gccggatttt aaaagagcag
     5641 cttacagtat ttacaagatg ccttatgaac aggtaaaagg ttatgattgc ggtagtaagt
     5701 tttatgcaaa gtttcccgga caacataaaa tggttgtagg taaacctttg ctttctgaag
     5761 taattaacac tgctgatact ttttgttgta atacaaaagg agtgttgcca tattattcca
     5821 ttgaaataaa aagtaagaag agttggatag gcatatttca gcctgaaaca aagcagttag
     5881 ttgatttggt gatgaaggtt gtgcaacagt atggcgagaa agaaagattt gtattgcagt
     5941 cattcgatcc tgaagtgttg cgtattttgc atgatgatta tcctgaatat actttggcag
     6001 ctttgaatga aacgaatagg gacgtggcgg ctttttttaa tgaacttggt tttgtgacaa
     6061 caatttacag cccttggtat gtattggcaa ctgcaaaggc aatagcatat tgcaaacagc
     6121 aagaaattaa aattattccc tggacggtga atgatgacaa agtattttta aagctgaaat
     6181 acagcggtgt taatggcatt attacagatt atcccgatcg ctatcgttaa gttattctaa
     6241 gtgagaaata attgcagata tttgtttttt tgccgcaaag tagattttat cagcaatgaa
     6301 atggcaagaa ttgttaaaaa attaattact tattcatgga tagtgtactt atttttgaag
     6361 cttaaattta acaatcaaat tacgatgctt aaatcaattt cattagttgc cgtattggcg
     6421 gtatttgctt caggtgcttt tgcactaaat ccttcaaagg aatataaggt acttcctgat
     6481 aagtacggaa tgacttataa agaagaaaaa attaaaactc ctgatggtgc aattctcaat
     6541 gcatggatgt ttgagttgcc gaaaaaatct accaactgga taattatgag tggcagtggt
     6601 gatggcaata tggccgacaa tattgaaatt gctaattcat ttttaagtgc aggatataat
     6661 gtagccatgt atgattatcg tggttatggc agcagcagcg attttaaaat tgaccccgat
     6721 ttatttatct atcctcagtt tattgttgac cttaatgctg tttgtgatta cttacgtaag
     6781 tcgcgcgcca tcacaaagtt taatttatat ggtaaagcta ttggcgcagg actttcaatt
     6841 ggtgttggtg caaacagaac agaaacacag aaagttattg ccgatggtcc atggattggc
     6901 cttgaagtaa tgaagaaaaa aataaaagat aaaacgggta aagaggtggt aatgcctttt
     6961 ggctttgaca aaaatcatga gccaatgtat gctatggaaa aacctaaagg cattaaggga
     7021 ttaatggtta ttgtttcatc tcaggataat ctgataaatc caacagatgt aaaacaaatt
     7081 aaaggtgtca gtgaaaccta tgttgtaaaa aattctccat ccaatgcaga aaattttaca
     7141 atcgataaaa atgtttattt tgaaaagatt tcaaagtttt taggaaacta atgtttattt
     7201 gacagcacat aagccggcac ttttaaagtt gccggttttt tattgtcttt gattttgtcc
     7261 ggcaagcgac tccattacag aagtaacaat gcttaatatc aaagagaaca gaagtgcaat
     7321 accaaaactg cttacttcaa atccatcaat tagcctgtcg gttatgataa tgatgattgc
     7381 attaatgacc agcaaaaata aacctaatgt gatgagtgta ataggtagtg tgaaaagtac
     7441 aagtattggt tttatcaatg catttaaaaa tgctaagaca catgctacca taagtgcaat
     7501 gccaaaactt tgaacatgca caccgggcag aatgtatgac accatcataa ctgccagagt
     7561 agagataatg agtttgatta taaaattcat aaaatagttt taataattaa attttacttc
     7621 ctgattttaa ctttggtgca agccatgatt tttcaattgg tattagccag ttatttttca
     7681 tttcactttt ggtttcaatg atgttgctga atatcattaa tggctcatta ttgtttctgt
     7741 tttctgcaat atccaatatt tgttttatag agtgaagcgt aataacattt tgaatcattg
     7801 ctgcaatgtg cagacggttt aaaaagtcag tattaatttt ttggccacat gcacgaacaa
     7861 catgaaaaac cttatcgaga gaacagccgc tggcaatagc catattttca tcaactccaa
     7921 taatcagaaa atgattatgc ataaaagtga atgaggctct tagagtttgt tgatgtgctg
     7981 tccagtcact gataaaaaaa ttaagtattg gattgataga ttcaatttct tcttctgata
     8041 ataatctatc cgactgaaaa atccagatac gggagtcgtc cggtaattgg tcaaaagata
     8101 cgtacatgat taagtaattc gagttacatt taaaataccg ctaagtgcag aaagcttttt
     8161 catcagttta gtcagatgtt cggtgtcgtg aacaaaaagc atgatggttc cttcaaacaa
     8221 accttcatta ctttcgattg agatagaacg catattcact ttcagttcgc ttgaaataac
     8281 cttggtaata ttatttacaa cacctacatc atccattcct gtaatcttta tacctgccaa
     8341 gaaagaaagc tgctcctgac cggtccattt tgcttttaca attctataag catagttgct
     8401 catcagttgc atggcattgg ggcagtttac acgatgtatt tttattccgt cattaatggt
     8461 gatgaaacca aaaacgtcat caccaggaat aggattacag catggtgaaa gtttataatc
     8521 tattttattc aggttttcac caataacaag catatcggac gagcctcgtg cctgtgttac
     8581 caactcttct aaagtaggtt ctgtacgctg actgcgcaca cgttggtctt tataattaac
     8641 ggcttcgcgc aactgtttca gatcaacagc atccatggct atacgataat acaactccaa
     8701 aacattcgga agtttgtaat agagaagtat atcattcatg ttaacttctg ctacaggaat
     8761 tttaagatgt tttattttac ggtcaaatat ttccttgcct tgttcaccaa gaattctctt
     8821 ttcatctttt aatgatgatt ttattctgcc tttagctttt gcagtaatca caaaacttaa
     8881 ccagtcttct tttggtcgtt gtttgtcgga ggttaaaatt tcaacctgat caccactatt
     8941 cagtttatgt gaaatgggta ctaaacgatg attaattttt gctccgatac atctgctgcc
     9001 tatatcggtg tgtatttcat aagcaaagtc taatgcagta ctgttgacgg gtaaagtttt
     9061 taattcacct ttaggtgtaa aaacaaaaat ttcgtctgca aagagattaa gcttaaagtc
     9121 atcaacaaaa tcaagagcat tactgtcggg gttttcaagc atttctctga tacgtgtaag
     9181 ccattcatcc aaggccgact cttgcgaaga ctctttatat ttccaatgtg ctgcaaaacc
     9241 tttttcagca gtttcattca ttctttgtga acgaatttgt acctcaaccc atttgccctg
     9301 atcactcatc actgttgtgt gcaatgcttc atagccattc gattttggtg ttgatatcca
     9361 atcgcgcagc ctgtcagggt ttggtcggta actatcagta acaatagaat acactttcca
     9421 gcaatcagct ttttcctgat tttgtggtga gtcaataatt attcttattg caaataggtc
     9481 ataaatttct tcaaaggtta ctccttgctt tttcatcttc tcccagatgg aatgaataga
     9541 ttttggcctt cctttaatct caaatttgaa accttgttta tttaactcat cttttatcgg
     9601 atgaataaat tccgaaataa atttgtcgcg ttcttttttt gagtctctta gtttttcggc
     9661 aagtgtttta tatacttcgg gttctgtata tttcaatgcc agatcgtcaa gttctgtttt
     9721 tacattataa agcccaagac gatgtgccaa aggtgcatat aggtatgctg tttcagaagc
     9781 tattttcaac tgcttgtcac gtgccatact accaagtgta cgcatgttgt gaagtctgtc
     9841 ggcaagtttg atgagaatta ctctgacgtc aacacttaat gtaagtaaca tctttctgaa
     9901 attctctgcc tgcaaagagc ttgaatgatc aaaaactcct gcaatttttg tcagcccatc
     9961 aataattttt tctgcaacag ggccaaacaa atcacgtata tcttctaatg taagataagt
    10021 atcttcaaca gtgtcatgca gtaaagcgca aactacaggg atggcagaac gaagtccgat
    10081 ttcttcagca acaatctgtg caacggcaat cgggtgatag atatatggct ctcctgattt
    10141 cctgcgcatt tccttatgag cttcaagagc agtatcaaat gcaagcctga taagtttttt
    10201 atctttttta tctaaatctg atttgcatgc tctaagtaaa tgcctatagc gtttactgat
    10261 ttcttttttc tcagcttcaa ggtcaataac aactttgctg cctttacctg aagcagaaac
    10321 agaaggaaga ttatttttat ctgaattatt gtcggaaccc atcagcataa aagtacagat
    10381 ttttatatta cctgcaaatt ttaatcagca gaaataatca aacaggtcaa acatatattt
    10441 agttttctct ttcgcgtaat cgcaatgccg ttacaacttg tttagtatct ctcgaaaaat
    10501 cgcctttaag catccagtca taatagctag gatcagtttt aaaaacgtca gtcaccaagc
    10561 gtcctttgta ctttccaaaa ttaaatactt cgcgcttttt gtcatcataa actatacgcc
    10621 cggccaaatc aacattctta ctgttattac aaaaggcgtg aagaaaggct gcgtcaggtt
    10681 gtagctgatc gctataacgt tctaattgcg cttccagaac ttcataggta gctctcacat
    10741 ctgcttcggc agaatgggca tctattaact cctgattgca gtagaattta tatgcagcac
    10801 tcaatgtgcg ttgttccatt ttatgaaaga tgtgttgtac atcaattaac tttcgtcctc
    10861 gtatgtcaaa ttcaatacct gctcttaaaa attcatcaac cagcaagggt acatcaaatc
    10921 ggtttgaatt gtatccggca aggtcaacat tattgagata gtgatttaca tcgtttgcca
    10981 cttctttaaa agtagggcag tcggccacat ctttatcata aatgccatga atttttgata
    11041 ccacttcagg aatgtgcatt tcaggattta tacgccatgt tttgtttgtc tttgttccat
    11101 caggtagaat ttttaaaatc cccaattcaa caattctgtc catgcctata actacgcctg
    11161 tagtttcaag atcaaaaaaa gcaagtggtc tgtgcagttt cagattcatt ttattttcct
    11221 tctttaacgg gaggtgtcaa tttaaaagtt tttaccaatt gcattttagg ttcattgcct
    11281 gtgatgataa aatcctcatc agcacgacct gactttacgc cttttacttt aactttataa
    11341 ttacccgggc cgaggtaaac ataaagtttg ccgtttgagc cattaattgt tttcacacct
    11401 gatgattcac ctgtatcgtt tcttataatc atacagttgg catcacgtgc aaaagtacca
    11461 tcagctttta aaaaagtaaa atcacataca actttttctt gtttaatgat tgggttggtg
    11521 caaacaaatt ttacaatatc caactctccg gaaccatgtt cagtagtctg acttacaaag
    11581 ccggttttac aatcagctgt taaagaaaaa tatttattat cgtcagtagt atttaatgga
    11641 tagcctacat ttttagcagc actccagtcc atagattcat gcggctttgt actgaagaaa
    11701 atatcaaatc cgccaatact attatggcct tcggaagcaa aaaacaaagt ctgattatca
    11761 aaaaacaacc aagggtttac ttcatcatat tttgtattta cagcatcacc aagattcacc
    11821 ggattagacc attcgttttt actgtttaaa gttgtcatcc aaatatcttt accaccttta
    11881 ccacctttaa ggtctgcaga aaaatagatt gtttttccat catctgtcat gcaggcacca
    11941 tcaacttttg aagcaggaaa tttgtttata gcaactgctt tctcaaatga ttttccattg
    12001 agttctgatg tatataatgg agaagaaatg ctgccacctt ctttaaaaac caacaatttg
    12061 tcgctgttgg aatttaattg caatggttcc tcataataca tggtgttaat gtttggtcca
    12121 agattttttg ctttactgta gcctgtatct tttttagtaa aataaccgtc tgaaataact
    12181 tctccgtaac catcaacaat ggcacctgtg ttgctttttc tatttgagct gaaataaact
    12241 acattaccta acatatcaca aaccggtctg taatcgttgt taggtgaatt aaccaattta
    12301 cccagattag taaaacttac ttcaattggt gttttcttca tatcgcgaaa gtgatagcac
    12361 cattccacat tgttttcgag attcagtttt gggctaactt ttccaccatg agctatttta
    12421 aatttctcat aagcatctac agcatcatct atttcttcaa gaatgagaag tgcccgacca
    12481 gtatagtaat aaacatcttt tggaacatct tttccttcag cggcttttat cagaaaacct
    12541 gccgctaatt ttttatttac attggtattt aaatagcaaa gaccaagtct gtaattaaac
    12601 tcagcattgt ttggatcgtt ttcctgaagt tgctgataaa taggtaatgc agtcttataa
    12661 ttctcactct tgaaatattc agaacctgct tttttcattt cggaaacctg atcttttcct
    12721 aaaacaattt tttttacagg tttctttgac tgttgcgcta ttgcaatatt cagccagagt
    12781 attggaataa tggcaaatac aaatctcata acaaattata ataaatttct gttaacatca
    12841 aactgctcaa gatagtctgc taccttacgg agaaaactac ctccaagact tccatcaact
    12901 attctatggt cataagataa agagagaaac atcatgtgac ggatacctat agtatcgcca
    12961 aattcagttt caattacagc aggtttttta cgaatggcac ctgcagccat aatagctact
    13021 tgtggttgat tgataatggg tgtacccata acatttccaa aagtgcctac attggtcaat
    13081 gtaaaagttc cacctgcaat ttcatcaggc gaaagtttgt ttttacgcgc tctgccactc
    13141 aaatcattta ccgactttgt tagcccgaca agattcattc tgtcggcatt ttttattacc
    13201 ggaacaatca ggttgccact tggtaatgca gttgccatac caatattaat gttcttcttg
    13261 agaagtattt tagtgccatc aagtgaacag ttaatcattg gataatcttt tatggcacgc
    13321 acaattgctt caataaacaa tggagtgaaa gtgatttttt ctttctctct tttctcaaaa
    13381 tcgtttttaa ctttattgcg ccacaaaacc atattggtga catcagcctc tacaaatgat
    13441 gtcacatgcg ggctggtatg ttttgacatc accatgtggt ctgcaattaa cttccgcatt
    13501 ctgtccattt caataatctc tacatcgcca tcagtcattg gagttgcagc aggcgcttta
    13561 gcttgcggtg ttgttgcagg ttgcacagta ggcgcttgag tagtttgtgt tatttgtcca
    13621 ttacctgatt ttctgttctc aataaattga agaatatctt tttttgttac acgaccttca
    13681 gcaccggttc cggtaattgc atccaactct gcagatgaaa cattttcctg ttgtgcaata
    13741 tttcttacta gtggagaata gaattttgaa gatgcactaa cagtggtagc cacagttgca
    13801 gcttgagttg caattactgg agccgctgca attttaactt cttctgcctt aggtactgat
    13861 gaggttggta ctgtagctga cgcagaatca ccaataatag caatggattt tccaacggca
    13921 acaacatcgc cttcattata taattgttta actaaaattc ctgctgctgt ggaaggaact
    13981 tcagaatcaa ctttatctgt tgcaatttca agaataggtt catcctgctc aactctatct
    14041 ccttcttttt ttaaccattt ggtaattgtt gcctcagcaa cgctttcgcc cattttaggc
    14101 atcttcagtt caacttgtga catagtattt tacgtgcaaa attttattgc tcaaagttac
    14161 agatttaatt aaattgggca gggcagttta ttatatttta ttgacatttt gaatcatcaa
    14221 agatcaagaa cagactatgc caaattgctg attacactta agctataaga aataattgcc
    14281 aactaacaat tgcctttcag gctaaattat atatttgcat cataagtaaa aaacattcag
    14341 aatgagaaaa tttctacaca catgcctcat tgcaggtctt tcttttttta cactacaatc
    14401 aatggctcag attcagtatc ccggtattat tgattctacc tttggtgtaa atggtaaagt
    14461 aacttcatcg tttaatagtt ttgcttgtgt tgccagagca attgctgtgc agtcagatgg
    14521 aaaaattgtt gtagctggtg aaagtaataa tggaactttt aatcgttttg cagttgcccg
    14581 ttatcttgct gatggtacaa ttgataatca gtttggaaca ggggggaaga taactttttc
    14641 tatcggcaca ggtaacagta tagcgcgctc cattgccata caaaatgatg gtaaaattat
    14701 tgttgccggt aatcttgaaa atcaaacata ttcgcatttt gctatggcca gattaaccac
    14761 agcaggtgca cttgacaatc agtttggtgc taacggtatt gtgcagacag cattagattt
    14821 aggattcgat attgcttacg gtgtagcaat agttccttca agtggtaaaa ttgttttggg
    14881 aggtgtttct ttcgatacat tggctaatgg cgaatttgct gttgctagat ataatacaaa
    14941 tggaacctta gatggtggct ttggaaacag tggcaaggtt agaacaccat taggcacaca
    15001 atgggatcag gcaaatgcat tggcaataga acctggaaca gaaaagattg ttcttgcagg
    15061 cagaacgctt tcaacttatg ctttggttaa atataatacc aacggtacat tagatgcgaa
    15121 ttttggatta aatggaaaag caatctatac cataggctcg atgtcagaaa tcattaatac
    15181 aatatatata aatcctgatg gaatgatagt tgccggaggc acaacacaga ccggttcaaa
    15241 cacagacatg gtgctgatgc gtttcttaag taatggtcag attgataata actttggtgt
    15301 aagcggcatt tctctttttg atttaggggg aacagatatt attttttcta tcgctcctca
    15361 gggtgattat ctggtaggtg caggttcaag ttatgtaggc gctgccggtg attttgttgc
    15421 tatgcgtttt gatacattag gttctttcga tttggttttt aacaacatga ttggttataa
    15481 cataacttca tttggtaacc cttccggagc aaatgctgtt gcagtacagc aacctgattc
    15541 taaaattatc cttgccggat ctgccgttgt aggtacggtt aataattttg ctatggcgcg
    15601 atatacaagt gactttacat ttgttggctt aaatgaagta aacgcttcac atgctaaatt
    15661 acagatgatg ccaaatcctg cctcagattt tattgccatt gaggcatcaa cgccatacaa
    15721 tcgtattgaa attaaggata ttactggaaa aaaggttgaa gacgttactc ccggaagttt
    15781 gattcgcagc tattcattag atgtaaaaaa ctattcatca ggagtttata cagcaactgt
    15841 ttattttgat tcggacatta agactcagaa attcattgtt accaatacca caaacaatta
    15901 aatttaatta ataacataac ttttttttaa ccatcacggg ctatagaaat agcgttctta
    15961 gcctactatt aaatgtataa ctatgcttaa aaaaatcaca gcctttgcta ctgcctttat
    16021 tgtagccggt acagcaatgg cacaaaaaaa tctgccttcc aactttcata tgaaaaagtt
    16081 gggcaatggc cttgaggtct tggtcattga agacaactct gttccattag tcactattga
    16141 aatttgtgta aagaatggat cttttactga agattcggcc ttcaatggat tgtcacacct
    16201 ttacgaacac atgttcttta aagcaaacaa ggcaattccg tcacaagaaa aatttcttga
    16261 aagagtaaat gaactcggta tatcatttaa cggtactacc tctgaagagc gtgtgaacta
    16321 ctttttcaca ctaagtaaca aactcatcaa tgaaggtctt gagtttatga ataatgctat
    16381 tcgttatcca ttatttctgg agcaggaaat gaagaacgaa aatcctgttg ttgatggtga
    16441 atttcagcgt gcagaatcaa atcctgcctt ttttctgttt caggatttta acaaacaaat
    16501 gtggggttca aattatgtgc gtaaaaatgc catcggtatt catgacgtaa ttctttctgc
    16561 tacaccagca aagatgaatg taataaaaga taaatattac tatcccaata actctatttt
    16621 agttgttgcc ggtgatgtgg agcacaatgc cgtttttgaa aaagcagaga aaatgtttgg
    16681 tgactggaaa ccttgtgatt ttgatccgtt tcaaaagtgg cctatacctg agtttgcacc
    16741 attagtaggc cagtcaaagt ttttgaccgt tgataaaaat gcaaaaatgc caattatgat
    16801 gatgggattt catgggcctg atacaagaaa tgatgttaag tcaacttatg cagccgatgt
    16861 gttttctact attcttggac tgacatcttc tgaatttcaa aaaactttgg tagatggtgg
    16921 ttatgctctt caggcaggtt taggctatca gacttgtcgc tacacaggac caattcagtt
    16981 atttatggtt cctaatccta tgaagatgaa atctttttat ggtaagttga tggagcaggt
    17041 aaatcgtttt gatgcacctg attattttac tgacgaacaa ttagagactg ctaagaataa
    17101 actcatcatg gatgatattt ataacaaaga aaaaacatca gcctatgtgc atactgttac
    17161 attttggtgg gcatctgcaa gcattgacta ctatactacc tacaatgaca tgataaagaa
    17221 agttaccaga aaagatattg cagactatgt agataaatat atcaaaggaa agccttctgt
    17281 aacaggtctg ctcttgaatc ctaaaatgaa ggagtcttta ggaattaatt ctgctgatga
    17341 aattttaaaa taaaaaatcg aactatgaat aagttatata aatattttgc aataggtgca
    17401 gcaatgttat caatacatgg tgttgcgctt gcacaagcaa caaaagaagt taatgttgat
    17461 ggtctgaaag taatattacg ccatacgcca aaagaagtta ttagtgtaag actttatgta
    17521 aaaggaggaa ctgcaaatta tacgaaggat aaggaaggtg tagagagcct tgcattttct
    17581 attgctatga atggcggtac caagaaattg gataaagttg cctttaatga tgcagctgaa
    17641 aaaattggta catcattcga agcatcatca ggactggatt atggaaacct gagtatgaca
    17701 tgcattaaac ctaattggaa tgaatcgtgg aatctttttg ctgatgcagt aatgaatcca
    17761 gcttttgact ctaaagagtt tgatttagaa aaagaaaaag cattgtcagg agctaagcaa
    17821 cgcgagtctg atgccgatgg ttctttgagc aatatcgcta tggaagaaac ctttaaaggc
    17881 agaaactatg ctaaagatcc acaaggctct gttgcaagcc ttgagaaaat aaccttggct
    17941 gatgttataa gtcattacaa aaatgtaatt ggaaaaaagc gttgctactt agttgttgta
    18001 ggtgatgtta acgaagcaga tttagtgggt aaaataaagg cgacattagg aaaaatggca
    18061 gtaggcacag caccggttgt ggaacctaaa ttggtaataa caaaaggaag tgaagttatt
    18121 gttgaccgtg atattgccac aaactatctg atgggaatta tgagtgctcc ttctttgtca
    18181 acagagaaag gtgttcagat ggatttagca atgcaaatgc tttacgacag attttttgtt
    18241 gagcttagaa caaaacgcag cttgtcttat gcaccggctg ccggatatat gcgcaatgca
    18301 gtttcatcac catattcata tatctacatc acaactacag atcctaaaaa agctattgat
    18361 gtaatggttt caattattga ctctataaag aaaacaggtt ttacagaaaa agagttgaag
    18421 gatttacgtc agggttatct gactaactat tatcagaaat tagaaacatc tgaggcacag
    18481 tcaggtgcct tgggtcgtag tgaagcaaca ggctcatgga aattagatga gtccttatct
    18541 gatatggtta acaatgttaa gctgaaagat ttgaataata cattcaatga atatacagca
    18601 gcaataaaat ggtcgtatct tggtaagaaa gatgcaattt ctaaagatga ttttaagcag
    18661 acaaaacacg ataaagtgtt gcaaagcccg tattgatatt ttgaaatagg ttagtgactt
    18721 ctgctaacaa aaagaggcta tctctaattg agatagcctc ttttattgct aatattaaag
    18781 tagtattaca ttctttcaat aaccatagca ctggcaccgc caccgccatt gcaaattgct
    18841 gcaccgccaa tttttgcatt gttttgtttt aaaacattta ataacgtaac caatattctt
    18901 gcacctgaac atcctaatgg atgacctaat gcaactgcac caccattgac atttactttt
    18961 gcaggatcaa gattcatttc tttcatgttg gctaaggcaa ctactgaaaa ggcttcgtta
    19021 agttctacaa actcaaggtc agtcattttt aatccggctt tatctactgc tcttggtaat
    19081 gcttttgatg gagtagtggt aaaccattca ggcgcctgct ctgcatcggc ataactgcgg
    19141 atacgcccaa ggggtttaag tcccaatgac tctgcttttt ctttactcat taaaaccaat
    19201 gcagctgcac catcattcat ggttgaagca ttggcagcag ttactgttcc ttctttgctg
    19261 aaagccggtt ttaatcccgg aatcttatcg aaatgaacgt ttttaaattc ttcgtcctct
    19321 gcaaaaagaa tagg
//
LOCUS       DAOOOL010000040        19289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_221_length_19289_cov_146.076115, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000040 DAOOOL010000000
VERSION     DAOOOL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221_length_19289_cov_146.076115"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKU82_05030"
     CDS             <1..668
                     /locus_tag="PKU82_05030"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOZ82273.1"
                     /translation="EAGFYFPSWIFSWPMILIVIGIFLGVKHAFRNMAWMVLVLIGGL
                     FLIRNSFPIFEYRNFIWPVVIILLGLFIILKPSNRFRRACRRGRFHPRHPQRQFGNYT
                     GTPSSGEDFLEAVAVFGSIKKNIISKDFKGGEVSAVFGGAEINFMQADIQGRVELEVN
                     QVFGGTKLIVPAHWEIKSEIAAVLGSVEDKRPLRSASLTDEKKVLILRGTTVFGGIDI
                     NSY"
     gene            704..1042
                     /locus_tag="PKU82_05035"
     CDS             704..1042
                     /locus_tag="PKU82_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HOZ82274.1"
                     /translation="MYFIAKVIYQIVINGSKNNQFDEQLLIIKAQTVADAYLKARAIG
                     AEKQHEFINDRKNPVMWKMIDVADIIPVESPEHGSVVYSQVIEEDSESFIDYIKRKAL
                     TLQTQSLLFA"
     gene            1049..2074
                     /locus_tag="PKU82_05040"
     CDS             1049..2074
                     /locus_tag="PKU82_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ82275.1"
                     /translation="MIIEKIKNSIDQIVWWLCWYFFVAALVHQFGFDWCLSFADSTVM
                     LLLLLLTSCSTYFAFNKFQKTGNRFFVILWNIALSWISVFILGLVLRYIYVDDLKYLT
                     FLDSSMVVRFTWFFIMNLLVTVIYGLQVNILEQKKLEAHNNENANLMKDAELSGLRLQ
                     LQPHFLFNSLNSINALIGTEPAKARSMVLQLSEFLRGTLQRDNKKLISLQEEISHLKI
                     YLEIEKVRFGHRLITQIEIPENCLNMMVPPLILQPIVENAIKFGLYDTTGQVTISLKV
                     SCMESHLRIFVANPFDSDTAASQKGTGFGLDAVQRRLYLLYARNDLLKTEKENSIFIT
                     QIDIPQI"
     gene            2071..2805
                     /locus_tag="PKU82_05045"
     CDS             2071..2805
                     /locus_tag="PKU82_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOZ82276.1"
                     /translation="MIKAIVIDDEPLARSLVLEFLKAFPNIEVVQECNDGFEGLKAIQ
                     QFQPQLIFLDIQMPKINGFEMLELIENPPGVIFTTAFDNYAIKAFEAHAIDYLLKPFS
                     EERFNKAVAKFLATNNKAPAMDLVSAESNILPDEKNRIVVKDGSKITIIPVDDVIYIE
                     AYDDYVKVHTQNGFKLKKKTMSYYENTLDNSKFLRVHRSYIVKLSMITRIEQYEKTSH
                     IALLKNGQKVPLSKQGYARLKSVLGI"
     gene            complement(2807..3223)
                     /locus_tag="PKU82_05050"
     CDS             complement(2807..3223)
                     /locus_tag="PKU82_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82277.1"
                     /translation="MIRICMLYIICFSAVNCQAQCLKLKTNITAEDNLYDSIVVVIAD
                     ESGNVNRLNTTKENRLKLKLNNHYLITIIKPDAVAYKFEVNTHLPVKLSKKNYSYSTN
                     IFLEKLNPADANAQKEKACKTRMTISFNKDKKAFGF"
     gene            complement(3220..3951)
                     /locus_tag="PKU82_05055"
     CDS             complement(3220..3951)
                     /locus_tag="PKU82_05055"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOZ82278.1"
                     /translation="MTACKLSVNINKIATLRNARGGTIPNVVKAAIDIERFGAEGITV
                     HPRPDERHIRFRDVYELKECVTTEFNIEGYPSEGFLKLVESVKPHQVTLVPDSPDVLT
                     SNAGWNAIREQAFLKNVIERLKQKCLRVSLFMDTDLKQIDAAATTGTDRIELYTERYA
                     SGYEKSAQQAIADYQKAASHASTLNMGVNAGHDLNLNNLNYFIKNIPQVIEVSIGHAL
                     ISDALYFGLQNTVQMYKHQIKSARE"
     gene            4017..4679
                     /locus_tag="PKU82_05060"
     CDS             4017..4679
                     /locus_tag="PKU82_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOZ82279.1"
                     /translation="MNTKAYIKDFIPPLKSSDTVVRALQWMQAFNLNHLPVVDGVKYK
                     GLITEYDLKKIEDKEKSLSECELEYQNVYIFETQYIYDALEFVTVKGLNIVPVVSKEG
                     NYRGLLTLVDIIDCFAQNSSVKTPGGIIILNVERKNYSMQEIARITESNGALILSSSV
                     NQLPDSEIFEVTLKVDKLDLSRILAGFYRMDYNVVASYQTTDLPTDIEDRYQAFMTYL
                     NV"
     gene            4672..6075
                     /locus_tag="PKU82_05065"
     CDS             4672..6075
                     /locus_tag="PKU82_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOZ82280.1"
                     /translation="MFKRAFLINLFFAFCFSSFASDSLQVDTNTIYVRQIVVTGNKVT
                     RYSVIQRELAFSQGQTMTVGELNEKITKSRENLLNISLFNFVNINKQLIGNDVYVIID
                     VVERWYIFPVPIFEVVDRNFWEWWRDKNLKKANYGFYLNWENFRGRRENVRLLLRYGY
                     SQRQGFMYSIPSIDKSQNNGLVFTALQTRMREIPYKLENNKLVYFENGSEFLRKEYIA
                     NVRFIHRQGFNKTYTFTGGYQYNIISDTLLSLNRDYFVSGHKYESYPVISFEYKDDAR
                     DIRNYPLKGYFLSLEFTKYGFGISDNDPSITYIIAHFKKFWKLNDSWATAFSFKGKLS
                     GNNDVPYYNQIALGYRGDLVRGYDPYVINGENFALFKSGIKYAIVQPKSFNIPVGLPQ
                     SFTRIPYALYANLFFDAGYVRDKRFYQNNPLSNKWLYSFGAGLDFVTYYDLVLRGEFA
                     FTGYGKSGIFLHLTAPI"
     gene            6088..6969
                     /locus_tag="PKU82_05070"
     CDS             6088..6969
                     /locus_tag="PKU82_05070"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOZ82281.1"
                     /translation="MTIAVYARNVNEKVLAALDVVWTQLHQNDVDVLLYGTLYRHLKK
                     SGKEMQNTHVFSTSAEIAGKADFLFSIGGDGTILDTIKLVKDSGIPVLGINTGRLGFL
                     SIVLPEAITEAINSILKGHYSLDKRAMLRLETDKEIFGDVPYALNELAIHKKDSSSMI
                     IIHAFLNGEYLNSYWADGLLIATPTGSTGYSLSCGGPVISPGSGNFVITPIAPHNLNV
                     RPIVVSDKNVVSLEVEGRNPYFMASLDSRSVTIDSSVQLAVRREDFSFNLVRFDNDNF
                     LQTLRNKLNWGLDARNW"
     gene            7036..7923
                     /locus_tag="PKU82_05075"
     CDS             7036..7923
                     /locus_tag="PKU82_05075"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOZ82282.1"
                     /translation="MNDKYKKLLLVGLILIPFLGSAQIAKKRWKAYRIEYIAGLGATN
                     FLGELGGANRVGSHAFRDLEFSMTRPAAMIGYRYKLSPTFAWCSKFSYGIVSGDDALT
                     KEHFRNNRNLSFKSNIFELSTNIEISFLQEQVGHRYKLRGVRGLRNVEISAYGFLGIG
                     VFHFNPKAKLDGQWYQLQPLGTEGQGQVASRSKYKRTQLCVPLGLGAKYAIDRQISVG
                     LELGLRYTFTDYIDDVSKSYYYDRGGSLVEILADRSHDDNSHVGEQRGDPRWNDAYVF
                     AMFNICYKIRTGRVNYPMF"
     gene            7970..8818
                     /locus_tag="PKU82_05080"
     CDS             7970..8818
                     /locus_tag="PKU82_05080"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOZ82283.1"
                     /translation="MRKILILFFTIVVLQPQKISAQSSELGLLLGVASYKGEINTHLF
                     NPNAFNPAAGLYYKRCLNSFWSFRLGLTYGQITGSDAIADEDFNQYRNLSFRSDIWDA
                     SALFEFNFFPFQTASEHTSKVAPYLVAGITFFHHNPQGFINGSWYDLQPLGTEGQGSG
                     LPNTTGKYKRVQFAFPMGGGIKFKLSNRFSLSVEAIARRTYTDYLDDVSTVYPDQAEL
                     RNTNGVLAAELSDRTIFAGTDINAKRQRGNASDKDWYMMGGFTINWTLSKKYTDKCKP
                     FRTKLR"
     gene            8889..12254
                     /gene="mfd"
                     /locus_tag="PKU82_05085"
     CDS             8889..12254
                     /gene="mfd"
                     /locus_tag="PKU82_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOZ82284.1"
                     /translation="MDLLKDLKEKYIAHPAVQELFLQISKQASKTHLQNTAGSASSFI
                     ATATFEHEPAPMLFILPDREEAAYFLNDLENLNPEQKVFFFPSSYRKPFKLNAHENAS
                     IQMRTEVLSHINKHSQSFIVVSYAEAVAENVVTRHALEKNTIEARVNEQLSIDFVNDF
                     LTENNFQRTDFVFEPGQFAIRGGIVDVYSYAYELPFRLEFDGNKIESIRSFDPVTQLS
                     EKNLHFVTIIPNIQTEMQQNVTESFFKFLPENTLIWTRDLSYSLNYLDDNLTVLKNQT
                     SENDEEEKSDNPRIVYDSIEEIKSVLSTFRQVEFGLKSFYENVVGIKFNIQPQPQFNK
                     NFNLLAGHLKENSDKHFKSYIFSDSAKQVERIYAIFDDLSFKKEVQRDDIQFSPVYTS
                     LYKGFTDLDLKVSVFTDHQIFDRYHRFKLRKNYSRNEAISIKELYSLKPGDYVTHIDH
                     GVGRFGGLEKLNIEGRQQEAIRLVYKDNDILYVSIHSLHRIARYTGKEGTAPSLNKLG
                     STSWASLKQKTKKKVKDIARDLIALYAKRKAQEGFAYTPDNYLQTELEASFIYEDTPD
                     QIKATRDVKKDMESASPMDRLICGDVGFGKTEIAIRAAFKAVNDSKQVAILVPTTILA
                     MQHYRTISERLKDFPCTIDYINRFKSTAQQNETLRKLEVGKIDIIIGTHRLLSKDIKF
                     SNLGLLIIDEEQKFGVASKEKIKSLKVNVDTLTLTATPIPRTLQFSLMGARDLSIINT
                     PPPNRYPVTTELHTFNDKVIREAIMYEVSRGGQVFFVHNRVQDIHDISDMIRKLCPDV
                     KIVVGHGQMDGSQLEEVLMTFIEGDADVLVATTIIESGIDISNANTIIINQAQNFGLS
                     DLHQMRGRVGRSNKKAFCYLLAPPLTVLTPEARQRLQAIEEYSDLGMGFQVALRDLDI
                     RGAGNLLGGEQSGFISEIGFEMYQKILDEALIELRESDFKELFPADDNKPIVADCQIE
                     TDLEILIPTQYVENIVERLNLYKELDDIETEEKLNAFESQLRDRFGPVPKQVMELFET
                     IKLRWKAKSLGFEKLILKNGQLKGYLVSNPDSRLYKSDLFMQIINFVKSNPSRCRMKE
                     DKSKLSLTFYKIETVNEANAVFRQMMKPSVATA"
     gene            12285..12356
                     /locus_tag="PKU82_05090"
     tRNA            12285..12356
                     /locus_tag="PKU82_05090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12318..12320,aa:Arg,seq:ccg)
     gene            complement(12433..19155)
                     /locus_tag="PKU82_05095"
     CDS             complement(12433..19155)
                     /locus_tag="PKU82_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOZ82285.1"
                     /translation="MKSHHYNFPIAPFNEDMLLIDFFDIQASKTPDQIAVYFHEEKLT
                     YAQLEKQAGILAQFLINKGVKPGDLVGLCIERSCNLIVGVLGILKAGAGYVPLDPEYP
                     QERLQYMIETAQMPLLITNKNLSEKIPASKAEKILIEEIFEKHSSPLTQNITHSPTDI
                     AYVLFTSGSTGMPKGVAMPHRPLVNLITWQLKNTKVLGSTKTLQFAPISFDVSFQEIF
                     STLSCGGTLVMIEDEMRLNAVNLLNFVAAEKINRLFLPFIALQHLCEVAENFEGDLSC
                     LQEVITAGEQLQSTQFIKNFFKKNKNCTLWNHYGPTEGHVVTAYQLSSNPDAWAALPS
                     IGKPIDNAEIYILNEEGNVVADNEDGELYIGGVSVAKGYLNRADLTQERFIENPYSNS
                     QGKYFYKTGDLARYMPDGNIEYLGRIDGQVKVRGYRIELGEIELTLDKHATVKQAVVI
                     AREDEPGDKRLVAYCLPHDNKKILAGELRQFLQDKLPEYMMPSAFVQIEEFPRTPSGK
                     IDRRSLPAPSNKRPDIGTVFVAAETDLQKTFVKTWSKLLKVAEIGINDNFFDLGGNSL
                     LALKFIAELRQEHNLDLPVVKMYQHPDIFSLTKFLTNGSATKSFYENAQDRFSSNIKG
                     KIETVEDGIAVIGMAGRFPGANNVDDFWKNIVEGKETVSFFKKQELDNDVPEEDKNDE
                     NYVAVRGIIEDADKFDAPFFNMNPRLAEVTDPQQRIFLQVAWEAIENAGYAPDKYDGL
                     IGVFAGMGNNTYFPNNVFHNKEAINRVGSFQTMTANEKDYIATRIAYEFNTKGPAVSV
                     HTACSTSLTAVTLAYESLLNHECDMALAGGVAITVPVNSGHIYNEGGMFSKDGHTRAF
                     DAGANGTVFSDGAGAVILKRYKDAIKDGDEIYAVIRGAALNNDGSEKASFTAPSVEGQ
                     AAVIAMAQAKAGVSPDSISYIETHGTATPLGDPIEIEALTQAFRSKTDKKQFCAVGSV
                     KTNIGHLTPAAGVAGLIKTCLALKNKILPASLNYSKANAAIDFKSSPFYVNSTLKKWT
                     TENGEPLRAGISSFGVGGTNAHVVVEEAPQKAESGIARKKHLFLLSGRTKDVLDSQTK
                     NLQSFLEKNPDANLADIAYTLQTGRKYFNHRRIISGGDVQEIVSAIQQANPKKTAARE
                     LTTGNPEIIFMFPGQGSQYVNMGKNLYDDEIVFKDAVDTCCNILQPHLGIDLRTILYP
                     EDAEKEKAEKLLKETIYTQPSLFTIGYALAKLWMSWGIQPKGFIGHSIGEFAGAHFAG
                     VFSLEDALMLVANRGRMMQELPHGSMLSVRKAAAEIEKEIDSSCSIAAINGPALCVVA
                     GPAEKIEALQKSLESREITAKLLVTSHAFHSPMMEPMLKPFLEKVKSVKLNKPAIPFV
                     STATSKWITDAEATNPEYWMNHVRATVRFAEGIQTLWGDKPQRVLLECGPRNTAATLA
                     KQQAKEPAKQTAISSLSDSADNYAEWEQILFALGQLWMSGVAIDWQNFYALEKRNRIA
                     LPTYPFEKKRYWLDPINVIQNITQENIQPSVSGFEFPIITQPSVNTTIETTNNTTMNR
                     KDLITAELKTILEESSGMELGNDSSATFLDLGLDSLFLTQAALTISKKYGVKVTFRQL
                     NEEFSSLQSLSAHLDANLPADAMPAAKLAPQQAPQQTNIVMQQPAMQTTGMNPMQMMM
                     MQQMQMMQMMMQQMNQPQQQVAPSVQAHIEKAVEIKSHTKKEDVSENEKAELAKPFGA
                     IARIEKGTSNDISSEQEKWLKQFIAAYTEKTKASKKYCQDYRSVLADPRVVTGFKPAL
                     KEIIYQVVVNKSKGVNFHDIDGNEYVDVLNGFGSNFFGWGNDMLKNAWKKQLDDGVEL
                     GPQTPLAGECAQLICELTGYDRAGFCNTGSEAVLGAMRIARTVTGRNLIICFNGSYHG
                     INDEVIVRGTKKLKSFPAAAGIMPESVQNMLVLDYGTEESLQIIRERMDEIAAVMIEP
                     IQSRRADFHPKEFIHEVRKLTKENNVLMIFDEVITGFRLRLGGAQEYYGVDADLSTYG
                     KVIGGNMPVGAIAGKKQYMDALDGGFWQYGDNSIPEVGVTYFAGTFVRHPLAMAAMKE
                     VLLHLKKEGKPLYEKLNAKTQRLVDEVNAHSKKLGSPFKLVTFGSLFKGKWETEPHYT
                     ELLFAMMRYKGVHIMDGFPCFLTLAFTDADVDFVVSKFKESLSELCEIGFIPSDKNNE
                     QSKINSNGHLQNGNGQHPPIPGARLGKDANGKDAWFIADPERPGKFLMVK"
BASE COUNT         5855 a         3484 c         3684 g         6266 t
ORIGIN      
        1 gagaagccgg tttttatttc ccgtcatgga ttttttcatg gcctatgatt ttgatagtta
       61 tagggatatt tctgggtgta aaacatgcat tccgaaatat ggcatggatg gtattagtac
      121 ttattggcgg attgtttttg atacgaaaca gtttcccgat ttttgaatat agaaatttta
      181 tctggcctgt tgttataatt ctactggggt tgtttattat tcttaaacct tctaatcgtt
      241 ttcgcagagc ttgcaggcga ggcaggtttc acccaagaca tccgcaaagg caatttggaa
      301 actataccgg tacgccatct tcaggtgaag attttttgga agcagtcgca gttttcggaa
      361 gtataaaaaa aaacatcata agtaaggact ttaaaggtgg tgaagtgtcg gctgtgtttg
      421 gtggtgcaga aattaatttt atgcaagccg atatacaggg tagagtagag ctggaggtga
      481 atcaggtttt tggaggaaca aaacttattg ttccagcaca ttgggagatt aagtcagaaa
      541 ttgctgcagt gttaggaagt gttgaagaca aacgtccatt gcgtagtgca tcgctaacag
      601 atgaaaagaa agtactgata ttacgaggta ctactgtatt tggaggaata gacattaata
      661 gttattaaat tttaaattca ttttttaatc aaactaaatt gaaatgtatt ttatagctaa
      721 agtaatttat cagatagtaa tcaacggaag taaaaataat cagtttgatg agcaactttt
      781 aatcattaaa gcacaaactg ttgccgatgc ttatttaaaa gcacgtgcaa ttggcgcaga
      841 gaagcaacat gagtttatta atgatagaaa aaaccctgtt atgtggaaaa tgattgatgt
      901 tgcagatatt atacctgttg aatcacccga gcatggttct gttgtatatt cgcaggttat
      961 tgaagaagac tctgagtcgt ttattgatta tattaaacgg aaagcactga cacttcaaac
     1021 gcaatcactt ttgtttgcct gataaataat gattattgaa aaaataaaaa attcaattga
     1081 ccagattgtt tggtggctat gctggtattt ttttgttgca gcattggttc accaatttgg
     1141 atttgactgg tgcctgtctt ttgcagacag cactgtaatg ttactattat tgttgcttac
     1201 ttcgtgcagc acctattttg catttaataa gtttcaaaaa acaggaaatc gtttttttgt
     1261 gatattatgg aatattgctt tatcgtggat ttctgttttt atattaggct tggtgctaag
     1321 gtatatttat gttgatgatt taaaatattt gacttttctc gattcatcga tggtggttcg
     1381 gtttacatgg ttttttataa tgaatctttt agtaactgtc atctatggtt tgcaagtaaa
     1441 tattttggag cagaaaaaat tagaagcaca taataatgaa aatgccaacc tgatgaaaga
     1501 tgcagaatta tccggattac gattacaact tcaaccacat tttttattca acagcctgaa
     1561 ttctattaat gcattaatag gaacagaacc tgcaaaggca agaagtatgg tgttacagct
     1621 ttcagaattt ttacgcggca ctttacaaag agataataag aaattgataa gccttcagga
     1681 ggaaatcagc catctgaaga tttatcttga aattgaaaaa gtgcggtttg gtcaccgatt
     1741 gattacgcaa attgaaattc ctgaaaactg tttgaatatg atggtgccac cgcttatttt
     1801 acaacctatt gtagagaatg ctattaaatt cggactctat gacaccacag gtcaggtcac
     1861 aatttcatta aaggtttcct gcatggaaag tcatttaagg atattcgttg ccaacccttt
     1921 tgattctgac actgctgcat cgcagaaggg caccggcttt ggccttgacg cagtgcaaag
     1981 gcgattgtat ctgctttatg cacgtaacga tttacttaaa acagaaaaag aaaatagtat
     2041 tttcattaca caaatagaca ttcctcagat atgattaaag caattgtgat agatgatgag
     2101 cctttagcaa gaagtcttgt gttggagttt cttaaagcgt ttccaaacat tgaagtggta
     2161 caggaatgta atgatggctt tgaagggttg aaagccattc agcagtttca acctcaactt
     2221 atttttctgg atatacaaat gccaaagatt aatggttttg agatgcttga gttgattgaa
     2281 aacccgcccg gtgtaatttt tacaactgca tttgacaatt atgcaattaa agcttttgaa
     2341 gcgcatgcca tagactacct tttgaaacca ttcagcgaag aacgatttaa caaggctgtt
     2401 gctaaatttc ttgcaaccaa taataaagca cctgcaatgg atttagtaag tgcggaaagc
     2461 aatattctgc ctgatgaaaa gaatagaatt gttgtaaaag atggaagtaa aattacgatc
     2521 attcctgttg atgatgtaat ttatattgaa gcttatgatg attatgtgaa agttcataca
     2581 caaaatggtt ttaagttgaa gaagaaaacc atgtcgtatt atgaaaacac tttagataat
     2641 tctaaatttt tgcgtgtgca cagaagttat atcgtaaagc ttagtatgat tacccgtatt
     2701 gagcagtatg aaaaaacatc acacatagca ttacttaaaa acggacagaa agttccattg
     2761 agcaaacaag gttatgcaag attaaaatcg gttttaggta tataatttaa aacccaaaag
     2821 cttttttgtc tttattaaag ctaatagtca ttcttgtttt acaggctttt tctttttgtg
     2881 cattagcatc agcaggattt aatttttcca gaaagatatt agtgctgtaa gaataatttt
     2941 tcttgcttaa tttaaccgga aggtgtgtgt tgacttcaaa tttgtacgcg acagcgtcag
     3001 gttttataat agtgatcagg tagtgattgt ttagttttaa tttaagtctg ttctcttttg
     3061 ttgtattcaa gcggtttaca tttccggatt catcagcaat tacaacaaca attgaatcat
     3121 ataaattatc ttcagcagtt atgttggttt ttaatttcag gcattgcgcc tgacaattta
     3181 ctgcagagaa acaaatgata tataacatac atattctaat cattcacgtg cagactttat
     3241 ttgatgttta tacatctgta ccgtattttg taaaccaaag tacaatgcat cactaatcaa
     3301 agcatgaccg attgatactt caataacctg cggaatattt tttataaaat aattaaggtt
     3361 gttcagattt aggtcgtgcc ctgcatttac acccatgttt aaagttgaag catgtgatgc
     3421 tgctttttga tagtcggcaa ttgcctgctg agccgatttt tcatatcctg atgcataacg
     3481 ttctgtataa agttcaattc tgtctgtgcc tgttgttgcc gctgcatcta tttgtttcag
     3541 gtcagtatcc ataaataacg aaacgcgcag gcatttttgt ttcaagcgtt caattacatt
     3601 tttcaaaaat gcttgttcac gaatagcatt ccagccggca ttggaagtca gcacatcagg
     3661 agaatcgggt accaaggtaa cctgatgtgg ctttaccgac tcaaccaatt tcagaaaacc
     3721 ttctgatgga tagccttcga tattaaattc tgtggtaaca cattctttca attcataaac
     3781 atctctgaaa cggatatgcc tttcgtcagg acgtggatgc actgttattc cttcggcacc
     3841 aaaacgctct atatctattg ccgccttaac tacatttgga atagttccgc ctcgggcatt
     3901 gcgcagtgtt gcaattttat taatgttcac actaagttta catgctgtca tatactctga
     3961 aaattgatac aaatgtacta tgtttgccgg cagattcgat ttgggaaaaa atattcatga
     4021 acacaaaagc ttatattaaa gattttattc ctccgttaaa gagttccgac actgttgtca
     4081 gagcattgca atggatgcag gcatttaatt taaatcacct gcctgtggtt gatggggtaa
     4141 aatataaagg cctcataacc gaatacgatc ttaaaaaaat tgaagacaaa gaaaagtcgc
     4201 tttcagaatg tgaattggaa tatcagaatg tttacatttt tgaaacgcaa tatatttatg
     4261 atgcattgga gtttgtaaca gtaaaaggtc tgaatatagt tcctgttgtc agtaaagaag
     4321 gaaattatcg tgggcttctg accttagttg atattattga ttgttttgca caaaacagct
     4381 ctgttaaaac acctggtgga atcattattc ttaatgttga aagaaaaaat tattctatgc
     4441 aggaaattgc acggataact gaatcgaacg gagcattaat tttaagttca tcagtaaatc
     4501 aacttccgga cagtgaaatt tttgaggtta ctttaaaggt tgataaactg gatctttcgc
     4561 gaatacttgc aggattttat cggatggact ataatgtggt ggcatcttac caaactacag
     4621 acttgcccac tgatattgaa gaccgttatc aggcatttat gacatacctt aatgtttaaa
     4681 agagcatttt taataaatct gttttttgca ttttgtttta gcagttttgc ttccgattct
     4741 ttacaggttg ataccaatac aatttatgta aggcagattg ttgtcacagg aaataaagta
     4801 acacgatatt ctgttattca gcgtgagttg gcattcagtc aggggcagac catgactgtt
     4861 ggtgaattaa atgaaaaaat cacaaagagc agagagaact tattgaatat ttcgcttttt
     4921 aattttgtaa atatcaacaa gcagctaatc ggaaacgatg tttatgtgat aattgatgtt
     4981 gttgaacgtt ggtatatttt tcctgtacct attttcgaag ttgttgaccg taatttctgg
     5041 gaatggtggc gcgataaaaa tctgaagaaa gccaattatg gtttttattt aaactgggaa
     5101 aatttcagag gcaggaggga gaatgtaagg ctgttgcttc gttatggtta ttcgcaaagg
     5161 cagggattta tgtacagcat tccaagcatt gataaaagtc agaataatgg gttagtgttt
     5221 actgctttgc aaacaagaat gcgcgagatt ccctataagt tagaaaataa caagttggtt
     5281 tattttgaaa acggaagtga atttttaaga aaagaataca ttgcaaacgt aagatttatt
     5341 caccggcagg gatttaataa gacttatact tttaccggag gttatcagta caacattatc
     5401 tccgatacat tgctgtcgct caacagagat tattttgtta gtggacataa gtatgaaagt
     5461 tatcctgtaa tttcttttga gtataaagat gatgcccgcg atatcagaaa ttatccttta
     5521 aaaggttact ttttaagtct tgagtttaca aaatacggtt ttggcatcag cgataacgat
     5581 ccttcgataa cttatataat agcacatttt aaaaaattct ggaaactgaa cgattcatgg
     5641 gctactgcat tttcttttaa aggcaaatta tcaggaaaca atgatgttcc ttattataat
     5701 cagatagctt tgggctacag aggcgattta gtcagaggtt acgatcctta tgttatcaat
     5761 ggtgaaaatt tcgccctttt caaaagcgga attaaatatg ctattgttca acctaaatca
     5821 tttaacattc ctgtgggatt acctcagagt ttcactcgga taccttatgc attgtatgcc
     5881 aatttatttt ttgatgctgg ttatgtccgt gacaaacgct tctatcaaaa caacccttta
     5941 tcaaataagt ggctctatag ctttggcgca ggacttgact ttgtaactta ctacgatttg
     6001 gtgcttcgtg gcgaatttgc atttacagga tatggtaaat ccggaatttt tctgcacctt
     6061 acagcgccaa tttaataact ttgctcgatg acaattgccg tatatgccag aaatgtaaat
     6121 gaaaaagtgc ttgcagcact cgatgttgta tggacacaat tacatcagaa tgatgttgac
     6181 gttttgttgt atggaacatt gtatcgtcac ctgaaaaagt cggggaagga aatgcaaaac
     6241 acacatgttt tcagcacctc agcagagata gcaggtaaag ccgattttct tttcagcata
     6301 ggtggtgatg gcaccattct cgacaccatt aaattggtaa aagactcagg cattcctgtt
     6361 cttggcatca ataccggcag acttggcttt ttatctattg ttttgcccga ggctataacc
     6421 gaagcaataa acagtattct caaagggcat tattcactcg ataaaagagc aatgcttagg
     6481 ttagaaaccg ataaagaaat ttttggtgat gtaccctatg ctttaaacga acttgccatt
     6541 cataagaagg atagttcatc catgataatt atccatgctt ttttaaatgg agaatatctg
     6601 aactcttatt gggcagatgg acttctgata gccacaccca cagggtcaac aggttattct
     6661 ttaagttgcg gaggacctgt tatttcacca ggatcaggaa attttgtgat tacacccatt
     6721 gccccgcata accttaatgt gcgtcctatc gtagtatcgg ataaaaatgt agtttctttg
     6781 gaagttgaag gtcgaaaccc atattttatg gcatcattgg actccaggtc agtaaccata
     6841 gattcatccg tgcaactggc tgtcagacgg gaggatttca gtttcaatct ggtaaggttc
     6901 gataacgata attttcttca aaccctaaga aataagctaa attggggcct tgatgcccgt
     6961 aattggtaag aatatattat aattgtacct gcaaatattt gaaatgttta tttttgtcaa
     7021 taaccacccc ggcttatgaa cgataaatac aagaaattac tgcttgttgg cttaatttta
     7081 attccctttt taggtagtgc acagattgct aaaaaacgct ggaaggcgta ccgtattgag
     7141 tatattgcag gactcggagc aaccaatttt ttaggtgaac ttggaggcgc aaacagagta
     7201 ggctcacatg ctttccgcga tttggaattt tcgatgacgc gccctgccgc tatgattggc
     7261 tatcgctaca aactttcacc aacttttgca tggtgctcta agttttcgta tggaatagtt
     7321 tcaggtgatg atgctttgac aaaagagcat ttcagaaata accgtaacct cagtttcaaa
     7381 tcaaacatct ttgaattaag caccaatatt gaaatttctt ttcttcaaga acaagttggt
     7441 caccgctata aacttcgtgg cgttagaggt ttgcgaaatg ttgaaatctc tgcctatgga
     7501 tttttaggca ttggcgtatt tcactttaat ccaaaagcta aacttgatgg gcagtggtat
     7561 cagcttcagc ctttgggaac agaaggtcag ggacaagttg catctagaag taaatataaa
     7621 cgtacacaat tatgtgttcc tttaggactt ggtgcaaaat atgcaattga ccgtcagata
     7681 agcgtagggc ttgaattagg tttacgctac acgtttactg attatattga tgatgtaagt
     7741 aaaagctatt attacgacag aggcggctca ttagttgaaa tccttgcaga ccgcagccat
     7801 gatgataact cacatgtagg tgagcagcgt ggcgacccaa gatggaatga tgcctatgtt
     7861 tttgcaatgt ttaatatttg ttataaaata agaacgggta gagtaaatta tcctatgttc
     7921 tgatgtaatt ttcagaataa ttttccgttt ttattgctgt ttttcagcca tgcgcaaaat
     7981 tttaatctta ttttttacaa tcgtggttct tcaaccacaa aaaatatcag cacaaagtag
     8041 tgagttagga ttactgcttg gtgtagcttc ttataaaggc gaaataaata cccatctttt
     8101 taatccaaat gcatttaatc ctgcagcagg tttatattat aaacgttgtc tcaatagttt
     8161 ttggtctttt cgtttaggac ttacttatgg gcagataaca ggtagcgatg ccattgcaga
     8221 cgaagatttt aatcaatatc gcaacctatc attcagaagt gatatttggg atgccagcgc
     8281 tttgtttgag tttaattttt ttccttttca gacagcttcg gaacatacct ctaaagttgc
     8341 tccatatctt gttgcaggaa taaccttctt tcatcacaac cctcaaggtt ttattaatgg
     8401 atcatggtac gatttgcaac ctctcggcac agaagggcag ggtagcggac ttccaaatac
     8461 aacaggaaaa tataagcgtg tgcagtttgc ttttcctatg ggtggcggaa taaaatttaa
     8521 attatcaaac aggttctctt tatctgtaga agctattgca cgaagaacct ataccgatta
     8581 cttagatgat gtgagtacgg tttatcctga tcaggcagag ttaagaaata ccaatggtgt
     8641 acttgcagca gaactctccg acagaacaat ttttgcaggc acagatatta atgcaaaacg
     8701 tcaaagagga aatgcctcag ataaagactg gtacatgatg ggtggcttca ccatcaactg
     8761 gacactttca aagaaatata ccgataagtg taagccattc cgaactaaat tgagataaaa
     8821 tttttaagcg ttcaatttaa aattaagctc aaacttttag ttaattttgc attccgtttt
     8881 ttttacaaat ggatttactt aaagatttaa aagaaaaata tatagcacac cctgctgtac
     8941 aggaactctt tttgcaaata agtaagcaag cttcaaaaac acatcttcaa aatactgccg
     9001 gttctgcctc atcatttatt gcaactgcaa catttgagca tgaacctgct cccatgcttt
     9061 ttattttacc cgacagagaa gaggctgctt attttcttaa cgatttagaa aaccttaacc
     9121 ctgagcagaa agtttttttc tttccttcat cctacagaaa accttttaaa ctcaatgcac
     9181 atgaaaatgc atctattcaa atgcgtacgg aggtgcttag tcacattaat aaacacagtc
     9241 agtcatttat tgttgtatcg tatgccgaag ctgttgcaga aaatgttgta acacgacatg
     9301 cactcgaaaa aaatactatt gaagcgcgtg tgaatgagca actgagcatt gattttgtaa
     9361 atgattttct gacagagaat aattttcaaa gaactgattt tgtttttgaa cccgggcagt
     9421 ttgccatcag aggcggtatt gttgatgttt attcttatgc ctatgaactg cctttcagat
     9481 tagagtttga tggaaataaa attgaatcta ttcgctcatt cgatccggtg acacaattgt
     9541 ctgaaaaaaa tctgcacttt gttaccatca ttccaaacat tcagacagaa atgcagcaga
     9601 atgtgacaga atcgtttttt aaatttcttc ctgaaaatac tttaatctgg acacgcgatc
     9661 tttcttattc attaaactat cttgatgata atttaacagt gcttaaaaat cagacttctg
     9721 agaatgatga ggaagaaaag agtgataatc caagaattgt ttatgattct atcgaggaga
     9781 ttaaatctgt tctgtcaact ttcagacagg tagagtttgg tttgaaatct ttttatgaaa
     9841 atgtagtcgg aataaaattc aatatacagc cacaaccaca gttcaataaa aacttcaatt
     9901 tacttgccgg tcatttaaaa gaaaatagtg ataagcattt taagtcatac attttttctg
     9961 attctgccaa acaggttgaa cgcatctatg ccatttttga tgatttaagt tttaaaaaag
    10021 aagttcagcg tgacgatata caatttagcc ctgtttatac ttcattgtac aaaggtttta
    10081 ctgatttaga tcttaaagtt tctgtattca cggatcatca gatttttgat cgctatcatc
    10141 gttttaaact acgtaagaat tacagccgaa acgaagccat ttccattaaa gaattgtaca
    10201 gcctcaagcc tggtgattat gtaacacata ttgatcatgg tgtaggtcgt tttggtggat
    10261 tggaaaaact aaatatagaa ggtcgtcagc aagaggcaat cagattggtg tataaagaca
    10321 acgatattct ttatgtaagt attcacagcc tgcatcgcat agcccgctac acaggtaaag
    10381 aaggcactgc accatcgctc aataagttgg gtagcacatc atgggcatcg ctcaagcaaa
    10441 aaacaaaaaa gaaggtaaaa gatattgcgc gcgatctgat agcactatat gccaagcgaa
    10501 aagcacagga aggctttgca tacactcccg ataattatct gcaaacagaa ttagaggcaa
    10561 gttttatcta tgaagatacg cccgatcaga taaaagcaac acgtgatgta aaaaaggata
    10621 tggaaagcgc atcgccaatg gacagattaa tttgtggtga tgttggattt ggaaaaacag
    10681 agatagccat tcgtgcagct tttaaagccg taaacgatag taaacaggtt gccattttag
    10741 tgcctactac catattggca atgcagcatt acagaaccat tagcgaaagg ttgaaagatt
    10801 ttccctgtac tattgattac atcaaccgtt ttaagtcaac tgcacaacaa aatgaaacac
    10861 tacgtaaact cgaagtaggt aagattgaca tcatcattgg cacgcacagg ctgctgagca
    10921 aggacataaa attcagtaat cttgggttgt tgattattga cgaagagcag aaatttggtg
    10981 ttgcaagtaa agagaaaatt aaatctctta aagtaaatgt tgacacactg actttgacag
    11041 caacacctat tccaagaaca cttcagtttt cattaatggg tgctcgtgat ttatcaatca
    11101 taaacacacc gccaccaaac cgctatcctg taacaacaga gttgcataca tttaatgaca
    11161 aagttattcg tgaggcaata atgtatgaag tatcgcgtgg aggtcaggtg ttttttgtgc
    11221 acaatcgtgt tcaggatatt cacgatattt ctgatatgat aagaaaactt tgtcctgatg
    11281 taaaaattgt tgtagggcac ggacagatgg atggcagtca gcttgaagaa gtgcttatga
    11341 cttttattga aggtgatgca gatgttttgg tggcaacaac catcatcgaa tccggaattg
    11401 atatcagcaa tgcaaacaca attatcatca atcaggcaca gaattttgga ttgagcgatt
    11461 tgcatcagat gcgtggcagg gtaggacgaa gtaataaaaa agctttttgt tatctgcttg
    11521 caccaccact cactgtgtta acaccagagg cacgtcagcg actgcaggcc atagaagaat
    11581 attccgattt aggaatggga tttcaggtag cgttgcgcga cttagatatt cgtggtgcag
    11641 gaaatttact gggtggcgaa cagagtggtt ttatttctga aataggattt gaaatgtatc
    11701 agaaaatttt agacgaagcg ttgatagaat tgcgcgagtc tgattttaaa gaattatttc
    11761 ctgctgatga taacaaacca attgttgccg attgccagat tgaaactgac cttgaaattt
    11821 tgattcccac acaatatgta gaaaatattg tggagcgatt gaatctttac aaagagttag
    11881 atgatataga aacagaagaa aaactgaatg cttttgaaag tcagcttcgt gacagatttg
    11941 gccctgtacc aaagcaagta atggagttgt ttgaaactat taaactgcga tggaaagcaa
    12001 aatcattagg ttttgaaaaa ctaattttaa agaatgggca gcttaaagga tatttagttt
    12061 caaatcccga ttcacgattg tataagagtg atttatttat gcagataatc aattttgtta
    12121 aaagcaatcc atcgcgctgc cgcatgaaag aagataaaag taaacttagt cttacctttt
    12181 acaagataga aactgtgaat gaagctaatg ctgttttccg gcagatgatg aaaccatcag
    12241 tggcaacggc ataggttgat tattgtacat ttgcagcctt gtatggttct gtagtataat
    12301 ggatagtacg caggtctccg gaacctgaga ttcgagttcg actcccgaca ggaccactta
    12361 gatttcatat tgatattaaa gaagtatcaa tagtaaccgt ttttctattg gattgctaaa
    12421 agcaaaataa tattacttca ccattaaaaa cttacccggt ctttctgggt cggcaataaa
    12481 ccaggcatct tttccattag catcttttcc taaacgagcg cccggtattg gaggatgttg
    12541 tccattgcca ttttgcagat gtccgttaga atttattttc gattgttcgt tgtttttatc
    12601 agaaggaatg aatccgattt cgcaaagctc agataaactt tctttgaatt tagaaacaac
    12661 aaagtcaaca tctgcatctg taaaagcaag tgttaaaaag catggaaagc catccatgat
    12721 gtgaacacct ttatagcgca tcattgcaaa aagtaattca gtgtaatgag gctctgtttc
    12781 ccatttgcct ttaaataaag agccaaaggt taccaactta aatggtgagc ctaatttttt
    12841 actgtgtgca tttacttcgt caaccaatct ttgtgtttta gcattcaact tttcataaag
    12901 cggcttgcct tcttttttaa gatgcaacaa cacttctttc attgctgcca tagcaagcgg
    12961 atgacgtaca aatgttccag caaaataggt tacaccaact tcaggaatag aattatcacc
    13021 atactgccag aatccaccat ctaaagcatc catgtattgt ttcttacctg caatcgcacc
    13081 aacaggcata ttgccgccaa taacttttcc gtaagtactc aggtcggcat caacaccata
    13141 atattcctgc gcaccgccaa gtcttaatct gaatcctgtt atcacctcat caaaaatcat
    13201 cagcacatta ttttctttag ttagttttct aacctcatga ataaattctt taggatgaaa
    13261 atctgctctg cgtgattgaa taggttcaat cattacggca gcaatttcat ccatgcgctc
    13321 gcggataatc tgcaaactct cttctgtgcc ataatctaat acaagcatat tttgtacaga
    13381 ctccggcata attcccgctg ctgccggaaa cgattttagt ttttttgttc cacgaacaat
    13441 cacttcatca ttgatgccat gataagaacc attgaaacaa ataataagat ttcttccagt
    13501 aacggtacgt gctattctca tagcaccgag tacagcctcg ctgcctgtgt tgcaaaaacc
    13561 tgcacggtca tatccggtga gttcacaaat taattgtgca cactcgcctg caagcggtgt
    13621 ctgtggacca agctcaacgc catcatccaa ttgttttttc catgcatttt ttagcatgtc
    13681 gtttccccaa ccaaaaaagt tggagccaaa accattcaac acatctacat attcattgcc
    13741 gtctatatcg tgaaagttta ctcctttaga tttgttaact acaacctgat aaattatttc
    13801 tttcaaagca ggtttaaatc ctgtaaccac acgtgggtcg gcaagcacac tgcgataatc
    13861 ctgacaatat ttttttgaag cttttgtttt ttcagtgtat gctgctataa attgtttcaa
    13921 ccatttttcc tgctcagatg atatgtcatt tgatgtacct ttttcaattc tggcaatagc
    13981 gccaaatggt tttgccaatt cagctttttc gttttcgctt acatcttctt tcttagtgtg
    14041 cgattttatc tcaaccgctt tctctatatg cgcctgaaca gaaggtgcaa cttgttgttg
    14101 tggctgattc atctgttgca tcatcatctg catcatttgc atttgctgca tcatcatcat
    14161 ctgcatgggg ttcatgcctg ttgtttgcat tgccggttgt tgcatgacaa tatttgtttg
    14221 ctgcggtgct tgctgcggtg ccaatttagc tgcaggcatt gcatctgctg gtaggttagc
    14281 atctaaatgc gctgaaagcg attgcagtga tgaaaactct tcgttaagtt gtctgaatgt
    14341 aacttttact ccatattttt tgctgatggt cagggcagcc tgtgtcagaa aaagtgaatc
    14401 caatcctaaa tcaaggaagg ttgcactgct gtcgttgccc aattccatac ctgaagattc
    14461 ttcaagaatt gtttttaact ctgctgtgat aagatctttt ctattcattg ttgtattgtt
    14521 cgttgtttca attgttgtgt ttacagatgg ctgagtaatg attggaaact caaagccgga
    14581 aactgatggt tgaatgtttt cttgtgtaat attttgaatg acattaattg ggtcaagcca
    14641 atatctcttt ttttcaaaag ggtaagtagg tagtgcaatg cgatttcgtt tttcaagtgc
    14701 ataaaaattt tgccagtcaa ttgcaactcc gctcatccac agttgaccta aggcaaagag
    14761 tatttgctcc cattctgcat aattatctgc tgaatcactc agcgatgaaa ttgctgtttg
    14821 tttggcaggt tcttttgcct gctgtttagc taaggttgca gcagtatttc ttggtccaca
    14881 ttccaacaaa acacgttgtg gtttatcacc ccacaaagtt tgaatacctt cagcaaaacg
    14941 tacagtagct cttacgtgat tcatccagta ttccggattg gttgcttctg catctgtaat
    15001 ccattttgag gtggctgttg atacaaatgg tattgcaggt ttattgagtt taacggattt
    15061 tactttctct aaaaaaggtt tcaacatggg ctccatcatt ggcgaatgga atgcatgtga
    15121 tgtcactaat aactttgctg taatttcgcg actttctaat gatttttgaa gtgcttcaat
    15181 tttttcagca gggccggcaa ctacgcaaag tgcagggccg ttaattgcag caatagaaca
    15241 agaagaatca atctcctttt caatttccgc agcagcctta cgaacactta acatacttcc
    15301 atgtggtaat tcctgcatca ttcgtccgcg attggcaaca agcatcagtg catcttctaa
    15361 agaaaatact cctgcaaaat gtgcacctgc aaattctcca atgctgtgac cgataaatcc
    15421 cttgggttga ataccccaac tcatccaaag ttttgctaat gcatagccga ttgtaaacaa
    15481 cgatggctgc gtataaatag tttctttcaa aagcttttct gctttttctt tttcagcatc
    15541 ttcaggataa agaattgtcc gcaagtcaat acctaaatgt ggttgcaaaa tgttacagca
    15601 tgtgtcaacg gcatctttaa aaactatttc atcatcatat aaattttttc ccatgtttac
    15661 atactgtgaa ccttgtccgg gaaacataaa aattatttca ggattgcctg ttgtcagttc
    15721 gcgtgcagct gttttctttg gattagcttg ttgtattgct gatacaatct cttgaacatc
    15781 accacctgaa ataattctgc gatgattaaa atattttcgt cctgtttgta aagtgtaagc
    15841 aatatctgca agatttgcat ccggattttt ctctaaaaac gattgtagat tttttgtttg
    15901 tgaatccaat acatccttag ttcttcctga aagtaaaaac aaatgttttt ttcgggcaat
    15961 gcctgattca gctttttgag gtgcttcttc aactactaca tgagcgttag ttccgccaac
    16021 accaaaagaa ctgatgcctg cacgcaaagg ctcaccattt tctgttgtcc atttttttag
    16081 ggtggaatta acatagaatg gagaagattt aaaatctatt gcagcatttg cttttgaata
    16141 attcagtgaa gcaggtagta ttttattttt tagtgcaaga catgttttaa ttaatcctgc
    16201 aacgcctgct gccggtgtca ggtgtccgat attggttttt actgagccaa cggcacaaaa
    16261 ttgtttttta tcagttttgc ttctaaatgc ctgagtgagt gcttcaattt ctattgggtc
    16321 accaagtggt gtggcagtac catgtgtttc aatatatgaa atggagtcag gcgacacacc
    16381 tgcttttgcc tgtgccattg caattaccgc agcttgtcct tcaacactgg gtgcagtaaa
    16441 acttgctttt tcactgccat cattatttaa ggcagcacca cgaattactg cgtaaatctc
    16501 gtcaccgtct tttatggcat ctttatatct tttcaggata acggcacctg caccatcact
    16561 gaatacagtt ccgttggcac ctgcatcaaa agctcttgta tgtccgtctt tactgaacat
    16621 cccaccttca ttatagatat gtccactgtt tacagggact gtaatagcta cacctccggc
    16681 caatgccata tcacattcat ggtttaacag actttcatag gcaagtgtaa ctgcagtgag
    16741 cgatgttgaa catgcagtat gcacactcac agccggtcct tttgtattaa actcataagc
    16801 tattcttgta gcaatataat ctttttcatt tgcagtcatt gtttgaaatg agcctacacg
    16861 gttaattgct tctttattat gaaaaacatt attcggaaaa taggtgttgt ttcccattcc
    16921 ggcaaaaact cctattagtc catcatattt gtcaggagca taccctgcat tttcaatagc
    16981 ttcccatgcc acctgtaaaa aaatgcgctg ttgcggatct gtaacttctg caagtcgcgg
    17041 attcatatta aaaaacggag catcaaattt gtcggcatcc tcaattatac ctcgaacagc
    17101 cacgtaattt tcatcgtttt tatcttcttc cggcacatca ttatctaatt cctgtttttt
    17161 gaaaaaagat acagtctctt tgccttcaac aatgtttttc cagaaatcat caacattgtt
    17221 tgcaccggga aaacgccctg ccataccaat tacagcaatg ccatcttcta cagtttcaat
    17281 cttgccttta atgtttgaag agaatctgtc ctgtgcattt tcataaaatg attttgttgc
    17341 agacccgtta gttaaaaatt ttgtcagaga aaaaatatca ggatgctgat acattttaac
    17401 aacaggaaga tctaaattat gctcctgcct tagttctgct ataaatttta atgcaagaag
    17461 tgagtttcca ccaagatcaa aaaagttgtc attaatacca atctcagcaa cctttaataa
    17521 cttactccat gtcttgacaa aagttttttg caaatctgtt tctgcagcaa caaatacagt
    17581 gcctatatct ggccttttat tagatggtgc aggtaaactt ctgcggtcaa tttttcctga
    17641 aggtgtgcgt ggaaattctt ctatctgtac aaatgcagat ggcatcatgt attcaggtaa
    17701 tttatcttgc aggaattgac gaagttctcc tgctaaaatt ttcttattgt catgtgggag
    17761 acagtatgca actaagcgtt tatcacccgg ttcatcttct cgtgcaatta caacagcttg
    17821 ttttactgtt gcatgtttat caagagttaa ttcaatttct cccaattcaa ttctgtagcc
    17881 acgcactttc acctgaccat caattcttcc gaggtattct atattgccat cgggcatata
    17941 acgagcaaga tcaccggttt tgtagaaata ttttccttgc gaattgctat atggattttc
    18001 tataaatcgt tcctgtgtta agtcagctcg attaagatag ccttttgcaa cacttacacc
    18061 accaatataa agttcaccat cttcattatc tgcaacaaca tttccttctt cgttcagaat
    18121 ataaatttct gcattatcta taggcttacc aattgaaggc agtgcagccc aagcatcagg
    18181 atttgaagag agctgatatg cagtaacaac atgcccttca gttggtccgt aatgattcca
    18241 caatgtgcaa tttttgttct tcttaaaaaa gtttttgata aactgtgttg actgtaattg
    18301 ttctcctgct gtaataactt cttgcagaca gcttaaatcg ccttcaaaat tttcagctac
    18361 ttcgcataga tgctgcaaag caatgaaagg taaaaacaaa cgattaattt tttcggcagc
    18421 aacaaagttt aaaagattta ctgcattcaa tcgcatttca tcttcaatca tcaccaaggt
    18481 gccaccacaa cttagtgtag aaaaaatttc ctgaaatgaa acatcaaaac ttatgggagc
    18541 aaattgtaat gtttttgttg agcccaaaac cttggtattc ttcaattgcc atgtaatcag
    18601 attaaccaaa ggtctgtgag gcatggcaac acccttaggc ataccggttg atccactggt
    18661 aaaaagtaca taggcaatgt cagtaggaga gtgcgttata ttttgagtga gtggcgaaga
    18721 atgtttttca aaaatctctt cgattaaaat tttttctgcc ttacttgcag gtattttttc
    18781 cgaaagattt ttattggtga tgagcagtgg catttgcgca gtctcaatca tatattgcaa
    18841 tcgctcttga ggatattcag ggtcgagagg aacatatccg gcaccggcct tcagaatccc
    18901 caatacacca acaataagat tacaactgcg ttcaatacaa agaccaacaa gatcacctgg
    18961 ttttacacct ttgttgatca gaaattgtgc aagaattccg gcttgtttct ctagctgtgc
    19021 gtaagttaat ttctcctcat gaaaatatac ggcaatttgg tcaggtgttt tagaggcctg
    19081 aatatcaaaa aaatcaatca gcagcatatc ctcgttaaag ggtgctatag gaaaattgta
    19141 atggtgcgat ttcattaagt tggtggttta aaaactatta tgtgccgccc ttcggtacaa
    19201 ctatattgta cggaagcgaa tataataagt ttcactttat cacgataata taccagatta
    19261 tgtgcagcag gcaatgcttt gatgatgtc
//
LOCUS       DAOOOL010000041        17780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_252_length_17780_cov_85.717969, whole genome shotgun sequence.
ACCESSION   DAOOOL010000041 DAOOOL010000000
VERSION     DAOOOL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_17780_cov_85.717969"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..778
                     /locus_tag="PKU82_05100"
     CDS             248..778
                     /locus_tag="PKU82_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ82286.1"
                     /translation="MSETYKTFPGGLYFVTFTTVGWLDIFTRRVYQDILINSLIYCQQ
                     DKGLQLYCYCVMPSHVHIIAKRKEGILNDLIRDMKAFSSKKLMDAIEENMLESRREWL
                     IKEFKYYGKISPHKQYRQFWQHNNHAFELYSNRIIDQKINYIHNNPVEAGFVDEPHLW
                     RLSSANENSPVKVLSM"
     gene            complement(938..1204)
                     /locus_tag="PKU82_05105"
     CDS             complement(938..1204)
                     /locus_tag="PKU82_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82287.1"
                     /translation="MKKVMIAAAALIIGGTAFANAQTNAKPAATTTHQAAPAATHETA
                     AQKKEVKEEKMEKKEATPAKHETKKPAAHKSHAKKVEKKEESAK"
     gene            complement(1455..1889)
                     /locus_tag="PKU82_05110"
     CDS             complement(1455..1889)
                     /locus_tag="PKU82_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82288.1"
                     /translation="MKRIMLLAGLCLALTATMRAQVVQKNEISDQAQQQLLQLNDEMA
                     TSFQHTDLLKVSEFFDDDATIYMNGNKVTGRKALSDFWNKITARKKMEINVDEMNGTG
                     KFIYETGTLTMNTENNTVTKNFVMVWKRLPNFDYKIVVAGFN"
     gene            complement(1991..3691)
                     /locus_tag="PKU82_05115"
     CDS             complement(1991..3691)
                     /locus_tag="PKU82_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOZ82289.1"
                     /translation="MNKTVTVNIGGIVFHIEENAYDMLGSYLQAIRNHFTISDGRDEI
                     IQDIESRIAEIFQQNLGTARQVVTADDVNQAIGIMGRPEDFGATEEASANNASASSEQ
                     NNQSDHFFRRRLYRDTDDKVIAGVCSGIGHRLGIDAVWMRLLFIISVFFAGSGIGVYI
                     ILAIVMPKAITPTQKLEMRGDPVNISSIRRETESPGSTKQPSAVSGVFDTLGDLIKGL
                     FKFVVYAIMAILAFAGLMMLIGIGLFILAMLGVSGLTIPIYISDQFLSSSQQWWVSLS
                     VLLLIGVPAIWLMTLLIKWIFRIKYNNRFLNVAAGMLFMAGIVIAFFTGLNIARDFAF
                     DGKVRNTVALITPPSPTMYLKLMDDPKYDEEDNSKFFHRSGRNYSITTGSSFEFNSDD
                     VQFTVQKSPSDKFELVRVFSANGTTENLAVESARGIIYNFEQKDSLLMFSNHFPLPKG
                     TKFRNQRVDLVLKVPVGSSIFLDESMEEIIRDIPNVTDTWDGDMGGHTWKMTENGLIC
                     TDFEFQKGDSTDIHNPENVDISIDNNGVRIKGKEAKSGDKDVNINIGKDGVHIKSTDK
                     "
     gene            complement(3688..3999)
                     /locus_tag="PKU82_05120"
     CDS             complement(3688..3999)
                     /locus_tag="PKU82_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOZ82290.1"
                     /translation="MRKGVLEFCILSIISKGEVYPSDIIEKMKEARLIVVEGTLYPLL
                     TRLKNSGLLSYTWIESNAGPPRKYYKLTPLGKEHLKELKKTWDELVDAVSQTYQNSTE
                     E"
     gene            complement(4350..5933)
                     /gene="ftsZ"
                     /locus_tag="PKU82_05125"
     CDS             complement(4350..5933)
                     /gene="ftsZ"
                     /locus_tag="PKU82_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOZ82291.1"
                     /translation="MPKELPSIIKVIGVGGGGSNAVNHMYKLGIKGVDFVVCNTDAQA
                     LEMSPVPRKIQLGPSLTEGRGAGSIPEVGKQSAIENIEELKAILSDNTKMVFITAGMG
                     GGTGTGAAPIIAGVAREMGILTVGIVTVPFAFEGRKRKQQADAGIEELKNSVDTLLII
                     CNDKLREIHGNLKLNTAFGYADNVLATAAKSIAEVISVTLHINVDFADVQTVMRDSGV
                     AIMGAAQSSGEGRAIRAAELALDSPLLNDNNIEGARHILLNITSGKEEITMDEMGDIT
                     DYIQQKAGQTAEIIMGVGTDDSLGDKISVTIIATGFNADKEKTKEEKVKQPEKVVLNI
                     YDETPTSVVNEVSETTVEEEPVMNEPVLITLEQDIPVTSTAFFEKDNTPEEVVADKST
                     MEFEFTGFPNVISQDEEITAPEATLTEEPTVSELPKSQESLPEVPSQESREPNSDIYR
                     RTRERIETMKSSLNSRTLNPQALNEMEKEPAYMRKNVKLQNVLSSGDTNYSRYSLNNE
                     QDKTPGLSKGNKFLHDRPD"
     gene            complement(5989..7281)
                     /gene="ftsA"
                     /locus_tag="PKU82_05130"
     CDS             complement(5989..7281)
                     /gene="ftsA"
                     /locus_tag="PKU82_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOZ82292.1"
                     /translation="MAEQVTGNEVIVGLDIGTTKIAAIVGRRNEFGKIEIVGTGRAES
                     VGVTRGVVANIEKTVGSIKQAVAEAEHKCGGNIKSVHVGIAGQHIKSLQHRGMITRSS
                     TDDEISQRDIDALIDDMYKLAMPPGEEIIHVIPQEFIVDNEQGIKDPIGMSGIRLEAN
                     CHIITGLVTAANNIHKCVTKAGLTPADLILEPLASAEAVLTEEEKEAGIVLVDIGGGT
                     TDVAIFQDNIIRHTAVIPFGGNVITEDIKEGCHIIRNQAELLKIKFGSALSSQMKDNE
                     IVSIPGLRGREPKEISVKNLASIIQARVEEIIEQVYYEIKTSGFEKKLIAGIVVTGGG
                     AQLRHITQLMEYITGMDTRLGYPNEHLAKSAEETKSPIYATGVGLVIKGFQDLDKHRK
                     SGQKNTSEPRVQHKGSWFSGIFDSTKKIFTDDSDDKII"
     gene            complement(7294..8139)
                     /locus_tag="PKU82_05135"
     CDS             complement(7294..8139)
                     /locus_tag="PKU82_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82293.1"
                     /translation="MKTNFKISDKLRSVLLIAGYVLVAATVLTLMSFATKRNHDLVCK
                     GVVVNIEDENVKGFIDRSDIMEIVRSKGKKIIGNKVTDINTFMLEKIIDANPHVLKAE
                     VYSTIDGWVHIDVQQRTPIVRIINSKGENFYIDNQGKFMPLSEKYSEPVMVASGQIAE
                     GLVQQKLGLDINSLPLPDDSVGKLPMLSQVFLLAEQLKRDSSWDAMFEQIYVDENGEI
                     ELIPRLGNNYILLGNISNLDEKLNLLLNLYRQGFPATGWDQYESINLKFKGQVVCKKK
                     NVKLS"
     gene            complement(8136..9551)
                     /gene="murC"
                     /locus_tag="PKU82_05140"
     CDS             complement(8136..9551)
                     /gene="murC"
                     /locus_tag="PKU82_05140"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOZ82294.1"
                     /translation="MKFENITHVYFIGIGGIGMSALARYFKAIGKQVAGYDKTQTALT
                     EELKGENIDVHFNDDVQAIPLAFMEANRKSNILVVYTPAIPKDHSELNFFSQQEFTVV
                     KRSQVLGVITSMHKTLAVAGTHGKTTTSSILAHILKTANINVAAFLGGIARNYNSNII
                     LPVSEHEVINVVEADEFDRSFLTLFPFAAAITSMDADHLDIYGSGEELKKTYQQFASQ
                     VDEKGLLVLRNGLQLQPVKALTKFYAADSQADYFADKISIRNHRYCFNFHSPDGIIED
                     LSLGLPGRHNVENAVAAIALAMKVGVKEESVRMALQSYKGVKRRFDIRFSDANCTFID
                     DYAHHPEELRAAIMSAHELFEGKKITGIFQPHLFSRTRDFADEFAASLALLDEIILLD
                     IYPARELPLEGITSSWLMNKIKNANKIFLNKTDVLDYINKREPEVLITLGAGDIDTLV
                     TPIQQLLEKKYSVHEFKNHKQ"
     gene            complement(9548..10696)
                     /gene="murG"
                     /locus_tag="PKU82_05145"
     CDS             complement(9548..10696)
                     /gene="murG"
                     /locus_tag="PKU82_05145"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOZ82295.1"
                     /translation="MQQPDIKVIISGGGTGGHIFPALAIANAIKSARPQAEILFVGAK
                     GRMEMEKVPAAGYKIEGLWISGIKRELTIDNLSFPFKVLSSISKAMHILKEFKPNAAV
                     GVGGYASGPLLYAASLKKIPCLIQEQNSYPGITNKILAKRVQKICVAYEGMDKYFPAS
                     KIILTGNPVRENVLMLDGKFDEAMSFFSFNPSQKTLLIVGGSQGARSINKSILAGLEK
                     LKAAGVQLIWQTGKLFYEEAQRAVAEVGYGQARVFDFIARMDFAYAAANLVVSRAGAS
                     TVSELTLTGKPSVMVPLPTAAEDHQTKNIEAMVKRDAAVLVKDTDALQHLADTALNII
                     NDADRLKQLSANAAAMALPHAAQKIADEVIAMATQHAAQNLKHVTAGS"
     gene            complement(10683..11840)
                     /locus_tag="PKU82_05150"
     CDS             complement(10683..11840)
                     /locus_tag="PKU82_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOZ82296.1"
                     /translation="MNWIETYFKGDRIIWIVVILLSLMSLMAVYSSTGTLAYKYQEGN
                     TEYYLFKHGIIVFLGFGIMYFTHLVKYTFYAKVSRIAIFLAIPLLFLTLLTGTNLNEA
                     SRWLTLPVINLSFQTSDLAKIALLLYVARSLSKKQDNIKDFKSAFLPVILPVCIICGL
                     ILPANFSTAAVLFTTCLVLMFFGRINFKYILSLVGIGIAGLGLFLLVASFSGYEGRIG
                     TWKKRIENFSNGNDQGNYQTQQAKIAIASGGVFGKGPGQSTQRNFLPHPYSDFIFAII
                     IEEYGFVGGLVIVVLYLLLFYRAVVIVRKSPFAFATLLAIGCAFSLVFQAMINMAVAV
                     NLFPVTGQPLPMLSMGGTSIWFTSIAVGIILSVSRNIEQEELKTGVVHAAA"
     gene            complement(11858..13201)
                     /gene="murD"
                     /locus_tag="PKU82_05155"
     CDS             complement(11858..13201)
                     /gene="murD"
                     /locus_tag="PKU82_05155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOZ82297.1"
                     /translation="MSKNKRIVILGAAESGVGAAILAQKQGYDVFVSDLSRIADNYKT
                     HLTERNIQFEEGCHTDSLILNADEVVKSPGIPDKAAVIKKIKSANINIVSEIEFASRF
                     TNAKMIAITGTNGKSTTTLLTYHILKKAGVNVGLAGNIGKSFAWQVAEENFDHYVLEL
                     SSFQLDDMYTFKADIAVLLNITPDHLDRYEYSIQNYIDAKFRIIQNQTAEDAFIYCFD
                     DAVVKTEVLKRNPLATCYPFSIRQDEGMSAFVNDNNLYLNIHNNLFTMSIFELALQGK
                     HNLYNSMAAGVAARILDIRKESIRESFADFRNAEHRMEFVNTVHGIDFINDSKATNVN
                     SAWFALESMQKPVVWILGGVDKGNEYEMLNDLVKEKVKAIVCLGKDNKKIHEAFGSIV
                     SDITDTLSAQEAVSVAYQKGKKGDVVLLSPCCASFDLFKNYEDRGRQFKAAVKAL"
     gene            complement(13206..14462)
                     /gene="mraY"
                     /locus_tag="PKU82_05160"
     CDS             complement(13206..14462)
                     /gene="mraY"
                     /locus_tag="PKU82_05160"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOZ82298.1"
                     /translation="MLYYLFEYLDQHTNLPGMGVFKYISFRAALAIMFSLLFSFILGR
                     TIIAYLHKKQVGETIRDLGLDGQVQKQGTPTMGGLIILSAILIPTILFAKLLNVYIIL
                     MLISTVWLGAIGFLDDYIKVFKKDKKGLAGKFKVLGQIGLGLIVGSVLYFNDHVVVRE
                     KAVRSAVTWHHDNTLVDDLQSQEGVLFSTTNIKSTKTTIPFFKNNEFDYASLLGWLGD
                     GYKNLAWLIFIPVVIIIVTAVSNGANITDGIDGLAAGSSGIIGLALAVFAYVSGNTVF
                     ANYLNIMYIPNAGELLVFMSAFVGACIGFLWFNAYPAQVFMGDTGSLALGGIIAVFAI
                     AIRKELMIPVICGVFLIENLSVVMQVSYFKYTKKKFGEGRRIFKMSPLHHHYQKLGYH
                     ESKIVTRFWIVGIMLAVISLVTLKLR"
     gene            complement(14462..15925)
                     /locus_tag="PKU82_05165"
     CDS             complement(14462..15925)
                     /locus_tag="PKU82_05165"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOZ82299.1"
                     /translation="MQLLKEVLYKAGTEEVIGNTNVAVYGICINSRVIKPGEMYVALR
                     GTNVDGHAFILEAIEKGATSVLCEVMPDLIQAGINYIKVKNTYLALSEVCANFYHHPS
                     SSLKLVGITGTNGKTTTATLLYKLFRQLNIRCGLISTVENRVNDTVIPATHTTPDPIK
                     LNQLLSMMVLEECEYCFMEVSSHAVVQNRIGALEFAGGVFTNITHDHLDYHKTFEEYL
                     KAKKKFFDGLPESAFALTNADDKNGMVMVQNTHAAKKTYGLHGIADFRCRIIENNFSG
                     LLLQIDNTEVLCKLIGAFNAYNLLAIYATAVSLGLDKQRVLTALSMLESVNGRFDYVV
                     SGSGITGIVDYAHTPDALNNVLSTINEIRTRNEKVITVVGCGGNRDTAKRPVMAHIAC
                     EMSDKVILTSDNPRNEDPQAIIEQMRAGVPAHLSSKVMAITDRNEAIRVACNLAAAGD
                     IILIAGKGHETYQEIKGVKHPFDDKKILQENFNNLHA"
     gene            complement(15925..>17780)
                     /locus_tag="PKU82_05170"
     CDS             complement(15925..>17780)
                     /locus_tag="PKU82_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOZ82300.1"
                     /translation="MKAESITDKIFNAGIDSLALNLAALFQDKTKDQYKRMLREGRRD
                     GERYLLVKRNVSYAELKKVKTFPIFNKGRYKGGLIVEQKNMRQKPFKELASRTIGFKI
                     GNVQPVGLEGAFDQSLRGESGKRLMQKISGNIWKPLNAEDEVEAKDGCDIYTSIDLNI
                     QDVAQQSLYNQLVKNDADAGCAILMEVATGEIKAIVNLKKADSGYYREDFNYAIGYAT
                     EPGSTFKLVSMMAGIEDGYIDLEDTIDATGGRTSFAPGLIMRDSHEGLNKITAKHAFE
                     ESSNVGVSKLIQKYYRKNQQAFIDRVHKMHFGDKLNIQIAGEATPLIKSTTSKEWSKI
                     SLPYMSIGYETMVTPLHTLTFYNAVANNGRMVKPLFVKEIRYRGHAVKEYKTQVIADS
                     ICSKSTIAKARELLEGVVQNGTGRKLKDCVYAVAGKTGTAQMANNKGGYKSGRVRYQA
                     SFCGYFPADKPAYSCIVVVYNPSTAGYYGGEVALPVFKDIADKVYATNLDMHKGLDVQ
                     IAELPKVKAGLTKATIAACNNVGVDVKTVGTASKWSSVDKREAVAALKPVSIKDNTVP
                     NVEGMGLRDAIYMLETNGLQVKVVGRGAVVKQSLQAGTRIYRGQVITIELS"
BASE COUNT         5161 a         3780 c         3172 g         5667 t
ORIGIN      
        1 atttttttat ccatgtttta cactttttag tgtaaaccta tttcaggaca agacagcatt
       61 gaacaacacc gtacccgcaa agcccctgct tcgtgggcct tgtgtttaga agctgtgctt
      121 cgaacagcaa tgaactgtac ccgcgaagct cctgcttcgt gggccttgtg tatagaagct
      181 gtgctttgaa caccaatgaa caacattata aataaaatta caacatttgt ataaacataa
      241 atatataatg tcagaaacat ataaaacatt tcctggaggg ttatactttg taacttttac
      301 cactgtgggc tggttagata tatttacaag aagagtttat caggatatac taattaatag
      361 tctaatctac tgccaacaag acaaaggctt gcaactgtat tgttattgtg taatgccaag
      421 tcatgttcac attattgcca aaaggaaaga aggaatattg aatgacctga tacgtgatat
      481 gaaagccttt tcgtcaaaaa aacttatgga tgccatagaa gaaaatatgc ttgaaagcag
      541 acgtgagtgg ctaattaaag aatttaaata ttatggtaaa atatcacccc ataaacaata
      601 caggcagttt tggcaacata acaatcatgc atttgaatta tatagcaaca ggataataga
      661 tcaaaaaata aattacatac ataacaaccc ggtggaagcc ggttttgtag atgaaccgca
      721 tttgtggcgg ttaagtagcg ctaatgagaa cagccccgtt aaagtgttga gcatgtaatg
      781 caaagcacag cttttaggta cttaacacca tgtaatgcaa agcacagctt ttatgcattt
      841 atcccacgaa gcaggagctt cgcgggtacg ggggtgtata ttaaaaaaaa agaggctgcc
      901 tcaaaaaatt gagacagcct cacgtttcaa aatcaattta cttagcgctt tcttcttttt
      961 tctctacttt tttagcgtgg ctcttgtgcg cagcaggttt ttttgtttcg tgctttgcag
     1021 gtgtagcttc ttttttctcc atcttctctt ctttcacttc ttttttctgt gccgctgttt
     1081 catgtgttgc ggcaggtgca gcctgatgtg tcgtagtagc tgcaggtttt gcattagttt
     1141 gcgcattggc aaaggcagtg cctcctatta taagggcagc tgcggcaatc attacttttt
     1201 tcattttatt tatttttttg ttttgaaatg atgggtcaaa tgttcaattg ccaacatgat
     1261 gttgatatga aaatttgcac atcttcattt ttgtgataat cagacttcat aatcccgtca
     1321 tccatgaagt taatgtttaa caatcacaag aattgatatt atccaagcta agattaaaac
     1381 aacaagaggt ctgcaaataa aatacagacc tctgttatac aaacacaaat aatcagttac
     1441 tcaaactaat ttttttagtt aaacccggca acaacaattt tataatcgaa gttgggcaaa
     1501 cgtttccaca ccattacaaa gtttttagtt acggtattat tttctgtatt cattgtaagc
     1561 gttccggttt cgtaaatgaa tttgcctgta ccattcattt catcaacatt aatttccatt
     1621 ttttttctgg cagtaatttt attccagaaa tcagacagtg cctttcggcc ggttacctta
     1681 tttccattca tgtagatggt ggcatcatca tcaaagaact cgctcacctt tagtaaatct
     1741 gtatgctgaa atgatgtagc catttcatca ttgagttgca gcaactgttg ttgtgcctga
     1801 tcgcttattt catttttctg aaccacctgt gcgcgcattg tcgcagtgag tgcaaggcat
     1861 agccctgcca ataacataat tcgcttcata atatttgatt taaaatttag ttgatattga
     1921 ttgataaagg aattaacctt tatgtgtagg acgaatacta tcctgaattg ttacagcgtt
     1981 gtagaggaat ttatttatcg gttgacttaa tatgtacacc atcttttccg atgttgatat
     2041 ttacatcttt atcaccactc tttgcctcct tacccttgat gcgcacacca ttgttatcaa
     2101 tactgatgtc cacattttcg ggattatgaa tatcggtgct gtcgcctttc tgaaattcaa
     2161 aatcagtaca tatcaaacca ttctctgtca tcttccaggt atgtccgccc atatcgccat
     2221 cccaggtgtc ggttacgttt ggaatatctc tgataatttc ttccatactt tcatccagaa
     2281 aaatgctgct gccaacagga actttcaaaa ccaaatcaac acgctgatta cgaaacttgg
     2341 ttccctttgg caatggaaaa tgattgctga acatgagtaa agaatctttc tgttcgaagt
     2401 tataaatgat gcctcgtgca ctttcaacgg caagattctc tgttgttccg tttgcagaaa
     2461 acactctgac caattcaaac ttatctgatg gcgatttctg aacagtaaac tgcacatcgt
     2521 ctgaattaaa ctcaaagcta cttcctgtag tgatgctata gttacgacca ctgcgatgaa
     2581 aaaatttact gttatcttct tcatcatatt tagggtcgtc catcaacttt aaatacatag
     2641 ttggcgaagg cggtgttatc aacgcaacag tattacgcac cttaccatcg aaggcaaaat
     2701 cgcgtgcaat atttaatcct gtgaagaagg caataactat acctgccata aacaacatgc
     2761 ctgcagcaac atttaaaaag cggttgttgt attttattct gaaaatccat ttaataagca
     2821 aggtcattaa ccaaatggcc ggaacaccga tgagtagcaa cactgataaa gatacccacc
     2881 actgttgcga gcttgacaga aactgatcgg atatgtagat tggaatagtg agtccgctaa
     2941 caccaagcat tgccaaaata aacaagccaa ttccaatcaa catcataagt cctgcaaatg
     3001 ctaatatggc catgatggca taaacaacaa acttgaaaag tcccttaatt aaatcgccaa
     3061 gagtatcaaa tactcctgat actgcagaag gttgtttggt ggaaccgggc gattctgttt
     3121 cgcgtctgat gcttgaaata tttacagggt cgccacgcat ttcgagtttc tgagttggtg
     3181 taatggcttt gggcataact attgccagaa tgatgtaaac cccaattcca cttcccgcaa
     3241 aaaatacact aatgataaag agcaagcgca tccacactgc gtcaatgccc aaacggtggc
     3301 caatgccgga acaaacacct gcaataactt tatcgtcagt atcgcgatac agcctgcgtc
     3361 tgaagaaatg atcggactga ttgttttgtt cactgcttgc tgatgcattg tttgctgatg
     3421 cttcttctgt tgcaccaaaa tcttcaggtc gtcccataat gccgattgcc tggttcacat
     3481 catcagcagt aacaacctgc cttgctgtgc ctaagttttg ctggaatatt tcagctatac
     3541 ggctttcaat gtcctgaata atttcatcgc gtccatcact gatggtaaaa tggttgcgga
     3601 tagcctgcag ataactgcca agcatgtcat aggcgttttc ttctatatgg aaaactatgc
     3661 ctccgatgtt tactgttact gtcttgttca ttcttcagtt gagttttggt aggtctggct
     3721 gacagcgtca accagttcat cccatgtttt ttttaattct tttaaatgtt cctttcccaa
     3781 tggagttagt ttgtagtact tgcggggagg tcctgcattg ctttcgatcc aggtgtagga
     3841 taacaaacct gaatttttta atcgtgtcag caatggataa agtgttccct ccaccacaat
     3901 gagtctggct tctttcattt tttcgatgat atcgctggga taaacttctc cttttgatat
     3961 gatggataaa atgcagaact ccaaaactcc tttgcgcatt tgcgactgtg tgttttcgag
     4021 attacccacg ttctttagat ttttgattgt ttaattatat tcttttacta aatgactatg
     4081 caaagatata ttgattaaac agtaccatgc aacacatagt actaaaatat tttatatttt
     4141 attgattttc aggttattta ttttttatta gggtaatgtt agggctaaag cccttgggct
     4201 tggcagaggc ttcctatcgc ctcactctaa agagtgaggt gagttgggat gataggagtg
     4261 ttcgctagcc tcaccatgaa gggtgaggtg aaaaagaaaa gccggaacag gatactcctg
     4321 ttccggctta tataagttgt aatcaaacac taatcaggtc tgtcgtgcaa aaacttattg
     4381 cctttgctaa ggcccggagt tttatcctgc tcattattga gtgagtagcg cgaatagttt
     4441 gtatcccctg acgacagtac gttctgcagt tttacattct tacgcatata tgcaggctct
     4501 ttttccattt cgtttaacgc ctgcggattc aatgttctgc tgttgagtga gcttttcata
     4561 gtctcgattc tttcgcgtgt gcgtctataa atatcgctat taggctctct gctttcctgc
     4621 gatggcactt cgggcaacga ctcctgcgac ttcggcaatt cgctaaccgt tggctcctct
     4681 gttaatgtag cttcgggtgc tgttatttcc tcatcctgag aaatcacatt cggaaatccg
     4741 gtgaactcaa attccatagt acttttatca gcaacaacct cctccggtgt attgtctttt
     4801 tcaaaaaatg ctgttgatgt aacaggtata tcctgctcta atgtaataag aacaggttca
     4861 ttcattacag gctcttcctc tacagttgtt tcactcactt cgttaaccac agaagttggt
     4921 gtttcatcat agatgtttaa aacaactttc tccggttgtt tcactttttc ttccttagtt
     4981 ttttccttat cggcattaaa gcctgtggca atgatggtaa ctgaaatttt atcgcctaaa
     5041 gaatcatcgg taccaacgcc cataatgatc tctgctgtct gcccggcttt ttgctgaata
     5101 taatctgtta tatcgcccat ttcatccata gtaatttctt ctttaccgct ggtgatgttc
     5161 aggagaatat gacgtgcccc ctcaatgttg ttatcgttga gtaatggtga gtcgagagca
     5221 agttctgctg cacgaatagc tctgccttcg ccagaagact gtgcagcacc cataatggca
     5281 acaccactgt cgcgcataac agtctgcaca tctgcaaagt caacattaat gtgcaaggta
     5341 acactgataa cttctgcaat actcttagca gcagtagcta acacattatc tgcataacca
     5401 aatgctgtat tcaactttaa gttgccatga atttcgcgca gcttatcatt acagataatc
     5461 agcaatgtat caacactgtt tttcagctct tcaataccgg catcggcctg ttgttttctc
     5521 ttgcgacctt caaatgcaaa aggcacagtt acaattccta cagtgaggat acccatttcg
     5581 cgggctacac ctgcaatgat aggtgcagca ccggttccgg ttccgccacc catgccagca
     5641 gtgataaaaa ccatcttggt attatcacta agaattgctt tcagctcttc aatattttcg
     5701 atggccgatt gtttgccgac ttcgggaata gatccggcac cacgtccttc tgtaagactt
     5761 ggcccgagtt gtattttgcg tggcacaggg ctcatctcca aagcttgtgc atcggtgttg
     5821 caaactacaa aatcaactcc tttgattccg agtttgtaca tgtggtttac agcattgctt
     5881 cccccaccgc ctacaccaat aacctttatg attgatggca gctcctttgg cataaattca
     5941 tagttcatca tcttaattta ttttttgttg gttatgaaaa tttttaactt aaattatttt
     6001 atcgtcagag tcgtcagtaa atattttttt ggtagaatca aaaattccgc tgaaccaaga
     6061 tcctttatgt tgtacacgtg gttcagaagt gtttttttgt cctgattttc tgtgtttatc
     6121 caaatcctga aaacctttaa tcactaatcc tacaccggtt gcataaatcg ggcttttggt
     6181 ttcttcggca ctttttgcca agtgctcgtt tggatagccc aatcgtgtat ccatgccggt
     6241 aatatattcc atcaactgtg tgatgtgtcg tagttgtgca ccaccaccgg tgacaacgat
     6301 acctgcaatt agttttttct cgaaaccgga agttttgatt tcataataaa cctgctcaat
     6361 aatttcttca acacgtgcct gaataatgct tgcaagattt tttacagaaa tttcttttgg
     6421 ttcacgaccg cgtaatccgg gtattgaaac aatctcattg tctttcatct gacttgataa
     6481 tgcagagcca aattttattt ttaataattc tgcctgattg cggataatgt ggcaaccttc
     6541 ttttatatct tctgtaatta catttccacc aaatggaatt acagcagtgt gccgaataat
     6601 gttgtcctga aaaatggcaa catctgtagt tccaccacca atatcaacta acacaatacc
     6661 tgcttctttt tcttcttcgg tgagaacagc ctctgcactt gccaaaggtt caagaattaa
     6721 atctgcaggt gtgagtcctg ctttagtcac acacttgtgg atattgtttg ctgctgtaac
     6781 aagtcctgta atgatatgac agttggcttc aagtctgatg cctgacatac ctatcggatc
     6841 ttttattccc tgctcattgt caacaataaa ttcctgtgga ataacatgaa taatttcttc
     6901 gcccggaggc attgccaatt tatacatgtc gtcaattaat gcatcaatat cacgctggct
     6961 gatttcatca tctgtgctgc tgcgtgttat catacctcta tgctgaaggc ttttgatatg
     7021 ctgacctgca ataccaacat gcacagattt tatattgcct ccacacttat gttctgcttc
     7081 ggcaacggct tgcttaatac tgcctacagt cttttcgata ttagcaacaa cacctcttgt
     7141 aacaccaaca gattctgctc ttccagtgcc tacaatttca attttcccaa actcgtttct
     7201 gcgacctaca atggctgcaa tttttgtagt gcctatatca agacctacta ttacttcatt
     7261 acctgttacc tgctctgcca cttattgtta gttttatgat agtttaacat ttttcttttt
     7321 gcatactacc tgacctttaa atttcaggtt gatggactca tactgatccc agcctgttgc
     7381 cggaaatccc tgacggtata aattgagaag aagattcagt ttctcatcaa gatttgatat
     7441 gttgccaagc aaaatataat tatttcccag acgaggtatc aattcaattt ccccgttctc
     7501 atctacataa atctgctcaa acattgcatc ccacgatgaa tcgcgcttta gttgttctgc
     7561 caacaaaaaa acctggctca gcataggtag ttttcctaca gaatcatcag gcaaaggcaa
     7621 ggaatttata tccaaaccaa gcttctgttg cactaagcct tcagcaatct gaccgcttgc
     7681 aaccatcaca ggctcggaat atttttcaga taatggcata aattttccct gattgtctat
     7741 ataaaaattt tctccttttg aattgattat ccgtactatc ggtgttcgtt gctgcacgtc
     7801 aatatgtacc catccatcaa tggttgaata tacctcagct tttaaaacgt gagggtttgc
     7861 atcaatgatt ttttccaaca taaaagtgtt aatatcagtc accttattac caatgatttt
     7921 tttgcctttg gaacgaacaa tttccatgat gtcgctgcgg tcaataaacc cctttacatt
     7981 ttcatcttcg atgttcacaa caacaccttt acaaacaaga tcgtggtttc gttttgttgc
     8041 aaaactcatc agagttagaa cagtggcggc aaccaaaaca tagccggcaa ttaataaaac
     8101 tgaacgcagt ttgtcgctaa tcttaaaatt ggttttcatt gtttatggtt tttaaattcg
     8161 tgaactgaat attttttttc aagcaattgc tgaatgggcg ttaccaacgt atcaatatca
     8221 ccggcaccta aggtaatgag cacttcaggt tcacgcttat tgatgtaatc caaaacatct
     8281 gtcttgttta agaatatttt attagcattt ttaattttat tcatcagcca tgacgaagtt
     8341 attccttcca acggtaattc gcgggcagga taaatatcaa gaagtattat ttcatcaagt
     8401 aaggccagac ttgcggcaaa ctcatcagca aaatcacgtg tacgtgaaaa taaatgcggc
     8461 tgaaagatgc ctgtaatttt ttttccttca aacaattcgt gtgctgacat gatagcagcg
     8521 cgcagttctt caggatgatg tgcatagtca tcaatgaaag tacaattagc atcagagaac
     8581 cggatgtcga atctgcgttt tacaccttta taactttgca gcgccattcg taccgattct
     8641 tctttaacac caactttcat tgcaagagca atagctgcaa cagcattttc aacattatga
     8701 cggccgggaa gtcctaaaga aagatcttca ataataccat caggcgaatg aaaattaaaa
     8761 caataacgat gattacggat tgaaatctta tctgcaaaat aatcggcctg tgaatctgca
     8821 gcatagaact tcgttaatgc cttaaccggt tgtagttgca atccgtttcg taaaacaagc
     8881 aatccttttt cgtcaacctg agaggcaaac tgctgatagg ttttctttaa ctcttcgcca
     8941 ctgccgtaaa tatcaagatg gtcggcatcc atacttgtaa tggcagcagc aaaaggaaat
     9001 aaggtaagaa acgaacggtc aaattcatct gcttcaacca cattaataac ttcatgttca
     9061 ctgacaggaa gaataatgtt gctgttataa tttcttgcta ttcccccaag aaaagcagcc
     9121 acattaatat ttgctgtttt aagaatatgg gccaaaatac ttgaagtagt ggtcttgcca
     9181 tgtgtgcctg ctacggcaag tgttttatgc attgaagtga tgacacctaa aacctgtgag
     9241 cgttttacaa cagtaaactc ctgctgactg aaaaaattaa gttctgaatg atctttaggt
     9301 attgcaggtg tgtaaacaac cagaatatta ctctttctgt ttgcctccat aaatgctaaa
     9361 ggtatagctt gtacatcatc attaaaatgc acgtctatat tttcgccttt taactcctca
     9421 gttaatgctg tttgagtttt gtcataaccg gcaacttgtt tgccgatagc tttaaaataa
     9481 cgtgcaagcg cactcattcc aatgccgcct atgccgataa aataaacatg tgttatattc
     9541 tcaaatttca tgagcctgca gttacatgtt taaggttttg tgctgcatgt tgcgtagcca
     9601 ttgcaataac ttcgtcagca attttttgtg ctgcatgagg cagtgccatt gctgcagcgt
     9661 tggccgaaag ttgtttcaaa cgatcggcat cattaataat gttcaatgct gtatcagcta
     9721 agtgttgcaa tgcatcggta tcttttacta aaaccgctgc gtctcttttc accatggctt
     9781 caatattttt tgtctgatga tcttctgctg cagtaggcaa tggaaccata acagaaggtt
     9841 tacctgtcaa agtaagttca gaaactgtgc ttgcccctgc acgcgacacc actaagtttg
     9901 cagcagcata ggcaaaatcc attcttgcaa taaaatcaaa aacacgtgct tgcccatagc
     9961 ctacttccgc aacagcacgc tgtgcctctt cataaaacag tttgcccgtt tgccatatca
    10021 gttgcactcc agcagctttc agcttttcta atccggcaag aatactttta ttgatagagc
    10081 gtgcgccctg actgccacct acaatgagca aagttttttg tgaaggatta aaactaaaaa
    10141 aagacattgc ctcatcaaac tttccatcaa gcatcagcac attttctcta accggatttc
    10201 cggtgagaat tatttttgat gcagggaaat atttatccat gccttcatag gcaacacata
    10261 ttttctgaac acgtttggca agaattttat tggtgatacc tggataggaa ttctgctcct
    10321 gaatgagaca aggaattttt tttaatgatg cagcgtaaag taaaggtccg ctggcataac
    10381 cacctacacc tacagctgca tttggtttaa actcctttaa aatatgcata gccttactga
    10441 tgcttgacaa caccttgaac ggaaatgaca ggttgtctat tgtcagctct cgttttattc
    10501 cgcttatcca aagtccttca attttataac cggcagcagg aactttttcc atttccattc
    10561 ggcctttagc accaacaaaa agtatttcag cctgaggtct tgcactttta attgcattgg
    10621 caatggcaag tgcaggaaaa atatgtcctc ctgtacctcc tccgctgatg ataactttta
    10681 tgtcaggctg ctgcatgtac tactcctgtt tttaactctt cctgctcaat attccggctg
    10741 acactaagaa taataccaac ggcaatgctg gtaaaccaaa tagatgttcc gcccatactc
    10801 aacatcggca atggctgccc cgttaccgga aaaagattta ctgcaactgc catatttatc
    10861 atagcctgga atactaggct gaatgcacag cctattgcaa gcaatgttgc aaaagcaaat
    10921 ggactctttc gaacaatgac gacagcccga taaaacagca acagataaag aacaactatt
    10981 accaaaccac caacaaaacc atactcctct attattattg caaaaataaa atcggaataa
    11041 ggatgtggaa gaaaatttct ttgcgtactc tgtccgggtc ctttaccaaa aacaccacct
    11101 gaggcaattg caatttttgc ctgttgtgtc tgatagtttc cctggtcatt tccattgctg
    11161 aaattttcaa tccgtttttt ccatgtacca attctccctt cgtatcctga aaatgaggca
    11221 accaataaaa acaaacccag ccctgcaata ccgataccta caagagaaag tatatacttg
    11281 aaatttatac gaccgaaaaa catcagcaca agacaggtgg taaacaatac tgctgcggta
    11341 gaaaagttag ccggaagaat taatccacag atgatacaaa caggaagaat tacaggaaga
    11401 aaagcagact taaagtcttt gatgttatcc tgttttttag aaagcgatcg tgctacatac
    11461 aacaacaatg caatcttagc taagtcagat gtctgaaacg aaagattgat tacgggcagt
    11521 gtcagccaac gtgatgcttc gttaaggtta gtacctgtaa gaagtgtaag aaacaaaagt
    11581 ggaatagcaa gaaaaattgc tatgcgtgaa acttttgcat agaaagtata ttttacaaga
    11641 tgtgtaaaat acattatacc aaaaccaaga aaaacaataa taccatgctt aaataaataa
    11701 tactctgtat taccttcctg atacttatat gccaaagtgc ctgtggaact ataaacggcc
    11761 ataagtgaca ttaacgaaag cagtattacc acaatccaga tgatgcggtc gcctttaaaa
    11821 tatgtttcta tccaattcat tttttttgaa ttaaatctta aagtgctttt acagcagctt
    11881 taaactgacg accacgatcc tcgtaatttt taaacaaatc gaaacttgcg cagcatggtg
    11941 acaacaatac tacatcacct tttttgcctt tctgataggc aacgcttact gcttcctgcg
    12001 cagacaaagt atcagtaata tcgcttacaa tacttccgaa tgcttcatgt atttttttat
    12061 tgtctttacc aagacaaaca atagctttca ctttctcttt tacaagatcg ttcagcattt
    12121 cgtactcatt acctttatca acaccgccca gaatccaaac aacaggtttc tgcatgcttt
    12181 cgagagcaaa ccatgcactg ttaacgtttg ttgcctttga atcgttgata aagtcaatac
    12241 cgtgtacggt atttacgaac tccatccgat gttctgcatt gcgaaaatct gcaaagcttt
    12301 cgcggataga ctccttgcga atgtcgagta tgcgtgctgc aactccggca gccatactgt
    12361 tgtagaggtt gtgtttgccc tgtaaggcta gttcgaaaat agacatagta aataagttgt
    12421 tgtgtatgtt cagatataag ttattgtcat ttacaaaagc actcatgcct tcatcctgcc
    12481 tgattgaaaa aggatagcac gttgccaaag gatttctttt cagaacttct gtttttacaa
    12541 ctgcatcatc gaagcaatag ataaatgcat cttcggcagt ttgattctgt atgattctga
    12601 atttagcgtc aatataattt tgtattgaat actcgtaacg gtcaagatga tcgggcgtga
    12661 tgttgagtaa cacagcaata tcagctttga aggtgtacat atcatctaac tgaaagctgc
    12721 ttaactcaag cacataatgg tcaaaatttt cttctgcaac ctgccaggca aaactcttac
    12781 ctatatttcc ggcaagacct acattaacac ctgccttttt aagaatatga taggtcagta
    12841 aagttgttgt tgacttgcca ttggtgcctg tgatggcaat cattttagca ttggtaaacc
    12901 ttgaagcaaa ttcaatctct gaaactatat tgatgtttgc actcttaatt tttttaatca
    12961 ctgctgcctt atcaggaata ccggggcttt taactacctc atctgcatta agaatcagac
    13021 tgtcggtatg gcaaccttct tcaaactgaa tattccgctc tgtcagatgt gttttgtaat
    13081 tgtcggcaat acgcgatagg tctgatacaa aaacatcata gccttgcttc tgcgcaagta
    13141 tggctgcgcc tactccgctt tcggcagcac ccaaaataac tatgcgtttg ttttttgaca
    13201 tttacctatc gcagttttaa tgttaccaga ctgattacag ccagcatgat tccaacaatc
    13261 cagaaacggg ttacaatttt agattcatga tatccaagtt tctgatagtg gtgatgcagt
    13321 ggcgacattt taaaaatgcg tctgccttca ccaaattttt tctttgtgta tttaaaataa
    13381 cttacctgca tcactacaga aagattctca atgagaaaaa caccgcagat aacaggaatc
    13441 atcaattctt ttctgatagc aatagcaaac actgcaataa tgccacctaa ggcaaggctt
    13501 cctgtgtcgc ccataaacac ctgtgcagga taggcgttga accataaaaa tcctatacat
    13561 gccccaacaa atgccgacat aaaaacaagc aactcacctg catttggaat gtacataatg
    13621 ttaaggtagt tggcaaaaac cgtgttaccg gatacgtaag caaaaactgc aagtgccaaa
    13681 ccaataatgc ctgagctgcc ggcagcaagt ccgtcaatac catcagtgat gtttgcacca
    13741 tttgaaactg ctgtaacaat aataattaca acaggaataa aaataagcca tgcaagattt
    13801 ttatatccat cacccaacca tccaagtaaa gaggcataat caaactcatt attcttgaaa
    13861 aacggaattg ttgtttttgt tgatttaata tttgtggttg aaaacaaaac tccttcctga
    13921 ctttgaagat catcaactaa agtattgtca tgatgccagg taactgcaga acgaactgct
    13981 ttttcgcgaa caactacatg atcattaaaa tacaaaacac tgccaacgat gagaccaaga
    14041 cctatttgtc cgagaacttt aaactttcct gcaagaccct ttttatcttt cttaaatact
    14101 ttaatatagt cgtcaagaaa accaattgca cccagccata ctgttgaaat caacatcaga
    14161 atgatgtaaa cattaagcag tttagcaaac aaaattgttg gaataagaat agccgaaaga
    14221 ataatgagac cacccattgt aggtgtgcct tgcttttgca cttgtccgtc aagtccaaga
    14281 tcacgaatgg tttcgccaac ttgtttttta tgcagataag caattattgt tcgtccgaga
    14341 ataaaagaaa acaacaatga aaacataatt gccaatgctg ctcggaagga gatgtattta
    14401 aacactccca ttccgggcaa atttgtatgt tgatctaaat attcaaagag atagtatagc
    14461 atcaggcgtg taggttatta aagttttcct gtaatatttt tttatcgtca aaagggtgtt
    14521 tcactccttt aatttcctga taggtttcat ggccttttcc tgcaatgagg ataatatctc
    14581 ctgcagcagc aaggttgcat gccacacgaa tggcttcatt gcgatcggtt attgccatca
    14641 ctttagatga taaatgtgcg ggcacaccgg cacgcatctg ttcaatgatt gcttgggggt
    14701 cttcgtttcg cggattgtca gaagtaagaa taaccttatc actcatctcg caggcaatgt
    14761 gagccataac agggcgtttt gctgtatcac gatttccacc gcaaccaact actgtaatta
    14821 ctttctcatt tcttgttctt atttcgttga tggtagaaag tacattattc aatgcatcgg
    14881 gtgtatgtgc ataatcaaca atgccagtaa taccggaacc ggaaacaaca taatcgaatc
    14941 tgccattgac agattcgagc atactcaatg cagtaagcac tcgctgctta tccaatccta
    15001 atgaaacagc agttgcataa attgctaaaa gattataggc attgaatgca ccaataagtt
    15061 tacacaacac ttctgtatta tcaatctgca gcaacagacc actaaaatta ttttcaatga
    15121 ttctgcatct gaaatcggca ataccatgca gaccataagt ttttttggca gcatgtgtat
    15181 tctgaaccat caccattccg tttttgtcat cggcattagt caatgcaaat gcactttcag
    15241 gaagaccgtc aaaaaatttc ttttttgctt tcagatattc ttcaaaagtt ttgtgatagt
    15301 caagatgatc gtgtgtgata ttggtaaaaa caccaccggc aaattctaat gctcctattc
    15361 tgttttgtac cacagcatgt gagctgactt ccataaagca gtactcacat tcttccaaca
    15421 ccatcatgct taatagctga ttgagtttta ttggatcggg tgttgtatgt gttgcaggaa
    15481 taacagtatc gttaactctg ttttctacag tagaaattaa tccgcaacga atattcagtt
    15541 ggcggaataa tttatagagc aatgtggctg tagttgtttt accattagtt ccggtaattc
    15601 caactaattt tagagaagaa gaaggatggt gataaaagtt tgcacacact tcagaaagtg
    15661 ccagataggt attttttact tttatataat tgatacctgc ctgaatgaga tctggcataa
    15721 cttcgcataa aactgaagta gcgccttttt ctatggcttc taaaataaaa gcatgcccat
    15781 caacattggt accgcgaagt gcaacataca tttctccggg tttaattacg cgtgagttaa
    15841 tgcatattcc atatacagct acattggtat ttccaatgac ctcctctgtt ccagctttgt
    15901 ataaaacttc tttcagtaac tgcatcaact taactctatt gttattacct gacctctata
    15961 aattcttgtt cccgcctgta atgattgctt caccacagca ccacgtccaa ctactttaac
    16021 ctgcaatcca tttgtttcga gcatataaat tgcatcacgc aaacccattc cttcaacatt
    16081 tggaacagta ttatctttaa tgctaacagg cttcaatgct gcaacagctt ctctcttatc
    16141 aactgatgac cattttgatg cagtgccaac agtttttaca tcaacaccaa cattgttaca
    16201 tgcagcgatt gttgctttag tcagacctgc ttttaccttt ggtaattctg ctatttgcac
    16261 atcaagtcct ttgtgcatat cgagattggt tgcataaact ttatctgcaa tatctttgaa
    16321 tacgggtaat gctacctcgc caccatagta acctgccgtt gaagggttat acactactac
    16381 aatacatgaa tatgcaggct tgtcagcagg aaaataacca cagaacgatg cctgatatct
    16441 tactcttcca ctcttataac cacctttatt atttgccatt tgtgcagtac ctgttttacc
    16501 ggcaacggca tatacgcagt ctttcaactt gcgtccggta ccattctgaa ctacaccttc
    16561 gagaagttct cttgcttttg ctattgttga ttttgagcaa atagaatctg caataacctg
    16621 tgtcttatat tctttaacag catgtccacg gtagcgaatt tcttttacga aaaggggctt
    16681 taccattctg ccattattgg caacagcatt atagaaagta agtgtgtgca atggtgttac
    16741 catggtttca taaccaatag acatataagg caatgaaatt ttgctccact ccttacttgt
    16801 agttgactta ataagcggag tagcctctcc tgcaatctga atattgagtt tatctccaaa
    16861 atgcatttta tgtacacggt caataaatgc ctgttgattt ttccggtaat acttctgaat
    16921 gagtttagaa acgcccacat ttgatgattc ttcaaatgca tgttttgcag ttatcttatt
    16981 caatccctca tgcgagtcgc gcataatcag tccgggagca aaacttgttc ttccacctgt
    17041 ggcatctatg gtatcttcaa ggtcaatata accatcttct attccggcca tcatactcac
    17101 taatttaaaa gtggagccgg gttctgtggc atagcctata gcataattaa aatcttctcg
    17161 ataataaccg gagtcagctt ttttcagatt tacaattgct tttatctctc cggtagcaac
    17221 ttccatcagt atggcacatc ctgcatcagc atcgttcttc accaactgat tgtagagtga
    17281 ttgttgtgca acatcctgta tgttcaaatc tattgatgta taaatatcac agccatcttt
    17341 tgcttcaact tcatcttctg cattgagcgg tttccaaatg ttacccgata ttttttgcat
    17401 caggcgcttg ccactttctc ctctcaaact ttggtcgaag gcgccttcaa ggccaacagg
    17461 ttgaacattt ccaattttaa aaccaattgt tctggaagca agttccttga aaggtttctg
    17521 acgcatattt ttttgttcaa caatgagacc acctttataa cggcctttgt taaaaatcgg
    17581 aaacgtcttt acttttttca gttcagcata gcttacatta cgtttaacaa gcaaatagcg
    17641 ttcgccatcg cgtctgcctt cgcgaagcat tctcttgtac tgatcttttg ttttatcctg
    17701 aaacaatgca gcaaggttta aagccagtga gtcaatacct gcattaaata ttttatctgt
    17761 aatactttct gccttcatat
//
LOCUS       DAOOOL010000042        17203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_266_length_17203_cov_94.428388, whole genome shotgun sequence.
ACCESSION   DAOOOL010000042 DAOOOL010000000
VERSION     DAOOOL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_17203_cov_94.428388"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKU82_05175"
     CDS             <1..661
                     /locus_tag="PKU82_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ82301.1"
                     /translation="AAKKLKKFKEKFFVNKYAKLKVELILQQGLASARAMETASEKKV
                     DMIVMGTTGTGAGERFLIGSNAGRVVKNAPCPVLLIPPRVKFNGLKKLVYATDLSDDN
                     LSKASELIPFAKAFNSELLFLYIDTTTLSKDEIDVKKITDLVRKKVKYPKQSGYVCTD
                     VSIQNGVSHFLKKFKADALVMYTRHRNLFKGLYKPSIAKSVSYNTKVPLLVVHENDTL
                     F"
     gene            812..4072
                     /locus_tag="PKU82_05180"
     CDS             812..4072
                     /locus_tag="PKU82_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HOZ82302.1"
                     /translation="MQNLIKSKLAMLIIAVFLYGQSIAGNNCWKPVEEVSLSGMGKMN
                     RNTIPSTARYYELNMQQLQSLLQQAPNRGQQSNLIIDFPDAYGNMNAFRVYASPIMPE
                     MLSSKYPMIKTYCAQGINDPTATMRFSVTQFGLHCMTLSGTNPTNYIDTYTKDLNYYI
                     VYDKASLANNVRNFECLTDEDAELPSMRNGVAGINNNTDDQLLRTYRLALTCTAEYGN
                     IFATTPGSEKADIMAQMTITMNRVNGVYERDLAITMEFVPNNDTLIFYGNTSQDPWSN
                     EWNTKTAQVCDQYIGVANYDIGHNFNTTGGGNAGCIACVCLSSSQSGTHKGRGMTGSA
                     NPVGDPFDIDYVAHEMGHQYGGYHIMNTCSRSGSGNTEVEPCSGSSIMGYAGICSVNI
                     QAHSDDDFNYVNVRDISANIQNGSSTCAVTTPLTDNPPTADAGADYTIPKGTAFVLEG
                     SATDADGMASLTYNWCQNDAAQAPSNSSPQPTWAVGPLSRAIKPGTSPNRYMPNLRSV
                     VLKNLYPTWEKTSDVARTLNYSFIVRDNDVNGGQTASDLMQVTVASAGPFVVTSQNTP
                     TNWNAGASETVTWNVAGTDIAPVNTPFVNIFLSLDSGFTYPYTLATNVPNNGSASIVV
                     PAGAASQWGRVMVRGAGNIFYALNGSYINIQASEFVMNMSSTAQSVCPPNDITYNFMY
                     NTFLAFNETTVFSITGLPAGATGTFTPATAVNDSTPVQLLISGLTPSMTGTYNLTITG
                     TSPSVTKTTSVTLDILDVNLPAATLTLPADGSSGIASIATLTWQTAASPGTLYDIEVA
                     TDSLFTNVIASATGISTNTFTTTPLLSSTTYYWHVKSYNSCFANSYSAAWSFTTNNCS
                     NLASANVPVVIPSSGAPTVTSTLNIASAGTITDVNVVGLNGTHTWISDLTISIKSPQN
                     TTVTLFDQICTNENDFDCNFDDAAAPGALPCPPIGGGTYNAVTPLSVFNGQQAAGNWT
                     LTVTDHFNSDGGSLNGWGLEICTTTPTGIETHIVSTSMILYPNPVKELLNVNVAGKKG
                     EQVLVEIKNITGQVIKSISVNANTKISIDVKDLSAGMYFVTATGSNIETQRFIKAK"
     gene            4189..6606
                     /locus_tag="PKU82_05185"
     CDS             4189..6606
                     /locus_tag="PKU82_05185"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOZ82303.1"
                     /translation="MQINFKKLIPHGIAISIFLLLTAVYFLPLLEGKGLSQHDIAQWT
                     GMSKELTDFRAKYHVEPLWTNAMFSGMPAYQISTLYPANLIQYVNDLLFFFLPSPASY
                     VFLALLSFYILMLVLKFDYRMGIAGAIAFAFSSYNFVIIAAGHNSKAHAIALMPLVFA
                     GIILTMRGRFWFGGAMTAMALALQLYANHLQITYYLMIAILVMVIAYFIDAIMKKEIG
                     GFIKSASVLAIAAVIGILPNYTSLAATYEYGKYSTRGPSELTSKKESSGLDKDYAFSY
                     SYGISETFTLIIPRFHGGASVSPLDENSATYKALTSNGVPVQQAKSFIQAVPLYFGNM
                     LSTSGPPYAGAIICFLFVAGLMLIKGKEKWWLLVATILSFMLSWGENFLSFNELFFYH
                     FPGYNKFRAVSMILTIAQFTMPLLALLALKEIFDGKTNKNDILKSLKMALYITGGFCL
                     LFTLLPGMFNDFSSQADEQLKQYDWLITAIKQDRESAVRMDALRSLFFIAATFGVLWF
                     MLKDKIKATVAIITIAVLFTVDLWPVGKRYLNDNNFVAKSKTQKPFEPSPADTQILQD
                     TTPGFRVLNTTLNAFSDASTSYFHRSIGGYHGAKLKRYQELIENCISKNNMSVLDMLN
                     TKYLIVSDKEQQPVAQLNPGAIGAAWFTDTVKMVANADEEINALNSFNPRTIAFVDKR
                     FENTLKSFSPQHDSTATVKLNSIVCNHLVYETQTALPQFCVFSEIYYDKGWNAYIDGQ
                     KADYVRANYVLRAMVVPAGKHKIEFKFEPEVYFTGEKISLAGSIVLLLLFAAGAWIEL
                     KSGTEKK"
     gene            6619..7923
                     /locus_tag="PKU82_05190"
     CDS             6619..7923
                     /locus_tag="PKU82_05190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82304.1"
                     /translation="MKKVLIITYYWPPSGGAGVQRWLKFSKYLRDFGWEPIIFTVANG
                     EFPEQDNSLLKDIPSNIKVIKQPIWEPYLLYKFLTGKKKHDKIHAGFLTEKKNPGMLQ
                     NLSVWIRGNLFIPDARSFWIKPSVRTLTKWLNENKVDAIVSTGPPHSCHLIALKLKRK
                     LNLPWLADFRDPWTNIDFYKDLKLSSWADAKHHRLEKEVVQKADAVVTIGKTMQEEFS
                     ASFKRNIHCITNGFDHDDILNTSATVDLKFSIAHIGTMVKTRNPIVLWKALSELLTEI
                     PKLKADLEIKIVGKADLEVSQSLENFALTTFINRIDYMPHEEVVKLQQQSQVLLLVVN
                     NTPNAKGVLTGKIFEYLLAKRPILCIGPEDGDAAAIINQTQAGFVAGFNDVNRTKQIV
                     KDLYNKFKENNLNIQSQNIDQFSRKNLTKQLALVLNDIAHLS"
     gene            8068..9213
                     /gene="wecB"
                     /locus_tag="PKU82_05195"
     CDS             8068..9213
                     /gene="wecB"
                     /locus_tag="PKU82_05195"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOZ82305.1"
                     /translation="MFKLLTIIGARPQIIKAAAISRAIKKSFSDKIKEVIVHTGQHYD
                     EKMSAIFFDELNIPREDYNLKTGSGSHAVQTAEIMRLLESVLEKEKPNAVLVYGDTNS
                     TLAGALTAAKMHIPVVHVEAGMRSFNKSMPEEINRICCDHVSTLLFSPTKTGVNNLCR
                     EGFSEHTLPPFNINNPKVYFCGDVMYDNSLYFAAQAKSQSSILSKSGIVKNDFMLCTI
                     HRDSNTDNPKNLSEIFRALIKISETQQQNIVIPLHPRTAKVLEQNLSPELFQQVNNST
                     LLKIIEPVSFIDMISLESECTMIITDSGGVQKESYFFQKPCIILRNETEWIELVEAGT
                     AIIAGPDYAKIVDAYNQLASSKLSFAPLFGDGKASEFICSEMLSVLN"
     gene            9240..9944
                     /locus_tag="PKU82_05200"
     CDS             9240..9944
                     /locus_tag="PKU82_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82306.1"
                     /translation="MKYILSKSSYIKSLQCLKSLYFYKFNYKDRDPISAEQQAKFDRG
                     HQYGKIAHQLFPGGVDVSPPSPFEYATSAKQTHSLLLKQQPVIYEAAFIANEVIVALD
                     ILVRKGGLLHAFEVKSSTAISQTILNDAALQYYVITQCGFELEDFSIIHLKPDYKELP
                     VDDVKALFVSESVKEWCVSQQQFVEDNILAAKEIIRNKKMPNKKQGEHCNSPYPCDFK
                     NICNKSNSFDSGSLFG"
     gene            complement(9946..12543)
                     /locus_tag="PKU82_05205"
     CDS             complement(9946..12543)
                     /locus_tag="PKU82_05205"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOZ82307.1"
                     /translation="MKKSLLFFTWVSLCLTVSNQLNAQQWIAQSSNINPNNYVQFIDA
                     VDTNVVWGLSSDRNSQQNPVQEFTRTTNGGLTWTAGTITNAANHGPSCIMALNADTAW
                     VAMFNVSGGGSILRTNDGGVSWTKQTTATFTAPNGFPNVVHFFNANEGFCMGDPNGGY
                     FEIYTTTDGGNNWIRVPQANIAANLTGEFGITDVYTNYGDSTVYFGTNKGRIYNSTDR
                     GMNWTVSSTPYTGFIGAITFRDANFGIACEADNATTSTDLIVTNDGGATWSLLPTSSA
                     GFTMKQSIRYVPGTDSTFVITSPYTIYGSAFSIDNGVNWRLMDNLIHSDCDFVNATTG
                     WSGGGELNSPIYKWTGPMTIAANDAMVKSIDVRSAVGTGTQTPMATVLNNGLTSQTFN
                     VTMDISGGYTSTKTVSNLAFNQTQQVSFDPWTPGVTGSYNITVYSQLTTDSDLSNDTL
                     NKTTTAYNELPNYGWTSKTPATIATFGGASAFIPSVNAPLDSGYLFSDGGSDLSIIQP
                     KNEKFDEITNSWTSATDMPLGVYQFSMHGVQNMMYAIGGYSTGFVPSATNYIYNSQTN
                     SWTSGAVMPQPVGDYVSGVYNDSLIYFIGGYDGTSDLNAVQIYNPATNSWSMGTPKPG
                     TAVSGFRGGIYNNYIVVTCGYSQTLQGAIDETWIGQIDPANPTNITWQAAAPYPGGTM
                     TRFAGGGTFKNSLPYVVFAAGDPTGVGSEVKDDVWAYDLNNGEWLIGPPKPTACSNVS
                     NFTGVVSNDSLYMVVVGGFDGTTTTGANEWLNLGASPFVGVKELNNQLEVSVWPNPVS
                     DILTVLMPSPLEMTKLTVFDLKGRMVKEIQANGSKIQIVVRDLDNGVYILKLSDKKNS
                     NTARFTVMH"
     gene            complement(12681..14879)
                     /gene="pnp"
                     /locus_tag="PKU82_05210"
     CDS             complement(12681..14879)
                     /gene="pnp"
                     /locus_tag="PKU82_05210"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOZ82308.1"
                     /translation="MNLGVKHTFTLPDGREISIETGKLAKQADGSVVVRMGDCMLLAT
                     ACAKQDAMPGVDFMPLTVDYQEKYASVGRFPGGFFKREARPSEYEILISRLIDRALRP
                     MFPENFHADTQVLVTLHSGDSTVYPDALAAFAASAALTISDIPFNGPISEVRVAQIDG
                     AMVINPYIADIARATLDLIVAGSMENIVMVEGEMSEVSEATMLEAIKVAHEAIKIQCK
                     AQLDLLEKIGGKTKREYNHEEHDEDLRKVVRDETYQKVYQVALQGNPNKNERKKSFEA
                     IVEEFKTRFTEEELEEKSAFINHYYHEVEYDAVREALLAEKKRLDGRGLTEVRPIWSE
                     TNYLPTAHGSAVFTRGETQSLTSVTLGGKMEEQIIDGAMIEGTKKFLLHYNFPPFSTG
                     EARPMRGTGRREVGHGNLALRALTKALPPESENPYTIRVVSDILESNGSSSMATVCAG
                     SMALMDAGIKMKSTVAGIAMGLISDGKGRYAILSDILGDEDHLGDMDFKVAGTRNGIT
                     ATQMDLKVTGLPYEVMAQALEQARQGRLHIISEMEKTISEARPDYKPNAPRIVEFTIE
                     TDQIGAVIGPGGKIIQEIQRESGATITIEEKNNKGYVQIAAVGKESMDKALERVKAIV
                     AVPEIGTVYKGKVKNITTFGAFVEIMPGKDGLLHISEITHERLATMDGVFKEGDLVEV
                     KLIGVEEKSGKLKLSRKVLLPKPERQPGDAGKPNDGKPRERRMPRENQNS"
     gene            complement(14958..15227)
                     /gene="rpsO"
                     /locus_tag="PKU82_05215"
     CDS             complement(14958..15227)
                     /gene="rpsO"
                     /locus_tag="PKU82_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOZ82309.1"
                     /translation="MIVTSERKKELFKQFGGNDKNTGNSEAQIAMFSERINHLTDHLK
                     IQPKDYATQQSLIKLVGKRRSLLNYLHKTDITRYRKIVADLGLRK"
     gene            complement(15374..16282)
                     /locus_tag="PKU82_05220"
     CDS             complement(15374..16282)
                     /locus_tag="PKU82_05220"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOZ82310.1"
                     /translation="MKKIITTVIAVAFAGSTMAQTSAPVDTSWKKGGMGSINFNQVAL
                     SNWAAGGENSMSGAAFLTLFANYAKDRLAWDNQLDLAYGLLKSGDAKVRKNEDKIELN
                     SKVGYKVSHESKFYYTFLFNFKSQFANGYDYKNDTLLKYPISKFLAPGYLLYSIGIDY
                     KPNDAFSLYLSPLTGRTIIVNDDMLAQAGAFGVDPGKKSLTQLGAYLRAMYKKDVFTN
                     VNFQTKLELFSNYLDNPQNIAVNHEILIAMKVNKFITANIGTQMIYDDIIPVTVIKEK
                     SGVKETKTGPRLQFKEVFGIGLSYKF"
     gene            complement(16370..16798)
                     /gene="mscL"
                     /locus_tag="PKU82_05225"
     CDS             complement(16370..16798)
                     /gene="mscL"
                     /locus_tag="PKU82_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOZ82311.1"
                     /translation="MGFLKEFKDFAMRGNLVDFAIGVVVGGAFGKVTTSFVDGIVMPI
                     VGKLVGGADFSELKYKIQDGSKEVLDAAGNITTKAVPEVFIKYGEFITVCLNFLIIAF
                     VMFLVIKAMNNLKKEEPAPAPAGPTKDQELLGEIRDLLKK"
     gene            complement(16919..>17203)
                     /locus_tag="PKU82_05230"
     CDS             complement(16919..>17203)
                     /locus_tag="PKU82_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOZ82312.1"
                     /translation="IAIDPANGQLITSSISAQTTRIMQNIEAILLAAGLNFSNIVKTT
                     IFLTSMSDFDEVNKVYGSYFKDNFPARETVAVAGLPKGVNVEISVTAEVS"
BASE COUNT         5384 a         3438 c         3049 g         5332 t
ORIGIN      
        1 tgcagcaaaa aaactaaaaa agttcaaaga gaaatttttt gtaaataagt acgctaaact
       61 caaagtagag ttgatactgc aacaaggatt agcatctgca cgcgcgatgg aaactgcctc
      121 tgaaaagaaa gttgacatga ttgttatggg gactacaggc accggtgcag gcgaacggtt
      181 tttaattgga agcaatgcag gtcgtgttgt aaaaaatgca ccatgtcctg tacttcttat
      241 tccaccgcgt gtaaaattca atggactaaa aaaacttgtt tatgcaactg acctttctga
      301 tgacaacctg agcaaagcat cagaactgat tccatttgct aaggcgttta acagtgaact
      361 tttatttctt tacattgata caacaacttt atctaaagat gaaatagatg ttaaaaaaat
      421 aacagatctg gttcgcaaaa aggtaaaata tccaaaacaa tcaggatatg tatgtactga
      481 tgtttctatt cagaatggtg tgagtcattt ccttaagaaa ttcaaagctg acgccttggt
      541 gatgtacacc cgtcatcgca acttatttaa agggttatat aagccaagta tagcaaaaag
      601 cgtgtcctac aacaccaagg ttcctttgct ggtagtgcat gaaaacgaca ccctttttta
      661 gccttataag gtactgagat tactcattta ttaactgatt gcaaactgta attgtacttg
      721 tttctgttac tgctatatta gcggaataat cggtgcatga ggtcatttca tacaccattt
      781 ccaaatcaaa caaaaacaaa tcaaattaca catgcaaaac ttaattaaaa gcaaattagc
      841 catgctaatc attgcagtat ttctctatgg gcaatccatt gcagggaata actgctggaa
      901 accggttgaa gaggtttctt tatcaggaat gggaaaaatg aatcgaaaca ccattccgtc
      961 aactgcccgt tattatgaat tgaacatgca gcagttgcaa agtttattac agcaggcacc
     1021 aaatcgtggt cagcagtcaa atctgattat agatttccct gatgcttacg gaaacatgaa
     1081 tgccttcaga gtttatgcat caccaataat gcctgaaatg ctttcatcca agtatccgat
     1141 gattaaaaca tattgcgcac aaggtataaa tgacccaaca gcaacaatgc gttttagtgt
     1201 tacacagttt ggtctgcatt gtatgacttt atcaggaaca aatccgacaa attatattga
     1261 cacttataca aaggatttga attattacat tgtttatgac aaagcttctt tagccaataa
     1321 cgtccgcaat tttgaatgtc tcactgatga agatgcagaa ctaccaagta tgcgaaatgg
     1381 tgttgcaggt ataaataaca acactgatga tcagttattg agaacctaca gacttgcact
     1441 tacctgcact gccgaatatg gaaatatttt tgccacaaca ccgggaagtg aaaaagccga
     1501 tatcatggcg caaatgacaa ttaccatgaa tcgtgtaaat ggtgtttacg agcgcgacct
     1561 tgcaattact atggaatttg tgcccaataa cgacacattg attttttatg gaaacacctc
     1621 tcaggatcca tggtcaaatg aatggaatac aaagacagca caggtttgcg atcagtacat
     1681 tggtgttgcc aactacgaca ttggacataa ctttaacacc acaggcggtg gtaatgcagg
     1741 ttgtattgcc tgtgtttgtt taagctcttc gcagagtgga acacataaag gtcgtggcat
     1801 gacaggaagt gcaaatcctg ttggcgatcc attcgatatt gactatgttg cacatgaaat
     1861 gggacatcag tatggtggct atcacatcat gaacacatgc agccgcagtg gcagtggaaa
     1921 cacagaagta gaaccttgca gtggtagctc aattatgggt tatgcaggta tttgttcagt
     1981 aaacatacaa gcacatagtg atgatgattt taattatgtt aacgtaagag atatctctgc
     2041 aaacattcag aacggtagca gcacttgcgc agttacaaca ccattaacag acaaccctcc
     2101 tactgctgat gcaggagctg attacaccat cccaaaagga actgcatttg tattggaggg
     2161 ctctgcgaca gatgctgatg gcatggcatc attaacctat aattggtgtc agaatgatgc
     2221 agcacaagct ccatctaaca gcagcccaca accaacatgg gcagtgggcc ctttgtcacg
     2281 agcaataaaa cccggaacat cacctaaccg ttatatgccc aacctgagaa gtgtagtgct
     2341 aaaaaaccta tatcctacct gggaaaaaac atccgatgta gccagaacat taaactattc
     2401 atttattgtt agagataacg atgtgaatgg tggtcaaacg gcatcagacc ttatgcaagt
     2461 aactgttgct tctgcaggtc catttgtagt tacttcacaa aacacaccta ctaactggaa
     2521 tgcaggtgct tcagaaactg taacatggaa tgtggcaggc acagatattg ctcctgtgaa
     2581 tacgccattc gtaaatattt ttctttcact cgacagtgga tttacttacc catatactct
     2641 tgcaaccaac gtaccaaata acggaagtgc tagcatagtt gttccggcag gtgcagcatc
     2701 acaatgggga cgtgtgatgg tacgtggtgc aggtaatatt ttctatgcat taaatggcag
     2761 ctatatcaac attcaggctt cagaatttgt aatgaacatg agcagtactg cacaaagtgt
     2821 ttgccctcca aacgacatta cttataattt tatgtacaac acatttctgg cattcaatga
     2881 aacaactgtg ttttctataa cagggcttcc tgccggtgca acaggaactt ttactcctgc
     2941 cacagcagta aatgacagta ctcccgtgca attgctgatt tcaggattaa caccttccat
     3001 gaccggaact tataatctta ctatcactgg tacatcacca tccgtaacca agactacttc
     3061 tgttacactt gatattttag atgtaaactt acctgcagca actttaacac tacctgctga
     3121 tggaagcagt ggcatcgcat ctattgcaac attaacctgg cagactgctg caagtccggg
     3181 aacactatat gatattgaag tggcaacaga ttcattattt acgaatgtta ttgcatctgc
     3241 taccggcata agtacaaata cttttaccac tactccattg ttatcaagta caacctatta
     3301 ttggcatgta aagtcttata attcatgttt tgcaaatagc tacagcgctg catggtcttt
     3361 cacaaccaac aattgctcga atttagcaag tgccaatgtt cctgttgtta ttccatcaag
     3421 tggtgcacca acagttactt caacacttaa catagcatct gctggaacaa taacagatgt
     3481 taatgttgtt ggtcttaatg gcacacacac ctggattagc gaccttacca taagcattaa
     3541 aagtccgcaa aacacaactg taacattgtt tgatcagatt tgtaccaatg aaaatgattt
     3601 tgactgtaat tttgatgatg cagctgcacc gggtgcatta ccttgccctc caattggtgg
     3661 cggcacttac aatgccgtta caccactgag tgttttcaac ggacagcaag cagcaggcaa
     3721 ctggacacta acagttaccg atcattttaa ttctgatggt ggctcattga atggctgggg
     3781 acttgaaatc tgcaccacta cgccaacagg aattgaaact cacatagttt ctacttccat
     3841 gattttatat cctaatcctg taaaagaatt gttgaacgta aatgttgcag gcaaaaaagg
     3901 agaacaagtt ttagtagaga ttaaaaacat tacaggacag gtgataaaat ctatctctgt
     3961 taatgcaaac actaaaattt ctattgatgt aaaagatctt tctgccggca tgtattttgt
     4021 tacagctaca ggcagtaata ttgaaacaca gcgcttcata aaagcaaagt aatttcccta
     4081 caatagctaa aagccttagt atccccgata ttaaggcttt tttaatgtct gaaaaacaac
     4141 ggaattattc ctttctaaac tattacattt gcccaataaa gaaatcgtat gcagataaac
     4201 ttcaagaagt taatccctca cggcatagcc atcagtattt ttttactctt aacagccgtt
     4261 tattttttac ctctattaga aggtaaggga ctgagtcaac atgacattgc tcaatggaca
     4321 ggtatgagca aagaacttac tgacttccgt gcaaaatatc atgttgaacc attatggaca
     4381 aatgctatgt tcagtggtat gcctgcctat cagatttcga ctttgtatcc ggctaatctg
     4441 atacaatatg taaatgactt attgtttttc tttcttcctt ctcctgcatc ctatgtgttt
     4501 cttgctctgt taagttttta cattctgatg ctggtgctga aatttgatta cagaatgggt
     4561 attgctggtg ccatagcttt tgcattttca agttataatt ttgtaatcat agccgcaggg
     4621 cataattcaa aggcacatgc cattgcctta atgccattgg tatttgcagg catcatcctt
     4681 accatgcgag ggagattttg gtttggtggt gctatgacag caatggcact tgcattacaa
     4741 ctttatgcca atcaccttca gataacatac tatctgatga ttgcaatttt agtaatggta
     4801 attgcctatt tcattgatgc catcatgaaa aaagagattg gtggttttat caaaagcgcc
     4861 tccgtactgg ccattgccgc agtgatagga attctgccaa actataccag tcttgctgca
     4921 acttatgagt atggaaaata ttcaacccgt ggccctagtg agcttacttc aaaaaaagaa
     4981 tcatcaggcc ttgataaaga ttatgcattt agctatagct atggtatcag cgaaacattt
     5041 actctcatca ttccacgttt tcatggtggt gcatctgtca gtccgttaga tgaaaattct
     5101 gctacataca aggcattaac aagtaatgga gtacccgttc aacaagcaaa aagttttatt
     5161 caggcagtgc cactctattt cggaaacatg ctttcaacct cgggcccacc ttatgccggt
     5221 gcaataattt gctttctttt tgttgcaggt ttaatgctga taaaaggaaa agaaaaatgg
     5281 tggctgctgg tagcaacaat tctttcattt atgctttcat ggggagaaaa ttttctttca
     5341 ttcaacgagt tattctttta tcattttccg ggatacaata agttcagagc tgtgagtatg
     5401 atacttacaa ttgcacagtt taccatgcct ttgcttgcac tccttgcatt aaaagaaata
     5461 tttgatggca aaacaaacaa gaacgatata ttaaagtcac ttaaaatggc attatatatt
     5521 accggtggat tttgtttgct atttacatta ttaccaggaa tgtttaatga ctttagctcg
     5581 caagctgatg aacaactgaa acaatatgac tggttaatta ctgccataaa acaagaccgc
     5641 gaaagtgcag tcagaatgga tgcattgcgc tcattatttt ttattgctgc aacttttggt
     5701 gtattatggt ttatgttaaa agataaaata aaggcaaccg ttgctataat aactattgct
     5761 gtccttttta ctgttgactt atggcctgta ggtaaacgtt atctcaatga caacaatttt
     5821 gttgcgaaaa gcaaaacaca aaaaccattt gagccttcac ctgctgatac acaaatttta
     5881 caggatacaa ctcccggatt cagagtgctt aacacaacac taaatgcatt cagtgatgca
     5941 tctacctcct attttcatcg cagcataggt ggctatcatg gcgcaaaatt aaaacgctat
     6001 caggaactga ttgaaaactg tatctccaaa aacaacatga gtgtgcttga catgcttaac
     6061 acgaaatatc tgatagtaag tgataaagaa caacagcctg tagcacagct taaccctggt
     6121 gcaattggtg ctgcctggtt tacagacact gtaaaaatgg ttgctaatgc cgatgaggaa
     6181 ataaatgcac ttaactcctt taatccacga acaattgcat ttgttgacaa gcgttttgaa
     6241 aatacattaa aatccttttc tcctcaacac gactctactg ctactgttaa acttaattct
     6301 attgtatgta atcaccttgt atatgaaacg cagacggcat tgccgcagtt ctgtgtgttt
     6361 tcagaaatct attatgacaa agggtggaac gcttacattg acggacaaaa agctgactac
     6421 gtgcgagcaa actacgtatt gcgtgccatg gtagtccctg caggaaaaca taaaatagag
     6481 tttaagtttg aacccgaagt ttattttaca ggagaaaaaa tttcacttgc agggtcaatt
     6541 gttcttctgt tgctctttgc tgcaggtgcc tggattgaat taaaatcagg aacagagaag
     6601 aaataaaagt ttattacatt gaaaaaagta ttaatcatca cctattactg gccacctagt
     6661 ggtggtgcag gagttcaacg atggttaaaa ttctcaaaat atcttcgtga ctttggctgg
     6721 gagcctataa tttttaccgt tgcaaatgga gaatttcccg aacaggacaa ctctctttta
     6781 aaagatattc cttcaaatat taaggtgatt aaacaaccta tttgggagcc ctatctactc
     6841 tataaatttt taacaggtaa aaagaagcac gataaaatac atgcaggatt tttaactgag
     6901 aaaaaaaatc cgggtatgct acaaaatctt tctgtttgga tcagaggaaa tctatttatt
     6961 cctgatgccc gatctttttg gattaaacca tccgtacgta ctttgacaaa atggttaaat
     7021 gaaaataaag tggatgctat tgtcagtaca ggtcctccgc atagctgcca ccttatagca
     7081 cttaaactga aaaggaaact caatttgcct tggcttgccg acttccgtga tccatggaca
     7141 aatattgatt tttataaaga cttaaaattg agttcctggg ctgatgcaaa acatcatcgt
     7201 ttagaaaaag aagtagttca aaaagctgat gctgttgtta ccattggcaa aacaatgcag
     7261 gaagaattca gcgcctcgtt taaaagaaat attcattgta taaccaatgg tttcgaccat
     7321 gatgacatct taaatacatc tgcaactgtg gacttaaaat tttctattgc tcacattgga
     7381 actatggtaa agacacgtaa tcctattgtg ttatggaaag cattgagtga attattaact
     7441 gaaattccta aattaaaagc agaccttgaa ataaaaattg tgggtaaagc agatttggaa
     7501 gtaagtcaat cgttagaaaa ttttgcacta actactttta taaacagaat tgattatatg
     7561 ccacatgagg aagtagttaa attacaacaa caatcacaag tacttctatt ggttgttaac
     7621 aatactccaa atgcaaaagg tgtgcttaca ggaaaaattt ttgaatatct tttagctaaa
     7681 cgtccaatcc tttgcatagg ccctgaagat ggtgatgcag ccgctattat taatcaaacc
     7741 caagccggat ttgttgcagg atttaatgat gtgaatcgca ccaaacaaat agtaaaagat
     7801 ttatataata aattcaaaga aaataatctt aacattcaat ctcaaaatat agatcagttc
     7861 agccgtaaaa atctgacaaa acaacttgct ttagtattga atgatattgc acatttatct
     7921 taacagtaca tttccattat aaacaactac atatttttgt tgcattaaaa tttaaaaata
     7981 tcagaaaata agggtcagta attatttcct acttttacta ctctaatcta tattgaaatt
     8041 tttttaatca ttccataatt ccaacttgtg ttcaaactat taaccatcat cggggcacgc
     8101 ccacaaataa taaaagctgc tgctatcagc agggcaatta aaaaatcttt tagtgataaa
     8161 attaaagagg ttattgtaca taccggacag cattacgatg aaaaaatgtc tgccatcttt
     8221 tttgatgagt tgaatattcc ccgtgaagac tacaatttaa aaaccggttc agggagtcat
     8281 gctgtacaaa ctgcggaaat catgcgtttg ttagaatcag ttcttgaaaa agaaaagccc
     8341 aatgccgttt tagtttatgg cgataccaac tcaacacttg caggtgcact gactgctgct
     8401 aaaatgcata ttcctgtggt tcatgtagaa gcaggcatgc gttcattcaa caaatcaatg
     8461 cccgaagaga ttaatagaat ttgctgtgat catgtttcaa cattactttt ttcaccaaca
     8521 aaaaccggtg taaacaatct ctgtcgcgaa ggcttttctg aacatacact tccaccattt
     8581 aacattaaca atccaaaagt atatttctgt ggtgatgtga tgtacgataa ctctctgtac
     8641 tttgctgcac aagctaaatc acaatcatct attctatcaa aatccgggat tgtaaagaat
     8701 gactttatgc tctgtaccat tcatcgtgac agcaacacgg ataacccaaa aaatctttca
     8761 gaaattttca gagcgctgat caaaatttct gaaacacaac aacaaaacat tgtaattcca
     8821 ctacatccaa gaacagccaa ggtgctggaa caaaacctga gcccggaatt gtttcagcaa
     8881 gtaaacaata gtactctttt aaaaattata gaacctgttt cttttattga tatgataagt
     8941 ctggaatctg aatgtacaat gattatcaca gattcgggag gtgttcaaaa agaatcctat
     9001 tttttccaaa aaccatgcat cattcttaga aatgaaacag aatggattga attagttgaa
     9061 gcaggtactg caatcattgc cggtccggat tatgctaaaa ttgttgatgc ttataaccaa
     9121 ctcgcttcat caaaattaag cttcgcacct ttatttggtg atggcaaggc atcagagttt
     9181 atatgcagtg aaatgctatc tgtattgaat tgactacatt tacatgaaat aaaaatgtca
     9241 tgaagtacat tttatcgaag tcaagttaca taaaaagttt acaatgtctt aagtcgcttt
     9301 acttctataa atttaactat aaagaccgtg atccgattag tgcagagcaa caagctaagt
     9361 ttgatcgcgg acatcaatat ggaaaaattg cacatcaact ttttcccgga ggtgtagatg
     9421 tttcaccgcc ttcgccattt gagtatgcaa catcagcaaa acaaacacat tcattattac
     9481 taaaacagca acctgtaatt tatgaagctg cctttattgc aaatgaagta attgttgcct
     9541 tggatattct ggtgcgtaaa ggcgggcttc tccatgcatt tgaggtaaaa agttctactg
     9601 ctatcagtca aacaatttta aatgatgcag ctttacaata ttatgttatc acacagtgtg
     9661 gatttgaact ggaagatttt agcatcatcc atctgaaacc tgattataaa gaacttcctg
     9721 ttgacgatgt aaaagcacta ttcgtttctg aaagtgtaaa agagtggtgt gtgtcacaac
     9781 aacaatttgt tgaagacaac attttagccg ctaaagaaat catccgtaac aaaaaaatgc
     9841 ccaataaaaa acagggcgaa cattgtaatt cgccctatcc ttgtgatttt aaaaatattt
     9901 gtaataagag taactccttc gattcaggaa gtttatttgg ataagttaat gcatcactgt
     9961 aaatcgtgca gtgtttgaat tctttttatc agacaatttt aaaatgtaaa caccattatc
    10021 caaatctcta acaacaattt gaatcttact accattagct tgaatttctt tcaccattct
    10081 tccttttaaa tcaaatactg ttagtttggt catttccaac ggtgacggca ttagcactgt
    10141 taaaatatca gaaacaggat ttggccaaac agaaacttct aactgattat tcaactcttt
    10201 cactcctaca aaaggggaag ctccaagatt aagccattca tttgcacctg tagtcgtagt
    10261 tccatcaaaa ccacctacaa caaccatata caaactatcg ttgcttacaa ctcctgtgaa
    10321 attacttaca ttactgcatg cagttggttt tggcggacct atcagccatt cgccattatt
    10381 caaatcataa gcccaaacgt catcttttac ttctgaaccg acacctgtag ggtcacctgc
    10441 agcaaatacc acgtagggca aactgttttt aaatgttcca ccacctgcaa atcgtgtcat
    10501 tgtgccaccg ggatatggcg ctgctgcctg ccatgtaatg tttgttgggt ttgcaggatc
    10561 tatctgacct atccatgttt catcaattgc accttgcaaa gtctgactgt atccacaagt
    10621 tacaacaata taattattat aaattcctcc tctaaaaccg gatacagcag tgcctggttt
    10681 aggcgttccc atactccaac tgtttgttgc cgggttgtat atctgaacag catttaaatc
    10741 actagttcca tcataaccgc caataaaata aattaatgaa tcgttgtaaa ctccacttac
    10801 atagtcaccc acaggctgtg gcattactgc acccgatgtc caactgttag tctgtgaatt
    10861 ataaatataa ttggtagcag aaggaacaaa gcccgtacta taaccaccaa ttgcatacat
    10921 catattttgt acaccatgca ttgaaaactg ataaacgcct aagggcatat cagtagcaga
    10981 agtccagcta tttgtaatct catcaaattt ttcattctta ggctgaatga tacttaaatc
    11041 acttccgcca tcagaaaaca aatatcctga atcaagaggg gcatttacag aaggaataaa
    11101 tgccgatgct ccaccgaagg ttgctattgt agcaggtgtt ttgcttgtcc atccatagtt
    11161 tggaagttca ttataagctg ttgttgtttt atttaaagtg tcgttagata aatcactgtc
    11221 agtggttaat tgagaataaa cggtaatgtt ataacttcct gttactcccg gagtccacgg
    11281 gtcaaagctt acctgttgtg tttgattgaa tgccagattg cttactgttt tagttgatgt
    11341 atatccacct gaaatatcca tagtaacatt aaaggtttga gaagtaagtc cgttattcag
    11401 aaccgttgcc ataggagtct gtgtacctgt tccaactgcg ctgcgaacat caatactctt
    11461 aaccatagca tcattggcag caattgtcat ggggcctgtc catttgtaaa tgggcgaatt
    11521 caattctcca cctccactcc aaccggtagt ggcatttacg aagtcgcaat ctgaatggat
    11581 gagattatcc atcaaacgcc agttaacacc attatcaatg ctgaatgctg aaccgtaaat
    11641 ggtataggga gaagtgatga caaaagtaga gtctgttcca ggtacataac gaattgattg
    11701 tttcatagta aaacctgcag atgaagtagg caaaagactc catgttgcac caccatcgtt
    11761 agtgacaatt aaatctgttg atgtggttgc attatcagcc tcacatgcaa ttccaaagtt
    11821 agcatcgcga aaagtgatgg caccgataaa acccgtataa ggggtactgc tcacagtcca
    11881 gttcattcct ctgtctgttg aattataaat gcgtccttta ttagttccga aatagacagt
    11941 actgtcgcca tagttggtat agacatcagt aataccaaat tcacccgtca ggtttgctgc
    12001 aatatttgcc tgaggcacac gaatccaatt gttgccacca tctgttgttg tgtaaatttc
    12061 aaaatatcca ccatttgggt cgcccataca aaagccttcg ttagcattaa aaaaatgcac
    12121 aacattagga aagccattag gcgctgtaaa agttgccgtt gtttgttttg tccaggaaac
    12181 accaccatca ttggttcgca aaatacttcc tcctccactg acattaaaca ttgctaccca
    12241 tgcagtatcg gcattgagag ccataataca tgacgggcca tgattggctg catttgtaat
    12301 agtacctgct gtccatgtca gtccgccatt ggtcgttcgt gtaaattcct gaactggatt
    12361 ctgctgcgaa ttgcggtcag aagataatcc ccatactaca ttggtatcaa ctgcatcaat
    12421 aaattgcaca tagttgtttg ggttgatgtt tgaggattgt gctatccatt gctgagcatt
    12481 taactgatta ctaaccgtca gacaaagaga aacccatgta aaaaagagta aagacttttt
    12541 cataaatttt tgattttagt ggtttgaaca gttttgattt atcaaacaaa tataataaaa
    12601 tcattaaacg ggctattaaa agcttttaca tctttatata tcggtcaaaa aaaaaccggc
    12661 cttacgaccg gtttactgta ttatgagttt tgattctctc tgggcattct tcgctcacga
    12721 ggtttgccat catttggctt acctgcatca cccggttgac gttccggctt tggcaacaac
    12781 actttacggg ataatttcag tttaccactt ttctcttcta ctccaatcaa tttcacttca
    12841 actaaatcgc cttctttaaa aacaccatcc atggttgcta atcgctcatg tgtaatttca
    12901 gaaatatgta acagaccatc tttaccgggc ataatttcaa caaatgctcc gaaggttgta
    12961 atattcttta cttttccttt ataaacggtt ccaatttcag gaactgcaac aatagctttt
    13021 acacgctcta atgctttgtc catgctctct tttcctacag cagcaatctg cacatagcct
    13081 ttattgttct tctcttcaat tgtgatagta gcaccacttt cacgttgaat ctcctgaata
    13141 atttttcctc cgggtccaat cactgcacct atctgatctg tttcaattgt aaactcaaca
    13201 atacgtggtg catttggttt gtaatcagga cgcgcttctg aaatagtttt ttccatttcg
    13261 ctgataatat gcaaacgtcc ctgacgtgcc tgctctaatg cctgcgccat tacttcataa
    13321 ggcaaacctg ttaccttcaa atccatttgt gttgcagtga taccgttgcg tgtacctgca
    13381 actttaaaat ccatatcacc taagtgatct tcatcaccaa gtatatcact caatatagca
    13441 tagcgtcctt tgccatcgct aatcaatccc attgcaatac ctgccactgt tgatttcatt
    13501 ttgatacctg catccatcaa tgccatactt cctgcacaca cagttgccat agaacttgac
    13561 ccgttagatt caagaatatc tgaaaccact ctgatggtat atggattttc tgattccggt
    13621 ggaagtgctt ttgttaatgc tctcaatgca aggttaccat gtccaacttc tctgcggcct
    13681 gttccgcgca ttggccttgc ctcgccagta gaaaatggag gaaagttata atgcaacaaa
    13741 aactttttag ttccttcaat catggcacca tcaattatct gctcttccat tttaccgcca
    13801 agcgtaacag aagtcaaaga ttgtgtttcg ccacgtgtaa atacagctga gccatgtgca
    13861 gttggtagat aatttgtttc actccaaatc ggacgaactt ctgttaaccc acggccatca
    13921 agacgttttt tctctgccaa taaagcttcg cgaactgcat catattccac ttcatgatag
    13981 taatgattta tgaatgctga cttttcttct aattcttcct ccgtaaaacg ggttttaaac
    14041 tcttctacga ttgcttcaaa tgattttttt ctttcgtttt tattaggatt accttgtaaa
    14101 gctacctgat aaactttctg ataagtttca tcacgaacca cttttctcaa atcttcgtca
    14161 tgctcttcgt gattgtattc acgcttggtt tttccaccaa ttttttcgag taaatccaac
    14221 tgtgccttgc actgaatttt aattgcttca tgcgctactt tgatagcttc aagcatggta
    14281 gcttcactta cttcactcat ctcaccttct accatcacaa tattttccat tgatccggca
    14341 acaatcaaat caagtgtggc acgtgcaatg tctgcaatat agggattgat aaccattgca
    14401 ccatcaattt gagctacacg aacttcggaa ataggtccgt taaatggaat atcacttatg
    14461 gttaatgcag cagatgctgc aaaggctgca agggcatcag gataaactgt actatcacct
    14521 gaatgtagtg ttactaatac ttgtgtgtcg gcatgaaaat tttcaggaaa cattggacgc
    14581 agtgcacggt caatgagtct gctgataaga atttcatatt ctgatggtcg cgcttcgcgc
    14641 ttaaaaaatc ctcccggaaa gcgaccaaca ctggcatatt tttcctgata gtcaaccgta
    14701 agtggcataa agtcaactcc tggcattgca tcttgcttgg cacatgcagt agctaaaagc
    14761 atacaatcgc ccattcttac tactactgaa ccatcagctt gtttggctag tttgcctgtt
    14821 tcgattgaaa tctctctacc atcgggtaaa gtaaatgtgt gtttaactcc taaattcatt
    14881 ttatcgtctt atttaaaaac aaaaaggcaa ttgtttacca ttgcctttct gaaatattat
    14941 tgtattcaat ttaaatttta cttccttaaa ccgaggtcag ctacaatttt acggtaacgt
    15001 gtaatatcgg ttttgtgaag atagttaagt aaacttcttc tttttccaac aagcttaatc
    15061 agcgattgtt gtgtagcgta atcttttggc tgaattttaa gatgatcagt caggtgattg
    15121 atgcgctcag aaaacatggc aatttgtgct tcagaattac cggtattttt atcgtttccg
    15181 ccaaactgct taaacagttc cttttttctt tcagatgtaa ctatcattgg tatagactat
    15241 gattttgtaa aataagtgtg caaagatagg tttttatttg attataatat tcaatttctc
    15301 aaaaaaaata aagccccata ctttgacagg gctttaattt attaattata ttgcaagtca
    15361 attagttaac taattaaaat ttataagaaa gacctatgcc gaaaacctct ttgaactgaa
    15421 gacgcggccc ggttttagtt tcttttactc cgcttttttc tttgataacc gttacaggaa
    15481 taatgtcatc ataaatcatt tgagttccaa tgttggcagt gatgaattta tttaccttca
    15541 tagcaatcag aatttcgtga tttacagcga tgttctgtgg attgtcaaga taattgctga
    15601 acaactccag tttggtttgg aagttcacat ttgtaaatac gtctttttta tacatggcac
    15661 gtaagtaagc acctaactga gttaatgatt ttttgcctgg gtcaacacca aaagcacctg
    15721 cctgagccaa catatcatca tttacaataa tagtgcggcc tgttaaagga gaaaggtata
    15781 aactaaatgc atcgttgggt ttgtaatcaa taccaattga ataaagcaaa tatccgggtg
    15841 ctaaaaattt cgagattgga tatttgagta aagtatcgtt cttataatca tatccgtttg
    15901 caaactgact tttgaagttg aagaggaagg tgtaatagaa cttactttca tgcgaaacct
    15961 tataaccaac ctttgaatta agctcaattt tatcttcgtt ttttcgaact ttagcatcgc
    16021 ctgattttaa taaaccataa gccaggtcaa gttgattatc ccatgccaaa cgatcttttg
    16081 catagttggc aaacagtgtc aaaaatgctg ctcctgacat tgagttttct ccgcctgctg
    16141 cccaattact taatgcaacc tgattgaagt taatagatcc cattccacct tttttccatg
    16201 atgtatcaac cggtgcagaa gtttgagcca tggtgctgcc agcaaatgca acagcaataa
    16261 cagttgtaat aatttttttc attgtaatga ttttaatatt taatttttaa tgcttttata
    16321 taaagcaaag gagggattgt tgtcaacccc tcccccctat ttataaattt tattttttca
    16381 gtaaatcgcg aatctcgcca agcaactcct gatcttttgt tggtccggcc ggtgctggtg
    16441 caggttcttc ctttttcaga ttgttcattg ctttgatgac taaaaacatg acaaatgcaa
    16501 tgattaaaaa gtttaaacac actgtaataa attcaccata cttgataaac acttcaggca
    16561 ctgcctttgt ggttatattt cctgctgcat caagtacttc cttgctgcca tcctgaattt
    16621 tatatttaag ttcgctaaag tcagcaccac ctactaattt acctacaata ggcattacta
    16681 tgccatcaac aaatgatgta gtaacttttc caaaggcacc gcctactaca acaccaatag
    16741 caaaatcaac tagattgcca cgcatggcaa aatctttaaa ctcttttaga aatcccataa
    16801 tatttagttt ttaaatgtta gtcagaaaaa tttcactgca aatgtatcat tccaatcttt
    16861 gaacaaaaca caatgcacta caaaaattca aaaaccttca caaaattttt agatttattc
    16921 aggaaacttc tgctgtaacc gaaatttcta cattaacacc tttgggcagt ccggcaacag
    16981 caacagtttc gcgagccgga aaattatctt taaaataact gccataaacc ttattaactt
    17041 catcaaaatc tgacatggaa gtcagaaaga tggtagtctt tacaatgttt gaaaaattta
    17101 atcctgctgc cagtagtata gcctcaatat tttgcatgat gcgcgtagtc tgagcagaaa
    17161 ttgatgaagt aatcagttgt ccgtttgcag gatctattgc aat
//
LOCUS       DAOOOL010000043        17127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_268_length_17127_cov_136.355143, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000043 DAOOOL010000000
VERSION     DAOOOL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17127
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_268_length_17127_cov_136.355143"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..667
                     /gene="pyrH"
                     /locus_tag="PKU82_05235"
     CDS             2..667
                     /gene="pyrH"
                     /locus_tag="PKU82_05235"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOZ82313.1"
                     /translation="MGEKNFGIDNNRLTQYAEEIKTIVDKGVQVAIVIGGGNIFRGLQ
                     AAEGGMDRVQGDYMGMLATVINSMALQGALEHKGINTRLQSAITMDAICEPFIRRRAV
                     RHLEKNRVVIFGAGTGNPYFTTDTAASLRAIEVEADVILKGTRVDGIYTSDPEKNSDA
                     VRFEHLTFDDVYEKDLKVMDMTAFTLCHENKLPIIVFDMNTPGNLLRLVEGNHVGTLV
                     DVE"
     gene            complement(773..1483)
                     /gene="truB"
                     /locus_tag="PKU82_05240"
     CDS             complement(773..1483)
                     /gene="truB"
                     /locus_tag="PKU82_05240"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOZ82314.1"
                     /translation="MPDYLQGEVLLVDKPLEWTSFDVVNKLRGTLQHLKFEDGSRPYK
                     KIKVGHAGTLDPLATGLLIVCTGKATKSIDSLMGLEKEYTGTFTLGQTTPSYDAETTV
                     DRTFDISHITTELIHQAAQTFVGETEQLPPAHSAVIIQGERAYEKARRGEEVKVTPRK
                     INIQSFEITAIAMPVVSFRVVCSKGTYIRSLANDFGKALNSGAYLSSLIRTCIGDFKL
                     SNAHSVEHYVEAIRSLQK"
     gene            complement(1489..2289)
                     /locus_tag="PKU82_05245"
     CDS             complement(1489..2289)
                     /locus_tag="PKU82_05245"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOZ82315.1"
                     /translation="MNTLQAIILAIIEGITEFLPVSSTGHMIIGSSVMGIAELPITKT
                     FTVAIQFGAILSVVFLYWRKFLVIDFNFYKKLLIAFIPAAVLGKLLDDYIDALLENVL
                     VVAAMLLVGGIIFILLDKWFQKTEERGSEELTKDKSAFYIGLFQCIAMIPGVSRSAAT
                     IIGGLAQGLNKKAAAEFSFFLAVPTMFAATLYKMYGFYKLNGGFTAEEIKLFAVGNVV
                     AFIVAAIAIKSFINYLTKHGFKIFGWYRIIIGAAILLLHFAGIELKML"
     gene            complement(2311..2589)
                     /locus_tag="PKU82_05250"
     CDS             complement(2311..2589)
                     /locus_tag="PKU82_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOZ82316.1"
                     /translation="MAKEKTITKSTTAKSASKPEIQLAFGKINYILMISGIVLILFGY
                     MLMSGGGSDDPKVFNPAIFDTQRLTVAPIMVVLGYALEVLAIVVNAKD"
     gene            complement(2602..3474)
                     /locus_tag="PKU82_05255"
     CDS             complement(2602..3474)
                     /locus_tag="PKU82_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOZ82317.1"
                     /translation="MSEDNKLARKALRSSNISTVISISLVLFLIGSLGLLLINAKQLS
                     DYLKENVILTVMMKQEASDADIQNLKKQLEGNAFIKEVSYISSEQAAEQLKQELGEDF
                     NTFLGFNPLLPLMEIKLKPDYTEENNISQLKDFISKNSIVKEVYFQESLLDAINKNIR
                     NISLIILGFSAVLFFIAGALINNTIRLALYAKRMLIKSMKLVGATRNFIRKPFIVRGI
                     LQGLIGAVIANIMILVVFYYAKKQIPEIIAFENPVNLSIVLGIVVFLGIFISGFSTYF
                     AVNKYLRQRTEDIF"
     gene            complement(3569..4567)
                     /locus_tag="PKU82_05260"
     CDS             complement(3569..4567)
                     /locus_tag="PKU82_05260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOZ82318.1"
                     /translation="MSRVLVIRFSSIGDIVLTTPVLRCIKKQLPNAEVHFLTKRDFSD
                     VLANNPYIDNTFYVDNNLPEIIRQLKMVGYDYIVDLHHNMRSWKVKRAVRAKKFSFNK
                     LNIEKWIYVNFKINKLPHKHIVDRYFDAISPMGVVNDGQGLDYFINPLDEVDISTFLP
                     ASFRQGYVAFVVGAKHATKCLPLEKMKEIISGLKHQVVLLGGFEDKQKGAELQKLFPE
                     KVFNACGITSIGQSASLIKQSKAVITHDTGLMHIAAALRKKIVSVWGNTVPEFGMYPY
                     LPVNSVPFSISEVKNLNCRPCSKIGYNTCPKKHFRCMNDMDIQEIIKNTQHYFETK"
     gene            4654..5418
                     /locus_tag="PKU82_05265"
     CDS             4654..5418
                     /locus_tag="PKU82_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOZ82319.1"
                     /translation="MIFTDTHTHLYATEFDADRKELMQQAIDAGVSRVFLANVEKSSV
                     NEMLELCKQFPENCFPMMGLHPCSVKEDYLAELALVESELSKGKYFGVGEIGLDFYWD
                     KSFIKEQEEAFLKQITWSCELNLPVSIHSRSATTRAIELIQLSGLSVKGVFHCFSGSI
                     EEAKAITDMGMYLGIGGVVTYKNSGLDAIVSQVGLKHIVLETDAPYLPPVPHRGKRNI
                     PEYLLLTAQKLAVIKNCSVAEVAEQTTNNSKVIFKQ"
     gene            5418..6440
                     /locus_tag="PKU82_05270"
     CDS             5418..6440
                     /locus_tag="PKU82_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOZ82320.1"
                     /translation="MKTDKKNLLVIYTGGTIGMIHDEETGSLKPFNFGKLSTVVPELK
                     KLPCKIDFHSFDPLIDSSDMQPERWIQIAEVIEENYGKYDGFIVLHGTDTMAYTASAL
                     SYMFQNLNKPVILTGSQLPIGEIRTDAKSNLITAAEIACGNVVVPEVCICFNNQLFRG
                     NRAEKFTSSKFDAFQSINYPSLAEAGTEINYNHDAIREVNNNALKVKTHFNNSVMIFK
                     LFPGVNEKVLSAILKSGALKGLIIETYGSGNAPKNEKLLKQLGDAVKNQMVIVNVSQC
                     SGGVVQMEKYENGLALKRAGVVSGHDMTTEAAITKLMFLLGSEMDYQEIKVNMQLDMA
                     GELSRE"
     gene            6632..6719
                     /locus_tag="PKU82_05275"
     tRNA            6632..6719
                     /locus_tag="PKU82_05275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6666..6668,aa:Ser,seq:gga)
     gene            6744..7364
                     /locus_tag="PKU82_05280"
     CDS             6744..7364
                     /locus_tag="PKU82_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HOZ82321.1"
                     /translation="MYTGILHTHTLVVSLFIVLYLIKATLLVLNRHAALDNFVKKTKV
                     AEMIISTLFLLTGIYLAVNTGNKGTWLWVKLFVIACTIPLAVIAFKKKNSSLALISLM
                     ALIYAYGISETKSISMKKADTAVNTEGLEGAAAGKVIFESKCQSCHGADGKLGLSGAK
                     DLTQSALSPQEKIDIITNGKKTMAAYKDQLSQEQIAAVAEYVQTLK"
     gene            7387..8592
                     /gene="hflX"
                     /locus_tag="PKU82_05285"
     CDS             7387..8592
                     /gene="hflX"
                     /locus_tag="PKU82_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOZ82322.1"
                     /translation="MKEKTVFKPLTDVKERAVLVGVILKDQPVEQEMEYLDELAFLAE
                     TAGAQTLKSFTQKLDHTDPRTFVGSGKLAEINEFIKANEINLAVFDDELSPSQQRNIE
                     KILQIKVLDRTQLILDIFARRARTAQAQVQVELAQYQYLMPRLTRMWTHLEKQQGGIG
                     NRGPGEKEIETDRRIVRDRLSKLRERLKEIEKQGFNQRKARGNMVRVALVGYTNVGKS
                     TIMNLLSKSDVFAENKLFATLDTTVRKVVLENIPFLLSDTVGFIRKLPHHLVESFKST
                     LDEVREADILIHVVDISHPNFEAQINVVNQMLQELQSADKPVILVFNKIDAFTYIEKE
                     ADDLTPMTSENISLDDLRKTWMNKGNVETIFISATEKDNISELRTMLLEKVKRIHLVR
                     YPNNKITND"
     gene            complement(8589..10526)
                     /locus_tag="PKU82_05290"
     CDS             complement(8589..10526)
                     /locus_tag="PKU82_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOZ82323.1"
                     /translation="MVARHDPLNKITLGGLLVTLGIIYGDIGTSPLYVMNAIIGDRPI
                     SPELVLGGISCIFWTLTLQTTFKYVFLTLRADNKGEGGIFSLYALVRKTKVKWLAIAA
                     ILGGSALLADGLITPAISVSSAVEGIQILHPDLPTIPIIITIIFGLFFIQQFGSNFVG
                     KSFGPMMLIWFSMLFVLGFHQLIQNPSVLKAFNPYYAYQLLVNYPGGFWVLGAVFLCT
                     TGAEALYSDLGHVGRKNIYITWTMVKFCLLINYFGQGAHLLKFEGKTIDVNPFYQLMP
                     DWFLLIGIIISTLAAIVASQALISGAFTVVNEAMRLNFGPKLKVVYPTDLRGQVYIPT
                     VNWTLCAGCIGVILFFQHSSNMEGAYGLAIIIDMMITTILLSFYLYLKRYPLPLVMLT
                     FLLFIVIEIAFLVANSIKFEHGGYVTMLLTMFLFITMYVWYAARKIRNQFVDFVKVKD
                     FLEILTNLSKDESIPKYATNLVYLTSANRRDEIESKVMYSLLQKFPKRADIYWFVHVD
                     VLDEPYTMEYKVAEMVNDKVIHIDFRLGFRIEPRINQMFRQVVEELVKNNEVDITSRY
                     TSLKEKNVIGDFRFVVIEKTLSYDNKLNFYEKFVLNAYDFLKLFSLPESKAFGLDTSS
                     VTVENVPLVVKPSGKFKLKRI"
     gene            complement(10626..10699)
                     /locus_tag="PKU82_05295"
     tRNA            complement(10626..10699)
                     /locus_tag="PKU82_05295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10663..10665),aa:Ile,seq:cat)
     gene            10780..11406
                     /locus_tag="PKU82_05300"
     CDS             10780..11406
                     /locus_tag="PKU82_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOZ82324.1"
                     /translation="MIKNIIFDFGGVLLPINTEKTVLEFGKMGLDNFDELYSMQKQAK
                     FFDDFEKGLIPASAFRSEIRKHYKRPLSDEEIDYAWNALLGELNPERFNFLSELRDKY
                     NIYLLSNTNIIHHAAFIKSLNQTFGKDRFEKLFEKTYYSFQMGTRKPDKRIFADVIKD
                     SGVTPAETVFIDDNLNNALGAQKVGMNAIHHDNAMEIEKELMAMLSAF"
     gene            complement(11403..11807)
                     /gene="mce"
                     /locus_tag="PKU82_05305"
     CDS             complement(11403..11807)
                     /gene="mce"
                     /locus_tag="PKU82_05305"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOZ82325.1"
                     /translation="MKNLEHIGIAVKSLQISDALFEALIGCPPYKKESVESENVITSF
                     FQTGETKVELLEATSPESAIAKFIDKRGEGIHHIAFAVDDIKAEMQRLQQSGFTLLNS
                     EPKKGADNKLVCFLHPKSTNGVLVELCQEIKS"
     gene            complement(11794..12489)
                     /locus_tag="PKU82_05310"
     CDS             complement(11794..12489)
                     /locus_tag="PKU82_05310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOZ82326.1"
                     /translation="MHPEVRKIFKSITVPLLLSLLMIGVKIFEVLSHTDLGEYGVLPR
                     QISGLKGILFIPFLHADWEHLFSNVVPLLVTGSMLFYFYKEMAVKVILLIYILSGFWL
                     WLGGRENYHIGASNIVYGITAFLFLSGFIRKHTGLIAVSLLMVFLYGSLIWGLFPVVP
                     KISWEGHLFGALSGILCAIVFRKEGQQKEVYEWNDDEPFDDQENDDNATQSSLKIVYS
                     YKKSDEKNGTEES"
     gene            complement(12644..13090)
                     /locus_tag="PKU82_05315"
     CDS             complement(12644..13090)
                     /locus_tag="PKU82_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ82327.1"
                     /translation="MINIRKGTPADVEPALRLIKELAEYEKAPEQVINTVEQMLKDGF
                     GEKPIFDLLVAETNDNIVGIAIYFIKYSTWKGKCLYLDDIVVQESLRGKGIGRKLFDA
                     VAAEAKLQNCQQLQWQVLNWNEPAINFYKKYDTVFDDEWINCKIIL"
     gene            complement(13094..14683)
                     /locus_tag="PKU82_05320"
     CDS             complement(13094..14683)
                     /locus_tag="PKU82_05320"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ82328.1"
                     /translation="MLEVKNLKVVFHQNSNPVVAVNDFSFSIAAGETLGLVGESGSGK
                     TVSSLALLGLLNNKNVAISGNASFSLQQPVDLLTLNEKQLTAIRGRHIAMIMQEPMQA
                     FNPLFTCGHQVTEAIRKHTALNKNDATEKVLQQFEKVKLPNPLQISKRFPHQLSGGQL
                     QRVAIAMAISCNPSLLIADEPTTALDATVQKDILLLLQQLQSENNMSMLFISHDLEAV
                     KLIAHNTLVVHAGQMVEQEKTEKIFAHPAQEYTKELLQSRPSGFKRNSVISEKNILSV
                     ENISKSYNQRQGLLRSKQNIIKAVDNISFTVKQDETLGIAGESGSGKTTLAKLLLRLI
                     ETDSGVIKLSEENLKDYPIRTKQLWRKKIQAVFQNPHGSLDPEMTVGRQLTEVFKLHF
                     SSQNTTTVKNQIIEWFEKVGLTEQHFNRYPHELSSGQKQRLCIARALIPQPDLLICDE
                     AVSALDVSVQAKILELINRLQQQFHFGCIFISHDLKVIRQMCDNVLVMKDGKADCYSA
                     TEKLFEKPPTTYSEILLKSII"
     gene            complement(14723..>17127)
                     /locus_tag="PKU82_05325"
     CDS             complement(14723..>17127)
                     /locus_tag="PKU82_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOZ82329.1"
                     /translation="DSLPVSRYAMITAKFFAMCLIVAMLQLTVMFMGIFIQTLKGFYR
                     YDIDVYLKTLFVSQYPSLILLVLLAFFIHSMVNNKFIGHTLMIAYFVGRMSAAAMGIT
                     HKMIWYGNIPSAPYSDMNGYGHFLYPKYMFLIYWGSLGLVLFIIGVMMNRHGSETGIK
                     ARWQIFKQQWKFSRGRKIVWSLLLVFIVSGCFVFYNTNILHKFKSDKTVRHEQAEYEK
                     RFRKYLDFNQPRITAVNVNIDLVPEKRYAAIKGYYIIQNKSALPIDTFLITWAHENIK
                     INRFGFDILADEVENYQKQFVVYKLAHALKPGDSTKVYFDIEYINKGFANSEEELGLV
                     NNGTFINNNYLPAFGYSESFELTDNSDRKKEDLPRKNHTMRLLSDSASRNNTYLANDA
                     DWIRYESIISTSPDQIAVSPGYLQKEWTQNGRRYFHYKMDAPMVNFYSFLSARYEVFR
                     DSFQGINIEIFYHKTHSWNIEKMNKAIKKTLAYCQKTFSPYQFRQVRILEFPRYETFA
                     QSFPNTIPFSEAIGFIYDVSEDTDIDNAFYVTSHEVAHQWWGHQVCGANSQGSTLMVE
                     SMAQYTALMVMEKEFGKDAMEKFLKYELDGYLSGRSSERINEQPIARNDGQGYIHYQK
                     GSLVMYAMKDYLGEDTLNAALHRFIKTYGFQSPPYVQAPQFVQYIADATPDSLKQVVE
                     DMFNRITLFDLKCVEAGYKKKGNKYEVTLDVETGKVYADSMGNGTGVKPNDWIDIGIM
                     KYDGNGKHSYQYLQKQLFTGGRKTIVVTVDEKPSKAGIDPLHKLIDRNSEDNIKEVKS
                     LQ"
BASE COUNT         5407 a         3171 c         3227 g         5322 t
ORIGIN      
        1 aatgggagag aaaaattttg ggatagacaa caataggctt acccaatatg cagaggagat
       61 aaaaacaatt gttgacaaag gtgttcaggt tgcaatagtt attggcggag gtaatatttt
      121 cagagggttg caggcagcag aaggcggcat ggacagggtg cagggcgatt acatgggtat
      181 gctggcaaca gtaattaata gtatggcttt gcaaggtgca ttggaacata aaggcataaa
      241 cacgcgtttg caatctgcca ttactatgga tgccatctgt gagcctttta taagaagaag
      301 ggcagtgcgc caccttgaaa aaaacagagt tgtgattttt ggtgcaggaa ctggtaatcc
      361 ctattttaca acagatacag cagcatcatt acgtgccata gaagtagaag ccgatgtaat
      421 tcttaaagga acaagggttg atggcatcta cacatccgat cctgagaaga atagcgatgc
      481 agtgcgcttt gaacatctga cctttgatga tgtttacgaa aaagatttaa aggttatgga
      541 tatgactgca tttacactgt gtcatgaaaa caaacttcct atcatagtct ttgacatgaa
      601 tacaccgggc aatctgttgc gacttgtaga aggcaatcat gtaggcacgc tggttgatgt
      661 ggaataaatt gcaagaagat ttttggtgaa tagaatttcg aaaagatttt tttgataatg
      721 aaatactaaa aggagaatga ttcatttcaa aactagccac cccctaaaaa aactacttct
      781 gcaaacttct tattgcttca acataatgtt caacactgtg tgcattgcta agtttaaaat
      841 caccaatgca tgtgcggatg agtgagctca gatatgcacc ggagtttaat gctttcccaa
      901 aatcgttggc taaagaacgt atgtaggttc ctttactgca tacaacacga aatgaaacaa
      961 caggcatagc tattgctgta atctcaaaac tttgtatatt aatttttcgt ggtgtaactt
     1021 ttacttcttc gccacgtctt gctttttcat aagcacgttc accctgaata attacggcag
     1081 aatgtgcagg tggcagttgc tctgtttctc caacaaaagt ttgtgctgcc tgatggatta
     1141 gctccgtagt gatgtgcgat atatcaaatg tcctgtcaac tgttgtctct gcatcatacg
     1201 atggtgtagt ttgtccaaga gtaaaagtgc ctgtatattc tttttcaaga cccattagtg
     1261 agtcaatact ttttgtggcc ttaccggtgc aaacaataag caaacccgtt gctagtgggt
     1321 ctaaagttcc ggcatgtcca acttttattt ttttataagg tctgctgccg tcttcaaatt
     1381 tcagatgctg taaggtgcct cttaatttat tcactacatc aaaagatgtc cactctaaag
     1441 gtttatctac caacagcact tcaccttgta aataatcagg catgcagatt aaagcatttt
     1501 aagttcaatg cctgcaaagt gcaacagcaa aatagcagca ccaataatta ttctgtacca
     1561 tccgaaaatt ttaaatccat gcttggtcag ataatttata aatgatttta tggcaatggc
     1621 agctacaata aatgcaacaa catttcccac agcaaataat tttatttctt ctgcggtgaa
     1681 acctccattc agtttataga agccatacat tttatatagt gtagctgcaa acatagttgg
     1741 cacggcaagg aagaacgaaa attctgcagc agctttttta ttgagtccct gagctaagcc
     1801 gccaatgatg gtagctgccg agcgcgatac tcccggaatc atggcaatgc actgaaacaa
     1861 tcctatataa aaagcagatt tatcttttgt gagttcttcg cttccgcgtt cttctgtttt
     1921 ctgaaaccat ttgtcaagca aaatgaatat gatacctcca acaagcaaca tggctgcaac
     1981 caccaataca ttttcgagca aagcatcaat gtagtcgtca agcagcttgc ctaaaacagc
     2041 agcaggaata aatgcaatca gtaacttttt ataaaaatta aaatcaataa ctaaaaactt
     2101 acgccagtat aaaaatacca cagataaaat tgcgccaaac tgaatggcaa cagtaaatgt
     2161 tttagtgatg ggaagttcgg caatgcccat tacggaggag ccgataatca tgtgtccggt
     2221 agaagataca ggcagaaact ctgtgatacc ttcaatgatg gccagaatga tagcctgcag
     2281 tgtattcatt aaaatagttg gaagagtgtt ttaatctttt gcattcacaa caatggcaag
     2341 tacttcaagg gcatagccaa gcactaccat tattggcgca actgttaatc gttgtgtatc
     2401 aaaaatggca ggattaaaaa cttttggatc gtcagaaccg ccaccactca tcagcatata
     2461 gccgaacaga ataagtacta tgcccgaaat catcaggatg taatttattt ttccaaaggc
     2521 taattgtatt tccggctttg atgcagactt tgctgttgta cttttggtta tggttttttc
     2581 ctttgccatg aatttgaatt gttaaaagat gtcttcagtt cgttggcgaa gatatttatt
     2641 tactgcaaaa taggtgctga atcctgaaat aaaaatgcct aaaaacacca caatgcctaa
     2701 aactatgctc aaattcaccg gattttcaaa tgctatgatt tcaggaatct gctttttggc
     2761 atagtaaaac actactaaaa tcataatatt ggcaatgact gcgccaatca gaccttgcaa
     2821 aattcctctt acaataaatg gttttctgat aaaatttctt gtggccccaa ctaacttcat
     2881 actttttatg agcatacgtt tagcatagag cgcaagcctg atagtgttgt tgattaatgc
     2941 accagcaata aagaacaaca cagcactgaa tccaagaata atcaagctga tatttctgat
     3001 atttttattg atggcatcga gcaacgactc ctgaaaataa acttccttaa ctatactgtt
     3061 tttagaaata aaatctttta gctgcgaaat attattctct tcagtatagt cgggcttaag
     3121 ttttatttcc atcagtggca acaatggatt aaaccctaag aaagtattga aatcttctcc
     3181 cagttcttgt tttaattgct ctgccgcctg ttcgctgctg atgtatgata cttctttaat
     3241 aaaagcattt ccttctaatt gttttttaag attctgaatg tcggcatcag aggcttcctg
     3301 cttcatcatc accgttaaga ttacattttc ttttaagtaa tcagaaagtt gtttggcatt
     3361 gatgagtaac agccccaatg aaccaatcag aaacaatact aatgaaatac taattacggt
     3421 tgaaatattt gaactgcgaa gtgccttcct tgccagttta ttatcttcac tcatggcagc
     3481 aaatttagat ggtaaaacac tgcatcaata acctgaaaca gaatttattc aacagggtta
     3541 ttaataaatt ttaattatcg gcatcggatt atttggtttc aaaataatgc tgtgtgttct
     3601 ttatgatttc ctgaatatcc atatcgttca tacagcggaa atgtttttta ggacaggtgt
     3661 tgtaaccaat tttagaacaa ggcctgcagt ttaaattctt cacttcgctg atgctgaaag
     3721 gcacactgtt tacaggcaga tagggataca ttccaaactc agggacggtg tttccccaaa
     3781 cggaaacaat tttttttcgc aaggctgcag caatatgcat cagtccggtg tcatgtgtga
     3841 ttacggcttt actttgtttg atgagtgatg ccgactggcc aatacttgtt atgccgcagg
     3901 cattaaaaac tttttcggga aataatttct gaagctctgc tcctttctgt ttgtcttcaa
     3961 atccgccaag caaaacaacc tgatgcttca gtccgctgat aatttctttc attttttcaa
     4021 gtggcagaca tttagtagca tgtttggcac ccacaacaaa ggccacatat ccctgacgga
     4081 atgatgcagg taaaaaagta gaaatgtcaa cttcatcaag cgggttaatg aaatagtcaa
     4141 gaccctgccc atcattcacc acacccattg ggctgatggc atcaaaataa cggtcaacaa
     4201 tatgtttatg cggcaactta tttattttaa aattgacgta aatccacttt tcaatattaa
     4261 gcttgttaaa actgaatttt tttgctctta ctgctctttt cactttccat gaacgcatgt
     4321 tgtgatgcag gtcaacaata taatcatagc caaccatttt tagctgcctg ataatttcag
     4381 gaaggttatt gtcaacataa aaagtattgt caatgtaggg gttgtttgcc aatacatcac
     4441 taaaatcgcg cttggtaaga aaatgcactt ctgcattggg caattgtttt ttaatacaac
     4501 gcaaaacagg tgttgttaaa acaatatcgc caatagagct aaaacggatg actaaaactc
     4561 gggacataca gtggttttcg aaaaaatgtc taatgccatt gtttgcaaag gtaagcattg
     4621 gtttaaatac ttaaattcga tttactttgc atcatgattt ttacagatac acacacacat
     4681 ctctatgcca ctgagtttga tgcagacagg aaagagctta tgcaacaggc tattgatgcc
     4741 ggtgtgagtc gtgtttttct tgctaatgtt gaaaaatcaa gtgttaacga aatgcttgaa
     4801 ttgtgcaaac agtttccgga gaattgtttt cctatgatgg ggttgcatcc atgctctgta
     4861 aaagaagatt atttggctga gttggctttg gttgagtctg aattgtcaaa gggaaaatat
     4921 tttggtgtag gagaaattgg tcttgacttt tactgggata aaagttttat aaaagaacag
     4981 gaagaagctt ttttaaaaca gattacatgg tcttgtgaat taaatctgcc ggtaagcatt
     5041 cattcgcgct cagcaactac acgtgccatt gaacttatac aattatcagg tctaagtgta
     5101 aaaggtgttt tccattgttt cagtggaagt atagaagaag caaaagctat aaccgacatg
     5161 ggaatgtatc tgggcattgg aggtgtagtt acttacaaga attcaggtct tgatgcaatt
     5221 gtcagtcagg taggattaaa gcacattgtt ttggagaccg atgcgccata tttacctccg
     5281 gtgcctcatc gtggcaaaag gaatattcct gaatatttac tgctgacagc acagaaatta
     5341 gctgtcataa aaaattgttc ggtagcagaa gttgctgaac agactacaaa caattctaaa
     5401 gttattttca aacaatgata aagacagata agaagaacct tctggtaatt tataccggtg
     5461 gaaccatagg tatgattcat gacgaagaga caggatcgtt aaaacccttt aacttcggca
     5521 agctcagcac cgttgtacct gagttgaaga aacttccctg taaaatagac tttcattcat
     5581 tcgacccgct gattgacagc tctgatatgc aacctgaacg atggatacaa attgcagaag
     5641 ttattgaaga gaattatggc aagtatgatg gatttattgt tttacatgga accgatacta
     5701 tggcatatac tgcttcggca ttgagctaca tgtttcagaa tctgaacaag cctgtaatcc
     5761 tcaccggatc acaattacct attggcgaaa tcagaacaga tgcaaaatca aaccttatta
     5821 cagcagctga aattgcctgt ggaaatgttg tggtgccgga ggtttgtatt tgttttaaca
     5881 atcagttgtt cagaggtaac agagcagaaa aatttacatc ttcaaaattc gatgcttttc
     5941 aatccatcaa ctatccgtca ttggctgagg caggcactga aattaattac aaccacgatg
     6001 ccattcgtga ggttaacaac aatgcgctga aagtaaagac gcacttcaat aattcggtga
     6061 tgatttttaa attgtttccg ggagtaaatg aaaaagtatt gtcagcaatt ttaaagtcag
     6121 gtgcgctaaa aggattgatc attgaaacgt atggctccgg caatgcgcct aagaatgaaa
     6181 aactgctaaa gcaattaggc gatgcagtaa aaaatcaaat ggtaattgta aatgtttcgc
     6241 agtgtagtgg aggcgttgtg cagatggaaa agtatgagaa tggacttgcg ttaaaacgtg
     6301 caggtgtagt cagtggccat gacatgacta ctgaagctgc cattaccaaa cttatgtttt
     6361 tattgggcag cgaaatggat tatcaagaga taaaagtgaa tatgcaattg gatatggcag
     6421 gagaacttag tcgtgaataa atgtgttgat aacaaactac gcattggcgc agttgttcaa
     6481 tttgtcaacc tacttttgat gcggttttaa tcaatctgtt gacaacggtc aaaagatagg
     6541 tttggtttta ggttctcagc ctgtcgtatt tttttcggca ggctttttct ttagccatca
     6601 tgttaatttg ttagctttgc aggctcaaaa aggagaggtg tccgagtggt tgaaggagca
     6661 cgcctggaaa gtgtgtttac gccaaaagtg taacgagggt tcgaatccct ctctctccgc
     6721 aaaaatcatt tatctttatc attatgtaca caggaatttt acacacacac acactggtag
     6781 tttcactttt tattgtttta tacctcatta aagcaacctt gttagtgctg aacaggcatg
     6841 ctgcattaga caactttgtt aaaaaaacta aggttgcaga aatgatcatc agcacattgt
     6901 tcctactaac cggaatttat ctggctgtca acacaggtaa caagggtaca tggttgtggg
     6961 ttaaattgtt tgtcattgcc tgcactattc cacttgcagt aattgcattt aaaaagaaaa
     7021 acagctcatt ggcattgatt tcactcatgg cattgattta tgcttatggc atttctgaaa
     7081 ccaaatcaat atccatgaaa aaggctgata cagctgtaaa tacagaaggc cttgaaggtg
     7141 ctgctgcagg taaggttatt tttgaaagta aatgccagtc ttgtcatggt gctgatggaa
     7201 aattaggatt gagtggtgca aaagacctta ctcagtctgc tttatctcca caggaaaaaa
     7261 ttgacatcat taccaatggc aaaaaaacaa tggcagccta taaagatcaa ttatcacaag
     7321 aacaaattgc tgccgttgca gaatatgttc agacactgaa ataatttatt tcagttttat
     7381 ttattgatga aagaaaaaac cgttttcaag ccacttactg atgttaagga gcgtgccgtt
     7441 ttagtaggcg taatattaaa agatcagcct gtagaacagg agatggagta tcttgatgag
     7501 ttggcttttc ttgcagagac tgcaggcgca cagaccttaa aatcgtttac acaaaagtta
     7561 gatcataccg acccacgcac ctttgtaggt tcaggaaaac tagcagagat aaatgagttt
     7621 atcaaagcga atgaaataaa ccttgctgtt tttgatgatg agttgtcgcc ttcgcagcaa
     7681 cgcaacattg aaaaaatatt acagataaag gtattggaca gaacacaact tatcctcgat
     7741 atttttgcac gaagggcacg cacagcgcag gcacaggtgc aggttgagct cgcacaatac
     7801 caatatctga tgccacgcct gacacgcatg tggacacacc tcgaaaaaca gcagggaggt
     7861 ataggtaacc gcggtcccgg tgaaaaagaa attgaaacag acagacgtat tgttcgtgac
     7921 cggcttagta aactccgtga acgtttaaaa gaaatagaga agcaaggctt taatcaacgt
     7981 aaggcaagag gtaatatggt gcgtgttgca ttggtaggtt atactaatgt gggcaagtcc
     8041 acaattatga atctgcttag caagtcagat gtgtttgcag aaaacaaact ctttgcaaca
     8101 ttagatacta cagtcagaaa agttgtgctt gaaaatattc cttttttgtt gagcgatact
     8161 gttggattta tcagaaagct gccacatcat ttagttgaat cattcaagtc aacacttgac
     8221 gaggtgcgcg aagccgatat tttaattcat gtagtggata tttcgcatcc caattttgaa
     8281 gcacagataa atgtggtgaa tcagatgttg caggaacttc aatcagcaga caaacctgta
     8341 attcttgtct ttaataaaat tgatgcattt acttatattg aaaaagaagc agatgacctg
     8401 acacccatga caagcgaaaa tatttcatta gatgatttac gaaagacgtg gatgaataaa
     8461 ggcaatgtag aaacaatatt tatttcagct acagagaaag ataatatttc tgaactgcga
     8521 accatgttac tcgaaaaagt aaaacgtatt cacttggtgc gttatcccaa caataaaatt
     8581 acaaacgatt aaattcgttt gagtttaaat ttacctgaag gttttactac taaaggaacg
     8641 ttttctacag ttacagaact ggtgtcaaga ccaaaggctt ttgactcggg caaactaaat
     8701 aattttaaaa agtcataagc attgagcaca aacttttcat agaaattcag tttgttgtca
     8761 tacgatagtg ttttctcaat cactacaaaa cggaaatcac ctatcacatt tttttcttta
     8821 agtgaagtgt agcgactggt aatatcaacc tcattatttt ttacaagctc ttcaacaacc
     8881 tgacggaaca tctgattgat tcgtggctct attctgaaac caagtctgaa atcaatatgg
     8941 ataaccttat catttaccat ttctgcaacc ttatattcca tggtgtaagg ttcatcaagc
     9001 acatcaacgt gaacaaacca atagatgtcg gcacgcttcg gaaatttctg taagagcgaa
     9061 tacatcactt tcgattcaat ttcatctctg cggttggcac tggtaagata cacaaggttg
     9121 gtagcatatt taggaataga ttcgtcttta ctcaggttgg taagtatctc taaaaaatct
     9181 tttaccttaa caaagtcaac aaactgattt ctgattttac gtgctgcata ccaaacatac
     9241 atggtgatga ataaaaacat agtaagtagc atggtaacat atccgccatg ttcaaattta
     9301 atggagttgg ccaccagaaa agcaatttca atgacaataa aaagaagaaa tgtaagcatt
     9361 actaacggta gcggataacg ttttaaatac aaatagaatg aaagcagaat tgttgtaatc
     9421 atcatatcaa tgattatcgc taatccataa gcaccttcca tattggatga gtgttgaaaa
     9481 aatagtatta cgccaataca tcctgcacat aaagtccagt taacggttgg aatatatact
     9541 tgccctctga ggtctgttgg ataaacaact ttaagttttg ggccaaagtt cagtcgcatg
     9601 gcttcgttta ctacggtaaa tgcaccactg attaatgcct ggctggcaac aattgctgca
     9661 agagtagaaa taatgatgcc tattaacaga aaccaatcag gcataagttg gtaaaatgga
     9721 tttacatcaa ttgtctttcc ttcaaacttt agcaggtgtg caccctgacc aaaataattg
     9781 atcaacaagc aaaatttcac cattgtccag gtaatataaa tatttttacg tcctacgtga
     9841 ccaaggtcag agtaaagtgc ttctgctcct gtagtacaaa gaaaaactgc accaaggacc
     9901 caaaagcctc caggataatt tacaagtaac tgataggcat agtaagggtt gaaagcctta
     9961 agtacagacg ggttctgaat gagttgatga aatcctaaaa caaataacat ggaaaaccaa
    10021 atcagcatca taggaccaaa agattttcca acaaagttgc tgccaaactg ttgaataaaa
    10081 aacaaaccaa agattatggt aataataata ggtattgtag gtaggtctgg atgaagaatc
    10141 tgaatacctt ctacagcaga cgaaacagag atggcagggg tgattaatcc atcagcaagt
    10201 aaggcgctgc ctccaagtat tgcagcaata gccaaccact ttaccttagt ttttctaact
    10261 aatgcataga gtgaaaaaat tccaccttca cctttgttgt cggcacgcag tgtcaggaaa
    10321 acatatttaa aagttgtttg aagtgtcaat gtccagaata tacaactgat acctccaagt
    10381 actaattcag gacttatggg tctgtcgcca atgatggcat tcattacata gagaggagat
    10441 gtacctatgt cgccatagat aattcccaag gtgactaaaa gtcctccaag tgttattttg
    10501 tttaacggat cgtggcgtgc aaccatatta taaaaatgat gacaaaggta ttattcttga
    10561 aacttaactt cagaaaggaa aaaagaattt ttaataaagt gtggttttta tcgagctaaa
    10621 gaaagtgggc ccacctgggc tcgaaccagg gacccacaga ttatgagtct gttgctctaa
    10681 ccgactgagc tatgggcccg aaaagaatta taagttaaat gaactttgag ggctgcaaaa
    10741 gtatattttt gcacgcaata aacaaaagat agcaagagaa tgattaaaaa tatcattttt
    10801 gattttggtg gggtgttact tccgataaat acagaaaaaa ccgttttgga gtttggaaaa
    10861 atggggttgg acaattttga cgaactgtat tcaatgcaaa agcaagccaa gttttttgat
    10921 gatttcgaaa agggacttat ccctgcctct gcatttcgca gcgaaataag aaagcattat
    10981 aaacgtccgc tttctgatga agaaattgat tatgcatgga atgccttgct tggtgaactg
    11041 aatcccgaga gatttaattt tctgagtgag cttcgtgata agtataatat ttaccttttg
    11101 agtaatacca acattattca tcatgctgca tttatcaaga gtcttaatca gacttttgga
    11161 aaagaccgct ttgagaaact gttcgaaaaa acatattatt cttttcagat gggaacacgc
    11221 aagcctgata aaagaatttt tgctgatgtg ataaaagaca gtggtgtaac tcctgctgaa
    11281 accgttttta ttgatgataa tctgaacaat gcattaggtg cacaaaaggt aggtatgaat
    11341 gccattcatc atgacaatgc catggaaatt gaaaaagaac tgatggcaat gctctctgct
    11401 ttttaagact taatttcctg acacaattct accaacacac cattagttga tttagggtga
    11461 aggaaacaaa ccaatttatt gtcggcacct ttcttgggct cactgtttaa aagtgtaaag
    11521 cctgattgtt gcagtcgttg catttctgct ttaatatcat caacagcaaa ggctatgtgg
    11581 tgaattcctt caccacgttt atcaataaat tttgcaatgg cactttccgg tgaagttgct
    11641 tctaaaagtt caacttttgt ttcgcccgtc tgaaagaatg aggtaattac attttcgctt
    11701 tcaacgcttt cttttttata aggcggacaa cctattaatg cttcaaataa agcatcagaa
    11761 atttgcagac ttttaactgc aataccaatg tgttcaagat tcttcagtgc cattcttttc
    11821 gtctgatttt ttatacgaat aaacaatttt taatgatgac tgtgttgcat tatcatcatt
    11881 ttcttggtca tcaaagggtt catcatcgtt ccactcataa acttccttct gctgaccttc
    11941 ttttctgaaa actattgcgc aaagtattcc ggacagggca ccaaataaat gcccttccca
    12001 tgaaattttt ggaaccacag gaaaaagccc ccaaattaaa ctgccgtata aaaaaaccat
    12061 gagcaatgaa acagcaataa gccctgtatg ctttctgata aaaccactta gaaataaaaa
    12121 tgctgtgatg ccataaacaa tattgctggc accaatatga taattttctc gtccaccaag
    12181 ccaaagccaa aagccactta aaatgtaaat gagcagtata acctttacag ccatttcttt
    12241 ataaaagtaa aataacatac tgcctgtaac taacagcgga acaacattac tgaacaaatg
    12301 ttcccagtcg gcatgaagaa atggaataaa caaaatgcct tttaatccgc ttatttgtct
    12361 tggcaaaaca ccgtactcac ctaaatcagt atgcgataag acttcaaaaa tttttacacc
    12421 tatcattaga agtgatagga ggagtggtac tgtaatgctt ttaaaaattt tacggacttc
    12481 cggatgcatg tgttttacct tacaaacaat ctgccattgt ggttaaacaa aaacaatctg
    12541 cctttattgc tgatgctttt cctggtttgg cagtgaaata ctgatttaat gacaatgtgt
    12601 tttgtgcaat tgaatttgtc tgtacgaatg taacaaagaa agactacaga ataattttac
    12661 aattgatcca ttcatcatca aaaacagtat cgtacttttt ataaaaatta attgcaggct
    12721 cgttccagtt cagtacctgc cattgcaact gttggcagtt ttgtaatttg gcttctgctg
    12781 caacagcatc aaacaatttc cggcctatac cttttccccg taatgattct tgcacaacaa
    12841 tatcatctaa ataaagacac tttcctttcc atgttgaata tttgataaag taaattgcta
    12901 tacccactat attgtcattg gtttctgcaa ctaacaaatc aaaaatcggt ttttctccaa
    12961 agccatcttt aagcatttgc tcaacagtat tgataacctg ttctggcgct ttttcatatt
    13021 ctgccaattc tttgataaga cgaagtgcag gttcaacatc tgcaggtgtt cctttgcgta
    13081 tgttaatcat tttttaaata atagatttta ataatatctc tgaataggtt gtaggaggtt
    13141 tctcaaatag tttttcagtt gctgaataac agtcggcttt gccatctttc atcaccaaaa
    13201 cattgtcaca catctgacgg ataactttca agtcatgcga aataaaaata catccgaaat
    13261 gaaattgctg ttgcagcctg tttatcaatt caagaatttt tgcctgaaca gaaacatcaa
    13321 gtgcactcac agcttcatcg cagataagta aatcgggttg tggtatcaga gcacgggcaa
    13381 tgcacagtct ttgcttttgt ccggaactta attcatgtgg atagcggtta aaatgttgtt
    13441 ctgttaatcc tactttttca aaccattcaa taatctggtt tttaacagta gtggtatttt
    13501 gtgatgaaaa atgtaattta aagacttctg taagttgtct gcccacagtc atctccggat
    13561 cgagtgagcc atgtggattt tgaaatactg cctgaatttt ttttcgccat aattgtttgg
    13621 tgcgaatagg gtagtcttta agattttctt cgcttaattt tatcacgcca ctatcagttt
    13681 caataagtcg cagtaataat ttagctaatg ttgtttttcc agatcccgat tcaccggcaa
    13741 tgcctaaagt ctcatcttgc tttactgtaa aactgatatt gtcaacagct tttataatat
    13801 tttgtttact gcgtaatagc ccttgccgtt ggttgtaact tttgctgatg ttttctactg
    13861 acagaatatt cttttcactg ataacactgt ttcttttgaa tcctgaagga cggctttgca
    13921 atagctcttt tgtatattct tgtgcaggat gtgcaaatat tttttctgtc ttttcctgct
    13981 caaccatctg tcctgcatga accactaatg tgttatgtgc aatcaatttt actgcttcaa
    14041 gatcgtgact gataaaaagc atgctcatgt tattttctga ctgtaattgt tgtagcaata
    14101 aaagaatgtc tttttgcaca gtggcgtcta aggctgttgt tggttcatca gcaattagaa
    14161 gtgatggatt gcaacttata gccattgcaa tggcaactct ctgtagctgc cctcctgaaa
    14221 gttgatgagg aaaacgctta cttatttgta atggatttgg tagctttact ttttcgaact
    14281 gttgcaatac tttttctgtt gcatcatttt tgtttaatgc agtgtgtttt cgaatagctt
    14341 cagtcacctg atgcccacaa gtaaacagag gattaaatgc ctgcataggc tcttgcatta
    14401 tcattgcaat atgacgacct ctgatagctg ttaattgttt ttcgtttagc gttaaaagat
    14461 caacaggttg ttgtaaactg aaagatgcat tgccacttat agcaacattt ttattgttca
    14521 acaatccaag taatgccaat gacgaaacag ttttcccgga acctgattcg ccaaccaatc
    14581 ctaaggtttc gcctgctgca atagaaaaag aaaaatcatt tacagcaaca acaggattac
    14641 tgttttgatg aaatacaact ttcagatttt ttacctcaag catggctgcc aagataaaaa
    14701 gtaaatgtca atgtcagaaa aattattgta aggattttac ttccttgatg ttatcttcgc
    14761 tattcctgtc aatcagttta tgcaatgggt caatacctgc cttagacggt ttttcatcaa
    14821 cagtaacaac aattgttttt ctgccacctg taaataattg tttctgcaaa tattgataag
    14881 agtgttttcc gttgccatca tatttcatta tgcctatatc aatccagtca tttggtttaa
    14941 cccctgttcc atttcccata ctgtctgcat aaacttttcc ggtctctaca tctaaggtaa
    15001 cctcatattt gttgcctttc tttttgtagc cggcttcaac acattttaaa tcaaacagcg
    15061 tgatacggtt aaacatatct tcaaccactt gctttaaact gtcaggtgtt gcatctgcaa
    15121 tgtattgaac aaactgcggt gcttgtacat aaggtgggga ctgaaatcca tacgttttta
    15181 taaatctgtg taaggctgca tttagtgtgt cttcgcctaa atagtctttc attgcataca
    15241 ttaccaaact acctttttga tagtgaatat atccctgtcc atcgttgcgt gcaataggtt
    15301 gctcatttat tctttctgat gaacgtcctg ataaatagcc atccaattcg tatttaagaa
    15361 atttttccat ggcatctttt ccaaactctt tttccatcac catcagtgct gtgtattgcg
    15421 ccatactttc taccatcaaa gttgaaccct gactgttggc accacagacc tgatgtcccc
    15481 accactgatg tgccacctca tgtgaggtta cataaaaggc attgtctata tcggtgtctt
    15541 cgctaacatc ataaataaaa ccaatggctt cgctgaatgg aatggtgttt gggaacgatt
    15601 gagcaaaggt ttcatagcgt ggaaactcca gaatacgaac ctgacggaac tgataaggtg
    15661 aaaaagtttt ctgacagtaa gccaatgttt ttttaatggc tttgttcatt ttttcaatgt
    15721 tccacgaatg tgttttgtga tagaatattt caatgtttat tccctgaaag gaatcacgaa
    15781 agacttcata gcgtgcactt aaaaaagaat aaaaattcac cattggtgca tccattttat
    15841 aatggaaata acgtcttccg ttttgtgtcc attctttttg aaggtatccc ggtgaaacgg
    15901 caatctgatc gggtgatgtg ctgatgatgc tctcatatct tatccagtct gcatcattag
    15961 ctaaataagt gttgttgcgc gatgcactat cactgagcaa acgcatggtg tgattttttc
    16021 ttggcaaatc ttctttcttt ctgtcgctgt tgtcggtcaa ctcaaacgat tctgaatatc
    16081 caaatgcagg caaatagttg ttattgataa aagtaccgtt gtttaccaac cctaactcct
    16141 cttctgaatt ggcaaagcct ttgttgatgt attcaatgtc gaaataaact tttgtagagt
    16201 caccgggctt taatgcatga gctaatttat aaacgacaaa ctgtttctga taattttcaa
    16261 cttcatcagc cagaatatca aaaccaaacc tgttgatttt tatattctca tgtgcccagg
    16321 ttattaaaaa tgtatcaata ggcagtgcag atttgttttg tatgatataa taacctttaa
    16381 tggcagcgta tctcttttca ggaaccaaat caatatttac attcactgct gttatgcgcg
    16441 gctgattaaa atcaagatat tttctaaatc gtttttcgta ttcggcctgc tcatgtctga
    16501 cagttttatc gcttttaaat ttatgaagga tgttggtatt gtaaaagaca aaacatcccg
    16561 aaacaatgaa aactaaaagt aatgaccata caatttttct tcctctgctg aatttccatt
    16621 gttgtttaaa aatttgccac cgcgctttaa ttcctgtttc actaccatga cgattcatca
    16681 ttacaccaat aatgaaaagt acaagtccta agcttcccca ataaattaag aacatatatt
    16741 taggataaag aaaatgtcca tatccattca tgtctgagta gggtgcagaa ggaatgttac
    16801 cataccaaat cattttatgc gttatcccca tagcggccgc tgacattctt cctacaaaat
    16861 aggcaatcat caaggtgtgg ccgataaatt tattgttgac catgctgtgg atgaaaaatg
    16921 caagcaacac aagtaaaatc aaacttgggt actgactgac aaaaagtgtt tttaaataaa
    16981 catcaatatc atagcgataa aatcctttta aggtctgaat gaaaattccc ataaacatga
    17041 cagtcaattg caacatggcc acaataagac acatggcaaa aaacttggct gttatcattg
    17101 catatcttga aacaggaagt gaatctg
//
LOCUS       DAOOOL010000044        16910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_276_length_16910_cov_130.723465, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000044 DAOOOL010000000
VERSION     DAOOOL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_276_length_16910_cov_130.723465"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1400)
                     /locus_tag="PKU82_05330"
     CDS             complement(102..1400)
                     /locus_tag="PKU82_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82330.1"
                     /translation="MRKIFISILFIISAGITNAQIDNLSNMSPEWIRTGGRNAAIKGS
                     DLVVYNPATLTRLEKGLHIGLGNQMFMRKPSHTYDLGWGEVKHEQDGNDYFVPNLFLS
                     YNKNKWAVWGGVYISGGGATANYPEGSLNTELIGLSLLSAFQGAYLGTGQQYLKASSL
                     YLTGTAGFSYAINEQFSAALGVRYVNATNKTKAGTELIGDFSNLPVNMEQEDNANGIA
                     AVISLSARPIEKLNIAVRLETNTKLNFKTKQKVDDLEQSTGGMAELVVDGQESRRDLP
                     GLAGIGIGYEFSKAFRLTAEMNYYFQQHADWGKRADGKRINTLAGDAMNFGGGFEWMW
                     SKYITWSAGAVYTMLDFNDMAGYYTTLGAFETAPYNNLTANTGFAIDLSEKFRVNVGV
                     AKAFYKKDKTVAIEAMAPNVVKAKLNNDVLMLGAGIDLRF"
     gene            complement(1495..2079)
                     /locus_tag="PKU82_05335"
     CDS             complement(1495..2079)
                     /locus_tag="PKU82_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HOZ82331.1"
                     /translation="MKLSIDLTYNTTQAWVDAVMNDFDSFLQDHADCERKASAMAMSF
                     VSKCPDKTKIIPELIETALEELKHFQQVYHIMEKRGIQLKAEMPEDLYIKQLLALLRS
                     KSSERLMDRLLLASIIECRGTERFRLVEEALQDNELKRFYKMLWASEAKHGNIFVKMA
                     LEYYPKDDVYKRLHELTVKEGEICKALLIKAALH"
     gene            2095..3366
                     /locus_tag="PKU82_05340"
     CDS             2095..3366
                     /locus_tag="PKU82_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82332.1"
                     /translation="MYKSNFPLNKPHTYFEKQQFVPLLQEPPHSQLQCIVVIPCFNEP
                     DLITTLDSLWKCDETKFPVEIIAVINSGRNADSNVMDCNNLTLKTMQLWMQKHNSNHR
                     HYQVIHCSDLPPKKAGVGLARKIGMDEAAYRFSLNGNDNGIIVCLDADCTVQPNYLVE
                     IEKHFSNNIKTAGCSVYFEHPLEGKQEPSIYDAIINYELYLRYYVGALRYCGFHAPYH
                     TIGSSMAVTNKAYQKQGGMNQRKAGEDFYFLHKIFHLGNFSELNTTTVYPSPRISNRV
                     PFGTGSAISKIVTAGNNNYPVYAFETFEILKVFFDQIKVNATSREMINIDSFAPALKN
                     FLSAHNAASKMIEIATQTINSKTYLQRFAHWFDGFMVLKCVHYMRDHFLEQEEVLVAT
                     KKLMNNLNISPGNLTKREMLLRLRKHDRSGS"
     gene            3387..4826
                     /locus_tag="PKU82_05345"
     CDS             3387..4826
                     /locus_tag="PKU82_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOZ82333.1"
                     /translation="MKLLNYINGQMVEPISQKWMDNTDPSTNTVFSQIPDSDERDVQM
                     AVNAAKQAFVGWSTMPVKKRSDLLLRIAALIEKNLQRFAEAESLDNGKPVWLAKQVDI
                     PRASANFHFYATAALHLAAETHAMENTAINYTLRQPIGIAGCISPWNLPLYLFTWKIA
                     PALASGNCVVAKPSEITPVTAFLLSQFCIEAGLPAGVLNIIHGTGPRVGSAIVSHPDI
                     PVISFTGGTKTGAEISRIAAPMFKKLSLELGGKNPNIIFEDCDFDHAVSTSIKSSFSN
                     QGEICLCGSRIFIQRSIYDKFKEAFISKVKGLKTGDPKDESNFLGAIVSQPHFEKILS
                     YIDIAKQEGGVILTGGKAIQPEGRCKDGFFIEPTVIENLPYNCRTNQEEIFGPVVTLT
                     PFDTEEEVLMMANSTNYGLAATVWTTNLTKAHKVAAALHSGIVWINCWLLRDLRTPFG
                     GVKQSGIGREGGFEALKFFTEEKNICIKL"
     gene            4848..5198
                     /locus_tag="PKU82_05350"
     CDS             4848..5198
                     /locus_tag="PKU82_05350"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOZ82334.1"
                     /translation="MFITVLKSKIHRAKVTQAELHYVGSITLDENLMDAADLIENEQV
                     QVLNINNGERFVTYVIKGERNSGVVCLNGPAARRVQLGDIVIVASYAMMEKEEAKNFK
                     PVLVYPDEKNQPVK"
     gene            5212..6225
                     /locus_tag="PKU82_05355"
     CDS             5212..6225
                     /locus_tag="PKU82_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOZ82335.1"
                     /translation="MKKKVVSVIKYALFLAAGVGMLWLTFRNQDFTQVIQKIENVHIG
                     WLLASVAAAILAHISRAIRWNMLITPLGYKPKLINTFWALCAGYFANLAVPRLGEITR
                     CGMLTKAEQVPFNELFGTVIVERIMDVLMLFLLLLAVATLEFHLIGGFLYDNIAAPLM
                     SKFQNLNLPLVIGVLVVGIIAVFAYFYFSKHSGKSKAIFEKINLLLKGVADGIKSIAK
                     MKNKSLFIFHTLFIWVMYFFASYLCFFALDSTSMLDAKAGLFILVIGGLGMSAPVQGG
                     IGAFHFIVSRGLMLYGISETEGIVYATIVHGYQTILVIALGAIGFFVLIATGKRIKAN
                     NGL"
     gene            6215..6703
                     /gene="rfaE2"
                     /locus_tag="PKU82_05360"
     CDS             6215..6703
                     /gene="rfaE2"
                     /locus_tag="PKU82_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOZ82336.1"
                     /translation="MDYNEIISKKIFNEQSLLQQLSIWRLQNQSLVFTNGCFDILHLG
                     HTQYLSQAKNYGDKLILGLNSDKSGACKKGLGRPLNNENARAFVLASLHVVDAVVLFD
                     EETPYNLIKLVQPDVLVKGADYEIEKIVGYDIVIQRGGKVITIDLTQGYSTTALLNRI
                     KQ"
     gene            6771..7190
                     /locus_tag="PKU82_05365"
     CDS             6771..7190
                     /locus_tag="PKU82_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82337.1"
                     /translation="MKQAAIKIPIVALSLLLLMGCESNHFFKSEKKLKSDIVGTWNRV
                     LFGQQNEETWIFVDGAIKIYQPNRHGDTADVGTFSIDATLASPYLKIENTSYLNQNPG
                     GDTYNHKWSIVKLDNKVLEIATDSPLGGVAQIEFIKQ"
     gene            7226..8590
                     /gene="radA"
                     /locus_tag="PKU82_05370"
     CDS             7226..8590
                     /gene="radA"
                     /locus_tag="PKU82_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOZ82338.1"
                     /translation="MAKVKSVFVCQNCGAQSPKWMGKCASCGEWNSYVEELITSGNSS
                     AQVNYKRSSKPEKITEIELHQEQRIVLPDHELNRVLGGGLVRGSLILLGGEPGIGKST
                     LLLQLAVRLKNLKTVYVSGEESGQQIRMRAERLNSDLHSQCYLLTETSLEIILQHLNA
                     LKPDMVIIDSIQTMFHSQIESSPGSVSQIRGCATELLRFAKDSGCPVFLVGHITKEGV
                     IAGPKVLEHIVDTVLQFEGDRHHIYRLLRSHKNRFGSTAEIGIYEMQGNGLAEVSDPS
                     QIMLSQRDEALSGIAIACNLEGMRTMLIETQALVSSAVYGVPQRSTTGFDQRRLNMLL
                     AVLEKRCGFKLGAKDVFLNITGGLKVDDPAIDLAVICAILSSNNDIPLEPLTCFTGEV
                     GLSGETRPVSRIEQRIKEAEKLGFKKIYFPAQNLAAFDKAMFKNIKLYPVERVDEVFS
                     LLFG"
     gene            8672..9682
                     /locus_tag="PKU82_05375"
     CDS             8672..9682
                     /locus_tag="PKU82_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82339.1"
                     /translation="MKTLISAVIAMVSFTISASAQSNIEIGMSGGATNYFGDLGNNEV
                     LQSTSTRPGGAITIRNFLNSPAKTGYQYQPLSVEARFSWHRIGYDETKSIGDKKGYQL
                     RNYGRGLSFRTDVYGVSTHVSYTLYANKRKPLYAQGAAMYFYAGVGIYYARPKADLFN
                     GSIDINNRYYFWSDGTIRDQDEKTGQGNIIKRDGKYETNLDEWRTEGQGYNSELGQKK
                     PYNLMHIGFPLGMGWRWGISKNIILSAELSYIKFITDYLDDVSDRYATYEEIKANFPE
                     EHKQDLAKYISDPSGKGTNGFEGVATSQRGNHRKTDGYAFLSLEVAYRLDFNWGKIKS
                     SF"
     gene            9737..10765
                     /locus_tag="PKU82_05380"
     CDS             9737..10765
                     /locus_tag="PKU82_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOZ82340.1"
                     /translation="MQLTTIPFQLRFRKPFVLASGTRTHTSVVYIKAIQNEITGWGEA
                     AMPPYVKETQESVIHFVQQLHIPIINTLDDLNLFFEKLYQTSGNYFAKAAINGAMLDW
                     WTRKNSLSLSEYFGIDKNLSTPDCCYTITNGDDIKHSIKQAVDFKLLKIKAGFDGDVE
                     FIKEIAAISEKQICIDANQGWVNVNVALQKTAQLKNLNIAFIEQPLPVDDWQGMQKLK
                     QHTTFNFIADESFQTIDDLKKIMDCFHGVNIKLMKCGGVSNAFEIAKSARQLNAKLLL
                     GCMSESSCGIATAGAIQQLFDWVDLDGPLLISNNPFTGVRYQEGKIFIENNVGTGALS
                     VESTLQAL"
     gene            complement(10746..11249)
                     /locus_tag="PKU82_05385"
     CDS             complement(10746..11249)
                     /locus_tag="PKU82_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOZ82341.1"
                     /translation="MIEIRSATADDIPFITEVYNDAILNTTATFDTEVKSIEDRMLWF
                     KNHDSSHPIIVAEDNGVLCGWASLTAWSDRCAYNGTAEVSVYVHKDHRGKGIGKALLE
                     ILILKGESKGLHYLLSRIASGNEASIHLHTIFGFRHIGVMKEVGFKFGNYVDVHLMEL
                     VFKEPAK"
     gene            11571..11741
                     /locus_tag="PKU82_05390"
     CDS             11571..11741
                     /locus_tag="PKU82_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82342.1"
                     /translation="MGINVTPKDLTNKEFMLKNQSAIQHAQAILKERQTESTRITFKA
                     APQTNIFKQSKN"
     gene            12102..12272
                     /locus_tag="PKU82_05395"
     CDS             12102..12272
                     /locus_tag="PKU82_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82343.1"
                     /translation="MGINVTPKDLTNKEFMLKNQSAIQHAQAILKERQTESTRITFKA
                     APQTNIFKQSKN"
     gene            12861..13037
                     /locus_tag="PKU82_05400"
     CDS             12861..13037
                     /locus_tag="PKU82_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82344.1"
                     /translation="MDLLISILLYLGVYATPADLQNKEFVAKNSEQINKAEMLSKDPN
                     FKCDGIVIVGSGNL"
     gene            complement(13081..14583)
                     /locus_tag="PKU82_05405"
     CDS             complement(13081..14583)
                     /locus_tag="PKU82_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82345.1"
                     /translation="MEFSEIKLLINFFAKHRHDFLGVPDVYFADKNYPELLWFYKEIS
                     KGSINNDQEAAEKLLQSTATNPAYQQLKAELEDRLVNLVFGLDPEKLMNSMLGRSSFR
                     AYIYFGAAMILRQQNASAFFSDHFFKKAADYATFTNDGMIGCLTDLMLADRMAIKNKP
                     DDFYHYRNRFKKSIKQFELEAELILMQRELDIFQVASNISQYKNAAKAKRYYLRAKKI
                     HQQLKTFNSNFYYGNIAFIYGYFSSNFELVEEALNEREAFYQNHPEYYSDFQKSFIAL
                     SRMLAYIHLRQYEKGKEEARIFLGKLSANSSDWLHALEHYFILCMHSGNFEQALNIYY
                     EATRSEYFINSNQEVKERWHYFEPYLNFILPGHFPKESINLLGFLDEISYYTTHKEDN
                     NIIIMIGQIIVMIEMGDYDKLYDRTSYLESYIKKYVDKRIYPRTYIFLNMLLTLFKNN
                     FQKTKTQKQTAVSLEKLKPVAGNKFFNTEGLEVIPYEKLWAAISEKLKRH"
     gene            complement(14694..15413)
                     /gene="rnc"
                     /locus_tag="PKU82_05410"
     CDS             complement(14694..15413)
                     /gene="rnc"
                     /locus_tag="PKU82_05410"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOZ82346.1"
                     /translation="MRFYGFFPINILFSADRKTYFSLHNILGFYPIKLSLYKLAFSHR
                     SQTKEVTNGIKHSNERLEYLGDAVLGAVVAEMLFKKFPFREEGFLTEMRSKIVSRENL
                     KHLAFKLGINHFIKKTHGPDSFRNIYGDALEALIGAIYIDRGYGKARQFILERMINHH
                     VDLESLEATEINFKSRILNWSQRTKNTVAFETVEDEKSNDKLIRVRLMINGEEVSTGM
                     DFVKKKAEQIAAERACTKLEI"
     gene            complement(15420..16673)
                     /gene="fabF"
                     /locus_tag="PKU82_05415"
     CDS             complement(15420..16673)
                     /gene="fabF"
                     /locus_tag="PKU82_05415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOZ82347.1"
                     /translation="MELKRVVVTGLGALTPLGNSVSEYWDGLLNGVSGACQITKFDAS
                     KFKTHFACEVKNFDPLVYLEKKEARKLDPFSQYAIAVSDQGVKDAGLDTANINHDRVG
                     VIWGSGIGGLRTFLDEVIAYAKGDGTPRFNPFFIPKMIADIASGHISMRFGYRGPNYT
                     TVSACASSTNALIDAFTYLRLGKADIIISGGSEASVNEAGIGGFNAMHALSERNDSPQ
                     TASRPFDLDRDGFVLGEGAGCIVLEELSHAQARGAKIYAEMVGGGMSADAYHLTATHP
                     EGLGAFNVMKSSLDDAGMTSADIDYINVHGTSTPVGDPSEIKAILKLFGDDAFKLNIS
                     STKSMTGHLLGAAGAIEAIASILAVQNNIVPPTINHFTDDPTFDPRLNFTFHKPQQRN
                     IRAALSNTFGFGGHNASVIFKKFTP"
     gene            complement(16685..>16910)
                     /locus_tag="PKU82_05420"
     CDS             complement(16685..>16910)
                     /locus_tag="PKU82_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOZ82348.1"
                     /translation="ASRVKAIIVDKLGVDEGEVTNEASFTNDLGADSLDTVELIMEFE
                     KEFNIAIPDDQAEKISTVGQAVDYITANVK"
BASE COUNT         5332 a         3106 c         3178 g         5294 t
ORIGIN      
        1 aacaacgaag acatcatgcg ctctgaaaat aaagaactaa acgattttgt ttttgcatca
       61 cgatggatgc aggctgttaa aaagcacttt taataaatcc gtcagaatct caaatcaata
      121 cctgcaccaa gcatcaacac atcattgttt agttttgctt taaccacatt aggtgccata
      181 gcttcaatag ctaccgtttt atctttctta taaaaggcct ttgccacacc tacgttcact
      241 ctgaattttt ctgataagtc aattgcaaaa cctgtgtttg cagtaaggtt gttgtagggt
      301 gctgtttcaa aagctcctaa tgtagtgtaa taacctgcca tatcattaaa atcgagcatg
      361 gtataaactg cacctgcact ccaggtaata tactttgacc acatccactc aaaaccacca
      421 ccaaaattca tagcatcgcc tgcaagtgtg tttattcttt ttccatcggc acgttttccc
      481 cagtcggcat gttgctgaaa gtagtaattc atttctgctg tcagacgaaa tgctttactg
      541 aattcgtatc ctataccaat accagccaaa cccggtaaat cgcgtctgct ttcttgtccg
      601 tcaactacca gctctgccat gccaccggtt gactgttcaa ggtcatcaac tttttgtttg
      661 gttttaaaat ttagcttggt gtttgtttcc agtcttacag caatatttaa tttctctatg
      721 ggtcgcgcac ttaagctgat aactgcggca ataccgttag cgttatcttc ttgctccata
      781 tttacgggca ggttactgaa atcgcctatc aactctgtgc ctgctttagt tttgttggtg
      841 gcatttacat agcgtacacc caatgctgca gaaaactgct cgtttatagc gtaactgaac
      901 cctgctgttc cagtaaggta aagagaagat gctttaagat attgctgtcc tgttccaagg
      961 taggcacctt gaaaagcact gagaagtgac agtccaataa gttctgtgtt taaagagcct
     1021 tcaggataat tggcagtggc acctccaccg gaaatatata caccacccca cacagcccat
     1081 ttatttttgt tgtatgataa aaataagtta ggtacaaaat aatcattacc atcctgttcg
     1141 tgttttactt caccccaacc taaatcatag gtgtgagaag gtttgcgcat aaacatctga
     1201 ttgcccaaac caatatgtag tcctttttcc aaacgtgtta atgtagcggg attataaaca
     1261 acaagatcac tgcctttgat tgcagcattt ctgcctcctg tacgaatcca ctccgggctc
     1321 atgttgctca aattatcaat ctgtgcattt gttattcctg cactaataat aaatagaatg
     1381 ctgataaata ttttcctcat actctgattt tttgtttgag taaaaatatt ttgcatttgc
     1441 attgtcattc catgcagaaa agaaactaat aaacaatttg tttttaataa gtgattaatg
     1501 caatgctgct tttatgagta gtgctttaca tatttcaccc tcttttacag tgagttcatg
     1561 cagacgttta tatacatcat ctttcggata atattcaagc gccattttta caaaaatatt
     1621 tccgtgtttg gcttcacttg cccacaacat cttgtaaaat ctttttaatt cattatcttg
     1681 tagtgcttct tctaccagtc tgaaccgttc tgttccacga cattcaataa ttgaggctaa
     1741 gagaagtctg tccatgagtc tttctgatga tttactgcgt aacaatgcaa gcagttgttt
     1801 gatgtaaaga tcttcaggca tctctgcttt tagctgtatg cctctttttt ccataatatg
     1861 atacacttgt tgaaagtgtt tcaattcttc caatgcagtt tcaatcagtt caggaataat
     1921 ttttgttttg tcggggcatt ttgacacaaa gctcattgcc atagcagagg cttttctttc
     1981 gcaatcggca tgatcctgaa gaaaagaatc aaaatcattc atcactgcat caacccaagc
     2041 ttgcgtagtg ttataagtta aatcaataga aagtttcatt tcgtttttaa atatttgtac
     2101 aaaagtaatt ttccgttgaa taagccacat acctatttcg aaaagcaaca gtttgttcct
     2161 ttactgcagg agcctccaca ttcacagctc caatgcattg tagttattcc ttgttttaat
     2221 gaacctgatt taattacaac gcttgattcg ctgtggaaat gtgatgaaac aaaatttcct
     2281 gttgaaatta ttgctgttat caactcagga agaaatgctg attccaatgt tatggattgt
     2341 aataacctta cactaaagac aatgcaactt tggatgcaaa aacacaattc aaatcacagg
     2401 cactatcaag ttattcattg tagcgacttg cctcctaaaa aagcgggtgt cgggcttgca
     2461 cgtaaaattg gtatggacga agcagcatac cgttttagtt taaatggtaa tgataatgga
     2521 ataattgttt gtttggatgc cgattgtaca gtgcagccaa attatttggt tgagattgaa
     2581 aaacatttca gcaacaatat aaaaactgca ggctgttctg tttattttga acatcctctg
     2641 gaaggaaaac aggagccttc aatttatgat gctatcatca attacgaatt gtatctgaga
     2701 tattatgttg gtgcattgcg ctattgtggt tttcacgctc catatcacac cattggctca
     2761 agtatggctg taacaaataa agcctatcag aaacagggag gaatgaatca gcgaaaagcc
     2821 ggagaagatt tttattttct gcataaaata tttcatctcg gaaatttttc tgagcttaat
     2881 acaacaaccg tttatccttc accgcgcatc agtaacagag ttccatttgg tacaggcagt
     2941 gccatttcaa aaatagtaac agccggtaat aataattatc ctgtttatgc atttgaaact
     3001 tttgaaattc ttaaagtatt ttttgatcag ataaaagtaa atgcaacatc aagagaaatg
     3061 ataaatattg attcatttgc tcctgctttg aagaatttct tatcggcaca caatgcagca
     3121 tcaaagatga tagaaatagc aacacagaca atcaacagca aaacctatct gcaacggttt
     3181 gctcattggt ttgacggttt tatggtttta aaatgcgttc attatatgcg tgatcatttt
     3241 ctggagcaag aggaagtttt ggttgccaca aaaaaattga tgaacaattt gaatataagt
     3301 cccggtaatt tgactaaacg tgaaatgttg ttaaggttgc gtaagcacga ccgttcaggt
     3361 agttgagaag tgcctatctt cgtgtcatga aattgttgaa ttacattaac gggcagatgg
     3421 tagaacccat cagtcagaaa tggatggata atacagaccc atctaccaac acagtttttt
     3481 cacaaatacc cgacagcgat gaacgtgatg ttcagatggc agtaaatgca gcaaagcagg
     3541 cctttgtggg atggagtact atgcctgtaa aaaaacgctc tgatttattg ctgagaatag
     3601 cagcactgat tgaaaaaaac ctgcaacgat ttgccgaagc cgaaagcctc gataatggca
     3661 aacctgtttg gcttgccaaa caagtggaca ttccacgtgc atctgcaaat tttcattttt
     3721 atgctactgc tgcattgcat cttgctgccg aaacacatgc catggaaaac acggcaatta
     3781 attatacttt acgtcagcca ataggtattg caggttgtat ttcaccctgg aacttgcctt
     3841 tgtatttatt tacctggaaa attgcacctg cattagcatc tggaaactgt gtggtggcaa
     3901 agcccagcga aattactccg gtaacagcat ttttactaag tcagttttgc attgaagccg
     3961 gacttccagc cggtgtttta aatattattc acggtacagg tccacgtgtg ggcagcgcta
     4021 tagtttctca tcctgatatt cctgtaatta gttttacagg cggcaccaag actggtgcag
     4081 aaatttcaag aattgccgcg ccaatgttta aaaaactttc tcttgaatta ggaggcaaaa
     4141 atcccaatat catttttgaa gattgcgatt ttgatcatgc tgtttctact tcaataaaat
     4201 cttctttcag caatcagggc gaaatttgcc tttgcggctc acgcatattt attcaacgca
     4261 gcatttatga taaattcaaa gaagcattta tcagcaaagt taaaggactg aaaacaggcg
     4321 acccaaaaga tgagtcaaat tttctgggag ctatagtatc tcaaccacat tttgaaaaaa
     4381 tactttcata tattgatatt gccaagcagg aagggggtgt aattttaacc ggaggcaagg
     4441 ccatacaacc tgaaggacgc tgtaaagatg gtttctttat tgagccaacc gtaatcgaaa
     4501 atctgcctta taactgtcgc accaatcagg aagagatttt tggacccgtt gtgacactaa
     4561 ctcctttcga cacagaagaa gaagttttaa tgatggctaa tagcacaaat tatggattgg
     4621 ctgcaacagt ttggacaacc aatctgacaa aggcacataa agtggcagca gcccttcata
     4681 gtggtattgt atggataaac tgctggcttt tacgtgattt gcggacacct tttggaggtg
     4741 taaaacaaag cggtattggc cgtgaaggtg gttttgaagc attaaagttc tttaccgaag
     4801 aaaaaaacat ctgcattaaa ctttaaccta ttacttttgc cgccactatg tttataactg
     4861 tattaaaatc gaaaattcat cgggctaagg tcacacaggc cgaattacat tatgtgggaa
     4921 gtattaccct tgatgaaaac ctgatggatg ccgctgacct gattgaaaac gaacaggtgc
     4981 aggtgcttaa tattaataat ggtgaaagat ttgttaccta tgttatcaaa ggagaacgca
     5041 acagtggtgt tgtttgcctc aatggtcctg cagcacgcag ggtgcagtta ggcgacatcg
     5101 ttattgtagc ttcttatgcc atgatggaaa aagaagaagc caaaaatttt aaacctgtct
     5161 tggtttatcc tgatgaaaaa aatcaacctg taaaataaat cccgacatag gttgaagaaa
     5221 aaagttgttt cagtaataaa gtatgcattg tttctggctg ccggtgttgg tatgctatgg
     5281 cttactttcc gtaatcagga ttttacacag gtaattcaga aaattgaaaa tgtacatatc
     5341 ggttggctgc tggcatctgt tgctgcagca atacttgcac atattagtcg tgccatcaga
     5401 tggaatatgc tcattacacc tttaggttat aagccaaaac tgattaatac tttctgggca
     5461 ttatgtgcag gatattttgc caatctggct gtaccacgtc tgggcgaaat cactcgctgt
     5521 ggcatgctca ccaaagccga gcaagtacct tttaacgaac tgttcggaac ggtgattgta
     5581 gaacgtatca tggatgtact gatgttgttt ttgttattgc ttgctgttgc aacattagag
     5641 tttcatctca ttggtggttt tttatatgat aatattgctg cacctttgat gagtaaattt
     5701 caaaacctaa atcttccatt agtaataggt gttttagttg ttggcatcat tgcagttttt
     5761 gcttatttct atttctcaaa acattcagga aagagcaaag ccattttcga aaaaataaat
     5821 ttattgctta aaggtgttgc tgatggcatt aagtcaatag caaaaatgaa aaacaaatca
     5881 ttgtttattt tccacaccct atttatttgg gtgatgtatt tttttgcttc ctatctgtgt
     5941 ttctttgcat tggattccac atctatgctc gatgcaaaag caggtttgtt tattcttgtc
     6001 ataggtggat tgggaatgtc tgcacctgta cagggcggaa ttggggcttt tcacttcatc
     6061 gtttcacgcg gactgatgct ttatggtatt tctgaaacag aaggaattgt ttatgcaacc
     6121 attgttcatg gctatcaaac cattttagtg atagctttgg gtgctatagg attctttgta
     6181 ttgatagcaa caggaaaaag aattaaagcc aacaatggat tataacgaga taatcagcaa
     6241 gaaaattttt aatgagcaat cgttattgca acaacttagc atttggcgct tgcaaaatca
     6301 aagtcttgtt tttaccaatg gttgttttga cattcttcac ttggggcata cacaatattt
     6361 atctcaggca aaaaactatg gcgataaatt aattcttggt ctcaattccg ataaatcagg
     6421 tgcttgtaaa aaaggtctgg gcagaccgct gaataatgaa aatgcacgag cctttgttct
     6481 tgcatcgcta catgttgttg atgcagttgt attatttgat gaggaaacac cctataatct
     6541 gataaagctc gttcaacctg atgtgctggt aaaaggtgcc gactatgaaa ttgaaaaaat
     6601 tgttggctac gatatcgtta tacaacgtgg aggcaaggtc attaccatag atttaacaca
     6661 gggatattcc actacagccc tgctaaatcg catcaaacaa taaaaatttt taaataaagt
     6721 ttctgaattt aaaagctttg ttgtaaattt acctttcaat tatagaactt atgaagcagg
     6781 ctgctataaa aattccaatt gttgccttat cactactact attaatgggt tgtgaaagca
     6841 atcacttctt taaatcagaa aaaaaattaa agagtgatat tgttggtaca tggaacagag
     6901 tattgtttgg gcaacagaat gaagaaacat ggatttttgt tgatggtgca ataaaaattt
     6961 atcagcctaa ccgtcatggc gatactgctg atgtgggtac attttctatt gatgctacat
     7021 tagcatcgcc ctatttaaag attgaaaata caagctattt aaatcagaat ccagggggcg
     7081 acacttataa tcacaagtgg tcaatagtaa aacttgataa taaagttctg gaaattgcta
     7141 cagacagccc gctgggagga gtagcacaga ttgaatttat caaacaatga tatcttagcg
     7201 tcatcatttt ttttcagacg ctgtaatggc taaagtaaaa tcagtttttg tttgtcagaa
     7261 ttgtggcgca cagtcaccca agtggatggg caaatgtgca tcatgcggtg agtggaattc
     7321 ttatgttgaa gaacttatca cctccggaaa ttcttcggca caggtaaatt ataaacgcag
     7381 cagcaagcct gaaaaaatta cagaaataga gttgcatcag gagcagcgaa ttgtacttcc
     7441 cgaccatgaa ctgaatcgtg ttctcggtgg cggattggta cgtggctcac tcattctctt
     7501 aggtggcgaa cccggtatag gaaaatctac tcttttactt cagctcgctg taagattaaa
     7561 aaatctgaaa acagtttatg tttccggtga agaaagcggt cagcaaattc gtatgcgtgc
     7621 agaacgactt aattcagacc tgcacagcca atgttatcta cttacagaaa catcacttga
     7681 aataatttta caacacctca atgcattgaa acccgacatg gttattattg attctatcca
     7741 aaccatgttt cattcgcaga ttgaatcatc accgggaagc gtatcgcaaa tacgaggatg
     7801 tgcaacagag ttgctgcgtt ttgcaaaaga cagtggttgt cctgtatttt tagtcgggca
     7861 tattactaaa gaaggtgtca ttgcaggacc aaaagtttta gaacatattg ttgatactgt
     7921 gcttcagttt gaaggtgaca ggcatcatat ttaccgtttg ttgcgcagtc ataaaaatcg
     7981 ttttggctct acagcagaaa ttggcattta cgaaatgcag ggtaatggtc tggcagaagt
     8041 atcggaccca tcgcagataa tgctttcgca acgtgatgaa gcactcagtg gcattgccat
     8101 tgcctgtaat cttgaaggca tgcgcactat gttgattgaa acgcaggcat tggtaagcag
     8161 tgctgtttat ggcgtgccac aacgttcaac aacagggttt gatcaaaggc gacttaatat
     8221 gttgcttgca gtacttgaaa aaagatgtgg gtttaagttg ggtgctaaag atgtttttct
     8281 caatattacc ggagggttaa aggttgatga tcctgcaatt gatttagctg taatttgtgc
     8341 tatactttca agtaataatg atattcctct tgagccgctg acatgcttta cgggtgaagt
     8401 tggattatca ggcgaaacac gtccggtaag ccgtattgaa caaagaataa aagaagccga
     8461 aaaacttggt ttcaaaaaaa tctatttccc tgcacagaat ctggctgcct tcgataaagc
     8521 catgtttaag aacattaaac tttatccggt tgaaagggta gatgaagttt tttcattgct
     8581 ttttggataa ttttttctga aaagacttct ttattcaaaa aatccattat attttagtcg
     8641 gtaaatattt ctttatttaa atagaaaaac aatgaaaacc ttaatttcag cagtaatagc
     8701 tatggtttca tttaccatta gcgcatcagc acaaagcaac attgaaattg gcatgtcggg
     8761 tggtgccaca aattattttg gtgatttggg taataatgaa gtactgcaat ctacttcaac
     8821 acgacccggt ggcgcaatta ctatccgcaa ctttttaaat agccctgcta agacaggtta
     8881 tcagtatcag ccattatctg ttgaagcacg ttttagctgg catcgcatcg gctatgatga
     8941 aacaaaatct attggcgata aaaaaggata ccaactgcgt aattacggaa ggggtttaag
     9001 tttcaggaca gatgtttatg gtgtgtcaac acatgttagc tatactttat atgccaataa
     9061 aagaaagcca ttgtatgcac aaggagcagc aatgtacttt tatgcaggtg tgggcattta
     9121 ttatgcacgg cctaaagcag atttatttaa tggaagcatt gacatcaaca accgttatta
     9181 tttctggagc gatggcacca tcagagatca ggatgaaaaa actggacaag gcaatataat
     9241 aaagcgtgat ggtaagtacg aaaccaacct tgatgaatgg cgcacagaag gacagggata
     9301 taacagtgag ttggggcaga aaaaaccata caaccttatg cacataggtt ttccattggg
     9361 aatgggatgg cgctggggta tttctaaaaa cattattctc tcggcagagc tgagctatat
     9421 aaaatttatt acagattatt tagatgatgt aagcgaccgc tatgcaacct acgaagaaat
     9481 aaaagcaaat ttccccgaag aacataagca ggacttggcc aagtatatct ctgacccttc
     9541 cggtaagggc acaaatggtt ttgaaggtgt tgcaacatca caacgtggca accatagaaa
     9601 aacagatggc tatgcatttc tttcacttga agttgcctac agacttgatt ttaactgggg
     9661 aaaaataaaa tcatcttttt aagaaaattg ctttttactt tttgtatcgt ttaatcattg
     9721 ttgcattttt gcagcaatgc aattaacaac cattcctttt caacttcgtt ttaggaagcc
     9781 atttgtttta gcttcgggaa caagaacaca tacatcagta gtttatatta aagctataca
     9841 aaatgaaatt accggctggg gcgaagctgc aatgcctccc tatgtaaaag aaacacaaga
     9901 aagtgtaata cattttgtac agcaactgca tattcctata ataaacacgt tagatgattt
     9961 aaacctattt tttgaaaaac tatatcaaac atcaggaaat tattttgcca aagcagccat
    10021 caatggagcc atgctcgatt ggtggacaag aaaaaatagt ctatcactgt cggaatattt
    10081 tggtattgat aagaaccttt caacacctga ttgttgttat accattacaa atggtgatga
    10141 tataaaacat tcaattaagc aagcagtaga ttttaaatta ctgaaaataa aagcaggctt
    10201 tgatggtgat gttgagttta taaaagaaat tgctgcaatt agtgaaaagc agatttgtat
    10261 agatgctaat caaggttggg ttaatgtgaa tgttgcatta caaaaaacag cgcagctgaa
    10321 aaatttgaat attgctttta ttgaacaacc actacctgtt gacgattggc aaggtatgca
    10381 gaaactaaaa caacatacca catttaattt tatagcagat gagtcatttc agactattga
    10441 tgatttaaaa aaaataatgg attgtttcca tggagtaaat atcaaactga tgaaatgcgg
    10501 tggcgtatca aacgcttttg aaattgcaaa atcagcacga cagttaaatg ccaaactgtt
    10561 attgggttgt atgagtgaaa gcagttgtgg tatagcaaca gctggagcca tacaacaatt
    10621 atttgactgg gttgatcttg acggaccctt attaatcagc aataatcctt ttaccggagt
    10681 cagatatcaa gaagggaaaa tatttattga aaataatgta ggcacagggg ctttgtctgt
    10741 tgaatctact ttgcaggctc tttaaataca agctccatca ggtgtacgtc aacatagttt
    10801 ccgaatttaa agcctacttc tttcattaca ccaatgtgtc gaaaaccaaa aatggtatgc
    10861 aaatgaatgc ttgcctcatt acctgatgca attcttgaaa gtagatagtg caaccctttg
    10921 ctttcacctt taagaattaa tatctctaaa agagctttac ctattccttt gccacggtga
    10981 tctttatgaa cataaacaga aacttcagca gtaccattgt aagcacaacg gtcgctccag
    11041 gcggttaggg atgcccatcc gcaaagcaca ccattatctt cagcaacaat tatgggatgg
    11101 ctgctgtcat gatttttaaa ccaaagcatc ctgtcttcaa tactttttac ttctgtatcg
    11161 aaggtagcag ttgtattcaa aatagcatca ttataaactt cggtaataaa cggaatatcg
    11221 tctgcagtgg ctgatctgat ttctatcata agttgtggta tagaaataca aaagtataaa
    11281 caatacgata tttttctgtt gaaagtaatt aatggattac aaaaactaag atagagctaa
    11341 aattcttaca agcggttttt taaatgaata cagtgtttgt ttttgttttt tcagagatat
    11401 aacgcaaatt aatatttgaa aatttcaacg attcttttaa aaaaacttac agtagtaaga
    11461 gttggaattt ggtgacaaag aaaacacaca atgtggtaat ggatttgcaa ttacccctac
    11521 aaattcaaaa caaaaaaaat atggacttat tacttgcaat cttaatttca atgggaatca
    11581 atgtaacccc aaaagattta actaacaaag agtttatgct caaaaatcaa agtgcaatac
    11641 aacatgcaca ggcaatttta aaagagcgtc agacagagtc aacacgcata acttttaagg
    11701 ctgcgccaca gacaaatatt ttcaagcaat ctaaaaatta atttccgtaa catctttcac
    11761 tgatataaag ccgcctcagt ttctgaggcg gcttttttat ttatagtaat actttatcaa
    11821 tatccggaaa acggaaatgt aacaagaaag tatcgtttaa tattttaaat tactgaatca
    11881 aatactttct tacaataaaa atagcagtat tttcaattac ttttaagtta tttacctctg
    11941 aaagagtatg tagtcagaaa tgatgaaagc tgaaatctta caaaagaaag ttacttactt
    12001 aaaggagatt tttaaaaatg aattcggcaa tgcatttgca attactccta caaattcaaa
    12061 acaaaaaaaa tatggactta ttacttgcaa tcttaatttc aatgggaatc aatgtaaccc
    12121 caaaagattt aactaacaaa gagtttatgc tcaaaaatca aagtgcaata caacatgcac
    12181 aggcaatttt aaaagagcgt cagacagagt caacacgcat aacttttaag gctgcgccac
    12241 aaacaaatat tttcaaacaa tctaaaaatt aatttccgaa acatctttca ctgatatata
    12301 gccgcctcag tttctgaggc ggcttttttg tttaaaatat tagtgtgcat gttgaaaatt
    12361 atccaaacaa tttataaccg aaatccacga tgcaaatttg ataagacccg aaatggattt
    12421 aaattttatt tcaacagcac ttcagaatcc ttctctacat caaaagtata gcatatttct
    12481 ggaaagagta caattgagct atctgatttt atatccaatc agaatcccca ctgatttaga
    12541 atatggaaga gccttttatt taagttaaca caactgctac gtttttctac cccattttaa
    12601 tataaaacaa caataaattt taaagttccc ttcattatta ttttttcatc tgttaagtaa
    12661 catctcttag tatttcttac ataagatttt tcaggtttaa cagcaactta aagaaaattt
    12721 ctcctctgaa aacaggaaat aaactcttac agttagttca tcattcttac aaaagaaaga
    12781 aagcccattt cgaggtcaga aaaaactgag tagcggcaat tggtttgcaa ttacccttac
    12841 aaattcaaat aaaaaacaat atggacttat taatctcaat tttactgtac ttaggtgttt
    12901 atgctacacc ggctgatctt caaaacaaag agtttgttgc taaaaattca gaacaaatca
    12961 acaaagcaga aatgctttca aaagatccaa actttaaatg tgatggaatt gtaattgtcg
    13021 gcagcggtaa tttgtaatta acgtctccaa aagaatatca aaggcgctga ctttaaatgg
    13081 tcagtgcctt tttaattttt ctgaaatggc tgcccagagt ttttcataag gaattacttc
    13141 aagcccttca gtattaaaaa atttattgcc ggcaacaggt tttaattttt ctaacgatac
    13201 agcagtttgt ttctgtgtct tagttttttg aaagttattt ttaaataagg tgagcaacat
    13261 gttcagaaaa atatatgtac gtggataaat tctcttatct acatattttt taatatacga
    13321 ttccagataa cttgttctgt cataaagttt gtcatagtcg cccatttcaa tcatcactat
    13381 tatctgacca atcatgatga taatgttatt gtcttcttta tgtgtggtgt agtagcttat
    13441 ttcatccaaa aagcccaaca agttgatgct ttcttttgga aaatgaccgg gtaagataaa
    13501 attaaggtaa ggttcaaaat aatgccaacg ctcttttact tcctgattgg agttgataaa
    13561 gtattcactg cgtgttgctt cgtaataaat gtttaatgcc tgttcaaaat tgcctgagtg
    13621 catacaaaga ataaagtaat gctcgagcgc atgcagccag tcagacgagt tggcactgag
    13681 tttacctaaa aaaatccgtg cttcttcttt acctttttcg tactgcctga ggtggatgta
    13741 ggccaacatt cttgaaagcg caataaaact tttctgaaag tcggaatagt attcgggatg
    13801 attctgataa aacgcttcgc gttcattcaa ggcttcttct accaattcaa aattactgct
    13861 gaaatagcca taaataaaag caatgttgcc gtaataaaaa tttgaattga atgttttaag
    13921 ttgctgatgt atttttttag ctctcagata atatcgttta gccttggcag catttttata
    13981 ctgtgaaata tttgaagcta cctgaaaaat atccagttct ctctgcatca atatcaactc
    14041 ggcttctaat tcaaactgtt tgatagattt tttaaagcgg tttctgtaat gataaaaatc
    14101 gtcaggttta tttttaatag ccatcctgtc ggcaagcatc aggtcggtaa ggcaaccaat
    14161 catgccatca tttgtaaatg ttgcatagtc tgctgctttt ttaaagaaat ggtcagaaaa
    14221 aaaagcggag gcattctgct gacgtaatat catggcagca ccaaaataaa tgtatgcacg
    14281 aaaagaactt cgccccaaca tggagttcat cagtttttcg ggatctaacc caaaaaccag
    14341 atttaccaaa cgatcttcaa gttcagcttt cagttgctga taggcaggat ttgtggctgt
    14401 gctttgtaat agtttttcgg cagcttcctg atcgttgttg atgctgcctt tcgaaatctc
    14461 tttataaaac caaagcagct caggataatt tttatcagca aaataaacat caggcacacc
    14521 aagaaaatca tgacggtgtt tagcaaaaaa attaattaat aatttgattt cagaaaactc
    14581 catcttatac ccgataaatc aatgctttat tttctttcaa atataggtca tcaataaatg
    14641 atgtactgca acgttgaaat tttatttttc agatggcttg ttaacagcct gcattatatt
    14701 tcaagcttgg tgcaggctct ttctgctgca atttgctctg cttttttctt tacaaaatcc
    14761 atccctgtgg aaacttcttc tccgttaatc atcaaccgca ctcttatgag tttgtcattg
    14821 cttttttcgt cttcaacagt ttcaaaggca acagtgtttt ttgttcgctg cgaccagttg
    14881 agtatgcggc ttttaaaatt tatttcagtt gcttccagcg attccagatc aacatggtgg
    14941 ttaatcattc tttcgagaat aaactgacgg gctttgccgt agcctctgtc tatataaata
    15001 gcaccaatca atgcttctaa tgcatcgcca taaatgttgc gaaacgaatc ggggccgtgt
    15061 gtttttttta taaaatgatt tataccaagt ttgaaggcaa gatgttttaa attttcgcgg
    15121 cttacaatct tgcttcgcat ttcagttaaa aaaccttctt cgcggaacgg aaatttttta
    15181 aaaagcattt cggctacaac ggcaccaagc acagcatcac caagatattc taatcgctcg
    15241 tttgaatgtt ttatgccatt ggtaacttct ttagtttgcg aacgatggct aaaggctaac
    15301 ttgtataacg ataactttat tggataaaag ccaagaatat tatgcagcga aaagtatgtt
    15361 ttgcgatcgg ctgaaaataa aatgttgata ggaaagaagc cgtaaaatct cactgattat
    15421 cagggagtga attttttgaa aattacagag gcattgtgtc cgccaaagcc gaatgtattg
    15481 ctcagcgctg ctctgatgtt gcgttgttgg ggtttatgga aagtaaaatt caaccgtgga
    15541 tcaaaagtag gatcgtcagt aaaatggttg atagttggtg gtacaatatt attttgtact
    15601 gctaaaatag atgcaattgc ttcaatagct ccggcagcac ctaacaggtg tcctgtcata
    15661 gatttggttg agctgatgtt cagcttaaat gcatcgtcac caaaaagttt tagtatggct
    15721 ttaatctcac ttgggtctcc cacaggtgtg cttgtaccat gaacattaat gtagtcaatg
    15781 tcggcagagg tcattcctgc atcgtcaagc gaagatttca ttacgttaaa agcaccaaga
    15841 ccttcaggat gtgttgctgt aagatgataa gcatcggcac tcatgccacc gccaaccatt
    15901 tcagcataaa tttttgctcc gcgagcctgt gcatgactaa gctcttcaag aacaatacaa
    15961 cctgcacctt caccaagcac aaatccatca cgatctaaat caaaaggacg tgaggcagtt
    16021 tgtggtgagt cgtttctctc gctaagtgca tgcatggcat tgaaacctcc aatgcctgct
    16081 tcgttaacag aagcttcaga gccacctgaa ataataatat cggctttgcc taaacgcaga
    16141 taagtaaatg catctatgag tgcattggtt gatgatgcac atgcagaaac cgttgtgtag
    16201 ttagggccac gatagccaaa acgcatagag atatgtcccg atgcaatgtc ggcaatcatt
    16261 ttaggaatga aaaaagggtt gaaacgtggt gttccgtcac ctttagcata ggcaataacc
    16321 tcgtcaagaa aagtacgaag tccgcctata cccgatcccc agataacacc tactctgtca
    16381 tgattaatgt tggcagtatc taagcccgca tcttttactc cctgatcgga aacagcaata
    16441 gcatattgcg aaaacgggtc aagcttgcgt gcttcttttt tctctaaata aacaagcggg
    16501 tcaaaatttt taacctcgca ggcaaagtgt gttttaaact ttgaagcgtc aaactttgta
    16561 atctgacaag caccgcttac accgttgagt aaaccgtccc agtattctga aacagagtta
    16621 cctaatggtg taagggcacc taatcccgtt actacaactc tcttgagctc catgtaaggg
    16681 acgcttattt tacgttagca gtaatataat ctacagcttg tcctacggtt gagattttct
    16741 cggcctgatc atccggaata gcaatgttaa actctttttc aaattccata atcagttcaa
    16801 ctgtgtcgag cgaatcagct cctaaatcgt ttgtaaatga cgcttcatta gttacttcac
    16861 cttcgtcaac acctagtttg tcaacaataa tggcttttac tctggatgca
//
LOCUS       DAOOOL010000045        16797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_281_length_16797_cov_47.738383, whole genome shotgun sequence.
ACCESSION   DAOOOL010000045 DAOOOL010000000
VERSION     DAOOOL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16797
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_16797_cov_47.738383"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="rsgA"
                     /locus_tag="PKU82_05425"
     CDS             <1..786
                     /gene="rsgA"
                     /locus_tag="PKU82_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOZ82349.1"
                     /translation="VGDYVKYYFEGDDALITDLLDRKNYIIRKSKKLSKQVHILAANI
                     DHAFLVATPVLPRTSTGFIDRFLATAEAYSIKASIIFTKADIYNRDILNYVDELKNLY
                     SGIGYSVYVVSSQIPDSLIPLRTQLKDKTNLFTGHSGVGKSSLINVLIPGLNLKVTPI
                     SEQHLTGKHSTTFAEMHQLPEGGFIIDTPGIREFGTYDFNKNDVTHYFPEFFQLLPQC
                     KFNNCIHVNESGCEVLAALERGEVSPSRYHNYLSILNNEDIFR"
     gene            790..1248
                     /gene="dtd"
                     /locus_tag="PKU82_05430"
     CDS             790..1248
                     /gene="dtd"
                     /locus_tag="PKU82_05430"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOZ82350.1"
                     /translation="MKVVIQRVITASVEIEGAIYSSIHNGLLVLLGISVDDEKEDVDW
                     LCKKIVGLRIFSDENGLMNLSVKEINGQILLVSQFTLYGDANKGNRPSFIHAARPEKA
                     IPLYEYFIQQLNMNDIVVCTGKFGADMKVSLTNDGPVTIIIDSKKQTNDH"
     gene            1238..1564
                     /locus_tag="PKU82_05435"
     CDS             1238..1564
                     /locus_tag="PKU82_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOZ82351.1"
                     /translation="MTIKDSQQIVDDWIKKHGVRYFNELTNLALLTEEVGELARIIAR
                     KYGEQSAKNNEDSLEMADEMADILFVLICLANQTGVDLEQAMKKNLEKKTHRDAGRHL
                     KNKKLQ"
     gene            complement(1565..2302)
                     /locus_tag="PKU82_05440"
     CDS             complement(1565..2302)
                     /locus_tag="PKU82_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82352.1"
                     /translation="MKTSKLNLNFFIVIILIAFFSTGCSPIYYVPSQVNMPSLTKGGD
                     AKFSASFALPQFNGVAQSFDGQLSYSPVKHLGVTVDGLAVVGTGKDKDYSGSGKSFGF
                     ALGYYNMIDENLGVEFYAGYGGGDVKLNRDSLKFFSADVRRFYIQPGIYRSTKRFNFG
                     IAFRFSNVQYSNLVFYQIPEIGDKIVEDNYFFYEPSFYCSLGGNLVKVKFQLTPSFNI
                     DNKLDFNRQKLAIGLGVDFLINRKNRD"
     gene            2509..3381
                     /locus_tag="PKU82_05445"
     CDS             2509..3381
                     /locus_tag="PKU82_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOZ82353.1"
                     /translation="MSHQKAIQTTWISIAGNILLAVVKWITGYFGNSYALIADAIEST
                     TDIFSSLLVLLGLKYASRPADKNHPYGHGRVEPLVTFLVVGILVASATLIAVESIRNI
                     MTPHQTPESYTLIVLAVIIIFKELFFRFVKKRNSQINSTALKADAWHHRSDAITSLAA
                     FIGIAIAIFMGKGFEAADDWAALVAAGFIFYNSYLIFRPALGEIMDEHLYEDVVEDIR
                     KVAGKVEGVIDTEKCYVRKTGINFLVDLHITVSSHISVKAGHQIAHHLKDVLIKHNPL
                     IADVLIHVEPDEHV"
     gene            complement(3407..4273)
                     /locus_tag="PKU82_05450"
     CDS             complement(3407..4273)
                     /locus_tag="PKU82_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOZ82354.1"
                     /translation="MRQLKITKSITNRESASFEKYLQEIGKEELITAQMEVELARKIR
                     AGDQRALEKLVRANLRFVVSVAKQYQSQGLSLPDLINEGNLGLIKAAKRFDETRGFKF
                     ISYAVWWIRQSIMQALAEQARIVRLPLNQIGAANKVKKAFSSLEQKFEREPTAEELAN
                     ELEIDTDRIAYTMKMPGRHVSMDAPFVPGEDNTLLDVLTNPDAPYADSTLMAESLHTE
                     INRSLETLPEREKDVIKLFYGIGVEHGLSLEEIGEKFDLTRERVRQLKEKAIKTLRQN
                     SKSKLLKAYLGQ"
     gene            4767..5225
                     /locus_tag="PKU82_05455"
     CDS             4767..5225
                     /locus_tag="PKU82_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82355.1"
                     /translation="MIKTTNIFTALVCLLLVTSLSVLGQKGKVNQEKKITGPGKCLIK
                     SDSLLTNELTKEDLIKWVENPPFIVNCEDSKTYKLKFYEFTILKLKPFENKSFGVGDD
                     RMIPILGRKAIDDLKEGDTVILKGVDLIEDGTDKTTKAPTISIKIIEKKP"
     gene            complement(5222..6139)
                     /locus_tag="PKU82_05460"
     CDS             complement(5222..6139)
                     /locus_tag="PKU82_05460"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOZ82356.1"
                     /translation="MIFLIGIFSNSFCQDSVNTQRKIPFFEPSPVYNKQRVQIAGYTT
                     LGLYAGTMAGLYQLWYSGYDMNHFHFFNDNGEWLLMDKTGHTFSSYIAGKQGYNVCCW
                     AGMDNKKATLIGGNLGWIFLVSVEALDGFSSAWGFSWGDIAANTLGSSVFIGQQLLWQ
                     EQRIEFKFSFHQSKYAHYRPDLLGENYTQQLFKDYNGQTLWLSANINSFIKKTNLVPA
                     WLNIAAGIGAEGMTGAFDNATEYKNNPIPNFKRYRQIYIAPDIDLTKIKTKSKALKLF
                     FEVTRFIKFPLPALEINSKGKWLFHPLYF"
     gene            complement(6295..6666)
                     /locus_tag="PKU82_05465"
     CDS             complement(6295..6666)
                     /locus_tag="PKU82_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOZ82357.1"
                     /translation="MQHDDLHIEKLYYSIGEVADMFKVNTSLLRYWEKEFTMLHPKKN
                     LKGERHYTKKDIESFRIIYHLVKEKGFTLSGAKQYLKTNGKAIPVAEVSENTEVKEDN
                     SHVKFALNRLKAFLIEMKGNL"
     gene            complement(6697..7668)
                     /locus_tag="PKU82_05470"
     CDS             complement(6697..7668)
                     /locus_tag="PKU82_05470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOZ82358.1"
                     /translation="MAKAKYYFNTHSLKYEKVVVSFKKRFLRVLGWLATAMVFSAIII
                     ALAYQFLDSPKEKQLKREIAVMDLQYEILQQRMDLMSKVLKDIEYNDDNIYRVIFEAD
                     PIPESVREAGYGGVDKYRSLKGYDNSSLMIETTTQLDKLMRQMYVQSKSFDEVFELAK
                     NKKELLASIPGIQPVSNKELTRIASGFGYRIHPIYKTSILHTGIDFSAPIGTEIYSTG
                     NGVVKKVEKEGRGYGNNVTINHGFGYETLYAHMSKIIVKPGQKVIRGQIIGYVGNTGS
                     STGPHCHYEVIKNGSKINPINFFFNDLTAAQYEQVLEISSKYNQSFD"
     gene            7802..10420
                     /gene="alaS"
                     /locus_tag="PKU82_05475"
     CDS             7802..10420
                     /gene="alaS"
                     /locus_tag="PKU82_05475"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOZ82359.1"
                     /translation="MTSSEIRKTFLDFFQSKQHTIVPSAPIVVKGDPTLMFTNAGMNQ
                     FKDWFLGNQPAKYRRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDPND
                     PNAGYFKKEAIAWAWELLTEVYKLPKDRLYVTVFEGDKKENLHFDEESNTCWKQHIDH
                     SRILMGNKKDNFWEMGDTGPCGPCSEIHVDLRSKEERKQTDGKILVNTGHPLVIEIWN
                     NVFMEFNRKADGTLEKLPARHVDTGMGFERLCMAIQQKKSNYDTDIFQPTINYIEAMS
                     GIKYGSSEQTDIAMRVLSDHIRAIAFTIADGQLPSNNKAGYVIRRILRRAVRYSFTYL
                     NFKTPFIHRLTPLLADQFKEVFPELFEQKDFVQKVIKEEEQSFLRTLETGIRRFDLYN
                     KSVIDGEFAFELYDTYGFPIDLTQLIARERNMQVDMNGFNQCLEQQKERSRAASAVDA
                     EDWVILENSESKFIGYDNTTAEVNIIKYRKVKSKNKEQYQLVFNQTPFYAESGGQVGD
                     TGYIQNEKEKIFITDTQKENNQIIHLSDKLPVDLKADFIATVNNSRRRKITANHSATH
                     LMHAALKQILGKHVVQKGSLVNDEHTRFDFAHFAKVSDEDIAKIEQLVNEKIRENIAL
                     DERRNVPIEEAKSMGATALFGEKYGDYVRVIIFDPAYSMELCGGTHVNATGEIGLFKI
                     LSESAVAAGVRRIEAITSIEAEKYIINQLTQLNTIKTELKNPKDIVIRVKELLSENEI
                     LQRQIDGFVHEKAQLIKADLLKNVKLQNGINVIAQKIDLTNADAIKNISFELRNQIPD
                     SFILLASENDGKAMLSLIISDSLVQSGNLNATTVIRNISKHIQGGGGGQPFYATAGGK
                     NPAGIDAAIEEALKMI"
     gene            complement(10475..11326)
                     /gene="gldN"
                     /locus_tag="PKU82_05480"
     CDS             complement(10475..11326)
                     /gene="gldN"
                     /locus_tag="PKU82_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOZ82360.1"
                     /translation="MKKVLLFCFLFVMTFMVKQTNAQTVLDGVYVKEHNPTRKVMEYS
                     FLREADVLWAKRVWEVIDLKQKINLPLRFPREGDTKDRKSLIEVLMNAIDEGSLTAYS
                     TTDDEFTTPMTLEEFKKIGGAGMDTIQITDPEPPYLTRDSAIQRTFSRDKVIAYRIKE
                     EWFFDKQRSVMECRIIGIAPLMLAEDEYGNIREGNIQKPLFWIYYPEARKLLANAEAF
                     NRENDTERRTFDDVFHKRMFNSYIIKESNVYNRRIEDYKTGLDALLEAERIKSEIVNL
                     EHDMWEY"
     gene            complement(11357..12961)
                     /gene="gldM"
                     /locus_tag="PKU82_05485"
     CDS             complement(11357..12961)
                     /gene="gldM"
                     /locus_tag="PKU82_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOZ82361.1"
                     /translation="MASGKLTPRQKMINMMYLVLTALLALNVSKEIINAFITIDEGIT
                     ASNNNVIKKNNQVMDAFATAAQADAVKAKPFQEKATQVHKASDDLYKYLEDLKNEIVR
                     KADKLEANAKIPAPREIQKNDDYDTPTLIMCGDKQDGKGFKATELKKKLEDYKVKLIG
                     FVADKDKKDFQSRFDELINLKDPDPASKLAKEEKKKTWEMATFYHTPVVAALAMLSKI
                     QSDVKTAESQVNNYLLAQINAKDLKFTDVEPKVVAPTSYVLTGQDYKADVFLAAFNKS
                     SDAEIVVGGRSLPVENGLGKYVDRPSSEGVKKWGGVIKVKDPEDPTKVKEYPFEAEYI
                     AAKPAAVVSPTKMNVFYIGPENPVSISVPGVPNNKVVASISGGGGSLTKNPKGTGADY
                     IVKVTTQGEATISIIADMDGKKINMGAQKFRVKRVPDPVAKIAGKREGPMQKNALIAQ
                     SAVIAELENFDFEIYYKVTKFKMSLYPKGKDPIELESNNNLITANMKAALSKARAGDK
                     VYFEYIKAVGPDGGTRSIPSVNFTIQ"
     gene            complement(13018..13827)
                     /gene="gldL"
                     /locus_tag="PKU82_05490"
     CDS             complement(13018..13827)
                     /gene="gldL"
                     /locus_tag="PKU82_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOZ82362.1"
                     /translation="MGLAEFTSGKKYKNFMAKLYGIGAAIVIAGALFKIQHWEGAGIM
                     LTVGLLTEAIIFFFSAFEPLHEETDWSLVYPELAGMHDPSQGRDKKKKIDPVSQALDK
                     MLSDAKIGPDLIASLGTGLKSLGDNTAKMADLSDASVATNDYVKNVNAATKSVTELSG
                     SYQKAAQAMSSLSTASDDVKMYNEQVQMAGKNLGALNAVYELQLQDSNNHLKQTSKFY
                     DGINQLMSNLNESLDDTKKYKDEVSKLAKNLSSLNTVYGNMLSAMNLNPNK"
     gene            complement(13902..15164)
                     /locus_tag="PKU82_05495"
     CDS             complement(13902..15164)
                     /locus_tag="PKU82_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ82363.1"
                     /translation="MRKLFIPIIAVILLSSCGGGNKGQLVGALNRERWYQADPYGMVF
                     IPSGAYNMGPSDQDVPYAMVAQSKSVSVHAFYMDNTEITNNEYRQFVEWVRDSLAHRL
                     IGGDHLIEEGEYGERINWRKKIKWEDEQNVETLAELFLPESERFYRRKEIDSRKLNFE
                     YYWIDLKEAAQKSNREQGMTDRSVFIKKDIINVYPDTLAWIHDYTYSYNDPVTNMYFW
                     HPAYDDYPVVGVTWKQARAFCIWRTQLLNSYLQENGESFVQDFRLPTESEWEYAARGG
                     HDLAPYPWGGPYIRNSRGCFLGNFKPMRGNYMDDGGFYTVKTTSYWPNDYGLYCMSGN
                     VSEWTSNAYEEAAYNFSHDLNPDYSYDAKDSDPVALKRKVIRGGSWKDVGYFLQTGTR
                     SYEYQDTAKCYVGFRCVMSFLGRDKADF"
     gene            complement(15314..16216)
                     /locus_tag="PKU82_05500"
     CDS             complement(15314..16216)
                     /locus_tag="PKU82_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ82364.1"
                     /translation="MKRLFTVVAVATASVSFGQQDPQFSQNMFNRLYPNPASAGTNEA
                     ICGTLIYRDQWDKFGGGPKTAVFGVESPVEVLHGGLGLSVLTDKPLNQKNLNIKLAYS
                     YHLDLGSGKLGIGLDAGLLQRGYGNDFNAIDPDDPLVKSGTGSKFDLGAGLYYKSDKF
                     YVGASATHLTGGKIEYTDDAKVEVVPHFYFMGGVDFDLTPSLKLKPSLFIKSDAKETQ
                     ADINANLHIKDKVWIGASYRLQDAIVLMAGINIIENLRLGYSYDITTSKLKGYHSGTH
                     EIMLGYCYVIKPKVIPVLRNVRFL"
     gene            complement(16345..>16797)
                     /locus_tag="PKU82_05505"
     CDS             complement(16345..>16797)
                     /locus_tag="PKU82_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOZ82365.1"
                     /translation="DASATSTPSPNGLTGDMACQIMYYAGMSDKLEGIGLYEYAASID
                     TNQMTAALISQMVWYFMEGVSNRKNDFPDSTSSQFTVYNLTLRENNEEMHFLKSNVTG
                     RWWMEIPLSKIKKKLDRHQLVPCSYDDYQKACNNEIPERWWQALKKLS"
BASE COUNT         5335 a         3110 c         2845 g         5507 t
ORIGIN      
        1 gttggtgatt atgtaaaata ttattttgaa ggcgatgatg cattgattac agatcttctc
       61 gacagaaaga attatattat caggaaatca aaaaaattga gtaagcaggt tcatatttta
      121 gctgccaata ttgaccatgc ttttttggta gcaacacctg tgctgccaag aacatcaaca
      181 gggtttattg acagatttct tgctactgcc gaagcctatt ctattaaagc ttccatcatt
      241 ttcactaaag cagatattta caatagagac attttaaact atgtagatga attgaaaaat
      301 ctttattcag gaattggata ttctgtttat gtagtttcaa gtcagatacc cgattcatta
      361 attccattaa ggacacagtt aaaagataaa acaaatttat tcaccggaca ctcaggtgtt
      421 ggaaaatctt ctcttattaa tgtgttaatt cccggtttaa atttaaaggt aacaccgatt
      481 tctgaacaac atttaaccgg aaaacatagt actacatttg cagaaatgca tcaactccct
      541 gaaggtggat ttataattga cacacccggc atcagagaat tcggcactta cgattttaac
      601 aaaaacgatg tgacacatta tttccctgag ttttttcaac tattaccaca atgcaaattc
      661 aacaattgta ttcacgttaa cgaaagcggt tgtgaagttt tagcagcact tgaacgaggt
      721 gaagttagtc catcgcgcta tcataattac ctgagtattt taaataatga agatatattt
      781 cgttgagcaa tgaaagttgt catacaaaga gttattacag caagtgttga aattgaagga
      841 gcaatttatt cttccattca taatggtctg ttagtgcttt taggtatttc tgttgatgat
      901 gaaaaagaag atgttgactg gctgtgcaag aagattgttg gcttacgaat tttctctgac
      961 gaaaatggtt taatgaatct ttctgtcaaa gaaatcaacg ggcaaatttt actagtatct
     1021 caatttactc tttatggtga tgctaataaa ggtaaccgac cttcatttat tcatgcagcg
     1081 aggccggaaa aggcaatacc attatacgaa tattttattc agcaactaaa tatgaatgat
     1141 attgtagttt gtacgggaaa atttggtgca gacatgaaag tgtcattgac caatgatggg
     1201 cctgttacca taattattga ttcgaaaaaa caaacaaatg accattaaag attctcaaca
     1261 aattgttgac gattggataa aaaaacacgg tgtccgatat ttcaatgaat taactaacct
     1321 tgccttgctg actgaagaag ttggtgaatt agcaagaata attgcaagga aatacggtga
     1381 gcaatcagca aaaaataatg aagacagttt agagatggcc gatgaaatgg ctgatatttt
     1441 atttgtttta atttgtcttg ccaatcaaac cggtgttgac cttgaacagg caatgaaaaa
     1501 aaatttggaa aagaaaacac atagagatgc tgggcgtcat ttgaaaaata aaaaactaca
     1561 gtaatcagtc tctgtttttt ctgttgatta aaaaatcaac gccaagacca attgctaatt
     1621 tctgacgatt aaaatctaat ttattgtcaa tattaaatga aggtgttaat tgaaatttta
     1681 cttttactaa attaccacca agtgaacaat aaaagcttgg ctcataaaaa aaatagttgt
     1741 cttctacgat cttatctcct atttcgggaa tctgatagaa aacaaggttt gaatattgaa
     1801 cattactaaa acgaaatgca ataccaaaat taaatcgttt tgtactgcga taaattcccg
     1861 gttggatata aaagcgccta acgtcagcac tgaaaaattt aagtgaatca cgattcaact
     1921 ttacatcccc tcctccatag cctgcataga actcaactcc caaattctca tctatcatat
     1981 tgtaatatcc caatgcaaat ccaaacgact tgccgctacc gctatagtct ttgtcctttc
     2041 ctgttccgac aactgctaaa ccatctactg ttactcctaa atgtttcacc ggtgaatagc
     2101 ttaactgtcc atcgaaagat tgggcaacgc cattaaattg tggtaatgca aaagatgcac
     2161 tgaattttgc atcaccacct tttgttaatg atggcatgtt tacctgactt ggaacataat
     2221 aaattggtga gcaaccggtg ctaaaaaatg caattaaaat tattactatg aagaagttta
     2281 aattcagttt agaagttttc atttttccgg aataattata caggtcatta ttagaataaa
     2341 agttttgact aaagtaacta atattttata actatgtatt aattgctgcc atttcttgat
     2401 tgatgctttt gcctataaag tatccatctt tcaaaatgga taaatcttta attcagacga
     2461 agaaaatatt ttataaaact ataactttga agtttcaatt acaaaaacat gtcgcatcag
     2521 aaagcgattc aaacaacttg gattagcatt gcaggcaata ttttgctagc agtagtgaaa
     2581 tggattacag gatattttgg taattcgtat gcactgattg cagatgctat tgaatctaca
     2641 acagatattt tttcatcgtt attggtttta ttggggttga aatatgcaag tcgtcctgct
     2701 gataaaaacc atccttatgg tcatggacgt gttgaacctt tagtaacatt tcttgtggta
     2761 ggaattttag ttgcttcagc tacactaata gctgttgaaa gcatcagaaa tattatgaca
     2821 ccgcatcaga cgcctgaatc ttatacactt attgtccttg ctgtgattat tatttttaaa
     2881 gaattatttt ttcgcttcgt aaaaaagaga aatagtcaaa ttaatagtac tgccttaaaa
     2941 gcagatgcct ggcatcatcg aagcgatgct attacttcgc ttgcagcttt tattggaatt
     3001 gcaattgcca ttttcatggg taaaggattt gaggctgctg atgattgggc tgcattggtt
     3061 gctgccggtt ttatttttta caacagctac ttgattttca gacctgcctt aggcgaaatt
     3121 atggacgaac acctttacga agatgttgtt gaagacataa gaaaagtggc tggaaaagtt
     3181 gaaggtgtaa ttgatacaga aaaatgttat gtacgtaaaa caggtataaa ttttttagtt
     3241 gaccttcaca tcacggttag ctcacatatt agtgttaaag ccggacatca gattgcacat
     3301 cacttgaaag atgtgctgat taaacacaac cctcttatag cagacgtgct catccatgtt
     3361 gaaccggatg agcacgtttg aattttaaaa caggaatgtt ttctgcttac tgacctaaat
     3421 aagctttaag cagtttgctt tttgaatttt ggcgaagtgt cttaatagct ttttctttaa
     3481 gctgacgtac acgctcgcgt gttaaatcaa acttctctcc tatttcttct aatgataaac
     3541 catgctcgac accaataccg taaaaaagtt taataacatc tttttcacgc tcaggcagtg
     3601 tttctaatga acggttgatt tcagtgtgta acgactctgc catgagtgtg ctgtcagcat
     3661 aaggtgcatc aggattggtt aatacatcaa gcaatgtatt atcttctccg ggtacaaaag
     3721 gtgcatccat tgatacgtga cgtcccggca ttttcatagt gtatgcaatt ctgtctgtat
     3781 caatttctaa ttcattagct aattcttctg ctgttggttc gcgctcaaat ttttgttcaa
     3841 gactcgaaaa tgcttttttt accttgttgg cagcaccaat ctgattaagt ggtaacctaa
     3901 ctatacgtgc ttgctctgcc aatgcctgca tgattgactg acgaatccac catactgcat
     3961 aggaaataaa tttaaaacct cttgtttcat caaaacgttt tgccgcttta attaaaccaa
     4021 gatttccttc attaatcaag tcgggcaaac ttaaaccttg cgactgatac tgcttagcta
     4081 ccgaaaccac aaatctcagg ttagctctca ctaatttctc taatgctcgc tggtctcctg
     4141 ctctgatttt acgggcaagt tccacctcca tctgtgctgt aattaactcc tctttaccaa
     4201 tttcctgtaa atacttttca aacgatgcgc tttcacggtt tgtaatggat ttagtaattt
     4261 ttagctgtct catctatttt atctaatttt tatgtgtgtt tcaatgtatg caatgtaaga
     4321 acaaaaaacg ataatcatgg tttaaaattg cattattttt tttaaaggcg ttgcaaatat
     4381 actatgcgtt tagcggcaat gctaacttta tttttaagca atgccacaca atgctgctta
     4441 tcaacacatt atctttgcct tttcaagtaa aagactattt ttttaaccga ttttgagagt
     4501 tatttagcgg ttatttatga ttaaaaattc acctgaaaac agaaaaatta tttcaatggt
     4561 tattcatctt taatccaaat ttcatccttt gatgcaatta agagtaactg taagaaacag
     4621 cgtataaagt atatttttca gtagttatat ggccaccttt aaaccctaaa aagataaatt
     4681 agtacctgct aaaaaatatt tcttctacat tcgtgtttta ttcgaacaga aattaaaaca
     4741 aattggcatt taacaacaac cacatcatga taaaaacgac caatattttt actgcattag
     4801 tatgtttatt gttagttact tcattgtcag tattaggaca aaaaggtaag gtaaatcaag
     4861 agaaaaaaat taccggtcca ggaaaatgtt taataaaatc agatagtctt ttaacaaatg
     4921 aacttaccaa agaagaccta ataaaatggg tagaaaatcc accttttatt gtgaactgtg
     4981 aagatagcaa gacttacaaa ctgaagtttt acgaatttac aattttaaaa ctgaagcctt
     5041 ttgaaaataa gagctttggt gttggtgatg acagaatgat accgattctc ggacgcaagg
     5101 ctatagatga tctaaaagaa ggtgacacgg tgattctaaa aggagttgac ctgattgaag
     5161 atggaacaga taaaactaca aaagcaccta ctatcagcat taaaattatt gagaagaagc
     5221 cttaaaaata taagggatga aaaagccatt tcccttttga attaatttct aaagcaggta
     5281 atggaaattt tataaaacgg gttacttcaa aaaataattt caatgccttt gactttgttt
     5341 ttattttagt cagatcaata tcaggtgcta tataaatttg tctgtagcgc ttgaagttgg
     5401 gaatcgggtt atttttatat tctgtagcgt tgtcaaaagc tcctgtcata ccttcagcac
     5461 caatgcctgc agctatattg agccaggcag gtactaaatt tgttttcttt ataaaagaat
     5521 tgatgttagc agacaaccac aatgtttgtc cgttataatc tttaaaaagt tgctgagtat
     5581 aattttcacc taatagatca gggcgataat gagcatactt tgattgatga aaagaaaatt
     5641 tgaattctat tctttgctcc tgccaaagca attgctgtcc aataaaaaca gatgatccaa
     5701 gtgtgttagc tgcaatatct ccccatgaaa aaccccaggc agaagaaaaa ccatctaagg
     5761 cttctaccga tactaaaaaa atccaaccaa gattgccgcc tataagtgtg gcttttttat
     5821 tgtccatgcc tgcccagcaa caaacattgt aaccttgctt acccgcaatg tatgaactaa
     5881 acgtatgacc tgttttatcc atcagtaacc attctccatt atcattgaaa aaatgaaaat
     5941 ggttcatatc atatccacta taccataact gatataatcc ggccatggtg cctgcataca
     6001 agccaagtgt agtatatcct gcaatttgaa cgcgctgttt attgtaaaca ggcgaaggtt
     6061 caaagaatgg aatttttcgt tgtgtgttaa cagaatcctg acaaaatgaa tttgaaaaaa
     6121 taccaatcag aaatatcatg acaagccact tgtgtgtgtg gtgaaatggc aacaatctat
     6181 aaatggaaat taatttcttt ggtttcaact cacgcttcta taatcagcag caaaactaaa
     6241 agaaatgtaa atgaaattga aaattacaat caatgtgttt tctcgcatat aaattcaaag
     6301 atttcctttc atttcaatta aaaaagcttt taacctattc agagcaaatt ttacatggct
     6361 gttatcttct tttacctcag tattttcact tacttctgct acaggtattg ctttaccatt
     6421 tgttttcaga tattgtttgg ctccactcaa tgtaaaacct ttttccttta ctaaatgata
     6481 aattatccgg aatgattcta tatctttttt ggtataatgc cgttcacctt ttaaattttt
     6541 tttcgggtga agcattgtaa attctttctc ccagtagcgc aataacgagg tgtttacttt
     6601 aaacatatca gcaacttcgc caatgctgta atacagtttt tctatatgta ggtcgtcatg
     6661 ctgcatcatc agatgatttt tctataattc acaaatctaa tcgaatgatt gattgtattt
     6721 tgaagaaatt tccaacacct gttcatattg tgccgctgtc aggtcgttga agaagaagtt
     6781 tatcgggttt atcttgctgc catttttaat aacctcgtag tggcagtgag gccctgtgct
     6841 acttcctgta ttaccaacgt aacctataat ctgtcctctg attactttct gccccggctt
     6901 aacaataatt ttactcatat gtgcataaag cgtttcatat ccaaatccgt gattaatggt
     6961 tacattatta ccgtagcctc taccctcttt ttcaaccttt tttacgactc cattgcctgt
     7021 agaataaatt tctgttccaa ttggtgcact aaaatctatt cccgtgtgta aaatacttgt
     7081 tttatatatc ggatgtatcc tataaccaaa accagaggca atcctagtta gttctttatt
     7141 actgactggc tgaatacccg gtatagatgc caacaattct tttttatttt ttgcgagttc
     7201 aaaaacttca tcaaacgatt ttgactgtac atacatttga cgcatcaatt tatccaactg
     7261 cgtagttgtt tcaatcatca gcgatgagtt gtcataaccc tttaatgaac gatatttatc
     7321 aacgcctcca taacctgcct cccgcacgct ttcgggtata gggtctgctt caaaaatcac
     7381 tctgtaaata ttgtcatcat tatactcaat atctttaaga acttttgaca ttaaatccat
     7441 tctttgctgc aaaatctcgt attgaaggtc cattacagca atttcgcgtt taagctgctt
     7501 ttcttttggt gaatccagaa actgataggc taatgctata atgattgcac taaataccat
     7561 agctgttgcc aaccatccca aaacacgtaa aaagcgcttt ttgaaggaaa caactacctt
     7621 ttcgtacttt aaagaatgtg tattaaaata atatttggcc ttcgccataa tctttaattc
     7681 tatttttgca caattcagca aaaataaaat tttcctgaaa tctaattaaa gtcaaaaaat
     7741 tataaaaatc agttacttaa atatcttcat ttaaacttct tacaccattt taataaatta
     7801 catgacctct tcagaaattc gtaaaacttt tttagatttt tttcagtcga aacaacatac
     7861 catcgttcct tcagcaccaa tagttgttaa aggcgaccct acacttatgt ttaccaatgc
     7921 agggatgaat cagtttaaag attggttttt aggaaatcaa cctgcaaaat acagacgtgt
     7981 tgcagataca caaaagtgtt tgcgtgtttc cggaaagcat aatgaccttg aagaggtggg
     8041 tgttgatacc tatcatcata ccatgtttga aatgcttggt aactggagtt ttggtgaccc
     8101 taatgatcct aatgcaggct actttaaaaa agaagccatt gcatgggcat gggagcttct
     8161 tactgaggtt tataaacttc ctaaagaccg tttgtatgtt accgttttcg aaggtgataa
     8221 gaaagaaaat ctgcatttcg atgaagagtc aaatacttgt tggaaacaac acattgatca
     8281 ttcacggatt ttaatgggta ataagaaaga taatttctgg gaaatgggtg acacaggacc
     8341 atgtggcccc tgttccgaga tacatgttga cttgcgaagt aaagaagaaa gaaaacaaac
     8401 agatggaaaa attttagtca atactggcca tccattagtt attgagattt ggaataacgt
     8461 atttatggaa ttcaacagaa aagctgacgg cacacttgaa aaattgcctg ccaggcatgt
     8521 tgatacaggt atgggatttg aacgtttgtg tatggcaata caacaaaaaa aatcaaatta
     8581 tgacacagat atttttcaac ctaccataaa ttacattgaa gcaatgagtg gtataaaata
     8641 tggcagtagc gagcaaacag atattgccat gcgcgttttg tctgatcata tcagagctat
     8701 tgcatttacc attgcagatg gacaacttcc gtcaaacaat aaagcaggtt atgttattcg
     8761 aagaatactt agaagagccg ttcggtattc ttttacttat ctgaatttta aaactccatt
     8821 tattcataga ctgacacctt tattggcaga tcagtttaaa gaagtttttc ctgagctttt
     8881 tgaacagaaa gattttgtac aaaaagttat caaggaagaa gagcaatctt ttttacgcac
     8941 tcttgaaaca ggtatcagaa gatttgatct atataacaag tctgttattg atggagagtt
     9001 tgcattcgaa ttatacgaca cttatggttt tccaattgac cttactcaat taattgcacg
     9061 tgagcgaaac atgcaagttg acatgaatgg atttaaccaa tgtttagaac agcaaaaaga
     9121 aagatccaga gctgcatcag cagttgatgc tgaagactgg gttattcttg aaaacagtga
     9181 atcgaaattc atcggatatg acaatacaac tgctgaggta aatattataa aatacagaaa
     9241 ggtaaaatca aaaaataaag aacaatatca gcttgtcttc aatcagactc ctttttatgc
     9301 agagagcggt gggcaagttg gtgatacagg ctatatccaa aacgaaaaag agaaaatttt
     9361 tattaccgac acacaaaaag aaaataatca gatcattcac ctgtcagata aattgcctgt
     9421 tgatttgaaa gcagatttta ttgcaacagt caacaattcg agaagaagaa aaataacagc
     9481 caatcacagt gcaacacatt taatgcatgc agcattaaag cagattttag gtaagcacgt
     9541 agtacaaaaa ggttcacttg tgaatgatga gcacacacgt tttgattttg ctcattttgc
     9601 aaaagtttct gatgaagata tagccaaaat agaacaattg gtaaatgaaa aaattcgtga
     9661 gaatatagct ttagacgaac gcagaaatgt gcctattgaa gaagctaaat caatgggagc
     9721 tactgcatta tttggcgaga aatatggcga ctatgttcga gttatcatat ttgatcctgc
     9781 atacagtatg gagctatgtg gaggcacaca tgttaatgca accggtgaaa taggcttatt
     9841 taaaattcta agtgagtcgg cagtggctgc aggtgtaaga cgtattgaag caataacatc
     9901 aatagaggct gaaaaatata tcataaatca attgacacag ctaaacacga ttaaaacaga
     9961 actaaaaaat cctaaagata ttgttataag agtaaaggaa ctattgtcag aaaatgaaat
    10021 actgcaaagg caaatagacg gctttgtgca tgagaaagca cagctcatta aagctgacct
    10081 tctaaaaaat gtaaagttgc agaatggcat caatgttata gcacagaaaa ttgacttaac
    10141 taatgctgat gcaattaaaa acatttcttt tgaacttcgt aatcagatac ctgattcatt
    10201 tattttatta gcctctgaga atgatggcaa agccatgtta tcattgataa tttcagacag
    10261 tttggtgcaa tcaggcaact tgaatgccac aacagttatc agaaacattt ctaaacacat
    10321 tcaaggtggc ggtggtggac aaccatttta tgcaactgcc ggtggtaaaa atcctgcagg
    10381 aattgatgcc gcaattgaag aagcgttaaa gatgatataa aaagagcgag gctgcctgaa
    10441 tgggcagcct cgctctttta tatgttatta gaaattaata ttcccacatg tcgtgttcaa
    10501 gatttactat ctctgattta attctctctg cttcaagcaa agcatctaaa cctgttttgt
    10561 aatcttcaat acgtctgtta tacacatttg actctttaat aatatagctg ttaaacattc
    10621 gcttatgaaa cacgtcatca aatgttctgc gttctgtatc attttcacga ttgaatgctt
    10681 cagcatttgc aagcaacttt cttgcttccg gataataaat ccagaaaaga ggtttttgaa
    10741 tattaccttc tctgatattt ccatattcat cttcggcaag cattaggggt gcaataccaa
    10801 taatacggca ctccataact gaacgctgct tgtcaaagaa ccactcttct ttaatacgat
    10861 aggcaataac tttatcacgt gaaaaagtac gctgaatggc tgaatcacga gttagatatg
    10921 gaggctccgg atctgttatc tgaatagtat ccataccggc tccaccaatt ttcttgaatt
    10981 cttccaaggt cattggagta gtaaactcat catcagttgt agaataagca gttaatgaac
    11041 cctcatcaat tgcattcatc aacacctcta tcagactttt tctgtctttt gtatcgcctt
    11101 cacgggggaa acgaagtggc agattaatct tttgtttaag atctattacc tcccaaacac
    11161 gctttgccca caatacatca gcttcgcgca gaaatgaata ttccattact ttacgagtag
    11221 gattgtgttc tttaacataa acaccatcca ataccgtttg ggcatttgtt tgtttgacca
    11281 taaatgtcat cacaaacagg aaacaaaaca acaggacttt tttcatctta ccctccgtgg
    11341 tttattgtta aacgtattat tgaattgtaa agtttacact tggaatactt cttgtgccac
    11401 cgtccggacc aacagcttta atatattcaa aatatacttt atctccggca cgtgctttgc
    11461 ttaaagctgc tttcatatta gcagtaataa ggttgttatt gctttccaac tcaatcgggt
    11521 cttttccttt tggatataaa gacattttga attttgttac tttataataa atttcaaagt
    11581 cgaaattttc aagttcggca ataacggcac tttgcgcaat aagcgcattt ttctgcattg
    11641 ggccttcacg cttaccggca atttttgcaa ccggatcagg aactctttta acacgaaact
    11701 tttgtgctcc catattgatt ttcttaccat ccatatcagc aataatagaa atagttgctt
    11761 caccttgagt ggtaacttta acaatatagt cggcaccggt acctttcggg ttttttgtta
    11821 atgatccacc gccaccagat attgaggcta caactttatt gtttggtaca ccaggaactg
    11881 aaatcgaaac agggttttcc ggtccaatat agaatacgtt cattttggta ggagaaacaa
    11941 ctgcagcagg ttttgcagca atatattctg cttcaaatgg gtattctttt actttagttg
    12001 gatcttcagg gtctttaact ttgattactc caccccattt ctttacccct tctgatgatg
    12061 gtcggtcaac atatttacca agtccatttt ctacaggtaa agatcttcca ccaactacga
    12121 tttctgcatc agaactttta ttaaaagcag ctaaaaatac atctgcttta taatcctgac
    12181 cggttaatac atagcttgta ggggctacaa ctttaggttc tacatctgta aactttaaat
    12241 ccttagcatt aatctgcgcc aacaagtaat tgtttacttg tgattctgca gtctttacat
    12301 cagattgaat tttactcaac attgccaaag ctgcaacaac aggagtatgg taaaaggttg
    12361 ccatttccca tgttttcttc ttttcttctt tcgccagttt gctagctgga tccggatctt
    12421 taagattgat taactcatca aatcgagact gaaagtcttt cttatcctta tccgcaacga
    12481 aaccaatgag tttaaccttg taatcttcca gtttcttctt tagttctgtt gctttaaatc
    12541 ccttgccatc ttgtttatca ccacacataa ttaaagttgg tgtatcataa tcatcatttt
    12601 tctgaatttc acgaggagct ggaattttag cattcgcttc aagcttgtca gctttacgga
    12661 caatttcatt tttcaaatct tctaaatact tatacaaatc atcagaagct ttatgaacct
    12721 gagttgcttt ttcctgaaag ggtttcgctt ttacagcatc agcctgagcg gctgttgcaa
    12781 atgcatccat tacctggtta ttctttttaa taacgttatt gttggatgca gttattcctt
    12841 catcaatggt tataaaagca ttgatgattt cttttgaaac gttgagagca agcagagctg
    12901 ttaacacgag gtacatcatg ttaatcatct tctgccgcgg tgtcagttta ccactcgcca
    12961 tagtctattt ctctatatct aaaattggtt ggttataaaa taatttaatt agtcagatta
    13021 cttgtttgga ttcaggttca ttgctgatag catgtttcca taaacagtat taagagaaga
    13081 caggttttta gccaatttag aaacttcatc tttatacttc ttagtatcat ctaatgactc
    13141 gttcaagttc gacatcaatt ggttaattcc gtcataaaac ttagatgttt gttttaagtg
    13201 gttattagaa tcttgcaatt gaagttcata tacagcgttt agtgctccaa gatttttgcc
    13261 tgccatttgc acttgctcat tatacatttt aacgtcatca ctagcagttg acaaagagct
    13321 cattgcctgt gcagcctttt gatatgaacc tgaaagttct gttacagact tagtagctgc
    13381 atttacattt ttaacataat cattagtagc aacagatgca tcagaaagat cagccatttt
    13441 tgctgtatta tctcctaatg actttaaacc tgttcctaaa ctggcgatta aatccgggcc
    13501 aattttagca tcagataaca ttttatctaa agcctgtgaa acaggatcaa tttttttctt
    13561 tttatccctg ccttggcttg ggtcgtgcat tcctgctaac tccggataca ctaacgacca
    13621 gtctgtttct tcgtgaagtg gttcaaatgc agagaagaag aaaatgatgg cctcggtaag
    13681 aagacctact gtaagcatta tacctgcacc ttcccagtgc tggattttaa ataacgcacc
    13741 tgcaataaca atggctgcac caataccgta cagcttggcc ataaagtttt tgtacttttt
    13801 tcccgatgtg aattcagcta atcccatagt tgattttgaa aatttaattg gttgattttt
    13861 aatttattta ttttggtgat aataatcgaa ataatttatt cttaaaagtc ggctttatca
    13921 cgacccaaga atgacataac acaacggaaa cctacataac attttgcagt atcctgatac
    13981 tcgtaagaac gtgtgcctgt ttgtaaaaag tatcctacat ctttccatga accaccacga
    14041 attacttttc tttttaatgc aacaggatca gagtccttcg catcataaga ataatcaggg
    14101 ttaaggtcat gagagaaatt ataggcagct tcttcataag cattacttgt ccactcagaa
    14161 acgttacctg acatacagta taaaccatag tcattgggcc aataagatgt agtctttaca
    14221 gtgtaaaacc cgccatcatc catatagtta ccacgcattg gcttaaagtt tccaaggaaa
    14281 catccacggc tgtttctgat ataaggtcca ccccatggat aaggagctaa atcatgtccg
    14341 cctcttgctg cgtactccca ctcggactct gtaggtaatc taaaatcctg aacaaatgat
    14401 tcaccatttt cctgcaagta gctatttaat aattgtgttc tccagataca gaaagcacgc
    14461 gcttgcttcc aggttacacc tacaacagga taatcgtcat aagccggatg ccagaaatac
    14521 atgttagtaa caggatcatt gtaagaatat gtataatcgt gtatccatgc taatgtatca
    14581 ggataaacat tgataatatc tttcttaata aatactgaac gatcagtcat accttgttca
    14641 cggtttgact tttgagctgc ttctttaagg tcaatccagt aatattcgaa attcaattta
    14701 cgtgaatcaa tttcttttct tctgtaaaag cgctcacttt ccggtaaaaa caactcagct
    14761 aaggtttcta cgttctgttc atcttcccat ttgattttct tacgccaatt gatgcgttcg
    14821 ccatattctc cttcttcgat aaggtgatct ccaccgatta atctgtgtgc aagcgagtcg
    14881 cgaacccact caacaaactg acggtactca ttgttggtaa tctctgtatt gtccatataa
    14941 aatgcatgaa cagaaacaga tttactctgt gctaccattg cgtaaggaac atcctgatcg
    15001 cttggcccca tattatatgc gccagatggt ataaacacca taccgtatgg atcagcctga
    15061 taccagcgct ctcggttaag agcgccaact aactgtcctt tgtttccgcc accgcagctg
    15121 ctgagtaaaa taactgcaat gatgggaatg aatagttttc tcatgtttgc caaattagta
    15181 actggagaat tatggatgtt aaaacgattt atatttattt ttattacttt aagtctgtac
    15241 tacgctgttt tattattctg tttatacttc agtttcaaag ataagttact attaaaatta
    15301 agtttcccga aatttataaa aaccttacat ttcttaatac aggaattact tttggtttaa
    15361 tcacataaca ataacctaac attatttcat gtgttccgct atgatagccc tttaattttg
    15421 aggtagtaat atcataagag tatcctaatc tcaggttttc gataatatta atacctgcca
    15481 ttaaaacaat tgcatcctga agtctgtaag atgctccaat ccaaacttta tctttaatat
    15541 gcagattggc atttatatca gcctgtgttt ctttagcatc tgactttata aataaagatg
    15601 gttttaattt caaagatggt gtaagatcaa agtcaacacc tcccataaaa taaaaatgag
    15661 gtacaacttc tactttggca tcatcggtat attcaatttt tcctcctgtt aaatgtgtgg
    15721 ccgaagcacc tacataaaat ttatctgatt tataatataa accagcacca aggtcaaact
    15781 tagagcctgt tcctgatttt accaatgggt cgtcagggtc aatagcatta aagtcgtttc
    15841 cgtaaccacg ctgtaataaa ccggcatcaa gtccaatccc taatttacct gaacccaggt
    15901 ctaaatggta tgaatatgcc agtttgatgt taagattttt ttgattcaat ggcttatcag
    15961 ttaaaactga aagaccaagt ccgccatgta aaacttctac aggtgattct actccaaaaa
    16021 cagcagtttt tggtccacca ccaaatttat cccattgatc gcggtatatt aaagtaccac
    16081 aaattgcttc attggtacct gcagatgccg gatttggata caagcggtta aacatgtttt
    16141 gtgaaaattg aggatcctgt tgtccaaagg atactgatgc ggttgccaca gctacgactg
    16201 taaaaagtct cttcatatac tattcaggtt gttgattcgt ttaatattat acgtcttaat
    16261 ttgcggccct aaagtaagca aaaaaacaaa ttgcaaacaa aagacacttt tttatttcaa
    16321 taaatgcgtt aaaatcgggc atagttacga cagtttcttt aaagcttgcc accatctttc
    16381 cggaatttcg ttgttacaag ccttctgata atcatcataa ctgcaaggaa ctaactgatg
    16441 cctgtctaat ttctttttaa tcttactcag aggtatttcc atccaccaac gtcccgttac
    16501 attacttttc agaaaatgca tttcttcgtt gttctctctc agtgtcaaat tataaactgt
    16561 aaactgagag cttgtgctat caggaaaatc attctttctg ttgcttacac cttccataaa
    16621 ataccacacc atttgagaaa ttaatgctgc tgtcatttgg tttgtatcta ttgaggctgc
    16681 atactcataa agtccaattc cttctaattt atcactcata cctgcgtaat acattatctg
    16741 acaggccata tcgcctgtta aaccgttagg tgatggcgtt gaagtagctg aggcatc
//
LOCUS       DAOOOL010000046        16567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_286_length_16567_cov_70.969901, whole genome shotgun sequence.
ACCESSION   DAOOOL010000046 DAOOOL010000000
VERSION     DAOOOL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_16567_cov_70.969901"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKU82_05510"
     CDS             complement(<1..456)
                     /locus_tag="PKU82_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82366.1"
                     /translation="MKNQIVAILLVCCLIQTNMCLAQQNKYLDSLKYSLNHAKADTHK
                     LNLYQKLIYYLLDFNTDSALFYSKPALQFADSLKWEPGTKKLVALVGRVYWRKGFFDM
                     ALKCHFDVLKYYESRNDEPQIAELLTFIGQDYADGGRYEDALSYFKKAGA"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <721..2489
                     /locus_tag="PKU82_05515"
     CDS             <721..2489
                     /locus_tag="PKU82_05515"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82367.1"
                     /translation="YEKLRDSSKMGGNYLLFGFVYESQGNYVENVAANYKALKIFEAI
                     GDKYGEAIVLSNIAGVYSTQGNYSEALKLYLSAIKAFDKAKDIINLTVAYTSVGQTYQ
                     QLGNTTEAISYYNKAYEIAKSISNDFAMATASESLGDVYFQKQNYQKAIQFFQAAANS
                     FRVVSDYFDLASALSKLSICQTILGNYDSAKKNLDEAWTLAEKLDSKVPVTEYYHAVA
                     LLDSATSDWQGAYNNYKNYIILRDSTYNRENTKKILLQQIQYENNKKDALLKAEQEKN
                     AVAQRVFRNSMIAGASILLLLLMLLFHRYRFKQKTNLQLKEAYQHLQATQQQLVQQEK
                     LASLGVLTAGIAHEIKNPLNFVTNFASLSNEILIELKEAQTVQDKDEAMNLLHENLVK
                     ITEHGHRADNIVKRMLEHSRTNTEDRQKTNINSVCDEYLDLAYHGKRAILQNFNCDLI
                     KNFDPNLPQVICNGQDISRVVLNLIDNAFDELYECERLKLKGENFKPQLVITTASIPQ
                     TSRQKSNRKIFISVRDNGRGISEKMKQKIFEPFFTTKPTGKGTGLGLSLSFDIINTHK
                     GTIEVTSKELEGTEFKIILPVS"
     gene            complement(2662..3279)
                     /locus_tag="PKU82_05520"
     CDS             complement(2662..3279)
                     /locus_tag="PKU82_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82368.1"
                     /translation="MLNRFFSVVTYLTHPLLIPTYLLLILFNSDTYLSYTLSPALQRI
                     ILSAVFVLTCLMPFLSSVLLLKKQGVQGLLMEDRQSRTIPYFFTLIYYVACYYFLLKL
                     PVSSVIKILILAAACCIALAFTINFFWKISIHLIGLGGMAGAFYVLSLLLSSEFILPF
                     VLSIILAGLTASARLMKNAHSPSQLYFGFIAGFVCEYIFIMYSLK"
     gene            complement(3282..4810)
                     /gene="rpoN"
                     /locus_tag="PKU82_05525"
                     /pseudo
     CDS             complement(3282..4810)
                     /gene="rpoN"
                     /locus_tag="PKU82_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
     gene            5032..6879
                     /locus_tag="PKU82_05530"
     CDS             5032..6879
                     /locus_tag="PKU82_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOZ82369.1"
                     /translation="MTEVNNDFHADENQQAGEIQSENASITQPLQEEHLATLPEEHPE
                     TLPEQEENYNHLTKEELVKIAEGFAEGDVAAVKSKINALKNAFEQIQSSEKEQALSTF
                     LEEGGVKEDFKYNYTQSDDRFQAALKKYNKRKAEYAEQQEKLRENNFSVKQEILVAMK
                     NLIQNEENMQRAFDQFHELQAKWRATGPVPSGQLNDLWMTYKLYTDKFYENLKINREL
                     QELDFKKNLEAKIELCEKAEDLINEPSLNSALQTLHNLQNKWRETGHVGREKSNEIWE
                     RFKAASDKVYERRKEYFEQLKQRYAENLTAKNLLITKTEELLKDEPLKASEWMEKSKM
                     LVEVQNEWKKVGFADKKSNDEVWAKFRGLCDGFFVRKNDFFTSLKKEHAANLQLKNEL
                     CIQAEALIENTDWKKTTEELKRLQAEWKKTGFAGDRNNQKIWERFRKACDSFFERKNA
                     HYTSLESDYKENFDKKNALIEKAEQFTLGENIDESVEQLKDFQRQWSEIGMVPLEHKD
                     TIYAKFKAAIDKHFQTIRQHNKERFTSGHKPSFRSGKPGNEKNDLKHKINQLTGEINT
                     WENNLGFFARSKNADAVRKEFEDKINHAKEEIKRIKKQLDEISQTPKEQ"
     gene            complement(6904..7467)
                     /gene="frr"
                     /locus_tag="PKU82_05535"
     CDS             complement(6904..7467)
                     /gene="frr"
                     /locus_tag="PKU82_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOZ82370.1"
                     /translation="MNDIAKKIIDTMQSHMDKSISHLETELAKIRAGKANPQMLESIH
                     VDYYGSQTPLHQVANVNTPDARTIVIQPWEKGMLGPIEKAIQIANLGFNPQNDGSIIR
                     IAVPPLTEERRKDLVKRCKAEGENAKISLRSIRKEANDAIKKELKKGITEDEAREAET
                     KIQQTTDGFISRVDKHLETKEKEVMTV"
     gene            7559..7882
                     /locus_tag="PKU82_05540"
     CDS             7559..7882
                     /locus_tag="PKU82_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOZ82371.1"
                     /translation="MRFQKHIFVCTNQRKEGERKCCGEVHGLALVAAFKKKIKELQLP
                     VPIRAQRSGCLDACDYGPSVVVYPEGIFYGNVQLQDVDEIVEQHIKNNKPVERLIINF
                     DDKKV"
     gene            complement(7896..8249)
                     /locus_tag="PKU82_05545"
     CDS             complement(7896..8249)
                     /locus_tag="PKU82_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOZ82372.1"
                     /translation="MDIEKFRNYCLSKQETTESFPFDEDTLVFKVAGKMFALVSLSEK
                     PMTVNLKMDPEIISEWRDRYSEITPGFHMNKQHWNSVIFNGSIPLAELKWLIDHSYSE
                     VVKKLPKKVRERFTI"
     gene            complement(8334..9797)
                     /locus_tag="PKU82_05550"
     CDS             complement(8334..9797)
                     /locus_tag="PKU82_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82373.1"
                     /translation="MDILRKTIKKLSDEEYQALLLQVSGKKKNKPYYVLEATRNNNHF
                     DDTEMMKQLQVNPSAYYTLKSRLNTRIAEILSKKVENPIRELMEEVVRVPATLYGTNR
                     DFSIRALKEIEKQLKEYDMSNELITVYKTLALLHIHHEDYEHYNKLYNKHVAYALAIS
                     KAEQMFFLFFKKLGTYQLTLDPDTLEEVIMLKRKIANIAEMYDSHRLFVLYNIVRIYY
                     LCSLPSKRQELKSLELEIESILKRINEIFNAFPIDTLYSNISPLVDILHYEYYQKIQN
                     QVRADHYLSNGINDTIIDWSEKTAMHFFIIQYLNSRVEKFLADGEMYRLNEFNQTIEK
                     TFDLNINEPYHYISFKRYIALCKFYSKDYQGAAKTIYDLRNNISLKQFTHTDVDSKLF
                     QGLCYCLMGEDSLAQQMISSIKRQIADFEHEFESTTIFIKILKTALKPSDFRRKIVRI
                     NELLIKFEEANNGDKPILKYIKIDDALIRKMTNPIKD"
     gene            10184..10768
                     /locus_tag="PKU82_05555"
     CDS             10184..10768
                     /locus_tag="PKU82_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82374.1"
                     /translation="MEDTTMNNTMDFYKMVRENRFAMYSKPRRYYNRDGIKGSAIKFL
                     FFVQAIIFFLMLVTLHSATAQTANTQPALMADANLNGTIANYTTIYNSGKVFLNWTAK
                     NEAADCLYIVERSLDGKVYESVGIKEGLGTAVELFYSWMDQTPPNGYAFYRVKKIARD
                     GSQYYSSTNTVINLDSSYDPKSNYASDKKPEDQK"
     gene            10820..>16567
                     /locus_tag="PKU82_05560"
     CDS             10820..>16567
                     /locus_tag="PKU82_05560"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82375.1"
                     /translation="MNRYFTTGRNITWKLCFAITLLLGTFNAKAQIPTASFAADSTEG
                     CVPLTVHFSNTSTLATSYFWDFGNGNTSTLANPQTVYLAPGNYTVKLIAYNSTSGQSD
                     TLIIPNYIDVVSFPTANFTSNVTSGCKPLSVVFNNTSSNFTSCIWDFGDGFSSTQTNP
                     THTYSNAGTYTVKLVAYSSYGCSNVKTQTNYITVYGQPTAQLTANPSSTCDANQVIQF
                     NSSSAGVVSYLWNFGQPSSGGANSSTLQDPTHTYGTQGSFNVSLIVTDANGCSDTAVI
                     NNMITIGTALTPTFTWTNGAGCKPLTTNFTAPSITGATSYAWDFGDPSSGVQNTSTSA
                     NPTHTYANSGTYSVSLYVTTSSGCNGGVTLNNIIVVDQPPVAAFTSNPTTGCKPLAVQ
                     FNNTSTGAATYLWEFGDGTTSTLASPSKIYTTAGTYNVTLHAFSTNGCETIIVKNNYI
                     VVNEIKSSYYFLPGSGCAPLSVNFIPTNNSGFVSYEWNFGDPASGALNTSTLSNPTHI
                     YNNIGTYSTKLIVTSAAGCKDTLIKNFVKVRASSVVYTVPDTIKGCQPYPVSFTNPLP
                     GATVFHWDFGVPWLTNDTSNIANPSFTFDSVGIFNVTLNAYMPSSGGAGCSQFFNPVA
                     IVQVFPMLISPITYIQATPCAPYLIHFSDTTMDVVSWDWDFGDGTPHATVQFPSHTYT
                     QPGTYTVVLNIVLQSGCMTSLSTTITLGYPNPITVNKKAGCHDEPFTFGLTGGPWTNV
                     QWNFGDGVGTSTSPNPTYTYNQVGTFTVSVNVTSSDGCVNTYTDSITTTNPQPSFVIN
                     GPTSSCTNQFVFFTNTSTGATSYWWDFGDPTSSTGFSSLVNPSRNFTTPKAYTISLTA
                     TNGACSRTVTYPALIRINRAAPNFSFTQSSQCFPITVTLTDLTGPTPAIQWGWNFYNG
                     STDSTQNPVYTFNAPPTSDTISLWVRDTNGCSATKRLKGIKYKQAKFTMSATGGCKPS
                     TICFTDASDTTVVSRKWDFGNGATSTQTNPCYTYTTDGVYTVTLITTFSTGCKDTLVM
                     PNAVTISTPVADFSVPTQAFCAPALVNFNNLSTNATTYYWDFGDGSNSIATTPSHVYN
                     VPGDYTITLIAINGTCRDTMKKVNYLHVPGTFSYFNLVSLQSCVGNLVQFTDSSLNAQ
                     TWLWNFGDGDSSTLQNPTHLYSTTGSFTVSLITSDNLGCSSHYIFSNPIVVHPVPTAV
                     ANTIDTVGCSPLTSTFTQNSINATSYLWNFGDPASGALNTDTAPTSTHTYNNAGNYQP
                     YFVAYNNFGCSDTMWLPKTINVNQTPVAQFSSSATNGCTPLTVNFTNTSTKLNTPQYL
                     WNFGNGNTAITSNATAVYADSGNFNVSLIVTNAAGCADTATAQITTVLSPTAIASTTD
                     TVGCSPHTVNFTNISLNATSYLWNFGNGVTSTLANPSYTYNNAGNYTVTLIATNASGC
                     SDTLVFPSQIIVNQTPRARFNMSPATGCSPLTVNFTNTSANTSGATYSWNFGNGNTAS
                     TANTSSVYPDSGTFAVTLLVTNGNGCSDDSVRNVVVNLSPTASATTNDTTGCNPYTST
                     FVNNSINATSYNWNFGDGGTSTQVNPTHTYPGTGNFTVTLIATNTAGCKDTFVFNNTI
                     QVNQTPLASFTSSATTGCAPLNVNFTNTSTVISGATYFWDFGNGNTSTVANPSTVFAD
                     SGTYQVVLNVNNGNGCTHTASKYITVNITPTASFATTDTSGCTPHAVSFTNNSTNATS
                     YQWNFGNGATSTAVSPNYTYTTGGLFTVTLIASNSAGCRDTFVLPYQINAKQSPRARF
                     NMSPTSGCTPLNVNFTNTTANMQSPTFNWNFGNGSSATIANPSTVYSDSGTYAVTLTV
                     TNSNGCFDDSTRIVRANLSPTAIAATTDTAGCAPYTSTFTNSSLNATSYQWNFGDGGT
                     STVATPTHTYSTSGNY"
BASE COUNT         5469 a         3087 c         2874 g         5127 t
ORIGIN      
        1 agcacctgct tttttaaaat aggataaggc atcttcatat ctgccgccat ctgcataatc
       61 ctgaccaata aaggttagca attccgctat ttgtggttca tcatttctgc tttcataata
      121 ttttaaaaca tcaaaatgac attttaaagc catatcaaaa aaacctttac gccaataaac
      181 cctgccaact aaagcaacta atttttttgt tcctggttcc catttcaaac tgtcggcaaa
      241 ttgaagtgcg ggtttactat aaaaaagggc agaatcggta ttgaaatcta ataaataata
      301 gattaacttt tggtaaagat taagcttatg tgtatccgct tttgcatgat tcaatgaata
      361 cttaagggaa tctaagtatt tgttttgttg tgccaaacac atattggttt gtatcaaaca
      421 acacactaac agaatcgcaa caatttggtt tttcataaag acgattcacc aaatttaaag
      481 gtctgttgtg tacaaattac ttttttgcaa tttttttttt ggaattgaag tagggaaaac
      541 aactgttaca gaaatagttt ttctaaaatt ccggtataag cgaaataata gtttgataca
      601 gtaatactat tgtggaatga ataaaatctg aattagaata tttaaattca tctataggac
      661 aattttggca cagcagttga atttcatcag gtaaattaga aagtgaggtt nnnnnnnnnn
      721 attatgaaaa gcttagagac agcagtaaaa tgggaggaaa ctacctttta tttggttttg
      781 tgtacgaaag tcagggtaat tatgtggaga atgttgctgc aaattataaa gcacttaaaa
      841 tatttgaagc tattggtgat aagtatggcg aagctattgt actgagtaat attgccggag
      901 tgtattccac tcaaggaaat tattctgaag cattaaaact ttatttgtcc gccatcaaag
      961 catttgataa agcaaaggat attattaatc tgacagtagc atataccagt gtcggccaga
     1021 catatcaaca attgggtaat acaacagagg ccataagtta ttataataag gcgtatgaaa
     1081 ttgccaaaag tattagtaat gattttgcca tggcaactgc ttcggaaagt ttgggcgatg
     1141 tttactttca gaaacagaat tatcaaaaag caatacagtt ctttcaggca gcggctaatt
     1201 cttttagagt agtatctgac tattttgatc ttgcctctgc tttaagcaaa ctaagcattt
     1261 gtcaaactat attaggtaat tatgattctg caaagaaaaa ccttgatgaa gcttggactc
     1321 ttgcagagaa attagattca aaagtaccag taacggaata ctatcatgct gttgccctac
     1381 ttgacagtgc aaccagtgac tggcaaggcg catataacaa ctataaaaat tatatcatcc
     1441 tgagggatag tacctataac cgcgaaaaca ccaaaaaaat tctgctgcaa caaattcaat
     1501 atgaaaataa taagaaagat gcactcttaa aagctgaaca agaaaaaaat gctgttgcac
     1561 aaagagtttt cagaaattcg atgattgcag gtgcttcaat tctattgctt ttattaatgc
     1621 tactgtttca caggtatcgg tttaaacaaa aaacaaattt gcaattgaag gaagcatatc
     1681 agcatttaca agccacgcaa cagcaattgg tgcaacagga aaaacttgct tcactgggtg
     1741 ttttaacggc aggcattgca catgaaataa aaaatccgtt gaattttgtc acgaattttg
     1801 caagtctgag caatgaaata cttatagaat taaaagaagc tcaaacagta caagataagg
     1861 atgaagccat gaatttgctt cacgaaaacc ttgttaaaat tacagagcat ggccaccggg
     1921 ccgataatat tgtaaaaaga atgttggaac attctcgaac caatacagaa gaccggcaaa
     1981 agactaacat aaactctgtg tgtgatgaat atttagatct tgcttatcat ggtaaacgtg
     2041 ccatcttgca gaattttaat tgtgatctga ttaaaaattt tgatccgaac ttgcctcaag
     2101 tcatttgcaa tggtcaggac atttcgaggg tggtacttaa cctgattgat aatgcttttg
     2161 atgaattata cgaatgcgaa cgtttaaaac ttaaaggtga aaattttaaa ccacaattgg
     2221 ttattacaac ggcttcaatc cctcaaacat caaggcaaaa atccaatcgg aaaatcttta
     2281 tttctgttcg tgataatgga agaggaattt cagaaaagat gaaacaaaaa atattcgaac
     2341 cattttttac aactaaaccc acgggtaaag gaacaggttt aggactgagt ttaagctttg
     2401 atattattaa tacacataaa ggcaccattg aagtaacttc aaaagaacta gaaggcacag
     2461 aatttaaaat catcttaccg gtttcataat gtacaagagg gcttacactc ttcattaaca
     2521 tgtacactta ctactgagga tcagttgctg atggtttttc cagtttttga tatacacttt
     2581 tagtcgttcg gtggtataaa aaattaaaaa acgaatttaa ataatttgag attttatata
     2641 tgaattttag aaggttgtag tttattttaa tgagtacatg ataaaaatat actcacaaac
     2701 aaaacctgca ataaacccaa aatataactg acttggagag tgtgcatttt tcatcaaacg
     2761 ggcagaagcg gtgagtccgg caagaatgat tgatagtaca aacggcagaa taaattcact
     2821 acttaacaat agcgacagta cataaaaagc acctgccata cctcctaatc caatgagatg
     2881 aatacttatt ttccagaaaa aattaatagt aaatgcaagt gctatacaac atgctgctgc
     2941 cagaataaga atttttataa ccgatgaaac cggaagtttc aacaaaaagt aataacaagc
     3001 tacataatat ataagtgtaa aaaaataggg tattgttctg ctctgcctgt cctccatcaa
     3061 caaaccctgc acaccttgct ttttaagtaa taataccgat gataaaaaag gcatcagaca
     3121 ggtgaggaca aaaaccgctg ataaaattat tcgctgtaat gcaggcgaaa gggtatatga
     3181 cagatatgtg tccgaattaa aaagtatcag taataaataa gtaggtataa gtaaaggatg
     3241 ggttagataa gtgacgactg aaaaaaagcg gttcagcatt tttaaaactc tttacgtagc
     3301 ctggcaacag gaatatttag ttgctcgcga tatttagcaa tggttctacg cgccacatta
     3361 taacctttgt cttttaaaat ttttgccaat ctgtcgtccg gcaatggttt tttcttgtct
     3421 tcaccttcaa tagcatctga cagaattttt tttacttcgc gcgtacttac ttcctcgcct
     3481 gagtctgttg ccataccttc tgagaaaaaa gtttttttat cagaaatgtg ccgaatggtg
     3541 tttgcacata tttactgttt gctactcgtg aaacagttga aatatcaagc ccgacaacat
     3601 ctgcaatgtc ttttaaaatc attggcttta aaagtcgttc atcgccttcc aggaaatatt
     3661 ctttttgata gttcatgata gcttgcatgg tatgaagcag tgttgcctgt cgctgtctga
     3721 tggcatcaat aaaccatttt gctgaatcaa ttttttgttt cagataaagg atagcctctt
     3781 tctgtgaacg atcttgcttt ttactcttgg catagccttc catcatttca ttgtactcgc
     3841 ggttaattcg caaatctggg gcattgcgtg agttcaatgt caattcaagc tgacccacgt
     3901 tatttgtaat gataaaatcg ggaacaatat gttgaacaga ttttgtttct gcagaaatat
     3961 caccccccgg acgtggattt aattttaaaa tttcattcat ggcctccttc aaatactcat
     4021 caggaatgtt catcaatctg gcaatttttt caaaatgctt ttttgagaac tcatccatgt
     4081 aatttttaat aatttcaatt gccctattaa ccgacttact attagatttt cgctgcaatt
     4141 gaagcaaaag acactcttgc aaattacgag cgccaacacc gggtggatca aatgtctgta
     4201 tcacttgtaa cacttgtaaa agttcatcgg cattagtatc tatattctgt gtaaatgcca
     4261 aatcatttac aatagaattc aaatctctgc gtaagtatcc atcttcatcc aaactgccga
     4321 tcagatgctt agccatctgc atttgttttt cgtttagggc aaccataccc aactgtgcca
     4381 gtaatgtttc ctgaaatgtt actgaagacc cataaggaac ctctgtgcgt tcatcatcgg
     4441 gcgaagtatt attggcagat gttttataat atggtatatc ctcatcagtg atataatcat
     4501 ccacatcaaa atcatcatct gttctttctt ttatatcatc atcagaagtg ttatcaaact
     4561 cgtctttttt attctcctcg ccttcattgg cttctacccc ctcttccaat tcaggcactt
     4621 cgtcctcatc ctgttgtgta tcttcatcca catcttcggg cgcaccttct tctaatgcag
     4681 ggtttacttc catttcctct tttatccgct gttctaatgc aatagttggc aattgaagca
     4741 atttcatcag ctgaatttgc tgaggagaaa gtttctgtaa aagtttttgt gataagtttt
     4801 gttttaacat aattcaataa cgctccaaat ttaccatttt ttatacgtgt agccggcaaa
     4861 aatggttttt atggaattac aatgtggaat aaatctaatt ttcaaaaagg cttttttaga
     4921 gcaataactt cttgcacaga aatggaaaat tgtattacat ttgtagtctg ataaagcctt
     4981 ctacaatgga aggcttcatt gtttgatttt aatattatat taattagtat catgacagaa
     5041 gtaaacaatg attttcatgc ggatgaaaat cagcaggctg gtgagattca atcggaaaat
     5101 gcatctatta cacaaccctt gcaggaggaa catcttgcaa cattgccaga agaacatcct
     5161 gaaaccttgc ctgagcagga agaaaattac aatcacttaa ccaaggaaga acttgttaaa
     5221 attgctgaag gatttgctga aggtgatgtt gctgccgtta aaagtaaaat caatgcactg
     5281 aaaaatgcat ttgaacaaat tcaatcttca gaaaaagaac aggcactatc aacttttttg
     5341 gaagaaggtg gtgttaaaga agatttcaaa tataactaca cacaatcaga tgatcgtttt
     5401 caggcagcac ttaaaaaata taataaacgc aaagctgagt atgccgaaca acaggaaaaa
     5461 ctcagagaaa ataattttag tgtaaagcag gaaatacttg ttgccatgaa aaaccttatt
     5521 cagaatgagg aaaacatgca acgcgcattc gatcagtttc acgaacttca ggcaaagtgg
     5581 cgtgcaacag ggcctgttcc ttcaggtcag ttgaatgatt tgtggatgac ctacaaactt
     5641 tacacagata agttttatga aaacctgaaa attaaccgtg aacttcagga acttgatttt
     5701 aaaaagaacc ttgaagctaa aattgagctc tgcgaaaagg cagaagacct gattaatgag
     5761 ccatcattaa attcagctct gcagacattg cataatttac aaaacaaatg gcgcgaaacc
     5821 ggtcatgtag gtcgtgaaaa aagcaatgaa atttgggaac gatttaaagc agcaagtgat
     5881 aaggtttatg aaagaagaaa agaatatttt gaacagctaa agcaacgcta tgctgaaaat
     5941 ttaactgcaa aaaatctact tatcaccaaa acagaagagt tacttaaaga cgaaccactt
     6001 aaagcatctg aatggatgga aaaaagcaaa atgcttgttg aagttcagaa cgaatggaaa
     6061 aaagttggct ttgccgacaa gaaatctaat gatgaggttt gggcaaaatt cagaggattg
     6121 tgtgatggtt tttttgttcg taaaaatgat tttttcacct cactaaaaaa agaacatgct
     6181 gccaatctgc aattgaaaaa cgaactgtgt attcaggcag aagcattgat agaaaacacc
     6241 gactggaaaa aaacaacaga agaattaaaa agacttcagg ctgagtggaa aaaaaccgga
     6301 tttgccggtg atagaaacaa tcagaaaatc tgggaaagat tcagaaaagc atgtgattca
     6361 ttttttgaaa gaaaaaatgc acattacact tcacttgaat cagactataa agaaaacttc
     6421 gacaagaaaa atgcactcat tgaaaaagct gaacagttta cacttggtga aaacattgat
     6481 gagtctgttg agcagttaaa agattttcaa cgtcagtgga gtgagattgg tatggtacct
     6541 cttgaacaca aagacacaat ttatgcaaag ttcaaagcag caattgacaa acattttcaa
     6601 acaataagac agcataacaa agagcgattt acatcaggac ataaaccatc ttttagaagc
     6661 ggcaaaccgg gtaatgaaaa gaatgattta aagcataaga ttaatcaact taccggagaa
     6721 attaatacat gggaaaataa tcttggcttt tttgcacgct caaaaaatgc agatgctgta
     6781 cgcaaggagt ttgaagataa aatcaatcat gccaaagaag aaataaagcg tattaaaaaa
     6841 cagttggacg aaatttctca gactcctaag gagcaatagt aactactgac tatttagaag
     6901 gcgttaaaca gtcattactt ctttctcttt tgtttcaaga tgtttatcca ctcttgaaat
     6961 aaagccatca gttgtttgct gaatttttgt ttctgcctcg cgtgcttcgt cttcagtgat
     7021 gcctttcttc aattctttct taattgcatc attggcttct tttctgattg aacgtaaact
     7081 tattttagca ttctcacctt cggctttaca acgtttaacc aaatcttttc ttctttcttc
     7141 agttaatggt ggcactgcaa tacgaatgat agaaccgtca ttctgcggat taaatcctag
     7201 attagcaatc tgaattgctt tttcaatggg acctaacata cccttttccc atggttggat
     7261 tacaatagtg cgggcatccg gagtattgac attggcaacc tgatgtaatg gcgtttgcga
     7321 accatagtaa tcaacatgaa tagattcgag catctgtgga tttgctttac cggctctgat
     7381 ttttgctaat tcagtctcaa gatgcgaaat ggatttgtcc atgtgcgatt gcatcgtatc
     7441 aattatcttt tttgctatgt cgttcatact ctgtaatatt taacagcaaa attaagcatt
     7501 gaatttgaat tggactaata gagtttgaat tctgaacaca attatcttaa tttgcattat
     7561 gaggtttcaa aaacatattt ttgtttgtac taatcagcgt aaagaaggtg aacgtaaatg
     7621 ctgtggtgaa gtacatggtc ttgcattggt tgcagcattt aagaaaaaaa ttaaagaatt
     7681 acagttgccg gtgccaattc gtgcacaaag gtcaggttgt ttagatgcct gcgactatgg
     7741 tccatcagta gttgtttatc ccgaaggtat tttttatggt aatgttcagc tacaggatgt
     7801 tgatgaaata gtagaacaac atataaaaaa caacaaacct gttgagcggc taattataaa
     7861 ttttgatgat aagaaagtgt aatttctgaa taacactaaa ttgtaaatcg ctctcttact
     7921 tttttgggta attttttaac tacttcagaa tagctgtgat caataagcca ttttaattct
     7981 gcaagaggaa tggagccatt aaaaataaca gaattccaat gttgcttatt catgtgaaat
     8041 cccggtgtaa tttcggaata gcgatcacgc cattctgata taatttcagg atccattttg
     8101 aggtttaccg tcattggttt ttctgataac gaaactaaag caaacatttt tccggcaacc
     8161 ttaaacacaa gtgtgtcttc gtcaaaagga aaactctctg ttgtttcttg cttcgacaaa
     8221 caatagttcc tgaatttttc aatatccatt tgatagtatc agatgataaa agtaacagaa
     8281 atttgtcaga aaaagcacaa aaataaaagg cggccatttc tgccgccttt gttttaatct
     8341 ttaatcggat ttgtcatttt ccgaatcaat gcatcatcaa ttttgatata cttcagaatt
     8401 ggtttatcac cattatttgc ttcttcaaac ttaatcagca actcgttgat gcgcacaatt
     8461 tttcttctga agtcagaagg ctttaatgct gttttaagaa tcttaataaa tattgttgtt
     8521 gactcaaact catgttcaaa gtcggcaatc tgacgtttaa tacttgaaat catttgttgt
     8581 gcaaggctat cttcacccat caggcaataa cacaaaccct gaaagagttt gctgtcaaca
     8641 tcggtatgag tgaactgctt caaagaaata ttatttctta aatcgtatat agtttttgct
     8701 gcaccctgat aatctttact ataaaattta cataaggcaa tgtatcgctt aaaagagata
     8761 taatgataag gctcgttaat attgaggtca aaagtttttt caattgtctg attgaattca
     8821 ttcaaccggt acatttcacc atcagcaaga aatttttcaa ctcgcgaatt cagatactga
     8881 atgatgaaaa agtgcatagc tgttttctca ctccagtcaa taatagtatc gttaattcca
     8941 ttactaaggt aatgatctgc tctaacctga ttctgtattt tctgatagta ctcatagtga
     9001 agtatatcta ccaacggact aatgttgcta taaagtgtgt caatcggaaa tgcattaaat
     9061 atttcattga tgcgttttaa aatactttct atttcaagct caagggattt taactcctgt
     9121 cgttttgatg gcaatgaaca cagataatag attctgacaa tattgtataa cacaaacaaa
     9181 cgatgcgaat catacatttc tgcaatattg gcaatctttc ttttaagcat tatgacttct
     9241 tccaacgtat caggatcaag tgttaactga taggttccta attttttaaa gaacagaaaa
     9301 aacatttgct cggcttttga aatagccaat gcataagcaa catgtttgtt ataaagtttg
     9361 ttataatgtt cgtagtcttc atgatgaatg tgtaacaatg caagagtttt ataaactgta
     9421 atcaactcat tgctcatgtc atattcctta agctgctttt caatttcttt taatgctctg
     9481 atagagaaat cgcggttggt tccataaagt gtagccggaa cacgaaccac ttcttccatc
     9541 aactcgcgaa ttgggttttc aactttcttg gaaagaattt ctgctattct tgtattgagg
     9601 cgggatttta atgtataata agcacttgga ttaacctgaa gctgcttcat catttccgta
     9661 tcatcaaaat gattgttatt gcgtgtagct tctaagacat aataaggctt gtttttcttc
     9721 ttgcccgaga cctgaagcaa caatgcctga tactcttcat cactaagttt cttaattgtt
     9781 tttcttaata tatccatata agcttatgat ttatttttta aagactcttc cttgttacgt
     9841 aaaaaaaact gcaaggtttt agctttttta aaaaatttcc gacttagcta attactctaa
     9901 ttgcataatt gaaactcaat attaatccat ggcatatatg taatactaaa tatacttttc
     9961 tgtaaatcag cttatgtata acaataaaag tactactaaa aagcacggtt tatgaagcaa
    10021 aataatatca atttattgaa aagtgtaacc cggattcaaa atgggcgtaa aagcggatat
    10081 gaaaagaaaa tgtgcagtta tattacattg taatgtttta taagaaatta caaaaaccac
    10141 tgctcatata aaacgtaaat ttcacttata aaaatatatc attatggaag atacaacgat
    10201 gaacaacaca atggactttt acaaaatggt tcgtgaaaac cgttttgcca tgtattcaaa
    10261 accacgcaga tattataatc gcgatggcat aaaaggttca gcaattaagt tcttgttttt
    10321 tgtacaggcc atcattttct ttttgatgct tgtaacactt cattctgcaa cagcgcaaac
    10381 agctaataca caacctgcat taatggctga tgccaactta aatggaacta ttgcaaacta
    10441 tacaaccatt tacaattcag gaaaagtatt tctgaactgg acggctaaaa acgaagcggc
    10501 tgattgtctt tatatcgttg agcgctcact tgatggaaaa gtatatgagt ccgtaggtat
    10561 aaaagaaggt cttggtactg ctgtagaact attctactca tggatggatc aaacaccgcc
    10621 aaacggatat gctttttaca gagtaaaaaa gattgctcgc gatggctcgc aatattacag
    10681 ttctaccaat acggtaataa atcttgattc gagctatgat ccgaaatcaa actatgcttc
    10741 ggacaaaaag ccggaggatc agaaataaaa aataaataag ggacgacatt acactttgaa
    10801 tttttttaac ttatcagata tgaacagata ctttaccaca ggcagaaaca tcacgtggaa
    10861 gttatgtttt gcaattacac ttctgctagg cacgtttaat gctaaagcac agattccaac
    10921 agcatcattt gctgcagatt ctacagaagg atgtgtacca ttgacggttc actttagcaa
    10981 tacatccaca ttagctacaa gttatttctg ggattttgga aatggtaata catcaacatt
    11041 agccaatccg caaactgttt atttagctcc tggaaattat actgtaaagc tcattgccta
    11101 caacagtact tcaggacaat cagacacatt gataatacca aattatattg atgtagtttc
    11161 gtttccgaca gctaacttca catccaacgt tacatcagga tgtaaaccac tatctgtggt
    11221 atttaacaat acatcatcta actttacatc ttgtatttgg gattttggtg atggattttc
    11281 atctacacaa accaatccga cacatacata cagcaatgcc ggaacatata cagtaaagtt
    11341 agttgcatac tcttcttatg gttgttcaaa tgtaaaaaca cagacaaact atataaccgt
    11401 ttacggtcaa ccgacagcgc agttaacagc aaatccatct agcacatgtg atgccaatca
    11461 ggttattcaa ttcaattcat catctgccgg tgttgtgagt tatctatgga actttggtca
    11521 gccctcctcc ggtggtgcca attcttcaac acttcaggac cctacacaca cttatggaac
    11581 tcaaggttct tttaatgtga gtttgattgt tactgatgcc aatgggtgtt cagacactgc
    11641 tgttataaac aatatgatta caattggtac tgcactaact ccaacattta catggactaa
    11701 tggtgcagga tgtaagccgt taacaacaaa ttttactgcg ccttcaataa caggtgcaac
    11761 gagttatgca tgggactttg gtgacccttc atcaggagtt caaaacactt caacatctgc
    11821 aaaccctaca catacttatg caaactcagg aacatattct gtttcgctct atgttacaac
    11881 atcaagtgga tgcaatggag gtgtaacctt aaataatatc attgtagttg atcagccacc
    11941 ggtagctgca tttacatcta atccaacaac aggatgtaaa cctcttgcag ttcaatttaa
    12001 caatacttca acaggcgcgg ctacttacct ttgggaattt ggagatggta ctacttcaac
    12061 acttgcatca ccatcgaaga tatatacaac tgccggtact tataatgtaa cattacatgc
    12121 cttcagcact aatggctgtg aaacaattat tgtaaaaaac aactacattg ttgttaatga
    12181 aattaaatcc agttattatt ttcttccagg ctcaggctgt gcgccattaa gtgtcaattt
    12241 cataccaaca aataacagtg gttttgtttc ttatgaatgg aattttggtg atccggcttc
    12301 aggcgcactg aacacatcaa cattatctaa tccaacacat atttataata acattggaac
    12361 atactctact aaactgattg taacatcagc tgccggatgt aaagatacgc ttataaaaaa
    12421 ctttgtaaaa gtaagagcat catctgtagt atataccgtt cctgacacaa taaaaggttg
    12481 tcagccttat cctgtatcat ttaccaatcc attacccgga gctacagttt tccattggga
    12541 ttttggtgtt ccgtggttaa ctaatgacac tagtaacatt gcgaatccat catttacgtt
    12601 tgattccgta ggaattttta atgtaacatt aaatgcttat atgcctagca gtggtggtgc
    12661 aggttgctca caatttttca atcctgttgc catagtacag gtttttccaa tgctcatttc
    12721 tccaatcact tatattcagg ccacaccatg tgcaccatat ctgattcact ttagtgatac
    12781 cacaatggat gtcgtttcat gggattggga ttttggcgat ggcacaccac atgctacagt
    12841 tcaatttcct tcgcatacat acacacaacc gggtacatat acagttgtgc tgaatattgt
    12901 tctgcaaagt ggttgtatga ccagtttaag tacaacaatc actttaggtt atcctaatcc
    12961 aataacagta aataaaaaag ccggttgtca tgatgaacca tttacattcg gattaacagg
    13021 tggcccttgg acaaatgttc aatggaactt tggggatggc gttggcacat caacttctcc
    13081 aaatccaaca tatacttata atcaggtagg gactttcacg gtttctgtaa atgtaacaag
    13141 cagtgatgga tgtgttaata cttatactga tagtattact acaacaaacc cacaaccttc
    13201 ttttgttata aacggaccaa catcaagttg tacaaatcaa tttgtgttct ttacaaacac
    13261 atcaaccggt gcaacatctt attggtggga ttttggtgac ccaacatcat caacaggatt
    13321 ttcttcacta gtaaatccat caagaaattt tactacgcca aaagcatata ccatttcgct
    13381 aactgcaacc aatggtgcct gttcacgtac agttacttac ccggccttga ttagaattaa
    13441 cagagctgca ccgaatttct cattcacaca gagttctcaa tgcttcccaa ttactgttac
    13501 attaactgat cttacaggtc ctactcctgc tattcaatgg ggttggaatt tttataacgg
    13561 ttcaacagat tcaacgcaaa atccggttta cacattcaat gcgccaccaa catctgatac
    13621 aatttcatta tgggtcagag atacaaatgg atgctcagcg accaaacgac ttaaagggat
    13681 taagtacaaa caagctaagt ttactatgtc ggcaaccggt ggatgtaaac caagtacaat
    13741 ctgttttact gatgccagtg atacaacagt tgtttcaagg aaatgggatt ttggtaatgg
    13801 tgccacgtca actcaaacta atccttgtta tacctataca actgatggtg tatatactgt
    13861 aacgttgata acaactttct ccacaggttg caaagacaca ttagtcatgc cgaatgctgt
    13921 aaccatatca acaccagtag cagatttcag tgttcctaca caggcattct gtgcaccggc
    13981 actcgtgaac tttaacaatc tctccacaaa cgcaacaaca tactattggg attttggtga
    14041 tggctcaaat tctattgcta cgactccatc tcacgtgtac aacgttcctg gtgattatac
    14101 cattacttta attgccatca atggaacttg tcgcgataca atgaaaaaag taaattatct
    14161 gcatgtacca ggaactttct cttatttcaa cttagtaagt ctgcaaagtt gtgtaggaaa
    14221 cttagttcag tttacagaca gttcattgaa tgcacaaaca tggttatgga actttggtga
    14281 tggtgacagt tctacattac aaaatccaac acacttatat tctacaacag gtagctttac
    14341 agtttcattg attacaagtg ataaccttgg atgttcttca cattatatat tcagtaatcc
    14401 gatagttgtt catcctgtac caacagcggt ggccaataca atagatactg ttggttgtag
    14461 tccgctaaca tcaacattta cgcaaaactc aataaatgct acatcttatc tctggaactt
    14521 tggtgaccct gcttctggtg cgttaaacac agacactgca cctacttcaa cgcataccta
    14581 taataatgcc ggtaattatc aaccttactt tgttgcatat aataattttg gatgtagtga
    14641 tactatgtgg ttgcctaaga caattaatgt caatcagaca ccggtagcac agttcagttc
    14701 gtctgcaacc aatggttgta cacctttgac tgtaaacttt acaaatacat caactaaatt
    14761 aaatactcct caatatttgt ggaattttgg aaatggtaat acagccatta cttcaaatgc
    14821 cactgcagtt tatgccgatt cgggaaactt taatgttagt cttatagtta ctaatgctgc
    14881 aggatgtgcc gacacagcca ctgcacaaat tacaactgtt ctttcgccaa cggctattgc
    14941 ttcaacaaca gatactgttg gatgtagtcc acatactgtt aacttcacaa acatttctct
    15001 taatgcaaca agttatctgt ggaactttgg caatggtgtt acttcaacac ttgccaatcc
    15061 aagttatacc tacaataatg caggtaacta cactgtaaca ttaattgcaa ccaatgcatc
    15121 aggatgcagt gatacgcttg tttttccatc gcagattatt gttaaccaaa ctccgagagc
    15181 tcggttcaat atgagtcctg caacaggttg ttcaccactt acagtaaact tcacaaatac
    15241 ttctgcaaac acttccggag caacatacag ttggaacttt ggtaatggta atacagcatc
    15301 tactgcaaat acttcttctg tttatcctga ttcaggaaca tttgcagtta cacttttagt
    15361 gactaatggc aacggatgtt cagatgactc tgtaagaaat gtagttgtca atttatcgcc
    15421 tacggcaagt gcaactacaa acgatacaac aggatgtaat ccatacacaa gtacttttgt
    15481 aaacaactcg ataaacgcaa catcctataa ctggaatttt ggtgatggtg gaacatcaac
    15541 acaagtaaat ccaacacaca catatccggg aacaggtaac tttactgtta cattgattgc
    15601 aaccaataca gcaggatgta aagacacctt tgtttttaac aataccattc aggtaaacca
    15661 aacacctttg gcctcattca catcatctgc tactacagga tgtgcacctc tcaatgtcaa
    15721 cttcaccaat acatcaacag ttatttcagg agctacttac ttctgggatt ttggaaatgg
    15781 taacacttca actgttgcca acccatctac tgtatttgct gattcaggta cttatcaggt
    15841 tgtattgaat gtaaacaatg gaaatggttg tacccatact gcatcaaaat atataacggt
    15901 aaacattaca ccaactgcaa gttttgcaac cacagacact tcaggttgta cacctcacgc
    15961 tgtgagcttt acaaataact ctactaatgc tacaagctat cagtggaact ttggtaatgg
    16021 tgcaacttca acagcagtaa gtccaaacta tacttacact acaggtggat tgttcactgt
    16081 aacattaatc gccagcaatt cagccggatg tcgcgatacc tttgttttac catatcaaat
    16141 aaatgcaaag caatcgccca gagcaagatt taatatgagt ccgacttcgg gttgtacacc
    16201 actgaatgtg aactttacta atacaactgc gaacatgcaa agtcctactt tcaactggaa
    16261 ttttggaaat ggatcttcag caacaattgc aaatccttca acagtttatt cagactcggg
    16321 aacctatgcg gtaacattaa cagttaccaa tagcaatggc tgctttgatg attcgacccg
    16381 aattgttcgt gctaatcttt caccaacagc aatagctgca acaacagata ctgcaggttg
    16441 cgcaccttat acaagtacat ttacaaacag ctcacttaat gcaacatcat atcaatggaa
    16501 ttttggtgat ggtggtacgt caacggtagc aacgccaaca catacgtata gcacatcagg
    16561 taattat
//
LOCUS       DAOOOL010000047        16436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_289_length_16436_cov_85.354435, whole genome shotgun sequence.
ACCESSION   DAOOOL010000047 DAOOOL010000000
VERSION     DAOOOL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16436
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_289_length_16436_cov_85.354435"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PKU82_05565"
     CDS             <1..1337
                     /locus_tag="PKU82_05565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ82376.1"
                     /translation="NAIEMSITNDIIVTHYEGKPDLWNTKPPLLIWMQAGLIKLIGIN
                     ELAIRLPSAIAGLITCFALFFFLKRHTKNYWFGFIAALVLVTSQGYVGYHGTRTGDYD
                     ALLILFITLFSFSFYNYLSIGKIIHFKLFFLYLTLAILTKGIQPLIFLPGLLLIGFLN
                     HKDSGIKFQFKSTVFYSIFCIAVVAGYYFIRETQNPGYLKAVIENELGGRYLKTIESH
                     DHGFTFYYDNILNKQFNYWYLPMFIGVFIGLLNKNTTVKKLSIFCMILTLFYFLVISC
                     AQTKLEWYDLPMFPFMAILCSISIYELFLLVKHIPQFAERLKFNIAPYIILFFIFLFP
                     YRAIIDKVYFPKMYDYNNESIPFILKNLPSEVKTCVICWDNGYGPQINFYITKLSKQN
                     ITCYYKYYKDLSPGDVAIASQEYEKEYIEANYKTTLLLERAAAKMYKIENRN"
     gene            complement(1334..2827)
                     /locus_tag="PKU82_05570"
     CDS             complement(1334..2827)
                     /locus_tag="PKU82_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82377.1"
                     /translation="MKKLKYFIFVLLILISFYNLTSCSQKKEAISDLPGFVQIKNNQF
                     VLNGNPYFPLAVNYRVALQTNGKQLWPSAYKGYDDGNRYRFTNRDSSLEELNNDFQLI
                     ADMGFNSIRIVGIGEQYSVAKNSDELYIRADIGNEKDTMLLFNSQETYNQYFNALNDM
                     LQAANKAGLRVVLLSRLFHEVPATEKHWGRIMKHFQNDTTILAYDFFNEPLYFDSLER
                     NKTEPYYITKAWYELAKENAPHQLTTIGLTGIREVFEWDPNTLNVDFISYHPYEYEPE
                     QVRNEIYWYGNYVKKPWILGETSLPADNDSVPYSIQKNFAEATFNQTCNCGGIGYTWW
                     QYKDVDWFDFHSNFMGVVNRKGATLTSSGKFVIGTPKSMIDFIKHAKPLKDSTKCLKL
                     ENYYNYSNANVCRLIGKLVGPSGEPIAGGVIMAWNQYWSSSFHTVTKEDGTFELKGSF
                     PFYHWMASATMMTMIRGDVLPDTAKINVHEIPTLDMGILKVAKLKLD"
     gene            complement(2849..3475)
                     /locus_tag="PKU82_05575"
     CDS             complement(2849..3475)
                     /locus_tag="PKU82_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82378.1"
                     /translation="MKTFSGLITVLIFYSVQAFSQGSALMKEDAVQVNKFNDGVYFTL
                     TDLQHNTPTVTKDHLIKSYYDQSDFSIGQWATTQNLFYTAKDEHKLKVNRDSLWGYVE
                     NGTPYIYLNDRFHRFSTAGAISVFTESYPNIKAGLAPVVTEARSSSVINLFILSTGKI
                     VEFTPENVADAIEGNEQLYKEFNALKSWKQKRKKMYRFVERYNETEAF"
     gene            complement(3523..5274)
                     /locus_tag="PKU82_05580"
     CDS             complement(3523..5274)
                     /locus_tag="PKU82_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ82379.1"
                     /translation="MSTSGTAFNWKVLNRIMFYVKPFRFLFVATVVITVLLAVLAPLR
                     PWLTQQALDIDIAKHDIKGLTRTVAFMFGVLLLQSLLQFVYTTLTNRLGQEVILSLRN
                     NLFKKVSGFGLAYFDKTPLGITITRLVSDMETIADIFSDGLILIISDMLQVVVIIAVM
                     FYIHPQLAAISLSTIPVLLIATRVFQKNIRKTFNDVRIQVAKLNEFVQEHLVAMRIVQ
                     LFNREEEELNKFHEINAKHRDANIRSIWYYSLFFPVVEILSAVSIGLIVWYGGLNVLQ
                     SSISLGLLVAFIQYINQLFRPIRELADKFNTLQMGMVSSERVFKLLDEPFTEVNSGTK
                     SADNIIGKIEFRDVWFAYKNEEWILRGLSFIIPSGQSVAIVGSTGAGKTTIVSLINRL
                     YEIQKGQILIDGVDIRDYDLYSLRNAIGVVLQDVFLFSDSIKNNITLYREQDDIVIEA
                     LLKQTGAWHFVEKLPGQLHYNPGERGGLISTGQRQLLSFARVLAHQPKLIILDEATSS
                     VDSKLEQLLVNATEMLTKNRTSIIIAHRLSTIEKADRIIVLHKGAIAEDGNHQNLLER
                     KGIYAHLHDVQFKQPSA"
     gene            5380..6711
                     /gene="mtaB"
                     /locus_tag="PKU82_05585"
     CDS             5380..6711
                     /gene="mtaB"
                     /locus_tag="PKU82_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOZ82380.1"
                     /translation="MTRESTVAFHTLGCKLNFAETSTIGRQLQAAGYKKVSFSDTADV
                     YVINTCSVTENADRECRQHVNRALAANPEGCVIIIGCYAQLKPDAIATIPGVSLVLGA
                     SEKFNIPHYLQKLKKQTEAQVFSCDIADHHQFTSSYSEGDRTRVFLKVQDGCDYGCTF
                     CTIPLARGASRSNTIENVIENVKQIVDRGAKEIVLTGVNLGDFGKLPGEKKHATEFFQ
                     LVSALDALDADIRFRISSIEPNLLTEEIIELVSCSKKFMPHFHIPLQSGSDKILKLMR
                     RRYLTSHYTSRINKIKKLMPDACIGVDVITGFPGETEEDFLQTYKYLNEADISYLHVF
                     TYSERANTPAIEMKPVVPVAERKRRTNMLRILSEKKLGIFYRSQEGKTAKVLFEHENK
                     NGIMSGYSENYVRVSTPFNNEMVNKVIECKIGATDNDSHEVNCTILESITA"
     gene            6724..7878
                     /locus_tag="PKU82_05590"
     CDS             6724..7878
                     /locus_tag="PKU82_05590"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOZ82381.1"
                     /translation="MYPTLSDALHDLLGINIPLPIQTFGLILALSFFLAAYILILEFK
                     RKEQNGLLKSFTVTTIKGKPASTAELFWSGVVGFLVGFKLLDAILNYSDLVNDPQGFL
                     LSTRGNVIGGILLGAYSVWSKYKEKEKQKLAVPQTVTEVGYPHQFVMNITIAAAVAGL
                     IGAKIFHNLENIDDFLKDPVDALISFSGLTFFGGLICGALAVIWYSNKKLNVPPLVMC
                     DVAAPALMLTYGTGRLGCHFSGDGDWGIVNTLAKPSWFPFPDWMWSYNYPHNVIGEGV
                     PIAGCVGKHCYELIPPVFPTPLYEAIACILLFFVLWKLRTRIVTPGILFFVYLLFTGV
                     ERLAIEQIRVNTKYHIFGKAITQAELIAVACIIVSIVGITLLRRKNSGSS"
     gene            7942..8919
                     /locus_tag="PKU82_05595"
     CDS             7942..8919
                     /locus_tag="PKU82_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOZ82382.1"
                     /translation="MLHKSFFTAIVFLFLSTSLFSQKRIKIPVLDYGSVDEFVLKLKP
                     EQCVNIDTLAEVISREFDRPHFRFRAAFRWITENIAYDLKNQRHPQKANLNANDVFKN
                     KTALAEGYANILNAICAKMNITCLSVNGYLRRNQLPGTKIKKPNHIWSTVKLYDKWYL
                     MDVMMASGYFEKGDKIFTKRFTERYFALHPRQIILTHLPLNGNRQYLDTIVDKNTFLN
                     YPEVYDFFFDNGIYALRPADGVLPVYKGLKKRFYFKSMDSLAIGKIEIRDGKKSEKIQ
                     NTFTQVDSLVYFDYVFESNKKRTLDVFVDGLPLCTYFIVPDKKKKRSEY"
     gene            complement(8936..9811)
                     /gene="ygiD"
                     /locus_tag="PKU82_05600"
     CDS             complement(8936..9811)
                     /gene="ygiD"
                     /locus_tag="PKU82_05600"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HOZ82383.1"
                     /translation="MYRREFLSSIAGIGAIGTLSSFKNITDDLSNSDKMPVLFIGHGS
                     PMNGIENNEFSNKWKKLGQSLPKPKAIVVVSAHWLTKGTWITAMNHPKTIHDFGGFPQ
                     ALYNVQYKAPGSPELAQETKNIIKSVNADLDHDWGLDHGAWTVTRRMYPDANIPVLQI
                     SIDINKPASFHYQLGKELASLRTKGVLIIGSGNMVHNLGKIAWDKIDEVGFGYDWAVE
                     MHQLFKSKISDNDHQALINYASLSKSALMAVPTPDHYFPLMYVLGLQQKNENAEFFND
                     KLVAGSINMTSVKFG"
     gene            complement(9929..10774)
                     /gene="accD"
                     /locus_tag="PKU82_05605"
     CDS             complement(9929..10774)
                     /gene="accD"
                     /locus_tag="PKU82_05605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOZ82384.1"
                     /translation="MTWFKRIKEGITTSTREKKETPEGLWYKCPSCKAIVPSLEHEEN
                     KYVCPKCNYHDRIGSEEYFAVLFDDNQFTEFNTELTSADPLGFVDTKKYTDRLRDTIS
                     KTKLKDAMRTAVGKVNGNDLVVACMDFQFIGGSMGSVMGEKIAGAIDYSIQHKMPLMI
                     INKSGGARMMEAAFSLMQMAKTSAKLTRLADAGLPYISLLTDPTTGGVTASFAMLGDL
                     NIGEPGALIGFAGPRVVKETIGKDLPKGFQTSEFVLEHGFLDKIVDRRQLKDIIGQIL
                     SMFKN"
     gene            complement(10835..11890)
                     /locus_tag="PKU82_05610"
     CDS             complement(10835..11890)
                     /locus_tag="PKU82_05610"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOZ82385.1"
                     /translation="MLNKITELLGKDADKLLNHVCKTVPKETLHQPGNDFVDRIFIPS
                     NRSNQVIRSIQSLYNHGRLNGTGYMSILPVDQGIEHSAGASFAPNPMFFDPENIVKLA
                     IEGGCNAVASTYGVLASVSRKYAHKIPFIVKINHNEFLTYPNKFDQIMFGTVDEAWNM
                     GAVAVGATIYFGSEESSRQIVEVAQAFERAHELGMATILWCYLRNPGFKKDGIDYHTA
                     ADLTAQANHLGVTIQADIIKQKLPTNNGGYTAINFGKTHKDVYAKLSSEHPIDLCRYQ
                     VLNCYNGRMGLINSGGESKGATDMAEAVTTAVINKRAGGQGLISGRKAFQKPFKDGVE
                     MLNAIQDVYLSKDVTLA"
     gene            12002..12997
                     /locus_tag="PKU82_05615"
     CDS             12002..12997
                     /locus_tag="PKU82_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOZ82386.1"
                     /translation="MKIVIPVAGIGTKLRPHTHTQPKTLVPVAGHPILAHIIDYFVQS
                     GYDEFVFIIGYMGEKIEEFVKSKYPSLKAAFVLQEPREGTGHSVWFARNHVHAEDEML
                     IVLGDTIIDFDINVFKQAEQSLLGVKKVDDPRNFGVVELNDEGFVSKLHEKPVIPKSN
                     LALVGIYFIKQAGHLFEALNYIIENNLKTRDEYHLTDAMMRMIEEGEKIKVFHVGNWF
                     DCGNKESLLETNAILLDRIAETNPKPQKFPNTIIIPPVSIAYNCDIADSIIGPHVSIG
                     EHTIVKYSIIKDSIIGSYSQLESAVLHRSVIGSDAFLKGLSQSLNIGDSTEIDFS"
     gene            13116..14072
                     /locus_tag="PKU82_05620"
     CDS             13116..14072
                     /locus_tag="PKU82_05620"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82387.1"
                     /translation="MKKLFTLITAVALGSLTSNAQIYDNGTIVNNPVLGVGGADVSSL
                     HDGLNSLGMNHASGLGYRVADDFIIPAGQTWTIDSIAFLAYQTNSGNASTITDVNCAI
                     FNGSPAIGGSIVLGDTITNLMQSTYFSNIYRTADAAFTNTARPVMRNIVVPQSAWSLP
                     AGTYWVAWQTGGTLTSGPWAPPLTNANMATGDAIQFNPDGSIWNPILDGGATADPQGL
                     PFQIYGTVTTSINSIEVEDALTILPNPSKGNFMINATFSNRCNLEINVMDLQGRSIYS
                     ENVSDVSVLTKNVVLNDVSKGIYLLKLTSDSGKVLTKKIVVD"
     gene            14224..15183
                     /locus_tag="PKU82_05625"
     CDS             14224..15183
                     /locus_tag="PKU82_05625"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82388.1"
                     /translation="MKKLFTLLFVILICYIQGQAQIYSNGTIINHPGVGQGGADISCF
                     HHGLASLGFGQQLLEGYRIADDFNIPAGQIWTIDSIRFYAYQSNSGIVSTINAVNCEI
                     FNGSPASGGTIVLGDTITNLLQNTRFSNIYRTVDGQFNSVSRPVMYSVIVPQSAWTLT
                     SGNYWLSWQSGGNPSLSGPWVPPLTDTLNTTTGDGLLYIPDSSMWLPALDVALSTPQG
                     FPFEIYGTITTSINTIEVEDALTILPNPSKGNFMINASFANRCNLEINIMDLQGRSIY
                     SENVSDVSVLTKNVVLNDVSKGIYLLKITTDTGKTSIKKITIN"
     gene            complement(15258..15806)
                     /locus_tag="PKU82_05630"
     CDS             complement(15258..15806)
                     /locus_tag="PKU82_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82389.1"
                     /translation="MNFKSGLFYLSIILLFTACQKDYNDPGTGPSQYYGTRSGVSIRA
                     ILLAQMPATDPSGNSWDVADSVDTAGRADIYYRFKDIDSIDYPEFVQNFHFDNVNPDS
                     LPLPYFLTTPYPLRAIGSNVQFDIYDFEKSLSSPAIDSTLMKSFNFSVVAGDSLIENP
                     YPDSLVLAEDGYVVLLYLNWKK"
     gene            complement(15893..16054)
                     /gene="rpmH"
                     /locus_tag="PKU82_05635"
     CDS             complement(15893..16054)
                     /gene="rpmH"
                     /locus_tag="PKU82_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOZ82390.1"
                     /translation="MVRTYQPSKRKRKNKHGFRERMATANGRRVLASRRAKGRKKLTV
                     SSESKVRGK"
     gene            complement(16121..>16436)
                     /locus_tag="PKU82_05640"
     CDS             complement(16121..>16436)
                     /locus_tag="PKU82_05640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ82391.1"
                     /translation="KEMANQLSLSNVTVFNKRAEDVSEKVDFVVSRATAPLSDLVKWS
                     RGRINSKGKASIPNGLICLKGGDLTEELKPFENKLFTENVTAFIQEEFYKEKKIIYLP
                     LV"
BASE COUNT         5219 a         2888 c         2864 g         5465 t
ORIGIN      
        1 ctaatgctat tgaaatgagt attaccaatg atattatagt tactcattat gaaggaaaac
       61 ctgacttatg gaatacaaaa ccgcctcttt taatttggat gcaggccgga ttaataaaac
      121 tcataggaat aaatgagttg gcaataaggc ttccatctgc aatagcgggc ttaattactt
      181 gttttgcatt atttttcttt ttaaaacgcc acactaaaaa ttactggttt gggtttattg
      241 ctgcccttgt attggttaca tctcaagggt atgtgggata tcatggaaca cgaacaggag
      301 attatgatgc cttattaatt cttttcatca cactttttag ttttagtttt tacaattatt
      361 tatccatagg aaaaataatt cattttaagt tgtttttttt gtatttaaca cttgccatat
      421 taacaaaagg aattcaacca ttaatatttt tgccaggatt actcctaata ggattcctca
      481 atcataaaga ttccggaatt aaatttcaat ttaaatcaac tgtgttttac agcatatttt
      541 gtatagcggt tgttgctggt tactatttta ttagggagac acaaaatccg ggatatttga
      601 aagctgtaat tgaaaatgag cttggcggaa gatatttaaa aacaattgaa agccatgatc
      661 atggatttac attttattat gataatattc ttaataaaca attcaactat tggtacttac
      721 caatgtttat tggtgttttt ataggattat taaacaaaaa tacgacagtt aagaagttat
      781 caatattttg catgatatta accctatttt attttttggt aatctcatgt gctcaaacaa
      841 aattagaatg gtacgatttg cccatgtttc cttttatggc aattttatgt agtattagta
      901 tttacgaatt atttttatta gttaaacaca tcccgcaatt tgcggaacgg ttaaaattta
      961 atattgcacc atatataatt ctgtttttta tttttttatt cccttatcgt gctattattg
     1021 ataaagtcta tttcccaaaa atgtatgatt acaataatga aagcattcca tttattctta
     1081 aaaatctacc ctcggaggtc aagacttgtg taatttgctg ggataatgga tatggacctc
     1141 aaattaattt ctatataaca aaactatcaa agcagaacat tacttgttat tacaaatatt
     1201 ataaagattt gtctcctggt gatgtagcaa ttgcatcgca ggaatatgaa aaagagtata
     1261 tagaagcaaa ctacaaaaca actttgttat tagaaagagc tgcagcaaaa atgtataaaa
     1321 ttgaaaaccg taattaatcc aatttcaatt ttgcgacttt caaaataccc atatccaatg
     1381 ttggaatctc atgaacattg atttttgctg tatccggtaa aacatcacct cgtatcatgg
     1441 tcatcatggt agcagatgcc atccaatgat aaaagggaaa acttcccttc aattcaaaag
     1501 tcccatcttc ttttgttact gtatggaaag aacttgacca atattgattc catgccatta
     1561 taacaccacc cgctataggc tctccagatg gtccaactaa tttcccaatt aagcgacaaa
     1621 catttgcatt ggagtaatta tagtagtttt ccaattttaa acatttagtt gagtctttta
     1681 aaggttttgc atgttttata aagtctatca tagatttggg tgtacctata acaaacttgc
     1741 ctgatgaagt caatgtagct ccttttctgt tgacaacacc cataaaattg gaatgaaaat
     1801 caaaccaatc aacatcttta tattgccacc atgtatagcc tatgccacca caattgcaag
     1861 tttgattaaa agtagcttcc gcaaaatttt tctgaatact atatggaaca gaatcattat
     1921 cggccggcaa tgaagtttct ccaagaatcc aaggtttctt aacataattt ccataccagt
     1981 aaatttcatt acgaacttgt tcaggttcat attcataagg atgatatgaa atgaaatcta
     2041 catttaatgt attaggatcc cattcaaata cttcacgaat gccggtcaat ccaattgtag
     2101 ttaactgatg aggtgcattt tccttagcca gttcatacca ggctttagta atatagtatg
     2161 gctccgtttt atttctctcc aatgaatcga aatacaaagg ctcattaaaa aaatcatacg
     2221 ccagaattgt agtatcattc tgaaaatgtt tcattattct tccccaatgt ttttctgttg
     2281 ctggtacttc atgaaaaagt cttgataaca aaactactct taggcctgct ttatttgctg
     2341 cctgaagcat gtcgtttaat gcattgaaat attggttgta agtttcctgt gaattaaaaa
     2401 gtagcatcgt gtccttttca ttacctatgt cagctctaat gtataattcg tctgagttct
     2461 ttgcaacaga atattgttca cctattccaa caattctgat cgaattaaat cccatatcag
     2521 ctataagctg aaagtcatta ttcaattctt ccagacttga atctctgttt gtgaaccggt
     2581 agcgattacc atcatcatat cctttatatg ctgatggcca aagttgtttt ccattagttt
     2641 gaagtgctac tctataattg actgccaatg ggaaatatgg atttccatta agaacaaatt
     2701 gattgttttt aatttgtaca aaaccgggta aatcagatat cgcctccttt ttctgtgaac
     2761 aacttgttaa gttgtaaaag gaaattaaaa tgagtaatac aaaaataaaa tacttcaatt
     2821 ttttcataaa ataaatttta attaaggttt aaaaggcttc tgtttcatta taccgctcta
     2881 caaacctata catttttttg cgtttctgtt tccacgattt taatgcatta aactccttat
     2941 aaagttgttc attgccttca attgcatcag caacattttc aggtgtaaat tccactattt
     3001 ttcctgtgga aagtataaac agattgataa ctgatgaaga tcttgcttct gttactacag
     3061 gtgccagacc ggctttgata ttaggataac tttcagtaaa tactgaaatg gctcctgccg
     3121 ttgaaaaccg atgaaacctg tcgttaagat aaatgtatgg cgttccattc tctacataac
     3181 cccataagct atctcggttg actttcaatt tatgttcatc ttttgcagta taaaataagt
     3241 tttgtgtggt tgcccactgg ccgatactga aatcagattg gtcgtaatat gattttatga
     3301 gatgatcttt agtaacagtt ggtgtgttat gttgcaggtc tgttaatgta aaataaacac
     3361 catcattaaa tttattaacc tgtacggcat cttctttcat caacgcagaa ccttgagaaa
     3421 aagcttgaac agaataaaaa attaataccg tgataagtcc ggaaaatgtt ttcattttat
     3481 gaaatagtat tctgcaaaga aacaaaaatc aactcttata agttatgctg atggctgttt
     3541 aaattgaaca tcatgaaggt gagcataaat acctttcctt tctaatagat tttgatgatt
     3601 tccgtcttcg gcaattgctc ccttatgtaa aacaataatg cgatcagctt tttctatagt
     3661 tgataaacga tgggcaataa taattgaagt ccggtttttt gtcagcattt ctgttgcatt
     3721 aaccaaaagc tgctccagct tactgtcaac actgcttgtt gcctcatcca aaataattag
     3781 tttaggttga tgtgccaaca ctctggcaaa tgacaatagc tgcctctgtc cggtgctgat
     3841 taaccctccc ctttctccgg gattgtaatg tagctgaccg ggtaactttt ctacaaaatg
     3901 ccaggctcct gtttgtttca acaaggcttc aataacaata tcatcttgtt ctctatagag
     3961 tgtaatattg ttctttatac tgtcagaaaa aagaaagaca tcctgaagga caacgcctat
     4021 agcatttctc aggctgtata aatcataatc tctgatatca acaccgtcaa ttaaaatctg
     4081 tcctttttga atttcatata agcggttaat caaacttaca atagtagttt tccctgcgcc
     4141 tgttgatcca acaatcgcta cagattgtcc tgaaggaatg ataaaggata agccacgcaa
     4201 aatccactcc tcatttttat aggcaaacca cacatctctg aattcaattt tgcctattat
     4261 attatcggca ctttttgtgc ctgaattaac ttctgtaaat ggttcatcga gcaacttgaa
     4321 aactctttct gaacttacca tgcccatttg taaggtgtta aacttatcag caagttcacg
     4381 aattggcctg aacaattggt tgatatattg aataaaagca acaagtaagc ctaaactgat
     4441 agaactttga agtacattta aaccaccata ccaaactata agccctattg aaacagcaga
     4501 gagaatttct acaactggga aaaacaaact gtaataccat atagaacgga tattggcatc
     4561 gcgatgcttg gcattaattt catgaaactt atttaactcc tcttcttcac gattaaaaag
     4621 ttgtacaatt ctcattgcaa ctaaatgctc ctgaacaaat tcgtttagtt tagctacctg
     4681 tatgcgcaca tcattaaaag tttttctgat atttttctga aaaactctgg tagcaatcaa
     4741 cagaacagga atagtagata aagaaattgc agcaagttgt ggatggatgt aaaacattac
     4801 agcaataatt accactacct gaagcatatc acttattata agaatcaacc catcagaaaa
     4861 tatatctgca atggtttcca tatcgcttac caatcgtgta attgtaattc caagaggagt
     4921 tttatcaaaa taagctagcc caaaaccgct gaccttctta aacaaattat tacgtaatga
     4981 aagtattact tcctgtccaa gtcggtttgt taaggtagta tagacaaact gaagcaatga
     5041 ttgtagtagc agaacaccaa acatgaaagc aacagtgcgt gtaagacctt tgatgtcgtg
     5101 ttttgcaatg tcaatatcaa gtgcctgctg tgtaagccag ggtcgtaatg gtgccaatac
     5161 agcaagcaac acagtgataa ccactgtggc tacaaaaaga aaccggaaag gctttacata
     5221 gaacataatc ctgtttagca ccttccaatt aaatgctgtt ccgcttgttg acattatttt
     5281 tgaatagact gcaaaattca cactcttatt gattagttca aaatgaaagg agttttttgg
     5341 aatgtaaagt tcagcggtaa ttgctatttt tgcacgctaa tgacaaggga atctactgta
     5401 gcttttcaca ccttaggttg taaattgaac tttgctgaga cttcaacaat tggccgtcaa
     5461 ttgcaagctg caggctacaa aaaagtttcg ttttctgata ctgctgatgt ttatgtcata
     5521 aatacctgtt ctgttactga aaatgctgat agagaatgcc gccaacacgt caacagagct
     5581 ttagcagcaa atcccgaagg atgtgtcatc attattggct gttatgcaca gctaaaacca
     5641 gatgcaattg ctacaattcc gggtgttagc cttgtattag gtgcaagtga aaagttcaac
     5701 attccgcatt atttgcaaaa gcttaaaaag caaaccgaag cacaggtttt ttcttgcgac
     5761 attgccgatc atcatcagtt tacaagcagc tactccgaag gtgacagaac tcgtgttttt
     5821 ctgaaagttc aggatggctg tgattatggt tgcacatttt gcaccatacc attggcgcgt
     5881 ggcgcaagca gaagtaatac aattgaaaat gtcatagaaa atgtaaaaca aattgttgat
     5941 agaggtgcaa aagagattgt tctgacagga gttaatttag gtgactttgg taaactgcct
     6001 ggtgagaaaa agcatgcaac agaatttttt caattagttt ctgcattaga tgctttggat
     6061 gctgatatcc gattcagaat ttcgtctatt gaaccaaacc tgctaacaga agaaatcatt
     6121 gaattggtta gctgctctaa gaaatttatg ccacattttc atattccatt acaaagcggt
     6181 tctgataaaa ttctgaaact gatgcgcaga cgttacctga cttcgcatta cacatcacgg
     6241 ataaataaaa ttaaaaagtt gatgcccgat gcctgtattg gtgttgatgt gattacagga
     6301 tttcccggtg aaacggagga agattttctt cagacttata aatatttgaa cgaagctgat
     6361 atttcttacc tgcatgtttt tacttattct gaaagagcaa acactcctgc tattgaaatg
     6421 aagcctgttg ttccggttgc agaaaggaaa agaagaacca atatgctgcg catactttcg
     6481 gagaaaaaac tcggaatatt ttatcggtca caagaaggta aaacagccaa agtacttttt
     6541 gaacacgaaa acaagaatgg aataatgtca ggctattccg aaaactatgt gagagtttca
     6601 acacctttca ataacgaaat ggtaaataaa gtaattgaat gtaaaatcgg tgcaaccgac
     6661 aatgacagcc atgaagttaa ctgtacaatt ttagaatcaa tcacggctta ataatttctg
     6721 cccatgtacc ctactctatc cgatgcactc catgatttat tgggcataaa tattccattg
     6781 ccaattcaaa cattcgggct catattggca ttgtcatttt ttttggctgc atatatttta
     6841 atcttagagt ttaaacgcaa agagcaaaat ggtttattaa agtctttcac cgttactacc
     6901 attaaaggaa aacctgcatc aacagcagag ttgttttggt caggtgttgt aggattttta
     6961 gttggcttta aattgcttga tgccattttg aattacagtg accttgttaa tgatccacaa
     7021 ggctttttat tatcaacaag aggtaatgtc attggcggga tattactcgg tgcctattct
     7081 gtttggagca aatacaaaga aaaagaaaag caaaaattgg cagtgccgca gactgttaca
     7141 gaagttgggt atcctcatca gtttgtaatg aatattacca ttgctgctgc cgttgcagga
     7201 ttaataggtg ccaaaatatt tcataatctt gaaaacattg atgactttct taaagatcct
     7261 gttgatgcat tgatatcgtt cagcggttta actttttttg gcggattgat ttgtggtgct
     7321 ttagctgtga tatggtatag caataaaaaa ttaaatgtgc cgccattggt gatgtgtgat
     7381 gttgctgcac ctgcattaat gctgacttat ggcacaggca gattaggttg tcacttttca
     7441 ggcgatggtg actggggaat tgtaaataca cttgcaaaac cttcttggtt tccatttcct
     7501 gattggatgt ggagttataa ctatccacac aatgtaattg gagagggtgt tcctattgct
     7561 ggatgtgttg gcaaacattg ctatgaattg atacctcctg tttttcctac accattatat
     7621 gaagcaattg cctgtatttt attattcttt gttttgtgga agctacgaac tagaatagtt
     7681 actcccggaa tacttttctt tgtttacctg ctattcacag gtgtagaacg tttggcaatt
     7741 gaacagatca gagtcaatac aaagtaccac atatttggaa aagcaattac acaggcagag
     7801 cttatagctg ttgcatgtat tattgtttca atcgttggaa ttacgctttt aagaagaaaa
     7861 aattccggtt catcttaaag cctgttgact ttaaaagaat attgtggtaa tttggacaaa
     7921 attttttgac caaaaccata tatgctccat aaaagttttt ttacggcaat agtttttttg
     7981 tttctctcca catcgctttt ttcacaaaag aggattaaaa ttcctgtact tgattatggt
     8041 tctgttgatg agtttgtgtt aaagttaaaa cccgaacaat gcgttaacat tgatacatta
     8101 gctgaagtaa tcagcagaga atttgaccgc cctcattttc gtttcagggc tgcattcaga
     8161 tggattacag aaaacatagc ttatgattta aagaaccaac gccatccgca gaaagcgaat
     8221 ctcaacgcta acgatgtttt taagaacaaa actgcactag ccgaaggcta tgcaaatatt
     8281 ctgaatgcta tctgtgctaa aatgaatatc acatgccttt cggtgaatgg ctatttaaga
     8341 agaaatcaat taccgggaac aaagattaaa aaaccaaatc atatctggag tactgttaaa
     8401 ctatatgata aatggtactt gatggatgtg atgatggcaa gtggctattt tgaaaaaggt
     8461 gataagattt ttacaaaacg ctttactgag agatattttg cattacatcc gcgtcagata
     8521 attttaacgc atcttccatt aaatggaaat cgtcaatatt tagatactat tgttgacaag
     8581 aatacttttt tgaactatcc tgaagtttat gatttctttt ttgataacgg catttatgca
     8641 ttaagacctg ctgatggtgt attacctgtt tacaaaggtt tgaagaaacg tttttatttt
     8701 aaatctatgg atagccttgc tattggtaag attgaaattc gtgatggtaa aaaatctgaa
     8761 aagattcaaa atacttttac acaggttgat agtcttgttt atttcgatta tgtatttgaa
     8821 tcgaataaaa aaagaacatt ggacgtgttt gttgatggac taccattatg tacttatttt
     8881 atagttcccg ataaaaagaa aaaacggtca gaatattaac tgttggaatc tgcgtttacc
     8941 cgaattttac agaagtcata ttgattgaac ctgctaccaa cttatcgtta aaaaattctg
     9001 cattttcatt tttttgttgc agcccaagta catacattaa cggaaaataa tgatccggtg
     9061 ttggcacagc cattaaagct gacttgctaa gagatgcata gttaatcagt gcctgatgat
     9121 cgttgtctga aattttagat ttaaaaagtt gatgcatttc aacagcccaa tcataaccaa
     9181 agccaacttc atcaatttta tcccatgcaa tttttccgag gttgtgaacc atattaccac
     9241 ttccaattat caatactcct tttgtgcgaa gtgaagcaag ttcttttccc aattgataat
     9301 gaaatgatgc cggtttatta atgtcaatac tgatttgaag cacaggaata ttcgcatcag
     9361 gatacattcg tcttgtaaca gtccatgctc catggtcgag cccccagtca tgatctaaat
     9421 cagcatttac tgacttgata atattttttg tttcttgtgc caattcagga ctgcccggtg
     9481 ctttgtattg tacattataa agtgcttgcg gaaatccacc aaaatcatga atagttttag
     9541 ggtggttcat tgcagtaatc caagttcctt ttgtcagcca atgtgcagaa acaacaacaa
     9601 ttgcttttgg tttaggtaat gattgaccta attttttcca tttattgctg aactcattgt
     9661 tttcaatgcc attcattgga gagccatgtc cgataaacaa tacaggcatc ttatcggaat
     9721 tactcagatc atcagtaatg tttttaaaag aacttaaagt tccaatagct cctatacctg
     9781 caattgatga taaaaattct ctgcgataca tttgattttt aataattatc aataacgttt
     9841 acagcataaa cattgtttac ttgtaacaaa ctaaaacatg taaaggatta gttgaattat
     9901 ttattcagaa aaagtaaact gaaagcaatt agtttttaaa catgcttagt atctgaccaa
     9961 tgatatcttt taattgtctg cggtcaacaa ttttatctaa aaaaccatgt tcaagaacaa
    10021 attctgaagt ctgaaatcct ttgggcaaat ctttacctat tgtttctttt acaactcgtg
    10081 gaccggcaaa tccaattaat gctccgggct ctccaatgtt taaatcacca agcatggcaa
    10141 aagatgctgt cacacctcct gtggtaggat cggtaagtaa actaatataa ggcaagcctg
    10201 catcggcaag tctggtaagc tttgctgatg tttttgccat ctgcatcaaa gaaaatgctg
    10261 cttccatcat acgggcacca cctgatttgt ttataatcat caatggcatc ttatgctgaa
    10321 tactatagtc aattgctcct gcaatttttt cgcccattac agagcccata cttcctccaa
    10381 taaactgaaa gtccatgcaa gcaacaacta aatcgttacc gttaacttta cctacagcag
    10441 tacgcatagc atctttcaac ttcgttttag aaatggtatc acgtaagcgg tcagtatatt
    10501 tttttgtatc aacaaaacct aaaggatcgg cagaggttag ttctgtatta aactctgtaa
    10561 actgattgtc atcaaaaaga actgcaaaat attcttcaga accaattctg tcgtgataat
    10621 tgcactttgg acatacatat ttattttcct catgctccaa tgatggaaca atagctttac
    10681 atgatgggca tttataccac aaaccttcgg gagtttcttt tttctctctt gtagaggtgg
    10741 tgatgccctc tttaattcgt ttaaaccaag tcatttataa tcagtttgcg tttttaaatg
    10801 ttgggtttat caactgatat actgtttata ttacttatgc taaagtaaca tcttttgaaa
    10861 gatatacatc ctgtattgca tttaacattt caacaccatc tttaaatggt ttttggaaag
    10921 ctttacgtcc tgaaattaat ccctgaccac cggctctttt attgataact gctgtggtta
    10981 ctgcttcggc catatctgta gcacctttag attctccacc tgaattgatt aatcccatac
    11041 ggccgttata acaattcaat acctgatagc gacaaaggtc aataggatgt tcagaagaaa
    11101 gctttgcata aacatcctta tgagtttttc cgaagttaat agcagtgtat ccaccattat
    11161 tagtaggtag tttttgttta ataatatctg cctgaatagt tactcctaaa tgatttgcct
    11221 gagcagttaa gtctgcagca gtatggtaat ctattccgtc ttttttaaat cccgggttgc
    11281 gtagataaca ccagagaatt gttgccatac ccagttcgtg agctcgttca aatgcttgtg
    11341 ccacttctac aatttgtctt gatgattctt cgctgccaaa ataaattgtt gcaccaacag
    11401 ccacagcgcc catgttccaa gcctcatcaa cagtaccgaa cataatctga tcaaatttat
    11461 ttgggtatgt cagaaactca ttatggttaa ttttaacaat aaaaggaatc ttatgagcat
    11521 actttctgga aacagatgca agtactccat aagttgaagc aactgcatta cagccgcctt
    11581 ctatggctaa ttttacaatg ttttcagggt caaaaaacat tgggtttggg gcaaagcttg
    11641 ctccggcaga gtgctctatt ccctgatcta ccggcagaat ggacatatat cctgtaccat
    11701 tcaaacgacc atgattatag agtgattgta tgctacggat tacctgatta cttctgttcg
    11761 aagggataaa aatgcggtca acaaagtcgt tacccggctg atgtaatgtt tcctttggaa
    11821 cagttttaca aacgtgattt aataatttat cggcatcctt gccaagtaat tcggtaattt
    11881 tgttcaacat atcgtgcgtt taaatatcag ttaagtgtgg caaaactaca ttttttttta
    11941 ttccatgcca tcagacttat gctgttctgt agtttaatat ttttgcttaa aataatttga
    12001 aatgaaaatt gtaatccctg tggccggtat cggaacaaaa cttcgtccac atacacatac
    12061 acaaccgaag acattagtgc ctgttgcagg ccatcccatt cttgcccaca tcattgatta
    12121 ttttgtgcaa tccggatatg atgagtttgt atttattatt ggttacatgg gtgaaaaaat
    12181 tgaagaattt gtaaaaagca aatatccttc acttaaagca gcatttgtat tgcaggaacc
    12241 acgcgaaggt accggtcatt ctgtttggtt tgctcgaaac catgttcatg cagaagatga
    12301 aatgctcatt gtattgggcg atacgataat tgattttgac attaatgttt ttaagcaagc
    12361 cgaacagtct cttttaggag ttaaaaaagt agatgatcca agaaattttg gtgttgttga
    12421 attgaatgat gagggtttcg tttccaagtt gcatgaaaaa ccggtaatcc caaaatcaaa
    12481 tcttgcactt gtcggtatct attttattaa acaagcaggg catttgtttg aagcattgaa
    12541 ttacattatc gaaaacaacc tgaaaacaag agatgaatat catctgactg atgccatgat
    12601 gcgaatgatt gaagagggtg agaaaattaa agtctttcat gtgggcaatt ggtttgattg
    12661 tggaaacaag gaaagtctcc ttgaaaccaa tgcaatttta ttagacagaa ttgcagaaac
    12721 aaatcctaaa ccacagaagt ttccaaatac aatcataatt cctccggtga gtattgcata
    12781 caactgcgat attgctgatt ccattattgg tcctcatgtt tctattggcg agcataccat
    12841 cgtaaaatat tctatcatta aagattcaat tattggctcc tattcgcagt tagaatctgc
    12901 agttttacat cgttctgtaa ttggaagcga tgcatttcta aaaggactaa gtcagagctt
    12961 gaatattggt gatagtacgg agatagattt tagttgatgt tataaacata ttcattggaa
    13021 gtaatgttga tattttaaga aatctaatgt aaaatatagt gattgtaata tttaaacctt
    13081 tacttttgat agataatttc aaaatcaaaa aaaatatgaa aaaactattt actcttatta
    13141 cagcagttgc cttaggaagt ttaacttcta atgcacaaat ctatgacaat ggtacaattg
    13201 taaataatcc ggtattaggt gtcggtggag cagatgtatc ctccttacat gatggactga
    13261 attcattagg tatgaatcat gcttccggac tgggttatcg tgttgctgat gatttcataa
    13321 ttcctgcagg tcagacatgg acaattgatt ctattgcatt tcttgcttat caaaccaatt
    13381 ctggaaacgc atctacaatt acagatgtaa actgtgcaat ttttaatggt tcaccggcaa
    13441 ttggtggttc tattgttttg ggcgatacaa taactaattt gatgcaaagc acttatttct
    13501 caaatatcta tcgtacagcg gatgcagcat ttacaaatac agcgcgtccg gtaatgcgaa
    13561 acatagttgt gccacaaagt gcatggtcac taccggctgg aacttattgg gttgcctggc
    13621 aaacaggcgg tacattaaca tctggtccct gggcacctcc tttgactaat gctaatatgg
    13681 caaccggaga tgcaattcag ttcaatcctg atggttcaat ttggaatcca attctggatg
    13741 gtggcgctac agctgaccca caaggcttac catttcagat atatggaact gtaacaactt
    13801 ccatcaattc aatagaagta gaagacgcac ttacaattct accaaatcca tcaaagggaa
    13861 attttatgat taatgctact ttttcaaacc gttgtaatct ggaaattaat gtaatggatt
    13921 tgcaaggcag aagcatctac tcagaaaatg ttagtgatgt ttctgtttta actaaaaatg
    13981 ttgttttgaa tgacgtttcc aaagggattt atctgttgaa acttacaagt gattcaggta
    14041 aagttcttac caagaaaatc gtagttgact aataatttta taaacttttt aaaggctgta
    14101 atttttagaa attgcagcct ttttttttaa aaaattgtag aacaaactcg attagcctat
    14161 gaatatttct atagttgaat tgtattatat gggtattgaa ttaagtttgt aaaaaattta
    14221 gatatgaaaa aattatttac tttattattt gtaatactta tttgctatat tcagggtcag
    14281 gcacagattt attccaatgg cactattatt aatcacccgg gtgtaggtca aggtggtgca
    14341 gatatatcct gttttcatca tggattagct tcattaggat tcgggcaaca attgcttgaa
    14401 ggctatagaa tagcagatga ttttaatatc ccggcaggtc aaatttggac aattgactct
    14461 attaggtttt atgcatatca gagtaattca ggaattgtat ctacaataaa tgctgtaaat
    14521 tgtgaaatct ttaatggctc tccggcatca ggcggtacaa ttgttttagg tgatacaata
    14581 actaatctgt tacaaaacac tcgcttttca aatatttaca ggactgtaga tggtcagttt
    14641 aattccgtgt cacgtccggt aatgtacagt gtaattgtac cacaaagtgc atggacactt
    14701 acttcaggta attattggtt aagttggcag agtggtggaa atccatctct ctcaggacca
    14761 tgggtaccgc ctttaactga tactttaaat actactacag gagacggatt attatatatt
    14821 cctgatagct ctatgtggct tcctgcatta gatgttgctc tatcaacacc tcaaggattt
    14881 ccttttgaaa tatatggaac tattacaact tcaattaata caatagaagt agaagacgca
    14941 cttactattc taccaaatcc atcaaaagga aattttatga ttaatgcttc ttttgcaaac
    15001 cgctgtaatc tggaaattaa tataatggat ttgcaaggca gaagcatcta ctctgaaaat
    15061 gttagtgatg tttctgtttt aactaaaaat gttgttttga atgacgtttc caaagggatt
    15121 tatctgttga aaattactac agatacagga aaaacaagta taaagaagat tacaataaat
    15181 taattgatta aagtactttt agaaaaagct gcacccttat agagtgcagc ttttcatttt
    15241 tataaataat cacaacatta ctttttccag ttcaaataaa gcagaactac ataaccatct
    15301 tctgccagta ccaaactatc aggataagga ttctcaatta gtgaatcgcc tgcaacaact
    15361 gaaaagttaa atgatttcat taaggttgag tcaatagctg gcgatgaaag gctcttctca
    15421 aaatcataaa tatcaaactg aacattgctg ccaatggcac gtaatggata aggcgtagtt
    15481 aaaaaatatg gcagcggcaa actatcagga tttacattgt caaaatgaaa attctgaaca
    15541 aattcaggat agtcaataga gtctatgtct ttaaaacggt aataaatatc tgcacgacct
    15601 gctgtgtcaa cagagtctgc tacatcccaa ctatttcctg atggatctgt agcaggcatt
    15661 tgcgccaata aaatagccct gatagaaaca ccgctgcgtg ttccataata ttgcgatggg
    15721 ccggtgccgg ggtcattata atctttctga caagcagtaa acaagagaat aatagacaaa
    15781 taaaagagac ctgatttgaa attcatgatt acaagaaact ttaataattg gtgctaaaat
    15841 aaaaaagcca ccttaaagaa ggcagctttt tgatatttta taatgtagtt atttatttac
    15901 cacgaacttt actttcactt gaaacagtca gttttttacg accttttgct ctgcgtgaag
    15961 ctaacactct gcgaccatta gcagttgcca ttctttcacg aaaaccatgt ttgttttttc
    16021 tcttacgctt tgagggttga tatgtcctta ccataatact tcagttttta ttaatgctgc
    16081 aaaattcttt ttagaggcgg caaagatata attaattctt ttatactaaa ggcaagtaga
    16141 ttattttttt ttctttataa aattcttcct gtataaatgc cgtaacattt tctgtaaata
    16201 gcttattttc aaatggtttt agctcttcgg ttaaatctcc cccctttaaa caaataagtc
    16261 catttgggat tgatgccttg cctttagaat taattctacc cctactccat tttaccaaat
    16321 ctgagagtgg tgccgtagct ctggaaacaa caaagtcaac tttttctgat acatcttcgg
    16381 ctcgtttgtt aaatacagtt acattagata atgaaagttg attggccatt tcttta
//
LOCUS       DAOOOL010000048        16299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_293_length_16299_cov_98.317471, whole genome shotgun sequence.
ACCESSION   DAOOOL010000048 DAOOOL010000000
VERSION     DAOOOL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16299
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_16299_cov_98.317471"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1479
                     /locus_tag="PKU82_05645"
     CDS             88..1479
                     /locus_tag="PKU82_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ82392.1"
                     /translation="MSTSRREFLRTTSIAGFGTLLIPNSLFALSTLAFKGKKVKIGLI
                     GVGLRGQSNLEILAKRDDVEIIAFADPQSIMLSAAQKILSKNNKPTAKEFSNGEYDYR
                     NLLNDTGIDAVLISTPWEWHKVQGVEALYAKKIVGMEVGGALNLNECWEYVKTHEATG
                     TPLFAMENVCYRRDVMAILNMVRKGIFGELLHGEGGYKHDLRGVLFNDGITPYNSGVE
                     FGTKGYSEARWRTEHYIKRNGELYPTHGLGPVAMMMDINRGNRLLRLSSFASKARGLH
                     DYIVNHPKGGENHPNAKIVFKEGDVVTTQIACENGETILLTHDTSLQRPYNLGFKVQG
                     TKGLWEDFGWGDFDQGHIYFESLMNHSHKWDNSEKWLKEYDHPLWKRYEHLAEGAGHG
                     GMDWFLIHTFIECIKNNVEFPMDVYDLATWYSITPLSEKSIAQGGKVMNIPDFTKGKW
                     KTRKPVFGFSDEY"
     gene            complement(1587..2621)
                     /locus_tag="PKU82_05650"
     CDS             complement(1587..2621)
                     /locus_tag="PKU82_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOZ82393.1"
                     /translation="MIYKFSLIEKILLSNDIIPHPFADASFSVGLSLALGSAIKLKIA
                     DQLSTDFKEVRQIAKEASVSEVGAELTLDCLDALGYVQKKGSQYAFTKRGFKNLSTQS
                     PDNFRYFILFCDNLYKGYLSLDETIRLGKSAKADLLSMTDYDWELFSRAMIDISKTNY
                     KEVVGKIPVSKSATKLLDLGGLHGLYSIELCKRNPSLKATIVDLPPVKKYADECIAKY
                     NASGNVSFLPADFMKDDIPMGVDVILAFNIIHGFNPSENEILAKKVFDSLNAGGIFII
                     LDQIKGIGGNSQMSKATTSYMALNLLHEAGGKTYSKSEIDIYTSKVGFRKTELKKLHA
                     PGFGLIICYK"
     gene            complement(2705..3514)
                     /locus_tag="PKU82_05655"
     CDS             complement(2705..3514)
                     /locus_tag="PKU82_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ82394.1"
                     /translation="MTKSSNIYIPNNEVEKTFIKCIWRLSEYDIQERKEIILPKGTVE
                     IIFNFSDKINYFSPSYKIEKSLPDVFVNGINFKPFELTKTKQQKFLGVQLNSIGLRLL
                     FKIPSFELNNIVCDGNDICKQLSNLANDLNGEEEFSQQVKIILSWIRNTVSFNQIKYA
                     TSRAEKLTSCICYNNLTVKKLSKEIGLSERQLSRFSNDWLGMSTEQFIHYRKYLNCLN
                     LLHQSKQNLTALGMQAGYYDQSHFIHQFKSFTDMTPRQYREAKTDLPGHIY"
     gene            3781..5703
                     /locus_tag="PKU82_05660"
     CDS             3781..5703
                     /locus_tag="PKU82_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ82395.1"
                     /translation="MIKQFLVLIFAFFVSAAFAQKQTYTSTSSRAVKNIEQALKYYDS
                     RQTAKAISETENAIKADPNFIEAYMLLGDLQADNNNIPAAIEAYKKAVAINPRFFPNN
                     FYALGRYELESGRYDDALVHFNEFVKMETAKPDKKEEAKNLIASCEFAKDALQHPVPF
                     SPVNAGAGINSDMDEYFPSITVDSKRMLFTRKIKSDIYEWQEDFYISTLQQDSSWGKA
                     KNVGAPVNTELNEGAPSYSADGQLIFFTACDRPESKGSCDIYYSRKFGDVWGKPINLG
                     SPVNTGAWESQPSFSSDGKTLYFVRGSNSRTNDQDIFMSEVSEKGWSTPVKLNNNINT
                     AGKEEAVFIHPDNQTLYFVSDGWPGMGGSDIFMSKRQADGQWGKAVNLGYPINTFANE
                     AGLIVSPSGDKAYFSSNREGGLGGLDIYSFDLYDKIKPDPVSYAIGYVYDDETKKPAD
                     AKFEIIDIESGKTVVESYSDKNTGEFMVTLPYGKNYMLNVSKRKYLFYSDHFEMDKPS
                     DIKNAKQLIVRLKPVKDGSTMALNNIFFDTNSAELKPESKTELNKLVSFIKSNAIAKL
                     EIGGHTDNVGDDKANMLLSDKRAKSVCDYLITSGIEASRLISRGYGETKPVSDNTTEE
                     GKAKNRRTEITILSVH"
     gene            5801..7369
                     /gene="dnaB"
                     /locus_tag="PKU82_05665"
     CDS             5801..7369
                     /gene="dnaB"
                     /locus_tag="PKU82_05665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOZ82396.1"
                     /translation="MEENVEAGRSAKKKVAPKSLTNQQIALGLGKIPPQAVDMEEAVL
                     GALMLEKNALTAVIDILHTEAFYKEAHQKIFKVIHDLFHKAEPIDILTVTHALKKSGD
                     LELVGGAFYITQLTNRIASAANIEYHARIILQKFIQRELIRISSETIKSAYEDTSDVL
                     KMLDDAEKNLFSIAEGNIRRNYENMNSLITKAIREIEIASKQTQGITGVESGFTDLDR
                     ITAGWQKSDLIIVAARPGMGKTAFTLSLAANAALQFNRPVAFFSLEMSSVQLVNRLLS
                     SQAEITSEAIRKGQLAEHQWQQLNSKIAPLSDAPIFIDDTPALSVFELRAKCRRLVAE
                     KKVQLIIIDYMQLMVAGTDNRNGNREQEISTISRSLKAIAKELEVPIICLSQLSREVE
                     KRGTASKRPQLSDLRESGAIEQDADMVMFIYRPEYYGMDVDDEGHSTKNTAEIIIAKN
                     RNGSLNTVKLTFIGQYTKFKDRELENQTPQDKAIYGYTDESNKVRIPNPNVEAENRYT
                     RGSKMNDMPDEDPF"
     gene            complement(7376..8278)
                     /gene="rfbD"
                     /locus_tag="PKU82_05670"
     CDS             complement(7376..8278)
                     /gene="rfbD"
                     /locus_tag="PKU82_05670"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOZ82397.1"
                     /translation="MLRILLTGSNGLLGQKIVYAAIKHSDIQLLATSKGENRLHTKAG
                     YDYVSMDITNADEVTAIVENFKPHCIINTAAMTNVDACEKDQENCWKMNVEAVRTMTE
                     LCQLHDIHFIHLSTDFVFDGEKGNYSEEDAPNPLSHYARSKFESEKVVQQAEIDSAIL
                     RTIIIYGVVDDKQRSNVVIWTINSLRQQKNINVISDQMRSPTLAEDLADACLQAALKR
                     ATGIYHVSGSETMSILEMVNRTADFFQLDKKYIHPITTAQLNQPAKRPLLTGFNIDKA
                     KRELNFQPHTLQQGLEIVKSQLEN"
     gene            complement(8291..10594)
                     /locus_tag="PKU82_05675"
     CDS             complement(8291..10594)
                     /locus_tag="PKU82_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ82398.1"
                     /translation="MTIKRFFLAAFCMLAVSLVNAQTATIKGFVYAAENGEPVPFASV
                     FLKGTTFASFTDINGYYSMVKIPPGKYRVMATTLGYDTAFADVDLVADEMENRKLVMK
                     KKNVELKQVEVSAERQTKKTEVKIAVESVTPQEIKSLPSLGAEGDLVQYMQVVPGVVS
                     SGDQGGQLYIRGGTPVQNKVLLDGMVIYNPFHSIGLFSVFDTDILKNTDVYTAGFGAQ
                     YGGRISSVMDITTRDGNKKRIGGKVSAGTFLSKVLLEGPIKKAKDENDGTASFILSVK
                     NSYLDQTSKSIYKYASDDGLPYSFNDIYGKVVVNSASGSKVSFFGFNFQDKVNYSKVA
                     KYDWSSSGGGMNFVLVPGASKVLLRGNLAFSSYKIKLTEAEEKPRSSLVNGFNMGFNW
                     LYFLNNDEINYGIEVQGFKTDFEYFNYAKRQINQTENTTEIGIFFRYKKIINKLIIDP
                     SVRLQYYASISEFSPEPRLGIKYNLSDNIRLKAAGGLYAQNLIAANSDRDVVNFFYGF
                     LSAPDDLPSEFDGKSVKKKLQLARHAVVGVEYDISKHFDINIEGYIKDFNQLTNINRD
                     KVYDDTANNFDKPDSVKKSFIIEKGFTQGVDVVLKYEYRRMYAWLVYSLGFSTRNTGN
                     YEYAPHFDRRHNINIIGSYKLGKKLDWEVDARWNFGSGFPVTLNQGFYPYLNFQDGLA
                     TDYTQNNGEMGVLYGPLNESRLPTYHRLDISIKKIFYLARHSELEVVASVVNVYNRKN
                     VFYVDRIRHNVVNQLPIMPSLGASLTF"
     gene            complement(10600..11004)
                     /locus_tag="PKU82_05680"
     CDS             complement(10600..11004)
                     /locus_tag="PKU82_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOZ82399.1"
                     /translation="MQFWLVKSEPFKYSWEQFVADGKTFWDGVRNNAARLHLLAMKKG
                     DQVLFYHSNEGLAVMGIAKVEKTAYTDPTADDTRWVGVELTPFKALKKPVTLAQVKDD
                     ARLKDIALVRIGRLSVMPLKKEEFNVILELSK"
     gene            complement(11055..11642)
                     /gene="purN"
                     /locus_tag="PKU82_05685"
     CDS             complement(11055..11642)
                     /gene="purN"
                     /locus_tag="PKU82_05685"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOZ82400.1"
                     /translation="MNKKRIAILASGSGTNAENIAAYFKNNEYAEVALILTNVATAGV
                     IVRAQKLNIPCLFMDKKTVSDGRLMLDLLKQHSIDLVVLAGYLKLMPAEVVSTFQHRM
                     INIHPALLPLFGGKGMYGHNVHEAVLQAGVAETGITIHYVNSRFDEGEIIFQKSMPVL
                     PDDTAERIASRIHRLEYEHYPSVIEHLAKGLFKDN"
     gene            11781..12602
                     /locus_tag="PKU82_05690"
     CDS             11781..12602
                     /locus_tag="PKU82_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HOZ82401.1"
                     /translation="MYFCTNIKLLRKRKKRTQEVASLALGFSRSTLNSYENGAIANPT
                     VEALINFSNYFKISIDTLLRVDMSKLSDLQLRELELGYDSYVRGTKLRVLATTVDSRN
                     RENIELVPVKAKAGYTAGYNDPEFIRNLPTFQLPFLSHDRKYRTFQISGDSMLPIPDK
                     SFVTAEFVENWMEIKDGNAYIILTHDDGIVFKVVYNHISSNKKLLLKSLNPAYKPYEV
                     KINDVKEVWRFVNYISNEMPDTGLQNPKLSNTVARIQNEMNKIKDLLGVQDVSKE"
     gene            complement(12643..13068)
                     /locus_tag="PKU82_05695"
     CDS             complement(12643..13068)
                     /locus_tag="PKU82_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ82402.1"
                     /translation="MKKVTGIGGVFFKCKDPNKMREWYKQNLGLKTNPYGAVFEWFQG
                     ADNSKKGFTTWSPFDDKTKYFEPSSKEFMINYRVENIEALVAGLRNSGVNVVDEIETY
                     DYGKFVHILDPEGNKIELWEPNDVEYEKMGKEMNSETTK"
     gene            complement(13128..13619)
                     /locus_tag="PKU82_05700"
     CDS             complement(13128..13619)
                     /locus_tag="PKU82_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOZ82403.1"
                     /translation="MPSFDIVNEIDIQKLDNAINVAKKEIVNRYDFHGSKTEIDLDKK
                     EMQLKIVTEDQMRMDAIRDIIISRSMKQGVDPKCFDFGKEQYASGNMVRKDVKIKKGI
                     DKETAKTIVKYIKETGMKVQASIMDEQVRVTGKKIDDLQQVIAEVKNKPVELPLQYVN
                     FRS"
     gene            13718..15997
                     /locus_tag="PKU82_05705"
     CDS             13718..15997
                     /locus_tag="PKU82_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82404.1"
                     /translation="MTQYQKMKINNISHVLRHAMLCLIVFATANTTHAQNYSKYTSPK
                     VFVNSQAFKNPWSGGINSPLYSTIDLNNDGIKDLFMFDKQFGAAYPGSYKIRTFINQG
                     TAGQVDYIYAPFYERFFPQNLHDWVILVDYDCDGNEDIFTYSYSGGMEVYHNDVTPGN
                     NLSFSLTYPLVHSLFLGGAYANLYVSNVNIPVLHDVNGDGDLDVLTFTLLGGTIEYHE
                     NYAKELYNRCDTLVYRQVRQCYAQVGLSAVSNTAILDFNNCFFLAPPPLPDSLIKTPR
                     HNGSCMIGYDQDGDGDLDLINGDILGNNLLYLENSGIPGVSDSIHLQDTLFPSYNVPA
                     VYKTFPAPFYFDADNDGAKDLIVSSCTEYLTEDYQNSWFYKNVGSNTNNQFQFVKKRF
                     LTDEMIDVGSGAYVRFFDVDNDGKKDLIIGNFGYFTQDSASGHYESALSYYHNITTGA
                     LPEFEFVTDDFNNLRSLGRTGLNATFGDLNGDGHDDMILGDKDGILHYYLNNGTTPAT
                     FTLAPNGFNFQNIDVGTNSFPFLADINNDGLLDLIIGERYGYIYYYQNTGSSTNPTFT
                     YVTNKLGNVFVGNAQSFYGFSYPHFYKENGVSKLLVSNENGYIQKYDNIDGNLGGTFN
                     QVSTSMFSVFEPYQTSFDMADLNGDGMDEVITGCYAGGLSIYSKNLPVGIAENLHPEK
                     EITVYPNPAKDHIVFELNKNNPHNGKYQLSDISGKVVMEGSFQSYINNISLEGLTKGI
                     YVLQVTESNHFSSHKIVKF"
     gene            15999..>16299
                     /locus_tag="PKU82_05710"
     CDS             15999..>16299
                     /locus_tag="PKU82_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82405.1"
                     /translation="MRNFCVFLFSLLLINTTAFSQAFVKYTSPIVIKNGQPLRNAWAG
                     GFNSAMFQAIDMNNDGKKDLFAFEKQSESTYPGVFKIRTFINYGSAGDIDYRYDPF"
BASE COUNT         5170 a         3052 c         3029 g         5048 t
ORIGIN      
        1 agcagctttt tttattgctg cttgattttg aacattgagt ttaaaagttg tttttattaa
       61 gtttgtgagt aataaaaata aaatccaatg agcacctcca gacgagaatt tttacgcaca
      121 acttcaatag ccggatttgg cacattacta attcctaatt ctttatttgc cttatctaca
      181 cttgcattta aaggcaaaaa agtaaaaata ggcttaatag gtgttggcct cagaggtcag
      241 agtaatttag aaattctggc aaaaagagat gatgtagaaa ttattgcatt tgccgatccg
      301 caatccatca tgctttccgc agcacaaaaa atattatcga aaaataacaa gccaactgca
      361 aaggaatttt caaatggaga atatgattac cgcaatctgt tgaatgatac cggtattgat
      421 gctgttttaa tttctactcc gtgggagtgg cataaagtgc aaggtgtaga agccttgtat
      481 gccaagaaaa tagtaggaat ggaagttggc ggtgccctaa acctgaatga atgttgggaa
      541 tatgtaaaaa cacatgaggc aacaggcaca cctttgtttg ctatggaaaa tgtttgttat
      601 cgcagagatg tgatggctat tttaaatatg gtcagaaaag gaatttttgg agaattgctt
      661 catggcgaag gtggctataa acatgacttg agaggcgtgt tgtttaatga tggtattaca
      721 ccttacaatt caggagtaga attcggaact aaaggttaca gtgaagcaag gtggaggaca
      781 gaacattaca taaaaagaaa tggagagtta taccccacac atggcttagg ccctgtagca
      841 atgatgatgg acattaaccg tggtaataga ttactgcggc tttcatcttt tgcatctaaa
      901 gcaagaggct tacatgatta tattgtaaat catccaaagg gtggagaaaa ccatcctaat
      961 gcaaagattg tatttaaaga gggtgatgtg gtaacaacac aaattgcctg cgaaaatggt
     1021 gaaacaattt tattgacaca tgacaccagt cttcaacgac cttataattt aggttttaaa
     1081 gttcagggaa caaaaggact ttgggaagat tttggatggg gtgattttga ccaggggcat
     1141 atttactttg agagcttaat gaaccattcg cacaaatggg acaattcaga aaaatggtta
     1201 aaagaatatg atcatccttt atggaaacga tacgaacatc ttgccgaagg cgcagggcat
     1261 ggaggcatgg attggtttct gatacataca ttcattgaat gcattaaaaa taatgtggaa
     1321 tttccaatgg atgtgtatga cttggcaaca tggtattcta ttacaccatt aagtgaaaaa
     1381 tcaattgcgc aaggaggcaa ggtgatgaac attcctgact ttaccaaagg caaatggaaa
     1441 accagaaagc ctgtgtttgg ctttagtgat gaatattaat ttatttcaaa acccaatcaa
     1501 agtagcgcaa attgtatctt ttcaactaac ggtattacca tttacgaagt agccttctgt
     1561 aaaataactt accttaagaa atctccttat ttatagcaaa ttatcaatcc aaaacccgga
     1621 gcatgtagtt tttttaattc tgtcttcctg aaaccaactt tacttgtgta aatatcaatt
     1681 tctgacttgg aataagtttt tcctcctgcc tcatgtagta aatttaaagc catataagaa
     1741 gtggttgcct tcgacatctg agagtttcct ccaatacctt taatctgatc aagaataata
     1801 aaaatgcctc ctgcattcag tgaatcaaat accttctttg ctaatatttc attctctgaa
     1861 ggattgaaac cgtgaataat attaaatgcg agaataacat caacacccat tggaatatcg
     1921 tctttcataa aatctgcagg aagaaaagaa acatttcctg aagcattata cttggcaata
     1981 cattcgtcag catatttctt taccggaggc aaatcaacaa tagttgcttt aagacttgga
     2041 tttcgtttac agagttcaat actgtataag ccatgcaagc cgcccaggtc aaggagtttt
     2101 gttgcagact ttgaaaccgg aattttgccc actacttctt tataattcgt tttagaaata
     2161 tcaatcattg ctctcgaaaa caactcccaa tcataatctg tcatactcaa taaatcagcc
     2221 ttagcacttt tacccaatcg aatagtttcg tctaagctta aataaccttt atacaaatta
     2281 tcacagaaca aaataaaata cctgaaatta tcaggagatt gcgttgacaa attcttaaac
     2341 cctctttttg tgaaagcata ctgagaaccc ttcttctgaa catatcctaa agcatctaaa
     2401 caatcaagag tcaactctgc tcctacctca ctcacacttg cttcttttgc aatttgtctc
     2461 acttctttaa agtctgtaga gagttgatct gcaattttca gctttatagc acttcctaac
     2521 gccagagaca gccctacgga aaaggatgca tcagcaaagg gatgcggaat gatgtcgttt
     2581 gacaaaagta ttttttcaat gagtgagaat ttataaatca tgatttagct gtttagaaaa
     2641 agttaacagc aaaattcaaa aagaaataaa aataaaaatt gtacaattcg gacattccat
     2701 gtgcttaata aatatgtcca ggcaaatctg tctttgcttc tctgtattgg cggggcgtca
     2761 tgtcagtaaa tgatttaaac tgatgaataa aatgtgattg gtcgtaatac cctgcctgca
     2821 tgcccaatgc tgtgaggttt tgttttgatt ggtgaagcag atttaagcaa ttaagatact
     2881 ttctgtaatg aataaattgt tctgtgctca tccccaacca atcattcgag aacctgctta
     2941 gttggcgttc tgacaaacct atttctttac ttaacttttt tactgtgaga ttattataac
     3001 aaatacaact tgttaacttt tcagcacggc ttgttgcata cttaatttga ttaaaagaaa
     3061 ctgtgtttct aatccacgat aaaataattt taacctgttg actaaattcc tcctctccat
     3121 ttaaatcatt agcaaggtta ctgagttgtt tacaaatatc atttccatcg caaacaatgt
     3181 tattcaactc aaaagacgga attttaaaaa gtaatcgcag cccgatactg ttcaattgta
     3241 ctcccagaaa tttttgttgt ttggttttag taagttcgaa tggcttaaaa ttaattccat
     3301 ttacaaagac atcaggtagt gatttttcaa ttttatatga aggactgaag tagttgattt
     3361 tatcagaaaa attaaaaata atttcaactg tacctttggg aaggataatt tcttttcttt
     3421 cctgaatatc atattctgaa agtcgccaaa tacattttat gaatgtcttt tcgacttcat
     3481 tgtttggaat ataaatatta ctggactttg tcactttaat ttgaatgaat tatcattaac
     3541 ataagtattc aaataagaac atttaaaatt tcattttaaa actcaaaagt aataagaaaa
     3601 cttcctcata aatcaccatt ctgaatactt ttatgcggta ttctgattct attacaacta
     3661 ctatcaactc aatggcgata tcagtttctg ataaaataat ttttgtgaca cactgattca
     3721 caccatactt tcatataata agtgcgttct gtttttatat ttgcaataaa ttatcaggta
     3781 atgattaaac aatttttggt tttaatattt gcattttttg tcagtgcagc atttgcacaa
     3841 aaacaaacct atacttcaac ttcgtcaaga gctgtgaaaa atattgaaca ggctttaaaa
     3901 tattatgatt cgaggcagac agccaaagca atttccgaaa cagaaaatgc cattaaagct
     3961 gatccgaatt ttattgaagc atacatgctt cttggcgatt tgcaagccga caacaataac
     4021 atacctgccg ctattgaagc ttataaaaaa gctgttgcaa taaaccctcg ttttttccca
     4081 aacaattttt atgcattagg aaggtatgaa ttggaatcag gaagatatga tgatgcattg
     4141 gttcacttca atgaatttgt gaaaatggaa actgcaaaac ctgacaagaa agaagaagca
     4201 aaaaacctga tagcatcctg tgaatttgca aaagatgcac tgcaacatcc tgtacctttt
     4261 tcaccggtaa atgcaggtgc aggtataaat tcagatatgg atgaatattt tccttctatt
     4321 acagtagata gtaaacgaat gttatttaca cgaaagatta agagcgatat ttacgaatgg
     4381 caggaagatt tttatataag cactttgcag caggacagca gttggggtaa ggcaaaaaat
     4441 gtgggtgcac cggtaaatac agagttaaat gaaggagcac catcatacag tgctgatggc
     4501 caactgatat tttttacggc atgtgaccga cctgaaagta aaggttcatg tgatatttat
     4561 tactccagaa aatttggtga tgtatgggga aagccaatca atcttggctc gccagtgaac
     4621 acaggtgcat gggaatcgca accttctttt tcgagtgatg gcaagacctt atactttgtt
     4681 cgtggcagca acagccgcac caacgatcag gacattttta tgagtgaggt gagtgaaaaa
     4741 ggatggagta caccggtgaa actaaacaac aacatcaaca cagcaggaaa agaagaagcc
     4801 gtatttattc acccggataa tcaaacgtta tattttgttt ctgacggatg gccgggcatg
     4861 ggaggctcag atatctttat gagtaaacgc caggctgatg gtcagtgggg caaagcagta
     4921 aacttaggat atccaataaa tacatttgcc aatgaagcag gattgattgt aagtccttcg
     4981 ggcgataaag cctatttcag cagcaacaga gaaggtggat taggaggttt ggatatttac
     5041 agtttcgact tgtatgataa aattaaacca gaccctgtga gttatgcaat aggatatgtg
     5101 tatgatgatg aaacaaaaaa acctgccgat gcaaaatttg aaatcattga tattgaatcg
     5161 ggcaagacag ttgtagaatc ttattctgac aaaaacacag gagagtttat ggtgaccttg
     5221 ccttatggaa aaaattatat gcttaatgtg tcgaagcgaa aatatctttt ctactccgat
     5281 cattttgaaa tggataaacc atcggatatt aaaaatgcaa agcagttgat agtaagactt
     5341 aaacccgtga aagatggcag caccatggct ttgaataata ttttctttga caccaattct
     5401 gcagaactaa aacctgaatc aaaaacagag ttaaataaac tggtgagttt tattaaaagt
     5461 aatgccattg caaaacttga aattggaggg catactgata atgtaggtga tgacaaagca
     5521 aacatgctgt tgtctgacaa gcgagcaaaa tcggtttgtg attatcttat tacatcaggc
     5581 attgaagcat cgcgattgat ttccaggggt tatggagaaa caaaacctgt gtcagataat
     5641 acaactgagg aaggaaaggc aaaaaacaga agaacagaaa tcactatatt atcagttcat
     5701 taattttttt gcatacaaaa tttttctgtt aaatacgtgt tgaaaattat ttaaaaatat
     5761 tttcggagaa gggggcgtgg caattgacta aatttgctgc atggaagaaa atgtggaagc
     5821 aggcaggagt gcaaaaaaga aagtagctcc caagtcgtta acaaatcaac agattgcatt
     5881 aggattaggc aaaattccac cacaggctgt ggatatggaa gaagcagtat taggcgcatt
     5941 aatgcttgaa aaaaatgcac tcactgctgt tatagatatt ttacatacag aagctttcta
     6001 taaagaagca catcagaaaa tatttaaagt catacatgat ctttttcata aagctgagcc
     6061 aattgatatt ttaacagtta cacatgcttt gaaaaaatca ggcgatttag aattagtagg
     6121 tggtgcattt tatattacgc agttgaccaa cagaattgct tctgcggcta acattgaata
     6181 tcatgcgcgg attatccttc aaaaatttat tcagcgtgag ttgattcgta tttcgagtga
     6241 aactataaag agtgcttatg aagacacatc agatgttttg aagatgctgg acgatgcaga
     6301 gaaaaattta ttttcaattg cagaaggaaa cattcgcaga aattacgaaa acatgaattc
     6361 tcttattacc aaagccatta gggaaataga aattgcatca aaacaaacac aaggcattac
     6421 aggagtagaa agtggcttta ctgacttaga tcgtataaca gcaggatggc aaaaatctga
     6481 tttgataatt gttgctgcaa gaccgggtat gggaaaaaca gcctttaccc tatcgttagc
     6541 agccaatgcc gcattacaat tcaacagacc ggttgcgttt ttctcattgg agatgtcaag
     6601 tgttcagttg gtaaaccgtt tgctttcttc acaggcagaa ataacttcag aagccatcag
     6661 aaaagggcaa cttgcagaac atcaatggca acagctaaac agcaaaatcg ctccgctttc
     6721 tgatgcacct atttttattg atgatacccc ggcattatcg gtttttgaac ttcgtgccaa
     6781 gtgcagaaga cttgttgcag aaaagaaagt acaactcatc attattgatt atatgcaatt
     6841 gatggtggca ggaacagaca atcgtaatgg aaaccgtgag caggaaatca gtaccatttc
     6901 gcgttcatta aaagctatag ctaaagaatt agaagttccg ataatttgtt tgtcgcagtt
     6961 gagtcgtgaa gtagaaaagc gcggaacagc ttctaagcgc ccgcaattga gcgaccttcg
     7021 tgagtcagga gctattgagc aggatgctga tatggtaatg tttatttacc gcccggaata
     7081 ttatggtatg gatgtagatg atgaaggcca ttcaacaaaa aatactgcag aaattattat
     7141 tgccaaaaac agaaatggtt ctttaaatac cgttaaactc acctttattg gtcaatatac
     7201 taagtttaaa gaccgtgagc tagaaaatca gacaccacaa gataaagcaa tatatggcta
     7261 tactgatgaa agcaataagg ttagaatacc taatccgaat gttgaagcag agaatcgtta
     7321 cacacgtggt tcaaaaatga acgacatgcc tgacgaagat cctttttagt ctgacttaat
     7381 tttctaattg actctttaca atttccaaac cttgctgcaa ggtgtgaggc tgaaagttta
     7441 attcacgttt tgctttatca atattaaaac ctgttagcaa aggacgtttt gcaggttgat
     7501 tgagttgagc agtagtgatg ggatgaatat attttttatc cagctgaaaa aaatctgctg
     7561 tacggtttac catttcaaga atgctcatag tttcactgcc ggatacatga tatattcctg
     7621 ttgcgcgttt taatgcagcc tgcaggcagg catcagcaag atcttcggca agtgtaggtg
     7681 agcgcatctg atcgctgatg acattgatat ttttttgctg acgcagtgag tttattgtcc
     7741 agataacaac attacttctt tgcttgtcat ccactactcc ataaatgatg attgtacgta
     7801 aaatagcaga atctatttct gcttgctgca caactttttc cgattcaaat ttactgcggg
     7861 cataatgact caacggattg ggggcatctt cttcgctgta gttgcccttt tcaccatcaa
     7921 aaacaaaatc ggtactcaga tgaataaaat gtatgtcatg aagttgacac agttcagtca
     7981 ttgtcctgac tgcttctaca ttcatcttcc agcaattttc ctgatctttt tcgcaggcat
     8041 caacattggt cattgcagca gtgttgatga tacaatgcgg cttaaaattc tcaacaatgg
     8101 cagttacttc atcagcattg gtaatatcca ttgaaacata gtcatagcct gcttttgtat
     8161 gcaaacggtt ttcaccttta gaagttgcaa gcagttgtat gtcgctatgc tttatagctg
     8221 cataaacaat tttctgcccc agcaatccat tacttcctgt caataaaata cgcagcatga
     8281 cattgcaaaa ttaaaacgtg agcgatgctc ccaatgatgg cattattggc aattgattta
     8341 ccacattgtg tctgatgcga tcaacataaa acacattttt acgattataa acattgacaa
     8401 cacttgccac cacttccagc tcactgtgtc gggcaagata gaaaattttc ttgattgaaa
     8461 tatcgagacg atgataggtc ggtaatcgcg actcatttaa tggtccgtag agcacaccca
     8521 tttcgccatt gttctgagta tagtcagtag caagtccatc ctgaaaattg agataaggat
     8581 aaaatccctg atttaaagtt acaggaaaac ctgatccgaa attccagcgg gcatcaactt
     8641 cccagtcgag ttttttacct aacttataag agccgatgat attgatatta tgtcgtctgt
     8701 cgaaatgtgg agcatattcg tagttgcctg tattgcgtgt gctgaatccc aaagaataaa
     8761 cgagccatgc atacattctt ctgtattcat atttcaaaac aacgtcaaca ccttgtgtaa
     8821 atcctttttc gatgataaaa cttttcttaa cggagtcggg cttgtcgaag ttgtttgcag
     8881 tgtcatcata aaccttgtcg cggttgatat tggtaagctg attaaaatct ttaatgtagc
     8941 cttcgatgtt aatatcaaaa tgtttgctga tatcatattc aacaccaaca actgcatgac
     9001 gtgcaagctg cagttttttc ttaacggatt ttccatcaaa ctcagaaggt aaatcatcag
     9061 gtgcagaaag gaaaccatag aaaaagttta ccacatcgcg gtcgctgttg gcagcaataa
     9121 gattttgagc atacagtcca ccggcagctt taagtctgat gttgtcgctc aggttgtatt
     9181 taattccaag gcgaggttca ggagaaaatt cagagatgga tgcataatac tgcaagcgaa
     9241 cggaaggatc aatgatgagt ttattgatga tttttttgta gcgaaagaag attccgattt
     9301 cagtagtgtt ttctgtctga ttgatttgtc tcttggcata attaaaatat tcgaaatcgg
     9361 ttttgaagcc ttgcacttca ataccataat taatttcgtc attatttaaa aagtaaagcc
     9421 agttaaagcc catgttaaat ccattcacta agctggagcg tggtttttct tcggcttctg
     9481 taagcttaat tttatagctg gagaaagcga ggttaccacg cagcaatact tttgatgcac
     9541 cgggcaccaa cacaaagttc attccaccac cggaagaact ccagtcgtat tttgctacct
     9601 tgctgtaatt tactttatct tgaaaattaa atccgaaaaa gctaacctta cttccgcttg
     9661 cactgttgac aaccactttt ccataaatat cattgaaaga atacggcaag ccgtcatcgg
     9721 aagcatattt gtaaatactt tttgaagtct ggtcgaggta ggaatttttt actgaaagaa
     9781 tgaaagacgc tgttccgtca ttttcgtctt tagctttctt tatcggacct tctaacaaaa
     9841 ctttagaaag gaatgtgcct gcagaaactt ttccgccaat acgtttttta tttccatcgc
     9901 gtgtggtgat gtccatcact gatgaaatgc gtccaccata ttgcgcacca aaacctgcag
     9961 tataaacatc ggtatttttt aaaatgtctg tatcaaacac actaaacaag ccaatgctat
    10021 gaaacggatt gtagatgacc ataccatcga gcaacacttt gttttgtaca ggagttcctc
    10081 cgcggatata caattgccct ccctgatcgc cactgcttac aacaccgggc actacctgca
    10141 tgtactgtac caaatcacct tcggcaccaa gacttggcag agatttaatt tcctgaggtg
    10201 ttacagactc tactgcaatt ttaacttctg ttttttttgt ttgtctctct gccgatacct
    10261 caacctgttt caactccaca ttttttttct tcatcactaa tttacggttt tccatttcat
    10321 cggcaacaag atcaacatct gcaaaagcag tgtcgtagcc taatgttgtt gccattacgc
    10381 ggtattttcc tggaggaatt ttcaccatgg aataataacc gttaatgtct gtaaaagagg
    10441 caaaggttgt tcccttcaga aaaacacttg caaagggaac aggctcacca ttttctgcag
    10501 cataaacaaa ccctttgatg gtggctgttt gtgcatttac cagagaaacg gcaagcatac
    10561 agaaagcagc taaaaaaaat ctttttatcg tcataaaaac tatttactta attctaaaat
    10621 tacattaaac tcttcttttt taagtggcat taccgacagg cggcctatgc gcaccaatgc
    10681 aatatctttc aaccgtgcat catcttttac ctgtgccaat gttacaggct tcttcaatgc
    10741 tttgaatggt gtcaactcca cacctaccca acgtgtatcg tcagcagtgg gatcggtata
    10801 tgctgtcttt tcaactttag caatacccat aacggcaagg ccttcattgc tgtgataaaa
    10861 caaaacctga tcgccttttt tcatggcaag taaatgcagc cgtgcagcgt tgttgcgcac
    10921 accgtcccaa aatgtttttc catcggcaac aaactgctcc cacgaatatt taaacggctc
    10981 tgattttacc agccaaaact gcatactatt tttaaagtcg gacaaagaaa taaaaataat
    11041 cgaatacgga tttgttaatt atccttaaat aaaccctttg ccaaatgctc aattacggat
    11101 gggtaatgct catattcaag acgatgaata cgtgacgcta tacgctctgc cgtgtcgtca
    11161 ggtagcacag gcatactttt ctgaaaaata atttcaccct cgtcaaagcg gctgtttacg
    11221 tagtgaatgg ttatgcccgt ttctgcaaca cctgcctgca acactgcctc atgaacgttg
    11281 tgaccataca tgcctttgcc tccgaaaagt ggcagcaatg caggatgaat atttatcatg
    11341 cgatgctgaa aagttgaaac aacttcggca ggcatcagct ttaggtatcc ggcaagcaca
    11401 accaaatcaa tgctatgttg ctttaacaaa tcaagcatca gacggccatc gctaacagtt
    11461 tttttgtcca taaaaagaca aggaatgttt aacttctgtg cgcgtactat cacacctgcc
    11521 gttgccacat tggtgagaat caatgccact tctgcatatt cattgttctt aaaataagca
    11581 gcaatatttt cggcattggt gcccgacccc gatgctaata tggctattcg ctttttgttc
    11641 attgcacaat tatgagcgtg taaaaatagc attctgtaaa tgataggctt agatattttt
    11701 ttggcattat tacaatcatt catttggtta ttatatagcc tgtatattaa atttgtagta
    11761 aattcattaa accacataac atgtatttct gcaccaacat aaaactactt cgcaaacgca
    11821 aaaagcgtac acaggaagta gcatcacttg ccttaggatt ttcgcgctct acattaaaca
    11881 gctatgagaa tggcgctatt gccaacccta cagttgaggc actgataaat ttctctaatt
    11941 attttaaaat ttctatagac accttgctgc gtgttgacat gagtaaactc agcgacttgc
    12001 aactgcgcga actggagtta ggctacgact cctatgtgcg tggcacaaag cttagggttc
    12061 ttgcaacaac ggttgacagc agaaaccgcg aaaacattga gttggtgcct gtgaaagcta
    12121 aagccggata tactgcaggc tacaacgacc cggagtttat acgcaaccta cctacatttc
    12181 aactgccctt cctttcgcac gacagaaaat accgcacctt tcagattagt ggcgacagca
    12241 tgctgcccat accggacaaa tcgtttgtta cggcagagtt tgtagaaaac tggatggaga
    12301 taaaagatgg caatgcctac atcatactca cacacgatga tggcatagtg tttaaagtag
    12361 tttacaatca catcagcagc aataaaaaat tattgcttaa atcattgaat cctgcctaca
    12421 aaccttatga ggtaaaaatt aatgatgtaa aagaagtgtg gcgatttgta aactacatca
    12481 gcaatgaaat gcctgatacg ggattgcaaa atccaaaact atcgaacacg gttgcacgca
    12541 tccaaaatga aatgaataaa atcaaagacc tgctgggtgt gcaggatgtt agtaaagagt
    12601 agttttttga gagagaggaa agaacggctt agccaataaa aatcacttcg tagtttcact
    12661 attcatctcc ttacccatct tttcatactc cacatcattg ggctcccata gttcaatttt
    12721 atttccttcc ggatccagta tgtgaacaaa tttaccgtaa tcgtaggttt ctatttcatc
    12781 aacaacattt actcctgagt tacgtaaccc ggcaacaagt gcctcaatgt tttcaactct
    12841 gtagttaatc ataaattcct ttgatgaagg ttcaaaatat tttgttttat catcaaacgg
    12901 gctccatgtt gtaaaacctt ttttagagtt atcggctccc tgaaaccact cgaaaaccgc
    12961 tccgtaagga ttggttttta atccaagatt ttgcttatac cattcacgca ttttattagg
    13021 gtctttacac ttaaaaaaaa cacctccaat acctgttacc tttttcataa ttctttgatt
    13081 tgtttttcag aataaaattt ttcaatctaa tacaatcaac aacttgctca cgacctgaaa
    13141 ttaacatatt gtaatggcaa ctccacaggt ttgtttttta cttcggcaat aacctgttgc
    13201 aagtcatcaa tttttttgcc tgtaacgcgc acctgctcat ccatgataga tgcctgcacc
    13261 ttcatccctg tttctttaat atatttcaca atggttttgg cagtttcctt atcaattcct
    13321 ttttttattt tcacatcttt acgaaccata ttgccggagg catactgctc tttgccaaaa
    13381 tcgaaacatt tcgggtcaac cccttgcttc atgcttcggc tgatgatgat gtcgcgaata
    13441 gcatccatac gcatctgatc ttcagtaaca atttttaact gcatttcctt cttgtctaaa
    13501 tcaatttctg tcttagaacc atgaaaatcg tagcggttaa caatttcctt ctttgccaca
    13561 ttaatggcat tgtctaattt ctgaatgtct atttcattaa cgatatcaaa tgatggcatg
    13621 actgactatt ttatgttcat gcaaaagttg taataattcg tgaataatct gattatgacg
    13681 ctattttttt tctacttttg aatgtccttt cagggtcatg actcaatatc agaaaatgaa
    13741 aatcaacaac atcagccatg ttttaagaca tgccatgctt tgccttatcg tatttgcaac
    13801 tgccaatact actcatgcac agaactacag caagtacact tcgcctaagg tgtttgtcaa
    13861 tagtcaggct tttaaaaatc cctggtcagg tggcatcaac tctccacttt acagcaccat
    13921 tgatttaaac aatgatggca tcaaggattt gtttatgttc gataaacaat ttggtgcagc
    13981 ctatccgggc tcctataaaa tcagaacttt tatcaatcag ggaacagcag gacaggttga
    14041 ttatatctat gctccatttt acgagcgttt ttttccacaa aatctgcatg actgggtaat
    14101 attggttgac tatgattgcg atggtaatga agatattttt acttactcct actcaggtgg
    14161 tatggaagtt tatcataatg atgttactcc cggaaataat ctttctttca gtttaacata
    14221 ccctcttgtt catagtttat ttttaggtgg tgcttatgct aacttgtacg tgagcaatgt
    14281 taacattcct gttttgcatg atgtaaatgg tgatggtgat ttggatgttt taactttcac
    14341 attgcttggt ggcacaattg agtatcacga aaattatgca aaagaacttt ataaccgttg
    14401 cgacacttta gtctatcggc aggtgcgaca gtgttatgca caagtaggtt taagtgccgt
    14461 ttccaataca gctattcttg attttaataa ttgtttcttt ctggcaccgc caccactacc
    14521 cgattcactg ataaaaacac cacgtcacaa tggctcctgc atgataggat atgatcagga
    14581 tggtgatggt gaccttgact tgataaatgg tgatattctt ggcaacaacc tgctttatct
    14641 tgaaaacagc ggcattccgg gagtgagtga ttccattcac ctgcaggaca cactttttcc
    14701 atcgtataat gttcctgcag tttataaaac attccctgcg cctttttatt ttgatgccga
    14761 taatgatggt gcaaaagatt taattgtttc tagttgtaca gaatatctga cagaagatta
    14821 tcaaaactca tggttctata aaaatgttgg atcaaacacc aacaatcagt ttcagtttgt
    14881 aaaaaaacgt tttcttactg atgaaatgat tgacgtaggt tcaggtgcat acgtacgctt
    14941 ttttgatgtt gacaatgacg gaaaaaaaga tttgataata ggcaacttcg gatactttac
    15001 tcaagattcg gcaagcggtc attatgaatc tgcattatct tattatcata acatcactac
    15061 aggtgcacta cctgaatttg aatttgtgac tgacgacttc aacaacctgc gttcattagg
    15121 gagaaccgga ttaaatgcaa catttggaga cctaaacggt gatggtcatg atgatatgat
    15181 tttaggtgat aaagatggta ttttgcacta ctatttgaac aatggtacaa cacctgcaac
    15241 attcactctt gcgcctaatg gttttaactt tcagaacatt gatgtaggaa caaacagctt
    15301 tcctttttta gctgatatta ataatgatgg actcttagat cttatcatcg gtgaacgata
    15361 tgggtatatt tattattatc agaatacggg ctcatcaacc aatcctacat ttacctatgt
    15421 taccaataaa ttaggcaatg tatttgttgg aaatgcacaa tcgttttatg gattcagcta
    15481 tccacatttt tataaagaaa acggtgtgtc gaagcttttg gtttcaaatg aaaacggata
    15541 cattcaaaaa tacgataaca ttgatggcaa ccttggcggc acatttaatc aggtttcaac
    15601 aagtatgttt tcagtattcg aaccttatca gacttcattc gatatggccg accttaacgg
    15661 tgacggcatg gacgaagtga ttactggatg ctatgcaggt ggcctttcaa tttattcaaa
    15721 aaacctgccg gtaggcattg cagaaaacct gcatcctgaa aaagaaatta cagtctatcc
    15781 taatcctgca aaagatcata ttgtttttga gttgaataaa aacaacccgc ataacggcaa
    15841 atatcaacta tccgatattt caggcaaggt agtaatggaa ggctcttttc aaagttatat
    15901 taataacata agtcttgagg gattaactaa aggtatttat gttcttcaag taactgagag
    15961 caatcacttc agttcacata agattgttaa attttaatat gagaaatttt tgtgtttttc
    16021 tattttcttt actgcttatt aatacaacag cattttcgca ggctttcgta aaatacacat
    16081 cgccaattgt aattaaaaat ggtcaaccgc ttcgcaatgc atgggcagga ggttttaact
    16141 ctgccatgtt tcaggctatt gacatgaaca atgatggcaa aaaagatttg tttgcctttg
    16201 aaaaacaatc ggagtcaacc tatcccggtg tttttaagat aagaactttt atcaattatg
    16261 gcagtgcagg ggatattgac tatcgctatg atccttttt
//
LOCUS       DAOOOL010000049        16261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_295_length_16261_cov_55.376342, whole genome shotgun sequence.
ACCESSION   DAOOOL010000049 DAOOOL010000000
VERSION     DAOOOL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16261
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_16261_cov_55.376342"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKU82_05715"
     CDS             <1..544
                     /locus_tag="PKU82_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOZ82406.1"
                     /translation="VYKNLKLSLAGEYQLKNVATVLSAIELLKDSCFVIEKKNVKKAL
                     TDIQQLTGFRGRWQVLGTKPLIIADTGHNVDGIKTVIRQICKISFKRLHIVIGMVNDK
                     DIMPVLKLLPKHATYYFCNAKIPRAMPAKELKNKAIEVGLNGQIYTSVKQALLSAKKA
                     AVAQDLIFVGGSTFTVAEVL"
     gene            656..6040
                     /locus_tag="PKU82_05720"
     CDS             656..6040
                     /locus_tag="PKU82_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ82407.1"
                     /translation="MAVVFLANADQTIFPVNSVWKYLDNGTNQGTTWRDSSFNDAAWS
                     SGTAKFGYGEGDENTVVSYGPNPSNKYITTYFRKAISISNLATFSAFELGVRRDDGVV
                     VYVNGVEAWRDNINGVVSDTSLAASDASDDGNSIITATIPTSYFVNGNNVIAVEIHQF
                     VVNSSDLSFDMRLTGLETPPPSNVTIFPLNSVWKYLDNGTDQGTTWRDVSFSDATWTT
                     GPGEFGYGDGDEATVVSYGPNPSNKYITTYFRKSVSIADVNAYTSFQIGVKRDDGVVL
                     YVNGVEVWRDNIAGTVSYNTLATADAADDGSSVLTANIPTSYFVNGTNVIAAEVHQFV
                     VNSSDLSFDMSLTGMGNTPPTMPSIVIGSYLNVATPTSIIVRYRTDITAMSKVVYGTT
                     SGVFTDSTVSASPVTEHIVQLSNLQPNTKYYYAVKSGNNFIQNNPAENFFITPPVAGT
                     AKPTRIWVIGDMGFGSVEQNQVRDAYYNYTGSTYTDLWLWLGDNAYNSGTDAEYQNNV
                     FNNRYEKMLRQTVVWPAAGNHEMYASNAVTETGPYYELFNFPTNGEAGGVPSGKKAYY
                     SYNYANIHFVVLESTTASFRTIGGAMMNWLQTDLAANTQKWTVVYFHHPPYSMGSHNS
                     DTETELIEMRQNFVPILEQYKVDLVLCGHSHNYERSFMIKNHFGSENTFNSSNLVDGG
                     SGAGATPYIKTASSNFQGTVYAVAGTGGILEGIQASWPHSAMYSYSSATYGSMVLDID
                     GDTLYAKFLDNTLPNPTIYDQFTIIKQCDQQVTLAPFSNVCSGQSSFALTGGSPAGGS
                     YSGTGVSNGMFNPAVAGIGTHTITYTYTNGFCSDSATSTITVTTGGQIATINPSGNIT
                     LCQNDSVLLSANSGTGLTYQWQLNGSDISGATSQTYTANGAGNYTVAITSSCGTVVSS
                     ATIVSAASSVSISPSGSVNLCSGPVTLSVNTGVGFNYQWKNNGVDINGATNSSLEILS
                     AGNYSLLATDSTGCASLSNIVNATGSAHVISALGSTTICAGGNVTLSAQTGSGFTYQW
                     YRNNGVISGAINSTYKATSHGNYYCRTTAPGCTGNSNIIVVAVINNPTPTISANGSLN
                     ICSGSSVILSTNTFPGVTYQWERNGSAVNGATSQLHTTTLAGTYRVSQTANGCSKKSP
                     SISVKIVAGPTATINANGSVNLCNGQTVTLNANTVTGAAYQWLADGVNIAGETNQSLV
                     VNTSGNYQCRITTTCAALSNVITVTASSMQISISPSTTQNICQGNSVQFSTTNDPGNS
                     YQWNIDGSAIPGAISDSYSANVSGIYSVSITNGCGTQTSQAVTVNVLPVSAVISPDAA
                     QVICSGGSVNFTANSGFGNTYQWNLNGTPINGATNQTFNANAAGNYSVTVSNSCGVYT
                     STSVSVSVGSISATISPDIAQTICSGDSVLLSANPASGISYQWYLDGNAIGGANTATY
                     FAKSQGNYTVNIVSSCGSSTSPSVALAVISLSSSITPAATQIICPGSFVLLSANTGNG
                     LTYQWKKDGVLISGATQSTFLADETGIFTVDVTNSCGTLESNQVTVNAALINSSITPS
                     GTSTVCSGTPQTFSVLPTAGATYQWYRNNVAINNATNVSLNASSSGQYYVKVHLSGCN
                     IVTSNTAILSVQTNTTPVVTVTPGTTLCNGQQATLSSNIYSGVAIQWQHEKVNIPGAT
                     SQTYQASVGGQYRVVHTFNGCVRNSAEKSIKFVSCRSEGYESDLEMHVSAEVFPNPFK
                     EVTNIKLSGFDISSTTITVTNILGEMINRFNPDSENIEWKRNGNAPGIYLIQITDGYN
                     HRITQKIVIE"
     gene            6146..9301
                     /locus_tag="PKU82_05725"
     CDS             6146..9301
                     /locus_tag="PKU82_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOZ82408.1"
                     /translation="MTTKQFLLAFLFCLPLFSKADTTLVSFNDAWKYFDNGYLPATNW
                     NQIMYNDSAWAIGNGEFGYGDGDETTVVSYGGDPNNKFVTTYFRKTISLNNPYDFASY
                     HLDLKRDDGAIVYINGTEVFRSNMTSGSVGFNTPAYSDCVDDGMALQSIVLVPADFLA
                     GVNVIAVEVHQINNVSSDLSFDLQLNAYNNFVPQLLRQPYLQMVADSAITIRWRTNVS
                     CQTAVLYGNDPDSLNFSVSDSANVIDHEIRLTGLSPDTRYFYALGDGTNVLQGDSNNY
                     FKTAPVKGMPKPVRIWSMGDTGINTIDQNNVRDAFLNYNDSLPIDLLYMLGDNAYYSG
                     TDQEYQDAFFQNHYEDILKNIPVFSVAGNHENYTANAITQTGPYYDIFTFPKNGECGG
                     VPSGSEAYYSFDYSNIHFIALETNTLSLLQPGSDMLNWLQSDIAATQQKWKIVVFHNS
                     PYTKGGHNSDTETDLILIRQNIMPILDQHKVDLVLCGHNHVYERSYFMDGHYGLSSTF
                     DSTMVVDSSFGELPQPYLKKSSKNYKGLVVVQAGCSGVLDPIGPQWPHPAMKRYYDQE
                     LGSLLIEIETDTLTVTFVDNNVSSPQVLDEFSVVKECNVVPQLTVPVAKVCVTDDPVQ
                     LSATPIGGFFSGLGVVDTVFNPTVAGQGIHTIAYTYNDIYGCHVTDTKTIEVVGGVPS
                     QPFGIVGSILVCPPSNNEVLSIQSLPDADSYIWSASAGVNLTSSNTDTLIKFDINTVL
                     PQYNISVSALNICGSSIPSLVNITSVKSFPVSLTGNSVICPGDTSVYTVNVFNSLSSY
                     WWQPSEHVFLNGQSDIINTFNTLHVNASVDSSFTEGKICVASKEACLRNNAVSCIDLS
                     TKPPVPGVISGPAAVLGGSNGVVYSIVPVSGATGYFWTVTGDAILTAGQFGNSVTVDF
                     LPSFTGGYVGVKSQSNCDESIEVSKRIKTYIIQVSQNTVSQTRIANEYNQDKIVSVYP
                     NPIGNEMHVKLLLQDASSNVLVVLKDVAGKEVGKWSKKVFSDEDDITLNVSGLSSGVY
                     FLCVTTDNMQWNGVVVKQNDH"
     gene            complement(9295..10716)
                     /locus_tag="PKU82_05730"
     CDS             complement(9295..10716)
                     /locus_tag="PKU82_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ82409.1"
                     /translation="MATNNGVIKTKFAELVHYFNDTTYQVSYFHYSEEDGISNPEFNG
                     GCDPNYLRLNNGYVSLPNVEGLVWFYPEKIEDPRSDYPISIDQFIADDSIYYTSNNIK
                     VPNSVQYVRIEFSNPYWGEPENLFCEYKLEGYNKQWIYLSPNESALEFSNLKSGEYKF
                     TIRKQTGLSKDYTEATIAFHIENKYYETIWFWLAMVGLALLIIIIVARIYAFNIRRKN
                     ILLEKNVVLRTSELQTANNELSESIKIKDKLISIISHDLITPLRFITMVAKKGASEEI
                     EMDNKKHKELLGEIENTTEKLQANAENILNWIKHQNKRIKKQISSVAIGALAEDITEQ
                     FMHLTYNKGTRLISTISHDDIIQSDSNLLSIILHNIISNATKFTENGFIKIYSKQNNQ
                     YEITIEDNGKGMSEQQLTRITKLINKQPLNEEDEQLDNKEGFGLGYIIISELVQILNG
                     KIEVQSKLGLGTKVTISLPLTYQ"
     gene            complement(10799..12340)
                     /locus_tag="PKU82_05735"
     CDS             complement(10799..12340)
                     /locus_tag="PKU82_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82410.1"
                     /translation="MHKLKVLFLVFLFTFFKLSAQNSYSIQNYTIKKGLPDNNVLGLF
                     YEKNGYVWVVYGSGLARFDGINFKKHFTSDAPYLGLYFYQTTNADKIMIEPSGNIFQI
                     KGLTSDTLRKGKINSLNYINIKGTLPDIKTYLELSTPHVNITVDKSWILTPLIMFPLP
                     DNSYIVRTKKGIAHFKGKNKLKELNLDHLQNRKFISANGNIYVFANDSEIYRVDLTSW
                     SLVKCKLKGDLSSCKNYSRKITNTFWDFNNSDPCVQVDYTLYSFFPDSLNANLISTVQ
                     LTNQLPNNCLISSIIYNEDQNLIMIGTDTRGLFVYKKRNFKSLEFANPEPGTNNTYFC
                     QIALNDSTVYTDWNREFTINGGKQSKLNLARNYSENICIDKKGNYWYAENNSLVRFNP
                     KTKAKKYIYYTGDELPLSFFEEGDTMWVGLIKSFGCVVNDTLKHIKILDNDESNSNIF
                     QLLRFEGKLWICNYTGIYKYDTQSGKLDTLPELALKYTYHFKAINNYLLIGTYGRGYY
                     MYKKR"
     gene            complement(12346..12993)
                     /locus_tag="PKU82_05740"
     CDS             complement(12346..12993)
                     /locus_tag="PKU82_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82411.1"
                     /translation="MNAFSDISLLIADDHVIIRRGLKFLLETHFGKFNIVETDSSKGI
                     LDSLEKNNFTHLILDMQLSDGNLLEIFQEIKQQFPLLPILIYSMSPEEIFAKRLNNLG
                     ASGYLNKQSSEEEVVKAMELFFTGRKYVSQKYFDQQHGVQKGKSENPFSNLSERETEV
                     LLLLLKGKSVKEIASHFDLKSNTVATFKARIFDKIGVNNLMDLQNSARAYNIPIV"
     gene            13444..>16261
                     /locus_tag="PKU82_05745"
     CDS             13444..>16261
                     /locus_tag="PKU82_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82412.1"
                     /translation="MLNFNLQAQTTVTIGAGSQSGTQSNSAAGDCGPMYRSTASSAFV
                     YSRHHYLYTQSEISSAGIPSGNLITKLAWYNQNGAGTNSPCIFQIWMKNSALTDVGVG
                     GQNWSTLTSGSTLVYNSSTHSVPATVGWHDIVLSTPFTYTGGAIEISVNFDISGGSSP
                     YTTNGFSWNKDPITGRTLSYVGSAAPGATLPNLRTVRAQIQMTYQSGAPCSAPPTAGS
                     STATPSSGLCVSSNFVLNLSGNSIGSGQTYQWQESTTFGGPYTDLGSSSSSPLLNTTA
                     TTTKYYQCKVTCSGNDQYSTPVLVTVNPSFPGGTYTINSALPTSGSNFQTFNDFKSAL
                     NCGIAGAIVVNVVSGSGPYNEQIEFGEIANASSINTITINGNGNVLTFGATVSTAPGT
                     LVLNGTDYMTVNNLIVEGTGATYALVCHLWNNANNNAFNNCTFNAPANGTITTMVPFS
                     ISGSATSATTSGNSGSNNVITGCTMFSGYYNTVLVGNSGLPSTGNQVINCNINDQNLY
                     ATYCLYQDGVVLRGNTLSRPTRTSLGTYYGIYISTGCTNALVEKNRIRNTWATNTGST
                     GTQYSIYCTIDATLGNENKFFNNVISDIEFNGTIYGMYMSGSDYWQAYHNTISLDQTT
                     STATSTTYGVYATGTTAYDFRDNIVTISRGGTGTKYGVYYSTPAAATSDYNNIYMNAG
                     AGTNYVAYSGAPYTTLSAYQIASGKDANSSSVDPFYLAPGSPTYNYTPNNGLSNDTGT
                     PVGVTDDINGAPRSLTTPDIGAYEFSLGGLDAGITWFTPTSPTSPGMKSITVNIANTQ
                     AQTINSVQLSYSDGGAPVTQTFTALNLLSGNNTNLTFTTQYNLTSSVTMTVDILAVNG
                     GSDAVSGNNSDTYYLCVAPSGTYTINSALPTGGSNYQSFTDAIQALSCGITGPVVYNV
                     VAASGPYNEQITIPQIDGASTTNTI"
BASE COUNT         5009 a         2781 c         3093 g         5378 t
ORIGIN      
        1 agtatataaa aatttgaaat taagccttgc cggtgaatat caattaaaaa atgtggcaac
       61 ggttttgtca gcgatagaat tattgaaaga ttcatgtttt gtaattgaga aaaaaaatgt
      121 aaaaaaagca cttacagata ttcagcaatt gacaggattc agaggaagat ggcaggtgtt
      181 gggaactaaa cctctcatta tcgcagacac cggccacaac gttgacggca ttaaaacagt
      241 aattcgccaa atttgtaaga taagttttaa aaggcttcat atagtaattg gcatggttaa
      301 cgataaagac ataatgcctg tcttgaagct attgccaaaa catgctacct actatttttg
      361 taatgctaaa atccctcgtg caatgcctgc taaggagttg aaaaacaagg cgatagaagt
      421 ggggctcaat ggtcaaattt atacatctgt caaacaagca ttgttaagtg caaaaaaagc
      481 agcagtagca caagatttaa tttttgttgg tggaagcaca tttacagtgg cagaagtgct
      541 ctgattgtgt gtaactattc acccaaaaaa ctgcactttt tcagtgtagt tagggtaatt
      601 ttgtgacgct taaaaaatca agattacgat gaagtttaga tttacaattt tgttcatggc
      661 agtggtgttt ttagccaatg ccgatcaaac tatttttccg gttaattcag tatggaaata
      721 tttagacaat ggaacgaatc agggaacaac ctggcgcgac tcatcattca atgatgcagc
      781 atggagtagt ggtactgcaa aatttggtta tggcgaaggc gatgaaaata ctgtagtgtc
      841 ttacggacca aatccttcaa acaaatacat tacaacctat ttcagaaaag ctattagtat
      901 ttcaaacctt gcaacattct ctgcatttga gttaggtgtt agacgtgatg atggtgttgt
      961 agtgtatgta aatggtgttg aagcatggcg tgataacatc aatggtgttg tgtctgatac
     1021 ttcacttgct gctagtgatg cctctgatga tggtaattct ataattactg ccaccattcc
     1081 aacatcttac ttcgtaaatg gcaacaacgt tattgcagtt gaaatacacc agtttgttgt
     1141 gaacagttct gatttaagtt ttgatatgcg gcttacgggt cttgaaactc cgccaccatc
     1201 aaatgtgaca atcttccctt taaactcagt gtggaaatat ttggataatg gaaccgatca
     1261 gggtaccact tggcgtgatg tttcatttag cgatgctaca tggactactg gcccgggaga
     1321 atttggctat ggtgatggtg atgaagctac agttgtttca tacggaccta acccttcaaa
     1381 caagtatatc acaacatatt tcagaaaatc ggtttctatt gctgatgtta atgcatatac
     1441 ttcatttcag attggagtaa agcgcgatga tggtgttgtt ctctacgtta atggtgtaga
     1501 agtatggcgt gataatattg caggaactgt atcctacaat acattggcaa cagcagatgc
     1561 tgctgatgat gggagttctg tattgactgc taatattcct acatcatact tcgtaaatgg
     1621 caccaacgtt attgcagcag aagttcatca gtttgttgtg aacagttctg atttaagttt
     1681 cgatatgtca ttaacaggaa tgggaaatac accgccaaca atgccgtcaa tagtaatagg
     1741 ttcttatttg aatgtggcta cacctacaag tattattgtg aggtatagaa cagatattac
     1801 agcaatgtca aaagttgttt atggtacaac ttcaggagtt tttacggata gtacagttag
     1861 tgcatcgcct gtcacagaac atattgttca gcttagcaat ttacaaccaa acacaaaata
     1921 ttattatgct gtaaaatcag gaaataattt tattcagaat aatccggcag aaaatttctt
     1981 tatcacacca cctgttgccg gaacagctaa acccacaaga atatgggtga taggtgatat
     2041 gggatttgga agtgtagaac agaatcaggt tcgtgatgcc tattataact ataccggaag
     2101 cacatataca gatctttggt tgtggcttgg tgacaatgct tataattctg gaaccgatgc
     2161 agagtaccag aataatgtgt ttaacaacag atatgagaaa atgcttcgtc agacagtagt
     2221 ttggcctgca gcaggtaatc atgaaatgta tgcatcaaat gcagtcacag aaacggggcc
     2281 ttattatgag ttgtttaact tccctactaa cggagaagcc ggtggtgtac catcaggtaa
     2341 gaaagcatat tattcctata actatgcaaa tattcacttt gtagtacttg aatcaacaac
     2401 ggcatcattc agaactattg gcggtgctat gatgaattgg cttcagacag accttgctgc
     2461 caatacacaa aaatggacag tagtttattt tcatcaccct ccatattcaa tgggtagcca
     2521 caactctgat acagaaactg aattgataga aatgcgtcag aactttgttc ctattctcga
     2581 gcaatataaa gtagatttag tgctatgtgg tcacagtcac aattacgagc gttcgtttat
     2641 gattaaaaac cattttggat cggagaatac attcaacagt tcaaatttag ttgatggcgg
     2701 aagtggagca ggcgctacac catatattaa aacagcatcg tctaattttc agggaactgt
     2761 ctatgctgtt gcaggaacag gaggaattct tgaaggtatt caggcaagtt ggccacatag
     2821 tgccatgtat tcttattctt ctgcaacgta tggttcaatg gtattggata ttgatggaga
     2881 tactttgtat gctaaatttt tggataatac tttacctaat ccaacaatct atgatcagtt
     2941 tactattatc aaacaatgtg atcagcaagt gacactggca ccattcagta atgtatgttc
     3001 agggcaaagt tcttttgcac taacaggagg aagtcctgca ggaggaagtt acagtggtac
     3061 aggtgtaagc aatggcatgt ttaatcctgc agttgccggt ataggcacac atactattac
     3121 ttatacatat actaacggat tttgtagcga ttctgccaca tcaacaatta ctgttacaac
     3181 aggaggacag attgcaacaa ttaacccttc agggaatatt acattatgtc aaaacgactc
     3241 tgttctatta tctgcaaaca gtggaacagg cttaacttat caatggcaat tgaatggttc
     3301 agatatttca ggtgcaactt ctcaaactta tacagccaac ggtgcaggca actacactgt
     3361 agctatcact tcatcatgtg gaacagttgt ttcatcagct acaattgtta gtgcagcttc
     3421 ttcagttagt atttcgcctt caggatcagt aaacctttgc tcaggtccgg ttacactttc
     3481 tgtaaacaca ggtgtaggct ttaattatca atggaaaaat aatggtgttg atattaacgg
     3541 agctacaaat agtagcttag agatattaag tgcaggaaat tattctttat tagccactga
     3601 ctcaaccgga tgtgcgtcat tatctaatat cgtaaatgct actggtagtg cacatgtaat
     3661 ctctgcgctt ggctcaacaa ctatttgtgc cggaggaaat gttactcttt ctgctcaaac
     3721 gggatcaggt ttcacctatc aatggtatag aaataatggt gttatttcgg gtgcgattaa
     3781 ttcaacttat aaggcaacaa gccacggcaa ttattattgt cgtactacag cacccggttg
     3841 tacaggcaat tctaacatta ttgtagttgc agttattaat aaccctacac caactatttc
     3901 tgccaatggg tcattgaata tttgtagtgg aagttcagtt atactttcaa ccaatacttt
     3961 tcccggagta acctatcagt gggaaagaaa cggttctgct gtaaatggtg caacatcaca
     4021 attgcataca acaacacttg ccggaactta ccgtgtctca caaacagcaa atggttgttc
     4081 taaaaaatcg ccatcaattt ctgttaagat tgttgcagga ccgacagcta ctattaatgc
     4141 taacggaagt gttaatttat gtaatggaca aacagttaca ttaaatgcta acactgttac
     4201 cggtgcggca taccaatggc ttgcagatgg agtgaatatt gccggtgaaa ctaatcaaag
     4261 tttggttgta aatacatcag gaaattatca atgcagaatt accacaacat gtgctgcact
     4321 ctctaatgtt attactgtta cagcaagttc aatgcagatt tctatttcac cctcaactac
     4381 tcaaaatatt tgtcagggta atagtgtgca gttttcaact accaatgacc cgggtaacag
     4441 ctatcaatgg aatattgatg gtagtgcaat acccggagcc atttcagatt cttattctgc
     4501 taacgtatct ggcatttact cagtttccat tactaatggt tgcggaacgc aaacatcgca
     4561 agctgttact gttaatgtat tacctgtttc agcagtgata tcacctgatg cagcgcaggt
     4621 aatttgttca ggcggaagtg taaactttac tgcaaattca ggttttggaa atacttatca
     4681 atggaattta aatggaactc caataaatgg agcaacaaac caaactttta atgccaatgc
     4741 tgcaggaaat tattctgtta ctgtttcaaa tagttgtggt gtttatacat ctacttcggt
     4801 atcggtttct gttgggtcaa tctctgcaac aattagccct gatattgcac agactatttg
     4861 ctcaggcgat agtgttctgt tatcagcaaa tcctgcaagt ggcatatctt atcagtggta
     4921 tttagatggt aatgcaattg gtggagcaaa tacagcaact tattttgcaa aatcacaggg
     4981 aaattatacg gtaaatattg tgagttcatg cggaagttct acttcacctt ctgttgcact
     5041 tgctgtaatt tcactttcaa gtagtataac acctgcagct actcaaatta tttgtccggg
     5101 ttcttttgtg ctgttgtcag ccaacacggg taatggacta acttatcaat ggaaaaaaga
     5161 tggtgtatta attagcggag ctacacaatc aacattctta gctgacgaga ccggaatttt
     5221 tactgttgat gtaacaaata gctgtggcac gcttgagtca aatcaggtaa ctgtaaatgc
     5281 tgcactaatc aattcttcta ttactccatc aggaacttct actgtttgtt caggtactcc
     5341 gcaaacattt tctgtgttac caacagcagg agcaacttat caatggtata gaaataatgt
     5401 tgcaattaac aatgctacaa atgtttcttt aaatgcatca agctcaggcc aatattatgt
     5461 aaaagtgcat ttgtcaggtt gcaatattgt aacatcaaat acggcaatac tttctgttca
     5521 aacaaacacc acacctgtag taaccgtaac accgggaaca acattatgta atgggcaaca
     5581 ggcaacatta agcagtaata tttattccgg tgttgcaatt caatggcaac atgaaaaagt
     5641 gaatattcca ggagctacat cacaaactta tcaggcaagt gttggagggc aatacagagt
     5701 agttcatacg tttaatggtt gtgttagaaa ttctgctgag aaatcaatta aattcgttag
     5761 ctgccgttcc gaaggttatg aatctgattt ggaaatgcat gtttcagcag aggtattccc
     5821 gaatccattt aaggaagtaa caaatattaa gttaagcgga tttgatatct cctctacaac
     5881 cattacagtg accaatattc ttggtgaaat gattaatagg tttaatcctg attctgaaaa
     5941 tattgaatgg aaacgaaatg gaaatgcgcc aggtatttat ctgatacaaa ttacagatgg
     6001 atataatcat cgcatcacac aaaaaattgt aattgaataa ttggtgaatt gtgtaaaact
     6061 ttaactgaaa accgactttt tataagtcgg ttttttgttt cttagtagga gttgaaagtt
     6121 aagtgtttat tacaatttat ttgtcatgac tacaaaacaa tttttacttg catttttatt
     6181 ttgtttgcca cttttttcca aggcagatac taccttggtt agttttaatg atgcatggaa
     6241 atattttgat aatggatatt tgcctgctac aaactggaat cagattatgt ataatgattc
     6301 agcgtgggca attggaaatg gagaatttgg atatggcgat ggtgatgaaa ctacagttgt
     6361 aagttacggt ggtgatccaa ataataaatt tgtaacaact tatttcagaa agacaatcag
     6421 tttaaacaat ccgtatgatt ttgcctcata tcatcttgat ttaaaaagag acgatggtgc
     6481 aatagtttat ataaatggca cagaagtttt cagatctaat atgacctcag gaagtgtagg
     6541 tttcaataca cctgcttatt ctgattgtgt tgatgatgga atggcattac aatcaatagt
     6601 attagttccc gcagattttc ttgccggtgt aaatgttatt gctgttgaag tgcatcagat
     6661 aaataatgtc agttcagatt tatcgttcga tcttcaatta aatgcataca ataattttgt
     6721 gcctcaactg ctgcgtcaac cttatcttca gatggttgcc gattcagcaa ttacaattcg
     6781 ttggagaact aatgtttcat gtcaaactgc agttctttat ggcaatgatc cggattcttt
     6841 aaatttttct gtgagtgatt ctgcaaatgt aattgatcat gagataagat taactgggct
     6901 cagtcctgat acacgatatt tttatgccct tggtgatgga acaaatgttc ttcaaggtga
     6961 ttcaaataat tattttaaaa cagcacctgt aaaaggaatg ccaaagcctg tcaggatttg
     7021 gtcaatgggc gatacaggaa ttaacaccat tgatcagaat aatgttcgcg atgcatttct
     7081 gaattataat gactctttgc caatagattt gttgtatatg cttggtgata atgcctatta
     7141 ttcaggcaca gatcaggagt atcaggatgc attttttcag aatcactatg aagatatttt
     7201 aaaaaatata cctgttttta gtgttgccgg caatcacgaa aattatacag caaatgcaat
     7261 cacacaaaca ggtccttatt atgatatttt tacttttcca aaaaatggag aatgtggtgg
     7321 tgtgccttca ggttcagaag cgtattactc ttttgattac agcaatattc atttcatagc
     7381 attagagact aatacacttt cgctgttgca gcctggaagt gatatgctca actggttgca
     7441 aagtgacatt gctgcaacac aacagaaatg gaaaatagtt gtatttcata actcacctta
     7501 caccaagggt ggtcataact cagatactga gacagactta attctgatca gacaaaatat
     7561 tatgcctatt ctggatcaac ataaagtaga tttggtatta tgtgggcata accatgtgta
     7621 tgaacgttct tattttatgg atggacacta tggtttatcg tcaacatttg attcaacaat
     7681 ggttgtagat tcatcttttg gtgaactacc ccaaccatat ctaaaaaagt cgtcaaagaa
     7741 ttataaagga ttggttgtag tgcaagccgg atgctccggt gttttagatc ctattggccc
     7801 gcaatggcct catcctgcaa tgaaaagata ttatgatcag gaacttggtt cattacttat
     7861 cgaaatagaa actgatacac taactgtgac attcgttgac aataatgtta gttcaccgca
     7921 ggtgctggac gaatttagtg ttgttaaaga atgtaatgtt gttccacaac ttacagttcc
     7981 tgttgcaaaa gtttgtgtta ctgatgatcc agtgcaattg agtgcaacac ctattggcgg
     8041 atttttttca ggcctcggtg tggttgatac agttttcaat ccaactgttg ccgggcaagg
     8101 gatacataca attgcctata cctataatga tatttatggc tgtcatgtta ccgatacaaa
     8161 aactattgaa gtagtaggag gagttccttc acaacctttc ggtattgttg gttctatttt
     8221 ggtttgccct ccttcaaata atgaagtgtt gtcaattcaa tcattgcctg atgcggatag
     8281 ttacatctgg tcagcttctg ccggagtaaa tcttacttct tcaaatacag ataccttgat
     8341 taagtttgat ataaatacag ttcttccaca gtacaacatt tctgtcagtg ctttaaatat
     8401 ttgcggaagc agtattcctt ctttagtcaa tattacttca gttaaatctt ttccggttag
     8461 tttaacagga aatagtgtta tctgcccggg tgatacttct gtttatacag taaatgtttt
     8521 caatagcctt tcatcatatt ggtggcaacc ttctgaacac gtttttttaa acggacaatc
     8581 cgatataatc aatacattta atacattgca tgtgaatgct tctgttgaca gtagttttac
     8641 cgagggaaaa atttgtgttg caagtaaaga agcatgtcta aggaataatg ctgttagttg
     8701 tattgattta agtacaaagc cacctgttcc cggtgttatt tcaggtccgg ctgctgtttt
     8761 gggtggaagt aatggtgttg tttacagcat tgtgccggtt agtggtgcaa caggttattt
     8821 ctggacggtg acaggtgatg ctatacttac tgcagggcag ttcggcaatt ccgttaccgt
     8881 tgacttcctc ccttcgttta cagggggata tgttggtgtt aaaagccaaa gtaactgtga
     8941 tgaaagtatt gaggtttcta aacgaattaa aacctatatt attcaggttt cacagaatac
     9001 agtttcgcag acaagaatag caaatgaata caatcaggat aaaattgttt ctgtgtatcc
     9061 caatccaatc ggcaatgaaa tgcatgttaa gttgttgttg caagatgcct catcaaatgt
     9121 tcttgttgtt ttaaaggatg ttgcaggaaa agaagttgga aaatggagta aaaaggtttt
     9181 ttctgatgag gacgatatta ctttgaatgt ttcaggttta agcagtggtg tttattttct
     9241 atgtgttact actgacaata tgcagtggaa tggagtagtg gttaaacaaa atgatcattg
     9301 ataagttaag ggtaggctga ttgtaacttt tgttcctaag cctaatttgc tttgtacttc
     9361 aatttttcca ttaagaattt gaacaagttc agaaataata atataaccta ggccaaatcc
     9421 ttctttgttg tctaattgtt catcttcctc atttaaaggt tgcttattta taagcttagt
     9481 tattcgggtc aactgttgtt cagacatacc tttgccgtta tcctcaatgg taatttcata
     9541 ttgattgttt tgtttggaat aaatttttat aaaaccattt tcagtaaatt ttgtggcatt
     9601 ggaaattatg ttatgcaaaa ttattgatag taagtttgag tcggattgaa tgatgtcgtc
     9661 atgagatatg gtgctgatga gcctggtgcc tttgttgtat gttaagtgca taaattgctc
     9721 tgtaatatct tctgccaatg ctcctatagc aacacttgaa atctgttttt ttattcgttt
     9781 gttttgatgt ttaatccagt tcaaaatgtt ttcagcattt gcctgaagct tttctgtagt
     9841 gttttctatt tcacctaaca attctttatg ttttttattg tccatttcta tctcttcact
     9901 cgcccccttt tttgcaacca ttgtaataaa tcttaaaggt gttatcaaat catgtgaaat
     9961 aatagaaatt agtttgtctt ttattttgat agattctgat aattcattat ttgctgtttg
    10021 tagttctgaa gttcgtaata caacattttt ttcaagtagt atattttttc gccttatgtt
    10081 aaaggcataa attcttgcaa caataattat tattaaaaga gcaagtccta ccatcgctaa
    10141 ccaaaaccat attgtttcgt aatatttatt ttcaatatga aatgcaatag ttgcttctgt
    10201 ataatcttta gaaagtcctg tttgtttgcg tattgtaaat ttgtattctc ctgatttaag
    10261 gttagaaaat tctaaagcag attcgtttgg ggataagtaa atccattgct tattgtatcc
    10321 ttctaatttg tattcacaga ataaattttc aggttctccc cagtatgggt tagaaaattc
    10381 tattctaaca tattgaacag aatttggaac ttttatatta tttgaagtat aataaataga
    10441 atcatctgcg ataaattggt caatgcttat tggataatct gaccttgggt cttcaatttt
    10501 ttcaggataa aaccaaacca atccttctac atttggtagt gaaacatagc cattgttaag
    10561 tcttaaataa ttagggtcac atccaccatt aaattcaggg ttactgatgc catcttcttc
    10621 tgaataatga aaataggaaa cctgataagt tgtatcgtta aaatagtgga caagttcagc
    10681 aaattttgtt ttgataactc cattgttggt agccatccaa agaaacccca acgaatcagg
    10741 cacaaaatta tgtacctgtc ttaaagcttc aaatctgcca tttggcattt ttacagtctt
    10801 accgtttttt atacatgtaa tagccacgcc catatgttcc aatcagaaga taattgttaa
    10861 tagctttaaa atgataagtg tatttcaagg caagttcggg caaggtgtct aacttgccac
    10921 tttgagtgtc atatttataa ataccggtat agttacatat ccaaagttta ccttcaaatc
    10981 gtaatagctg aaaaatatta gaatttgatt catcattgtc taatattttg atatgtttta
    11041 atgtatcatt tacaacacag ccgaaacttt tgatgagccc aacccacata gtgtcgccct
    11101 cttcaaaaaa agaaagcgga agttcgtccc ctgtatagta gatgtatttt tttgctttgg
    11161 tttttggatt aaaacgaacc aatgagttat tctcagcata ccaatagttg ccttttttat
    11221 caatacaaat gttttcggaa taatttctag caaggttgag cttactttgt ttgcctccat
    11281 taatggtaaa ttctctgttc caatcggtat aaacagtgct gtcatttaag gctatttgac
    11341 aaaaataagt gttgtttgtg ccgggttcgg gattggcgaa ctctaaggat ttgaaattac
    11401 gtttcttgta aacaaataag ccgcgtgtat ctgtccctat cattatcaga ttctgatctt
    11461 cattataaat gattgaggaa ataagacagt tgtttgggag ttggtttgtc agttgaaccg
    11521 tgctaatcaa atttgcattc aatgagtccg gaaaaaaaga atacaaagta taatctacct
    11581 gaacacaagg gtcactattg ttgaaatccc agaatgtgtt agtgatttta cgggagtaat
    11641 ttttacagga acttaaatct ccttttaatt tacatttaac caatgaccac gatgttaaat
    11701 caactctata tatttcagaa tcatttgcga aaacataaat atttccatta gcagaaatga
    11761 acttgcggtt ctgtaaatga tctaaattaa gctccttaag tttattcttg cctttaaagt
    11821 gtgcaattcc tttttttgtc ctgacaatgt atgaattatc cggtaaggga aacatgatta
    11881 acggagttaa aatccaactt ttgtcaacgg ttatgtttac atgaggtgtg gataattcca
    11941 gatatgtttt tatgtctgga agtgttcctt tgatatttat ataattaagt gagttgattt
    12001 ttccttttct taatgtgtca cttgttaaac ctttaatctg aaagatattt ccacttggtt
    12061 ctatcattat cttgtcggca ttggtggttt ggtagaaata caagccaagg tagggcgcat
    12121 ctgatgtaaa atgctttttg aaattaatgc cgtcaaaacg tgctaagcca ctgccataca
    12181 caacccaaac atatccgttc ttttcataaa acaaccccaa tacattatta tcaggaagcc
    12241 cttttttaat agtgtaattt tggatactgt aactgttttg tgctgataat ttaaaaaatg
    12301 taaaaagaaa gacaagaaaa agaactttta atttatgcat ataccttaga caataggaat
    12361 gttataggct cgtgctgagt tttgtaaatc cataagatta ttgacaccaa tcttatcaaa
    12421 aattcttgcc ttaaaggttg ctactgtatt ggattttaaa tcgaagtgtg atgctatttc
    12481 tttaacgctt ttacctttta gcaatagcag taagacttct gtttcgcgtt ctgacaaatt
    12541 tgaaaacggg ttttcacttt tacctttttg tacaccgtgc tgctgatcaa agtatttttg
    12601 acttacatat tttctgcctg taaagaatag ctccatggct tttactactt cttcttcgct
    12661 gctttgtttg ttaagataac ctgatgcacc aagattattt aaccgtttgg caaaaatttc
    12721 ttccggactc attgagtaaa tcaggattgg caacagagga aattgttgtt ttatctcctg
    12781 aaaaatttca agtaaattcc catcagacaa ttgcatatct aaaatcagat gtgtgaaatt
    12841 atttttttca agtgaatcaa gaattccttt tgaagaatct gtttctacaa tattgaattt
    12901 gccaaaatgg gtttcaagta aaaattttaa accgcgtctg atgataacat ggtcatctgc
    12961 aatgaggagt gatatatctg aaaatgcatt cataatttta gtgagtttta tcggataaaa
    13021 gtactcaatt tttaaattgt agtgtaatgt ttacaattag tattttttta ggttcttatt
    13081 ttaaaaaaca atgttaatta ttaagtactt acgaagatgt tggaagtgcg tagagcaacc
    13141 cctacaaatt aactcaaatt gtttatttta atttgttttt tgaaagaagg gcagaacttt
    13201 aaaccaagta ttaagagtga ttaaaaccaa aacattcgca ttttctcgta tcaatcttgc
    13261 ttcgggcata caattgcttg atggctggtt tttaattaat cttaactata ttgttctgaa
    13321 ttagtaaagt aaaaacaaaa aatataaata actaaatatc aaacaatgac tactttaaat
    13381 taccaaaaca aaaatccctt agtacaattg cttagggtag cggtgtgttt tttaataaca
    13441 ttattgctaa attttaattt gcaggcacaa acaactgtaa ccattggtgc agggtctcaa
    13501 agtggtacac agtccaatag tgccgcaggc gattgtggcc ctatgtatcg ttcaactgcc
    13561 agtagtgcat ttgtgtattc aagacatcac tatttataca cgcagtctga gatttcttct
    13621 gcaggcattc catctggtaa tttgattacc aaattggcat ggtacaatca aaatggtgcc
    13681 ggaacaaatt ctccatgtat ttttcaaata tggatgaaaa actcagcctt aaccgatgtt
    13741 ggagtcggtg gtcaaaattg gtcaacttta acttctggct caactttggt ttacaacagc
    13801 agcacacatt cagttcctgc cacagttggt tggcacgata ttgttttaag tacacctttt
    13861 acatatacag gtggtgctat agagatttct gtaaattttg atatatctgg tggttcaagt
    13921 ccttatacaa caaatggttt ctcatggaac aaagatccaa tcacagggcg tactttatct
    13981 tatgtgggta gtgcagcacc cggagcaaca ttacctaatc ttcgtactgt aagagcacaa
    14041 attcaaatga cttatcaaag tggtgcacca tgtagtgctc cacctacagc tggatcatct
    14101 acagcaacac cttcatcggg gctttgtgtg agttcaaatt ttgtgttaaa tttatctgga
    14161 aatagtattg gttcgggtca gacatatcag tggcaggaat caacaacctt tggcggccct
    14221 tataccgatt taggttcttc ttcaagttcg cctttactta atacaacagc aacaacaact
    14281 aaatactatc aatgcaaggt aacctgtagc ggtaatgatc agtatagcac acctgttttg
    14341 gttactgtta atcccagctt tcctggaggc acttacacca ttaattcagc tttgccaaca
    14401 agcgggtcta acttccaaac ttttaatgat tttaagagtg cgttaaattg tggtattgct
    14461 ggtgctattg ttgtgaatgt tgtttccggt agtggacctt ataatgaaca gatagaattt
    14521 ggtgaaatag caaatgcatc ttcaataaat acaattacta ttaacggaaa tggaaacgtt
    14581 ttaacttttg gagcaactgt aagtactgca ccgggaacat tggtgttgaa cggcacagat
    14641 tatatgacag ttaataactt aattgttgaa ggtaccgggg caacctatgc cttggtttgc
    14701 cacttatgga ataatgcaaa taataatgcc tttaataatt gtacttttaa tgcacctgca
    14761 aatggaacta tcacaacaat ggtgcctttc tcaattagtg gctcagctac ctctgccaca
    14821 acttcaggta atagcggtag taataatgtg ataactggtt gtaccatgtt tagtggttat
    14881 tataatactg tacttgttgg taacagtggg ttgccaagta caggtaatca ggtgatcaat
    14941 tgcaatatca atgaccaaaa tttatatgct acgtattgtt tatatcagga tggagttgtt
    15001 ttgagaggca atacactgtc tcgaccaacc cgtacttcgt tgggtactta ttatggtatt
    15061 tatatctcaa ccggatgtac caatgctttg gtagaaaaaa atcgtattcg taatacttgg
    15121 gcaacaaata caggttccac aggaactcag tattctattt attgtactat tgatgcaaca
    15181 ttaggtaatg aaaacaaatt ttttaataat gtaatttctg atatagaatt taatggaaca
    15241 atatatggta tgtatatgtc aggtagcgat tattggcagg cttatcataa tactatttca
    15301 ttagatcaaa ctacctcaac agctacaagt acaacttatg gagtgtatgc aacgggtaca
    15361 actgcatatg attttagaga taatattgta acaataagca gaggtggtac cggtacaaaa
    15421 tatggcgttt attacagtac tcctgcggca gctactagtg attataataa catttatatg
    15481 aatgcaggtg ccggcactaa ctatgtagct tatagtggcg ctccatatac cacattgtcg
    15541 gcttaccaga tagcctctgg aaaagatgcc aatagttcaa gtgtagatcc attctatctt
    15601 gctcccggct caccaactta taattatacc ccaaataacg gtttgtctaa tgacacaggt
    15661 acacctgttg gggtaactga tgatattaac ggtgccccac gttctcttac tactcctgat
    15721 ataggtgcct atgaattttc tcttggtgga cttgatgcag gcatcacatg gtttacacca
    15781 acaagtccaa caagccccgg aatgaagtca attactgtta acattgcaaa tacacaggca
    15841 cagactataa acagcgttca gctttcttat agtgatggag gcgctccagt tacacaaaca
    15901 tttactgctt taaatctcct ttcaggtaac aatacaaatc tgacatttac tactcagtat
    15961 aacttaactt caagtgttac aatgacagta gatattcttg cagtaaatgg tgggtcagat
    16021 gcagtttcag gaaataacag cgatacctat tatttatgtg ttgcaccatc cggcacctac
    16081 acaattaata gtgcattacc tacaggcgga agtaactatc agagttttac ggatgcaatt
    16141 caggcattaa gttgtggtat cacaggtccg gttgtttata atgtcgttgc agcttcaggg
    16201 ccgtataatg agcaaattac aatccctcaa attgatggcg cttcaacaac gaatacaata
    16261 a
//
LOCUS       DAOOOL010000050        16257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_296_length_16257_cov_89.129058, whole genome shotgun sequence.
ACCESSION   DAOOOL010000050 DAOOOL010000000
VERSION     DAOOOL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_16257_cov_89.129058"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="PKU82_05750"
     CDS             <1..1907
                     /locus_tag="PKU82_05750"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOZ82413.1"
                     /translation="NTLQAKGVLDSLKNKIEAATTLNELEDIYLPFKEKRKNRAQVAR
                     DAGLEPLAQTLIHDDRKAKQLAEKNISSTFSDTEKVLQGARDIIAELISENHQVRKTF
                     RQCFEQKSFIVSKVIKSKVEEAVKYKDYFDYKEKISSCAAHRLLAILRGEKEKLLRVS
                     VMPDSTFLLNLLFDTIKTEGFYLNTQVKTAAEDACERLLVPSMENETIGHYRQKAETE
                     SARLFAVVLKQLLLAPPLGQKNILAIDPGFKSGCKIVCLDKNGRLLHNETIFPHPPQS
                     QQKIASHKISTLVSQYKIDAIAIGDGTAGRETATFIQRLRFDRKIQVFVVNEDGASVY
                     SASAEGRKEFPEYDVTVRGAVSIGRRLMDPLAELIKIDPRSLGIGQYQHEIDEMMLNT
                     ALDQTIEHCVNTVGVNLETASEKLLTYVSGIGEVMAKKIIDARANSGIHSRNELKNIK
                     GMGDKTYEMCAGFIRVNGDNPLDKSAVHPENYTLVERMAKDIDTTIAGLIGNDSLIQK
                     IDISKYVSAEVGLPTLKDIIAELSKPGRDVRGIIKVFEFNTEIKSINDLQPDLLLPGI
                     ITNITNFGAFVDIGIKKDGLVHISEISDEFISDISSAVRLNQQVMVKVLSADSKTGRI
                     QFSMKGIQQP"
     gene            1934..2320
                     /locus_tag="PKU82_05755"
     CDS             1934..2320
                     /locus_tag="PKU82_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOZ82414.1"
                     /translation="MNIQPEKVRIDKWMWAVRIFKTRSQAAEACAKGKVFIDETAVKA
                     SRMVKTGETISIRRGSFTLVFQVIQPIENRVAAAVKDQFCKDLTSADEYEKIKMHSLA
                     VRTYRQHNEGRPTKKERRALDDFLDW"
     gene            complement(2327..4618)
                     /locus_tag="PKU82_05760"
     CDS             complement(2327..4618)
                     /locus_tag="PKU82_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOZ82415.1"
                     /translation="MRKRQYGILITLILLVASCSPFKQLDKGEYLLNRNQVIIDKPEL
                     REGVKAIIKQKANRRILGLFRFHLAVYSMGNRGKPTKFKNWLKNTVGEEPEVLDTLFT
                     KKSTQQIKQFMSKNGYFDAEVKDSTIYRKKKAIVRYTIVAHKPSFIKYVARECADDNL
                     DSLVIKDTSTSKLQAGIIYNEGILQGDRERLTKKLRNEGYFQFSPQFVSYRVYTSDTT
                     KNIAVFQDVAAFSNPDSTISSAALPHTKFKLNRIIFSPDYDPLSLNTSLIQYDTVAYK
                     GYLFVKRAGKRMPVKPEVIVNHTFLKPEAVFRQSNADYTYKGLNLLGMYRFVNINYNV
                     VDSNRLDCFINLGTTPKQEYKTELEGTNNGGNLGVAGDFTYRNKNVFRGAEMFEFKIR
                     GALESQKKFTDDKENKVLLLFNTYELGVENSIYLPKAIKPFNRLFKNIAANPVTVFST
                     NYNIQNRPEFNRSILDFSASLEWRKGRFIKQIVTPFQVNFVNVKLEPEFKARLESLND
                     PVLFSSYDNHLISNGRYTLIYNNQQLNEPGNFKFFRTNIEFAGNTLYWFRQIEGTDKD
                     ANNSYTILNKRFAQYFRPDADFRYYQNINAHNSIVYRIAAGIGIAYGNSKIIPFEKSF
                     YAGGSNDLRAFRARNVGPGNFTSVNNFERTGDIKINGNIEYRFDILKILKGAFFLDAG
                     NIWVRKKESSRPNAEFEIDKFYKQLAIGSGLGFRFDFTFFIFRLDIGVPIVDPQYKGD
                     DKMVADEVKPRSLIYNFGIGYPF"
     gene            4690..5454
                     /locus_tag="PKU82_05765"
     CDS             4690..5454
                     /locus_tag="PKU82_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOZ82416.1"
                     /translation="MLTSSEIKQIKSLKDKASRHEQQKFVVEGEKMVTELAHSAFNII
                     AVYATDEWIGQANNEILSLLNEKLIQVKTKELERISSLKQPNGVLALVAMRQQQETEI
                     KFNDIILAFDDISDPGNLGSIIRIADWFGIKQIVCSSTSVDLYNSKTIQATMGSIFRV
                     NVFYTDVEKWISANKQNATFYATMLNGKSVYSVAKKKPAVLVFGNESRGLSHSIQQLI
                     DLDISIPKFPDEKSSSVESLNIAMSAAIFCSEWRRD"
     gene            complement(5451..6161)
                     /locus_tag="PKU82_05770"
     CDS             complement(5451..6161)
                     /locus_tag="PKU82_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOZ82417.1"
                     /translation="MTASEEERIRKAFSDKDWQEIKAQDTWQIFKIMSEFVEGFEKMS
                     RIGPCVSIFGSARTKPDTRYYLLAEEIAYQLTQSGYGVITGGGPGIMEAGNKGAQRAG
                     GKSVGLNIKLPFEQTSNPFVDHDKNLMFDYFFVRKVMFTKYAQGFVVLPGGFGTLDEF
                     FEALTLIQTKKIGRFPIVLVGKEYWGKLFEWLQTVMVDEGNILPEDLSLFKLVDEAHE
                     AKSHIDAFYSKYLLKPNF"
     gene            complement(6270..7010)
                     /locus_tag="PKU82_05775"
     CDS             complement(6270..7010)
                     /locus_tag="PKU82_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOZ82418.1"
                     /translation="MNITLKVWKQRNKDERGRFETYQVQDVSEDMSFLEMFDVLNERL
                     INEGKDPVAFDHDCREGICGACSMYINGRPHGPLQGVTTCQLHMRSFKDGDTIVVEPW
                     RSAAFPVIKDLAVDRSSFDRIMAAGGFVSVNTGNAQDANSILIPKDDADKAFEAAACI
                     GCGACVAACKNSSAMLFVSAKVSQLSLLPQGKAERHERVMKMVAQMDDEGFGNCTNTG
                     ACEAECPKEISIENIARMNREYLSAKIS"
     gene            complement(7044..9053)
                     /locus_tag="PKU82_05780"
     CDS             complement(7044..9053)
                     /locus_tag="PKU82_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOZ82419.1"
                     /translation="MTNNILDAHIPAGEIEKKWSKYRSTVPLINPANKRNLEIIVIGS
                     GLAGASAAASLAELGYKVKVFCFQDSARRAHSIAAQGGINAAKNYQNDGDSIYRLFYD
                     TVKGGDYRSREANVYRLAEVSGNIIDQCVAQGVPFAREYGGLLSNRSFGGVQVQRTFY
                     AAGQTGQQLLLGAYSALMRQVGMGAITMYSRHEMLDIVKIDGKCRGIIARNLITGELE
                     RHFGHAVLMCSGGYGNVFYLSTNAMGSNVTAAWKAHKNGAYFANPCFTQIHPTCIPVS
                     GEHQSKLTLMSESLRNDGRIWVPKKKEDAEAIRAGKLNPNDLKEEDRDYYLERRYPAY
                     GNLVPRDVASRAAKERCDAGFGVNATGEAVYLDFAYNMKYKYGKMEATMNSIENPTEE
                     QLFELGKAVVKEKYGNLFDMYKQITGVDPYHSPMMIYPAVHYTMGGLWVDYNLMTTVP
                     GLYALGEANFSDHGANRLGASALMQGLADGYFVIPYTIGAYLSSEIRTKAISTDHEEF
                     VAAENRVRETLQKLMQIKGTKSVDHFHRQLGKIMWEKCGMARNAVALKEAIAEIQELR
                     KQFWADVRIPGEMNEFNPELEKAGRVADFLELGELMCMDALQRNESCGGHFREEHQTE
                     EGEAMRDDDNFAFAAAWELTGDSQWKMNKEELKFENIKIAQRSYK"
     gene            complement(9069..9857)
                     /locus_tag="PKU82_05785"
     CDS             complement(9069..9857)
                     /locus_tag="PKU82_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOZ82420.1"
                     /translation="MSSNSVLSLFKSSIGKKVIMALTGLFLSIFLVVHVSGNFQLFKT
                     DSGLSFNQYSVLMTTNPFIKTISYLLYITIVLHAIRGIMLVLENKKARPVSYAKYDGK
                     ANSKWASRNMGLLGTVILFFIVIHLNDFWYQYKFGHIPFTMYKTELTTGKLVEVKEME
                     AGFKMGSKMEEMIVGDHKYVIVKDLYKEVKDEFKEWWMVLIYVVSMAAISFHLYHGFK
                     SSFQSLGLNHRKYNGLIQSIGTWVFAIIIPASFAAMPLYFFINR"
     gene            10065..10190
                     /locus_tag="PKU82_05790"
     CDS             10065..10190
                     /locus_tag="PKU82_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82421.1"
                     /translation="MGHHHVEEKGQTFLYEGGTGAFTAVWMILVFAFIFFLIYLG"
     gene            10413..11258
                     /locus_tag="PKU82_05795"
     CDS             10413..11258
                     /locus_tag="PKU82_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOZ82422.1"
                     /translation="MPTTEQLIKISTQVRRDIVRMVHAHQSGHPGGSLGCADFLTALY
                     FEIMKHNPAGFSMDGKNEDIFFLSNGHISPVWYSVLARSGYFDIKELSTFRSINSRLQ
                     GHPTTHEGLPGVRMSSGSLGQGLSVAIGAAQSKKLNNDSSVVYSLHGDGELQEGQIWE
                     AMMYAAAKKIDNLIATVDYNGQQIDGAVNDVLSLGNLKAKWEAFGWDVLQMNGNDMKQ
                     VVETLQNARQHTGKGKPVVILMTTVMGYGVDFMMGSHKWHGIAPNDEQLKQALAQLPE
                     TIGDY"
     gene            11263..12663
                     /locus_tag="PKU82_05800"
     CDS             11263..12663
                     /locus_tag="PKU82_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ82423.1"
                     /translation="MSVIKSFMQHFRKLFFLAAFFIAFTSTVRAQKNNSLPLTRIEFL
                     FDASQSMYAQWQTNTRFEIAKKLLSEMVDSLDKITNVEMALRVYGHTKKFPPQDCDDT
                     RLEVPFGKNTAYLIKKRLSEISPSGTTPIAQSLDQCGNDFPTDPARNIIILITDGIEE
                     CNGDPCAVSLMLQRKGIILKPFVIGLGLSKEYKDVFNCVGNYYDAVDETGFRTVFNVV
                     ISQALNNTTIQVNLLDEKHRPTETNVNMTFYDTYSGALRYNFVHTLNAKGLPDTLLID
                     PLGTYDIVVNTIPPVRKDSNKIVAGKHNTIGIDCPQGDLFLKFDGTSDYKNLQCIVRK
                     KGDSKTLHVQAFNTTTRYLTGDYDLEVLCLPRMIINDVNIAQSKTTTVQIPNPGMATF
                     QTVSAGYGSVYQEKDNKLNWVCNLSENNMHESILLQPGNYRVVFRPKISKEAIYTIEK
                     TFRITSGSGITVNINK"
     gene            12671..13639
                     /locus_tag="PKU82_05805"
     CDS             12671..13639
                     /locus_tag="PKU82_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ82424.1"
                     /translation="MKKFTYTEKKDTRSGFGAGLLEAGKANPNVVALCADLTGSLKMD
                     AFEKAFPERFFQCGIAEANMMGIAAGMTIGGKIPFTGTFANFSTGRVYDQIRQSIAYS
                     GKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVINPCDYNQTKAATMAIAKH
                     FGPVYLRFGRPSWPIFIPENTPFEIGKALLLSEGSDVSIFATGHLVWKAIEAGMALAD
                     MGISAEIINIHTIKPLDEKAVIQSVSKTGCAITAEEHQINGGLGDSICQLLSRHLPSP
                     VEMVGVKDTFGESGTPDALMTKYGLDSKNIVFAVEQVLQRKKELAG"
     gene            13704..14249
                     /locus_tag="PKU82_05810"
     CDS             13704..14249
                     /locus_tag="PKU82_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ82425.1"
                     /translation="MNHYSDKELLEQFSNGNADYAFNLIVRKYQQKIYTHVRRLVADH
                     DDADDLTQTTFIKAWKALGNFRADAQLYTWLYRIATNECYTFLKNKRKRFFIPIHDVE
                     AELADKLEHEPSLKANELELKLQKAILSLPEKQRAVFNLRYYDEMKYEDMSEVLGTSV
                     GALKASYHHAVQKIEKFLQSF"
     gene            14280..14714
                     /locus_tag="PKU82_05815"
     CDS             14280..14714
                     /locus_tag="PKU82_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82426.1"
                     /translation="MKDEIYNELKDKAPLLSSIEKKHLFDTPENYFDQLPSAIQQKIK
                     QPQTSTIKTLFSRLLKPQFAIGLSVILLVGGFYLFNQHKADNQYAFVAVNVDSLNVNE
                     LSDLLDTDQLESIAPADNNADAETINYLIENYNDLSYDINDI"
     gene            14716..15177
                     /locus_tag="PKU82_05820"
     CDS             14716..15177
                     /locus_tag="PKU82_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82427.1"
                     /translation="MMKSAGIAVFILLFAGMAMAQPGQKKMDRRDKAEAMKIGFITNR
                     LSLTQDEAKKFWPVYNQFQDELSSLRQKRRGDRREAKQDFDQLSDKEVEKVVDDEIVF
                     RQSELDIIKKYHAQFKQVLPIKKVARLYQAEEDFKKELLRQLKDRRDARGN"
     gene            15456..15842
                     /gene="apaG"
                     /locus_tag="PKU82_05825"
     CDS             15456..15842
                     /gene="apaG"
                     /locus_tag="PKU82_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOZ82428.1"
                     /translation="MSTEVTAGIKVTVETFFQPTHSNPVQHHYVFAYRITISNESEES
                     IKLKRRHWYIIDSDNARREVEGEGVVGEQPVIEPGQLYRYVSGCNLNTEIGKMYGTYL
                     MERLSDNKMFYVKIPEFRMVVPSKMN"
     gene            15933..>16257
                     /locus_tag="PKU82_05830"
     CDS             15933..>16257
                     /locus_tag="PKU82_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOZ82429.1"
                     /translation="MHLIAPSILSADFSQLSKDVEMINNSVADWFHVDVMDGVFVPNI
                     SFGFPVIKAIKKHAKKPLDVHLMIIKPERYVEQFREAGADVLTVHIEASVHLHRTIQQ
                     IKKEGM"
BASE COUNT         5249 a         3101 c         2971 g         4936 t
ORIGIN      
        1 ttaatacact tcaggctaaa ggagttttag attcattaaa aaataaaatt gaagccgcca
       61 caacattaaa tgagcttgaa gatatttatc ttccctttaa agaaaaaaga aaaaatcgtg
      121 cgcaagttgc cagagatgct ggacttgaac cattagcaca aacgctcatt catgatgaca
      181 gaaaagcaaa gcaacttgca gagaaaaata tatcatcaac cttttctgac acggagaaag
      241 tattgcaagg ggctcgtgat attattgcag aattaatttc agaaaatcat caggtaagga
      301 aaacgtttcg tcaatgcttt gagcagaaat cttttattgt atcaaaggtc atcaaatcta
      361 aggttgaaga agctgttaaa tacaaagact atttcgatta caaggaaaaa ataagtagtt
      421 gtgctgcaca tcgcttattg gccattttaa ggggcgaaaa agaaaagtta ctaagagttt
      481 ctgtaatgcc ggattcaaca tttttactga atctgctatt tgataccata aaaaccgaag
      541 gtttttattt aaacactcag gttaaaacag ctgccgaaga tgcatgtgaa agacttcttg
      601 taccttcaat ggaaaatgaa accattgggc attacaggca gaaagctgaa acagaatcag
      661 caagattgtt tgctgttgtt ttaaaacaat tgctacttgc acctccatta ggtcaaaaaa
      721 atattttagc cattgacccc ggatttaaaa gtggatgtaa gattgtttgc ctcgacaaaa
      781 acggcaggct tttacataat gaaaccattt ttcctcaccc accgcaaagc caacagaaaa
      841 ttgcatcgca taaaatctca acgttggtaa gccaatataa aattgatgcc attgctattg
      901 gtgacggcac tgccggtcgt gagacggcaa cattcattca gcgtttgcga tttgacagaa
      961 aaattcaggt atttgttgtg aatgaagacg gggcttctgt ttactctgct tcagcagaag
     1021 ggcgcaaaga gttccctgaa tatgatgtaa ccgtccgtgg tgctgtttct attggtcgca
     1081 ggttgatgga tccgcttgcc gaactcataa aaatagatcc acgctcactc ggcattggac
     1141 aatatcagca tgaaattgat gagatgatgc tcaacactgc attagatcaa accattgaac
     1201 actgcgtaaa cactgtaggc gttaatctgg aaacagcaag tgaaaaactg ctgacctacg
     1261 tttcaggcat tggcgaagta atggctaaga aaattataga tgcaagagcc aactcaggta
     1321 ttcattccag aaatgaattg aaaaatatta aaggcatggg tgataaaacc tatgaaatgt
     1381 gtgcaggatt tatccgggtt aacggtgata atccattaga caaaagtgct gttcacccag
     1441 aaaactacac tcttgttgaa cgtatggcaa aagacattga cacaacaata gccggtttaa
     1501 ttggcaatga tagtctgatt cagaagattg atatttcaaa atatgtatct gctgaagttg
     1561 ggttacctac tttgaaagat atcattgctg aattatcaaa acccggaagg gatgtaagag
     1621 gtattataaa agtttttgag tttaatactg aaataaaaag catcaatgac ttgcagcctg
     1681 acttgttgct gcccggaatt attactaaca ttacgaactt tggagcattt gtagatatag
     1741 gaattaaaaa agacggactg gttcacatat cagaaatcag tgatgaattt atttccgata
     1801 tttcatcggc agtgcggcta aatcaacagg tgatggtaaa agtattaagt gcagatagta
     1861 aaacaggaag aattcagttt tcgatgaaag gtattcagca accctaagtg ctttaatgat
     1921 gataattttg aacatgaata ttcaaccgga aaaagtgcgc attgataaat ggatgtgggc
     1981 agttcgtata ttcaaaacac gttcgcaagc tgcggaggcc tgtgcaaaag ggaaagtttt
     2041 tattgacgaa actgctgtta aagcatcacg aatggtaaaa acaggagaaa caatttctat
     2101 cagacgtggt tcttttactt tagtatttca ggttattcaa ccaatagaaa acagagtagc
     2161 agcagcagtt aaagatcaat tctgcaaaga cctgacgtca gccgatgagt acgaaaaaat
     2221 taaaatgcat agtcttgccg tgcgtaccta caggcaacac aatgaaggaa gacctactaa
     2281 aaaagaaaga cgtgcattag atgattttct cgactggtaa atacctttaa aaaggatatc
     2341 caattccaaa attataaatc agactacgtg gtttcacttc atctgcaacc attttatcat
     2401 cacctttgta ttgagggtca acaataggaa caccaatatc caaacgaaaa ataaagaaag
     2461 taaaatcaaa acggaaaccc aagccactac ctatagctaa ttgtttgtaa aacttatcaa
     2521 tttcaaactc agcattggga cgtgatgatt cttttttacg aacccaaata tttccggcat
     2581 caaggaagaa tgcacctttc agaattttca aaatatcgaa acggtattca atattaccat
     2641 tgatttttat atcacctgta cgttcaaaat tgttcacact ggtaaaattt cccggaccaa
     2701 cattacgagc acgaaatgca cgtaaatcat ttgatccacc ggcataaaaa cttttttcga
     2761 aaggaattat tttcgagttg ccataggcaa taccaatacc agcagcaatt ctgtaaacaa
     2821 tagagttgtg tgcatttata ttctgataat atctgaaatc ggcatcaggc ctgaagtatt
     2881 gagcaaacct tttgttaaga atagtataac tgttgttcgc atccttatct gtaccttcta
     2941 tttgtctgaa ccagtaaagt gtatttccgg caaattcaat atttgttctg aaaaatttaa
     3001 aattgccagg ctcattcaat tgctgattgt tatagatgag ggtataacgt ccatttgaaa
     3061 taaggtggtt gtcataactg ctgaacagca cagggtcatt caggctttcc aatcgtgctt
     3121 tgaattcagg ctcgagcttt acattaacaa aatttacctg aaatggtgtt actatttgtt
     3181 tgataaacct acccttccgc cattctaatg atgcagaaaa atcgaggata gaacgattaa
     3241 actccggacg attctggata ttataattgg tagaaaaaac agtgacagga ttggcagcta
     3301 tatttttaaa taaacggttg aatggtttta tggctttagg caaataaatg gaattctcaa
     3361 cacctaactc ataagtattg aataacaaca agactttgtt ctctttatca tcagtaaact
     3421 tcttttgcga ttctaatgca cctctgatct taaactcaaa catctctgca cctctaaaaa
     3481 catttttgtt tctataagta aaatcacctg caacacctag gttgccaccg ttattggtac
     3541 cttcaagctc tgttttatac tcttgttttg gcgttgtacc aagattaata aaacaatcaa
     3601 gtctattgct gtcaacaacg ttatagttga tgtttacaaa acgatacata cctaacaggt
     3661 taagtccttt ataggtatag tctgcattac tctgcctgaa aacagcttcg ggtttaagaa
     3721 atgtatgatt aacgattacc tccggtttta cgggcattct ctttcctgct cgtttcacaa
     3781 acaaataacc tttataggct actgtatcat attgaattaa tgatgtatta agacttaatg
     3841 gatcatagtc tgggctgaaa attatccgat tcaatttaaa ctttgtatgt ggtaatgcag
     3901 cggaacttat tgttgaatcg gggtttgaaa atgctgctac atcctgaaat acggctatat
     3961 tttttgtagt atctgatgta taaacacgat aagaaacaaa ctgcggtgaa aactgaaaat
     4021 aaccttcgtt gcgaagtttt tttgtcagtc gttcgcggtc gccttgcaat atgccttcat
     4081 tataaatgat gcctgcttgc agctttgagg ttgaagtatc ttttattaca agagaatcga
     4141 ggttatcgtc tgcacactct cttgccacat attttataaa agaaggttta tgtgcaacaa
     4201 tggtgtaacg aacaatagct tttttctttc tgtaaatggt actgtcttta acttctgcat
     4261 caaaatagcc attcttagac ataaactgtt tgatctgttg tgttgacttt ttggtaaaca
     4321 gcgtatccaa aacttcaggc tcctctccta ccgtattctt taaccaattt ttaaattttg
     4381 ttggcttacc ccgattaccc atactatata cagcaagatg aaaacgaaac aaccctaaaa
     4441 ttctcctatt ggctttttgt ttgataattg ctttaacacc ttcgcgcagt tcgggcttgt
     4501 ctattattac ctgattgcga ttcagcaaat actcgccttt gtccaattgc ttaaaggggc
     4561 tgcaagatgc taccagtaaa atcagagtaa ttaaaatgcc gtactgcctt ttgcgcatat
     4621 tttttcagat gcggtaaaaa taaaacttat tgatcatcta tctttatacc ctttaacagt
     4681 attcttctaa tgctgacaag ttcagaaata aaacagataa agtcattaaa ggacaaggca
     4741 agccgtcatg agcaacaaaa atttgttgtt gagggcgaaa aaatggttac agaactggct
     4801 cattcagctt ttaatattat agctgtttat gctactgatg agtggattgg acaagcaaat
     4861 aatgaaatat taagtctatt aaatgaaaaa ctcatacagg ttaaaacaaa agaattagag
     4921 cggatatcat cattaaaaca acctaatgga gttttggctt tagtagccat gcggcaacaa
     4981 caggaaaccg aaataaaatt taatgatatc attctggcat ttgatgacat cagcgacccc
     5041 ggtaatttgg gtagtattat cagaattgca gattggtttg gtattaagca aatagtatgt
     5101 tcatcaactt ctgttgatct ttataattcc aaaacaattc aggctacaat gggctctatt
     5161 ttcagagtga atgtttttta tacagatgtt gaaaaatgga tttctgccaa taaacaaaat
     5221 gccacttttt atgctaccat gcttaatggc aagtctgttt attctgtcgc taagaaaaaa
     5281 cctgctgttt tggttttcgg aaatgaatca agaggtttat cgcattcaat tcagcaattg
     5341 attgatctgg atatttccat tccaaagttt cctgatgaaa aaagcagctc tgtagagtct
     5401 ttaaatattg ccatgtctgc tgcaattttt tgcagtgaat ggcgcaggga ttaaaagtta
     5461 ggtttcagta aatatttact gtagaatgca tcaatatgcg actttgcttc atgcgcttca
     5521 tcaactaatt taaagagtga aaggtcttca ggtaaaatat tgccttcatc aaccataact
     5581 gtttgcagcc attcaaacaa tttaccccaa tactctttgc caacaagtac aatcggaaat
     5641 ctgccaatct tttttgtctg aataagtgtc aacgcttcaa aaaattcgtc taacgtacca
     5701 aacccaccgg gaagtacaac aaaaccctgg gcatatttgg taaacattac cttgcgtaca
     5761 aagaagtaat caaacatcag attcttatca tgatctacaa agggatttga tgtttgttca
     5821 aacggaagtt taatatttaa accaacagat tttccaccgg cacgttgtgc acctttgtta
     5881 cctgcttcca taatacccgg gcctcctccc gttatgacac cataaccgga ttgtgtgagt
     5941 tgataagcta tttcttctgc cagtaaataa tatcttgtat ctggttttgt tcttgccgaa
     6001 ccaaatattg aaacacaagg gcctatgcgg ctcatttttt caaacccttc aacaaactca
     6061 ctcattattt taaaaatctg ccaggtatcc tgagctttaa tttcctgcca gtctttatct
     6121 gaaaatgctt ttctgattct ttcttcttct gatgctgtca tgcgatttta tttttaaaaa
     6181 atgattctta gaaaaagaac catccggtaa taaattaccc gttcgtttgt ttttacgaat
     6241 aatgctaaaa aaagttcagt agatgctgtt taagaaattt ttgctgaaag atattccctg
     6301 ttcatacggg caatattttc aatagaaatt tcttttggac actctgcttc gcaggcgcct
     6361 gtgttggtac aattgccaaa accttcatcg tccatttgtg caaccatttt cattacacgc
     6421 tcatgccttt cggcctttcc ctgaggcaac aatgaaagct gcgatacttt tgctgaaacg
     6481 aatagcatag cagaagaatt tttacatgct gcaacacaag ctccacagcc tatacatgct
     6541 gcagcttcaa aagctttgtc agcatcatct tttggtatta aaatgctatt tgcatcctgt
     6601 gcatttccgg tattcaccga cacaaaacca ccggcagcca ttattctgtc gaatgatgaa
     6661 cgatcaactg ccaagtcctt aattacagga aatgctgcag agcgccatgg ctcaacaaca
     6721 atagtgtcac catctttaaa agacctcatg tgcaactgac aagtagtgac cccttgcaat
     6781 ggcccatgtg gacgaccgtt aatatacatg ctgcaagcac cacaaattcc ttcacggcag
     6841 tcatggtcaa atgcaacagg atcttttcct tcgttaatta gtcgttcatt caacacatca
     6901 aacatttcaa gaaaagacat gtcttccgac acatcctgta cctgatatgt ttcaaaacga
     6961 cctctctcat ctttatttct ttgcttccaa actttcaggg tgatattcat acctgtatta
     7021 ttttaatttt ttctgataaa atattattta taactgcgtt gtgcaatttt aatgttttca
     7081 aatttcaact cttctttatt cattttccat tgcgaatcgc ctgtcaactc ccatgcagct
     7141 gcaaatgcaa agttatcatc gtcacgcata gcctctcctt cttctgtctg atgttcttct
     7201 ctgaagtgac ctccacatga ctcattacgt tgcaatgcat ccatacacat caactcaccc
     7261 aactcaagaa aatctgctac tctgccggct ttctccaatt cagggttaaa ctcattcatc
     7321 tcacccggaa tacgcacatc tgcccaaaac tgttttctga gctcttgtat ttctgcaata
     7381 gcttctttta gtgcaactgc attacgagcc attccacatt tttcccacat tatttttcca
     7441 agctgacgat gaaaatgatc aacagatttt gtccctttaa tctgcatgag cttttgcaat
     7501 gtttctctca cacggttttc tgcagcaaca aactcttcat ggtcggtgct gatggctttg
     7561 gttctgattt cgctgctgag atatgctcca atggtataag gaatcacaaa atatccatca
     7621 gcaagacctt gcattagtgc agaagcaccc aaacggttgg cgccatgatc gctgaagttg
     7681 gcttcaccaa gtgcgtaaag gcccggtacg gtggtcatca gattatagtc aacccacaat
     7741 ccacccattg tatagtgtac agcaggataa atcatcatcg ggctgtggta aggatctaca
     7801 ccagtaatct gtttatacat gtcaaacagg ttaccgtatt tttcttttac tactgcttta
     7861 cctaattcaa atagttgttc ttctgtaggg ttctctatac tattcatagt agcctccatt
     7921 ttaccatact tatacttcat gttataagca aagtcaaggt aaacagcttc gcctgtagca
     7981 ttaacaccaa agcctgcatc gcaacgttct tttgccgcac gtgaggcaac gtctcttggc
     8041 actaaattac cataagcagg atatcttctt tccaaataat aatctctgtc ttcttctttt
     8101 aaatcattag ggtttagctt gccggcacga atagcttctg catcttcctt tttctttgga
     8161 acccaaatac gtccgtcatt tctcagcgac tcgctcatca gtgtaagttt cgactgatgt
     8221 tcgcctgaga caggaataca ggtcgggtgt atttgcgtga aacaagggtt ggcaaaataa
     8281 gctccatttt tatgagcttt ccatgctgca gttacgttgc tgcccattgc attagtcgag
     8341 agataaaaca catttccata accaccgctg cacatcagta ctgcatgtcc gaaatgtctt
     8401 tccaactctc cggtaattaa attacgtgca atgatgccgc gacattttcc gtctatcttc
     8461 acaatatcaa gcatttcatg acgagaatac attgtaatag cacccatacc aacctgacgc
     8521 attaatgcac tatatgcacc caacaataac tgctgaccgg tttggccggc agcataaaat
     8581 gtgcgctgta cctgtacacc gccaaaagat ctgttactta acaatcctcc atactcacgg
     8641 gcaaatggaa cgccttgtgc cacacattgg tcaataatat ttccacttac ttcggcaagg
     8701 cgatatacgt tggcttcgcg cgaacggtaa tcaccgccct taacagtgtc ataaaataag
     8761 cgataaattg agtcgccatc attctgatag tttttagctg cattgatacc accctgcgcg
     8821 gctatactgt gtgctctgcg tgccgaatcc tgaaagcaaa aaacttttac tttatatcct
     8881 aattcagcaa gcgaggctgc tgcagaagct cctgcaagac cagagcctat aacaatgatt
     8941 tcgaggtttc ttttattggc aggattaatc aatggcaccg ttgaacggta tttactccat
     9001 tttttttcta tctcaccggc aggaatatgt gcgtcaagta tgttgtttgt cataattttc
     9061 agtaagtatt atcggttaat aaaaaaatat agaggcatag ctgcaaaact tgcaggaata
     9121 ataatggcaa aaacccatgt tcctattgac tgtattagtc cgttgtattt tctgtgattg
     9181 agtcctaaag attgaaatga acttttaaag ccatgataaa gatggaatga aattgctgcc
     9241 atactcacaa catagataag caccatccac cattccttaa actcgtcttt aacctcttta
     9301 tataaatctt ttacaattac atacttatga tcaccaacaa tcatctcttc catttttgaa
     9361 cccattttaa aaccggcttc catttctttt acttcaacta atttgccggt agtaagttct
     9421 gttttataca tggtaaatgg aatatgtccg aatttatact gataccaaaa atcattcaaa
     9481 tggataacga taaaaaataa aatcacggta cccagtaaac ccatatttcg tgatgcccat
     9541 ttgctattag cctttccatc atacttggca taagatacag ggcgggcttt tttgttttca
     9601 agcaccagca taattcctct tatggcatgt aaaactattg tgatgtacaa cagatatgaa
     9661 atggttttaa tgaatgggtt ggtagtcatt aatacagagt attggttaaa tgacaaaccg
     9721 gaatctgttt taaacaactg gaaatttcca ctaacgtgaa ccaccaggaa tatacttaaa
     9781 aaaagccctg tcagtgccat gataactttt ttacctatgg atgatttaaa caggctaagt
     9841 acactatttg acgacatagc tattaattta aattagtttt taattgatgc gcaaatgtag
     9901 agattacgaa aaccaattgc aattcatgac tcagtatatt tcaacaattg aactgtatga
     9961 tttttatcaa cactcatcat aaataatagg ataaacaatt tgctgcatca aaaaattgcg
    10021 aattaagttt cggagaatta tctttgcaaa aattatttga aacaatggga catcaccacg
    10081 tagaagaaaa aggacagact tttttatatg aaggcggcac tggcgcattt actgcagtat
    10141 ggatgatttt ggtttttgcc tttatattct ttctgattta cttaggataa ttttaaattt
    10201 atcagccaaa acaggctgac tttttttaac cattccacaa aattgtggct gtgtggacat
    10261 attctttcat tagtttttat tactccacta tattcacaga ttccaggata tatttctctg
    10321 cgtccattcc ataataacaa tgcttttttg ttaaaaactt cctttttgtt caacaagcat
    10381 tttaccgagt tttggagcat aatttttagc tcatgcccac aacagaacag cttattaaaa
    10441 tcagcacaca agtcagacgc gatattgttc gcatggtaca tgcccaccaa agcggacacc
    10501 ccggtgggtc tttaggttgt gccgattttc taactgcact ttatttcgaa atcatgaaac
    10561 acaatcctgc aggttttagc atggatggta aaaatgagga catctttttc ctttcaaacg
    10621 gtcacatttc tcctgtgtgg tacagtgttt tagccagaag tgggtacttc gacataaaag
    10681 aattatcaac attcagaagc atcaacagtc gtttacaagg gcacccaaca acacatgaag
    10741 gcctgcctgg agttcgtatg tcgtcaggtt ctcttggaca gggacttagc gttgccattg
    10801 gcgcagctca gtcaaaaaaa ttaaacaacg acagttctgt tgtttattca ctgcatggtg
    10861 atggtgaatt gcaggagggg cagatttggg aagcaatgat gtatgctgct gctaaaaaaa
    10921 ttgataacct tattgcaaca gttgattata acggtcagca gattgatggt gctgtgaatg
    10981 atgtgctttc attaggcaac ctgaaagcaa aatgggaagc atttggatgg gatgtgctgc
    11041 aaatgaatgg aaatgacatg aagcaagtag ttgaaacgct tcaaaatgca cgtcagcata
    11101 caggaaaagg gaaacctgtc gttatattga tgacaactgt tatgggttat ggtgttgact
    11161 ttatgatggg atcacataaa tggcatggca tagcaccaaa tgatgaacag ttgaaacaag
    11221 cacttgcaca actgccggaa accataggcg attattaatt gtatgtcggt aataaaaagt
    11281 tttatgcagc atttcaggaa attatttttc ctcgcagcat ttttcatagc ctttacatca
    11341 actgtcaggg cacaaaaaaa taattctttg ccacttacac gcattgagtt tttatttgat
    11401 gcatcgcaga gtatgtatgc acaatggcaa acaaataccc gttttgaaat tgccaaaaag
    11461 ctattgtctg aaatggttga cagcttagat aaaatcacca atgttgaaat ggctttgcgt
    11521 gtttatggtc acactaaaaa atttcctcct caggattgtg acgatacaag acttgaagtt
    11581 ccttttggta aaaacacagc ctatctgatt aaaaaaagat tgagtgaaat aagtccgagt
    11641 ggcaccactc ctattgcaca atcgctcgat caatgtggca atgacttccc aacagatcct
    11701 gcacgaaaca ttattattct cattacagat ggaatagaag aatgtaatgg agacccatgc
    11761 gcagtttcac tcatgttgca acgaaaagga attattctga aaccatttgt tatcggcctt
    11821 gggctttcca aagaatataa agacgttttt aattgtgtag gtaactatta tgatgcagtt
    11881 gatgaaacag gtttccgaac tgtattcaat gttgtgattt ctcaagcgtt aaacaacact
    11941 accatacagg ttaacctgct ggatgaaaaa cacagaccta cagaaaccaa tgtaaacatg
    12001 accttttatg atacctattc gggtgcctta cgctataatt ttgtccacac attgaatgca
    12061 aaaggactac cggacacctt attaattgat cctttaggca cctacgacat tgttgtaaac
    12121 accataccac cggtaagaaa agacagtaat aaaattgttg caggcaaaca caatacaatt
    12181 ggtattgatt gtccgcaggg tgatttattt ttaaaatttg acggtacaag cgactataaa
    12241 aatcttcaat gcattgtcag aaaaaaaggt gacagtaaaa cactgcatgt tcaagcattt
    12301 aacactacca cgcgctacct caccggagat tatgaccttg aagtattatg tctgccaagg
    12361 atgattatta atgatgtaaa tattgcacag agtaaaacca caaccgttca gattccaaat
    12421 ccgggcatgg caacttttca aactgtttct gccggttatg gaagtgtata tcaggaaaaa
    12481 gacaataaac tgaattgggt ttgtaatctc agcgaaaaca acatgcatga aagtatatta
    12541 ttacaacccg gaaattacag agtagtattc agaccaaaaa tatctaaaga agcaatttat
    12601 actatagaaa aaacattccg tataacttcc ggatcaggaa ttacagtaaa catcaacaaa
    12661 taattgagta atgaaaaaat ttacctacac cgaaaaaaaa gatacccgtt ccggctttgg
    12721 cgcaggattg ttagaagcag gcaaggccaa ccctaatgta gtagcattat gtgctgacct
    12781 tacaggttca ttaaaaatgg atgcatttga gaaagcattt cccgagcgtt tttttcaatg
    12841 tggcattgca gaagctaaca tgatgggtat tgcagcaggt atgactattg gtggaaaaat
    12901 tccttttaca ggaacgtttg ctaatttttc tactggaagg gtttatgatc agataaggca
    12961 atcaattgcc tattctggca agaatgtaaa gatatgtgca tctcatgcag gtttaacatt
    13021 aggcgaagat ggtgcaacac atcagattct tgaagacatt ggcctgatga aaatgttgcc
    13081 gggcatgact gtaattaacc catgcgacta taatcaaaca aaagcagcca ccatggctat
    13141 tgcaaaacat ttcggacctg tttatttacg tttcggacga ccatcctggc cgatatttat
    13201 tcctgagaat acaccttttg aaataggaaa agcattgctc ctaagcgaag gttcagacgt
    13261 cagtattttt gcaacagggc atttggtctg gaaggctatt gaagcaggaa tggcacttgc
    13321 cgacatgggc attagtgcag aaataataaa tattcacacc attaaacctc ttgatgaaaa
    13381 agcagtcata caatcagttt caaaaaccgg atgtgcaata actgcagaag aacatcagat
    13441 taacggaggg cttggagata gtatttgtca gttattgtcg cgtcatttgc cctcacctgt
    13501 tgaaatggtt ggagtaaaag acaccttcgg tgaaagtgga acacctgatg cgctgatgac
    13561 taaatacgga ctcgacagta agaatattgt ctttgcagtt gaacaggttt tgcagcgcaa
    13621 aaaggaattg gcaggctaat tttttccctg tgaaacaccg ggaatctttt taaattgaaa
    13681 taataggata aatcggtttt tcattgaatc actactcgga taaagaacta cttgagcaat
    13741 ttagtaacgg caatgccgat tacgcattta atctcattgt cagaaaatat cagcagaaga
    13801 tttacactca cgtaaggcgt ttggttgctg accatgatga tgccgatgat ttaacgcaaa
    13861 caacttttat taaagcatgg aaagccttag gaaatttcag agccgatgca cagctttata
    13921 catggctgta tagaattgct accaatgaat gttacacttt tctgaaaaac aaaaggaaac
    13981 ggttttttat tcccatccat gatgttgaag ctgaactggc agataaatta gaacatgaac
    14041 cgtctttaaa agccaatgag ttggagctta aacttcaaaa agcaatatta tctttaccgg
    14101 aaaaacaaag ggcagtattt aacctcaggt attatgatga aatgaaatac gaagacatga
    14161 gcgaggtttt aggtacatct gttggcgctc ttaaagcatc ttaccatcat gcagtgcaaa
    14221 aaattgaaaa attcttacaa tcattttaaa cctttttaaa tcaaaaacat caaagtagta
    14281 tgaaagatga aatttacaat gaattgaagg ataaagcacc gttgctgtcg tcaatagaaa
    14341 agaagcatct ttttgatact cctgaaaatt attttgatca actgccctca gccattcaac
    14401 aaaaaattaa acagccgcaa accagcacaa ttaaaacttt attctcaagg ttgctgaaac
    14461 cgcaatttgc aattggattg tctgttatac ttttggttgg aggtttttat ttattcaatc
    14521 aacacaaagc agataatcaa tatgcatttg ttgcagtaaa tgttgattct ttaaatgtca
    14581 atgaactgag tgacttgtta gataccgatc agttagaatc tattgcaccg gcagataaca
    14641 atgctgatgc tgaaactata aattatctta ttgaaaacta taatgactta agttatgata
    14701 tcaacgacat ttaaaatgat gaaatcagca ggaattgctg tgtttatttt gctttttgca
    14761 ggtatggcaa tggcacaacc cggtcaaaag aagatggaca gaagggataa ggctgaagct
    14821 atgaaaattg gattcatcac caacagactg agcctaacac aagatgaagc aaagaaattc
    14881 tggcctgttt ataatcaatt tcaggatgag ttaagttcac tacgacaaaa acgcagaggt
    14941 gacaggcgtg aagccaaaca ggattttgat caattgtctg acaaggaagt tgaaaaagta
    15001 gttgatgatg agattgtttt tcgtcagagc gaattggata tcattaaaaa atatcatgcc
    15061 caatttaaac aggttcttcc tattaaaaaa gttgccagat tgtatcaagc tgaagaagat
    15121 tttaagaaag aattacttcg tcaacttaaa gacagaagag atgcacgagg taattaatca
    15181 tttataatgt ttagtaatag attaagcggg caatgctcgc ttttttattt gtataaagaa
    15241 tcattttacc caaaaaacta actatgatta attattatcc ttcattttta actcaaattc
    15301 tctgcttttc accaaaaaca aatctacatt tttaatgcct atacagataa aatcaacctt
    15361 cgccctgtaa aaaagcacat ttaccaaaac aacctaacat tgcatacatg catgtttaat
    15421 tttacatccg ttatacacaa caacacatca tgtatatgtc aacagaggta acagcgggaa
    15481 taaaggtaac ggtagaaaca ttctttcaac ctacccattc caaccctgtt cagcatcatt
    15541 atgtattcgc atatcggatt accatttcta acgaaagcga agaaagcatt aaactaaaac
    15601 gcagacattg gtatatcatt gacagcgaca atgccagacg cgaagttgaa ggcgaaggcg
    15661 ttgttggaga acaacctgtt attgaaccgg gtcagcttta ccgatatgtt tcgggatgca
    15721 acctgaatac tgaaatagga aaaatgtatg gcacctatct aatggaacgc cttagcgata
    15781 ataaaatgtt ttatgttaag atacctgagt ttagaatggt tgtgccttca aaaatgaatt
    15841 agtcccaata gttatttgat ttgaattttt aaaaagagag ccgccaggct ctttttttat
    15901 ggataaaagt tagttttgca taaagtttca ccatgcacct tatagcacct tccattcttt
    15961 ctgccgattt ttcacaactg agtaaagatg tggaaatgat caacaacagt gttgcagatt
    16021 ggtttcatgt tgatgttatg gatggtgttt tcgtgccaaa catttctttt ggatttccgg
    16081 taataaaagc cataaagaaa catgcaaaaa agcctttgga tgttcatctt atgattataa
    16141 aacccgagag atatgttgaa cagtttcgtg aagcaggtgc tgatgttctt accgttcata
    16201 tagaggcatc tgttcatctg caccgaacaa tacagcagat aaaaaaagaa ggtatga
//
LOCUS       DAOOOL010000051        15996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_306_length_15996_cov_95.022646, whole genome shotgun sequence.
ACCESSION   DAOOOL010000051 DAOOOL010000000
VERSION     DAOOOL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15996
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_306_length_15996_cov_95.022646"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3455
                     /locus_tag="PKU82_05835"
     CDS             <1..3455
                     /locus_tag="PKU82_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ82430.1"
                     /translation="VYLNDSLIYWDNNAVSFSYNQVKNGINQQLIQLKNGWYELFRYQ
                     RRNITIIGLLPIRAGYDFQNKYLRNEFNPVLGIPEHALLLPSPATGSYPVISSDGNYL
                     FSIKYNPIMSDPGRLWYIAALYLAGFFLLLISFYLYARQWLLKNPLVSFVIISLLVFI
                     KWLMLTFRFPEFLYGMPLFNPKFYATSYFLNSLGDFLLSASVFCCVILFVYNYLHLRR
                     RKIKVIRQSPTLLSIVVIGNLLFTFLFSVFINYLISGLILNSKISFNVNNVFELTFFS
                     LIGFLIIGILLFSFYVSCEGTVKFAEYSGFSLPYNFLLFLITQGLFLVVLILFRNTDV
                     FVNYGVATFFLTNSLILFISYIRFTSRQNFSFVRYMFVIAGFSVYAAYTISSFNMVRE
                     KNNMRLLVSKVENREDLIAEYLFQEIDQKLKTDPVITSNIIQGRSPLLEEKIKKRITL
                     NAFNQAYWTRYDIQIKAYDSAGVSLFANSDTLRNVTFYDSIINTQTRPTYSHSFNYLS
                     SNSGKIGYIGKITYPKNASGELAATIIIQLDSKFNQEEGGFPDLLLSEKMPASRDLSK
                     YAYAKYENGKLIFQSGKFNYFHSAATYDRMFGLVKSDQFVSLGGFIHLFYNQDKNNLI
                     VISYKTPQAIEQVTLFSYVFIFFSATFILIYMIWLFLANNFRLHLDFRKRIQLSVVGL
                     VVIAILLIGGGSIFYISRGYSEEQKVRIKEKLSTLMLAVESEFNDKNLTEANLTEETT
                     LNFSRISNTLSSDFNLYDKNGRLIYSTQPKIFDKEIISRLMNRSAFDQLKNYQTNGFI
                     HDEKIGLLEYTSAYETIVNKDNKVAAYINLPYFARQNELKKEISGFLVTLINIYVLLF
                     GIAIVITFIISNRLVKPLNLIQQKLARVKLDSSNELLEWEGGDEIGSLVNEYNKMVNK
                     LAKSADALAQSERETAWREMAKQVAHEIKNPLTPMKLSMQHLARAWKEKNSNFENIFE
                     KVSRTMIEQIDSLSRIATEFSNFAKMPKGNFEKIDLVKSIQSNIDLYSEVRNVDFVFN
                     KSNIDDEAVVNGDWEQTLRAFGNLIKNAVQAIPENQQGLITIQLTKADKQFYISITDN
                     GKGITEEAKQKIFTPNFTTKSGGTGLGLAMVKSIVENMQGKISFESEYGKGTTFKIEL
                     FEAD"
     gene            complement(3458..4681)
                     /locus_tag="PKU82_05840"
     CDS             complement(3458..4681)
                     /locus_tag="PKU82_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ82431.1"
                     /translation="MRRIIFCFLILFSVQGYAQQVLTNYTIANSPLPENSVRCIEVDH
                     IGRKWIGTDYGLAVFDDVNWTVYLPFASGLPANSVRAIAFDSLNNAWIGTLGGGVAKF
                     DGTNWTVYDDTNSPITENSIRALAFDSAGALWIGNQDGLVKFDGTNWIVYSAASSIIQ
                     NTIASIHTSKNRKVYAGSVNGGLFILHEDTIKSNLNISNGSGIPDNTILGFDEDSVGN
                     IWMATPANGIVVYRNIGGWFWYYMGSSFIQSNSLSDIQISNDHQSVWVATADSGIIRK
                     TGTVYTCFNTVNSSMPDNNIQCLTIDSLNNIWIGTATQGVVKFQDFTGIRNVSSVNDF
                     DSYPNPVTDKINFKTENIPFTIEVMDAKGAVVYSEIINSAMGNIDVSHLKNGIYSFKA
                     VSKNNVAVIKRFCKQ"
     gene            complement(4737..9161)
                     /locus_tag="PKU82_05845"
     CDS             complement(4737..9161)
                     /locus_tag="PKU82_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HOZ82432.1"
                     /translation="MKRIYLLLVVLLFASQAIYAQCTLTNASTCSCLGGGTNCDLLPD
                     ITASRKPLTILGSNGVIEYSQSGNGVNDGRLRITVSTPNIGHGPMETRSSNIFVCGTD
                     TFVGTAPSICPDNITYPKIIINQRIYHKNGNSMSYYDRPAGTMTYHPSHGHQHVDNWG
                     VYTLRTPTADPNPVNWPIVGNGAKLAFCLLDIGACASSNGSGLCIADNGDTLDTPAEF
                     YGNYGLGGGSYGCSATLQGISAGYFDTYNQSLDGMWINIPPGTCNGTYYIVCQQDPDN
                     YFLEENDNNNVMAVPFTLTKQGGVAPTITANGPTTFCAGGSVTLTCSGASDYLWSNGA
                     TTQSITVSQGGTYTCTVNNTSTCATASTPVTVTVNSFPVTASTSSANVCAGDPVQLNG
                     SATSSGTVTVVQTFSNTTAYPIPDNNSTGVQSPITVSGINPTALSSGVVVSVNANITH
                     TYDGDIELRLVAPSGQYAVLSNRRGGSGDNYTNSTFAMNGPMAITSGNPPYNGTFIPE
                     GNFNSLTGNVNGVWKLHVIDRASVDVGTIQNWTLKINSQVPTSISYNWTSNPSGFNST
                     NQNPIANPTQTTTYTVSAVESTTGCAGSGSVTVNVANPNVSVSGSNTICEGTSTTLAA
                     NGATTYAWSPATGLSATTGSSVTASPTQTTTYTVIGMMNGCSDTTQFTVTVNPAPGTI
                     SATASSSPACIGDLVLLEAFNADVTSWSWTGPNNYSSTQSSNTILNIQPNQAGLYTVT
                     GSNACGSSSAQVNIVVNPTPYSQVTLTYPNGHCAGSYANACASDTSNGAYAPYTYLWD
                     NGQTTSCINQQILANPLPLQGPSVTITNSLGCTSTNIAVWNYPFGPSADSVQNPQTIC
                     QGGSYSINGNNYSTAGTYYDNFINVSGCDSIVITQLTVTPSLSINVSGNNTICEGQST
                     TLTATGASTYSWSPATGLNTSTGATVDATPAATTIYVVTGTSNGCSNTATFTVNVNPN
                     PVIQTSGNVDVCSGQFVTLSASGASNFTWSPSTALNTTTGASVVANPISTITYTVVGT
                     DNGCSASSSITVNIIAQPVVNVSASTTSICNGQSATLTASGADTYTWSPSTGLNTTSG
                     STVIATPSTTMTYTVTGFVAGGCFSTNTITINANTGTAPTIPGLIAGNKKPCPNVNEN
                     YSVSPVANAVSYTWTVPAGVTIISGQGTNNIHLNFASNFTSGNISVVANGACGVSASR
                     SSSLSLNIPATPSSITGPVTGLCDAAGTFTSATVSGATSYTWSVPAGVNIASGQGTKT
                     ASIYVGSSFTSGNVCVTADNTCMSSKPRCVAIKKTPSVPSTLTGPTTVCAGQQNVSYS
                     TNTVFGATNYQWIVPSGSVITSGQGTPNITVDFGTKTGSVGVNAYNACGQAGRRAVLV
                     SFNCRLGEMSSSTSVIEISPNPTSTGMVNLTVNSEVNGIALIKMTDILGKSVLIKNIA
                     NVIGTNNHKLDLSKFNKGIYLLTVDTGSKKQTLKVVIQ"
     gene            complement(9295..9465)
                     /locus_tag="PKU82_05850"
     CDS             complement(9295..9465)
                     /locus_tag="PKU82_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82433.1"
                     /translation="MQHLFRCYKSTGRKKIYLFGILRKIYTGFNGPATGAYHEKTKDD
                     FIRIITTLLSEK"
     gene            complement(9496..9885)
                     /locus_tag="PKU82_05855"
     CDS             complement(9496..9885)
                     /locus_tag="PKU82_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82434.1"
                     /translation="MDTAINNYSADYASNKFYENKYRQHYKLLEAKFPVNILTLDFDS
                     SVLKAAEIDSMLDVWFSPKYPFLDEPHNCEDNDICVEIRYSQNLKKFLYRGDYDPENY
                     DKVAFKTLTVSFFLKKNKQYYNEQKQH"
     gene            complement(9967..10344)
                     /locus_tag="PKU82_05860"
     CDS             complement(9967..10344)
                     /locus_tag="PKU82_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82435.1"
                     /translation="MTTTNHTMRKLYLVISALIVFFSSCNSNSNQAISESYIPIFADT
                     TGWFFSKDNLVINLNIKYINDAYKFEKQLGSKISYLAQGVNFKTNFNVPTTYYDSIMS
                     PKEIPAFVIFHRPEKYEFPLNVI"
     gene            complement(10341..10922)
                     /locus_tag="PKU82_05865"
     CDS             complement(10341..10922)
                     /locus_tag="PKU82_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82436.1"
                     /translation="MFNNLFLFVSILFLANCRSDSKPQNISGILFKDSLNTLSIYIPK
                     RFSIVDSIYEKEILGDKFEFFSATFNGDSSKIDEFIKYCNSLIVGVEFNSPVKSLAEI
                     DSLDEHAPTFQVRIKYTLRTKLKSDKKNHFLIKQKFFKSSGEFNIQVVNAYCLHKSDL
                     FFLQSIFYYSNKAKTEKNYKEIIGVLESLEFIN"
     gene            complement(10912..12345)
                     /locus_tag="PKU82_05870"
     CDS             complement(10912..12345)
                     /locus_tag="PKU82_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ82437.1"
                     /translation="MIEISQIKYLCFEGGGGKGLAYLGVIQALEEIINKKMGPPEGGT
                     PYNVTSENKTPIIRISQPIKQRNILGVSGSSAGAITAFMVAMGMSHYEILQETNKVVS
                     LSLGNFTIAQKDVSVTQFESFFDSPDLYNYRTNNKKSPLREYNNLSMPIKIIGNVLSE
                     FLNVFKWTFKPFVSENEIFVRKILLTEGTPARALNLKKYFLGIIYNRGLLSGVASKKY
                     FSDLIENYLFPKLSTLKMKKLKSRGEDITFQDFFYMTGVDLIVTGTNISLGKPKYFSA
                     AFTPDFSVVDAISLSMSIPFMFRPYLIESEVNLDSNLPADYNKDYLGLYVDGGMLNNY
                     PLHAFDYRPQKNINYQGNFDNYPLASPNISSSATEFCDCILGIRLQNLVKEYEVSDDF
                     KEGNSVIIKDFINQLYNTILYPSEEGQIRSVLENNLSVSIDVTDLELLDFSNPLINRQ
                     YRNLNSLAEKQEKVIENACKKIYDNVQ"
     gene            complement(12357..12593)
                     /locus_tag="PKU82_05875"
     CDS             complement(12357..12593)
                     /locus_tag="PKU82_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82438.1"
                     /translation="MYYLSSGGEIWNGEDVPSPTDPLYISIIDEMKEFDGQFVGGNQE
                     GEPWIQKAPTNLVYLTKLDGSSPGLPDFSDELLP"
     gene            12841..13373
                     /locus_tag="PKU82_05880"
                     /pseudo
     CDS             12841..13373
                     /locus_tag="PKU82_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655221.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13681..14853)
                     /locus_tag="PKU82_05885"
     CDS             complement(13681..14853)
                     /locus_tag="PKU82_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOZ82439.1"
                     /translation="MSSTALHGLIIASGIWRAVLMTPGGELPFNFETREVNSRSEIFI
                     KNGNEEIEVNEISYANDSLIIQLPVYDSEFRVRVSGDSMIGFWYNNSRKNYAPTPFKA
                     FLGEKYRFFNNTKSEIKLPEKWEWKFSPGTKDSSQAIALFKQNGTDVRGTLLTESGDY
                     RFLDGCLNNDSLFLSYFDGAFAYLFKGSIKNNMMQGVFYSGHHWQQPWQAVVNESAEL
                     PDPFKLTFLKNDTASFSFSALRLDSTVFSYPNKDLKGRVVVVEMMGTWCPNCMDETAF
                     LSDYYSRNKSQDLTIIGLAFEKTSDFRKSVGNVKRVKEKYHVQYETLIAGNREGAASK
                     MSMLSKINGYPTTIFIDRKGKVRKIYTGFNGPATGAYHERTKDDFIRIITTLLSEK"
     gene            complement(14870..>15996)
                     /locus_tag="PKU82_05890"
     CDS             complement(14870..>15996)
                     /locus_tag="PKU82_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOZ82440.1"
                     /translation="EGGFEREKRSFKSSDSPRPYRKRDEGFEGDEKPRFKKRSDFGGS
                     GKFDKSKSFKRRDDNVEGEDKPRRFSKSFDSDTREKRSYDRKERSSGRFDKPYRKKND
                     NRKYSKKEKESSEQLSDGLVRLNRYIANSGICSRREADELITAGLVSVNGEIVTTMGT
                     KVSQDDVIKYNGETIRNEKPMYLLLNKPKDYITTMDDPQDRRTVMHLIAGACKERVYP
                     VGRLDRNTTGVLLFTNDGDLAKKLTHPSYEVQKIYHVELDRNLKSSDKEKIKEGVMLD
                     DGMATVDDIQYDGGFNDKKQIGVEIHSGKNRIVRRIFESMDYNVIKLDRVVFAGLTKK
                     DIARGKWRFLSEMEVNALKMATSKVKPKDKEPRKRISRSK"
BASE COUNT         5003 a         2900 c         2794 g         5299 t
ORIGIN      
        1 ttgtatatct caacgactca ttaatttatt gggataataa tgctgtaagt ttctcataca
       61 atcaagttaa aaacggaatc aaccaacaac ttattcaact aaaaaatggt tggtatgaat
      121 tgtttcgtta ccaacgaaga aatattacca tcattggttt gttgcccata cgtgcaggct
      181 atgattttca aaataagtat ttgcgcaatg agtttaatcc tgttttggga attccagaac
      241 atgcccttct gttaccctca ccggcaacag gatcttatcc ggtaataagc tctgatggaa
      301 attatttatt cagtataaaa tacaatccga taatgagcga tccgggtcgc ttgtggtaca
      361 ttgctgcatt gtatttagcc ggatttttcc ttttattaat ttcattctat ctctatgcaa
      421 gacagtggtt attaaaaaat ccattagtgt cgtttgtaat aattagtctt ttagttttca
      481 tcaaatggct catgctgaca ttccgttttc ctgaatttct ttatggcatg ccgctattta
      541 atcctaaatt ttatgcaaca tcatactttc tgaattcatt gggtgatttt ttgcttagtg
      601 catctgtttt ttgttgtgtc attctgttcg tttacaatta ccttcattta agaagaagaa
      661 aaataaaagt tatcagacaa agtccaacat tactttcaat tgttgtaatc ggaaatctgt
      721 tgtttacttt cttgttttct gtttttatca attatcttat aagcggatta attcttaact
      781 caaaaatttc tttcaacgta aacaatgttt ttgaattaac ctttttcagc ctcattggat
      841 ttttaataat aggtattctg cttttttcgt tttatgtttc ctgcgaaggg acagtaaaat
      901 ttgctgagta tagtggtttc tcattacctt ataactttct tttattttta attacacaag
      961 gactttttct ggttgtatta attctgtttc gaaacactga tgtttttgtg aattatggtg
     1021 tagccacatt ctttctgacc aattcgctca ttctcttcat cagttacatt cgttttacaa
     1081 gccggcaaaa tttttcattt gtacgctaca tgtttgtcat agcaggcttt tctgtttatg
     1141 ctgcatatac catttcgagc tttaatatgg tgcgcgaaaa aaacaacatg cgtctgttag
     1201 tttcaaaggt tgaaaacaga gaagacttga ttgcagaata tttatttcag gaaatagatc
     1261 agaaactaaa gactgatcct gtaattactt caaatattat tcagggaagg tcgcccttgt
     1321 tagaagaaaa aataaaaaag cgtatcactc taaatgcatt taatcaggca tattggacac
     1381 gctacgatat tcaaatcaaa gcttatgact ctgcaggtgt ttctctgttt gccaattccg
     1441 acacattgcg caatgtaact ttttatgata gcatcatcaa tacgcagaca cgaccaacct
     1501 atagccattc ctttaattac ctgagttcta actcaggtaa aattggatac atcggaaaaa
     1561 ttacatatcc aaaaaatgcc agcggtgaac ttgctgctac aataattatt cagcttgact
     1621 ctaaatttaa tcaggaagaa ggtgggtttc ccgatttact tctttcagaa aaaatgcctg
     1681 catcgcgcga tctttcaaaa tatgcttatg caaaatatga gaatggcaaa ctgatttttc
     1741 aaagtggcaa gttcaactat tttcattctg ctgctacata cgaccgtatg tttggtttgg
     1801 taaaatccga tcagtttgta tcattaggag ggtttattca cttattctat aatcaggata
     1861 aaaataatct gattgtaatt tcatataaaa cacctcaggc tattgaacag gtgactttat
     1921 tctcttatgt gtttattttc ttttctgcaa cattcatttt aatttacatg atttggcttt
     1981 tcttagccaa caatttcagg ttgcatcttg atttcagaaa acgaattcag ctttcagtag
     2041 ttggtttagt tgttattgct atattgttga ttggcggtgg ttctattttt tacatttcgc
     2101 gtggttattc cgaagagcaa aaagtcagga ttaaagaaaa actttcaaca ctgatgcttg
     2161 ctgttgaatc agaattcaat gataaaaact taacagaggc aaacttaaca gaagaaacta
     2221 ccttaaactt ttcaagaatt tcgaatacac tttcaagcga ttttaatctt tatgacaaaa
     2281 acggacgatt aatttattcc acacaaccaa agatatttga taaagaaatt atttcaaggt
     2341 tgatgaacag gagtgctttc gaccaactta aaaattatca gactaatggt tttattcatg
     2401 atgaaaaaat cggtttattg gaatatactt cagcctacga aactattgtg aataaagaca
     2461 ataaagtagc agcttatatt aacctcccat actttgcacg gcagaatgaa ttgaaaaaag
     2521 aaatcagtgg tttcttggtt acactaatca acatttatgt actacttttt ggtatcgcca
     2581 ttgttattac attcattatt tcaaaccgtc ttgtaaagcc attaaacctt attcagcaaa
     2641 aattagctag agtaaaatta gacagtagta atgagctgct tgaatgggaa ggtggagatg
     2701 aaattggctc tttggtaaat gaatacaata agatggtaaa caagttggct aagagtgcag
     2761 atgcattagc gcagtcggaa agagaaactg cctggcgaga aatggcaaag caggttgccc
     2821 atgaaattaa aaatccactc acaccaatga agctgagtat gcagcatctt gcacgtgcat
     2881 ggaaagagaa gaacagcaac ttcgaaaaca tttttgaaaa agtttccaga acaatgattg
     2941 agcagattga ttcactatca aggatagcta cagagttttc aaactttgca aaaatgccaa
     3001 aaggcaattt tgaaaaaata gaccttgtaa aatctattca aagtaatatt gacctttaca
     3061 gtgaagtgcg taatgtggat tttgttttta ataaaagcaa cattgacgat gaagctgttg
     3121 ttaatggtga ctgggaacaa acattaagag ctttcggaaa tcttataaaa aatgcagtac
     3181 aagctattcc tgaaaaccaa caaggattaa ttacaattca acttacaaaa gcggataagc
     3241 agttttatat tagtatcact gacaacggta aaggcattac cgaagaggca aagcaaaaaa
     3301 tatttacgcc aaactttaca actaaaagtg gaggcacagg tttaggactt gctatggtaa
     3361 aaagtattgt agaaaatatg cagggaaaaa tatcgttcga aagtgaatac ggaaaaggaa
     3421 ctacttttaa aatagagcta tttgaagcag attaaaatta ttgcttacaa aaacgtttga
     3481 taacagcaac gttatttttt gaaacggcct taaaagaata aatgccattc tttagatgtg
     3541 agacatctat atttcccatt gcagagttaa ttatttctga ataaacaacg gcaccttttg
     3601 cgtccattac ttcaatagta aatggtatat tttcagtctt gaaattaatt ttgtctgtaa
     3661 caggattggg gtatgaatca aaatcattga cggaagaaac attacgtatt cccgtaaagt
     3721 cctgaaattt aacaacacct tgtgttgctg tgcctatcca tatattgttc aaagaatcaa
     3781 tagtcaagca ttgaatattg ttatcaggca tggaagaatt aacagtgttg aagcaagtat
     3841 aaacagtacc tgtttttctg attataccac tatcggcagt agctacccaa acagactgat
     3901 gatcgttact gatttgtata tctgaaagac tatttgactg aatgaaagaa cttcccatat
     3961 aataccaaaa ccatccacca atatttctgt aaaccactat tccatttgct ggagttgcca
     4021 tccagatgtt tccaacagaa tcttcgtcaa atcccaaaat agtattatca ggaattcctg
     4081 aaccatttga aatattcaag tttgatttaa tcgtgtcttc gtgaagtata aacaatccgc
     4141 cattaacact tcctgcataa acttttctgt tttttgaagt atgaatagat gcaatagtat
     4201 tttgaataat gctgcttgct gcagagtaaa ctatccagtt ggttccgtca aactttacaa
     4261 gtccgtcctg atttccaatc cacaatgcac ctgctgaatc aaatgcaaga gctctgattg
     4321 aattttcggt aatgggacta ttggtatcat cataaactgt ccaattagtt ccatcaaact
     4381 ttgccacacc tccaccaagt gtaccaatcc aggcattgtt cagtgagtcg aatgcaatag
     4441 cacgcacact attagcaggc aatcctgatg cgaagggaag atatactgtc caatttacat
     4501 catcaaaaac agcaagtcca taatcggttc caatccattt tcttcctata tgatcaactt
     4561 caatacatct taccgaattt tcaggtaatg gtgaatttgc tattgtatag ttagtcaaca
     4621 cttgttgtgc atacccttgc acagaaaata aaatcaggaa acaaaatatt attcttctca
     4681 ttctttaaaa ataaaaaccc tcccgtagga gggcattatt ccggttttag atataattat
     4741 tgtattacaa cttttaatgt ttgtttttta ctaccggtat caacagtcaa caaataaatt
     4801 cccttgttga atttcgacaa gtctaattta tgattgtttg tacctatcac atttgcaatg
     4861 ttctttatta aaactgactt tcctaaaata tcagtcattt taattaaagc tataccatta
     4921 acttctgaat taactgtcag gttaaccatt cctgttgaag ttggatttgg cgaaatttca
     4981 ataactgaag tactactact catctcgcct aagcggcaat tgaatgaaac aagtactgct
     5041 cttcttcctg cttgtccaca tgcattgtat gcattgacac caacacttcc cgtctttgtt
     5101 ccaaaatcaa ctgtaatatt aggagttccc tgacctgatg taattactga tcctgatgga
     5161 actatccatt gatagttggt tgcaccaaat acagtatttg tactatatga cacattttgt
     5221 tgtcctgcac aaactgttgt tggtccggtt aaagttgatg gcactgatgg tgttttctta
     5281 attgctacac aacgaggctt gctactcata catgtgttgt cggctgtcac acatacattg
     5341 ccactcgtaa agctgctgcc tacataaatt gaagcagtct tagttccctg accacttgca
     5401 atatttactc cggccggaac cgaccatgta taacttgtgg ccccactaac agttgcactt
     5461 gtaaatgtgc ctgctgcatc acataatcca gtaaccggac ctgtgataga tgatggtgtg
     5521 gcaggaatat tcaatgacag cgaagatgag cgtgaagcac tcacaccgca agcaccattt
     5581 gcaacaactg atatattacc acttgtgaaa tttgatgcaa agtttaaatg aatattatta
     5641 gttccttgcc ctgaaataat agtcacccct gcaggaacag tccatgtata tgaaacggca
     5701 ttagctacag gacttacaga ataattttca ttcacattcg ggcaaggctt tttatttcct
     5761 gctattaaac caggtatagt cggtgcagtg cctgtgtttg cattaatggt aatggtattt
     5821 gtactaaagc aacctccggc aacaaaaccc gtaactgtgt aggtcattgt tgtggatggt
     5881 gttgcaatta ctgttgaacc tgatgtagta tttaagcctg ttgatgggct ccatgtgtat
     5941 gtatctgctc ctgatgcagt gagcgtagca gactgaccat tacatataga tgttgtactt
     6001 gctgaaacat ttacgacagg ctgagcaata atattcacag ttattgaaga agaagcagaa
     6061 catccattat ctgtacctac aactgtgtaa gtaatcgttg atattgggtt agcaacaacg
     6121 ctagcacctg ttgtagtatt taatgctgtt gaaggcgacc atgtaaagtt gcttgcacca
     6181 ctggctgata aagtcacaaa ctgtccactg caaacatcta catttccact tgtttgaata
     6241 acaggatttg gatttacatt tactgtaaaa gttgctgtat ttgagcagcc gtttgatgtg
     6301 cctgtaacaa catagatagt tgttgcagca ggtgtagcat ctactgttgc tcctgttgaa
     6361 gtatttaatc ctgttgcagg actccatgaa taagttgatg cacctgttgc tgttaatgtt
     6421 gttgactgtc cttcgcagat ggtattgttt ccactaacat taatactaag cgaaggtgta
     6481 actgtaagtt gtgtaattac tatactatca caaccggata cattgataaa gttatcgtag
     6541 tatgttcctg cggtagaata attatttcca ttaatagagt aagagcctcc ctgacaaatt
     6601 gtttgtggat tctgaactga atcggccgaa gggccaaaag gataattcca aactgcaata
     6661 ttcgttgatg tacatcctaa actatttgta atagtaacac tcgggccttg caagggtaat
     6721 ggatttgcaa gtatctgttg atttatacaa gaagttgttt gaccattatc ccataggtag
     6781 gtgtatggtg cataggctcc gtttgatgtg tctgaagcac aagcgttagc atagctgcct
     6841 gcacaatgtc catttggata agttaatgtt acttgtgaat agggtgtcgg atttacaaca
     6901 atgttaacct gagcagatga acttccgcaa gcattgcttc ctgttactgt atataatcct
     6961 gcctgattgg gttgaatatt aagtattgta tttgaagact gtgttgaact gtaattattc
     7021 ggccctgtcc aactccagga agtaacatca gcattaaatg cttcaagtaa aacaagatct
     7081 cctatacatg cagggcttga tgatgcagtt gcagaaatgg tgcccggtgc aggatttact
     7141 gtaacagtaa attgtgttgt atcagaacaa ccattcatca ttccaattac agtataagtt
     7201 gttgtttgag ttggcgaagc tgtaacgctt gatcctgtag ttgcacttaa acctgttgca
     7261 gggctccatg cataagttgt tgcaccgttt gcagcaagcg ttgttgatgt accttcacag
     7321 attgtattgc ttcctgaaac actaacgttt ggatttgcca cattcactgt cactgagcct
     7381 gagcctgcac atcctgtagt actctcaact gcagatactg tatatgtagt agtctgtgta
     7441 ggattagcta tcggattctg atttgttgaa ttaaatccag aaggattaga tgtccagtta
     7501 taagatattg atgtcggaac ctgtgaattg attttcaatg tccaattttg aattgttcct
     7561 acatctacag atgcacggtc aattacatgc aatttccaaa caccgttaac attacccgtt
     7621 aagctgttga agtttccttc tggaataaat gttccattat aaggtggatt tccactggta
     7681 atagccattg gtccgttcat tgcaaaagtt gaattggtat aattatcacc actaccacct
     7741 ctgcgattac tcagcactgc atattgtcct gatggagcaa ctaaacgtaa ttcgatgtca
     7801 ccatcataag tatgtgtaat atttgcattt acagacacta caacacctga acttaatgca
     7861 gttggattga taccactaac ggttattggt gattgtacgc ctgttgaatt attatcagga
     7921 atcggataag ctgtagtgtt agaaaaagtt tgtacaacag taacagttcc tgaacttgtt
     7981 gctgagccat tgagttgcac agggtcaccg gcacaaacat tagcagaact tgtagatgca
     8041 gttaccggga aactattaac cgttactgta accggagttg atgcagtagc acaggttgaa
     8101 gtattattta ctgtacaagt gtaagttcca ccttgtgaaa cagtgatgct ttgtgtagta
     8161 gcaccattgc tccataaata atcgcttgca ccgctgcaag ttaaagttac gctgcctccg
     8221 gcacaaaatg ttgtgggccc gtttgcggta attgttggag ctaccccacc ttgttttgtc
     8281 agtgtaaatg gcactgccat tacgttgttg ttatcattct cttcaagaaa ataattgtca
     8341 ggatcctgtt gacatacgat gtaataggta ccattacaag taccgggagg aatgttaatc
     8401 cacatgccat caagactctg attataggta tcaaaataac ctgctgaaat accttgcaat
     8461 gttgctgaac aaccataact tcctccacct aaaccatagt tgccataaaa ttctgcaggt
     8521 gtatccaatg tatcgccatt gtctgcaata cataagccac ttccgtttga tgatgcacag
     8581 gctcctatat caagcaaaca aaaagcaagt tttgcaccat tacctacaat aggccaattt
     8641 acaggattag gatctgctgt tggtgttctc aaagtataaa cgccccagtt gtcaacgtgt
     8701 tgatgaccat gtgatgggtg atatgtcatt gttcctgcag gacgatcata atatgacatt
     8761 gaattgccgt tcttatgata aattctttgg ttgatgatta ttttaggata ggtaatgtta
     8821 tcaggacaaa ttgatggcgc tgtaccaaca aaagtatctg tgccgcaaac aaaaatattt
     8881 gacgagcgtg tttccatagg tccatgtccg atgtttggtg tagaaactgt aattctcagg
     8941 cgtccatcat taacaccgtt gcctgactgc gaatattcta taacaccgtt ggaaccaagt
     9001 atggtcaatg gttttcttga tgcagtaata tcaggtaata aatcacagtt agtgccaccg
     9061 cccaagcatg agcatgttga ggcgttagtt agtgtacact gtgcataaat ggcttgacta
     9121 gcaaacaata gtacaaccag tagtaggtaa atacgtttca tattagtttg atttaaatat
     9181 tatgggagga ttaaaaagta gcccaaatta agcatttttc tgaaacttgc aagtggtttt
     9241 tttgattaac agaaaaaatc tgtagtcaaa aagcagaaaa atataggtga acagctattt
     9301 ttcggaaagc aaggtagtga tgatgcggat aaagtcatcc ttagtttttt catgataggc
     9361 acctgtagcc ggaccattaa atcctgtata gatttttcta agaataccaa acaggtagat
     9421 tttttttctt ccagtgcttt tatagcacct aaaaaggtgc tgcatttacc tccaccaccc
     9481 tcaaatgaca agtatttaat gttgcttttg ttcattataa tattgcttgt tttttttaag
     9541 aaaaaaagaa actgttagtg ttttaaaagc caccttatca taattttcag gatcataatc
     9601 gcctctatac agaaattttt ttaaattctg tgaatatcga atctccacac aaatatcatt
     9661 atcttcacaa ttgtgtggtt catcaagaaa tgggtatttt ggagaaaacc aaacatcaag
     9721 catggaatca atttcagcag cttttaaaac actggaatca aaatctaatg tcagaatgtt
     9781 tacaggaaac tttgcttcaa gtaatttata gtgttgcctg tacttattct cgtaaaattt
     9841 attgcttgca taatctgcgg agtagttatt aatagcagta tccatatccc gttgagcgta
     9901 atgtatccaa tatggcgccc attcatagta aatatatgct acaatacggg tacgggggtt
     9961 atattcttaa attacattta aaggaaattc gtatttttca gggcgatgaa aaattacgaa
    10021 cgctggtatt tctttaggac tcattataga atcataataa gtagttggta cattaaaatt
    10081 cgttttgaag ttgacccctt gtgcaagata actgatttta gaacctaact gtttttcgaa
    10141 tttgtaagca tcattaatat atttaatatt taaattaatt actaaattat ctttagagaa
    10201 gaaccatccg gttgtatcgg caaaaatagg aatataagat tcacttattg cctgatttga
    10261 attagaattg caggaactaa aaaaaacgat aagtgccgaa atgacaaggt agagtttgcg
    10321 catggtgtga tttgttgttg tcaatttata aattccaaac tttctaacac accaataatt
    10381 tctttataat ttttttcggt tttggcttta tttgaataat aaaaaatgga ctgcaaaaaa
    10441 aacaagtcgg atttgtgaag acaataagca ttaacaactt gaatattaaa ttctcctgat
    10501 gatttaaaaa atttttgctt tattaaaaaa tggttttttt tatcggattt gagcttagtt
    10561 cttaaagtat acttaattct tacttgaaaa gttggtgcat gttcatctaa actatcaatc
    10621 tctgctaaac tttttacagg tgagttgaat tcaaccccta cgattaaaga gttgcaatat
    10681 ttaataaact catcaatctt agagctgtct ccattaaaag ttgccgaaaa aaattcaaac
    10741 ttatccccaa ggatttcttt ttcgtatata ctatccacta tagaaaatct tttgggaata
    10801 taaattgaaa gggtgtttaa agagtcctta aaaagtatcc cactaatatt ttgaggttta
    10861 gaatcacttc tgcaatttgc caggaataaa attgaaacaa agagaaaaag gttattgaac
    10921 attatcgtag atttttttgc aagcattttc tataactttc tcctgctttt ctgcaagact
    10981 atttaagttt ctatattgac ggttgattaa cgggtttgag aaatcaagca attctaggtc
    11041 tgtaacatca attgaaacgg ataaattatt ctcaagaacg gacctaattt gtccttcttc
    11101 gcttggatat agaatggtat tgtaaagttg attaataaaa tcttttataa taacgctatt
    11161 tccttctttg aaatcatcag acacctcgta ttccttaact aaattttgca gacgaattcc
    11221 caaaatacag tcgcaaaatt ctgtagctga tgaactgata ttgggagaag ctaaaggata
    11281 gttatcaaaa ttgccttggt agttaatatt tttttgcggt ctgtaatcga aggcatgtaa
    11341 cgggtagttg ttgagcatac ctccatcaac atacagccca agataatctt tattataatc
    11401 agcagggaga tttgaatcga ggttaacttc tgactcgatt aagtaaggcc taaacataaa
    11461 aggtatgctc atggataaac taattgcatc aacaacagaa aaatcaggtg taaaagcggc
    11521 agagaaatat tttggtttgc ctaacgaaat attggttccg gtaacgataa gatcaacacc
    11581 agtcatgtaa aaaaaatcct gaaaagtgat atcctcacct ctggatttta gctttttcat
    11641 ttttagtgtg gaaagtttcg gaaataaata attttcaatt aaatcggaaa aatacttttt
    11701 agaggcgaca ccgcttagta atcccctatt ataaataata cctaaaaagt atttctttaa
    11761 atttaatgct ctagcaggag taccttcggt aagtaatatt tttcttacaa aaatttcatt
    11821 ctctgaaacg aaaggcttaa atgtccactt aaatacgttg aggaactcgg aaagtacatt
    11881 gcctataatt tttataggca ttgaaagatt attatactcc cttaatggcg attttttatt
    11941 atttgttcga taattatata aatccgggct atcaaaaaaa ctttcaaatt gtgttacgga
    12001 aacatctttt tgagcaatgg taaaattccc caacgataaa cttacaactt tgtttgtttc
    12061 ttgcagaatt tcatagtggg acatacccat cgcaaccata aaagcggtga ttgcaccagc
    12121 cgaagaacca ctaactccaa gaatatttct ttgtttgatt ggttgcgaaa ttctaataat
    12181 tggtgttttg ttttcagaag ttacgttata tggggtaccg ccttcaggtg gccccatttt
    12241 tttattaatt atttcttcta atgcttggat tacacctaaa taggcaagcc ctttaccgcc
    12301 acctccttca aagcacaaat atttaatttg tgaaatttct atcatagcaa gagtttttat
    12361 ggaagcaact catccgaaaa atcaggcagt ccggggcttg agccatcaag ttttgtgagg
    12421 taaacaagat tggtaggggc tttctgaatc caaggttcac cttcttgatt acctccaaca
    12481 aattgtccgt caaattcttt catttcgtca atgatagata tataaagcgg gtcggttggt
    12541 gagggtacgt cttcaccatt ccaaatttca ccacccgaac ttaagtaata caaaattgtg
    12601 actcgtccta aaaaaagttt acagtttaat tgattaatcc tgatataagt ttacaattca
    12661 tttttagtcc tgaattgggt ttacaatagt aggaattttt cgataataat tcttacataa
    12721 tttttctgtt ttgataattg tttttgaatg atgaataccc gtaggtgcga gcgtcctttc
    12781 cgctcgtgcc attcattttg tttgcatctt gcaaactagt aagatttact ttgccggtgt
    12841 atgagcgaaa aatacaaaat atttcctgac aagttatact ttgttacatt aaccacagtt
    12901 ggttagattg atgtttttac aagagccatt tactgcgatg agatagttaa gaacttaaat
    12961 tactgcattg acaataaaaa actaaaagtg tttgcttttt gcattatgcc atcacacatt
    13021 catttaattg caaaaacgaa agacgatgtg agtttaagcg atgtgttacg cgactttaag
    13081 agtaacacat caaagcaaat tattaaaatg attgaagaaa gccacacgga aagcaggcgc
    13141 gaatggttgc tttatatgtt tgagttttac ggcaaaaaaa tgcgcacaat gttaaatatc
    13201 agttttggca acagaataat catgccttcg atttattcag taataaattt attgatcaaa
    13261 aagtaaatta catacacaac aatcctgtag aaacacgaat agttacagag ccccattttt
    13321 atgtgtatag cagtgctaat caatttaccg atttaaagct ggttgaacta tgaagtccga
    13381 tagctatcgg acggccagaa gccacaagga taaaaggcac gagcggaaag gacgctcgcg
    13441 cctacttagt aatagcagga aactactcga aattaaattt agaatatttc gaaagttgaa
    13501 atattctaaa ttttgtgata tttctttata gtcacttgac ataatatctt ggagaatctg
    13561 cgaggataaa gggaataaaa aagtaactta aattaagcct ttttcctgaa acttgcaagt
    13621 ggtttttttg attaacagaa aaaatctgta gtcaaaaagc agaaaaatat aggtgaacag
    13681 ctatttttcg gaaagcaagg tagtgatgat gcggataaag tcatccttag tcctttcatg
    13741 ataggcgcct gtagccgggc cattaaaacc tgtatagatt ttcctgactt tgccctttcg
    13801 atcaataaaa atggtagtag gatagccatt gattttactg agcatactca ttttagatgc
    13861 agccccttct ctgttaccgg caatcagtgt ttcgtactga acatggtatt tttctttcac
    13921 tcgtttaaca ttaccgacag atttcctgaa gtcagatgtt ttttcaaaag ccaaaccaat
    13981 tatggttaag tcttggcttt tatttctgga ataataatcg gataaaaatg ctgtttcgtc
    14041 catacagtta gggcaccatg ttcccatcat ctcaacaaca accaccctac cttttaaatc
    14101 tttattaggg tatgaaaaaa ctgtgctgtc caatcgcaat gcactaaaag aaaaagatgc
    14161 agtatcattc ttcagaaaag tgagtttaaa cggatcgggc aattctgcac tttcattaac
    14221 tactgcttgc cagggttgct gccaatgatg tcctgaataa aaaacaccct gcatcatatt
    14281 attctttatt gagcctttaa aaagataagc aaatgctccg tcaaaataag ataaaaacaa
    14341 actgtcgtta ttaagacatc catcaagaaa acgatagtcg cccgactcag ttaaaagtgt
    14401 tcctctcaca tctgtaccat tctgtttaaa caatgcaatt gcttgcgatg aatctttagt
    14461 acccgggctg aatttccatt cccatttttc aggaagtttt atttcagatt ttgtgttatt
    14521 aaaaaaacga tacttttctc caagaaaagc tttgaatggt gtgggtgcat aattctttct
    14581 tgaattgtta taccaaaagc caatcatgct gtcaccgctt actctcactc tgaactcaga
    14641 atcataaacc ggtaattgaa taattaaaga atcattggca taagaaattt catttacttc
    14701 aatttcttca ttgccatttt ttataaaaat ctcactgcgg ctattaacct ctcttgtttc
    14761 aaaattaaaa ggtaattcac cgcccggtgt cattaatact gctctccata taccggaagc
    14821 aataatcaaa ccatgcaatg cagtactact caagtttaat tatatatagt tatttacttc
    14881 gcgaaattct tttgcgtggc tctttgtcct ttggcttcac ctttgatgta gccatcttca
    14941 aagcattcac ttccatctca ctcaaaaaac gccatttacc tcgtgcaata tccttcttgg
    15001 tcaagcctgc aaaaactacc cggtctaatt taataacatt gtaatccata gattcaaata
    15061 ttcgtcttac aatacggttt ttacctgagt gtatttctac accaatctgt tttttgtcat
    15121 taaatccacc atcgtattgt atgtcatcaa cagtagccat tccatcatcg agcattacac
    15181 cttctttaat tttttcttta tcggaagatt ttaaatttct gtccaactct acatgataaa
    15241 ttttctgcac ttcataacta ggatgtgtaa gtttttttgc cagatcacca tcattggtaa
    15301 aaagcaaaac accggttgta tttctgtcga gtcggccaac aggataaaca cgctctttac
    15361 aagcaccggc aataagatgc ataacagtac gtctgtcctg cggatcgtcc attgtggtga
    15421 tgtaatcttt tggtttattg agcaacagat acattggctt ttcatttctg atagtttcac
    15481 cattgtattt gataacatca tcctgcgaaa ccttggttcc cattgtagta acaatttcac
    15541 cattgacaga aacaaggcct gcagtaatca actcatctgc ttcgcgtctg ctacaaatac
    15601 cactgttggc aatataacgg tttaatcgaa caagaccatc agacaattgt tctgatgatt
    15661 ccttttcttt tttactatat tttctgttgt catttttctt gcgatacggt ttatcaaatc
    15721 ttcctgaact tctttctttt ctgtcgtaag aacgtttctc tcttgtatca ctatcgaaag
    15781 atttgctaaa tcgtcttggc ttatcttctc cttcaacatt atcatctctt cttttaaaac
    15841 tttttgactt gtcaaatttt cctgaaccac caaagtcact tcgtttttta aatcttggtt
    15901 tctcatcacc ttcaaaacct tcatctcttt ttctgtaagg acgtggacta tctgatgatt
    15961 taaaacttct tttctctcgc tcaaaaccac cttctt
//
LOCUS       DAOOOL010000052        15840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_310_length_15840_cov_60.356414, whole genome shotgun sequence.
ACCESSION   DAOOOL010000052 DAOOOL010000000
VERSION     DAOOOL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_15840_cov_60.356414"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKU82_05895"
     CDS             complement(<1..522)
                     /locus_tag="PKU82_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82441.1"
                     /translation="MKTTKQVLITFDYELFLGNRSGSVSDCLINPTNHVLDVIEQYGC
                     RAIFFVDTTYLLTLKKNFQIAACKKDFETVMNHITEIASRGHEIFPHLHPHWLDAKYL
                     SATNEWQLLNTDKYRLYHINESERSEIFDQSIEILQQRISKVNPSYLVDSYRAGGWCI
                     QPFNTFLPYFIKHN"
     gene            complement(544..1716)
                     /locus_tag="PKU82_05900"
     CDS             complement(544..1716)
                     /locus_tag="PKU82_05900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82442.1"
                     /translation="MHILIIPSWYKSITEPVLGTFFEEQARALMSAGHKVGIIYPQFS
                     SVSSMFNKKDEIIDFYDDNGLPTYSVIHQAIIPKMRKLAYKMFGENVEKIYHSYVKKY
                     GKPDLIHAHSVFHGGMAGYYIAKKNKLPFIITEHLTAYITGGITHQEDVETARQIFEN
                     ANAAIIVSENFKNDIEKALNLPKTTFTVIHNMVADLFFKDIVLKKYNQQEEFVFFTNS
                     FLLPRKNHKLLLDSFKYITAKGINNLRLRIGGDGPLYNELRDYVNELSLTGKVDFLGA
                     LSRTEVKRELDNCHSFVLSSNYETFGVVLIESLACGRPVVTTESGGPSDFIHSRNGIT
                     SSTSIDSLANAMTKMMEEYNTFDQRALSEECRDRFAEKKIEAEIEALYLTAINKHK"
     gene            complement(1723..2676)
                     /locus_tag="PKU82_05905"
     CDS             complement(1723..2676)
                     /locus_tag="PKU82_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOZ82443.1"
                     /translation="MNNDAPFQFLPGFTHLYHEYMEENIFIAYSEKLMAYMPLRFFSS
                     RFFKLAQILHAPIKNNIELNPDEQLEFFNELISHLNQNNSCERLVQPHPYGILASVPA
                     NSRFCEFGTYIIDLQSQTLEEIFQKFHPKYQKAIHHSEKNGAVVKFGQEVIEDFYLCY
                     TDTMKRINMPSEELQFFKSYYNYLGSDNVTAGVVYDHDNPIGGIFMIHSNYAALCTHA
                     GSRGESKLYGSMKYLHSKMIERMKETGVNKYDLVGVRIGNNDPTLEGVFRFKRGFGGE
                     LKKGFLWKTDINPLKAKMYDLLIKIKHPNNKYKDIIDQITN"
     gene            complement(2731..3858)
                     /gene="wecB"
                     /locus_tag="PKU82_05910"
     CDS             complement(2731..3858)
                     /gene="wecB"
                     /locus_tag="PKU82_05910"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOZ82444.1"
                     /translation="MKKILIVVGTRPNFIKVTQFKKVNADLGMPFDIKIVHTGQHYDS
                     KMADIFFEQFNLTPDFWLNIPAGSPNSQMAEIMLRLEKVIQENKPDLLMVVGDVNSTF
                     AAALTANKMDLKIAHVESGLRSFDRTMPEEINRLLTDEITDYFFITEDSGYKNLLKEG
                     KHEKQMFFVGNTMIDTLVAFDEKIQQSNILETLNIKSKEFVLMTMHRPATVDHKDGLK
                     QLFSIIELITKNHQLVFPIHPRTLHRIEEFGMKSFITENKKLILTEPLDYLAFQKLIS
                     DCAYIVTDSGGIQEESTFRQIPCLTLRANTERPCTVDIGTNELVPFSQEKISERIDAI
                     VHNKYKKGVIPPLWDGQSTKRILEACMKFLSSESHASALLK"
     gene            complement(3913..5055)
                     /locus_tag="PKU82_05915"
     CDS             complement(3913..5055)
                     /locus_tag="PKU82_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOZ82445.1"
                     /translation="MIKRLISHIRLRYLNLLSQSTAENFSDEQRLLMALEWLKQTLLP
                     NGGSAAKYSMMFNKWLPAYPETTAFWINTLIYLKNNKPELLQEVFNDRPVIDELIQWL
                     LSTQRKDGTFPGSYGDFKNQPPRVFNNGQIIMALTDYYQQSQKKEVLEAAIQSADWLL
                     KVQDSDGAWREFTLHQLSSNTRTAWALIKLGQHLNEPKYISAGINNINFALKQINNHD
                     YFCDNGFDENEIPTTHTIGYALRGVLGAGIDLQKKEWTEIVERSYKHIVALTKQNGFL
                     AGELDEEWTSDNAFCCLTGNCQLSVIGFVLFEQTAEIQYLETANLLLEYVKSKQLISH
                     SPMVNGGISGSWPVNGGYSAYDIPNWAAKFFADAMLLQMKHKTMKH"
     gene            complement(5065..6858)
                     /locus_tag="PKU82_05920"
     CDS             complement(5065..6858)
                     /locus_tag="PKU82_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ82446.1"
                     /translation="MSPSKNNYSTAQAFRQTYKSLQDHLSPLQKKRIKWIAFLIFLSA
                     ILDVFGLASVLPLVKMATEPEIIHTNQYLSTAYSYLNFETDKSFMVFCIVIILLFQFF
                     KTGFGIFVNYIEGRFMADVANNISKNQFSKYFSLSYFNFNSVKSSRIINHVQQNPASF
                     TAWVMLPILMLFSESVILLCIVTLIAVYNLKLFLFILMIIGPASYILYKSVNTKNEQI
                     GRGLDKMFPQALAAINNSIMGFVDIKLANKIEYYRNRFLKYQKAYHDLSMNSIVINMI
                     PFRGYELVAILGIVVIFIYAIFIEGGQQDVIMMVGAFAAAAYRLMPSLNRIMNAVMQV
                     TKNQVAIENLNEYNELYVKQKEMDRNVPIAFENTIEFQNISFTFPKSHEPVLKSISFK
                     VKKGEKIGFVGSSGSGKTTLMNILLRFFDENSGQILVDGKPLKEENLEYWRGLIGYVK
                     QDIFMIDSNIKENVAFGEETINNDLLNAAIKQASLNEFVGNLPKGVESEVGERGSRLS
                     GGQKQRIGIARSLYRNAQILVFDEATSALDTQTEREVSEAIDSLSDTNKTIFIIAHRV
                     TTLKNCDRIYELKNGEIAGVYSYSELIEKVI"
     gene            complement(6873..9182)
                     /locus_tag="PKU82_05925"
     CDS             complement(6873..9182)
                     /locus_tag="PKU82_05925"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOZ82447.1"
                     /translation="MNNQKVIKNQSIIDAKDVKNALNLFVKNWFWFILFLSIGVAGGV
                     FLYYKTTRIYGAEAKVLITPTKNAFKDALSTSLPLGPNKEEVSNEIQILSSTKLVSEA
                     IKKLNLDISYYIKGRLKTGEVYRGTPFSVDGKILDDNFYGIPFNLRIIDKDSYSLDIK
                     TNDYSYNKVLKFGDPVVTDKFSIVINGKQESILKNSKFSEFQYVFVINNHSYLVKKYK
                     SALSLEKDEDASVININIADEVEEKAVDFLDTLTNLYINYSISVARGINENSLKFIEA
                     QLKDYEDQLNGVETSLVEYQQQSGAIDVANQQSTFLKEKIDVQGEKAKLTARLQSVEY
                     VYNQLTSGSSDFSTLSPALMTEQSNPGLTSAFNELSSLMQRKVNMSFSMTPNSPQIKD
                     VDVLINKAKENVIGIIMNIRKDIAMQINLLSSREGSFAASLNRMPSTIKGLSDITRKK
                     EINEKMYLFLLESRAQNVIASAAIVPDKSILEPASSTGLLRPIRNKMLFMGAGVGLAL
                     AFALVFLKSIFLNYIFTKEELADITNQPIIGVIGKSAEAKKDYLVVHEHPQSLAAEAF
                     RVIRTNLSYFAPKTSSKVILFTSTISGEGKTFCAVNTATMLARAKKKVVVVDLDLHKP
                     KQAVAWNMTNDVGVTSYIVGKATLKDTLKDTPVENLKVILSGPKSPNASELILDSMME
                     QLINELKEHFDYIILDMPPVGLLSDALVMMKHSDLNLYVMKAGYSKKDFVEVAHQLME
                     KNQIKSLSFILNGVNPKNMPVGYGGAYYK"
     gene            9381..10496
                     /gene="gmd"
                     /locus_tag="PKU82_05930"
     CDS             9381..10496
                     /gene="gmd"
                     /locus_tag="PKU82_05930"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ82448.1"
                     /translation="MKRALITGVTGQDGAYLSELLLQKGYEVHGIKRRSSLFNTERID
                     HLYEDMHQKNVKFKLHYGDLTDSANIIRIIQEVQPDEIYNLGAMSHVRVSFDMPEYTA
                     NADGIGTLRILEAVRMLNMTEKCKIYQASTSELYGLVQEVPQRESTPFYPRSPYAVAK
                     LYAYWIIVNYREAYNMFASNGILFNHESPLRGETFVTRKITRAVSRIALGLQDTVYMG
                     NIDAKRDWGHAKDYVEGMWLMLQQEKADDFVLATGVTTTVRDFIVLAFKEVGIEIEFS
                     GKAENEIAVVKKCNNPAYQVPVGNVVMKIDPKYYRPSEVELLIGDPAKAKQVLGWEPK
                     HNLASIVKEMMNCDLELFKRDQYLKQGGHKIINYHEA"
     gene            10501..11442
                     /locus_tag="PKU82_05935"
     CDS             10501..11442
                     /locus_tag="PKU82_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOZ82449.1"
                     /translation="MQKNSKIFVAGHRGMVGSAIVRKLQSEGFNSILFRTSKELDLRN
                     QRDVADFFNYEKPEYVFLAAAKVGGIVANNTYRADFIYENLIIECNVIKSAFDSGVKK
                     LLFLGSSCIYPKMAPQPLKEEYLFTGPLEPTNEPYALAKIAGLKMCEAFRDQYGVDYI
                     SAMPTNLYGPNDNYDLKNSHVLPALVRKFYDALANNLPSVEIWGTGSPRREFLHVDDL
                     ADACFFLMQNYSDRSTVNIGTGEDISIKELAMLIKETFGYQGQLNFDTTKPDGTPRKL
                     LSVDKLHQLGWKHSITLEQGVASVCNEVKSMESFLER"
     gene            11565..12431
                     /locus_tag="PKU82_05940"
     CDS             11565..12431
                     /locus_tag="PKU82_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82450.1"
                     /translation="MTKIYLKVFLFFLASLCSLGSLNAQTQTVVVGTSSLISAFSPIV
                     KSYECVAYEAIYLSTEIGYSGSISSFGFNRVDGTDTLPFDSVSIFMKQTAFTSLTAGN
                     VDTTGYTLVYEGPFPNNGGIGWREVALDTSFTYSTSSNLSVMVFKHTQTATLSTPVSP
                     RWYYTSVSSNRSRRYYGAIPISSVTSLSATNVLSDIRITVGTVGIVDISAGLFSVYPN
                     PTNGIIHIVLHNSEISSLKISDINGRIVKEMYNLKDGSLDLTNLKDGVYNLQFFDKSK
                     NNIATRIIVVKR"
     gene            complement(12470..14227)
                     /locus_tag="PKU82_05945"
     CDS             complement(12470..14227)
                     /locus_tag="PKU82_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOZ82451.1"
                     /translation="MLHNNLNMQNALKFILAYLFFTGNVSAQEILPNYMTQKEMLQMP
                     SYLQQLSNQLKNNQIAAPPSSPVRTIAEWEELQGILIGWTSYPSILREIVRAAKTECR
                     VYVVTNNEQSVINYLNSGNVDTINVSFVNTPFNSVWSRDYGPWSAYTNDVDTLVTIDW
                     IYNRPRPDDDNVPVAIAGLLNTPLYQTTMSPYSLVHTGGNFMCDGFGTGFSSNLIVNE
                     NPSLTISQIDTIMKQFMGINRYIKMPTLPYDAIHHIDMHMKLLNEETILMGQYPQGVA
                     DGPQIEANLLYVINNFNSVFGTPYKIIRIPMPADNGAYPNTNGDYFTYTNSSFINNTI
                     IVPTYNIPQDSTALRIYRDALPGYNVIGINSLQSIGAIGALHCITKDLGTNDPLLISH
                     QQLSNTYDTINPYHVEAFIKHRSGIQSATLYYRTDTLQPYNTVSMNAISGMSDYFETY
                     IPAQSGGTRIYYYIEAYSNTSKHQTRPMPAPQGYFYFDVLQTTGITEVLNQLSVGQIF
                     PNPASAVTCLPYYSRIGTLLQIKISDVTGKMVYESQNTTKSNGQNFAFFDVSQFKPGV
                     YFVKSETNSSINIQKLIVK"
     gene            14312..15163
                     /locus_tag="PKU82_05950"
     CDS             14312..15163
                     /locus_tag="PKU82_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOZ82452.1"
                     /translation="MKWMGRRESDNVEDRRRFSGGKIALGGGLVGIIVIVINLLMGGD
                     PNQLLQQMQQQATTQTTTTESSAADDEMASFIKVALADNEDIWKKIFQENELEYREPK
                     LVLFRDATQSGCGNASSAVGPFYCPADEKVYIDLSFFDELKSRFGAEGGDFAVAYVLA
                     HEVGHHVQHLLGTSDKVHQMQEGLSETEANKLSVALELQADFYAGVWANQNQKLNNVL
                     EDGDIEEALSAASAVGDDKIQKKAQGYVVPDAFTHGTSEQRMYWFKKGYQTGKLQEGN
                     TFKELNI"
     gene            15269..>15840
                     /locus_tag="PKU82_05955"
     CDS             15269..>15840
                     /locus_tag="PKU82_05955"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HOZ82453.1"
                     /translation="MQKFTSSLMALIIATTTTSMAQLKTPQPSPTQSLTQAFGLGEIK
                     IEYSRPSVKGRTIFGDLVPYGKIWRTGANQSTKVTFGADTKVNGNDIAAGTYALYTIP
                     NTTDWEIIFYKDLTLGGNVGDYKQTDEMMRFKVKPVATADKMETFTININNIAPTSCS
                     IDLLWDKTKVSIPVTTNIDENVMKQIDKAMG"
BASE COUNT         4989 a         2713 c         2823 g         5315 t
ORIGIN      
        1 attatgttta ataaaatacg gtaaaaaagt attgaatggt tgaatgcacc atccgcctgc
       61 acgataacta tctactaaat aagatggatt aacctttgaa atacgctgtt gaagtatttc
      121 gatagattga tcaaaaattt cagaacgctc agattcgttt atatgatata agcggtattt
      181 atcagtgttt aacaattgcc actcattggt agctgaaaga tattttgcat ctagccagtg
      241 tgggtgtagg tgaggaaata tttcatgacc acgacttgca atttccgtaa tgtgattcat
      301 tacagtttca aaatctttct tacaagctgc aatttgaaaa ttcttcttta gtgtaagaag
      361 ataagttgtg tcaacaaaaa aaatggctct gcatccatat tgctcaatta catcaagtac
      421 gtgattggtt ggattaataa gacaatctga aaccgatccg cttctgttgc ctaaaaataa
      481 ctcataatca aaagtaatta atacctgctt ggtggttttc atatttttaa gatcagaatt
      541 actttactta tgtttattaa tagctgtcag ataaagtgct tctatttctg cttcaatctt
      601 tttttctgca aatctgtctc gacattcttc agataatgca cgttgatcaa aagtattgta
      661 ttcttccatc atttttgtca ttgcatttgc aagactatca attgatgttg atgaggtgat
      721 tccatttctg ctatgtataa aatcactcgg tccgcctgat tctgttgtta ccaccggtct
      781 tccacaagct aaactctcta tgagtacaac accaaatgtc tcatagttac ttgaaagcac
      841 aaacgaatgg caattgtcta attctctttt tacttcagtc cgtgataatg cacctaaaaa
      901 atctactttg cctgttagtg ataactcatt tacataatct cttaattcat tatacaatgg
      961 gccatcacca cctatccgta accgtaaatt attaattcct ttagctgtta tgtatttaaa
     1021 tgagtcaagt aataatttgt gattctttct tggcaatagg aatgagttgg taaagaaaac
     1081 aaattcttcc tgttgattat atttttttaa aacaatgtct ttaaaaaaca aatcagctac
     1141 catattgtgt ataaccgtaa aagttgtttt tggaagattt aaggcttttt caatatcatt
     1201 tttaaaattt tcacttacaa taattgctgc atttgcattt tcaaaaattt gtctggcagt
     1261 ttcgacatct tcctgatgag tgattcctcc tgtaatgtaa gctgtcagat gttctgttat
     1321 aataaatgga agtttattct tctttgcgat ataatagccg gccattccac catggaaaac
     1381 actgtgtgca tgaattaaat ctggtttacc atatttctta acataactat gataaatttt
     1441 ctcaacattt tctccaaaca ttttataagc tagctttctc atcttaggta tgatagcctg
     1501 atgaataaca gaataagtcg gaagcccatt gtcgtcataa aaatcaatga tttcatcttt
     1561 cttgttaaac attgaactta ctgatgaaaa ttgtggatat atgattccaa ccttatgtcc
     1621 tgcagacatg agtgcacgcg cctgttcttc gaagaatgtt cctaaaacag gttctgtgat
     1681 agatttatac caagaaggta taattaaaat gtgcataaaa aattaatttg taatctgatc
     1741 aataatgtct ttatacttgt tatttggatg tttgatttta ataagcaaat catacatttt
     1801 tgcttttagc ggattaatgt ctgtcttcca taaaaaacct ttctttagct caccaccaaa
     1861 tcctctttta aagcgaaaaa ctccttcaag tgtagggtcg ttgtttccaa ttcttacgcc
     1921 tacaaggtcg tatttattca ctcctgtttc tttcatgcgt tcaatcatct tactgtgtaa
     1981 atatttcatt gaaccatata gtttggactc gcctctgctt cctgcatgtg tgcataatgc
     2041 tgcataattt gagtgaatca taaaaatacc tcctattgga ttgtcatgat catatacaac
     2101 acctgctgta acattatcgc tgccaagata attataataa gatttaaaaa actgtagctc
     2161 ttctgaaggc atgtttattc gtttcatagt atcagtataa cataaataaa aatcttcaat
     2221 cacttcttgt ccgaatttta caacagctcc atttttttct gaatgatgaa tagctttttg
     2281 atattttgga tgaaacttct gaaagatttc ctccaatgtt tgtgattgaa ggtctatgat
     2341 ataggtgcca aattcacaaa aacgcgaatt ggcaggtaca gaggcaagaa ttccataagg
     2401 atggggttga accaatcgtt cgcaggaatt gttttgattt aagtgcgata ttaattcgtt
     2461 aaaaaattca agttgttcat ccggattaag ttcaatatta tttttaattg gcgcatgtaa
     2521 tatttgtgca agtttgaaaa atcgtgaaga gaaaaatcgc aatggcatat atgccatcaa
     2581 tttttctgag tatgcaataa aaatattttc ttccatatac tcatgataca aatgagtaaa
     2641 cccgggaaga aactgaaacg gtgcgtcatt attcattgcg gccaataatg acttgtcttt
     2701 tttctgtagg tggttgagta gtaacatagt ttatttcaat agggcagagg catgactttc
     2761 gcttgaaaga aatttcatac aagcttctaa aattcgtttg gtggattgtc catcccataa
     2821 tggaggaata actccttttt tatatttgtt atgtacaatt gcatcaatgc gttcggaaat
     2881 tttttcctga ctgaatggta ccaattcatt agttccgata tcaactgtac atggtcgttc
     2941 tgtattggct cttaatgtaa gacatggtat ttgtctgaat gtactttctt cctgaatacc
     3001 tccgctgtct gttacaatat aagcacagtc tgaaataagt ttctgaaatg ccagatagtc
     3061 taaaggctct gttaatatta gttttttgtt ttccgtgata aatgatttca ttccaaactc
     3121 ttcgattctg tgcagtgtac gtgggtgaat cggaaataca agttgatgat tttttgttat
     3181 aagttcaatt attgaaaaca attgcttcag accatcttta tgatcaaccg ttgccggacg
     3241 atgcatagtc atcaggacaa actctttact tttaatattt aaagtttcaa gaatattact
     3301 ttgttgaatt ttttcatcaa aggctacaag tgtatctatc atggtgtttc caacaaagaa
     3361 catttgcttt tcatgcttgc cttccttgag aagattttta tagccactgt cttcggtaat
     3421 aaaaaagtaa tcggttatct catcagttaa taaacggttt atttcttcag gcattgttct
     3481 gtcaaaactt ctcaggccac tttcaacgtg agcaattttt aaatccattt tattggctgt
     3541 taatgctgca gcaaaagttg aatttacatc gccaacaacc attaataagt caggtttgtt
     3601 ttcctgaata actttctcta agcgaagcat tatctcagcc atttgagaat taggactgcc
     3661 tgccggaatg ttaagccaga agtcaggtgt taaattaaat tgctcaaaga aaatgtcagc
     3721 catcttacta tcgtagtgct ggccagtatg aactatttta atatcgaaag gcatacctaa
     3781 gtcggcattc acctttttaa actgtgttac tttaataaaa tttggtctgg tcccaactac
     3841 aattaaaatc tttttcattc tcaaaatcta ttgtcttttt atacgtgaag gtaggtataa
     3901 agtttaaaat atctaatgtt tcatagtttt atgtttcatt tgcaagagca tggcatcagc
     3961 gaaaaatttt gctgcccaat ttggtatatc ataagcagaa taaccaccat ttacaggcca
     4021 tgatcctgaa atgccgccat ttaccattgg cgaatgtgaa attaattgtt tgcttttaac
     4081 gtattcaagc aaaagattcg ctgtttctaa gtattgaatt tcggctgttt gttcaaataa
     4141 tacaaatccg ataacactta actgacagtt gcctgtcaga cagcagaatg cattgtcgct
     4201 tgtccattct tcgtctaatt ctccagccag aaagccattt tgtttagtaa gtgcaacgat
     4261 gtgtttatag gagcgctcaa caatttcagt ccattctttc ttttgaagat caatacctgc
     4321 acctaataca cctcgcaatg cataaccgat agtgtgtgtg gttggtattt cattttcatc
     4381 aaaaccattg tcacaaaagt agtcgtgatt attaatctgt tttaaggcga agttgatatt
     4441 attaattcct gctgaaatat attttggttc atttaaatgt tggccaagtt tgattaaagc
     4501 ccatgctgtt ctggtgtttg agctaagttg atgtagcgta aattcacgcc atgcaccatc
     4561 agaatcttgc actttcaaaa gccaatcagc actttgaata gcagcctcaa ggacttcctt
     4621 tttctgactt tgttgataat agtcagtaag cgccataatt atttgaccat tattaaaaac
     4681 tctgggaggt tggtttttaa aatctccata cgatccggga aatgtaccgt ctttacgttg
     4741 agtagataaa agccattgaa ttaactcatc aataacaggt ctatcattaa atacttcctg
     4801 taataattcc ggtttgttat ttttcagata gataagagtg tttatccaaa aagcagtggt
     4861 ttcagggtag gcgggtaacc atttgttaaa catcattgaa tacttggcgg cacttcctcc
     4921 atttggtaaa agtgtttgct tgagccattc aagtgccata agtaatcttt gttcatcaga
     4981 aaaattttcg gctgttgatt gcgataataa attgagatat cgcaacctta tgtgtgaaat
     5041 cagccgtttg atcatcgtta aaaattaaat tactttttca attaactcgc tgtaggaata
     5101 tactcctgca atttccccat tttttagttc gtaaatgcgg tcgcagtttt ttagtgtagt
     5161 cacgcggtga gcgatgataa agattgtttt attggtatct gaaagtgagt caatggcctc
     5221 acttacttcg cgttctgttt gtgtgtccaa tgcactggtt gcctcatcaa aaacaagtat
     5281 ctgtgcgttt ctataaagcg agcgtgcaat gccaatcctt tgtttttgcc caccagatag
     5341 tcttgatcct ctttctccaa cttctgactc tactcctttg ggtaggttcc caacaaactc
     5401 atttaatgat gcttgtttga ttgcagcatt aagtaaatcg ttattgattg tttcttctcc
     5461 aaaagcaacg ttttccttta tgtttgaatc aatcataaaa atatcttgct tcacatagcc
     5521 gattaacccg cgccagtatt caagattttc ttcttttaat ggtttgccgt caactaaaat
     5581 ctgccctgaa ttttcatcaa aaaaacgtaa taaaatattc ataagagttg ttttgccgga
     5641 acccgaactg ccgacaaaac caattttttc tccttttttt actttaaatg aaatgctttt
     5701 taaaacaggt tcatgcgatt ttggaaacgt aaaactgatg ttctgaaatt caattgtgtt
     5761 ttcaaaagca ataggcacat ttctgtccat ttctttttgc tttacataaa gctcattata
     5821 ttcatttagg ttttctatgg caacttggtt tttagttacc tgcattactg cattcattat
     5881 tctgtttaaa gagggcatta aacgatatgc tgctgcagca aatgcgccta ccatcatgat
     5941 cacatcctgt tgcccgcctt caatgaaaat tgcatagata aaaatgacaa cgattcctag
     6001 tattgcaacc aattcataac ctctgaatgg tatcatgttt atcacaattg aattcatgga
     6061 taagtcgtga taagcctttt gatatttcaa aaaacgattt ctataatatt caattttatt
     6121 agccagttta atatctacaa aacccataat tgaattgttg attgcagcaa gagcctgcgg
     6181 aaacatttta tccaatcctc tgcctatctg ttcatttttt gtgttaaccg atttatagag
     6241 tatgtatgat gccgggccga taatcattaa aataaaaaga aagagcttga ggttataaac
     6301 agcaatcagt gtgacaatgc aaagaaggat tactgactct gaaaatagca tcaggatggg
     6361 taacatgacc caggctgtaa atgaagctgg gttttgttgt acgtgattta taattcgtga
     6421 tgatttgacg ctgttaaaat taaaatagct taaagagaaa tatttcgaaa actgattttt
     6481 agaaatgttg tttgcaacat ctgccataaa ccttccttca atatagttta cgaatatgcc
     6541 aaatcccgtt ttgaaaaatt gaaataacaa gataataaca atacaaaata ccatgaaaga
     6601 tttatctgtt tcaaaattca gataactata tgctgtactt aaatattggt tggtgtgaat
     6661 aatttcaggt tcagtagcca tttttactaa tggaagaaca gaagctaaac caaacacatc
     6721 taagattgca gatagaaaaa tcagaaatgc aatccattta atccgttttt tttgcagtgg
     6781 agataaatgg tcttgaagac ttttataagt ttgcctgaag gcttgagctg tagagtagtt
     6841 gtttttgctg ggcgacatac tttttgttcg tgttatttat aatatgcacc accataccca
     6901 acaggcatgt ttttcgggtt tacaccattt aagataaagc tcaggctctt gatttgattc
     6961 ttctccatta actgatgagc aacttcaaca aagtctttct tgctgtatcc ggctttcatg
     7021 acatatagat ttaggtcgga atgtttcatc atcactaaag catctgacaa caatcctacc
     7081 ggtggcatat ctaaaatgat gtaatcgaaa tgttctttca attcattaat taattgttcc
     7141 atcatcgaat ctaaaatcaa ctcagaagca tttggtgatt ttggtccgct taaaataact
     7201 tttagatttt caacaggagt gtcctttaaa gtatctttta aggtagcttt accaacaata
     7261 taagatgtta cacctacatc gttagtcatg ttccatgcaa cagcttgttt gggtttgtgt
     7321 aaatccaaat caactaccac aactttcttt ttagctcttg ctaacatggt cgctgtattc
     7381 actgcacaaa aagtttttcc ttctccggaa atagtagaag tgaataaaat aactttcgaa
     7441 cttgttttag gtgcgaaata ggataagttg gtgcgaatta ctctgaaagc ttctgctgca
     7501 agtgattgtg gatgttcatg cacaacaaga taatctttct tggcttctgc actttttcct
     7561 ataacaccga taatcggttg attggtaata tcggcaagtt cctctttagt aaaaatataa
     7621 ttaaggaaaa tactttttag aaagactaaa gcaaatgcaa gtgccagacc cactcctgct
     7681 cccataaata acattttgtt tcgaatggga cgtagcaatc ctgtacttga tgcgggctca
     7741 agaatagatt tgtcaggtac aatggctgcc gatgcaatta cattctgtgc cctgctttct
     7801 aataagaaca aatacatctt ttcattaatc tctttttttc ttgtaatgtc actaaggcct
     7861 ttaatggttg agggcattct gtttaatgat gctgcaaaac taccttcacg acttgacaat
     7921 aagttaattt gcatggctat atccttacgg atgttcatga taataccaat aacattttct
     7981 tttgctttat taattaaaac atctacatcc tttatctgcg gactgttggg tgtcatgcta
     8041 aatgacatgt ttacctttcg ttgcatcaag gaacttaatt cgttaaatgc tgaagtcagt
     8101 ccgggattag attgttctgt cattaaagcc ggtgaaagcg tactgaagtc actactgcca
     8161 cttgttaatt gattataaac atattctaca gattgtaatc gtgctgtaag tttagctttc
     8221 tcgccttgca catcaatttt ttcctttaaa aaagtacttt gttgattggc tacatcaatt
     8281 gcgccacttt gctgttggta ttcaaccaag cttgtttcaa caccattaag ttgatcttcg
     8341 tagtctttta attgtgcttc aataaatttt aatgaatttt cattaattcc tcttgctact
     8401 gaaattgaat aattgatata aagatttgtt aacgtatcca gaaagtcaac agctttttct
     8461 tctacctcgt cagcaatatt gatatttatt acagatgcat cttcgtcttt ctctaaactg
     8521 agggcacttt tgtatttctt aactaaataa ctgtgattat taattacaaa tacatattga
     8581 aactcactga attttgaatt tttaagaata gactcctgct taccattaat gacaattgaa
     8641 aatttatccg taaccacagg gtcaccgaat ttgagcactt tattgtatga ataatcattg
     8701 gtttttatat caaggctgta tgagtctttg tcaataatgc gaagattaaa tggaatgcca
     8761 taaaaattat catctaatat tttaccatca acactaaaag gagttcctct ataaacttca
     8821 cctgttttta aacgtccctt tatataatac gaaatgtcaa gattaagctt tttgatagcc
     8881 tcagaaacta attttgtaga tgacagtatt tggatttcgt tggaaacctc ctctttattt
     8941 ggtcctaatg gaaggctcgt tgataatgca tctttaaagg cgtttttggt aggtgtaatc
     9001 agcaccttag cctctgcacc atatattcgg gtagttttat agtacaagaa aacacctccg
     9061 gcaacaccga ttgacaaaaa caaaataaac cagaaccagt tctttacgaa aagatttaag
     9121 gcgtttttga cgtccttagc atcaataatg gattggtttt taataacttt ttgattattc
     9181 ataaaatcaa taatttactg aacacactta tacttaaaac ctgtataaat ggttcgctaa
     9241 ttggtggttt aaaattgttt aatttgcaca aaattaaaaa attaaaacgg gttataattc
     9301 gtgtttttta attttattaa ctaattgtag taactaagta gaaccacatt acgtttacca
     9361 tattttaatt ttaatcatca atgaaaagag cattaattac cggtgttacc gggcaggatg
     9421 gcgcctacct aagcgagtta ctgctgcaaa aaggctatga ggtacatggc attaaacgca
     9481 gaagttcact ttttaataca gaaaggattg accatcttta tgaagatatg catcaaaaga
     9541 atgttaaatt caagctgcat tatggcgatt tgacagattc tgccaatatc atcagaatca
     9601 ttcaggaagt acaacctgat gaaatttata accttggagc aatgagccat gtgcgtgtaa
     9661 gtttcgatat gcctgaatat actgccaatg ccgatggtat cggaacacta cgtattctgg
     9721 aagctgtacg aatgttgaat atgactgaaa aatgcaaaat ctatcaggca tcaacaagtg
     9781 agttgtatgg tttagtgcaa gaagttccac agcgagagtc cacaccattt tacccgcgtt
     9841 cgccctatgc ggtagcaaaa ttgtatgcct attggataat tgttaactat cgtgaagcat
     9901 ataatatgtt tgccagtaat gggatattat ttaaccatga atcgccatta agaggagaaa
     9961 cctttgtgac ccgtaaaatc acacgagcag tatctcgtat agccttaggg cttcaggaca
    10021 ctgtttatat gggaaatatt gatgccaaac gcgactgggg ccatgcaaaa gattatgtgg
    10081 aaggtatgtg gttgatgttg caacaggaaa aagccgatga ctttgtgttg gcaaccggtg
    10141 ttacaacaac agttcgcgat tttattgtac ttgcatttaa agaagtagga atagaaatag
    10201 agtttagtgg aaaggctgag aatgaaattg cagtagttaa aaaatgtaac aatcctgcat
    10261 atcaggttcc ggtgggcaat gtagttatga aaatagaccc caaatattac agaccctctg
    10321 aagttgaatt gctgattggc gaccctgcaa aagctaaaca agttttagga tgggagccta
    10381 aacacaacct ggcatcaata gtaaaagaaa tgatgaattg tgaccttgag ttattcaagc
    10441 gcgatcaata tttgaaacaa ggcgggcata aaattattaa ttatcacgaa gcataatata
    10501 atgcagaaaa acagtaagat ttttgtagca ggtcaccgtg gaatggtagg gtctgcaata
    10561 gttcgtaaac tgcaatccga aggatttaat agtattcttt ttagaacatc aaaagagctt
    10621 gaccttcgca atcaacgtga cgttgctgac ttttttaatt atgaaaaacc ggaatatgtt
    10681 tttcttgctg ctgcaaaggt tggtggcata gttgccaata atacctatag agcagatttt
    10741 atttacgaaa atctgattat tgaatgtaat gttattaaaa gtgcatttga ttccggagta
    10801 aaaaagttgt tgtttttagg ctcatcatgt atttacccta aaatggcacc acagccatta
    10861 aaggaagagt atttattcac cggtccgtta gaaccaacaa atgaacctta tgcattggcc
    10921 aaaatagcag ggctaaaaat gtgcgaagcg tttcgcgatc aatatggagt agattatata
    10981 tcggcaatgc caacaaatct atatgggcct aatgataatt acgacttaaa aaattcacat
    11041 gttttacctg cattggtccg aaagttttat gatgcattag cgaacaatct gccgtctgta
    11101 gaaatttggg gaacaggatc tccaagacgc gaatttttgc atgttgatga cttggcggat
    11161 gcatgttttt ttctgatgca aaattacagt gaccgttcta cagtaaatat tggtacaggc
    11221 gaagatatca gcattaaaga attagcaatg cttattaaag aaacattcgg ttatcaaggc
    11281 caattgaatt ttgataccac aaaacctgat ggtacacctc gcaaactttt gtcggtagat
    11341 aaattgcacc aattaggttg gaagcacagc attacattag aacaaggtgt tgcaagtgta
    11401 tgcaatgaag taaaaagtat ggagtctttc ttagagcgct aaaaatgcat ttgacaaaat
    11461 ataagtttta aatactttag aagacactct cataattttc aaaataaaag tttaaaacat
    11521 caatcaattt gatgtatatt cgtgaaatac taaattattt tacgatgaca aaaatctact
    11581 taaaagtttt tttattcttt ttggcttcat tatgttcatt gggcagtttg aatgctcaga
    11641 cacaaacagt tgttgtcggt acatcaagcc tgatttctgc atttagtcct atagtcaaat
    11701 cttatgaatg tgttgcttac gaagcaatat atctttcaac tgagataggc tattctggct
    11761 ccatttcatc ttttggcttc aatcgtgttg atggtactga tacattaccg tttgatagtg
    11821 tctcaatttt tatgaaacag acagcattta cttcgttaac cgcaggaaat gttgatacaa
    11881 ccggatatac tcttgtttat gagggtccat ttcctaataa tggcggtata ggatggcgag
    11941 aggttgcatt agatacatca tttacttaca gcacctcatc caatttaagc gtaatggtgt
    12001 tcaaacatac acaaactgct actctcagta caccggtgtc gcctcgttgg tattacacat
    12061 cagtttcttc taatagatca agaaggtact atggagctat accaatttct tctgttacta
    12121 gtcttagtgc aacaaatgtt ttaagtgata taagaataac cgttggaaca gttggtattg
    12181 ttgatatttc agcaggatta ttttctgttt atccaaaccc aacaaatgga atcattcata
    12241 ttgttctgca taattccgag atatcatctt tgaaaatatc agacattaat ggaagaattg
    12301 tcaaggaaat gtataatttg aaagatggaa gtttagattt gactaatctc aaggatggtg
    12361 tatataactt gcaatttttt gataaatcaa aaaataatat tgccaccaga ataattgtag
    12421 ttaaaaggta atagtcaatt gtgttattca gcaattatct ctgataaaat tactttacta
    12481 taagtttctg aatatttatt gatgaattag tttctgattt tacaaaatac actcccggtt
    12541 taaattgact gacatcaaaa aacgcaaagt tttgtccatt agactttgtt gtattttgtg
    12601 attcataaac catcttgcct gttacatcac tgattttaat ctgaagcaat gtgccaatgc
    12661 gactatagta ggggaggcat gtcactgccg atgcaggatt tgggaaaatt tgtcccaccg
    12721 ataactgatt taaaacttca gttattccag tagtctgtaa tacatcaaaa taaaaataac
    12781 cttgcggtgc aggcattggt cgtgtctgat gttttgaggt atttgaatat gcttcaatat
    12841 agtagtaaat tcttgttcca ccagattgtg caggaatgta cgtttcaaag taatcactca
    12901 tccctgatat agcattcatc gaaacagtat tataaggttg caaagtatct gtcctgtaat
    12961 ataaggttgc cgattgaatt ccactacggt gtttaataaa tgcttcaaca tgataggggt
    13021 taattgtatc gtaagtgttg ctaagttgtt gatgtgaaat cagaagcgga tcattagtgc
    13081 ctaaatcttt agtgatacaa tgcaatgctc ctatagcacc aatactttga agtgagttaa
    13141 taccaataac attatatccc ggtaatgcat ctctatatat ccgtaatgct gtgctgtctt
    13201 gaggaatatt ataagtagga acaataatag tattattgat gaacgatgaa ttggtgtaag
    13261 taaaataatc accgtttgta tttggataag caccattgtc agcaggcatt ggaatgcgga
    13321 ttattttata aggtgtgcca aatacggaat taaaattatt gataacataa agcagattgg
    13381 cttctatctg aggaccatct gcaacacctt gaggatattg tcccatcaag attgtttctt
    13441 cattaagaag cttcatgtgc atatcaatat gatggatagc atcataaggt aatgtaggca
    13501 ttttaatata tctgttaatg cccataaatt gtttcatgat agtatctatc tgacttatgg
    13561 ttaacgaggg attttcattc actataagat tgcttgagaa tcccgttcca aatccatcgc
    13621 acataaaatt accacctgta tgaaccaaac tgtatggcga cattgtggtt tggtataatg
    13681 gcgtatttag taatccggca attgccaccg gaacattatc atcgtcagga cgaggacgat
    13741 tatatatcca gtcaatagta actaaagtgt caacatcgtt tgtgtaagca ctccatggac
    13801 cgtaatcgcg tgaccatact gaattgaaag gggtattcac aaaacttaca tttatcgtat
    13861 ctacatttcc actgtttaaa tagttaatta cactttgctc attattggtt acaacataaa
    13921 cccggcattc tgttttagca gcacgtacaa tttcacgcaa aatagaaggg taacttgtcc
    13981 agccaattaa aattccttgc agctcttccc actcagcaat agttcgaacg ggtgatgatg
    14041 gtggagctgc aatctgatta ttttttaatt gattagacaa ttgttggaga tatgatggca
    14101 tctgcaacat ttctttttga gtcatatagt ttggaagaat ttcctgagca gaaacatttc
    14161 ctgtgaaaaa gagatatgct aaaataaatt taagtgcatt ttgcatgttg aggttattat
    14221 gaagcatcaa atataatcac tttcagattt atgccgtcat taaatagtat ttccaaaata
    14281 attgctttat ttgctctgta aaacagatat tatgaaatgg atgggcaggc gcgaaagcga
    14341 caatgttgaa gaccgcagga gattttcagg aggtaaaatt gccttaggtg gtggattagt
    14401 tggcataatt gtcattgtca tcaacttgct gatgggtggt gatcctaatc aacttctaca
    14461 acaaatgcag cagcaggcaa caactcagac aaccaccacg gaatcatctg ctgccgatga
    14521 tgaaatggca agttttataa aagtagcatt agcagataac gaagatatct ggaagaaaat
    14581 ttttcaggaa aacgaattag aatatcgtga gcctaaattg gttcttttta gagatgcaac
    14641 acagtccggt tgtggcaatg caagctctgc tgttgggccg ttctactgtc cggccgatga
    14701 gaaagtatat attgaccttt cattttttga tgagttgaaa agccgttttg gagcagaagg
    14761 cggtgatttt gctgttgctt atgtattggc acatgaagta ggtcatcatg ttcaacatct
    14821 tttaggcaca tcggataagg tacatcagat gcaggaaggt ttatcggaaa cagaagcaaa
    14881 taaattgtca gttgctttag aacttcaggc agatttttat gcaggagttt gggctaatca
    14941 aaatcaaaaa ttaaacaatg tgttggaaga tggtgatatt gaagaagcat taagtgcagc
    15001 aagtgcagtt ggcgatgata agattcaaaa aaaagcacaa ggctatgttg tgcctgatgc
    15061 ttttactcat ggcacttctg aacagagaat gtactggttt aaaaaaggct atcagacagg
    15121 aaaattacaa gaaggtaata cttttaaaga actgaacatt taatttattc aatctcaaat
    15181 gcaaactttt taaacccgat aaaaactaaa tgttgtctgt cgggttttta tgtttaggta
    15241 taaaattcaa aatcaaattt aaattatcat gcaaaaattc acatcatcgt taatggcact
    15301 catcattgcc acaacaacaa cttcaatggc gcaattaaaa acgccacaac caagtccaac
    15361 tcaaagtctg acacaggcat ttggtcttgg cgaaatcaaa atcgaataca gcagaccatc
    15421 agtaaaagga agaacaattt ttggcgacct tgttccttat ggtaaaatat ggcgcacagg
    15481 agccaatcaa tctaccaaag taacttttgg agcagatacc aaggtaaatg gtaatgatat
    15541 tgcggccggc acttatgcac tctacacaat tccaaacaca acagattggg aaattatttt
    15601 ttacaaagat ttaactcttg gcggaaatgt aggcgactat aaacaaacag atgaaatgat
    15661 gcgatttaag gtgaagccgg ttgctactgc cgataagatg gaaactttca ctatcaatat
    15721 caataacatt gcaccaactt catgcagtat tgatttgtta tgggataaaa caaaagtgag
    15781 tattcctgtt actaccaata ttgacgagaa tgttatgaag caaatagata aggctatggg
//
LOCUS       DAOOOL010000053        15463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_316_length_15463_cov_116.492926, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000053 DAOOOL010000000
VERSION     DAOOOL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15463
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_15463_cov_116.492926"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKU82_05960"
     CDS             complement(<1..706)
                     /locus_tag="PKU82_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ82454.1"
                     /translation="MNKRLQVIKYIIADFLSALMAWTMFFIYRKTYIEPGDSHFSKEL
                     LADPKFFTGILILPVCWVLFYAFLGTYNNIYRKSRLRDLAQTALASLIGVTIIFFALL
                     LDDTIVTYKSYYNTFITLLIIHYSITVFFRLIITTITGNKIKKRKIGFNTLLVGSSQN
                     ALHIFEELESQKISQGNKFVGFIHVDEKNGEYLGGKLPHLGGIAEIPEAINKHQVEEV
                     IIAIESSEHENIGRIIN"
     gene            complement(735..1595)
                     /locus_tag="PKU82_05965"
     CDS             complement(735..1595)
                     /locus_tag="PKU82_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82455.1"
                     /translation="MMRIKLLITVISIASIIQACNIINPAEPTPSYIQIDTIKLTTDI
                     TTQGSASHKIVDAWVYVDNQAVGAYEMPCKIPVLASGQHTIYIQGGILLNGINATHLQ
                     YPYYKFVDKTVTLKPGEVVNIDNLTTSYIDNLTFPLMEDFSGLFSFEKSGNSPVGMLG
                     EFDASQAMEGGYGVIRLDSGMYNFEVKSKEMILPNNGTNVFLEMNYKNSAPFLVILQA
                     TDPFGNVINNAIITLNAKTDWNKIYIELKPTMQITSSSKRFRILFGMQRTDASTKESF
                     YFDNVKVVHN"
     gene            complement(1592..2578)
                     /locus_tag="PKU82_05970"
     CDS             complement(1592..2578)
                     /locus_tag="PKU82_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ82456.1"
                     /translation="MLSIGVIGAGHLGKIHLKLLKELKSYQLKGFYDNDATTRQQVSE
                     TLGITAYADAESLIAACDVVDIVTPTLSHFKYAMIALNAGKHIFIEKPITNTLQEAEE
                     MISLAEQKGLKIQIGHVERFNPAFVAAQSKIRQPMFIEAHRLAEFNPRGTDVPVVLDL
                     MIHDIDVILSIVKSEVKLINTSGVSIISDTPDIANARIEFENGCVANLTASRISMKNM
                     RKARIFQGDSYIAVDFLKKKTEIISMRNTTENESPFAIIIDPGNGKPNKTIEFNNPVV
                     EESNAIKEELRTFAIAVENNTTPIVSATDGYKALKVAHQIMDKLSQQKFTES"
     gene            complement(2639..3112)
                     /locus_tag="PKU82_05975"
     CDS             complement(2639..3112)
                     /locus_tag="PKU82_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ82457.1"
                     /translation="MNVLSEFIQQESGQKIIIAHPESEEELNQYYQLRHEILRAPWNQ
                     PPGSEKVNDDNEAIHLSLLIDDLMIGVCRLHFNNADEAQIRFMGVKSEFRNQKLGDYL
                     MLAAENISKSNHCKSIFLQARVNAVPFYERNGYHIEAKTFLLFDTIQHYAMRKNI"
     gene            complement(3117..5927)
                     /locus_tag="PKU82_05980"
     CDS             complement(3117..5927)
                     /locus_tag="PKU82_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82458.1"
                     /translation="MPIEPYNTVLTLKSFFRLFIATLFAGLLSADHVMALYYQSVASG
                     NWNATTTWQSSVAAGGPWAPAGATPTSADQTVTIMAGTVVRITTGITIDEVVVSPGAT
                     LIANSSANLIINNGAAVDLIINGTFVDSLGGAANINFNAGATWQISAGATIVKCTVSS
                     CNNWQSSYQGGIASIPATANWIFRKVGLSNPSVSTTNGGTQAYYPNFIYENYTSTPFI
                     TGSYANSHFNGSASYTIIKGSMDIGGSGTNYVNFTSNNINPTIGVQVRGDVIIRPGNT
                     YNNIGTGTEVWGDVYMDGTWKYYNGNGRRLVFSGNANSTVWGSGHFQIHELTMAKILP
                     ASITLNRSLMVDSLVKFNSGKIYSSATNPLIFNVGAKANTVSNNNSFVEGPVRKLGST
                     AFVFPVGMANDVQPAGLSASGAPSDSGLFWTENFNNGCTSLCDANGYSGFNGAWSVMN
                     SGTNGNRANMFYVSCSEANRPVNTCAGGCATGGDATLHVGTSPCATCLICPTGDCGAI
                     YDTLASGGFDPTTDKRAVSPPINTVGKSNMSISFKYINVGDLRSPASSTDKALLEYSV
                     DNGLTWTALINIPRSSTVGGCALYKRWTAFGPLNLPAACNDLPNLLIGFRWINNNNSI
                     GATINTNGSFAVDEVNISGKSAESFTTEYFHINPTVIYNNVVNPPLDHVSRMEYWYMT
                     KEIGDKRKITLYWDGNSGGVTSLPDLRVARFNGASWDDLGNTGTTGTAAAGTITSDST
                     KDYGPVTLASVIALPGNPLPVELLSFNGNRHNDEIKLNWLTSSELNNHYFTLKRSGNN
                     LQYTELGKVNGAGTTNLPQRYDYIDSKPLRGINYYKLFQTDFDGTTEEKATLAIRFDA
                     YDGDVLVLPDFNHEQVLLQFSENNKEPVNIMLMDAAGRLMLQTVCSESNTCRLLTSHL
                     SRGVYVLRMQTADGVIVKRFFY"
     gene            6199..6681
                     /locus_tag="PKU82_05985"
     CDS             6199..6681
                     /locus_tag="PKU82_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOZ82459.1"
                     /translation="MAEAEFLKTEKEIFTEYMISNGLRKTPERYAVLEEAYRTNGHFN
                     VDMLFELIQKRKLRVSRATVYNTIEHLLKCGLITKHQFGETKFIYEKSFAYKQHDHLI
                     CGDCEKVFEFCDPRLYQIQSTVEKYLDFKITQHALNFYGQCNKLKQTGACEHFKVKKQ
                     "
     gene            6678..7019
                     /locus_tag="PKU82_05990"
     CDS             6678..7019
                     /locus_tag="PKU82_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOZ82460.1"
                     /translation="MNFSFTTSEKGNFCIIKLSGNLMEKGQANELMDEINTCIIRDMK
                     TFVLDLADLRFMSSSGLTVLLNILTKSRKAGGDTIICNINSQLKSVFTITRLTEVFTI
                     TENQEAAIAAI"
     gene            7034..8302
                     /locus_tag="PKU82_05995"
     CDS             7034..8302
                     /locus_tag="PKU82_05995"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOZ82461.1"
                     /translation="MKIDVLLGLQWGDEGKGKLIDVLAPDYDVIARFQGGPNAGHTLE
                     FDGIKHVLHTIPSGIFRENKLNIIGNGVVIDPLIFKKENDSLLKLGIDIKKNLWVSRR
                     AHLILPTHRLLDAASETMKGKTKIGSTLKGIGPTYMDKTGRNGLRVGDIERHDFKDAL
                     NYLLNKHAELLSFYNHPTVSKEDLEMWLESISTLREMTLVDSEYEINSLINSGQRILA
                     EGAQGSLLDIDFGSYPFVTSSNTITAGACIGLGVPPSAIGKVFGVFKAYCTRVGSGPF
                     PSELNDATGDELRKKGNEFGSTTGRPRRCGWLDLPALKYTAMVNGVTSLMMMKSDVLS
                     GFENLEVCNSYKVNGQSSVRMPFNVVNDIPEPEYITFKGWQNDISKANSFESLPSELH
                     NYIQAIEKYIGIPLDVVSVGPDRTQTIIRK"
     gene            8304..9026
                     /locus_tag="PKU82_06000"
     CDS             8304..9026
                     /locus_tag="PKU82_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82462.1"
                     /translation="MKIFSLFLFSLLICSNIYGQQLSIENKPALNEESKSVDSWVVHV
                     DQDMNFCFSAYSEFMRKNFDYRINRRSKIVHSVDKKLFTEISNLRIDQRAIFTQESKG
                     TAIAFQFSPGYDIHFGNTMYKEEFDRARAMVVNYVHFHYKMFYDDQIKNLRSKIKGLE
                     KNISNNQSKIEKNTKTVNLNAEDISNNAVGADKLREKNEKLEKDNVTLTTEIDKFRSQ
                     ITQLETEIDNANQSLKVVEAFN"
     gene            9035..9979
                     /gene="trxB"
                     /locus_tag="PKU82_06005"
     CDS             9035..9979
                     /gene="trxB"
                     /locus_tag="PKU82_06005"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOZ82463.1"
                     /translation="MSEKSEHVKCLIIGSGPAGYTAAIYAARADLKPVMYQGLQPGGQ
                     LTTTTEVENFPGYPKGTMGPEMMEEFRQQAERFGTDIRWGYASSVDFKGPVHKVIIDE
                     EKEITADTVIIATGASAKWLGLESEQRLNGFGVSACAVCDGYFFRNQHVAIVGAGDTA
                     AEEATYLAKLCTKVYMIVRRDEMRASKAMQHRVMNTPNIEVLFNTETKEVVGEKTVSA
                     VKVVNNITGAERILDVTGFFVAIGHQPNTDIFKGWLDMDKTGYIITKPGSTKTNIEGV
                     FACGDAQDKIYRQAVTAAGTGCMSALDAERYLADKGIH"
     gene            10067..10708
                     /locus_tag="PKU82_06010"
     CDS             10067..10708
                     /locus_tag="PKU82_06010"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOZ82464.1"
                     /translation="MTDTFKHQGLRRQLITELKSKGINDAAVIAAMTKIPRHAFMDNA
                     FIESAYIDKAFPIGSGQTISQPYTVAFQTLLLGIQKGDKVLEIGTGSGYQTAILCELG
                     AKVFSIERHKLLADKAKSLLEKLGYIPKIHFGDGFKGLPAFAPFDKIIVTCGAPDIPQ
                     SLFEQLKTGGVMVVPVGEDVQVMKTVVKKPDQTMEVSDFNSFRFVPMLGNKVR"
     gene            10892..11437
                     /locus_tag="PKU82_06015"
     CDS             10892..11437
                     /locus_tag="PKU82_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ82465.1"
                     /translation="MTDRELIEGCIRGDRNCQKALFEKFAGKMKVVCMRYVNNHEDAE
                     DVLQEGFIKVFKYISKFGFEGSFEGWIRKIMVNTALTKITRYSYKEEVALVHSNMPVY
                     DYGDVIAEMSAKELKAFINALPDGYRLVFNMYVIEGFSHKEIAESLSISEATSRSQLL
                     KARLALQEKLKKNKIAYSHAG"
     gene            11427..12746
                     /locus_tag="PKU82_06020"
     CDS             11427..12746
                     /locus_tag="PKU82_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82466.1"
                     /translation="MQDEQFDDLIRQKLNSEEESVSAGLWSRVEADLPVAKQGVFNRS
                     LLWLLLLFLIPAGAGWYYFTSNQTKISSLEGNRSSKQKVISNIASEVRNTDDKTMQNN
                     SVQSSDHNTLNNSIESNSSQIKYSSNANSTISANNNTVSKTLEPTYAISSTTNFNNQV
                     ENSEWDLLNPIQASLPEAASSQALISDKPTLEIKKTKRGIRVFADIYYAPEFAQHTRD
                     LGSSSPAYLKLRRNTETNIYSHSQGIRAGVWLSTNFFFKTGIDMFDMNERLSYTFIPG
                     MGNLPVGMRIDSINGYYINPLTNALVADTIVGSVNSTGEVIISNHYRFVNVPLMFGWR
                     FNKGLWSIQSSTGIYMNIYSGYKGKIIHESMQSLIDISSTSTPYKQNIGIGFSAEVIA
                     TYSIFKNIDVLIGPKYNYHLSDISRKGAPIQEKLSSIGMFSGIRYNF"
     gene            complement(12817..13401)
                     /locus_tag="PKU82_06025"
     CDS             complement(12817..13401)
                     /locus_tag="PKU82_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ82467.1"
                     /translation="MKKLFLSAIAVTMAFSLNAQTQWSADKSHSKIGFTVTHMLIAEV
                     EGNFKDFTASMTSTKDNFEDSKVEFKAPVSSINTNDDKRDEHLRGSDFFDMANHPEIS
                     FTSVTFKPAGDKKFKVVGNLTMRGVTRSVTLEATHIGTVEDPWGNTKAGFKLTGKVNR
                     KDYGIAWNKTLTSGGAVVSDEVAMNCMIELIKQK"
     gene            complement(13729..14301)
                     /locus_tag="PKU82_06030"
     CDS             complement(13729..14301)
                     /locus_tag="PKU82_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82468.1"
                     /translation="MFIDFGAGQVRLLGNRRYEIIYNMQLHVVDDIVSETSKMEARAG
                     AGVATPEYAVDQALARLDLIDHILYHLTGFTTTGMRGMQCTGVELSSVESSPTHDILT
                     WAVRLQTDTAKKVMIEVEPEVVVIPNSHFPPNPTYSQSQTQLRLQSIPFGEFALLLNC
                     LAQVYFSVWLIFRVYVFLQTFLVVSLIPDR"
     gene            complement(14600..14728)
                     /locus_tag="PKU82_06035"
     CDS             complement(14600..14728)
                     /locus_tag="PKU82_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82469.1"
                     /translation="MRKTLADDASRMALNEFLENFQRQGSVNANGLFIPWKATGGG"
     gene            complement(14891..15154)
                     /locus_tag="PKU82_06040"
     CDS             complement(14891..15154)
                     /locus_tag="PKU82_06040"
                     /inference="COORDINATES: protein motif:HMM:NF037113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HOZ82470.1"
                     /translation="MRDKIFINVGDVIKNPDEVWMTYYKNKSAQYRYVKFYGDKKTMI
                     VPVDVNMGMDENNKDNLLKIKTWFLLDKEEEKNKRVGVLIYKK"
BASE COUNT         4921 a         2721 c         2947 g         4874 t
ORIGIN      
        1 cattaataat tctgccaata ttttcatgct ctgacgattc aatagcaatg atgacttctt
       61 ctacctgatg tttattgatg gcttcaggaa tttcagcaat gccaccaagg tgaggaagtt
      121 ttcctcccag atattcgcca tttttttcat caacatgaat gaaacctaca aatttatttc
      181 cctgactgat tttttgtgat tctaactctt caaaaatgtg tagtgcattc tgactgcttc
      241 caaccaacag tgtgttgaaa ccaatttttc tttttttgat tttgtttcct gtaatagttg
      301 tgattattaa ccggaaaaaa actgtaatgc tataatgaat aatgagtaag gtaataaaag
      361 tgttgtagta gcttttgtag gtaacaatgg tgtcatctaa cagtagtgca aagaatatga
      421 tagtaacacc gattaatgac gcaagtgctg tttgtgccaa atcgcgcaat cgagatttgc
      481 gataaatgtt attgtaggtt cccagaaatg cataaaataa aacccaacat accggaagta
      541 ttaaaatgcc ggtaaaaaat ttaggatcgg caagaagttc tttgctaaaa tgagagtcac
      601 cgggttcaat ataagtttta cgataaatga aaaacatggt ccaggccatc aatgcagaca
      661 gaaaatcggc aataatatat ttgatgactt gtaagcgctt attcattact gataaaactc
      721 tgtgtaacta tactttaatt atgaactacc ttaacattgt cgaagtaaaa actctcttta
      781 gtacttgcat cagtacgttg catgccaaaa agaattctga aacgtttaga ggagctagtt
      841 atctgcatag ttggttttag ttcaatgtaa attttattcc aatcggtttt tgcatttaat
      901 gtaataatgg cattgttgat gacattgcca aatggatcag tagcttgcag aattacaaga
      961 aaaggagcag agtttttata attcatctct aaaaaaacat tggtgccgtt gttcggcaga
     1021 atcatttctt tagattttac ttcaaaatta tacatgcctg aatccaatcg tataacacca
     1081 tatccacctt ccatggcttg tgatgcatca aactcgccaa gcatgccaac aggactgtta
     1141 ccacttttct cgaatgaaaa taaccctgaa aaatcttcca ttaaaggaaa tgtaaggtta
     1201 tcaatataac ttgtagtaag gttatctatg ttgacaactt ctcctggctt taaggttact
     1261 gtcttgtcaa caaatttgta gtagggatat tgcaggtgtg tggcattaat gccgttcagt
     1321 aaaattccac cttgtatata gatagtatgt tggcctgatg ctaataccgg aatcttacaa
     1381 ggcatttcat aagcgcctac agcctgattg tccacatata cccaggcatc aactatttta
     1441 tgtgatgcac ttccttgcgt agttatatct gttgtgagtt tgattgtatc aatttgaata
     1501 taggaaggtg taggctcggc aggattaatg atattgcaag cttgaattat tgatgcaatt
     1561 gaaattacag tgattaaaag tttaatcctc atcatgactc agtgaatttt tgctgtgaca
     1621 atttatccat aatctgatgg gctaccttca atgctttata accatctgtt gcactaacaa
     1681 taggagtggt gttattttct actgcaatag caaaggttct taactcttct ttaatggcat
     1741 tactttcttc tacaactgga ttgttaaatt caattgtttt atttggctta ccatttcccg
     1801 ggtcaatgat gatggcaaaa ggactttcat tttctgttgt attgcgcatg gagattattt
     1861 ctgttttctt tttaagaaaa tcaacagcta tataagaatc gccctgaaag attcttgctt
     1921 tacgcatatt tttcatggaa atgcgactgg cagtaaggtt tgcaacacat ccattttcaa
     1981 attctattct ggcatttgca atatcaggtg tatcgctgat gattgaaacg ccactggtgt
     2041 taatcagttt tacttcagac ttcacgatac ttaatattac atcaatgtca tgaatcatca
     2101 ggtcaagcac aacaggcaca tcagtgccac gtgggttgaa ttctgccaaa cgatgtgctt
     2161 caataaacat aggctgacga attttacttt gtgcagcaac gaatgcagga ttaaatcgct
     2221 ccacatgtcc aatctgaatt tttaatcctt tttgttctgc caaggaaatc atttcttcag
     2281 cttcctgaag tgtattggta ataggttttt caataaaaat atgcttacct gcatttaaag
     2341 ctatcatagc atatttgaaa tgtgataatg ttggagttac aatgtccaca acatcacaag
     2401 ctgcaatcag gctctctgca tcagcataag ctgttattcc taaagtttcg gaaacctgtt
     2461 ggcgtgtggt agcatcatta tcataaaagc ctttgagttg atatgatttt aactctttta
     2521 aaagtttaag atgtatttta ccaagatggc ctgcgcctat aactcctatt gaaagcatga
     2581 aaaaatttgc gcgaaaataa gggtaaaatg gctaccttaa gtgaataatc acgagcaatt
     2641 aaatgttttt tctcattgca taatgctgaa tggtatcgaa taataagaat gttttagctt
     2701 caatatgata gccgttacgt tcgtaaaagg gaactgcatt tactcgtgct tgcagaaaaa
     2761 ttgatttgca atggtttgat ttagagatat tttcggctgc aagcattagg taatcgccta
     2821 atttttgatt tctgaattca gattttacac ccataaatcg aatctgtgct tcatcagcat
     2881 tgttaaaatg taatcggcaa acacctatca ttaaatcatc aatcagcaga ctaagatgaa
     2941 tcgcttcatt atcatcatta actttttcac tgcctggagg ttggttccat ggtgctctta
     3001 aaatttcatg tctgagctga taatactgat tcagttcttc ttcgctttca ggatgagcta
     3061 tgattatttt ttgtccactt tcttgttgaa taaattcaga aagaacattc acaactttag
     3121 tagaaaaatc tttttactat aacaccatct gctgtttgca tgcgcaacac ataaacacct
     3181 cttgaaaggt gagatgttaa taaacggcaa gtgttgctct ctgaacacac agtttgtaac
     3241 ataagtcgcc cggctgcatc cattagcatt atgtttacag gttctttatt gttttcggaa
     3301 aactgaagca atacctgttc atgattaaaa tccggcagaa ctaaaacatc gccatcataa
     3361 gcatcaaaac gaattgccaa tgtggccttt tcctctgtgg tgccgtcaaa atcggtttga
     3421 aaaagtttgt aataattgat tccgcgcaat ggcttgctgt ctatataatc gtaacgctga
     3481 ggtaaattgg ttgttccagc accatttact ttgccaagct ctgtgtattg taaattattt
     3541 cccgatcttt tcaaggtgaa ataatgatta tttaattcag aagatgtcag ccagttgagt
     3601 ttaatttcat cattatggcg attaccatta aaagatagta gttcaacagg tagaggattg
     3661 ccgggtaatg ctattacaga agctaaagtt actggtccat aatctttagt tgaatcggaa
     3721 gtaattgtac cggcagcagc agttcctgtg gtgcctgtat ttcctaaatc atcccatgat
     3781 gcaccattaa aacgtgccac tctaaggtcg ggcaggctgg tgacaccacc actgttacca
     3841 tcccagtaaa gtgtaatttt tctcttatca ccaatctctt tagtcatata ccaatattcc
     3901 atccggctta cgtggtcgag tggtggattt acaacattgt tatagattac ggttgggttt
     3961 atgtggaaat attctgtggt aaacgactct gcgcttttgc ctgatatatt cacttcatca
     4021 acagcaaatg aaccgtttgt gttaatagtt gcacctatgc tattgttatt atttatccat
     4081 cgaaagccta taagtaagtt tggtaagtca ttacaagcag cgggtagatt aagcgggcca
     4141 aaggcagtcc agcgtttata taaagcacat ccacctacag tacttgatcg tggaatgttt
     4201 attaatgctg tccatgtcaa tccattatct acgctatatt caagtaaagc cttatctgta
     4261 cttgaagcag ggcttcttaa atcgccaaca ttaatgtatt taaaactaat agacatattt
     4321 gattttccca cagtattaat tggtggcgaa acagctcttt tgtcagttgt agggtcgaag
     4381 ccaccacttg ctaaagtatc ataaatggca ccacaatcac cagtcggaca aataaggcat
     4441 gttgcacaag gcgaagttcc aacatgcaaa gtagcatcgc caccggtagc acaaccacct
     4501 gcacatgtat taacgggtct attggcttcg gaacaactaa catagaacat atttgctctg
     4561 ttgccattag ttccggaatt cataacagac catgcgccat taaaaccact gtagccattg
     4621 gcatcacata gcgatgtaca tccattgtta aaattttctg tccaaaacaa acccgaatct
     4681 gatggtgctc ctgaggcaga tagccctgca ggctgcacat catttgccat tccaaccgga
     4741 aaaacaaaag cagtactgcc taattttctt acaggacctt ctacaaaact gttgttgtta
     4801 gatacagtat ttgcttttgc tcctacgtta aaaattaatg ggttggtagc acttgaataa
     4861 atttttcctg aattaaactt taccaatgaa tctaccatca aagaacggtt taatgtaatg
     4921 cttgcaggaa gaattttagc cattgtcaat tcgtgaatct gaaaatggcc gctcccccaa
     4981 actgtgctgt tggcatttcc tgaaaaaacc aatctgcgcc cattaccatt ataatatttc
     5041 catgtgccat ccatataaac atcaccccaa acttcagtac ctgtaccaat attgttgtat
     5101 gtatttccag gacggattat aacatcacct cttacctgta caccaatggt tgggttgatg
     5161 ttattacttg taaagttgac atagtttgtt cctgaaccac ctatatccat actaccttta
     5221 atgatagtat agcttgcaga accattaaaa tgcgaattgg cataagaccc ggttataaat
     5281 ggcgtggaag tataattttc ataaataaaa ttgggataat atgcctgagt tccgccattt
     5341 gttgtactga cagaaggatt agacaaacct acttttctga aaatccagtt ggctgttgca
     5401 ggaatagaag caatgccgcc ttggtaactt gactgccagt tattgcatga cgatacagta
     5461 catttaacaa ttgttgcacc ggcacttatt tgccaggtag caccggcatt aaaattaata
     5521 tttgcagcac ctcctaatga gtcaacaaaa gttccgttaa tgattaggtc aacagctgcg
     5581 ccattgttaa tgattaaatt agcagaagaa tttgcaatta atgttgcacc cgggctgaca
     5641 actacttcat caatcgtaat acctgtagta atgcgtacta cagtaccggc cataattgtt
     5701 acagtctgat ctgctgatgt gggggttgcg cctgccggag cccaaggacc acctgctgct
     5761 acactacttt gccatgtagt tgtagcattc caattgcccg atgcaactga ttgatagtat
     5821 agagccataa catggtctgc tgataataaa cctgcaaaaa gtgtggcaat aaatagcctg
     5881 aaaaacgatt tcaatgtcaa aacagtgttg tatggttcaa ttggcaaaag tacttgatat
     5941 aattctaaaa cattgcattt ttgttgccgt tggcattaaa tattttttta cgacaaaaaa
     6001 tgaattttgt tattgttgtg atataggtct attgaggttt ttttcattta aaaatcagag
     6061 ataagcggaa agcaatttat caacaatttg gaagaaaaag taagtgaaga acaactattt
     6121 gtatctcagt tgtaattgga aattctataa cagtttgatt tattactttt gtagcaatta
     6181 aaatggcatt tatttaacgt ggcagaagcg gaatttttaa agacagagaa agaaattttt
     6241 acagagtata tgataagcaa tgggctacgc aaaacacctg aacgttatgc agtacttgaa
     6301 gaagcttatc gcactaatgg tcattttaat gtggatatgc tctttgaact gattcaaaaa
     6361 cgcaagttaa gagtaagccg tgccaccgtt tataatacta ttgaacattt attgaaatgt
     6421 ggcttaataa ccaaacatca gtttggcgag accaaattta tttacgaaaa gtcttttgcc
     6481 tataagcaac acgatcattt gatttgtggc gattgcgaaa aagtatttga attttgcgac
     6541 ccgaggcttt atcagattca gtcaacagta gaaaaatatt tagatttcaa aataacacaa
     6601 catgccctga atttttatgg acagtgtaat aaactcaaac aaacaggggc atgcgaacat
     6661 tttaaagtaa aaaaacaatg aattttagtt ttaccacatc agaaaaggga aatttctgta
     6721 ttattaaact ttcgggcaac ctcatggaga aaggacaggc taatgaactg atggacgaaa
     6781 tcaatacctg tattatcaga gatatgaaaa cgtttgtgct cgatttagca gatttgcgtt
     6841 ttatgagcag cagcggactt actgttttat taaacatcct gaccaaatcg cgcaaggccg
     6901 gtggcgacac cattatttgc aatataaata gtcagttaaa atctgtattt actattacac
     6961 gattgacaga ggtttttacc ataacagaga atcaggaagc tgctattgct gccatctgaa
     7021 taaaaaaata attatgaaaa tagacgtatt acttggactt cagtggggcg atgagggcaa
     7081 aggaaaactg atagatgttt tagctcccga ctatgatgtg attgcacgtt ttcagggtgg
     7141 gccaaatgca ggacatacct tggagtttga tggaataaaa catgtgttgc ataccattcc
     7201 gtcaggaatt tttcgtgaaa ataaacttaa catcattggt aatggtgtgg taatagatcc
     7261 gctgattttc aaaaaagaaa atgattcact gcttaagtta ggaatagaca taaagaaaaa
     7321 tctttgggta tcgcgaagag cacatctcat tctgccaaca catcgtttgc ttgatgctgc
     7381 atcagaaacc atgaagggta aaacaaagat tggttctaca ctaaaaggaa taggtccaac
     7441 ctatatggat aaaaccggac gtaacggtct gcgcgttggc gacattgaaa ggcatgattt
     7501 taaagatgca ttaaattatt tattaaacaa acatgcagaa ctgctttcat tctacaacca
     7561 tccaacagtc agcaaggaag acctcgaaat gtggcttgag agtatttcaa cacttcgcga
     7621 aatgacattg gttgacagtg aatatgaaat aaacagtttg attaattccg gccagcgtat
     7681 tttggcagaa ggggcacaag gctcattgct tgatattgat tttggatctt acccatttgt
     7741 tacgtcaagc aatacaatca cagcaggtgc atgtatcggg cttggtgttc ctccttcggc
     7801 aataggtaaa gtgtttggtg tgttcaaagc atattgcaca cgtgtaggca gtggtccttt
     7861 cccatctgag ttaaatgatg caacaggaga tgagttgcgt aaaaaaggca atgaatttgg
     7921 tagtactacc ggtcgcccaa gacgatgcgg atggctcgat ttacctgctt tgaaatatac
     7981 tgcaatggtt aatggcgtta caagtctgat gatgatgaaa tctgatgtac ttagtggttt
     8041 tgaaaactta gaggtttgta attcttataa agttaatggg caatcttctg tacgaatgcc
     8101 gttcaacgta gtgaatgata ttccggagcc ggaatacatt acctttaaag gatggcagaa
     8161 tgatatcagt aaggcaaata gttttgaaag tttaccatct gaattgcaca actacattca
     8221 ggcaattgaa aaatacatag gtattccatt agatgttgta tcagttggcc ccgatagaac
     8281 gcaaacaata atcagaaaat aaaatgaaaa tattttcatt atttttattc agcttactta
     8341 tttgctccaa catttacgga cagcaactgt caatagaaaa taagcctgct ttaaacgaag
     8401 agtcaaagag tgttgattcg tgggtagtac atgtagatca ggatatgaat ttttgtttct
     8461 ctgcttacag tgagtttatg cgtaagaatt ttgattatcg tattaaccgc agaagtaaaa
     8521 ttgttcattc agtagataaa aaattattta ccgaaatttc aaatctgcgg atagatcagc
     8581 gagcaatatt tacacaagaa tcaaaaggta ctgcaatagc ttttcaattc agccccggct
     8641 atgatataca ttttggcaac accatgtata aggaggagtt tgaccgtgca agagccatgg
     8701 ttgtaaacta cgtgcatttt cattacaaaa tgttttatga tgatcagata aaaaacctga
     8761 gatcgaaaat caaaggatta gaaaaaaata tcagcaacaa tcagtcaaaa attgaaaaaa
     8821 atactaaaac tgttaatctt aatgcagaag atatttcaaa taatgctgta ggagcagata
     8881 agttgcgcga gaagaatgaa aagctagaaa aagataatgt gacactaaca acagaaatag
     8941 ataaattcag atcacagata acgcagttag aaacagaaat tgacaatgct aaccaaagcc
     9001 ttaaagtagt tgaggcattt aattaaaata tgcaatgagt gaaaaaagtg aacatgtaaa
     9061 gtgcctgatt attggctcgg gtcctgcagg atatacagca gcaatttatg cagctagggc
     9121 agatttaaaa cctgtaatgt atcagggatt gcaacccgga ggtcagttga caacaacaac
     9181 agaagtagaa aatttcccgg gctaccctaa aggtacaatg gggccggaaa tgatggaaga
     9241 atttcgtcag caggcagagc gatttggcac tgacatcaga tggggatatg catcatctgt
     9301 tgattttaaa ggacctgtac ataaagtaat cattgacgaa gaaaaggaaa ttacagccga
     9361 tacagtaatt attgcaacag gtgcatcagc taagtggctt ggattagaga gcgaacaacg
     9421 actgaatggt tttggtgttt ctgcatgtgc cgtttgcgat ggatattttt tccgcaatca
     9481 gcatgtagct attgtagggg caggtgatac tgctgccgaa gaagcaacat atctggcaaa
     9541 gttgtgtaca aaagtttata tgattgtgcg cagagatgaa atgcgtgcat caaaagcaat
     9601 gcaacacaga gtaatgaaca caccaaatat tgaagtcttg tttaatacag aaactaagga
     9661 ggtggtagga gagaaaacag tgtctgctgt taaagttgtt aataatataa caggtgcaga
     9721 acgcatactt gatgtaacag gcttttttgt ggcaattggg catcaaccaa ataccgatat
     9781 ttttaaaggg tggcttgata tggataaaac cggttatatc ataaccaagc cgggctcaac
     9841 caaaacaaac atagaaggtg tttttgcttg tggcgatgca caggataaaa tttacagaca
     9901 ggctgttact gctgcaggaa caggttgtat gtctgcttta gatgcagagc ggtatttggc
     9961 agataagggc attcattaaa aaaatcatta gcattttttt gttaaaaatt ttattgattc
    10021 agcaataaac attgttgagt gaatggtaaa tttgtttgca atgaatatta cagacacttt
    10081 taagcatcag ggattaagac gacagctaat tacagaatta aagtctaagg ggataaatga
    10141 tgcagccgta atcgctgcca tgacaaaaat tccacgtcat gcatttatgg ataatgcatt
    10201 tatcgaatct gcctatattg acaaagcttt tcccattggt tcaggtcaaa caatttcaca
    10261 accctatacg gttgcttttc aaaccttgtt gcttggtatt caaaaaggtg ataaagtgtt
    10321 ggaaattgga acaggctccg gctatcagac ggctatactt tgtgagttag gtgcaaaagt
    10381 attttctatt gagcggcata aattacttgc cgataaagca aaaagtttac tcgaaaaatt
    10441 gggttatatc cctaaaatac attttggcga tggctttaaa ggactgcctg catttgcacc
    10501 atttgacaag ataattgtta cctgtggcgc acccgatatt ccacagtcat tgtttgagca
    10561 attgaaaacc ggaggagtga tggttgttcc tgtaggtgaa gatgtgcagg taatgaaaac
    10621 ggttgttaaa aaacctgatc aaaccatgga ggtttcagat ttcaactcat tccgctttgt
    10681 tcccatgctt ggtaataaag taagataagt acattatagt tttattaaaa aacattcaca
    10741 ttttttgagt aatatttttc tctcacttag atttatggca agtgaaggtg gtgttttgct
    10801 atgttttaat taattttacc aaagcgcaaa agtcaggcaa cgtttcccgg acttattgag
    10861 tctttatata tgaaatgcat tcaatactgt tatgacagac cgtgaactaa ttgaaggttg
    10921 tattcgcggt gacagaaatt gccagaaagc tttattcgaa aagtttgccg gtaaaatgaa
    10981 agtcgtttgc atgcgctatg tcaataatca cgaagatgca gaagatgttt tacaagaggg
    11041 gtttataaaa gtgtttaagt atatatcgaa atttggtttt gaaggatctt ttgaaggttg
    11101 gataagaaaa ataatggtta atacagcgct tacaaaaatt acacgctaca gttataaaga
    11161 agaagttgcc ttggttcata gcaacatgcc tgtttatgat tatggtgatg tgattgcaga
    11221 aatgtctgcc aaagagctga aagcattcat aaatgcattg cctgatggtt ataggctggt
    11281 gtttaacatg tatgtgatag aaggtttttc gcataaagaa atagctgaga gcctgagtat
    11341 ttcagaagca acatcgcgta gtcagttatt gaaagcacga ttggcattac aagaaaaatt
    11401 aaaaaaaaat aaaatagcat acagtcatgc aggatgaaca atttgatgat ctgataaggc
    11461 agaaacttaa ctccgaagag gagtcagtaa gtgccggact gtggagtagg gtagaagcag
    11521 atttacctgt tgccaaacaa ggagttttta atagaagttt actatggttg ctacttttat
    11581 ttttaattcc agcaggtgca ggatggtatt attttacttc aaatcaaaca aaaatttctt
    11641 cattagaagg taatcgttca agcaaacaaa aggtgattag taacatagca tctgaggtaa
    11701 ggaatacaga cgacaaaacg atgcaaaaca attctgttca gtcatcagat cataatactt
    11761 taaacaattc aatagaaagt aattcgagtc aaattaaata tagctctaac gcaaatagca
    11821 ctatctcagc taataataat actgtctcaa aaactttaga accaacctat gcaatttcat
    11881 caacaacaaa tttcaataat caggttgaaa atagtgagtg ggatttgctt aatccaatac
    11941 aggcatcatt gccggaggca gcaagtagtc aggctcttat ttcagacaaa cctactttag
    12001 aaataaaaaa gacaaagaga ggaattcgtg tatttgcaga tatttattat gcacctgagt
    12061 ttgcccaaca tacacgcgac ttaggaagca gcagccctgc ctaccttaag ttgaggcgca
    12121 atacagaaac aaacatttat tctcacagtc agggaattag ggcaggtgtt tggttgagta
    12181 ccaatttctt ttttaagaca ggaattgaca tgtttgatat gaacgagcga ctgagctata
    12241 catttattcc gggcatggga aatctccctg taggaatgag aattgattct atcaatggtt
    12301 attacatcaa tccactcaca aacgctttag ttgctgatac gattgtaggt tcagtaaatt
    12361 ctacaggaga agtcatcatt tcaaatcatt atcgttttgt caatgtacct ttaatgtttg
    12421 gatggcgatt taacaaaggt ttatggagca ttcagagcag cacgggaatt tatatgaata
    12481 tttattcagg gtataaagga aaaatcatac atgaaagcat gcaatcgcta attgatattt
    12541 cttccacctc aacaccttat aaacaaaata ttggtatagg tttttctgca gaagtaattg
    12601 caacatacag tatctttaaa aacattgacg tgctcattgg tcctaaatat aattatcatt
    12661 taagcgacat cagtcgcaaa ggtgcaccca ttcaagaaaa gttgagtagc ataggtatgt
    12721 tttcaggtat cagatataat ttttgattta ggtttggatt aattgaataa aaaaggcagc
    12781 cttttcagag gctgcctgca aaaaattaat tcacatttat ttttgtttga tcagttctat
    12841 catacaattc atagctactt cgtcactcac cacagcacca cctgatgtta aagtcttgtt
    12901 ccaggctata ccataatctt tacggttaac ttttccggtt aatttaaagc cggcttttgt
    12961 gtttccccat ggatcttcaa cagtacctat atgcgttgct tctaaggtta cacttcttgt
    13021 tacaccacgc atggtaaggt taccaacaac tttgaatttt ttatcacctg caggtttaaa
    13081 agttacactg gtaaaagaaa tttctggatg atttgccata tcaaaaaagt ctgacccacg
    13141 tagatgctca tcacgtttat cgtcattggt attgatagac gaaacaggtg ctttaaactc
    13201 aactttgcta tcttcaaaat tatcttttgt agaggtcatt gaagccgtga agtctttgaa
    13261 atttccttct acttcagcaa taagcatgtg tgtaacggta aaaccgattt tagaatgact
    13321 tttgtctgcc gaccattgtg tttgtgcatt aaggctgaaa gccatagtta ctgcaattgc
    13381 acttagaaat aattttttca ttttttatat tttttgattt atggtgcaat gatagcccaa
    13441 cgataaacat taaatattaa cctagattaa cttcaaaaaa attaattaaa atttcagttt
    13501 tatatttgta acaacatata agatattatg cagttgcaac aatgtcataa aaatattact
    13561 attgaattat taatccgaaa gatgtgataa agttttttct aaaagcatgg ttagtgttag
    13621 ttttgatttg tattgtcatt ctcttttatg atgactataa aaaagaaaaa cagagagagt
    13681 atgaaaggtc attacctaaa caacctgaat gggatacagc aacatcacct acctatcagg
    13741 aattaatgag acaactaaga aagtttgaag aaagacataa acacgaaaaa ttaaccaaac
    13801 agaaaaatat acctgtgcaa gacagtttaa caaaagagca aattcgccaa agggaatgga
    13861 ttgaagacga agttgagtct gagattgaga ataggttgga ttaggaggaa agtgactatt
    13921 cggaataacc acaacttccg gctctacttc aatcattacc ttcttggcag tgtcagtctg
    13981 caagcgtaca gcccatgtta gtatatcatg tgtggggctg ctttccaccg aacttaattc
    14041 aacacctgtg cattgcatgc ctctcatgcc tgttgtcgta aatcctgtaa ggtgatataa
    14101 tatgtggtca atcaggtcga ggcgagccaa tgcctggtca actgcatatt caggtgtggc
    14161 aacaccggca ccggcacgcg cttccatctt gcttgtctca ctcacaatat catccaccac
    14221 atgcaattgc atattatata taatctcata tcgtctgttg ccgagcaatc gaacttgacc
    14281 tgcaccaaaa tcaatgaaca ctgccttacg tgggaatggt aatgagttgg cattttattt
    14341 gtatatttgt tattcaatta agcggttaat cttgcaaggg gtgaggggct ttaaatgtag
    14401 ttccatcccg cttcacaata gtgagattaa ccgtttattt ttttataaat aagtattccc
    14461 ttccttacat tgccctcatc tttatctaac ttgtaccacg actttatatt aagtcttccg
    14521 tccacttcaa ccggcaccgg catcattgta gaatttaacg tatgtgtatt ttgccgccaa
    14581 aaatggctga tgctttgcct tagccaccac cggttgcctt ccaaggtata aataagccat
    14641 tagcattaac agacccttgt ctttggaaat tttccaaaaa ttcatttagt gccattcgac
    14701 tggcatcatc ggccagtgtt ttgcgcattt cataatatcc tcgtaatata ttgcgccacg
    14761 gatttttatt tttttgttgc attggtatga tttttatttt atatttgtat ccgaaacagc
    14821 aaaatctgtt gctttggtct gatgttggcg taggtaacgc gagggatgat catcaacaga
    14881 tttagctgtt tcacttttta tatattaata ctcctaccct cttatttttt tcctcctctt
    14941 tatctaaaag aaaccatgtt tttattttca ataagttatc cttgttgttc tcgtccattc
    15001 ccatattaac atctaccgga acaatcattg tttttttatc gccataaaat ttaacatatc
    15061 ggtactgagc ggatttattt ttatagtagg tcatccaaac ctcgtctggg tttttgataa
    15121 catcacctac gtttataaat attttatcgc gcatttcctt atcacttaat tatttattgc
    15181 ctttcgcatg gggcgttatc atttgcttta ttcgcaagtg tttttattgt ttaaggcgct
    15241 catgaacctc gttcctcggt ttcaactttt aggatttaga actttcaaac cttcaaactt
    15301 tgtaacattc aaaactcagg tgttgtgatt tgctttattc gcatgtgttt ttattttttt
    15361 taaggcgctc atgaacctcg ttcctcggtt tcaactttta agaagtacaa ctttcaaacc
    15421 ttcaaacttt gtaacattca aaactcaggt gttgtgattt gct
//
LOCUS       DAOOOL010000054        15315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_322_length_15315_cov_77.753014, whole genome shotgun sequence.
ACCESSION   DAOOOL010000054 DAOOOL010000000
VERSION     DAOOOL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_322_length_15315_cov_77.753014"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKU82_06045"
     CDS             complement(<1..526)
                     /locus_tag="PKU82_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82471.1"
                     /translation="MQNSKGRKRGNSDKPQAGRYKSEGRPSRGKKFGSSTDKSKEEFV
                     KSGNSYSKNKFSGKRRFDADESGFERKRKNFGSDDNAGFEKRERPSRFKKEGGFEREK
                     RSFKSSDSPRPYRKRDEGFDGEERPSRFKKEGGFEREKRSFKSSDSPRPYRKRDEGFD
                     GEERPSRFKKEGGFE"
     gene            complement(534..1259)
                     /locus_tag="PKU82_06050"
     CDS             complement(534..1259)
                     /locus_tag="PKU82_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOZ82472.1"
                     /translation="MQTKLIALTAIIGMMLNGKMAHSQTPVNMSETRKIEVTGTAEMK
                     VIPDEIFVVITIQEYTENKKKTDINEVRKNFLAACTKAGIAKENISIQGMNGYNNSGW
                     YWQRKKKTPDFVASSSYIIKFNSGEQIERLMSQLDDKGTQNMYISSTSHSKMDEFKKQ
                     VKTEALQAAKAKATYLCEGIGEKVGKVLLIKEVEGTNYAEPMMMKSRMMEASMADSNF
                     EPSGLEFQKITIQMEILAQFAIQ"
     gene            1373..1882
                     /locus_tag="PKU82_06055"
     CDS             1373..1882
                     /locus_tag="PKU82_06055"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOZ82473.1"
                     /translation="MKAKTAKESLTIQTEIVLPNDTNVLGNLMGGRLLHWMDVCSAVA
                     AHRHCGRVVVTASVNNVSFNQPIRLGDIVTIQAKVSRSFTSSMEVFMEVWVERNATGD
                     KIKCNEAIYTFVAVDQQGSPIAVPELIAESEEEKKRFDGALRRKQLALILAGKMKPAD
                     ATELKALFF"
     gene            1988..2380
                     /locus_tag="PKU82_06060"
     CDS             1988..2380
                     /locus_tag="PKU82_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOZ82474.1"
                     /translation="MEKSIQTYNNSQSQEDKKICDLLADSIDTVLKTAECKIWHAHPV
                     WFIDENPIVGYSKQKLGWRLMFWSGIDFEEEGLNVHGKKFKDASVFYTSIEQINIKDL
                     KRWLKKSITIQWDYKNLIKRKGKLVRLK"
     gene            2386..2748
                     /locus_tag="PKU82_06065"
     CDS             2386..2748
                     /locus_tag="PKU82_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOZ82475.1"
                     /translation="MNSTEKNIERVAKMSFASIYPLYISKIQKKNRTSEELNQVITWL
                     TGYSVKELQKHLDNKTTFQEFFQQAKINPNASKITGTICGYKIEEIENPFMKKVRYLD
                     KLVDELAKGHPLTKILRS"
     gene            complement(2761..3153)
                     /locus_tag="PKU82_06070"
     CDS             complement(2761..3153)
                     /locus_tag="PKU82_06070"
                     /inference="COORDINATES: protein motif:HMM:NF022264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HOZ82476.1"
                     /translation="MKQRISVVMGLLIMVTMVSLSFKPDNWRILGTKTVDYQLDKDVL
                     DVQLRDGVFQKLKFVVRGGALNMHKVIVHFENGGEQEIDMRHSFDKNSQSRVIDLKGN
                     KRIIEKIVFWYDTKNASNRKAKVTVLGR"
     gene            3338..4216
                     /locus_tag="PKU82_06075"
     CDS             3338..4216
                     /locus_tag="PKU82_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOZ82477.1"
                     /translation="MEKRSVEIVVSPREPHFVGDGFRVHNFIPSGYRLDMQRMNPFIM
                     LDYNSKFHFDPSDTPKGVGVHPHRGFETVTIAYKGKVEHHDSSGGGGVIGEGDVQWMT
                     AASGVLHKEFHEKEWSKTGGEFQMVQLWVNLPAKDKMSKPKYQALENSKINRFKSDDK
                     KTEIEIIAGEYKGVKGSASTFTPLHLYNVKMAKDAKTEMNLPKNFNTALLVIEGSITI
                     NGTEKVDTNHFALMDNDGESFTIDSNENAVYLVLSGEPINEPIAAHGPFVMNTRQELV
                     QAFEDFNQGRFGTLQE"
     gene            complement(4241..4531)
                     /locus_tag="PKU82_06080"
     CDS             complement(4241..4531)
                     /locus_tag="PKU82_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOZ82478.1"
                     /translation="MNFRIKENALIAWIAARFLGYKTMAIVIGKTIYLHGASVKTFQS
                     DSKWMKHELKHIEQFKRYGFLRFIFLYIIESIRHGYINNRFEIECREAENQD"
     gene            complement(4544..9658)
                     /locus_tag="PKU82_06085"
     CDS             complement(4544..9658)
                     /locus_tag="PKU82_06085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82479.1"
                     /translation="MKKIFGFIVVFLTLSINNLLYGQQPSSCTNLDFELGNFVGWTGK
                     TGSCCPISMFSNGIVTGQHTIMSGTGFDPHSNNQIPVVAPGGTYSARLGNDQAGSQAE
                     QLSYSFQVTPQTELFIYRYAVVLEDPVHDPTEQPRFEIRVFDQAGNPVGCGTYNVVSG
                     AGIPGFQTWLDPNIGSDIHFKTWTTVGIELSSYMGQTVTIEFSTGDCSLGGHFGYAYV
                     DAYCSSMTVTSDYCPGSSTALLIAPPGFASYLWSNGATTQTITINNPVIGATFSVQMT
                     SVTGCVVTLNQVIAVTQLFANFNFNPGCESGTQFFDSSYVLSGSPVNQWLWDFGDGTT
                     STMQNPWHQFPASGNYNVTLTATNAGGCTKSITKSISILPSPVSDFNFTPACPGQAIS
                     FNQNASFPSGTITSYEWLFGDGLPSSAVPDPTHVYSGMGPYDVSLIVTGNNGCVDTIV
                     KPVAPMPDPVAGFTFSVQCMNNTVTFTDVSQLTGGTITAWQWDFGDGSPLDNTANPVH
                     VYNGGGNYAVQLIITGSNGCTDTITKGVAMASLPTAAFTNSGACESQFTAFNDASSLT
                     GGSISSWLWYFGDGTNSTLQNPFHQYNGTGSFNISLVVLGDNGCRDSMARSITVKPSP
                     VASFTSLPVCPGQQAQFNSTAVAPAGETISSWIWNFGDGSAQTAGQNVNHTFTGAGTY
                     AVMSIVGASNGCIDSVVNNISTSITPVAKFSAPSGCALVPLSFSSTSTISSGTITGWD
                     WDFGDGTAHGTTAATTHAYAAPGSYNTYLTVTSNNGCTHTVLHEVSVSGKPVPSFTNS
                     SSCVNDSINLNSVSTSANGSINYLKWDFGDGTFLTGTDSVFHVYADTGFVQIGLIVGN
                     TLGCSDTVYRQIYIKPLPVAGFRWDEMCQSAPGSFYDTSVLINGNISGWLWDFGSGTT
                     NTANPQFAFSNYGVQPVTLTVTGSNNCKATISRNITVRPKPLAQFVSSSACVNAAETF
                     QNTSLISSGNIVSWQWNFGDGHSSISQHGQNTFTTDGYYQVQLFAISDFACADTALDT
                     IRVFPLPVANFFAPHVCLGNATVFNDLSQPISDSLITWSWNFGDGSTSTMPTPSHIFA
                     QDTIYPVSLTVQNEGFCSKTITKNVIIRPLPEVGFTFANPCVGSPVQFQDTSALSGYN
                     INWWQWNFGDSATASIASPVHTYSLSGNIFVKLIVRATNGCSDSVSVPVLVNHLPDAV
                     FSVSDICEQAVLTPNNQSALSGGNITNTLWQWGDNTSDSIFSPTHFYQSDGNFNINLE
                     VTGSNGCKNDTTIAIVIHDKPIASFTHSNACQLAPVAFYDQSSTVNDSLQIWQWQFGD
                     GDQSNLQSPVHDFGAYGVYYTELIVTNSFGCRDTVADSVTVAPKPQAAFSSVNVCKDQ
                     PMIFNNSSSVVAGSIVSNQWSFGDGSSAAVANPTYTYLSTGDYEITLVVTTANQCVDS
                     IKHIYRVYQLPTAGFIADTVEGCQPLLVLFSDSSVANEGLITMWNWNFDNGASDTLTN
                     PAAIVYDQAGYFSPVLYIETNLGCRDTFLRQDYIHVFAKPEAAFIAEPLSNTILHPLI
                     EIKDQSWNTVSWFYDFGDTTNTTEQNPSHWYKQPEHYTIIQYVESADGCKDTALISIE
                     IKNDFTFYMPNSFTPNQDGFNETWNAFGIGITEFNVQVFNRWGQLLFTSNDLSKGWDG
                     TYSGNKVQQGVYVYKATVRDLFNKQHFYVGNVNVIR"
     gene            complement(9887..12634)
                     /locus_tag="PKU82_06090"
     CDS             complement(9887..12634)
                     /locus_tag="PKU82_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ82480.1"
                     /translation="MKSRKTNQFILIAAITALLSANVFAQDKAKSTTTPVKVTSVEGI
                     TEYKLSNGLRVLLFPDPSKSTITVNITYLVGSRMEGYGESGMAHLLEHMVFKGSTNHT
                     NIPQELTAHGARPNGTTWYDRTNYFETFNATEENLRWALSLESDRMVNSFINKKDLES
                     EFSVVRNEFERGENSPSSVLEERVMSTAYLWHNYGKSTIGSKEDIERVPIENLKAFYH
                     KYYQPDNAVLTIAGKIDEQKTLQLVSEYFSPIPKPERKLQETFTVEPAQDGERNVVLR
                     RVGDVQVASCGYHIPAGAHADYAAIEVLIEALTNEPSGRLYKALVETKKATSTSGYSF
                     QLKDPGYAYFYAEVLKDKPLDTVMNTMKGIFDNLSKNPITNEEAERGKNAILKQLTLM
                     FNNSDRVGLSLSEFIAQGDWRLYFIYRDALEKVTTADVNRVAAEYFKPSNRTTGLFIP
                     DATPDRAVIPASPDVNALVKDYKGKKALAEAEAFDPSPTNISNRIKQATISGGAKYTL
                     LKKTTRGGNVSVNITLRMGNAAVMQNKGTAASFTASMLRRGTKNKSFQQINDEIDKLK
                     SNVGINGNGQTVNVSVTTTKENLSGILNLIGEIIKQPAFAQAEFDKLKEEQISDLESS
                     RSEPQAIAFRVYSKTLNPYPKDDFRYEMSFDEEKEALNKLTLADVKNFYERFYTTSNA
                     TAAIVGDFDEPSLIISLNNLLDKWGSPVLYERAKEPYFDVTPVNEKINTPDKANALMV
                     AGMNLPLKDDNADYPALMVGNYMLGGGFLNSRLATRIRQKEGISYGVGSQLQASSLDE
                     SGGFLSYAIYNPDNSDKLIAAWKEEVQKMLNEGFTNAELTDAVKGLIQARSVSRSQDR
                     ELCSKLNSYTYLGRSIKWDEEFENKIKALKVEDVNAAMKKWINPNKVSFVQAGDFERK
                     K"
     gene            complement(12668..13519)
                     /locus_tag="PKU82_06095"
     CDS             complement(12668..13519)
                     /locus_tag="PKU82_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ82481.1"
                     /translation="MKAATLIYVILFSCLFAVSDSNAQGLCGTVIDTAKMQAEAAANL
                     NSSQNQRGLFFPVNCLNKTLSITAYIFRDSLAADGITQASILSALQILNNDFAPMCLS
                     FSICEFVYVNNYNYNDFYALKHENEVYNLYYTPNTINVYFSNTLYDNTNSSVCGYAHF
                     PGGRDMIFLNKSCVGDGKTFSHEMGHFFGLYHTFETANGVELINGTNCLVAGDLICDT
                     PADPNGLNGADCQMLPYLPDPSGNWYVPQIGNIMSYYSPGCKCGFTTQQFNWMIQQYL
                     TNRNYLW"
     gene            13660..>15315
                     /locus_tag="PKU82_06100"
     CDS             13660..>15315
                     /locus_tag="PKU82_06100"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82482.1"
                     /translation="MKRLFFFILFIGIISNVSAQITASTTSGCVPLVNVNFTSPAGLT
                     NIDWDFGDMTSSNLPNPVHTFFTAGSFTVVCTGLLSGNAFIDSIKINVFRKPFTSLTA
                     TPPLSGCVPLTVHFNDSSVGGGGTAIVSRQWAFGDGATNVGNNSSPTNIYSLAGSFTV
                     SLKVTDANGCDTSISVPNYINTSIQPNAVITTVPSPPIGCTPPFTVAFSASASTSNST
                     TGTGLTYFWNFGGGNTSTVVTPPSINYPTSGTYNISLTVTDNNNCPKTVTTQVVINPP
                     LASFFAVGAVNDTVCPTVTFRNQSIGTNPFYNYGDGTSGVDSIHTYAPGTYQVTLQVL
                     NGICTDDTTITIHVENVVANFTSTPSYSCSWPFTVQYTNITSGAGTYQWIFGDGTTSN
                     LANPSHTFYRPDTNQYTIYDYYYYTDQLTFTSVHGCSATVAKQKNDTVFSITARFMPD
                     KSIGCAPMTVTFSDSSRSRETIVNYTYLFGDGTQTTGSNGVVTHTYNTPGIYYVRLVV
                     TNSAGCKDTSFIIPIYVGSAVTTSFTVVPTTICAYTPVQFNVLN"
BASE COUNT         4661 a         3019 c         2762 g         4873 t
ORIGIN      
        1 gctcaaaacc accttctttc ttaaagcgcg atggtctttc ttcaccatca aaaccttcgt
       61 ctctttttct gtaagggcgt ggactatctg atgatttaaa acttcttttc tctcgctcaa
      121 aaccaccttc ttttttaaag cgcgatggtc tttcttcacc atcaaaacct tcgtctcttt
      181 ttctgtaagg gcgtggacta tctgatgatt taaaacttct tttctctcgc tcaaaaccac
      241 cttctttttt aaagcgcgat ggtctttctc ttttttcgaa accagcgtta tcatcacttc
      301 caaaattctt ccgttttctt tcaaagccgg actcatcagc atcaaacctg cgtttgcctg
      361 aaaatttatt ttttgaataa ctgtttcctg actttacaaa ctcctctttg gatttatcag
      421 tgctacttcc gaattttttt cctcttgaag gacgtccttc agatttgtat ctgccggctt
      481 gcggcttgtc gctgttgccg cgcttacggc ctttactgtt ctgcatgtgt tgcttattgt
      541 atggcaaatt gcgccaatat ttccatctgt atggttatct tttggaattc aagtcccgaa
      601 ggttcaaaat tagaatctgc cattgaagct tccatcattc ggcttttcat catcataggt
      661 tctgcataat ttgtgccctc aacttctttt atcagtaata ccttgccaac cttttctcca
      721 attccttcac aaagataggt ggccttagct ttggctgcct gtaatgcttc tgtttttact
      781 tgttttttaa actcatccat cttactgtgt gaagtggaac tgatgtacat gttctgcgtg
      841 cctttgtcat caagctgact cattaatctt tcaatctgct cacctgaatt aaacttaatt
      901 atataacttg aagaagcaac aaaatcggga gttttctttt tgcgctgcca gtaccaacca
      961 ctgttattgt agccattcat tccttgtatt gaaatatttt ctttagctat accggctttt
     1021 gtgcatgctg caagaaaatt ttttcgcacc tcatttatat ctgtcttctt cttattctct
     1081 gtatattcct gaatggttat gacaacaaaa atttcatcag gtattacttt catttcagca
     1141 gttcctgtga cttcaatttt tcttgtttca ctcatattga ccggggtttg tgaatgtgcc
     1201 attttaccgt ttaacatcat tccgataatg gctgtcagtg ctattagttt tgtttgcatt
     1261 ttaattgttt tccaatgtgg atgcaaagat aggtttaatt ccacagtgtg gctttgattc
     1321 gttcatttta agtttaatta ttcagatatt tgcagccgca aaaaatcaga ttatgaaagc
     1381 aaaaactgct aaagagagtt taaccataca aactgagatt gttcttccta acgacaccaa
     1441 cgtacttggc aatctgatgg gaggccgcct gctgcattgg atggatgttt gttctgccgt
     1501 agctgcacac cgtcattgtg gacgtgtagt ggttacagct tctgtaaaca atgtatcgtt
     1561 taatcaacct atccgtttgg gcgatattgt taccattcag gcaaaagtat cgcgctcctt
     1621 tacttcaagc atggaagttt ttatggaagt ttgggttgaa cgtaatgcca caggagataa
     1681 aataaagtgc aatgaagcca tctacacctt tgtagctgtt gaccaacaag gcagccccat
     1741 tgcagtgcct gaattgattg ctgaaagcga agaagaaaaa aaacgttttg atggtgcatt
     1801 aagacgtaag caacttgcct taatccttgc aggaaaaatg aaacctgccg atgcaactga
     1861 actgaaagca ctattctttt aaagctacaa agctctattt tgcatcttct tgtacatgtc
     1921 gtgaataaat gactttctga ttttacttgt taaattctgt caatagtttc tgttagtttt
     1981 gctctatatg gaaaaatcaa ttcagactta caataacagt caatcacagg aagacaaaaa
     2041 aatttgcgat ttattggctg attcaattga cacagtatta aaaacagcag aatgtaaaat
     2101 atggcatgca cacccggttt ggtttattga tgaaaatcca atagttggat acagtaaaca
     2161 aaagctaggc tggaggctta tgttttggag tggaattgat tttgaagaag aaggtttgaa
     2221 tgtgcatggc aaaaagttta aagatgcttc tgtattttat acttcaattg aacaaataaa
     2281 tattaaggat ttaaagcggt ggctaaaaaa atctataact attcaatggg attataaaaa
     2341 cctgataaaa agaaaaggta aattagtccg tttaaaataa ttgatatgaa tagcacagaa
     2401 aaaaatattg agcgtgtagc aaaaatgagt tttgcttcta tatatccttt atatatttct
     2461 aagattcaaa aaaagaatcg cacttcagag gaattgaatc aggttattac atggctaacg
     2521 ggttatagtg ttaaagaatt acaaaaacat ttagacaata aaacaacctt tcaggaattt
     2581 tttcagcagg ccaaaattaa tccaaatgca agtaaaatta caggcaccat ttgcggttat
     2641 aaaatcgagg aaatcgaaaa tccttttatg aaaaaagtca ggtacttaga taagttagtg
     2701 gacgagcttg caaaaggtca cccactaact aagattttac ggtcttaaaa tattatttca
     2761 tcaacgtcct aacacagtca cttttgcttt tcggtttgaa gcatttttag tatcgtacca
     2821 aaaaacaatt ttttcaatga tgcgtttgtt tcctttcagg tcaataactc tgctttgact
     2881 gttcttatca aaactatgac gcatatcaat ttcctgttcg cctccattct cgaagtgaac
     2941 aataacttta tgcatattca aagcacctcc tctgacaaca aatttcagtt tctgaaaaac
     3001 accgtcacgc aactgcacat ctaacacatc tttgtctaac tgatagtcca ctgtttttgt
     3061 gcctaagatg cgccaattgt caggcttaaa agataaactt accatagtta ccataattaa
     3121 taagcccatt actacagaaa ttctttgttt catattttta aaattaatat tgttgctttt
     3181 tcaaactaat agactatttt atgaaataat ggtttaatct gctttatgaa ttttttctgt
     3241 ctctttgtaa gtaaactgac ttaaaacaaa tacttaatct acgttattgt aaaaaggaag
     3301 ggctgataat aattttgcac tataaatata cattatcatg gaaaaaagat ctgtagaaat
     3361 agttgttagt ccacgtgaac cacattttgt aggtgatggc ttcagagtac ataatttcat
     3421 accaagtggt tatcgtcttg acatgcaaag aatgaatcca ttcatcatgt tagattataa
     3481 ttctaaattt cactttgacc ccagtgatac tcctaagggt gttggagtac atccgcatcg
     3541 tggatttgaa acagttacca ttgcctataa aggtaaggta gaacatcatg acagcagcgg
     3601 tggtggcgga gtaattggtg aaggagatgt tcaatggatg actgcagcca gtggtgtttt
     3661 gcataaagaa tttcatgaga aagaatggag caaaacaggt ggcgaatttc agatggtaca
     3721 gttgtgggtg aaccttcctg caaaagacaa gatgagtaag ccaaaatatc aggcattaga
     3781 aaattcaaaa atcaatcgtt ttaaatctga tgataaaaaa acagaaattg aaattattgc
     3841 aggagaatat aaaggagtta aaggaagtgc ctctactttt acaccattgc atctttacaa
     3901 tgttaagatg gcaaaagatg ctaaaacaga aatgaatctg cctaaaaatt tcaacacagc
     3961 gttattggtt attgaaggaa gcattactat aaatggaacc gaaaaagtag ataccaatca
     4021 ttttgctctt atggataatg atggtgaaag ttttacaatt gattcaaacg aaaatgcagt
     4081 ttatctggtg cttagtggcg aaccgattaa tgaaccaata gcagcacatg gcccttttgt
     4141 aatgaataca agacaggaat tagtgcaggc ttttgaagat tttaatcagg gacgatttgg
     4201 cactttgcaa gaataatttt tttctatttc agaataaaat tcaatcctga ttctcggctt
     4261 ctctacattc aatttcaaag cggttattga tatatccatg acgaattgat tcaattatat
     4321 agagaaaaat aaaccgtaaa aaaccgtagc gtttaaattg ttcaatgtgc ttgagttcat
     4381 gcttcatcca tttggaatca ctctgaaaag ttttaactga tgcaccatgc aggtaaattg
     4441 tttttccaat gactattgcc attgttttat agcctaaaaa acgggctgca atccatgcta
     4501 tcagtgcatt ctctttaatc cggaaattca tttactgggg ttactatctg attacgttta
     4561 catttccaac ataaaaatgt tgcttattga ataaatcgcg aacggttgct ttatatacgt
     4621 aaacaccttg ttgtactttg tttcctgaat aggttccatc ccatccttta ctcaaatcat
     4681 ttgatgtaaa tagtaactgg ccccagcgat taaaaacctg cacattaaat tctgtaatgc
     4741 caataccaaa agcattccat gtttcattaa agccatcttg gtttggagtg aaagagttag
     4801 gcatgtaaaa agtaaagtca tttttaattt ctatgctgat taatgctgtg tccttgcaac
     4861 catctgcact ttcaacatat tgaataatgg tatagtgttc aggttgctta taccaatgtg
     4921 aaggattttg ctctgttgta tttgtggtat caccaaaatc atagaaccat gatactgtat
     4981 tccatgactg atctttaatc tcaatcagtg gatgtaaaat tgtattgctt aatggctctg
     5041 caataaatgc agcttcaggt tttgcaaaaa catgaatgta atcctgtcgt aaaaaggtat
     5101 cgcgacatcc taaatttgtt tcaatataca atacaggact gaaatatcct gcctgatcat
     5161 aaacaattgc tgcaggattt gttaaagtat ctgatgcacc attatcaaag ttccaattcc
     5221 acatggtaat taacccttca ttggcaactg agctatcaga aaataaaacc aaaaggggct
     5281 gacatccttc aacagtatct gcaataaatc ccgctgtggg caactgataa acacgataaa
     5341 tatgttttat actatcaaca cattgattgg ctgttgtaac tactaaagta atttcataat
     5401 caccggtact taaatatgta taggtaggat tagctacagc agcagaagag ccatcaccaa
     5461 aactccactg attggaaaca atactccctg caacaacact acttgaatta ttaaaaatca
     5521 ttggttgatc tttacaaaca tttactgatg aaaatgctgc ctgcggttta ggtgcaacag
     5581 taacggaatc ggcaactgtg tcacgacagc cgaaactatt agttacaatg agctctgtat
     5641 aataaacacc ataagcacca aaatcatgca caggactttg tagatttgat tgatcaccat
     5701 caccaaactg ccactgccaa atttgcaatg agtcatttac agtagaagac tgatcataga
     5761 atgcaacagg agctaactga catgcattgc tatgtgtaaa tgaggctata ggtttatcat
     5821 gaattactat ggcaatggtc gtatcattct tacaaccatt acttcctgta acctcaaggt
     5881 ttatattaaa gttgccatca ctttgataaa agtgtgttgg tgaaaaaatt gaatcacttg
     5941 tattatcacc ccattgccat aaagtgtttg taatgtttcc tcccgataat gctgattgat
     6001 tgtttggcgt taatacagct tgctcacaaa tatcacttac agaaaagact gcatcaggta
     6061 aatgattaac taaaacagga acagaaacag aatctgaaca accatttgta gcccgaacaa
     6121 tcagtttcac aaaaatattt ccagacagtg aatatgtatg taccggtgat gctattgacg
     6181 cagtagcact gtcaccaaaa ttccattgcc accaattgat attatatcct gataaagccg
     6241 atgtatcctg aaactgtaca gggctgccaa cacatggatt ggcaaatgta aagccaactt
     6301 caggcaaagg cctgatgatt acattctttg taattgtctt actgcaaaag ccttcattct
     6361 gtactgtcag tgaaaccggg taaattgtat cctgtgcaaa aatatgtgac ggtgttggca
     6421 ttgtcgaagt agagccatca ccaaaattcc acgaccatgt aattaaacta tcggaaattg
     6481 gttgtgacaa gtcattaaaa actgtggcat tacccaaaca tacatgcggt gcgaaaaagt
     6541 tggcaacagg taaaggaaac acgcgaatag tgtcaagtgc tgtgtcagca catgcaaagt
     6601 ctgatattgc aaataattga acctgataat atccgtcagt tgtaaaagtg ttttgtccat
     6661 gctgagatat agaactgtgt ccatcgccaa aattccattg ccagcttaca atatttcctg
     6721 aggatattaa tgatgtattc tgaaaagttt cagctgcatt gacacaggca cttgatgaaa
     6781 caaattgtgc taatggcttt ggtcgcacgg taatatttcg agagattgtt gctttacagt
     6841 tattgctacc cgttactgtt aatgtaaccg gttgaactcc gtaatttgaa aatgcaaact
     6901 gcgggttggc agtattggta gtgccacttc caaaatccca aagccatcct gaaatatttc
     6961 catttatcaa tactgaagta tcataaaaac ttcccggtgc tgactgacac atttcatccc
     7021 atcggaatcc ggcaacaggc aagggcttaa tataaatctg tctgtaaaca gtgtcgctgc
     7081 aaccaagtgt attacctaca attaatccaa tctgcacaaa acctgtatct gcataaacat
     7141 gaaaaacact atctgttcct gtgaggaaag taccatcgcc aaaatcccat ttcaagtaat
     7201 tgatacttcc atttgcagat gttgaaacac tgttcagatt gatgctgtca ttaacacaac
     7261 ttgaagagtt tgtaaatgat ggcacaggct tgccgctaac actaacttca tgcaatactg
     7321 tatgtgtaca accattgttg ctggtaacag ttaaatatgt attgtaactt cccggtgctg
     7381 cataggcatg tgttgttgct gctgtagtgc cgtgtgcagt tccatcgcca aaatcccaat
     7441 cccatcctgt tatggtacct gaagatatag ttgatgtact ggaaaatgat agcggcacta
     7501 aggcacatcc tgatggtgca gaaaatttag ctacaggtgt tattgaagtt gaaatattat
     7561 ttacaacact atcaatgcaa ccatttgaag caccaacaat actcatcact gcgtaagtgc
     7621 cggcaccggt gaaagtgtga ttaacattct gacccgctgt ttgcgcactg ccatcaccaa
     7681 aattccaaat ccacgaactt atggtttcac cggcaggtgc aacagcagta ctgttaaact
     7741 gagcctgctg tccgggacaa acgggcaatg aggtaaatga tgctacagga gaaggtttaa
     7801 ctgtaatact acgtgccata gaatcgcggc aaccattatc gcctaaaaca accaatgata
     7861 tattaaaact tcctgtaccg ttatattggt gaaatggatt ttgcaaagtt gaatttgttc
     7921 catcaccaaa gtaccacagc caggatgaaa tactgccacc tgtaagactt gatgcatcat
     7981 taaatgctgt aaactgcgat tcacatgcgc cactgttagt aaaagctgct gttggcaaac
     8041 ttgccatagc cacacctttg gtaatggtat cagtgcaacc attgctgcct gtaataatca
     8101 gttgaactgc gtaattgccg ccaccgttgt aaacatgtac aggatttgca gtattatcta
     8161 atggtgagcc atctccaaaa tcccattgcc atgcagttat ggtgccaccg gtcagttgag
     8221 aaacatcagt aaacgtaacg gtattattca tacactgcac actgaatgta aatcctgcta
     8281 ctggatcagg cattggtgcc acaggtttta caatagtatc tacacagcca ttatttcctg
     8341 taacaataag tgaaacatca taaggtccca taccactgta aacatgtgtt gggtcaggta
     8401 ctgctgatga aggaagccca tcgccaaaca accattcata acttgtaatt gttcctgatg
     8461 gaaaacttgc attctgatta aaagatatag cttgacctgg acatgcaggt gtaaaattaa
     8521 agtctgaaac aggcgatggc aaaatactta ttgatttagt aattgatttt gtacaacctc
     8581 ctgcattggt tgccgtaagt gttacattat aatttcctga tgccggaaat tgatgccacg
     8641 gattctgcat tgtcgaagtg gtgccgtcac caaaatccca caaccattga ttcaccggtg
     8701 aacctgaaag aacataagat gaatcgaaaa actgagttcc actttcgcag cccggattaa
     8761 agttaaagtt agcaaacagt tgtgtaacgg caataacctg attcaatgtt accacacatc
     8821 ctgtcacaga tgtcatctga acagaaaagg tagcaccaat gacggggttg ttgatagtga
     8881 ttgtttgtgt tgttgcacca ttactccaca ggtaggatgc aaatccggga ggtgctatca
     8941 gtagtgctgt tgagctaccg gggcagtaat cgcttgtaac agtcatgctg ctgcaatagg
     9001 catcaacata ggcataacca aagtgtccgc ctaaagagca atcacctgta gaaaattcaa
     9061 tggtaacagt ttgacccatg taggatgaaa gttcaatacc tactgttgtc catgtcttaa
     9121 aatgaatatc gctgcctata ttgggatcga gccaggtttg aaaaccggga atgccggcac
     9181 ctgaaaccac attgtaagtt ccacaaccaa caggattacc tgcctgatca aatacacgaa
     9241 tctcaaaacg tggttgttcc gtagggtcgt gcacagggtc ttctaaaaca actgcgtagc
     9301 gataaataaa taattctgtt tgtggagtaa cctgaaagga ataagaaagt tgctctgctt
     9361 gtgagcctgc ctgatcatta cctaaacgtg cagagtaagt tccaccgggc gcaacaacag
     9421 gaatctgatt gttagagtga ggatcaaatc ctgtacctga cataatagta tgctgtccgg
     9481 taacaatacc gtttgaaaac atactgatag ggcagcatga acctgttttt ccagtccagc
     9541 ccacgaagtt tccgagttca aaatcaagat tggtacaact acttggctgc tgcccataaa
     9601 gcaaattgtt tatagacaac gttagaaaaa caacaataaa accaaatatc tttttcatga
     9661 ccagatatat cttgtttagt tggccggttc tttaattggg taaaagtaac agattttttt
     9721 aaaaaaagga acttttattt taaatttttt agctctgaaa gcaaaagttt ctgttcaaat
     9781 attttcaaac tactacttct aagtgttctg gtttatacaa acaaagaatc ctgcattaag
     9841 caggattctt tgtaaatatt tttatagata ctttatcaaa taacgcttac ttcttcctct
     9901 caaaatctcc tgcctgcaca aaagaaactt tattcggatt aatccatttt ttcattgctg
     9961 catttacatc ttcaactttc aaggctttga ttttattttc aaattcctca tcccatttta
    10021 tggagcgacc gagataggta tagctgttaa gtttgctgca caactctctg tcctgagagc
    10081 ggcttactga acgtgcctgt attaatcctt tcacagcatc tgttagttct gcattggtaa
    10141 atccttcgtt caacattttc tgaacctctt ctttccatgc tgcaatcagt ttatctgagt
    10201 tatcgggatt gtaaattgca taagataaaa atccacctga ctcatctaaa gaacttgcct
    10261 gcaattgtga gcctacacca tagctgatgc cttctttctg cctgatgcgt gttgcaaggc
    10321 gtgaattcaa gaaacctccg cccaacatat aatttccaac catcagtgcc ggataatctg
    10381 cattgtcatc tttcaatggt aagttcattc ctgccaccat caaagcattc gctttgtcgg
    10441 gggtattgat tttctcgttt acaggagtaa catcaaaata tggttcttta gcacgctcat
    10501 ataaaaccgg tgaaccccat ttatcaagta aattgttcag agaaataatt aatgatggct
    10561 catcaaaatc accaacaatt gctgctgtag catttgaagt ggtataaaac ctctcataaa
    10621 aattctttac atcagccaat gtaagcttat tcaatgcttc tttttcttcg tcaaaactca
    10681 tttcataacg gaaatcgtct tttggataag gattcagtgt tttactatat accctgaatg
    10741 caattgcctg tggttcgctg cgtgagcttt caagatcaga aatttgttcc tcttttaact
    10801 tatcaaattc tgcctgtgca aatgcaggtt gtttaatgat ttcacctatc agatttagaa
    10861 taccgctgag gttttcttta gtagttgtta ccgatacatt aacagtttgt ccgttaccgt
    10921 taatgcctac attagactta agtttgtcaa tctcatcatt aatttgctga aatgatttat
    10981 tctttgttcc tctgcgcaac atcgaagcag tgaatgatgc agcagtacct ttattttgca
    11041 tcactgcagc atttcccatt ctcaatgtaa tgtttacact gacattgcca ccacgagtgg
    11101 tttttttcag taaggtatat tttgcaccac cggaaatagt tgcctgtttt attctgttgc
    11161 tgatgtttgt tggcgaagga tcaaatgctt ctgcttctgc caacgctttt ttgcctttat
    11221 aatctttaac caatgcatta acatcgggtg atgcaggaat cacagctctg tccggtgttg
    11281 catcaggaat aaaaagtcct gtagtacggt tgcttggttt aaaatactct gcagccactc
    11341 tgtttacatc ggcagtagta actttctcta atgcatcacg ataaataaaa tacaaacgcc
    11401 agtcgccttg tgcaataaat tcacttagtg aaagccctac cctatcgctg ttgttaaaca
    11461 tcagtgtaag ttgtttgaga atggcattct tgccacgctc tgcctcttcg tttgtaatag
    11521 gatttttact caggttatca aaaatacctt tcatggtatt catgactgtg tcaagaggtt
    11581 tgtcttttaa tacttctgca tagaaatagg catatcccgg atctttaagc tgaaatgaat
    11641 agcccgatgt acttgtagct ttttttgttt ccaccaatgc tttatataat cttcccgaag
    11701 gttcgtttgt taatgcttct atcaaaactt caattgctgc atagtcggca tgagcacctg
    11761 cagggatatg atatccgcaa gaggcaacct gcacatcacc aactctgcgt aaaacaacat
    11821 tacgttcgcc atcctgtgca ggttctactg taaatgtctc ctgcaattta cgttcaggtt
    11881 taggaatagg actaaaatat tcgcttacta attgtaatgt tttttgctca tcaatttttc
    11941 cggcaatagt aagtactgca ttatccggct gatagtattt atgataaaat gctttcaggt
    12001 tttcaatagg aacacgctca atatcttctt ttgatccgat ggttgacttt ccatagttat
    12061 gccatagata agcagttgac attacacgct cttccaatac acttgaagga ctgttttctc
    12121 ctctttcaaa ttcatttcgc acaacagaaa attcagattc aagatctttt ttattgatga
    12181 atgaattaac cattctgtca ctttccaagc ttaatgccca acgaagattt tcttctgttg
    12241 cattaaatgt ttcaaaataa tttgtcctgt cataccaggt agttccattt ggtcttgcac
    12301 catgtgcagt aagttcctgc ggaatatttg tatggttggt agaaccttta aacaccatgt
    12361 gttctaataa atgcgccatt cctgattctc cataaccttc catgcgcgaa cctaccaagt
    12421 aagtaatgtt taccgtgatg gtagattttg atggatcggg aaacagtaat acacgtaagc
    12481 cattgctgag tttatattcc gtaattcctt ctacggaagt aactttaacc ggtgtagttg
    12541 ttgatttggc tttgtcttgt gcaaaaacat ttgctgataa caatgctgta atggcagcta
    12601 tcagaataaa ctgatttgtt tttcgtgatt tcataatgat ttgaattgaa ctaataagaa
    12661 aattctttta ccaaaggtag tttctgttgg tgagatattg ttgtatcatc cagttaaatt
    12721 gctgagtagt aaaaccacat ttgcaaccgg gagaatagta tgacatgatg ttgccgattt
    12781 gcggaacata ccaattgccg gaagggtcag gcaaataagg aagcatctga cagtctgcac
    12841 cgtttaatcc attagggtct gcaggtgtat cgcatatcag atcaccggct accagacaat
    12901 ttgtgccatt aattaattca actccattgg cagtttcgaa cgtatgataa agtccaaaaa
    12961 aatgtcccat ctcatgcgag aaagttttgc catcgcctac acaagattta ttcagaaaaa
    13021 tcatatcgcg tccacccgga aaatgggcat atccacaaac cgatgagttt gtattgtcgt
    13081 ataatgtgtt gctgaaataa acatttatag tatttggagt atagtataaa ttataaactt
    13141 cattttcatg cttcaatgca taaaaatcat tgtaattgta attgttgaca tacacaaact
    13201 cacagatgct gaacgacaaa cacataggtg caaagtcatt attcagaatt tgcaatgccg
    13261 acaaaattga tgcctgtgtg atgccatcgg cagctaaact atctcgaaaa atataggcag
    13321 tgatagaaag tgttttattc aaacagttta cagggaaaaa aagcccgcgt tgattttgtg
    13381 aagaatttaa attagctgct gcttcggctt gcattttagc tgtatcaatc acagtcccgc
    13441 acaagccttg tgcattgcta tccgatactg caaaaaggca ggaaaataaa atgacataaa
    13501 ttaaagttgc tgctttcatg caaaggctat acttttgagt gattgaaaat tcatcttacc
    13561 gcatacagac actacaaaca tataaatttt ttacatcgtt accgaaaagc cgtaaatttg
    13621 aaaacaaaaa ttaacatttc acttttttct tcacgcatct tgaaaagatt attctttttc
    13681 attcttttta taggcattat atcaaatgtc agtgcacaaa ttacagcgag caccacaagt
    13741 ggctgtgtgc ctttggtaaa tgttaatttt acctcgcctg cagggctgac aaatattgat
    13801 tgggattttg gtgatatgac ctcatcaaac ctgcctaatc cggtacatac tttttttaca
    13861 gcaggaagtt ttactgttgt ctgcacaggc ttgctctcag gaaatgcttt tattgattca
    13921 ataaaaataa atgtcttccg aaaaccattt acatcgctta cggcaacacc tccgctttca
    13981 ggttgtgttc cattaactgt tcattttaat gattcatctg ttggcggtgg tggtactgca
    14041 attgtttcga ggcagtgggc atttggtgat ggcgcaacca atgtaggaaa caattcttca
    14101 ccaacaaaca tttattctct tgccggaagt tttacagtct cgctaaaagt gactgatgcc
    14161 aatggctgcg acacttctat ttctgttcca aattatatta atacaagcat tcaacctaac
    14221 gctgtaatta caacagtgcc cagcccgcca ataggttgca caccgccatt tacagttgcc
    14281 ttttctgcct cagcatctac atcaaattct acaacgggaa cagggcttac ctacttttgg
    14341 aattttggtg gtggaaatac atcaacggtt gtaacacctc cttcaataaa ctatcccacg
    14401 agtggaactt ataacatttc acttacggtt actgataaca acaattgtcc taagacagtt
    14461 actactcagg ttgtcattaa tcctccacta gcatcattct ttgccgttgg tgcagttaat
    14521 gatacggttt gtcctacagt aactttcaga aatcaatcaa ttggtaccaa tccattctat
    14581 aactacggtg atggaacttc aggcgttgac agtattcata cctatgcacc gggaacctat
    14641 caggtaacac ttcaggtatt aaatggtatt tgtactgacg atacaaccat tactattcat
    14701 gtagaaaatg ttgttgccaa ctttacctca acaccaagtt acagctgcag ttggccattt
    14761 actgttcaat ataccaatat aacttctggt gcaggtacct atcaatggat ttttggtgat
    14821 ggcaccacat caaatcttgc aaacccaagt catacctttt atcgtcctga tacaaaccaa
    14881 tacaccattt atgattatta ctactacacc gatcagctga cttttacgag tgtacatggt
    14941 tgttctgcca ctgttgcaaa acaaaaaaac gacactgtgt ttagtatcac tgctcgtttt
    15001 atgcctgata aatcaattgg ctgtgctcca atgactgtta ctttttctga ttcgagtcgt
    15061 tctcgtgaga ctattgtaaa ttacacctat ttatttggcg atggcacaca aactacagga
    15121 agcaatggtg tggttacaca cacctacaac acaccgggta tttactatgt gaggttagta
    15181 gttacaaatt ctgcaggatg caaagacact tcttttataa ttccgattta tgtaggttct
    15241 gctgttacaa catcatttac tgttgttcct acaactatat gtgcatacac acctgtacag
    15301 tttaatgttc tgaat
//
LOCUS       DAOOOL010000055        15191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_327_length_15191_cov_62.995706, whole genome shotgun sequence.
ACCESSION   DAOOOL010000055 DAOOOL010000000
VERSION     DAOOOL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_15191_cov_62.995706"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKU82_06105"
     CDS             complement(<1..523)
                     /locus_tag="PKU82_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82483.1"
                     /translation="MKWIKRLFLVTLALSLTISACKKEKDDSPSPTPPTPIPPTPSGN
                     TPKNLGWNGGDNPNSVPTSVTNTPVFNSGTLPTSVSLLPQFPPIGDQGQYGTCVAWAA
                     GYNMKTVIDAIQQNLNASDLTNTNKQTSPKDVFLAIPDAKKGQNCSGTNFSDAMDLLL
                     NRGAATMQTVPYTN"
     gene            678..1865
                     /locus_tag="PKU82_06110"
     CDS             678..1865
                     /locus_tag="PKU82_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOZ82484.1"
                     /translation="MNIDKIKQLAEIAFNIVLADRRHLHKNPELSFKEFQTQQYVWER
                     LDEIGISNKKKIAETGIIAMIEGKNPSAKIIALRADMDALPIQETNKTDYVSQNAGVM
                     HACGHDVHTSSLLGVASVLQQLKSEFSGTVKLLFQPGEEKLPGGASLMIKEGALKNPS
                     VSGILGQHVMPMIETGKVGFRSGVYMASTDEIYVTVKGKGGHGAIPHQNIDPVLIASH
                     IIVALQQIVSRNANPIMPSVLSFGKVMANGATNVIPDSVYLEGTFRTLDEKWRSDAHT
                     KMIRMANAIAEGMGGSCEFKIIRGYPALVNNPQLTASARKNAEAYLGKENVLDLDIWM
                     AAEDFAFYSQETNACFYRLGTRNEANGITSSVHTSTFDIDEKALLTGVGLMSYLALCE
                     LEQ"
     gene            complement(1907..4774)
                     /locus_tag="PKU82_06115"
     CDS             complement(1907..4774)
                     /locus_tag="PKU82_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOZ82485.1"
                     /translation="MKNNLYSKLGCLLLLAPAFSFAQLSFTNSNSRLASPAFHSGCPV
                     SVVDWNNDGLDDIIRLDNGKHCYVDVHKTNQQYQRIFLGTFSGSTWAMAVADLDKNGY
                     KDVVAGGAFGSVEILKTNNSGTGATLSSLSNSNFFLQNLTMADFDNDGWIDIFCCDDN
                     AASHIYLNNGSGGFGAQSNIINFNLPNGSDNSGNYGSVWTDFDNDGDLDLYIAKCRQS
                     VNNPSDPRRINVLFVNNGNGTFTENAAAYNINIGWQTWTAAFGDIDNDGDLDLMITNH
                     DHNSQIFQNDGTGVYTDITSSTGFTVSDITPIESVMEDFDNDGFVDIFVTGSDSRFWR
                     NNGNSTFTLVENLFDNNDMESFAIGDANHDGWIDVMASYANIYTIPSNIDDVLWINNG
                     SKQNIIDGVAGLPTNNFFNVVLEGNTTNKDAIGARALIYGTWGVQLREVRAGESYGTN
                     NSAILHFGLGTATAIDSMKVIWPSGVSQTISNPSINQFLSIKENDCVSPEAIVTASGP
                     LVLCTSGQALTLTAPAGFNYLWSDSSTAQSITITQTGEYNVTISQPGNNCSATSATMV
                     VIPSPNQTPAITALGETVFCNGGSVQIQGPIGFSSYLWSNGDTTQTATVTTNGPVTLT
                     IQGICQSFTSDPINVTVNTVTDPIANDVILSAAGSATLNATVGNNISWYATNAGGTPL
                     ATGPSYTTPVLTANTTYYMQATDTFGGGIYAVGLPTWSGSGSGYSGNTTNATTEFNVT
                     KNCTLKSVKVFTNVAGIRRIELKDASSTVINYVDVNVSPDSQVVTLNFPLTPGMGYTL
                     GTNTTTNQNNLGTDSPKFKRNNSGVNYPYTLSDALSITNSSNGGQYLYYFYDWQVEKE
                     SWECVSNMVPVNVYITTGIKENDRSILSAYPNPVSDKLNISNPFGTVKATLFDATSRV
                     VGSYVLQNGENIFNIESLNAGVYQLQVNHDGEISNIRLVKN"
     gene            complement(4834..6765)
                     /locus_tag="PKU82_06120"
     CDS             complement(4834..6765)
                     /locus_tag="PKU82_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82486.1"
                     /translation="MKNKIIVLFLISVAIIGISAIPFQPKKAMAYHSESEIAFFKNHS
                     MTPIAPGEFFLTPDNCKGCHGFDTLGIANVDANGQDVNLHDDWETSMMGLSAVDPFWR
                     AKVSHETMVNPAHATELQNLCITCHAPAGHYSAIYKGQQHYVMSDLLNDSLGLSGVSC
                     TACHAIGDSSSLGQLFTGQIPYDTNKVLFGPFISPVAGPMQLYVGMIPTYSPHVSEGK
                     FCSPCHTLLSNTVDLNGIPTGGTFIEQATYHEWLNSTYPSQATVCQSCHMPQIEDAVI
                     LANGNVGLPGRTPFNLHQFAGANSFMVNLIKQNKTALGVSAPDYNFDSTLAAITHMLT
                     TQSLDVQASLAAITSDTAFIDVDLTNKAGHKFPSGYPSRRAVLQMIVTKANGDTLFAS
                     GLFDANYEMQNVASPFEQHHDIINSQTQNQIYEMVMGDVNGTVTTVLERAASHLKDNR
                     IPPAGFSTLHYAYDTCKIVGSALTDVDFNKNGATQGTGHDIVHYHIPLNNYSGTINVY
                     TAVYYQTLPPAFLSDMSNYSSPEINTFLTMYANADKSPVKVDADTLLNISTGIKENIL
                     TTINISPNPTRDGKVLLSGIGYNSFQLEVFDQNGKKINVILTRKNNSIEVQLPTAKGI
                     YFIRLNVNGQVKSYKILRI"
     gene            complement(6928..9396)
                     /locus_tag="PKU82_06125"
     CDS             complement(6928..9396)
                     /locus_tag="PKU82_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOZ82487.1"
                     /translation="MNKYLRYFVFLLLTCLALLLIAMGLSVLLQDKIKQSVIEEINKS
                     LNTPASVSEIDFSVLRFFPNASLTLKNVVIKGNPWQDTKRPLLKAGKIDLVLSITSIF
                     SSTIQLKKIEISDAELNILTNENGQKNDQIFKQGNDNNSFAAEFKKVILKNVNLSIEN
                     KPSNFILQTHLNKSLASGNFKDKTFELKTETDLFAEIIQHENISYIKQKPIQLNGVVS
                     VDLRNDIYAFNDLKIQLANLNINAKGKIEKRNETFLDLKFTATKANASELLSILPSNW
                     VAPQILNYRYHGNINFDTHINGVASSKQSPTIEINFGTNKTDIIPDNENYALKNVALK
                     GFYSNRKSGKNISVLSLKNISARIEGKPIIGEVYLENLKNPYFNIQLDADISLASLSR
                     YLPADFTEEQQGSVSLHGRISGQADKKDTYRSNGTLTLNNLKFKLKKRNIAVNSISGK
                     INFNNADVKVESLKILAGQSDVVINANILNFYNYIFRDNQTITINGIVESHFLDAGEL
                     LAGNQSTDTSDFDLPAYLNLNASLSADVVKFRKFNAKDLSGNISLQDKILTAKQLSFN
                     AMEGHIALDGTINTNFKDEVHISSNAKIESINIERLFFETGSFGQQVLTEKNIRGTLT
                     ASVQLATYWTKQLEVKQSRTIASADLSINDGALIDFKPMLKLSRFVKGSDLQNVRFSK
                     LQNTIRIHEQKILIPTMDIRSTAMNMTISGTHGFDNFVDYKIQMKLSQLLGKKVRQQN
                     TEFGTIEDDGSGGLNLFLTMKGPLDNPKFSYDRKGVEKKITESVKSGKDDFLKIIKEE
                     ISGKKPPKEETPKKQKELQIDYDE"
     gene            complement(9590..10192)
                     /locus_tag="PKU82_06130"
     CDS             complement(9590..10192)
                     /locus_tag="PKU82_06130"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOZ82488.1"
                     /translation="MSFQLPPLPYAPDALEPHIDTRTMEIHHDKHHQAYVTNLNNAIA
                     GTDAEKMSIEDICKNISKLPMAVRNNGGGHYNHSLFWTIMAPNAGGAPKGKLADAINS
                     AFGSFDEFKTKFNQAAATRFGSGWAWLIKNAAGKLEVCSTPNQDNPLMDVAEVKGTPL
                     LGIDVWEHAYYLHYQNRRPDYCTAFWNVVNWDEVSNRFEK"
     gene            10421..10693
                     /locus_tag="PKU82_06135"
     CDS             10421..10693
                     /locus_tag="PKU82_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82489.1"
                     /translation="MKKVLALVMAAGMFSIVACGPSAEEKQKMEETAKAAQDSIAAAA
                     QQAASAAEQAATDAAQVATDAAQTVDSTAGAVVNQATEAVKEAATK"
     gene            complement(10744..11868)
                     /gene="dprA"
                     /locus_tag="PKU82_06140"
     CDS             complement(10744..11868)
                     /gene="dprA"
                     /locus_tag="PKU82_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOZ82490.1"
                     /translation="MENNLTTDVLQYQLALTLLPGVGDITAKNLVGYCGGAEAIFKEK
                     QQALLKIPNVGQIVADKIKNGKEQSLKRAEEEIRFIEKNKIKPLFFTSDQYPKRLKAC
                     NDSPIMIFVKGNGNLNPEKCIAFVGTRKASDYGKTTTEKLIADLAETDGMMIVSGLAF
                     GIDITAHKASLKCKIPTAGIVAHGLDTLYPAEHRNTAEKMQAEGAVISEFMSKTNADK
                     ENFPKRNRIIAGMADAVVVVESGIKGGSLITADIANSYNRDVFAIPGQVDKEYSKGCN
                     ALIRDNKANLIQSAADIEYFMGWDKTDVKSIPKQARLFEDLNPEEKQLVDFLKENSKV
                     DIDTICFRSGVKLNRIPALLLNLELKGVIKNYPGKVYDLI"
     gene            11939..14206
                     /locus_tag="PKU82_06145"
     CDS             11939..14206
                     /locus_tag="PKU82_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ82491.1"
                     /translation="MFSQRITLFYFFFVLGIYSVNAQQQRVSGKVTDGEGELPGVAVY
                     ADESHATTSDAKGEFVLMLDKGSWTLKAKLLGYETFSKVILVGDSNYVQIHLIPLSKQ
                     LQSVVVSAGKFEQKLEEVTVTMETIKPKSFETTGDNNVETALEHVTGLNVNDGQANIR
                     GGSGFSYGAGSRVLVLVDDMPMLSADANDAKWSFLPIENLEQIEIIKGASSALYGSSA
                     MNGVINFRTGYAKSEPVTKIVQYNGIYDSPRRKEIKWWGKETQMLSGANMFHAQKFGQ
                     WDFVAGGHYLLDEGFRKGETEKRARVNINTRYRFPKIEGLAVGVNANYMESFGGLFII
                     WNDDTTGAYIPSGGVNDSTSTLSNYHTVRYNIDPFITYHSKKGFSAKWRSRYFRTLNY
                     NDTDQEAFADLYYSEVQLQQRFEKWNLVLTGGGVITDGGVRSDLYGSRKTYNKALYAQ
                     ADKKFFDRLNLSLGWRWEQFRFRYSKAESKPVIRAGANLRLLRATYLRSSYGQGYRFP
                     SIAEKYVKTRVGSIVIYPNDSIFSESGWSSEIGLRQLFAVGKQFSGYVDASVFRSEYK
                     NMLEFAFGSYGDPFKDPLFGLGFKSLNIGNTRIDGMEFELGVQATVLKKINLQFGGGY
                     TYIDPRLTDFDSLKASTGTITYNFLKYRYRNLFKGYGSIAYGKVSLAVNCRYNSFMEN
                     IDVVFNELLPGVKRYRAEHDKGDWIYDANISYKLNSQIALSVISRNLFNHEYSGIPSQ
                     LEAPRMWLFQFVITP"
     gene            complement(14252..14920)
                     /locus_tag="PKU82_06150"
     CDS             complement(14252..14920)
                     /locus_tag="PKU82_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82492.1"
                     /translation="MNKTIPDKPLRVIIVDDNKNMRESVALLLQSTPSLLLIAEFDSG
                     EECIKQVDTLKPDVILMDIDMPKLSGIETVKIIRKQFPGLPILMLTGFEDDDKVFDSI
                     CAGANGYILKNTSMELLIHHIHEVYTGGAPMTPVIARKVLTRFAKTQVTETKNEDYNL
                     SKREMEVLELLVKGKSYKTIATELKVSYETVHTHIRKIYHKLHVKSIGEAVSKTIKNK
                     ILNS"
     gene            complement(14969..>15191)
                     /locus_tag="PKU82_06155"
     CDS             complement(14969..>15191)
                     /locus_tag="PKU82_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ82493.1"
                     /translation="IHLSRNRGMTFLKIKDNGCGIDMANNHSQTNGNGGNGLKNMASR
                     AKDLNAEFQINSAPGKGTEITISLKQHAI"
BASE COUNT         4648 a         2634 c         2907 g         5002 t
ORIGIN      
        1 gattggtata tggaactgtt tgcatagtag ctgcgccacg atttaataat aaatccattg
       61 catcagaaaa gtttgtacca ctgcaattct gacctttttt tgcatccggt atagcaagaa
      121 aaacatcctt aggactcgtt tgcttgtttg tattcgtcag gtcgcttgca ttaagatttt
      181 gctggattgc atcaataaca gttttcatat tgtaacccgc agcccatgct acacaggttc
      241 catactgacc ctgatcacca attggaggaa actgtggtaa caaactaact gatgttggta
      301 atgttccact attaaataca ggggtattgg taaccgaggt aggcacagaa ttggggttat
      361 caccaccgtt ccatcctaaa ttttttggcg tattccccga tggtgttgga ggaataggag
      421 taggaggcgt aggtgaaggg ctatcatctt tttctttctt gcaagcacta attgtaagtg
      481 atagtgccaa tgttactaag aataatcttt taatccattt cattttttaa aattttaaat
      541 tgatgagtgc tacaaagtag aatataacaa actggattgt caatcaccaa atctggtgat
      601 ttttatagct gaaaacaata cttaatttga tgtataaaaa cgtaaaatat tgaacaataa
      661 agttttaatt ttgacacatg aatattgata aaataaagca acttgcagaa atagcgttta
      721 acatagtgct tgcagacaga cggcatttgc ataaaaatcc tgaactttca tttaaggaat
      781 ttcagacaca acagtatgtt tgggagagat tggatgaaat aggaataagt aataaaaaga
      841 aaatagcgga aaccggaatt attgcaatga ttgaaggtaa aaatccttcg gcaaaaatta
      901 ttgcactgcg tgctgatatg gatgcgcttc ccattcagga aaccaataaa acagattatg
      961 tttcacaaaa tgcaggtgtc atgcatgcat gtggacatga tgtgcatact tctagtcttc
     1021 tgggggttgc ttctgttttg caacaattaa agagtgaatt ttcaggaact gtaaaattat
     1081 tgtttcagcc cggagaagaa aaactccccg gtggtgcttc attaatgata aaagaagggg
     1141 cattaaaaaa tccttcggtt tcaggaattt taggacaaca tgtaatgcca atgattgaaa
     1201 ccggaaaagt aggtttcaga agtggtgttt atatggcaag tacagacgag atttatgtaa
     1261 ctgtaaaagg aaagggtgga catggagcta ttccgcatca gaatattgat ccggtgctca
     1321 tagcatcaca tataattgtt gcgttacaac agattgtaag tagaaatgca aaccccatta
     1381 tgccaagtgt tttgtcgttt ggaaaagtaa tggccaacgg agcaaccaat gttattcctg
     1441 acagtgttta tcttgaagga actttcagaa cacttgatga aaaatggcga agtgatgcac
     1501 atacaaaaat gattcgtatg gcaaatgcta ttgcagaagg tatgggtgga agttgtgaat
     1561 tcaaaattat tcgaggttat cctgcattag taaacaatcc gcaactgacg gcatcagcaa
     1621 gaaaaaatgc cgaagcttat ttggggaagg agaatgtttt agatcttgac atctggatgg
     1681 cagcagaaga ttttgcattt tattcgcagg aaaccaatgc ttgcttttac cgtttaggga
     1741 caagaaatga agccaatgga attacttcat cagtgcatac ttcaacattt gatattgatg
     1801 agaaagcatt gttgacaggt gtaggattga tgtcatactt ggcactttgc gaattagaac
     1861 aataaaaaaa ccgccccttt tcagaggcgg ctgctaagtt ttttgtttag tttttaacta
     1921 agcgtatatt acttatttca ccatcatgat ttacctgaag ttggtaaaca cctgcattga
     1981 gtgattcaat attaaaaata ttttctccat tttgtaaaac ataactacct actacacgcg
     2041 atgtagcatc aaacaatgtt gcctttacag ttccgaatgg attagaaata tttaatttat
     2101 cactaacagg atttggataa gcagataaaa tacttctgtc attttctttt ataccggtgg
     2161 tgatatacac attcacagga accatattgc taacacactc ccacgattct ttttctacct
     2221 gccagtcata gaagtaatat aagtactgcc caccattact tgagtttgta atgcttagcg
     2281 catcagataa agtatatgga tagtttacac cggagttgtt tcttttgaat ttaggtgaat
     2341 cagttccaag attattctga ttggttgtgg tatttgtgcc aagtgtataa cccattcccg
     2401 gagttaacgg gaagtttagt gttactacct gcgaatcagg agaaacgttt acatcaacat
     2461 aattgataac agtggatgag gcatctttta attcaattct tctgattccg gcaacattgg
     2521 taaatacttt gacagatttt aaagtacagt tttttgtaac attaaattct gttgtagcat
     2581 tggtagtatt tccagaatac cctgaaccag aacctgacca tgttggtaat cctactgcgt
     2641 aaatgccacc accgaaggtg tcagttgcct gcatatagta tgttgtgttc gcggtaagaa
     2701 caggtgtagt gtaacttggt cctgttgcta aaggtgtacc tcctgcattg gttgcatacc
     2761 atgaaatgtt attacccact gtagcattta atgtagcact tccggctgca gataaaataa
     2821 catcgtttgc tatcggatca gtaactgtat ttacagtaac attaattgga tcagaggtaa
     2881 atgactgaca aatgccctga atagtcaatg ttacaggccc attagttgtt acagtagctg
     2941 tttgtgttgt atcgccattt gaccaaaggt agctgctgaa tcctataggt ccctgaatct
     3001 gaacactacc tccattgcaa aacacagttt cgccaagcgc agtaatagca ggtgtctgat
     3061 ttggtgatgg aataacaacc attgtagctg atgtagcaga acaattgttt ccgggttgtg
     3121 agatagttac attgtattca cctgtttggg tgatagtaat actttgtgca gttgaactgt
     3181 cgctccataa gtaattaaat cctgcaggtg cagttaaagt taatgcctgg ccacttgtac
     3241 acaatactaa aggaccagat gcagtaacga ttgcctcagg tgaaacacaa tcattttctt
     3301 taattgataa aaattgatta attgaaggat tgcttattgt ttgacttaca ccacttggcc
     3361 agattacttt catagaatca atagccgtgg ctgtacctaa accaaaatgt aaaattgcac
     3421 tattattggt tccataactt tcacctgctc gcacttcgcg cagttgaact ccccacgttc
     3481 cataaatcag tgcacgcgca ccaatagcat ctttattggt agtatttcct tcaagcacca
     3541 cattaaaaaa attgttggtc ggtagtccgg caacaccatc aataatattt tgtttactac
     3601 cgttgttaat ccacaataca tcatcaatgt ttgaagggat ggtgtaaata tttgcataac
     3661 ttgccataac atcaatccag ccatcatggt ttgcatcacc aatagcaaat gattccatgt
     3721 cattgttatc aaataaattt tcaactaaag taaaagtgct gttaccatta tttctccaaa
     3781 agcgtgaatc gcttccggtt acgaaaatgt caacaaatcc atcattgtca aaatcttcca
     3841 ttacactttc aataggagtg atgtcagaaa cagtaaatcc tgttgatgat gtaatatcag
     3901 tataaacacc cgttccatca ttctgaaaaa tctgactgtt gtggtcgtga tttgtaatca
     3961 tcaaatcaag atcgccatca ttgtcaatat caccgaaagc cgctgtccaa gtttgccagc
     4021 caatattaat gttgtaagca gcagcatttt cagtaaaagt accattacca ttatttacaa
     4081 atagtacatt aatacgtcta gggtcggaag gattatttac agattgtctg catttcgcaa
     4141 tgtataaatc aaggtcgcca tcgttatcaa aatcggtcca cacacttccg tagtttcctg
     4201 aattatcaga accattgggt aggttaaagt tgataatatt tgactgtgca ccaaatccac
     4261 cggagccatt attcagataa atatgacttg ctgcattatc atcgcaacaa aaaatatcaa
     4321 tccaaccatc gttgtcaaaa tcggccatgg ttaaattctg caagaagaaa tttgaattgc
     4381 ttaatgaaga tagtgtagca ccggtaccac tattgtttgt ttttaatatt tcaacacttc
     4441 caaaagcgcc accggcaaca acatctttgt aaccattttt atctaagtcg gcaacagcca
     4501 ttgcccacgt acttcctgaa aatgtaccaa gaaatatgcg ttgatattgc tgattggttt
     4561 tatgcacatc aacatagcaa tgtttcccat tatctaaacg aataatgtcg tctaagccat
     4621 cattattcca gtcaactaca gaaaccggac agccactgtg aaatgctgga gatgccagtc
     4681 ttgagttact gttggtaaat gaaagttgtg caaaggagaa tgcaggtgct aggagcagca
     4741 ggcatcctaa ttttgagtaa agattatttt tcattttaaa caagtttctg atttaatgta
     4801 atttgttttt atttataaga gatgttatag tttctaaatt cgtaatattt tataagattt
     4861 aacctgacca ttcacattaa gtctgataaa gtaaatacct tttgctgtgg gcagttgaac
     4921 ttcgattgaa ttattttttc tggtaagaat aacattaatt tttttaccgt tttgatcaaa
     4981 cacttctaat tgaaatgaat tgtaacctat gcctgaaaga agaacttttc cgtctcttgt
     5041 tggattgggt gaaatattaa tggttgttaa aatattttct tttattcctg ttgagatatt
     5101 aagcaatgta tcagcatcaa cttttacagg acttttatct gcattggcat acatggtaag
     5161 aaatgtattt atttcagggg aagaataatt gctcatgtca cttaaaaatg caggtggcag
     5221 tgtttgataa taaacagcag tataaacatt aatggttcca ctgtaattgt ttaatggaat
     5281 atgataatga acaatgtcat gtcctgttcc ttgtgttgca ccatttttat taaaatctac
     5341 atctgttaaa gcgctaccaa caattttaca agtgtcataa gcataatgca aggttgaaaa
     5401 tccggcagga gggatgcggt tatctttcag atgtgaagct gcgcgttcaa gtacggtggt
     5461 tacggttccg tttacatcac ccattaccat ttcataaatc tgattttgtg tttgtgagtt
     5521 gatgatgtca tggtgttgct caaaaggtga agccacgttt tgcatttcat aattggcatc
     5581 aaacagtccg gaagcaaaca acgtatcgcc attagctttt gtaactatca tctgtagcac
     5641 agctcttctt gaaggatatc cacttgggaa tttatgccct gctttgtttg ttaggtcaac
     5701 atcaatgaat gctgtatctg aagtaatagc tgctaatgaa gcctgcacat ccaatgattg
     5761 tgttgtcagc atgtgtgtga tagctgcaag tgttgaatca aaattatagt caggtgctga
     5821 aacacccaat gcagttttgt tttgcttaat cagatttacc ataaacgagt tagcacctgc
     5881 aaactgatgc agattaaatg gagttcgtcc gggcaaacca acattgccat ttgccagtat
     5941 tacagcatct tcaatttgtg gcatgtggca actttgacag actgttgcct gtgatggata
     6001 agtggaatta agccattcat gataggttgc ctgctcaatg aaagtgcctc ctgtaggaat
     6061 accatttaaa tcaacagtgt tggaaagtaa tgtgtgacag ggtgaacaaa attttccttc
     6121 acttacatgt gggctgtagg taggtatcat gcctacataa agttgcatgg gtccggccac
     6181 aggagaaata aaaggtccaa atagaacttt attagtatca taaggaatct gaccagtaaa
     6241 tagctgacct aaactactgc tgtcaccaat agcatggcaa gctgtgcagc ttacacctga
     6301 aagaccaaga gagtcgttca acaaatcgga cataacataa tgttgttgtc ctttataaat
     6361 tgcgctatag tgccctgcag gtgcatgaca agtaatacat aaattctgta actcagttgc
     6421 atgtgccgga ttaaccatag tttcgtgact taccttggca cgccaaaaag ggtcaactgc
     6481 agaaagtccc atcattgagg tttcccagtc atcatgaagg ttaacatcct gcccgttggc
     6541 atcaacatta gcaattccta aagtatcaaa accatgacag cctttgcagt tatctggagt
     6601 taaaaaaaat tctccggggg caatcggtgt cattgaatga tttttaaaaa acgctatttc
     6661 agattcagaa tgataggcca tagccttttt tggttgaaaa ggaatagcgc taatacctat
     6721 aatggctact gaaatcagaa ataaaacgat tatcttgttt ttcatgcctg cattatcctc
     6781 aatatttttt tacggattga tggcaataat atcaattttt ttaatgtgct gcaacagaaa
     6841 tctttggcaa aacatcaaat tattaacgtc aataggtttg taatacaagt aaagagaatg
     6901 gtcggatgaa gaaataagaa cagtccatta ttcatcataa tcaatctgta actccttttg
     6961 ttttttggga gtttcttctt ttggtggttt cttgccactt atctcttctt tgataatttt
     7021 taaaaaatca tccttgccgg attttacaga ttctgtaatt tttttctcta ctcctttacg
     7081 gtcatagctg aatttggggt tatctaatgg ccctttcatg gtcagaaaaa gatttaaccc
     7141 accactgccg tcatcttcaa tggttccaaa ttctgtattt tgctgacgca cttttttgcc
     7201 gagtagctga gaaagtttca tttgtatttt atagtcaaca aaattgtcaa agccatgtgt
     7261 gccacttatg gtcatgttca ttgcagtgga gcgaatatcc attgtgggga taagtatttt
     7321 ttgttcatga attcgaattg tgttttgaag tttactgaaa cgaacatttt gcagatcgct
     7381 gccttttaca aaacgcgaaa gtttaagcat tggcttaaaa tcaataagag cgccatcgtt
     7441 aatacttagg tctgctgatg caatggtacg actttgtttt acttcaagtt gttttgtcca
     7501 ataggtagca agttgaacgg aagcagtcaa tgtgcctctg atattttttt ctgtaagcac
     7561 ttgctgacca aagcttcctg tttcaaaaaa aagtctttca atgtttattg attcaatttt
     7621 cgcattgctt gatatgtgta cttcgtcttt aaaatttgta ttaatagtgc catcaagagc
     7681 tatgtgacct tccatagcat taaatgaaag ttgttttgca gttagaatct tatcttgcag
     7741 tgatatgttt ccggaaaggt ctttagcgtt gaatttcctg aattttacta catctgcact
     7801 taacgatgca ttcagattaa gatatgcagg caaatcaaaa tcagaagtgt cggtagattg
     7861 attaccggcc agcaattcac cggcatctaa aaaatgactt tcaacaattc cgttaatagt
     7921 gatagtctga ttatcacgaa atatatagtt gtaaaaatta agaatatttg cattgatgac
     7981 aacatcactt tgcccggcaa gaatttttaa agattcaact tttacatcag cattgttaaa
     8041 attaatttta ccactgatgc tattaactgc aatatttctt tttttaagtt taaatttcag
     8101 attgtttaat gtcagtgtac cattggagcg ataagtatct ttcttgtctg cttgtccgct
     8161 aattctacca tgaagagaaa cagagccttg ttgttcttct gtaaagtcag caggtaaata
     8221 gcgacttaaa ctggcaaggc taatgtcagc atctaactgt atgttgaagt atggattttt
     8281 aagattttcg agataaactt cgccaataat tggtttgcct tcaattcttg cagaaatatt
     8341 tttcagactc aaaacactaa tattttttcc ggatttacga ttagaataaa atccttttag
     8401 tgcaacattc ttcaatgcat aattttcatt atcaggtata atgtcggttt tattagtgcc
     8461 aaagttaatt tcaatggttg gtgattgttt tgatgatgca acaccattta tatgtgtatc
     8521 aaagtttatg ttaccatgat agcgatagtt taaaatttgc ggtgcaaccc aattagatgg
     8581 taaaatagaa agtagttctg atgcattggc ttttgtagca gtaaatttaa gatcgagaaa
     8641 agtttcattt cttttttcaa tttttccttt tgcatttata tttaagttgg caagttgaat
     8701 ttttaaatca ttaaatgcat aaatgtcatt acgtaggtca accgaaacaa caccattaag
     8761 ttgtatgggc ttttgtttga tgtaagaaat attttcatgt tgaataattt cagcaaacaa
     8821 atctgtttct gttttaagtt caaatgtttt atccttaaaa ttacctgaag caaggctttt
     8881 gttcaggtga gtttgtaaga tgaaattaga tggtttattt tcaatactca gattaacatt
     8941 tttaagaatt acttttttga attcagcagc gaaggagttg ttgtcgtttc cctgtttgaa
     9001 aatctgatca tttttttgac cgttttcatt ggtcagaata tttaattcag catcagaaat
     9061 ctctatcttt ttcagctgta ttgttgaaga aaatatgctg gtaatgctaa gcaccaaatc
     9121 aatttttccg gcttttagca aagggcgttt tgtgtcttgc caaggattgc cttttatgac
     9181 tacattttta agtgtaagtg atgcatttgg aaaaaaacgt agtacggaaa aatcaatttc
     9241 actaacactt gcaggtgtgt tgaggctttt atttatttct tctattacag attgtttgat
     9301 tttatcttgt aataagactg aaagtcccat tgcaatgagc agtagtgcca agcatgtcaa
     9361 cagtaaaaaa acaaagtagc gcaaatactt attcatgtcc tcttaaaacg ttgcaacttt
     9421 tttaaattgt aataacgata aaaatgtatt ttgcaaagta attttattag cacatagtaa
     9481 actatgaaat aaggcgtttg ttttatggag ttttaaactt ttaatgtgca ataaaaaagg
     9541 ctgccttaat tcaattaagg tagcctttat aaattaatta atcgggaaat tatttctcga
     9601 aacgatttga cacttcgtcc cagttaacca cattccagaa agcagtacag taatcgggtc
     9661 tgcggttttg gtagtgcagg tagtaggcat gttcccaaac atcaatacct aaaagcggtg
     9721 taccttttac ttcagcaaca tccattaacg gattatcctg attgggggta gaacaaactt
     9781 caagtttacc ggcagcattt tttattaacc aggcccagcc tgatccaaaa cgagttgctg
     9841 cagcttgatt aaacttcgtt ttaaattcgt caaacgaacc aaaagcgctg tttatagcgt
     9901 cagctaattt tccttttggt gcaccacctg cattgggtgc cataatggtc caaaacagtg
     9961 agtgattgta gtgtccgcca ccattgttgc gtactgccat cggtagtttc gaaatatttt
    10021 tacaaatatc ttcaatgctc attttttctg catctgtacc tgcaatagca ttgtttaaat
    10081 tggttacata agcctgatgg tgcttgtcat ggtgaatttc catggtgcgt gtatcaatat
    10141 gtggctctaa cgcatcgggt gcgtatggta gtggagggag ttgaaaagac ataatatcta
    10201 atttaaatgt taagcaacaa aggtagtgaa atatgctacc tttgttgtca atcaagtact
    10261 attttcagaa gaaatacatt gatacagggg ttgttactct ccggctaaaa ttataaaaca
    10321 ctgataacca acgaataaaa atttaatata aaacttaata tgcagaggta aagcaattta
    10381 tatttgccgc tcaaatttca aatcaattaa ttacaaaaca atgaaaaaag ttcttgcctt
    10441 agttatggct gccggaatgt tcagcatcgt tgcatgcggt ccttctgctg aagaaaaaca
    10501 aaaaatggaa gaaacagcta aagctgctca ggattcaatt gctgctgctg ctcaacaagc
    10561 tgcttctgct gctgaacaag ctgctactga cgctgctcag gttgctaccg atgctgcaca
    10621 aactgtagat tcaactgcag gtgctgtagt aaatcaggct actgaagctg ttaaagaagc
    10681 tgctacaaag taatcagccc ccattttaaa agatataagc tcgctttagc gggcttttct
    10741 tttttatatc aaatcataaa cctttcccgg ataatttttt atcacacctt tcaattctag
    10801 attcaacaac aatgcaggaa tacggtttag cttaacaccc gatcggaaac agattgtatc
    10861 aatatcaacc tttgagtttt ctttcaaaaa atctaccaac tgtttttctt caggatttaa
    10921 gtcttcaaac aaacgtgctt gtttaggaat agatttcaca tctgttttat cccatcccat
    10981 aaaatattca atgtcggcag cagattgaat taaattggct ttattgtctc ttatcaaggc
    11041 attgcagcct tttgaatatt ctttatcaac ctgaccggga atggcaaaaa catctctgtt
    11101 ataagagtta gcaatgtctg cagtaatcaa actaccacct ttaattcccg attcaacaac
    11161 cactacagca tctgccatgc cggcaatgat gcggtttctc tttggaaaat tttctttgtc
    11221 tgcattagtc ttactcataa actcgctgat aacagcacct tctgcctgca ttttttctgc
    11281 agtgttgcga tgttctgccg gatacagtgt atcaagacca tgtgcaacaa ttcctgctgt
    11341 aggtatttta cattttaaac ttgctttgtg tgctgtaatg tctataccaa aagctagtcc
    11401 gctgacaatc atcattccat ctgtttccgc taaatctgca atgagttttt cagtggttgt
    11461 ttttccatag tcgctggctt ttcttgttcc tacaaatgca atacattttt ccggattcag
    11521 attaccattg cctttcacaa aaatcattat tggcgagtca ttacatgctt taagcctttt
    11581 tggatattga tcagaagtga aaaatagcgg ttttatttta tttttctcaa tgaaacgaat
    11641 ttcttcttca gccctcttta gcgactgttc ttttccattt ttaattttat ctgctactat
    11701 ctgaccaaca ttaggaattt ttagtaatgc ctgttgtttt tctttgaaaa tagcctctgc
    11761 accaccacaa tacccgacta ggttttttgc tgtaatgtcg ccaacaccag gtaagagtgt
    11821 cagcgcaagc tgatattgta aaacatcggt tgttaaatta ttttccattg ttgactttca
    11881 agattttcaa gaatagttta ggtttgtgcc ttcacaatct gaatctgcaa agaaaaacat
    11941 gtttagccaa agaataactt tattttattt ttttttcgtt ttaggaattt attctgtgaa
    12001 tgcacagcag caacgtgttt ctggaaaagt taccgatgga gagggtgaat tgcccggtgt
    12061 agctgtttat gccgatgaaa gccacgctac tacatcagat gctaaaggtg agtttgtttt
    12121 aatgcttgat aaagggagtt ggacacttaa agcaaaatta ctcggttacg aaactttcag
    12181 caaagtaatt ctagtaggcg attctaacta tgttcaaatc catttgatac cattaagtaa
    12241 acaattgcaa tcagttgtag tgagtgccgg aaagtttgag cagaaactgg aagaggttac
    12301 agttacgatg gagaccatta aacctaaaag ctttgaaaca acaggcgaca acaatgttga
    12361 aacagcactt gaacatgtaa cgggattgaa tgtaaatgat gggcaagcaa atattcgcgg
    12421 aggcagtgga tttagttatg gtgcaggcag cagggtgcta gtgctggttg atgatatgcc
    12481 catgcttagt gccgatgcca atgatgcaaa atggagtttt ttaccaattg aaaatttaga
    12541 acagatagaa attataaaag gagcatcatc tgcactttat ggttcgtcag ctatgaatgg
    12601 ggtgataaat ttcaggacag gttatgctaa gtcggagccg gtaacaaaaa ttgtacagta
    12661 caatggaatt tatgattcac cacgaagaaa ggagattaag tggtggggaa aagaaactca
    12721 gatgttgagt ggagcaaata tgtttcatgc gcaaaaattt ggacaatggg attttgtagc
    12781 aggtggacat tatctgctcg atgaagggtt tagaaaaggt gaaacagaga agcgagccag
    12841 agttaatata aatacacgat acagatttcc gaagattgaa ggactggcag taggagtaaa
    12901 tgctaattat atggaatcat tcggtggtct tttcattata tggaatgatg ataccacagg
    12961 tgcttatatt ccaagtggag gcgtgaatga ttcaacatct actttgagta attatcacac
    13021 ggttagatac aatattgatc cttttattac ttatcatagt aagaaaggtt ttagtgcaaa
    13081 gtggcgctct cgttatttcc ggactttgaa ttacaatgat accgatcagg aagcttttgc
    13141 agatttatat tattcagaag tgcagttgca acagcgtttt gaaaaatgga atctggtgtt
    13201 aaccggtgga ggtgtgatta ctgatggtgg tgtgcgcagt gatttgtatg gtagtagaaa
    13261 aacatataac aaagcattgt atgcacaggc agataagaaa ttttttgaca ggttgaatct
    13321 ttcattaggc tggcggtggg aacaatttcg tttccgttac agtaaggcag aatccaaacc
    13381 tgtaatcagg gcaggagcaa acctgcgttt attacgtgcc acgtatttac gcagtagcta
    13441 cggacaagga tacagatttc catcaattgc agaaaaatat gtaaaaacaa gagtaggttc
    13501 aattgttatt tatccaaacg attcaatatt ttctgaaagc ggatggagtt ccgagatagg
    13561 gttgaggcag ttatttgctg tcggcaaaca attttcagga tatgtggatg catcggtatt
    13621 cagaagtgag tataagaaca tgctagagtt tgccttcgga agttatggtg atccttttaa
    13681 agatcctctg tttggacttg gatttaaatc actgaatatt ggtaatactc gtattgatgg
    13741 aatggagttt gaacttggtg tgcaggcaac agttcttaaa aaaataaatc ttcagtttgg
    13801 aggaggctac acttatattg atccacgttt gactgatttt gattctttaa aagcatcaac
    13861 aggaaccatt acatacaact tcttaaaata tcgttacaga aatttgttta aaggttatgg
    13921 aagtatagcc tatggaaaag tttctttagc tgtaaattgc agatacaata gctttatgga
    13981 aaacattgat gttgtattta atgaattgct tccaggggtt aaaaggtatc gggccgaaca
    14041 cgataaaggc gactggattt atgatgcaaa catcagttat aaattaaact cccaaatagc
    14101 gttatccgtt atttcaagaa atttgttcaa tcacgaatat tccggaattc cctctcaact
    14161 cgaagcacca agaatgtggt tgtttcaatt tgtaattaca ccttagtgtt ttactatcaa
    14221 tgttaaaatc aaactaaagt tgtgtaaggc atcaactgtt caggatttta tttttaatcg
    14281 tttttgaaac agcttcaccg attgacttaa catgaagttt gtgatatatt tttctgatat
    14341 gagtgtggac tgtttcgtaa ctaactttaa gttctgttgc aatggttttg taggacttac
    14401 cttttactaa taattccagt acttccattt cgcgtttgct aagattgtag tcttcatttt
    14461 tggtttcggt aacttgtgtt ttagcaaaac gagtcagaac ttttcttgca ataaccggtg
    14521 tcattggtgc gcctccggta tagacttcat ggatatggtg aatcagaagc tccatgcttg
    14581 tgtttttcaa aatatagcca ttagcaccag cacatattga atcaaacacc ttatcatcat
    14641 cttcaaagcc ggtgagcatt aaaataggta agccagggaa ctgtttgcga ataattttta
    14701 ctgtttcaat acctgacaat ttaggcatat caatatccat taggataaca tcaggtttta
    14761 aggtgtcaac ttgttttata cattcttcac cggaatcaaa ttctgcaatt aaaagcaatg
    14821 atggtgtgct ttgcagaaga agcgcaacag attctcgcat gtttttattg tcatcaacaa
    14881 taatcactct cagtggctta tcgggaattg ttttattcat gctacaaatt tgaatattct
    14941 tatttgatct tgcaatcacc aattctggtt atattgcatg ttgtttcagg ctaatagtaa
    15001 tttctgtacc cttacccggg gcactgttaa tctggaactc agcatttaaa tcttttgctc
    15061 tggaagccat gttttttaat ccattaccac catttccatt tgtttgcgaa tgattattgg
    15121 ccatatctat tccacaacca ttgtctttta tttttaaaaa tgtcattcca cgatttctgc
    15181 ttaaatggat a
//
LOCUS       DAOOOL010000056        15099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_328_length_15099_cov_114.938115, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000056 DAOOOL010000000
VERSION     DAOOOL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_15099_cov_114.938115"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKU82_06160"
     CDS             complement(<1..336)
                     /locus_tag="PKU82_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOZ82494.1"
                     /translation="MRIRSMANPLTLAELESIAFNRKPVTVDDEIIKSINQSFDFLVR
                     FSKDKIIYGINTGFGPMVQYKVENHELNQLQYNLIRSHSSGTGAVLNEVYARAVVAAR
                     LNNFLQAHSG"
     gene            453..3287
                     /locus_tag="PKU82_06165"
     CDS             453..3287
                     /locus_tag="PKU82_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ82495.1"
                     /translation="MRIFFTAFLLFISGYVSIAQNVISGVIKDGSSGETLIGATIVIK
                     GTTEGAATDIDGKFSFETTRSFPLVLVASFVGFINQEITINNISPLTIKLKKNEVLLK
                     NVTVTGSRISEKQKESPITVEALDYIAIKETPAASFYEGLGQLKGVDLTSASLGFKII
                     NTRGFNSSSPVRSLQLIDGVDNQSPGLNFSLGNFLGSSELDVQKVDLIVGASSAYYGP
                     NAFNGVIAMTTRSPFESPGLQFMVKIGDRKLNEFALRFAQVFKNKKGENKFAYKLNFY
                     RFTALDWKADNYSPTEQSRNTSYNPGGYDAVNVYGDEDINGTDYRNFPGAFPGLMSFN
                     RKGYKEEDLVDYNSNNTKAGLALHYMITPKIEAITSANYGTGTTIYQGDNRYSLKNIK
                     FLQNRVEVREKDKWFIRAYSTSEDAGQSYDAYFTALLLQRSAKSDGHWKNDYENFWNG
                     NYNMSYIRKLPGFPPPPGFGPAYVQWLQSVNPFLLNNYYDSLVHYHQQAQAYASGVGN
                     PQNQNLAFFEPGTARFDSAFNAITSRKSYSEGGSRFYDKSKLYHIQGEYRYPNKWVDG
                     VVGGNYRLYKPNSDGTIFSDTSYVHYNISANNDTLSIDTLRNKLSVYEFGVYAGLEKK
                     ILKDKLKLNATVRFDKNQNFKLLVSPAGSVVYTPNNNHVIRVSFSSAVRNPTLTDQYL
                     FYQVGRATLLGNINGYTNLLTPESVTEFYDSSKSSFKFLNFFNIDPVRPEKVKTIEAG
                     YRATLFNRLYVDLNGYYSWYRDFIGYQIGVDADTFSVNTFGGTIYDLVFNRVVRVASN
                     AQSEVTTQGVSVGLNYYIGKYFALTGNYSWNKLNKKGTDDPLIPAFNTPENKYNIGFN
                     GRDIKGVGFNVNYKWVQGFRYEGSPQFTGEIPSYDMVDAQVNYHLKEAGTTFKLGAMN
                     LFNNKHYEIYGGPKVGRMVYFSILFEVDKL"
     gene            3318..3806
                     /locus_tag="PKU82_06170"
     CDS             3318..3806
                     /locus_tag="PKU82_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOZ82496.1"
                     /translation="MKTSAKSVDEILSNVQEDRKEVFQKLHQVILKNLPKGFEAAISY
                     GGLGYVVPHSIYPDGYHCKPVEPLPFAGIASQKQSINFYHMGMYSDPVLLKWFVSEFP
                     KHSNQKLDMGKSCVRFKKMDDIPYKLIGELMKKMTVKEWIQKYESAYKPKPKEVKTKK
                     GK"
     gene            complement(3877..5001)
                     /locus_tag="PKU82_06175"
     CDS             complement(3877..5001)
                     /locus_tag="PKU82_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82497.1"
                     /translation="MKRILTVAFISLTLGGFAQRAQVQSAYSYLKYEQLDKAKECIDI
                     AVNNENTMNYDKAWYYRGLIYQALYKNEKFANLSNDPLNEALRSYNKALELNPKFEYA
                     DDISEKKKILAQQFADMGYVNYNLKNYAGALTAYEGVVSIMPNDTSSYFNAALCAERA
                     GNAAKAKQYYNKLDEMQYKDAKMYHSYAQLYLSEGDTAKALEVLSRGLSRFPEEKSIL
                     ITQTNIYISSGKTAEALSAINMAIEKDPTNANLYFAKGTLVEKTEKSKDAAIAAYKKA
                     IELKADYFDAYYNLGALYFNDGAHLANEANSIKDNNQYAKAKAVFEAKFKEAKPYLEK
                     AWELNPKDQSTMASLKQLYATINDTVNYAKVKAALDAETK"
     gene            complement(5026..7581)
                     /gene="gyrA"
                     /locus_tag="PKU82_06180"
     CDS             complement(5026..7581)
                     /gene="gyrA"
                     /locus_tag="PKU82_06180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOZ82498.1"
                     /translation="MSEGEKIVRINIEEEMKTAYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLYGMLDLGVLSNRPYKKSARIVGEVLGKYHPHGDASVYDTMVRMAQEWSLRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLKKIAEEMLADIDKDTVDFKLNFDDTLQEPTVLPA
                     KIPNLLVNGAAGIAVGMATNMPPHNLTEVCNAVIAYIDNRDITVEEMLQFVKAPDFPT
                     GGIIYGYSGVKEALLTGRGRVVMRAKANIETLDNGRERIIVTEIPYMINKAEMIKKTA
                     DLVNDKKIEGITDIRDESGRDGMRIVYEIKRDAMASVVLNNLYKHTPLQTSFGVNNIA
                     LVNGRPLLLNLKDLIHYYVEHRHDVLVRKTKFELAEAEKRAHILEGLLIALDHLDEVI
                     ALIRASQTPDEARTGLIEKFALSEIQAKAILDMRLQRLTGLERDKIKEEYAELMKLID
                     YLKKVLGSEELRMLLLKKEVAEILEKYGDERRTEIVYAADDISMEDMIEDEAVVVTIS
                     HMGYIKRTPLTEYRTQNRGGRGSIGSSTRDEDFVEHLFVATNHNYILLFTQKGRCFWL
                     KTYEIPEGNKQFKGRAIQNLISIEPDDKVFAFINVKTLEDEEYINNNYIVMCTRQGVI
                     KKTPLEAYSRVRQNGINAIVINDGDELIEAKLTNGNNEILLAVKSGRAIRFNENTVRT
                     VGRNSIGVRGVSLDHAKDEVIGMVCIKNATEETILVVSEKGYGKRSDLEDYRVTNRGG
                     KGVKTMNITEKTGDLIAIKSVKEDDDLMIINKSGITIRMEVKDLRVMGRNTQGVKLIR
                     LDDDDSIASVAKIDMEITGNGNQKENGNDTEENPTIENGEQNNNQTEENNNPE"
     gene            7886..10450
                     /locus_tag="PKU82_06185"
     CDS             7886..10450
                     /locus_tag="PKU82_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOZ82499.1"
                     /translation="MEAKFSPRVKDVITYSREEALRLSHDYIGTEHLLLGLIREGEGM
                     AIKFLKSLNVNLVELRKSVENAVKGSVNNVSNLNNIPLTKQAEKALKITYLEAKIFKS
                     DIIGTEHLLLSILKDEDNIATQILNQYGINYEIMREELETFKSNFKSEAPQSPSDDPY
                     NKDDDEGPGLGSGSTSKKTGDAKSKTPVLDNFGRDLTKAAEEGKLDPIVGRSKEIERV
                     SQILSRRKKNNPILIGEPGVGKSAIAEGLALRIIQRKVSRVLFNKRIVTLDLASLVAG
                     TKYRGQFEERMKAVMNELEKSPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARGE
                     IQCIGATTLDEYRQYIEKDGALDRRFQKVIIDPTSVDETVEILNNIKDRYEEHHHVNY
                     TPEALKACVTLTNRYITDRFLPDKAIDALDEAGSRVHLSNIHVPENIVELEGKISEIK
                     SEKNRVVRSQKYEEAARLRDSEKQLIDQLEAAKKQWEEETKNQRYAVTDEDVAEVVSM
                     MSGIPLQRVAQAETARLIRMPEELKGKVIGQDEAIKKVVRAIQRNRAGLKDPNKPIGS
                     FIFLGPTGVGKTELAKVLATYLFDTEDALIRIDMSEYMEKFAVSRLIGAPPGYVGYEE
                     GGQLTEKVRRKPYSVVLLDEIEKAHPDVFNLLLQTLDDGQLTDSLGRKVDFKNTIIIM
                     TSNIGSRQLKDFGTGVGFATGAKNAQMDEHARGVIENALKKAFAPEFLNRIDDVVIFN
                     SLSKEDIIKIIDIELEKLYSRIAGLGYKLKLNDEAKDFLADKGNDVNYGARPLKRAIQ
                     KYVEDMLAEEIIKAELQEGDNIEVVFDKEAHLLKANISKSSPPPPKSKKKKEENNS"
     gene            11397..13829
                     /locus_tag="PKU82_06190"
     CDS             11397..13829
                     /locus_tag="PKU82_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82500.1"
                     /translation="MKKPILKLIFLIGLTLHPITSFGQWVTISDTNFVNLLQFYFPTC
                     MNGNQMDTTCNGILTATRLYCPHSSISNLDGIQYFKELDTLECHGNPLTTLPNLPDSL
                     KYLACANGPLASLPPLPNGLVYLYCTMGNLSSLPTLPSTLKHLECWENGITSLPPLPN
                     LIVLNCITNPLGSLPPLPATLLALSCANNSLTSLPSLPLGLVSLDCYANQLTSLPAFP
                     LGLVSLACSANQLTSLPALPSSLKNLFCENNQLSNLPALPALDYLHCNNNLLTSLPTL
                     PSTLYGLRCNNNLLSCLPILPPNIGTTSYYFNISNNPISCLPNYINAMNGTGYLNYAL
                     CAEGDSINNPNGCLSFKGVFGKTYSDTNNPCIWDSTDIPIRNIPIHLLDTNGNLYSTF
                     YTQSNGIYNYILDTSTYKVAIDTTNKLYISNCFSPYVDSIFTLTYIAPVAKDINFNIS
                     CKPQFDVGIKSAYTSGRVFPSLPHKLSVSGGDLSHWYNLNCANGVSGTVTISVTGNLT
                     YDSIPISALTPSNITNNVFTYNILDYGLVNLYSFSIWFTTDTTAQIGDTICVSIDVTP
                     NIGDFDTTNNHYSFCYPVTNSYDPNAKEVYPETVNPLYNGYFVYTIRFQNLGTAAAQN
                     IRILDTLDSNLDPSTFEIINYSHSNISSLKNNVATFRFTNIQLPDSTSNPNASVGFIQ
                     YRIKPLANLPFGTQIKNKAFIYFDFNSGIVTNTVTSNFTSTTDIENINANNTINIYPN
                     PSKGIYNIKFSTSNNLATVIEVYNILSKNISCTTTKNNSCKIDLSAQPNGIYFLRITN
                     CNNSCTRKLVKQ"
     gene            complement(14527..>15099)
                     /locus_tag="PKU82_06195"
     CDS             complement(14527..>15099)
                     /locus_tag="PKU82_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82501.1"
                     /translation="SSAFAQTSYPKGGYKSFEEFQNKQPSIPFVFEITRRTKGDIKMN
                     GGVDYKVTSDSISKSTIKRDIYAISSGDTLYMNCFTHKVQFWYAKVLVEGKYIAFEGG
                     IPMDKNSGVVAAGVAFGPLGGGIAGAHAAMLRYLYIMEAAVGKIKQFDSKYLITLLES
                     FPDLKNQYLNEPKQADEETLLKYLRLINSN"
BASE COUNT         4734 a         2843 c         2724 g         4798 t
ORIGIN      
        1 acctgaatgt gcctgaagaa aattattaag acgtgctgcc acaacagccc ttgcatatac
       61 ttcattgagt actgcacctg tgccactgct gtgactgcgt atcagattat attgtaactg
      121 atttaattcg tgattttcaa ccttgtactg caccataggg ccaaaacccg tattgatgcc
      181 atagatgatt ttatcttttg aaaacctaac taaaaaatca aatgactgat tgattgattt
      241 gatgatttca tcatcaaccg ttacaggttt cctgttaaat gcaattgatt caagctctgc
      301 cagtgtcagc ggattagcca tacttcttat cctcattaat aggatggcaa atgtaatatt
      361 ttcaaaaata tataatgccc gaatcagaca ttgttgtact attgtttatt cggtttcggt
      421 attttacttt taccccaaat aaaaccaagt ttatgaggat attttttaca gcctttttac
      481 tttttatttc cggttatgtt tctattgcac aaaatgtgat atccggtgtt ataaaagacg
      541 gcagcagtgg cgaaacattg ataggtgcta caatcgtcat caaaggaaca acagagggcg
      601 cagctacaga tattgacggc aagttttcgt ttgaaaccac acgtagtttt cctttggttt
      661 tagtggcttc ttttgttggt tttatcaatc aggaaattac aatcaacaat atatcacctc
      721 ttactattaa attgaaaaaa aatgaggtgt tgcttaaaaa tgttaccgta acaggcagcc
      781 gcatttccga gaaacaaaaa gaatcgccaa ttactgttga agcactcgat tatattgcca
      841 ttaaagaaac acctgctgcc agtttttacg aaggcttggg gcagttgaaa ggagttgatt
      901 tgacaagtgc cagtcttgga tttaaaatta ttaatacaag aggatttaac agcagctcgc
      961 cagtgcgttc gttacagtta attgatggtg tagataatca atctccggga ttgaattttt
     1021 cattgggtaa ttttcttggc agcagcgaac ttgatgtgca aaaagttgat ctgattgtag
     1081 gggcaagttc tgcctactat ggccccaatg cttttaatgg tgttatagct atgacaacac
     1141 gcagcccttt tgaatcaccg ggcttgcagt ttatggtaaa aattggtgac agaaaactca
     1201 atgaatttgc attgcgtttt gctcaggttt ttaagaataa gaaaggtgaa aataagtttg
     1261 catataaact caacttttat cgcttcactg ctttagactg gaaagccgat aactattcgc
     1321 caacagaaca atcacgtaat acaagctata acccgggcgg ctatgatgca gtaaatgttt
     1381 atggcgatga agatattaat ggaacagact atagaaattt tccgggtgca tttcccggac
     1441 tgatgtcttt taaccgaaaa ggctacaaag aagaagattt ggttgactac aacagtaaca
     1501 acaccaaggc aggtcttgct ttgcattata tgattacacc aaagatagaa gctattactt
     1561 ctgctaatta tggtacaggt acaacaattt atcagggcga taatcgctac agtctgaaga
     1621 atattaaatt tttacaaaac agagttgagg tcagagaaaa agataaatgg tttataagag
     1681 cttacagcac ttctgaagat gcaggacagt cttatgatgc atattttaca gcactattat
     1741 tacagcgtag tgcaaagagt gatggtcatt ggaaaaatga ctatgagaat ttctggaatg
     1801 gaaattataa catgagttac atcagaaagc tgccaggttt tccgccacct ccaggttttg
     1861 gtcctgctta tgttcaatgg cttcaatctg tgaatccatt cttgttaaac aactattatg
     1921 attcattggt gcattatcat cagcaggcac aggcctatgc cagtggtgtt ggaaatcctc
     1981 aaaatcaaaa cctggcattc ttcgagccgg gaactgcccg tttcgattct gcttttaatg
     2041 ccatcacatc acgtaagtca tattcagaag ggggatcacg tttttatgat aagtccaaac
     2101 tttatcacat acaaggtgaa tatcgttatc caaacaaatg ggttgatggt gtagtaggag
     2161 gaaactatcg tctttacaaa cctaattctg acggaacaat tttttctgat accagttatg
     2221 tgcattataa tatttcagca aacaacgata cattatcaat agatacttta cgcaataaac
     2281 tttctgttta tgaatttggt gtttatgcag gattggaaaa gaaaattctg aaagacaaat
     2341 taaaactcaa cgctacggta cgttttgata aaaatcaaaa ttttaaactg ttagtttctc
     2401 ctgcaggttc tgttgtttat acaccaaata acaatcatgt catcagagtt tcgttttcat
     2461 ctgctgtaag aaatcctaca cttacagatc aatatttatt ttatcaggta ggaagggcta
     2521 ctctgcttgg aaacatcaat ggctacacca accttttaac tcccgaatcg gtaactgagt
     2581 tttatgatag cagcaagtca tcatttaaat ttttgaactt ctttaacatt gatcctgtaa
     2641 gaccggaaaa agttaaaacc attgaagctg gttatcgtgc aacactgttt aaccgtttat
     2701 atgttgattt gaatggatat tattcatggt atcgtgattt tatcggatat cagataggtg
     2761 ttgatgccga cactttttct gtaaacactt ttggtggtac tatttacgat ttggttttta
     2821 atcgtgtagt acgtgtggct tcaaatgcac aaagtgaagt tactacacag ggagtttctg
     2881 tcggattgaa ttattatatc ggaaaatatt ttgcgcttac tggaaattac tcttggaata
     2941 aactgaacaa aaagggcact gatgatccat tgattcctgc cttcaacaca ccggagaata
     3001 aatacaatat tggtttcaac gggcgcgata taaaaggtgt tggttttaat gtgaactaca
     3061 aatgggttca gggttttagg tatgaaggct caccacaatt tacaggcgaa attccttcgt
     3121 acgatatggt tgatgcgcaa gttaattatc atctgaagga ggcaggcaca acttttaagc
     3181 tgggtgcaat gaacttgttc aacaataagc actatgaaat ttatggcgga cccaaagttg
     3241 gcagaatggt ttacttcagt attttgtttg aggtggataa attgtaattt ttgaaactgt
     3301 tttaaaaaag ataaggaatg aaaactagtg caaaaagtgt agatgaaatt ttgtcaaacg
     3361 tacaggaaga taggaaggaa gtttttcaaa aactgcatca ggtaatatta aaaaatttac
     3421 caaaaggttt tgaggcagca atcagttatg gtgggttagg ttatgttgtg ccacattcaa
     3481 tttatcctga tggctatcat tgcaaacctg ttgagccttt gccttttgca ggaatagctt
     3541 ctcaaaagca atctataaat ttttatcaca tgggtatgta ttccgaccct gttttgctaa
     3601 aatggtttgt aagcgaattt ccaaaacaca gcaatcaaaa attggatatg ggaaaaagct
     3661 gtgttcgttt taagaagatg gacgatattc catacaagct cattggtgag ttgatgaaaa
     3721 aaatgacggt taaagagtgg attcaaaaat atgaatcagc ctacaaacct aaacctaagg
     3781 aagtgaaaac gaagaaggga aaataggata atgcaagtaa gagataaaag caaaaaagcc
     3841 ccgcattgcg gggctttttt tatgtgttca aaaattttat tttgtttctg catctaatgc
     3901 tgctttcacc tttgcgtagt ttactgtgtc attgatagtg gcataaagtt gtttcagaga
     3961 agccattgta ctctgatctt taggattcaa ctcccatgct ttttcaagat aaggttttgc
     4021 ttctttaaac tttgcttcaa atactgcctt agcttttgca tattgattat tgtctttgat
     4081 gctattagct tcattggcta aatgcgcacc atcgttaaaa tagagtgcac ccaagttgta
     4141 ataggcatca aaataatctg ctttaagctc aattgctttt ttgtaggcag caatggcggc
     4201 atctttactc ttttcagtct tctctactaa tgtacctttt gcaaaataaa ggttggcatt
     4261 ggtaggatct ttttctattg ccatatttat ggcgctaagt gcttcagcag tttttcctga
     4321 gctgatataa atgttcgtct gtgtaataag aattgatttc tcttcaggga agcgtgataa
     4381 tccacgtgac aatacttcaa gtgcttttgc tgtgtcgcct tcggaaagat acagttgtgc
     4441 atagctgtga tacatttttg catctttata ttgcatctca tctaatttgt tgtagtactg
     4501 ttttgcttta gccgcattgc ctgcacgctc tgcacacaat gctgcattga aatatgatga
     4561 tgtgtcatta ggcatgatag aaactactcc ttcataagct gtcaatgcac ctgcatagtt
     4621 tttcagattg taatttacat aacccatgtc ggcaaattgc tgagctaata tttttttctt
     4681 ttctgaaata tcgtcagcat attcaaattt tgggttcagc tctaaagctt tattgtaaga
     4741 acgtaaagct tcgttaagcg gatcgtttga cagattagcg aatttttcat tcttataaag
     4801 tgcctgataa atcagtccgc gatagtacca tgctttgtca tagttcatgg tgttctcgtt
     4861 gtttacggca atgtcaatac actctttggc tttgtctaac tgttcgtatt taagatagct
     4921 gtaagccgat tgtacctgtg cacgctgtgc aaatccacca agagttaaac tgataaatgc
     4981 aactgttagt attcttttca ttttgctaaa ttgtttgatt aataattatt ctggattatt
     5041 gttttcttct gtttgattat tgttttgttc gccattctca atagtcggat tttcttctgt
     5101 atcgttgccg ttctcctttt gattaccgtt acctgtaatt tccatatcaa tttttgcaac
     5161 cgatgcaatg gagtcatcat catccaatcg aataagcttt acgccctgtg tgtttcgtcc
     5221 cataacgcga agatctttta cctccatacg aattgttatg ccggatttgt tgatgatcat
     5281 cagatcatca tcttctttaa cactctttat ggcaatgagg tcgcctgttt tttcggtaat
     5341 gttcatggtc ttaacacctt taccgccacg gtttgttaca cgataatctt ccaggtcgct
     5401 gcgttttcca taaccttttt cactaactac taagatggtt tcttccgttg cattttttat
     5461 acaaaccatg cctatcactt catctttagc atgatcaaga gaaacacctc ttacaccaat
     5521 agaattacga cccacagtac gaacggtgtt ctcattaaat cgaatagcac gaccggactt
     5581 aacagcaagt aaaatttcat tgttgccatt ggtaagtttt gcttcaatca attcatcacc
     5641 atcatttatc acgatggcat ttataccatt ctgacgtaca cgtgagtagg cctcaagcgg
     5701 tgttttcttt atcactccct gacgggtaca cattacaatg taattgttgt tgatatactc
     5761 ttcgtcttcc aatgtcttaa cattgatgaa agcaaacact ttgtcatcag gttcaatgct
     5821 gattaagttt tggatagcac gtcctttaaa ttgcttgttg ccctcaggaa tttcataagt
     5881 cttcagccag aaacagcgtc ctttttgagt aaacagcaga atgtagttgt gattggttgc
     5941 tacaaataaa tgctctacaa aatcttcatc acgtgtgctg ctgccaattg aaccacgacc
     6001 accacggttt tgtgtgcggt actctgtcag cggtgtgcgt ttgatgtaac ccatgtgaga
     6061 aatggtaaca acaacagctt cgtcttcaat catgtcttcc atgctgatgt cgtctgcagc
     6121 gtaaactatt tctgttctgc gttcatcacc atatttttct aaaatttctg caacttcttt
     6181 tttcaataaa agcatacgca actcttcgct gcccaatact tttttcagat agtcaataag
     6241 tttcatcaat tctgcatact cttctttaat cttgtcgcgc tcaagacctg tcaaacgctg
     6301 taagcgcatg tccaaaatag cttttgcctg aatttcggag agtgcaaatt tttcaatcaa
     6361 tccggtgcgg gcttcatcag gtgtctgcga agcacgaatc aatgcaataa cttcgtctaa
     6421 atgatcgaga gcaatgagta agccttctaa aatgtgagca cgcttttctg cttcggctaa
     6481 ttcaaatttt gttttacgta ccagcacatc atggcgatgt tctacatagt aatggataag
     6541 gtctttgagg ttgagcaaca acggacgacc atttaccaat gcaatattgt ttacgccaaa
     6601 agatgtttgc agaggtgtat gtttgtaaag attgttcagt actacgctgg ccattgcatc
     6661 gcgttttatc tcatacacaa tgcgcatacc atcacgtccg ctttcatcgc ggatgtcagt
     6721 aatgccttca atttttttat cattaacaag gtcggctgtt ttcttaatca tttcagcctt
     6781 gttgatcatg tacggaattt ctgtaacgat aatgcgttcg cgtccattgt caagtgtttc
     6841 aatatttgcc tttgcacgca tcaccacacg acctctgcct gtaagcaaag cttctttaac
     6901 accggaatat ccgtaaataa ttcctcctgt aggaaaatca ggtgctttta caaactgaag
     6961 catttcttca acagtaatat ctctgttgtc aatataggca attactgcat tacacacctc
     7021 tgttaaattg tgtggaggca tgttggttgc catacccaca gcaatacctg cagcaccatt
     7081 aaccaaaaga tttggaattt ttgcaggtaa tacagttggc tcctgtaatg tgtcgtcaaa
     7141 gtttagttta aaatcaacag tatctttatc aatatctgcc aacatctctt ctgcaatttt
     7201 tttcagacgc gcttcagtgt aacgcattgc agcagggctg tcgccatcta tactaccaaa
     7261 gtttccttgt ccgtcaacca aaggatagcg cagactccat tcctgtgcca tacgcaccat
     7321 agtgtcgtaa acagaggcat cgccatgtgg gtggtactta cctaaaacct caccgacaat
     7381 tctggctgat tttttataag gacggttgga taatactcct aaatcgagca ttccgtaaag
     7441 taccctgcgg tgaacgggtt taaagccatc gcgcacatcg ggtaatgcac gtgacacaat
     7501 taccgacatt gaataatcaa tgtaggctgt tttcatttct tcttcaatgt taatgcgaac
     7561 tattttttct ccttctgaca tagtgtgtta atacaatatt tattctgaaa tttatttgaa
     7621 attacgtgtg aaaaagctta aattggcaag ggtaaacatg tcatattaaa atacctcttt
     7681 ttgcttgttt tgtcatgtct gcgaaggtaa tgttttattg gcatatttta tgatgaaaac
     7741 cccttaaaat gttatgaaga tttactaaca atgcaaaaac aaaaaatatt gttaagtttg
     7801 ttgttcacaa ttaataaaat tgcatcagaa gtaaaagaaa actatttcgg atttttaccg
     7861 ttaaaattga ataaatacac agaatatgga agcaaaattt agccccagag taaaggatgt
     7921 gataacctac agtagagaag aagccctacg attaagtcat gattatatcg gcacagagca
     7981 tttgttgctg ggtcttatcc gtgaaggcga aggcatggcc ataaagtttt taaaatcgct
     8041 caatgtaaac ttggttgaat tacgcaagtc agttgagaat gccgttaaag gaagtgttaa
     8101 taacgtcagc aaccttaata atattccact tactaagcag gcagaaaaag ccttaaaaat
     8161 tacctatctt gaagctaaaa tatttaaaag cgatattatc ggcacagaac atttgttgct
     8221 ttcaattcta aaggacgaag acaatattgc tacacaaata ttaaatcagt atggtatcaa
     8281 ctatgaaatt atgcgcgaag agctagaaac cttcaagtct aatttcaagt cagaagcacc
     8341 acaatcgcca tctgatgatc cctataacaa ggacgatgat gaaggtccgg ggttgggcag
     8401 tggttccaca tcgaaaaaaa ccggtgatgc caaatcaaaa actcccgtac tcgataactt
     8461 tggtcgcgat ttgactaagg cagccgaaga aggaaagtta gaccctattg taggacgaag
     8521 caaagagatt gaaagagttt cgcaaattct gtcacgcaga aagaaaaata atccgattct
     8581 tattggtgaa cccggtgtag gaaaatctgc cattgccgag ggtcttgcct tgcgcatcat
     8641 tcaacgcaag gtttccagag tgcttttcaa taagagaatt gtaacacttg atttagcatc
     8701 attagttgca ggaacaaaat atcgcggtca gtttgaagag cggatgaaag cagtgatgaa
     8761 tgagttggaa aaatcgcccg atgtaatttt gtttattgat gaaatacata ccatcattgg
     8821 tgccggtggt gccagcggtt cgcttgatgc ttccaacatg tttaaacctg cattggctcg
     8881 tggcgaaata caatgtatcg gtgcaacaac attagatgaa taccgtcagt atattgaaaa
     8941 agacggggcc ttagacagac gttttcagaa agttattatt gatcctactt ctgttgacga
     9001 gacagtagaa attctcaata atattaaaga ccgttatgag gagcatcacc atgtaaatta
     9061 tactccggag gcattgaaag catgtgtaac gcttactaac agatacatta ccgatcgttt
     9121 tctgcctgac aaggcaatag atgcattaga cgaagcaggt tcacgagtgc atctttcaaa
     9181 cattcatgtg cctgaaaata ttgttgagtt ggaaggaaaa atttcagaga taaaatcaga
     9241 gaagaacagg gttgtgcgca gtcagaaata cgaagaggca gcacgcctgc gcgattccga
     9301 aaaacaattg atagaccaac ttgaagcggc taaaaagcaa tgggaagagg agacaaaaaa
     9361 tcaacgctat gccgtaacag atgaagatgt tgcagaagta gtttccatga tgagtggtat
     9421 tccattgcag cgtgtggcac aggcagaaac agcacgactc attcgtatgc cggaagaatt
     9481 aaaaggtaaa gtgattggtc aggatgaagc aattaaaaaa gttgttcgtg ccattcagcg
     9541 taatcgtgca ggattaaaag accctaacaa gcctatcggc tcattcattt tcttaggccc
     9601 tacaggtgtg ggtaaaacag aattggccaa agtacttgcc acttatttat tcgatactga
     9661 agatgcatta atccgtattg atatgagcga atacatggag aaatttgctg tatcgcgact
     9721 cattggtgcg cctcccggat atgttggata tgaagaaggc gggcagctta cagagaaggt
     9781 aagacgcaag ccatattctg ttgttttgct cgatgaaatt gagaaagcac atccggatgt
     9841 gttcaactta ttattacaaa cattggatga tggacagctt accgactcat taggtcgtaa
     9901 ggttgatttt aaaaacacca tcatcatcat gacaagtaac attggctcgc gtcagttaaa
     9961 agattttggt acgggagtag gttttgctac gggtgccaag aatgcacaaa tggacgagca
    10021 tgccagaggt gttatagaaa atgctttaaa gaaagctttt gcacctgaat tccttaatcg
    10081 tattgatgat gtagtaatat ttaattcgct cagcaaagaa gatattatta aaattattga
    10141 tattgaattg gagaaacttt attcgcgcat tgcaggctta ggttataaac ttaaacttaa
    10201 tgatgaggct aaagattttc ttgcagataa aggaaatgat gtaaactatg gtgcacgtcc
    10261 gctcaagcga gcaattcaaa aatatgttga agacatgctt gcagaggaaa tcataaaagc
    10321 agaattgcag gaaggtgata acatagaagt ggtttttgac aaggaagctc atttgctaaa
    10381 ggcaaatatt tccaagtcgt caccaccacc accaaagtca aaaaagaaaa aagaagagaa
    10441 taattcttaa aaacaaaaag gctgctctat tgagtagcct tttttgcttg ggttacatac
    10501 ttgtaatatt taaagctatc gctttcatgt ttttttctgt acttatatac tttcaaagtc
    10561 tcccacattt aaagggaatt tgaaataata gaagcattga agggatgata cgttttttct
    10621 gaaaatagaa aacatttaat tacaccggac aggttgctaa ggtcttttca gagttattta
    10681 aacaacattt tattttcttc atccacattt ttcatcagtg acattttaga aaatttcaac
    10741 cttctaaact tttaaaactt tacaactttc aaactctaac catacgacct cttgaagaat
    10801 tgcgtaaagc aaaagtcgtg agggaaagta aaaagaaaac aagctgtccg aagttacagc
    10861 gtgcagcgct gtaataagtt tttgttttcg tgggcaagca gacttttgta gcttttttca
    10921 agcagtcttg attttttgat tcttttgtat caagacaaaa gaatggaaaa gaataaaaag
    10981 aaaacagcaa tttaatttca cctgtcattt cgcaccgccc ctgacagtgt gatttaacac
    11041 aaacaattaa ttttaatata gcctaattaa ctgctattgc cttcttaaat catttttctt
    11101 tttacttttg agctaatgaa tagttttaat tccgaaagtt atcggaaaga ctgacgttat
    11161 acgcaatccg tgtcgctccg atgaaacatt ctgcaaactt gaaatagtat cactccaaaa
    11221 gccggcaaag ccgttttggc tatcccttct gtaaaataaa aagagaagta cgtcattttt
    11281 tattttttta tttggcgtac ttcacatttt attttacccc accgcacaaa taccgttttc
    11341 aatctatata tttgtttcca aattaacttt agtacttttg acaaacaata tatgtaatga
    11401 aaaaaccaat actgaaactt atttttctta tcggattaac attacatcct ataaccagtt
    11461 ttgggcagtg ggtaacaata tccgatacca attttgtaaa cctacttcaa ttttattttc
    11521 caacctgcat gaatggaaat caaatggaca ccacatgcaa tggcattctt actgcaacta
    11581 gattgtattg tcctcatagt tcaattagca atttagacgg cattcaatat ttcaaagaat
    11641 tagatacttt ggaatgtcac ggtaatccac ttactacttt accaaatctc cctgactcct
    11701 taaaatactt agcgtgtgct aatggcccac ttgcatcact tccaccttta cctaatggct
    11761 tagtttattt atattgtaca atggggaatc tttcttcttt accaactcta ccttcaacat
    11821 taaaacattt agaatgctgg gaaaatggaa taacaagcct tcccccactt cctaacttaa
    11881 tagttttaaa ttgtattact aatcccctcg gttcattacc accattaccc gccaccttgt
    11941 tggctttatc ctgtgcaaat aattcattaa cttctcttcc atcgcttcca ttaggattag
    12001 tctcactgga ctgttatgca aaccaattaa caagtttacc tgcctttcca ttaggattag
    12061 tctcactggc ctgttctgca aaccaattaa caagtttacc tgccttacct tcctcattaa
    12121 aaaatttatt ttgcgagaat aatcaactaa gcaatctccc agcactgcca gcattggatt
    12181 atttacattg taataataac ttacttacaa gtctgccaac acttccaagt acattgtatg
    12241 gattaaggtg taacaacaat ctgctatcat gtcttccaat tttacccccc aatattggta
    12301 ctacaagtta ttacttcaac atatccaata acccgatttc ctgtttgccg aattacataa
    12361 atgcgatgaa cggaacaggc tatttaaatt atgcgctgtg tgctgaagga gattctatta
    12421 ataatccgaa tggatgctta agttttaaag gtgtgttcgg aaagacttat tctgatacaa
    12481 acaatccttg catttgggat agcaccgata ttcctattag aaatatacct attcatttac
    12541 ttgatacaaa tggaaatttg tattccacat tttatacaca aagcaatggg atttacaatt
    12601 atattttaga tacatcaacc tataaagttg caattgatac aacgaataaa ctttatattt
    12661 ccaattgctt ttctccttat gtagattcta ttttcacact aacatacatt gctccagttg
    12721 ccaaagacat aaattttaat atcagttgca aaccacagtt tgatgtagga ataaaaagcg
    12781 catatacgag cggaagagtt tttccttcac taccacacaa gttatcagtt tcgggaggag
    12841 atttaagcca ttggtacaat ttaaattgcg ccaatggagt aagcggcact gtaacaatta
    12901 gtgtcacagg taatttgact tatgacagca ttccgattag cgcattaaca ccatctaaca
    12961 ttactaataa cgtatttaca tataacatcc ttgattacgg gttagtaaat ctctattcat
    13021 tttctatttg gtttactacc gatacaactg cacaaattgg cgataccatt tgcgtttcaa
    13081 tagatgttac accaaacatt ggagattttg atactaccaa taaccattat tctttttgtt
    13141 atccagttac taattcctac gacccaaacg ccaaagaagt atatccggaa acagtaaatc
    13201 cgctttacaa tggttacttt gtttatacaa ttcgttttca aaacttagga acagcagcag
    13261 cacaaaacat tagaatactt gacactttag atagcaactt agacccaagc acatttgaaa
    13321 ttattaatta cagtcactcc aacataagtt cgttaaaaaa caatgtggct acatttcgct
    13381 tcacaaacat tcaattgccc gacagcacaa gcaacccgaa tgcaagtgtt ggttttattc
    13441 agtataggat taaacctctt gcaaacctgc cgtttggtac acaaataaaa aataaagcat
    13501 ttatttattt tgatttcaat tcaggcatag ttacaaatac agttacaagt aatttcacga
    13561 gtaccacaga cattgaaaac attaatgcta acaacacaat aaatatatat ccaaatccaa
    13621 gcaaaggaat ttacaacata aaattttcaa cttcaaataa tttagcaaca gttattgaag
    13681 tgtacaatat tttgtctaaa aatatttcct gtactactac aaaaaataat tcatgcaaaa
    13741 ttgatttatc agctcaacca aatggtattt attttttaag aattactaat tgcaacaatt
    13801 catgtacgcg aaaacttgta aagcagtaaa tgtttcttat gactatattc caatcgccat
    13861 catatttgct ttcacaatta aaagccgcgc agccccaccc caaacccaaa gcttttaata
    13921 gagaaagcaa gccttgtagg cgcgagcgtc attcacgttc gtgccattaa accttatagg
    13981 cgcaagcgtc atttccgctc ataactttta agacattatt gaaagactat aaaataattt
    14041 gccaaagtat tttttgaatc gcctgccggc aaggcaggga aagaagtagt gaaagcgtaa
    14101 aataaaatac tcaactggaa aacccgacac tcaatgacac agaaactcaa tacggacaat
    14161 ggtttgcaaa gaaagacgag aagaaccact ggctataata agggataaga ccatcagagc
    14221 aattccgaaa atatagactg aaatttcaat taagagtaat ttttacactc gaatacaaaa
    14281 caagagtaat tttttattat ttttacactc gtaaaatcaa taaaagtaaa atttacccct
    14341 ttgtagccgc gagcgtcctt tccgctcgtg ccctttaact cttacgactt ccagtcgcat
    14401 tcaaagttcg ctaagtgtat ttgtcaaacc ggttatgcat ctaaaataga ataaagcctt
    14461 gatttgactt acaggggcta tgcgccttgt caggttaaat tattactgtt aacggcagta
    14521 aagttgtcaa ttagaattaa tcagtctaag atatttcaac aaagtttctt catctgcctg
    14581 ttttggctca tttaagtatt gattttttaa atcaggaaaa gactcaagca gagtaattaa
    14641 atatttactg tcaaactgtt taatttttcc aacagcagct tccattatat ataaatatcg
    14701 cagcattgct gcatgcgcac cggcaatccc tcctcccaaa ggcccaaatg caacacctgc
    14761 tgcaacaaca ccactatttt tgtccattgg aatgccgcct tcaaatgcaa tatattttcc
    14821 ttctactaaa acttttgcat accaaaattg aactttgtgc gtaaaacaat tcatgtataa
    14881 tgtgtcacca cttgaaattg cataaatatc cctcttgatg gtggatttac taatcgaatc
    14941 acttgtaact ttataatcaa caccaccatt cattttaatg tcaccttttg ttcttctggt
    15001 tatttcaaat acaaatggaa tagaaggttg cttattttga aattcttcaa aggatttgta
    15061 tccgccttta ggatagcttg tttgagcaaa agcactgct
//
LOCUS       DAOOOL010000057        15001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_333_length_15001_cov_86.768634, whole genome shotgun sequence.
ACCESSION   DAOOOL010000057 DAOOOL010000000
VERSION     DAOOOL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15001
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_15001_cov_86.768634"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKU82_06200"
     CDS             <1..379
                     /locus_tag="PKU82_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82502.1"
                     /translation="TTIEDKRPVFYFYLSIDGKDINEVKESTYDGVASPKEFVLVKAT
                     TSKSGRQIQIARKSAFSQEEGFAKGVVDFKIEKVSNRLYKVYFENDVPAGEYAFYYNK
                     SSDQKKSLKLYDFSLRNNITVVK"
     gene            392..985
                     /locus_tag="PKU82_06205"
     CDS             392..985
                     /locus_tag="PKU82_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82503.1"
                     /translation="MKSFNIFKLSAFAFLCFTIVSCSQQDSGKQSENNNVQESEVASQ
                     LFSGLKSKLNSDDKKQISTLTGLTYDAASKTFMTEGEGVEVQVVPVDINNDKTEDIFV
                     ITYSSFLYGNTGQGFSLFMKDESGKFKNTLSLPGIPELLYESSESMPSIRVGGPGFDF
                     PIYKWKSHSYKLVKNSGKVSKALSLEEAGQQYTNSIK"
     gene            1043..1318
                     /locus_tag="PKU82_06210"
     CDS             1043..1318
                     /locus_tag="PKU82_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82504.1"
                     /translation="MLFTLGSCKKSTVDADLDYMNNLVTQLENKSKTEGLKFDDYMVL
                     QNDLLQMQHESEILTDRSKFTPEQKKRAEEITNRLGTLILKNKNIQL"
     gene            1495..2265
                     /locus_tag="PKU82_06215"
     CDS             1495..2265
                     /locus_tag="PKU82_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOZ82505.1"
                     /translation="MKKKTKLMLAILICPIAIHAQIRLGQFKPSYNAEVFLGGSASFS
                     YGKFINEHKEFHEVSFSDADISGKIKPIVFFDAGGQFRVVLGEEDLLSFFSVSMGAFY
                     SKRGFTHLYSYSSDNSVQNINDEMSYTEKYRINQISIPILIRYGKKWFVEIGPSFDHF
                     ISATRKQILKREISGSGAYDGGFSTTETNQDNLNKNMFNEHKAGFILGLGTTLGDRFS
                     VRLTNHIFAKAYDKGSNYKSYNLQLEIMYNLFKLNNNE"
     gene            2258..3079
                     /locus_tag="PKU82_06220"
     CDS             2258..3079
                     /locus_tag="PKU82_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82506.1"
                     /translation="MNNRLNKILIAAIPVLLIIGSCKKESTDIDYGVNEYQKDMINKA
                     MVVGSGGKLYEGPMPTATGGTIAITDKPNAVQVSAGVLLYFNYNTSNNANLCTFYVQI
                     DSADSYWAAPIEFDTIAQRPYVRILIPNFVRNGDYKLKFAVGDCAGNVSTIANTDVSV
                     TDPLGCGASFSGNVGITALVANLGSKAGTATISYDMYSIPDRLDIRYDGQWIASTGTL
                     LSPSGYPNCAISPDGFVSGSHSVTFNYDPGKSRLVEIYVSGCNSGTAWQIEVVCP"
     gene            3295..5949
                     /gene="cphA"
                     /locus_tag="PKU82_06225"
     CDS             3295..5949
                     /gene="cphA"
                     /locus_tag="PKU82_06225"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOZ82507.1"
                     /translation="MRILEIRPMRGPNFWSIRRHKLIVMKLDLQEMEERPTDKIDGFL
                     ERLKSMFPSMYSHRCSEDHEGGFFKRVEEGTWMGHVIEHIALELQTLAGMDTGFGRTR
                     GTGEHGVYHVVFSYIEEKAGVYAARASVRIAQALIDGTGYDLQNDIQQLREIREDERL
                     GPSTGSIVEEAASRGIPWIRLNRHSLVQLGYGINQRRIQATIASTTSSIAVEIACDKE
                     DTKNLLEAQSIPVPRGKIIRDEDGLKDAVEDLGYPLVIKPLNGNHGRGATINIVNWDQ
                     AVEALEIAKKISRSVIVEKYITGYDHRILVINYKFICAAQRKPAAVVGDGKSTIQQLV
                     DAVNADPRRGYGHENVLTSIKIDEMTQNILEEKELTLQSVLKKGEELFLKRTANLSTG
                     GTATDVTDIVHPYNVFMCERIARIIGLDICGIDIMTPDISEPLTETGGAVLEVNAAPG
                     FRMHIAPTDGLPRNVAEPVVDMLYPPGSNYRIPIIAVTGTNGKTTTTRLIAHIAKTCG
                     HKVGFTTTDGIYIQNQMLQRGDCTGPQSAEFVLKDPTVDFAVLETARGGILRAGLGFH
                     RCDIAVVTNVAADHLGLKGINTLEEMSKVKAVVPESVQPNGYAILNADDELVSNMARN
                     LDCKVAYFSLDENNPLIKRHCEDGGLAAILENGFVTICKGTWKLRVHKVINIPLTFSG
                     KAVFMIQNILPAILSAFIRNFKIEDIRLALETFIPSPVQTPGRMNMFQFKKFNVMVDY
                     AHNPAGFQAIARFLEKVDAKPKVGVIAGVGDRRDEDIIALGTLAAKMFDDIIIRQDRN
                     LRGRTEQEIIDFMLDGIKAVDPTKPVKVIPLEKEAIDYAIKNGKKGSFIVICSDVIPD
                     ALDQIMKFKEKEDQFEMHPDDIPNQSNR"
     gene            6044..8065
                     /locus_tag="PKU82_06230"
     CDS             6044..8065
                     /locus_tag="PKU82_06230"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ82508.1"
                     /translation="MSRFLSRIIFCVCLFFSHCVLYASDTTPTAQQKQIDTRISEAEE
                     IYKRDFKHAYVITLEAKSHAVKINYLQGIVASSCLQSIIESQTNKSIPAIAALTNLLQ
                     SSNGINLKLKQQLLSTLGICYFYIGKLDSAQFYFTESISSYSAHDTSIQLFNSCLQLS
                     EVFIKEGIPSKALDLYGKAYKIYQQNTSKEGEAWLNLVLGKIYYRQNLYEQSISTINT
                     TISQFASLNSLTGQISSLIQNGNSYYKQMLDDSAANCYQQALMYATTLGDERNKSICY
                     SNLSRIYLEHNKTKQALETALHALEVIHPGNYPEIEAGTYQQLGDIYVELGQKSKAIE
                     WIQKALLLAKDNNNKTIERDCYKSLSELYASIKQYEPAMENLLQAYRIKDSIQPIAFN
                     NRLAEMEARYSLEKKESTIKLLNQQSLINELRIKEQKENIRKQNALVIILFLLIATII
                     IALYFYLRWRKQAELINQQNTIRQTEENERMRIAKDIHDELGSGLTKIKFLSEILEMK
                     TKDQEQLKETLQTITGTSHQLIDNMRDMIWNMNPMNSTLDNLIARVREFSTEYLEDFP
                     IEVKYDLPENIPSEKISIEISRNILMIVKESLQNIVKHSNASKVSIRIEIQPVFKLII
                     TENGIGFNLNEKTTGNGLRNITERSKAIGASLKISSKPGEGSILSLEKI"
     gene            8119..8742
                     /locus_tag="PKU82_06235"
     CDS             8119..8742
                     /locus_tag="PKU82_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82509.1"
                     /translation="MTIQVGIIEDEKHIRENLQILIDGSEGFSCKHLFANAEDAINII
                     PTLQLDVVLTDIQLGGKTGIDCILELKDKCPSTNFLICTSFDDSEHVFKALKTGAMGY
                     LTKTTQPSKLLDSIVDVYRGGSPMNSHIARKVVSSFMETPVSPNYEKLSERERLIVDL
                     LSKGMRYKEIADQLFLSTETVRTHIRNIYHKLQVNSRTEALNKVFKK"
     gene            8810..10432
                     /locus_tag="PKU82_06240"
     CDS             8810..10432
                     /locus_tag="PKU82_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82510.1"
                     /translation="MKQMLPKVTAVWLASAILSPTLLIAQGNETTHELSKPAQKGILA
                     NAELSADGNIRMTYKMKVDKKSDQVNYEDYVFDKNLKFIGVEKTKEDKQTQPDQKVTL
                     IKAFVGGTNSFNVMSMSLNLQQEVWERNWNFDKQKYQWGSRISKEVVKPKNNDSKYRG
                     FAAYGNDDDGSVLILASYDSGEDDQFVCLHVDKGLNMTETKLPLQGKYSLVYCGTLKS
                     GNIFAVFAPNEGMSDTKKYYYIECSADSKVAKSSEFTSPSPNMLIMDYSEANGDLYFI
                     AGSDKSTEAYNKVFTSYAPIDNPGYSTSNNKQMDKYEKRAMGTEFANLHLLRFSDGKL
                     AFASTEPVKDFKTKMVTPPSQKKGQPYTGKKIVIENLFVAPSGDVLVAGQLSDKKVVN
                     GGNSYEFRYYDYVCVQFDNQGKLVAQYAVNKMLDDSKNEVLSAIQNFVPSADGKSVYW
                     EILEVQAAKYYASVAAAYSGNYSLSSHYFPRIANITLSNHSVSNFTELGEKGKYLVYH
                     SITSLTDKDNNRFYIGHDEDYKNIWLGKYSFR"
     gene            10466..11188
                     /locus_tag="PKU82_06245"
     CDS             10466..11188
                     /locus_tag="PKU82_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82511.1"
                     /translation="MKKLIIPILCIAILAFTSCKKKDKDNPHYTCSSCVTQAEALAAN
                     DGSSKGVYKGIIIGSSGTIKFSIQNGTNDITATMVVDGVSATLTSTVSWVSGQPYIAP
                     FTGTLNGQAVSITFSVQNNGSTPQIISSNIPGHPNAVFTIIKETSNALVEGFEGTYST
                     TEPESGTFNILLSRPLGQWGGISRENGQTSTNDVSGTITSSGDLYDDNNIKVGTLSGD
                     EIDGTFTDNSGNTVTVDGQRTL"
     gene            complement(11418..12776)
                     /locus_tag="PKU82_06250"
     CDS             complement(11418..12776)
                     /locus_tag="PKU82_06250"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOZ82512.1"
                     /translation="MNGIAHSNFAGNMGIEMNPASIATMPYRFEFNILSGDIFFQNDY
                     IYFPASDIPTGKFARFAPLRHEDYSDHYDKTPKNAYGNLLLKGPSAVWRNEEAAFGIH
                     FSLRSQLSAIKVPYHLAKFLWEGSDYSPQHKIPWNSTAFSAGALVFAEAGFTFAKTIL
                     TNRQNITTAGGTVNFLLGMSGTYINSTVLDYYFTDASLLNIKNIEVDYGHSIPNDDKN
                     NYLHPRGAGASMSFGVQHFFNYISQAYKPGMSGIKLKKYDLKLGLSLIDLGFIRFNGN
                     ARTFNIRNASAVWPGFDTTKITGYEHADSIFSAKLYGDANLSRSGSGFNMILPAAASF
                     QADWSITPKYYLNLAVIQNIQIFDRSVTRASQVSLTPRFETRRVEVSLPLSLYQYNFF
                     RAGIAVRYKWFVLGSDNLGWWPGLYEMNGLDFYFGFKYTSDIYDNIRFKKGKGGHCPA
                     YK"
     gene            12994..13452
                     /locus_tag="PKU82_06255"
     CDS             12994..13452
                     /locus_tag="PKU82_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82513.1"
                     /translation="MKKFYFLILSIFVVTSSCKEKNKLSDLDWIIGKWEGTARNGNIF
                     YEEWKKQSDTAFINNNYHFEGEKQVFGGTSKIVLRNNHLYYINDSDSLKILQWEAEGH
                     NSTSMTFYNEKITPYNRITFSLSEQNTWDAKLMGKKDTVKYSLKRKIMPY"
     gene            13550..>15001
                     /locus_tag="PKU82_06260"
     CDS             13550..>15001
                     /locus_tag="PKU82_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82514.1"
                     /translation="MKKIFLLLFICILTYSAGYSQSCFTINSILVDACGNPEGENEMV
                     RFTVGASPLQVSSLNVSWPSTNLNFLGICQNANTAQKVADLNATIQSCGYLLEPSNDI
                     LPQNSKVILVTSENFNTTFNSFANLTDTMYVIFQCAGNTQGHFRNYSATVTTPRILTM
                     NFGAVCSQTVQYFPNQLVNQFGQLDTAAQDGSSVGFDSLGNPTYYNNGCQAPIISLTA
                     TLQSSATTVCSGNSVTLTASNLTGNYSGYFWSGGNGTLSTPNNLVTTYQTNATFTGTD
                     TIQFSMLGQCNDTITSNIYLIVQNGQPASITGPSTVCTGDSITLTASSGTAYQWSNGA
                     TTQSITVLTPGTYTVTVTGTCGTPTATATITGGTAPNITVTPGTNVSICAGQSVTLAA
                     SGASSYQWSTGATTSSISVNTAGTYTVSSTNACGTSSVTINVTQTTTPVVNITSNSGT
                     EICPGQSLTLTASGASTYVWSGGQTTPSITINTA"
BASE COUNT         5145 a         2767 c         2739 g         4350 t
ORIGIN      
        1 tactacaatt gaagataaac gtcctgtatt ctatttctat ctttctattg acggaaaaga
       61 cattaacgaa gttaaagaaa gtacctatga tggagtagcg agccctaaag aatttgtctt
      121 ggtaaaagca accacttcta aaagcggtcg tcagatacag atagctagga aatcagcctt
      181 ttcacaagaa gaaggttttg ccaaaggtgt agttgacttt aaaatcgaaa aagtatcaaa
      241 cagactttat aaagtttact ttgaaaatga tgttcctgca ggtgagtatg ctttttatta
      301 taacaaaagc agtgatcaga aaaaatcgct taagctttat gacttctcat tacggaataa
      361 tattacagtt gttaaataaa acttattagc aatgaaaagc ttcaatattt tcaaactttc
      421 tgccttcgca tttttatgct tcacaatcgt cagttgctca cagcaagact caggcaaaca
      481 atctgaaaac aataatgtac aggagtcaga agttgcaagt caactattct ccggtctgaa
      541 atcaaaactt aatagtgatg ataaaaaaca aatcagtaca ttaacaggat tgacctatga
      601 tgcagcaagt aaaactttta tgactgaagg cgaaggtgta gaggttcagg tagtgccggt
      661 tgacatcaac aatgacaaaa ccgaagatat atttgtaatc acctacagtt cgttcttata
      721 tggcaataca gggcagggtt ttagtttgtt tatgaaagat gagagtggta aattcaagaa
      781 tacactttca ctgcccggta ttccagaact tctctatgaa tcatctgaaa gtatgccttc
      841 aattcgtgta ggaggccccg gatttgactt cccaatttac aaatggaaaa gtcattctta
      901 taaattagta aaaaactcag gcaaagttag caaggcttta agtcttgaag aggcaggaca
      961 acaatacacg aactcaataa aataatccat aactaaaatg gcctgtagtg ttcgatacca
     1021 aattatatgt cttgcatttt caatgctatt tacgttaggg tcgtgcaaga aaagtacagt
     1081 agatgccgac ttagactaca tgaataattt agttacacaa ttagagaata agagtaaaac
     1141 agaaggattg aaatttgatg actacatggt gcttcagaat gatttacttc aaatgcagca
     1201 tgaatctgaa attctgactg acaggtcgaa atttactccg gaacaaaaaa agcgtgcaga
     1261 agaaattaca aatcgcttag gaacactgat attaaaaaac aaaaacatac aattataaaa
     1321 ccaattaaaa tgaaaaaaca aattacacaa ttttttatcg ctgttatagc agtgatggct
     1381 acaggctttg ttaaaggtcg taattctaaa aataattaat cagatcaata gaaacacttg
     1441 gatactaact tttaatttct atattgcact tctaaacaaa aaaatcatta aagtatgaag
     1501 aaaaaaacca aactcatgct agctatttta atctgtccga tagctatcca tgcacaaatc
     1561 agattagggc agtttaagcc aagttacaac gcagaggtct ttttaggtgg aagcgcctca
     1621 tttagctatg gcaagtttat taacgagcac aaagagtttc atgaagtaag cttctctgac
     1681 gctgacattt ccgggaaaat taaaccaata gttttctttg atgccggggg tcaatttaga
     1741 gttgttttgg gtgaagaaga tttactctcg tttttcagtg tttcaatggg tgcattttat
     1801 tcaaagaggg gatttactca cttatattcc tactcctctg ataattcagt acaaaacatt
     1861 aatgatgaga tgagctatac agagaagtac agaatcaacc aaatttctat tccaatttta
     1921 atacgttatg ggaaaaaatg gtttgtagag atcggcccat ctttcgacca ttttatctct
     1981 gcaacgcgta aacaaatatt aaaacgagaa atcagtggtt caggagccta tgatggtggc
     2041 ttctctacta cagaaaccaa tcaggataat ttaaataaga atatgttcaa cgagcataaa
     2101 gcaggtttca ttctcggatt gggcaccaca ctaggcgatc gtttttcagt gcgacttacc
     2161 aatcacattt ttgcaaaagc ttacgacaaa ggaagtaact ataaaagtta taatcttcaa
     2221 cttgaaatta tgtataacct atttaaatta aacaacaatg aataatagac tgaataaaat
     2281 attaattgca gcaataccgg tattattaat aattgggtcc tgtaaaaaag aatctaccga
     2341 tattgattat ggtgtcaatg agtatcaaaa agacatgatc aacaaagcca tggtagttgg
     2401 cagtggcgga aaactttatg aaggacctat gccgactgct acaggaggta ccattgctat
     2461 tacagacaaa ccaaatgctg ttcaggtaag tgctggtgtt ttgctatatt tcaattataa
     2521 cacaagcaat aatgctaatc tctgtacttt ttatgttcaa attgacagtg cagattccta
     2581 ttgggccgca ccaattgaat tcgacacaat tgctcaacgg ccttatgtac gtatccttat
     2641 tcccaacttc gtgagaaatg gggattacaa attaaaattt gccgttggtg attgtgcggg
     2701 taatgtcagt acaatagcca atactgatgt ttctgttacc gacccattag gatgtggcgc
     2761 cagtttctca ggtaatgtag gaataacagc attggttgcc aatcttggca gcaaagccgg
     2821 aacagcaaca atttcatacg acatgtatag catccctgac agactcgaca ttcgttatga
     2881 tggtcagtgg atagcttcaa caggcacact cctatcacct tcaggatacc ctaactgtgc
     2941 aatatctcct gatggttttg tttctggttc tcattcagta acttttaatt atgaccctgg
     3001 aaaaagcaga ttagttgaaa tttatgttag cggttgtaat agtggtactg catggcaaat
     3061 agaagttgtt tgtccttaat tagcgtaaaa taatccttaa aaagaggcta tttcaaatat
     3121 tgagataacc tcttttttct tagatgcata tctgaatcaa aaattgaata atacttcgat
     3181 tcactaaatc tattctgtat tgaattaagc tatttttctc ttcgcagttt caccaagttt
     3241 ttcaatattc aaatcaagtc tctatattcg tgccacaacc aaaaggtttt ttttatgagg
     3301 atacttgaaa ttcgcccaat gagagggccc aacttctgga gcatacgcag acataaactt
     3361 attgtaatga aactcgacct tcaggagatg gaagagcgac ctactgacaa aattgatggc
     3421 tttttagaaa gattaaaaag catgtttcca agtatgtatt cgcaccgatg cagcgaagac
     3481 catgaaggag gattttttaa acgtgttgaa gaaggcacct ggatgggaca tgttattgag
     3541 cacattgccc tcgaactaca aacacttgca ggcatggaca caggctttgg ccgtacgcgc
     3601 ggcacaggcg aacatggtgt atatcacgtt gtgttctcct acattgaaga aaaagcaggt
     3661 gtgtatgcag cacgtgcctc tgtgcgtatt gcacaggcat taattgatgg cacaggctat
     3721 gatttgcaaa atgacattca gcaattgcgt gagatacgtg aagatgaacg tcttggccca
     3781 agcacaggct ccattgttga agaagcagct tcaagaggaa ttccatggat taggttgaac
     3841 aggcactcct tagtgcaatt agggtatgga ataaatcaac ggagaataca ggctactatt
     3901 gcaagcacta ccagcagcat agctgtagag attgcctgtg ataaagaaga cactaaaaac
     3961 ctgctcgaag cacaatctat tcctgtacca agaggaaaaa taattcgtga tgaagatggc
     4021 ttgaaagatg ctgttgaaga tttgggctat cctttagtta taaaaccatt gaatggtaat
     4081 catggtcgtg gagcaacaat aaatattgtc aattgggatc aggcagtaga ggcattagaa
     4141 atagccaaga aaatttcacg ttctgtaatt gtagaaaagt atattacagg ttatgatcat
     4201 cggattttag tcatcaacta caaatttatt tgtgcagcac aacgcaaacc tgctgcagtt
     4261 gttggcgatg gcaaaagcac aatacaacag ttggttgatg ccgtaaatgc tgatccacgc
     4321 agaggttatg gacatgaaaa tgtgctgaca tcaataaaaa ttgatgagat gacacagaat
     4381 attttagaag aaaaagaact gacccttcaa agcgttctta aaaaaggaga agagttgttt
     4441 ctgaaacgta ctgcaaacct gagtacaggt ggaacagcaa ccgatgttac cgacattgtg
     4501 catccataca atgtttttat gtgcgaacgt attgcaagaa ttattggact cgacatttgc
     4561 ggtattgaca ttatgactcc cgacatcagt gaaccactga cagaaaccgg tggtgccgta
     4621 ctggaagtta atgctgctcc cggattcaga atgcacattg cgcctacaga tggactgcca
     4681 cgtaatgttg cagagcctgt tgttgatatg ctttatcctc cgggaagtaa ttatcgcatt
     4741 ccaattattg ctgtcacagg tacaaatggt aaaacaacaa caacacgttt gattgctcac
     4801 attgcaaaaa cctgtgggca taaagttgga tttaccacta ctgatggaat atatattcaa
     4861 aatcagatgt tgcagcgtgg cgattgtaca ggtccacaaa gtgcagagtt tgtattgaaa
     4921 gatcctactg ttgattttgc ggtattagaa actgcaagag gcggaatact gcgtgccggt
     4981 cttggatttc atcgttgtga tattgcagtt gtaaccaacg ttgcagcaga tcatcttgga
     5041 cttaaaggca tcaacacact tgaagaaatg tcgaaagtta aagcagtggt tcctgaaagt
     5101 gtacaaccca atggttatgc aattttaaat gctgatgatg aattagtgtc taacatggct
     5161 cgaaatctcg attgcaaagt ggcttatttc agcctcgatg aaaacaatcc gttgataaaa
     5221 cgtcattgcg aagatggagg tcttgctgcc attcttgaaa atggatttgt aactatttgc
     5281 aaaggcacat ggaaacttcg cgtacataaa gtcattaaca ttccactcac cttttcaggc
     5341 aaagcagtgt ttatgattca gaatatatta cctgcaattc tttcagcctt tatccgcaat
     5401 tttaaaattg aagacattcg tcttgcattg gaaacattta tcccttctcc ggttcaaact
     5461 ccaggcagga tgaatatgtt tcagttcaag aaattcaatg tcatggttga ctatgcacat
     5521 aaccctgcag gcttccaagc tattgcacgt ttcctcgaaa aagttgatgc taaaccaaaa
     5581 gtaggtgtca ttgcaggtgt tggcgacaga cgcgatgaag acatcattgc attaggaacg
     5641 cttgcagcta aaatgtttga tgatattatc atcagacaag acagaaatct acgaggcaga
     5701 acagaacagg aaataattga ctttatgctt gatggaataa aagctgttga cccaacaaag
     5761 cctgttaaag taattccatt agaaaaggaa gctattgatt atgcaattaa gaatggcaaa
     5821 aaaggttcat tcattgttat atgtagcgat gttattccag atgcacttga tcaaataatg
     5881 aagtttaaag aaaaagaaga tcaatttgaa atgcatcctg acgatattcc taatcagtca
     5941 aacaggtaaa ttatattttt taagaattta aagttctttg ctgacatgat tactcgctta
     6001 tagtaatatt gttttattat cttagcatgc gatttccaat ttaatgtcgc gttttctgtc
     6061 aagaataata ttttgtgttt gtttgttttt ttcccactgt gtattatatg catcagacac
     6121 tactcctaca gcacaacaaa aacagattga tacaagaata tcagaagctg aagaaattta
     6181 caaacgagac tttaagcatg cttatgtaat tacattagaa gcaaaatctc atgcagttaa
     6241 aatcaattat ctgcaaggaa ttgttgctag ctcatgcttg cagagtataa ttgaatcaca
     6301 aacaaataaa agtattcctg caattgctgc tttaactaat ttactgcaat catcaaatgg
     6361 tattaattta aaattaaaac aacagctact atcaacttta ggtatttgtt atttttatat
     6421 tggcaaactt gattctgctc aattttattt tactgaaagc ataagttcat attcggcaca
     6481 tgacacttct attcaactct ttaattcctg tcttcaacta tcggaagttt tcatcaaaga
     6541 aggtattcct tcaaaagctc ttgaccttta tggcaaagct tataaaatat atcagcaaaa
     6601 tacgagcaag gaaggggaag cttggctgaa tttagtttta ggtaaaattt attacaggca
     6661 aaatctttat gaacaatcta ttagtaccat aaacacaact atcagtcaat ttgccagttt
     6721 gaatagtctg accggacaaa tatcttcact tattcagaat ggcaactcct actacaaaca
     6781 gatgctcgat gacagcgctg caaattgcta tcagcaagcg ctaatgtatg caacaacctt
     6841 aggtgatgaa cgcaacaaat ctatttgcta ctctaacctt agtcgcattt atctggaaca
     6901 taacaagacc aaacaagcac ttgaaacagc actacacgct cttgaggtca ttcatcccgg
     6961 aaactaccct gagatagagg caggcaccta tcagcaactt ggtgatatct atgtggaact
     7021 tggtcaaaaa agtaaagcca ttgaatggat tcagaaagca ttattgctgg caaaagacaa
     7081 taataacaaa actattgaac gcgattgcta taaatcactt tctgaactat atgcatcaat
     7141 aaagcagtat gaacctgcaa tggaaaatct acttcaggct tacagaatca aagacagcat
     7201 acaaccaatt gcatttaata acaggttggc agaaatggaa gcgcgttaca gccttgaaaa
     7261 gaaagagtca actatcaaac ttctcaatca gcaatcatta attaatgaac ttcgaataaa
     7321 agagcaaaag gaaaatatca gaaaacaaaa tgctttagtt attattctgt ttttactcat
     7381 tgcaacaatc atcattgccc tctatttcta tctaagatgg cgcaaacaag ctgagttgat
     7441 aaatcaacaa aacactatca gacaaacaga agaaaatgaa cgaatgcgaa ttgcaaagga
     7501 cattcatgat gaattgggtt ccggattaac aaaaattaaa ttcctgtcag aaattttaga
     7561 aatgaaaacc aaagaccagg agcagttaaa agaaacttta cagacaatta caggcacttc
     7621 gcatcaattg attgacaaca tgcgtgatat gatctggaat atgaatccga tgaattcaac
     7681 attagacaat ctgattgcac gtgtacgtga attctcaaca gagtacttgg aagattttcc
     7741 gatagaagta aaatatgact tgcctgaaaa tataccttca gaaaaaataa gtattgaaat
     7801 cagcagaaat attctgatga tagttaaaga atccttacaa aacatcgtaa agcattcaaa
     7861 tgcctctaag gtttcaatca ggatagaaat tcaacccgtt tttaaactaa taattactga
     7921 aaatggaata ggctttaact tgaatgaaaa gacaacagga aacgggctga gaaatataac
     7981 tgagagaagt aaagctattg gagcatcatt aaaaatatct tctaaaccgg gagaaggatc
     8041 aatactttct ttggaaaaaa tctgaatata ctactttagt agtattgcat tcactaatga
     8101 aaggtttatt tttgttttat gacaattcag gtgggtatca ttgaagacga aaagcacatc
     8161 agagagaatc tgcagatatt aattgatggc agtgaaggtt tctcctgcaa acatttattt
     8221 gcaaatgctg aagatgctat aaatatcata cctacgctgc aacttgatgt agtgctgaca
     8281 gacattcagt taggaggcaa gacaggcata gattgtattc ttgaattaaa agacaaatgc
     8341 ccgtcaacca attttctcat atgtacttca ttcgatgatt ctgagcatgt ttttaaagct
     8401 ctgaaaaccg gtgccatggg ctatctcact aaaacaacac agccatcaaa attattagat
     8461 tcaatagttg atgtttacag aggtggttca ccaatgaaca gtcatattgc acgtaaagta
     8521 gtttcaagtt ttatggaaac acctgtaagc cccaactacg aaaaattatc agagcgtgag
     8581 cgactcattg ttgatttact ttctaaaggc atgcgatata aagaaattgc cgaccaactt
     8641 tttttaagta ctgaaactgt tcgcacacac attaggaaca tttatcacaa acttcaggta
     8701 aactcaagaa ctgaagcact taacaaagta ttcaaaaaat aattactact aaatggtgat
     8761 tgactaccca taatacaggg gctaattttg tttcataaaa aaacgcaaaa tgaaacaaat
     8821 gctaccaaaa gttaccgcag tatggcttgc ttctgccatc ctctccccga ccctactcat
     8881 tgcacaaggc aatgaaacaa ctcacgaatt gtcaaagcct gcccaaaaag gcattctggc
     8941 aaatgccgaa ctaagtgctg acggcaacat cagaatgact tacaaaatga aggttgacaa
     9001 aaagagtgat caggttaatt atgaagatta tgtctttgat aaaaatctga aatttatagg
     9061 agtagaaaaa actaaagaag acaaacaaac acaacccgac caaaaagtta ctttgataaa
     9121 agcttttgtt ggtggtacca attcatttaa tgttatgagt atgtcactga atctgcaaca
     9181 ggaagtctgg gaaagaaact ggaacttcga taaacaaaaa tatcaatggg gcagccgcat
     9241 ttctaaagaa gtggtaaaac caaagaataa cgatagcaag tacagaggct ttgcggctta
     9301 tggtaatgac gatgacggta gtgtgcttat tcttgcaagt tatgatagtg gtgaagatga
     9361 tcagttcgtt tgccttcacg ttgacaaagg tttaaacatg actgaaacaa aactaccatt
     9421 acaaggtaag tactccttag tttattgtgg tacactgaaa tcaggaaata ttttcgctgt
     9481 atttgcacct aatgaaggca tgagcgacac caagaaatat tattacattg aatgtagtgc
     9541 agacagtaaa gttgcaaaaa gcagtgagtt tacttcgcca tcgcccaaca tgttgataat
     9601 ggattatagc gaagctaatg gagacttgta tttcattgcc ggatctgaca aaagcactga
     9661 ggcttataat aaagttttta ccagctatgc accaattgac aatcccggat attctacttc
     9721 caacaacaag caaatggaca aatacgaaaa acgtgctatg ggaactgaat ttgctaattt
     9781 gcatttattg agattctcag atggcaaact tgcatttgct tcgactgaac cggtaaaaga
     9841 ttttaaaaca aaaatggtta cgcctccttc acagaaaaaa ggtcaacctt ataccggtaa
     9901 gaagatagtt attgaaaatc tttttgttgc accttcaggc gatgtgcttg ttgcaggtca
     9961 gttgtctgat aaaaaagtag tgaatggcgg caactcctat gaattcagat attacgacta
    10021 tgtctgtgtg caatttgaca atcagggaaa actagtagca cagtatgctg taaacaaaat
    10081 gttagatgat agtaaaaatg aagttctttc agcaattcaa aacttcgttc cgtctgctga
    10141 tggcaaatct gtctattggg aaattttaga agttcaagct gctaaatact atgcatcagt
    10201 agcagcagca tatagtggca attactctct ttcatcgcat tactttccac gcattgccaa
    10261 tattaccttg tctaatcatt ctgtaagcaa ctttaccgag cttggtgaaa aaggaaaata
    10321 tttagtttat catagtatca ctagtctgac agacaaagac aacaaccgct tctacatagg
    10381 acatgatgaa gattataaaa acatttggtt aggcaaatac tctttccggt aataatttat
    10441 aaatcacaaa ataaaaatta aataaatgaa aaagttaatc attcccattt tatgcattgc
    10501 catattggca ttcacttctt gcaaaaagaa agacaaagac aaccctcatt acacatgcag
    10561 cagttgtgta actcaggcag aagcattagc tgcaaacgat ggaagcagca aaggtgttta
    10621 caaaggcatc atcattggat catcaggcac cataaagttt tccattcaga atggcactaa
    10681 tgatattact gcaacaatgg ttgttgatgg cgtttctgct acgctaacct ctacagtcag
    10741 ttgggttagt ggtcagcctt acattgcacc ttttacagga acattaaatg gtcaggctgt
    10801 aagcatcact ttctctgttc agaacaacgg aagtactcca caaataattt catcaaacat
    10861 tccgggtcat ccaaatgcag tatttaccat tatcaaagaa acttcaaatg cattggttga
    10921 gggttttgaa ggcacctatt ctactacaga acctgagtca ggaacgttca atattctact
    10981 ctcgcgacct ttaggtcaat ggggcggaat atcacgggaa aatggtcaga catcaaccaa
    11041 tgatgtttcc ggaactatta catcatccgg tgatttatat gatgataaca atataaaggt
    11101 tggaacactt tccggagatg agattgatgg cacatttaca gacaacagcg gcaataccgt
    11161 taccgttgat ggtcaacgta ccctataaaa aacggggatt ttattcccac ccttaaagga
    11221 gttcagcaga aaacgttctg ctgtctcctt ttatcttttt aaattctatc tgataatata
    11281 aacggtaaga aaatgtaagc ggtacacaca aatactctga attatcaaat tatcagatta
    11341 tctaatcatc taatttactt catcactctt ctcttcatca cctttcaccc ctgtcagccg
    11401 tggctgatca tcatctttca cttgtaagca ggacaatgac cacctttacc ttttttaaat
    11461 cggatgttat cgtaaatgtc actggtgtat ttaaaaccaa aataaaaatc aaggccattc
    11521 atttcataca aacccggcca ccagccaagg ttatcgctgc cgagaacaaa ccacttatat
    11581 cgaactgcaa ttcctgctct gaaaaaattg tactgatata atgacaatgg taagctcacc
    11641 tctactcttc ttgtttcaaa acgaggtgta agcgaaactt gtgaagcacg tgttacactt
    11701 ctatcgaaaa tctgaatatt ctgaatgact gccagattga gataatattt tggcgtaata
    11761 ctccagtcag cctgaaatga tgctgcggct ggaagtatca tattaaatcc actaccactc
    11821 cgcgacaagt ttgcatcacc ataaagtttg gcactaaaaa tagaatcggc atgttcatag
    11881 ccggtaatct ttgtagtatc aaatcctggc caaactgctg atgcatttct tatattaaaa
    11941 gttcttgcat taccattaaa tctaatgaag ccaaggtcaa tgagggaaag tccgagtttc
    12001 aaatcgtatt ttttcaattt aattcctgac ataccgggtt tgtaagcctg actgatgtag
    12061 ttgaaaaaat gctgtacgcc aaagctcatt gaagctcctg cacctcgtgg atgcaggtaa
    12121 ttatttttat catcattagg aattgaatga ccgtaatcaa cttcaatatt tttaatattt
    12181 aataatgacg catctgtgaa ataatagtca agtacagttg aattgatata agtaccgctc
    12241 atccccaata aaaaatttac tgtgccacca gctgttgtaa tattttgtct gtttgttaaa
    12301 attgtcttgg caaaagtaaa tccggcttcg gcaaatacca acgcccctgc actgaatgca
    12361 gttgaattcc aaggaatttt atgctgtgga ctataatcac taccctccca aagaaattta
    12421 gccaaatgat aaggtacttt tattgcactt aactgactgc gtaaagaaaa atgaattccg
    12481 aaagctgctt cctcattgcg ccatacagca gatgggcctt tgagcaacaa attaccataa
    12541 gcattcttag gtgttttatc ataatggtca ctataatcct catgacgcaa cggagcaaat
    12601 cttgcaaact ttcctgttgg aatgtctgat gccggaaagt aaatataatc attctgaaaa
    12661 aaaatatctc ctgaaagaat attaaactcg aaacgatatg gcatagttgc aatggaagca
    12721 ggattcattt caatacccat atttcctgca aaattactgt gtgcaatgcc attcatttcc
    12781 tgcgcgaaag ttagatttac actacaagaa aaaatgaata aacaagccaa tagaaactgc
    12841 ctattcagca cattaatctt aaaaaaagtt tgcaacatac tttccggttt cactttctga
    12901 attgctgcaa aattaaatat tgcatttctt aatcaacgtt aaaaattaat aagtagtgag
    12961 cctaaagttg gattatttct gcatttttgc agcatgaaga aattttattt tcttattcta
    13021 tcaatttttg ttgttacatc gtcatgcaag gagaagaaca agctaagcga tttagactgg
    13081 ataatcggaa aatgggaagg cactgcaaga aatggtaata tattctatga agaatggaaa
    13141 aaacaatcag acactgcatt tatcaataac aactaccact ttgagggtga aaaacaggtt
    13201 tttggcggaa cttccaaaat tgttttaaga aacaaccatc tttattacat caatgacagc
    13261 gacagcctta aaattctgca atgggaggcc gaagggcaca attctacttc catgactttt
    13321 tataatgaaa aaatcacacc ctataaccgc ataacttttt cactgtcaga acagaataca
    13381 tgggatgcca agcttatggg aaagaaagac acagtaaaat attctttaaa aaggaaaata
    13441 atgccttatt aaactgaata tttttttatc ctaagtttca tcagaaatga gttaaattct
    13501 gcacttttgc acttctgaat tttaaaatta tatctcaaat attatatcaa tgaaaaagat
    13561 ttttctgcta ctttttatct gtatcttaac ctattcagca ggttatagcc aaagctgttt
    13621 tacaattaac agtattcttg tagatgcctg cgggaatcct gagggtgaaa atgaaatggt
    13681 tcgttttacc gtaggtgctt ctccattaca ggtaagctca ttaaatgttt catggccatc
    13741 aaccaacctt aattttcttg gcatttgcca gaatgcaaac acagcacaaa aagtagccga
    13801 tttgaatgct actattcaat catgtggtta tctgcttgaa ccatcaaatg atattttgcc
    13861 acaaaacagt aaagtaattt tagtcaccag tgaaaacttt aatactacct tcaattcttt
    13921 tgcaaatctg acagatacga tgtatgtcat ttttcaatgc gcaggaaata cacaaggaca
    13981 tttcagaaat tattcagcaa cagttacaac accacgtata ttaacaatga actttggtgc
    14041 tgtttgtagt caaactgtac aatattttcc aaatcaatta gtaaatcaat tcggacaatt
    14101 agatacagcc gcacaagatg gctctagtgt aggatttgat tctttaggaa atccaacata
    14161 ttataacaat ggctgtcagg ctccgattat ttctttaact gctacattac aaagtagtgc
    14221 tacaacagtt tgctccggca attcagttac tttaacagca agtaatctta caggaaatta
    14281 ttcaggatat ttctggagtg gtggcaatgg cactttgagt actcctaaca atcttgtcac
    14341 aacctaccaa accaatgcaa catttacagg cacagacacc atacagtttt ccatgctcgg
    14401 ccaatgcaat gacactatta caagtaacat ttacttaatt gttcagaatg gtcagcctgc
    14461 atcaatcaca ggtccttcta ctgtttgtac aggagacagc ataacactga ctgcatcatc
    14521 aggaacagca taccaatgga gtaacggtgc cacaactcaa tctattaccg ttttaactcc
    14581 cggaacttat acagtaacag tgacaggaac atgcggaaca cctacagcaa cagcaacaat
    14641 aacaggtggc actgcaccta atattactgt tacaccggga acaaatgtca gcatttgtgc
    14701 aggtcagtcg gttactttag cagcttcagg tgcatcaagt tatcaatggt caaccggagc
    14761 aacaacatca tctatttctg taaatacagc aggaacctat actgtaagct caacaaatgc
    14821 ctgcggaaca tcatcagtga ctatcaatgt gacacaaact acaacacctg ttgtaaatat
    14881 aacctcaaat agcggaacag aaatttgtcc gggacaaagt ctcacactta cagcatcagg
    14941 tgcaagcacc tatgtttggt caggtgggca aacaactccg tcaatcacga ttaatactgc
    15001 a
//
LOCUS       DAOOOL010000058        14894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_338_length_14894_cov_183.762652, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000058 DAOOOL010000000
VERSION     DAOOOL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_14894_cov_183.762652"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PKU82_06265"
     CDS             <1..1185
                     /locus_tag="PKU82_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOZ82515.1"
                     /translation="LTFFKVLSGEVSAGTDLMNHKTNTSERLTQLFIMDGKSRNSADK
                     LKAGDLGCTLKLKNTLTNHTLCGKGITINIEPIQFPEPKSRVALIAKNKSDEEKLGEV
                     LSELHLEDPTLVVEYSKELKQVILHAQGDLHLAVTKWRIENVYKMQVEFIKPRISFRE
                     TIQKPAVATYRHKKQSGGAGQFGEVHIKIEPYYDGMPDMKEYPLRDKEEHALPWGGKL
                     VFNNCITGGAIDTRFLPSVLKGVMEKMQEGPLTGSFVRDIRVSVFDGKMHPVDSNDIS
                     FKIAGMMAFREAFHNAQPQLLEPIYDVEVEMPDDMMGDVMSDLQSRRSIIMGMDSRNG
                     RQVIRAKTPLLELDGYSTALKSITQGKAKLKTSFAEFAPVPGDLQKKLHDEYVKVEAL
                     SS"
     gene            complement(1208..1957)
                     /locus_tag="PKU82_06270"
     CDS             complement(1208..1957)
                     /locus_tag="PKU82_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ82516.1"
                     /translation="MKVLKIAFGLYAAIVFFITLLCVFPLYFLVFLFFSEKKSPVIAH
                     NLSRIWALILFKSFFIRYKIINGDFISDKGTYVFVANHRSMLDIPLYARACNNTFRFL
                     SKAELTKIPLLGYVIRNLYVTVKRGDKKDRQKSIEAMRKTLESDISIFICPEGTRNFT
                     DQMLLPFKDGAFRLAIATQTPLAVLTVINTDVLLSPESPLALSPGKLVAEWSKPIDTK
                     GMTDDDVESLKQQTAKLMQEILTRHLMSTQK"
     gene            2066..3355
                     /gene="eno"
                     /locus_tag="PKU82_06275"
     CDS             2066..3355
                     /gene="eno"
                     /locus_tag="PKU82_06275"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOZ82517.1"
                     /translation="MGNIAEIRARQILDSRGNPTIEVDVYTDNGVVGRAAVPSGASTG
                     AHEAVELRDNDKKIYLGKSVMRAVNNVNAILNKELHGYPVFDQRSIDAAMIHIDGSPN
                     KATIGANAILGVSLACARAAASESGYPLYRYIGGVNARILPVPMMNILNGGSHADNSI
                     DFQEFMIMPVGAESFSESLRMGVEIFHNLKSVLKAKKYSTNVGDEGGFAPDIKSNEEA
                     ILTVIEAIEKAGYKPGDDVMIAMDAAASEFYDAENKVYHFKKSSGEKLTSSEMVSYWK
                     QWCDKYPIVSIEDGLAEDDWAGWKELTNVLGSKVQLVGDDLFVTNTERLARGIKENIA
                     NSILVKVNQIGTLTETINAVEMAKNNSYTSVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEMLGGAAEFPGKNFRNLI"
     gene            3401..3670
                     /locus_tag="PKU82_06280"
     CDS             3401..3670
                     /locus_tag="PKU82_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82518.1"
                     /translation="MELEESDGEIKKERIRVRKRIRIKKKPDPKKKIKKYLRIFAWIV
                     VIAIFITTLIVLFSESDLRYENTKKPKSRVELNPSTKDFSVNFNL"
     gene            3702..5024
                     /locus_tag="PKU82_06285"
     CDS             3702..5024
                     /locus_tag="PKU82_06285"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HOZ82519.1"
                     /translation="MDNIKEKFKEKALPMAGTIKDIVKTHGNFVLGQYTVEQVYQGMK
                     GMIGMVTETSKLDANEGIRFRGYSIPELREKLPVSKEGPEPLPEGIFYLLLTGEMPLL
                     KDVIEVNNEWMKRATVPKHVFDMLDMLPDKTHPMTQFSMAIMALQTESVFARKYREGM
                     NKKDYWDPMYEDVMNLIARLPRIAAYIYRSTYKDGKHIEPDPKLDWAGNFAHMLGFEE
                     LDFRKLMRLYLTIHADHEGGNVSAHATHLVGSALSDPYLSLAAGMNGLAGPLHGLAAQ
                     EVARWIIELRDKYNGVPSREQLGEFIKTTLAEGKVVPGYGHAVLRKTDPRYTAQQDFA
                     KKYMADDELFKIVQLLYDVTPEILKATGKIKNPWPNVDAHSGILLMHYGLVEYDFYTV
                     LFGVSRAIGVLTSLLWDRALGMPIERPSSVTTQWIQEKIKSGDAVKAE"
     gene            complement(5053..6855)
                     /locus_tag="PKU82_06290"
     CDS             complement(5053..6855)
                     /locus_tag="PKU82_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ82520.1"
                     /translation="MAIILGISAFYHDSAATIAIDGKIISAVQEERFSRIKNDASFPV
                     NAIKFCLLHSGLKLNQIDHIVYYEKPFLKLERLLETFLETAPKGLQQFTQAAPIWIKE
                     KVLIKNKIRESLHLIDENYSAKEILFCEHHLSHAASAFFTSPFPDAAIITTDGVGEWC
                     TTSIMNGKLNSITSIKELNFPHSIGLLYSAFTQFLGFKVNSGEYKVMGLAPYGNRNSA
                     QYKLYKNKISELVDLKTDGSVFLNMKYFNYLTGLNMINENLWSELFEIKPRTAESEIN
                     LNHCNLALAIQTITENVILNLAKEAKQITNSDNLCLAGGVALNCVAVGKLNKQNIFKK
                     IYVQPASGDAGGAIGAALAVEHIFLNNKRKVPEKDLMQGCRLGPSYDNHTVTQTLKKH
                     NLHFEEFKEGELLERTARHLANDKTIAWFQGRMEFGPRALGSRSIFANPATPQIQQTL
                     NQKIKKRESFRPFAPAMLLEDYLEFYEGGNENSFMSMTDFLKKEKRLGDDFTSEETGI
                     TEQLQKSKSEIQGVTHVDYSSRIQVVSETEDPLFFKLLTEYKKITGRGILINTSFNLR
                     GQPIVCSPDDACKCFLETDIDLLVINNFIASKNN"
     gene            complement(6889..8769)
                     /locus_tag="PKU82_06295"
     CDS             complement(6889..8769)
                     /locus_tag="PKU82_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ82521.1"
                     /translation="MIRKRKPYLLFVVLIFTSCLSVKQRTADTYYEQNLYDKAIPYYQ
                     EVANKKFNLDILIKLADCYRNVKNYPLSEQIYSKIVTSNSDPIYQFYYAEALMQNGKY
                     VEARKWLNKFLVLNRADSRALNLLASCDSIEMLYKDSLLFDVTRLPFNGIDESNFSPE
                     FFHSGIVYVSNRNIDAKSYKTNPVIDNYNLFYAQQTELGHWMDPVPLRGTINSIFNEG
                     PCTFNTEFTKAYFTRNANEGKKIITNQQSENVLEIFEATTKDGEWNISGPVSFNNNNY
                     SVGHPSISPSGNTMVFVSDMPWGYGGTDIYKVQWVNNTWSKPVSIGKTINSSGNEMFP
                     YLLTDSILYFSSNGMIGVGGLDIYRTELTADGWTVPENLGYPINSPRDDFGLICDNEE
                     LSGYFSSNRQQGKDNIFSFKRNPPVLTLKGKLIDKSTNKSLKNINITLTASSIDTVLT
                     TDSEGNFSAQLKNNKEYKLRAEDKSYFTASEKISTFGYRKSATLNKDLTLEKVIFNKP
                     VIWRGIAFEKGKSDLTPSTKTELNKLFNILLENKNIKIELSCHTDSRGIERDNLILSQ
                     ARADKAAAYLINKGISKDRIISVGFGSQRLLNNCVRGVLCLEEDHQVNIRTEIRYSPY
                     QR"
     gene            complement(8772..9692)
                     /locus_tag="PKU82_06300"
     CDS             complement(8772..9692)
                     /locus_tag="PKU82_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ82522.1"
                     /translation="MKKLYSLIITLFMSGWLFGQQAPMTTQYMFNTLLINPAYAGSKP
                     FTSATMMVRKQWAGFKGSPTTETASIHGALEDKNVGLGLYISNDHAGITNRTELFGSY
                     SYTIEYGESKLAFGLQAGLSYLKSTLSDLVYNDNNDPVYDKNTVNNVLPNFGAGLYYH
                     TDLFYAGISAPLLLSYDPLKTLSVEMTDVHHIRRHYFLTTGYVFSLSEQVKLKPSVLL
                     KYVNGAPLQYDINANLLLNNIIWIGTSYRSNDALAIIGEYQLSKKLRIGYSFDYSLTI
                     LRNHSAGSHEFMLGYDFGYNVPKIKTPRYF"
     gene            complement(9697..>14894)
                     /locus_tag="PKU82_06305"
     CDS             complement(9697..>14894)
                     /locus_tag="PKU82_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ82523.1"
                     /translation="GTYTVTVTDSTGCVGNKTQVVSQPTNIVVTLTPHNATCAQSNGW
                     ISSAVTGGTSPYTYLWSNGASTSSITGLLPGNYTVTVTDAALCTKTASQNITTISGPV
                     VTVDSVKNARCFGQANGAVYISVSGNNGPVTYLWSNAAITQDIVNVVAGTYTVTVTDS
                     TGCVGNKTQVVSQPTDIAVTLTPHNATCAQSNGWISSAVTGGTSPYTYLWSNGASTSS
                     ITGLLPGNYTVTVTDAALCTKTASQNISTISGPVVTVDSVKNARCFGQANGAVYISVS
                     GNNGPVIYLWSNAAITQDIVNIVAGTYTVTVTDSTGCVGNKTQIITQPSALTLIDTIV
                     PARCGNANGSAGVNVTGGTSPYTYLWNTGQSTASISGLNGGSYTVTVTDSKLCTANRT
                     LIVSNTPSLVISIDTIIHVKCSGQNTGAIKVNVTGGSGPLTYTWTPTQIGTDSIFSLA
                     AGTYKLVVRDTANCRDSINVTINSPSAINSAIYSLPETCGINNGKAWVTVSGGTPGYT
                     FLWNNSSTNDTISNLTKGTYSVTIKDANNCSVNKNILVDSLGGPAITDSIIHVKCFGE
                     NTGAIYTTVHGANGPVSYLWSPGGQITQNINGLTAGTYTVTVTDTNSCSSTKSFIISQ
                     PTSLNISFTSVTEKCNQANGSAIAAVIGGTLPYSYQWNNGNTTNNIINLTTGYYTITI
                     TDGNLCTKRDSVFIGNTNGPSISNFSRNLIKCHNDSSGSLTVNVTGGTPFYNYQWSGF
                     PGVNNATLQNIPAGVYTVTVTDANGCSVNATDSLQNPPLMTITHTLSNASCNLNNGIA
                     QINVSGGAAPYTYSWSNGVTGVATLNNLSNIFYTVTITDNIGCIKTDTFTIQRTPATF
                     VQLTSLNNVTCYGLSNGSITITPSGGDGNYTYQWSYNNIQTQNLNNIPAGSYDVTVTD
                     GTGCTYTMPDIQVFQPDTLIAQVITLPTGCTSQTGSASVSQTGGTQPYTYLWSTGGTT
                     QTINGLSSQIVTVTVTDNNGCTTTTSGIINALSAPVINISGIQNINCFGGGNGFINTT
                     VTGGNLPYNYLWNNGSTTSNISNLSAGTYTLTVTDSAQCSTIITQTLTENPQIQFGLT
                     LIAGPCNGGTPAIAISAPSGGVSPLNILWSDGSTSTSAQATAGTVMTLTVTDNAGCNR
                     DTTFSMLSTNGPQISSIQTTDIICFGDSSGSATVNITGGTPPISIQWIGTLPVQNTAT
                     AINLKAGTYTCIVTDVNGCSAQSVANINHLNTLITPVLAITNASCGNSNGQIVVTATG
                     GSGTYQYKLNNGVYSATNTFTNLAAGSDSVFVKDNNGCEVFVISTISNIQGPVISVID
                     SANVSCFGSNDGFIILNVTGAAQPITYSWSDGPTTLTRNNLGPGNYTFTAQDANNCIS
                     VRNFIITEPTAITISEIIPNKNGIYNITCNTLADGEIQVIANGGVPFTSTTAYNYQWS
                     ISGASGANVINLTAGPYTVTVTDSMGCSVTKSFLLTEPPALNAGPDFTQSICGTDTIV
                     LTGNIPTYGFGKWTVRSGGGLIAEPDSITTQITAIANGVNVFAWVVYDNFCSDTALYV
                     INKKEKIIADGGIDQNVCDNFVFLAAVPPPSGTGVWQSINGNSTIQNPSSPATKVENL
                     SPGKNIFVWTITNGSCTASDTVKIAILPPEECYTDVVMPTGFTPNEDGYNDIYVIRGL
                     DNTDNTLTIFNRWGNLVYEKKNYSNDWDGKSNNKQILPDGTYYAILRIPARNLVLKTY
                     IDLRR"
BASE COUNT         4622 a         2596 c         2867 g         4809 t
ORIGIN      
        1 ttgacctttt ttaaagttct ttctggagag gtaagtgcag ggacggattt aatgaatcac
       61 aaaacaaata caagcgaaag attgacccag ttgtttatca tggatggaaa gagtagaaat
      121 tcagctgata aacttaaggc aggcgattta ggatgtactc ttaaattgaa gaatacactt
      181 acaaaccata cgctttgcgg aaaaggtata actattaata ttgaaccaat acaatttcct
      241 gaaccaaaat ccagagtagc actcattgca aaaaacaaaa gcgatgaaga aaaattgggc
      301 gaggtgctga gtgaattaca tcttgaagat cctacattag tagtagaata ttctaaagaa
      361 ctcaagcagg ttattttaca tgcgcaaggc gatttacatc ttgcagtaac aaaatggcgg
      421 attgaaaatg tatataaaat gcaggttgag tttataaaac cacgcatatc gttccgcgaa
      481 acaattcaaa aacctgcagt tgcaacttac agacataaaa aacaatcagg tggagcaggt
      541 caatttggtg aagtgcacat aaaaattgaa ccttactatg atggtatgcc tgatatgaaa
      601 gaatatccac ttcgtgataa agaagagcat gcattaccct ggggtggaaa attagttttt
      661 aataattgta ttacaggagg tgctattgat acacgctttt taccttcagt tcttaaaggc
      721 gtaatggaaa aaatgcagga aggtccgtta acaggatcgt ttgtgcgtga tataagagta
      781 agtgtgtttg atggtaaaat gcatcctgtt gattcaaatg atatatcatt taagattgca
      841 ggcatgatgg catttcggga agcatttcac aatgcacagc cacaactttt agaacctatt
      901 tatgatgttg aagtagaaat gcctgatgat atgatgggcg atgtaatgag tgacctgcag
      961 tcgagaagga gtatcattat gggtatggac tctcgaaatg gaagacaggt aattcgtgca
     1021 aaaactcctt tgcttgaact tgatggttac agcactgcat taaaatccat cacacaagga
     1081 aaggctaagt taaaaacatc atttgctgaa tttgctcctg tgcccggaga cttacaaaag
     1141 aaattacacg atgaatatgt gaaagtggaa gcactaagca gttaaaatta atcaacaaaa
     1201 acaaaaatta tttttgtgtt gacattaaat gacgtgtcaa tatttcttgc atcagttttg
     1261 ctgtctgttg ttttagtgat tcaacatcat catcagtcat tcctttggtg tcaataggtt
     1321 tactccattc tgctaccagt ttacccggac tcaaagctaa tggtgattca ggtgacaaca
     1381 atacatctgt atttattact gtgagtactg ccaaaggtgt ttgtgttgca atagccagac
     1441 gaaatgcacc gtctttaaat ggcagcaaca tctgatctgt aaaattacga gtgccttcag
     1501 gacaaataaa tattgaaata tccgactcaa gtgttttacg cattgcctca atactttttt
     1561 gtctgtcttt tttatcacct cgttttacag tgacatacag atttcggatt acataaccca
     1621 gtaacgggat tttagtcagt tcggctttag ataaaaatct gaaggtattg ttacatgcac
     1681 gcgcatagag tggaatatcc agcatagagc gatggttggc aacaaataca taggttcctt
     1741 tatccgaaat aaaatcacca ttgattatct tgtagcgaat aaaaaaactt ttgaacaata
     1801 tcaatgccca aatacgagaa aggttatgtg ctataacagg agatttcttt tcactgaaaa
     1861 ataaaaatac taaaaaatat agagggaaaa cacaaagcaa agtgataaaa aatacaattg
     1921 ctgcatataa tccaaatgca attttaagca ctttcatata gcgctattag tgttatagat
     1981 gtggttgaaa tttatttcgc taaattaaat aaatttacgg cacattacgt taactttgca
     2041 gccgttttat aaagtgtaaa atattatggg caatattgct gaaatcagag caagacaaat
     2101 actcgactca agaggaaatc caactattga agtagatgta tatacagaca atggtgtagt
     2161 gggcagggct gctgtacctt caggtgcatc aaccggagca cacgaagccg ttgagttgcg
     2221 tgataatgat aagaagattt atcttggcaa aagtgtgatg agagctgtaa ataatgtaaa
     2281 tgccattttg aataaagaac tgcatggtta tcctgttttt gatcagcgca gtattgatgc
     2341 ggcaatgatt catatagatg gttcacctaa taaagctaca ataggtgcca atgctatttt
     2401 gggtgtttca ttagcatgtg caagagcagc tgcaagtgag tccggctatc cgctatatcg
     2461 ttatatcggt ggtgttaatg cacgtatatt gcctgtgcca atgatgaaca tcttaaatgg
     2521 aggatctcat gctgataaca gtatagactt tcaggaattt atgattatgc ctgttggagc
     2581 agaaagtttt tctgaatcac tacgaatggg tgttgaaata tttcacaatc tgaagagtgt
     2641 tttaaaagct aagaaatatt ctaccaacgt tggtgatgaa ggcggttttg cacctgacat
     2701 taaatctaat gaagaagcaa tattaacagt gatagaagca atagaaaagg caggctacaa
     2761 acccggtgat gatgttatga ttgcaatgga tgctgctgct tctgaatttt atgatgcgga
     2821 aaataaagtg tatcatttta aaaagtcaag tggtgaaaaa ctgacatctt cagaaatggt
     2881 tagctactgg aagcaatggt gcgataaata cccgattgtt agtattgaag atggacttgc
     2941 agaagacgat tgggcaggat ggaaagaatt aaccaatgta ttaggcagta aagtgcagtt
     3001 agtaggtgac gatttgtttg tgacaaacac tgaaaggctt gcaagaggaa taaaagaaaa
     3061 tattgccaac tcaatattgg taaaagtgaa tcagattgga actttaacag aaacaatcaa
     3121 tgctgttgaa atggcgaaga acaacagcta tacatcagtg atgagccacc gttcaggcga
     3181 aactgaagat acaactattg ctgatttggc agtggcatta aatacaggac agataaaaac
     3241 cggttcggca tcgcgttctg acaggattgc caagtacaac caactcctgc gaattgaaga
     3301 aatgctgggt ggagctgcag aatttcccgg aaaaaatttc aggaatttga tttaatccaa
     3361 aaggggctgt atctttagaa tctgaattaa caacttgaaa atggaattgg aagagtcaga
     3421 tggcgaaata aaaaaagaaa gaatcagagt tcggaaacga attagaatta aaaagaaacc
     3481 tgatcctaag aagaagatta aaaagtattt gcgcattttt gcatggattg tagttattgc
     3541 catttttatt acaacattaa tagtgttgtt ttcagaatca gaccttcgtt atgaaaatac
     3601 caagaaacct aaatcaagag tagagttaaa tccttcaaca aaggacttct ctgtaaactt
     3661 taatttgtaa caaacgttta agtaacagtt atacaacaag tatggacaac ataaaagaaa
     3721 aattcaaaga gaaagccttg ccaatggcag gtacaattaa agacattgta aaaacccacg
     3781 gcaattttgt tttgggtcaa tatacagtag agcaggttta tcagggaatg aaaggaatga
     3841 taggcatggt aactgaaacc agtaaacttg atgctaatga aggaattcgt ttcagaggat
     3901 attctattcc tgaacttcgt gaaaaactgc cggtatctaa agaaggtcct gagccattgc
     3961 cagaaggaat tttttacctc ttgctcactg gcgaaatgcc attactgaag gatgttattg
     4021 aagtgaataa tgaatggatg aaaagagcaa ccgtgcctaa acatgttttt gacatgttgg
     4081 atatgttgcc tgataagaca catccaatga cgcaattttc aatggcaatt atggctttgc
     4141 aaacggagtc tgtttttgca cgtaagtatc gcgaaggaat gaataagaag gattattggg
     4201 atcctatgta cgaagatgta atgaatctta ttgcacgtct tccgcgaatt gctgcttaca
     4261 tctatcgttc aacttataaa gatggcaaac acattgagcc tgatcctaaa ctagactggg
     4321 caggaaattt tgctcacatg ctaggatttg aagaacttga tttcaggaag ttgatgcgat
     4381 tatatcttac cattcatgcc gatcatgagg gtggaaatgt ttcagcacat gcaacacatc
     4441 ttgttggctc tgcattaagt gatccatatc tttcattggc agcaggtatg aacggacttg
     4501 ccggtccgct tcatggttta gcagcacagg aggttgcccg ttggattatt gaattacgtg
     4561 ataagtataa tggtgttcca tcgcgcgagc agttaggtga gtttattaaa acaacattag
     4621 cagaaggaaa agttgttccc ggttacggac atgccgtact gcgtaaaacc gatccccgtt
     4681 atacggcaca gcaggatttt gcaaagaaat atatggcaga tgatgaactg tttaaaatag
     4741 ttcaattatt atatgatgtt acaccggaaa ttttaaaagc taccggtaag attaaaaatc
     4801 catggcctaa cgttgatgca cattcaggca tcttgctgat gcattatggt ttggtggaat
     4861 atgattttta tactgttttg tttggtgtat caagagctat tggtgttctg acttcacttt
     4921 tatgggatcg tgctttaggt atgcctattg aaaggccaag ttctgtaaca acacaatgga
     4981 ttcaggagaa aattaaatct ggcgatgctg taaaggcaga ataactgctg tttatttcag
     5041 agcgaatttg gcttagttgt ttttactggc aataaagtta tttattacca ataaatcaat
     5101 atctgtttcg agaaagcatt tgcaggcatc atcagggctg caaacaattg gttgtcccct
     5161 tagattgaaa ctggtattaa tgagtattcc tcttcctgtt atctttttat attctgtaag
     5221 aagtttaaaa aacaagggat cttctgtttc agataccacc tgaattcgtg aactgtaatc
     5281 aacatgtgtt actccctgaa tttcactttt agatttttgc aattgttcag ttattcctgt
     5341 ttcttctgaa gtaaagtcat caccaagtct tttttctttt ttaaggaaat cagtcataga
     5401 cataaaacta ttttcattac ctccttcata aaattctaag taatcttcaa gtaacattgc
     5461 aggagcaaag ggcctgaatg attcacgctt tttaattttt tgatttaatg tttgttgaat
     5521 ttgcggtgtt gcagggtttg caaaaatact ccttgatcca agtgcacgcg gtccaaattc
     5581 cattcgcccc tgaaaccatg ctatagtttt atcatttgca agatgtctgg cagtacgttc
     5641 taaaagctca ccttctttaa attcttcaaa gtgtaaattg tgttttttca aagtctgtgt
     5701 aacagtatga ttgtcgtaag aaggacctaa acgacaacct tgcattaaat ctttttcagg
     5761 aactttacgt ttattgttta aaaatatgtg ctctactgca agtgcagcgc ctatggcacc
     5821 acctgcatca cctgaggcag gttgaacata aatttttttg aaaatatttt gtttgttgag
     5881 tttgccaact gcaacacaat ttaatgccac tccacctgcc aaacataaat tgtctgaatt
     5941 tgtaatttgt ttagcttctt ttgctaaatt taaaattaca ttttcagtaa tcgtttgaat
     6001 tgccaatgct aagttgcaat gatttaaatt tatttctgat tcagcagtgc gtggttttat
     6061 ttcaaaaagc tcagaccaaa ggttttcatt tatcatgttt aaccctgtca ggtaattaaa
     6121 atatttcatg ttcagaaata cagaaccgtc tgtcttcagg tcaactaatt cgctgatttt
     6181 attcttgtat aatttatatt gtgcactatt cctattccca taaggtgcca agcccattac
     6241 tttatattct cctgaattaa ctttaaaacc aaggaactgt gtaaatgcac tatataataa
     6301 tcctattgaa tgtggaaagt taagttcttt tatagaggta atagaattta gtttgccatt
     6361 cataattgaa gtagtgcacc attctccaac accatctgta gttattattg ctgcatccgg
     6421 aaaaggagag gtaaagaaag cacttgcagc atgagataga tgatgctcgc agaatagaat
     6481 ttcttttgct gaataattct catctatcaa atgcaaactt tctctgattt tattttttat
     6541 cagaactttt tcttttatcc atattggtgc agcctgtgta aattgttgca agcctttagg
     6601 tgctgtttca agaaaagttt ccaataacct ctctaacttc aaaaaaggtt tttcgtaata
     6661 tacaatatga tcaatttgat ttaactttaa accggaatgc agcaagcaaa attttattgc
     6721 attgacagga aaagaggcat catttttaat tcttgaaaag cgttcttcct gtactgctga
     6781 gattatttta ccatcaatag caatagttgc tgccgagtcg tgataaaatg cggaaatgcc
     6841 taatatgatg gccatctttg cagaaaaata tgttctgtct attaagtctt atcgctgata
     6901 aggactgtat cttatttcag ttctgatatt aacctgatgg tcttcttcaa gacataatac
     6961 accccttaca caattgttta ataatcgttg ggatccaaat ccaactgaaa taattctgtc
     7021 ttttgagatt cctttattga taagatatgc agcagcttta tctgctcttg cctgtgataa
     7081 tatcaggtta tcacgttcta tgcctcttga gtctgtgtga cagctcaatt caatttttat
     7141 atttttattc tctaataaaa tgttaaacag tttatttaat tctgttttag ttgacggtgt
     7201 taaatctgat tttccttttt caaatgcaat gcctctccat ataactggct tgttgaaaat
     7261 cactttttcc aaagttaaat ccttattcag tgttgctgat tttctataac caaacgtaga
     7321 tatcttttct gatgctgtaa aataactttt atcctcagca cgaagtttgt attctttatt
     7381 gttttttaat tgcgcagaaa aattgccttc gctatctgtt gttaaaacag tatcaattga
     7441 tgaagcagtt aacgtaatat tgatgttttt tagagacttg tttgttgact tgtcaatcaa
     7501 tttgcctttt aacgttaaaa ccggaggatt tcttttaaat gaaaaaatat tgtcttttcc
     7561 ttgttgcctg ttactactga aataaccact taattcttca ttatcgcaaa tcaaaccaaa
     7621 atcgtctctt ggactgttaa tcggatagcc taggttttct ggtacagtcc atccatcagc
     7681 agttaattct gttcggtaaa tgtccagacc tccaacacca atcataccgt tgcttgaaaa
     7741 gtaaagtatt gaatcagtca gtaagtaagg aaacatttca tttccacttg aattaattgt
     7801 cttgccaata ctaaccggtt tactccaagt attattaacc cattgtactt tataaatatc
     7861 cgtaccccca taaccccatg gcatatcaga aacaaagacc attgtgttgc cactgggtga
     7921 aatagatgga tgccctacac tgtaattgtt attgttgaaa ctaacaggcc ctgaaatatt
     7981 ccattcacca tctttggttg tagcttcaaa aatttctaaa acattttcac tttgctgatt
     8041 agtaataatt tttttccctt cattggcatt gcgtgtaaag taagctttag taaattcagt
     8101 gttgaatgtg catggccctt cattaaaaat agaattaatt gtgccccgta aaggaaccgg
     8161 atccatccag tgacctaatt cagtttgttg tgcataaaat aaattgtaat tatcaattac
     8221 cggatttgtc ttataacttt ttgcatcaat atttctgtta cttacataaa caattccgga
     8281 atgaaaaaat tcgggtgaga aattgctttc atcaattccg ttaaaaggca atcttgtaac
     8341 atcaaagagc aatgagtcct tgtatagcat ttcaatagaa tcgcatgatg ctaataagtt
     8401 taacgctctg ctgtctgcac gatttaaaac aagaaatttg tttaaccatt ttcgggcttc
     8461 aacatatttg ccattttgca ttaatgcttc agcataataa aattgatata tcgggtcact
     8521 atttgaagtt actatcttcg aataaatctg ttctgataat ggatagttct ttacattgcg
     8581 ataacaatct gcaagtttaa tgaggatatc taaattaaac tttttgtttg caacttcctg
     8641 ataatacgga atcgctttgt catataagtt ctgctcataa taggtgtctg ctgttctttg
     8701 tttgacagat aagcatgatg tgaatatcag aactacaaat aataaatagg gcttgcgttt
     8761 tctaatcata attaaaaata acgcggtgtt ttaattttag gtacgttata accaaagtcg
     8821 tatccaagca taaattcatg cgatcctgcc gaatggtttc tgagaatggt aagcgaataa
     8881 tcaaaggaat aaccaatgcg tagtttttta gatagctgat attctccaat aattgcaagt
     8941 gcatcattgg agcggtatga agttcctatc cagatgatat tgttcaaaag cagatttgca
     9001 ttaatgtcat actgtaatgg ggcaccatta acatatttta gcagaacaga aggttttaat
     9061 ttcacctgct cacttaatga aaaaacataa ccggttgtta gaaaatagtg tcttctgata
     9121 tgatgtacat cggtcatttc aactgacaaa gtttttagtg gatcgtaact taaaagtaat
     9181 ggtgcagaaa tgcctgcata gaataggtcg gtgtgataat atagacctgc gccaaaattt
     9241 ggcaacacgt tatttacagt attcttatca tatacagggt cgttgttgtc gttataaaca
     9301 agatcagata gtgtggattt cagataactc aaacctgcct gaagaccgaa tgcaagtttt
     9361 gattctccgt attcaatagt ataagaataa cttccaaaga gttctgtgcg atttgtaatg
     9421 cctgcatgat cattacttat atacaagcct aagccaacgt ttttatcttc caacgcacca
     9481 tggatactgg cagtttctgt tgtcggtgat cctttaaatc ctgcccattg ttttctaacc
     9541 atcattgttg cactggtgaa tggtttactt cctgcataag ccggatttat cagcagtgta
     9601 ttaaacatat attgtgttgt cattggtgct tgttggccaa acaaccagcc tgacataaag
     9661 agtgtaatta ttaatgaata taattttttc ataagcttat cttcttaaat caatatatgt
     9721 ttttaagact aagtttctgg caggtatccg taaaatggca tagtaagttc catcaggtaa
     9781 gatttgtttg ttgttagatt taccatccca gtcgttacta tagtttttct tttcataaac
     9841 taaattgccc catctgttaa aaatggtcaa agtattatct gtattatcta atcctctgat
     9901 aacgtatata tcgttataac catcctcatt cggtgtaaaa ccagttggca taacaacatc
     9961 agtataacat tcttccggag gaagaatggc aatctttact gtatcggatg cagtacatga
    10021 accatttgtt attgtccaaa caaaaatatt tttacccgga cttaaatttt caaccttagt
    10081 cgcaggtgat gatggatttt gaatagtact atttccattg atgctttgcc atacaccagt
    10141 acctgaaggt ggtggtactg ctgcaaggaa tacaaaattg tcgcacacat tttgatcaat
    10201 gccaccatct gctataattt tttctttttt gttgatgaca tataatgcag tgtctgaaca
    10261 gaaattgtca tagactaccc atgcaaaaac atttacccca tttgcaattg cagtaatctg
    10321 tgttgtgatt gaatccggtt cagcaattaa tccacctcca ctacgaacag tccattttcc
    10381 aaatccataa gtagggatat ttcctgtaag tacaattgta tcagtaccac aaatagattg
    10441 tgtaaaatca gggcctgcat ttaaggcagg aggctcagtt aataggaatg attttgtaac
    10501 agaacatccc atagaatctg ttacagttac agtataaggt cctgcagtaa gatttatcac
    10561 attagctcct gaagcgccac tgattgacca ttgatagttg taagcagttg ttgacgtaaa
    10621 tggaacacct ccatttgcaa tgacctgaat ttctccgtca gcaagtgtgt tgcaagttat
    10681 attgtaaata ccgtttttat ttggaattat ttcactgatt gtaatggctg taggttctgt
    10741 aatgataaag tttcttaccg atatacaatt gtttgcgtcc tgcgctgtaa atgtatagtt
    10801 gcctggtcca agattatttc ttgttaaagt tgttggtcca tcagaccatg aataggttat
    10861 aggctgtgct gcaccggtaa catttaaaat aatgaaacca tcattgctgc caaagcagct
    10921 tacattagcc gagtcaataa ctgatattac cggcccttgt atattactta tagtcgaaat
    10981 aacaaatact tcacagccat tgttgtcttt aacaaaaacc gaatcagatc ctgctgcgag
    11041 gttagtaaat gtatttgttg cagaatagac tccattatta agtttgtact gataggtacc
    11101 actaccaccg gttgcagtta caacaatctg tccgttgctg tttccacaac ttgcatttgt
    11161 tatagcaagc actggagtta tcaatgtatt caggtgattg atatttgcaa cactttgtgc
    11221 tgaacaacca tttacgtctg ttacaataca ggtataagtt ccggctttta aatttatagc
    11281 cgttgcagta ttttgaaccg gtaaagtgcc tatccattga atgcttattg gaggtgttcc
    11341 tcctgtaata ttgactgttg cgctgccgct actgtctcca aaacaaatga tgtctgtcgt
    11401 ttgaatactt gaaatttgcg ggccgttggt tgataacata ctgaatgtag tatctctatt
    11461 acatcctgca ttgtcggtaa ctgttaaggt cattactgtt cctgccgtag cttgtgcaga
    11521 tgtggaagtg ctgccatctg accacaatat atttaatggc gatactccac ctgaaggtgc
    11581 tgatatggcg attgcaggag ttcctccgtt acatgggcct gcaattaaag ttaatccaaa
    11641 ctgaatttgt ggattttctg taagagtttg agtaataatg gtactgcact gcgcagagtc
    11701 cgtcaccgtt agtgtgtagg tccctgctga aaggttgctg atatttgacg ttgttgagcc
    11761 gttattccag aggtaattgt atggtagatt tccccctgtt acagttgtgt ttataaaacc
    11821 attcccacct ccaaaacaat tgatgttttg aataccggaa atatttataa caggagctga
    11881 taatgcattg ataattccgg aggttgttgt cgtacatcca ttattatcag tcaccgtaac
    11941 agttacaatc tgacttgata atccattaat agtttgtgtg gtgccgcctg ttgaccataa
    12001 ataggtgtaa ggttgagtac cacctgtctg acttacactg gcacttcctg tttgtgaagt
    12061 acagcccgtt ggtaatgtga taacttgtgc aattaaagta tctggctgga aaacctgaat
    12121 atccggcatt gtgtaagtac atcctgttcc atcagtcact gtaacatcat acgagcctgc
    12181 aggaatgtta tttaagttct gagtttgtat attgttgtat gaccattgat aagtataatt
    12241 tccatcgcct cctgaaggtg tgatggttat acttccatta ctaagtccat agcaagtaac
    12301 attatttaat gatgtaagct gtacaaatgt tgcgggagtt cgttgtatag taaatgtatc
    12361 tgtcttaata caaccaatat tgtctgtgat agttacggta taaaaaatat tagataagtt
    12421 atttaaagtt gcaactccgg ttactccatt actccaactg taagtgtacg gtgcagcacc
    12481 tccggaaaca ttaatctgtg caataccgtt atttaaatta caagaggcat tggagagtgt
    12541 atgagtgatt gtcattaaag gaggattctg aagactgtcg gttgcattta ctgaacaacc
    12601 attagcatca gttacagtta ctgtataaac tcctgcaggt atattctgta gtgttgcatt
    12661 attaacaccg gggaatccac tccattgata gttgtaaaat ggtgtgccgc cagtaacatt
    12721 aacagtcagt gaaccactgc tatcattatg acattttatt aaattacgac tgaaatttga
    12781 tattgatggg ccatttgtat ttccgataaa aacagaatct ctttttgtac acagatttcc
    12841 gtcagttatt gtaattgtgt aatatcccgt tgttaaattg atgatgttat ttgtagtatt
    12901 accattgttc cattggtagg agtaaggtag tgttcctcca attactgcgg caatggcaga
    12961 accgtttgcc tgattgcatt tttcagttac tgaggtaaaa gaaatattca aacttgttgg
    13021 ttggctaata ataaaacttt tggttgaaga gcaactattg gtgtctgtaa ctgtaacagt
    13081 ataagtgcct gctgtcagac cgtttatgtt ttgtgtgatt tgaccaccag gagaccataa
    13141 ataactaacg ggaccattgg ctccatgaac agtcgtatag attgctccgg tgttttctcc
    13201 aaaacatttt acatgaatta ttgaatcagt aattgccggt ccaccaagtg aatcaaccaa
    13261 tatgttttta ttgacactac agttgtttgc atcttttata gtgacagaat aagtgccttt
    13321 tgttaaattc gaaatggtgt cattggttga actgttattc cataagaaag tatatcccgg
    13381 tgttcctcct gaaacagtca cccaagcctt accattatta attccacatg tttcgggtaa
    13441 tgagtaaatg gctgagttaa ttgctgaagg actatttatt gtcacgttta ttgagtctct
    13501 gcaatttgca gtatctctaa ccactaattt ataggtaccg gccgccagac taaaaattga
    13561 atcagtgcct atttgtgtcg gtgtccatgt ataagttaaa ggtcctgagc cacctgtaac
    13621 atttacttta atagcacctg tgttttgtcc tgaacatttt acatgtatga tagtgtcaat
    13681 agaaataacc aaagaaggtg tattgctaac aataagtgtg cgatttgcag tacaaagttt
    13741 actgtcggtt acagtaaccg tgtagctacc accgtttaat cctgatattg atgctgtact
    13801 ttgtcctgta ttccagagat aggtatatgg tgaagtgccg cctgtgacat ttactcccgc
    13861 actaccattt gcatttccac aacgtgccgg tactatagtg tcaattaatg ttaatgccga
    13921 aggctgtgtt attatttggg ttttatttcc aacacatcct gttgaatcag taacggtaac
    13981 ggtataagtt cctgcaacaa tgttaacgat gtcctgagtg attgcagcat tactccataa
    14041 atagataaca ggtccattgt ttcctgaaac agaaatataa actgcaccat tagcctgacc
    14101 aaagcagcga gcatttttaa cagagtcaac agtcaccaca gggccactga tggttgaaat
    14161 attttgtgat gcagtcttag tacaaagagc agcatcggta acagtcacag tataatttcc
    14221 cggcaacagt cctgtaattg aagaagtaga agcaccattg ctccagagat aagtataagg
    14281 agaagtgcca ccggtaacag cagagctaat ccaaccatta ctttgcgcac aggttgcatt
    14341 gtgtggagtt agcgtaactg caatgtctgt tggttgcgaa acaacttgag ttttatttcc
    14401 aacacatcct gttgaatcag taacagtaac ggtataagtt cctgcaacaa cgttaacgat
    14461 gtcctgagtg attgcagcat tactccacag atatgtaaca ggtccattgt tacctgaaac
    14521 ggaaatataa acagcaccat tagcctgacc aaaacagcga gcatttttaa cagagtcaac
    14581 agtcaccaca ggaccactga tagttgtaat attttgtgat gctgtcttag tacacagtgc
    14641 agcatcggta acagtcacag tataattgcc aggcaacagt cctgtgattg acgaagtaga
    14701 agcaccgttg ctccacagat aggtgtaagg cgaagttcca ccggtaacag cagaactaat
    14761 ccaaccatta ctttgagcac aggttgcatt gtgtggagtt aacgtaacaa caatgtttgt
    14821 tggttgcgaa acaacttgag ttttatttcc aacacatcct gttgaatcag taacagtaac
    14881 ggtataagtt cctg
//
LOCUS       DAOOOL010000059        14708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_345_length_14708_cov_95.454037, whole genome shotgun sequence.
ACCESSION   DAOOOL010000059 DAOOOL010000000
VERSION     DAOOOL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_345_length_14708_cov_95.454037"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..632
                     /locus_tag="PKU82_06310"
     CDS             183..632
                     /locus_tag="PKU82_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOZ82524.1"
                     /translation="MTLIKFKNEPASRMMDRSPMFSDLFNDFFDGVVSNNITKSFSPS
                     VNVKESDHEFKLELAAPGLSKDDFKINIENDMLNISAEKKEEQATQNEKYTHKEFSYS
                     SFKRTFTLPETADREKISAAYDNGVMTLVIPKKDEARPKPLREIKIS"
     gene            complement(693..1385)
                     /locus_tag="PKU82_06315"
     CDS             complement(693..1385)
                     /locus_tag="PKU82_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOZ82525.1"
                     /translation="MTAFIILFLLITGGGLLYFLLNKSNDSLLKLSLAFSGAYLFGIT
                     ITHLVPEVFSQGDKSIGIYVMTGFLFQIILEYFSKGIEHGHIHLHEHKQHAVFPVSMM
                     ISLCIHAFFEGVPMADNQQRQSLMMGIAMHHIPVAFALMSMLMQSGVSKTVSVFSLVV
                     FAAMSPVGAFFGIYLGDTLMTEWFSKIMAIVIGIFLHISTTILFESDSNHRFNFIKMA
                     VILAGVGLSLMV"
     gene            complement(1382..2161)
                     /locus_tag="PKU82_06320"
     CDS             complement(1382..2161)
                     /locus_tag="PKU82_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ82526.1"
                     /translation="MRLSKIAINLPAEERYFMQKWFQSWFDSPYYHILYKDRDNQEAE
                     YFMDNLLEQIHLPVKSSVLDIACGKGRHSLFLADKGYHVTGIDLSEQSIAYCKQFERQ
                     SLEFYVHDMREIFRTNCYDVALNLFTSFGYFNSDHDNRLALRTASLALKPGGYFILDF
                     FNPDFIKNTSQQHIKQVDGIDFSINKHSDGNTIEKTIQFSDSGKSFKFTESVQIISKH
                     TFLKHFERVGLVTRHIFGDYSLRPFEEDSSERMIFITQKPQ"
     gene            2172..3299
                     /gene="corA"
                     /locus_tag="PKU82_06325"
     CDS             2172..3299
                     /gene="corA"
                     /locus_tag="PKU82_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOZ82527.1"
                     /translation="MAKIRRLSVKLSRKKTTHPGAQPGSFSIPSDALTPKALCFIYDK
                     DSFQEIDVTQFDQLKTAYQNLGEKTLWLHVAGFGDKTFYENLTALFQLHRLELEDVFN
                     VYQRPKVDEYEGHLFFVSRLLSMTDETLNNDQLSLILGKNFVITIQEKYQDFFDPIRL
                     RLRNGKGNMRTYRPGYLAYALMDAVIDHYYPALEKIGLKLDDLEDELLSNPSQDSMNR
                     ILQIKRELIVFRRAIWPERDKINDILRSSFDEVPEETKLFFRDSYDHCIQILDIVESY
                     KEVTASLMDVYLSSVSHKLNQVMKVLTIISTIFIPLTFIVGLYGMNFQLANPETGERL
                     PMNMPELYSPYGYVGVIAVMVLIVVIQLYIFHRKGWIRFRE"
     gene            3296..5773
                     /gene="priA"
                     /locus_tag="PKU82_06330"
     CDS             3296..5773
                     /gene="priA"
                     /locus_tag="PKU82_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOZ82528.1"
                     /translation="MTEKSSSGFYIKVVVPLPLPNLFTYSVADALISQIEIGKRVLVQ
                     FGSQKIYAAIVLETSVAKPEDYEVKPILSVIDEIPVVTEKQVALWQWVHSYYLCFWGD
                     VMQAALPAELRLSSETKIVLNTEKETGSEYLTDDEFLVLEALHSVKELSLKEISRILD
                     RKHVFPVVKTLLQKKLVLSVENIGEDFKMPMANFIRRTAQTNDQDFLKKQFDHLEKKA
                     PRQLDVLMQLMQLEKNYNEGKVPQKVLLHKSGATAAALKQLVRKGLIEILIERATGSK
                     SFFNKDFYELSEAQSTALKEITKSFTEKPVTLLHGVTSSGKTELYIRLIDECLKNGKQ
                     ALYLLPEIALTTQIITRLEKHFGNKLMVYHSRFSDNERASVYTRMIENGFTNSEPFVV
                     IGARSSVFLPFHKLGLVIVDEEHESSYKQFDPSPRYHARDTAIVLAKLFEANVLLGSA
                     TPSIETYNNAITGKYGLVELFARHADIAPPQIQLVNIKELTFKRQMKSHFSPMLVNAI
                     KEELDKGKQIILFQNRRGFAPILQCHQCNWVPHCQHCDVALTYYKKADLLRCHYCGFT
                     QQPPSKCKSCGETDIRQYGFGTEKIEDELQIFFPEHNIARLDLDSTRGKHAYSEIISR
                     FETGETDILVGTQMVTKGLDFDNVSLVGILNADNLINFPDFRAYERSFQMLMQVSGRA
                     GRKKEQGKVIIQSYNPGHSILEFVVKNDFKSFIDFELSERKKFNYPPFSRLLEITVKG
                     RDESKTAQAVNLLTTELKKHIPSILGPVTPPISRIKNFYIRTLLIKFPRQAKLNDTRQ
                     MMLSAIDRYKINPVSKNIVLQIDVDPI"
     gene            5903..7606
                     /locus_tag="PKU82_06335"
     CDS             5903..7606
                     /locus_tag="PKU82_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ82529.1"
                     /translation="MNKNKIIILSTCMAVALIGIIAMQVNWILHDYHLKEQQFNQRVN
                     DALTSVAIKLEKKEAFNIFSNSFVTMNNDSIFSLFRERKMLDQEWEGLDSTTEDSIAN
                     KLSPENSIRPTPPIEPPLPPDFYNQEDYKNDFIKMEIKYSGGRKITIRHNRQEITLDD
                     SSKSNTHNAVKFLSLKADVLEKQRAKIEAKINNWNKVMQNLAFEFITDEKTIEDRIKP
                     FQLDSILKTELSNRGITTPYQFGVINVGENKIMSTNAPENKIALLKSRYKTDLFPNDL
                     LAKPILLSLNFPQGFSYVLSTMWVMLISSSLFILVMLFGFSYTIYTIFRQKKLSDIKN
                     DFINNMTHEFKTPIATISLATDAVNSPAISKNTESVHRYMNIIKEENQRMHKHVENIL
                     QLALFDRKHINLNLVEFDIHEMIQKAIEQIKLQVEVKGGKIETNLNAAQHQMLGDYDF
                     IFNSIINLLDNAIKYTPENPIISINTENIQNNIRILISDNGIGMSREVQKNIFEKFYR
                     AGTGNIHDVKGYGIGLSIVKAIALAHHGEIKVLKSIKDVGTTMEFLLPLNLRKAKRKH
                     N"
     gene            7635..8342
                     /locus_tag="PKU82_06340"
     CDS             7635..8342
                     /locus_tag="PKU82_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82530.1"
                     /translation="MSILTGKTKIMIAEDDTNFGLVLKDYLSLHEFDVHLYRDGRQAL
                     EKFEKGKFNLCILDVMMPVMDGFALAENIKAKDSNVPIIFLTAKSMKVDMIRGFKIGA
                     DDYITKPFDSEILLFKIKALLNRSETIVKAVNEQVEFVIGGFKFNSRLRTIQGFGKEE
                     KLSPKEAALLALLCVYLNDILPREVALRKIWNDDNYFTARSMDVFITKIRKYLKDDQN
                     IELLNVHGNGYRLVVKD"
     gene            8472..10409
                     /locus_tag="PKU82_06345"
     CDS             8472..10409
                     /locus_tag="PKU82_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82531.1"
                     /translation="MALLLLYPQFASADDLNKKINLYLRDTHSAQAADYVTSIIHHYL
                     VDDEVSDSAIMWSDTLRNLGEAKGDLELVAKSDVLTGKYYLRIANDAKALDYFYRALK
                     YYKSVDNLDGEADIYLQIGLVYYLQGQFPDALPNIKLATELYQKTSNDSSLSTTYYLT
                     GLSYLELKNYKQAEIFLQQSLKIGKLLSNNQREYECYVALARLYTNQKNFVAAEQLFD
                     KVMTKWDSIIDPYGYCMALIPYSKLLIETNKKEAAFINLNRALSIAEKFNFIRFKVDI
                     YKLLAIIYSDEKNFESANYYIIQHYQLRDSIYNSENQRAINTLKAKIEAEKKQAQLDS
                     LFQKEKADQLQKWLLALTIFMLLGLSFGLYRRYNYKQKREAELRIANGELSEALNSLR
                     QVQQQLIHNEKMASLGRMSSGIAHELRNPLNFVINFTKISSELIADIDKKIQNNHDEN
                     FEQLKNNLAMIYQHSLRADKIILNFSNHAKPKSNSRTIEDLNKVVSEFTSVAYHSFIS
                     HYPDFHCHIHYDFDQSLKGIVLNLQEITTVLLNLLNNAFDAMNEKLMVDKTYKPIMKI
                     QTRQIQNSVRLSISDNGPGIDEENLPRVYEPFFTTKTLGKGTGLGLSLSYDIIKSHNG
                     DMDVKSTPHLETEFSFTLPYN"
     gene            10438..10917
                     /locus_tag="PKU82_06350"
     CDS             10438..10917
                     /locus_tag="PKU82_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82532.1"
                     /translation="MKVSGYILLASLAINAVFAFNKFFVSNAVANNTSVGIDAPPCAC
                     DDKERYDKNEKDGGGEWISTNNGKAASDKFKATYKTVYKGAFFSKRALDEIFCKDNRA
                     NGIVCYFGQTGEKDSTVTIMIEGTHSETTLIRSGDQNAPVAFVATVKCPVICSAVGQ"
     gene            complement(10918..11427)
                     /locus_tag="PKU82_06355"
     CDS             complement(10918..11427)
                     /locus_tag="PKU82_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82533.1"
                     /translation="MKKISFIIMVCFFWSCTCSVVRDEPTENIQNGNSLKSNSDSVPE
                     YIKVDASAKINESEWKPVTGVVNVSINPDGKKNYAIMLTGDGITVALTYNGKEQTGKY
                     KTGEGFNASVLDAKSAVHNCNEGYIQFSEFDTTKKVMSGKFGITCMGNKASGNGYNIQ
                     EASFNNLKW"
     gene            complement(11477..12121)
                     /locus_tag="PKU82_06360"
     CDS             complement(11477..12121)
                     /locus_tag="PKU82_06360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOZ82534.1"
                     /translation="MHFISEELEDYCSQKTSKGSDLLQQIDRETNAKVLNPRMLSGHL
                     QGRLLSMISKMIKPTRILEIGTYTGYSALCLAEGLAEGGKITTIEKNAELESMIQKNL
                     SQSEYSDKIELHIGNALNIIPELKEQYQLVFIDADKENYPLYFKMIWDKLSSGGFVLA
                     DNVLWSGKVIEPTITGDIDTERIKMFNENVVNMPDVECVLLPVRDGLMLVRKNK"
     gene            12196..12588
                     /locus_tag="PKU82_06365"
     CDS             12196..12588
                     /locus_tag="PKU82_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82535.1"
                     /translation="MFRKGIRFILSFVLIFSVLASASGVTVFKMVCSKKNKTMVSLEQ
                     FSHCSNKENKECSFKRDCCQFSKSVIDANQLSNHENKVQVSTPAIVELPQLLENTNIF
                     SVFLPKFSIQEQTIPSPPLLLLISKLSI"
     gene            12644..>14708
                     /locus_tag="PKU82_06370"
     CDS             12644..>14708
                     /locus_tag="PKU82_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ82536.1"
                     /translation="MKKTISFIILLVISLQSHAQIIIRGVVKSDNSETLPGVSVYIPI
                     TDQHTVTDIDGKFQIQYTGTTPLTIISQFVGFKTDTMTLNAIPDNDLKIRLKKSIDLK
                     EVEIKSRRESTSISTITPINTTTITQHELLKAACCNLSESFETNPTIDVNYSDAVTGA
                     KQIQMLGLDGIYSQIQFQNLPLIFGLSASHGLAFTPGPWIESIQINKGIGSVINGFEA
                     MTGQINIELKKPQNADRFFINGYLNNEGREELNLQLAQRFKNHISTVFLAHGSMNHLK
                     VDRDKDGFLDQPLTKQVNAMNVWHITVPNKLEGEIGWRALYENRLGGQKGFNYDDDFG
                     KSTLYGVGIENKMFEVYTKTGTINPAKPYRSIALLTLSRLHLQDNYYGNKTYLGDQRS
                     FNANTIYQTIIHDTRHYIRSGLSFRYNHYSEKYNSVGTYTNEIIPGAYAEYSFNNDLN
                     WAVVAGLRADIHNTYGFFLTPRLHIKYNIDQLTAIRLSAGKGWRTARVYAENTSVFTS
                     SRALFIDDLKAEEAWNTGINFTKKMQWFGHDASFNADYYYTWFKNQVVIDMENIHQLR
                     FYNLDGKSFSHYLQSELNIEPISNLLLRFGYKYNLVKQTFSGELKSKPFTPRDKALMN
                     AEYKIKKGKYSIDATLKYTGKGRIPGGGININGYELKSNSESFMTLNSQATWRTKHAD
                     FYLGCE"
BASE COUNT         5205 a         2608 c         2465 g         4430 t
ORIGIN      
        1 aattaataca cagtaaaaaa cattacacta ttcaataaat cggaaaaatt gtcgtcagtt
       61 actgcattat atagacaatc tgacttacaa aatcactcaa ttatgccgat attgtcataa
      121 aaaaggtaat ggaattgaat ttgatgtatg ctgcatacat taacaattaa aacaaaaaca
      181 aaatgacact cattaaattt aaaaacgaac cggcatcaag aatgatggac cgctcaccaa
      241 tgttcagtga tttgttcaac gacttctttg atggtgtggt tagcaacaac atcactaaat
      301 cattcagccc ttctgtaaac gtaaaggaat ctgatcatga atttaagctt gaacttgcag
      361 ctcccggatt gagcaaggat gatttcaaga ttaacattga aaatgatatg ttgaacatca
      421 gtgcagagaa gaaagaagaa caagcaacac aaaacgagaa gtacacacac aaagaatttt
      481 cgtactcaag ctttaagcgc actttcacac tgcctgaaac tgctgataga gaaaaaattt
      541 ccgcagctta tgacaacggt gtgatgacct tagtaattcc taaaaaggac gaagcaaggc
      601 caaagccgtt gcgcgaaata aaaatttcat aatttaattt ggtttatttt agtggtgaat
      661 cctcttgtaa gaaattgcaa gaggatttta ttttaaacca tcagtgaaag accaactccg
      721 gcaagtatta ctgccatttt aataaaatta aaacgatggt tactatcact ttcgaacaaa
      781 atggttgtgc tgatatgcag aaaaatccca attacaattg ccataatttt actaaaccac
      841 tcagtcatca gcgtatcccc aagataaata ccaaagaatg cacctacagg tgacattgcg
      901 gcaaatacga ctaatgaaaa aacagaaact gtcttagaaa cacctgactg cataagcata
      961 ctcatcagtg caaatgcaac cggaatatgg tgcatagcaa ttcccatcat gagcgactgg
     1021 cgctgttgat tatcagccat tggtacacct tcaaaaaatg catgaataca taaactaatc
     1081 atcattgata ccggaaacac tgcatgttgt ttatgctcat gtaaatgaat atgcccatgc
     1141 tcaatcccct ttgaaaaata ttcaagaata atctgaaata aaaaacctgt cattacataa
     1201 atgccaatgg atttatcgcc ctgactaaaa acttcaggaa ctaaatgtgt aatggtaatt
     1261 ccaaacaaat atgcacctga aaatgccaga gagagtttca gcaatgaatc gttgctttta
     1321 ttcaataaga aataaagcag tcctccacct gtaatcagca ggaataaaat aataaatgcc
     1381 gtcattgcgg tttttgagtt ataaaaatca ttcgttcact gctatcttct tcaaaaggtc
     1441 tcaaagaata atcgccaaag atatgtcttg ttaccaagcc tacccgttca aagtgtttaa
     1501 gaaatgtgtg ttttgaaata atctgcacag actcagtaaa cttaaaactc tttcctgaat
     1561 cactaaactg aatcgttttc tctatggtat tcccatcaga gtgcttattt atgctgaaat
     1621 caataccatc tacttgtttt atgtgttgtt gtgaagtgtt tttgataaag tcaggattaa
     1681 aaaaatcaag aataaaatat cccccaggtt tcaatgccaa tgaagctgtt cttaaagcta
     1741 aacgattgtc gtgatctgaa ttgaaatatc caaaactggt aaatagattc agtgcaacat
     1801 cataacaatt ggttctgaaa atttcacgca tatcatgtac atagaattca agactttgtc
     1861 tttcaaactg tttacaatat gcaatacttt gctccgacaa atcaataccg gtaacatgat
     1921 aacctttgtc ggcaaggaat aaactgtggc ggcctttacc acatgcaata tctaaaacac
     1981 ttgactttac cggtaaatgg atttgttcca aaagattatc cataaaatat tctgcttcct
     2041 gattatccct atccttgtac agaatatgat agtaaggcga atcgaaccac gactgaaacc
     2101 acttttgcat aaaatacctt tcttcagccg gcaaatttat cgctattttt gacaacctca
     2161 tttatttaat aatggcaaag atcagaaggc taagtgtaaa actcagccgt aaaaaaacta
     2221 cacatccagg tgcacaaccc ggaagttttt cgataccatc agatgcttta acacctaagg
     2281 cactttgttt tatctatgac aaagattctt ttcaggagat tgatgttact caattcgatc
     2341 aattaaaaac tgcttatcaa aatttaggtg aaaaaacact ttggctgcat gttgccggat
     2401 ttggagataa aaccttttat gaaaacctga ctgcactttt tcaattacat agattggaac
     2461 tggaagatgt ctttaatgta tatcaacgac caaaggttga tgaatatgaa ggtcacttat
     2521 tttttgtgag cagactgctt agcatgactg atgaaacact taataacgat cagttaagtt
     2581 taattctagg aaaaaatttt gtcataacca ttcaggaaaa atatcaggac ttttttgatc
     2641 caattcgact aagattgagg aacggaaaag gcaatatgcg aacatatcgt cccggttact
     2701 tggcctatgc attaatggat gcagttattg accattacta tcccgcactt gaaaaaattg
     2761 gacttaagtt agatgatttg gaagatgaat tactaagcaa tccttctcag gattcaatga
     2821 acagaatact tcaaataaaa agggaactga tagtattcag aagagctatt tggcctgaga
     2881 gagataaaat aaacgacatt ttacgttcat catttgacga agtgcctgaa gaaacaaaat
     2941 tatttttcag agattcttac gaccattgca ttcagatact tgatattgtt gagagctata
     3001 aagaggttac tgcaagttta atggatgtat atctctcaag cgttagtcat aaacttaatc
     3061 aggtgatgaa agtactcact atcatttcaa caatttttat cccattaaca tttattgttg
     3121 gcctgtatgg catgaacttt caacttgcaa atccagaaac aggagaacgt ctgccaatga
     3181 atatgcctga attgtattcc ccctatggtt atgtgggtgt cattgctgtg atggtgctta
     3241 tagttgttat tcaattgtac atttttcatc gtaagggttg gatacgtttc agagaatgac
     3301 agagaaaagc agcagtggat tttatattaa agtagtagtg ccactaccac taccgaattt
     3361 atttacctat tcagtagcag atgcattaat ttctcagata gaaattggga aaagagtatt
     3421 ggtacaattt ggaagtcaga aaatttatgc agctatagtt ttagaaactt ctgtggcaaa
     3481 acctgaagat tacgaagtca agcctatcct ttcagtaatt gatgaaatac cagttgttac
     3541 agagaagcag gttgcactgt ggcaatgggt tcacagttat tatttgtgtt tttggggcga
     3601 tgtcatgcaa gcagcactac cggcagaatt acgtttgagc agtgaaacaa aaattgtact
     3661 taataccgaa aaagaaaccg gaagtgaata tctgactgat gatgagtttt tggtattgga
     3721 agctttacat tctgtaaaag aattaagcct gaaagaaata tcacgtattc ttgaccgcaa
     3781 acatgtattt cctgttgtta aaacattact tcagaaaaaa cttgtattat ccgtagaaaa
     3841 cattggtgaa gattttaaaa tgccaatggc taactttatc aggaggacag cacaaactaa
     3901 tgatcaggat tttctgaaaa aacaatttga ccatctcgaa aaaaaagcac caagacagtt
     3961 agatgttttg atgcaattaa tgcaattaga aaaaaattat aatgaaggca aggttccaca
     4021 aaaagtgctt ctccataaat caggagcaac tgcagcagca ttaaaacaat tagtaagaaa
     4081 agggttgatt gaaattctta ttgaaagagc aacaggctca aagtcatttt tcaataaaga
     4141 tttctatgaa cttagcgaag cacaatcaac tgcattaaaa gaaatcacta aatcttttac
     4201 agaaaaacct gtaacactgc tgcatggagt aacctcatcg ggcaaaaccg agttgtacat
     4261 aagacttatt gacgaatgtc ttaaaaatgg caagcaagca ctttaccttc ttcctgaaat
     4321 tgctttgaca acgcaaatca ttacaagact tgaaaaacat ttcggcaata aactgatggt
     4381 ttaccattca cgattcagtg ataatgaaag ggcatctgtc tatacaagaa tgatagaaaa
     4441 tggctttaca aattcagagc catttgtagt aattggagca cgttcatctg tatttctacc
     4501 cttccataaa ttaggtcttg taattgtgga tgaagagcat gaatcatcct acaaacaatt
     4561 tgatccttca ccacgctatc atgcacgcga cacagcaatt gtattagcta aactttttga
     4621 ggccaatgta cttttaggca gcgcaacccc atcaattgaa acttacaaca atgccatcac
     4681 cggaaaatat ggcttggtag aactgtttgc aagacatgca gatattgcac caccacaaat
     4741 tcaattggtg aatattaaag aactcacttt taaacggcag atgaaatcac atttttcacc
     4801 aatgttggtt aatgccataa aagaagaatt agataaagga aaacaaatca ttctatttca
     4861 aaacagacgt ggctttgctc ctattctgca atgtcatcaa tgcaattggg ttccccattg
     4921 tcagcattgt gatgtagctt taacctatta taaaaaagcc gatctgttac gttgtcatta
     4981 ttgtggtttc acacaacaac cgccatctaa atgtaagtct tgtggagaaa cagatatccg
     5041 tcagtacgga tttggcactg agaaaattga agatgagttg cagatatttt ttccggaaca
     5101 taacattgca cgtttagacc ttgacagcac aagaggtaag catgcctaca gtgaaatcat
     5161 cagtcgcttt gaaaccggtg agacagatat tttagtagga acacaaatgg ttacaaaggg
     5221 attagacttc gataatgtta gtttggttgg tatcttaaat gcggacaacc ttataaactt
     5281 tccagatttc agagcgtatg agcggagttt tcaaatgttg atgcaggtaa gtggtcgtgc
     5341 cggaagaaaa aaagagcaag gaaaagtaat cattcaatct tataacccag ggcattctat
     5401 actcgaattt gtagttaaaa acgacttcaa atctttcatt gattttgagt tatctgaacg
     5461 caagaaattc aactacccac cgtttagcag acttttagaa attaccgtta agggtcgtga
     5521 tgaatcaaaa acagcacagg ccgtaaacct tttgactaca gaacttaaaa aacatatacc
     5581 atctatactt ggccctgtca cacctcctat ttcaagaata aaaaactttt atatcagaac
     5641 tttactgata aaatttccgc gtcaggctaa attaaatgac acaaggcaga tgatgctttc
     5701 cgcaatagat cgttataaga taaacccggt gtctaaaaat attgttttgc aaatagatgt
     5761 agatcctatt taaaatttat tcttttactg aaaaactaaa tctcaaaaca caggaaccac
     5821 tcaatagcca gaattagtta aggtgggtta aattaagtta aacaacttta agctgtgtta
     5881 aagaatttta aatttgcatg taatgaataa gaacaaaatc atcattctct ctacatgcat
     5941 ggctgttgct ctcattggta ttattgccat gcaggtgaat tggattttgc acgactatca
     6001 tcttaaagaa caacaattca atcagagggt aaacgatgct ttgacttcag tagcaattaa
     6061 actggaaaaa aaagaagcat ttaatatttt cagcaatagt tttgtaacca tgaataacga
     6121 ttcaatcttt tcgcttttca gagagcgtaa aatgctggat caagaatggg aaggtctgga
     6181 ttccacaaca gaagactcaa ttgcaaataa actttcaccg gaaaattcaa ttcgacccac
     6241 acctcctatt gaaccgccac tacctcccga cttttataat caggaagatt acaaaaatga
     6301 ttttattaaa atggaaataa aatatagtgg tggcagaaaa atcactataa gacataatcg
     6361 tcaggaaata acgttagatg attcttcaaa atccaatacg cataatgctg ttaaattttt
     6421 atcgcttaaa gcggatgttt tagaaaaaca acgtgcaaag attgaagcta aaatcaacaa
     6481 ttggaataaa gtgatgcaaa atctggcttt tgaatttatt acagatgaaa aaacaattga
     6541 agacagaatt aaaccctttc agcttgattc aattttaaaa acagaacttt caaaccgggg
     6601 aataacaact ccatatcaat ttggtgtcat caatgttggt gaaaataaaa tcatgtcaac
     6661 caatgcacct gaaaacaaaa ttgcactcct gaaaagtcgt tataaaacag atttattccc
     6721 taacgactta cttgccaaac ctattttact ttcgctaaat tttcctcaag gtttttcgta
     6781 tgtactttct actatgtggg tcatgcttat tagttcatca ttatttattt tagtaatgct
     6841 atttggattc agctatacaa tctatactat tttccgccag aaaaaactgt ctgatattaa
     6901 aaatgacttt atcaataaca tgactcatga attcaagaca cctattgcga caatctcatt
     6961 agctaccgat gctgtgaaca gtcctgctat ttcaaaaaat acggaaagtg tacatcgtta
     7021 catgaatatt attaaagaag aaaatcaaag aatgcacaaa catgtagaaa atattctgca
     7081 acttgcatta tttgaccgaa agcatatcaa tcttaatctg gtagaatttg atattcacga
     7141 aatgatacaa aaggccattg aacagataaa actgcaggta gaagtaaaag gaggcaaaat
     7201 cgaaaccaat ttaaatgcag cacaacatca aatgctaggc gactatgatt ttatttttaa
     7261 ttcaataatc aacctgcttg acaatgccat aaaatacacc cctgaaaatc caatcatctc
     7321 tatcaatacg gaaaatattc aaaataacat tcgcatactt atttctgata atggaatcgg
     7381 tatgagccgt gaggttcaga aaaatatttt tgaaaaattt tatcgggccg gaaccggtaa
     7441 catacatgat gttaaaggtt atggtatcgg gttgtcaatt gtaaaagcta ttgcattggc
     7501 acatcatgga gaaattaaag ttttaaaaag tataaaagac gtaggaacaa ctatggaatt
     7561 tctattacca ttaaatctgc gtaaagcaaa aagaaaacat aattaataaa tagaacacaa
     7621 caacaaaaca taacatgtca atactaactg gaaaaaccaa aattatgata gctgaagatg
     7681 acaccaactt cggcttggtt cttaaagact atctttcttt acacgaattt gatgttcacc
     7741 tgtatagaga cggcaggcaa gctcttgaaa aatttgaaaa aggcaaattc aatctatgca
     7801 ttcttgatgt aatgatgcct gtaatggatg gctttgcatt ggccgaaaat attaaagcca
     7861 aagacagtaa tgtgccaatc attttcttaa ctgccaagtc aatgaaagtg gacatgataa
     7921 gaggttttaa gattggtgcc gatgattaca ttacgaaacc tttcgactca gaaattctgt
     7981 tattcaaaat taaagcatta ctcaacagaa gtgagacaat cgtaaaagca gttaacgagc
     8041 aggtagaatt tgttatagga ggcttcaaat ttaacagcag actccgaaca attcagggat
     8101 ttggaaaaga agaaaaactt tctcctaaag aagcagcttt acttgcttta ttatgtgttt
     8161 atctaaatga catcctaccg cgtgaagtag ctttacgcaa gatatggaat gacgacaatt
     8221 actttacagc tcgaagtatg gacgtgttta ttaccaaaat caggaagtat ctgaaagacg
     8281 accaaaacat tgaattattg aatgttcatg gcaacggata tcgtcttgta gtaaaagatt
     8341 gacattttgt acaatttttg attttaatgt aaaataagta tttatctttg gggacaaccc
     8401 tgccaggtat gcctaagaag aaaaaatgca tttatgctat caatctgagt ataaaaactc
     8461 ttagattttt tttggcatta ttactgcttt atccacaatt tgcctcagca gacgatttaa
     8521 ataaaaaaat taatctgtat ttaagagata cacatagcgc tcaagctgca gactatgtaa
     8581 cttcaattat tcaccattat ttagttgacg atgaggtttc tgattctgca attatgtggt
     8641 ctgacacgtt gcgcaatctt ggtgaagcta aaggcgatct tgagttggta gctaaaagcg
     8701 atgtgctgac cggaaaatat tatctcagaa tcgcgaacga tgctaaagca ttagattatt
     8761 tttacagagc cctgaaatac tacaaaagtg ttgacaatct tgatggtgaa gccgacatct
     8821 atctgcaaat cggacttgtt tactatctgc aaggtcagtt tcctgatgca ttaccaaaca
     8881 ttaaactagc aacagaacta tatcagaaga catcgaatga tagttccctt tctactacat
     8941 actatctcac aggcttaagt tatcttgaat taaaaaatta taaacaagca gaaatttttc
     9001 ttcaacaatc attaaaaata ggtaaacttt tatcaaacaa tcagagagag tatgaatgtt
     9061 atgtggcatt agctcgtctt tacaccaatc aaaagaactt tgtagcagct gaacagttgt
     9121 ttgataaggt aatgacaaaa tgggattcaa ttattgaccc ctatggatat tgtatggcac
     9181 taatcccata tagtaaatta ctgattgaaa caaataaaaa agaagctgct tttataaacc
     9241 tgaatcgtgc attgtcaatt gctgaaaaat tcaattttat aagatttaag gtagatattt
     9301 ataagctact tgcaataatt tattctgatg agaaaaattt tgaatctgca aattattata
     9361 tcatacaaca ttatcaactt cgtgattcaa tttataattc tgaaaatcaa cgtgcaatca
     9421 ataccttaaa agcaaaaatt gaagcagaaa aaaaacaggc acaattggat agtttatttc
     9481 aaaaagaaaa agcggatcaa ctgcaaaagt ggctgctggc attaactatt tttatgttat
     9541 tggggctttc attcggattg tataggcgat ataattacaa acaaaaaagg gaggcggaat
     9601 tacgaattgc aaatggagag ttgtcagaag cattaaatag tttaagacag gtacagcaac
     9661 aactcataca caatgagaaa atggcatcgt tagggcgtat gtcaagtggc attgcacatg
     9721 aactacgcaa tccattaaat tttgtcatta atttcactaa aatttcgagt gaattaattg
     9781 cagatattga caaaaaaatt caaaacaacc atgatgaaaa ttttgagcag ttaaagaata
     9841 atcttgcaat gatatatcag cactctttac gtgcagataa aataattctc aacttctcaa
     9901 atcatgcaaa gccaaaaagc aatagcagaa ctattgaaga ccttaacaaa gtagtcagtg
     9961 aatttaccag tgttgcatac cattctttca tttcacatta ccctgacttc cattgtcaca
    10021 ttcactacga ctttgatcag tcgcttaaag gtattgtatt aaatcttcag gaaatcacaa
    10081 cagtattact gaacttacta aacaatgcct ttgatgctat gaatgaaaaa ttaatggttg
    10141 ataagacata caaaccaata atgaagattc aaacacgtca aatacaaaac tcagtaaggc
    10201 tttccatttc agacaatggt ccgggtattg atgaagaaaa tttacctcgt gtttacgaac
    10261 ccttctttac tacaaaaact cttggtaaag gaaccggctt aggcctgagc ctatcatacg
    10321 atataattaa atcgcataat ggagacatgg atgtaaaatc aacaccacat ttggaaacag
    10381 aattctcatt tacattacca tataattaat tcataaataa ctttaaaatc aaaaattatg
    10441 aaagtatcag gttacatttt gctggcatcg ctggcaatta atgcagtatt tgcattcaac
    10501 aaattctttg taagcaatgc tgtcgcaaac aacacatcag taggcataga tgcaccgcct
    10561 tgtgcatgtg atgacaaaga acgttatgac aaaaacgaaa aagatggcgg tggagaatgg
    10621 atttcaacaa ataatgggaa ggctgcatct gataaattta aagcaaccta taaaacagtt
    10681 tacaaaggcg cctttttcag caaacgtgca ttggatgaaa ttttttgtaa agacaacaga
    10741 gcaaatggca ttgtatgtta ctttggtcaa accggagaaa aagattcaac agtaaccata
    10801 atgattgaag gcacacattc cgaaaccacc ttaatcagaa gtggtgatca aaatgcacct
    10861 gtagcttttg ttgcgacagt gaaatgtccg gttatctgtt ctgcagttgg gcaatagtca
    10921 ccacttaaga ttattaaacg aggcttcctg aatgttatat ccatttccgg aggccttgtt
    10981 tcccatacat gttattccga atttacctga cattactttt tttgtggtat caaattccga
    11041 aaactgaata tagccttcat tacaattatg tactgcagac tttgcatcaa gtaccgatgc
    11101 attaaacccc tcacctgttt tatactttcc cgtctgttct tttccattat aagttaatgc
    11161 aactgtaatt ccatcaccag tcagcatgat ggcataattc ttcttgccat cagggtttat
    11221 agacacatta accacccctg ttacaggttt ccattcagac tcattaattt ttgctgatgc
    11281 atcaacttta atatattcag gtactgaatc agaattagat tttaaactat taccattctg
    11341 aatattttca gttggctcat cgcgaacaac agagcatgtg cagctccaga aaaagcaaac
    11401 cataataata aaacttatct ttttcatttt gtatattttc atattgaaca aatgcgagaa
    11461 tttctaatta aactttctac ttattttttc taactagcat taatccatct cttacaggaa
    11521 gcaaaacaca ttcaacatcg ggcatattca ccacattttc gttaaacatt tttattcttt
    11581 ctgtatcaat atcccctgta attgttggtt caattacttt tccactccag agtacattat
    11641 ctgccaacac aaatccacca gatgatagtt tatcccaaat cattttaaag tacaaaggat
    11701 aattttcttt gtctgcatca ataaaaacta attgatactg ctcctttaac tccggaataa
    11761 tatttaatgc atttccaata tgtaattcga ttttatctga atattcactt tggctcaaat
    11821 tcttctgaat catactttca agctcagcat ttttctcaat cgtagtaatt ttaccgccct
    11881 cagctaatcc ttctgccaaa cataatgcag agtatccggt gtaagtacca atttccaaaa
    11941 ttcgggttgg ttttatcatt ttactgatca ttgacaacaa acgaccttgc aaatgtcctg
    12001 aaagcattcg gggattaaga actttggcat tagtctctct gtctatttgt tgtaataaat
    12061 cactgccttt tgaggttttt tggctacaat aatcttctaa ttcttcactg ataaagtgca
    12121 tggtatattt taatgcgaaa gtaatattta cctcaattca ttgccatttc agaaagttgt
    12181 tactattttt gtgtcatgtt tagaaaaggc atacgcttca ttctttcatt tgttttaatt
    12241 ttttcggtgc tggcatcagc ttcgggtgta actgtattta aaatggtttg ttccaaaaag
    12301 aacaaaacta tggtatcact tgagcaattc agccactgtt cgaacaaaga aaataaagag
    12361 tgtagtttta aaagagattg ctgtcagttt tcaaaatcag tgattgatgc caaccaactt
    12421 tctaatcatg aaaataaagt acaggtttca acacctgcca ttgttgaatt gcctcaactc
    12481 ttagagaaca caaacatatt ttctgtgttc ctgcctaagt tttcaattca ggaacaaaca
    12541 attccctccc cccccttact cctgctgatt tcgaaattat ctatttgatt caggctattt
    12601 ttatttagct tatttatcaa ataataattt taatcagatt tcaatgaaaa aaactatatc
    12661 atttattata ttacttgtaa tatcattaca aagtcatgcc caaataatca tcagaggcgt
    12721 ggtaaaatca gacaacagtg aaacactacc cggtgttagt gtatatattc caataaccga
    12781 tcaacataca gtaacagata ttgacggaaa atttcaaata cagtacacag gcacaacacc
    12841 acttaccatc atcagtcagt ttgtaggttt taaaaccgat acaatgacat taaatgcaat
    12901 acctgataac gatttaaaaa ttcgtctgaa aaagtctatt gacttaaaag aagtggaaat
    12961 aaaatcgcgc agagaaagta catcaatttc aaccatcaca cctataaata cgacaacaat
    13021 cacacaacat gaattgctga aagctgcatg ttgtaattta agtgagagtt tcgaaaccaa
    13081 tccaacaatt gatgtcaact acagcgatgc agtcacaggt gccaaacaaa ttcaaatgct
    13141 tgggcttgat ggtatctatt cgcaaataca gtttcagaac ttacctttga tttttggttt
    13201 gtctgcatca catggcttgg cttttactcc cggcccatgg attgaatcaa tacaaatcaa
    13261 taaaggaatt ggctcggtaa taaatggttt tgaagcaatg acaggccaaa taaatataga
    13321 gctgaaaaaa ccacaaaatg ccgatcgctt ttttattaat ggttatctga ataatgaagg
    13381 tcgtgaagaa cttaacctgc aacttgcaca aagatttaaa aatcatatca gcacggtttt
    13441 tcttgcacat ggcagcatga atcatcttaa agtggacaga gataaagacg gttttttaga
    13501 tcagccttta acaaaacagg tcaacgctat gaacgtttgg cacattactg ttcccaataa
    13561 actagaagga gaaattggct ggcgtgcctt atatgaaaac cgtttaggtg gacaaaaagg
    13621 atttaattat gacgatgatt tcggtaagtc aactttatat ggtgtaggta ttgaaaacaa
    13681 aatgtttgaa gtttatacaa aaaccggaac aataaatcct gccaaaccat acagaagtat
    13741 tgcattatta acgctttcac gtttacactt gcaagataat tattacggca acaaaacata
    13801 tttaggcgat caacgtagtt ttaatgccaa caccatttat cagactatca ttcacgatac
    13861 aagacattat atccgctctg gtttgagttt tcgttataat cattattccg agaaatataa
    13921 ttcagtcggc acctatacca atgaaatcat tcccggtgct tatgcagagt attcattcaa
    13981 caacgattta aactgggctg tcgttgcagg attaagagcc gatatacaca atacttatgg
    14041 cttcttcctg actccacgtt tgcatataaa atacaatatt gatcaattaa ctgcaattcg
    14101 tttatctgcc ggtaagggct ggcgcactgc cagagtttat gcagagaaca cttctgtgtt
    14161 tacttcttca agagcattat ttattgatga tctaaaagct gaagaagcat ggaatacagg
    14221 aattaatttc acaaagaaaa tgcaatggtt tgggcacgat gcatcattca atgccgacta
    14281 ttattataca tggtttaaaa atcaggtagt aattgatatg gaaaatattc atcaacttcg
    14341 tttctataat ttagatggga aatctttctc acattatctg cagagcgaat taaatattga
    14401 gccaataagt aatctgctgt tgagatttgg ttataaatac aacttagtta agcaaacttt
    14461 ttcaggtgaa ttaaaaagca aaccatttac tcctcgtgac aaagcattaa tgaatgcaga
    14521 atataaaatt aaaaaaggga aatacagtat tgatgcaaca ttaaaataca ccggcaaagg
    14581 cagaattccg ggtggaggta tcaacatcaa tggttatgaa ttaaaatcaa attcggaaag
    14641 tttcatgaca ttgaattcgc aagcaacctg gagaaccaaa catgcagatt tttatttagg
    14701 ttgcgaga
//
LOCUS       DAOOOL010000060        14650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_349_length_14650_cov_79.994039, whole genome shotgun sequence.
ACCESSION   DAOOOL010000060 DAOOOL010000000
VERSION     DAOOOL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_14650_cov_79.994039"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKU82_06375"
     CDS             complement(<1..811)
                     /locus_tag="PKU82_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ82537.1"
                     /translation="MKRFIALTYALLCFTLAFAQKPTQTVRGTITDQQSKEPLIGASI
                     VWLDSSTFIGATADINGNFRLNNIPVGRQAFKISFLGYRENVITSIITSGKEVVLNIE
                     LEQSVVTAKAVTIVAEKQKDKPNNELTTVSARSFTIEETSRYAGSVGDPSRMAANYAG
                     VSGANDSRNDIIIRGNSPTGLLWRLNGVEIPNPNHFGSLGTTGGPISILNNNVLDKSD
                     FLTGAFPAEYGNVLAGVFDLQMRTGNNEKHEFLAQVGFNGFEAGAEGPISKK"
     gene            1009..1947
                     /locus_tag="PKU82_06380"
     CDS             1009..1947
                     /locus_tag="PKU82_06380"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOZ82538.1"
                     /translation="MKILPAIRLADVASLLNAKYIGDAGFQITGINEIHKVKKGDITF
                     VDHPKYYDKALNSAATTILINKEVSCPEGKALIISDDPFRDYNLLTKKYRPLTFSMQQ
                     VDQTAVIGEGTVLMQGSVVGAHSKVGKNCLIYPGVVIYDHCEIGDGVIIHANSVIGAD
                     AFYFKRRPEGFDKMHTCGRVIIGDYVEIGACCTVDKGVSGDTVIGSGTRMDNHVHVGH
                     DTVIGKNCLFAAQVGIAGVVTIEDDVILWGQVGVQKDLTIGKGAVVLGQSGVGKTLDA
                     GKTYFGSPTTDARLKMRELALMKRLPEVFEFLDNKE"
     gene            1950..3176
                     /locus_tag="PKU82_06385"
     CDS             1950..3176
                     /locus_tag="PKU82_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOZ82539.1"
                     /translation="MEETTDKLAADLQSKKVQLHWLLQITKAINYNLPAEQLYQIYEN
                     VLHDHLKAGKLSLFVNENGWKQVLSYGMKEELGRLQPELFLNEISFSKSKTVATPQWL
                     EKDESVIPVYHNEMLLAYCIVGAIDPSQSISIKEVVPFIHTITNIIVVAIENKRLNKE
                     AVAQAGFRKELELAAEMQEMLFPVGLCGNEYFEIATHYLPHQQVGGDYYDFISLNKHE
                     HIFCMADVSGKGMAAALLMSNFQANLHALIKNRPVLTNLVAELNDCVSKTAKGEKFIT
                     FFIGLINFKKGEINYVNAGHNPPVFCSKNKSELLERGTTGLGMFDELPFINEGHVKIN
                     SGDSVFCYTDGITELENLKGDFYGMEALKLFIEQNMNNSSLDEFHKKLIADLDHFREG
                     NSFSDDVTILSLRSLS"
     gene            complement(3159..4544)
                     /locus_tag="PKU82_06390"
     CDS             complement(3159..4544)
                     /locus_tag="PKU82_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ82540.1"
                     /translation="MAVFSLVNEFYWLLILPAGILIGYSALYHLDKLFLFTAFCTPLA
                     INLSKTDLGIGVSLPTEPILFGMMIIYLLSKFIDGRYEKSFIKHPITVLILIHLAWML
                     ITSFTSSMFLVSIKHFVARLWFVVIGFMLATSIFKDEKKMRQFIWFYAVGMAITIIYT
                     ITRHSINGFTQQSAHWVMTPFYNDHTEYAAVLALMLPPIFGLMLLKHKSFMYRFFAFL
                     ISGLVIVAIVLSYTRATWLSLAVALVSMLAYVFRVKTWAVVAIIISMVVGFQIFQSTV
                     LYKFGKTKEYSQNDYRGHLKSITNIKTDASNVERLNRWSCAWRMFLDKPVFGWGPGTY
                     QFQYSVYQLQFEKTYISTNLGDKGNAHSEYLGPLSEQGFMGTIIFVALAVVVLYKASR
                     VFLQTNSNNIKVLALSMQVGLITYLVHGFLNNFLDKDKASIPFWAFVAAITVMDYYHN
                     KKQLNPSTERA"
     gene            complement(4612..5583)
                     /locus_tag="PKU82_06395"
     CDS             complement(4612..5583)
                     /locus_tag="PKU82_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOZ82541.1"
                     /translation="MNNAPVYEENFASLSLIQLIKKWKKQLMIVAVISIVLSFIFTLP
                     FFIPPMFKSTAIVYPENLSPYSTESATEQLLQLLESDEIKMELIKDFKLYAHYEIDST
                     KKTALTTVFKQLAGNLSINKTDYESVKIEMMDTDPLQAKQMADSIIEKGNRLARKLYR
                     EKTMEAYVVAKTQFDMKENELDSADKVLTELRMKSGIIEFSQQTREISRAYYTSILNK
                     SNNVRLEEMFQKLQQYGSDYVTLNEKINYTRGVYNNYKQYVENAEKDLTKELSYSNVV
                     TPPMIPDKKAYPVRSLIMLMFTASVVFLSFVVIIIYENSQEKKPQTN"
     gene            complement(5634..7070)
                     /locus_tag="PKU82_06400"
     CDS             complement(5634..7070)
                     /locus_tag="PKU82_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ82542.1"
                     /translation="MQRKFITNLALLLLLNLLIKPFWIFGIDRTVQNMIGAEGYGKYY
                     ALLNFSFLLNILLDAGITNYNNRNISQHQHLVSKHFSGIIILRFLLAGAYIIISLILG
                     FAIGYSKEDIHLLILLLANQFFISFILYLRSNLSGLHLFKRDSIISVTDRFLMILICG
                     YLIWYNNGTYLNIENFVHAQSASYLITAVVAITFLHGRISFQKLNWQPAFYIAILKKS
                     LPFALLILLMTFYSRIDSIMLERLLPDGAFYAGVYAQAYRILDAVNMFGFLFASLLLP
                     MFSHMLKKRQDIQPLTNLAFKLLMIPALTLAALGLFFSNDIMNLLYHEHGDLSAPVFT
                     MLMFSFVCISTSYIFGTLLTANGSLRNLNLTALGGMILNISLNLFLIPQFKASGAALA
                     GLITQIIMALAQIILVAKILHWKPDWKLNGKLLLFFILIALVADLAVRYYPEIKTTIA
                     IIILAPILAFSLGLLRISELKSIMKERQ"
     gene            complement(7113..8885)
                     /locus_tag="PKU82_06405"
     CDS             complement(7113..8885)
                     /locus_tag="PKU82_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82543.1"
                     /translation="MKRVYIFFALFAFLTLLAYLWQQRTFGNEKYERIVDSDGKGYVA
                     YLPALFIYQDLSFGFYSKPQNDKIARYFNDRFLTKQKDKTILKTYVGVAALMAPFYLT
                     GCLTQIMIHGITNGYEPMVQFFISMAAIFYALAGLFLIYKTLHLLNFSKTISALTSTL
                     IFAATNLCYYTLFHPTMSHVYSFFLISAFVYLSVCLTKNTNKKHLLLSAVTLGLIVIV
                     RPLNIIIILFPLLLLGKQKLTDLLKTKTGLLIVSALFFLMIISIQPILNYIQCGLLFP
                     WSYGDEGFYFTNPSLLKILFSFRNGLFIYSPLLFLSIPGLWLLRDSSIKITAATSLLL
                     IIVWYLLAAWWNWSSEPAFGNRSFVDWYGLMSLPIAASIGYIWNKSKVFLSILCFGFT
                     GLSTIQTWQYLNDIIHPDGMNFEKYRYVFLKTSPEYVASVGNGNEEAYLPEQRLPFFT
                     TKLNPENKSSNNHTETIFEFNAQKNKGAQFSITFSKNQLLPMKAMVTLQRKELEFKAC
                     TQATVCFDYADSGGSVYYNENLRINDIPRKGINEWRTFTYQFIMPALKDSNSQLNFYI
                     NNPKGARFEIKNIELKLFNFSNKQ"
     gene            complement(8882..9592)
                     /locus_tag="PKU82_06410"
     CDS             complement(8882..9592)
                     /locus_tag="PKU82_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ82544.1"
                     /translation="MIQKLSIIIPVFNESETIISVLEKVSDVQLINNIAKQIIIVDDH
                     SSDNSLEAITQFKNKHPQADITIVKQQVNMGKGYAVRTGMSKATGDYIIIQDADLELN
                     PDDFNILLKTSLDQNAQVVYGSRFLKKQQSQVPLLTLMANRILSMVTSLLTGKKVTDM
                     ETCYKLIDRSLLQNIQLKENRFGFEPEITIKLLKNNNIHFVEVPISYQPRTKKMGKKI
                     GVKDGIRALYCLTRYRFS"
     gene            complement(9589..9984)
                     /locus_tag="PKU82_06415"
     CDS             complement(9589..9984)
                     /locus_tag="PKU82_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HOZ82545.1"
                     /translation="MQTLSETINKVKEFHATFGLQYFEKPQAAIDKNIIALRHKLMQE
                     ENDEYLEAAMNNDLEQIADALGDKLYILCGTIIAHGLQHKIADVFNEIHRSNMSKLDE
                     NGKPIYREDGKIMKGNNYFLPDIKAVLEQ"
     gene            complement(10069..10587)
                     /locus_tag="PKU82_06420"
     CDS             complement(10069..10587)
                     /locus_tag="PKU82_06420"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ82546.1"
                     /translation="MLQTDKLQLRACEPDDLPLMYQWENNSDYWLLSDTTKPFSNYTI
                     SEFIKQDQNDIYTTKQQRFVMELRNPVKPIGFIDLYQFEAVHRRAGVGILIGDLSEQK
                     KGYAAQSLECLKNYSHTVLNMHQLYCYIQSSNKKSLALFQQAGFKECGVLKDWLINKN
                     KPEDVVMFQFIF"
     gene            complement(10620..11222)
                     /locus_tag="PKU82_06425"
     CDS             complement(10620..11222)
                     /locus_tag="PKU82_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOZ82547.1"
                     /translation="MKRNIVIFLILLMTSTAHAQELWDDVAIDSLPVFHSLNAAMQNP
                     DSVFRLDLSKQKLKEIPAQVFELTQLRELNVSRNHLTELPEQVYKLNNLQRLYANNNK
                     LTALPQRIGTLKNLQTLTLDRNRIEALPPEIGMLHNLEVLSLWDNELSEIPDEIKSLS
                     SVLKKLELRGILFNEEQQKRIHDLLPYTTIYFSPACNCKQ"
     gene            complement(11242..12168)
                     /locus_tag="PKU82_06430"
     CDS             complement(11242..12168)
                     /locus_tag="PKU82_06430"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOZ82548.1"
                     /translation="MKIYNHIDHFEKVSNAVITLGTFDGVHLGHKVIIERLKKIAVDV
                     NGQVIVLTFFPHPRMVLFPDDHGIQLLNTIDERTILMEKAGIDHLIIHPFSKEFSRMS
                     STEFVRDILVNKLGVKKLIIGYDHHFGRNREGSFTDLEELAPVYNFSVERIHEQLVND
                     VAVSSTKIRNALLAGDVATAREFLGYDYELTGTVVHGKKLGHSLGFPTANLHIREDYK
                     LVPANGIYAARTLVGGQWRDGVMSIGNRPTFDNGARSIEVNLFDFNSDIYGAKITIRM
                     SDFIRSEKKFENSAALIEAMNEDKLAAIKLLS"
     gene            complement(12270..13451)
                     /locus_tag="PKU82_06435"
     CDS             complement(12270..13451)
                     /locus_tag="PKU82_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOZ82549.1"
                     /translation="MLLKRWIVLLSIIISHQSTAQMLDSVITGAGQINNYLPIISDKN
                     VAVVANQTSIVGQTHLVDLLLSKHVKVKKIFTPEHGFRGVAEAGENIESGKDKSTGLP
                     IISLYGNHKKPTTDDLKGIDIVLFDLQDVGTRFYTYSSTLQYVMEACAENKKKLIVLD
                     RPNPNGYYVDGPVLEPEFKSFVGLNPIPVVHGLTMGEYANMLNGEGWLANKEQCDVTV
                     ISVKGYTHETRYKLTVNPSLNLPNMTAVYLYPSLCFFEGTIVSIGRGTTYPFQVVGYP
                     GFTEGPFEFTPKKNPEMKLPPLYENQKCRGFDLRDFGNYYIPSLKKLYLFWIKNIYDN
                     YQQKDKFFNDYFDKLAGTDKLRKQIIAGLNEEQIRRTWEEGLKSYKVIRKKYLLYPDF
                     E"
     gene            13551..>14650
                     /locus_tag="PKU82_06440"
     CDS             13551..>14650
                     /locus_tag="PKU82_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ82550.1"
                     /translation="MSGTVLAIAVTSIALSVMVMLVAVAIVTGFKKEITSKVVGFGAH
                     IRIKHFDNNQSYEEIPVNIDQKFINKLKSDNRISSVNAYATKAGIIRTSEDILGVAVK
                     GLDSTFNSGFFNPNMIEGSFNLQIHSGIYPAIISKSVADKMKLKLNDSFVVYFIDEPP
                     RARKFKVNGVYSTGLEEFDNLYVFCPLSVIQKLNNWNPLQAGGYEVNLKQLSDLESVN
                     ESVYKEAGFELNAQSIKTIYPEIFHWLDLQDVNVIIILVLMILVAGINMISTLLIILL
                     DNTTTIGLLKAIGAGNDGIRKIFLMVSSGIVGIGLIAGNVAGLLLCWLQYQFHLVSLP
                     QESYYVPYVPINLSVTNILLINAGTLVACILV"
BASE COUNT         4716 a         2508 c         2732 g         4694 t
ORIGIN      
        1 gttttttact gataggccct tctgcacctg cttcaaatcc attaaagcca acctgtgcca
       61 gaaactcatg tttttcattg ttgccggttc gcatttgtag gtcgaaaaca cctgcaagaa
      121 cgtttccgta ttctgcagga aaggcacctg taagaaaatc agatttgtcc aaaacattat
      181 tattaagaat tgaaataggg ccacctgtag tacctaatga accaaaatgg ttaggattcg
      241 gaatttcaac accattgagt ctccataaca atcctgttgg tgaattacct cgaatgatga
      301 tgtcgtttct ggagtcgttg gcaccactta cacctgcata gtttgcagcc atacgcgatg
      361 gatcaccaac actgcctgca tagcgcgaag tttcttctat tgtaaatgat cttgcactca
      421 ctgtagtaag ttcattattg ggtttgtctt tttgtttttc agcaacaata gtaacagcct
      481 ttgcagtaac tacagactgc tccaattcaa tatttaaaac cacttctttg cccgatgtga
      541 tgatgctggt gattacattt tcacgatagc cgagaaagga gattttaaaa gcttgccttc
      601 caacaggaat gttattcagt ctgaaatttc catttatatc agcagtagca ccaatgaatg
      661 ttgaagagtc aagccaaact atcgaggcgc caattaaagg ctctttactc tgctgatcag
      721 ttattgtacc tctgactgtc tgtgtaggtt tttgggcaaa tgcaagtgta aaacaaagaa
      781 gtgcataggt gagagcaata aatcttttca taaatttttt agagctgaaa aattaatccg
      841 gttcaaatga ttgatagttg aagataggac gtacaaaaaa acatatttgt tacatgcctg
      901 aaaaaatatt tttgttaaac actgttattt tttttataaa ttgattgcgg taattcagat
      961 ggctaatgac aacacattcg ttacatttgc agtcaaacag tttgaaatat gaaaatatta
     1021 ccagcaatca ggcttgctga tgtagcttca ctcctgaatg caaaatatat tggcgatgcc
     1081 ggctttcaga ttacaggcat caatgaaata cataaagtta aaaaaggtga tatcacattt
     1141 gtagatcacc caaagtatta tgataaagca ctaaattctg cagccacaac tattctgata
     1201 aataaagaag tttcctgtcc tgaaggcaaa gcattaatta tttcagacga tccgttcaga
     1261 gattataatc tgttgacaaa aaaatacaga ccattaactt ttagtatgca acaggttgat
     1321 caaactgctg tgattggtga aggaactgtg ttgatgcagg gaagtgtagt tggcgctcat
     1381 agtaaagtgg gtaaaaattg tctgatatat cccggtgtag ttatttatga tcattgcgaa
     1441 ataggtgatg gtgtaatcat tcatgccaac agtgttattg gtgctgatgc tttttacttt
     1501 aaaagacgcc ccgaaggatt tgataaaatg catacttgtg gtcgtgtaat tattggtgat
     1561 tatgttgaga tcggagcatg ttgtacagtt gataaaggcg tgtcaggcga tactgttatt
     1621 ggcagtggaa caagaatgga caatcatgtg catgtaggcc atgatacggt gataggtaag
     1681 aactgtttgt ttgctgcaca ggtgggcata gccggtgttg tcactattga agatgatgtt
     1741 attttatggg gacaggttgg tgttcagaaa gatttgacta ttggcaaagg agcagtggtg
     1801 ctcggccagt caggagttgg taaaacatta gatgcaggta agacttactt cggcagtccc
     1861 acaaccgatg cccgtttaaa aatgcgtgaa cttgcattga tgaaacgatt gcctgaagtg
     1921 tttgagtttt tggataacaa agaatagtga tggaagaaac caccgataaa cttgctgccg
     1981 accttcagtc aaagaaagta cagttgcatt ggcttttgca gattacaaaa gcaattaatt
     2041 acaacctgcc tgctgaacag ttgtatcaga tttatgaaaa tgttttgcat gatcatttaa
     2101 aggctggaaa actttctttg tttgtcaatg agaatggttg gaagcaggtg ctgtcctatg
     2161 gcatgaaaga agaactcggt cgtttgcaac cggaattgtt tctcaatgaa atatcatttt
     2221 ctaaatcaaa gacagtagcc acaccacagt ggcttgaaaa agatgaatcg gttataccgg
     2281 tttatcacaa tgaaatgtta ttggcttatt gcattgttgg tgcaattgac ccgagtcagt
     2341 caatttcaat aaaagaagtt gttccattca ttcataccat tacaaatatt attgtggtag
     2401 caatagaaaa taaacgttta aataaagaag cggttgcgca ggcaggattt cgcaaagaac
     2461 ttgaactggc agcagaaatg caggagatgt tatttcccgt tggcttatgt ggaaatgaat
     2521 atttcgagat agcaacacat tatcttccac atcaacaggt gggaggtgat tattatgatt
     2581 ttatttctct caacaaacac gagcatattt tttgcatggc agatgtttca ggaaaaggaa
     2641 tggcagctgc attgctgatg tctaatttcc aggctaacct gcatgcatta ataaaaaaca
     2701 gaccagtact tacaaactta gttgctgaat taaatgactg tgtgagcaag actgcaaaag
     2761 gtgaaaagtt tataaccttt tttatcggac tgataaattt caaaaaaggt gaaattaatt
     2821 atgttaatgc cggtcataat cctcctgtat tttgtagtaa aaacaagtct gaacttttag
     2881 aaagaggcac aacaggttta ggcatgtttg atgagttgcc atttataaac gaaggtcatg
     2941 taaaaataaa tagtggtgat tctgtttttt gttataccga tggtattaca gagctcgaaa
     3001 accttaaggg tgatttttat ggtatggagg cgttaaaatt atttatagag cagaatatga
     3061 acaattcaag ccttgatgaa tttcataaaa aactgattgc agatttggat catttcagag
     3121 agggcaatag ttttagcgat gatgttacca tcttatcatt acgctcgctc agttgatggg
     3181 tttagttgtt ttttattgtg ataatagtcc attacggtaa tagcagctac aaatgcccaa
     3241 aatggaattg aggctttgtc cttatctaaa aaattgttta aaaatccatg aacaagatag
     3301 gtaattaaac caacctgcat ggataatgca agcaccttga tgttgttaga attcgtttgc
     3361 aaaaagactc ttgatgcttt gtaaagaacc acaacagcta atgcaacaaa aattatagtt
     3421 cccataaaac cttgttcgct taatgggcct agatattcgc tatgtgcatt tcctttatcg
     3481 ccaaggttgg tgctgatgta ggttttttca aactgtagtt gataaacaga atactgaaac
     3541 tgataggtgc ccggtcccca tccgaaaaca ggtttatcta agaacattcg ccaggcacaa
     3601 ctccaacggt taagacgctc cacatttgat gcatcagtct ttatattggt aatagacttt
     3661 aaatgcccgc ggtagtcatt ctgtgaatac tcctttgttt ttccgaattt gtataacact
     3721 gttgattgaa aaatctgaaa acccacaacc attgaaatta ttatagctac tacagcccat
     3781 gtctttactc tgaaaacata agcaagcata gagacaagag ccacagccag actgagccag
     3841 gtggcacgag tgtatgataa tactattgca acaattacca atcctgaaat cagaaatgca
     3901 aaaaagcggt acataaaaga tttatgtttc aaaagcatca acccaaaaat cggaggcagc
     3961 atcaaagcaa gtacagcagc atattccgta tggtcattat aaaaaggtgt catcacccag
     4021 tgtgcagatt gctgtgtaaa tccatttatg ctgtgacggg tgattgtata gataatggtt
     4081 attgccattc caacagcata gaaccatata aactgccgca ttttcttttc atccttaaag
     4141 atggatgttg caagcataaa gccaataaca acaaaccaca atcttgctac aaaatgtttt
     4201 atagaaacaa gaaacatgct gcttgtgaaa cttgtaatca gcatccatgc aagatgaatc
     4261 agaatgagaa cggtaattgg atgtttgata aaggacttct catacctgcc atcaataaat
     4321 tttgaaagaa gatagatgat catcatgcca aacaaaatag gctctgttgg cagactgaca
     4381 ccgattccta aatcagtttt tgaaaggtta atcgctaagg gagtgcagaa tgcagtaaaa
     4441 agaaataatt tgtcaagatg atagagtgca gagtacccga ttaaaatgcc cgcaggcaaa
     4501 atcaatagcc aataaaattc attcaccaaa ctgaatactg ccattagcat atatatggct
     4561 gtaaggccat aaaaccaccg aagcgaattt tgctgaatgg cttgcataaa attaatttgt
     4621 ttgaggtttc ttttcctgag aattttcgta gatgatgatt actacaaaag ataagaatac
     4681 cacagaggca gtaaacatca gcattattaa actgcgaaca ggataggctt tcttatccgg
     4741 aatcattggt ggcgttacca catttgaata tgaaagttct ttggttaaat ctttttcagc
     4801 attttctaca tactgcttgt agttgttata aactccacgg gtgtaattga ttttctcgtt
     4861 gagtgtcaca taatccgagc cgtattgttg caatttctga aacatttctt ccaaacgcac
     4921 attattgctt ttatttaaaa ttgaagtata atatgctctt gaaatttcgc gtgtctgttg
     4981 tgagaactca atgataccgg acttcattct taactctgtt aaaaccttat cggcagaatc
     5041 gagctcattt tctttcatat caaattgtgt tttggcaacc acgtaagctt ccatcgtttt
     5101 ttctctgtaa agttttcttg caagcctgtt acctttttct attatagaat cggccatttg
     5161 tttagcctgc aatggatcgg tatccatcat ttcaatttta actgattcgt agtcggtttt
     5221 attgatactc aaattgcctg caagttgttt aaaaacggta gttaaagcgg ttttcttggt
     5281 tgagtcaatt tcataatgtg catagagttt aaaatctttt atcaactcca tttttatttc
     5341 atcagactca agcaactgta ataactgttc tgtagcactt tcagttgaat aaggacttag
     5401 gttttccgga taaacaatag ccgttgactt aaacataggt ggaataaaaa acggaagcgt
     5461 aaaaataaat gacagtacaa tagaaattac tgcaacaatc attaattgct ttttccattt
     5521 tttaatcaat tgaatcagac ttagtgatgc gaagttttct tcgtaaacag gggcattatt
     5581 cattatgaag gaatttattt aagagccgca aatgtaaaca aattagcgtt tcgctattgt
     5641 cgctctttca ttattgattt tagctctgaa atgcgaagta gtcctaaaga aaatgcaagg
     5701 ataggtgcca gtataataat ggcaattgtt gtctttattt ccggataata tcgtactgct
     5761 aaatctgcta ctaaagcaat caatataaaa aataataata actttccgtt gagtttccaa
     5821 tccggtttcc aatgcaggat ttttgccacc agaattattt gtgccagtgc cataataatt
     5881 tgagtaatca agcctgctaa tgctgcaccg gatgctttga attgtggtat caaaaacaga
     5941 ttaagagaaa tgtttaatat cattccacct aatgcagtaa gattaaggtt tcgcaaactt
     6001 ccatttgcgg taagcaatgt tccaaatatg tatgatgttg aaatacaaac aaaactaaac
     6061 ataagcatag taaaaacagg agcagacaaa tcaccatgtt cgtgataaag taagttcata
     6121 atatcattgc tgaaaaacag tcctaatgca gccaatgtca atgcaggaat cattagaagt
     6181 ttaaatgcaa ggtttgtaag tggttgaata tcctgtcgtt tttttagcat gtgtgaaaac
     6241 atgggtaaaa gtaaggaggc aaataaaaaa ccaaacatgt taacagcatc taaaattctg
     6301 tatgcttgtg cataaactcc tgcataaaat gctccatcag gtaaaagtct ttccaacata
     6361 attgaatcta ttctgctata gaatgtcatc aataggatga gtagtgcaaa agggagtgat
     6421 tttttaagaa tagcaatata aaatgcaggt tgccagttta atttctgaaa actgattcgc
     6481 ccatgtaaaa aagtaatagc tactactgct gtgattaaat atgaagccga ttgagcatga
     6541 acaaaatttt cgatgtttaa ataagtgcca ttgttatacc aaatcagata accgcatata
     6601 agtatcatta aaaaacgatc ggttacggaa ataatactgt cacgcttaaa aagatgtaat
     6661 cctgacaagt ttgagcggag atatagtata aatgatataa aaaactgatt ggctaagagt
     6721 aatatcaata aatgaatatc ttcttttgaa taaccaatag caaagcctaa aattaaactg
     6781 attattatgt atgcaccggc caataaaaat cgcagaataa ttattcccga aaaatgttta
     6841 gagactaaat gctgatgctg cgaaatattt ctgttgttgt aattagtaat tcctgcatcg
     6901 agcagaatat ttaacagaaa agaaaaattt aataatgcat aatattttcc atagccttct
     6961 gcaccaatca tattctgtac agttcggtca atgccgaaaa tccagaatgg cttgatgagc
     7021 aaattcaaca gcagcagtaa ggcaaggttg gtgataaact ttcgttgcat cgaatgcaaa
     7081 aatagaataa ttaaaacggt ttgatgtact tactattgct tgtttgaaaa attgaataat
     7141 tttaactcta tgttcttgat ttcaaaacga gcacctttag gattattgat ataaaagttt
     7201 aattgtgaat tggagtcttt tagtgcaggc atgataaact gataagtaaa tgttcgccat
     7261 tcattgattc ctttacgcgg aatatcattg attctcaaat tttcattgta ataaacactc
     7321 ccaccagaat cagcataatc gaaacaaacg gtagcttgtg tacaagcttt aaactcaagt
     7381 tcttttctct gaagtgttac catagctttc atgggcaaaa gttgattttt tgaaaaagtt
     7441 attgaaaact gtgctccttt attcttctgt gcattgaatt caaagattgt ttcagtatga
     7501 ttgtttgatg atttgttttc aggatttaat tttgttgtaa aaaagggtag tcgttgctcc
     7561 ggtaaataag cttcctcatt tccatttcct actgaggcaa catattccgg ggaagtcttt
     7621 aaaaaaacat aacgatattt ttcgaaattc attccgtcag gatgaatgat gtcattgaga
     7681 tattgccagg tttgtattgt gctaagtcct gtaaacccaa aacagagtat tgataaaaag
     7741 actttacttt tattccatat atagccaata gatgcagcaa taggtaatga catcaagcca
     7801 taccagtcaa caaaacttct gttgccgaat gcaggctctg atgaccagtt ccaccatgca
     7861 gccaaaagat accaaactat aataagtaat agtgatgttg cagcagtgat tttaatggaa
     7921 ctatcacgta gcagccataa tcccggaatt gacagaaaca gtagtggcga atatataaat
     7981 agaccattcc tgaaactgaa aagtattttc aaaagtgaag gattggtaaa ataaaatcct
     8041 tcatctccat aactccaagg gaaaagcaat ccacattgta tataatttaa tataggctga
     8101 atggaaataa tcattaaaaa gaataatgca gatacgataa gcaatccggt ttttgttttt
     8161 agtaaatcag taagtttttg ttttcctaac agcaacaatg gaaaaaggat gattataata
     8221 tttaacggac gaacgataac aattagtcct aaagtgacag cagaaagtag aagatgtttt
     8281 ttatttgtat tttttgtaag gcatactgat agataaacaa atgcagagat taaaaagaaa
     8341 gaatacacat gcgacatggt agggtggaag agggtgtagt agcaaagatt tgtagctgca
     8401 aaaatcaatg tggaagtaag tgctgagata gttttgctaa aattcaataa atgcaatgtt
     8461 ttgtaaataa gaaataatcc tgccaaagca tagaaaatgg ctgccataga aataaagaat
     8521 tgaaccattg gctcataacc atttgttatt ccatgaatca ttatttgtgt cagacatccg
     8581 gttagataaa aaggtgccat taaagcagct acaccaacat aggtctttaa aatcgtcttg
     8641 tctttttgtt ttgttaaaaa tctatcattg aaatatcggg ctattttatc attctgtggc
     8701 ttagaataaa aaccaaaact taaatcctga taaataaata aagcgggtaa ataagcaaca
     8761 taacctttgc catcactatc aacaatgcgc tcatattttt cattgccaaa ggtgcgttgt
     8821 tgccagaggt atgcaagcag tgttaagaaa gcaaaaagtg caaaaaaaat gtaaactctt
     8881 ttcatgaaaa ccggtaacga gttaaacagt ataatgccct gataccatcc ttaacaccaa
     8941 ttttctttcc catttttttt gtgcgtggtt gataagaaat tggtacttca acaaaatgaa
     9001 tgttgttgtt tttgaggagc tttatcgtaa tctcaggctc aaatccaaaa cgattttctt
     9061 tgagttgtat gttttgcagt aatgatcggt caattagttt ataacatgtt tccatgtctg
     9121 taactttctt acctgtaagc aggcttgtta ccattgataa aatcctatta gccattaaag
     9181 tcaacaaagg cacttgtgat tgttgttttt ttaagaaccg tgagccatat actacttgtg
     9241 cattttgatc taaagaagtt ttcaatagaa tattaaaatc atcaggattt aattcaaggt
     9301 ctgcatcctg aatgataatg taatctcctg ttgctttact catacctgtc cgcacagcat
     9361 aaccttttcc catattgacc tgctgtttaa caattgtaat atcagcttgt ggatgtttat
     9421 ttttaaattg cgttatagct tctaaactat tatcacttga atggtcatca actataatta
     9481 tttgtttggc gatgttgttt attaattgaa catcagaaac tttttccaat acagaaatta
     9541 ttgtttcaga ttcattaaat acgggaatta taatgctcag cttctgaatc actgttccaa
     9601 tactgcttta atatcaggaa gaaaatagtt gttacctttc attattttac catcttctct
     9661 gtaaattggc tttccgtttt cgtcaagctt actcatattg ctgcgatgta tttcattaaa
     9721 tacatcagca attttatgtt gcaatccatg agcaatgatg gtgccgcata gaatgtacag
     9781 cttgtctccc agtgcatcag caatttgttc aagatcattg ttcatggcag cttcaagata
     9841 ttcatcattt tcctcctgca tcagcttatg acgaagagca ataatgtttt tatctattgc
     9901 agcttgtggc ttttcaaaat attgaagtcc gaaggttgca tgaaactcct ttaccttatt
     9961 gatggtttct gataatgttt gcattttgtt tgatttagta gcgtagcaaa actgaacaaa
    10021 aaagttcaat ttgcttgggt gtgacgaaca actgttaata aaaagtaatc agaatataaa
    10081 ttggaacatt actacgtctt caggcttatt tttattaatg agccaatctt tcaatactcc
    10141 acattcttta aatcctgctt gttgaaagag tgctaatgac tttttatttg atgactgaat
    10201 gtagcagtaa agctgatgca tattcaaaac tgtatgcgaa taatttttta aacattcaag
    10261 actttgtgca gcatatcctt ttttttgttc actcaggtcg ccaattaaaa ttcctacacc
    10321 tgcacgtctg tgaacggctt caaactgata gaggtcaata aatccaattg gttttacagg
    10381 gttacgtaat tccattacaa aacgctgctg ttttgtagta taaatatcgt tctgatcttg
    10441 cttgataaat tctgaaatgg tgtaattgga aaatggcttt gtagtgtcac taagcagcca
    10501 ataatcagag ttgttttccc actgatacat taatggcagg tcatcaggtt cgcaggcacg
    10561 caattgtaat ttatctgtct gtaacatata ggcaaaaata tataatcaga ctgacttgtt
    10621 tattgcttgc agttgcaggc aggcgaaaaa tagatggttg tataaggcaa caagtcgtgt
    10681 attctttttt gttgctcttc gttaaaaaga attcccctta attctaattt tttcaatacg
    10741 cttgaaagac tttttatttc atcaggtatt tcagacagtt cattatccca aagacttaaa
    10801 acttcaagat tatgcaacat tcctatttcc ggtggcaatg cttcaatgcg attgcggtca
    10861 agtgttaaag tctgaagatt ttttaaagtt ccgatgcgtt gtggcaatgc tgttagtttg
    10921 ttgttgtttg catacaaacg ttgcaggtta ttgagtttgt aaacttgctc cggcaattct
    10981 gttaaatgat ttctgctgac attcagttca cgcagttgtg taagttcaaa aacctgcgca
    11041 ggtatctctt tcaatttttg ttttgatagg tcaagcctga aaactgaatc cggattttgc
    11101 atagctgcat tcagtgaatg aaatacaggt aatgaatcta ttgcaacatc gtcccacaac
    11161 tcttgtgcat gtgctgtaga tgtcatgagc aaaatcagaa atatgacgat gttgcgtttc
    11221 atgacccgtt taacgtaaat tttacgataa aagtttaatt gcagccaatt tatcttcatt
    11281 catggcttct atcagggctg cactgttttc aaatttcttc tcactccgta taaaatccga
    11341 catacgaatg gtaatctttg caccgtaaat atcggaatta aaatcgaata aatttacctc
    11401 tatagaacgt gctccattgt cgaatgtagg tctgtttcca atactcatca caccatcacg
    11461 ccattgacca ccaaccaagg ttcgggctgc ataaatgcca tttgccggaa ccaatttgta
    11521 atcctcgcgt atatgtaggt tggccgttgg aaacccaagg ctgtgaccta attttttgcc
    11581 atgaacaaca gttcctgtta actcataatc atatcctaaa aactcacggg cagttgccac
    11641 atcgcctgcc agcaaggcgt ttctgatttt tgttgagctt actgcaacat cattgactaa
    11701 ttgctcatga attctttcta cagaaaaatt ataaaccggt gccaattctt ccaaatcagt
    11761 aaaagagcct tctctgttgc gtccaaaatg atggtcgtag ccgatgatca gtttttttac
    11821 accaagtttg ttgactaaaa tatcgcgaac aaattcagta gaactcattc ttgaaaattc
    11881 tttcgaaaaa gggtgaatga taagatgatc tattccagct ttctccatca gtatagttcg
    11941 ttcatcaatt gtgttgagca gttgaattcc gtggtcgtca ggaaataaaa ccattcgggg
    12001 gtgcggaaaa aatgtaagta ctataacctg cccgttaaca tctacggcaa tttttttcag
    12061 tctttcaata attactttat gcccaagatg aacaccatca aatgttccaa gtgttattac
    12121 ggcattgctg accttttcaa aatggtctat atggttatat attttcaaaa atactgtgga
    12181 taaagtgcaa aaatacaaaa gaatagccga gcgttaaaac catgactgat aaagccactt
    12241 gtttatttga aaaaaccaaa ttgtaatcgc tattcaaaat cgggatagag taaatatttt
    12301 tttcgaataa ctttataaga cttcaagcct tcttcccagg ttcgtcttat ctgttcttcg
    12361 ttcaggccgg caattatctg tttgcgcagc ttgtctgtgc cggcaagttt gtcgaagtaa
    12421 tcgttaaaga atttatcttt ttgctgatag ttgtcgtaaa tatttttaat ccaaaacaag
    12481 tatagttttt taagtgatgg gatgtaatag ttcccaaaat cacgcaggtc aaagcctctg
    12541 cacttttgat tttcgtagag cggaggaagt ttcatttccg gatttttttt aggtgtaaat
    12601 tcaaacgggc cctcagtaaa accggggtag ccaacaacct gaaatggata ggtagtaccg
    12661 cggccgatac tcacaattgt tccttcaaaa aaacacaagg acgggtacag ataaacggct
    12721 gtcatgtttg gtaaattgag tgaaggatta acagtcagtt tatatcttgt ttcatgcgta
    12781 tatcctttga cgctgataac agtaacatca cattgctctt tattggcaag ccaaccttca
    12841 ccattcagca tgtttgcgta ctcacccata gtgagtccgt gtacaacagg aatagggtta
    12901 aggccaacaa aagatttaaa ttcaggttca agcacagggc catcaacata atatccatta
    12961 ggattaggtc tgtctaaaac aatcagtttc tttttgtttt ctgcacaggc ctccataaca
    13021 tattgtaatg ttgaactgta ggtgtaaaaa cgtgtaccaa catcctgcaa gtcgaacagt
    13081 acaatatcaa taccttttaa atcatcagtt gttggctttt tatgattacc ataaagagaa
    13141 ataatgggca ggcctgtact tttatcttta ccggattcaa tgttttctcc cgcttcggca
    13201 acgcctctaa aaccatgctc aggagtgaaa attttcttga ctttaacatg cttggaaaga
    13261 agcaagtcaa ccaaatgtgt ttggccaact atacttgtct gattggcaac cacagcaaca
    13321 ttcttatcac tgatgatcgg caaataatta ttgatttgtc cggcaccggt aattacactg
    13381 tcaagcattt gagcagtgct ttgatggctg ataattatgg atagtaaaac tatccaacgc
    13441 ttcaaaagca taaatttgaa accgaaatat cagcaagaat aataaattag ttgagatact
    13501 cttacttcat agcaaagcgt tttttcacag gcagtgttaa caaccaacgt atgtcaggca
    13561 ctgttctggc aatagctgta accagcattg cactaagtgt aatggtgatg cttgttgctg
    13621 ttgcaatagt cacaggtttt aaaaaagaaa ttacctctaa ggttgttggc tttggcgcac
    13681 atatcagaat aaaacatttt gataacaatc aatcctacga ggagattcct gtaaatattg
    13741 atcagaagtt tataaacaag ctaaagagtg ataacagaat cagcagtgta aatgcctatg
    13801 ccaccaaggc cggaataatc cgcacttctg aagatatttt aggtgtagct gtaaaaggat
    13861 tagattcaac ttttaactca ggttttttta atccgaatat gattgaagga agtttcaatc
    13921 ttcagataca ttctggtatt tatcctgcta taatttcaaa aagtgtagca gacaaaatga
    13981 aattaaagct gaatgattct tttgtagttt attttattga tgagccgcca agagcccgca
    14041 agtttaaagt caatggagtt tattccacag ggcttgaaga gtttgataac ctctatgttt
    14101 tttgcccact ttctgtaatt cagaaattaa acaactggaa tccattgcag gccggaggct
    14161 atgaagtaaa tttaaagcaa ctcagcgacc ttgaatctgt caatgaaagt gtctataaag
    14221 aagcaggttt tgaattgaat gcgcaatcca tcaaaacaat ttatcctgaa atatttcatt
    14281 ggcttgactt gcaggatgtc aatgttatca tcattcttgt tttgatgata ttggttgcag
    14341 gaatcaatat gatttctaca ctcttgatta tcttattaga caatacaaca acaattggtt
    14401 tgttgaaagc cattggtgca ggaaatgacg gaataagaaa aatttttctg atggtttctt
    14461 caggaattgt tggcattgga ttgattgcag gaaatgttgc aggacttctt ttgtgctggt
    14521 tacaatacca atttcatttg gttagtctgc cacaggaatc atattatgtt ccttatgttc
    14581 caataaattt atcggttact aatattctgc tgattaatgc aggtacttta gttgcatgta
    14641 tattggtgat
//
LOCUS       DAOOOL010000061        14524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_355_length_14524_cov_71.228350, whole genome shotgun sequence.
ACCESSION   DAOOOL010000061 DAOOOL010000000
VERSION     DAOOOL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_14524_cov_71.228350"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKU82_06445"
     CDS             <1..773
                     /locus_tag="PKU82_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ82551.1"
                     /translation="LRFVTYYYFLFAAKPKKILINFYQRALKVNEVVAKKLCRKNFYY
                     LGQTLVDRTAFLLGKGNVFSHNFENEKFLVEMQEQRKGGILISAHVGNWETAGNLLKS
                     RVSSTIHVLMVDAEAKKLKQYLDNSTGGSQFSIIAVKDDLSHIVKVNNALAANELIAM
                     HGDRYLPGTKTIQLDFMGRKAHFPYGPFLLASRFNAPVTFVFALKANDFHYELSATKP
                     ITEKKSPEDIAALYVEELEKKVKKYPEQWFNYYDFYSE"
     gene            770..1186
                     /locus_tag="PKU82_06450"
     CDS             770..1186
                     /locus_tag="PKU82_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82552.1"
                     /translation="MIIEKVNIENYIPQRFPFVMIDNLIHANESGFLSSFLVKKENIF
                     SENGYLTEPALIENIAQTCAAGFGFIGQQKNEGPSIGYIGSISKLTVHQLPEYGTTIL
                     TTVEITNTLGNIFLIKGKNTCNEKLLVECEMKIALA"
     gene            1183..1611
                     /locus_tag="PKU82_06455"
     CDS             1183..1611
                     /locus_tag="PKU82_06455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOZ82553.1"
                     /translation="MKQLLQVEIPIKVRFSDTDAMGVVWHGNYLRFFEDAREAFGIKY
                     GLTYLDVYHRGFFTPIVKSSVEHKSPINYGEEVVAIAKFHPTPSAKIMFDFDILNKTT
                     GKLSAKGHTTQVFLSVVEREMELVKPPFYIEWENSIGIKH"
     gene            1615..2691
                     /locus_tag="PKU82_06460"
     CDS             1615..2691
                     /locus_tag="PKU82_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOZ82554.1"
                     /translation="MIYIGSETILSPNGNVVDCFKKIAEKKSGVSKIPGNGFKGESFY
                     CSMYLENRPDFNTLAAQTIEQLLQKSKANLLTEESTLLIISTTKGDLSKGFENAIISP
                     AKFLYEKLKLKHFPLIISNACASGVMAINAAAAFIENKQYQNIIVLGIDMITDFIVYG
                     FQSLYAISDEHCKPFDNDRKGINLGEGAGAVLLSASKDVFTSTPVCFLGGSSSNDANH
                     ISGPSRTGEGLFRSINKTFNSAGIKTEDIDFISAHGTATVFNDEMEAIAFNRLNMQHI
                     PVNSFKGFTGHTLGAAGVIETALCITCMHENKLLPSAGYYNKGTTVELNLIKDAASAT
                     INTVLKTASGFGGCNASLLLQKIK"
     gene            2688..3299
                     /locus_tag="PKU82_06465"
     CDS             2688..3299
                     /locus_tag="PKU82_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82555.1"
                     /translation="MKITGCCRIHQNEVLVNGKKYFTSDKNQHEELSMQLYRFLKTEY
                     PKFYKMDLLSKMGWLATEIVCQTIPTISKFADDEIALLFANSHSCSESDERFNHSFKV
                     ENSPSPSQFVYTLPNILLGELAIRHKWYGENLFTILPEFSAEHFSSHAGIFIPQKAKA
                     LFCGWINLFPVTDVFVFFAEANSETSPGILDSKEIEHWYKIIK"
     gene            3301..3558
                     /locus_tag="PKU82_06470"
     CDS             3301..3558
                     /locus_tag="PKU82_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOZ82556.1"
                     /translation="MDNLKEELKKHLITQLNLEDKTPADISDEAPLFGDSGLGLDSID
                     ALELIVLLDKHYHIKLTNPEEGKSVFYSISTMAEYIEKNKK"
     gene            3565..4743
                     /locus_tag="PKU82_06475"
     CDS             3565..4743
                     /locus_tag="PKU82_06475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ82557.1"
                     /translation="MKVYVTGIGVVSAIGLTVEENYHSLLNKKSGITAFKFPEEQKSI
                     QAGSIAKSNAELKQMLSITNGNFSRTSLLGIIAAEQAMGKNFHNKKLRTGFISSTSVG
                     GMDKTEKYYRQVLKNLPVDFNLTRTHDSGNTSENIAAYLGFSGYINTLSTACSSAANA
                     IMLAARMIEQGKMDRVLVGGTDPITDFTIKGFQSLMIYDDEWCKPFDHNRNGLNLGEG
                     AGFLLLESDHSINISGNEPICSLTGWHNAADAYHQTASSPDGKGATIAMKNALKKAGL
                     GPKEISYINAHGTGTKNNDLSESIAIKNVFGESIPPFSSTKAYTGHTLAAAGAIEAVY
                     SVLAIKNQIHFAGLNYNDPLEETLMHPVTEPTKAVTKHVMSNSFGFGGNNSSLIFSGI
                     "
     gene            4746..5711
                     /locus_tag="PKU82_06480"
     CDS             4746..5711
                     /locus_tag="PKU82_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOZ82558.1"
                     /translation="MYCIQSASTISHQPSFASKGFSSNLSKEIESLIKPDYKEYVPPA
                     LIRRMSEILRMSVACSNLALKEAGIEQPDAIITGTGLGCLYDTEKFLNNVITIEGGLL
                     PPTSFIQSTHNTIAGQISLVLGNHQYNMTHTQNSLSFEHALLDTMLMLDEKNGNILIG
                     SADEDIEALHPIAAHFKLNSLPLTSGASFFVLSKNKNEKTLAVIEACHTSAFYSSLEN
                     EIKDFMSTSTDKQIDFILNDGRNKDITRLIETLFPDKCVINYSEICGLYATNSAFAMH
                     LATDIICNKTKQVYGKTIANHKRALIINTLLPARAGFIMIKSNEA"
     gene            5701..6453
                     /locus_tag="PKU82_06485"
     CDS             5701..6453
                     /locus_tag="PKU82_06485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ82559.1"
                     /translation="MKHKIVTVIFILCIIALFVFLYQDKSFFYLLAALIFLYLTITAL
                     GSYQIKWNYFIKSVNTGKPEGISLTFDDGPDPKVTKKILDVLELAGAKATFFVIGKKA
                     EQYPDIINLIVSKGHTIGNHSWSHHAGIGMFSKQKLLADFNQCSQKIEELTGQKPVYL
                     RPPFGVTNPRYEYVLKNTGLKSIGWSIRSYDTSAKNEQKLLHRITSQLKPGAIVLLHD
                     TKDITLAILPALLNYCNQNNIAIKPLAELIEI"
     gene            6450..7073
                     /locus_tag="PKU82_06490"
     CDS             6450..7073
                     /locus_tag="PKU82_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOZ82560.1"
                     /translation="MRLLIAFILFIWIPVFAKAQQEPLNNPAELTEKLKQASISNNTI
                     KADFIEQRFMSALKEPQTSYGIFYYKKENKLRWEKTKPTNYVFISENNKVRVKENNHE
                     KDVSSYNQVVGRIKDLMLTLVNGDFSNGKQFDPQYFQTKSEYIVKLKPRNKRMSNAFD
                     YIQLSFNKKNLLLEELAFYEKSGDRNIMKFSNQMVNTELNDTLFKNF"
     gene            7075..7428
                     /locus_tag="PKU82_06495"
     CDS             7075..7428
                     /locus_tag="PKU82_06495"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82561.1"
                     /translation="MLKDFYTIINKTSGQNCVYRIKLNPAHEIFKGHFPGQPVTPGVV
                     LIQMTTELTQNAIEQNVKLKQVINSKFLNVMNPEKNPEVTFKISITKDGQLYKVATSA
                     EDEGVCFFKITAIYQ"
     gene            7434..8606
                     /locus_tag="PKU82_06500"
     CDS             7434..8606
                     /locus_tag="PKU82_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOZ82562.1"
                     /translation="MADIAACVIIPTYNNSKTLKKVIDGVLNFSSGNDVIVINDGSTD
                     NTTEILNQYNEQIVLLNNKTNSGKGYSLRKGFKEAISRGFKNAITIDSDGQHYPEDID
                     VMIENALKFPEALLMGSRNMNQESVPGKSSFGNKFSNFWFWVETGQKLPDTQTGFRLY
                     PLEPLKKIKLYTTKFETEIEVIVKLAWQDVKIIPVNIRVLYDMKERVSHFRPFRDFTR
                     ISILNTYLVTLTLFYYLPLRLFKYIRRKGIFTIIKEEIFRRDESILKKSLSVGFGVFM
                     GIVPIWGFQLLVGIPLAVLFRLNKVLFITAANISIPPMIPLIIYFSYYLGGFFVSNDM
                     KFQSMADITLQSIHANFMQYATGACVLALISGIFFMTITYITFLALKFKDKLTEKA"
     gene            8911..9432
                     /gene="azu"
                     /locus_tag="PKU82_06505"
     CDS             8911..9432
                     /gene="azu"
                     /locus_tag="PKU82_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HOZ82563.1"
                     /translation="MRKSLLALASAAFIFAACNQSKQPETPATETTPPAATEEAAPAV
                     APPTEPAIVEISGGDDMKFDKTEITVKEGQTVKLTLKHTGKSPKDAMGHNFVLLASGT
                     SLQDFATAAIKAKDNDYIPADMASSVIAHTKTIGGGETTEIEFTAPSKGTYDFLCSFP
                     GHYGTMKGHFIVE"
     gene            complement(9558..9782)
                     /locus_tag="PKU82_06510"
     CDS             complement(9558..9782)
                     /locus_tag="PKU82_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOZ82564.1"
                     /translation="MENKKHLQFKTNINCSGCVAAVKPHLDKLISSESWHVDTANKDK
                     ILSVSTNDISEEEIINTVQKAGYKIELLKK"
     gene            complement(9884..12299)
                     /locus_tag="PKU82_06515"
                     /pseudo
     CDS             complement(9884..12299)
                     /locus_tag="PKU82_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(12380..12898)
                     /locus_tag="PKU82_06520"
     CDS             complement(12380..12898)
                     /locus_tag="PKU82_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOZ82565.1"
                     /translation="MAVQKLLEDSGFITTDVQLGEVTIQVTPDEAEKKQLQDNLKKLG
                     FELIDDKKSRIIEKIKTLIIDLVHHHDGELKKNLSEVLSNELKHDYTYLSNLFSEVEG
                     TTIEKYFIAQKIERVKELLVYDELSLSEIAFRLNYSSVAYLSNQFKKVTGLTPSHFKQ
                     IKADKRKPLDKV"
     gene            complement(12949..14352)
                     /gene="mnmE"
                     /locus_tag="PKU82_06525"
     CDS             complement(12949..14352)
                     /gene="mnmE"
                     /locus_tag="PKU82_06525"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOZ82566.1"
                     /translation="MLQTDTICALITPPGRGTVGVIRISGMKTFEIISKIFTPANKTL
                     STSELEHQRIYFGKIVDKNDLIDEVLVSIFKSPATYTGEDVAEISCHGSGYIRQRILQ
                     LIMQSGAVMAKPGEFTMRAYLNGKMDLAQAEAVADIIEAENCISHHAAMQQMRGGYSE
                     EIKSLRDQLIKFAALIELELDFSEEDVEFASRAELKTLVLNVQSKIEHLLKSFELGNV
                     LKNGVPVVIAGKPNVGKSTLLNALLNEERAIVSDIAGTTRDTIEEELNVDGIRFRFID
                     TAGIRKTDDAIESIGVKRTFAKANEAKTILFLFDPMESSPADLKNEILQLQNELGISD
                     NELQKKVLFVANKSDALSTNFDMHSYDKCGVVILISAKNKSNLNLLKETLIERSGIDG
                     VALNESVVTNARHAISLSQAKSALDLFLQGMEDGISNDLLSTHIRDAIYSLGEITGQV
                     STEDLLDYIFSQFCIGK"
BASE COUNT         4863 a         2623 c         2384 g         4654 t
ORIGIN      
        1 ttttgcgatt tgttacttac tactattttt tattcgcagc aaaaccaaag aaaatcctca
       61 taaattttta tcaaagagcc ttaaaggtca atgaagttgt tgcaaaaaaa ctatgcagaa
      121 aaaactttta ctacttagga cagaccttag ttgaccgtac agcattttta cttggaaaag
      181 gcaatgtttt cagtcataat tttgaaaacg agaaatttct tgtcgaaatg caggaacaga
      241 gaaaaggtgg catactcatc agtgcccatg ttggcaactg ggaaactgcc ggaaaccttc
      301 tcaagtcaag agtttcttca accattcatg ttctgatggt tgatgcagaa gctaaaaaac
      361 taaaacaata tttagataac agcaccggtg gttcgcaatt ctccataatt gctgtgaagg
      421 acgacctttc ccatattgta aaagttaata atgcattagc cgccaatgaa ctcattgcca
      481 tgcatggtga cagatacttg ccgggcacaa aaaccattca attagatttc atgggccgaa
      541 aagcccattt tccctacgga ccgtttttgc tggcatcacg ttttaatgca cccgtcactt
      601 ttgtttttgc tcttaaagct aatgattttc actatgagtt aagtgctacc aaacccataa
      661 ctgaaaagaa atcacctgag gatattgctg ccttgtatgt tgaagaattg gaaaaaaaag
      721 tgaaaaaata tcctgaacaa tggtttaatt attacgattt ttacagcgaa tgattattga
      781 aaaagtaaat atcgaaaact atattccaca gcgctttcct tttgtcatga ttgacaatct
      841 tattcatgcc aatgaatcag gatttttaag ttcatttctg gtaaagaaag aaaatatatt
      901 tagtgaaaat ggatatttga cagaaccggc attgatagaa aatattgcgc aaacctgtgc
      961 agcaggtttt ggatttattg gtcagcaaaa aaatgaaggc ccatctattg gttacatagg
     1021 ttctatcagt aaacttacag tacaccaatt gcctgaatat ggaacaacca tattaacaac
     1081 agttgaaatt acaaacacac ttggcaatat atttttaata aagggtaaaa atacttgtaa
     1141 tgagaaatta ttggtagaat gtgaaatgaa aatagctctt gcatgaaaca acttttacaa
     1201 gtagaaattc ccattaaagt aagattcagt gataccgatg caatgggtgt ggtatggcac
     1261 ggaaactatc tgcgtttttt tgaagatgca cgcgaggcat tcggaataaa atatggtctc
     1321 acctatcttg atgtttatca tcgtggtttt ttcactccaa ttgtcaaatc atctgttgaa
     1381 cataaatcgc caattaatta tggtgaagaa gttgttgcaa tagcaaaatt tcaccctaca
     1441 ccatcagcaa aaattatgtt tgattttgac atcctgaata aaaccacagg aaaattaagt
     1501 gctaaaggtc ataccacaca ggtatttcta agtgtagttg agcgcgaaat ggaattggtg
     1561 aaaccaccat tttacattga atgggaaaat tctataggca ttaaacatta gcaagtgata
     1621 tacatagggt cggaaaccat acttagtcct aatggcaatg tggtagattg ttttaaaaag
     1681 attgccgaaa aaaaatcagg tgttagcaag ataccaggaa atggttttaa gggagagtct
     1741 ttctattgtt ctatgtactt agagaacaga ccggacttta acacattagc tgcacaaact
     1801 attgaacaat tattgcaaaa atcgaaagcc aatctgctta ctgaagaatc tactcttctt
     1861 atcatcagta ccaccaaagg cgatttatca aaaggatttg aaaatgcaat aatcagtccg
     1921 gcaaaattcc tttacgaaaa attaaaacta aaacattttc cactcatcat ttccaatgca
     1981 tgtgcgtctg gcgtcatggc aatcaatgca gcagcagcgt ttattgaaaa caaacaatat
     2041 caaaatataa ttgtacttgg tattgacatg ataacagact ttatcgttta tggctttcaa
     2101 tctctttatg ctattagtga tgagcattgc aaaccgtttg acaatgatag aaaaggaatt
     2161 aatttaggtg aaggtgccgg tgcagtttta ttatctgcaa gtaaagatgt ttttacttca
     2221 acacctgttt gctttttagg tggaagttca agtaatgatg ccaatcatat ttcgggacca
     2281 tcaagaacag gcgaaggatt atttcgcagt ataaataaaa catttaattc ggctggcatt
     2341 aagacggaag atatagattt tatctctgca catggcacag ctacagtgtt taatgatgaa
     2401 atggaagcaa tagcttttaa caggttaaat atgcaacaca ttccggtaaa cagtttcaaa
     2461 ggatttacag gacatacact tggggctgcc ggtgttattg aaacagcttt atgcataacc
     2521 tgtatgcatg aaaacaaatt acttccttct gccggttatt ataacaaagg gacaaccgtt
     2581 gagcttaatt tgataaaaga tgctgcttct gctacaataa acactgtctt aaaaacagca
     2641 tccggctttg gcggatgtaa tgcatcacta ttattgcaaa agataaaatg aaaataacag
     2701 gttgctgtag aattcatcaa aacgaagttt tagttaatgg taaaaaatat tttacctctg
     2761 acaagaatca gcatgaagag ctatcaatgc agctttaccg attcctgaaa acagagtatc
     2821 ctaagtttta caaaatggat ttgctctcaa aaatgggatg gcttgcaacg gaaatagttt
     2881 gtcagacaat tcctacgata tctaaatttg ctgacgatga aatcgcattg ttatttgcca
     2941 acagccactc ttgttcagaa tcagatgagc gatttaatca ttcctttaaa gtagagaata
     3001 gtccttcacc ttctcagttt gtatataccc ttccgaacat acttttaggt gagttggcaa
     3061 taagacataa atggtatggc gaaaatttgt ttaccatact acctgaattt tctgccgagc
     3121 atttcagttc tcatgcaggt atttttattc ctcaaaaagc aaaagcatta ttctgtggat
     3181 ggataaacct ttttcctgta acagatgtat ttgttttctt tgcagaagct aattcagaaa
     3241 catcaccggg aattttggat agtaaagaaa ttgaacattg gtataaaata ataaagtgaa
     3301 atggataatt taaaagaaga attaaagaag catttaatta cacaactgaa tttggaagat
     3361 aaaacaccgg cagacatcag tgatgaagct cctttgtttg gtgatagcgg cttgggactc
     3421 gattcaattg atgcattgga attgatagta cttttagaca aacactatca cattaaactc
     3481 acaaacccag aagaaggaaa atctgtattc tattctatca gtaccatggc agaatacatt
     3541 gagaaaaata aaaagtaaat ttcgatgaag gtttatgtca ccggcattgg tgtagtttct
     3601 gcaattggtt taacagttga agaaaattat cattctttat taaataagaa gagcggtata
     3661 actgcattta aattcccgga agaacaaaaa tctattcaag caggaagcat tgcaaaatca
     3721 aatgctgaac ttaaacagat gttatctata actaatggta atttttcaag gacttcatta
     3781 cttggaataa ttgcagccga acaagctatg ggtaaaaact ttcacaataa aaaattacgt
     3841 accggtttta tttcatctac atctgttggc ggtatggata aaacagagaa gtattacagg
     3901 caggtcttaa agaatctacc tgttgatttt aatctgacac gaactcacga tagtggaaat
     3961 acttcagaaa atattgcagc ttacttagga ttttcaggtt acattaatac actttctaca
     4021 gcatgttcat cagccgctaa tgccataatg cttgcagcac gaatgataga gcaaggaaaa
     4081 atggacagag ttttagttgg tggaactgat ccgataacag attttacaat taaaggcttt
     4141 caatcgttga tgatatatga tgatgaatgg tgcaagccat ttgatcataa cagaaacgga
     4201 cttaacctag gtgaaggcgc aggtttttta ttgttggaaa gtgatcacag cataaacatt
     4261 tcgggcaatg aaccaatttg cagtttaaca ggatggcata atgctgccga tgcctatcat
     4321 cagactgctt catcacccga tggtaaagga gccacaattg caatgaaaaa tgcattgaag
     4381 aaagcagggc ttgggcctaa agaaatttct tacatcaacg ctcatggcac agggactaaa
     4441 aacaatgatc ttagcgaatc cattgcaatt aaaaatgttt ttggcgagtc tataccacct
     4501 tttagttcaa ccaaggcata cacaggtcat acattggcag cagccggtgc aattgaagca
     4561 gtatattctg ttcttgcaat aaaaaatcaa atccactttg caggattaaa ctacaatgac
     4621 cctttggagg aaacactcat gcatcctgtt acagaaccaa caaaagctgt aacaaaacat
     4681 gtgatgtcaa actcttttgg ttttggcgga aataattctt cactaatttt ttcaggcatt
     4741 tgattatgta ttgtattcaa tctgcttcca ccatttctca tcaaccttcg tttgcaagca
     4801 aagggttttc ctcgaatctt tctaaagaaa ttgaatcatt aattaaacct gattacaaag
     4861 agtatgttcc gcctgcatta atcagacgga tgagtgaaat actgagaatg tctgttgcct
     4921 gcagtaatct tgcattaaaa gaagctggca ttgaacaacc tgatgcaata ataaccggta
     4981 caggattggg ttgtctttat gacactgaaa aatttctaaa caatgtaatt accattgaag
     5041 gcgggctgct ccctcctact tcattcattc aatctactca caacactatt gcaggtcaga
     5101 tttcattggt tcttggcaac catcaataca atatgacaca cactcaaaac agtctgtcgt
     5161 ttgaacatgc gttgctcgat actatgctaa tgcttgatga aaaaaatgga aatattctta
     5221 ttggcagtgc agacgaggat attgaagcat tacatcctat tgcagcacat tttaaattaa
     5281 actcgttacc acttacaagt ggtgcttcat tttttgtctt atcaaaaaat aaaaacgaaa
     5341 aaactttagc tgttattgaa gcatgtcata cttctgcttt ttattcatca cttgaaaatg
     5401 aaattaaaga cttcatgagc acatcaacag ataaacaaat agactttatt ttgaatgatg
     5461 gacgtaataa agatataaca agattaattg aaacattatt tccagataag tgcgtcatta
     5521 attattctga aatttgcgga ttgtatgcta caaattctgc ctttgcaatg catctggcaa
     5581 ctgatataat ctgcaataag acaaaacaag tttatggcaa aacaattgct aaccataaac
     5641 gggctttaat aataaacacc ttgctacctg cacgggccgg attcataatg ataaaaagca
     5701 atgaagcata aaatcgtaac tgtaattttt attctctgca ttattgcatt atttgttttc
     5761 ctttatcagg acaaatcctt tttttattta ttggcggcac ttattttcct ttatctgaca
     5821 attacagcat tgggctccta tcagattaaa tggaactatt ttataaagtc agttaataca
     5881 ggaaaacctg aaggtatatc acttacattt gacgatggac ctgatccaaa agtcactaaa
     5941 aaaatattgg atgttcttga actggcaggt gcgaaagcaa cattttttgt gattggcaaa
     6001 aaggctgaac aatatccgga cattattaat ctaattgtca gcaagggcca cactataggc
     6061 aaccattcat ggtcacatca tgcagggatt ggcatgtttt caaagcaaaa gttgttggct
     6121 gattttaatc aatgcagtca aaaaatagaa gagcttacag gacaaaaacc agtttatcta
     6181 agaccaccat ttggagtaac taatcccaga tatgagtatg tgttgaaaaa tacaggattg
     6241 aaatctattg gatggagtat cagaagttat gacacctcag caaaaaatga gcagaaactt
     6301 ttacaccgga taacttcaca attgaaaccc ggagcaattg tacttttgca tgacacaaaa
     6361 gatataacac ttgccatttt accggcattg ctaaactatt gtaatcaaaa taacattgcc
     6421 attaaaccgt tagctgaatt aattgaaata tgagattgtt aattgcattt atactattca
     6481 tttggatacc tgtttttgcc aaagcacagc aggaaccatt gaacaatccg gctgagttaa
     6541 cagaaaaact caagcaagca agcatttcaa acaatacaat taaagcagat tttattgaac
     6601 aacgatttat gtctgcattg aaagagccac aaacatctta tggtattttt tattacaaga
     6661 aagaaaacaa attgcgatgg gaaaagacaa aacccacaaa ttatgttttt atcagtgaga
     6721 ataataaagt acgtgtgaag gaaaataatc atgagaaaga tgtcagttcc tacaatcagg
     6781 ttgttggaag aattaaagat ttaatgctga ctttggttaa tggtgatttt agtaatggca
     6841 aacaattcga tccgcagtat tttcaaacca aatcagaata tattgttaaa ttaaagccaa
     6901 gaaacaaaag aatgagcaat gcatttgatt acatacaact ttcattcaac aaaaagaatt
     6961 tattacttga agaattggct ttctatgaaa aatcaggtga caggaatatc atgaagtttt
     7021 caaatcaaat ggtaaacaca gaactgaacg acacattatt taaaaacttc tgatgtgctg
     7081 aaagatttct acaccatcat caataaaaca tccgggcaaa attgtgtgta tcggattaaa
     7141 ctaaatcctg cacatgaaat atttaaaggt cattttccgg gacaaccggt tacgccaggt
     7201 gtggtattaa ttcaaatgac aacagaactc acacaaaatg caattgagca aaatgtaaag
     7261 ctgaagcaag tcattaacag caaattttta aatgtaatga atcctgaaaa gaatcctgaa
     7321 gttacattta aaatcagtat tactaaagat ggtcaattat acaaagtagc aacatctgct
     7381 gaagacgagg gtgtttgttt ttttaaaatc accgccattt atcagtgaat aaattggctg
     7441 acatagctgc atgtgttatt ataccaacat ataataattc caaaacgcta aagaaagtta
     7501 ttgatggtgt tttaaatttc agctcaggaa acgatgttat tgttatcaat gatggttcga
     7561 cagataacac aacagaaatt cttaatcaat ataatgagca aattgtttta ctaaacaaca
     7621 aaacaaattc cggcaaaggt tattcgttgc gtaaaggttt taaagaagca atttcaagag
     7681 gatttaaaaa tgcaattacc attgattctg acggacaaca ttatcctgaa gacattgatg
     7741 taatgattga gaatgcccta aaatttccgg aagcattatt gatgggttca agaaatatga
     7801 atcaggaatc agtgcccgga aaaagttcat ttggtaataa attttccaac ttttggtttt
     7861 gggttgagac gggacaaaaa ttacccgata cacagacagg atttcgtttg taccctcttg
     7921 aaccattaaa gaaaattaaa ctttatacaa ctaaatttga aacagaaatt gaagtcatag
     7981 taaaacttgc atggcaagat gtaaaaataa ttccagtaaa tatcagagtg ctttacgaca
     8041 tgaaagaacg ggtatcgcat tttcgccctt ttcgtgattt tacaagaatc agtatcctga
     8101 atacttattt agtaaccctg acactttttt attatttacc attacgatta ttcaaataca
     8161 tcagaaggaa gggaattttc acaattataa aagaagagat tttcagacgt gatgaaagca
     8221 ttttaaaaaa atcactttcc gttggttttg gagtatttat gggcattgtt ccaatctggg
     8281 gatttcaatt gcttgtaggt attcccttag cagttttgtt tcgattaaat aaggtgctat
     8341 ttataactgc tgcaaacatc agtattccac ccatgattcc tttaattatt tattttagtt
     8401 attatctcgg aggtttcttc gtttcaaacg atatgaaatt tcaaagtatg gctgatataa
     8461 cattgcaatc tattcatgca aattttatgc aatatgcaac cggtgcctgt gtgcttgctt
     8521 taatctccgg tatttttttt atgacaatta cgtacattac atttttggca ctgaagttta
     8581 aagataagct tacggagaaa gcataattct cagatttatg taaaaatttc aaactgacta
     8641 aaatcattca ttcgtaaaaa ttaattaaaa ttaaaccctt atctatttaa ttacgagtgt
     8701 tttaaatgaa tgttcggcat atctaaattc taataaagtc gcatctgtaa actgttcata
     8761 attaacggta gtttaatact tgtattttgg aaagaactaa ttgatgctat acatttgcac
     8821 ctgtttttag aacaatagtt caatcgctat taattcagat attattacaa acaattatac
     8881 aatatataac aatcaaataa atacattaaa atgagaaaat cacttttagc attagcatca
     8941 gctgctttta tttttgcagc atgtaatcaa tcaaaacaac ctgaaactcc tgcaacagag
     9001 acaacacctc ctgctgcaac agaagaagca gcaccggcag tagctccacc aactgagcct
     9061 gcaatagttg aaatatccgg tggagatgat atgaaatttg acaaaacaga aatcactgtt
     9121 aaagaaggac aaactgttaa gttaacttta aagcacacag gaaaatcgcc taaagatgca
     9181 atgggacaca attttgtatt gctagcatca ggtacttcat tacaagattt cgcaacagct
     9241 gcaattaaag caaaagacaa cgattatatt cctgctgaca tggctagtag tgtaattgca
     9301 catactaaaa caattggtgg tggcgagaca acagaaattg aatttactgc tccatcaaaa
     9361 ggaacttatg atttcctttg ctcttttcct ggtcactatg gtaccatgaa aggtcacttt
     9421 atcgttgaat aatttaactg ataacattta cccgccaaac atctgatttg actgtttggc
     9481 gggtattttt ttaagtaact ctcattatat tgtttcaata actcaagact tttaacacaa
     9541 attccttcag aagtctttta cttttttagt aattcaattt tataaccggc cttctgaaca
     9601 gtattaataa tttcttcttc tgaaatatca tttgttgaaa cagacaatat tttatccttg
     9661 ttagctgtat ctacatgcca actttcgctg ctgattagtt tatctaaatg tggttttact
     9721 gcggcaacac aaccactaca gttaatgttg gtcttgaatt gaagatgttt tttgttttcc
     9781 attttattta agtattaata aattgctttt ctgtgtgcaa agtttgttat tttgtttgag
     9841 tgacaggtta caacattata atttttattt gtaaaatata ctgctacttt ttccatttta
     9901 atctcaaact attactcaca acgctaacac tactcatagc catagctgca ccggcaatca
     9961 ttgggtttaa aagaaatcca ttgattgggt acaaaatacc tgctgcaatt ggaattccaa
    10021 tgacattata aataaatgcc caaaacaaat tttgcctgat tgttgcaaca gtttgttttg
    10081 acagccgaat ggccattgga attttattta aatctgagga aacaattgtc atttttgcaa
    10141 catccattgc aatatcacta ccctttccca ttgcaatact aacatcagca gtagctaatg
    10201 ctgtactatc attaattcca tcaccaacca ttgctactac ttttccttgt tgctgcaaca
    10261 ccttaataaa atcggccttt tgatgtggca acatacttgc ctgatattta tcaatacctg
    10321 tcttttctgc aattgctttt gcagtagatt cgttatcacc ggtaagcata taaagctcaa
    10381 tacccatttt atgtagttgc tgaatagcag ccactgacgt tggctttata ctgtctgaaa
    10441 ttgcaataat ggacaacact tgcttactat cggcaaacca aataactgtt tttgattcct
    10501 ttgtccatgc agtagctttt tcgactttca tatcttctat taaaatattt tgctccttta
    10561 tgaaagtcat atttccaaca aaataattct ctttctgata tgttgctctt acacccttgc
    10621 cggtaatgct ttcaaatgaa gttagttcta tatcttctgt cttagtgaaa ttctttacta
    10681 ctgcatctgc taatggatgt tcagacttct tttcaatact atagaggatt gatgcagtac
    10741 ttgaattatc attataccaa taaacatctg taacaaccgg tttaccctca gtaattgtac
    10801 cggttttatc taaaacaaca gttgtaattt tctttgcaag ttcaaggctc tctgcatctt
    10861 ttataagaat tccattttca gctgctttgc caacaccaac cataattgca gttggtgttg
    10921 caagacctaa agcacaagga caagcaatta ccaataccgt tactgctgcc aacaatccct
    10981 gcactaatcc attatcaccg ccaaaaattt gccaaagaac aaatgttagt attgaaatgc
    11041 ctataactac aggaacaaat atccctgcaa ttttatctac taatttttgt accggagcct
    11101 tactgccctg cgcatcctga acagtcttta tgatttgcgc cagcatggtt tcctttccaa
    11161 cctttagtgc attaaactga aagctgcctt tctgattgat tgtacctgca aacacgttgt
    11221 catttatgtt tttttaaaac gggaacaggc tctcctgtca gcgtactttc atctacgtat
    11281 gaactgccat tgataagttt gccatcaact gcaatttttt cgccaggttt cacaagtatg
    11341 atatcatcaa caatcacatc ttcaacgaaa gtttgctttt ctgttccatc cgactgaatt
    11401 acaataactg ttttaggctg caaccccatt agttttttaa ttgctgaaga tgtattcccc
    11461 tttgccttct cttctaacat tttgccaaga agtataaatg caactattac agcagcggct
    11521 tcaaaataaa cgtgagcgtg aagtcccctt tgatgccaga attgaggaaa tagcatattg
    11581 aacacactaa acagatatgc aattcctgta ctcagtgcta ccaatgtatc catatttgca
    11641 gagcggtgtt ttgcttgttt ccatgcattg ataaaaaaat ccttaccaag ccatagaact
    11701 acaggggttg aaaacagcca cataatttca ttagcataag gtatatccat aagaaacata
    11761 ccaattatta caacaggaat tgatagtgca atagctaaaa cagtcttctg ttttaacttt
    11821 ttaaacttct cctgctgtat gttttcaaaa ctattctgtt gtgatgcctc atcttctatt
    11881 agcaaatcat atccaacctc ttgtaatgcc ttttgtaatg ccagcggatt ggtcatgttt
    11941 gggagatatt caactgtaag ttctgttgtg gcaaaattta cagaagcatt aattacacca
    12001 tttaccgatt gaatcatact ctcagcacta actgcacatg aagcgcatga catatttaat
    12061 actggaaact tttttcttac ggttgttacg ccatatccaa ggtctttaat tgttgcaatg
    12121 acatctttaa tagtttcttt atttgaaaca gtaattacag cacgtctgtt attcaattca
    12181 actttatgtg aatcaacacc tgcaaccttg gaaagccctt tatcaactat cagtgcgcaa
    12241 tgttcgcttt ctacattttc taaaggaaga aaaattgtgt catcagtatt cttttccata
    12301 tcaaagcaaa tttacactgc aaaggtgcaa agaatgaaat caaaaaacgt tatagtttta
    12361 tggaaatgaa ttgtaaaatt tacaccttgt ctaaaggttt gcgtttgtcg gctttaattt
    12421 gcttaaaatg acttggagtt aatcctgtta ccttcttgaa ttgattactc aaataagcta
    12481 cactagaata attcagtctg aatgcaattt cacttaatga caattcatca tacactaata
    12541 gctctttaac acgttctatt ttctgagcaa taaaatactt ttcgattgta gtaccttcta
    12601 cttctgaaaa aagatttgac aagtatgtat aatcatgctt taactcatta ctcaatactt
    12661 ctgaaagatt cttttttaac tcaccatcat gatgatgaac caaatcaata attaatgttt
    12721 ttattttttc tatgattcgg cttttcttat catcaatcaa ttcaaaaccc aactttttta
    12781 aattatcctg caattgtttc ttttctgctt catctggtgt tacctgaata gtcacttcac
    12841 ccaactgaac atctgttgta atgaagccac tatcttccag caacttttga actgccataa
    12901 tacagcggtt gcaaaccata tttttaatgt aaagtttatg agacatattt actttccgat
    12961 acaaaattga ctaaagatat aatcaagtag gtcttcagtt gacacttgcc ctgtaatctc
    13021 ccctaaagaa taaattgcat ctctgatgtg tgtcgaaagt aaatcattgc tgattccatc
    13081 ttccattccc tgaagaaata aatccagagc agattttgcc tgcgataacg atattgcatg
    13141 tcttgcattg gtaacaacag actcattcaa agcaacacca tcaatacctg agcgttctat
    13201 taatgtttct ttcaataaat tgagatttga tttattcttt gctgatatca atattaccac
    13261 accacattta tcataactat gcatatcaaa gttagtactt aatgcatcag atttatttgc
    13321 aacaaacaaa actttcttct gcaattcatt atcagaaatt ccaagttcat tctgcaattg
    13381 caaaatttca ttttttaaat ctgcaggtga tgattccatt ggatcaaaaa gaaataaaat
    13441 agtctttgcc tcatttgctt tagcaaatgt tcttttcaca ccaatgcttt ctatggcatc
    13501 gtcagtctta cgaatacctg cagtatcaat aaatctaaat cgaattccgt caacattcaa
    13561 ttcttcttca atggtgtcac gtgttgtgcc agcaatatca ctcacaattg cccgttcttc
    13621 attgagcaat gcatttaaca aggttgactt accaacatta ggcttgccgg caattacaac
    13681 aggaactcca tttttcaaga cattgcctaa ttcaaaactt ttaagcagat gttcaatctt
    13741 actttgcaca tttaaaacaa gtgtttttag ttctgcgcga ctggcaaact caacatcttc
    13801 ttcactaaag tcaagctcaa gttcaatgag ggcggcaaac tttattaact gatcgcgcag
    13861 tgattttatt tcttcagaat aaccgccacg catttgctgc atggctgcat gatgcgaaat
    13921 acagttttct gcctcaatga tatctgctac agcttccgcc tgtgcaagat ccatcttccc
    13981 attcaagtaa gctctcatgg taaactcacc gggttttgcc atcacagcac cagattgcat
    14041 aatcagttgt aaaattcttt gtctgatata acctgaccca tgacaactaa tttcagcaac
    14101 atcttcaccg gtatatgttg caggagattt aaatatggag acaagtactt catcaatcaa
    14161 atcatttttg tcaactattt ttccaaaata aatacgttgg tgttctaact ctgaagttga
    14221 caaagtttta ttagctggag taaatatttt tgaaataatt tcaaatgttt tcattccaga
    14281 tattctgatc acaccaacag taccacgacc tggtggtgta attaaagcac aaatggtgtc
    14341 agtctgtaat atcatttttg ttctataata aaattattaa gccgattagt aagttgcatg
    14401 ccaaaattag ttccaaatac tttaccctgc attttattca ccggtctgat acctgagcct
    14461 gtattggtaa gaaatatttc atcaacatca attaattgtt ctattgaaat atcctgttct
    14521 atag
//
LOCUS       DAOOOL010000062        14368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_362_length_14368_cov_77.605743, whole genome shotgun sequence.
ACCESSION   DAOOOL010000062 DAOOOL010000000
VERSION     DAOOOL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_14368_cov_77.605743"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..86)
                     /locus_tag="PKU82_06530"
     tRNA            complement(2..86)
                     /locus_tag="PKU82_06530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ser,seq:tga)
     gene            192..1643
                     /locus_tag="PKU82_06535"
     CDS             192..1643
                     /locus_tag="PKU82_06535"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOZ82567.1"
                     /translation="MKRLLLFLALGSIASLTQAKTIKALFDYKSFYAPGQGSYLEWYL
                     SVKGTSVNYKMSESGNYEASVAVAYKISQNDKVVAQDSYNLISPLTKDTLLVSDFIDN
                     KRLQLPNGKYNIELSVKDNVADAKTFTINQGFELAFSPGKTAVSDIELIQNYSKKETG
                     NSALIKNGYEIIPRVDNFYQTHEERLIYYAETYFTNSLTDDAVAVYSYLQNAESGNTI
                     MDYGTVQRKKTSEVIPLLSEINIANLGSGNYNLVVEIKDKNNQSLALSKIFFQRQSVV
                     KDNFALNNDVSSTFVATMNDKNVLAENIACLQPISNAQQSEWIGNQLKAANIDMMKNF
                     FYDFWQRREPADPQKAWNVYKTHVDAVQQKFGSRALKGYNTDMGRVYLAYGAPSNINK
                     GEYEPNTYPYEIWRYDKINNHTNKTFVFYSKDLLARNFTLLHSDMPGEPYEAAWNMIL
                     HDRSIKAKNIDDTGQDLKGVYSGDRTNDNFNAK"
     gene            1650..2501
                     /locus_tag="PKU82_06540"
     CDS             1650..2501
                     /locus_tag="PKU82_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOZ82568.1"
                     /translation="MTFLFLKFVIRCIAFLDLRILYLLADGIAFCFKYIFPYRKKVIL
                     QNLYNSFPEKSEQEIEEIAKQFYNYFANLIVETIKMSSISKEELLRRWRFTNAEVLHS
                     YYEKNRSIIAVCGHFNNWELGALALSVIDKHQMLGVYKPLTNKSWDEYFRTIRSRFGM
                     IPVPMKSILRELIKRKNEPTITTLAADQTPHRSEINYRTTFLNQDTAVFLGAEKLCKQ
                     TGHVLIYFSMHQIRRGYYDITIIPISDDPKNTAEYELTHKHLRLLENDIHAQPGNWLW
                     SHRRWKY"
     gene            2515..2799
                     /locus_tag="PKU82_06545"
     CDS             2515..2799
                     /locus_tag="PKU82_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOZ82569.1"
                     /translation="MNVLKQFRLIAIAEGISFLVLLFIAMPLKYGADMPLAVKYTGWV
                     HGLLFVFYVYSLLQVWQQYKWNLKMMSLAMLASVLPFGTFYFEKKYLNNI"
     gene            2831..3853
                     /locus_tag="PKU82_06550"
     CDS             2831..3853
                     /locus_tag="PKU82_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ82570.1"
                     /translation="MKAAVVILNYNGAALLQQFLPSVIANTPSWCSVIVADNASTDNS
                     LSVMKKHFPDVRCIEMQQNTGFAGGYNNALQHVDAEYFILLNSDIEVTPQWIEPIIEF
                     MDKHADVAACQPKILAYHRKEYFEHAGACGGYIDYLGYPFCRGRIFNSLEKDENQYSD
                     NKEIFWATGAAMFIRSHVFKSAGGFDTDFFAHMEEIDLCWRMKHSGHKLYCIPASRVY
                     HVGGGTLHKSNPKKTYLNFRNNLMMLLKNLPSGNRFSVFTKRMYLDKLAFIKFFISGN
                     FKDAWAVYRAYFYVILHFKTLKKKRVEIKNINTNVSCIFEKSIVAEHFFRGKKKFTEL
                     NQDCFS"
     gene            3874..4485
                     /locus_tag="PKU82_06555"
     CDS             3874..4485
                     /locus_tag="PKU82_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ82571.1"
                     /translation="MKIICVGRNYAAHAAELKNPLHSEPVIFMKPETAQLLPRMPFFI
                     PDFSTDIHHEVELVVKINRTGKHIQEQFANTYYDEIGIGIDFTARDKQSQLKEKGLPW
                     ELAKAFDGSAPTGKFLNKSLFKSVEDINFRLEKNGNIVQQGNSSNMLFNIDKIIAFVS
                     SYITLKKGDLIFTGTPEGVGKVQANDVLDAFIEDQHLLTVKIK"
     gene            4585..5964
                     /gene="mgtE"
                     /locus_tag="PKU82_06560"
     CDS             4585..5964
                     /gene="mgtE"
                     /locus_tag="PKU82_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOZ82572.1"
                     /translation="MLQQQQKEEIKSLLREADLAGCKAYLENWLPAELVELIDESPNE
                     HRVLIFKAINREQAFETYESLDLNLQISLLDILPPRQVQLILNDMSPDSRTALLEDID
                     NDLLNRLLKLLTPKERAIALSLLGYPENSIGRLMTPDYIAIRKEWSVQYALDYIRENG
                     ENSETLNIIYIVDDKGHLIDDIKVAEILLADLHIKIEELIDGKYTALSVTDDEKVAIS
                     EFKKHNRVALPVVDTSQMLLGIITVDDVLQLAEQEYSEDIQKLGAVEALEEPYMEVPM
                     YRLLQKRAPWLIVLFIGEMFTASAMSFFEHEIQRAVVLALFIPLIVSSGGNAGSQAAT
                     LIIRALALGEVGMKDWWRVMRREIISGLMLGLILGSIGFLRVAAWAMFTDIYGPHWLL
                     IGITIGITLIGVCLWGTVMGSMLPIVLKRFGADPATSSAPFIATLVDVTGLVIYFSIA
                     ATILSGTLL"
     gene            complement(5968..6864)
                     /locus_tag="PKU82_06565"
     CDS             complement(5968..6864)
                     /locus_tag="PKU82_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82573.1"
                     /translation="MKNVLFILLAFVITTSCKKKDDDNEAQLIFKFRFDSTQQRQDNF
                     GNAATLPANHGALSPVFNSMSSHYIELAPTAFTQLGSGAVLYRNSETTQGGDNAIVFS
                     ESVLAGNNEEFFRVPFKNIAPGTYQYLRVSLAYQNYDITFGANILGTVYPNLKGTVAS
                     FIGFNSFINSFKIKDSTVTLNANRKQGYWAFETLNQNYPFPGFLPPVISGQAPAGATT
                     VPNPLASTSPIPAGSCVVTGVFSAPFVVTGNETSDKTITVSISTNKSFEWVEHSTPGV
                     YEPANGDTVVDMGVRGLIPIIN"
     gene            complement(7290..7727)
                     /locus_tag="PKU82_06570"
     CDS             complement(7290..7727)
                     /locus_tag="PKU82_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82574.1"
                     /translation="MKKSKYYKTAKITDMKELKMCPITKCEPGAKLIGVKIDNDVFDF
                     LKENIMVDEDLFDVMTAVSNPEKRFRFSNVCLETGCKHWTGAACGCVGKVLKNVEDLT
                     LKKDAILPQCSIRERCRWFYQEGRMACDVCPLLAIKDKDLLEV"
     gene            complement(7912..8661)
                     /locus_tag="PKU82_06575"
     CDS             complement(7912..8661)
                     /locus_tag="PKU82_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ82575.1"
                     /translation="MINLVLVDDEVNQINLIKDCVENYFPQIKILGTASSVPEAETLI
                     RSTKPDLVFSDIDMPPYTGFDLLQRFEKIDFGVVFITGFDKYAVRAFEVAAVDYLVKP
                     VTPEDLQRAIKKFEVEAERKRSAEMLQVLLHNHNISQKDEMHIALPKFSGFDVLKLKQ
                     VIHIRGEGNYSRFFLTDNTERLVTKQLGVYEKALTDFDFFRVHKSNMVNLAHVKSVSH
                     NNGGEVIMSNGNTIEIARGRKEDFLLRLKPI"
     gene            8885..11713
                     /locus_tag="PKU82_06580"
     CDS             8885..11713
                     /locus_tag="PKU82_06580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOZ82576.1"
                     /translation="MLSLLWIAPWITSATNNTTGHFTLPAGISSEDYQQGKVIFKVKD
                     SYRSLCTATGINIQPLNVALNNIGCTAVSKMFPGKEKPTTERNAMHQQYADLSLIYVA
                     QINPSASIEKAINAMLATGVLIYAEPSYIYKTNFTPNDGQIGSQWFLTKIQAFSAWDT
                     QQGDTSVVIGIVDSGTDWDHPDLQNNIKYNYADPINGVDDDNDGYIDNYRGWDMSMND
                     NNPMVDMSDHGSHVSGCAAATTNNGTGVASPGFNCKFLPVKCANATSVTTIDRGYEGI
                     VYAADHGCQIINCSWGGSAGGSVGQDAITYATVNKGALVVCSAGNTYTDIAQYPAAYN
                     YAFSVAATNSSDEKSSFSTYNYSVDISAPGSNIFSTLYNNSYASLDGTSMSAPITSGC
                     AAIVKSQFPTYNNMQVAEQLRMTADNIYGVSGNSGFVDKLGTGRVNLYNAVTQSPKGV
                     RMDNISLSDNNDLAFVAGDTLRITGDVLNLLAPLSNLTVTLSTVSSSVTILNNTINPG
                     AMATLDVVNTATTPFLVKIKPTAPVNSNVVFKLSFTDGNLIVNQYFTVVVNVDYINVN
                     INEVATTITSKGRLFYYGINQTSGLGFDFNGDNLVYDGGLMIGNNNLVSDNCRDGSNF
                     DDDFQKQIAIKAIIPSVKSEFDLTTNFNDNNVTAANRLKVLVRHNTYAWSSPGNSRFI
                     IVEYHIKNTGTNALSTLYAGICADWDIQTYANNKSDVDAVNKMGYSWCTDNGGLYAGV
                     KLLSATPFNSYAIDNVTGGAGGVDATSDFTNSEKYTTLSTSRAQAGNTLPAGNDVIQV
                     VSSGPFNLAGGDSITVAFALIAGDSLADIQQSAANAQIKYDGILTSVQENIAHGSETQ
                     LLNPYPNPNSGNELNIPFYIAKNQINSVNISLTDMTGRTVVDLQNIQTISGMNIQTVN
                     MAGLSNGIYLIKLESAGKIYTGKISVQH"
     gene            11867..13960
                     /locus_tag="PKU82_06585"
     CDS             11867..13960
                     /locus_tag="PKU82_06585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOZ82577.1"
                     /translation="MSIASASFAENKDLKEQSANPLLQPWTGPYNGVPAFDKVKEEYF
                     IPAFEAAFAEKKAEIQKIASNKEAPNFKNTIEAMEKAGATLNRVRAIYDIWSSNLNTP
                     VMTAIQEKIEPQIAAFNDEVIQNKPLFLRIEKVCSDTVGKKYSVEQHQLLKRYYNNFV
                     LAGAKLNDADKAKVANINSRLATLFTQFSKNQLADENEKYIEIKNESDLAGLPDDIKS
                     AYAKNAKERGKSTWTVANTRSAVEPFLTFSNNRELRKQVWTMFINRGDNGDANDNNKI
                     IPEILKLRYERAKLMGFATFAHQKLSDKMAKTPEATMKLMESVWPAAIARVKEEVADM
                     QAIADKENAGIKIEPWDYRYYAEQVRKSKYDLDQNEVKQYLQLEKLREGMFFVAGELF
                     NLSFTQVHDVPVFHPDVTVYKVEDKTTHKLKGIWYFDPYARTGKRSGAWMTAYREQEK
                     LSGNVTTIVSNNCNYIKGSGNDPVLISWDDATTLFHEFGHALHGLCSDVTYPTISGTN
                     VARDYVEFPSQVLERWLPTSEVLQKYAINAKTGQPLPQSLLEKLNKSSKFNQGFETTE
                     YLSSALVDMKIHLAGDKVTDPDKFEKETLAALNMPSEIVMRHRLPQFGHIFSSDEYAA
                     GYYSYLWSDVISADATEAFTEAGSLYDKKTAEKLYRNVFSVGASIDESIGYRNFRGHD
                     PDVKALMRSRGFPEK"
     gene            14091..>14368
                     /locus_tag="PKU82_06590"
     CDS             14091..>14368
                     /locus_tag="PKU82_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82578.1"
                     /translation="MVFSAIVLEWNMHKRDLDFIRITVFAKKTLHEEENRANKYVEWL
                     GNMLSRQTSYSAIDDSLLLTSQKPVSAFYSFVYLNDSLIYWDNNAVSFS"
BASE COUNT         4624 a         2635 c         2725 g         4384 t
ORIGIN      
        1 gcggagagag agggattcga acccccgttg gttttaccca aggcggtttt caagaccgct
       61 gcaatcaacc actctgccat ctctccgggc gcgaaagtaa tacaattttt aattcgcaaa
      121 ttttttttga cgattttaca acactaattc ttaaaatctt gtctatcttt atactctaaa
      181 taattcatac aatgaaacgc ttattattat tcttagccct tgggtctata gcatcgctga
      241 ctcaggctaa aacaattaag gccttgtttg attataaatc cttctatgct cccggtcagg
      301 gttcgtatct ggaatggtat ctgtctgtaa aaggtacttc cgtaaactat aaaatgagcg
      361 aaagcggtaa ctacgaagcc tcagtggctg tagcttataa aattagccaa aacgacaaag
      421 tcgttgcaca ggatagctat aaccttatca gtccgcttac aaaagacaca ctcttagtat
      481 ccgattttat tgacaataaa agattgcagt tgcctaatgg aaagtataac atcgagttat
      541 cggtaaaaga taatgtggca gatgctaaaa cctttaccat aaatcaggga tttgaattgg
      601 ctttttcacc gggaaaaact gcagtgtctg atattgaatt gatacagaat tattctaaaa
      661 aagaaaccgg caatagtgct ttaattaaaa atggatatga aataattcct cgtgttgaca
      721 atttttatca gacacatgaa gaacgtttaa tctattatgc agaaacctat tttacaaact
      781 cccttactga tgatgccgtt gccgtttaca gttatctgca gaatgcagaa tcaggcaaca
      841 ctataatgga ttatggaact gttcagcgta aaaaaacaag tgaagtaatt ccgctgttga
      901 gcgaaattaa tatagctaat ctgggttcag gcaactacaa tcttgtcgtt gaaataaaag
      961 ataaaaacaa tcagtcattg gcattatcaa aaatattttt tcagcgtcaa tctgtagtga
     1021 aagataattt tgcattaaat aatgatgttt catcaacctt tgttgccaca atgaatgata
     1081 aaaatgtgct tgcagaaaat attgcctgtc tgcaacctat cagcaatgcg caacaatcgg
     1141 agtggatagg caatcagctt aaagctgcca acattgatat gatgaaaaat ttcttttatg
     1201 acttttggca acgcagagag cctgccgatc cacaaaaagc atggaacgtt tataaaacgc
     1261 atgtagatgc cgttcaacaa aaattcggtt cacgtgcctt aaaaggttac aatactgata
     1321 tgggaagagt ttaccttgcc tatggcgcac caagcaatat aaataaaggt gaatatgaac
     1381 ccaatactta tccgtatgaa atctggcgct acgataaaat aaacaatcac accaacaaga
     1441 cttttgtatt ttacagcaaa gacttgttag cacgtaattt tacacttttg cacagcgata
     1501 tgccgggcga gccttacgaa gcagcatgga atatgatttt gcatgatcgc agcattaaag
     1561 ctaaaaatat tgatgatacc ggtcaggatt tgaaaggtgt ttattcaggt gatagaacta
     1621 atgataattt taatgccaag taattttagg tgacctttct ctttctgaag tttgtaattc
     1681 gatgtattgc ttttcttgat ttaagaatac tatatctctt agctgatggt attgccttct
     1741 gttttaaata tattttccca tatcgcaaaa aggtaatcct tcaaaatctt tataactcct
     1801 ttcctgaaaa aagtgaacag gagatagaag aaattgctaa gcagttttat aactactttg
     1861 ccaacctgat tgtagagacc ataaaaatgt cgagcatatc aaaagaagaa ttgctcagac
     1921 gctggcgttt tacaaatgca gaagttcttc atagctatta tgaaaaaaac cgtagtatca
     1981 ttgccgtttg tggtcacttc aataattggg agttaggagc attggcactt tctgtaatag
     2041 ataaacatca gatgcttggt gtttacaaac cattgaccaa caaatcgtgg gatgaatatt
     2101 ttagaaccat tcgttcgcga ttcggtatga taccggttcc gatgaaatca atattacgcg
     2161 aattgattaa acgtaaaaat gagccaacca taactacgct tgctgccgat cagacaccac
     2221 accgttctga aataaattat cggacaactt ttttaaatca ggatacagca gtatttctgg
     2281 gcgcagaaaa actttgtaaa caaaccggtc atgtacttat atatttttcg atgcaccaaa
     2341 ttcgtagagg ttattatgat attaccatca ttccaataag tgatgaccca aaaaatactg
     2401 ctgaatacga actcactcac aaacatttaa gattattaga gaacgacata catgcacaac
     2461 ccggtaactg gctttggagt caccgcagat ggaaatatta aataacaatc aacaatgaac
     2521 gtattaaaac aattcagatt aattgcaatt gcagaaggca tctccttttt agttttactt
     2581 tttattgcca tgccgttgaa gtatggcgca gatatgcccc tagcagtaaa atatacaggc
     2641 tgggtgcatg gacttttatt tgtgttttat gtttattcgc tgcttcaggt gtggcagcaa
     2701 tataaatgga atttaaaaat gatgtcgtta gccatgctgg catcggtatt gccttttgga
     2761 actttttatt ttgagaagaa atatctgaat aacatataat gccctttaga tttcatttct
     2821 ttgcagcctg atgaaagctg ctgttgtcat attaaattat aatggtgcag cattactgca
     2881 acaatttctg ccttcggtaa ttgcaaacac accgtcctgg tgttctgtta tagttgcaga
     2941 caatgcctct accgacaatt ccttgtcggt aatgaaaaag cattttcctg atgtacgctg
     3001 tattgaaatg caacaaaaca ctggctttgc aggaggttac aacaatgcat tgcagcatgt
     3061 agatgcagaa tattttattt tactcaattc agatattgaa gtaactccac aatggattga
     3121 gcctattatt gaatttatgg acaaacatgc agatgttgca gcttgtcagc caaaaatatt
     3181 agcctatcac cggaaagaat attttgaaca tgcaggtgcc tgtggcggat atattgatta
     3241 tttgggttat ccgttttgca gaggaagaat ttttaattca ttagaaaaag acgaaaacca
     3301 atattcagat aacaaagaaa ttttttgggc aacaggtgct gctatgttta tcagaagcca
     3361 tgtttttaaa tcagcaggag gttttgacac tgactttttt gcacacatgg aagagattga
     3421 tctttgctgg cgtatgaagc atagtggtca taaactgtat tgtattcctg catcaagagt
     3481 atatcatgtt ggtggtggaa cattacataa aagtaacccg aaaaagacct atctcaattt
     3541 cagaaataat ctgatgatgc tgttaaaaaa tttaccttca ggcaatagat tttctgtatt
     3601 taccaaacga atgtatcttg ataaactggc tttcattaaa ttttttattt caggaaattt
     3661 taaagatgct tgggctgttt atagggctta tttttatgtg atacttcatt ttaaaacttt
     3721 aaagaaaaaa cgtgtagaaa ttaaaaatat caacaccaat gtttcctgta tatttgaaaa
     3781 aagcattgtt gcagaacatt ttttcagagg aaaaaagaag tttacagaat taaatcaaga
     3841 ttgtttttca taataattct taattgttaa tcaatgaaaa taatttgtgt tggtcgaaac
     3901 tatgctgcac atgcagcaga gctaaaaaat ccgttgcatt cagagcctgt catttttatg
     3961 aaaccagaaa cggcacaatt gctgccacga atgccatttt ttattcctga cttcagcacc
     4021 gacattcatc atgaagtgga attggttgta aaaatcaacc gtacaggaaa gcacattcag
     4081 gagcagtttg caaacaccta ttatgatgaa ataggcatag gaatagattt tactgctcgc
     4141 gacaaacaat cgcaattaaa agaaaaaggt ctgccctggg agttagccaa agcatttgat
     4201 ggcagcgcac ctacaggtaa gttcctcaat aaatcattat tcaaatcggt tgaggatatt
     4261 aatttccgac ttgaaaaaaa tggaaatatt gtgcagcaag gcaacagtag caatatgctt
     4321 tttaatattg ataaaataat tgcatttgtt tcatcctata tcacgcttaa aaaaggtgat
     4381 ttgattttta cgggtactcc cgaaggtgtt ggcaaggtac aggcaaacga tgtattggat
     4441 gcatttattg aagatcaaca tcttttgact gttaaaataa aatagttaaa aaaataatta
     4501 ttcctcatca gaacattagg tcaatacgct aattttaccc ttattaatat ttaaatttac
     4561 gcccgcaaaa atcacttttc aaacatgctt cagcaacagc aaaaggaaga aataaaaagc
     4621 ctgctccggg aggccgacct tgcaggttgc aaagcatatc tggagaattg gttgcccgca
     4681 gagttggtcg aacttattga tgagtcgcca aacgaacatc gcgttttaat ttttaaggct
     4741 ataaatcgtg aacaggcttt tgaaacctat gaatcattag acctgaatct gcagatttct
     4801 ttactcgaca ttttacctcc gcgtcaggta cagcttattc tcaatgacat gagtcccgat
     4861 tcgcgtacag cattgctcga agatattgat aacgacctgc tgaaccgctt gctgaaactg
     4921 ctcacaccaa aggagcgtgc cattgcactt tctctgcttg gctatcctga aaattctatc
     4981 ggtcgtttga tgacacccga ttacattgcc ataagaaaag aatggagcgt acaatatgcg
     5041 ctcgactaca ttcgtgaaaa cggtgaaaac tctgaaacgc tgaacatcat ttatattgtt
     5101 gatgacaaag gacatctgat tgacgatatt aaagtagccg aaatattatt agccgacctg
     5161 catattaaaa ttgaagagct gattgacgga aaatatactg cactttctgt tactgatgat
     5221 gagaaagttg ccatcagcga atttaaaaaa cacaaccgtg tggccttgcc tgtggttgac
     5281 acttcgcaaa tgctgttagg gattattacc gttgatgatg ttttacaact tgcagagcag
     5341 gaatattctg aagacatcca gaaacttggt gccgttgaag cgttggaaga gccatatatg
     5401 gaagtgccca tgtatcgtct gttgcaaaaa cgtgctccct ggctgatagt actttttatc
     5461 ggtgaaatgt ttacggcttc ggcaatgagt ttttttgagc atgaaatcca aagagctgta
     5521 gtgcttgcat tatttattcc gctcattgtc agcagcggtg gcaatgcagg ctcacaagca
     5581 gctacactta ttatccgtgc attggcgctt ggcgaagttg gcatgaaaga ctggtggcgg
     5641 gtaatgcgca gagaaataat ttccggattg atgcttggac tcattctggg aagtattgga
     5701 tttttacgtg ttgccgcatg ggcaatgttt acagatattt acggaccaca ttggctgctc
     5761 attggcatta ccattggcat aacgttaatt ggtgtctgtt tatggggaac tgttatgggc
     5821 tctatgttgc ccattgtttt aaaacgcttt ggtgccgacc ctgcaacctc ttctgcacca
     5881 ttcattgcaa ctttggttga cgttaccgga ttggtaatct atttttctat tgctgcaaca
     5941 atactctcgg gaacgctgct ttaaatatta atttattatt ggtataagcc ctcttacacc
     6001 catatcaaca acagtgtcgc cattagcagg ttcatacact ccgggggtgc tgtgttctac
     6061 ccattcaaag ctcttgttag tagagataga tactgtaatc gttttatctg atgtttcatt
     6121 tccggtaact acaaatggtg cagaaaagac acctgtaacc acacatgacc ctgcagggat
     6181 tggcgaagtt gatgccagcg gattaggaac agttgttgca ccggcaggag cctgtccgct
     6241 gatgacagga ggaagaaaac ccgggaaagg ataattttga tttaaggttt caaatgccca
     6301 gtaaccttgc ttacgattag catttaatgt gacggtgctg tcctttattt taaatgagtt
     6361 aataaaagag ttgaatccaa taaaagaagc aacagtacct ttaaggttgg gataaacagt
     6421 tccaagaata ttggcaccga aggtgatgtc ataattctga tacgctaatg aaacacgtaa
     6481 gtactgatat gtgccgggag caatgttttt aaacggcact ctgaaaaact cttcattgtt
     6541 acctgcaagt accgactctg aaaaaacaat agcattgtcg ccaccttgtg tggtttcact
     6601 atttctgtat agcactgccc cactacctaa ctgcgtaaat gccgttggtg caagttcaat
     6661 atagtgcgaa ctcatggaat taaatactgg cgataatgca ccgtgattag caggtagcgt
     6721 tgctgcattg ccaaaattat cctgacgctg ttgtgtggag tcaaaacgaa acttaaatat
     6781 caactgcgct tcgttatcat catctttctt cttgcatgat gtagttatga cgaatgccaa
     6841 cagaataaat aatacatttt tcatttgttt gatttttaaa ttctatgctg caataataat
     6901 catatcctat aataggaaac gttgtagttg atgaataatt gtactttaga agtgataagg
     6961 tgattatgca atgaagtaat aatttaagtc ctctataccc gtttagttgt attttggaat
     7021 tccaagaaaa ataaaacaaa taagaatggc ttttacactt gattgaagat gataaatttg
     7081 agttaaatat actgcatttt ttttaaaaag caaatatttt ttattcattt acatcctaac
     7141 tccaaattca aactgcaaaa tgtattgctg tttaagtttc tgtcaaaaga aactttgctt
     7201 ccacaatgaa acttacattc aagaaagtat tttgtcaatt atcaattaca cacctatgga
     7261 aatatttaat tacaaattga aaataatctt tacacctcca acaaatcttt gtctttaatt
     7321 gcaagcagtg ggcaaacatc gcaggccata cgaccttcct gataaaacca acggcatctt
     7381 tcgcgaatac tgcattgcgg taaaatggca tcttttttta aggtcaagtc ttcaacattt
     7441 ttaagaacct tacccacaca gccacaagct gctcctgtcc agtgtttgca tccagtttcc
     7501 agacaaacat tgctgaaacg aaatcgcttt tcaggattgc tgactgcagt cataacatca
     7561 aaaaggtctt cgtccaccat gatgttttct tttaaaaaat caaatacatc attatcaatt
     7621 tttacaccta tgagttttgc tccgggttcg catttggtga tggggcacat tttcaactct
     7681 ttcatgtctg taatttttgc ggttttataa tatttacttt ttttcacttt taaaattttc
     7741 tgaccccggc attcgttaac accgggaatc agattttttc aattgggtta gcctcagggc
     7801 gatttccaaa tccgagttga ggatatgaca tcaaagtaaa atacaattaa ttttacaacc
     7861 taaaggtttt gtatcaaatg cagatgcggt tgtacgaggg aaataaagct gctaaatagg
     7921 cttcaatctc aataaaaaat cttcttttct ccctctggct atctctatgg tattgccatt
     7981 tgacatgatg acttcaccac cattattgtg gcttacagat tttacgtgcg ctaagtttac
     8041 catatttgac ttatgtaccc gaaaaaagtc gaaatcggtg agtgcttttt cataaacacc
     8101 taattgttta gtgaccaaac gctctgtatt gtctgtcaga aaaaaacggg aataattgcc
     8161 ttcgcctctg atgtgaataa cctgctttaa tttgagcaca tcaaagcctg aaaattttgg
     8221 aagagcaatg tgcatttcgt ccttttggct gatgttatgg ttgtgcaata aaacctgaag
     8281 catttcagcc gaccttttac gttctgcctc tacctcaaac ttttttatgg cacgctgaag
     8341 gtcttcaggt gtaacaggct ttaccaaata atcaacagcc gctacttcaa atgcacggac
     8401 tgcatattta tcaaaacctg ttataaaaac aacaccaaag tcaatctttt caaacctttg
     8461 caaaaggtca aatccggtat agggcggcat gtcaatatca ctgaaaacca aatcgggctt
     8521 tgtgctgcgt atgagtgttt ctgcctccgg tacagagctg gcagtgccca gaatctttat
     8581 ctgcggaaag taattttcta cacagtcttt tataagatta atctggttga cttcatcatc
     8641 aactagcaca aggtttatca tggcttaaaa gtagcggaaa tccgacaaaa aaactcaggt
     8701 tgcaaaaaat attttgcaac ctgagtacct aacctaagcc tatgaaacaa tacaaccaat
     8761 aaacagcaca gcaccaattt tgttttagcc gctcacttat taactgaatg tgaatgacat
     8821 aatcatttta catacattcg catctctaaa aaatcaaaca aaatatatgc ttaaacgcta
     8881 catcatgctt tcccttttat ggattgcgcc atggattaca tcagccacga acaacacaac
     8941 aggtcatttt acacttcctg caggtatttc atctgaagac taccaacaag gaaaggttat
     9001 ctttaaagtt aaggactctt accggtcact ttgtactgct acaggcataa acatacaacc
     9061 actgaatgtg gctttaaata acataggctg tacggctgtt tctaaaatgt ttcccggcaa
     9121 agagaagcca actactgaaa gaaatgctat gcatcagcag tatgccgatt taagtttaat
     9181 ttatgtggcg cagattaatc cttctgcttc catagagaaa gccatcaatg caatgcttgc
     9241 aacaggtgtt ttaatttatg ctgaaccatc ttacatctat aaaacaaact ttacacccaa
     9301 tgacgggcag ataggctcgc aatggtttct tacaaaaatt caggctttta gtgcatggga
     9361 tactcaacaa ggcgacacct ctgtggttat aggcattgtt gactcaggca cagactggga
     9421 tcacccggat cttcagaaca atattaaata caactatgca gacccaatta atggtgtgga
     9481 tgatgacaat gacggataca ttgacaacta ccgtggctgg gacatgagca tgaacgacaa
     9541 caatccaatg gtggatatga gtgatcatgg ctcgcatgta tcaggatgtg ctgctgctac
     9601 aactaataat ggaacaggtg ttgcctcgcc cggatttaat tgtaagttct taccggtgaa
     9661 gtgcgccaat gccacctccg ttactacaat tgacagaggt tatgaaggca tagtttatgc
     9721 tgccgatcat ggatgccaga ttatcaattg ctcatggggt ggcagtgcag gtggcagtgt
     9781 tggacaagat gctattacct atgcaacagt aaataaaggt gccttggttg tttgttctgc
     9841 aggaaacacc tacaccgata ttgcacaata tcctgctgca tataattatg catttagtgt
     9901 tgctgcaaca aactccagcg atgaaaaatc atctttttct acctataatt attctgtaga
     9961 tatttctgca cctggctcaa atattttctc taccctatat aacaacagtt atgcttcgtt
    10021 agatggaact tcaatgtcgg caccaattac ctcaggttgc gcagccatag taaaatcaca
    10081 attcccgacc tataacaaca tgcaggtggc agagcaatta cgaatgactg ccgataatat
    10141 ttatggtgtt tcgggtaaca gtggttttgt tgataaatta ggtaccggac gtgtgaatct
    10201 ttataatgca gttacacaaa gccctaaagg agtgcgtatg gataatatca gcctgtctga
    10261 taacaatgac cttgcatttg ttgccggtga taccttacga attacaggtg atgtgctaaa
    10321 ccttcttgca cctctttcaa atcttacagt tacactttca acagtatcaa gtagtgtaac
    10381 tattttgaat aacaccatca atcccggtgc tatggcaact cttgatgtgg tgaacactgc
    10441 caccacacca tttttagtta aaataaaacc tacagctccg gtaaattcaa atgttgtgtt
    10501 taaactttct tttactgatg gcaaccttat cgttaaccaa tattttactg ttgtagtaaa
    10561 tgtagattac atcaatgtta acatcaatga agtggcaaca accataacca gcaaaggacg
    10621 tttgttttat tatggcatca atcagacatc aggattaggt tttgatttta atggtgataa
    10681 tttggtttat gatggtggat tgatgattgg caacaacaat cttgtttctg acaactgtcg
    10741 tgacggttct aattttgatg atgattttca aaaacaaatt gctattaaag ctataattcc
    10801 ctctgtaaaa tcagaatttg acctgacaac aaattttaac gacaataatg tcactgctgc
    10861 aaacagactg aaagttttag tcagacataa cacatacgca tggagttcac cgggcaactc
    10921 gcgttttatc attgttgaat atcatattaa aaatacaggc accaatgcat tgagtaccct
    10981 ctatgccggt atttgtgccg attgggatat tcaaacctat gccaacaata agtctgatgt
    11041 tgatgctgta aataaaatgg ggtattcgtg gtgtacagat aatggcggac tttatgcagg
    11101 agtaaaattg ttgtcagcaa caccatttaa ttcttatgct attgacaatg taaccggtgg
    11161 tgccggtggt gttgatgcaa caagtgattt taccaattca gaaaaatata ctacactaag
    11221 cacaagtcgt gcgcaggcag gaaacacact tcctgcaggt aatgacgtga ttcaggtggt
    11281 aagttcagga ccatttaatc ttgccggtgg tgattcaata acagttgcct ttgcattgat
    11341 agcaggtgac agccttgccg atattcagca aagtgctgct aatgcacaaa taaaatatga
    11401 tggtatttta acttctgttc aggaaaatat tgctcatgga tcagaaacac aattactgaa
    11461 tccctaccct aatcctaaca gtggaaatga attaaacata cctttttata ttgcaaaaaa
    11521 tcaaataaat tctgtaaata tcagtctgac agatatgaca ggaagaacag ttgttgatct
    11581 tcaaaacatc cagacaattt caggaatgaa tatacagaca gttaacatgg cagggttaag
    11641 caatggtatt tatctgatta aattagaaag tgccggcaaa atatacaccg gaaaaatttc
    11701 tgtacagcat taatttttaa attaaccata attaatattt caaaccacac agacaatggt
    11761 cgttctgtgt ggtttgattt ttctacctta gccaagagat taatacaaat tcaaaatcat
    11821 taattactgt atgatgaaaa agaaatcaat tttaatactt acaatcatga gtatagcttc
    11881 tgcttctttt gctgaaaaca aagatttaaa agaacaatcg gctaatccat tattacaacc
    11941 ctggacaggg ccatacaatg gtgttccggc atttgataaa gttaaagaag aatattttat
    12001 tcctgctttt gaagcggctt ttgcagagaa aaaagcagag attcagaaaa tagcttcaaa
    12061 caaagaagct ccaaatttta aaaataccat tgaagcaatg gaaaaagcag gagctacttt
    12121 aaatagagta agagctattt atgatatatg gtcatcgaat ctgaatacac ctgtgatgac
    12181 tgctattcag gaaaaaattg aaccacagat tgcagcattt aacgatgaag ttattcaaaa
    12241 taaaccgctg tttttaagaa tagaaaaagt gtgtagcgat actgttggga aaaaatatag
    12301 tgtagaacaa catcagttgt taaaacgtta ttacaacaat tttgtccttg caggtgctaa
    12361 acttaatgat gctgataaag ctaaggtggc taatatcaat tcacgattgg ccactttgtt
    12421 tactcaattt tctaaaaatc aattagcaga tgagaatgaa aaatatatag aaattaaaaa
    12481 tgaatccgat ttggcaggat tacctgatga cataaaatca gcgtatgcaa aaaatgcaaa
    12541 agagcgtggt aaatcaacat ggactgttgc caatacacgt tctgctgttg agcctttttt
    12601 aactttttca aacaatcgcg aattacgtaa acaagtttgg acaatgttta taaaccgtgg
    12661 cgacaatggc gatgccaacg ataacaacaa gattattcct gaaattttaa aattacgtta
    12721 tgaaagagca aagctgatgg gatttgcaac atttgcacat cagaaacttt cagacaaaat
    12781 ggctaaaaca cctgaagcta ctatgaaatt gatggaatct gtttggcctg ctgctattgc
    12841 cagagtaaaa gaagaagttg ccgacatgca ggcaattgcc gataaagaaa atgcaggaat
    12901 taaaattgag ccatgggatt atcgctacta cgcagagcaa gtaagaaaat caaaatatga
    12961 tctcgatcag aatgaggtga aacaatatct tcagttggag aaacttagag aaggaatgtt
    13021 ttttgttgca ggagaattat tcaatctttc atttactcag gttcatgacg ttcctgtttt
    13081 tcatcctgat gttacagtat ataaagttga agataagaca acacataagt taaaggggat
    13141 ctggtatttt gatccctatg cacgtacagg taaacgttcc ggtgcatgga tgacagccta
    13201 tcgcgaacag gaaaaactga gtggaaatgt tacaactatt gtatccaaca attgcaatta
    13261 cataaaaggt tccggtaatg atcccgtact tatttcatgg gatgatgcca ctactctgtt
    13321 tcatgaattc ggacatgcat tgcatggttt gtgttctgat gttacttatc cgacaatttc
    13381 aggaaccaat gtggccagag attatgttga gtttccatca caagtacttg aacgttggtt
    13441 gccaacatca gaggtgttgc agaagtatgc aataaatgct aaaaccggac aacctcttcc
    13501 acaaagtctg ctcgaaaaac tcaacaaatc ttcaaaattc aatcagggat ttgaaacaac
    13561 agaatatctg tcgagtgcat tggtagatat gaaaatacat cttgcaggtg ataaagtaac
    13621 tgatcctgat aaatttgaaa aagaaacctt agctgcactg aatatgcctt cggaaattgt
    13681 gatgcgtcac cgactgccac aatttgggca tattttttca agtgatgaat atgcagcggg
    13741 ctattacagt tatctatggt cagatgtaat cagcgctgat gcaactgaag catttacaga
    13801 agcaggtagt ttatatgata aaaagactgc cgaaaaactt tatcgtaatg tgtttagtgt
    13861 tggtgcttca attgacgaaa gtataggcta tcgtaatttc agaggtcacg accctgacgt
    13921 taaagcactt atgcgcagca gaggctttcc tgaaaaataa gccttggagg gcttattacc
    13981 ataactattt tgatgatttt ccgtatgatt aaatactttt gtttagttta accttacggg
    14041 atgtgatcaa aggcaacttc aggtattttc tacttattct ttctttggca atggtatttt
    14101 ccgccattgt attagagtgg aatatgcaca aacgtgatct tgactttatc aggataacag
    14161 tttttgcaaa aaagacttta catgaagaag aaaaccgggc aaacaaatat gttgaatggc
    14221 ttggtaatat gctttcgcgc caaacaagtt attctgccat tgacgacagt ctgttgctta
    14281 cttcgcaaaa acctgttagt gctttctaca gctttgtata tctcaacgac tcattaattt
    14341 attgggataa taatgctgta agtttctc
//
LOCUS       DAOOOL010000063        14343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_364_length_14343_cov_191.370381, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000063 DAOOOL010000000
VERSION     DAOOOL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14343
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_14343_cov_191.370381"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..949
                     /locus_tag="PKU82_06595"
     CDS             56..949
                     /locus_tag="PKU82_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOZ82579.1"
                     /translation="MDATELIKKVRKIEIKTKGLSNQIFSGEYHSAFKGRGMAFSEVR
                     EYIPGDDIRSIDWNVTARLNHPYVKVFEEERELNVMLVVDVSASGNFGTQKQFKRELI
                     AEICAVLSFSAIQNNDKIGLILFSDKIEKFIPPNKGRSHILRIIRELINFEAESKGTS
                     ISAGLQFLSNAIKKKSIAFLISDFYDSAVGKPKSMLEDSLKIANRKHDLVVLKINDLR
                     ESELPDIGLINIYDAETGREQLIDTSSAVVRKNYQNQRQMRNRQINGLLSKSGIDKIE
                     LFTGQNYIQPLMKLFKNRVKK"
     gene            946..1911
                     /locus_tag="PKU82_06600"
     CDS             946..1911
                     /locus_tag="PKU82_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82580.1"
                     /translation="MKLFIKKIPALLISILLSANCVFAQSVTAVLQLDTNRIVAGQQI
                     TAKLRFTAPADYQTKWVAIPDTFNGIDVISRLPIDTLPSANKSLITREQKFTLTAFDS
                     GFFVITPFVFSYKKTGDTTNYTVETQAQLLTSNLMPVDTSKAIHDIKGPMDVGITWQE
                     VAMYSSIILLLIAIIWYVVYRLRKKKPQTVIKVPDAPKRPAHEIAIEQLEHLQQEKLW
                     QQGNFKMYYTQLSDIMRAYISNRWNFDAMESTTDEIMHSNTALQLSNDNFNRLKSTLT
                     LSDLAKFAKYTPLSNENEQAMTDAFIFVNETKVVLTTKPQEQVKP"
     gene            1933..2916
                     /locus_tag="PKU82_06605"
     CDS             1933..2916
                     /locus_tag="PKU82_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ82581.1"
                     /translation="MQFAYPQFFWLLLLIPAMLLWQWKVTSAKKVRLKLSSDDGFQNY
                     QPSFRQRLVFMPFVFRLLSVICIIIALARPQSSSQAQQVSTEGISIVLAMDISGSMLA
                     EDFKPNRIEAAKKVAIDFIDHRPNDLIGLVIFSGESFTQCPLTSDHAVLKNLMSKIES
                     GMLTDGTAIGEGLATAVNRLKDAKTKSKVIILLTDGVNNAGAIAPLTAGEIAKTFGIR
                     VYTIGVGTVGMAPYPFKTVFGIQYQNVPVQIDEEICKQISDATDGKYFRATNNKTLKE
                     IYSEIDKLEKTKVEVTEFRRHSEEYFNYALAAGIFFALELLLKYTWLRKLT"
     gene            2940..3992
                     /locus_tag="PKU82_06610"
     CDS             2940..3992
                     /locus_tag="PKU82_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOZ82582.1"
                     /translation="MFRFSHSFILYCLALIPLFIILYISLVNWRKKSLKKFGDMHLVR
                     FLTPGKAYTKPLIKFILLMTAFLFLVIGIAGPQVGTKLETVKRKGVDVIIALDVSNSM
                     NAEDIKPSRLERAKQSISRLIDQMQGDRIGLIVFAGEAYTQLPITTDYGAAKLFLSTV
                     NTNIVPTQGTAIGKAIDLAVSSFGNEDSSKKNKAIIVITDGENHEDDAVAAAKEAYKN
                     NITVHTIGMGSVNGAPIPVYTNGQRTGFMQDNNGQTVITKIDEQTLAEIAQAGNGKFI
                     RATNSDDGLSIIMKEINAMQKNEFGSKMFTDYADQFQYLLGAALFFLILEFLISEKRS
                     RWMEELNLFGDGDEKK"
     gene            3979..4701
                     /locus_tag="PKU82_06615"
     CDS             3979..4701
                     /locus_tag="PKU82_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82583.1"
                     /translation="MKKSNILILFFISMSFVAIAQNEKPLVREGNKEYKNGKFDQAES
                     SYRKAIEKNGNSVEGLYNLGNALYKQNKFDEAAGVYQNLNNEKLSTDAKAKSLYNLGN
                     TLMKSEKYQEGANAYKEALKLSPNDKDALYNYSYALAKLKQQQQQQKQNQDNKKDQKN
                     QQKKQQDKQQQKQDQQKQQQDQQKEQDQQKQDQKKEQAQQPKISKEDAERMLEALKND
                     EMNLQKKLAKKEGTRVNIEKQW"
     gene            4695..6560
                     /locus_tag="PKU82_06620"
     CDS             4695..6560
                     /locus_tag="PKU82_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOZ82584.1"
                     /translation="MVETKAIKIKHKSLFAGLVLLLLFFTTNIFAAEITFIASVNKNP
                     VGTNEQFSITYTLNTQGSNFQAPTFRDFNVLSGPNQSTSMQFINGNMSQSVSFTYYLT
                     ANSEGTFRIDPATITVNGGKVKSNTLTINVVKSQKAQSGAQQQKSNDADAGISSNSVF
                     LRVSVNKSSVYRGEALLATYKLYTKVNLVNYSIDKLPALNGFWSQELKMPQQLELTTE
                     NYNGEMYRVGIVKKVLLFPQQSGTLTIEPMEGSCIARIQTQRKRSNNPFDIFNDPFFN
                     DPFFGSAQDVKVNVKSLPVKITVKDLPQENDNAFKGAIGSFSLQATTDNDKVKANDAI
                     NLKIKISGKGNLKLIDAPEVEVPTDFEKYDPKVSDNTNISESGVSGSKTFEYLLIPRH
                     EGEYTIDPIKFTYFDLDKKQYEQLQSSAFKITVKGISGDASAAQSVATSQNKTDIKLL
                     GKDISFIKTGKLTTKPATTFYLSGLYYLLVAIPFAALILLILYIRKRNRLAGDAEYMR
                     SSRATKMAHKRLSIAKKMIDAKKDDAFYEEISKAIYGYASDKLNINFSEMTKENLRQK
                     LINRNIPDALTERIIATIDLCELARYAPLTQNNNTQSIYTEAAAIITELENYLNK"
     gene            6564..7325
                     /locus_tag="PKU82_06625"
     CDS             6564..7325
                     /locus_tag="PKU82_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82585.1"
                     /translation="MKARYIFILLCLLLSQNIFADSKHDIVFNEANNLYLKQSYFQAI
                     EKYEQLVADNTISFEVYYNLGNAYYKTGNFTKAILNYERAKKIKPDDADVNLNLAIAN
                     QKTIDKIEPTPKVFYNQWWDNYIASSTADSSAVIMLFFLWLFIAAIAIMIIIKSASLK
                     KLCFIISLILACCTLFFFITTQTRQNINDSSVEALIISQSAYIKSAPDDKGTNLFQLH
                     EGTKINIVDELSSWKKIRIPNGNIGWIKSKDLETI"
     gene            complement(7326..9221)
                     /gene="asnB"
                     /locus_tag="PKU82_06630"
     CDS             complement(7326..9221)
                     /gene="asnB"
                     /locus_tag="PKU82_06630"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ82586.1"
                     /translation="MCGIAGSFSVSNFFSEQDFRKASAAIKHRGPDGDGFYSDNFVRL
                     AHRRLSIIDLDNRASQPMYSSCMRYVMVYNGEVYNYVELRKEIESEYKDSQLSDFKTT
                     SDTEVILRLFEIKGVDFVKDLNGMFAIAIYDTQANKLFLFRDRFGIKPLYYYHDSSGN
                     FCFASELKAILAIDSIERKVNSQAVAYFLHTGFVPAPLTMYEKVFKLESGNFLTIDRN
                     GLEIHPFVMNRKCSQREIEHDEKKAIQNIEKLLIQSVKIQLRSDVPYGVFLSGGVDSS
                     LLTAIAARNVDEKLNTFSIGFAEQSHNESEHARVIAKHIGTNHHEFVVSVKDAIDLIE
                     TFFEVYDEPYTDTSGIPTMLVSKLASKHVKVVLSGEGGDELFFGYGTHNWAKRLNAFP
                     FSVFRSPIRTILTCGNSRSKRVAQLLDFNEHSDFLPEHIYSQEQYLFSNKEIVELVSD
                     ELRKETLKNIAQRNEELEKLFNINDSCLPEERQANYELKFPFQDDLLTKVDRATMFHS
                     IEARVPFLDNNFAEYVFSVSSDIKIKNGINKYPLKKILANYVPEELFMRPKKGFSIPL
                     AKWLKADWKYLLNRYLDEKTIRHAGLVKFEVVKKLIQRFLSGEDYLYNRIWCLIVLHR
                     WYERQQW"
     gene            complement(9221..10114)
                     /locus_tag="PKU82_06635"
     CDS             complement(9221..10114)
                     /locus_tag="PKU82_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ82587.1"
                     /translation="MLTDKPPFFSVIMPVYNRADLVKDSIVSVLNQSFKDFELICIDD
                     GSTDNTLQVLKEVSKIDRRIKLISHQNKGRCAARNNGINAASGDWIAFLDSDDYYLEN
                     HLSTMYKLVQEFPSFSGIATELVVESKIRDYANCNYYRDQRVFSLSDFAEANPISLNQ
                     FCYKKSHYPELLFPDINILTAEDLLFFRMFSFRYKILKSGIITNFVNSHVNRSVNLIS
                     TEQFVYWNKFATNYFIDNFSLPTNIKAKMQQYLQLFVSNVYLSSNYTRKGLTELMKVL
                     KYPSTYTDMHFYKAIAKIFIS"
     gene            complement(10104..10976)
                     /locus_tag="PKU82_06640"
     CDS             complement(10104..10976)
                     /locus_tag="PKU82_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82588.1"
                     /translation="MIEVIANEHTFHVFMFYGDETKNFVKQVSFVKAQYEKNIVLHVA
                     HINSDVEQLSSRIDELKCNFKVVFHVVQKSPFEKVSELVGILRTVEAGWICLLKPGDE
                     WLPYHLSAFRDAMLHFKDFQAYLTLNYLRGYSTIKSVGPEDNIINVKKIELKEQLSLS
                     QLMIHSSLLKSNVFPDFKVDSHFDTLFELILVCSSGMVRINRFTNICTLRYPIHTKAY
                     FDQQTLIVCYLIRNIVKDHRLRFYLLFINWQIGFMGLLKAFRLIGAGVFFKEMLCYYV
                     GLKRGVKKGQIYVD"
     gene            complement(10969..12087)
                     /locus_tag="PKU82_06645"
     CDS             complement(10969..12087)
                     /locus_tag="PKU82_06645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ82589.1"
                     /translation="MKEKVCFILSFHDRAVQYEWIIERALKQNLNLHFIFLNPAQWHI
                     EEYVRMRKIPCLFIKYHSKRDIPRAVALIYQYLVKEKITTVHTHLFDANLAGLTAAWL
                     ANVKKRIYTRHHSDFHHRFYPKAVRYDRYCNRLATDIVAISQNVYDILIEKEKVKPEK
                     IHLIHHGFEMELFSKVPTSEVMALREKYNTKDRFPVIGMISRYEESKGIQHVIPAFKQ
                     LLKKYPNALLVLANSHGSYSKQIKKCLEQIPIENFREIRFEYNLFALYKLFDVFVHVP
                     EYKEYEAFGQVYVEALAAGIPSVFTVSGIAAEFIKNNENALVCNYKNSDDIYNAVVKL
                     LTDDGLRNRLVENGLKVVQTKFNMDRYFSHLKKLYGYD"
     gene            complement(12084..13355)
                     /locus_tag="PKU82_06650"
     CDS             complement(12084..13355)
                     /locus_tag="PKU82_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82590.1"
                     /translation="MKKVLLITYYFDPGNAVATPRIMSWANEFHQDGIELTVVTRHFK
                     GDEWHWLDFIKETTEPIKVVKNERFSVHYLPYQSGKPALVNKNKLYSKFYHLKTFLQG
                     NFNTEINAYKAFGGYCRELLRKNPQDLILVSAPPHNLVRLAGELGKEFDLPFVVDFRD
                     IWNNLITGKNELSMRKSFSNFIEEKYIGKWLKEARLVTTVSQALVEQVKRIYNGKCIE
                     LTNGYEMKLFNQVNSTNPPSKRFIFASIGTIYPIQNISIMIEGLKIFYDKLRDKSQFE
                     IKFIGLGAIESVAQYVKSELNYLKPIITQKLSRKEVFKHYIESHVLFIPAWSNHKGIY
                     STKIFEYIGSGRKILVAPGDNDVIDNIVRKSGRGYIANSAEEFASLLSEIYDLWQSGK
                     YRAEQPAEWEQLFSREKQAELLANEILKIAK"
     gene            complement(13377..14180)
                     /locus_tag="PKU82_06655"
     CDS             complement(13377..14180)
                     /locus_tag="PKU82_06655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ82591.1"
                     /translation="MKKLMLCLLAIMTACKAGKQSVETHHAQTNFMTTQSGVKIEIKE
                     KGNGIKPSPGDRITVHYTGKLQNGTKFDSSVDRGEPFVFNVGTGQVIKGWDEAFSMLQ
                     QGDKAVLTIPPDAGYGSRAVGTIPANSTLIFEVELLRVSPKIVPTPYNVQGKDTVKLP
                     SGLQYIMVKEGTGKTAVSGATVAVHYSGYLEDGSMFDSSVERGEPISFRLGEGRVIQG
                     WEQGIALLKTGSQCRLIIPYQLGYGEQGYPPVIPAKSTLIFDVELLEVN"
BASE COUNT         5007 a         2648 c         2311 g         4377 t
ORIGIN      
        1 ttaaaaacat tcaacaaatt taattcaaca gaatcaataa acaattgcca acgctttgga
       61 tgcaacagaa ctgataaaaa aagtccggaa gattgagatc aaaacaaaag gtctctcgaa
      121 ccagattttt tcgggcgaat accattcggc tttcaaaggt cgtggcatgg cttttagcga
      181 ggtaagagaa tatattccgg gtgatgatat ccgctctatt gactggaatg ttactgcacg
      241 cttgaatcat ccttatgtga aagtttttga agaagaacgc gaactgaatg taatgttggt
      301 ggtagatgtg agtgcatcag gcaactttgg tacacaaaaa caatttaaac gtgaattgat
      361 tgctgaaatt tgtgcagtgc tttctttctc tgcaatacaa aacaatgaca aaataggttt
      421 gattttattc agtgataaga ttgaaaaatt tattcctcct aacaaaggtc gttcacacat
      481 tttgcgcatc atacgtgagt taataaactt tgaagctgag agtaaaggca catcaattag
      541 tgctggactt cagtttttaa gtaatgctat aaagaaaaag agtattgcgt ttttaatttc
      601 cgacttttac gatagtgctg tcggcaagcc taaaagtatg cttgaagatt ctttaaaaat
      661 tgccaatcgt aaacatgatt tggttgtctt aaaaataaac gacttgcgcg aatcagagtt
      721 gcctgacatt ggattaatta atatttatga tgcagaaacc ggtcgcgaac aacttattga
      781 tacttcaagt gctgttgtca gaaagaatta tcagaaccag cgacaaatgc gcaatcgcca
      841 gatcaatgga ttgttaagca aaagcggcat tgataaaata gaattattta cagggcagaa
      901 ctatattcag ccattgatga aactttttaa gaacagagtt aaaaaatgaa attatttatt
      961 aaaaaaattc ctgctctgct tataagtatt ttactttccg ctaattgtgt atttgcacag
     1021 agtgtaactg ctgttcttca acttgacacc aacagaattg ttgcagggca gcaaataaca
     1081 gctaagctcc gttttactgc acctgcagac tatcaaacaa aatgggttgc cattccggat
     1141 acttttaatg gcattgatgt tatttcgcgc ttgcctattg acaccttacc ctccgccaac
     1201 aaaagtctaa ttacacgtga gcaaaaattt acacttactg cttttgattc aggatttttt
     1261 gtaataactc catttgtatt cagctataaa aaaacaggcg ataccacaaa ctatactgta
     1321 gaaacacaag cacaattact gacttcaaat ctgatgcctg ttgacacctc taaagccatt
     1381 catgatatta aaggaccaat ggatgttgga ataacctggc aagaagttgc aatgtattct
     1441 tcaataatac ttttgctaat tgcaataata tggtatgttg tctatcgcct gagaaagaaa
     1501 aaaccacaga cagttataaa agttcctgat gctccaaagc gaccagctca tgaaattgca
     1561 atagaacaat tagagcatct tcaacaagaa aaattatggc aacaaggtaa tttcaaaatg
     1621 tattacacac aactttcaga tataatgaga gcatacatca gcaaccgttg gaattttgat
     1681 gccatggaaa gtaccactga tgaaataatg catagcaaca ctgccttgca attaagcaat
     1741 gacaatttta acagactgaa aagcacatta acgttaagtg atttggcaaa atttgcaaaa
     1801 tacacaccgt tgagtaatga aaatgaacaa gcaatgacag atgctttcat ttttgtaaac
     1861 gaaaccaagg ttgttctgac cacaaaacca caagaacagg ttaagccata aaacatgagt
     1921 ttatttgata acatacaatt tgcctatcct cagttcttct ggttgctgtt gctgattccg
     1981 gcaatgttgt tgtggcagtg gaaagttact tctgcaaaaa aagtcaggct taaactttca
     2041 tctgatgatg gatttcaaaa ttatcaacca agtttcagac agcgattggt gttcatgcca
     2101 tttgtattcc gacttctcag cgttatctgc attatcatag ctcttgcacg tccacaatca
     2161 tcatcacaag cacagcaggt ttcaacagaa ggcatatcaa ttgttttggc aatggacata
     2221 tcgggaagta tgcttgcaga agattttaaa ccaaacagaa ttgaagctgc taaaaaagtg
     2281 gctattgatt ttattgatca tcgtccgaat gatttaattg gtttggtaat tttcagtggg
     2341 gaaagtttta ctcaatgtcc attaacttca gaccatgcag tattaaaaaa cttgatgtcg
     2401 aaaattgaaa gtggtatgct gacagatggc actgctattg gagaaggctt ggcaacagct
     2461 gtgaaccgtc tgaaagatgc taagacaaaa agtaaggtta ttattttact tactgacggt
     2521 gtaaataatg caggagccat tgcaccatta acagcaggtg aaatagctaa aactttcggc
     2581 atcagagtct ataccattgg cgttggcaca gttggcatgg caccctatcc gtttaaaact
     2641 gtttttggaa tacaatatca gaatgttcct gtgcagattg atgaggaaat ctgcaagcaa
     2701 atttctgatg caacagatgg aaaatatttc cgtgcaacta acaataaaac tttaaaagaa
     2761 atctattctg aaattgataa actcgaaaaa acaaaagttg aagtaactga gttcaggcgt
     2821 catagtgaag agtatttcaa ttatgctctg gcagcaggaa tattttttgc actagaacta
     2881 ctattgaaat atacctggtt aagaaaactt acataaaaga taaagattaa agcgtaacaa
     2941 tgttccgatt ttcacatagt tttattttat attgtctggc tctgataccg ctatttatca
     3001 ttctatatat aagtttggtt aactggcgaa aaaaatcact gaaaaaattt ggagatatgc
     3061 atttggtcag atttctcact ccgggaaaag catataccaa gccacttata aagtttattt
     3121 tactgatgac tgcgtttcta tttctggtaa ttggaattgc tggtcctcag gtaggcacta
     3181 aattagaaac tgttaaacgc aaaggtgttg atgtcatcat tgcgttagat gtgagtaaca
     3241 gcatgaatgc cgaggacatt aaaccaagcc gactggagcg agcgaaacaa tcaatttcaa
     3301 ggttaattga tcagatgcaa ggcgacagaa tcggcttaat agtttttgca ggtgaagctt
     3361 atacacaact acctataact acagactacg gtgctgcaaa attgtttctt tcaacagtaa
     3421 acaccaacat tgttccaacg cagggaactg ccattggaaa agctattgac ttggctgtat
     3481 catctttcgg caatgaagat tcttcgaaaa aaaacaaagc catcattgtg attactgatg
     3541 gagaaaatca tgaggatgat gcagtggccg cagctaaaga agcatataaa aacaacatta
     3601 ctgttcatac cattggcatg ggttctgtta atggtgctcc tattccggtt tatacaaatg
     3661 ggcaacgcac aggatttatg caggacaata atggtcagac tgtaatcact aaaatagatg
     3721 agcaaacctt agcagaaatt gcacaggccg gaaatggcaa atttatcagg gcaacaaata
     3781 gtgatgatgg gcttagcatt ataatgaaag aaatcaatgc tatgcaaaaa aacgaatttg
     3841 gcagtaagat gtttacagac tatgcagacc aatttcaata tcttttgggt gctgcattgt
     3901 tttttcttat cttagagttt ctgatttcag aaaaaagaag cagatggatg gaagaattaa
     3961 atttatttgg cgatggtgat gaaaaaaagt aatattctta tcttattttt tatttcaatg
     4021 agttttgtag ctattgcaca aaatgaaaaa ccattggtac gcgagggcaa taaggaatat
     4081 aagaacggaa aatttgatca ggcagaatca agctatcgta aagccatcga aaaaaatgga
     4141 aattctgtag aaggtcttta taatttaggc aatgcactgt acaaacaaaa caaatttgac
     4201 gaagctgcag gagtttatca aaacctgaac aatgaaaaac tttctactga tgccaaagct
     4261 aaatctcttt acaacctcgg taacacattg atgaaatccg aaaaatatca ggaaggtgcg
     4321 aatgcctata aagaggcttt aaagcttagt ccaaatgata aagatgcttt atataattat
     4381 tcttatgcat tagctaaatt aaaacaacaa caacagcagc aaaaacaaaa tcaggataat
     4441 aaaaaagatc aaaagaatca acaaaaaaag caacaggata agcaacaaca aaaacaagat
     4501 caacaaaagc agcaacaaga ccaacaaaaa gaacaggatc aacaaaaaca ggatcagaaa
     4561 aaagagcaag cacagcaacc aaaaatttcg aaagaagatg cagaacgtat gcttgaagca
     4621 ctgaaaaatg atgaaatgaa tttgcagaag aaattagcta agaaagaagg cacaagagtt
     4681 aatattgaaa aacaatggta gaaacaaaag ctataaagat aaaacacaag tcgttattcg
     4741 caggcttagt gcttctattg ttgtttttta caactaatat ttttgctgca gaaataactt
     4801 tcatagcatc tgtaaacaaa aaccctgttg gcaccaatga gcaattcagt ataacctata
     4861 ctttaaacac acaaggcagt aatttccaag ccccaacttt tagagatttc aatgtgttga
     4921 gcggccctaa tcaaagcacg agcatgcaat ttataaacgg aaatatgtcg cagtccgttt
     4981 cattcaccta ttatcttaca gccaacagtg aagggacttt tcgaattgac cctgcaacaa
     5041 taaccgttaa cggaggaaaa gtaaaatcta atacccttac aataaatgtt gttaaaagtc
     5101 aaaaagcaca atcgggtgct caacaacaaa aaagcaatga tgctgatgca ggcattagca
     5161 gcaatagtgt atttttaaga gtgagtgtaa acaaatcatc ggtttacaga ggagaagcac
     5221 tgttagcgac atataaatta tatactaaag taaatttggt taactatagt attgataaac
     5281 tacctgcact aaatgggttc tggtcacaag aattaaaaat gccacagcaa ttagaactca
     5341 ctacagaaaa ttacaatggt gagatgtata gagttggaat tgttaaaaaa gtattgttgt
     5401 ttccacagca atcaggcaca ttaactatag agccaatgga aggcagttgc attgcccgta
     5461 ttcaaacaca gcgcaaacgt tccaataacc cattcgacat ttttaacgac ccatttttta
     5521 acgacccgtt tttcggatcg gcacaagatg taaaagttaa tgttaaaagc ttacctgtaa
     5581 aaattacagt aaaagatctt cctcaggaaa atgacaatgc atttaaaggg gcgattggat
     5641 catttagttt gcaggcaacg actgataatg ataaagttaa agctaatgat gctattaatt
     5701 taaaaataaa aatatccggc aaaggaaatt taaaacttat tgatgcacct gaagttgaag
     5761 tgccaactga ttttgaaaag tatgatccaa aagtcagcga taatacaaat atttcagaaa
     5821 gtggtgtttc gggcagcaaa acttttgaat atttattgat accacgtcat gaaggtgaat
     5881 atacaattga cccaataaaa ttcacctatt tcgatcttga caagaaacag tatgaacagt
     5941 tacaatcatc agcatttaaa attacagtta aaggaatttc gggagatgca agtgctgcac
     6001 aatcagtagc gacatctcaa aacaagacag acattaagct tcttggcaaa gacatttcat
     6061 tcattaaaac cggaaaatta acaacaaaac cggcaacaac attttatctg agtggtttat
     6121 actatttatt ggttgcaatc ccatttgcag cacttatttt attgatattg tatatcagaa
     6181 aaagaaatag acttgccggt gatgcagaat acatgcgaag tagcagagct acaaaaatgg
     6241 cacataaacg attgtcaatt gctaaaaaga tgattgatgc aaagaaagat gacgctttct
     6301 acgaagaaat atccaaagct atatatggat atgcttctga caaactaaat attaattttt
     6361 ctgagatgac aaaagaaaat ctaaggcaaa aattaatcaa cagaaatatt cctgatgctt
     6421 taactgaaag aatcattgca acaattgact tgtgcgaact tgccagatac gctccactca
     6481 cacaaaacaa taacacacaa agcatttaca ctgaagctgc tgccatcatt acagaactcg
     6541 aaaactattt aaacaaataa aatatgaaag caagatatat attcatctta ctatgcctgt
     6601 tgctttcgca gaatattttt gccgactcaa aacatgatat agtctttaat gaagctaaca
     6661 acctttattt aaaacaaagt tattttcagg caattgaaaa atatgaacaa ctcgttgcag
     6721 acaataccat ttcatttgaa gtttattata atctgggtaa tgcatattac aagacaggca
     6781 acttcacaaa agcaatttta aattacgaaa gagcaaaaaa aattaaacct gacgatgctg
     6841 atgtcaacct caatttagcg atagctaatc aaaaaacaat tgacaaaatt gaaccaacac
     6901 caaaagtatt ttacaatcag tggtgggata attatatagc ctcctccacg gcagactcat
     6961 cggctgtcat tatgttattt tttctttggt tgtttattgc agcaatagcc atcatgatta
     7021 taattaaaag tgcttcattg aaaaaattat gttttatcat cagtttaatt ctggcttgct
     7081 gcactttgtt tttctttatc acaacacaaa cacgccaaaa cataaacgac tcatctgtgg
     7141 aagcattaat cataagccaa agcgcttata tcaaaagcgc gcctgatgat aaaggcacca
     7201 acttgttcca attacatgaa ggaacgaaaa ttaatattgt tgatgaattg agttcttgga
     7261 aaaaaataag aatccccaat gggaatattg gatggattaa gtctaaagat ctggaaacaa
     7321 tttaatcacc attgctgtcg ctcataccag cgatgaagta cgattaagca ccagatacga
     7381 ttatataaat agtcttctcc ggaaagaaat cgttgaatta acttttttac aacttcaaat
     7441 ttcactaatc ctgcatgtcg aattgttttt tcatcaagat aacgattaag caaatatttc
     7501 caatcagctt ttaaccattt tgcaagagga atagaaaatc cttttttggg tcgcataaat
     7561 aattcttcag gaacataatt ggcaagaatt ttttttagtg gatatttgtt tattccattc
     7621 ttgattttaa tgtcactgga aactgagaaa acatattcag caaaattatt gtctaaaaaa
     7681 ggcacacgtg cttctataga atgaaacata gtagctctat caactttggt tagcaaatcg
     7741 tcctgaaatg gaaattttag ctcataattt gcctgtcttt cctctggcaa acaactgtca
     7801 ttaatattaa acaacttttc caattcttca tttctttgtg caatattctt caaggtctct
     7861 ttccttaatt catcagaaac caattccact atttctttgt tactgaacaa gtattgttct
     7921 tgtgaataaa tgtgttcagg caaaaaatcg gaatgctcat taaaatccaa taattgtgca
     7981 acacgtttac tgcgtgagtt accacaagta agaatagttc ggataggact tctgaaaacg
     8041 ctaaaaggga aagcatttaa tcgcttagcc caattatggg taccatagcc gaaaaacaat
     8101 tcatcaccgc cttctcctga caacactacc tttacatgct ttgaagcaag ttttgagact
     8161 aacattgtag gaataccaga ggtatcagta taaggttcat cataaacttc aaaaaaagtt
     8221 tcaattaaat caattgcatc tttaacagaa acaacaaact catgatgatt tgttccaatg
     8281 tgttttgcaa tcactcttgc atgttctgat tcattatggc tttgttcagc aaaaccaatt
     8341 gaaaatgtat tcagtttttc atcaacgttt cttgctgcta ttgcagtaag caaacttgaa
     8401 tcaacaccac cacttaaaaa gactccataa ggaacatcgc tgcgcaattg aatctttacc
     8461 gattgtatca gaagtttttc aatgttttga attgcctttt tttcatcgtg ttcaatttct
     8521 cgttgtgaac attttctatt catcacaaac ggatgaatct ccaaaccgtt tctgtcaata
     8581 gtcaagaaat ttccgctttc aagcttaaat actttctcat acatcgttaa tggtgcagga
     8641 acaaatccag tatgaagaaa atatgctaca gcctgactat ttacttttct ttcaatacta
     8701 tcaattgcaa gaattgcttt aagttcagat gcaaaacaaa aatttcctga tgaatcatga
     8761 tagtaatata aaggttttat cccaaatcta tctctaaaca aaaataattt gttggcttgt
     8821 gtgtcataaa tggcaattgc aaacatgcca ttcaaatctt taacaaaatc aacacctttt
     8881 atctcaaaca atcttaaaat aacttctgtg tcacttgtag ttttaaaatc agacaattga
     8941 ctgtctttat attcagactc aatctcttta cgtaactcta cataattata gacctcccca
     9001 ttataaacca tcacataacg catgcaggaa gaatacatag gctgtgatgc tctattgtcg
     9061 agatcaatta ttgataacct gcgatgtgcc aatcggacaa aattatcact gtaaaatcca
     9121 tctccatctg gcccacgatg cttaatggca gcagatgcct tcctgaaatc ctgctcagaa
     9181 aaaaaattgc ttaccgaaaa actacctgct attccgcaca tcagctgata aatatttttg
     9241 ctattgcctt ataaaaatgc atatccgtat atgttgaagg gtacttcaga accttcatta
     9301 attctgttaa tccctttcgg gtataattgg atgacagata aacattactt acaaaaagct
     9361 gaagatattg ttgcattttg gctttaatat ttgttggaag agaaaaatta tcaataaaat
     9421 aatttgtagc aaatttattc caataaacaa actgttcagt ggaaatcaga tttacagacc
     9481 tattaacatg tgagtttaca aaatttgtaa tgatacctga tttcagaatt ttatatctga
     9541 aactgaacat acgaaagaac aacaaatcct cagccgtaag aatattgata tcaggaaaaa
     9601 gtagttcagg ataatgcgac tttttataac aaaactgatt taatgaaata ggattagctt
     9661 ctgcaaaatc gctcaaactg aaaactcttt gatctctata ataattacaa tttgcatagt
     9721 ctcttatttt gctttcaaca accaattcag ttgcaatgcc tgaaaaggaa ggaaactcct
     9781 gtactaattt atacattgtt gaaagatgat tctcaagata ataatcatca gagtcaagaa
     9841 aggcaatcca atcaccggaa gctgcattta ttccattgtt tcgggcagca caacgacctt
     9901 tattctgatg tgaaatcaat ttaatacgcc tatctatctt tgaaacttcc tttaaaactt
     9961 gcaatgtatt atcagttgaa ccatcatcaa tacaaattag ttcaaaatct ttaaaactct
    10021 gattaagaac agagacaatt gaatctttaa ccaaatctgc acgattgtac accggcataa
    10081 tgacagaaaa aaagggaggc ttatcagtca acataaattt gtcctttctt aacacctctt
    10141 ttaagtccta cataataaca tagcatttct ttaaaaaaaa ctccggctcc aataagtcga
    10201 aaagctttca acaagcccat gaacccaatt tgccaattga taaataaaag ataaaatcta
    10261 agtcggtgat ctttaactat atttctaatc agataacaaa caattaatgt ttgctggtcg
    10321 aaatatgctt tagtgtgtat cggatacctt aatgtacaaa tatttgtaaa tctgtttata
    10381 cgcaccatac cagaactaca aaccaatatc aactcaaaga gggtatcaaa atgagaatcc
    10441 actttaaaat cgggaaatac attactcttc aataaactgc tatgaatcat caactgtgaa
    10501 aggcttaact gctcctttaa ctcaatcttc ttaacattga ttatattatc ttcaggacct
    10561 acagatttta tagtgctata acctctcaga taatttaatg ttaaatatgc ctggaaatct
    10621 ttaaaatgta gcatagcatc acgaaatgcc gacaaatgat atggcagcca ctcatcaccc
    10681 ggcttaagaa gacaaatcca tcctgcttca acagttctta agatgccaac caattcactg
    10741 actttctcaa aaggcgattt ttgcactaca tgaaaaacaa ctttaaaatt acacttcagt
    10801 tcatcaatcc tgctacttag ctgttcaaca tcgctattaa tatgagcaac atgaagaaca
    10861 atattttttt catattgcgc ctttacaaag gaaacttgct taacaaaatt cttcgtctca
    10921 tctccataaa acatgaaaac atgaaaagta tgttcattag ctatcacctc aatcataccc
    10981 ataaagtttc ttaagatggc taaaatacct atccatattg aatttcgttt gcactacttt
    11041 caatccattt tcaactaacc tatttctaag accgtcatca gtcaacaatt taacgactgc
    11101 attataaata tcgtctgaat tcttatagtt acaaacaagt gcgttttcat tattcttgat
    11161 gaactctgct gctattccgg aaacagtaaa aacagaagga attccggctg caagtgcttc
    11221 tacatatacc tgaccaaagg cttcgtactc cttatactca ggtacgtgaa caaaaacatc
    11281 aaataattta tataatgcaa acaaattgta ctcaaatctg atttcccgaa aattttctat
    11341 tggtatctgc tccagacatt ttttaatctg ctttgaatat gaaccatgac tgtttgccaa
    11401 tacaagaagt gcattaggat actttttcaa taattgttta aaggcaggaa tgacatgctg
    11461 tattccttta ctttcttcat aacgactaat cattccaatt acaggaaacc tatctttagt
    11521 attatatttc tctcgaagtg ccattacttc cgaagtggga acttttgaaa ataattccat
    11581 ttcaaatcca tgatggatta aatgtatttt ctcaggcttc actttctctt tctcaatcaa
    11641 tatatcatac acattctgac ttatggcaac aatatccgta gccaacctgt tacaataacg
    11701 atcataacga actgcttttg gataaaatct atgatgaaaa tcggaatgat gcctcgtata
    11761 aattcttttc ttaacattgg ctaaccatgc tgctgtgagt cctgccagat tagcatcaaa
    11821 caaatgtgta tgaactgttg taattttttc ttttaccagg tattgataaa tcaacgctac
    11881 agcacgagga atatccctct ttgaatgata cttaataaac aaacatggaa tttttctcat
    11941 cctcacatat tcttcaatgt gccactgagc aggatttaga aaaataaaat gcaagttaag
    12001 attttgtttt aacgcacgtt caataatcca ttcatattgt acagcacgat catggaaaga
    12061 caatataaaa caaacttttt ctttcatttt gcaattttca aaatctcatt tgcaagaagt
    12121 tctgcttgtt tctctcttga aaataattgc tcccactcag caggttgctc agctctatat
    12181 tttccactct gccacaaatc atatatttca cttaataatg atgcaaattc ttctgcacta
    12241 ttagcgatat aaccccttcc ggatttacga actatgttat caataacatc attatctccg
    12301 ggagctacta aaatttttct acctgaacct atatattcaa aaatttttgt tgagtaaatg
    12361 cctttgtgat ttgaccaagc aggaataaaa agaacatgac tttcaatata atgcttaaat
    12421 acttcctttc tacttaattt ctgcgtaata attggtttca aataattgag ctcagactta
    12481 acatactgtg caacactttc tattgcacct aacccaataa attttatttc aaactgactt
    12541 ttgtccctta gtttatcata aaatattttc agaccttcaa tcattattga gatgttctga
    12601 ataggataaa tggttccaat gctggcaaaa ataaaacgtt ttgaaggagg atttgtactg
    12661 ttaacctggt taaacaactt catttcatat ccgttggtaa gttctataca tttaccatta
    12721 tagattcttt taacttgttc aactagtgcc tgactgactg ttgtgacaag tcttgcctct
    12781 ttcaaccact tgccaatata tttttcttct ataaaattcg aaaaggactt ccgcatactc
    12841 agttcattct ttcctgtaat aagattattc cagatgtctc tgaaatcaac aacaaaaggt
    12901 aaatcaaatt ccttgcctaa ttcacctgct aatctgacaa gattatgtgg tggtgcagaa
    12961 actaatatta aatcctgagg gttcttcctc aataactccc tacagtagcc accaaatgct
    13021 ttataagcat tgatttctgt attaaaattc ccttgcagaa atgtttttaa atgataaaat
    13081 ttactgtaaa gtttattctt atttacaagt gcaggcttgc ctgactgata aggaagatag
    13141 tgcacagaaa aacgttcatt cttaactacc ttaattggtt ctgttgtctc tttaataaaa
    13201 tcgagccaat gccattcatc acccttaaaa tgtctggtaa cgacagttaa ttcaatgcca
    13261 tcctgatgga attcatttgc ccaactcatt attcgaggtg tagctactgc atttccagga
    13321 tcaaaatagt aagttataag cagaactttc ttcacttcag catttaacaa ttgccatcag
    13381 ttgacctcaa gtaattcaac atcaaaaatg agtgttgatt ttgcaggaat taccggtgga
    13441 taaccctgct ccccataacc aagttgataa ggaataatca atctgcattg tgagcctgtt
    13501 tttaataatg caataccttg ctcccatccc tgaatcactc ttccttcgcc taaacgaaaa
    13561 ctaattggct cacctctttc tactgaagaa tcaaacatac ttccatcttc aagatatcca
    13621 ctgtaatgca cagctacagt tgcgcctgaa acagcagttt tacctgttcc ttctttaacc
    13681 ataatatact gaagtcccga gggtaattta actgtatcct tgccttgaac attgtaaggt
    13741 gttggtacaa ttttgggcga cactcgcaac aactcaactt caaaaatcaa agtggagtta
    13801 gcaggaatag ttccaacagc acgactgcca taaccagcat caggtggtat tgtcaatact
    13861 gccttgtcac cttgctgaag catactgaaa gcttcatccc atccctttat tacttgccct
    13921 gttccaacat taaacacaaa aggctctcct ctgtcaacag aactatcgaa cttagtgcca
    13981 ttctgcaatt tacctgtata atgaacggtg attctatcac ccggtgatgg cttaattccg
    14041 tttccttttt ctttaatttc aattttaact cctgattgtg ttgtcataaa atttgtttga
    14101 gcatgatgtg tttctacaga ctgcttacct gctttgcagg cagtcattat agcaagcaaa
    14161 cataacatta gttttttcat gctcggcagg atactgcatt tttttcaaaa ttgtaaaaat
    14221 catagtgtat ccggccttaa tacaattcaa agtttttcac actacaaggt gaattagttc
    14281 aagttgcgtt tggtaagtaa tgccacacat tcaatgtgat cggtctgcgg aaacatatct
    14341 acc
//
LOCUS       DAOOOL010000064        14341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_365_length_14341_cov_100.409282, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000064 DAOOOL010000000
VERSION     DAOOOL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14341
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_365_length_14341_cov_100.409282"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PKU82_06660"
     CDS             <1..1244
                     /locus_tag="PKU82_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82592.1"
                     /translation="VCMRCHLQGNAVLNNNKSFFDFKPGMKLSDVMNVFVPVYEGNGN
                     AHIMASHAERLKQSRCYIETANKNHANHQSSELRPGKNALTCITCHNPHVSVRQTNDD
                     HFNAVCKNCHHTENSLCTEKLSVREKSNDNCISCHMTFSGTSDIPHVSIHDHKISIPS
                     KDNKENEHKVFKGIYCVNNPTADKQSRGRAFIAYFERYGYDISVLDSALNCFSDAGNM
                     SLKENFNELVQIYFLKNDFAKVVALTSAMPDMLKMLNKKSYSNTHAWTAYRIGASFQA
                     IGNIEAALPYYQRAAVLAPYHLDILNKYATALSIAGNAKEAQKIYEFILKEYPKHVSA
                     LTNLGFIFLSEKNDPQQALTYYSRALALNPDYEPALMNMAGLMHFMNRDGEAVKFVQR
                     ILKINPRNEGARQLLNQLKST"
     gene            1245..2273
                     /gene="mltG"
                     /locus_tag="PKU82_06665"
     CDS             1245..2273
                     /gene="mltG"
                     /locus_tag="PKU82_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOZ82593.1"
                     /translation="MKKILFLILTLLLLAGAFVAYIFYQRVFAPNVKIKDNKSFLYIR
                     TGSNFNQVVDELTSQQILINAESFKWLAEKMNYTERVIPGRYEITDNMNNRQLLQLLR
                     SGKQVPIKLTFNNIRTKSDLASRISHQIEADSLQLIGMLNDSVLLKSESLNSENVLCL
                     FIPNTYEMYWNTDAADFFKRMKKEYDNFWTEIRKQKAAKQELTPVQVSILASIVQSET
                     KQNAEKPRVAGVYLNRLRQNWKLEADPTLVFALGDFSIKRVLNEYKTIDSPYNTYKYF
                     GLPPGPICLPDISSIDAVLNAESHQYMYFCAREDMSGFHAFAKDYNTHLLNARRYQAE
                     LNRRNIRN"
     gene            2307..3410
                     /locus_tag="PKU82_06670"
     CDS             2307..3410
                     /locus_tag="PKU82_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82594.1"
                     /translation="MRKGFLIVVAALFIAADVSADQVAALLRVKRSEQPDSLGFNLVE
                     QITKITYQLIQQNKIKLWDSPSKEIQITAASLQEIEQASSTSFKDEDVIYVYESWTEA
                     KREIQTKTIGISFSNRDSRNQEVSYGYVDYVDLARYLNEKPAEVNANGNYGETPAFYL
                     TAKKFNFSLVQFNNKVIQSADESQKTLRGFMGRSRFTVPPSDFSNEESKNIVYLVDRK
                     PGGDTLLSNNSSSFFNAVENYLLTNKEEFFNLGGDKVQNFVSGKQSLRVTSIEVNEVW
                     KKSSGRISYEPKSIRFYVNDSALNSIPLSEMTMMDLEINGQKLVLALLDKKFNFTITK
                     INSQDIALRQAFVYLKALREYHWNQLTEFVKYY"
     gene            3426..4841
                     /locus_tag="PKU82_06675"
     CDS             3426..4841
                     /locus_tag="PKU82_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOZ82595.1"
                     /translation="MATKIKFGTDGWRAIIAQDFTVENVTRVAEATALWLKAKGGNPS
                     VVVGNDCRFAGNLFAETTAKVLCANGIKVMLAPDFVSTPMVSLGTLHYKADLGIILTA
                     SHNPPSYNGFKLKGSYGGPLLPAQVSEIEDMIKDASTIDTLKLSLEDFTAKGLLQIVD
                     LETMYINKVKENFDLDAIKKSGINWAYDAMYGAGQNVMKKLFPEITLLHCDHNPGFHG
                     QAPEPIHKNLTEFSELIKKRGDIGCGLVTDGDADRIGLYDDKGNFVDSHHIILLLIHY
                     LHKYKGMSGKVCTAFSTTPKVQTLCKHYGLPIDVVKIGFKYICEIMIKEDVLLGGEES
                     GGIAIKGHIPERDGIWMGLVIWEFMAKSGKSLNELIKEVYEITGPFSFERNDLHIKEE
                     VKQKILANCLQNKYAQFGNYKVQRVDDLDGFKYFFDNTNSEWLMIRASGTEPVLRTYA
                     ESSSREKAFQILDACKQTLLG"
     gene            complement(5039..5653)
                     /locus_tag="PKU82_06680"
     CDS             complement(5039..5653)
                     /locus_tag="PKU82_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82596.1"
                     /translation="MIKVCIVEDIHEIRDGFKSVLDLSGEFKCCGTFGNAEDAIDKIP
                     ELNPEIVIMDINLPGINGIEAIKYLIKNNCNPLFLIFTVYDDDTIIFDALQAGASGYL
                     LKKTTPAQFLEAVKEMYDGGSPMSPIIARKVINSFKPGQKTNAILSVRESEILTLLAK
                     GLLYKEISEQLFISTGTVKQHIHKIYEKLHVQNRTEAINKFLNK"
     gene            complement(5699..7057)
                     /locus_tag="PKU82_06685"
     CDS             complement(5699..7057)
                     /locus_tag="PKU82_06685"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOZ82597.1"
                     /translation="MQGDKLTYKYYHLPLNVLLGVLICMMLFSIVYFTLNHKKVFIYY
                     FLYLLFTFLYFFIRHHDNSVTSYYSFQLEPFRDITWQTLSYLFYFFFAISFVDYKNIS
                     PILYKTIQVIIYLIITYLIFDIFLYQNHFFEIRNKAYNIFRITMAPIAIIIILSAFLI
                     KSPLSKILATGSLLMVSGATVTMILSLFYYPSANPFINYHMLYMQLGIILEVICFTLG
                     LSYMSKIQFKEKIIIESQLNKIKENYEIDRLQTIINTQDNERKRVASELHDDLGAGLS
                     TIRLLSELALQKTEGKTEIKRISELSNELVDSMRQIIWSMNPESSSLSDFIHYLKRYT
                     MEIIELNGKIFNFKLNQEISDYTLSPEQKRNLFLIIKETLHNSIKHSKSNEIILIYDY
                     IEKRHHLTIKDNGIGFSPDDINYKNGNGLINIQKRAMQIGCSVFVTSEITKGTITRIE
                     FS"
     gene            complement(7694..8854)
                     /locus_tag="PKU82_06690"
     CDS             complement(7694..8854)
                     /locus_tag="PKU82_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82598.1"
                     /translation="MKQIRKKHFSVKLFTSVMAFILLMNHTTSAQIDCSSASGILALM
                     AAPVAINTVDPDDFPLNPSLIVSCTTKVFKTPPASISSICTNAIKMYVFYSTTQSDLS
                     SLPNLASSQLAAVLKKPNFKATITSTSNQNEKKVSVTFPNNKFIAGQRVYYRYGKLID
                     HPTDMDPMTWSPILNFITPQHIVNLPSACINPDLKPAPENMNEIILFKIIGGSWADAA
                     GHTYQQISGEWCKSFFNTSGVIIEPITGNDPSIGPMRQKTISLPPINWGVRETNNRPI
                     NNSFTNQLTRSGENTILATFNISSMTANSTQMGPPFTNRGTRTVYLFEQQNKACAVRT
                     FDAVHGNLNITENNYIVKVDSGNTITECTDSSNDNIKTYSHGGGNTVVFPSN"
     gene            complement(8945..10174)
                     /locus_tag="PKU82_06695"
     CDS             complement(8945..10174)
                     /locus_tag="PKU82_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82599.1"
                     /translation="MRKFYSFLSILFCLNNFAHSQIIQTESPAKITPREINVASSSIF
                     DMMGVSSSQISKTSDVKDLKVDWSFKSWKLNPNIAVQGQPVWEIIYNRRDLSKYRSAS
                     YVMRTLASTDISAGTVQAENNDRRIGGAIKFNLYRKYDALRSEVYYKDIEQKYNTQFE
                     DWQQRMILLKSTIDTTTDIGKRSDLKMQISLLENEAALLNQSRKNEITSRATEFNAEN
                     WNSSFVDVAAGKIWTYSTDSAGTLKSVRINRQSGWAMWTNAGYGIGSKILITGLFRVS
                     FYDEQLNFLIKDLDTGNEITATSLVSNKIYSYGINFRYGTPSINFFSELLLEKRQLKT
                     VKDAFNKSDFVGEGNFEIIEGTLKWTALSPFTISFGGEWRISKNLLLNFGMRMEYEKN
                     WKRKTFQPIAGISCLMR"
     gene            complement(10958..11041)
                     /locus_tag="PKU82_06700"
     tRNA            complement(10958..11041)
                     /locus_tag="PKU82_06700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11005..11007),aa:Leu,seq:cag)
     gene            complement(11069..11150)
                     /locus_tag="PKU82_06705"
     tRNA            complement(11069..11150)
                     /locus_tag="PKU82_06705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11114..11116),aa:Leu,seq:gag)
     gene            11274..12380
                     /locus_tag="PKU82_06710"
     CDS             11274..12380
                     /locus_tag="PKU82_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOZ82600.1"
                     /translation="MFNKFHNSLNTLADPLWIAGPCSAEDEEHLTELAIRLKISGAHI
                     LRAGAWKPRTRPGAFEGFGAPALKWIRAAGDVSGLPVITEVASAKHVEQALAAGIDMF
                     WIGARTTVNPFLIQEIADALKGVDIPVFVKNPVNPDVKLWMGAIERFMQNGISRIASI
                     HRGFSSFENSTYRNKPNWELAIELRRLLPNVPIICDPSHISGDANMVPTLTQIAFDLQ
                     YDGVMVEVHLNPVKALSDAMQQITPEDFSRLRKQIIVRQPTAKDPVELSKLHDLRDLI
                     DELDSEIIEKIAGRMEVARKIGQYKDQNNMAILQLDRFKEILRTRTETGLRNQLTPDF
                     ILRLYSLIHEESIAQQDKQMTKPELRASSKAEKH"
     gene            complement(12389..12538)
                     /locus_tag="PKU82_06715"
     CDS             complement(12389..12538)
                     /locus_tag="PKU82_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82601.1"
                     /translation="MSATNANSAVEYSYHKAHIFFSFDYYNPVKNQSTPVFYLGSGRN
                     YYQQN"
     gene            12481..13455
                     /gene="aroB"
                     /locus_tag="PKU82_06720"
     CDS             12481..13455
                     /gene="aroB"
                     /locus_tag="PKU82_06720"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOZ82602.1"
                     /translation="MYVLCDTNTQQHCLHLLQTLFPQTKSASLYCIQQGESFKTIETV
                     TGFWKFLLQQQADKHTVIINLGGGVITDLGGFAASLFKRGVDFIHVPTTLLAMVDAAI
                     GGKNGVNIDGYKNQSGSIVLPRYVYVDTDFLESLSQDEKVNGYAEMIKHALVADQEFY
                     KVLSEQCSFEKLQSIGKAIAIKLSIVAIDPFEKKERKLLNFGHTIAHAIESLYYSQQQ
                     DISHGKAVAAGIIVESYISNKMGFISDAELKQITDFIQSLFKFDEKDIVVEKLLPFMM
                     ADKKNKQGQIRFTLLESIGKGIVDIAVPIAMIEEALVWFSKQDSGYGV"
     gene            13445..>14341
                     /locus_tag="PKU82_06725"
     CDS             13445..>14341
                     /locus_tag="PKU82_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82603.1"
                     /translation="MAYRISKTDRSVVGEIVVPVSKSEAGRVAIINALRGIKQNSDCH
                     ASDTVKLQELLFSEEQILDAADGGTTARFLMAYCVVKNKTAVITGSPRLRQRPMSDSV
                     DLLRQIGAEISYLEEEGFLPIRVRAAKLNFPEVVQTSAEKSSQHISALMMIAPLFGKD
                     FTIELNGISSSLPYIELTAYLMHQAGVNVLITENRIQINGRYNNSILSAGGDWSAASY
                     FFETVALADDADITIKNLNIQSKQGDKIIVEMIKPFGVDTYVEKDKIILRKQKKMVPD
                     YFTQSFLHFPDIVLSMATVCGGQ"
BASE COUNT         4556 a         2288 c         2749 g         4748 t
ORIGIN      
        1 atgtatgtat gcgttgtcat ctgcaaggta atgctgtatt gaataacaac aaatcatttt
       61 tcgattttaa accgggcatg aaactgagtg atgtgatgaa tgtgtttgtt cctgtttatg
      121 agggtaatgg taatgcacat atcatggcat cgcatgccga aaggcttaaa caaagcagat
      181 gttatataga aacagcgaat aaaaatcatg ctaatcatca atcatctgaa ttaagaccgg
      241 gaaagaatgc attaacctgt attacctgtc acaatccgca tgtcagtgtg cggcaaacta
      301 atgacgatca tttcaatgca gtttgcaaaa attgtcatca tacagaaaat agtttgtgca
      361 cggagaagtt aagtgttaga gaaaaatcaa atgataattg cattagctgt cacatgacat
      421 tttcaggcac atcagatatt ccgcatgtca gcatacacga tcataagata agtattccat
      481 caaaagacaa caaagagaat gaacataaag tgtttaaggg aatttattgt gtcaacaatc
      541 ccacggcaga caagcagagt aggggaagag cctttattgc ttattttgag agatatggtt
      601 atgatataag tgtactcgac tctgcactaa attgtttttc agatgcagga aatatgtctc
      661 tcaaagaaaa ctttaatgaa ttagtacaga tatattttct gaagaatgat tttgcaaaag
      721 ttgttgcact gacttcggca atgcctgata tgttgaagat gttgaataaa aaatcgtatt
      781 ctaacacaca tgcctggacg gcctatcgga taggtgcatc ttttcaggca attggaaata
      841 ttgaagcagc attgccttat tatcaacgtg ccgcagtatt agccccatac catctcgata
      901 ttttaaataa atatgctact gcactttcta ttgcaggtaa tgctaaagag gctcagaaaa
      961 tatatgagtt tattctaaaa gagtatccaa aacatgtttc agcattgaca aaccttggtt
     1021 ttattttcct ctctgaaaaa aatgacccac aacaagcatt gacatattat agcagagcat
     1081 tggcacttaa tcccgattac gaacctgcat taatgaacat ggcagggcta atgcatttta
     1141 tgaatagaga cggagaggct gttaagtttg tgcagcgtat cttgaaaatc aatcccagga
     1201 atgaaggcgc acgacaacta ttaaaccaac tgaaatcaac ataaatgaaa aaaatacttt
     1261 ttttaatttt aactttactt ttattagcag gtgcttttgt tgcatatatc ttttatcaaa
     1321 gagtatttgc tcccaatgta aagattaaag acaataaatc ttttttatat atcagaacag
     1381 gaagtaattt taatcaggtt gtagatgagt tgacatcgca acaaatttta atcaatgcag
     1441 aatcattcaa gtggctggca gaaaaaatga attatacaga acgtgtaatt cccgggcgct
     1501 atgagataac tgacaatatg aacaacaggc agttgttgca attattgcgc tctggaaagc
     1561 aggttcccat taaacttacc tttaataata tcaggaccaa gtcagacctt gcctctcgta
     1621 taagtcatca gattgaagca gacagtttac aattaattgg tatgcttaac gacagtgtac
     1681 tcttaaaatc cgaatcgtta aatagtgaaa atgttttatg tctgtttatt cccaacactt
     1741 atgaaatgta ctggaatact gatgctgctg attttttcaa acgtatgaag aaagaatacg
     1801 ataacttttg gacagagata cgaaaacaaa aagcggcaaa gcaagaattg acccctgtgc
     1861 aggtgagcat attggcatct attgttcagt cagagacaaa gcaaaatgca gaaaaacctc
     1921 gtgtggcagg agtttatctt aacaggctca gacaaaactg gaaattggag gcagacccaa
     1981 cacttgtttt tgctttaggt gatttttcga ttaaacgtgt gctgaatgaa tataagacta
     2041 ttgattcgcc ttataatact tataaatatt ttggactgcc tccgggtcca atttgtttac
     2101 ctgatatttc aagcattgat gctgttctca atgctgaaag tcatcagtat atgtatttct
     2161 gcgctcgtga agatatgagt ggttttcatg cttttgcaaa ggattataat acgcacctgc
     2221 tcaatgccag acgctatcag gctgaattaa acagacgtaa cattcggaat taattcactg
     2281 taaaacggta tttttacgcc aataaaatga gaaaagggtt tttaattgtt gttgcagcac
     2341 tttttattgc tgctgatgtt tctgccgatc aggtggctgc actacttcgt gtaaagcgta
     2401 gtgagcaacc cgattcactt gggtttaatt tagttgaaca gattaccaaa ataacctatc
     2461 aacttataca gcagaacaaa attaaattgt gggactcacc atctaaagaa attcaaatta
     2521 cagctgcatc gttacaggaa attgaacaag catcatcaac ttcatttaaa gatgaggatg
     2581 ttatttatgt atatgaaagt tggactgaag ctaaacgtga aattcaaact aaaacaatag
     2641 gaataagttt ttcaaaccgc gacagccgca atcaggaagt atcctacggt tatgttgact
     2701 atgtcgattt agctcgctat ttgaatgaga aaccagcgga agtaaatgct aatggtaact
     2761 atggcgaaac acctgcattt tatctcacag caaaaaaatt taatttcagt ttggtgcagt
     2821 ttaataataa agtaattcag agtgcagatg agtctcagaa gacattaaga ggttttatgg
     2881 gaagatcccg atttactgtt ccaccatcag attttagtaa tgaagagtct aagaacattg
     2941 tttatttagt tgacagaaag cccggaggag atactttgtt atcgaacaat agcagtagtt
     3001 tttttaatgc agtagagaat tacttattga ccaacaaaga agagtttttt aatttgggtg
     3061 gtgataaggt gcaaaatttt gttagcggca aacaaagcct tagggttact tcaattgaag
     3121 tgaatgaggt atggaagaaa agtagtggac gaatttctta tgaacctaag agtatccgtt
     3181 tttatgttaa tgattctgca ttaaatagta ttccactttc tgaaatgact atgatggatt
     3241 tagaaatcaa tggtcagaaa ttagtccttg ccttgcttga taaaaaattt aatttcacaa
     3301 taacaaaaat taattcacag gatattgcac taagacaggc ttttgtttat ctaaaagcac
     3361 ttcgtgagta tcactggaat caactaacag aatttgtaaa atattattga acattataaa
     3421 aatttatggc aacaaaaatt aaattcggta ccgatggatg gcgtgcaatc attgcacaag
     3481 attttacagt agaaaatgtt acacgtgtag cagaagctac agcactgtgg ctaaaggcaa
     3541 agggtggtaa cccttcagta gttgtaggca acgattgtcg ttttgcagga aatctctttg
     3601 ctgagactac tgcaaaagtt ttatgtgcaa acggaattaa agtgatgttg gcaccggatt
     3661 ttgtttcaac gcccatggtt tcgctaggta cattacatta caaagccgac ttgggaatta
     3721 ttctaacagc aagtcacaat ccaccatcgt acaatggatt taaattgaaa ggttcttatg
     3781 gcggaccgtt attacctgca caggtttcgg aaattgaaga tatgataaaa gatgcttcaa
     3841 ccatagacac tttaaaattg tctttggaag attttactgc gaaaggattg ctacagatag
     3901 ttgaccttga aacaatgtat atcaacaagg ttaaagaaaa ctttgacctt gacgctataa
     3961 aaaagagtgg aattaactgg gcttatgatg ccatgtatgg tgctgggcag aatgtgatga
     4021 aaaaattatt tcctgaaatt accttgttgc attgcgacca taatcccgga tttcatggtc
     4081 aggcaccgga gccaattcat aaaaacctga cagagttttc ggagttgata aagaagagag
     4141 gtgatattgg ttgcggatta gttactgatg gcgatgccga ccgtatagga ctttatgatg
     4201 ataaaggaaa ttttgttgac tcacatcaca tcattctgct gcttattcat tacctgcata
     4261 agtacaaagg aatgagtgga aaagtttgta cggcattttc taccacacct aaagtgcaga
     4321 cgctatgtaa gcattacggt ctgcccattg acgtagttaa gattggtttc aaatacatct
     4381 gcgaaataat gattaaagaa gatgtattgt taggtggaga agagtccggt ggtattgcca
     4441 ttaaaggaca tattccggaa cgtgatggta tttggatggg attggtaatt tgggagttta
     4501 tggctaagtc aggaaaatca ctgaatgaac ttattaaaga agtatatgaa ataaccggtc
     4561 cattttcttt cgaacgaaac gatcttcata tcaaagaaga agttaaacaa aaaattcttg
     4621 caaactgcct tcagaataaa tacgcacagt ttggcaatta caaagtacaa cgtgttgatg
     4681 accttgacgg atttaaatat ttcttcgaca ataccaacag cgaatggctg atgatccgtg
     4741 ccagcggaac agagcctgtt ctcagaacct atgcagaatc atcttccagg gaaaaagcat
     4801 ttcagatttt agatgcttgt aagcagacac tgttggggta gtgccatata ttacttgcgt
     4861 gaagtgctca aagttatctt gtaagatttt tttactaaac gtgcgtaaag ttgtttactt
     4921 aacggctgcc gtcttacata aattacagca gactttgggg ggtatttaat tttttataat
     4981 ttaatagaaa attatacgca agttctaatc ggctcaggtt ttgaaatacg ttctactttc
     5041 atttgttcaa aaacttatta attgcttcag ttctattctg aacatgaagc ttttcataaa
     5101 ttttatgaat atgctgtttt actgttccgg tactaatgaa aagttgttct gatatttctt
     5161 tatataacag tccctttgca agtaatgtta gaatttcaga ttcacgtaca cttaaaatgg
     5221 catttgtttt ttgcccaggt ttaaatgaat ttatcacttt tcgtgctata attggactca
     5281 tcggtgaacc tccatcgtac atctctttaa ctgcttccag aaattgtgca ggtgttgttt
     5341 tttttagtag atacccactt gcacctgctt gcaacgcatc aaatattatt gtatcatcat
     5401 cataaacggt aaaaattaga aacaatgggt tacagttgtt ttttattaaa tacttaattg
     5461 cttctattcc atttatacca ggtagattga tgtccattat cacaatttct ggatttaact
     5521 caggaatttt atcaatagca tcttcagcat tgccaaatgt gccacaacat ttgaactcac
     5581 ctgaaagatc caagacactc ttaaatccgt ctcttatttc atgtatgtcc tcaacaatgc
     5641 ataccttaat catgttgcaa tattaattat ttgaggggta aattagatat aacttttgtt
     5701 aactaaattc gattcttgtg atagttcctt tagtaatttc tgaagttaca aatacagaac
     5761 agcctatttg cattgctctt ttttgaatgt taattaaacc attcccgttt ttgtaattaa
     5821 tatcatcggg agaaaatccg ataccattgt ctttgatagt taggtgatgc cttttttcaa
     5881 tatagtcgta tataagaatg atttcgttag atttagaatg cttaatgctg ttgtgcaaag
     5941 tttcttttat tatgagaaat aaatttcttt tttgctcagg actaagtgta taatcagaaa
     6001 tctcttgatt taatttaaaa ttaaatattt ttccgtttaa ttctataatt tccatcgtat
     6061 atcgctttaa ataatgaata aagtctgata aactgctgct ttcaggattc atgctccaga
     6121 ttatttgtcg cattgagtca acaagttcat ttgataactc actgattcgt tttatttcag
     6181 tttttccctc ggtcttttga agagctagtt cgctcagtaa tcgtatggtt gaaagacctg
     6241 cacctaaatc atcatgtaat tctgaggcta ctctttttct ttcattatcc tgtgtgttga
     6301 ttatagtttg cagtctatca atttcatagt tttcttttat tttattcaat tggctctcta
     6361 ttattatttt ttccttaaat tgtatctttg acatatagct taatcctagt gtaaaacaaa
     6421 ttacttccaa tattatacct aattgcatat ataacatatg gtaattaata aatggatttg
     6481 ctgatggata ataaaataat gatagaatca tggttaccgt agcaccgctt accataagta
     6541 aactccctgt cgctaaaatt tttgacagag gactttttat taaaaaagca ctaagaatta
     6601 tgattattgc aattggggcc attgtgattc tgaaaatgtt ataggcttta tttcgaattt
     6661 cgaagaaatg attttgatat aaaaaaatat caaaaatcaa atacgtgatg attaggtaga
     6721 ttattacctg tatggttttg taaagtattg gagaaatgtt tttataatct acgaaactta
     6781 ttgcgaaaaa aaaataaaac agataagata aagtttgcca ggtaatatct cgaaagggtt
     6841 ctaattgaaa agagtagtag gaggtaacag aattgtcgtg atgacgtata aaaaagtata
     6901 aaaaagtaaa aagtaaataa aggaaatagt atatgaaaac ttttttgtga ttaagtgtaa
     6961 aatagacaat ggaaaacaac atcatacaaa ttaagacgcc taataaaaca tttaatggta
     7021 aatgataata tttgtaagtg agtttatcgc cttgcattaa tttaaaaaga acatcttcgg
     7081 ttttggagga gctttctgtg tttataaata acaaaagttt gttaaatggc ttgtagtgtc
     7141 cagatatatt tatccatagc ttatatgaag aattaggcat aaaggttaat ggaaagataa
     7201 aggaattgct ttttgttact acgtttttca gataataata ttctagatcc cctaacgatt
     7261 cattaaatag gtagattgat gatgaaggga aggctcctat ataaaagtat ctggtagttt
     7321 tatgtgctga attattcttc cattctgaat atatccagat gccttttata gatttatcct
     7381 taatagtata aggtatatta atctttacat cggattgtaa ttttggaaag aaattctgat
     7441 cagcaacttg attgaagtgt agtgttaaac ttgaatcata gaataagctg caacgaaaat
     7501 agggttcttt caagtcatca gatatcaggt ttgactcacc tacattagac ttagtatatc
     7561 cgttacggct taatatcaga caagtaaata tgaagaaaca aatatgagtg attcgcattt
     7621 ttgtaatgcg aatgtaattg aaaaaataac aggtaatgat ttctcattac ctgtttcatt
     7681 gagttaattt taattaatta cttggaaaca cgactgtgtt gccccctcca tgactgtaag
     7741 ttttaatatt gtcatttgaa ctgtctgtac actctgttat tgtattgcct gagtccactt
     7801 ttacaatata attattttct gttatgttca gatttccatg aacagcgtca aatgtacgta
     7861 cagcgcaggc tttattctgc tgttcaaata agtaaaccgt tcttgttcct ctgttggtaa
     7921 agggaggccc catttgggtg gaatttgcgg tcatggaaga tatattaaat gttgccagta
     7981 ttgtgttttc acctgatcgt gtcaactgat ttgtaaagga attatttata gggcggttat
     8041 ttgtttctct cacaccccag tttataggtg ggagagatat tgttttttga cgcataggtc
     8101 caatgcttgg gtcgtttcct gttatcggtt ctatgataac cccagatgtg ttaaaaaaag
     8161 atttacacca ttctcctgaa atttgttggt atgtgtgacc ggctgcgtca gcccaacttc
     8221 ctccgataat tttaaataat attatttcat tcatattttc cggtgctggc tttaagtcag
     8281 gattgataca agcggatggt aaatttacta tatgttgagg tgtaataaaa tttagaatag
     8341 gactccatgt cattgggtcc atgtctgtag ggtggtcaat taatttgcca tatctgtaat
     8401 atactctttg tcctgcaata aacttgttgt taggaaaggt gacacttact tttttttcat
     8461 tttgattaga tgtcgatgtt atagttgctt taaagttggg tttttttaaa actgcggcaa
     8521 gctggcttga tgctaaattt ggcaaactag aaagatcgga ctgtgtagta gagtaaaaaa
     8581 catacatctt aatggcatta gtacatatgg atgaaatact agcaggtgga gttttgaata
     8641 ctttagtagt acagctaaca attaaggacg gatttaatgg gaaatcatca gggtcaacag
     8701 tgttaattgc aacaggtgct gccattaatg ctaaaattcc agatgcagaa ctacagtcta
     8761 tttgggcgga tgtagtatga ttcattagca gtataaaagc cattacagag gtaaacaact
     8821 ttactgagaa atgttttttt cttatttgtt tcatcttaat atgttttaat ttgattgtta
     8881 tgaactattg caaatgttca gccctttttt aaatagtgct atagaactta agttagtttt
     8941 tatatcatcg catcagacat gatattcctg caattggttg aaatgttttt cttttccagt
     9001 ttttctcata ttccatacgc atcccgaagt ttagtagtaa atttttactg attcgccatt
     9061 caccgccaaa agaaattgta aatggtgaaa gggctgtcca ttttaatgtg ccttcaatga
     9121 tttcaaagtt gccttcaccg acaaaatcac ttttattgaa ggcgtctttt acggttttta
     9181 attgccgttt ctccaataaa agttctgaga aaaaattgat agatggtgta ccatacctaa
     9241 aatttattcc ataggagtaa attttattgc ttactaatga agttgctgta atttcattcc
     9301 ctgtgtctaa atccttgatt aagaaattta attgttcatc ataaaatgat actctgaata
     9361 ggcctgtaat aagtattttg ctccctattc catatccggc attagtccac attgcccatc
     9421 ctgattgtcg gtttattcgt acagatttta atgtgccagc actatcagtt gaataagtcc
     9481 atattttccc agccgcaaca tctacaaacg aactattcca attttcagca ttaaattctg
     9541 ttgctcgcga agtaatttcg ttttttctgg attgatttaa taatgcagct tcgttttcca
     9601 gaagtgaaat ttgcattttt aggtcagatc gtttgccaat atctgtggtt gtatcaattg
     9661 tactttttaa tagtatcatc ctttgctgcc aatcttcaaa ctgagtatta tatttttgct
     9721 caatgtcttt ataataaacc tcacttctta aggcatcata tttccggtat agattaaact
     9781 ttatagctcc tccaattcgt ctatcattat tttctgcctg tacggttcct gcagaaatat
     9841 ctgttgatgc tagggttcgc atcacataac tagccgaacg atatttactt aaatctctgc
     9901 ggttataaat aatttcccat actggctgac cttgtactgc aatgttcgga tttaacttcc
     9961 aggatttgaa tgaccaatct acttttaaat cttttacgtc tgaagttttt gaaatttgtg
    10021 aggagcttac tcccatcatg tcgaaaatgg atgatgatgc cacgttgatt tcacggggtg
    10081 tgattttagc aggtgactct gtttggatta tttgtgaatg agcaaagttg tttaaacaaa
    10141 atagaattga taagaaagaa tagaatttac gcataactaa tcagattaaa ttattgacag
    10201 taaatgagta aaattcgcac agtaagttct aatgtacaat ataacttagg ttagtttgtt
    10261 ttcgggtaat ctctactcac ataccaaaaa tagttcatcg tgtgacatat agtttttcaa
    10321 ctacactttg aggaatactt ttttacgtta agttgttgaa cgaattatct cttggtagtc
    10381 ttagtgtgaa aatagcagag tttaactatc ttaaagtagc tttgatttat agtaataaga
    10441 caaatttttc tgtaccaatg tttttcaact tcttgccgat ttgagatttt gattcttgat
    10501 aatttttgca gtaaaactct gacataaata ttgttctttt attggtactc atcagctgaa
    10561 tgtaagcata gaattgttat tcagcaggtg gaggatctgt cttgcatccc catgtatttc
    10621 tgtgattaga aatgttttgt catgaccaca tcaccaaagt ttcttgtctt gattttatct
    10681 tgttctatag aaattttgat agcctttgtt cttagttcac cctagacaaa ccagatttga
    10741 aaaatagcac tttaggcata ttagttgaga taattattat tgtgtttata gattaataat
    10801 gtaattctgt catgaattgt cattcgaaat tacaaaatca gaaaacgttt atttatatct
    10861 tattcaaatt tatttttgta gggtttattg tcctttttag taattcaggt ttagtaaaaa
    10921 taagctagtg cataaatagg gtggctctat aaaaatgtgc ccgagaggag actcgaactc
    10981 ctacaggttt taccccacag gcttctgaga cctgcgtgtc taccaatttc accacccggg
    11041 caaatatttt ttcagcaatt agtttaagtg cccgggacga gattcgaact cgtacaccta
    11101 ttggcgccac cccctcaaga tggtgcgtct accaatttcg ccacccgggc attatgggct
    11161 gcaaaagtaa taaatttaac ttctgtaatt ttaatcccgc tgaatattcc aatacactat
    11221 tgcatagcaa tgctgctttc tgcacttgaa atgtgttctt tatctttgca ctaatgttta
    11281 ataagtttca taactcactg aacactcttg ccgacccatt gtggattgca ggtccatgca
    11341 gtgccgaaga tgaggagcat ctgactgaat tagctatcag gctaaagatt agcggtgcac
    11401 atattttgcg tgccggtgct tggaaacctc gcacccgacc cggtgccttt gaaggctttg
    11461 gcgctcctgc attgaaatgg atcagagcag caggagatgt ttccggccta ccggttatta
    11521 ccgaagtggc aagcgccaaa catgtggagc aggcacttgc tgccggcatt gatatgtttt
    11581 ggattggcgc acgtacaact gtaaatccat ttttaattca ggaaattgct gatgctttaa
    11641 aaggtgtaga tattcctgtg tttgtaaaga atcccgtcaa ccctgatgta aaattgtgga
    11701 tgggtgccat cgaacgtttt atgcaaaatg gaattagtag aattgcttct attcatcgtg
    11761 gtttttctag tttcgaaaac agtacatata gaaataagcc caattgggaa ttagccattg
    11821 aactcagacg cttgttgccc aacgttccca ttatttgcga cccaagtcat attagcggtg
    11881 atgctaatat ggtgccaaca ctgacacaaa ttgcattcga tttacagtat gatggtgtga
    11941 tggtagaagt gcatttaaat cctgtaaagg cattgagtga tgcaatgcag caaatcacac
    12001 ctgaagattt tagtcgttta agaaagcaga ttattgtaag acaacctacg gcaaaggatc
    12061 ctgttgaact aagtaagctg cacgatttac gcgatctgat tgatgaattg gatagtgaaa
    12121 tcattgaaaa gatagcgggt agaatggaag tggcacgaaa aatagggcag tataaggatc
    12181 agaataatat ggctattctg cagttagatc gttttaagga aattctcaga acaagaactg
    12241 aaacagggtt acgaaaccaa ctcacaccag attttatttt aagattatat agcctgatac
    12301 atgaagagtc aattgcgcaa caggataagc agatgacaaa accggaactc agagcctcat
    12361 caaaagcaga aaaacattag aggggtgatt aattttgctg atagtagtta cgtcctgacc
    12421 caaggtagaa aacaggagtt gattgatttt ttacaggatt gtaataatca aaagagaaaa
    12481 atatatgtgc tttgtgatac gaatactcaa cagcactgtt tgcatttgtt gcagacattg
    12541 tttccgcaaa caaaatctgc ttctttatat tgcatacaac aaggagaatc ttttaaaaca
    12601 attgaaacgg taacgggatt ctggaagttt cttttacaac aacaggctga taagcatact
    12661 gtgattataa atctgggtgg aggtgtaatt acggatttag gaggctttgc ggcttctctg
    12721 tttaaaagag gagttgattt tattcatgtt cctacaacct tgctggcaat ggttgatgct
    12781 gctattggag gtaagaatgg tgtaaatatt gatggctata aaaatcaaag tgggagtatt
    12841 gttttgccaa gatatgttta tgtagataca gattttttag agtcgctttc acaagatgaa
    12901 aaagtcaatg gctatgcaga aatgataaaa catgcacttg ttgccgatca agaattttat
    12961 aaagtacttt cagagcaatg cagttttgaa aagttacaaa gcattggaaa ggctattgca
    13021 ataaaactaa gtattgttgc aatagatcca tttgaaaaga aggaacgtaa gttgctcaac
    13081 tttggccaca ctatagctca tgcaatagag tcgttatatt attcgcaaca acaagatatt
    13141 tcacatggta aggcagttgc tgccgggata attgttgaat cttacatctc taacaaaatg
    13201 ggctttattt cagatgctga gctaaagcaa atcactgact ttattcaatc actgtttaag
    13261 tttgatgaaa aagatattgt tgtagaaaaa ttgttgccat ttatgatggc tgataagaaa
    13321 aacaaacaag ggcaaatacg tttcacattg cttgagtcta ttggtaaagg tattgttgat
    13381 atcgctgttc cgattgcaat gattgaagag gcattggttt ggttttctaa acaggattca
    13441 ggttatggcg tatagaattt caaaaactga cagaagtgtt gtaggagaaa ttgttgttcc
    13501 tgtttcaaag agtgaagcag ggcgagttgc tatcattaat gctcttagag gaattaaaca
    13561 gaatagtgat tgtcatgcat cagatacagt taagttgcaa gaactgcttt tttcagaaga
    13621 acagatatta gatgcagcag atggcggaac tacagcccgg tttcttatgg cttactgtgt
    13681 tgttaagaat aaaaccgctg taattacagg aagtcctcga cttcggcaac gcccaatgag
    13741 tgattctgtt gatttgctca ggcagatagg agcagagatt tcttatttag aagaagaagg
    13801 ttttcttccg atcagagttc gggcggcaaa actgaacttt cctgaagtag ttcagacatc
    13861 agcagagaag tcttctcagc acatcagtgc cttgatgatg attgctcctt tgtttggtaa
    13921 agattttact attgaactga atggcatttc aagttcattg ccatatattg aacttactgc
    13981 gtatttgatg catcaggcag gcgtaaatgt gctgataaca gagaatcgaa ttcagataaa
    14041 tggaagatat aacaatagca tactttctgc cggtggtgac tggagcgctg cctcatattt
    14101 ttttgaaact gttgcattgg ctgatgatgc agacattaca attaaaaatc tgaatattca
    14161 gagtaagcaa ggtgataaaa ttattgtcga aatgataaag ccatttggcg tagatactta
    14221 tgttgagaag gataaaatta ttctaaggaa acaaaagaaa atggtgcctg attattttac
    14281 acagagtttt ttacattttc ccgatattgt attaagtatg gcaactgttt gtggtggaca
    14341 a
//
LOCUS       DAOOOL010000065        14072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_375_length_14072_cov_58.093886, whole genome shotgun sequence.
ACCESSION   DAOOOL010000065 DAOOOL010000000
VERSION     DAOOOL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14072
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_14072_cov_58.093886"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKU82_06730"
     CDS             complement(<1..266)
                     /locus_tag="PKU82_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOZ82604.1"
                     /translation="MSSEKKEKIKFAVIGCGHIGKRHAEMVRRNNECELVAMCDIRQE
                     NEVGIADFNVPFFQSVDAMLTSIPDIDVVCVCSPNGVHAEHSLKA"
     gene            complement(282..854)
                     /locus_tag="PKU82_06735"
     CDS             complement(282..854)
                     /locus_tag="PKU82_06735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOZ82605.1"
                     /translation="MMDYFKHESSYVDDGCTIGKGTKIWHFSHIMPGCRIGEGCNIGQ
                     NVVISPDVVLGNNVKVQNNVSLYTGVVCEDDVFLGPSMVFTNVTNPRSAVNRKNEYKK
                     TVVKKGASIGANATIVCGHDIGRFAFIGAGAVVTKDVADYALMAGNPARQMGWMSEYG
                     HRLKFDKDGMAVCPESGEKYLLKNNQVTKI"
     gene            complement(979..1920)
                     /locus_tag="PKU82_06740"
     CDS             complement(979..1920)
                     /locus_tag="PKU82_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ82606.1"
                     /translation="MKKKPPFHNLRSIFKQYNTFTYSERKGIIVLSIIIVVLLLTLYG
                     LKFYEPPIAINTSRLDSAVAQWNLLQADSIVVKDATKLSENNSPQTTRILFPFNPNNL
                     ADSLWTKLGLSEKQIASIKKYEERGGKFRIRSDVKKLFVITPSFYDSIYSFILLPDSM
                     PTFAQNKYQQDSIKVKKEWTIEPIEINLADEEEWQKLPAIGPGRAAAIVSYRNKLGGF
                     TSINQLHEVNAIVDSVFEKISPYLLCNIKPIQKKNINTDDAFSLRHPYISSSLATIIV
                     NYRKMHGDYKSTEQLRDLKIIKPEDFEKLAPYIVVGF"
     gene            2061..2417
                     /locus_tag="PKU82_06745"
     CDS             2061..2417
                     /locus_tag="PKU82_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOZ82607.1"
                     /translation="MSVLPPCPLCHSIYTYENGNLMVCPECFYEWNPEEQQSVSEETT
                     SADSGKVFDANGNELQNGDAVIVIKDLPVKGAPKPVKAGTKVKNIRLVEGNHNIDCKI
                     DGFGSMALKSEFVRKA"
     gene            complement(2425..3336)
                     /locus_tag="PKU82_06750"
     CDS             complement(2425..3336)
                     /locus_tag="PKU82_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOZ82608.1"
                     /translation="MIFPLPLKAGDTIAIVATARKVSRDEMAPAIDILQQWGLHVTEG
                     KNLYADKNQFAGDDTLRAEDFQWALDNPNIKAVLFARGGYGSVRIIDSINFSEFKKNP
                     KWLIGFSDITTIHSHVHKQLNIVTLHAPMAINFPKSPNVILKQIKDFLFGNSIVLNAA
                     THPLNRTGNATGQLVGGNLSILYSLAASASDIDTNGKILFLEDLDEYLYHIDRMMMQL
                     KRSGKLHGLAGLVVGAMSDMKDNTIPFGKTAEEIIAEHVSEYNFPVCFNFPAGHITEN
                     NPLAMGSYAELIVENKVSLKMNHKILS"
     gene            3474..5567
                     /gene="metG"
                     /locus_tag="PKU82_06755"
     CDS             3474..5567
                     /gene="metG"
                     /locus_tag="PKU82_06755"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOZ82609.1"
                     /translation="MSRYLITAALPYANGPVHIGHLAGCYIPADTYSRYLRLKGEDVK
                     FICGSDEHGVPITIKARQLGITPQQVVDKYHQMMKSSFEKLGISFDHYSRTSSPIHHQ
                     TASDFFKTLYDKGVFIEETTEQFYDEEAKQFLADRYITGTCPRCNNPNAYGDQCENCG
                     ASLNPTDLINPKSKLSGNTPVLRPTKNWFLPMGEIQKTKEFQEYIKRFDNWKPNIKGQ
                     CSSWLNEGLHPRAMTRDLDWGVKVPLPNTEGKVLYVWFDAPIGYISATKEFLPNNWKD
                     YWQKQNKEEENPKLIHFIGKDNIVFHAIIFPMMLMQHGGFVLPDNVPANEFLNLEGDK
                     ISTSRNWAVWLHEYLEDFPGREDELRYVLTSIAPETKDSEFTWKDFQARVNNELGAIV
                     GNFVNRVVVLSNKYFKGNLTTDREIAASPFENELKKLLQSYTKAMENFQFRDAQSIAM
                     DFARIGNKYLTECEPWKKYKADDVTSVNQTREVLIHCLSLVSKMAVLFQPFLPNASAK
                     MLDMLKVDSEKRKLTKNGFEDNKISLNNIILNIDSLILFQKIEDADIEKQIQKLHDKK
                     VMNTEQNQQAEVVSETTIAPQLAETTFDDFSKMDIRVATILEAERVPKTDKLLKLLLD
                     TGIDKRTVVSGIAAWYDPEKIIGQKVVMLANLAPRKIKGIESKGMILMAQNDKGELSF
                     ITPSKPQMDNGNTVK"
     gene            5594..6988
                     /gene="fumC"
                     /locus_tag="PKU82_06760"
     CDS             5594..6988
                     /gene="fumC"
                     /locus_tag="PKU82_06760"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOZ82610.1"
                     /translation="MEFRKEKDTMGIVEVPANVYWGAQTQRSIENFKIARDTNKMPKE
                     IIEAFAYLKKAAAQTNFEAGVLPKEKNELIARVCEEILEGKLNDQFPLVVWQTGSGTQ
                     SNMNVNEVVAYRAHVINGGSLLDEKKAIHPNDDVNKSQSSNDTFPTAMHIAAYKILID
                     VTLPGIKKLRDTLKAKSKEFMNVVKIGRTHLMDATPLTLGQELSGYVSQLDHGIRAIE
                     HTLPHLAELALGGTAVGTGINTPKGYDVNVAKHIASLTRLPFVTAENKFEALAAHDAI
                     VEAHGALKTVACSLMKIANDIRLLASGPRCGIGEVYIPDNEPGSSIMPGKVNPTQCEA
                     MTMVAAQVLGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGEVCISFNDKCAIG
                     IAPLHDNIKKNLENSLMLVTALNTHIGYYKAAEIAQTAHKQGKTLKQTAIDLGYVTSE
                     QFDAWVKPEDMVGL"
     gene            7021..7434
                     /locus_tag="PKU82_06765"
     CDS             7021..7434
                     /locus_tag="PKU82_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOZ82611.1"
                     /translation="MKKLIIFAFIFLTSVVAFAQSGDVFTTVEKMPEFPGGQTGLQEY
                     LLKNIKYPEEARTNVVEGVSYINFVVSSTGDVTGARVLKGNNEQFNQEALRVVSAMPR
                     WQPGMQGGQTVSVQYTLPVRFSLTVPDKEGKKHKK"
     gene            7525..8361
                     /locus_tag="PKU82_06770"
     CDS             7525..8361
                     /locus_tag="PKU82_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOZ82612.1"
                     /translation="MNRFKNILINGKHGKPIVTDMFFNGNKEPMPVIIFAHGFKGFKD
                     WGPYDMIAESFAHSGFVFIKFNFSFNGTTPDNLTEFADLEAFGQNNFTKELDDLDTLL
                     NWLWQHPHKERFDLNTIYLAGHSRGGAISIIKAAEDGRISKIASWASPIEFSKYISPQ
                     QLSLWQQAGVIYIENARTHQQMPLYYQLAEDAINNAQRINIHKAVKQLSIPHLIIHGT
                     EDETVSFSDALVMHSWNTRACLTPITSANHSFGAEHPYAGQLLPPHYQQVVNETIAFF
                     RG"
     gene            8446..10542
                     /locus_tag="PKU82_06775"
     CDS             8446..10542
                     /locus_tag="PKU82_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOZ82613.1"
                     /translation="MAIIGKIRSKAGLLIGIIGLSLVAFILGDLLTSNRSFIQGNTTT
                     AGIIGGKKISIQDFEAKVQQNIENYKLSQGKDQIDQQTTDQLRDQTWGQMLNEELIGK
                     QIAKTGLVVSSDELFDMVQGNDIHPQIKQAFSDPNTKQFDKKNVIQFLKNMDNDQTGR
                     TRAQWVNFENSIKEERLNQKYFDLIKKGLYVTTAEAKLDFEAKNKTESARYIMLNYST
                     IADSTVKLTDADLQKAYNENIKSYEQPEESRKLDYVTFDVVPSNEDRAVAEKYITDLV
                     PAFTASTDDSLFVAQNSDFPVDNSYHKTGTLSPAIEAQMFAEAVGFVSAPYMENNAYY
                     VAKLEGSKMLPDSVQARHILFKTEGKDSLHVKATADSIYNLIKGGAKFDQFAMLSEDP
                     GSAIKGGDLGWFKPGMMVPEFNDFCFDNKKGSLGVVKTNFGYHIIEITDQAGMSKNVR
                     VYAVGRKIEASSKTYQGVFAKANDFAAKNNNGDSYTKAVKDEGLNARNIESLKENDKT
                     VPGLENARELIRWAFKAKKNDVSTPFELGDKFVVAHLMQIKPEGTLALEDVKEQVEVE
                     ARKNKKAEMLIEKINATGSAQSLDGLASKLNIAVQPLENVTFNSPYIPNLGAEMNVIG
                     TMFGIGKDKVSKPIKGEQGVFVVQVTDVKEPQAPANFNESKTQAEQQLQNRSQYETFN
                     ALKEKAKVKDYRGKFY"
     gene            complement(10759..11451)
                     /gene="purQ"
                     /locus_tag="PKU82_06780"
     CDS             complement(10759..11451)
                     /gene="purQ"
                     /locus_tag="PKU82_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOZ82614.1"
                     /translation="MKFGVVVFPGSNCDEDMVYVLRNILKQPVEKLWHKEHSLRGCDF
                     IVLPGGFSYGDYLRSGAIARFSPIMQEVMEFAAKGGYVLGVCNGFQILCESQLLPGAL
                     LHNNSHKFICKNVFIKAETDETILTSSLKIGSAIKIPIAHGEGRFFADEATVNQIESN
                     NQVLFRYCDENGYVTEAANPNGAINNIAGICNEKRNVFGMMPHPERAADTLLGNTDGL
                     GIFKSILNYVDA"
     gene            complement(11512..12246)
                     /locus_tag="PKU82_06785"
     CDS             complement(11512..12246)
                     /locus_tag="PKU82_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOZ82615.1"
                     /translation="MFSFFKRKDSTPANFLEVKTDMHSHLIPGIDDGAKTIEDSLTLI
                     KELHALGYTKLITTPHIMSDFYRNTPETILAGLEEVRKAVKAENIPVTIEAAAEYYLD
                     DGFIHKLEEEKLLTIGNNNHLLFEISYVNAPDNLLQVIFRMQVLGYKPIMAHPERYPF
                     WGNNFDFYGSLREQGVLLQINVNSLGGYYGPDAKRIAEKLIEKEWVDLIGTDTHATKH
                     IQALHKTVREKSFRKLLEFNLLNKHL"
     gene            complement(12350..13615)
                     /locus_tag="PKU82_06790"
     CDS             complement(12350..13615)
                     /locus_tag="PKU82_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ82616.1"
                     /translation="MAVSSLNKIATSLAQADLLKNLSADLIHLLSTISTSVHFNKGQT
                     VFKSGDKANKIFFIEKGTISIVENEIELLKLQTNDCFGEEFVLPDGVYQSSAVAADDV
                     VLLQINRQDFFAVLSKHHESFNTILIVFIQKMYSQNQLKHTVFVQRLDRLNKELSKAK
                     NDVEVRTIEMIKQEKLASLGLLSAGIAHELQNPLNFVNNFAELSGEIIGEIKSTNNTT
                     EKDTLLNDVAGNIEKIQQHGMRASRIIKRIVSFSRGNTGDFELTNVNTICKEYVYLAT
                     AGIKSNIFGFECNIVESYNEQLPMINTNAQDLGRVILNIVNNAFYALNERNKNSPANE
                     NYHPELQIITKNVNKKIYLHIKDNAMGIPAELQAKIFEPFVTTKPAGEGTGLGLSICR
                     DIIRNLKGDIMLISENGKGTEFIITLPVE"
BASE COUNT         4563 a         2572 c         2696 g         4241 t
ORIGIN      
        1 gcttttagtg aatgttcggc atgaacacca ttgggtgagc aaacacacac tacatcaata
       61 tcaggaatgc tggtgagcat ggcatcaaca gactgaaaaa atggaacatt aaagtcggca
      121 atacctactt cgttttcctg tctgatgtcg cacatggcaa caagctcaca ttcgttgttg
      181 cgtctaacca tttcggcatg acgtttacca atgtgcccac aaccaattac agcaaatttt
      241 attttttctt ttttttctga gctcataaaa gtgcggtctt tctaaatttt tgttacctga
      301 ttatttttca gcaggtattt ttctccgctt tccgggcaaa ctgccattcc atctttatca
      361 aacttcaggc gatgaccata ttcactcatc caccccattt gacgggcagg attaccagcc
      421 atcaacgcat agtcagcaac atcttttgtt acaactgcac cggcaccaat aaaggcaaaa
      481 cggccaatat cgtgcccaca aacaatagtg gcattagcgc ctatggaagc accttttttc
      541 actacagttt ttttatactc atttttgcgg tttacagcac tgcgcggatt ggttacattg
      601 gtaaaaacca tacttggacc cagaaataca tcgtcctcac aaactactcc cgtataaagt
      661 gaaacattgt tctgaacctt aacattgttg cccagaacaa catcaggaga gataaccaca
      721 ttctgaccga tgttacaacc ttcgccaatg cgacaaccgg gcataatatg tgaaaagtgc
      781 catatcttgg taccctttcc tatggtacat ccatcgtcaa cataggacga ctcatgttta
      841 aaataatcca taatcgcttc cttaacccgc tttaataggg gtgcaaattt actttaattt
      901 tgttaagaat gatagtctgt ttttagatta agatggtcat tatcttacat ttttgagttt
      961 ggccgtagtt ttttgctgct aaaaacccac aactatatag ggtgcaagtt tttcaaaatc
     1021 ttccggttta attattttta aatcacgtaa ttgttctgtt gatttatagt caccatgcat
     1081 ttttctgtag tttacaatta tagttgccag actgctgctg atgtatggat gtctgaggct
     1141 aaatgcatca tcggtattga tgttcttctt ttgaatgggc tttatattac aaagtaaata
     1201 agggctgatt ttttcaaaaa cagaatcaac aatagcattg acttcatgca attgattaat
     1261 tgatgtaaaa ccacctaatt tattacgata cgaaactata gctgctgccc tgccaggacc
     1321 aatagcaggc aacttctgcc actcttcctc atcagctaaa ttaatttcaa taggctcaat
     1381 agtccattct ttttttactt taatagaatc ttgctgatat ttattctgag cgaatgtcgg
     1441 cattgaatca ggcagaagaa taaaggaata aatactgtca taaaacgaag gggtgatgac
     1501 aaagagtttt tttacatctg accggattct gaattttcct ccacgctctt catatttttt
     1561 gattgatgca atttgttttt ctgataaacc taacttggtc caaagcgaat cggctaaatt
     1621 atttggattg aaagggaaaa gtattctagt agtttgtgga gaattatttt ctgaaagttt
     1681 cgttgcgtct ttgacaacaa tagaatcggc ctgtaacaaa ttccattgtg ctacagcaga
     1741 atctaaacga cttgtgttga tggcaattgg tggctcataa aatttaagtc cgtacagtgt
     1801 cagcagcagt acaacaataa taatggataa tacgataatg cccttacgtt cactgtaagt
     1861 aaacgtgttg tactgtttaa aaatactccg taggttatga aaaggcggtt tctttttcat
     1921 ggttaaaacg gtaagttaat tttctaaagc gaagatagta ttttaaaaca gagtttgtat
     1981 gcaatggatt tcattttctt gaagcctgat tgattataat gtgggggttt ttaaagatat
     2041 ttgcagaaaa tatttttatc atgtctgtct tgccgccatg tcctttatgt cattcaattt
     2101 atacctatga aaatggtaat ctgatggttt gtcctgaatg tttttacgag tggaatcccg
     2161 aagaacaaca atctgtttca gaagaaacga caagtgctga tagtggaaaa gtttttgatg
     2221 ctaacggaaa tgaattgcaa aatggcgatg cagtaattgt cattaaagat cttcctgtaa
     2281 aaggagcacc caaacctgtg aaagccggca ccaaggttaa gaacattcgt ctggtagaag
     2341 gcaatcataa cattgattgc aaaatagatg gctttggctc aatggcactt aaatcggaat
     2401 ttgtaaggaa ggcataaaat aaaatcagct aaggatttta tgattcattt tcagtgacac
     2461 tttattttct acaataagtt ctgcatagct tcccattgcc agtggattat tttctgtaat
     2521 atgtcctgca gggaaattaa aacaaacagg aaaattgtat tctgaaacat gttcggcaat
     2581 aatttcttct gctgtttttc caaatggaat ggtgttgtct ttcatatcgc tcatagcacc
     2641 aacaacaagt cctgctaaac catgtaactt accactgcgt ttgagctgca tcatcatacg
     2701 gtcaatatgg taaaggtatt catccaaatc ttccaaaaaa agaatttttc cattagtatc
     2761 aatgtcagat gctgatgcag caagtgaata taaaatagaa agattgcctc caaccaattg
     2821 cccagtggca tttcctgttc tgtttaaagg atgtgtggca gcatttaaca caatgctatt
     2881 gccgaaaaga aaatctttta tttgttttaa aataacgtta ggcgatttcg gaaaatttat
     2941 tgccataggt gcatgcagtg tgacaatgtt caactgcttg tgcacatgtg agtgaatggt
     3001 agtgatatcg ctgaagccaa taagccactt tggatttttt ttgaactctg aaaaattaat
     3061 gctgtcaatt attcttactg agccataacc accacgtgca aaaagaacag ctttaatgtt
     3121 tggattatcc aatgcccact gaaaatcttc tgcacgtaaa gtgtcatcac cggcaaactg
     3181 atttttatca gcatataaat ttttaccctc tgtaacatgt aatccccatt gctgaagtat
     3241 gtcaattgcc ggagccattt catcgcggct gactttgcgt gctgtggcca caatggcaat
     3301 ggtatcacct gctttaagcg gtagggggaa aatcatctgc tgttgttttt ttatctttgc
     3361 aaagtaaaaa gaaaagagtt tttagttaga tgttttgcgt ttaaagtttt taaaaaacct
     3421 tccggacttt agctttttaa ctttctagac tttacaactt ttaaaaacaa ttcatgtcaa
     3481 gatatttaat cactgccgca cttccctatg ccaacggtcc ggttcatata gggcaccttg
     3541 ccggatgcta tattcctgcc gatacctatt cgcgctattt gcgacttaaa ggcgaagacg
     3601 taaaatttat ttgtggaagt gatgagcatg gcgtacctat tactatcaaa gcacgacaac
     3661 ttggtattac accacagcaa gtggttgaca aataccatca gatgatgaaa tcgtcatttg
     3721 aaaaattggg catttcattc gatcattatt cacgcacatc ttctcctatt catcatcaga
     3781 cagcatccga tttttttaaa acactttatg acaaaggcgt ttttattgaa gaaactacag
     3841 agcagtttta tgacgaagaa gcaaaacaat ttttagcaga ccgttatatt accggtactt
     3901 gtccgcgttg taataatcca aatgcctatg gcgaccaatg cgagaactgc ggtgcttcac
     3961 tgaacccaac agaccttatc aatccaaaat caaaactgag tggcaacaca cccgttctca
     4021 gaccaacaaa aaactggttt ttgccaatgg gagaaattca gaagacaaaa gaatttcagg
     4081 aatacattaa acgcttcgac aactggaagc caaacattaa aggacaatgt tcaagctggc
     4141 ttaacgaagg attgcatccg cgtgcaatga cacgcgacct cgactggggt gtgaaagtac
     4201 ctttgcccaa tacagaggga aaagtgctat atgtgtggtt tgatgcacct ataggatata
     4261 tatctgcaac aaaagagttt ttaccaaaca actggaaaga ttattggcaa aagcaaaaca
     4321 aagaagaaga aaatcctaag ctcattcatt ttatcggtaa ggacaatatt gtttttcatg
     4381 ccattatttt tccgatgatg ttgatgcagc atggcggctt tgtattgcct gataatgttc
     4441 ctgcaaatga gtttttgaat cttgaaggtg ataaaatttc tacttcgcgc aactgggcag
     4501 tgtggctgca cgaatatctt gaagattttc cgggtcgtga agatgaattg cgttatgtgc
     4561 tgacatctat tgcaccggaa actaaggaca gtgagtttac ctggaaagat tttcaggcta
     4621 gagtaaataa tgaactggga gctattgtag ggaattttgt aaacagagtt gtggtgttga
     4681 gtaataagta ttttaaaggc aacctcacaa ctgatagaga aattgctgct tcaccatttg
     4741 aaaatgaatt gaaaaaatta ttgcagtctt atactaaagc aatggaaaat tttcaattca
     4801 gagatgcaca gtcaatagca atggattttg ctcgaatagg taataagtat ctgacagagt
     4861 gtgaaccatg gaaaaaatat aaagcagatg atgttaccag tgtcaatcaa accagagaag
     4921 tactgattca ttgcctatca ttggtttcaa aaatggctgt gttgtttcaa ccttttttgc
     4981 caaatgcatc agctaaaatg cttgatatgc ttaaggtaga ttctgaaaaa agaaagctta
     5041 ccaaaaatgg ttttgaagac aataaaattt cgttaaacaa tattatttta aatattgaca
     5101 gccttatctt atttcaaaaa atagaagacg ctgacattga aaaacaaatt caaaaacttc
     5161 atgacaaaaa agtgatgaat acagaacaga atcagcaagc agaagttgtt agtgaaacaa
     5221 ccattgcgcc tcaacttgcc gaaactactt ttgatgattt cagcaagatg gatattcgtg
     5281 ttgcaacaat tttagaggca gagcgcgttc caaaaacgga taaacttttg aagttactac
     5341 tcgacacagg cattgataaa cgcactgttg tttcaggtat tgcagcatgg tacgatcccg
     5401 aaaaaattat cggacaaaaa gttgtgatgt tagccaacct tgctccacgc aaaattaaag
     5461 gtattgaaag caaaggaatg atactgatgg cacagaatga taaaggagag ctttcattta
     5521 ttacaccatc aaagccgcag atggacaatg gaaacaccgt taaatagatt taaaatttat
     5581 taattaaaat acaatggaat tcagaaaaga aaaagacacg atgggaattg ttgaagttcc
     5641 tgcaaacgtt tattggggtg cacaaacaca gcgctcaata gaaaatttta aaatagcccg
     5701 cgacacaaat aaaatgccta aagaaattat tgaagcattt gcctatctta aaaaagctgc
     5761 tgcacaaacg aattttgaag caggtgtttt accaaaagaa aaaaatgaat tgatagcacg
     5821 agtgtgcgaa gaaatcctcg aaggaaaatt aaacgatcag tttccacttg ttgtatggca
     5881 aacaggtagt ggtacgcaaa gtaacatgaa tgttaatgaa gttgtagcct accgcgcaca
     5941 tgtcattaat ggtggaagtt tattggacga gaagaaagct attcatccaa atgatgacgt
     6001 aaacaaatcg cagtcaagca acgatacttt tccaacagca atgcatattg ctgcctataa
     6061 aatattgatt gacgtaacac ttccgggaat taaaaaactg agagatacac tcaaagccaa
     6121 gtcgaaggag tttatgaatg tggtaaaaat cggacgtaca cacctgatgg atgccacacc
     6181 gcttacatta ggacaggaat taagtggcta tgtatcacaa ctcgatcacg gtattcgtgc
     6241 tattgaacat acactaccgc atttagcaga acttgcattg ggtggcactg ctgtcggtac
     6301 aggaatcaac acacccaaag gttatgatgt gaatgtagcc aagcacattg catcattaac
     6361 aaggctgccg tttgttactg ccgaaaataa atttgaagca ttggcagcac acgatgccat
     6421 tgttgaagca catggtgcct taaaaactgt tgcctgctca ctgatgaaaa tagccaacga
     6481 cattcgtttg cttgccagcg gcccccgttg tggcattggc gaagtatata ttcccgacaa
     6541 cgagccgggt tcatctatca tgccgggaaa agtaaatcct acgcagtgcg aagccatgac
     6601 aatggtagct gcacaggtgt tgggtaacga tgtagccata aacattggtg gtgcaacagg
     6661 acattttgaa ctgaatgttt ttaagccggt gatgatttat aacttcctgc atagtgcacg
     6721 attgattgga gaagtttgta tttcgttcaa cgacaaatgc gccataggca ttgcaccatt
     6781 acatgacaat atcaaaaaaa atctggaaaa ttcattaatg ctggtaactg cacttaacac
     6841 acacattggt tactacaaag cagcagagat tgcacaaaca gcacataaac aaggcaaaac
     6901 tttaaaacag acagctattg atttgggtta tgttacttca gagcagtttg atgcctgggt
     6961 gaagcccgaa gatatggtgg gattgtaatt tttattactt ttacgattaa ataatagttt
     7021 atgaagaagc ttatcatttt cgcatttatt tttctgactt cggttgtggc atttgcgcag
     7081 tcgggtgatg tgtttaccac agtagaaaaa atgcctgaat ttcccggagg tcaaacagga
     7141 ttgcaggaat atcttcttaa aaacatcaaa tatcccgaag aggcaaggac caatgttgtg
     7201 gaaggcgttt cgtatataaa ctttgtggtg tcatcaacag gagatgttac cggtgcgcgt
     7261 gttttaaaag ggaacaatga gcaatttaat caggaagctt tacgtgtagt tagtgctatg
     7321 cctcgctggc aacccggcat gcagggtggg cagactgtaa gcgtacaata tacattgcct
     7381 gtacgttttt cattaacggt gcctgataaa gaaggtaaaa aacataaaaa atagattaat
     7441 atcatctttt tttgaaacag ctgtcaaaac ttcattgaca gctgttttgt ttttttgtaa
     7501 attgtgctct catattttaa tacaatgaac cgctttaaaa atatcctcat taatggtaaa
     7561 catggtaaac cgattgtaac cgacatgttt ttcaatggca ataaagagcc aatgcctgta
     7621 attatttttg ctcatggctt taaaggcttc aaagactggg ggccttatga tatgattgca
     7681 gaatcgtttg ctcattcagg atttgtcttt attaaattta atttttcgtt taatggaact
     7741 acccccgata acctgactga atttgcagac cttgaagcat tcggacaaaa taattttacc
     7801 aaagaacttg atgatttaga cacactactc aactggcttt ggcagcatcc acataaagag
     7861 cgttttgatt taaacaccat ttatctggca ggacactctc gcggaggagc tatttcaata
     7921 attaaagctg ctgaagatgg tcgcatcagc aaaattgcat catgggcatc gcctattgag
     7981 tttagtaaat atatttcgcc acagcaactt agtttatggc agcaggcggg agtcatttat
     8041 atagaaaatg cacgaaccca tcagcaaatg ccactctact atcaacttgc tgaagatgcc
     8101 atcaacaatg cacaaagaat aaatattcat aaggcagtaa aacagctttc aatacctcat
     8161 ttaatcattc atggaacaga ggatgaaaca gtaagtttta gtgatgcctt agtcatgcat
     8221 tcatggaata caagggcctg tctgacacca ataacatcgg caaaccatag ttttggtgcc
     8281 gagcatcctt atgccggaca attactgcct cctcattacc agcaagttgt aaacgaaacc
     8341 attgcttttt tcagaggctg atatctctaa agatttcgtt ttatttattg cattttcagt
     8401 tgtatattcg ccccctcaat tttaaacccc cattattaag acattatggc tattattgga
     8461 aaaataagaa gtaaggccgg cctgctcatt ggtattatcg gattaagctt agtagctttt
     8521 atccttggcg acttgctcac ttctaaccgc tcattcattc agggaaatac cacaacagca
     8581 ggtatcatag gtggaaagaa aatctctatt caggattttg aagccaaagt ccaacagaat
     8641 atagaaaatt ataaactcag tcagggtaaa gatcagatag accaacagac tacagatcaa
     8701 ttgcgcgatc aaacctgggg acagatgctt aacgaagaat taatcggcaa gcaaattgca
     8761 aaaacaggtt tggtagtgag cagcgatgag ttgtttgaca tggttcaggg taatgacatt
     8821 cacccacaga taaaacaggc attcagcgac ccaaacacca agcagtttga taagaaaaat
     8881 gtaatccagt ttttaaaaaa catggataac gatcagacag gccgcaccag agcacaatgg
     8941 gtaaacttcg aaaacagcat taaagaagaa agactgaatc agaaatattt cgacctgatt
     9001 aaaaaaggcc tttatgttac cactgctgaa gcaaagctcg actttgaagc aaagaacaaa
     9061 actgagtctg cacgttacat catgcttaac tacagtacca ttgccgatag cacagtgaaa
     9121 cttactgatg ccgatttgca aaaagcatat aacgaaaata ttaaaagcta tgagcagccg
     9181 gaagaatcgc gcaaactcga ctatgtgact tttgatgttg tgccttctaa cgaagacaga
     9241 gcagtagcag aaaaatatat tacagacctt gttcctgcat ttactgcaag cacagacgac
     9301 tccttgtttg tagctcagaa ctcagatttt cctgttgaca acagctacca taaaacagga
     9361 acactttctc ctgctattga agcacagatg tttgcagaag ctgtaggttt tgtgtcagca
     9421 ccatatatgg aaaacaatgc ctactatgtt gcaaaattag aaggctcaaa aatgcttcct
     9481 gattctgttc aggcacgcca tattttattt aagacagaag gaaaagattc attacatgtt
     9541 aaagcaactg ccgacagtat ttacaatctg ataaaaggcg gtgctaaatt cgatcagttt
     9601 gctatgcttt ctgaagatcc gggctctgct attaaaggtg gcgacttggg ttggtttaaa
     9661 cccggaatga tggttcctga attcaacgac ttttgctttg acaataaaaa aggctcgtta
     9721 ggtgtagtaa aaacaaactt cggttatcat ataattgaaa ttaccgatca ggcaggtatg
     9781 tcaaaaaatg taagggttta tgctgttgga cgtaaaattg aagccagctc taaaacctat
     9841 cagggtgtat ttgcaaaggc aaatgacttt gcagcaaaaa acaacaatgg cgacagttat
     9901 actaaagctg taaaagacga aggattaaat gcccgtaata ttgaaagctt aaaagaaaat
     9961 gacaagactg ttccaggcct tgaaaatgca cgtgagttaa ttcgctgggc atttaaggct
    10021 aagaagaacg atgtttcaac accatttgag ttgggtgata aatttgttgt agcacattta
    10081 atgcagataa aacctgaagg cacattggca ttggaagatg taaaagaaca ggtagaagtt
    10141 gaggcacgca aaaacaaaaa agcagaaatg ctgattgaga aaattaatgc aacaggttca
    10201 gcacaaagtc ttgacggatt ggcatcaaag ctaaacatcg ctgttcagcc tttggaaaat
    10261 gtaacattta actctccata cattccaaac ttaggtgcag aaatgaatgt tataggaact
    10321 atgtttggaa taggaaaaga caaagtgagc aagccaatta aaggagagca aggtgtattc
    10381 gttgttcagg ttactgatgt aaaagaaccg caggctcctg ctaattttaa tgaaagtaaa
    10441 acgcaggcag agcaacaatt gcaaaaccgt tcgcagtacg aaacctttaa tgcattgaaa
    10501 gaaaaggcaa aggttaaaga ttatagaggt aagttctatt aagaattgta aaaaagattt
    10561 tacttaaaaa aataagggct tcggaacaaa aatctccgaa gcccttaata tttttggggt
    10621 gtgaataaag agaaagtcat attgtggttt aaaaggctgg ggttggcagg atttttattt
    10681 ttcctgatca aaggccttat ctggcttgca gtactttatg gtgcagtaga gtggtttaaa
    10741 taattgtgct taatatagtc aggcatcaac atagttgaga atagacttaa aaattccaag
    10801 tccatctgta tttcccaata atgtgtctgc ggcacgctca ggatgtggca tcattccaaa
    10861 cacatttcgt ttctcattac agatacctgc aatattgttt atggcaccat taggatttgc
    10921 agcttccgtt acatagccat tctcatcaca atagcgaaaa agtacctgat tgttcgattc
    10981 tatctgattt actgttgctt catctgcaaa gaaacgtcct tcgccatggg ctataggaat
    11041 tttaatggct gagcctattt tcagtgatga tgtaaggatg gtttcatcag tctctgcttt
    11101 tataaaaaca tttttacaga taaatttatg gctgttgttg tgcaataatg ctccaggcag
    11161 cagttgcgat tcgcataaaa tctgaaagcc attacaaaca cctaaaacat aaccaccttt
    11221 tgcagcaaac tccatcactt cctgcataat gggcgaaaaa cgtgcaatag cacccgaacg
    11281 caaatagtca ccataagaaa agcctccggg aagtacaata aaatcgcatc ctcgaagtga
    11341 atgttcctta tgccataatt tttctaccgg ttgtttcaat atgttgcgca acacatatac
    11401 catgtcctcg tcacaattgg aacccggaaa taccacaaca ccaaatttca tgattgattt
    11461 ttttactgac tacaaaagta acgataaatt aaactgcttt aacaatcttt ttcagagatg
    11521 tttgttgagc aaattaaact caagaagttt cctgaatgat ttttcacgta cggttttatg
    11581 caatgcctga atgtgtttgg tggcatgtgt gtcggtgcca ataaggtcaa cccattcttt
    11641 ttctataagt ttttctgcaa tacgtttggc atcgggacca tagtacccgc caagagagtt
    11701 tacatttatc tgtaacaaaa caccttgttc gcgcagactg ccatagaagt cgaaattatt
    11761 tccccagaaa ggataacgtt ccggatgtgc cataatgggt ttgtagccca gcacctgcat
    11821 tctgaaaatt acctgtaata aattatcggg agcgtttaca taactgattt caaaaagcaa
    11881 atggttgttg tttccaatgg tcaacaactt ttcttcttct aacttatgta taaatccatc
    11941 gtcaagatag tattcggcag cggcctcaat cgttacagga atgttttctg cctttactgc
    12001 tttgcgcact tcttcaagcc cagccaaaat tgtttcgggc gtattgcgat aaaaatcgct
    12061 catgatatgc ggtgtagtaa tgagctttgt gtagcctaat gcatgcagtt cttttatcag
    12121 cgtaagagag tcttcaatgg tttttgcacc atcatcaatc cccggaatga ggtgactgtg
    12181 catgtcggtt ttaacttcta aaaaattggc cggtgttgaa tcttttcttt taaaaaagga
    12241 gaacataatt tacttcctgt aattaaggca acaaaagtat aagaaaatat tgcaatatac
    12301 attgttatct gtaaggtaat taaacatctt catgtagcag caatgctttt tattcaacag
    12361 gcaaggtaat aataaattct gtcccttttc cattttcaga aatcagcatg atgtcacctt
    12421 tcagattccg gataatatct ctgcagatac ttaaaccgag tcctgttcct tcacctgcag
    12481 gcttggtggt aacaaaaggt tcaaaaattt tggcttgcaa ctctgcagga atgcccatag
    12541 cattgtcttt aatgtgcaga taaatttttt tgtttacatt ctttgtgatt atctgtaatt
    12601 cgggatgata attttcgtta gcaggagaat ttttattcct ttcgttgagt gcataaaaag
    12661 cattatttac aatattcaga ataacgcgtc caagatcctg agcattggta ttaatcattg
    12721 gcagttgttc attatagctt tcaacaatgt tacattcaaa accaaatatg tttgatttta
    12781 tgcctgctgt tgccagatag acatattctt tacaaatagt atttacattg gtaagttcaa
    12841 aatcaccggt gttgccacga ctgaaagaaa caatgcgttt aatgatgcgg cttgcacgca
    12901 tgccatgttg ttgaatcttt tcaatattcc ctgcaacatc attcaacaag gtgtcctttt
    12961 ctgtagtgtt gtttgtgctt tttatttcac cgataatttc cccactcagt tcagcaaagt
    13021 tgttgacaaa gtttaaaggg ttttgcaact catgtgcaat accggcagat aataatccta
    13081 atgaagctaa tttttcctgt ttaatcattt caatagttct gacctcaaca tcatttttag
    13141 cttttgacaa ttctttattt aacctgtcta atcgttggac aaatacagta tgttttaatt
    13201 gattttgtga atacatcttt tgaataaaga caattaaaat agtattaaat gattcatgat
    13261 gttttgaaag cacagcaaaa aaatcttgtc tgtttatttg cagcaagact acatcatcgg
    13321 cagcaacagc cgaagactga tatacgccat caggtaaaac aaactcttca ccaaaacaat
    13381 catttgtttg tagtttcaat aattcaatct cattttcaac aatagagatt gtgcctttct
    13441 ctatgaaaaa aattttattt gccttatcac cggatttaaa tacagtttgg cctttattga
    13501 aatgtacaga agtgctgatg gtagagagta aatgtatcag atcggcagat aaatttttca
    13561 aaaggtcagc ttgagctaac gatgttgcaa ttttattcaa gctgctgact gccatgtgga
    13621 aagttgggta aatgtaataa aatatccacg gtataatcaa cagcagaaat tatggtggag
    13681 ttaattttat ttatgattta tatcatgtct atttaacata tcacactgtg taatgagtat
    13741 agatttataa tttttaaaag caagagttaa ctttttgcta ctgtaatttc aaaatgatgt
    13801 taagccaaat taatgtgttg gaatcaatgc ggtgaaaagt gtcataactg agtgtgccag
    13861 atttttaaaa aggcagaaat acaatcaaca gacaaattat gtagctgaaa actatgcaat
    13921 taatttttta acccaatatt tacaataatt cgccaaacga aaggttttta ttaatgttta
    13981 gtcaagtata ttagcactgt aattttaatc tatgaaacca atgttcgaat acaccaaaga
    14041 aatcctcacc aaggtgagtt ttgataaaaa gc
//
LOCUS       DAOOOL010000066        14044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_377_length_14044_cov_124.257845, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000066 DAOOOL010000000
VERSION     DAOOOL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_377_length_14044_cov_124.257845"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKU82_06795"
     CDS             <1..305
                     /locus_tag="PKU82_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82617.1"
                     /translation="PLFDLLRVFIVRILNKRSPFQADRLHIHHLLLDMGLNHREVSIT
                     LYLVNIGFILVALALRSFGALALLFVISSLALILSLVPYYLKYQAGRSGAVSRSTK"
     gene            453..2012
                     /locus_tag="PKU82_06800"
     CDS             453..2012
                     /locus_tag="PKU82_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOZ82618.1"
                     /translation="MRLKKLVYSIALLLPFIGNAQVVENAQPQSGDPIAAMLDSLVSI
                     RNVVRLSETNLQQPPVFSENDAVLSDAFIQERISKISTPIPLTYNYQVKNYINLYASQ
                     RAQLTSRVMGLSRLYFPYFEQMLDKYNLPVELKYLAIVESALNPTAVSSAGATGIWQF
                     MFNTGKMYGLNINSYIDERRDVRLATDAACRYFQDMYAIYGDWLLVIASYNCGPGNVN
                     RAIARAGGSKNFWEISRFLPAETRGYVPAFIGATYVMYYAKDYNITPTKPSFTYFDVD
                     TVGVKNKISFRQLSQTLNIPYDVIAFLNPVYKRNMIPGEGNRLYLPTEKISMYLANEE
                     GLYAASGTELQPSSFAIADDVGYVQVKQHVKVRKGENLFTFAKRNDCSAKQVKKWNGL
                     RSNNIKAGQKLVVYATIKQKPAPVENTTATAANDKTDSANVNTTVKADSSSDSETGAV
                     TTESKTTQVSQAQSAASKYIIHLVQPGDTLWNIAKRYEGVTVELLKEANNFTPKSTLK
                     VGSKIKVPTES"
     gene            complement(2031..2573)
                     /locus_tag="PKU82_06805"
     CDS             complement(2031..2573)
                     /locus_tag="PKU82_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOZ82619.1"
                     /translation="MSILFHSMNILDLFLAVPLLWGAYKGFTRGIIYEIAMILGLIAG
                     IYVAFKFSGVVQGWLFQLVNKDSALPAWVGFFIISGIIMAVFILYARLLEGILKAGDL
                     NLFNKIIGGVFGLLKYALVLSVFLWLLKSLEPHLNFINSQTKKESKLYDPVLKTSTFL
                     TPALQDIKNEFRQHIEDNNH"
     gene            complement(2649..3572)
                     /locus_tag="PKU82_06810"
     CDS             complement(2649..3572)
                     /locus_tag="PKU82_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOZ82620.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKIIGGAATYASLAASYFAPKIN
                     LVGVVGDDFPQSTIQDFNNHNIRTEGLQIIEGEKSFFWSGRYHTDMNSRDTLDTQLNV
                     LANFDPIIPESYQDCEFLMLGNLMPKIQLQVIERLRKRPKLIVMDTMNFWMDMAMDDL
                     LKNISMIDVLTINDAEARQLTNEYSLVKAAKKILNMGPKYLIIKKGEHGALLFHNNEV
                     FFAPALPLEDVFDPTGAGDTFAGGFIGYLAKTRDISFSSMKRAIIYGSAMASFCVEKF
                     GTERIMNLDSDELNERIQEFIDLVQFDITLV"
     gene            3743..4288
                     /locus_tag="PKU82_06815"
     CDS             3743..4288
                     /locus_tag="PKU82_06815"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOZ82621.1"
                     /translation="MLEQLLNLVKENAGEAIINNQAIPNEQNDAAISQATEGIIGGLK
                     SQLASGNISEVAQFLGGQNNAATTGVTNAISSQVSQGLMEKFGLSGNSAGSIVSSLIP
                     VVLSKLVSKTNDPNDKSFDLNSIFNHLSGGKTQGMDLSNILGSVTGGNASGGGIGNVL
                     GSVMGGDKNSALGGLMDIFKK"
     gene            complement(4429..4863)
                     /locus_tag="PKU82_06820"
     CDS             complement(4429..4863)
                     /locus_tag="PKU82_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOZ82622.1"
                     /translation="MDSIFGSYTLEEVKLMGKNTMLNHIGIEITSIKNNTIKGKMPVD
                     NRTRQPKGIMHGGASCVLAESLASLGAFLTLKDKSATCVGMEINANHVRPVKEGFVYG
                     TAKPFHLGKKTQVWTIEILNEKQQPVCISRMTIAIGRLHLKI"
     gene            complement(4873..5529)
                     /locus_tag="PKU82_06825"
     CDS             complement(4873..5529)
                     /locus_tag="PKU82_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82623.1"
                     /translation="MSSKKIKYTLLTIFVLLSCSGNVHAQLRDTLAYLFKQRPKPFFQ
                     LDGYNSFVRSRGANAIGFKTGLDFGKRVKFSMGYMKLFTDVVDSVQVKPNKYYRGEVR
                     SNYFTTGIEYVIYRNGPWQVNVPMHFGFGTSYYEYPDGKKANNKALQGNIILFEPAIT
                     GHYKLIRWVGLGFGVGYRVMLKNNHQLKDNLSSPIYILRLKIFVDEIYRTVFPPKQKK
                     "
     gene            5731..6963
                     /gene="odhB"
                     /locus_tag="PKU82_06830"
     CDS             5731..6963
                     /gene="odhB"
                     /locus_tag="PKU82_06830"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOZ82624.1"
                     /translation="MKVEIKVPSPGESITEVQIARWIKKDGDYVERDEEIAEIDSDKA
                     TLSLVAEADGVLKTLVGEGENIAVGSVVCTIDTDAKPVAKAEPKKEEKPKEEKPKEVK
                     PTVTVASAKAEGYAAGTPSVAAAKMMKENNVKSESVQATGKGGRITKGDVLQSLQTPA
                     ATQAAPQAREQRREKMTMLRKKLSQRLVAVKQETAMLTTFNEVDMSAIMDIRKKYKDV
                     FKEKHGVGLGFMSFFTKAVCEALKSFPAVNAQIDGEEILYHNYCDVGIAVSAPKGLMV
                     PIIRSAETLSLAQIEQQVIDLATKARDSKLTIDEMTGGTFTITNGGVFGSMLSTPIIN
                     PPQSAILGMHNIVERPVAVNGEVVIRPVMYVALSYDHRIIDGRESVGFLVKVKQMLEE
                     PAKMLFEGNDPIKVLLHL"
     gene            complement(7040..9199)
                     /locus_tag="PKU82_06835"
     CDS             complement(7040..9199)
                     /locus_tag="PKU82_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOZ82625.1"
                     /translation="MNHKLRLFLTAFALFFTTMLHADEGMWLLSTLKQLNEADMQKMG
                     FKLTADDIYNINKSSMKDGIIHFGGGCTGEIVSPDGLILTNHHCGLDYIQSLSSLDHD
                     YLTNGFWATARDKELPAPGLSVKFLIRMEDVTQKVKAEISDTLSEADLQAKLPGIYSK
                     LTKEATAGNEFYTADVRAMYGGNEYFLFVYERYEDVRLVGTPPMSIGNFGGDTDNWMW
                     PRQTGDFSMFRIYTTPDGKPAKYAKENVPLKSKYYFPVSIKGVKEGDFAMIMGYPGRT
                     DRYATSYSIENGLDITSPSLVKLRTKRLDVINNAMADNDAIRIKYQAKRNQVSNYWKY
                     FIGQQKQLKRMKVVEQKRNLENQFTTWVNADKKRRLHYGFVMGNLQQSYDNARKYAKL
                     RVYTSEALMGTEILRYAFSFSALDKALNEKEPNADDIKAKAAMLKSRVEDYFEEYDAG
                     VDQKVFAVLVKSFADDVAKDQQSPIFAEMAKKYKGDYNKMAAAVFAKSIFSDKNKVLA
                     FLENPLAKKLKNDPAFKLINTQLSFYDDNFRAPIRTIDLALAANNRLYIEGLREMMPD
                     KKLYPDANSTMRLSYGSVRAYDPADAVKYSYFTTIEGVMQKMDNSNTEFKVPEKLVEL
                     YNKKDYGRYGSNGTLNTCFLTNNDITGGNSGSPVINGNGEIIGLAFDGNWEAMSGDIK
                     YDEEYKRTICVDIRYVLFCIDKFGGAKHIVDEMKVVE"
     gene            9444..9716
                     /locus_tag="PKU82_06840"
     CDS             9444..9716
                     /locus_tag="PKU82_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82626.1"
                     /translation="MPCTDNDALDIFSDLRHSSQSTSANDDHNHEAETCSPFCICACC
                     GHTAATQFTFLYFTTCTAVSNVQFNPYKILLSTESYIGIWQPPKIS"
     gene            9810..>14044
                     /locus_tag="PKU82_06845"
     CDS             9810..>14044
                     /locus_tag="PKU82_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOZ82627.1"
                     /translation="MLEKIISFSIKNKLIIAMMTLALIIWGVWSAGRLPIDAVPDITN
                     NQVQIITLCPTLAGQEVEQLVTYPIEQSIANLPDLEELRSISRFGLSLITVVFKENVD
                     VYFARQLINERLNEAVENIPAGVGKPEMGPVSTGLGEVYQYIIHPKKESENKYSAMDL
                     RTMQDWIVARQLYGTPGIAEVNSFGGKLKQYEVAVNPSRLLAMGVTIPDIFSALQKNN
                     ENTGGAYIDKKPNAYFIRGIGLIGSFDDIKNIVVKQNPNGIPILIKDVAEVQFESAIR
                     YGALTYNGEKDVVGGIVMMLKGYNSAEVVNRVKEKMSTIQKSLPDDVVIEPFLDRTDL
                     VKRAISTVEKNLIEGALIVILVLVLFLGNLRAGLIVASAIPLSLLFALGMMNVFGVSA
                     NLMSLGAIDFGLIVDGAVIVVESSLYFLEHRKNMLRLTQNEMDDVVENRTKRMMSSAV
                     FGQIIIMIVYLPILSLIGIEGKMFRPMAQTVSFAIIGALLLSLTYIPMMSALFLPKQL
                     SHKKSFNDKMMDFLQSKYTPLLNTAIRFKRAVVASAVVIFAIAVFVFSRMGGEFIPTL
                     QEGDYAFHCILPQGTSLSQSIETSMQCMREIKKFDEVKMVVGKTGSAEVPSDPMPTEA
                     TDMIIVLKPKSEWKRKISYDELADEIMEKLEVIPGVFFEKSQPIQMRFNELMTGIRQD
                     VAVKIFGENMDTLLYYANRVNVVVQKVQGATEPSVERVAGLPQIVIRYNRSQIANYGL
                     SIEDVNHIVSTAFAGGKAGVVFENERRYDLMVRLDSASRNNLDDVGRLLIPVSNGMQI
                     PLTQVADVKLELGPAQISRENGRRRIVVGFNVKDRDVASVVEEIESQLAEKVKLPEGY
                     YYTYGGTFENLQAASKRLMIALPIALALIFVMLFFTFKSAKQALLIYTTIPMSAIGGV
                     FALLLRDMSFSISAGVGFIALFGVAVLNGIVLIGTFNQLKQEGVEDILERIKEGTRSR
                     LRPVLMTATVASLGFLPMALSQSAGAEVQKPLATVVIGGLITATLLTLFVLPLFYLFF
                     SSEKKVKVEGNNFSGVMPIILLILSAMLLPTISSAQTNVTLEQAISLAMKNNLHLQAQ
                     QLNVQSYESLKKSSFELPKTEVNFQYGQYNSIKKDNGINIQQTIPMPGYFVTKARLNN
                     AELKGAQLQQNIAIKQIISQVSSAYYEIVFLQQNEKELQKLDSIYSGFVQAATLRYTS
                     GESNLLEKTTAETKRGEVQLKVIRNNTALKIAYSTLEALLNTSNLIVTPISNFVPLVP
                     DNVLDSATITNNFSYQLMLQQAAIAKRTKSAEAAMALPELNIGYMNQSIIGFQEVNGA
                     DVYFNRSKRFTGFSMGITVPLTFFSNVSKVKSFSFRHQELLKEAENSKIELQSQLQNA
                     FATYLQNLNQYNYFKNTAMPNADAIINTSTLAY"
BASE COUNT         4256 a         2471 c         3009 g         4308 t
ORIGIN      
        1 tgcctttgtt tgatttgctc agggtattta ttgtcagaat tttaaacaag cgttcacctt
       61 ttcaggcaga cagacttcat atccatcacc tgttgctcga tatgggttta aatcaccgcg
      121 aagtttccat tacactttac ttggtgaaca taggctttat acttgtggca ttggcattac
      181 gcagctttgg cgcattggca ttgttgtttg ttattagctc tttagccttg attttgagct
      241 tggtgcccta ctacttaaag tatcaggcag gccgttcagg ggcagtttca aggtcaacca
      301 agtagggcat taatacccta tttgcctcac ttacatgaaa aattgatata attatgtaag
      361 catttcccca aaaagtaaac atttttttta ttttcgtccg aacttgaata acaggcactt
      421 ttccggactc tctgcctgct aaattaatta tcatgcgact aaaaaaactg gtttactcca
      481 ttgctctttt attgccgttc ataggtaatg ctcaggtagt tgaaaatgca caacctcaat
      541 ccggtgaccc tatagctgcc atgttggaca gtttggtgtc tattcgtaat gttgtgcgac
      601 tgagcgaaac aaaccttcag cagcctcctg tttttagtga gaacgatgct gtgctttctg
      661 atgcttttat tcaggaaagg atttcaaaaa tttcgacacc tataccgtta acctataatt
      721 atcaggttaa aaactacata aatctttatg cctcacaacg ggcacaactg acttcacgtg
      781 taatgggttt atcgcgtttg tactttccgt attttgaaca aatgctggac aagtacaatc
      841 ttcctgttga attaaaatat ctggcaattg ttgaatcggc acttaacccg acagcagtat
      901 cgtcagcagg tgcaacaggt atttggcagt ttatgttcaa caccggaaaa atgtatggcc
      961 tgaacattaa ctcctacatt gatgagcgca gagatgtgcg tttggcaaca gatgcagcat
     1021 gtcgctattt tcaggatatg tatgctattt atggcgattg gttgctggtg attgcatcgt
     1081 acaactgtgg gccgggaaat gtgaatcgtg ctattgcgcg tgcaggcggc agcaaaaact
     1141 tttgggagat aagtcgtttt cttcctgcag aaacaagagg ttatgtaccg gcttttattg
     1201 gtgctactta tgtgatgtat tatgctaaag attataacat cacgcccaca aaaccttcat
     1261 tcacttattt tgatgtggac actgttggtg taaaaaataa aatttcgttt cgtcagttga
     1321 gtcagacgct aaacatacct tatgatgtga ttgcattttt gaatcctgtt tacaaacgca
     1381 acatgattcc cggtgagggc aacagacttt atctgccaac agaaaagata agtatgtacc
     1441 tggctaatga agaaggtctt tatgctgcaa gtggcacaga gttgcaacca tcatcgtttg
     1501 ctattgctga tgatgttggt tatgtgcagg tgaaacaaca tgtaaaagtt cgtaagggcg
     1561 aaaatctctt cacctttgca aaaagaaatg attgctctgc caaacaagta aagaagtgga
     1621 atggtttaag gtcgaacaat attaaagcag gacagaaatt agttgtctat gcaacaataa
     1681 agcaaaaacc tgcaccggtt gagaatacca ctgcaactgc tgcaaatgat aaaacagact
     1741 cggctaatgt aaatacaaca gttaaagcag attcatcatc tgactctgaa acaggtgctg
     1801 ttacaactga gagcaaaaca actcaggtaa gccaggcaca aagtgctgca tcaaaataca
     1861 ttattcattt agtgcagccg ggtgatacac tttggaatat tgcaaaacgc tatgaaggtg
     1921 ttactgtaga gctattgaaa gaggcaaata attttacacc taaatcaaca cttaaagtag
     1981 gaagtaaaat aaaagtccct acagagagtt aattttcaat ttcggttttt ttaatggttg
     2041 ttgtcttcaa tatgctgacg gaattcgttt ttaatatcct gcagtgcagg tgtgaggaat
     2101 gttgaggttt ttaggacagg atcatagagt ttagattctt tttttgtctg actattgata
     2161 aagttgagat gtggttcgag agatttgagc agccacagga acacactcag caccaaagca
     2221 tatttaagca aaccaaatac accaccaata attttattga aaagattgag gtcgcctgct
     2281 ttgagaatac cttcgagcag acgggcataa agaataaaca ctgccataat gattccgcta
     2341 ataataaaga agcctaccca tgcaggcagg gcactgtctt tgtttactaa ttgaaagagc
     2401 cagccttgca caacgcctga aaatttgaat gcaacataga ttccggcaat gagaccgagt
     2461 atcatggcaa tctcataaat aatgccgcga gtgaaacctt tgtatgctcc ccataataag
     2521 ggcacggcta aaaacaaatc gagaatattc atgctatgga atagtatgct caaagtaagc
     2581 gatgctgttg taatctacaa acagcatcag cagaaaatac caaacaataa attgttgaaa
     2641 ggtgttggtt aaaccaatgt aatatcaaac tgaaccagat caataaactc ctgaatgcgt
     2701 tcgttgagtt cgtcactatc gaggttcatg attctttctg tgccaaattt ttctacacag
     2761 aaagatgcca ttgcagaacc gtagatgatg gcacgtttca tgctgctgaa agagatgtcg
     2821 cgggtttttg caagatagcc aatgaagcca ccggcaaaag tgtcgccagc acctgtgggg
     2881 tcgaacacat cttctaatgg cagtgcagga gcaaaaaata cttcgttgtt gtggaagagg
     2941 agtgcgccat gttcgccttt tttgatgatg agatattttg gtcccatgtt tagaattttc
     3001 ttggcagctt ttaccaaaga atattcgttg gtgagttgtc gtgcttcggc atcgttgatg
     3061 gtgagcacat caatcattga aatgtttttg agcagatcgt ccatggccat atccatccag
     3121 aagttcatgg tgtccatgac gatgagtttt ggacgtttgc gcaaacgctc tattacctgc
     3181 aactgaattt tgggcatgag gttaccgagc atgaggaatt cgcagtcctg ataactttcg
     3241 ggaataatgg ggtcgaagtt ggcaaggacg ttgagttgtg tatctaaggt gtcgcggctg
     3301 ttcatgtctg tatgatagcg gccactccag aagaaagatt tttcgccttc gataatctgc
     3361 agaccttctg tgcgtatgtt gtggttgtta aagtcctgaa tggtgctttg cggaaagtca
     3421 tcgccaacaa cgccaacaag atttatttta ggagcaaagt aggaagctgc aagtgaggca
     3481 taggtggcag caccaccaat tattttatcg gtttttccga agggtgtttc tattgcatcg
     3541 aaggcaacgg ttccaacaac gagtaatgac atgatgtgag tttgaaaatt ttgcgcaaag
     3601 ctaatactat tatcggtttt tgtgattgtg ttttgtaaat gagttgttgc ggcaatgcaa
     3661 ttacacgagc taatatttct tcattaaaaa aataaggaga tagatgtatc tttaaacctt
     3721 aatcttttaa tacataaaaa tcatgttaga acaattatta aatcttgtaa aagaaaatgc
     3781 cggagaggct atcattaaca atcaggctat tccgaatgaa cagaatgatg cagctattag
     3841 tcaggctacg gaaggcatta taggcggatt gaaatcgcaa ttggcatcgg gcaacattag
     3901 tgaagtagca caatttttag gcggacagaa taatgcagca acaacgggtg ttacgaatgc
     3961 catttcatcg caggtgtcgc aggggttgat ggaaaaattt ggtttgtcgg gaaacagtgc
     4021 aggttctata gtgtcgtcac tgatacctgt tgtgttgagt aagttggttt ctaaaacgaa
     4081 tgatcctaac gataagagtt ttgatttgaa tagtattttt aatcatttga gtggtggcaa
     4141 aacacaggga atggatttgt cgaacatatt gggttctgtg actggtggca atgcaagcgg
     4201 aggcggaata ggtaatgtgc ttggctctgt gatgggtgga gataaaaatt ctgcactagg
     4261 tggactgatg gatattttta agaagtaagg aaaaacacct tagtatcaac attaattatt
     4321 aatcccttcg gttttatcaa attgatttta ctgaagggat tttttaattc taaattttta
     4381 aatctacacc caaatatttc aactttcaaa cattccaact ttcaaaactc aaatctttaa
     4441 atgcaaacgt ccgatggcaa tggtcattct gctgatgcaa acgggttgct gtttttcgtt
     4501 tagaatttct attgtccaga cttgtgtttt ttttccaagg tggaagggtt ttgcagtacc
     4561 atagacgaag ccttctttaa caggtcgcac gtggttagca tttatttcca tacccacaca
     4621 ggttgcgctt ttgtctttga gtgtgagaaa tgcacccaag cttgccagcg actctgcaag
     4681 aacacatgat gcaccaccat gcatgatacc ttttggttgc cgtgtgcggt tgtcaacagg
     4741 cattttgcct tttatagtgt tattttttat ggaggtgatt tcgatgccta tgtggttgag
     4801 cattgtgttt tttcccatca gttttacttc ctccagtgta taactgccaa aaattgaatc
     4861 catattttat ttttattttt tttgtttagg tggaaaaacc gtgcggtaga tttcgtccac
     4921 aaaaatttta agtcgcagaa tgtatattgg tgatgataga ttgtctttta gctgatggtt
     4981 gtttttgagc atgacacgat agcccacgcc aaagcccagg cccacccaac ggatgagttt
     5041 atagtgtccg gtgatggcag gctcgaagag aatgatgttg ccctgcaaag ctttgttgtt
     5101 tgcttttttt ccgtcaggat attcgtagta gcttgtgccg aagccaaagt gcatgggtac
     5161 gttgacctgc caggggccgt tgcggtagat tacatattca atgcctgttg taaaataatt
     5221 acttcgcact tcgccacgat agtatttatt tggttttact tgaacgctgt caaccacatc
     5281 agtaaacagt ttcatgtagc ccatgctgaa ttttacacgt ttgccgaaat ctaaacctgt
     5341 tttgaaaccg atggcattgg caccgcggct gcgaacgaag gagttgtagc cgtctaactg
     5401 aaaaaaaggt tttggtcttt gtttaaataa ataggccaac gtgtcgcgta gttgtgcatg
     5461 cacgttgcca ctgcatgata acagaacgaa tatggtgagc agcgtgtatt ttattttttt
     5521 ggaagacatg gttgagccga cagaacaaaa atataaaggt gtgttgtttt atttctgaaa
     5581 tattaatttg cttactaaca attgcaattg aattacggaa ctgatgcagg tcagtgcgta
     5641 tgaaggcaga agtgtgttta tcttttataa ctttgcacaa agagtgtttg tcaggattta
     5701 taaaaatgct gagttttaaa aaagtaaata atgaaagtag agattaaagt tccaagtccg
     5761 ggtgagagca ttacggaagt acagattgca cgctggataa aaaaagatgg cgattatgtt
     5821 gagcgcgatg aagagattgc agaaatagat tcggacaagg caacgctgtc gttagttgcc
     5881 gaagctgatg gtgttttgaa aacccttgtt ggcgaaggag agaatattgc agttggctct
     5941 gttgtgtgta cgatagatac agatgcaaag ccggttgcaa aagcagaacc taagaaagaa
     6001 gaaaagccaa aggaagaaaa accaaaagag gtaaaaccta ctgtgacagt tgcatcagca
     6061 aaagcagaag gctatgctgc aggcacaccg tctgttgctg ctgctaagat gatgaaagaa
     6121 aataatgtga aaagtgaaag tgtgcaggca acaggtaagg gcggacgtat tacaaaagga
     6181 gatgtgttgc agtcgttaca aacacctgct gccacacaag cggcaccaca ggcacgtgaa
     6241 caacgcagag aaaaaatgac aatgctgcgt aaaaaacttt cgcagcgttt ggttgcagta
     6301 aaacaggaaa cggcaatgct tacaactttt aacgaggttg atatgagtgc cattatggac
     6361 atcagaaaaa aatataaaga tgtttttaaa gaaaagcatg gagtaggttt aggctttatg
     6421 tcgtttttta cgaaagctgt ttgcgaggcg ttaaaatctt ttcctgcagt gaatgcacaa
     6481 atagacggag aagaaatttt gtatcacaac tattgcgatg tgggtattgc agtgagtgcg
     6541 ccaaaaggat tgatggttcc gattatccgc agtgcagaaa ctttgtcgct tgcacagata
     6601 gaacaacagg tgatagacct tgctaccaaa gcccgcgaca gtaagctgac gatagatgaa
     6661 atgacgggag gaacttttac gataactaat ggtggtgtgt ttggttcaat gctttcaaca
     6721 ccaattatta atccgccaca gagtgccatt ttaggaatgc ataatattgt tgagcgtcct
     6781 gttgcagtga atggagaggt agttattcgt cctgtaatgt atgttgcatt atcttatgat
     6841 catagaataa ttgatggcag agagtctgtt ggatttttag ttaaagtgaa gcagatgctt
     6901 gaagagcctg caaaaatgtt gtttgaaggc aacgacccaa ttaaagtatt actacatttg
     6961 taaccacttt tcttttacca ataaacaaaa agaggctgcc tgaatcaggc agcctctgct
     7021 attttatttt gtgatgtagt tactccacaa ctttcatttc gtcaacaatg tgtttggctc
     7081 caccaaattt gtcaatgcaa aacaaaacat atctgatgtc aacacaaata gtgcgtttgt
     7141 attcttcgtc atatttaata tcgccactca tagcttccca gttgccatcg aatgcaagtc
     7201 cgataatttc gccattacca ttgataacag gacttccgct gttgccacct gtaatgtcgt
     7261 tgtttgtgag gaagcaggtg tttaatgttc cgttgctgcc atagcgaccg tagtcttttt
     7321 tgttatacag ctcaactaac ttttctggaa ctttaaattc tgtattgctg ttatccattt
     7381 tctgcatcac accttcaatg gttgtaaagt agctgtactt aacagcatcg gcaggatcgt
     7441 aagcacgcac agagccatag cttaaacgca tggtgctgtt ggcatcggga tagagttttt
     7501 tatcaggcat catttcgcga aggccttcaa tatacaatct gttgttggca gctaaggcta
     7561 aatcaatggt gcggatagga gcacggaagt tgtcatcata aaaactcaac tgtgtgttta
     7621 tgagtttaaa tgctggatcg tttttaagtt tctttgctaa aggattttct aagaatgcaa
     7681 gcactttgtt tttgtcggag aaaatacttt ttgcaaaaac tgcagcagcc attttgttgt
     7741 agtcgccttt atatttctta gccatttctg caaagatggg tgactgctga tcttttgcaa
     7801 cgtcatcggc aaacgatttt acaagaacgg caaaaacttt ttggtcaaca ccggcatcgt
     7861 actcttcaaa ataatcttct actcttgatt taagcattgc tgcttttgct tttatatcat
     7921 cggcatttgg ttccttctcg ttaagcgctt tgtctaaagc agaaaacgaa aatgcataac
     7981 gcaaaatttc tgttcccatt aatgcctctg aagtataaac acgcagcttg gcatattttc
     8041 tggcattgtc ataagattgc tgaaggttgc ccatcacaaa tccatagtgc agtcttcttt
     8101 ttttatcggc attaacccat gtagtgaatt gattttcgag gttgcgtttt tgttcaacaa
     8161 ctttcattct ttttaattgc ttctgctgac cgataaaata tttccagtag ttgcttacct
     8221 gattgcgctt agcctgatat tttattctta tagcatcatt atctgccatt gcattgttga
     8281 tgacatctaa tcttttggta cgaagtttca ctaacgatgg acttgtaatg tcaagaccat
     8341 tttcaatgct gtatgaagtt gcataacgat cggtgcgacc ggggtagccc ataatcatag
     8401 caaaatcgcc ttctttaaca cctttaatgc tgacaggaaa atagtatttg gattttaacg
     8461 gaacattttc tttagcatac tttgcaggtt ttccatcggg agttgtgtag atacgaaaca
     8521 tagaaaaatc gcctgtctga cgtggccaca tccagttgtc ggtgtcgcca ccaaagttac
     8581 caatgctcat tgggggtgtt ccaaccaaac gcacatcttc gtatctttcg taaacaaaaa
     8641 gaaaatattc attgccacca tacattgcac gcacatcggc agtataaaac tcattacctg
     8701 cagttgcttc tttagtcagc ttggaataga ttccaggtag tttggcttgt aaatcagctt
     8761 cggaaagtgt gtcgctgatt tctgccttta ctttttgtgt aacatcttcc atgcggataa
     8821 ggaattttac tgaaagacct ggtgcaggaa gttctttatc gcgagctgtt gcccaaaaac
     8881 cgttagtcaa ataatcatga tctaaactac tcaacgattg aatgtaatca agtccgcagt
     8941 gatggttggt aagaattaat ccatcgggcg aaacaatttc gcctgtacat ccaccaccaa
     9001 agtgaatgat accatctttc atggatgatt tgttgatgtt gtaaatgtca tctgccgtga
     9061 gcttgaagcc cattttttgc atgtctgcct cattaagttg ttttaaagtt gacagcagcc
     9121 acataccttc atcggcatgc aacattgtag taaaaaacag tgcaaaggct gttagaaaca
     9181 gtcgtaattt atgattcata atttaaaaga ttttgtgtgg cgaatatctg tatttttatt
     9241 cttataatcg ttaaaaactt gttaatgaca gaggaatatt agacggtaac aaatttattc
     9301 tgttagttcc aaaattttcg catacatttt atgacacaaa tcagttgttt tcttcagtaa
     9361 ccattcattg ggtttttcgt aaatttgacc tgttgaaaat agcagcctac atattcagta
     9421 tatacttttt agctttatct gtaatgccat gtacagataa cgatgcatta gatatttttt
     9481 cagatttgcg tcattcatca caatcaactt ctgcaaatga tgatcataat catgaagcag
     9541 aaacctgctc accattttgt atttgtgcat gttgtggtca tacggctgct acacaattta
     9601 cgtttttata tttcaccacc tgcactgcag taagtaacgt gcagtttaat ccctataaaa
     9661 ttttactttc tacagagagt tatataggaa tctggcagcc gcccaaaatc agttaatata
     9721 aatttatgac attgcttttt gcaatgtgaa ttaatgctat tgcaaaatgg catacaataa
     9781 gttatatgtt taattttaac tgattatata tgttagaaaa aattatttcg ttcagtataa
     9841 agaacaaatt aatcattgcc atgatgacgc tggcactcat aatatggggt gtgtggagtg
     9901 ctggcagatt accaatagat gctgttcctg atattaccaa caatcaggta cagataatta
     9961 cattatgccc tacacttgcc ggacaggagg tagagcagtt ggtgacttat ccgatagaac
    10021 aaagtattgc aaaccttcct gaccttgaag agttgcgaag tatttcgcgc tttggtcttt
    10081 cgttgattac agtagttttt aaagaaaatg tagatgtgta ttttgcacga cagctcatca
    10141 atgaaagatt gaatgaggct gttgagaata tacctgcagg tgtaggcaag cctgagatgg
    10201 gtcctgtaag tacaggattg ggagaagtgt atcagtacat tattcatcca aaaaaagaaa
    10261 gcgaaaacaa atattcagca atggacttgc gcaccatgca ggattggatt gttgcacgac
    10321 agctatacgg cacaccgggc attgccgaag taaattcttt tggaggaaaa ctaaaacagt
    10381 atgaagtagc tgtaaatcct tcgaggttac ttgcaatggg tgttaccata ccggatattt
    10441 tttcggcact ccaaaagaat aatgagaaca caggaggggc atatattgat aaaaaaccaa
    10501 acgcctattt tattcggggt attggtttaa taggttcgtt tgatgatata aagaatattg
    10561 tagtcaaaca aaatccaaat ggtattccaa ttctgattaa agatgtagcc gaagttcagt
    10621 ttgaaagtgc aattcgctat ggtgcattga catataatgg agagaaagat gttgttggtg
    10681 gcatagtgat gatgctaaaa ggctacaaca gtgcagaagt agtgaatcgt gtaaaagaga
    10741 aaatgagtac tatacagaaa tcattgcctg atgatgtagt tattgaacca tttcttgatc
    10801 gcaccgattt ggtaaaaaga gcaatttcta cagtagaaaa gaacctgatt gaaggggcat
    10861 taatagttat tcttgtgttg gttttgtttc ttggaaatct aagagcgggt ttaattgtag
    10921 ccagcgctat tcctttgtca ctgttgtttg ctcttggaat gatgaatgtg tttggtgtta
    10981 gtgcgaatct gatgagcctt ggagcaattg acttcggact tattgttgac ggtgcagtaa
    11041 ttgttgttga atcttcgctt tattttctgg agcatagaaa aaatatgcta cgacttacac
    11101 aaaatgaaat ggatgatgtg gtggaaaacc gcaccaaaag aatgatgagc agtgcagttt
    11161 ttggtcagat aattattatg attgtttacc tgcccatact tagtttaata ggcattgaag
    11221 gaaaaatgtt caggccaatg gcgcagacag tttcatttgc cattattggg gcgctgctac
    11281 tttcactcac ctacattcca atgatgagtg cattatttct acccaaacaa ctttcgcaca
    11341 aaaaatcttt caacgacaaa atgatggatt ttttacaaag taaatatacc ccgttgctta
    11401 acactgctat tcgttttaaa agagctgtgg ttgcaagcgc tgttgtgata tttgctattg
    11461 ctgtttttgt cttttcaaga atgggtggtg agtttattcc aacacttcag gaaggcgatt
    11521 atgcttttca ttgcatatta ccacagggca catcgttatc acaaagcata gaaacttcaa
    11581 tgcaatgtat gcgtgaaatc aaaaagtttg atgaagtgaa aatggttgta ggcaagacgg
    11641 gtagtgcaga ggtgccttca gatccaatgc ctactgaagc tacggatatg attattgtac
    11701 ttaaacccaa aagtgagtgg aaaagaaaaa tcagttatga tgagcttgca gatgaaatta
    11761 tggagaagtt ggaagttatt cccggagttt tttttgaaaa aagccaacct atacaaatgc
    11821 ggtttaacga attgatgaca gggataagac aggatgttgc agtaaaaatt ttcggagaaa
    11881 atatggacac attactttat tatgccaatc gagtaaatgt agttgtacaa aaagtacagg
    11941 gagcaacgga gccaagtgtt gaacgtgttg caggattgcc tcagatagtc atcagataca
    12001 atcgttcgca gatagcaaac tatggattgt caatagaaga tgtaaaccac attgtgagca
    12061 cggcatttgc tggtggtaaa gccggagtgg tttttgaaaa cgaaagacgc tatgacctga
    12121 tggtgcgttt agacagtgca agcagaaata atctggatga tgttggcagg ttacttatac
    12181 cagtcagtaa tggtatgcaa attccattaa ctcaggttgc tgatgtaaaa ttggaattgg
    12241 gccctgcaca aatcagtcgc gaaaacggac gaagaagaat agttgttggc tttaatgtaa
    12301 aagaccgcga tgtggcaagt gttgttgaag agatagaatc gcagcttgca gaaaaagtaa
    12361 aattacccga aggatattat tatacctatg gcggaacatt tgaaaatctt caggcagcaa
    12421 gtaaaagatt gatgattgct ttgcctattg ccttagcact catttttgtt atgttgtttt
    12481 ttacgtttaa gtcggccaag caggcattgt tgatttatac aaccataccc atgagtgcca
    12541 ttggtggtgt ttttgcattg ttgttgcgcg atatgtcttt tagtatttct gccggtgttg
    12601 gctttattgc cttgtttggt gtagctgtgc taaatggaat tgttctcatc ggcactttta
    12661 atcagttaaa gcaggaaggt gttgaagata ttctggagcg aataaaagaa ggaacaaggt
    12721 cacgacttcg tcctgtattg atgactgcta ctgtggcatc gttaggattt ttgccaatgg
    12781 cattgtcgca aagtgcagga gcagaagtgc agaagccttt ggcaacagta gttattggcg
    12841 gtcttatcac tgcaacactg cttacacttt ttgtgctacc cttattttat ttgttttttt
    12901 cttcagagaa gaaagttaaa gttgaaggaa ataatttttc aggagtaatg cctataattc
    12961 ttttgatatt atcagctatg ttgcttccta caatatcatc agcacaaaca aatgttacat
    13021 tggaacaagc catttcatta gcaatgaaaa acaatttaca tcttcaggca caacaattaa
    13081 atgtacaatc gtatgagtct ttaaagaagt caagttttga gttgccaaag acagaagtaa
    13141 attttcaata cggacaatac aacagtataa aaaaggataa tggaataaat atacaacaaa
    13201 ccattccgat gcccggatat tttgttacaa aagcacgatt gaacaatgct gagttaaaag
    13261 gagcacaact acagcagaat atagcaatca aacaaattat ctctcaggtt tcgtctgctt
    13321 attatgagat tgtcttttta cagcagaatg aaaaagagct acaaaaattg gacagcattt
    13381 attcgggctt tgtacaggca gcaacattgc gttacacttc cggtgaaagt aacctgcttg
    13441 aaaaaacaac ggcagaaacc aaacgtggtg aagtgcaatt aaaagttatt agaaataata
    13501 cagcacttaa aatcgcttac tcaacattag aagcactttt aaatacttca aatcttattg
    13561 tgacaccgat aagtaatttc gtgcctcttg tacccgataa tgttttggat tcagctacga
    13621 ttacaaacaa tttttcttat caactcatgt tacaacaagc agcaattgct aaaagaacaa
    13681 agtctgcaga agcagccatg gcactacctg aattgaatat tggttatatg aatcaaagta
    13741 tcattggctt tcaggaagtg aatggagcag atgtttattt taatagaagc aagcgattca
    13801 cagggtttag catgggcatc actgtaccac tgacattctt tagtaatgtc agtaaagtaa
    13861 aatcattttc attcagacat caggaattgc ttaaagaggc ggagaacagt aaaatagagt
    13921 tgcaaagtca gctacagaat gcatttgcca catatctgca aaacctgaat cagtacaatt
    13981 attttaaaaa cactgcaatg ccaaatgccg atgcaataat caacacatca acattagcat
    14041 acag
//
LOCUS       DAOOOL010000067        14015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_378_length_14015_cov_127.337536, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000067 DAOOOL010000000
VERSION     DAOOOL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_378_length_14015_cov_127.337536"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="PKU82_06850"
     CDS             <1..1628
                     /locus_tag="PKU82_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOZ82628.1"
                     /translation="PYTIGKAITNSQVYILDKNRRPVPVGIAGELYMAGDGLARGYLY
                     KPEMTEERFVANPFVPGQRMYRTGDLGKFMSDGNIEFLGRIDNQVKIRGFRIELGEIE
                     SMLRLRKEIKELVVMAREDQPGNKILVAYLSTNAGKQIDVNELREYLRTELPEYMIPS
                     VFMILEYIPLTPNGKIDRRALPVPEVEVKAESANFEEPHNPLESLLVGIWSEVLGIDK
                     IGIHDNFFELGGHSLLGIRMMGAIEQKTGVKLDYPTLFRASTISQLTKVMNAEELNID
                     WPIVVPLQPNGKERPLFFVHMHNGNIQRWRVLLKHLDKDQPIYAIQPRGLDEKYDYHY
                     SVEAMASFYIDEIKKIQPVGPYRIAGLCFGGTTAFEMARQLKAKGETAELVFMINNYA
                     PPSNPMQYKIRETWDEFKGMTLEDKLEFALEKTKNAGKKVLSKLTKVFDSGSTTASEV
                     KVNTKPDIRWVNSVALLNYQPEGEYSEKVVLIKTGEEVAKHYDETLGWKRLISGEIEM
                     HQITGSDNDTIITHKEYYTQLAQYLNQALNQIK"
     gene            1637..2347
                     /locus_tag="PKU82_06855"
     CDS             1637..2347
                     /locus_tag="PKU82_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOZ82629.1"
                     /translation="MDLQLTPEQVFCNELLIQDNDCLVFIFCSDSFIAVESELKKLLS
                     NDETERLLKFKFEKDRQTYCIAHGLKRVLLAALLNTKEQVEISTHSNGKPFINHKKHN
                     MPLHFSLSHSEKMIALAFQKNSPLGVDIEKIQPADKYGNVIQHYFTSDEQKLISRSDN
                     DIFFKIWTQKEAIGKATGQGIANLPEIVIPDDYELSTASLGGYILSTCFFRGNEISFK
                     TIHAQDVLNQINILSLRP"
     gene            2414..4081
                     /locus_tag="PKU82_06860"
     CDS             2414..4081
                     /locus_tag="PKU82_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ82630.1"
                     /translation="MNKTSFHELSLSHEMLQAIDEMGFTEASPIQAQAIPVLMQGQDV
                     IGQAQTGTGKTAAFGIPLLEMLDPKVKATSALVMCPTRELAVQVAQEIKKLAKYKKGI
                     AVLAVYGGEQIQKQINALKRPVHVIVGTPGRIMDHLDRRTINLSKIQMVVLDEADEML
                     NMGFREDMEAILQHAPDERQTVLFSATMPKPILEITKRFQTNPKLVKVTKQELTVSSI
                     EQIYYDIPSSQKADVITQLVELHNLKLMLVFCNTKRKTDEVTEQFQQLGYKAEAIHGD
                     LRQNQRNNVLARFKKGDVNMLVATDVAARGIDVENVDAVFNFDLPLDEENYVHRIGRT
                     GRAGRSGKAFSFVSGRNDMAKIREIEDYSKVRIERHQLPSGKERIELAKIKLAARLKV
                     LIEEGDMEQYDNMFATFKQEGLTLHQLAAALLKMHFAPMLQQSQNKSNSKSSSREERF
                     GREERSGRGNRDSGGGKGKRGKHSGEADMIRLFLSVGKKDKVSKGDIVGAVSGLSNMS
                     SSNIGVIDIYDKFSFVEVNGEFLNDVLSNVNGNKIKGRKVNVEIAKD"
     gene            complement(4083..4664)
                     /locus_tag="PKU82_06865"
     CDS             complement(4083..4664)
                     /locus_tag="PKU82_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOZ82631.1"
                     /translation="MQIVFSFILNEINSNYQLYVVQANYPSEKESRKTFQKELNTKLL
                     KQVTKNPSLQLHYQETGKPYIDGNNAISFSHSGNYTALMITQSKSAGIDIEMTDRNIE
                     AGIDYFLNTNEIAFLSQQKKNLHALTCWCIKEAAIKYFNVSGIDFKNCIHIQPFELLN
                     LGMPTVLIQHSQEEMIKICYRREKDFLLAYTVD"
     gene            4711..5397
                     /locus_tag="PKU82_06870"
     CDS             4711..5397
                     /locus_tag="PKU82_06870"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ82632.1"
                     /translation="MTSPEVFQFGIPVTSVIDSINITGQEIAFYRITNIDALYSKLSS
                     LKNDDDNVIDERIPYWAELWPSAIALAEYLCTRKLNNDMLIHEIGCGLALPSLVCGKM
                     GYSVMMSDYVDAPLQFASANWRLNVKSEPTTILLDWRKIPDDIEKCDLLLAADVAYES
                     RMFPAIVNAFKRLVKSGGRIVFTEPNRKFASGFFELIRHEFTNTTIQNREVIQNNHKH
                     QIKIVEIEVE"
     gene            complement(5415..6617)
                     /locus_tag="PKU82_06875"
     CDS             complement(5415..6617)
                     /locus_tag="PKU82_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ82633.1"
                     /translation="MKILQLCLRIPLPPYDGATIAMYNLAESLTASGAEVTMLSFNTK
                     KHFTDINSIDAALLEKYKLETVFLDASVNAWDAFANLFKPNESYNIVRFDSEAFHQKL
                     KELLQSETFDFILFEGLFLSPYLQTVRTFSKAKCILRAHNVEWLIWKRLAKATKNPLK
                     KTYLYFLSDRLKHYENIAINKFDAIIAISQPDKNLFLKDGCNIPIEVAPTGMNTAKYK
                     NIEQMDADSLSLFHLGSMDWLPNIEAVDWLLKDIWPLINQQSKKIKLHLAGKNMSPKY
                     FNLKSENLIIEGEIKDSKKFMQDKQIMVVPLLSGGGIRIKILEGLAAGKVIVTTSIGA
                     EGINYTDKKNILIADTPQQFADTILALLNNPEKLNSIAKEAQLLARSQYNNNIIGEQL
                     VRFYQTIR"
     gene            6770..7180
                     /locus_tag="PKU82_06880"
     CDS             6770..7180
                     /locus_tag="PKU82_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82634.1"
                     /translation="MQPTKLKSGTVCGNLLAALLLFSTSSIAQPSDAYVSMKATAVAG
                     TVHGVYYIELADTNSVNQIEIKLGTDWTSADMVNAVFTYDTPGSLPQGYSYTRNGNII
                     AIDMGNFPEQSSYYGNVRVKNGATWSNNYTFVSN"
     gene            7235..>14015
                     /locus_tag="PKU82_06885"
     CDS             7235..>14015
                     /locus_tag="PKU82_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82635.1"
                     /translation="MKRIYSLLIYIVFINTFPALATTYTWTGNTSTAWNTTSNWSPSG
                     TPTTGDFVHIYSATYNPQLTGNLTLNGIYIHSGTLNLNGDTLLLNSGTSTFSGGTISN
                     GLLQLRGTEITFNSTTFNAPIDAVVDRINLNGCTFNNYCYFETISNVPGTGSGGAVFN
                     DSVYLYHNYTGSGIWVMNNNTGHRFNGKVTIHNKGTKEITFTGAGINVFNGDIVLINT
                     GAGGIMLGNDTLTAGHTISIGSGGFSAGDFNATNFYQDGNTAISLTFTGTALPSITGA
                     TFGGNFSITSNVLLFKNCTFSGTASFTATGSGSSSWYGGNTFYGNATFTNNATAGNLR
                     IDADTANTYYADATFNGSRELRVCYGGEVSSFYGNITTANNTTFNAGTGTLCLAGSGT
                     QALSFHSNTLVKNLMLNKSGGSAALSTALVVSGTVTFTKGTLTTTSSNLLTINNGGTV
                     SGASDSSFIDGPVKKIGNSAFVFPTGKGNSYRAVEISAPSNTTDAYKAEFFYDSISVN
                     TSNIDTTLGYVTRNRYWKLNRTNGSSNVYVSLYWGGSYAVHDTAKTYISGWNGTKWLN
                     LGKVIYGNNNSGKVTSSGTVTSYSEFALAISSTNPMLCASTPQIIFPKEYFTFDVVSG
                     SGYDYNQSSDPLTPSIASTSSSFALPSITSANIGTLSGTTQNYLKNESSAASSLTCSN
                     PVDLTKGVICVEMLVKLDANFVNQRGLRFFNFKDNSGDNAFYGGIGYPYLYLNAKVID
                     NISDPNNPIQIEKRLQIPLDGVDRKSIDYYNDQGWHHFVFKINTHNNENPPSTAPGRL
                     EIWVDGELNDFYLDDADLNNSLSILQPEFRLLDAGIDCFLGKMDNIAIYDDDVCPEQL
                     YNNYKTCFPLLTNLEPVANYCYDNTNCDLSLITSIPTTPLTGAMNPNDFPENYTINTA
                     PLSDGDLIDYINSTCSCNSSDPNWFVELDDFLQNSYDAQFTGVALPLDQLKSYPLPRY
                     FPGHSFRQNVNFMGVNYLGYSGYPRSGGSYSISANAPTYSTDIQKELSENWNYSFVVC
                     ENSSTYPNTFTQAWINAANSYPADSPVKFAITTNRNHLPNSKVKKQDFVPDEDYYLMS
                     YDWNPTPPTLSPNQFLDSDGNIVSSPKILKPLNDPTYLSNFLPDGFDQNVIFSSIFGS
                     LTKNVSYVIENNEYTPQFSYNSSGIPAVANTINGDYTLSQGCSTCATPDDWKSYLGEF
                     KKTLDNDYRDQFFGGTNFPQLADAVYLNYAVDGFDKYNKNYRFEYTNMRETQSQVMYG
                     GSSQYLSTPDFYPRRAANWLTTVSADHGIDWVNKCRTNEIDNFSDYRYAPFVAAGWEQ
                     QEEFNIRPAQWLSLVKAAGIMGVDFYNTGYFNLNAFWFANPSGYVWQAVIPSYAQAVF
                     SHAEDYFTNGTLSKGNVLLEVTDASFGYGYRFAGSLPNDLIMIRKINNEDKILITGSL
                     QPLSNISGNVPDDRDIVINDLPFSSTTSAKINFRRQGSTYIFDNTNPGDPVFYQLDKW
                     HEYKHPERWSKDFDFEAEVFDNKNVNVVIKTVDATSNSSITNGDFTNYTSYITFAATP
                     DNTGVQYNFTPRADGVNNDYDIWVRARSRSGTRTGFTITTTSVGNSNFTQDNKVSCVS
                     SGSWEWYKLDDCTGSAMTLSSLTDDQQYVLTLLPMSDDLEIDRILLDVDNNAGFTAAS
                     ACGYDKDIPDNTSSTTVSISNNDFVNIQGTYIVNSNLSLTGCIINVEAGGEIDVQSGG
                     TFSCDNCHISACGDMWNGINNTGGTVTLTSSTVNDALAAVKSVSGNAVDVTGCVFDHN
                     YTAIWLENGSYSASDIYGNTFENTGGQINKAPHAGDDPLHHIYLNNVSAVTVGDASQA
                     VNTFHNALNGIYAEASNLNVYNNIFNNIGGNNYQNRAIVPPVPPISIPNAAINARGVK
                     AGYKTLNVGGASTGQSNTFNQCEIGVSGYMQLNADIRNNAFAECYTGIALSSIGGSGN
                     TVNIEDNTIDKYNKGISLSNVWMNNSTVIKDNDLNLGAGYDAAHYGAMGIEVSNFITR
                     QVKLTIENNHIENAQNGIQVSNVGYTISPVGYAPYADVKDNTVLFAIPVGLTANHVGL
                     WLENMAYGTVENNTVHWTETPSGAGSELMQGLRLSNSVHCGITGNAIENMESGIMVFG
                     NCSQTNLYCNKFESCFNSTYFSNNNSSNQLTDQGLKVETMGTYDPTLSEGWKNHWVSP
                     VSDKAAGVLLAPNIFNWMYKPSVSFDYDPGNFTGYFTTLSADNETGSCTPAFTTDEGL
                     RDQAFGSLVYDS"
BASE COUNT         4444 a         2563 c         2850 g         4158 t
ORIGIN      
        1 gcccatatac aataggaaaa gctatcacca attcgcaggt ttatatatta gataaaaaca
       61 gacgacctgt tcctgttggt attgcaggag aactttatat ggccggtgat ggtcttgctc
      121 gcggttatct ctataaacct gaaatgactg aagagcgttt tgtggctaat ccttttgttc
      181 ctggacaaag gatgtataga accggtgacc ttgggaagtt tatgtctgat ggaaacattg
      241 aatttttagg ccgaatagat aatcaggtaa aaattcgcgg atttagaatt gaacttggtg
      301 aaatagaatc aatgttgcga ttgcgaaaag agataaagga acttgtagta atggcacgtg
      361 aagatcaacc aggaaataaa attttagttg catacctttc tactaatgca ggaaagcaga
      421 ttgatgtcaa tgaactacgg gaatatctga gaacagaact tcccgaatac atgatacctt
      481 cggtatttat gattcttgaa tatattccgt tgacacctaa cggaaaaatt gacagaagag
      541 ctttgcctgt tcccgaagtg gaagttaaag cagaaagtgc aaactttgaa gaacctcaca
      601 accctttaga aagtttgctt gtaggtattt ggagtgaagt gctgggaatt gataaaattg
      661 gtattcacga taattttttc gagttgggag gacactcgct tcttggaatt agaatgatgg
      721 gagccataga gcagaagaca ggagttaaat tggattatcc aacattattc cgtgcttcaa
      781 ccatttcgca attaactaag gtgatgaatg cagaagagtt gaacattgac tggccaatag
      841 tggttccatt gcagcctaat ggaaaagaaa gaccgttgtt ctttgttcac atgcacaatg
      901 gaaacattca gcgatggaga gtcttattaa aacatttgga taaggaccaa cctatttatg
      961 ctattcaacc ccgcggactt gatgaaaaat atgactatca ttattctgtt gaggcaatgg
     1021 cttctttcta tattgatgag attaaaaaga ttcaacctgt aggtccatac cgaattgccg
     1081 gattatgttt tggtggaaca actgcatttg aaatggctcg tcagctaaaa gcaaaaggtg
     1141 aaactgccga acttgtgttt atgattaaca attatgcgcc accatccaat ccaatgcaat
     1201 ataaaatccg cgaaacatgg gatgaattta aaggcatgac tttggaagat aaacttgaat
     1261 ttgcacttga gaaaacaaag aatgcaggaa agaaagtttt aagcaaactg actaaggttt
     1321 ttgattccgg cagcactaca gcctctgaag ttaaagtaaa tactaagcct gacatacgtt
     1381 gggtaaattc agtagccttg ctcaattatc agcccgaagg tgaatattct gaaaaagtag
     1441 ttttaatcaa aactggtgaa gaagttgcca aacattatga cgagacattg ggatggaaac
     1501 gacttataag tggcgagata gaaatgcatc agattaccgg aagcgataac gatacaatca
     1561 ttacccataa agaatattat acgcagttag cgcagtattt aaatcaggcc ttaaaccaaa
     1621 ttaaataacc gctgagatgg atttgcagtt aactccggaa caggtttttt gcaatgaatt
     1681 attaattcag gacaatgatt gcttagtttt tattttctgt tcagactcat ttatagcagt
     1741 agaaagtgaa ttgaaaaaac ttttaagcaa tgatgaaaca gaaagacttc ttaaatttaa
     1801 gtttgaaaaa gacagacaga cctattgcat tgcacatgga ttgaaaagag ttttacttgc
     1861 tgcattgctc aatacaaaag aacaagtaga gattagtaca cacagcaatg ggaaaccatt
     1921 tattaaccat aaaaaacata atatgccgct acattttagt ttgagtcatt cagaaaaaat
     1981 gatagcactg gcatttcaga aaaatagccc gttgggtgta gatatagaaa aaattcaacc
     2041 ggcagataaa tatggaaatg tgattcagca ttattttact tcggatgagc aaaagctaat
     2101 ttcccggtca gacaatgata tcttttttaa aatctggact cagaaggaag ccattggaaa
     2161 agccacaggt cagggtatag ccaatttacc tgaaattgtc attccggatg actatgagtt
     2221 aagtactgca tctttaggcg gatatatttt aagtacatgt tttttcagag gtaatgagat
     2281 tagttttaag acaattcatg cacaggatgt tttaaatcag attaatatac tatctttgcg
     2341 cccataatta acattataat caaccggata attttttgaa aaacactgat aattccggtt
     2401 cttccatctt caaatgaaca aaacttcttt ccatgagctt tcgttaagtc atgaaatgct
     2461 ccaggctatt gacgaaatgg gtttcactga ggcttcgccc atacaagcac aagccatacc
     2521 tgtattgatg cagggtcagg acgtaatagg gcaggcgcaa acaggtaccg gaaaaacagc
     2581 agcttttggt attcctttac tcgaaatgct cgaccctaaa gttaaggcta cctctgcatt
     2641 ggtcatgtgc ccaacacgcg agttagccgt acaggttgca caagaaatta aaaaattagc
     2701 caagtataaa aaaggtattg ctgtgcttgc cgtttatggt ggcgagcaaa ttcaaaagca
     2761 gataaatgca ttaaaacgcc ctgtacatgt aattgttggt acaccgggca gaattatgga
     2821 tcatttggac agaagaacta taaacctttc taaaattcag atggtagttc ttgatgaggc
     2881 tgatgaaatg ttgaatatgg gtttcagaga agatatggaa gcaattttgc aacatgcccc
     2941 tgacgaaaga cagacggtgt tgttttctgc aactatgcca aaacctattc ttgaaatcac
     3001 caaacgtttt caaaccaatc ctaaattagt taaggtcacc aagcaggaac taactgtatc
     3061 atctattgaa cagatttatt acgatattcc atcgtcacag aaagctgatg taatcacaca
     3121 attagtagag ttacacaatt taaaactgat gcttgtattc tgtaatacca aacgtaaaac
     3181 tgatgaggta accgagcagt ttcagcaatt gggctataag gcagaggcta ttcatggcga
     3241 cttgcgacaa aatcaaagga ataatgtgtt ggcacgcttc aaaaaaggtg atgtgaatat
     3301 gcttgtggca acagatgtag ctgcacgagg catagatgtt gagaatgttg atgctgtatt
     3361 taatttcgat cttccactcg atgaagaaaa ttatgtgcat cgtatcggca gaacaggccg
     3421 tgccggtcgt tcgggtaaag ctttcagttt tgtttccggg cgaaatgata tggctaaaat
     3481 ccgtgagatt gaagattatt caaaagtgag gatagaaaga catcagctgc catcaggaaa
     3541 ggagcgtata gagttggcta aaattaaatt ggcagcaaga ttgaaagttc ttattgagga
     3601 aggcgatatg gaacaatatg acaacatgtt tgcaactttc aagcaggaag gattaacatt
     3661 acatcaactt gcagcggcat tgcttaaaat gcattttgca cctatgttgc aacaaagtca
     3721 aaataagtca aacagtaaat catcgagtag agaagaacga tttggcagag aggaaagatc
     3781 cggcagaggt aatcgtgata gcggtggtgg caaaggcaaa cgcggtaaac acagtggcga
     3841 agccgatatg attcgtttat ttttaagcgt tggcaaaaaa gataaagtaa gtaaaggtga
     3901 tattgttggc gcagtttccg gtttaagcaa tatgagtagt agcaatattg gtgttattga
     3961 tatttacgat aagttttctt tcgttgaagt caatggagag tttttaaatg atgtgctttc
     4021 caacgttaac ggcaataaaa taaaaggccg taaagtaaat gttgaaattg ctaaagacta
     4081 atttaatcta ctgtgtatgc cagtagaaaa tctttttctc ttctgtaaca aattttaatc
     4141 atttcctctt gactgtgttg tatcagcaca gtaggcatac ctaaatttaa caattcaaaa
     4201 ggttgaatgt gtatgcagtt tttaaagtct attcctgata cattgaagta tttaatcgca
     4261 gcttctttaa tgcaccaaca tgtcagtgca tgtagatttt ttttctgttg agaaagaaaa
     4321 gcaatttcat tagtatttag aaaatagtca attccggctt caatatttct atctgtcatt
     4381 tctatgtcaa taccggcact ttttgattgt gtaatcatta atgcagtata atttccggag
     4441 tgtgaaaagg agattgcatt gttaccatca atgtatggtt tacctgtttc ctgatagtgt
     4501 aattgaagtg atggattttt tgttacttgt ttaagcaact ttgtgttaag ctctttttga
     4561 aatgtttttc tgctttcttt ttctgaggga taatttgctt gcaccacata caattgataa
     4621 ttgctgttga tttcattcag aataaaagaa aacactattt gcatggtgct aagataacag
     4681 ttgtttgttg ttgattttta attttgcaac atgacatcgc ctgaagtttt tcagtttggt
     4741 attccggtta cttctgtaat tgattcaatc aatattacag ggcaggaaat agctttttat
     4801 agaatcacca acatagatgc attgtatagt aagttaagca gccttaagaa tgatgatgac
     4861 aatgttattg atgaacgcat tccttattgg gcagaattgt ggccttcggc aattgcactt
     4921 gctgagtatt tgtgtactcg gaaactgaat aatgatatgc taattcatga aattggctgt
     4981 ggtctggcac tgccttcatt ggtttgcggc aaaatgggtt acagcgtgat gatgagtgat
     5041 tatgttgacg ctccattgca atttgcatct gccaactgga gactcaatgt aaaatccgaa
     5101 ccgacaacaa ttttattgga ttggcgcaaa ataccagatg atattgaaaa atgtgatttg
     5161 ctcttagctg ccgatgttgc ttacgaaagc agaatgtttc ctgccattgt aaatgcattt
     5221 aaacgattgg ttaaatcagg aggcagaatt gttttcactg agccaaatag aaaatttgct
     5281 tccggctttt ttgagttgat tagacatgaa tttaccaaca ctaccataca aaacagagag
     5341 gtaattcaaa acaatcacaa acatcagatt aaaattgtag aaattgaagt ggagtagaag
     5401 agagcgcaaa aatattatcg gatggtttga taaaacctga ccaattgctc accaattata
     5461 ttgttgttgt attggcttct tgccagcaat tgtgcctcct ttgcaatact gtttaatttt
     5521 tccggattgt tcagcaaggc taaaatagtg tcagcaaact gttgaggcgt atcagcaata
     5581 agaatatttt ttttgtctgt gtaattaatt ccttctgcac ctatgcttgt tgttacaatt
     5641 acttttcctg ctgcaagtcc ttctaatatt ttaatcctaa tgccaccacc actcaatagc
     5701 ggaacaacca taatctgttt atcctgcata aactttttcg agtctttgat ttcaccttca
     5761 ataatcaggt tttctgattt cagattgaaa tattttggcg acatattctt tccggctaag
     5821 tgtagcttaa tcttttttga ttgttggttt atcaatggcc agatatcttt caataaccag
     5881 tcaacggctt caatattagg gagccagtcc attgatccaa gatgaaataa tgaaagacta
     5941 tcagcatcca tctgttctat gttcttgtat ttggcagtat tcattcctgt tggcgccact
     6001 tcaataggaa tattacagcc atctttcaga aaaagatttt tatctggctg cgatatagca
     6061 atgattgcgt caaatttatt tatggcaata ttttcataat gtttgagtct gtcggataaa
     6121 aaataaagat aagttttttt taatggattt tttgttgctt tagcaagtct tttccagata
     6181 agccattcca cattatgtgc ccgcaatatg cacttggctt tgctgaatgt acgcactgtt
     6241 tgtaggtaag ggctaagaaa taatccttca aacaatatga aatcaaatgt ttccgattgc
     6301 agcagttctt tcagcttttg gtgaaacgct tccgagtcaa aacggacaat gttatatgac
     6361 tcgtttggtt tgaacagatt ggcaaaagca tcccatgcgt ttacagaggc atcaagaaaa
     6421 actgtttcca atttatattt ttcaagcaat gcggcatcaa tggaatttat gtccgtaaaa
     6481 tgttttttgg tgttgaacga aagcattgtt acctctgcgc ccgatgccgt gagtgattcg
     6541 gcaaggttgt acatggcaat ggtggcgcca tcataaggcg gcaacggaat acgcaaacac
     6601 agttgaagga ttttcaaatt aaagaaggat tctaaactta tgtgcaaact taaaaaaatt
     6661 aacaccctta aaccttgatg ataaattaat ttggatatga aaaggaaaac tcattaattt
     6721 tacaaatctt taaaaataac attactacca aactaaaatc ccaactatca tgcaaccaac
     6781 caaattaaaa agcggcacag tatgcgggaa cttgcttgct gcgctactgt tgttttcaac
     6841 aagcagcata gcccaaccat ctgatgctta cgtaagcatg aaggctactg ctgttgcagg
     6901 tactgtacac ggagtgtatt acattgaact ggccgacacc aacagtgtga atcaaattga
     6961 aatcaaactc ggcaccgact ggacatctgc cgacatggta aatgcagtgt ttacttatga
     7021 tacacccggc agcctgccgc agggttacag ttacactcgc aacggaaaca tcattgccat
     7081 tgacatgggc aattttcccg aacagtcttc ttactacgga aacgtaagag taaaaaatgg
     7141 tgcaacatgg agcaataatt acacctttgt ttcaaactga ttgtattata aaatcaaatt
     7201 atgtttaatg tttaacattt aaaaatcaaa taccatgaaa agaatatatt cactcctcat
     7261 ctatattgtg tttataaata catttcctgc actggcaacc acttatacat ggacaggaaa
     7321 cacaagcact gcatggaaca ctacgtctaa ctggtcgcca tcaggaacac ctacaacagg
     7381 cgattttgta cacatttaca gtgctacata caatccgcaa cttacaggca acctaacctt
     7441 aaacggaatt tatatacaca gcggaacgct taatttgaat ggcgatacct tgttgcttaa
     7501 ctccggtaca agcactttta gcggtggcac cataagcaat ggcttgttgc aactgcgcgg
     7561 aacagaaatt acatttaaca gcaccacttt taatgctccc attgatgcag tagttgaccg
     7621 tattaacctg aacggatgca cgtttaacaa ttactgttat tttgaaacca tttccaatgt
     7681 acccggtacg ggaagcggtg gagcagtgtt taacgacagt gtttacctgt atcataatta
     7741 cacagggtcg ggaatttggg tgatgaacaa caacaccgga caccgcttta acggtaaagt
     7801 aaccatacat aacaaaggaa caaaagaaat cacttttaca ggtgcgggca ttaatgtatt
     7861 taatggtgat atagttttaa taaacaccgg agcgggagga attatgttag gtaatgatac
     7921 gctcacagca gggcatacca tcagcattgg aagcggaggt ttttcggcag gcgattttaa
     7981 tgctactaat ttttatcagg atggcaacac tgctataagc ctcaccttta ccggaacagc
     8041 attgccaagc ataacaggag caacgtttgg aggtaatttc agcattactt ccaatgtgct
     8101 gttatttaag aactgtacgt ttagcggcac ggcatcattt accgccacag gtagcggcag
     8161 cagcagttgg tatggcggca atacatttta tggcaatgcc acttttacca acaacgctac
     8221 ggcaggcaac cttcgcattg atgccgacac tgccaacacc tactatgccg atgccacctt
     8281 taacggctcg cgcgagctaa gggtgtgcta tggtggtgag gtaagctcct tttacggaaa
     8341 cataaccaca gcaaacaaca ccacctttaa tgcaggcaca ggcacactgt gtcttgccgg
     8401 cagcggaaca caggcgttga gctttcacag taatacgctt gtaaaaaatc tgatgctgaa
     8461 caagagcggt ggcagtgcag cattgtcaac ggcattggta gtaagcggaa cggtaacgtt
     8521 tacaaaagga acgctcacca ccacaagcag caatttgctt acgataaaca acggtggcac
     8581 agtgagtgga gcaagcgaca gcagctttat tgacggacct gttaagaaaa taggcaacag
     8641 cgcatttgtg tttccgacag gaaaaggaaa cagttacaga gctgtggaaa taagcgcacc
     8701 ttcaaatacg actgatgctt ataaagcaga atttttttat gattccataa gtgttaatac
     8761 atcaaacata gatactacat tgggatatgt tacaagaaac cgatactgga aactgaacag
     8821 aaccaatgga tcatcaaatg tgtatgtcag tctttattgg ggaggtagtt atgcggtgca
     8881 tgacactgcg aaaacatata tttcagggtg gaatggaact aaatggctga atcttggtaa
     8941 agtaatttat ggaaataata attcgggaaa agttacatca tcaggcactg ttacatcata
     9001 ttcagaattt gcattggcta tctcttctac taatcctatg ctttgtgctt caactccaca
     9061 aattattttt ccaaaagaat actttacgtt tgatgttgta agtggtagcg gttatgatta
     9121 taatcaatct tcagatccat taaccccatc aatcgcatca acatccagca gtttcgctct
     9181 tcctagtatt accagtgcca atattggaac actttctgga acaacccaaa actatttaaa
     9241 aaatgaatcc tctgctgcat cttctttaac ttgctcaaac cctgttgatt taacaaaggg
     9301 ggtgatatgt gttgaaatgc tggttaaact tgatgcaaat tttgtaaatc aaagagggtt
     9361 acgttttttt aattttaaag ataattcagg cgataatgct ttttatgggg gtattggcta
     9421 tccatatctt tacctaaatg ctaaagttat tgataatatc agcgatccta ataatcccat
     9481 tcaaattgaa aagagattac agattccatt agatggtgtt gacaggaaaa gcatagatta
     9541 ttataatgac caaggatggc accatttcgt ttttaaaata aatactcata ataatgaaaa
     9601 tccaccttca actgcaccgg gaagattaga aatttgggtt gatggcgaat taaatgattt
     9661 ttatttggat gatgctgacc taaataattc attgagtatt ttacaaccag agttccggct
     9721 tttggatgca ggaatagact gttttttagg taaaatggat aacattgcta tttatgatga
     9781 tgatgtttgc cctgaacagc tttataataa ttataaaacg tgttttcctc ttctaaccaa
     9841 tttagagcct gttgctaact attgttatga taatacaaat tgcgacctta gtttaataac
     9901 ttccataccc accacacctc tcacaggtgc aatgaatccg aatgattttc cggaaaatta
     9961 tactataaac acagctccat tatcagatgg tgatttaatt gattacataa actcaacatg
    10021 tagttgtaat tcatctgatc ccaattggtt cgttgaattg gacgattttt tacaaaactc
    10081 ttatgatgcg caatttacag gagtagctct tccgttagat caattaaaat catatcctct
    10141 gccacggtat tttcccgggc attcattcag gcaaaatgtt aattttatgg gggttaatta
    10201 tttaggatat agtggatacc cccgaagcgg aggttcttat tcaatatctg ctaacgcacc
    10261 tacctacagt acagatattc aaaaagaact ttctgaaaac tggaattact cctttgtggt
    10321 ttgtgaaaat tcttcaactt atccaaatac ttttacacaa gcatggataa atgctgcaaa
    10381 tagttacccg gcagacagcc ctgtaaaatt tgcaattact actaatagaa atcatttacc
    10441 caactctaag gtaaaaaagc aggacttcgt gccggatgaa gattattatt taatgagcta
    10501 tgattggaat ccaactcctc ctactctgtc tccaaatcaa ttcttagatt ctgatggaaa
    10561 tattgtttct tctcctaaga tacttaaacc cctaaatgat ccaacttatc ttagtaattt
    10621 tttacctgat ggatttgatc aaaatgttat attttccagc atattcggta gtttaacaaa
    10681 aaacgtaagt tacgttattg aaaataatga atatactcct caattcagtt ataattccag
    10741 tggaattccg gcagtggcta atactattaa tggagattat accttatctc agggatgcag
    10801 tacttgtgca acacctgatg attggaagtc ttatcttgga gaatttaaga aaactcttga
    10861 caacgattac cgtgatcagt tttttggagg aactaatttt ccacaattag ctgatgctgt
    10921 ttatttgaat tatgccgttg atggttttga taagtataat aaaaactaca gatttgaata
    10981 cacaaacatg agagaaacgc aatcgcaggt aatgtatggc ggttcttctc aatatttatc
    11041 tacccctgat ttttatccgc gcagagctgc caactggtta actaccgtta gtgctgacca
    11101 cggaattgac tgggtaaaca aatgcaggac aaatgagata gacaatttta gcgactatcg
    11161 ctatgcgcct tttgttgctg ccggatggga gcagcaggaa gaatttaata tacgacctgc
    11221 acaatggctg tcattggtta aagcagccgg tataatgggt gttgactttt ataacaccgg
    11281 ttattttaat ctgaatgctt tttggtttgc caatccgtca ggatatgttt ggcaggcagt
    11341 aataccaagt tacgctcagg ctgtattcag ccatgcggaa gattatttta ctaacggaac
    11401 attatctaaa ggaaatgtac ttttagaagt tactgatgct tcatttggat atgggtaccg
    11461 gtttgcagga agccttccga acgatcttat tatgattcgt aaaataaata atgaggacaa
    11521 aatactgatt accggatcgc ttcagccgct tagcaatatt tcaggaaatg tgcctgatga
    11581 tagagacatt gtgattaatg atttgccgtt ttcttcaacc acatccgcca aaattaattt
    11641 caggcggcaa ggctccacct acatttttga caataccaat cctggtgatc ctgtatttta
    11701 tcaacttgac aaatggcatg agtataaaca tcctgaacga tggagcaagg actttgattt
    11761 tgaagcggaa gtgtttgata ataaaaatgt caatgtggtt attaaaaccg ttgatgcgac
    11821 ttctaattct tctattacca atggcgactt taccaattac accagttaca taacctttgc
    11881 cgcaacacct gacaatacag gcgtgcaata taactttaca ccaagggctg acggggtaaa
    11941 caatgattat gatatatggg tgcgggcacg cagcagatca gggacaagaa caggctttac
    12001 aattacaacc acatcagtag gcaacagcaa tttcacacaa gataataaag tatcgtgcgt
    12061 aagttccggt tcatgggagt ggtataagct ggatgattgt acaggctcgg ctatgacatt
    12121 aagcagcctt accgatgacc agcaatatgt gctcacgctg ctgcccatga gcgatgattt
    12181 ggaaatagac agaattttat tggatgttga caacaatgca ggatttactg ccgcctcggc
    12241 atgtggttat gataaggata ttcctgataa cacaagcagc actacagttt ccatttcaaa
    12301 taacgatttt gtgaatatac aaggcacata cattgttaat agcaaccttt ctctcaccgg
    12361 atgtatcata aacgttgaag caggtggcga aattgatgtg cagtcaggcg gcacattcag
    12421 ttgtgacaac tgccatattt cagcttgtgg cgatatgtgg aatggcatta acaacacagg
    12481 aggtaccgta acgcttacca gctcaaccgt taatgatgcc cttgctgccg ttaaatccgt
    12541 ttcgggcaat gctgtggatg ttacaggctg cgtgtttgac cataattaca cggctatatg
    12601 gcttgaaaac ggcagttaca gcgcaagcga tatttacgga aacacttttg aaaacactgg
    12661 cgggcaaatt aataaagcgc ctcatgcagg cgatgatcct ctgcatcata tttatttgaa
    12721 taacgtatct gccgttacgg ttggcgatgc ttcacaggct gttaatacct ttcataatgc
    12781 ccttaacggc atatatgccg aggcttccaa tctgaatgtg tataataata tctttaacaa
    12841 cattggcggt aataattacc aaaacagggc tattgttcct cctgttcccc ccatttcaat
    12901 tcctaatgca gccattaatg caagaggtgt taaagccggt tacaaaacct taaacgtggg
    12961 aggtgcatca accgggcaga gcaatacttt taaccaatgc gaaataggtg tttccggtta
    13021 tatgcagttg aatgccgaca tcaggaacaa tgcatttgcc gaatgttata ccggcattgc
    13081 attaagctcc attggcggaa gcggcaacac cgtaaatata gaagacaata caatagacaa
    13141 gtacaacaaa ggcatttcct tgtcaaatgt gtggatgaat aactccactg taataaaaga
    13201 taatgatttg aacctgggtg caggctatga tgccgcgcat tacggagcta tgggcattga
    13261 agtaagtaat tttattacgc ggcaggtaaa gctcacaata gaaaacaacc acatagaaaa
    13321 tgcgcaaaac ggcatacagg tgagcaatgt gggttacacc ataagcccgg tgggttatgc
    13381 gccttatgct gatgtaaaag ataacacggt gctgtttgcc atacctgtcg gattaacggc
    13441 aaaccatgta ggcttatggc ttgaaaacat ggcttacggt acggttgaaa acaatactgt
    13501 gcactggact gaaacaccct ccggtgcggg cagtgagctt atgcagggct tgcgcctgag
    13561 caacagcgtg cattgcggca ttacaggcaa tgccattgaa aacatggagt cgggcattat
    13621 ggtgtttggt aattgcagcc aaaccaattt gtattgcaac aagtttgaat catgttttaa
    13681 ctccacgtat ttcagtaaca acaattcttc caatcagcta actgatcagg gtctgaaagt
    13741 ggaaactatg ggaacttatg acccaaccct gagcgaaggc tggaaaaacc actgggtaag
    13801 ccccgttagc gataaagcgg caggagtttt attagctcct aatatattta actggatgta
    13861 taaaccttca gtctcttttg attatgaccc gggtaatttc acaggctatt ttaccacatt
    13921 atctgccgat aatgaaaccg gaagctgcac gccagcattt accaccgatg agggcttgcg
    13981 cgaccaggca tttggttctc ttgtttatga ttctg
//
LOCUS       DAOOOL010000068        13954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_384_length_13954_cov_66.588891, whole genome shotgun sequence.
ACCESSION   DAOOOL010000068 DAOOOL010000000
VERSION     DAOOOL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_13954_cov_66.588891"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKU82_06890"
     CDS             <1..622
                     /locus_tag="PKU82_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HOZ82636.1"
                     /translation="DANGHFCFSYSPFDKQVVFNASMKGSRLLAQVYSVTKDESLKKA
                     AADSVAFVMKHQRADGAWIYSTSDAGTWIDNYHTGYVLDCLHEYVLHCDDKTYDENLK
                     AGKKFYINHFFLEGRIPKFYNNNVFPVDCTAAAQSLLTLTRFNEQTLAFKVAEYMLEK
                     MQHQKGYFYFRKYKNYTEKTSFMRWSNAWMFAGLTAVLQSLSANRQ"
     gene            802..1851
                     /gene="lpxD"
                     /locus_tag="PKU82_06895"
     CDS             802..1851
                     /gene="lpxD"
                     /locus_tag="PKU82_06895"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOZ82637.1"
                     /translation="MKFTAKQIADLLKGTVEGNHDAEVNALSKIEEGKPGTLSFLSNP
                     KYTPYIYDTKASVVIVNKDFVAENAISATLIRVEDAYKSFASLLEIYNQIVNDKKGVE
                     SPSFIDPSATVGDQGYIGAFAYIGKNAVIGKNVKIYPHCYIGDHVIIGDNTTLFPGVR
                     VYSFCQIGMHCNIHAGVIIGADGFGFAPNENNNYKKVPQIGNVVIHDHVEIGAGTTID
                     RATLGSTVIHKGVKLDNLIQIAHNVEIGENTVIAAQTGVAGSTKIGKNCMIGGQVGII
                     GHLSIADGVKIAAQSGIGTSITTPNEIVQGSPAFNISDYKRTYVVFKKLPQLERKINE
                     LEKKTGSLNPAENKS"
     gene            1848..3242
                     /locus_tag="PKU82_06900"
     CDS             1848..3242
                     /locus_tag="PKU82_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOZ82638.1"
                     /translation="MSVKQVTLKEEITVEGPGLHTGVNARLTLKPAPENHGYKFKRTD
                     VNGQPIIDADVDNVVDTSRGTTLSANGVKVSTTEHVLAALVGMEVDNVLIEIDGPEVP
                     ILDGSAFPFVNAIQKAGLREQEATRSYFVITENLTYEEPARNVEMLAVPSPDYRITVM
                     VDYNSPVLGTQHAQMHKITEFKDEISKCRTFVFLHELEMLLKHDLIKGGDLNNAIVIV
                     DREIKQDKLDELATLFKKPKVEIKHKGILNNIELHFQNEPARHKLLDIVGDFALLGVH
                     LKGHILAARPGHAANVAFAKQFKEIIRKDKNRLKAPIIDANKPALFDISRIESFLPHR
                     FPFLMIDKITELTETGVVGVKNVTANEWFFQGHFPGNPVMPGVLLIEAMAQTGGILVL
                     NTVPDPGNYWTYFLKIESARFKNMVKPGDTVVFKLDLISPIRRGLCHMGGQAFVGDKV
                     VMEAEMMAQIVKKS"
     gene            3239..4036
                     /gene="lpxA"
                     /locus_tag="PKU82_06905"
     CDS             3239..4036
                     /gene="lpxA"
                     /locus_tag="PKU82_06905"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOZ82639.1"
                     /translation="MISPLAVVSPKAKIGNNVTIEPFTMVYDDVVIGDNTWIASNVTI
                     YDGARIGSNCRIFPGAVISAIPQDLKYKGEQTTAEIGDNTTIREFVTMNKGTTDKMKT
                     TLGSNCLVMAYAHIAHDCQLGNHIIMANNVTLAGHVLIDDWAILEGLVAVQQFVHIGA
                     HSFIAGGSLVRKNVPPFTKAAREPLSYIGINNIGLRRRGFSNEDVNHIQDIYRILFVQ
                     NSNVKKALAEIEKEIPESALRSQIVEFIHNSPKGLMRGFTPSDSDDE"
     gene            4059..4685
                     /locus_tag="PKU82_06910"
     CDS             4059..4685
                     /locus_tag="PKU82_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ82640.1"
                     /translation="MFTVNLTEAGKKFKQHWIFRKCTAAFSSGDVIGITGKNGSGKST
                     LLQVISGYVSPNEGKIEWHEHDKKIEADSVYRYFSIASPYLMLIEDFTLEEMIRYYFR
                     FKNLRPAFNISSIITESGLEAHRNKLLSEFSSGMKQRTKLLLAIASDTPLLLLDEPCT
                     NLDKQNIAWYQQFLRTHSDGRLIFICSNHIDDELFLCNKWLSVEDFKI"
     gene            4907..5083
                     /locus_tag="PKU82_06915"
     CDS             4907..5083
                     /locus_tag="PKU82_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82641.1"
                     /translation="MMNNDPIITYDHTKEENSFRSHYFKKSFFGRKNTMLLFFAEELL
                     ILGFMAYLVYLVFV"
     gene            complement(5324..7126)
                     /locus_tag="PKU82_06920"
     CDS             complement(5324..7126)
                     /locus_tag="PKU82_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82642.1"
                     /translation="MKKSIICLVLLFHLTVLLFVACRKNDSDDVIPNVQQTSEQIRTT
                     ISGYVLNEMGQALSGVTVSTNGYIATTNDKGIFLLSKVIVDKNRCVISFSKSGFTKRN
                     YVFRPTDSSGQYVKLMLLNNVPNKTVSGVNGGTVSLPNGATIEFQPNSFTTANGTAYT
                     GTVNIYLKHLSPDDTDFSMMMPGGDMLAKDTTGKIVSLYSFGMMMAELTSTNNLPLQL
                     AAGKPATISMPIAPSQILKAPATIPLWYFDETTSLWIEEGGAAKVGSAYTGQVKHFSF
                     WNCDFPGERATIKGRIIDCNGNPMPNVVVTLDNWFDMLTDNNGVYQSWVPANISYVLQ
                     VLLSHNSGVFSSAPVNITPIAPGQTYTVPDISLPCPSSITGTLQNCAGVNVAGYVAVL
                     NLNANLLNLIYTGSGQFSINVPSQIPIVIYVSSLNNFEHYDTLNAISNMGTIINLGSI
                     QLCTYANDTTNLITINGAGFNNQTFIFTNALFMTSDLPSDTSIYSHELELGGILQPYG
                     SCYYYGISLKDTLAGTQPIINSTQVDFQLQTPQVDYAINLLPGGIIELHSNALIPGDY
                     FEGTYSGQADIINLNTVTSTQGTVIGKFRVRKQF"
     gene            complement(7280..7657)
                     /locus_tag="PKU82_06925"
     CDS             complement(7280..7657)
                     /locus_tag="PKU82_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82643.1"
                     /translation="MHTIEQLEEMLAEHKEWQGKINFYRAEIDRMKTDLASAVMADRN
                     QYNMPHVEHYQNQFILQRDILDIMRHDFKQHENKIEAHSSNPVTNLAAAHQSEREKLG
                     SFERYFKELRNEFQAFIHKEAFS"
     gene            8194..8640
                     /locus_tag="PKU82_06930"
     CDS             8194..8640
                     /locus_tag="PKU82_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ82644.1"
                     /translation="MKRIAEIALMSMCVLGVCKSTFVNAQSNIAERKYRVIAYKQGAN
                     EITSMSNETTVTPNMAIYVPNSFTPNGDGINETFGAYSEGVKDFTMQVYDRWGAMIFE
                     SNDISKRWDGMYKGQIAQQGSYVYKIQAKAINGRILTKKGTVNLIM"
     gene            8837..9574
                     /locus_tag="PKU82_06935"
     CDS             8837..9574
                     /locus_tag="PKU82_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82645.1"
                     /translation="MKHIAKLLWLTFFIPLLSSTASAQDTEEETSRWQIGLTAGINIP
                     LSDFADKLDENFKSGYAMLGATSRLRTSYHPNSKLYIAADYVWFYNPSDAKTLLNSLV
                     ETNPSGPRYKTTTYPWKVNSLLLGLGTTLNFNEQTDATLRGMLGFANGKYSKRIYQSF
                     DGVKTLQVTENASSVIDIALNLGFEVKYSFHKNVGVMFAGDYFRTEFNYDDVYLIYSE
                     NGEKRSTGAYSQPVDVFQFSAGIFTKF"
     gene            9887..>13954
                     /locus_tag="PKU82_06940"
     CDS             9887..>13954
                     /locus_tag="PKU82_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82646.1"
                     /translation="MFLLAVPTCLVAQNYQPKSYYTFEGSSPMKDSMGNFNLDPNYYQ
                     SSYQILTNTSGGVGKYMKLDSSSTIIRGGVLAIDSAITFEFIFKPGMNFGTTVFAKRM
                     DGALELRMTYPAINFMTEVKAANGSPIYDDFEIDLNGIGRASYSYYVDDNWHHIVFKY
                     NAKAGIKEVWVDGQLPAGFSKTIAGGSFSQSGNRDYMLNSQSDYYKLQGAIDEIAVYH
                     NSLGGNSIYAHYLNFMAGKHYSFANPTVVAPSPSPVTAGININDFAPGHPSPSLTPIE
                     QLKNFPTPRYKPGHTLFPNNPFVGYYYLSGYQTYETVYSKAVANSVTLQTDFAKNFNY
                     TIMAAENTGAYSDYSNSTTFSHAWINLANSYPQYKVSALSYWPQLRPTDAGYKSNSPY
                     ATCGCLPDKYYLKNSSGQYLNPNGGTGSTKYLSPAGAPDSLVKDGLTQKFYMQQLLAK
                     LSRPIDYILDNGEVVPFLYYTNVMQSDPSVVSDKNSSGLDWFTYEGRRAATKINAYRD
                     QFMGLSGLSNTKFAYYQLDGQPTWNLKWSEVRTVNTAMNGSHYATGDIYMRWPSNWRY
                     WSGAWKGWQWVVESRREQLLAGDKYFSPAVSPGWDNDETQNVRPAQWLGFLKAVSMTG
                     AEFFTTGYFVTSQPYQNPKNYIWQLVIPPYAQAITSRYEDLFRNGYLMSGDVPNSTTS
                     PTYMAYSFNTGDPGKLVVVRKHNSQNKYAITGSSQPMSNMAGAAPDEAVAQINLDGSR
                     IKFKIRRQGSTYIYDNTNTSAPIFYQLDGWHENTYPYYWSKDFKFEAELNDNSNSNVY
                     LKTTRPAGAAAGDFTDFTTAVGFNSVSDLTYNFQPRSTTDYTYYVWVRARSKNSQTTG
                     MTISLDGSNSKQIGCITDANWQWYKYDVNSGAVISFTSVTSLADHILKITPTNTNLEI
                     DQIHLSTSSNAIYTSPAPCSGVAVPTISASGPVAFCAGGNVNLIASNGLSYLWSNGST
                     DPYINVTSAGSYVVTVTTAAGTATSAPVTVTVYAQPNATITANGSTSFCVGGSVTLTA
                     SSGSTYLWSSGATTQSITAIGGGNYNVIVTNSNGCSAMSASTAVTVKPVPTATISPSG
                     PTTFAAGGSVTLTSSSAGTGGSYKWSPGGMTTQAVTVSTTGNYVVTVTNNSGCSATSS
                     PLSVTVTSTAPTPKITANGPTTFCQGGSVVLTCSAANSYLWSNGATTQSITVTTAGSY
                     TCTATTAGGSATAAAVNVIVTTPTQATITASGSTSFCSGGSVTLTASTGTSYMWSTGA
                     TSNAITTSSPGTYTVTITNNGCQSTTNKVVTVYALPTATITANGSTNLQQGGSVQLTV
                     SSAGSGGSYLWSTGATTQSITVSTAGNYTVKVTNSN"
BASE COUNT         4556 a         2590 c         2588 g         4220 t
ORIGIN      
        1 cgatgccaat ggtcattttt gtttttctta ttcaccattc gataaacagg tagtttttaa
       61 tgcaagcatg aaaggctctc gtttgttggc tcaggtttat tcagtaacaa aagatgaatc
      121 cctaaaaaaa gcagcagcag attctgttgc ctttgtcatg aaacatcaac gtgcagatgg
      181 tgcatggatt tactcaacca gcgatgcagg aacatggatt gacaactatc acaccggtta
      241 tgttttagat tgtttgcatg aatatgtttt acactgtgac gacaagactt atgatgaaaa
      301 cctaaaagca ggaaaaaaat tttacatcaa ccactttttt cttgaaggtc gtatcccgaa
      361 attctacaac aacaatgttt ttccggttga ctgtacagcg gcagcacaat cacttctgac
      421 attaacacga tttaacgaac agacccttgc atttaaagtt gctgaataca tgctggaaaa
      481 aatgcagcat caaaaaggtt atttttattt cagaaaatat aaaaattata cagaaaagac
      541 atcttttatg cgttggagta atgcctggat gtttgccgga ctgactgctg ttttacaaag
      601 cctgagcgcc aataggcaat aaggcatttg tccatagcag ttttcagcat taacaaatcc
      661 atggcttggt tatacccttg ccaaccactg ttttagcatt attttttagt tgaatagtat
      721 ttattggttg agtttgtata tcacagtctc atttgtacat ttgcacacat tgcaccaagt
      781 ccggaattta ttgagtaaca aatgaagttt accgccaaac aaatagcaga cctcctcaaa
      841 ggcactgtag agggaaatca cgatgccgaa gtaaatgctt tgtctaaaat cgaagaaggc
      901 aagcccggaa ctttatcctt tttatcaaac ccaaaataca caccatacat ttacgacacc
      961 aaggcatctg ttgtcatagt aaataaagat tttgtggctg aaaatgcaat tagtgcaaca
     1021 ctcatcagag ttgaagatgc ctataaaagc ttcgcctcgc tgcttgaaat atacaatcag
     1081 attgttaacg ataaaaaagg tgttgagtcg ccttctttta ttgacccttc tgcaacagtc
     1141 ggagatcaag gttacattgg tgcttttgca tatattggca agaatgcagt tattggtaaa
     1201 aacgtaaaaa tttatccgca ttgctatatt ggtgatcatg taattatcgg tgacaacaca
     1261 actttatttc ccggtgttag agtttattct ttttgtcaga taggcatgca ttgcaatatc
     1321 catgcaggtg taattattgg tgcagatggt tttggatttg ctcctaacga aaacaacaac
     1381 tataaaaaag ttccacagat aggtaatgtc gttattcacg accatgtaga aattggtgca
     1441 ggaacaacta ttgatcgtgc tacattaggc tcaactgtca tacacaaagg tgttaagtta
     1501 gacaacctga tacaaattgc acataatgta gaaattggcg aaaacacagt tattgctgca
     1561 caaacaggag tggcaggttc aaccaaaata ggaaagaact gtatgattgg cggacaggta
     1621 ggtatcatag gacacttatc tattgctgat ggcgtaaaaa ttgctgcaca atccggcatc
     1681 ggaaccagca tcacaacacc taacgaaatt gttcaaggtt ctcctgcatt taacataagc
     1741 gactacaaac gcacctacgt tgtgtttaaa aagttaccac aattagaaag aaaaattaac
     1801 gagttagaaa aaaagacagg tagtctaaac cctgctgaaa ataaatcatg agtgtaaaac
     1861 aagttacttt aaaagaagaa ataactgtag aaggtccggg gttgcacacc ggtgtaaatg
     1921 ccagacttac attgaagcct gcacctgaaa atcatggata caagtttaaa agaactgatg
     1981 taaacggcca accaattatt gatgccgatg tggacaatgt tgtggatacg tcacgcggaa
     2041 caactttaag tgcaaacggt gtaaaggtga gtacaacaga acatgtttta gctgcattgg
     2101 taggcatgga agtagataat gtgcttattg aaattgatgg ccctgaagta cctattcttg
     2161 acggcagtgc gtttccattt gtaaatgcca ttcaaaaggc cggcttgcgt gagcaagaag
     2221 caacacgtag ttattttgtt attacagaaa acctcaccta tgaagagcct gcccgcaatg
     2281 ttgaaatgct tgctgtgcca agccccgact atcgtattac ggtaatggtt gactacaact
     2341 cacctgtgtt gggaacacaa catgcacaaa tgcataaaat tactgagttt aaagatgaga
     2401 tttcaaaatg cagaacattt gtattcctgc atgaacttga aatgctgctt aaacatgatt
     2461 taataaaagg tggcgacctg aataatgcaa ttgtaattgt tgaccgcgaa ataaaacagg
     2521 ataagttaga cgagttggca accttattca agaaaccaaa agttgagata aaacataaag
     2581 gcattctaaa taatattgaa ctgcattttc agaatgagcc tgcacggcat aaactgttag
     2641 atattgttgg tgactttgca ttacttggtg tgcatctgaa aggacatatt cttgctgcgc
     2701 gtccggggca tgctgccaac gttgctttcg caaaacagtt taaagaaatt atcagaaaag
     2761 ataaaaaccg tttaaaggca cccatcattg atgcaaataa acctgcacta ttcgacatta
     2821 gcagaattga atctttcctg ccacatcgtt ttccgttttt gatgattgac aaaatcactg
     2881 aactgactga aacaggtgtt gtaggtgtaa aaaatgtaac tgccaacgaa tggtttttcc
     2941 agggccactt tccgggtaat cctgtaatgc ccggtgtgtt gctaattgaa gctatggcac
     3001 aaacaggtgg tattcttgta ctcaatactg ttcctgatcc tggaaattac tggacatact
     3061 ttttaaaaat agaatcagca cgttttaaga atatggttaa gccgggcgat actgttgtct
     3121 ttaagcttga tttaatttca cctatcagac gtggcctttg ccacatgggc ggacaggcat
     3181 ttgtaggcga caaagttgtt atggaagcag aaatgatggc tcaaattgta aaaaaatcat
     3241 gatttcacct cttgcagtag taagccctaa agcaaaaatc ggtaacaatg taaccattga
     3301 accttttacc atggtttacg atgatgttgt tatcggtgac aacacatgga tagcttcaaa
     3361 cgtaacgatt tatgatggtg caagaatcgg cagcaactgt cgcatttttc ctggtgcagt
     3421 aatttctgcc attcctcagg atttaaaata taaaggcgaa cagacaacag cagaaattgg
     3481 tgacaacact accattcgtg agtttgttac catgaataaa ggaacaacag acaaaatgaa
     3541 aaccacattg ggcagtaact gcttagtgat ggcttatgca cacattgcac acgattgtca
     3601 gttgggaaat catattatca tggcaaacaa cgttacgctt gcaggacatg tattaattga
     3661 tgactgggca atattagaag gcttggttgc agtgcaacag tttgtacata ttggtgcaca
     3721 tagttttatt gcaggaggat cgttagtgcg gaaaaatgtt ccgccattta ctaaggcggc
     3781 acgcgaacca ctttcatata ttggcataaa caatatcgga ctcagacgca gaggtttttc
     3841 taatgaagat gtaaatcaca ttcaggatat ctatcgcata ttgtttgttc agaacagtaa
     3901 cgttaaaaaa gcacttgccg aaattgaaaa agaaattcct gaaagtgcat tgcgaagcca
     3961 gattgttgaa tttattcata attcgcctaa aggtttgatg cgtggattta ctccatcaga
     4021 cagcgatgat gaataattga acaacaaaaa atcacatcgt gtttactgtt aacttaactg
     4081 aagccggaaa aaaattcaaa caacactgga ttttcagaaa atgtactgct gctttttctt
     4141 ctggtgatgt tattggcata acaggcaaaa acggctctgg caaatcaact ttacttcagg
     4201 ttatttcggg ctatgtatca cctaatgaag gaaaaattga gtggcatgaa catgataaaa
     4261 aaatagaggc cgactctgtc tatcgttatt tttcaatagc ctcaccctac ttaatgctta
     4321 ttgaagattt tactttagag gaaatgatcc gctattattt ccgttttaaa aacctacggc
     4381 cagcatttaa tatttcttct atcattactg aatccggcct ggaagcacat agaaataaat
     4441 tgttgagtga attttcttct ggcatgaaac agcgaaccaa gttactgctt gccattgcat
     4501 cagacacgcc acttttactt ttagatgaac cttgcaccaa cctcgacaaa caaaacattg
     4561 catggtatca gcaattttta agaacacatt cagatggcag attgattttt atctgttcaa
     4621 accacattga tgacgaattg tttttgtgca ataagtggct ttccgttgaa gatttcaaaa
     4681 tctgaactgc aaaacatagc ttgtatcgtt tattctttca tgcgttctaa agattgaatt
     4741 ttttttcttt aaaacatctt tctctctgaa aaatctgttc gtcacaaaaa aagggtatca
     4801 aaaaaaccaa ttacttttct gaatttctta catcttaaac agaaattcat tttttatccc
     4861 attccggcaa agtgcagata taaattgcct gcaaattctt atgactatga tgaacaacga
     4921 tccaattatc acctatgatc acaccaagga agaaaactca ttccgctcgc attactttaa
     4981 gaaatctttt tttggacgaa aaaatacaat gctgctgttt tttgctgaag aattgctgat
     5041 tcttggcttt atggcctact tagtctattt ggtgtttgtg taaaaatttg ttttggaaat
     5101 taagacttct ggaacaattg aaaatctgtt atgctatgta gtaaatttgt ttttactgat
     5161 aaaaaataac aacgaatcag gcaaagcttt ttagaaaaaa aagtaaaaag ttttagaata
     5221 taaatatata aatagtagcc gttactcaaa aaagtgtaac ggctacattg ttttatttct
     5281 ttcattataa caatttatct cgaattttta tttcttatac gaatcaaaat tgttttcgta
     5341 ctctaaactt tcctataacc gttccctgtg tacttgtgac tgtattcaaa tttattatat
     5401 cggcttgacc ggaataggtt ccttcgaagt aatcacctgg aattaatgcg ttcgaatgta
     5461 attctattat tccgccaggt aataaattta ttgcataatc aacttgagga gtttgtaatt
     5521 gaaaatcaac ttgagttgaa ttgattatag gttgtgtacc tgcaagagtg tcttttaaac
     5581 tgataccata ataataacaa ctgccataag gttggagaat tccccccaac tcaagttcat
     5641 gagaatatat tgaggtatct gaaggtaaat cagaagtcat aaataaagca ttagtaaaaa
     5701 taaaagtctg attattaaat cctgcaccat ttattgtaat taaatttgta gtgtcattag
     5761 cataagtaca aagttgaatt gaacctaaat ttattattgt ccccatatta ctgattgcat
     5821 ttaatgtatc gtaatgttca aagttgttca gtgaagagac atatataact attggtattt
     5881 gtgatggcac attaattgaa aattgtccac tgcctgtata aattaaattt aataaattag
     5941 catttaaatt taatacggca acatatcccg caacatttac tcctgcgcaa ttctgcaatg
     6001 ttcctgtaat tgaagaagga caaggcaatg aaatatcagg tactgtataa gtctggccag
     6061 gagcaattgg tgtgatgttg acaggtgcac ttgaaaagac tcctgagtta tgacttaata
     6121 aaacttgcaa cacgtaactt atatttgcag gtacccaact ttgatagaca ccattattat
     6181 ctgttaacat atcaaaccaa ttatctaatg ttaccacaac atttggcatg ggattaccgt
     6241 tacaatcaat tatccttcct ttaatagtag ctctttcacc tggaaaatcg cagttccaaa
     6301 aactgaaatg tttaacttgc ccggtatagg cactccctac ctttgctgca cctccttctt
     6361 caatccacaa ggatgttgtt tcatcaaaat accataatgg aatagtagca ggggccttta
     6421 atatttgtga aggagcaata ggcattgaga ttgttgccgg tttacctgca gcaagttgta
     6481 aaggcaaatt attggttgat gtaagttctg ccatcatcat accaaatgaa tacaaactta
     6541 caatcttacc agttgtatct tttgcaagca tatctccacc aggcatcatc atactaaagt
     6601 ccgtatcatc aggtgaaaga tgtttcagat atatattaac tgttcctgta taagcagttc
     6661 cattagcagt ggtaaaagaa tttggttgaa attctattgt tgcaccatta ggtagtgaaa
     6721 cagttcctcc attaactcct gaaactgttt tgttaggcac gttatttaac aacatcaatt
     6781 taacgtactg tccgcttgaa tctgttggac gaaaaacata gtttcgttta gtaaaacctg
     6841 atttagaaaa actaataaca caacggtttt tgtcaacaat aactttactt agtaaaaaaa
     6901 ttcctttatc atttgtagtt gctatataac catttgtaga aacagttacc ccactaagtg
     6961 cttgccccat ttcatttagt acatatccac taatagtggt gcgaatttgt tctgacgttt
     7021 gttgaacatt aggaataaca tcatccgaat catttttgcg acaggctaca aatagtaaaa
     7081 ccgttaaatg aaacaataac actaaacaga taattgattt tttcatgact atcattttaa
     7141 tctaagtaaa atgtaacata gcttttattg atgaacaact ccattcaata aatataacac
     7201 tttcactatt tcctgattct tttaaacaaa atcagcagga ataattactt gactacacaa
     7261 atctatactt cgttgcctct cagctaaaag cttctttatg aataaaagct tgaaactcgt
     7321 tgcgcaactc tttaaaatat ctttcgaaag aaccaagctt ttcgcgctca ctctgatgtg
     7381 cagctgcaag atttgtcacc ggatttgaag aatgtgcttc aattttattt tcatgctgtt
     7441 taaaatcatg acgcataata tctaatatgt cgcgctgcag aataaattga ttctggtaat
     7501 gctcaacatg tggcatgttg tactgatttc tatcggccat cactgctgag gcaagatcgg
     7561 ttttcatcct gtctatctct gcacgataga agttaatttt accttgccac tctttgtgtt
     7621 cggcaagcat ttcctcaagt tgttcaatgg tgtgcatgtt attaagattt agagtgaacg
     7681 atataatggc ggtttatcta tacaacacac ttctttggaa tgagaagcca caagaaagaa
     7741 aaatatcttg cctgaaaaga atagtgatga aaaataaata ctgtaactat aatttttcat
     7801 tctaaagcag actaaatttt tcctaaaagt aatacttttt atcattaaac aagcatattg
     7861 ttcaaaaact tctcaaaacg ttggcagatt catgtgtttt ttaggcttat caacagaata
     7921 agaatcttca atatcggaag gacttgtttt taatatgcta tacatggtct aattgtattt
     7981 ataatggctg ctgaaatcgt ttttctttca cataaaaagc acaagtcttt ccgattcaga
     8041 tatatttttc tcagaggaga attcacaaaa acgtgcttaa atctttaact caggatgata
     8101 aaatcttaca tatgtgattt agctgcgctt ttcaaacaat taaaccacat tcgggaaagc
     8161 aatttgcaat tcactgtaca aataataaat aatatgaaaa gaattgcaga aattgcctta
     8221 atgtctatgt gtgtgttggg tgtgtgtaaa tccacctttg taaatgcaca aagcaacatt
     8281 gcagagcgca agtacagggt cattgcctac aagcagggag ccaatgaaat caccagtatg
     8341 tctaacgaaa ctacggtgac acctaatatg gctatttatg tgcctaactc ctttactcct
     8401 aatggcgatg gtataaacga aacatttggt gcttatagcg aaggtgttaa agactttacc
     8461 atgcaggttt atgaccgctg gggagccatg atttttgaat ctaatgacat cagcaagcgc
     8521 tgggatggta tgtataaagg ccagatagcc caacagggaa gctatgtata taaaatacag
     8581 gcaaaagcta taaacggcag aatattgact aagaagggaa ccgtaaacct tattatgtaa
     8641 tatcaaagaa ggcagatcat aatgtttcac tataaaaaca aagaacattg tcaatcatac
     8701 tactaaaata tctcatcagg cacttcgaca aaaagggaat agtagtttcg caaatacata
     8761 agctgaaaac taaaattgcc ttgcttggtt tgtgattcct gtaaagttgc tactttaggg
     8821 caaactttcg ggctacatga agcatatcgc aaaattatta tggcttactt tttttattcc
     8881 tcttttatcg tcaacagcct cggcacagga tactgaagaa gaaacatcac gctggcagat
     8941 aggtctaaca gcaggcataa acattccatt gagtgatttt gccgacaagt tagatgagaa
     9001 ctttaaatcc ggctatgcca tgcttggcgc tacctcgcga ctgcgaactt cttaccaccc
     9061 aaacagcaaa ctatatattg ctgccgatta tgtgtggttc tacaaccctt ctgatgcaaa
     9121 aacattgctg aacagtcttg ttgaaacaaa cccttcggga ccacgataca agacaactac
     9181 ttatccatgg aaagtcaaca gtttgctttt aggacttggc acgacattaa attttaacga
     9241 acaaacagat gccacattaa gagggatgct tggttttgct aatggcaaat attctaagcg
     9301 tatatatcaa tcttttgatg gtgtaaaaac attacaggtt acagaaaatg cttcttctgt
     9361 tatagatata gcactcaact taggctttga agtaaaatac tcttttcata aaaacgttgg
     9421 agttatgttt gctggcgact atttccgtac agaatttaac tacgatgatg tgtatttaat
     9481 ttattccgaa aacggagaaa aaaggtcaac cggtgcttac tcacaacctg ttgatgtttt
     9541 tcaattttca gccggaattt ttaccaaatt ttaatgggcg aaagttcgct ttttttgggt
     9601 gaaagtcatt tttttgatgg cgaaggttga gttattaaca ggtgttaata acactatgcg
     9661 ctgattatca tcattttaac gtactgaagt cgaaaaggtc ttttttgggt gaaaattcga
     9721 tttatttttg gacgcgttaa ccaattcttc tattccgttt aacaacaaaa tttattaatg
     9781 cgaaaaaact tcggttaatc gcagggggct tattatgttt aatttctaat taccatttca
     9841 catgattaaa tcaaatttaa tctcaattac aaggagcatt gttgcagtgt tcttgttagc
     9901 agtaccaact tgtttggttg ctcaaaatta tcagccaaag tcgtattata ctttcgaagg
     9961 ctcctcgcca atgaaagatt cgatgggcaa ttttaatctt gatccgaact attatcagtc
    10021 aagttatcaa atacttacca atacttcagg tggtgttggc aagtatatga aattggactc
    10081 aagcagtacc atcatcagag gtggtgtact tgccattgat tctgccataa catttgagtt
    10141 tatttttaaa ccgggcatga actttggcac aacagtattt gccaagagaa tggatggcgc
    10201 acttgaattg cgcatgacct atccggcaat taacttcatg acagaagtaa aagctgctaa
    10261 tggctcacca atttatgatg attttgaaat tgatcttaac ggcattggcc gtgcatcata
    10321 cagttattat gttgatgaca actggcatca tattgtattt aagtataatg ctaaagccgg
    10381 tattaaagag gtttgggttg acggacaatt acctgcagga ttttcaaaaa ccatagcagg
    10441 tggtagtttc tcacagagtg gcaacagaga ttacatgttg aactcacaat cggattacta
    10501 taagttgcag ggtgcaattg atgagattgc agtttaccac aactcacttg gtggcaattc
    10561 tatttatgct cactatttga attttatggc gggcaaacac tattcatttg caaacccaac
    10621 ggttgttgca ccttcgccaa gtccggtaac tgcaggaatc aacatcaatg attttgcacc
    10681 cggacatcca tcgccatctc ttactccaat tgagcaattg aaaaactttc caacaccaag
    10741 atataagcca ggacatacat tatttccaaa caatccgttt gtaggctact attatctttc
    10801 aggatatcag acttatgaaa ctgtttattc aaaagctgta gcaaacagtg ttacactgca
    10861 gacagatttt gcaaaaaact ttaactacac cattatggct gccgaaaata ccggtgccta
    10921 tagtgattac tcaaactcaa ctactttttc acatgcatgg attaatctag caaacagcta
    10981 tccacaatat aaagtatctg cattatcata ttggccacaa ttgcgcccga cagatgcagg
    11041 ttataaaagc aatagtcctt atgcaacttg tggatgttta cccgataagt attatttaaa
    11101 aaatagttcc ggacaatatc tgaatcctaa tggaggaaca ggctctacaa aatatctgag
    11161 tcctgcaggt gcaccggact cattggtaaa agatggactt actcagaaat tttacatgca
    11221 acaattactt gcaaaattga gcagacctat agattatata ttagataatg gtgaagtcgt
    11281 tcctttttta tattacacca acgttatgca atctgatcca agtgttgttt cagataaaaa
    11341 ttcatcaggg cttgattggt tcacttatga aggtcgcaga gcagcaacaa aaataaatgc
    11401 ttatcgcgat cagttcatgg gcttaagcgg tttatcgaat acaaaatttg cttattatca
    11461 gttagacggt cagccgacat ggaatctgaa atggtctgaa gtacgcacag tgaatacagc
    11521 catgaatggt tcacactatg caaccggtga tatttatatg cgttggccat ctaactggcg
    11581 ctattggagt ggtgcatgga aaggatggca atgggttgtt gaatcaagaa gagagcaatt
    11641 attagcggga gacaaatatt tttcacctgc tgtttcaccg ggatgggaca atgacgaaac
    11701 acaaaacgtt cgtcctgcac aatggttagg ctttcttaaa gctgtttcaa tgacaggtgc
    11761 agaatttttc acaacaggtt atttcgtaac gtctcaacct tatcagaatc caaaaaatta
    11821 tatctggcaa ttggttattc ctccttatgc acaagcaatc acaagccgtt acgaggactt
    11881 attcagaaac ggatatctga tgtcgggcga tgtgcctaac agtacaacaa gtccaactta
    11941 catggcttac tctttcaaca ctggcgaccc cggaaaatta gttgttgtac gtaaacacaa
    12001 ttcacaaaat aaatatgcca tcacaggttc atcacaacca atgtctaata tggcaggtgc
    12061 tgcacctgat gaagctgttg cacaaattaa tttagatggc agtagaatta aatttaaaat
    12121 cagaagacaa ggtagtacat atatttatga taacaccaac acttctgcac caatattcta
    12181 tcagttagat ggatggcatg aaaatactta tccatattat tggtcaaaag attttaaatt
    12241 tgaagcagag ttaaatgaca acagcaacag taatgtatat ctgaaaacaa cccgtcctgc
    12301 aggagcagca gcaggcgact ttacagattt caccactgct gtaggtttta attctgtaag
    12361 tgatttaact tacaacttcc agccaagatc tacaacagac tatacgtatt atgtttgggt
    12421 tagagcacgt tctaaaaact cacaaactac aggaatgaca atttccttag atggtagcaa
    12481 cagcaaacaa attggttgta ttacagatgc taattggcaa tggtataaat atgatgtaaa
    12541 ctcaggcgca gtaatttcat tcacctctgt aacatcatta gcagatcaca ttttaaaaat
    12601 tacaccaacc aatactaatc ttgagattga ccagattcat ttgtcaacca gcagcaatgc
    12661 tatttataca agccctgcac cctgttcagg tgttgcagtg ccaacaatca gtgcaagcgg
    12721 acctgttgcg ttctgtgcag gaggaaatgt aaatcttatt gccagtaatg gtctctctta
    12781 tctatggagc aatggttcaa cagatccata tattaatgta accagtgcag gttcgtatgt
    12841 tgttactgtt acaactgctg caggaacagc aacatcagca cctgttacag tgacagttta
    12901 tgcacaacca aatgcaacaa tcactgcaaa tggctcaact tctttttgtg ttggcggaag
    12961 tgttactctc actgcaagca gcggctcaac atatttgtgg agcagtggtg caactacaca
    13021 gtcaattaca gctattggag gcggaaacta caatgttata gtaacaaact ctaatggttg
    13081 ttctgcaatg tctgcatcaa cagcagtaac agttaagcca gttcctactg ccacaatttc
    13141 accaagtggt ccaactactt ttgctgccgg tggaagtgtt acattaactt cttcttcagc
    13201 aggtaccggt ggaagttaca aatggtcacc gggtggtatg acaacacaag cagttacagt
    13261 tagcacaacc ggaaactatg ttgtaactgt tactaacaac agtggttgtt ctgcaacatc
    13321 atcaccatta tcagttactg taacttctac agcaccaaca ccaaaaatta cagctaacgg
    13381 accaacaact ttctgtcagg gtggaagcgt agtattaact tgcagtgcag caaattcata
    13441 tctgtggagt aatggtgcaa caacacaatc tattactgtt accacagcag gaagttatac
    13501 atgtacagca acaacagcag gaggttccgc aacagcagca gcagttaatg taatagtaac
    13561 aacacctact caggcaacta ttacagcatc aggaagtact tcgttctgta gtggaggtag
    13621 tgtaacgtta acagcaagca caggaacttc atatatgtgg agtacaggtg caacttctaa
    13681 tgcaattact acgtcatctc cgggtacata tacagtaacg attacaaaca atggttgtca
    13741 gtcaacaaca aataaagtag taactgttta tgccttgcca actgcaacaa ttacagcaaa
    13801 tggttcaaca aacttacagc aaggtggaag tgttcaactt accgtctcaa gtgccggtag
    13861 tggtggaagt tatctatgga gtacaggtgc aacaacacaa tcaatcactg tttcaacagc
    13921 aggaaattat acagttaaag taacaaacag caat
//
LOCUS       DAOOOL010000069        13767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_395_length_13767_cov_333.987092, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000069 DAOOOL010000000
VERSION     DAOOOL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13767
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_13767_cov_333.987092"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKU82_06945"
     CDS             complement(<1..286)
                     /locus_tag="PKU82_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82647.1"
                     /translation="MSIYNPDNKSDTVERIVKDFLFSRRIATDVIALLIESGKSEKEV
                     REYFVNAFKDNHVSTVQAESWYSMLLAIANNDYLKKSMKIMDGEYIRRPSA"
     gene            complement(261..596)
                     /locus_tag="PKU82_06950"
     CDS             complement(261..596)
                     /locus_tag="PKU82_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82648.1"
                     /translation="MQKKIALVISAVPEMIDALTMISRVLDEQLTADAKENPDIAEAL
                     KVYEKIIEKVVPIEFTLNCGKVIEKGDAYYIVENGSVVWQQLSHKSDLEKIGFETPTQ
                     ANEYLQSRQ"
     gene            complement(753..1043)
                     /locus_tag="PKU82_06955"
     CDS             complement(753..1043)
                     /locus_tag="PKU82_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82649.1"
                     /translation="MHEDSVYSAFMAKESEVEIELPMVQDYKLREILDAWNSYGGMRW
                     RIRYVNDYWYVYQWGVGSWIWEKLVSHIERDKFHNELYIATVDARLIKTTLF"
     gene            complement(1098..2654)
                     /locus_tag="PKU82_06960"
     CDS             complement(1098..2654)
                     /locus_tag="PKU82_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HOZ82650.1"
                     /translation="MDQKKRVYGGTANYFTDISILEPSAGKGDILDYINDKFSGKRAG
                     LRYYCIERNQDLTHILNEKDYRVIAHDFLQYSGDYYFDFIIMNPPFSNGDEHLLKAWD
                     VLRGGDIVCLLNTETLTNPNTERKQHLLKLIEKHGSFEHLGAVFADSERPTNVRVSMV
                     RLHKVEEESAFNFKFEKVTKESQFDLNEDILGNEIARKDLVGNMMLQYDELRKAYIDY
                     NKARSRVSFYSNGLMTQYCSIFKDVIDKLNQNTLRGDYNQFCDLVKKEIWRVIFGKID
                     MDKYMTHDVRQNFSKYIDKQGAMDFTKENVAQVVEMLFTNRFTILDKAVIDVFDIFTR
                     HHKENRYLIEGWKTNDKWKVNRKVILPYYVKYGEYMSQHDLKQFGDKFSISYHRASEY
                     TDIDKVMCYITGKDFKNCTTISEALKDSFQRLGHVKNEKFDNTCTSTFFNIRFFKKGT
                     IHIEFRDAKLWEQFNLMACAGKKWLPETEQREYDAKMQYREEPELTITPKMIEANLEP
                     ITAQFELNFV"
     gene            complement(2806..4035)
                     /locus_tag="PKU82_06965"
     CDS             complement(2806..4035)
                     /locus_tag="PKU82_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82651.1"
                     /translation="MKTVKDIIKKIIKDGEVIGVNGRVLTYISKENDGTYAVVMHNDL
                     VPKLRSYATEQQLIKWFCNTSETLPVRTIEQMITEVNDAGIYYINEQGVYIKYISENR
                     WNDGKHRAVVCGPGIRASGNNAPYSDEQLIKWFGTTKWRLAGLLCNELSDNRHTVTEF
                     QDYIAQIDYNDVSPIDDVRYDKIKPLTGKITEVIQSDAIIPAYKPKKVKNVVPKPEKR
                     KNYKGTPKEQLPPEVITYRKGLSAQQVYKRRINILDRLDFDKVKALFDAVIEAQDAYE
                     YDLSEEGIIAKNINKTTKESFCRYGDANHITRTIRVKYLNDKVGVALDVQAYWMQENF
                     NKDIEPDDLAAFITNYENGAEDYPLYAKIDECKQEWKNYTGINYDSKFIKHYINRKLN
                     AEFKEYSKVPAGDGDIF"
     gene            complement(4032..4646)
                     /locus_tag="PKU82_06970"
     CDS             complement(4032..4646)
                     /locus_tag="PKU82_06970"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82652.1"
                     /translation="MHLTTVQGYGFFECSSAFQKSIRRGDETNALYFAVELFNSGYDE
                     YVWKRMKVITSEDIGLAEPNAPAIIQSLYEMYNDAKAKKHEKMPQRLHYVHAIMYLCR
                     CKKSRLIDWTMIALWREHKLEKREVPDFAFDMHNAKGKQKGRGIDHFYNDGTELTNHV
                     PQDGEEEWKQKAWKLHKEHPLPDLPVKKEKGKKNDLFDNQNSEV"
     gene            complement(4735..5835)
                     /gene="glf"
                     /locus_tag="PKU82_06975"
     CDS             complement(4735..5835)
                     /gene="glf"
                     /locus_tag="PKU82_06975"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOZ82653.1"
                     /translation="MNYLEKYDYLIVGSGLFGSTFAERMHRKGKRVLIIEKRNHIGGN
                     VWTEEMNGIHVHKYGPHIFHTNSEDVWTYVNRFCKWRQFQYSPIANYNGELYSLPFNL
                     HTFYQFYGIKTPAELQSKIEQITSKYQKPSYDNVEDFALAMLGEDLYRKLIYGYTKKQ
                     WNAEPVTISKSIIARLPIRQMFDSNYFNDLHQAIPVGGYAELVTNMTKKIEVIKNTDF
                     LSAKKEFENIAEKVIYTGSIDEYYGYELGELEYRSLRFETEQLPTENYQGVAVMNYTD
                     EHTPFTRVIEHKHFERMQSKSTVITKEFPAEWKKGMERFYPVNTEMNNALLQKYKKIT
                     NKNTLFGGRLGSYQYYNMDQIIASALKLSNSL"
     gene            complement(5813..6454)
                     /locus_tag="PKU82_06980"
     CDS             complement(5813..6454)
                     /locus_tag="PKU82_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOZ82654.1"
                     /translation="MITHDYKLIYIHIPKCAGRSITDMFNQRFDHCTAQYYQNEYPRQ
                     FKQYDVFTIVRNPYDRYVSMWNYIKNHRRHQKEDIYNQYFDDDRYDFKHWLLKNIFAY
                     KGGYPATSAEGGRGFDDKIGSAFWFSSQYSRLSNWNKSLIVPNVFHFEKMDEVYNFLS
                     ERMEQVAAIPHLNQSKRKEWTYYYDDEIIEHLHKFQPFLTDIEKLGYELLREV"
     gene            complement(6458..6778)
                     /locus_tag="PKU82_06985"
     CDS             complement(6458..6778)
                     /locus_tag="PKU82_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82655.1"
                     /translation="MYGQAMGWLAGSKIKMRNDIKCNNDIYLSLMNAYKHRMIWQDER
                     FTFDFYGYGGNKGGLASVRTEEVEKNDFLLLKEMFGDAVATKKGTWDKKPKKPYELVI
                     KLPY"
     gene            complement(6782..7204)
                     /locus_tag="PKU82_06990"
     CDS             complement(6782..7204)
                     /locus_tag="PKU82_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82656.1"
                     /translation="MGGEIDIKVVIPTAGRYDTITTHKVVDNCILCVPEEQEKFYRVS
                     CPDTEIVTHPPLVGLGAKRNWIYEKFGHVFMLDDDCKGMRRMYSENKEQDKVDSKTAY
                     DLIQFTGNMCHMAGLYVFTFNRYAQPKNYHGHSPIKLK"
     gene            complement(7189..7863)
                     /locus_tag="PKU82_06995"
     CDS             complement(7189..7863)
                     /locus_tag="PKU82_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82657.1"
                     /translation="MAKNNGNNKKEQTPPAEQEDLQLKKMKISDLVHDAKNANKGTDN
                     GKAMIKKSIESNGLGRSILIDKNNQIIAGNKTTSNAEEQGYDDVVVVETTGNTLVAVK
                     RMDIDLDTPEGRELALADNRTSEMNLNWDKDILKNVFSPEVLEKYSMSLEKKKVEVNT
                     QRYPIVPKFDESYHAIIIIAKSETQLSFLKTALSLETEKSYKNDSTGTGYVIDAENFK
                     NKWEAK"
     gene            complement(7975..8370)
                     /locus_tag="PKU82_07000"
     CDS             complement(7975..8370)
                     /locus_tag="PKU82_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82658.1"
                     /translation="MKKSLVQILCFLLLSVTLTSVSFAQDESPGKVSTYATYQPKITV
                     HSVEVCNEVMEYSFDCPESYSFTPLQEPVAKIFTGSMELSNADHAKAQCKADSKVGWQ
                     RLILNSSINKVLLADIANSGTGTSYHYRC"
     gene            complement(8415..8747)
                     /locus_tag="PKU82_07005"
     CDS             complement(8415..8747)
                     /locus_tag="PKU82_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82659.1"
                     /translation="MLYQLETKIKLTLEHEPGWTTSKHKECKIQLNIPSNFDANQYFD
                     KEGNLTANGAKSQTIVMLSGISANIHASHQAGVWDSAEHLRYIINELEKQFVQVANVD
                     VTEYGNKV"
     gene            complement(8749..9219)
                     /locus_tag="PKU82_07010"
     CDS             complement(8749..9219)
                     /locus_tag="PKU82_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82660.1"
                     /translation="MSRAYPIGIMPPNTEETIKKEVEIAIEYHNGNEDIFVELCDNVV
                     RSADYEEVMKNATERKAFGYMKLGQLKKVLDTGKLQETIDVIISGDKPKELSAPAGLD
                     APELSVEEMKRALENAMNNKNQFGHSKNHNKAWFDRKKKKSRNKQAKQSRKKNR"
     gene            complement(9219..9719)
                     /locus_tag="PKU82_07015"
     CDS             complement(9219..9719)
                     /locus_tag="PKU82_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82661.1"
                     /translation="MKKIVFLLSVIFVIGCSKNENMVPDNPASTLKAKRLAYLQQSVG
                     TFYSYDDTLGLSDSVKVTHLKDSLFLSVYSSEFDTSFIMTLDTIDADSIGYFTALPFS
                     FNVLKNVSFDDNITGCQVTNGLSTYYPEYIVFSFVIDGTYHSTLSPAMDNQPVTDFLF
                     TRQFSR"
     gene            complement(9828..9916)
                     /locus_tag="PKU82_07020"
     tRNA            complement(9828..9916)
                     /locus_tag="PKU82_07020"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9883..9885),aa:Pro,seq:cgg)
     gene            complement(10485..11534)
                     /locus_tag="PKU82_07025"
     CDS             complement(10485..11534)
                     /locus_tag="PKU82_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82662.1"
                     /translation="MESVNDFKLEDLLSYNWLLSKGYVISLPEKDNKFAIVFVARILY
                     RQVPRGKNMTASKNLSKVMDVTTRTLYKYIDCGFAIDKIFKDATYLKYTKERNDIFTK
                     PFICRIIDKSTKQEIVEFDVTIQTLYDSNLIVDISEADKADKPKLDMRKIIQLRNDIL
                     NEDIVELNDTNREDMERLAMHICEVFGTGCVTITEACDRYNVNYLTWIDWLRKNAYIR
                     QMYEEACRAAALINHTRQLSLVDNRLNALLNNGFVEETTFTYDKVFLPGRLEPVYREK
                     TKVVRRRDLDLSQLAMIKGMLYRSLISLPPSDPDDFSNWSTEEILNYVNSMDDTEKMD
                     KLSVIQTEILKNPPE"
     gene            complement(11687..11902)
                     /locus_tag="PKU82_07030"
     CDS             complement(11687..11902)
                     /locus_tag="PKU82_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82663.1"
                     /translation="MDLFNQAEEKKDYTIFNNMTDYEKRCLITTNYWKVRNGEQEIDV
                     PEEHEKYHALWKNEKARALLHKSKIIS"
     gene            complement(11902..12321)
                     /locus_tag="PKU82_07035"
     CDS             complement(11902..12321)
                     /locus_tag="PKU82_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82664.1"
                     /translation="MPINYKEYPDNWLTEIRPAVLARAENKCEECGVANQAIIKRWPD
                     GRVTYPNSAEWEMINNRVKNHDYSFTQAIKKYGFTRVVLTIAHLDHDKSNEKVPLDRL
                     RAWCQKCHLGYDMPIHVNNRKYGKKHVDNNYKLEFTD"
     gene            complement(12325..12582)
                     /locus_tag="PKU82_07040"
     CDS             complement(12325..12582)
                     /locus_tag="PKU82_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82665.1"
                     /translation="MDVMQEQFSDNIENIDDVFKSYGLQSETIELVFVQSLLKNIKPD
                     LQFGMCDKLAERICIDTMLHKISQFNMAMEKEHDLLIKQKQ"
     gene            complement(12573..13064)
                     /locus_tag="PKU82_07045"
     CDS             complement(12573..13064)
                     /locus_tag="PKU82_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82666.1"
                     /translation="MKHTKKTDRDYKHWTDSDIETLTLNYIDKTNSELAILLNRNADT
                     IRKQLNKLKLKRPLKSEIKKIEKKVKKVDFFSCLNSGRKILEQEKRKAEAIRKRDERM
                     KQEKKWADGFLEKEKPVFVPRNIPRKPVIVNDRTVIYVDASLNEEQVQAVVQKFIEKT
                     KWM"
     gene            complement(13064..13534)
                     /locus_tag="PKU82_07050"
     CDS             complement(13064..13534)
                     /locus_tag="PKU82_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82667.1"
                     /translation="MSKFSLSVNEENLFRSSLKIGNGLTVSCETIGKHPKDVSEHLKN
                     NEAFLNLCKSDLGVVNNAIIAINTNLLVDEKFDKFLQTKERAKVVITTIVFWEEYCLR
                     KEVTADKVVKVVHLYKGLGDCATSVGMLTSEFKEYIMADEKLAMYIYTNQIFIF"
     gene            complement(13527..>13767)
                     /locus_tag="PKU82_07055"
     CDS             complement(13527..>13767)
                     /locus_tag="PKU82_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82668.1"
                     /translation="IELDIMKLVNENVNNQSYERVLMEMMIFIVFVTIENNQVYESTF
                     KPIFELAKDIGVDVEALENTTSEDFAITIQQLLNE"
BASE COUNT         3639 a         2973 c         2244 g         4911 t
ORIGIN      
        1 ctgcagacgg cctacggata tactcaccgt ccattatctt catgcttttt ttaaggtaat
       61 cgttattagc tattgctaaa agcattgaat accacgattc tgcctgaacg gtagatacat
      121 ggttatcttt gaatgcatta acgaaatact ctcttacttc cttttcagat ttacccgatt
      181 caatcagcaa tgcaattaca tcggtagcaa tcctacggct gaataagaag tcttttacaa
      241 tacgttcaac ggtatcgctt ttattgtcgg gattgtagat actcatttgc ttgtgttgga
      301 gtttcaaatc ctatcttttc taaatctgat ttgtggctta gttgctgcca tactacacta
      361 ccattttcaa ctatgtagta tgcatcacct ttctcgataa ctttaccaca attcaaagta
      421 aattcaatcg gaactacctt ttcgataatc ttctcgtaca ctttcaatgc ctcggctata
      481 tccggatttt cttttgcatc tgcagtcagc tgctcatcca gcactctcga tatcatcgtt
      541 agtgcatcaa tcatttccgg tactgcagag ataaccaatg ctattttctt ttgcatactt
      601 ggtttactgt aaactacatc gacaatgcgt tctctagtcg tgaagtcaat caccgcatag
      661 tctttaaaat tgtcaacgca ccattctttt tcaaagatgt tacgttctgt tactaatgtg
      721 ctcataaata ttgtttattg gtgaaaaatt atttaaaata aagttgtttt tattaatcgt
      781 gcatcaactg ttgcaatgta taattcattg tggaatttat ccctctcaat atgtgatacc
      841 aacttctccc atatccagct gccaactccc cattgataaa cataccaata atcatttaca
      901 tacctgatac gccaacgcat cccaccataa ctattccatg catcaagtat ctcacgtaat
      961 ttataatcct gtaccatagg taattcaatt tcaacctcac tttcttttgc cataaatgct
     1021 gaataaacag agtcctcgtg catgaatttt ctataatttg aataagcgtc gtaaaacatt
     1081 gtttttgatt ttataagtta tacaaaattc aactcgaatt gtgccgttat tggctccaaa
     1141 tttgcctcaa tcattttcgg tgttatagtt agttcaggtt cttcacgata ctgcatttta
     1201 gcatcgtatt cacgttgctc tgtttccggt aaccacttct tcccggcaca cgccataaga
     1261 ttgaactgct cccacaactt agcatcccga aattctatgt gtattgtacc ttttttgaaa
     1321 aatcggatat taaagaacgt gcttgtgcag gtattgtcaa acttctcatt tttcacatgg
     1381 cctaaccttt ggaaactatc ttttaatgcc tccgaaatag tagtgcagtt tttaaaatct
     1441 ttccctgtaa tgtagcacat cactttatca atgtcggtgt actcgctcgc tctgtgatat
     1501 gagatactga atttatctcc aaactgtttc aggtcatgtt ggctcatgta ctcaccgtac
     1561 ttaacgtaat atggcaggat aaccttacgg tttactttcc atttgtcatt cgttttccaa
     1621 ccctcgatta gataccggtt ttctttgtgg tgccttgtaa agatgtcgaa tacatcaatt
     1681 acggccttat ctaaaatcgt aaatctgttg gtgaatagca tttctacaac ctgggccacg
     1741 ttctctttgg taaaatccat tgctccctgt ttgtcaatgt acttggagaa gttttgacgt
     1801 acatcatgcg tcatgtattt atccatatca atcttaccaa atatcacacg ccatatctct
     1861 tttttgacga gatcgcaaaa ctgattatag tcaccacgta atgtattctg attaagttta
     1921 tcaattacgt ctttgaaaat actgcaatac tgtgtcataa ggccattgga gtagaatgat
     1981 acccggctcc ttgctttatt gtagtcaatg tatgccttac gcaattcgtc gtactgaagc
     2041 atcatgttac ctaccaaatc ctttcgggca atttcgttac ccaaaatatc ttcattcagg
     2101 tcgaactgtg attcctttgt aactttctca aatttgaagt tgaatgcact ttcctcctca
     2161 actttgtgga ggcgtaccat gctcactctt acattggttg gccgttcact gtcagcaaat
     2221 accgctccca aatgctcaaa actaccatgc ttttcaatca gcttcagcag atgttgtttc
     2281 cgctctgtgt tcggattggt taacgtttcg gtattcagta agcatacgat atctcctcca
     2341 cgtaatacgt cccacgcttt cagcaggtgc tcgtcgccat tcgagaacgg agggttcata
     2401 atgataaaat cgaaatagta atctccggaa tactgcagga aatcatgcgc tattactcgg
     2461 tagtctttct cattcaggat atgcgtcaaa tcttggttgc gttcaatgca gtagtaccgc
     2521 aatccggctc tcttacctga aaacttgtcg ttaatgtagt ctaagatgtc gcccttaccg
     2581 gcagacggtt cgagtattga tatgtcggta aagtaatttg cagttccacc ataaactcgc
     2641 tttttttggt ccatataagg ctgtaacatc ttagtaatta catgctttgg agtagggtaa
     2701 aattctttgt caaacatgac ttttaattta ttggttaaaa aatttgttgt tgctacgata
     2761 aaaatacaaa agagtttgca aatatgcaaa ctctctcact tttttttaga atatatcacc
     2821 atcacctgca ggaactttag aatactcttt aaattcagcg ttcaattttc ggttaatgta
     2881 atgtttaatg aacttactgt catagttaat tcctgtgtaa tttttccatt cttgtttaca
     2941 ctcatcaatc tttgcataaa gcggataatc ttctgcacca ttctcataat tagtaatgaa
     3001 tgcagctaaa tcatccggct caatatcttt attgaaattc tcctgcatcc aatatgcctg
     3061 aacatctaat gcaactccta ccttatcatt tagatatttt actctgatag ttcgtgtaat
     3121 gtgatttgcg tcaccatatc tgcagaacga ttcctttgta gtcttattga tatttttggc
     3181 aataataccc tcctctgata aatcgtattc gtatgcatcc tgtgcctcaa tgactgcatc
     3241 aaataatgct ttcactttgt cgaaatccaa tctatcgagg atattaatac gacgtttata
     3301 aacctgttga gcgctcaatc ctttccggta tgtgataact tcaggcggta actgttcctt
     3361 tggagtgcct ttgtagttct tacgtttttc aggctttggc accacatttt taaccttttt
     3421 aggcttgtag gcaggtatta tagcatccga ttgaataacc tcggttattt tgcctgtcaa
     3481 tggcttgatt ttatcgtatc ttacatcgtc aataggactt acatcattat aatctatttg
     3541 tgcaatgtaa tcctgaaatt ctgtaactgt gtgacggtta tctgataact cattgcagag
     3601 taaacctgct aacctccact tagttgtacc gaaccattta atcagctgct catcactata
     3661 cggagcattg tttcctgatg ctcttatacc agggccacaa actaccgctc tgtgctttcc
     3721 gtcattccac ctgttctcac tgatatattt gatgtaaact ccttgctcat taatgtaata
     3781 gatacctgcg tcattcactt cagttatcat ctgttcgata gttcttacag gtaatgtttc
     3841 tgatgtattg cagaaccatt taatcaactg ttgttctgtt gcatacgatc gcaattttgg
     3901 caccaaatca ttatgcatca caactgcata cgttccgtca ttttccttac taatgtaagt
     3961 cagtacccgg ccattaactc ctataacttc accgtcttta atgatttttt taatgatgtc
     4021 ttttaccgtt ttcatacctc cgaattttgg ttatcaaata aatcattctt tttgcctttc
     4081 tcttttttta caggcaggtc gggtaatgga tgctccttat ggagtttcca tgctttctgt
     4141 ttccattctt cttcaccgtc ctgcggaacg tgatttgtca actccgtacc gtcattgtag
     4201 aaatggtcga tacctctgcc tttctgtttg ccttttgcgt tatgcatatc aaatgcaaaa
     4261 tccggaactt cacgtttctc taatttatgc tctctccata atgcaatcat ggtccaatca
     4321 attagacgtg attttttgca tctgcacaaa tacataattg cgtgtacata gtgcaaacgt
     4381 tgaggcattt tttcgtgctt tttggctttc gcatcattat acatttcgta taacgattga
     4441 atgatagcag gagcattcgg ctcggctaat ccaatgtctt cagaggtgat tactttcata
     4501 cgtttccaaa cgtactcatc gtaccctgaa ttaaagagtt ctactgcaaa gtagagtgcg
     4561 tttgtttcgt cgccacgacg tattgatttt tgaaatgcag aactgcattc aaagaaccca
     4621 tacccttgta ctgttgttaa gtgcatagtt tcaaatttta attgttgcta ccacaaattt
     4681 aaacaaaagt ttggctatat gcaaacgtta ttttattaaa atataaatac ttgattataa
     4741 actatttgat agctttaatg cagatgcaat aatttggtcc atattgtaat actgataact
     4801 acctaaacga cctccaaata atgtattttt atttgttatt tttttatatt tctgaagtaa
     4861 cgcattattc atttcagtat tgacaggata aaaacgttcc ataccttttt tccattcagc
     4921 aggaaactcc ttagtgataa cggtgctttt agattgcatc cgttcaaaat gtttatgctc
     4981 tatcactctc gtaaatggag tgtgctcatc tgtgtaattc ataacggcca cgccctggta
     5041 gttctccgtt ggcagctgct ccgtttcaaa tctcaaacta cggtattcaa gttcacccaa
     5101 ttcgtaaccg taatactcat cgatactgcc tgtgtaaatc actttctctg caatattttc
     5161 aaattctttt tttgctgaaa gaaaatcggt gttttttata acttcaattt tctttgtcat
     5221 gttcgttacc aattcagcat aaccacctac cggtattgct tggtgcaaat cgttaaaata
     5281 attactgtcg aacatctgcc gtattggtag ccgggcaatg atgcttttgg atattgttac
     5341 cggctctgca ttccattgct tttttgtgta tccataaatc agctttcggt ataagtcctc
     5401 tcccaacata gctaatgcga aatcctccac gttatcataa ctcggtttct gatacttcga
     5461 cgtgatctgc tcaatcttac tctgaagttc tgcaggagtt ttgataccat agaattgata
     5521 gaacgtgtgg aggttaaatg gtagtgaata cagttctccg ttgtaattgg caatcggtga
     5581 atactgaaac tgcctccact tgcagaaccg gttaacgtat gtccatacgt cctcggagtt
     5641 ggtgtggaat atgtgagggc cgtacttatg gacgtgaata ccattcattt cttccgtcca
     5701 tacgttgcct ccgatatggt tacgtttctc aataatgaga actcgtttgc ctttcctgtg
     5761 catccgctct gcaaacgtac ttccgaataa accggaacct acaatgaggt aatcatactt
     5821 ctctaagtag ttcatatcct aacttttcta tatccgttaa aaatggctga aacttatgta
     5881 ggtgttcgat tatttcatca tcgtaataat acgtccattc tttacgtttc gattggttaa
     5941 gatgtggtat tgctgctact tgttccatac gctctgataa gaaattataa acctcatcca
     6001 ttttctcaaa gtggaataca tttggcacaa ttaggctctt attccaatta ctcaacctgg
     6061 aatactgtga actgaaccaa aatgcactgc ctatcttatc gtcaaatcca cgtccaccct
     6121 ctgcacttgt tgcaggataa ccacctttgt acgcaaagat atttttcagt agccaatgct
     6181 taaaatcata ccggtcgtcg tcgaaatact gattgtagat gtcctccttt tggtgccgtc
     6241 tgtgattttt aatgtagttc cacattgaaa cgtacctgtc atacggattc cgtactatcg
     6301 taaacacgtc atattgcttg aattgacgag gatattcatt ctgatagtat tgtgcagtac
     6361 aatggtcgaa acgttgatta aacatatcgg taatactgcg tcctgcacac ttaggaatat
     6421 gaatataaat cagtttgtag tcgtgtgtta tcatgtctta atacggcaat ttaattacta
     6481 attcataagg ctttttaggt ttcttatccc acgttccttt tttggtagca actgcatcac
     6541 caaacatttc tttaagtaaa agaaaatcat ttttctccac ttcttcagta cggacggatg
     6601 ccaggccacc tttattgccg ccatatccat agaagtcaaa cgtgaaacgc tcgtcctgcc
     6661 atatcattct atgcttgtac gcattcatca atgacagata aatatcgtta ttgcatttga
     6721 tgtcattccg catcttaatc ttggaacctg ccaaccaacc catagcttgg ccgtacacta
     6781 atcatttcag tttaatcggt gaatgtccat gatagttttt aggttgagcg tatctgttga
     6841 acgtgaatac atacaaccct gccatgtggc acatattgcc tgtgaactga attaaatcgt
     6901 atgcagtttt gctatccact ttatcctgct ccttattttc agagtacatt ctacgcattc
     6961 ctttgcaatc atcatctaac ataaacacat ggccgaactt ttcgtatatc caattacgtt
     7021 ttgctcctaa tccaacaagt ggaggatgcg taactatttc tgtgtccggg cacgatactc
     7081 ggtagaattt ttcctgttct tcaggtacac atagtatgca gttatcaacc accttatgtg
     7141 tggtaatggt atcgtatcta cctgcagtcg gtattactac tttgatgtct atttcgcctc
     7201 ccatttgttt ttgaaatttt ctgcatctat tacataaccg gtgcctgtac tgtcattctt
     7261 gtaagacttc tcggtttcta aactcaatgc ggtttttaaa aatgataact gtgtttcaga
     7321 tttcgcaata ataatgatcg catgataact ctcatcgaat ttcggaacta tcgggtaccg
     7381 ttgagtattc acctccactt ttttcttttc gagtgacata ctgtatttct ccaatacttc
     7441 aggactgaat acgtttttca ggatatcctt atcccaattc aaattcattt cagatgtacg
     7501 gttgtcagcc aatgccaatt ccctgccctc cggagtatct aagtcaatat ccatacgctt
     7561 cactgcaacc agggtattgc cggtagtttc tacaaccacc acgtcgtcat atccctgctc
     7621 ctctgcattt gatgttgtct tgtttcctgc aatgatttga ttgttcttat ctatgagtat
     7681 tgaacggcct aaaccattgc tctcaataga tttttttatc attgccttac cgttgtcagt
     7741 acctttgttt gcattctttg catcgtgcac caaatctgaa atcttcattt tttttaactg
     7801 caaatcttcc tgctctgcag gaggagtttg ctcttttttg ttgttgccgt tattctttgc
     7861 catgttggtt ttatttctgt aaaaataatt attttataca actatacaaa ataaaaaaga
     7921 tgcaactttc gtcgcatctt ctaaatgttt ttaactttgt tttttttggc tcactcaaca
     7981 ccggtaatgg taagacgttc ctgttccact attggctatg tcggcaagta atactttatt
     8041 tattgaggag tttagaatta atctttgcca cccgacctta ctatcagctt tacattgtgc
     8101 ttttgcgtga tctgcgttac ttagttccat actgccggta aaaatctttg ccaccggctc
     8161 ctgcaatgga gtgaatgaat aggattcggg acagtcgaaa ctgtattcca taacctcatt
     8221 acaaacttct acggaatgca ccgtaatttt tggctgataa gttgcatagg tactcacctt
     8281 tccgggcgat tcatcctgtg caaaacttac acttgtgagt gttactgata acagtaagaa
     8341 acacaagatt tgtactaaag atttcttcat aataattcct gttttgagga tttaaaattg
     8401 attagaatat aaatttacac tttatttcca tattctgtta catctacgtt cgcaacctgc
     8461 acaaattgtt tttccaattc gttaatgatg taacgtaagt gctctgcact atcccaaaca
     8521 ccggcttgat gtgatgcatg aatatttgcg gagatacctg atagcattac aatagtctgt
     8581 gattttgcac cgttggcagt cagattaccc tccttatcga aatactgatt agcgtcgaaa
     8641 ttagacggta tattcagttg aattttacac tctttgtgtt ttgatgttgt ccaccctggc
     8701 tcatgctcta atgtcaactt tatttttgtt tcgagttggt ataacatatt atcggttttt
     8761 tttacgtgat tgttttgcct gtttgttccg gcttttcttt ttcttcctgt caaaccatgc
     8821 tttgttgtgg ttcttagaat ggccgaactg atttttattg ttcattgcat tttccaatgc
     8881 acgtttcatt tcctccacac ttaattcagg tgcatctaat cctgcaggag cgcttaattc
     8941 ctttggcttg tcacctgaaa taattacatc tatcgtttcc tgcagtttac ctgtatctaa
     9001 tacctttttc agttggccta acttcatgta accaaacgct ttccgctctg ttgcattttt
     9061 cattacttcc tcataatctg cagaacgcac cacgttatcg cataactcta caaaaatatc
     9121 ctcgtttccg ttatggtatt caatggcaat ctccacctcc tttttgattg tttcttcggt
     9181 atttggaggc ataataccga ttggatatgc tctgctcatt acctgctgaa ttgacgagtg
     9241 aataaaaagt cagtgaccgg ctgattatcc attgcaggac ttagtgtact gtgatacgtt
     9301 ccgtcaatca cgaaactgaa cactatgtat tcaggataat acgtgcttaa tccattcgta
     9361 acctggcacc ctgtaatatt gtcgtcaaaa ctcacattct tcagaacgtt gaacgagaaa
     9421 ggcaatgcgg taaaatatcc aatactatct gcatctattg tgtccaatgt cattatgaaa
     9481 gatgtatcga actccgaact gtaaacacta agaaataaag aatctttcag gtgtgtaact
     9541 ttcacagaat cggataaacc gagcgtatcg tcataactgt aaaatgtacc tacgctctgt
     9601 tgcagatatg caagacgttt ggctttcaat gtggaggccg gattatccgg gaccatgttt
     9661 tcgtttttac tgcaaccgat aacgaaaata acggacaaaa gaaatactat ttttttcatg
     9721 tctataaatt aaaaaagagg gtaggcagaa attccctctc gatgaaacat aaaaaccctg
     9781 aactaatacc actctaccaa tggcacgacg gaacccaaca ttccttgtgg tgaagtcagg
     9841 actcgaacct gaaaggactt acgctttaac tgccacatcg ctccgcaatg ttcgtttacc
     9901 aatttcgcac ctcaccaaat ccaatgccgt cgagaaaaat tagccggaat gtaaacggtt
     9961 aattttaagc attggaaaat aaagaactcc attttatagt ctttgttgac ttgtattaaa
    10021 tataggtaaa atcaattcag caactgtttt cacctgtgtc ggtactcacc tggtcttgcc
    10081 ggatattata aggcttcgtt atctcaccgg tagtgtgcat tgttagaata atcacaacct
    10141 gataagtttc tttcagtatg cctcgatgcc tacatactgt cagcttcatt aattttacct
    10201 ataagctaaa aagaaagtta atgtaaaaga acactacaaa ataaataacc tgcaaccaaa
    10261 atacaacgta aattgttagc atgatgtact gtgtatagca tccaaacttt ttctttccta
    10321 atatcctatc ttcaatgaac tttgcaacta acaactttga aaataatact actaatttgg
    10381 tgatactgat ttctaccatt atgttgttgc tactgcaaat ttaatactat ttttcaatta
    10441 tgcaagtttt tatttataat gttgtataaa ccttatttta ttctctattc aggaggattt
    10501 ttcaatatct ctgtctgaat tacactcaat ttatccattt tttctgtatc gtccatactg
    10561 ttgacgtaat ttaatatttc ctctgtgctc caattactga agtcgtcggg atcggacgga
    10621 ggtaaactga ttaaactacg gtataacata ccttttatca ttgcaagttg agataaatct
    10681 aaatccctgc gacggactac ctttgttttc tcccgatata ccggctctaa cctgcctggc
    10741 aggaatactt tgtcatacgt gaatgttgtt tcctccacaa atccattatt cagtaatgca
    10801 ttcagcctgt tatctaccaa cgataactgc cgggtgtggt taatgagtgc ggccgctcta
    10861 cacgcctcct cgtacatctg ccgtatgtag gcattctttc tcaaccaatc aatccatgtg
    10921 aggtaattta cattgtacct gtcacacgct tcagttattg tcacgcatcc tgtaccgaat
    10981 acctcgcaaa tatgcattgc caaccgttcc atgtcctccc tgttggtatc attcaattcc
    11041 acgatatcct cattcagtat atcattccgg agttgaatta ttttcctcat atccaatttc
    11101 ggcttgtcag ctttgtcagc ctccgaaata tccacgatta ggttactgtc gtacaaagtt
    11161 tgtatcgtta catcaaactc cactatttcc tgctttgttg acttatcgat tatcctgcat
    11221 ataaatggct ttgtgaatat gtcgttacgt tctttcgtgt acttcaggta agtagcatcc
    11281 ttgaaaatct tatctattgc aaatccgcaa tcgatatact tgtacagtgt acgagtggta
    11341 acgtccatta cctttgatag atttttagat gcagtcatgt ttttgcctct gggtacctgc
    11401 cggtacagaa tccgggccac gaatactatt gcaaacttgt tgtccttttc aggcagagaa
    11461 ataacgtacc ctttcgacaa tagccaatta tatgagagta aatcttctaa cttaaaatcg
    11521 tttacgcttt ccattttgtc tgtctttcac ccgatgaatt tcacctcttg gggtataagg
    11581 tatcgtttta gttttcagtt ctttataaat tcaaattctg tcgttcctcc actatattgt
    11641 gcaatattca acacttgtct tttttagcac aatgaaagca atgaaatcaa ctgattattt
    11701 tgctcttgtg gagtaatgct ctcgcctttt catttttcca tagtgcatga tacttttcgt
    11761 gttcctccgg aacgtcaatc tcctgttcac catttcgtac tttccaatag tttgtagtaa
    11821 tgaggcaccg tttctcataa tctgtcatat tattgaaaat cgtgtaatct ttcttttctt
    11881 cagcttggtt aaacaggtcc attaatcggt aaattctaat ttgtaattgt tatctacgtg
    11941 tttcttgcca tacttcctgt tgtttacgtg tattggcata tcgtaaccta aatggcactt
    12001 ttggcaccat gctcttagcc ggtccaatgg tactttctcg tttgacttgt cgtgatctaa
    12061 atgtgctatc gtcaatacca ctcttgtaaa tccgtatttc ttgattgcct gtgtgaaaga
    12121 gtaatcgtgg ttctttaccc tgttgttaat catttcccat tctgctgaat taggatatgt
    12181 taccctgccg tccggccaac gttttattat tgcttggttt gctactccgc actcctcgca
    12241 tttattctct gccctggcaa gaactgcagg tcttatttct gtcagccaat tatcaggata
    12301 ttctttatag tttatcggca tacgttactg tttttgcttt attagtaaat cgtgttcctt
    12361 ttccattgcc atattgaatt gactgatttt gtggagcatg gtatctatac atatccgctc
    12421 tgccaactta tcgcacatac caaactgtaa gtcaggttta atattcttca gtaaactttg
    12481 tacgaatacc aattcaatcg tttctgattg tagtccgtat gacttgaata catcgtcaat
    12541 attttcaata ttatcagaga actgttcctg cattacatcc atttagtttt ttcaatgaat
    12601 ttctgtacta ctgcctgtac ctgctcctcg ttcaatgatg catctacata aatcactgtt
    12661 ctatcattca caattaccgg ctttcgtggt atgttcctcg gtacaaatac cggtttttct
    12721 ttttctaaaa atccgtcggc ccactttttc tcctgtttca tccgctcatc acgttttctg
    12781 atagcctcgg ctttacgttt ctcctgctcc aatatctttc tgccggaatt taggcatgaa
    12841 aaaaagtcaa cctttttgac tttcttctca attttcttta tttcagactt cagaggacgt
    12901 ttcagcttca gtttatttaa ctgtttccgt attgtatctg cattcctatt taataagatt
    12961 gccaactcac tatttgtttt atcgatataa ttcaacgtca gcgtttctat gtcgctatca
    13021 gtccaatgct tgtaatcccg gtcggttttt ttagtatgtt tcattagaat atgaatattt
    13081 ggttagtata aatatacatt gctaatttct catctgccat aatatactcc ttaaattcag
    13141 aggtaagcat acctaccgac gtcgcgcaat cacctaatcc cttatacaaa tgaactactt
    13201 ttaccacttt atccgcggta acttctttcc ggaggcaata ctcctcccaa aaaactattg
    13261 tggtaatgac tactttggct ctctccttag tctgcaggaa cttgtcaaac ttctcgtcaa
    13321 ctaacaggtt ggtattaatt gcaatgatcg cattgtttac cactcccagg tcggatttac
    13381 acaaattcag aaacgcctca ttgtttttga ggtgttccga tacatctttg gggtgcttac
    13441 cgattgtttc acatgaaaca gtcaatccgt ttcctatctt caaagatgaa cgaaaaagat
    13501 tttcttcatt cactgatagg ctgaacttac tcattcaata attgctgaat tgtaatggca
    13561 aaatcttcag atgttgtatt ttccaatgcc tccacatcta cacctatatc tttggctaat
    13621 tcaaaaatcg gtttaaatgt gctttcatac acctgattgt tctcaatagt cacgaataca
    13681 ataaatatca tcatttccat taaaactcgc tcatacgact gattattgac gttctcattg
    13741 acaagtttca taatgtccaa ctctata
//
LOCUS       DAOOOL010000070        13735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_401_length_13735_cov_54.235965, whole genome shotgun sequence.
ACCESSION   DAOOOL010000070 DAOOOL010000000
VERSION     DAOOOL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_401_length_13735_cov_54.235965"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKU82_07060"
     CDS             complement(<1..427)
                     /locus_tag="PKU82_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82669.1"
                     /translation="MAERRMFTKKVTDDDNFMALSSSAQALYLHLSMSADDDGFCNQV
                     SVSMFKAHASVADLQQLLEKRYIYQFDNGVIVIKHWRMANALRKDRYTPTNFKEELAK
                     LKIKSNGAYTFSDDGCRVVANGLPDGCQVVATCLPQDSIG"
     gene            680..1291
                     /locus_tag="PKU82_07065"
     CDS             680..1291
                     /locus_tag="PKU82_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82670.1"
                     /translation="MIKDKYSRQRYEERKASNLCVLCGKPLDREGVVCTACNSKRTAY
                     GRELYKKLQAVGVCPRCGKNLLYGDEKSCVECRAKSAEVMSKIRATDVEKYNERQKAW
                     RKARYEKDKANGICTRCRKRKADPGHTTCTFCRETMRRARVKMPERTGRYEQGLCFFC
                     DNPVKPGYKVCEKHYQQNVKNATCEKANIARQKMKERNSQWTP"
     gene            1279..1572
                     /locus_tag="PKU82_07070"
     CDS             1279..1572
                     /locus_tag="PKU82_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82671.1"
                     /translation="MDSLKDFYDFYRPLQRKYDLRMFYKTNSKEAKITIRWRGKELVK
                     VAEETTEACFIRARRELEERMKKYEQQIETKEKAQRAGFYMDKIRESYAEKQQ"
     gene            1610..2092
                     /locus_tag="PKU82_07075"
     CDS             1610..2092
                     /locus_tag="PKU82_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82672.1"
                     /translation="MDLGYYVLYLHHGFGNKRIVRLERTINEYLERAQTENEMKTETL
                     AELLRVRYGIDVQKEINLIPMQQLIRIYQRNNPLTINDTRQILNDTAYSYMALACTAL
                     KLMFKLSVREIKEFIAEFRDLIDTLYKFNQFGLTLPKVAQCLADEVNYVDERYIKVID
                     "
     gene            2225..2635
                     /locus_tag="PKU82_07080"
     CDS             2225..2635
                     /locus_tag="PKU82_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82673.1"
                     /translation="MGKEYKTMTNREKFAEQILDIACGGSKIAVNKATLKPTACYKLE
                     CKDCLFNTHSYDYCGDKTEKWANSQYVEPPIDWSKVAVDTPILVRDNSFSEWGKRYFA
                     KYENGRVYAWSDGTTSWSGDRCTPWELAKLPEKE"
     gene            2638..3087
                     /locus_tag="PKU82_07085"
     CDS             2638..3087
                     /locus_tag="PKU82_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82674.1"
                     /translation="MNIDEFIERAQESARKHRYHADFFDINNPMRAACIKSAEDCEQL
                     AEWLEKFKEYQQLEEQDRLVKLPCKVGDEIFLDFAGFGKDIDKFTVKDFHLDCFEDGE
                     TILFCDYESNDKTLSGQIDVMEFGKTVFLTKSEAEAKLKELRGGENG"
     gene            3080..3442
                     /locus_tag="PKU82_07090"
     CDS             3080..3442
                     /locus_tag="PKU82_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82675.1"
                     /translation="MDKFLESVSKRDFDRRISEVVKMLEEKQLYGTISLIKDLKYYLD
                     LATKEKAHACNCQHDSNSRDNEPCCRCDSKHTNADRIRNMSDEELAEFLTTVTSDAIC
                     GSSWDYDGWIKELQSEAE"
     gene            3547..3975
                     /locus_tag="PKU82_07095"
     CDS             3547..3975
                     /locus_tag="PKU82_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HOZ82676.1"
                     /translation="MEDRYLFKAKRIDNGEWVTGHYVKGLNMYGKEVHLIFEPNTMFY
                     SSGETDGWYKVDPTTICQCTGLKDKNGKLIWENDIVRDIYGNFYAVFYQNNYYRFSWI
                     CVKSDVFLIGAKWHLWDINSLEVEVIGNIFDNKELLESEE"
     gene            3977..4276
                     /locus_tag="PKU82_07100"
     CDS             3977..4276
                     /locus_tag="PKU82_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82677.1"
                     /translation="MRIFKNVDEKLKEIGFNKICEDKHGAQYERYNTKYNYWQRVDIW
                     HKASGRHILQSYDRDLMDEKKIGNTCVGLTGYEMKLFLKKMKKLGLYSKTAGIEG"
     gene            4643..4798
                     /locus_tag="PKU82_07105"
     CDS             4643..4798
                     /locus_tag="PKU82_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82678.1"
                     /translation="MTDITTVVYTALIVFGIIGLTEVAFAWYDIHGRDKTDDEIQEQW
                     CSENIKH"
     gene            4864..4959
                     /locus_tag="PKU82_07110"
                     /pseudo
     CDS             4864..4959
                     /locus_tag="PKU82_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            5145..5606
                     /locus_tag="PKU82_07115"
     CDS             5145..5606
                     /locus_tag="PKU82_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOZ82679.1"
                     /translation="MAPSKEYFEELFRVSKNQIIWGGNYFNLPPTRCFIVWDKKNISE
                     DFSMAMCEYAWCSFNSNAKMFRHIPQGNANENRMHPTQKPVALYEWLLNRYAKPNDII
                     LDTHVGSASSLIACYNTNHKFVGFELDEYYYKVSKQRLDTEMAQMRLSDFM"
     gene            6093..6227
                     /locus_tag="PKU82_07120"
                     /pseudo
     CDS             6093..6227
                     /locus_tag="PKU82_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            6252..6452
                     /locus_tag="PKU82_07125"
     CDS             6252..6452
                     /locus_tag="PKU82_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HOZ82680.1"
                     /translation="MINKVPIWEKVTLTIDEAAEYSNIGINRINDMLNNPSCPFVLFV
                     GRGKRLVKRKEFEKYLEKTDSI"
     gene            6537..7724
                     /locus_tag="PKU82_07130"
     CDS             6537..7724
                     /locus_tag="PKU82_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOZ82681.1"
                     /translation="MGKDLRGKELGVGITQRKDGLYQGRYKDRFGKSKTIYNSKLSEL
                     RKELSKAVTDNQQFTSVRDSITLDAWFDRWMNVYKKKRVRPNTIREYTHIYKKNISPY
                     LGNHEITSIRKSDVQLLIDKASDDNYKYERQSKIKVILNDMFSRAMEDDLMIKNPAKG
                     VKLRADKEVNAFALTVEQQNEFFEACKGTFYDNMYNVAVNTGLRPGELFALTIADIHM
                     DEGYIDVNKTLVYQKYLEDKGKTFHVEPPKTKQSYRHVPINSVCKEYLTKQFELKKIV
                     STRRPKEQNEYLFVTRFNTPINSVIYSDSIRSVVRRINDTKSSDNEFPFFSGHTFRHT
                     FATRCFESGIEPKVVQSYLGHATLKMTMDLYTHVTPEKSFADIEKIVSADNKIIEYRR
                     KCV"
     gene            8241..8669
                     /locus_tag="PKU82_07135"
     CDS             8241..8669
                     /locus_tag="PKU82_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82682.1"
                     /translation="MNYTPNYGAYQYNPMASYQRYQQPEPTQGISGRVVQAVETINPN
                     EVPMDGSVAFFPKQDLTEIYAKSWNADGTIRTLTFKPVLNGKTDILSGDTEKLEFDLS
                     EKATEDIMAKLNELSEKIEQLSLGAQRKTPRTQSKESEKA"
     gene            8696..8863
                     /locus_tag="PKU82_07140"
     CDS             8696..8863
                     /locus_tag="PKU82_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82683.1"
                     /translation="MSNNRVMGNPMIKNAMSMAQSGNSKGIEQMARNLCKEKGINPDD
                     VMKQIRGNFGI"
     gene            8920..9687
                     /locus_tag="PKU82_07145"
     CDS             8920..9687
                     /locus_tag="PKU82_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82684.1"
                     /translation="MFNTGNCPSVPIVANLDGNNGNNWNDGSWLWFLIVVFAIFGGWG
                     NGFGGFGGANGGVGSEIQRGFDNQAVISKLDGISNGLCDGFYAMNNSMLTGFNGINAN
                     IMQTGYGIQQAVNADTVANMQNTNALQSQLANCCCETREAIQGVNYNMATNTCALQNT
                     MNNNTRDIIDSQQAGFRSILDYLCQDKIATLTAENNDLRRAASQDRQNALLTTTMAAQ
                     TNQIIDAVRPTPVPSFPASNLYGYAYNGCGCNTGCGC"
     gene            9849..10277
                     /locus_tag="PKU82_07150"
     CDS             9849..10277
                     /locus_tag="PKU82_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82685.1"
                     /translation="MAEFSSIATQTVAVNGNVLFTDAPTSVCNKGYISHRTGSGLINL
                     KGATNTCKAKYRVEFNGNIAVPTGGTAGAISLAIAVEGEPDLSTLAISTPTAVEAFNN
                     VSMATDVWLPCGCCQAISVKNTSTQEISVANANITVNRIG"
     gene            10315..10812
                     /locus_tag="PKU82_07155"
     CDS             10315..10812
                     /locus_tag="PKU82_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82686.1"
                     /translation="MECVKAKVDGIGIDNFEGQNLDDLKDFTEIVKNIVEFDKEYLIV
                     EAMENSKDDYRRYTEPLYHMPVNYNDMEYMRDMDKSRGKMYYSEPIAPHVSESNYDRA
                     KRHYTETKEMHKGASTEDKEHKMKALDMYIRELSGDISELLNDMTPDERNLLRTKMSN
                     LASKL"
     gene            complement(12037..12309)
                     /locus_tag="PKU82_07160"
     CDS             complement(12037..12309)
                     /locus_tag="PKU82_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82687.1"
                     /translation="MNDNELTTLDAVEREIRARYNGKYADVSGYQASERATRKAITDI
                     FRAVAESGTCNDITTLISGKEYRRTAFSNYLQHKNYISPIIKACYR"
     gene            complement(12706..12900)
                     /locus_tag="PKU82_07165"
     CDS             complement(12706..12900)
                     /locus_tag="PKU82_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82688.1"
                     /translation="MTRIEKMIKDGYPKIIKGNGGYRAYLKDMQPLGGGDYMAIYRYP
                     GGECCHSLEEIQKCFEIIEQ"
     gene            complement(13098..13358)
                     /locus_tag="PKU82_07170"
     CDS             complement(13098..13358)
                     /locus_tag="PKU82_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82689.1"
                     /translation="MRYCGRQKNGKALLLMDDEIINNALEQEKSGIKPHYAFYDYKKH
                     EKITPAGWLVWSLSDGGCGVVYRRKDGKMIITTGLQGDFCYC"
     gene            complement(13373..13648)
                     /locus_tag="PKU82_07175"
     CDS             complement(13373..13648)
                     /locus_tag="PKU82_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82690.1"
                     /translation="MTKAELLKEFDKLQKEKEMRIEGIHCNSNKRTIENAIECLKCPD
                     ELLEKYLTVVSLKYENIGRTIAENGDFKRHFFNRLYVFNTARQILAN"
BASE COUNT         4776 a         2354 c         2901 g         3704 t
ORIGIN      
        1 tacctatact atcctgtggc agacaagtgg caaccacttg gcaaccatct ggcaacccat
       61 tggcaaccac acggcaacca tcatcagaaa atgtgtatgc accgttggat tttatcttta
      121 attttgccaa ttcttcctta aaattcgttg gcgtataccg gtcttttctc aaagcgtttg
      181 ccatgcgcca atgcttaatt acaatcacac cattatcaaa ctgataaatg tatctttttt
      241 ccaatagctg ttgtaaatca gccacacttg cgtgagcttt gaacatggaa actgatacct
      301 gattgcaaaa tccgtcatca tcagcagaca tagataaatg caaatataag gcctgtgcac
      361 ttgatgataa agccatgaaa ttatcatcat cagtgacttt tttagtgaac atccttcgtt
      421 ctgccattta attaatctcc tatatccctt caagtttcgg ttgatgtatt ttaatctttt
      481 ccctcgtggt ttatattgtt ataccttttt cttaatgtgt tctgtacctt gttcattcca
      541 ttaatgccac cgacaataaa agctatctct gctctatttt ccgttgcctt tgtttccgct
      601 tccatatcgt gcagatgaaa agataggagg taaagaggtt tgtgagcaca attaaagctg
      661 gctttacccc tagcgaaaaa tgataaaaga caagtattct aggcagagat atgaagagcg
      721 aaaagccagc aacctttgcg tgctttgcgg aaaaccactt gatagagaag gtgtggtttg
      781 tacggcatgt aacagcaaac gtacagcata tggtcgagag ctttataaaa aattacaggc
      841 agttggtgtt tgccctagat gtggcaagaa cttactgtat ggtgatgaaa aaagttgtgt
      901 tgagtgtagg gcaaaatcag ccgaagtcat gtcaaagata cgtgctactg atgttgaaaa
      961 atacaatgag cgacaaaaag catggcgaaa agcgcgatac gaaaaagaca aggcgaatgg
     1021 catatgcaca cgctgtcgta aaaggaaagc agacccagga cataccactt gcacattttg
     1081 tcgggaaaca atgagaagag cacgagtcaa aatgcctgaa agaaccggca gatacgaaca
     1141 aggactatgt tttttctgcg ataatccggt aaagcccgga tataaggtct gtgaaaaaca
     1201 ctatcagcaa aacgttaaga acgcgacttg cgaaaaggca aacatagcac gacagaaaat
     1261 gaaagaaagg aattcacaat ggactccttg aaagattttt acgattttta ccgaccactg
     1321 caaagaaaat atgacttgcg aatgttctac aagacaaata gcaaggaagc aaaaataact
     1381 atccggtggc gcggtaaaga gcttgtaaaa gtcgcagaag aaactacaga agcctgtttc
     1441 attagggcga gacgagaact tgaagaaaga atgaagaaat atgagcaaca gattgaaacc
     1501 aaagaaaaag cacaaagagc cggattttac atggacaaaa tccgagagag ttacgctgaa
     1561 aaacagcaat aaccgcagaa aactcgtaag gcggtctttc acagacttta tggacttagg
     1621 ctactatgta ctgtatttac accatggatt tggtaataag cgtattgtaa ggcttgaaag
     1681 aacaattaat gagtaccttg aaagggcaca gactgaaaat gaaatgaaaa ctgaaacact
     1741 tgccgaactt ttgagagtta gatacggcat tgatgtgcag aaagagatta atttaatccc
     1801 gatgcaacag ttgattagga tttatcagag aaataatcca cttacgataa acgatacgag
     1861 acagatctta aatgacacgg catacagtta catggcttta gcatgtacag cgcttaaatt
     1921 gatgtttaaa ttgtcagtca gagaaattaa agagttcatc gcagaattta gagatttaat
     1981 cgatacgttg tataaattta atcaattcgg tctgacattg ccaaaggtgg cacaatgcct
     2041 tgctgatgaa gttaattacg ttgatgaaag gtacataaag gtgattgatt aatgacttat
     2101 gcatgggata acgacagcac tcaaaatgct cacataaagc agatgagaga cgataggcaa
     2161 aaagcctaca tggaaaaaca cagagacgat aaggcatatg aaagatttaa gcatatgccg
     2221 gattatggga aaggagtaca aaacaatgac aaatagagag aaatttgcag aacagatttt
     2281 ggatattgct tgtggtggta gcaaaatagc agttaacaaa gcaacattaa aaccgacagc
     2341 gtgctataaa ttagagtgta aagattgttt attcaatact cacagttatg actattgcgg
     2401 agataaaacg gaaaaatggg cgaatagcca atatgttgaa ccacctattg actggtcaaa
     2461 agttgcagtt gatacaccaa tactggtaag agataacagt ttttccgagt ggggtaaaag
     2521 atattttgcg aaatatgaga atgggagagt ttatgcttgg agcgatggaa caacatcatg
     2581 gagtggcgat aggtgtacac catgggaact agccaaactt ccggaaaagg agtagtaatg
     2641 aatattgatg aatttataga acgtgcgcaa gaatcagcta gaaagcatcg ttatcatgca
     2701 gatttctttg atataaataa tcctatgcgt gccgcttgca ttaaaagcgc agaagattgc
     2761 gaacagttag ctgaatggct tgaaaaattc aaagagtatc agcagttaga ggaacaggac
     2821 agacttgtta aattaccttg caaggtgggg gatgaaattt ttcttgattt tgcaggattt
     2881 ggaaaagata tagacaagtt tacagttaaa gacttccatt tagattgttt tgaagatgga
     2941 gaaactatac tgttttgtga ttatgaatca aatgataaga ctttatccgg tcaaattgat
     3001 gtaatggaat ttggtaaaac agtattcctc acaaaatcag aagccgaagc taagctgaaa
     3061 gaattgagag gtggagaaaa tggataaatt ccttgaaagt gtaagcaagc gtgactttga
     3121 tagaagaata tcggaagttg ttaaaatgct tgaggaaaaa caactctacg gaactatcag
     3181 tttgataaaa gatttgaaat attatcttga cttagctaca aaagaaaaag ctcatgcttg
     3241 taactgccag cacgacagca attcaagaga taatgagcct tgttgcagat gcgatagcaa
     3301 acacaccaat gccgacagga taaggaatat gtcagatgaa gagttggcag aatttctaac
     3361 gactgtaacg agtgatgcta tatgtggaag ctcatgggat tatgatgggt ggattaaaga
     3421 gcttcaatca gaagcagaat aggaggatac ttatgagtaa attaattttt ttcataattt
     3481 ttattggaat tgttatcgtg ggaattggta cgacagatta gggaaaatat gaaaggagtg
     3541 atgagcatgg aagatagata cttattcaaa gcgaagagga ttgataacgg agaatgggtt
     3601 acaggacatt atgtaaaagg tttaaatatg tatggcaaag aagttcatct aatatttgaa
     3661 cctaacacaa tgttttattc tagtggagag acagacggat ggtacaaagt agacccaacc
     3721 actatttgcc aatgcacagg cttgaaagat aaaaacggca agctgatttg ggagaatgac
     3781 attgttagag atatatatgg taatttttac gcagtctttt atcagaataa ctattatcgg
     3841 ttctcctgga tttgtgtcaa atcagatgta tttttaattg gtgcaaagtg gcatttgtgg
     3901 gacattaaca gtcttgaagt agaagttatc ggcaacattt ttgacaataa agaattgtta
     3961 gaaagcgagg aataatatga gaatatttaa aaacgtagac gaaaaattaa aagagattgg
     4021 attcaacaaa atctgtgaag ataagcatgg tgctcaatat gaacgctaca atacaaagta
     4081 caattattgg cagcgcgttg acatttggca taaagcttca ggccgtcata ttttacagtc
     4141 gtatgacaga gacttgatgg acgaaaagaa aattggaaac acttgtgttg gccttacagg
     4201 atatgaaatg aagctttttc ttaaaaaaat gaaaaagcta ggactttata gcaaaactgc
     4261 gggaatcgag ggatagtatg gcagaaaaga ataataaaga accaagccca tgtagcggtt
     4321 gcaaatacga aaaaagtaca aacataaaag agcttttagc tttttgcaca cattgtaaaa
     4381 gagcttattc ccacgaagag gatagggaaa ttcacgagga caggtatgag gagaatataa
     4441 catgacagag agtgaagcaa taagagaaaa aagaaaattc gcaatcgaac taaagcaatt
     4501 agtccatcaa aaatgtgttg aaatcaacca ctatgttagc ggttgcgaca gcccgtttag
     4561 ttatttgcag attgcagatg tacaggaaag tttgaggggg attgaaaaca ctttgaatat
     4621 taaggctaag gagtgatgca gaatgaccga cataacaaca gtagtataca ctgcgctgat
     4681 agtattcggt ataatcggtc tgacagaggt agcgtttgca tggtatgaca ttcacggacg
     4741 agataagacc gatgatgaga tacaagagca gtggtgtagt gaaaatatta aacattaatt
     4801 aatttatcag aaaggaatag gttgtgcgca cataaaaccg aggtttcctt ttggtaagaa
     4861 caaatgctgg attttggata ttacaacatg gattgtatgc aaggaatgaa agaatttccc
     4921 gacaaatatt ttgaccttgc gattgtagac ccaccattcg gggggggcaa tccgatgaat
     4981 gggaaaagaa aaaacgaggc agatttggag gacagttcga caaatatcat attgacagtg
     5041 agggctatcc tagctctaat tgttgcagaa caggcgaaac ttgggcgaaa aagtatcaga
     5101 ctaatgggaa tgcagaatta aagcaagata ttagacactg ggatatagca ccaagcaagg
     5161 agtattttga agaattattt agggttagta aaaatcagat tatatggggc ggtaattatt
     5221 ttaatctgcc accaacaaga tgctttattg tgtgggataa aaagaatatt tcagaagatt
     5281 tctctatggc gatgtgtgag tatgcttggt gttcatttaa tagtaatgca aaaatgttta
     5341 gacatattcc acaaggaaat gctaatgaaa acagaatgca cccaacacaa aagcctgtag
     5401 cactatatga atggctatta aacagatatg caaagcctaa cgacattata cttgatactc
     5461 atgtaggtag tgcgagcagt ctaatagctt gctacaacac taaccataaa tttgttgggt
     5521 ttgagcttga cgaatactat tacaaggtat caaagcagag gctagatacc gaaatggcac
     5581 aaatgagatt aagtgatttt atgtagaggt gaaacaatga aacactacaa accaattaaa
     5641 tgtgtagtct gcagcaaggt atttacaccg accgcagcca accaaaatac gtgttgcgaa
     5701 gcacatagac agcagagagc tacggacagt atatggcaga gcagtataaa aaagaggtga
     5761 tgataaaatg aatgaatgtt gtggaaattg caaatatcat caatacgagg atatatcgca
     5821 aggttgggta tgctgtaacc aagatagcga atatgtagct gattggacag attataccga
     5881 tagttgcgag gaatgggaga gcagagatga aatgtagaac tataggcgat atagagctaa
     5941 ttaaaagaca atgtgtatac gaggacaaca agccgtgcaa cagctcatgc cgatactcaa
     6001 atacttgtat acacagtgca agcaaaaccg aagaatagga gataaggtct atgaagtttt
     6061 caaagctgac tagaccggaa cttgaagaaa ttatgaaaaa tgccaatttc accgatgaag
     6121 aagcggaagt ttttgagttg ctagttgctg ataaaagcct tgaagaggta tcgcagagac
     6181 tattaatctc gaaaacaacc acttcccgga gagtggcaga cattaaagaa aagatagaaa
     6241 ggagtcaggc gatgattaac aaagtgccaa tatgggaaaa ggtaacgctg acgattgatg
     6301 aagctgcgga atacagtaac atcggaatta acagaatcaa tgatatgctt aataatccct
     6361 catgcccttt tgtgcttttt gtcggaagag gcaagcgatt agttaagcgc aaggagtttg
     6421 aaaaatacct cgaaaagaca gatagtatat agatatattg aattatgagc cattatgtag
     6481 taatatagat attatcatat aatggctttt gattttgaaa ggagccataa atcagtatgg
     6541 gaaaggattt gagaggaaaa gagctgggag tcggaataac ccagcgcaag gacggactct
     6601 atcagggcag atataaagat aggttcggca agagtaagac aatttacaac agcaagttgt
     6661 cagaactgcg aaaagaactt agtaaagcag tgaccgacaa tcaacaattc acaagtgtta
     6721 gggacagcat tacccttgat gcgtggtttg acaggtggat gaatgtatac aagaaaaaga
     6781 gagtgcgccc caataccatt agggagtaca cgcatatata taagaagaac atttcaccat
     6841 acttaggaaa ccatgaaata acatctattc gcaagtcaga tgtgcagtta cttatcgaca
     6901 aagcttctga cgataactat aagtatgaga ggcagagcaa aatcaaggtt attttaaatg
     6961 acatgttcag tagagccatg gaagatgacc tgatgattaa aaatccggcg aaaggtgtaa
     7021 agctgagagc agacaaagaa gttaatgcgt ttgcattgac agtagagcaa cagaacgagt
     7081 tttttgaagc gtgcaaaggc acattttacg acaacatgta taatgtggca gttaatacag
     7141 gcttgcgccc aggagaactg tttgcgctca cgattgcaga tatacatatg gacgaggggt
     7201 atattgatgt taataagaca cttgtgtatc agaaatacct tgaagataaa ggcaagactt
     7261 ttcatgttga gccaccaaaa accaagcaga gttacagaca cgtaccaatt aacagtgtgt
     7321 gcaaggaata tctgacgaaa caatttgagc ttaaaaagat agtttcgaca cgcaggccta
     7381 aagaacagaa cgaatatttg tttgttacaa ggtttaacac accaattaat tcggttatat
     7441 acagcgactc tatacgttca gttgtaagac ggataaatga tacaaagagc agtgacaatg
     7501 aatttccatt ttttagcggt cacacattca gacatacgtt tgcgacaaga tgttttgagt
     7561 cagggataga gccgaaagtc gttcaatcat atttgggtca tgcaacactg aaaatgacaa
     7621 tggacttgta tacacatgtt acacctgaaa aatcgtttgc tgacattgaa aaaatcgtta
     7681 gcgccgacaa caaaatcata gaatatagaa gaaaatgtgt gtagtaagtg tgtagtagta
     7741 cacacaatca attcacagaa tgttgaaaaa tcaacgctcg tagggcattt ttgtactaaa
     7801 actagtaaaa ttattacgta tatcaaggag tgccatacga tttcgtaaat aatggcgcaa
     7861 tcctaggaaa ataaagggtc tgcggagttt tcgtaaaatc gtaaaaatat aaaattctat
     7921 gtattttaat gcattttaat gcgaaaagtg tgtagtaact gtgtagtaac caccccaaaa
     7981 agtgtgtagt aaaaattgta tatagaaaag ccattatatg acacaaatat gagaagaaca
     8041 tggaaatgtt cttctctttt tttatgccac aatttaggca taaggagatg atgttatgtt
     8101 tgatgatgaa gtaagagaac aaatatttgc taaaagcgag ttacagaaaa tcgacttaat
     8161 gacattatcc cttgtcatta aagcgataga ggaagttttg gaggaaaata aagatgaaca
     8221 tgccgtatca gcaaccaacg atgaattata cacctaatta tggagcgtat cagtacaacc
     8281 caatggcgag ctatcagaga taccaacagc ccgagccaac acaaggcata agtggtagag
     8341 tagtacaagc agttgagact attaatccca atgaggtgcc aatggatggc agtgtagcat
     8401 tttttccaaa acaggattta acagaaatat atgctaagag ctggaatgct gacggaacaa
     8461 tacgcacatt gacttttaaa ccggttctaa atggtaagac agacatttta tcgggtgaca
     8521 cggaaaagct tgaatttgac ctatcagaga aagccacaga ggacattatg gcaaagctca
     8581 acgaactatc tgagaaaatt gagcaattat ctttaggggc gcaaagaaaa actccacgaa
     8641 cacaaagtaa ggagagtgaa aaagcatgaa tgtaatggga ataatgcaac agataatgag
     8701 caataatcgc gtaatgggaa atccaatgat taagaatgca atgagcatgg ctcaaagtgg
     8761 aaacagcaaa ggaattgagc aaatggcaag aaacctatgc aaagaaaagg gcattaaccc
     8821 tgatgatgta atgaagcaga ttagaggtaa ttttgggata tagcatatta gagaacgtgc
     8881 gcacggctct ttatgaaata aattttggag gtaaaacaga tgttcaacac aggaaattgt
     8941 ccaagcgtac ccatcgtggc gaatttggac ggaaacaacg gaaataactg gaatgacggc
     9001 tcatggcttt ggttccttat cgtagtattt gcgatattcg gaggctgggg taacggcttt
     9061 ggtggtttcg gtggcgctaa tggcggtgtc ggcagtgaaa ttcagagagg ctttgacaat
     9121 caggcagtta tcagcaagtt agacggcatt tccaacggac tttgtgacgg cttttatgcc
     9181 atgaacaaca gtatgctcac aggttttaat ggtattaacg caaatatcat gcagacaggc
     9241 tacggcatac aacaggcggt aaacgctgac acagttgcta atatgcagaa taccaacgct
     9301 ttacagtcac agcttgctaa ctgctgctgc gagacaagag aagccatcca aggtgtaaac
     9361 tacaacatgg ctactaacac ttgtgcttta cagaacacaa tgaacaataa tacaagagat
     9421 attattgaca gccagcaggc aggctttaga agcatattag actacttatg ccaggataag
     9481 atagcaacac ttacagcaga gaacaatgat ttacgtagag ctgcttcaca ggataggcag
     9541 aacgcacttc tgactactac aatggcagcg cagacaaatc agattattga tgcagtaaga
     9601 cctacaccgg taccatcatt cccggcaagc aacctttatg gatatgctta taacggatgc
     9661 ggatgtaata caggttgcgg atgctaaaca attaaataat tgagtatctt aatcgagttc
     9721 tttcgagttt ctttcgagtt tccactcgaa gaattgaata caagattatg tctgctaagc
     9781 agtattactt ataacccaag ggcagactat aatgtttgcc cttattttgt gaaagagagg
     9841 taataaaaat ggctgaattt tcaagcattg caacacagac agttgcagta aatgggaatg
     9901 tattatttac agatgcgcca acatctgtat gcaataaagg atatatttcg cacagaacag
     9961 ggagcggatt aattaacctt aaaggggcta ccaacacttg caaagcaaag tacagagtag
    10021 aatttaacgg aaatattgca gttcctacag gcggaaccgc aggagcaatt tcattagcta
    10081 ttgctgtcga gggcgagcca gacttatcta cactggcaat ctctacacca acagcagttg
    10141 aagcatttaa caatgtgtct atggcaacag atgtatggct tccttgcggt tgctgtcagg
    10201 caatttctgt caaaaacaca tctacacagg aaattagtgt agcaaatgcg aatattacag
    10261 taaatcgtat aggttaggag gtttaatatt atgcataaat gggctaaaca gataatggaa
    10321 tgcgtcaagg ctaaagttga cggaattgga attgataatt ttgagggaca aaaccttgac
    10381 gatttaaagg attttaccga gattgttaag aacatcgtag agtttgacaa ggaatatctg
    10441 attgttgaag ctatggaaaa ttcaaaagac gattacagga gatacaccga gccattatat
    10501 cacatgccgg taaactacaa cgacatggag tatatgcgtg acatggacaa gagccgaggt
    10561 aagatgtact actctgaacc aattgcacca catgtgagtg aaagcaatta tgacagagca
    10621 aagagacatt atactgaaac gaaggaaatg cacaaaggag cttcaacaga ggacaaagag
    10681 cataaaatga aagcccttga catgtatatc cgtgaattaa gcggagatat atcggagctt
    10741 ttaaacgaca tgacacctga tgaacgcaac cttttgcgca ccaagatgag caatcttgca
    10801 tcaaaactgt aattattaag gctatgggta gtaatgctca tagccatttt tagagggcat
    10861 aagcatggat ataagagtta atgatatatt gtggcgcata caattcaaaa agcccacatc
    10921 gagcgaatta aagcggtctg acggcacaat aagtttggga gtaaccgaca acacaaccaa
    10981 gacaataacg atagctgata atgtgtctga ttacatggcc gacaagatac tatgtcacga
    11041 gctagtgcat gtgtactcgt tctcatacgg ctgtgacatt gacatagaaa cagaagaaat
    11101 aatcgcagac tttatgagcc tgtacggacg gaatattgta tacacggctg acaaaatatt
    11161 taatttattg gagcaaaaat atggataaaa tagacagact attagaatac atacaccgga
    11221 ctaatccgga aatgacacgg cagaaattga ttgagaaact aggagagagc gactacagtg
    11281 ccaagagcat ttactttttg gcaattcaaa attcaaaaaa atcctaaaat attttgataa
    11341 ccccctacct tttgaatttt tcaatttcaa aaatccgttc gcaaaatttt acaaaaactt
    11401 gccgagaact tgcaaagaac tcgcaccaca ctttaattaa gtgaagtttt ctgaaaattc
    11461 aaacattttc cgtgagttgg tgcgcctgac ttgtaacatg tcacacccgg cacggcttga
    11521 cggctcgaac ctctacagat atattacaaa gcattgtaaa cggcttattt tacggcttat
    11581 tatagcgcac tcgataaaat ccatgttagc acgctcaaaa acccttaaat cgtcaaatac
    11641 atggcttaaa tgtgtatatc ataaaatcat aaaccggttt tgttaatttg tcaatgtact
    11701 acacccggac ttatagccgg acaacttgcg acagctcgat agcacgtcaa aaagggatat
    11761 aaaaatatcc ctagtggtaa cacgtgatat attttccggc cacgtagtca caaaataacg
    11821 tgaccgggtg aacgtgtgcg cgcttttcta cgacttgcaa ccattcacca cccctttgaa
    11881 ctgtgatttt taactcgtac gactccatcc attctataca atcgtacttg atataactaa
    11941 agtcacttat tttcggcact tcatagccta gcgcatgaac gcgcttatat atttcttttt
    12001 tccctaaata ctcataatta gacataatac gcccccctat ctataacaag ccttaattat
    12061 tggacttata tagtttttat gctgtagata attggagaaa gccgtccggc ggtattcctt
    12121 gccactaata agcgtggtaa tatcgttaca cgttcccgac tctgcgacag ctctaaaaat
    12181 atctgttatt gctttacgtg tggcgcgctc gcttgcctga tagcctgata catccgcata
    12241 ttttccattg tagcgtgctc ttatttcacg ttctacagcg tcaagcgtag ttagttcgtt
    12301 atcgttcatc catcaaccct cttttctatt cgtgcgtggt ttataggtta ctttttgacc
    12361 ttttcgcggt tcatacgtgc gttaatctgt ttttattagg tggtaacgca aagcgcctat
    12421 aaagggcgca caattatttg ttcaggcgtt gcacctcttg agcctgatat aaatataaag
    12481 gcatttataa gacctcttga cgcgattatt taccggacgc gcggacggag tgcaatatat
    12541 acagccgtaa agccgtataa aagcaccaat gaataaaata attaaattga taatataaga
    12601 cctgaaaagc cttatatata aagctaatag ccggaatcga accggctgaa atacccttgt
    12661 taatttgtat tgctattagc ttgcgaatta ttccaatatc atcccttatt gttcgatgat
    12721 ttcaaagcat ttttgtatct cttctaggct gtgacagcat tccccaccgg gataacgata
    12781 tatagccata taatcaccac cacctagagg ctgcatatct ttcaaatatg ctctatatcc
    12841 tccattacct tttataattt tgggatatcc gtcttttatc attttctcaa ttcttgtcat
    12901 tttcttattt ctcctaatta aataaaaatt aagtcgatag tatcagttga gttatgcgac
    12961 tttttgttga aatatgtaac ttactaattg caatttaata aaaaaataaa aacaaaccgc
    13021 cgtaaccaat tattaggcat gacgcaaaaa gcccgaaagc ctttaaaagt tcgattaaat
    13081 ctctcatatt gtgcccccta acaataacaa aaatcacctt gtagcccggt tgtaataatc
    13141 atttttccat ctttacggcg gtaaactaca ccgcaaccgc catcacttaa agaccataca
    13201 agccagccgg ccggagttat tttttcatgc ttcttataat cataaaaagc ataatgcggt
    13261 tttattccgc ttttttcctg ttcaagtgca ttgtttataa tttcatcgtc cattaataac
    13321 aacgcttttc cgtttttctg tcgtccgcaa tatctcatat atttacacct cgctaatttg
    13381 ctaaaatctg tcttgctgta ttaaatacat aaagccggtt gaagaagtgg cgcttgaagt
    13441 ctccattttc tgcaattgtg cgcccgatat tttcatattt gagacttaca accgttaagt
    13501 atttttctag cagttcatcc gggcatttta ggcactcaat agcgttttct attgttcttt
    13561 tattactatt gcagtgtatg ccctcaatac gcatttcttt ttctttttgc agtttgtcaa
    13621 attctttcag tagttccgct tttgtcatat aatcaaccat ccttttttac ttaattccaa
    13681 ttaattgcta aatgctcaaa agtctttaac tgtaaacttg tctatatctt ttcca
//
LOCUS       DAOOOL010000071        13684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_403_length_13684_cov_234.663805, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000071 DAOOOL010000000
VERSION     DAOOOL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13684
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_13684_cov_234.663805"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="rpoB"
                     /locus_tag="PKU82_07180"
     CDS             <1..665
                     /gene="rpoB"
                     /locus_tag="PKU82_07180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOZ82691.1"
                     /translation="AGRHGNKGIVAKIVREEEMPFLEDGTPVDIVLNPLGVPSRMNLG
                     QIYETVLGWAGQKLGVKFATPIFDGATLDEIEHYVDKAGLSKYGATYLTDGGTGERFD
                     QPATVGIIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEA
                     FGASNILQELLTVKSDDVVGRAKAYEAIVKGDNMPTPGVPESFNVLLHELRGLGLKIT
                     LD"
     gene            735..5030
                     /gene="rpoC"
                     /locus_tag="PKU82_07185"
     CDS             735..5030
                     /gene="rpoC"
                     /locus_tag="PKU82_07185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOZ82692.1"
                     /translation="MAAKRDNKIKSNFSRIIISLASPEHILEQSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGLPTKKLDLIIYYERYVVIQAGIKAQDGVNYLDFLT
                     EEEYLNILDNLPKENQHLDDTDPNKFIARMGADALYDLLSRLDLDELSYKLRHQASNE
                     TSQQRKNEALKRLNVVEAFRNANQNIENRPEWMIVKVVPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLLEIKAPDVILRNEKRMLQEAVDSLFDNSRKVNAVKTES
                     NRALKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPEMKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSAKKIVDRKDAIVWDILENVLKGHPVLLNRAPTLHRLGIQA
                     FQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAILEAQMLMLASHNILNPA
                     NGAPITVPSQDMVLGLYYMTKGKKSTDKEKVKGEGSVFYSPEEVTIAFNEGRVDLHAY
                     IRVKTFIKENDEIVKRVIETTVGRILFNEVVPRQAGYINDVLTKKTLRDIISQVLKTC
                     GIAQTARFLDDIKDLGFKMAFKGGLSFNLNDVLIPGAKAELVTKAQSEVEEVRNSYNM
                     GFITNNERYNQIIDIWTRTNTRITETLMKQLIADKQGFNAVYMMLDSGARGSKEQIRQ
                     LGGMRGLMAKPQKSGASGGEIIENPILSNFKEGLSILEYFISTHGARKGLADTALKTA
                     DAGYLTRRLVDVAQDVIITEPDCGTLRGLSETALKNQEEVVETLYDRILGRVSCHDIY
                     HPLTGELVVRAGDEIKEDVATQIEESPLESVEIRSVLTCESKQGVCAKCYGRNLATGR
                     MVQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGVASKGASESRHEAKFGGVIELEEMRT
                     LPYKEGDNKYEVVVGRSGEFRIVDPTTKAILATNNIPYGSHLFVKNGDTLKKGDVICE
                     WDPYNAVIICEADGKIGYEHLEEGVTFREESDEQTGYKEKVVIDSRDKTKNPVIKILN
                     DKKEVVKAYNIPVGAHIVVNEGQKIKAGNVLVKIVRASGKTGDITGGLPRVTELFEAR
                     NPSNPAVVSEIDGVVAYGGIKRGNREIIITSKEGDVKKYLVPLSKHILVQDNDFIRAG
                     APLSDGAISPSDILAIKGPSQVQEYLVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVV
                     IEDAGDTRFLERESVNKIEFMEENDWIMDKMVVTEPGDSEYRPGQIITLRKLRDDNSM
                     LKRKDQKEIEARNAIPATSSPLLQGITQASLHTKSWISAASFQETTKVLNEAAINGKV
                     DYLNGLKENVIVGHLIPAGTGLREYERLIVGSQEEYDLLMASKESSSEQPINM"
     gene            5236..6405
                     /locus_tag="PKU82_07190"
     CDS             5236..6405
                     /locus_tag="PKU82_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82693.1"
                     /translation="MLKKLPVILVFIDWYKPAYKGGGIISCCDALVEHLKDRYCFKII
                     TRNADLGSTKQLEGIKSDCWVQRTENVEVFYLSKDKIGVFNMKSLIESTPHDLLYING
                     VFSFYFSLLPLIINKWRKLQHLTLVAPMGMLCLGALKFKNIKKTLYLKTAKLLNLYSD
                     ISWHLSSEYEKNDLEKVLGSEGEFYVAPYLFNPPQTEYKTAKKENNLLRMVFISRISP
                     KKNLLACFRYLSQVDLNVKVIFDVYGPVEDESYWNECLNQMNKLGKNIQCSYRGELKA
                     EEVIKTFQSYNVSILPTFSENFGQVIPESLLAACPVIISDQTPWNKLEEHSAGWVFSL
                     KEENRFVDIICTLAKMTNEQFKVYSEGAYQYAKGFLDSEQLVLESVRMFDAKLNQ"
     gene            6551..7177
                     /locus_tag="PKU82_07195"
     CDS             6551..7177
                     /locus_tag="PKU82_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOZ82694.1"
                     /translation="MELKKIFENNQKWIDRKLATDAQYFEKLCKGQSPEVLFVGCSDS
                     RVTAEELMGSQPGEVFVHRNVSNMIISIDLNMNAVVDYAVNFLKVKHIVICGHYFCGG
                     VKAAMQAQDLGILNPWLRNIRDVYRIHKIELNNIPDENDRYNRLVELNVQEQCINLIK
                     IASVQKAYKQRNLQVHGWVFDMQTGKLIDLKIDLPKELKDIMEIYDLG"
     gene            complement(7318..7391)
                     /locus_tag="PKU82_07200"
     tRNA            complement(7318..7391)
                     /locus_tag="PKU82_07200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7355..7357),aa:Arg,seq:acg)
     gene            complement(7443..8414)
                     /gene="floA"
                     /locus_tag="PKU82_07205"
     CDS             complement(7443..8414)
                     /gene="floA"
                     /locus_tag="PKU82_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681040.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOZ82695.1"
                     /translation="MASIPLLILVVAGIILFFLFLYFVPVNLWITATFSGVKVSILNL
                     ILMRFRKVPPSVIVNAMITATKAGLSVTIPDLETHYLAGGNVNNVIKALISADKANIP
                     LSFKMAAAIDLAGRDVADAVQLSVNPRVIDTPPVSAVGKNGIQLVVRARVTVRTNINQ
                     LVGGAGEETILARVGEGIVTTIGSAHDHKSVLENPDQISKTVLNKGLDAGTAFEILSI
                     DIADIDVGENIGAKLQMEQASADLKVAQAMAEERRAAAVALEQEMIAKAQEARAKVIE
                     AEAQIPMAMAEAFRSGKLGIMDYYKMENIQADTSMRESIGKPPKGNS"
     gene            complement(8433..8897)
                     /locus_tag="PKU82_07210"
     CDS             complement(8433..8897)
                     /locus_tag="PKU82_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOZ82696.1"
                     /translation="MGTLFTIILLILAGIVLLILEILILPGLIAGIIGALMILGGIWY
                     SFHELGNTAGTIVALSTLTVTLSTIYLAFRYNVWRRFSLQQSSDSKIERVDEMDIKSG
                     DHGKTLSALRPMGTAVFHNKKVEVQSLGEMIEANATVEVIEVLANKLIVKKI"
     gene            complement(8997..9788)
                     /locus_tag="PKU82_07215"
     CDS             complement(8997..9788)
                     /locus_tag="PKU82_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOZ82697.1"
                     /translation="MEFIYSQRWCELVFENRNKNYGAYHLRQNESSRILHSWLIAITI
                     IATAIFGIAISGGPSVELIQQYSDKTQQSEIIYNFNKVTPPPLPSASTSIAVKSNGNG
                     IPVVTDRKIQTIEPLITTPVTTVPTLNSTGSGTSIVPFSSGSGTTDAGTTVTTTSDFT
                     LMPEVFPEFPGGEEALIRYVQKNIIFPHDLIRRDISGTVYVGFIIDKSGHVSNIKILK
                     GIDNAPELSVEAIRVLEKSPVWKPGLQGGKPVAVAYTLPVTFTTK"
     gene            complement(9947..10900)
                     /locus_tag="PKU82_07220"
     CDS             complement(9947..10900)
                     /locus_tag="PKU82_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOZ82698.1"
                     /translation="MSFVLKTNQAAYLLDPDNDSLVLKRKGKWSSVYLGYRVEDKLPV
                     VIKKLHNPNEMQTQLRFQREALMGFNFDGVQHTLDAWKDESGYYIIKEFIDGLSLRQL
                     MQHDIKNRTQFSIKCILATLDILEKIHSSGIIHADLRPDNILILVNKRGNPDLLNPSI
                     RIIDFGLSINMSEPVATQRIPFSLLYSPPEQLLNLPELINPSTDLFSLGLTLYESVAK
                     YPPFFNENPEMIMHMQLNTAVAEHTRIHPLLLAFIRKATFKKQLRLPPSQLSIDEIRQ
                     TVAEGQQDRFTDCNQMKTILQEVAPNISETENKSFLKRLFN"
     gene            10961..11815
                     /locus_tag="PKU82_07225"
     CDS             10961..11815
                     /locus_tag="PKU82_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOZ82699.1"
                     /translation="MKDALKLAFSPCPNDTFMFHAWVEGLIDTQGHQFEIKMADVEEL
                     NQSAMKEDADITKLSFSAFAHVADRYELLTSGAALGHNCGPLLVSKHNFKWEELSQVK
                     IVIPGKYTTANLLLTMFAPDAKNKKELVFSDIESEVMNERADAGLIIHESRFTYHQHG
                     LKKIADLGELWENKYKCPLPLGCIAVKKSLPPEVKIQLNRLMHDSIAYAMKYPKASAD
                     FIREHAQEMDENVQQQHISLYVNQWSLELGEIGKKAIRRMMEEGTIAGLLPNTEVVFV
                     DELKKLVN"
     gene            11819..12784
                     /gene="rfaD"
                     /locus_tag="PKU82_07230"
     CDS             11819..12784
                     /gene="rfaD"
                     /locus_tag="PKU82_07230"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOZ82700.1"
                     /translation="MIVVTGAAGFVGSCLVKKLNEENFNDLILVDDFSNEHKNRNLEG
                     KKFTGKVDRRQFFKWLDENHQLTQFIFHIGARTDTTEFNKKIFDELNVEYSKKVWQKC
                     VEYGLPLVYASSAATYGGGEAGYDDDENRINQLKPLNPYGDSKNEFDKWALQQKEKPY
                     FWAGLKFFNVYGPNEFHKGRMASVIFHGFNQVQKDGVIRLFKSHRPEYKDGWQLRDFI
                     YVKDVTSVCFFLMNHRKNSGIYNLGTGKARSFYDLASSTFKAMDIECNIQFVDMPIDI
                     RDKYQYFTEAKMEKLSAIGYKEPFYSLEDGVSDYVCNYLLPAKYY"
     gene            12843..13301
                     /locus_tag="PKU82_07235"
     CDS             12843..13301
                     /locus_tag="PKU82_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOZ82701.1"
                     /translation="MIKFTMRVLNLKVVRYFFSAASATIVDVGIYFLAFNYLFEKKDI
                     VFSFYTFSAPTVSLMLSYSCGLLTNFFLTKTLVFKESDLKGHHQFLRFVLVAIGVLFL
                     NYLLMKFLIRQMDWYPTIARAFSAVSIGLISFVVHKSFSFRVSATEEVED"
     gene            complement(13306..>13684)
                     /locus_tag="PKU82_07240"
     CDS             complement(13306..>13684)
                     /locus_tag="PKU82_07240"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82702.1"
                     /translation="QDCHGDWGGTAYLDSCGICVGGYTGLWDCDTINAINIITWYNEI
                     NIYPNPLSGNDINIKAGNYSGNISISITDLTGKLIFTSDCYMEKGKCLVNINPMPVDG
                     LYLFKIQFNGTMKVVKRLSIFGQ"
BASE COUNT         4398 a         2244 c         2820 g         4222 t
ORIGIN      
        1 tggcaggccg tcatggtaat aaaggtattg ttgctaagat tgtccgtgaa gaggaaatgc
       61 ctttccttga agacggaaca cctgttgaca ttgtacttaa tccgcttggt gtaccttccc
      121 gtatgaacct tggtcagatt tatgaaactg tacttggatg ggcaggacaa aagttaggtg
      181 taaagtttgc aacaccaata tttgatggag caacattaga tgaaattgaa cattatgtag
      241 ataaagcagg attatcgaaa tatggtgcta cataccttac agatggtggc acaggagaac
      301 gcttcgatca gcctgcaaca gttggtatca tctatatgct taagttaggt cacatggttg
      361 atgataagat gcatgcccgt tcaataggac catactcact catcacgcaa cagcctttag
      421 gtggtaaagc acagtttggt ggtcagcgtt tcggtgagat ggaggtatgg gcacttgaag
      481 cattcggtgc atcaaacatt cttcaggaat tgttgacagt taagtctgat gacgtagttg
      541 gaagagctaa agcttatgaa gcaattgtta aaggagataa catgcctaca ccgggagtac
      601 ctgagtcgtt caatgtatta ctgcatgaat tgcgtggatt aggtttaaaa ataacattag
      661 attaaaagaa catgtgcagg gtttttgctc tgcacataac ttttctatca taaaaattat
      721 tcaacaaaaa caaaatggca gcaaaacgcg ataataaaat aaaaagtaat ttttcacgca
      781 tcatcatcag cttagcttca ccggaacaca ttctggagca gtcaagcggt gaagtgctga
      841 aaccagaaac aattaactac cgcacgtata aaccagagcg tgatggtttg ttttgcgaac
      901 gcattttcgg tcctgtaaaa gactgggaat gtcattgcgg caagtacaag cgtatccgtt
      961 ataaaggaat tgtttgtgac cgttgcggtg tagaagtaac agaaaagaaa gttagacgtg
     1021 agcgcatggg acacatacag ttagttgttc ctgttgcaca catctggtat ttccgttctt
     1081 tacctaataa aatcggttat ctattaggat tacctacaaa gaaattagat ctgattattt
     1141 actacgaacg ttatgtagta attcaggcag gtataaaagc acaggatggt gtaaactatc
     1201 ttgacttcct caccgaagaa gagtacctga atattcttga caaccttcca aaagaaaatc
     1261 agcatcttga cgatactgat ccgaataaat ttattgcaag aatgggagca gatgctttgt
     1321 atgacctgct ttcacgcctc gaccttgatg agttatccta taaactacgt catcaggcaa
     1381 gcaatgaaac ttcgcagcaa cgtaagaacg aagcattaaa gagactcaac gtagttgaag
     1441 ctttccgtaa tgcgaatcag aatattgaaa accgtcctga gtggatgata gtaaaagttg
     1501 ttcctgttat tccaccggaa cttcgtccat tggttccatt ggatggcgga cgttttgcaa
     1561 catccgatct gaatgacctt tacagacgtg taattattcg taacaaccgt ttgaagcgat
     1621 tgcttgaaat caaagctcct gatgtgattt tgcgtaatga aaagcgtatg ttgcaggaag
     1681 cagtagattc tcttttcgat aattcacgca aagtgaatgc cgttaaaact gaatcaaacc
     1741 gtgcattaaa atcactttca gattcattaa aaggtaaaca aggacgtttc cgtcagaacc
     1801 ttcttggtaa acgtgttgat tattctgcac gttcggtaat tgttgttggt cctgaaatga
     1861 aattgcatga gtgcggatta ccgaaagata tggcagcaga attattcaag ccatttatta
     1921 tccgtaaact tattgaaaga ggaattgtaa agacagttaa gtctgctaag aaaattgtag
     1981 atcgtaaaga tgctatagtt tgggatattc ttgaaaacgt attaaaagga catcctgtat
     2041 tacttaaccg tgctcctaca cttcaccgtc tcggtattca ggcattccag ccaaaactta
     2101 tcgaaggaaa agcaattcag ttacatccat tggtgtgtac agcattcaac gctgactttg
     2161 acggtgacca gatggctgtt cacgttcccc ttggaaacgc tgcaattctt gaagcacaaa
     2221 tgctgatgct tgcatcgcac aacattctta accctgccaa tggtgcacca attacagttc
     2281 cttcacagga catggttctt ggcttgtatt acatgactaa gggtaagaaa tcaacagata
     2341 aagaaaaagt aaaaggagaa ggttctgttt tctattctcc tgaagaggtt accattgcgt
     2401 tcaatgaagg ccgtgttgat ttacatgcat acataagagt aaaaacattc atcaaagaaa
     2461 atgatgaaat tgtaaaacga gtgatagaaa ccactgttgg acgtatattg ttcaacgaag
     2521 tggttccacg tcaggcagga tatattaacg atgtacttac aaaaaagaca ttgcgtgata
     2581 tcatcagcca ggtattgaag acctgtggaa ttgcgcagac agcacgtttc cttgatgata
     2641 taaaagatct tggatttaag atggcattta aaggtggtct ttctttcaac cttaacgatg
     2701 tattgattcc tggtgcaaaa gctgaattgg ttactaaagc acagtcagaa gtagaagagg
     2761 ttcgtaacag ctacaacatg ggattcatta ccaataacga gcgttacaat cagattattg
     2821 atatctggac acgtaccaat acacgtatta cagaaacgtt gatgaagcaa ttaattgctg
     2881 ataaacaagg tttcaacgca gtgtatatga tgcttgattc cggtgcgcgt ggttctaaag
     2941 agcagattcg tcagttaggc ggaatgaggg gattgatggc aaagccgcaa aagtcaggtg
     3001 catcaggtgg tgagattatc gagaatccga ttctttcaaa ctttaaagaa ggattatcaa
     3061 ttcttgaata ctttatctca acacacggtg cgcgtaaagg tcttgccgat acagcgttaa
     3121 agactgccga tgcaggttat ctgaccagaa ggcttgttga cgtagcacaa gatgttatca
     3181 tcacagaacc tgattgcgga acattacgtg gtctttctga aacagcattg aaaaatcagg
     3241 aagaagttgt tgagacatta tatgacagaa ttcttggacg tgtatcgtgt cacgatatct
     3301 atcacccatt aaccggtgaa ttggttgttc gtgcaggtga tgaaataaaa gaggacgttg
     3361 caacacaaat agaagaatca ccattagaat ccgtagaaat cagatcagta ttgacctgcg
     3421 aatcgaaaca aggtgtatgt gctaaatgct atggcagaaa cttagctaca ggacgcatgg
     3481 ttcagaaagg tgaggctgtt ggtgttattg cagcacagtc cattggtgag ccgggtacac
     3541 agcttacact tcgtactttc cacgtgggtg gtgttgcatc aaaaggtgca tcagagtcac
     3601 gacatgaggc gaagtttggt ggtgttatcg aacttgaaga aatgcgtacg ctgccttata
     3661 aagaaggcga taataaatat gaagttgttg ttggtcgttc gggtgaattc cgaattgttg
     3721 acccaacaac taaagcaata ttagcaacga acaacattcc ttatggatca catctgtttg
     3781 ttaaaaatgg cgacactttg aaaaaaggcg atgtgatttg cgaatgggat ccatacaatg
     3841 cggtaatcat ttgcgaagct gatggtaaaa ttggctatga acatcttgaa gaaggtgtta
     3901 cattccgtga agagtctgat gagcaaacag gttacaaaga gaaagttgtc attgacagcc
     3961 gcgataaaac aaagaaccct gttattaaga ttcttaacga caagaaagaa gttgtaaaag
     4021 catataacat tcctgttggt gctcacattg tggtgaatga aggtcagaaa attaaggcag
     4081 gaaatgtatt ggtgaaaatt gttcgtgcaa gtggtaagac tggtgacatc accggtggtt
     4141 tgccacgtgt aacagaattg tttgaagcaa gaaatccatc gaatcctgct gtagtaagtg
     4201 agattgacgg agtagtagct tatggcggaa ttaaacgtgg taaccgtgag attatcatca
     4261 catctaaaga aggagatgta aagaaatatc ttgttccgtt atcaaaacac attcttgtac
     4321 aggacaacga ctttatccgt gcaggtgctc cattatctga tggtgcaatc agcccatccg
     4381 acattcttgc aatcaaagga ccatcgcaag tacaggaata cttagtaaac gaggtgcagg
     4441 aagtgtatcg tttgcagggt gtgaagatta acgataagca ctttgaagtt atcgttcgtc
     4501 agatgatgcg taaggtagta attgaagatg caggagatac acgcttcctc gagagagagt
     4561 ctgtaaacaa gattgagttt atggaggaga acgattggat tatggataag atggtggtaa
     4621 cagaacccgg tgattcagaa tatcgtccag gtcagattat tacattacgt aaattacgtg
     4681 acgacaactc aatgcttaag cgcaaagatc aaaaagagat tgaagcacgt aatgctattc
     4741 ctgctacatc atcaccattg ttgcagggta tcactcaggc atcattgcat actaagagtt
     4801 ggatatcggc agcatcattc caggaaacaa ctaaagttct taatgaagca gcaattaatg
     4861 gaaaagttga ttatctgaac ggcttgaaag aaaacgttat cgttggtcat ttaattcctg
     4921 caggaacagg gttacgtgaa tatgagcgtt tgattgttgg ctcacaagaa gagtatgact
     4981 tgcttatggc ttcaaaagaa tcaagcagcg agcagcctat taatatgtaa aatttagata
     5041 gagcatattt aaacaaacac ctttcagacc caagtctgaa aggtgtttta cttttaacag
     5101 gtagttagtt tttgaaattg tatgctctaa tttgattata caattttaaa atgtaataga
     5161 taaaattttt attttcgtat tttgttaacc caaatgtaaa taaccattgg taggttagtt
     5221 ttagagatta gaaaaatgct aaaaaaatta cctgttatcc ttgtgtttat tgactggtat
     5281 aaaccagcat acaaaggtgg aggtataatt tcctgttgtg atgcattagt agagcatctg
     5341 aaagacagat attgctttaa aataattaca cgaaacgcag acttgggttc caccaaacag
     5401 cttgaaggaa ttaaaagtga ttgttgggtt caacgtactg aaaatgtaga agtattttat
     5461 ctttcaaaag ataagattgg tgttttcaat atgaaatcat tgattgaaag tacgccacat
     5521 gacctattat acattaatgg ggtattttcg ttttatttta gtttattgcc tttaataatc
     5581 aataagtggc gtaaacttca acacctaaca ttggttgctc caatggggat gttatgttta
     5641 ggagcactta aattcaagaa tattaaaaag actctttatc ttaaaacagc taagttgctt
     5701 aatttgtatt cggatatttc gtggcatctg tcatcagaat atgaaaagaa tgaccttgag
     5761 aaagtgttgg gaagcgaagg agaattttat gttgctccct atttatttaa tccgccacaa
     5821 acagaatata aaacggcaaa gaaggaaaat aatttgctgc gaatggtttt tatttcacgg
     5881 atatcaccca aaaaaaatct tttagcttgc tttcgttatt tatcacaagt tgatttaaat
     5941 gtgaaagtga tttttgatgt ttatggcccg gtagaagatg aatcttattg gaatgaatgt
     6001 ttgaatcaga tgaataaact tggaaaaaac attcaatgta gctaccgtgg agaattgaaa
     6061 gctgaagagg ttataaaaac atttcaaagt tataatgttt ctattttacc aactttcagc
     6121 gaaaactttg ggcaggttat accggaatct ttacttgctg catgtcctgt aattataagc
     6181 gatcaaacac catggaataa attggaagag cattctgccg ggtgggtgtt ttctttaaaa
     6241 gaagaaaata ggtttgtgga tataatatgc actcttgcga aaatgacaaa tgagcaattt
     6301 aaggtttatt cggaaggagc ttatcaatat gcaaaaggct ttttagatag tgaacaatta
     6361 gtgttggagt ctgtaagaat gtttgatgct aagctgaatc aataattgtt ttctatattt
     6421 tatttttaat gtataatgaa atatatgatg acaaattcat gcgttagttt atccagaatg
     6481 atagttggat ttattgctgt taaaattcaa gcaaggaggt aacaatattt tttattatta
     6541 aatttgtaca atggaactta aaaaaatatt tgaaaataat cagaaatgga ttgataggaa
     6601 actggccact gatgcgcaat attttgaaaa attgtgcaaa ggtcaaagtc ctgaagtttt
     6661 atttgtaggt tgttctgaca gcagggtaac agcagaggag ttaatgggat cgcagcccgg
     6721 tgaagtattt gtacatcgca atgtttcaaa tatgattatc agcattgatt taaacatgaa
     6781 tgctgttgta gattatgctg tgaatttttt aaaagtgaaa cacatcgtaa tctgtggcca
     6841 ttatttttgt ggaggagtaa aggctgctat gcaggcacag gatttaggca tactcaatcc
     6901 atggctaaga aatattcgtg atgtatatcg tattcataaa atagaattga ataatattcc
     6961 tgatgaaaac gatcgttata atcgcttagt ggagttgaat gttcaggaac aatgcattaa
     7021 tctgattaaa attgcatcgg ttcaaaaagc atataagcaa cgcaatttgc aagtacatgg
     7081 atgggtgttc gatatgcaaa ccggcaaatt aattgacctg aaaattgatt taccaaaaga
     7141 gttgaaagat attatggaga tctatgattt agggtaggta gttgccttaa gaatatcaaa
     7201 cattcatggt aagatttcct catattttaa atttctaatt ttcttaactg cagtacattc
     7261 agtagccata aatagaaaac agaaaagcga accattactg attcgcttta tattaagtgg
     7321 tcccggaggg attcaaaccc ccaaccttct gatccgtagt cagatgctct attcagttaa
     7381 gctacggaac cattttttta gtggcgcaaa gataaccaaa ttagcttatt acaaaagaga
     7441 aattatgaat ttccttttgg tggcttaccg attgactcac gcatagatgt gtcggcctga
     7501 atgttttcca ttttgtaata gtccattatg cctaatttac cactacgaaa agcttctgcc
     7561 atagccatag gaatttgtgc ttcggcttca ataactttgg ctcttgcctc ttgtgctttg
     7621 gcaatcattt cctgttctaa agctacggct gcagccctgc gctcttctgc cattgcctgt
     7681 gctaccttta agtctgcaga agcctgttcc atctgaagtt ttgctccaat gttttcacct
     7741 acatcaatat cggcaatgtc tatagataaa atttcaaatg ccgtgcctgc atcaagacct
     7801 ttgtttaata cagtttttga aatctgatcg ggattttcta atacactttt atggtcatgt
     7861 gccgaaccaa tagtagttac aataccttcg ccaacacgtg caagaatggt ttcctcacct
     7921 gcaccaccaa ctaactgatt gatgttggtg cgaacggtaa cacgagcacg tactaccaat
     7981 tgaattccgt ttttgcccac agcagaaaca ggaggggtgt caataactcg tggatttaca
     8041 gaaagctgaa cagcgtctgc aacatcgcga ccggccagat caattgctgc tgccatttta
     8101 aatgataagg gaatgtttgc tttatcagca gaaattagtg ctttaataac attatttaca
     8161 tttcctcctg ccagatagtg tgtttctaaa tcaggaatgg taacacttag acctgcttta
     8221 gttgcagtaa tcatagcatt aacaattaca cttggtggta cttttctgaa acgcatcagg
     8281 attaagttca ggatgctgac tttcacaccg gagaatgttg cagttatcca aaggttaaca
     8341 ggaacaaaat acaagaacaa aaagaacagg ataatacctg caacaaccag aatgagtaac
     8401 ggaatacttg ccattttttt taatttttaa gtttatattt tttttacaat aagtttgttt
     8461 gccaatactt caatcacttc aactgtagcg tttgcttcta tcatttcgcc aagtgattgc
     8521 acctctactt ttttattatg aaatactgct gtgcccatag gtctcaatgc agaaagtgtt
     8581 tttccatgat ctccggattt tatatccatt tcatcaacac gctcaatttt agaatcactt
     8641 gattgttgca ggctgaacct gcgccaaaca ttgtaacgaa atgcaagata aatggtagat
     8701 agtgttacag taagtgtact tagtgcaacg atggtgcctg cagtatttcc cagttcatga
     8761 aaggaatacc agatgccacc caaaatcatt aatgcaccaa tgatacctgc aattaatccc
     8821 ggcagaatca atatctccaa aatcaacaga actatacctg caagtatcag taaaatgatt
     8881 gtaaataagg ttcccatttt cggtgacaaa catagtaaaa aggccgttgc taaaaaacaa
     8941 cagcccctat taaaaaacac gaatattttt tatgctgatt tttgctgaat tgtaaattat
     9001 ttggtagtaa atgtcaccgg taatgtatat gcaactgcca caggtttacc tccttgtagt
     9061 cccggtttcc aaactggaga tttttcaagc actctgattg cttcaactga caattcaggt
     9121 gcgttatcaa tacctttaag aattttgata ttgctgacat gaccactttt gtcaattata
     9181 aatccaacat aaacagttcc actgatatca cgcctgatta ggtcatgtgg aaaaataata
     9241 tttttttgca cgtaacgaat taaggcttct tcacctcccg gaaattcagg aaatacttca
     9301 ggcattaatg tgaaatctga agttgtggtg actgtagttc ctgcatctgt agtgccactg
     9361 ccagatgaaa agggaacaat agaagtgccg ctaccagttg aattaagagt gggtacggta
     9421 gtaacaggtg tggttattaa aggttcaata gtttgaattt ttctatcagt cactacggga
     9481 atgccgttac cgttggactt tactgcaatt gatgtagatg cagaaggtag tggaggaggt
     9541 gttaccttgt tgaagttata aattatttca gattgttgag ttttatctga gtattgctga
     9601 ataagttcaa cagatggacc acctgatatc gcaattccaa atattgctgt tgcaataatt
     9661 gtaatggcta tcaaccaaga gtgtagaatg cggcttgatt cattttgtct taaatgatat
     9721 gcaccataat ttttgttgcg gttttcgaac accagttcgc accaccgctg tgaataaatg
     9781 aattccataa tgttgctttt atcttttaga tgtatgacgc tgcaacattc catattattt
     9841 ttagctgaaa actattttac attctaatga cggtaaaatt tgattccttc ttcaagttaa
     9901 aaatgtatat attcttacat taatttcttt taacagaatg aaaaaatcaa ttaaatagcc
     9961 ttttaagaaa actcttgttt tcagtttcag aaatatttgg tgctacttcc tgtaaaattg
    10021 ttttcatttg attgcaatct gtaaatcggt cttgctgacc ttcagcaact gtttgccgta
    10081 tttcatcaat tgataattgt gatggaggaa gacggagttg ttttttgaaa gtagctttac
    10141 gtataaaagc taaaagcaaa gggtgtattc ttgtatgctc tgcaactgca gtatttaact
    10201 gcatgtgcat tatcatctcc gggttctcat taaaaaacgg aggatactta gccacagatt
    10261 catataatgt aagtccaagg ctgaataaat ctgtagatgg atttatgagt tccggtaaat
    10321 ttaataactg ttcaggtggt gagtagagaa gtgaaaaagg tatccgctgt gttgcaacag
    10381 gctctgacat attgatggaa agtccgaagt caataattcg aatacttggg ttgagtaaat
    10441 caggattacc tcttttgtta acaagaatca gaatattatc aggtcgtaaa tcagcatgaa
    10501 taattccgct gctatgaatt ttttcgagaa tatcgagtgt ggcaagaata catttgatac
    10561 tgaattgtgt tctgtttttt atgtcatgtt gcatcagttg acgcaatgaa aggccatcaa
    10621 taaattcttt aattatataa tagccgcttt catctttcca tgcatcgagt gtatgctgta
    10681 caccatcaaa gttaaagccc atcaatgctt cacgctgaaa ccttaattgt gtttgcattt
    10741 cattcgggtt gtgtaatttt ttaatcacca ccggtaattt atcttctaca cgataaccta
    10801 aatatacaga actccatttt cctttgcgtt tcagcacaag gctatcatta tcggggtcga
    10861 gaaggtaggc tgcctgattg gttttcagaa cgaatgacat gattcagaga atatttatgg
    10921 tgggctaaat taataaacaa gctttatgta aatttgttgc atgaaagatg cacttaaatt
    10981 agcattttct ccctgtccca atgacacctt tatgtttcat gcatgggtag aaggtttaat
    11041 tgatactcaa ggtcatcagt ttgaaattaa gatggcagat gtcgaagaat taaatcagtc
    11101 agcaatgaaa gaggatgcag atattaccaa attgagtttt agcgcctttg cacatgttgc
    11161 agacaggtat gaattgctga cttcaggagc tgcacttgga cataattgcg gaccgctttt
    11221 agtaagtaag cataatttta aatgggaaga attatcacaa gtaaaaattg ttattcccgg
    11281 gaagtataca acagcaaatt tattgctgac aatgtttgca ccggatgcaa aaaataaaaa
    11341 agaattggtt ttttcagata ttgaaagtga agtgatgaat gaaagagccg atgcgggatt
    11401 gatcatacat gagagtcgtt ttacttatca tcagcatgga ttgaaaaaaa ttgctgactt
    11461 gggagaactt tgggaaaata aatataaatg cccattaccg ttgggttgta ttgcagtaaa
    11521 aaagagcctg ccaccggaag taaaaattca attaaataga ttaatgcatg atagcattgc
    11581 ttatgcaatg aaatatccga aggcttctgc tgattttatc agagagcatg cacaggagat
    11641 ggatgagaat gtgcaacagc aacatatttc attgtatgtt aatcaatggt cgttagagct
    11701 gggagagata ggaaaaaagg caatcagaag aatgatggaa gaaggcacta ttgccggatt
    11761 attaccaaat acagaagttg tatttgtaga tgagttaaaa aaattagtta attagaaaat
    11821 gatagtagtt acaggagcag caggatttgt tggaagttgt ctggtaaaga aactcaacga
    11881 agagaatttt aatgatttga ttcttgtaga tgatttcagc aatgaacata agaatagaaa
    11941 tcttgaagga aaaaaattta caggcaaagt agataggagg caatttttca agtggttaga
    12001 tgaaaatcat caacttacac agtttatttt tcatataggt gcgcggaccg atacaacaga
    12061 atttaacaaa aagatttttg atgagttaaa tgttgaatac agtaaaaagg tttggcagaa
    12121 gtgtgttgaa tatggcttgc cgcttgttta tgcatcatca gctgcaactt atggtggggg
    12181 agaagcaggc tatgatgatg atgagaatag aatcaatcag ttaaaaccat tgaatcctta
    12241 tggagattca aagaacgaat ttgataagtg ggcactgcaa caaaaagaaa agccttattt
    12301 ctgggcgggg ttaaaatttt ttaatgtata tgggccaaat gaatttcata aaggcaggat
    12361 ggcatcagtt atttttcatg gatttaatca ggtgcaaaaa gatggtgtta ttcgattatt
    12421 taaatcgcat cgccctgagt ataaagatgg ctggcaattg cgtgatttta tctatgtgaa
    12481 ggacgttaca tcagtctgtt tctttctgat gaatcatcgt aaaaattccg gtatttacaa
    12541 tcttggtact ggaaaggcaa ggagttttta tgatttagca tcaagtacat tcaaggcaat
    12601 ggatattgag tgtaatattc agtttgttga tatgccaatt gacattagag ataaatatca
    12661 gtattttaca gaggcaaaaa tggaaaaact tagtgcaatc ggatacaaag aacctttcta
    12721 ttcgcttgaa gatggtgtgt cagattatgt atgtaattat ctgttgcccg caaaatatta
    12781 ttaattcata gtttatcaac aatgattaac agcaaatcag gaaaacctat tttagcgccc
    12841 tgatgataaa attcactatg cgtgttctga acctgaaagt ggttcggtac tttttctcag
    12901 cagcatctgc aacaattgta gatgtaggaa tttatttttt ggcatttaat tatttgttcg
    12961 aaaagaagga tattgtcttt tcattttata ctttttctgc accaacagtt tctctaatgt
    13021 tgtcttattc atgcggattg ttaacgaact tttttcttac aaaaacttta gtctttaaag
    13081 aatcagattt aaaaggacat catcagtttt tgcgttttgt attggtagct attggtgtct
    13141 tgtttttgaa ttatttgctg atgaaattcc ttattcgtca gatggattgg tatcctacaa
    13201 tagcacgtgc tttttctgca gtgagtatag gactgataag ttttgttgtt cacaaaagct
    13261 tttcgttcag agtaagtgca acagaagaag tagaagatta atctgctact gtccgaatat
    13321 agagagacgt ttcactactt tcattgttcc attaaattgg attttgaaaa gataaagccc
    13381 atccacaggc attggattga tatttaccaa acacttacct ttttccatat aacaatctga
    13441 tgtgaaaatt aatttaccgg ttaagtcagt tattgaaatc gagatatttc cagaataatt
    13501 acctgcttta atattgatat catttccact gagtggatta gggtaaatgt ttatttcatt
    13561 ataccatgta atgatattaa tagcattgat agtgtcgcag tcccaaagcc ctgtgtatcc
    13621 acctacacat attccacaag aatccaaata ggcagtgcca ccccaatcac catgacaatc
    13681 ttgt
//
LOCUS       DAOOOL010000072        13668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_404_length_13668_cov_115.238228, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000072 DAOOOL010000000
VERSION     DAOOOL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13668
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_13668_cov_115.238228"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="PKU82_07245"
     CDS             <1..2172
                     /locus_tag="PKU82_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ82703.1"
                     /translation="LTVMNAGDFISEYFQRGGTAPIIVVSSQGEEKIVVELMKAGACD
                     YIPKAVLTPETIARSLKGALKSKASKNNHSNIETALVQTERTLNAVVSKSPLILFSIN
                     ESGIFTLFKGMAMDTIRLSEEDVIGKSVFEIWNRLPVRLKDVKKALLGEEVKQNMEFE
                     GRFFEVHYMPSVNSEGALTGIMGIATDITGHKEQEDELRQQIILSSEAQKMKEQFLAN
                     MSHEIRTPIHGIIGITKIVLQSQLDKDQSHYLNAIRKSADNLLVIINDILDFSKIEAE
                     KMTFESVCFNLFDLINTINELFKARVTDKSVKIIADFDQQLPKLLKGDPVRLSQVINN
                     LMGNAVKFTHKGSVTLKTELINISDTKASIRFKVIDSGIGIAEDKLATIFDSFSQAGT
                     DITRKYGGTGLGLSIAKKIVELQDGSIRVESKLNHGTTFIFEMPFDLPAPGEVETEAK
                     KVAEVHAGFNGKIKILVVEDNDINRLIINKHIKDWGFDHDEACNGLEAIEKLSANDYD
                     VILMDIEMPELNGYAATERIRTEMKGHKSQIAILAMTAHVSASEKEKCIAAGMNDYVS
                     KPFDPTEVKNKIIELSGKANESVTEQPIEEAANSQSSSQATTKLTSLSFLNEMSGGNA
                     DFIKEFLSLFLTNIPVSIDELEEGLKHKDFEKIRQSAHKMKPSLNYVGLKDTYETVAT
                     LEKYAREKSNLEDYPAMIKKISDECSVACIELESELKNVEV"
     gene            2184..3353
                     /locus_tag="PKU82_07250"
     CDS             2184..3353
                     /locus_tag="PKU82_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOZ82704.1"
                     /translation="MAKIFIVEDDAMVATLIRQSLSKNPDFEIQHFESAEECLNNLHL
                     NPDIVTIDYHLPGMNGVGLMRRIKDYNASIMCILVSGQDSLEVVVDSYKSGAQDYIIK
                     NDNLFVNLENSVKNLSMNVVLKKENEMLKDQIIDRHKYSNIMGSSNAVLRILRLIQKV
                     EKSNMMVLVTGQSGTGKELVARALHYNSPRARKNFVTVNMSAIPEELIESELFGHEKG
                     AFTDARERRIGKFEEANEGTIFLDEIGEMPLSLQAKLLRVLQEKEIQRIGSNKTIKLD
                     FRMVAATNRNLAVEVKEGRFREDLFYRIQGFLIHLPPLKERGDDIILLAKHFLDLYAQ
                     ANKTPVAQLSNEAAKFMLDYEWPGNIRELKAVMERAAILADNATISVDDLMFVNL"
     gene            3445..3738
                     /locus_tag="PKU82_07255"
     CDS             3445..3738
                     /locus_tag="PKU82_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82705.1"
                     /translation="MRKKRKKHLPNRLRQSAKLRLSYRLIAVTATVTMAVVVGGIIYF
                     NISNSKKSKASTTTSDAEQRPVDFNIPDLKQNFREETINGIRVRKMRDEHVTN"
     gene            3725..6808
                     /locus_tag="PKU82_07260"
     CDS             3725..6808
                     /locus_tag="PKU82_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82706.1"
                     /translation="MSRIRIQFLVFSLIYFFAYCNAVFAVTKTTTASGSWSNPAIWSP
                     TGVPASSDDVVISSAHNITISQQTFCKTLVVSGGAVLSLSPSKLLQINGAVNIAGTFD
                     MNGGNVKQLLNNAALQIGVTGKLIWNPGDNTLTGASLLTNSVETFNETSTLEIKKWYS
                     YATVPLASVITGNLGNVTISTQVGSTLFEWNQNNQFESHQIKGTLTINNGWVVLDKSG
                     SISDTKIGKIILPNVNSVLEFHSGSHPASFKVTTDSIANIGGEITGLLNGEGNVTLNV
                     NGGFLNMGNVELIYNKGTLNTANGNAKLNVGGKYLQTHGDLRGVFNLSSTAAGNTEFK
                     FNSLEMTGGLLFAYYACNATGALNSVTVTNDMVLNFSNASDKFRVNGLTTLAGQYSKS
                     RSLLKVDGNLILSGNAAAEFTSSGSVGAEENRIKGDVNISGLNSNFNMGSHAVILSVN
                     GDVNIDGGNTCFSKTPGVSQISLNKNFSLQSGTFILKGHEGKSELTVNGAYSQTGGVF
                     YLHSNTLTASNDSVKAYFKSSFRQWNGIINFDDNPEGGKSVLFIQGNEFIAKGSSLIT
                     RAGTGIGSVFGNLIYCTNGDLNYQRSTASVIDQIKQRITVNCRLKVTDGNLLLSSHSQ
                     PAVDFLRVESGAILNLLSTQIKSNLKYQNSGFTVDAGGLVRTANSKGFYNTLGTAAVC
                     SNGNLNYFLDRNSIVDYVGSNQLITGSGSGVINKEQFKYGVLRVSGNGILATNVEVRN
                     RIELGSSTIKLNQKTLIIENENTNALSASGGSLICASESDKLIWKNMKGGSSYTIPFS
                     SDGKVVSNVVVQPIVSGDISMYNYTGNPVSENLPDGIQPFLVDGKDVTDESLARRIIG
                     FDSQVKANITLKFFKNEIPGFKNGDTLVLIKNIGQWEVTPFSKVGDEQNPVSVQATNI
                     NANAVFALCTGIGAKMPDNTNGNNNSQGNFSVEQISPVPFNDFLKVNINAPTIGVIYL
                     TIVGSDGRELRKQMFDVSPGKNLLALSELSSLPTGVYFIQINGFKQTITKKVMRQ"
     gene            6906..8045
                     /locus_tag="PKU82_07265"
     CDS             6906..8045
                     /locus_tag="PKU82_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82707.1"
                     /translation="MARKLKLRQRQKMSIMYKAIFVTAGTSIMGLAVYLTVVMNTSNV
                     TDSRAGALTNMIQGYDVNNGEVICAFDWNGDDPTKASKGPDAIKVSPFAVVMSEGVEG
                     TNGLSAGKGKDGIDLTIPTDNYFNTDGIDISFDYKRLDDNCDFISRGRYFNFGMKNGK
                     LIIAYRVNLEKGRSYSVNETTNYELVKDNDFRNYRFVYNPNTGKGEIMVDDVVVWNYQ
                     GPKERSMFWKNSDNIVIAKNMNGGGTDKTILDNFIIRSTTHVQQLPITLLSFQAEARD
                     HHVMVSWYTSRETDIDSFVVERSVDAIEYQKIGAIKANGNSEVLRAYAFVDKAPLENK
                     IAYYRLMPSNKPLKSIAVPIIGYRYRMNHPETAFNPADYPADTVK"
     gene            complement(8096..9178)
                     /locus_tag="PKU82_07270"
     CDS             complement(8096..9178)
                     /locus_tag="PKU82_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOZ82708.1"
                     /translation="MKAKILDLYIIKKFLGTFLFAIGLIICIAVVFDISEKVDDFIER
                     HAPLKAIAFDYYLNFIPYFANLFSPLFVFISVIFFTSKMAGQTEIIAILNSGISFNRF
                     LRPYIISATVIAIASFYLNNWVIPHANLVRLQFENIYIKNPYVYRGRNIHRQIKPGEF
                     VYFESYDNKANTGFMFSIEKFEGGKLVTKLMADRITWDSLTQKWSIENYTIRHINGMT
                     EELKKGIRLDTTLSIHPSEFNRRMNFIEAMDFSELNSYIKEEKSRGSEVIPFYEVEKY
                     RRTSYPFATFILTLIGVAISSKKVRGGIGLQLGAGILLSFTYIMFMQVSTTFATNGNL
                     PAVVAVWIPNLVYAFIAIYLLRKAPK"
     gene            9316..10692
                     /locus_tag="PKU82_07275"
     CDS             9316..10692
                     /locus_tag="PKU82_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOZ82709.1"
                     /translation="MRYHFIAIAGSAMHNLALALHQLGHEVSGSDDEIVEPAKTRLAK
                     SGILPEKTGWFPEKINADIDAVILGMHARADNPELAEAIQKNIKVFSYPEFLYEHSKN
                     KTRVVIGGSHGKTTITSMILHVLKTSGNNFDFMVGAQLEGFDTMVKLSNDAPIIVLEG
                     DEYLSSPIDRRPKFHLYHAQIGLISGIAWDHINVFPTLEIYEEQFRIFAREIPRDGKL
                     FYCMNDDAVRKICEAENVVADRKGYSIPKHVIRNGKTFIYDDYNKEVPLQIFGEHNLM
                     NLEGARMICNSIGVNNEVFYNAIKSFKGAARRLELLEENDQFAVFRDFAHSPSKLKAT
                     LEAVRRQFPERKLIACMELHTFSSLNENFLDEYKGTMADADVAVVYFNPKTIEHKKLP
                     PISSEKIFQSFDRSDIKVINDSMQLINFLNTEDKQQVVYLMMSSGTFDGLNLKSFAES
                     LNTSHQKV"
     gene            complement(10693..11517)
                     /locus_tag="PKU82_07280"
     CDS             complement(10693..11517)
                     /locus_tag="PKU82_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOZ82710.1"
                     /translation="MTSPKSEWKYDLISTIFPSKFLKREIRIDYYLPAQYSKNTGVLF
                     MLDGQDAELLHLKDTLQSLTQLKKIKPIIVIAIHANKNRLQEYGVVGKADYLKRGNKA
                     KQFSKFILDELIAHCSSFTGIKFTPSKTAIAGFSLGGLTAFDLAWNHPSVFETVGVFS
                     GSFWWRKKSYEDGYTDDDRIMHQVVKKSKSAPAIKCWIQTGTKDETADRNKNGVIDSI
                     DDARDLIFEMKQKGVPEKNIQYVEIPEGQHNQITWSAALPLFLCWAFGNKRKVDIY"
     gene            complement(11536..13077)
                     /locus_tag="PKU82_07285"
     CDS             complement(11536..13077)
                     /locus_tag="PKU82_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOZ82711.1"
                     /translation="MSLTEQFKEVVNKEYQFKGDSIVLGAPVLKGETLTGSFIKAPLK
                     TFNRHGLIAGATGTGKTKTMQGIAESLSAKGVSCLLMDVKGDLSGLASPGTTNPKIDE
                     RYQKLGLTWKPLQFPVEFLTLSDEKGVRLRATVSEFGPVLFSKILDLNDTQQGVVSLI
                     FKYCDDKQMPLLDLKDFKKVLQFLTNEGKDEIKADYGAISSASASTIIRKVIEIEQQG
                     AEKFFGEKSFEVEDLLGIDENGNGKLNIIRLTDIQDKPKLFSTFMLSLLAEVYSTFPE
                     VGDPDAPKLVIFIDEAHLIFNNASKVLLDQIETVIKLIRSKGIGVFFVTQSPEDIPAA
                     VLAQLGLKVQHAFRVFTANDRKALKLIAENYPLSDYYKVDELLTTLGIGEAAITVLNE
                     KGVPTPIAHTLLCTPASRMDILTPDELTKSISKSRLTTKYNETLDRESAFEVLSKKLE
                     TQVAENTKATKAAEAKEEPSQLDQVLNSSTGKQVMRTVTSVLTRSLLGALGLGATRRK
                     KSGWL"
     gene            13386..>13668
                     /locus_tag="PKU82_07290"
     CDS             13386..>13668
                     /locus_tag="PKU82_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOZ82712.1"
                     /translation="MSEVRNPQVAIATEIYTEDSVLIGKYYHENRTPVSFDSISTYAV
                     QALVATEDVRFYKHFGLDLYALISGAYSTAKGDERGASTITQQLAKNMYS"
BASE COUNT         4509 a         2246 c         2646 g         4267 t
ORIGIN      
        1 ctgactgtga tgaatgcggg tgatttcatt tcagaatatt ttcaacgtgg tggcacagcg
       61 ccaatcattg ttgtatcatc acaaggcgaa gaaaaaatag ttgttgaact gatgaaggcc
      121 ggtgcctgcg attacattcc taaagctgta ctaacaccgg aaacaatagc acgttcctta
      181 aaaggtgcat taaaatcaaa agcatctaaa aacaatcata gcaatattga aacagcactt
      241 gtacaaacag agagaactct caatgcagtg gtttcaaagt cgccattaat tctgttcagc
      301 atcaatgagt caggcatatt taccttgttt aaaggtatgg caatggacac aatccgtttg
      361 tcagaagaag atgtaattgg aaagtctgtt tttgaaatct ggaatagact acctgtaaga
      421 ctgaaggacg ttaagaaagc attgcttgga gaagaggtta agcagaacat ggagttcgaa
      481 ggccgatttt ttgaagtgca ttacatgccc tcagttaata gcgaaggtgc tcttactgga
      541 atcatgggta ttgctactga cattacaggt cataaggaac aggaagatga attgcgacag
      601 caaattattt tatcgagtga agcacagaaa atgaaagagc agtttctggc aaatatgagc
      661 catgaaattc gtactccaat tcacggaatt attggtatca caaaaattgt gctgcaatca
      721 cagcttgata aagaccaatc gcattattta aatgcaataa gaaaatcagc agataatctt
      781 ttagtaatta tcaacgacat tctcgacttc tcgaaaattg aagctgagaa aatgacattc
      841 gaatcggtct gtttcaattt gtttgatctt attaatacca tcaatgaatt gtttaaagct
      901 cgtgtgacag ataaaagcgt taagataata gcagattttg atcagcagtt accaaagttg
      961 ttaaagggcg atccagttag actgagtcag gttattaata accttatggg taatgccgta
     1021 aagtttacac ataaaggatc tgtaacactt aaaacggaac tcattaatat cagcgacaca
     1081 aaggctagca taagatttaa agttatagac tccggtattg gcattgccga ggataaactt
     1141 gctacaatct ttgattcatt ctcgcaggct ggaacagaca ttacacgtaa gtatggcgga
     1201 acaggattgg gtttaagtat tgcaaaaaag attgtagaac tgcaggatgg aagcatacga
     1261 gttgaaagta agttgaatca tggcacaaca ttcattttcg aaatgccatt tgacttacct
     1321 gcacccggag aagtggaaac agaagcgaag aaggttgctg aagtacatgc cggttttaat
     1381 ggaaagataa aaatcctggt tgtagaagat aatgacatca atcgtttaat cataaataaa
     1441 cacattaaag attggggttt tgatcacgat gaagcttgta atggtttgga agcaatagag
     1501 aaattgtctg ccaatgacta tgatgttatt ctgatggata tagagatgcc tgaattaaat
     1561 ggttatgcag ctacagaaag aatcagaaca gaaatgaaag gtcataagtc gcagattgca
     1621 attttagcca tgacagcaca cgtttctgca agcgaaaaag aaaaatgcat tgcagcagga
     1681 atgaatgatt atgtatcgaa accatttgac cccactgaag taaagaataa aattattgaa
     1741 cttagcggca aggctaatga gtctgttaca gagcagccta ttgaagaagc tgccaatagt
     1801 caaagtagtt ctcaggccac aacaaaactg acaagtctta gttttctgaa tgaaatgtcg
     1861 ggtggcaatg cagattttat taaagagttt ctgtcattgt ttttgactaa cataccggtg
     1921 tccattgatg aacttgaaga aggtttgaaa cataaggact ttgaaaaaat caggcagtca
     1981 gcacataaaa tgaaaccttc cttgaattat gtaggtttaa aggatacgta cgaaacagtt
     2041 gcaacactag aaaagtacgc cagagaaaag tcaaatttag aggattatcc ggcaatgatt
     2101 aaaaagattt ctgatgaatg tagcgttgcc tgtattgaac ttgagagtga actaaaaaat
     2161 gtagaagtat aaataataaa actatggcta aaatttttat tgtggaagat gatgcaatgg
     2221 tggcaacact gatacgccag tcattgtcaa aaaatccaga tttcgagatt cagcattttg
     2281 aatcggcaga ggaatgttta aataatctgc atcttaaccc tgacattgta actattgact
     2341 atcacttgcc gggaatgaac ggtgtaggtc tgatgcgaag aattaaagat tataatgcat
     2401 ccatcatgtg catacttgtt tcaggacaag acagccttga agtggttgtt gattcttata
     2461 aaagcggagc acaggactac attatcaaga atgataattt gttcgtgaat cttgaaaact
     2521 cagtaaaaaa tctgagcatg aatgtggtgt tgaagaagga gaatgaaatg ctaaaagacc
     2581 agattattga ccgtcataaa tacagcaata taatgggtag cagtaatgct gtgttaagaa
     2641 ttttgcgtct gatccagaaa gttgaaaaga gcaatatgat ggtgcttgtt accggacaaa
     2701 gcggaacagg taaagaactt gttgcacgtg cgctacacta taactcgcct cgtgcacgca
     2761 aaaattttgt taccgttaac atgagtgcaa ttcctgagga acttattgaa agtgaacttt
     2821 tcggtcatga aaagggtgca tttacagatg cccgcgaacg tagaatagga aagtttgaag
     2881 aagctaatga aggaactatt tttcttgatg agataggaga gatgccttta agcttacaag
     2941 ctaaattgct tcgtgtattg caggaaaaag aaattcagcg tataggaagt aataaaacaa
     3001 ttaagctcga tttcagaatg gtggcagcca caaacagaaa tctggctgtt gaagttaaag
     3061 aaggaagatt cagagaagat ttattctaca ggattcaggg atttctgatt catttgcctc
     3121 cattgaaaga aagaggtgat gacattattc ttttggcaaa acatttcctt gacctgtatg
     3181 ctcaggcaaa taaaacacct gtggcacaac tctcaaacga agcagctaaa tttatgcttg
     3241 attatgagtg gcccggaaat attcgtgagc ttaaagccgt gatggaaaga gccgccattc
     3301 ttgcagataa tgctactatt tctgtagatg atttaatgtt tgtaaattta taaattagta
     3361 ctgagtgata ttataaggcc gcaccgtaat gcggcttttt ttatgcaacc tttttagcga
     3421 ttttacatat aaggatttac gcagatgagg aagaaaagaa agaaacacct gcccaacagg
     3481 ttaagacaaa gcgctaagct acgactgtct tatcgtttaa ttgctgttac tgcaacagtt
     3541 actatggctg ttgttgttgg cggaataatt tattttaata tctccaactc caaaaaatca
     3601 aaggcaagta ctacaacatc agatgcagag caaagaccag tggattttaa tatccctgat
     3661 ttgaaacaaa atttcagaga ggaaactatt aatggtataa gagtaagaaa gatgagggat
     3721 gagcatgtca cgaattagaa tacaatttct tgtcttttca ttgatttatt tttttgctta
     3781 ttgcaatgca gtatttgctg ttacaaaaac tacaacagct tccggtagct ggagtaatcc
     3841 tgctatttgg tcacctaccg gtgttccggc ttcttctgat gatgttgtta tttcatctgc
     3901 acataatata acaatatcac aacaaacatt ctgtaaaaca cttgttgttt ctggaggtgc
     3961 tgttcttagt ttgtcaccat ctaaattact tcagataaat ggagctgtca atattgcagg
     4021 tacatttgat atgaatggtg gtaatgttaa gcaacttctt aataatgcag cattacaaat
     4081 tggtgttaca ggaaagttga tttggaatcc gggtgataac actttaacag gagcatcgct
     4141 actaacaaat tctgttgaaa cctttaatga aacatcaacc ttagaaatca agaaatggta
     4201 ttcttatgca actgtaccat tggcgtctgt gattactggt aatttaggaa atgtcactat
     4261 aagcacacag gtgggaagta ccttgtttga atggaatcag aataatcaat tcgaaagtca
     4321 tcagattaaa ggcacattaa ctattaacaa cggctgggtt gtattggata aatcaggtag
     4381 tatttctgat actaaaattg gtaagattat tttacccaat gtaaattctg tacttgagtt
     4441 tcatagcggt agccatccag catctttcaa agtaactaca gatagtattg caaatattgg
     4501 tggtgaaata actggtcttc tcaatggtga aggcaatgtt acactgaatg taaacggtgg
     4561 ctttctaaat atgggaaatg tagaattaat ctacaataaa ggaactttaa atacagctaa
     4621 tggtaatgca aaactaaatg ttggcggcaa gtacctgcaa acgcatggtg acttaagagg
     4681 tgtttttaat ttgtcttcaa ctgcagcagg aaatacagag tttaaattta attctcttga
     4741 aatgacaggt gggttgttgt ttgcttatta tgcttgtaat gcaactggtg cattaaattc
     4801 tgtcactgtt acaaatgata tggtacttaa cttttcaaat gcatcagata aattcagagt
     4861 aaatggactt acaacattag caggtcagta tagcaagtcg cgctcattgt taaaggttga
     4921 tggcaacctg attctttcag gaaatgctgc agcagaattt acttcaagtg gaagtgttgg
     4981 agcagaagaa aacagaatta aaggggatgt taatatatca gggcttaaca gtaatttcaa
     5041 catgggcagc catgctgtta ttttatctgt aaatggtgat gttaacattg atggtggaaa
     5101 cacctgtttt tcaaaaacac ctggagtttc acaaattagt ttaaataaga atttctcatt
     5161 acaatcaggc acttttatct taaagggtca tgaaggcaag tcagaactaa ctgtgaacgg
     5221 tgcatattcg cagaccggtg gtgtatttta tttgcactcg aatacattaa ctgcatcaaa
     5281 tgattctgta aaagcttact ttaaaagttc attccgtcag tggaatggca ttataaactt
     5341 tgatgataat cctgaaggcg gtaagtctgt actttttatt caggggaatg aatttatagc
     5401 caaaggcagt tcattgataa caagagcagg aacaggcatt gggtctgttt ttggaaattt
     5461 gatatattgt acaaatggag acctaaatta ccagagaagc acagcgtctg taattgatca
     5521 gattaaacaa cgtattacag taaattgcag acttaaagta acagacggga atctactact
     5581 tagtagtcat agtcagcctg ctgttgactt tttaagagtt gaatcgggtg caatattaaa
     5641 tttactaagt acacaaatta agtcgaacct gaaatatcag aattcaggtt ttactgttga
     5701 tgcgggaggt ttggtaagaa cagctaattc aaaaggcttt tataacacgt tgggcactgc
     5761 cgcagtttgc agtaatggta acctgaatta ttttcttgac agaaatagta tagttgatta
     5821 tgtagggagt aaccaattaa taaccggttc aggctccggt gttattaata aagaacagtt
     5881 taagtatggt gttttaaggg tgagtggaaa tggcattctg gcaactaatg ttgaagttcg
     5941 caatcgtata gaattaggca gctcaacaat taagctcaat caaaaaactt tgattataga
     6001 gaatgaaaac actaatgcat tatcagcatc aggcggctcg ttgatttgtg cttcagaatc
     6061 agataaactt atttggaaaa atatgaaagg tggaagtagt tatacaattc ctttctcaag
     6121 tgatggtaaa gttgtttcaa atgttgtagt gcaaccgatt gtttctggcg atatttcaat
     6181 gtataactat actggaaatc cggtttctga aaatttacct gatggcatac aaccgtttct
     6241 tgttgatggt aaggatgtaa ccgatgaatc tttagcaaga cgaattattg gtttcgattc
     6301 acaagtaaaa gcaaatatta ctttaaagtt ctttaagaat gaaattcctg gcttcaaaaa
     6361 tggtgataca ttagttctga taaaaaatat tggacaatgg gaagtgacac cattcagtaa
     6421 agtaggtgat gaacaaaatc cagtttctgt tcaggcaaca aatataaatg caaacgcagt
     6481 atttgcactt tgcaccggca taggagcgaa gatgcccgat aatactaatg gcaacaataa
     6541 ttctcaggga aattttagtg tagagcagat ttcaccggta ccattcaatg attttcttaa
     6601 agtaaacatt aatgctccaa caattggggt aatttatctg actattgtag gctctgatgg
     6661 gcgtgaactt agaaaacaaa tgtttgatgt ttctcctgga aagaacttgt tagcactaag
     6721 tgaactgagt agtttgccca caggtgtcta ttttatacag ataaatggat ttaagcaaac
     6781 cattaccaag aaagtaatgc ggcagtaatg aaatgtaaga aatcacaaat ttcgatttaa
     6841 gtttttacaa ccttttttaa tctctaagag tataatcaaa aggttcattc tataaaataa
     6901 ttattatggc aagaaaatta aaactccgtc agcgacaaaa gatgtctata atgtataagg
     6961 caatttttgt tactgccgga acatctataa tgggattggc agtatatctt actgtcgtta
     7021 tgaatacttc caatgtgaca gattcacgtg ctggagcact caccaatatg attcaggggt
     7081 atgatgtaaa taacggagaa gttatttgtg catttgattg gaatggtgac gatcctacaa
     7141 aggcaagtaa aggtcctgat gcaataaaag taagcccttt tgctgtagta atgtcagaag
     7201 gtgttgaagg cacaaacgga ttgagcgcag gtaaaggtaa agatggaatt gatttgacta
     7261 ttccaacaga taattatttt aatactgatg gaattgatat aagctttgat tataaaagac
     7321 tagatgataa ttgcgatttt ataagtcgtg gtcgttattt caactttgga atgaagaacg
     7381 gaaaattgat tattgcctat cgtgtaaatc tcgaaaaagg aagaagctat tctgttaatg
     7441 aaacaacaaa ttatgagttg gtaaaagata atgatttcag aaattaccgc tttgtctata
     7501 atccaaatac aggtaaaggt gaaattatgg ttgatgatgt agtggtttgg aattatcagg
     7561 ggccaaaaga gcgatcaatg ttctggaaaa attctgacaa tattgttatt gcaaagaata
     7621 tgaacggtgg cggtacagat aaaactattc ttgataattt tattattcgt agcacaacgc
     7681 atgtacaaca actgccaatc acattacttt ctttccaagc agaggctcgc gatcatcatg
     7741 taatggttag ttggtacacc tctcgcgaaa cagatataga ttcctttgtt gttgagcgct
     7801 ctgtagatgc tattgaatat caaaaaatag gagctataaa agctaatgga aattctgaag
     7861 tgttgcgtgc ttatgcattc gtagataaag ctcctttaga aaacaaaatt gcctattaca
     7921 ggttgatgcc ttcaaacaaa ccattaaaat caatagcggt gccgattata gggtatcgtt
     7981 ataggatgaa ccacccggaa acagcattta atccggctga ttatccagct gacacggtga
     8041 aataaaattt gatccgttta atagaagagg ctgtcagatt aatgacagcc tctttttatt
     8101 taggtgcttt tcgtaataag taaatggcaa taaatgcata tactaaattg gggatccaca
     8161 cagcgacaac tgccggcaga tttccatttg tagcaaatgt tgtgctcacc tgcataaaca
     8221 taatgtaagt gaaactcaat aatattcctg cccctaattg taaacctata ccgccacgta
     8281 cttttttact tgaaatggca acgccaataa gtgtaagaat gaaagttgca aaaggatagg
     8341 aggtgcgcct gtatttttct acttcgtaaa aaggaatcac ttcagagcct cttgattttt
     8401 cttctttaat atagctgttt aactccgaaa aatccattgc ctcaataaag ttcattcttc
     8461 tgttaaattc agatgggtgt attgataatg tagtatctag cctgatgcct ttctttaatt
     8521 cttctgtcat accattaata tgtctgatgg tataattttc aatgctccac ttttgcgtta
     8581 atgaatccca tgtaattcta tcagccatga gtttagttac taattttcct ccttcaaatt
     8641 tttcaatgct gaacataaag cctgtgttgg ctttgttatc gtagctttca aaataaacaa
     8701 actcaccggg ttttatttgt ctgtgaatat ttcttccgcg atagacataa ggatttttaa
     8761 tataaatatt ttcgaattga agccgtacaa ggtttgcatg tggaatcacc cagttgttta
     8821 aataaaaaga tgcaatagca attactgttg cagaaataat ataaggtcgg agaaagcggt
     8881 tgaaactgat gccactgttt aaaatggcaa taatttctgt ctgcccggcc attttagagg
     8941 tgaaaaatat tactgaaata aaaacaaata atggactgaa taaattggca aaatagggga
     9001 tgaagtttag gtaataatcg aatgcaatag cctttaatgg agcatgtctc tcaataaaat
     9061 catctacttt ttcagaaata tcaaacacaa cggcaataca aataattagt cctatggcaa
     9121 acaggaatgt gcctaaaaac ttttttataa tgtagaggtc taagattttt gccttcaatg
     9181 caaacttttt tgcgcaaaac taaattaaat cacttatctt taataacaag aacagggatg
     9241 aatatcgtta ataaaaagtt tgaagcttta aaaatacccc attatgacag cgtattttag
     9301 cgtcaaaaat cggaaatgag atatcatttt attgctattg caggaagtgc tatgcataat
     9361 ctggcacttg ccttacatca acttggtcat gaagtgtcgg gcagtgatga tgaaattgtt
     9421 gagcctgcaa agacaagatt ggctaaatcg ggaatattac ctgagaaaac aggttggttt
     9481 cctgaaaaaa ttaatgctga tattgatgcc gttatattgg gcatgcatgc tcgtgccgat
     9541 aaccctgaac ttgcagaggc tattcaaaaa aatattaaag tgttttctta tcctgaattt
     9601 ttgtatgaac attcaaaaaa taaaaccaga gttgtaatag gtggcagcca tggcaagaca
     9661 accattacat ccatgattct acatgtttta aaaacatcag gaaataactt tgattttatg
     9721 gtaggtgcac aacttgaagg ttttgatacc atggttaaac tatcaaacga tgcccctata
     9781 attgttctgg agggagatga atatttgtct tctccgattg acagacgacc taagtttcat
     9841 ctgtatcatg cacaaattgg tctcattagt ggcattgcat gggatcatat aaatgtgttt
     9901 ccaactctgg agatttatga ggagcaattc aggatttttg caagagaaat tccacgtgat
     9961 ggaaaattgt tttactgcat gaatgatgat gctgttcgta aaatttgtga ggctgaaaat
    10021 gttgtagctg atagaaaagg ttattccatt ccgaaacatg ttattagaaa tggaaaaaca
    10081 tttatttacg atgactataa taaggaagtg ccgcttcaga tttttggtga gcataatctg
    10141 atgaatctag aaggggcaag gatgatatgc aactcaatag gtgtcaataa cgaagttttt
    10201 tataatgcaa taaaatcatt taaaggagca gccagacggc ttgaattact tgaagagaat
    10261 gatcagtttg ccgtttttag agattttgca cattcacctt caaaacttaa agcaacatta
    10321 gaagcggtac gcaggcaatt cccggaacga aaacttattg cctgtatgga gttacatacg
    10381 tttagcagtt taaatgaaaa ttttcttgat gaatataaag gcacaatggc tgatgcagat
    10441 gttgcagtag tttattttaa tccaaaaact attgaacata aaaaattgcc tcccattagt
    10501 tccgaaaaaa ttttccaatc atttgacaga agcgacataa aagttattaa tgactcaatg
    10561 caattaataa attttctgaa cacagaagat aaacaacaag tagtttattt aatgatgagc
    10621 tcaggtactt ttgacggatt aaatctaaaa tcttttgctg aaagcttaaa cacctcacat
    10681 caaaaagtat aatcaataaa tatcaacttt tcttttgttg ccaaatgccc aacacaaaaa
    10741 taagggtagg gctgcagacc atgttatctg attatgctgc ccttcgggaa tttctacata
    10801 ttgaatattt ttttcaggaa cacctttttg tttcatttca aaaatcagat cacgggcatc
    10861 atcaatagag tcaataacac catttttatt cctgtctgca gtttcatctt tagtgcctgt
    10921 ttgaatccag cattttattg ccggtgccga tttacttttc tttaccacct gatgcataat
    10981 tctgtcatca tcagtatatc cgtcttcgta agattttttt ctccaccaga aactgccgct
    11041 aaaaacacct acagtttcaa aaacagaagg atggttccat gccaaatcaa aagctgttaa
    11101 tccacccaat gaaaaaccgg ctattgcagt tttggatggt gtaaatttta ttcctgtaaa
    11161 actgctgcaa tgcgcaatca gttcgtcaag aataaatttc gaaaattgtt ttgctttatt
    11221 tccacgcttt agatagtcag ccttacctac aacaccatat tcctgcaatc tgtttttatt
    11281 tgcatgaata gcaattacta tgataggttt aatttttttt aactgtgtca aactctgcag
    11341 tgtatctttt aggtgtaaca attctgcatc ctgaccatca agcataaaca atactccggt
    11401 gtttttagag tattgtgcag gcaaataata atcaattcgt atttcgcgct ttaaaaactt
    11461 acttggaaat atggttgata ttaaatcata cttccactct gacttaggtg atgtcataac
    11521 ttaataaatg caatatcaga gccaacccga ctttttgcgt cttgttgcac ctaagcctaa
    11581 tgctccaagt aagcttcttg ttagtacact tgttacagtt ctcatcactt gctttcctgt
    11641 agaactgttt agcacctgat ccaactggct gggttcttct tttgcttcag ctgcttttgt
    11701 tgcttttgta ttttcagcaa cctgcgtttc taatttttta gagagtactt caaatgcact
    11761 ttctctgtca agtgtttcat tgtattttgt tgtcagacga gatttagaaa tactttttgt
    11821 caattcatcc ggagttaaaa tgtccattcg tgatgccggt gtacaaagca aagtatgagc
    11881 tattggtgtt ggcacacctt tttcatttaa aactgtgatt gctgcttcac caatacctaa
    11941 agtagtaagt aattcatcaa ctttatagta gtctgatagc ggataatttt ctgctataag
    12001 ttttaatgct tttctgtcgt ttgctgtaaa tactctgaaa gcatgttgta cttttaatcc
    12061 taactgcgcc aatacggcag ccggtatatc ttccggtgat tgtgtaacaa aaaatactcc
    12121 aatgcctttt gatctaatca attttattac tgtttcaatc tgatctaaca acactttaga
    12181 tgcattgtta aaaattaaat gcgcttcgtc aataaatatt accaatttag gtgcatccgg
    12241 gtcacccact tcaggaaatg ttgaatatac ttcagccaat aaactcaaca taaaagttga
    12301 aaataattta ggcttatcct gaatatcggt caacctgatg atattcaatt tgccattgcc
    12361 attttcgtct attcctaaca agtcttcgac ttcaaaagat ttttcaccga agaatttctc
    12421 tgcaccttgc tgttctatct ctattacttt acgaatgatg gttgatgccg aagcactact
    12481 tatagcacca taatctgctt taatttcatc cttaccttca ttagttaaaa actgtaatac
    12541 ctttttgaaa tctttcaaat caagtaaagg catttgttta tcatcgcaat atttaaatat
    12601 cagcgaaaca acaccttgtt gtgtgtcatt caaatcaagg attttagaaa aaagtacagg
    12661 cccaaattca gacacagtag ctcttaatcg cacacctttt tcatctgata aagtcagaaa
    12721 ctcaaccgga aattgcagtg gcttccaggt aagtcctaat ttctgatagc gttcatcaat
    12781 ttttggattt gttgttcccg gacttgccaa tccacttaaa tcgcctttta catccatcaa
    12841 taaacatgaa acacctttcg cagaaagcga ttctgctatg ccctgcatgg ttttggtttt
    12901 tcccgttcct gtagctcctg caattaaacc atgacggtta aaagttttta atggtgcttt
    12961 tatgaaactt cctgtcagcg tttcgccttt aagtaccggt gcaccgagta cgatgctgtc
    13021 gcctttaaat tgatattctt tatttacgac ttctttaaac tgttctgtta agctcattta
    13081 tgtttagtta aaaaactgct gacaaatgta tgcctaaaag atgaaattaa acagaaaggt
    13141 ttatttcttt gcaacttgtt aatgtgtttg acttaatgcc acaaaatatg cctgagggtg
    13201 tttcagcttt tcaaagaatt tataaacagc tatttctgat tttaaaatcg gttataacgt
    13261 tttcctatct caaaataata aagaacaagt ttttaagaag tgcagctatt ttgtttatgc
    13321 tgatggttta ttatgttgtg ctactgcata taaactttct ttggctattt ggctatatgc
    13381 caaaaatgag tgaagtcaga aatccgcaag tggcaattgc caccgaaatt tatacagaag
    13441 atagtgtact tattggaaaa tattatcatg aaaacaggac acctgtaagt tttgattcaa
    13501 tttcgactta tgcagtacag gcacttgttg ccacagaaga tgtaaggttc tataaacact
    13561 tcggccttga cctttatgca ttaatcagtg gtgcatattc cactgccaaa ggagatgagc
    13621 gtggtgctag cacaataaca caacagctag ccaaaaatat gtacagta
//
LOCUS       DAOOOL010000073        13125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_431_length_13125_cov_88.150421, whole genome shotgun sequence.
ACCESSION   DAOOOL010000073 DAOOOL010000000
VERSION     DAOOOL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_13125_cov_88.150421"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PKU82_07295"
     CDS             <1..996
                     /locus_tag="PKU82_07295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ82713.1"
                     /translation="FLPYGASTRNAGFACIGSTSEILNDLEKESEDKVFQRVEKRYKG
                     LLGLRELLGDKNIEYEHNFGFEIFTETDSQSFRECESKLEYLNTQLKSITRDNTYTIK
                     NEHIANFGLSNVENLIQNKFEGQLDTGKMMFVLSEKVRQSGVNFLNGLEVKQIYNESS
                     GVILMTSEGFKIKTGKVIICTNGFVKEFLSDLSITPGRAQVMITNEINNLKLCGSFHY
                     QSGFYYFRNVGRRVLIGGGRNLDFQGETTTSFGVTEQIQSAIEKLLKEIILQGVEYKI
                     EQRWSGIMGLGESKSPIIQEVKPNIYCAVRLGGMGVALGYLVGKEVADLVNLNYQ"
     gene            1097..2812
                     /locus_tag="PKU82_07300"
     CDS             1097..2812
                     /locus_tag="PKU82_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOZ82714.1"
                     /translation="MSIIDWLVLALTLLAIVFYGIYKSRHKKTLDSYLIANRELPWYN
                     VCLSVMATQASAITFLSAPGQAYSDGMRFVQFYFGLPLAMIFLSATFIPAFRKFNIYT
                     AYEFLERRFDHKTRKLTALLFLLQRGISTGISIYAPSIVLSTVLNIPIFYTTMIIGLL
                     VIIYTFYGGTLAVSHSQVLQMTIIFSSIIIAGVIVLSLIGTDASLYESLKIAGIHNRM
                     NIIDFKFDWNNRYNVWSGIIGGFFLQLSYFGTDQSQVGRYLTGKDSSASKLGLIVNGM
                     VKIPMQFVILFIGILVFSFYQFQPPPIFFNENIEKIAKESPMADNYNSLEHKFNDINQ
                     SNSHLSKDYLKYLRSNDLRNAEITKAKILQTSAEANEVKQEAVKIIKTINPGADINDT
                     NFVFLHFVMHHFPKGFIGLIIAIIFLASMGSLAAGINSLTSTSVVDFYFRSSKKEHSD
                     SKKLYYSKIATLLWGIFCILCAFFAGNTGNLIEAVNILGSLFYGTILGIFISAIFVKH
                     LDGNSVFIAALITEAIVIAFWYFDVMAFLWLNLAGCLLVVLIALAIQFCTQNLNRMFN
                     KTISK"
     gene            2858..3175
                     /locus_tag="PKU82_07305"
     CDS             2858..3175
                     /locus_tag="PKU82_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ82715.1"
                     /translation="MQEITVHELKQWLDEGKNFQLIDIREEYEYDACNLNGTLIPMGE
                     IIDRTEEIAKDKPVVIHCRSGSRSAAIYQVLSTQYGFTNLFNLKGGIKAYSQEIDPTL
                     NVI"
     gene            3185..3829
                     /locus_tag="PKU82_07310"
     CDS             3185..3829
                     /locus_tag="PKU82_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOZ82716.1"
                     /translation="MEHLKFLIDFILHIDKHLFELVQRYDTWIYLILFLIVFAETGLV
                     VTPLLPGDSLLFAAGALAAPPDNPLNVFYLMIIFFFAAFGGDNTNYFIGNYIGPKVFE
                     KNYRFLKKDYLNKTQEFYEKHGGKAVIFARFVPIVRTFAPFVAGVGSMTYNRFIGFSV
                     LASILWINICVWAGYFFGRLEIVKNNFSLVVIAIIGISLVPILIGIIKQKFSSK"
     gene            3826..4548
                     /locus_tag="PKU82_07315"
     CDS             3826..4548
                     /locus_tag="PKU82_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOZ82717.1"
                     /translation="MRNFGLIGFPLSHSFSQKFFTDKFKKLNLKCVYNNYELSNLNNL
                     RELISTNQLTGLNVTSPYKTGVIPYLDRLEETAEAIGSINTILIKDKKLIGYNTDIIG
                     FKISLERLIGNQKPPTLILGTGGSAKTVAFVLKQKNIPFQFVSRKKKEGAISYSQSDD
                     FLSQNILIINCTPIGRFPDFGKKPPINYTKLTDKHFLFDLNYNPEITAFMTEGINKGC
                     KVCNGYDMLIEQAEASWVIWNT"
     gene            4665..5213
                     /locus_tag="PKU82_07320"
     CDS             4665..5213
                     /locus_tag="PKU82_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82718.1"
                     /translation="MNNILKDNKTKFITLSILVVAISRLLPHPYNFTPIGAIALFGGT
                     FLKDKKMAFGLPLAALLVSDLLLQLIKPGSAFYEGWFWVYLSFILITPLGFVLRNKLK
                     VQNVVAASLMGSIIFFVLTNFGVWATGLMYPLTGAGLMSCYVSAVPFFGGTIMGDLFF
                     NALLFGVFAFAKWRMPVLVKEA"
     gene            5248..6609
                     /locus_tag="PKU82_07325"
     CDS             5248..6609
                     /locus_tag="PKU82_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOZ82719.1"
                     /translation="MYFNNILETIGNTPLVKLNSVTQGVQATVLAKIETVNPGNSIKD
                     RMALKMIEDAEKSGKLKPGGTIIEGTSGNTGMGLAIAAIIKGYKCIFTTTDKQSKEKV
                     DALRAFGAEVIVCPTNVEPEDPRSYYSVAARLSKEIPNAFYPNQYDNLSNRQAHYEQT
                     GPEIWEQTEGKITHLVVGAGTGGTITGTGRYLKEKNPKIKVWAIDTYGSALKKYHETG
                     ILDKNEIYPYVTEGIGEDFIPQNYDFTVIDHFEKVTDKDAALMTRDLVRKEGIWVGNS
                     AGSAVAGLLQMKEQLKPTDVVVIIFHDHGSRYIAKIFNDDWMRSKGYFDKKGMTASDI
                     IGSKKDVNLTTVEATLPIGDTLKIIKTNNFSQIPITREGRIVGNITENHIYSSLLKSP
                     ELKNKPVSDIMQEAMPFIDINTSIDTLSDLLSKNNQAVIVKDFKADRNYIVTRQDVVD
                     ALA"
     gene            6629..7402
                     /locus_tag="PKU82_07330"
     CDS             6629..7402
                     /locus_tag="PKU82_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOZ82720.1"
                     /translation="MSFGICEMSLVPMRREPSHRSELVSQILFGEHYEVVESQKEWFK
                     IRTSHDGYEGFIALNQHTEISYNDFQFLQKNGAFVCFDLVQILLHDNKIQTVILGSAL
                     PFFENQHCRINTRTYRFDGNAKFPQKAATSALLIENAYMYLNAPYLWGGRSPFGIDCS
                     GFTQLVYKLSGYSLLRDAALQAEQGNIVNLVDEAMPGDLAFFDNEEGNIIHVGILTAR
                     DTIIHASGKVRIDAFDQVGIYNQETKRYTHKLRLIKRIF"
     gene            7411..7665
                     /locus_tag="PKU82_07335"
     CDS             7411..7665
                     /locus_tag="PKU82_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82721.1"
                     /translation="MNNPQLKASENFPATVNELYDEEGELVLILYTHELVDLPEGLWL
                     STEGYYEAVIKKTEIQIEDALFCISELNSISGMQYELTLS"
     gene            complement(7662..8732)
                     /locus_tag="PKU82_07340"
     CDS             complement(7662..8732)
                     /locus_tag="PKU82_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82722.1"
                     /translation="MSKTVIVAPLNWGLGHATRSKTVIDNLLAAGCKVIIASDGNSLS
                     VLKRQFPVLQAEELPSYSPVYPLNGNMVWAMLKQTPKMLLNISNEQSAIKKIASKYNA
                     EIIISDNRYGCFTNFTKNIFLSHQLMIQSPESLLFLRPSINRLHAKYINRFHECWIPD
                     VEHGFKLSGILSENKFIKIPKYNCGLLSSFQSSNLSNDKKYDIVAILSGPEPQRTIFE
                     GKIIEQLEKLHCSALLIRGKISDPNEIPDRGALQFKNYMPADEIAKFINTDTLVICRS
                     GYSTIMDIAQLGVRALLVPTPGQTEQEYLASYLEENKIFMMQTQSNIDIQNAIEKKLE
                     YAAIRIEKNILLQQNLNRILLS"
     gene            complement(8760..11048)
                     /locus_tag="PKU82_07345"
     CDS             complement(8760..11048)
                     /locus_tag="PKU82_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ82723.1"
                     /translation="MMQKWIKVLSIISLLNVNILFAQKVGLVLSGGGARGFAHIGVIK
                     ALEENGIPIDFITGTSAGALVGGMYVSGTSPQQMQNIVLSDDFKNWATGTLSDELIYF
                     FKERERDASWATVKLSYDSTLRMNLNGALVNSGQIDFALLENHSAASAGADYDFNNLF
                     VPFRCVASDIKNKRPVIFNCGDLSQAIRASIAYPLYLSPMTADNQLYYDGGIYNNFPS
                     DVMLNEFDPDIMIGVSVGGGPDIPKEDNMFSQLRTMIIQSQHVVLPGADDILIQPDVT
                     NIGVFDFYNFQALIDSGYKATMARMDDIKQAIARRISPEELTQKRDNFISKLHPLIVG
                     DIVVTGVSPEKTKYIKSILNPRGSNMLLRQLKPRYFKLLADENIRYAFPKLVYDPKTE
                     VYNLEIYIKPETDLRVDIGGNFSSRPISEGFIGFRYNILGPYSLSIHANTYFGKLYNS
                     GQLKLRLDIPGRPDFFIEPFTTLNRMDYFKSSSFFKDDIKPAYLLVTDQSYGLNIGMP
                     ARNKGALTVGGNYFNIKDRYYQTRSFSVNDTADFTRFEGLSCVVGFERNTLNRKMYAS
                     KGSYLAIRTRFVSGNEVTNPGSLQDNAPTIREWHQWWQVKMIYDNYFFRAGRYKLGFY
                     AETNFSGQPFFSNYTATILQMPGFYPTQESRTLFLDNFHAPNYLGIGVRNVFSLRSNI
                     DFRLEGYAFQPFQAVVKNDNEKTVFGDALSARYFIASFSGIFHSPVGPVAMTLNYYDR
                     KTKSFTVLFHIGYILFNKHALD"
     gene            complement(11061..11867)
                     /locus_tag="PKU82_07350"
     CDS             complement(11061..11867)
                     /locus_tag="PKU82_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOZ82724.1"
                     /translation="MLKFIDQTGFEIKLETPPQRIVSLVPSQTELLADLGLQDRIVGI
                     TKYCIYPPELFLSVPRVGGTKSVNHELINQLKPDIIIANKEENNREAIEQLRKNFPVW
                     LSDVSNLHEALDMIYNIGVMTDCQSRASEINSQIAEKFAALTIKNKKHTAKRALYFIW
                     RNPYMCVGGDTFISDMMNMAGYDNLMYYHKRYPELSEKKMAELNPEIILLSSEPYPFK
                     ARHIEEFKTICPKAEIKLVDGEFFAWYGSRMIKAADYFHQLCDSNSNVSE"
     gene            complement(11934..12410)
                     /locus_tag="PKU82_07355"
     CDS             complement(11934..12410)
                     /locus_tag="PKU82_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82725.1"
                     /translation="MRKLFMSSAICFAILLSTMVSFSQQTEKKSTESGFFVRNGLVYY
                     IKGNTVNFLESDQTLDNGATINQRGMIKYPDGKTAQLQQGEILTFNNVLVPKAVEEHF
                     ELKDGKLQIVTNGIFTPVQKAMKLETGVTIDEDGHTSSGITLMPKQKMNMMGELIK"
     gene            complement(12466..12762)
                     /locus_tag="PKU82_07360"
     CDS             complement(12466..12762)
                     /locus_tag="PKU82_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82726.1"
                     /translation="MKKLVKRLSALGAMLIISTVAFSQSTTTQETEQTTPPPTETTES
                     TEDSTAATEAVPDSTAVAPEGDPAAGAANEKPATRHKLYSGKQRGNNVIYDPKK"
BASE COUNT         4313 a         2160 c         2306 g         4346 t
ORIGIN      
        1 ttcttgccct atggtgccag cacacgaaat gccggatttg cttgcatagg cagtacttct
       61 gaaatattaa atgatttaga aaaagagtca gaagacaaag tttttcaacg tgttgaaaaa
      121 agatacaagg gtttgcttgg attaagagaa ctattaggtg ataaaaatat tgaatatgaa
      181 cacaacttcg gatttgaaat ttttacggag actgatagcc aatcattccg ggaatgcgaa
      241 tcaaaattag aatacttaaa tacacaactt aaatctataa caagagacaa cacctataca
      301 ataaaaaatg aacatattgc aaattttggt ttaagcaatg tagaaaactt aattcagaac
      361 aaatttgaag ggcaattaga taccggcaag atgatgtttg ttttatctga aaaagtcagg
      421 caatcaggag taaatttttt gaatggactt gaagttaaac aaatttataa tgaaagttct
      481 ggtgttatct tgatgacttc agaagggttt aaaataaaaa ccggaaaagt tattatttgc
      541 acaaatggat ttgtaaaaga attcttatca gatttatcca tcactcccgg tagagcacag
      601 gtcatgataa caaatgaaat taacaattta aaattatgtg gtagttttca ttatcaaagt
      661 ggtttttatt attttagaaa tgtgggaaga agagtactta ttggcggggg cagaaattta
      721 gatttccaag gtgaaacaac tactagtttt ggtgtgacag aacaaattca atcagcaata
      781 gaaaaactat taaaagaaat aattttacaa ggtgttgaat ataaaattga acagcgctgg
      841 agtggcataa tgggtttggg tgaaagtaag tcaccaatta tccaagaagt taaacccaac
      901 atttattgtg cagtaagatt aggtggaatg ggtgtggcat taggatattt agtaggtaaa
      961 gaagtagcag atttagtgaa tctaaattac caatagttat gaagaaagaa attcaatgga
     1021 agcgccttta tttaattgtt gtagcactat tgattattca gattgcttta tactacataa
     1081 taactaggac attttcatga gtataataga ttggctggtt ttggcgctaa ctcttctggc
     1141 aattgttttc tatggtattt ataagagtcg tcataagaaa acattagata gttatttaat
     1201 tgccaacaga gaattacctt ggtacaatgt ctgtttaagt gtgatggcaa cacaagccag
     1261 tgccataaca tttttaagtg cacctgggca agcatacagt gatggaatgc gttttgttca
     1321 gttctatttc gggcttcctc ttgccatgat ttttctgagt gcgacattca tacctgcatt
     1381 cagaaaattt aatatttata cagcatatga atttttagag agacgttttg atcataagac
     1441 aaggaaatta acagcactac tttttttatt gcagagaggg atttctacag gaatttctat
     1501 ttatgctcct tcaattgtat tatctacggt acttaatatt ccaatttttt ataccacaat
     1561 gataattgga ctattagtta taatttacac tttttatgga gggactcttg ctgtatcaca
     1621 ttctcaggtg ttgcagatga caattatttt ttcaagtatt attatcgcag gtgtcattgt
     1681 tctttcttta attggaacag atgcttcact gtatgaaagt ttgaaaattg ccggaataca
     1741 caacagaatg aatattatag atttcaaatt tgattggaac aaccgataca atgtttggag
     1801 cggcatcatt ggtggctttt ttctacaact atcctatttt ggcacagacc aatcgcaggt
     1861 tgggcgttat ttaacgggca aagattcatc agctagtaag ctaggtttaa tagtaaatgg
     1921 gatggttaaa atacctatgc aattcgtgat tttatttatt ggcattcttg tgttttcatt
     1981 ctatcagttc caacctcctc ccattttctt taatgaaaat atagaaaaga ttgccaaaga
     2041 aagtccgatg gctgataact acaatagttt agaacataaa ttcaatgata ttaaccaaag
     2101 caattctcat ctatcaaaag actatttaaa atatttacgc agcaatgatc ttagaaatgc
     2161 tgaaatcact aaagccaaga tacttcaaac ttcggctgaa gccaatgaag taaagcaaga
     2221 agctgtaaaa ataatcaaga ctattaatcc aggagctgat ataaatgata ccaattttgt
     2281 ttttctgcat tttgtcatgc accattttcc taaagggttt ataggattga ttatagctat
     2341 tatttttctt gcatcaatgg ggtctttagc agcaggtatt aattcactta cttcaacatc
     2401 ggtagtagat ttctatttca ggtcatcaaa aaaagagcat agcgacagta agaaacttta
     2461 ctattctaaa attgcaacat tactttgggg aattttttgc atactctgtg cgttctttgc
     2521 cggcaataca ggaaatctga ttgaagcagt aaatatattg gggtcattat tctacggcac
     2581 tatattgggc attttcattt ctgcaatttt tgtaaaacac cttgatggca attccgtttt
     2641 tattgcagct ttaattactg aagctattgt aattgcattc tggtattttg atgtgatggc
     2701 atttttatgg ctaaatttag ccggatgcct tttggtagtt ttaattgctc tggcaataca
     2761 attctgcaca cagaatttga acagaatgtt caacaaaaca atatctaagt aaagtcaatt
     2821 cgtatctttg gcacaataat tttctcagat aaaaatcatg caagaaataa cagttcatga
     2881 attaaagcaa tggctagatg aaggtaaaaa ctttcagttg attgatattc gtgaagaata
     2941 tgaatacgat gcttgtaatt taaacggaac gttgatacca atgggagaaa tcatagacag
     3001 aactgaagaa attgcaaaag acaagcctgt tgttatacat tgcaggtcgg ggtcaaggag
     3061 tgcagcaatt tatcaagtac ttagtacaca atatgggttt acaaaccttt ttaatttaaa
     3121 aggtggtata aaagcctatt cacaagaaat tgatccgaca cttaacgtaa tataaattaa
     3181 aactgtggaa cacttaaaat ttctaattga ttttatttta catattgata aacatttgtt
     3241 cgaacttgta caacggtatg atacatggat atatcttatc ttatttctaa ttgtatttgc
     3301 tgaaacagga ttggttgtaa ctccactttt gccaggtgat tcacttttgt ttgcagcagg
     3361 agctttagct gccccacctg ataatccgtt aaacgttttt tatctgatga taattttctt
     3421 ttttgctgct tttggtggag acaacaccaa ctattttatc ggaaattata taggtccaaa
     3481 agtttttgaa aagaactatc gttttctcaa aaaagattat ttaaacaaaa cacaagaatt
     3541 ttatgaaaag catggtggta aagcagtaat atttgcacgt tttgttccaa tagtgcgaac
     3601 atttgctcca tttgtagccg gtgttggtag tatgacttat aatcgtttta ttggtttcag
     3661 tgttttagcg agtatcttat ggattaatat ttgcgtttgg gccggttatt ttttcggcag
     3721 attagagatt gtgaaaaaca acttttcatt agttgttatt gctataatcg gaatttcatt
     3781 agtgcccatt ttaataggaa taatcaagca gaaattctct tctaaatgag gaattttggt
     3841 ttaattggtt ttccactaag tcattccttt tcacaaaaat tttttactga taaatttaag
     3901 aagctaaact taaaatgtgt ttacaataat tacgaattaa gtaacctcaa caatttaagg
     3961 gaattaatca gcacaaatca gctaactggc ttaaatgtta cttcgccata taaaacaggt
     4021 gtaataccct atcttgaccg attggaagaa acagcagaag ctattggttc aatcaataca
     4081 atattgataa aggataaaaa actaattgga tacaatactg acataattgg ttttaaaata
     4141 agtcttgagc gacttattgg gaatcaaaaa cctccaacat taatattagg aacaggaggt
     4201 tctgcgaaaa ccgttgcttt tgttctaaag caaaaaaata ttccatttca attcgtttcc
     4261 agaaagaaaa aagaaggtgc aatttcttat agtcaatcag atgacttttt aagtcaaaat
     4321 attttgataa ttaattgtac accaataggg aggtttcctg attttggcaa aaaacctcca
     4381 ataaattaca ccaagttaac cgacaagcac tttctatttg acttaaatta caatcctgaa
     4441 attacagcat ttatgactga gggtataaac aaagggtgca aggtttgtaa tggttatgac
     4501 atgcttattg agcaagctga ggcctcttgg gttatctgga atacttagtt atgaatactt
     4561 aaaagcctgt ttagcaccat taatcctagt taaaaaagca ttgaataaac ttttgaatct
     4621 ttcctgtcat aagcctatct ttgccattaa aatatcagta aattatgaat aacattttaa
     4681 aagacaacaa aacaaaattt ataacattat caattctggt agttgccatt tcacgccttt
     4741 taccacatcc ttataatttc actcctattg gtgctattgc actttttggg ggtacttttc
     4801 ttaaagataa gaagatggct tttggtcttc ctttagcagc attgttggtt tccgatcttt
     4861 tattacaact tataaagcca ggcagtgcat tttacgaagg atggttttgg gtgtacttaa
     4921 gttttattct aataacacca ttaggttttg tactcagaaa caaattaaaa gtacagaatg
     4981 tagttgcagc atctttaatg ggatcaataa tattctttgt actgaccaac tttggtgtat
     5041 gggcaacagg gttaatgtat ccgcttaccg gtgcaggtct aatgagttgc tatgttagcg
     5101 cagttccatt ttttggaggc actataatgg gtgatttatt tttcaatgca ctactttttg
     5161 gtgtatttgc atttgcaaaa tggagaatgc ctgtacttgt aaaagaagct tgatagaaaa
     5221 ttttataatc aattcaaata acatttaatg tatttcaaca atatacttga aaccattggt
     5281 aacacgccat tggtaaaatt gaattctgtg acacaaggag ttcaggctac agttttggct
     5341 aaaatcgaaa ctgtaaatcc aggcaactcg ataaaagaca gaatggcact gaagatgata
     5401 gaagatgccg aaaagagtgg aaagttaaaa cccggtggaa caatcataga aggaactagt
     5461 ggtaacacag gaatgggatt ggctattgct gcaatcatta aagggtacaa atgtattttt
     5521 acaactactg ataaacaaag caaagaaaaa gtagatgcat tgcgtgcttt tggtgcagaa
     5581 gtaattgttt gcccaactaa tgttgaacca gaagaccctc gctcctacta ttctgttgca
     5641 gccagactca gtaaagaaat tccaaatgca ttttatccaa atcagtatga caatttaagt
     5701 aatcgtcagg cacattatga gcaaaccggt ccagaaattt gggaacagac agaagggaaa
     5761 atcacacact tggtagttgg tgctggtaca ggcggaacga taacaggcac tggtcgttat
     5821 ttaaaagaaa aaaatccaaa aattaaagta tgggcaattg acacttatgg ttcagcgctt
     5881 aaaaaatatc atgaaacagg tattttagat aagaatgaaa tttaccctta tgtaacagag
     5941 ggtataggag aagattttat accacagaat tacgatttta ctgtaataga tcattttgaa
     6001 aaagttactg ataaggatgc ggcattgatg acacgtgatt tggttcgcaa agaaggtatt
     6061 tgggttggta acagtgccgg ctcggctgtt gcagggttgc ttcagatgaa agagcaatta
     6121 aaaccaacag atgtcgttgt aattattttt cacgatcatg gatcgaggta cattgcaaaa
     6181 atattcaatg atgactggat gcgatctaaa ggctatttcg ataagaaagg catgacggca
     6241 tcagatatta tcggctcaaa aaaagacgtt aatttaacta ctgttgaagc aactttaccg
     6301 attggcgata cattgaaaat tataaaaaca aataattttt ctcaaatacc tatcacccgt
     6361 gaaggaagaa ttgttggtaa tattaccgag aatcatattt actcgtcact tttaaaaagt
     6421 cctgaactaa aaaataagcc ggttagtgat attatgcaag aagctatgcc atttattgat
     6481 atcaatactt caatagatac attaagtgac ttgcttagta aaaacaatca ggcagttatt
     6541 gtaaaagatt ttaaggctga cagaaactac attgtaacaa ggcaggatgt agttgatgct
     6601 ttagcgtaaa ttcaatattt agcagaatat gtcatttggc atttgtgaaa tgagtcttgt
     6661 tcctatgagg agggagcctt cgcacagaag tgagttggtt tcacaaatac tttttggtga
     6721 acattacgaa gtagttgaat cacaaaaaga gtggtttaaa atcagaactt cgcatgatgg
     6781 ctatgagggc ttcattgctt taaaccaaca tacagaaata agttataacg attttcaatt
     6841 tctacaaaaa aacggagcat ttgtttgctt tgacctggtg caaattttat tacacgacaa
     6901 taaaattcag actgtgattt taggtagtgc tttaccattt tttgaaaatc agcattgcag
     6961 aattaatacc agaacttacc ggtttgatgg caatgcaaag tttcctcaaa aagcagctac
     7021 atcagcatta ttaattgaaa atgcatacat gtatctcaat gcgccatatt tatggggagg
     7081 aagatctcct tttggtatag attgttccgg atttacgcag ttggtttata aattatcggg
     7141 atattctctt ttgcgcgatg ctgctttaca agctgaacaa ggaaatattg tgaaccttgt
     7201 tgatgaagcc atgccgggcg atttagcatt ttttgataat gaagaaggga atattattca
     7261 cgttggtatt ttaactgcac gagataccat tattcatgca tctgggaaag ttcgtattga
     7321 tgcatttgat caggtaggta tttacaatca ggaaaccaaa cgatacacac ataaactcag
     7381 gttgataaaa agaatatttt aaattcatcc atgaataatc cgcagctcaa agcttctgaa
     7441 aattttcctg caacagtaaa tgaattatat gatgaagagg gtgaattggt attgatttta
     7501 tatactcatg aactggttga tttgcccgaa ggcctatggc tatcaacaga aggatattat
     7561 gaagcagtta ttaaaaaaac tgaaatccaa attgaagatg ccttgttttg cattagtgag
     7621 ctcaactcca tatcaggaat gcaatatgag cttactttaa gttaggagag taatatccga
     7681 ttcaaatttt gttgtaataa aatattcttc tcaattctta ttgctgcgta ttccaacttc
     7741 ttttcaattg cattttgaat atcaatatta ctctgagttt gcatcatgaa aattttattc
     7801 tcttcaagat atgaagcaag gtattcctgt tctgtctgac ccggtgtagg taccaataat
     7861 gctcttactc ctaattgtgc aatatccata attgtggaat agcctgaccg acaaatcacc
     7921 aacgtatctg tatttatgaa cttagcaatt tcatctgctg gcatataatt tttaaattgt
     7981 aatgctcccc tatcaggaat ttcatttggg tcggaaattt tcccacgtat taataatgca
     8041 ctacaatgga gtttttctaa ctgttcaatt attttacctt caaatattgt tcgctgaggc
     8101 tccggacctg atagaattgc aactatatca tacttcttat catttgacag atttgaagat
     8161 tgaaatgaag aaagcaatcc acaattatac tttggaattt taataaactt attttcagat
     8221 aaaattccgg acagtttaaa tccatgttcc acatcaggaa tccaacattc atgaaaacga
     8281 tttatatatt ttgcatgtaa tctgttaatg gaaggccgca aaaacaacaa agattccggt
     8341 gattgaatca ttagttgatg tgataaaaaa atattctttg taaaatttgt aaaacaacca
     8401 tacctgttat cagaaataat aatttctgca ttatattttg aagcaatttt ctttatagcg
     8461 gattgttcat tgctaatatt taacagcatt tttggtgttt gtttaagcat tgcccatacc
     8521 atattaccat taagagggta aacaggacta tatgatggta attcttctgc ttgcagcaca
     8581 gggaattgcc gcttcaatac acttaagcta ttgccatcag atgcaataat cactttgcaa
     8641 ccggcagcta aaagattatc aattacagtt ttacttcttg ttgcgtgacc tagtccccaa
     8701 tttaagggag ctacaatgac tgttttactc ataaaatcaa tccgaaataa atcacttaac
     8761 taatccaatg catgtttatt aaacaatata tagccaatat ggaaaaggac agtaaatgat
     8821 tttgtttttc tgtcataata attaagtgtc attgctacag gacctaccgg actgtggaaa
     8881 attccggaaa atgatgcaat aaaatatctg gcagataatg catcaccaaa tactgttttc
     8941 tcattatcat ttttaacaac tgcctgaaat ggttgaaaag cataaccctc taaacggaaa
     9001 tcaatattcg aacgtaaact aaatacattt cgaactccaa tacctaaata gtttggtgca
     9061 tgaaaattgt cgagaaataa agttctactt tcctgagtgg gataaaatcc aggcatttgt
     9121 aaaatagtgg ctgtataatt actgaagaat ggttgacctg aaaaatttgt ttcagcataa
     9181 aacccgagtt tataccttcc tgctctgaaa aaataattat cgtaaatcat tttaacctgc
     9241 caccattgat gccactctct gatagttggg gcattatctt gtaaagaacc gggattggtt
     9301 acttcattgc cggaaacaaa acgtgttcga atagctaagt aagaaccttt tgatgcatac
     9361 atcttcctat ttaaagtgtt tctttcaaaa ccaacaacac aacttaatcc ctcaaacctg
     9421 gtaaagtctg cagtatcatt aacagagaaa cttctggttt gataatacct gtctttaatg
     9481 ttaaaataat ttccacctac agttagtgct cctttatttc gtgccggcat accaatattt
     9541 aatccatagg actggtctgt tactaataaa tatgcaggtt tgatatcatc tttaaaaaaa
     9601 ctactgcttt taaaatagtc catcctgttt aaagtagtaa acggctcaat aaaaaagtca
     9661 ggtctgccgg gtatatcgag cctcaattta agctgaccgg aattatatag ttttccgaaa
     9721 taggtattgg catggatact taaactatat ggaccaagaa tattatatcg gaagccaata
     9781 aacccttcac taattggtcg tgaagagaaa tttcctccta tatcaactcg caaatctgtt
     9841 tccggtttaa tataaatctc cagattatat acttctgttt taggatcata aaccaatttt
     9901 ggaaatgcat atcttatatt ttcatctgca agcaatttaa aataacgggg tttgagttgt
     9961 cgaagtagca tattgcttcc gcgaggattc aaaatagact taatatattt tgttttttcg
    10021 gggcttacac ctgttactac aatgtcgcca actattaaag gatgtaattt gctaataaag
    10081 ttatctcgtt tctgggttaa ttcttcgggt gagattcttc tggcaatagc ttgtttgata
    10141 tcatccattc gagccattgt tgctttataa ccactatcaa tcaaagcttg aaaattataa
    10201 aaatcaaata ctccaatatt ggtaacatca ggttgaatta aaatatcatc agctccgggc
    10261 aatacaacat gctgcgattg aattatcatt gttctgagtt gtgaaaacat attgtcttct
    10321 ttcggaatat cagggccacc gcccactgaa acaccaatca taatatcagg atcaaactca
    10381 ttgagcataa catctgaggg aaagttatta taaattccac catcgtagta aagctgatta
    10441 tcggctgtca taggtgagag atacagcgga taggcaattg aggctcttat tgcctgacta
    10501 aggtcgccac aattaaaaat taccgggcgt ttgtttttaa tatctgaagc aacacaacga
    10561 aatggaacaa agaggttatt aaaatcataa tcggcaccgg cacttgcagc tgaatgattt
    10621 tcaagcagtg caaagtctat ttgtccacta ttgaccaatg ctccattaag attcattctt
    10681 aaagttgaat catagctaag tttaacagtt gcccatgatg catctctttc gcgttccttg
    10741 aaaaaatata taagttcatc gctcaaggtg cctgttgccc aatttttaaa atcgtcactt
    10801 aaaacaatat tttgcatttg ttgtgggctg gtacctgaaa catacatgcc tccaacaaga
    10861 gctcctgctg aagttcctgt aataaaatca ataggaattc cattttcttc caatgctttt
    10921 atcacgccaa tatgggcaaa tcctcttgca ccgcctccac ttaataccaa accaactttt
    10981 tgcgcaaata gaatatttac atttaataaa ctaattatag ataacacctt tatccatttc
    11041 tgcatcatcg tgcaaaggta ttattctgac acatttgaat tactgtcgca tagttgatgg
    11101 aaataatcag cagcttttat catacggctg ccataccatg caaaaaattc accatcaacc
    11161 aattttatct cagctttagg acagatcgtt ttaaattctt caatatggcg tgctttaaaa
    11221 ggatatggct ctgaagaaag cagaataatt tcaggattaa gttctgccat tttcttttct
    11281 gaaagctcag gatatctttt gtgatagtac atcagattat catagccggc catgttcatc
    11341 atatcgctta taaatgtatc acctcccaca cacatatagg ggtttctcca aataaaatac
    11401 aatgctcttt ttgctgtatg ttttttattt ttgatggtaa gagcagcaaa tttttccgca
    11461 atctgactat tgatttctga agcgcgactc tgacaatcgg tcattacacc tatattataa
    11521 atcatgtcca atgcttcgtg aagattacta acatcactta accagaccgg aaaatttttt
    11581 cttagttgct caatagcttc gcgattgttt tcttctttat tagctataat aatatcgggc
    11641 ttcagttggt tgattaactc atgattcaca gattttgttc caccgacccg aggtacagat
    11701 aaaaaaagtt cgggtggata aatgcaatat tttgtaatac caactattct atcttgtagt
    11761 cctaaatctg caagcaactc tgtttgtgat gggactaagg aaacaattct ttgaggtggt
    11821 gtttccaatt taatttcaaa tcctgtttga tctataaact tcaacatgtc aggtaaagtt
    11881 aagcataaaa aaagccggta ttacccggct tttacatatt ttttaaacca acactatttt
    11941 attaattctc ccatcatgtt cattttttgt tttggcataa gggttattcc tgatgatgta
    12001 tgaccatctt catcaattgt gacaccggtt tccaatttca tggctttctg tacaggagta
    12061 aaaatgccat tagttacaat ttgaagctta ccatctttta attcaaaatg ttcttcaact
    12121 gccttcggaa caagaacgtt attgaatgtt aaaatctctc cctgttgtaa ctgcgctgtc
    12181 tttccatcgg gatattttat cattccacgt tgattaatcg ttgcaccatt atcaagtgtt
    12241 tgatcgctct ccagaaaatt tactgtgttg cctttaatat aatagacaag gccattgcga
    12301 acaaagaaac ccgattctgt tgattttttt tcagtctgtt gactgaaaga caccattgtg
    12361 cttagtaaaa ttgcaaagca aatagctgaa ctcatgaata actttctcat tgatttttat
    12421 tttataaaag taaaattacg atgaaataaa ttaaaaaaca accccttact ttttaggatc
    12481 gtaaattaca ttgttgccac gttgcttgcc agagtacaac ttgtgtctgg tagcaggttt
    12541 ttcattagct gcaccggcag caggatcacc ttcgggagca actgcagttg aatcgggtac
    12601 agcctcagta gctgctgtgg agtcttctgt gctttctgta gtttctgtag gtggtggtgt
    12661 agtttgttct gtttcctgtg tagtagttga ctgcgaaaat gctacagttg agataatcaa
    12721 cattgcacca agggcagaca agcgtttaac taactttttc attgtattat ttttttccaa
    12781 aattaataat caaaacgatg attcaacaat catattttat ttttattgca ttcagatgtt
    12841 aataaatcaa gacttcacaa tcaaaagcac tatttcattg gaatttggct ttttacttag
    12901 gataaataaa aaagggaaac aactattatg ttgttcccct ctaacctaaa aacaataaac
    12961 cctccacaaa tgtaggagaa taccgttttg aggatcagat tgttaataac tttttattta
    13021 cccattttct taacatctgc aatcaacttt tcattcattt taatgtaact gttatctcgt
    13081 ttctgggtta attcttcggg tgagattctt ctggcaatag cttgt
//
LOCUS       DAOOOL010000074        12748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_451_length_12748_cov_142.377295, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000074 DAOOOL010000000
VERSION     DAOOOL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_12748_cov_142.377295"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PKU82_07365"
     CDS             <1..1329
                     /locus_tag="PKU82_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOZ82727.1"
                     /translation="IYGKTSDLATNGDGRMYAATQLGYIYIINPGGVTDTIPFLDINS
                     KVTPTSLSNTGEQGLLGLAFSPNYTTDSSFYVYYTNKTGNGNSVLARYKRSADPNVAD
                     TVSAEILFALAQPYTNHNGGCLKFGNDGYLYLALGDGGDVGDPGNRAQNMNMYFGKIL
                     RIDVLQPGAYTIPSTNPFFGVMNVKNEIWASGFRNPWKFSFDRLTHDMWIGDVGQDIW
                     EEIDFQQANSTGGENYGWRCYEGLVTYNTSVGCIGNYISPVHVYSHASTGGCSVTGGF
                     VYRGTAYPALYGYYFFADFCNGTIYALDPNNGNAVTVAGTFPGRAFSTFGEDDNGELY
                     IADLVNQKVYQVTDTTTSISEYGNDVNNVDMSFNQNEQSLQMNLTSKSNNQATLYLYD
                     LKGTLITAWKLSLKSGNNTFKLPINLTSGIYLSNINSVNASLNIKIPVIK"
     gene            complement(1335..1805)
                     /locus_tag="PKU82_07370"
     CDS             complement(1335..1805)
                     /locus_tag="PKU82_07370"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HOZ82728.1"
                     /translation="MKIRIFNIALVTLMGMWQLSIAQNSRKMEYNKLTPQEEKVLLFK
                     VTDRPFTGDYYLKKDVGTYICRRCNAPLYRSADKFEAHCGWPSFDDEIKGSVKRIPDA
                     DGMRTEIVCSNCDGHLGHVFLGEGFTAKNTRHCVNTSSLKFIPAGTPLPEVIKK"
     gene            1931..3319
                     /gene="lpdA"
                     /locus_tag="PKU82_07375"
     CDS             1931..3319
                     /gene="lpdA"
                     /locus_tag="PKU82_07375"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOZ82729.1"
                     /translation="MNFDLIIIGSGPGGYVAAIRASQLGLKTAIIEKENLGGICLNWG
                     CIPTKALLKSASVFEYLNHASDYGITVEGGKADFNAVIKRSRGVADGMSKGVQFLMKK
                     NKIEVINGFGKVKPGKSVEVTATDGSKKTYTAAHIILATGARSRELPNLKQDGKNIIG
                     YREAMSLPEQPKSMVVVGSGAIGVEFAYFYNAMGTKVTVVEFMPNIVPVEDEEVSKQL
                     ERSLKKVGIEIMTNSSVESVTVSGGTCSVKIKSAKGESVITCEKVLSAVGITTNIENI
                     GLEECGIITDKGKVVVNDFYQTNIPGIYAIGDIVKGQALAHVASAEGIICVEKIAGHH
                     PQPLDYNNIPGCTYCTPEIASVGMTEKAAKDAGIGIKVGKFPFSASGKAKAGGQSDGF
                     VKLIFDEKYGELLGAHLIGANVTEMIAELVLARKLETTGHEIIKAVHPHPTMSEAIME
                     AAAAAYNEVIHL"
     gene            3468..6833
                     /locus_tag="PKU82_07380"
     CDS             3468..6833
                     /locus_tag="PKU82_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOZ82730.1"
                     /translation="MQESKIYTPKHKIRIVTAASLFDGHDAAINIMRRIIQSSGAEVI
                     HLGHDRSAQEVVECAIQEDANAIAMTSYQGGHMEYFKYMYDLLKERGCGHIKIFGGGG
                     GTILPEEISELQDYGIARIYHPDDGRAMGLQGMINDMLQKCDFATGVNLNGEIKELTK
                     KNSKAIARLISCAENNQSEFEKLYPEITSSLSNKTKNSPVLGITGTGGAGKSSIVDEL
                     VRRFLLEFKDKYIAIISIDPSKRKTGGALLGDRIRMNSIRNPRVYMRSLATRQSNLAL
                     SRYVQEALDIVKAAQFDLIILETSGIGQSDTEITEHSNVSLYVMTPEYGAATQLEKID
                     MLDFADLIALNKFDKRGALDALRDVKKQYKRNHKLFDIGDEKIPVFGTIASQFNDPGM
                     NQLYKSVMNTIAAKTGADLKSDYHTTEEMSEKVFIIPPHRTRYLSEISENNRHYDQWV
                     EEQCTLAQKLYSLYESMQIIKGAAAEELKKQYDILERSFDGNNKKLLEDFPAKTEEYS
                     KDYYEFKVRDKVLKIKTFDESLSHNKIPKVAFPKYKAWGDLLKWILQENVPGEFPFTA
                     GVFRFKREGEDPTRMFAGEGCPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGNDPDHR
                     PDIYGKIGNSGVSVCCLDDAKKLYSGFNLCDPATSVSMTINGPASIIAAYFMNAAIDQ
                     QCEIYIRQNGLEKEVEKKIADEFSKNKSSQPKYNYEIPEGNNSLGLMLLGITGDKVLP
                     KDVYDKIKTDTLKQVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDMQQYFIDKAIRN
                     FYSVSISGYHIAEAGANPISQLAFTLSNGFTYVEYYLSRGMDINSFAPNLSFFFSNGI
                     DPEYSVIGRVARRIWAKAMKLKYKANERSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQ
                     ALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGLAKNENPLQGSFII
                     EELTDLVEEAVLSEFDRITERGGVLGAMETMYQRGKIQEESLYYESLKHTGAYPIIGV
                     NTFLSSKGSPTILPKEVIRATEEEKQQQIATLKNLHARYASTAPEMLHDLQLTAIHNQ
                     NMFEKLMEVSKYCSLGQITNALFEVGGQYRRNM"
     gene            7172..7633
                     /gene="tnpA"
                     /locus_tag="PKU82_07385"
     CDS             7172..7633
                     /gene="tnpA"
                     /locus_tag="PKU82_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOZ82731.1"
                     /translation="MMSTYSQIYIQIVFAVKGRESLILPQWEERLHKYITGIVQNKEQ
                     KMLAINGMSDHLHFLIGMKPSCCLSDLVREIKKSSNDFIKVEKLSKFKFSWQEGYGAF
                     SYSHSQLNDVIAYITNQKEHHRKKSFKEEYVEFLKKFNVDYNEKYLFEWME"
     gene            7726..8499
                     /locus_tag="PKU82_07390"
                     /pseudo
     CDS             7726..8499
                     /locus_tag="PKU82_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745327.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(8557..9576)
                     /locus_tag="PKU82_07395"
     CDS             complement(8557..9576)
                     /locus_tag="PKU82_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOZ82732.1"
                     /translation="MKLVTYQNQNKTALGILLSDKVYNLSELSQRRGKTVLPDNMRDF
                     LTGEQLMMDAAMELNQWLSDGNADADGLSVANVNLLAPVPNPTSCRDGYAFRQHVEAA
                     RRNRRVEMIPEFDQYPIFYFTNHNAIQGPGDIFCMPDHFEQLDFELEAAIVIGRKGIN
                     VKAKDADSYIAGYMIMNDMSARKLQMEEMKLNLGPAKGKDFSTVIGPYLVTPDELASY
                     KTIAKSGHTGNNYNLKMTCKVNDIQVSEGNVGSMDWTFAEIIERCAYGVNILPGDVIG
                     SGTVGTGCFLELNGTGLFNNPDYKPQWLKEGDKVEMEIAGLGVLTNTIRKIDSDFSIL
                     ALKKM"
     gene            9723..10238
                     /locus_tag="PKU82_07400"
     CDS             9723..10238
                     /locus_tag="PKU82_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82733.1"
                     /translation="MIRKILATQALLLCSASFLFAQHTLVLKSGEKMTGTVQQIKDGN
                     ISFLFKGNPMNFKVSEASAIFFDAASETKADNSEMSADMRGVKYEMPGRTITKPPKIE
                     NLTMEKGVVVVEISIDKYGHVTKANPGAEGTTTTSSYLLTKAKQAAESVMFNNCPKCP
                     LDTKGTITLTF"
     gene            10265..11164
                     /gene="miaA"
                     /locus_tag="PKU82_07405"
     CDS             10265..11164
                     /gene="miaA"
                     /locus_tag="PKU82_07405"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOZ82734.1"
                     /translation="MPKDKKLIIIAGPTASGKTQIAIEVAKLHSTEIVSFDSRQIFRE
                     MTIGTAVPDAQQMNTVKHHFIHSHSIAEDMNASRYADEATNTINQLLEKNETVVLTGG
                     TGMYMHAALFGLDPTPNTDYQLRASLEKECAEKGLEYLAHQLKTLDEKSYHQIDIHNP
                     RRVIRALEIIYSSGKTRSSFIEDKNKISTYNFTLYVLNPDRQLLYDNINQRVDMMIEK
                     GLVDEAKLLLPYRHLTALHTVGYKELFEYFDRNITLDQAIEKIKQHTRNYAKRQLTWF
                     RQYDDAVWITPEEMKKKIEKKNL"
     gene            complement(11161..>12748)
                     /locus_tag="PKU82_07410"
     CDS             complement(11161..>12748)
                     /locus_tag="PKU82_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82735.1"
                     /translation="GIYNKGSNPVYTAEKLKVPVYTIAVGDTTINRDVLISDVAYNKV
                     AFLGNNFPVEVGVTMRQASGTAVTLTIKEDSLTLFSRMISASGNNYFAKIPVILSANR
                     KGIHKYKIRISEVDGEKNLLNNEADIYVDVSERKMKILLLAQSPHPDIAAIKSVLDKS
                     NLYETKVIFQEQADENFSNYNLVILHQLPSAKSTTLLQKIKTAEVPYFIIAGAQMNVA
                     AFNNSGTGVTINQPVAKMNEVYAKVSDQFGLFSLSDDMQKVLSQLPPLFVPFGNYKVE
                     KETNTLLAQRIGSIQSSMPLWYFSTSGTMRTGVLLGEGLWRWQLNEYALSGNHDVTDE
                     LILKSVQYLTSKEKKEPFMIQYKRSYTEGENIEFDATLYNAAGELINEPEVRLNIYND
                     EKKEFRYTFNRSNNAYQLNAGMLPPGRYTFSADTKAGDKVYKETGEFSVVPLLLENFE
                     STANHQLLNTISSNTGGIMVTPDKINLLIDAVQKNETVKPVSYYHKKLIELISLKSLL
                     AICLLLIAMEWFLRKRFGSY"
BASE COUNT         4210 a         2410 c         2433 g         3695 t
ORIGIN      
        1 atttatggca agacatcgga tcttgcaacc aatggtgatg gcagaatgta tgcagcaact
       61 caactaggat acatttatat aattaacccc ggaggtgtga cagataccat tcctttttta
      121 gacatcaaca gtaaagtaac cccaacatca ttaagtaata caggtgaaca gggattatta
      181 ggtcttgcct ttagcccaaa ttacaccaca gacagtagtt tttacgttta ctataccaac
      241 aaaactggta atggcaacag tgtgctagcc cgatacaaaa gaagtgctga tccaaatgtt
      301 gccgatactg taagtgcaga aattttattt gcattggcgc agccttatac gaatcataat
      361 ggtggctgcc tgaaatttgg taatgacgga tatttgtatc ttgctcttgg tgatggtggt
      421 gatgttggcg atccgggtaa cagagcacag aatatgaata tgtattttgg taaaatttta
      481 aggatagatg ttttacagcc tggagcatat accattccat caaccaatcc tttttttgga
      541 gttatgaatg ttaagaatga aatatgggca agtggattcc gtaatccatg gaaattcagt
      601 tttgacagac ttacacatga catgtggatt ggcgatgtag gtcaggatat atgggaggaa
      661 atagattttc aacaggctaa ttcaacaggt ggcgaaaatt acggatggcg ctgctacgaa
      721 ggtttagtta cttacaatac aagtgtaggg tgcataggaa attatatttc acctgtccac
      781 gtatatagcc atgcatctac cggtggatgt tctgttacag gcggatttgt atatcgtggc
      841 actgcctacc ctgctcttta tggatattat ttctttgccg atttctgtaa tggaacaata
      901 tatgctttag accctaacaa cggcaatgcc gttaccgttg caggaacatt tcccggacgt
      961 gcattcagca cctttggtga agatgataat ggtgaattgt atattgcaga cttagtgaat
     1021 cagaaggtgt atcaggttac agacacaact acttctattt ctgaatatgg taatgatgta
     1081 aataatgttg acatgtcttt caatcaaaat gagcaatcac ttcaaatgaa tttaacttca
     1141 aaaagcaaca atcaggcaac cctttatctt tatgatttaa aaggcacttt aattacagct
     1201 tggaagttga gtttaaaatc aggtaacaac acattcaaac tacctattaa tcttacctca
     1261 ggaatttatt taagcaacat taatagtgtc aacgcttcgc tcaacattaa gataccggta
     1321 ataaaataag cttactactt tttgattact tcaggtagag gtgttcctgc aggtatgaat
     1381 tttaatgagc tggtgtttac acagtgtctg gtgtttttag ctgtaaatcc ttcgcctaaa
     1441 aaaacatgac ctaaatgccc gtcacaatta ctgcaaacaa tttctgtgcg cataccatct
     1501 gcatcaggaa ttcgttttac agagcctttt atttcatcat caaaactggg ccaaccacaa
     1561 tgcgcctcaa acttatcagc gcttctataa agtggcgcat tacacctgcg acaaatataa
     1621 gtgcctacat cttttttaag ataataatca ccggtaaaag gcctgtccgt tactttaaac
     1681 aacaacactt tctcctcctg aggtgtcaat ttattgtatt ccatttttct gctgttttgc
     1741 gcaatgctta gctgccacat tcccattaag gtaactaagg caatattaaa tattctgatt
     1801 ttcattgaca aaatttattt gatacctcca cgctatcaat atgtacaaac acatttacag
     1861 aaacaaagtt aatacctgct atcagattca ttaaaaagct atattttagc agcaaattaa
     1921 agcatcaatt atgaattttg atttaatcat tataggaagt ggccccggag ggtatgtagc
     1981 agctatcaga gcatcgcagt taggacttaa aacagcaatt attgaaaaag aaaacctagg
     2041 tggtatttgc ctgaactggg gttgtattcc tactaaggca ttgctcaaaa gtgcaagtgt
     2101 ttttgaatac ctgaaccatg cttctgacta tggtattact gttgaaggtg gcaaagccga
     2161 ctttaatgct gttatcaaaa gaagtcgtgg cgttgcagat ggcatgagca agggcgttca
     2221 gtttctgatg aaaaaaaata aaatagaagt catcaatggt tttggaaaag taaagcccgg
     2281 aaaaagtgta gaagtaacag caaccgatgg cagcaaaaaa acatatacag cagcgcatat
     2341 tattcttgca acaggagcac gttcgcgcga gttgcctaac ctgaagcaag atggcaaaaa
     2401 cattattggc tatcgcgaag caatgagcct gcccgaacaa ccaaagtcaa tggtggtagt
     2461 gggttcaggt gctattggag tagagtttgc ctacttctat aatgctatgg gcacaaaagt
     2521 tactgtggta gaatttatgc ctaacattgt tcctgttgag gacgaagaag tgagcaagca
     2581 attagaacgc tctttgaaaa aagttggtat tgaaatcatg acaaacagtt ctgtagaatc
     2641 ggttacagta agtggcggaa cgtgcagtgt aaaaattaaa tctgcaaaag gtgagtcagt
     2701 tattacctgc gaaaaagtac tttctgcagt aggcataaca acaaatattg aaaacatagg
     2761 tcttgaagag tgtggcatca ttaccgataa aggtaaggtt gttgtaaacg atttttatca
     2821 gaccaacata cctggcattt acgcaatagg tgatattgta aaaggtcagg cattggcaca
     2881 tgtggcttct gcagaaggaa ttatttgtgt tgaaaaaatt gcaggccatc atccacaacc
     2941 actcgattac aacaatattc cgggatgtac ttattgcact cctgaaattg cttctgtagg
     3001 tatgacagaa aaagctgcta aagatgcagg cataggaata aaagttggta agttcccatt
     3061 cagtgcttca ggaaaagcta aagccggtgg acaaagtgat ggtttcgtta agcttatttt
     3121 tgacgaaaaa tatggtgagt tgcttggtgc acatttaata ggtgcaaacg ttactgaaat
     3181 gatagcagag ttggttctgg cacgaaaatt agaaacaaca ggtcatgaaa ttatcaaggc
     3241 tgtacatccg catcctacaa tgagcgaggc tattatggaa gctgctgctg ccgcctataa
     3301 tgaagtgata catttatagg attgcccttt gcttgttgta accctacaac aatttcgcaa
     3361 tttttaatta gctataggtg tgtttttcat tcttataaga taactacacc tcccctttcc
     3421 tgcttacgct gtaaatgtta aatttgcagc ataaaggtta ttctgatatg caggagtcaa
     3481 aaatttatac cccgaaacat aaaatccgta ttgtaactgc cgcttcgctg tttgatgggc
     3541 acgatgctgc catcaacatc atgcgcagaa tcattcagtc aagtggtgct gaggttattc
     3601 accttggcca cgacagaagt gcacaagagg tagtggaatg tgccattcag gaagatgcca
     3661 atgctattgc catgacaagt tatcagggcg gacacatgga atattttaag tacatgtatg
     3721 acttgctgaa agaaagaggc tgtgggcata taaaaatatt tggtggcggt ggtggcacaa
     3781 tacttccaga agaaataagc gaacttcagg actatggtat tgcccgcatc tatcatcctg
     3841 atgacggacg tgctatgggc ttgcaaggca tgattaacga tatgctccaa aaatgcgact
     3901 ttgccacagg agtaaattta aatggcgaaa taaaagagct gacaaagaaa aattcaaaag
     3961 ccattgcaag gctcatttct tgtgcagaaa ataatcaatc ggaatttgaa aaactatatc
     4021 ctgaaattac ttcttccctc tctaataaaa ccaaaaacag tcctgtatta ggaattacag
     4081 gaacaggagg cgcaggcaaa tcatctattg ttgatgaatt ggtaagaaga tttttgttgg
     4141 agtttaaaga taaatatatt gccatcattt ccattgaccc cagtaaacgc aaaacaggtg
     4201 gtgcattact tggcgaccgt atcagaatga acagtattcg caatccacgt gtgtatatgc
     4261 gctcgttggc aactcgtcaa agtaatcttg cattgagtcg ttatgttcag gaagcattag
     4321 atattgtaaa agccgcacag tttgatttaa ttatccttga aacatcaggt attggacaaa
     4381 gcgatactga gattaccgaa catagcaatg tgagtttata tgtaatgact ccggaatatg
     4441 gtgcagcaac acagttggaa aaaattgaca tgcttgattt tgccgacctc attgcactaa
     4501 acaaatttga taaacgcgga gcattagatg cattgcgcga tgtaaaaaaa caatacaagc
     4561 gcaatcataa gttgtttgac attggtgatg aaaaaattcc tgtcttcgga acaatagcct
     4621 ctcagtttaa cgatccgggt atgaatcaac tttataagtc tgtgatgaat accattgcag
     4681 caaaaaccgg agcagactta aaaagcgact atcacaccac agaagagatg agtgaaaaag
     4741 tgtttatcat tcctccgcat cgtacacgct atctttctga aatctcagaa aacaacagac
     4801 attatgacca gtgggttgaa gaacaatgta cacttgcaca gaagctttac tcgctctatg
     4861 aaagcatgca aataataaaa ggagcagctg ccgaagagct gaaaaaacaa tatgatatac
     4921 ttgaaagaag tttcgatggc aacaacaaaa aactattgga agattttcct gctaaaacag
     4981 aagagtacag taaagattat tatgaattta aagtacgtga taaagtttta aaaataaaaa
     5041 catttgatga atcactttcg cacaacaaaa tacctaaggt agcttttccg aaatacaaag
     5101 cttggggcga tttgctgaaa tggattttgc aggaaaatgt tcccggtgaa tttccgttta
     5161 ctgcaggtgt attccgtttt aaacgcgaag gcgaagaccc aacaagaatg tttgccggtg
     5221 aaggatgtcc tgagcgtacc aacaaacgtt tccattatgt ttcattaggc atgcctgcta
     5281 aacggcttag cactgccttc gatagtgtga cactctatgg caacgaccct gaccatcgtc
     5341 ctgatattta tgggaaaata ggaaacagtg gtgtaagtgt atgttgtctt gatgatgcaa
     5401 aaaaattgta tagcggattt aatctttgcg accctgcaac ttctgtatca atgaccatta
     5461 atggtccggc ttcaattatt gcagcctatt ttatgaatgc agctattgat cagcaatgcg
     5521 aaatttatat cagacaaaac ggattagaaa aagaagttga gaaaaaaatt gctgacgaat
     5581 tttcaaaaaa taaatcaagc caaccaaaat acaactacga aattccagaa ggaaacaatt
     5641 cgcttggcct tatgcttctg ggaataaccg gtgacaaagt tcttccaaaa gatgtttacg
     5701 ataaaataaa aaccgacaca ttaaagcagg ttcgcggaac tgtacaagca gatattctaa
     5761 aagaagatca ggcacaaaac acttgcatct tctctacaga gttttctctt agattgatgg
     5821 gcgacatgca acaatatttt attgacaaag ccattcgtaa tttttattct gtttccataa
     5881 gcggctacca cattgccgaa gccggtgcca atccaatttc gcaacttgct tttacattga
     5941 gtaatggttt cacttatgtg gaatattatt taagtcgcgg catggacatc aattcttttg
     6001 cgcctaacct ttcattcttt ttctccaatg gcatagaccc tgaatattct gttatcggac
     6061 gtgtagcaag gcgtatttgg gctaaggcta tgaaactaaa atacaaggcc aatgaacgtt
     6121 cgcagatgct taaatatcat attcaaacat cgggcagaag tttgcatgct caggaaattg
     6181 acttcaacga tatcagaaca acactgcagg ctctgtatgc tatttatgac aactgcaact
     6241 cacttcacac caatgcttat gatgaagcca tcaccactcc tacagaagaa agtgtgcgca
     6301 gggcaatggc aattcaactt atcatcaacc gcgaattggg tcttgccaaa aacgaaaatc
     6361 ctttgcaagg ttcatttata attgaagagt taactgattt ggtagaggaa gcagtactgt
     6421 cggagtttga ccgcattaca gagcgtggtg gtgtgctggg tgcaatggaa acaatgtatc
     6481 agcgtggaaa aattcaggaa gaaagtcttt actacgaatc gttaaaacac accggtgctt
     6541 atccgattat tggtgtcaat acatttttat cgagcaaagg ctcaccaact attttaccta
     6601 aggaagttat tcgcgccaca gaggaagaaa agcaacagca aattgctact ttaaaaaatc
     6661 tgcatgcacg ttatgcatct actgcacctg aaatgctgca cgacctgcaa ctcactgcaa
     6721 tccataatca aaatatgttt gaaaagctga tggaagtaag taagtattgt tccttaggac
     6781 aaattaccaa tgcattgttt gaagttggcg gacagtatag gaggaatatg taaattacaa
     6841 tttagtaatt aatagtaaaa tcattattcg tgtcaaccaa aacagtaact taaattaatc
     6901 taaaacctaa ttaatataag tgaatcaaga ccccaatgat gaacctgctt tagccttgtt
     6961 ggagcggata aagaaaatga agaatggaaa agggaagtat ggaaagaatg aaggttaaat
     7021 aagaaagtct ttatttaacc ttaatgacta caaacttaaa tagcaaaact tttatttaac
     7081 tttggctaaa gatgccaaag gcatccaacc tttatagaaa aacaaaacca acaaacaaca
     7141 cgactccgaa ggggtcgaac ataaaccata aataatgtca acctactcgc aaatttatat
     7201 tcaaattgtt tttgccgtaa aaggaagaga aagccttatt ctgccacaat gggaagagcg
     7261 tttacataaa tacattactg gcattgttca aaacaaagaa caaaaaatgc ttgccataaa
     7321 cggcatgtcc gaccatttgc attttcttat cggcatgaaa ccatcttgtt gtttatccga
     7381 tttggtaaga gaaataaaaa aatcatcaaa cgattttatc aaagttgaaa agctatcaaa
     7441 attcaagttt agttggcaag aaggctacgg tgctttttcg tatagccatt cccagttgaa
     7501 cgatgttatt gcatacatca ccaatcaaaa ggaacatcac cgaaagaaat cttttaaaga
     7561 agaatatgtt gaatttttaa agaagtttaa tgttgattac aatgaaaaat atttgtttga
     7621 atggatggaa tagttcgatg cctacggcat cgcgttttac aaaccgaatt aatgctataa
     7681 agctataatc cctttggggt tattgatgtg aaaacaacga aatatatgag caacgaaaat
     7741 acatcagcaa tagtaagtaa ggtatggagt ttctgtaatc cgctaagaga tattggcgtt
     7801 ggttatggcg actacttaga acaacttacc tatttgctgt ttttgaaaat gacttatgaa
     7861 ttcagcatct cccccctaca acagaaaact gccaatccca actgaaaaca attgggaaag
     7921 tttgacttca aggcgtggtg atgaactgga ataatatcat accaaaaccc taagtgtttt
     7981 gtcaaggcaa aaaggagtgt tggggcaaat atttgttcaa agtcagaata agattaaaga
     8041 acctgccatg ctttacaaaa tcattgacat gattgataaa gagcagtggg caacattggg
     8101 agcagacata aaaggaacca tttatgaagg tctattagag aataacccat aagacacaaa
     8161 aagggtgcag gacaatattt tacgctgaaa aagaatccgc tgaaattgga agaccttcgg
     8221 gacttcatta cttgctacaa tcctgacaac cgacacaaaa gaaaggaaac ttacaactca
     8281 gacacaaatc ctgaaggtcg ttggagaaag ttcacttatg atgaaattat caaccgtgac
     8341 aagacatcgc ttgacatcac ttggataaaa gataagtcac ttgccgacct tgacaactta
     8401 cccgacccgg atgttttggc aggagaaata attgaaaacc ttgaagctgg actggaaagt
     8461 ttcagagaaa taatgattac aataaatgga aaggactaaa aatatcctct tgctgatagt
     8521 tcctaaataa aaacagcatt cttctttttc aatctcctac atcttcttca atgcaagtat
     8581 tgaaaaatca gaatctattt ttctgatggt atttgtcagc acaccaagac cggcaatttc
     8641 catttcaact ttatctcctt ctttaagcca ctgtggttta tagtcaggat tattgaataa
     8701 ccctgtgccg ttcaactcta agaaacatcc tgtgccaaca gtaccgctgc caattacatc
     8761 accgggaaga atgtttacac cgtaggcaca acgctcaata atttctgcaa atgtccaatc
     8821 catacttcct acattacctt ccgacacctg aatgtcattg accttacaag tcatttttaa
     8881 attgtaatta tttcctgtgt gtccgctttt tgcaatggtc ttatatgatg caagttcatc
     8941 aggcgttact aaatacggac caataacagt agagaaatct tttccttttg cagggcctaa
     9001 gttgagtttc atctcttcca tctgcaattt gcgcgcactc atatcgttca taatcatata
     9061 gccggcaatg tagctatcgg catcttttgc ttttacatta atgcctttgc gaccaatgac
     9121 aattgctgcc tcaagttcga agtcaagttg ttcaaaatga tcgggcatgc aaaaaatatc
     9181 tccagggcct tgtatagcat tgtgattggt aaaataaaaa atcggatact gatcaaattc
     9241 cggaatcatc tctacacgtc tgttgcgcct tgctgcttca acatgttgcc tgaacgcata
     9301 accatcacgg caagaagttg gattaggcac cggtgccaat aaatttacat tagctactga
     9361 gagtccgtca gcatctgcat tgccatctga aagccattga ttcaactcca tagcagcatc
     9421 catcattagt tgttcgcctg ttaaaaaatc acgcatattg tcgggcaaca cagtttttcc
     9481 tctgcgttgt gacaattcgc taagattata aactttatca ctaagcaaga ttcccaatgc
     9541 cgttttattc tgattctgat aggttactaa tttcatattt tactacttta tggtgccaaa
     9601 attatcattc tgtatcgaag atgcctaata tagcttctat tgttgaaaac tgttttcata
     9661 tttcaattca aattcattct tagaacagct ataaaaattt atttttgaaa aaagtttaaa
     9721 tcatgatcag aaaaattctt gcaacacagg cgcttttatt atgcagtgct agtttcttat
     9781 ttgcacaaca tacccttgtt ttgaaaagtg gcgaaaaaat gacaggcaca gtgcagcaga
     9841 ttaaagatgg caatatcagc tttttattta aaggcaatcc catgaatttt aaagtgagtg
     9901 aagcctctgc catatttttt gatgcagcat cagaaacaaa agcagacaac tcagagatga
     9961 gtgccgatat gcgtggtgta aaatatgaaa tgcccggacg taccatcacc aagccgccaa
    10021 agattgaaaa tcttactatg gaaaaaggtg ttgttgtagt tgagatttct attgacaaat
    10081 atggccatgt taccaaggct aatccaggtg cagaaggcac aaccactaca agcagctatt
    10141 tgttaactaa agcaaaacag gcagcagaaa gtgtaatgtt taataactgc cctaaatgtc
    10201 cgttagatac caaaggcact attacactta ccttttaact ttgccataag caaagaataa
    10261 gaaattgcct aaggataaaa agttaattat tattgcaggg cctacagctt cgggcaaaac
    10321 acaaattgcc attgaagttg ctaaactgca tagcacagaa attgtttctt tcgattcgcg
    10381 acagattttt cgtgaaatga ctatcggcac tgccgtgcca gatgcccaac aaatgaatac
    10441 agtgaaacat cacttcattc attcgcattc tattgcagaa gatatgaatg caagtcgcta
    10501 tgccgatgaa gcaactaaca ccatcaatca gttgttagaa aaaaatgaaa cagttgttct
    10561 tacaggcggc acaggtatgt atatgcatgc tgctctcttc ggcctcgacc ctactccaaa
    10621 taccgattat caacttagag caagtcttga aaaagagtgt gctgaaaaag gattggagta
    10681 tctggctcat caattaaaaa ctcttgacga aaaaagctat catcaaatag acattcacaa
    10741 tccacgcagg gtcattcgtg cattagaaat tatttacagc tcaggcaaaa cacgttcatc
    10801 ttttattgaa gataaaaaca aaatatctac ttacaacttc acactttatg ttttaaatcc
    10861 cgacaggcaa ttactttatg ataacataaa tcaacgtgtg gatatgatga ttgaaaaagg
    10921 attggtggat gaagcgaaac tacttctacc ctaccggcat cttacggcat tgcacactgt
    10981 tggctacaaa gaattatttg agtatttcga tagaaacatt actcttgacc aagccatcga
    11041 aaaaataaaa caacatacac gcaactatgc caaaagacaa ttgacatggt tcaggcagta
    11101 tgatgatgcc gtttggatta caccggaaga aatgaaaaag aaaatcgaga aaaaaaatct
    11161 ttaataagaa ccgaaacgtt ttcttaagaa ccactccatt gcaattaaca acaagcagat
    11221 cgcaagtaaa cttttcaggc ttatcaattc aataagtttc ttatgataat acgaaacagg
    11281 tttcaccgtt tcgtttttct gaacagcatc aatcaacaaa ttgattttat cgggagtcac
    11341 cataatgcct cctgtgtttg atgaaatggt attgagcagt tgatgattgg cagttgattc
    11401 aaaattctcc agcaacaatg gcacaacaga aaactctcct gtttccttat aaactttatc
    11461 gcccgctttt gtatccgcac tgaaagtata gcgtcccggt ggcaacattc ccgcattcaa
    11521 ctgataggca ttgttgcttc tgttaaaagt gtatcggaac tcttttttct catcgttata
    11581 aatattcagg cgcacctccg gttcattaat taattctccg gctgcattat aaagcgtggc
    11641 atcaaactca atattctcac cttctgtata ggagcgcttg tactgtatca taaaaggctc
    11701 ttttttctct tttgaagtca ggtactgaac agattttaaa attaactcat cagtaacatc
    11761 atgatttcct gaaagtgcat attcattcaa ctgccagcgc cacaaacctt ccccaagtaa
    11821 aacacctgtt cgcattgttc ctgaagtaga aaaataccac agtggcattg acgattgtat
    11881 gcttccgata cgctgcgcta ataatgtatt ggtttctttc tctactttat aatttccaaa
    11941 aggcacaaat aatggaggta gttgcgacaa tactttttgc atatcgtctg acaaactaaa
    12001 caatccaaac tgatcgctta ccttagcata tacttcattc atctttgcaa caggctgatt
    12061 gattgttaca cccgtaccgg aattgttgaa tgcagcgaca ttcatttgcg cacctgcaat
    12121 gataaagtaa ggcacctctg ccgttttaat cttttgcaaa agtgttgttg actttgccga
    12181 tggaagctga tgcagaatta caagattgta gttgctgaaa ttttcatcgg cttgctcctg
    12241 aaaaataact ttggtttcat acagattact tttatcaagc acagatttta ttgcagcaat
    12301 atcaggatgt ggcgattgcg ccagcaacaa tattttcatt ttgcgttctg aaacgtcaac
    12361 ataaatatct gcttcattgt tgagcaaatt cttttcacca tccacttcag aaatgcgaat
    12421 tttatattta tgaatacctt ttctattggc agaaagtatg acaggtattt ttgcgaaata
    12481 attatttcct gatgcagaaa tcattctgct gaaaagtgtc agtgaatctt ctttaatggt
    12541 gagcgtaact gctgttcctg atgcctgcct catggtaaca cctacttcaa caggaaaatt
    12601 atttccgaga aaagccactt tattgtatgc aacatctgat attaacacat cacggttgat
    12661 ggttgtatcg ccaacagcaa tagtataaac aggaactttt aatttttctg ctgtataaac
    12721 aggattactt cccttattat aaattcca
//
LOCUS       DAOOOL010000075        12725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_455_length_12725_cov_170.971113, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000075 DAOOOL010000000
VERSION     DAOOOL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12725
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_455_length_12725_cov_170.971113"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKU82_07415"
     CDS             <1..662
                     /locus_tag="PKU82_07415"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOZ82736.1"
                     /translation="AGNPATWTTHLKQAGITVVHVVSNVKFALKSQAAGVDAVVAEGF
                     EAGGHNGREETTTLCLIPEVKDALQIPVIAAGGIGCGRAMMAAFALGAEGVQVGSRFV
                     ASIESSAHTNFKEKVINTSEGQTVLTLKQLTPVRLIKNKFSEQVDLAQQRCATREELT
                     QLLGRARAKKGMFEGDLDEGELEIGQVSSQINNIIPAGDIVKNIWNEFLIEKNRIANL
                     F"
     gene            666..1913
                     /locus_tag="PKU82_07420"
     CDS             666..1913
                     /locus_tag="PKU82_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ82737.1"
                     /translation="MVKFEKIILPNGLPVIVNYDPSTPLVAMNILYKVGARNEDAGRT
                     GFAHLFEHLMFGGSVNIPSYDEPLQRAGGENNAFTNNDFTNYYLTIPSQNVETAFWLE
                     SDRMLSLAFSEKSLDVQRNVVIEEFKQRYLNQPYGDVWLNLRPLAYKVHPYQWATIGK
                     DIEHIEDATLDDVKAFFHKWYCPNNAILTLSGNIKPELAFELAEKWFGEINAHELSKE
                     SLPKEPIQREYRNIMLERDIPANAVYKAFHTCSRFDNEFYATELLTDILCGSKSAVLY
                     HSLVKEQQLFNSINAYFTGDLDNGLMIVEGKVNDGVDVKHADHAIDTLLQLVVNDGVQ
                     EKEMKIVKQKTESTLVFSEVNIGNRALNLAYFEMIGSAEKINNQLQQYEVVTANNVQQ
                     VAKTIFKKENESSIYYCKKGVAV"
     gene            1910..3178
                     /locus_tag="PKU82_07425"
     CDS             1910..3178
                     /locus_tag="PKU82_07425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ82738.1"
                     /translation="MNRVIEPELKLTNKVDFTLPEKIILDNGIQAYVLNAGEQEVAKL
                     ELVFMAGEWYGEFSMLASAVNELLESGTAKRTAYEISQSFDQYGAYLNTSCSFDTASI
                     KLFTLSRFIPETFSLLLEIMSEAVFPDEEVKIFTDQSIQQLKINSQKVDYISRKTFNQ
                     MLFPDNHPYGRPASEADYKKLNSADLVLWHKQYYQPQNCVIFLSGRFNEDAVKSLNQL
                     FGPWKDSTLSKIVEKNFSIEKYKPERKYLEKKDALQTAIRIGCHTPNKLHPDYIPLTV
                     LSTVLGGYFGSRLMSNLREERGYTYGVGCAMVSWLHGGYFFISTQVGKQHRENALEQI
                     YLEIKRLREEKIPDNELNRVKSYMTGVYQRSLDGPMLVSDRIKSMVLYNQDFSLLNSY
                     LQIMNSTDSNQLLDLANRYFSNKMTEVSAG"
     gene            3295..10239
                     /locus_tag="PKU82_07430"
     CDS             3295..10239
                     /locus_tag="PKU82_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ82739.1"
                     /translation="MIRKHIIALLMFCTVFTVDVKASHLFGGEITWECIKSGPNIGKF
                     QFTLKVYRDCNGIPFTPPSAINVYGNPLVTSIPINAALTVITDLSPTGCGFTCANPAA
                     GATQEFVMKTNPVFLNGTPPASGWVFAIDDCCRNTLVNVGGGGQGFTIRAVMYPFNGQ
                     NMNPCYDSSPFFKERPNTYICIGYQYTYNPTAVDPELDSLYSDWTNPLEQPTSSPPAA
                     FNAPIINFTAPYTVNSQLPGNVTMNHNSGEVTFFVTTASSVTGNFATCLKVTAYKCQQ
                     KVAEIFRDYGVVLTTNCFVQNPPAINLPPAINLPFAGGTMKDTTVHVGDTVKFRIDAT
                     DFQRNIGSGAAFQTIRIDAYGDEFGVPINTNSGCGYPPCAKLDKLTPVISPLAAFINF
                     EWVTTCDHIIKPSGCLRERNTYYFIIKAQDNYCPAPAISTITIAITVVQSKPLEPPHV
                     RGASVLNTTGDVGLYWETPGVVNQLDTHHVFNSYQIYASNSYGGPYTLVDSVAGNKDF
                     YKQKGDTITATQLNTLIGANANAAPVYFYVRTKSLCNGDSISVPSDTISTMFVSTSIV
                     NCETQVNWNSLFKHAYPGSAALVYKIYREHPIGSPPVQIATVPNTWPNATYTDPFSKT
                     VCRDSVKYYVTIDDTLIIKSTPTVTRCVSKSNVTKQYIDNSFTASISPIGPTNLCPPN
                     CVNLTVSANPSPACFTTSYLWSNGATTSGITACAAGNYSAVITQSPSGCTSTTPIHVV
                     TLTPGPTANIAISGVSSVCPGDSVNLQFTFAGGAPYTYSYNVPGPGCVGTTVVNAVAN
                     SSPVTVRVPVNCNFNYTLNSVMGACAGTVSGSANATIKALPTASVVNTANDTVCNGGG
                     STISLAFTGTGPWNYVIKSSTGPDITATTASNPVSINVTPASTTTYTLFSVKDACTGT
                     VSGTRKIVVLPIPTAAMSITGNDTICSGSSTTIRVDFTGTGPFVGNIKDNTTGVLTPV
                     NTAASFVTLNVTPNATTTYSFQNFVSSAKACPGTIANSVVVAVKAIPTANISSVTNAT
                     ICKNDSVRMQVSFTGNGTPYTFGLVTNAGAPANVNAATNPYYFYVHPTTSTNYIVTTV
                     TDAFCSKSNINDTVAVTVNQLPTAVLSGTTTICAGQPTTLTINFTGTGPFTGSYVAST
                     GGTTNFNTSANPYTFNVSPSATTTYSLSATINDSKCSNNTNPATAIVTVNALPQASIS
                     GNKTICTGANDTLLVNFTSGNAPWTFYYKDGLGNTYGPVTTSNNPYKVVVSPTATTTF
                     QLDSVFSGVCKGSVNGIGSVTVRPLPTATFTTNNDTICNGNATNLTIQFTGTAPFSYQ
                     LQGQAVQNAATNPVTINVNPTTTTNYTLVQVNDQLCTSATSQQVKITVLNLPTAVLTT
                     TTPNLCTGVSGSITINFTGNGPFTTAYSNGTVNTPVVSNTNSIVIPVSPTVNTTYSLV
                     GNVNSMYGCNAPVNPATALITVRPLPTMTISGNPIICESTSANFNITFTGTAPFNYSY
                     FNGTSNVNGTTSTNPLTITVSPPVGTTTYTPVSIADQYCTGTALNGSSVVKVNPKPTA
                     TLFGNDTICVGSSSFIAIKFVGTPPFDYSYTTNGVSSGSLSSPVDSVSIPVAPNVNTT
                     YALSTSISDANCSNSNINQSVYMKVTPIPTAAITGTNAICSGGSTNLTINFTGEAPYN
                     YSYLAGGTTMGPFTTNNTSVTIPVSPTANTNYTLPATVNGNGCNGPASGNAMITVNAI
                     PQAVISTAKDSICKGDSTQLSIQFTGAAPFTYQLQGQGVTNATSNPHTFYVSPQSNTS
                     YVLTNITDNNCPAVINQNIGVKVLDNPTVAISTATPSLCKGNPGSITLNFTANGPYSV
                     TYNDGTSNFPVTSTSNSMTIPVTPTATTTYTLSGVVTGLLGCKTSATGATTVTVHELP
                     QAVISGNPVICLNEIAPIQIQFTGTAPFNYSYQNNVTGQINNGTTSQNPLTINLSPTT
                     TTSYNLTAISDKNCPGTQISGLANITVNPLPQPVITGVNQVCEGTPSVLSTTLPYQSY
                     QWSNATTGSTTAVTTTGTYTVEVTDINGCKKLSPPFAFIAHPYPVVNFTNDTSKTCQV
                     PRVNYFNQSLYQPGATFQWSLGENAVSNEVNPSHVYSQPGTYNVQLIVTNPFGCKDSI
                     EKQVEVIFYPLPVAQFTANPMVTNIFNGPVSFTDQSAYAVSWKWVFGEGDTLYDQNVT
                     HYFPEIGEFPVKLMIENISGCKDETQQTIVINPFWLPNAFTPNGDGRNEVFFDPGFAM
                     DYSGFNLRIFNRWGQMLFQSDSPSKPWDGSNGKGDLAPQGTYVYTLSVTNKGGKRHDF
                     NGTITLLR"
     gene            10461..11255
                     /locus_tag="PKU82_07435"
     CDS             10461..11255
                     /locus_tag="PKU82_07435"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOZ82740.1"
                     /translation="MNQYHELLRHVMTNGVEKSDRTGTGTKSVFGYQMRFDLQKGFPL
                     ITTKKLHTKSIIHELLWFLKGETNIEYLKQNGVSIWDEWADKNGNLGPVYGHQWRSWS
                     TPDGNGIDQISSLIDTIKNNPDSRRMLVSAWNVADVSKMALPPCHILFQFYVANKKLS
                     CQLYQRSADIFLGVPFNIASYALLVHMIAQVCQLEVGEFIHTLGDAHIYSNHFEQVEL
                     QLSRTPYQLPTLQMNPLVKDVFKFKFEDFYFSDYQSHPAIKAQVAV"
     gene            11260..11763
                     /locus_tag="PKU82_07440"
     CDS             11260..11763
                     /locus_tag="PKU82_07440"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOZ82741.1"
                     /translation="MLVSIIAAMDQNRLIGAENDLPWHLPVDMKYFKETTLGHYVLTG
                     RKNYESIPEKFRPLKGRTNLIVSNSKIQFPGAILVSSIKEGIEIARKAGEKELFIIGG
                     GQVFKQSIHLANRLYVTLIHHQFNGDVFFPQINWEQWEIISRKDVVPDENNKYACTFI
                     VAERISV"
     gene            complement(11774..12652)
                     /locus_tag="PKU82_07445"
     CDS             complement(11774..12652)
                     /locus_tag="PKU82_07445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ82742.1"
                     /translation="MANATIAITGAGGFLGKALTALLKKNNFEVVSISRSSGVDITKA
                     ETLTTIPDFDCLIHLAAHTFVPDSYNNTAAFFSINLNGTLNALELCKKRNARFIFSGS
                     YVYGQPNYLPVDENHPINMWNPYASSKIICEQLCHVYNKEFQVPVDILRIFNIYGPGQ
                     NSSFLIPKIIEGVFKGQLNLETLTPRRDFIYVNDVCNAFLQCLLIHSEEQTFNVYNIG
                     SGSSYSVQQIVDKVISFTGLNPTISCNEKRRAVEVEDVKADNSKIKRDKKWHPSVSFD
                     QGLKDMIEIYRNENDL"
BASE COUNT         4141 a         2260 c         2389 g         3935 t
ORIGIN      
        1 ctgctggtaa tcctgcaaca tggactactc atttaaaaca agcagggatt actgttgtac
       61 atgttgtaag taatgtaaaa tttgcattaa agtcgcaagc tgccggtgtt gatgcagttg
      121 ttgccgaagg ttttgaagcc ggagggcata atggtcgtga agagacaaca acattgtgtt
      181 tgattcctga ggtaaaggat gctttacaaa taccggtgat tgctgcaggt ggaattggat
      241 gtggtcgtgc tatgatggca gcttttgcat taggtgcaga aggtgtgcag gttgggtcgc
      301 gttttgtggc aagtatagag tcatcagcac atactaactt taaagaaaaa gttattaata
      361 cttcagaggg tcaaacagtt ttaacattaa agcaactgac acctgtgcga ttgataaaga
      421 acaaattcag tgaacaagtt gatctggcac aacagcgctg tgcaacgcgt gaggaattga
      481 cgcagttact cggcagggca agagcaaaaa aaggaatgtt tgaaggagat ttggacgaag
      541 gcgaattaga aattggtcag gtatcgtcac aaataaataa tattattcct gccggtgaca
      601 tagtgaaaaa tatttggaat gaatttttaa tagaaaaaaa ccgtattgct aatttatttt
      661 aatttatggt gaaatttgaa aaaataattt tgcccaatgg tttacctgtg attgtaaatt
      721 acgatccttc aacaccattg gttgcaatga atattttgta caaagttggt gcacgcaatg
      781 aagatgcagg cagaaccggt tttgctcatt tgtttgaaca tttaatgttt ggaggttctg
      841 taaatattcc atcgtatgat gaaccattgc agcgtgccgg aggtgagaat aatgctttta
      901 caaataatga ttttactaac tactatctga ctattccttc acaaaatgtt gaaacagcat
      961 tctggttaga gagtgaccgt atgttaagtc ttgccttctc agaaaaaagt ttagatgtgc
     1021 aacgtaatgt ggtaattgaa gaatttaaac aacgctacct gaatcaacct tatggtgatg
     1081 tgtggctcaa tctcaggcca cttgcatata aggtgcatcc ttatcaatgg gcaacaattg
     1141 gtaaagacat cgaacatatt gaagatgcaa cattggatga tgtaaaagca ttttttcata
     1201 agtggtattg tcccaataac gctatactta cattatcggg aaacataaaa cctgaacttg
     1261 catttgaact agcagaaaaa tggtttggtg aaataaatgc acatgaatta tctaaggaga
     1321 gtttaccaaa agaaccaata cagagagaat acagaaatat tatgcttgaa agagacattc
     1381 ctgcaaatgc agtttataaa gctttccata cttgttcacg atttgataac gagttttatg
     1441 ctactgaatt acttacagac atactctgtg gaagcaaatc agccgtgttg tatcactctt
     1501 tagttaaaga gcaacaacta tttaattcaa tcaatgccta ttttacagga gacttggata
     1561 atggattaat gatagttgaa ggaaaagtta atgatggtgt tgatgtaaag catgctgatc
     1621 atgctattga tactttgttg caacttgtag tgaatgatgg agtgcaagag aaagaaatga
     1681 aaatagtaaa acaaaaaaca gaatctactt tagttttttc agaagtaaat attggtaaca
     1741 gggcattaaa tcttgcttat tttgagatga taggaagtgc agagaaaata aataatcagt
     1801 tacaacaata cgaagttgtt actgcaaata atgttcagca ggtagccaaa acaattttta
     1861 aaaaagaaaa tgagtcatca atttactatt gcaagaaagg agttgcagta tgaatagagt
     1921 aattgaacct gaattgaaat taacgaataa agttgatttt acattacctg aaaaaattat
     1981 cttggacaat ggcatacaag catacgttct taatgcaggt gagcaggaag ttgcaaaact
     2041 cgaattggtt tttatggcag gggagtggta tggtgagttt agtatgttgg cctctgcagt
     2101 aaatgagttg ttggaatccg gaacagcaaa gcgtacagcc tatgaaattt cacagtcatt
     2161 cgatcaatat ggagcctatc tcaacacctc ttgttcattt gatacagcat caattaaact
     2221 ttttacgtta tcacgattta ttccggagac tttttcattg ttactggaaa ttatgtcaga
     2281 ggctgtattt cctgatgaag aagtgaaaat attcacagat caaagtattc agcaattaaa
     2341 aatcaatagt caaaaggttg attatatttc aagaaagact ttcaatcaga tgctatttcc
     2401 ggataatcat ccttatggga ggccagcatc agaagcagat tataagaagt tgaattctgc
     2461 tgatttggtt ttatggcata aacaatatta tcaacctcaa aattgtgtga tttttctaag
     2521 cggacgtttt aatgaggatg ctgttaaatc cttaaatcaa ttatttggac catggaaaga
     2581 ctctacacta agtaaaatag ttgagaaaaa tttttctatt gaaaaatata agcctgaaag
     2641 aaaatatctt gagaaaaagg atgcattaca aactgctatt cggattggat gtcatacccc
     2701 gaataaactt catcccgact atattccatt gacagtttta tcaaccgttt tgggcggata
     2761 ttttggttca aggctgatga gtaatttaag ggaagaacga ggttatacct atggggttgg
     2821 ttgtgcaatg gtgtcctggc ttcatggcgg atacttcttc atttctacac aagtgggcaa
     2881 acaacatcgt gaaaacgcgc ttgaacagat atatcttgaa attaagcgac ttcgtgaaga
     2941 aaaaatcccg gataatgagc tcaatcgtgt aaaaagctat atgacaggcg tttatcaaag
     3001 aagtttagat gggccaatgc ttgtttctga tagaataaaa tctatggttt tatacaatca
     3061 ggatttcagt ttactaaatt catatttaca aatcatgaat tctactgatt caaaccaatt
     3121 acttgacttg gcaaatcgtt atttttctaa taaaatgact gaggtttctg caggttaagt
     3181 tcaatgaatt ttttcaaacg gcaactctgt taaaaaaatc tcgtttaatt gttaaattta
     3241 tgatgtagaa atgaagatta gttttatatt tgtacaaaca aatttaatga agctatgata
     3301 cgaaaacaca ttattgcact tttgatgttc tgtacagtgt ttacagtaga tgtcaaagca
     3361 agtcaccttt ttggtggtga gattacttgg gaatgtatta aatcagggcc caatattggt
     3421 aaatttcagt ttaccttaaa agtttacaga gattgtaatg gtattccttt tacgccccca
     3481 tcagcaatta atgtatatgg aaatccattg gttacttcca ttccaattaa tgctgcatta
     3541 actgtaataa ctgacttaag tcccacaggt tgtggtttta cttgtgcaaa tcctgcagct
     3601 ggtgcaactc aagaatttgt aatgaaaaca aatccggtgt ttttaaatgg aactcctcct
     3661 gcttcaggat gggtttttgc aattgacgat tgttgcagaa atactttggt taatgttggt
     3721 ggaggtggac aaggttttac gatcagagct gttatgtatc cgtttaatgg acaaaacatg
     3781 aatccttgtt atgattcttc tccatttttt aaagaaagac ctaatacata tatctgtatc
     3841 ggatatcaat atacttacaa tccaactgcc gtagatcccg aattagattc actttatagt
     3901 gattggacta atccattaga gcaaccaaca agttctcctc ctgccgcctt caatgctcca
     3961 ataattaatt ttactgcacc ctatactgtt aacagtcagt tgcctggtaa cgtaacaatg
     4021 aatcataatt caggtgaagt aacttttttt gtaacaacag catcaagtgt cactggtaat
     4081 tttgcaactt gcctgaaagt tactgcttat aaatgtcaac agaaagttgc agagattttc
     4141 agagattatg gtgtagtgct tactactaat tgttttgtac aaaatccacc ggcaattaat
     4201 ttgccaccgg caattaattt accttttgcc ggaggaacta tgaaagatac tactgttcat
     4261 gttggtgata cggtcaaatt caggattgat gctacagact ttcaacggaa cattggttca
     4321 ggagctgcat ttcagacaat tcgtatagat gcttatggcg atgagtttgg tgtgcctata
     4381 aatacaaaca gtggatgtgg ttacccacct tgtgccaagt tagataaatt gacccctgta
     4441 atttcacctt tagctgcctt tataaatttt gaatgggtca caacatgcga tcacatcatt
     4501 aaaccctcgg ggtgtttacg agaaagaaat acctactact tcatcattaa agcgcaggat
     4561 aattattgtc cggcaccggc aatcagtact ataacgattg caattactgt tgttcaatct
     4621 aaacccttgg agccacctca tgttcggggt gcatcggtgc ttaatacaac cggtgatgtt
     4681 ggattatatt gggaaacacc gggtgtcgtc aaccaactcg atacgcatca tgtattcaat
     4741 tcctatcaga tttatgcatc aaatagttat ggtggcccat acactttagt agattctgtt
     4801 gcaggaaata aggatttcta taaacagaaa ggtgatacta tcactgccac acaactaaat
     4861 acattgattg gtgccaacgc aaacgctgca cctgtttatt tctatgtaag aacaaaatca
     4921 ttgtgtaatg gggatagtat ttctgttcct tcagatacaa ttagtacaat gtttgtcagt
     4981 acaagtattg tgaattgtga aacacaagta aattggaatt cgctttttaa acatgcatat
     5041 ccgggttctg cagctttagt ctataaaatt tatcgagaac atcctatagg ttctccacca
     5101 gtccaaattg caacagtgcc gaatacctgg ccaaatgcaa catataccga tccattttca
     5161 aagacagtat gtcgtgacag cgtgaagtat tatgtcacta ttgatgatac tttaattata
     5221 aaatcaaccc caacagttac acggtgtgtt tcaaaatcta atgttaccaa gcaatatatt
     5281 gataatagtt tcactgctag tatttcacct ataggtccaa caaatctttg tccgccaaat
     5341 tgtgtaaatt taaccgtttc ggccaatcct agtcctgctt gttttaccac ttcatattta
     5401 tggagtaatg gagcaactac ttctggtatt acagcttgtg cagcaggaaa ctattcagca
     5461 gttataacgc aaagtccaag cggatgtaca agtacaacac caatacacgt tgtaacatta
     5521 acacctggtc caactgcaaa tattgcaatt tcaggtgtaa gtagtgtttg ccccggtgat
     5581 agtgtgaatc ttcagtttac atttgcaggt ggtgctcctt atacatatag ttataatgta
     5641 cccggcccgg gttgtgttgg aacaactgtt gttaatgcag tagccaattc atcaccggtt
     5701 acagtcagag tacctgttaa ttgtaatttt aactatacac tcaattctgt aatgggtgct
     5761 tgtgcaggaa cagtaagtgg aagtgcaaat gcaacaataa aagcattgcc aactgcaagt
     5821 gttgtaaata ctgcaaatga cactgtatgc aatggaggtg gttccactat ttcacttgca
     5881 tttacaggta ctggtccttg gaattatgtt attaaaagca gtacgggtcc agatattaca
     5941 gcaacaacag cttcaaaccc tgtttcaata aatgttactc ctgcttccac aacaacctat
     6001 acattgttta gtgttaaaga tgcatgtaca ggaaccgttt ccggaaccag aaaaattgtt
     6061 gttcttccaa taccaactgc tgctatgtct attaccggaa acgatactat ctgtagcggc
     6121 agctcaacta caattcgtgt tgactttaca ggcacaggcc cttttgttgg aaatattaaa
     6181 gataatacaa caggtgtttt aacaccggta aatacagcag catcttttgt gacactcaat
     6241 gtgactccga atgcaacaac tacttattca ttccagaatt ttgttagctc tgctaaggct
     6301 tgccccggta caattgctaa tagtgttgtg gttgctgtaa aagcaattcc tacagcaaac
     6361 atttcaagtg ttaccaatgc cacaatctgt aaaaatgaca gcgtgcgcat gcaggttagt
     6421 tttacaggca atggaacgcc atacacattt ggtttggtga ccaatgccgg tgcacctgca
     6481 aatgtaaatg cagctacgaa tccctattat ttttatgtgc atcctacaac gagtactaat
     6541 tatatagtta caacagttac tgatgcattt tgcagtaagt caaatatcaa tgatacagta
     6601 gctgtaacag taaaccaact gcctactgcc gtactttccg gaactacaac aatttgtgcc
     6661 ggtcagccaa caactttgac aattaacttt accggaacag gtccatttac aggaagctat
     6721 gttgcaagta caggtggcac caccaatttt aatacttcag caaatcctta cacatttaat
     6781 gtttcgccaa gtgcaacaac aacgtattca ttaagtgcta ccattaatga ttcaaaatgt
     6841 tctaataaca ccaatcctgc aacagcaatt gtaacggtta acgcattgcc gcaggcatct
     6901 atcagcggca acaaaacaat ctgtaccgga gcgaatgata ctttattagt aaactttact
     6961 tcaggtaatg caccatggac attctattac aaagatggat taggaaatac ttatggtccg
     7021 gttacaacaa gtaataaccc atataaggta gttgttagtc caacagctac tactacattt
     7081 caacttgatt ctgtatttag cggagtttgt aaaggaagtg taaatggaat tggtagtgtt
     7141 actgtgcgac cgttgccaac agcaacattt acaactaaca acgatactat ttgtaatggt
     7201 aatgcaacta acttaacaat tcagtttacc ggtactgcac cattcagtta tcagttacaa
     7261 ggacaagcag tgcagaatgc agctaccaac cctgttacca tcaatgttaa tccaacaact
     7321 actacaaatt acacattggt tcaggtaaat gatcagcttt gtacaagtgc aacaagtcaa
     7381 caagtaaaaa ttacagttct taatttacca actgctgttc tgacaacaac aacgcctaat
     7441 ttatgtacag gagtgagtgg aagtattacc ataaacttta caggtaacgg gcctttcact
     7501 acagcatact caaatggaac tgtaaacaca cctgttgtaa gcaatacaaa ttctattgtt
     7561 attccggttt ctccaacagt taacacaaca tattcattag ttggaaatgt taacagcatg
     7621 tatggttgta atgcacctgt taatccggca actgctttaa ttactgtgag accattgcca
     7681 acaatgacta tcagtggtaa tccaataata tgtgagagta catcagcaaa tttcaacata
     7741 acatttaccg gtacagcacc attcaattat agttatttta atggaacatc taatgtaaat
     7801 ggaacaacaa gtacaaatcc attaacaata accgtaagtc cgcctgttgg aactacaacc
     7861 tatactcctg ttagtattgc cgatcaatat tgtacaggca cagcgttgaa tggttcatct
     7921 gttgtgaaag ttaatccaaa accaactgca acattatttg gaaatgatac catttgtgta
     7981 ggaagcagtt ctttcattgc aattaaattt gtgggtacac cgccattcga ctatagttat
     8041 acaacaaatg gcgtttcatc gggtagcctt tcttcgcctg ttgactctgt ttcaattcct
     8101 gttgcaccaa atgttaatac aacttatgca ttgtcaacaa gtatttctga tgctaattgc
     8161 tctaacagca atatcaatca gtcggtttac atgaaagtta ctccaatacc gacagcagca
     8221 ataacgggaa ccaatgcaat ttgtagtgga ggttcaacaa atctcaccat caattttacc
     8281 ggagaagcac cttataatta ttcttacttg gccggtggaa ctacaatggg accatttaca
     8341 accaataata cttctgtaac aattcctgtg agtccaactg ccaatacaaa ttatacactt
     8401 ccggcaacag ttaatggaaa tggatgtaac gggccggctt caggtaatgc aatgattaca
     8461 gtcaatgcaa taccacaggc ggtaatttct actgcaaaag attcaatctg taaaggtgat
     8521 agtacacaat tatcaattca atttacaggt gctgcaccgt ttacatatca gttacaggga
     8581 caaggtgtta ctaacgcaac atctaatccg catacattct atgtttcgcc acaaagcaat
     8641 acatcttatg tactgacaaa cattacagat aataattgcc ctgcagtaat taatcagaac
     8701 attggtgtta aagtgctgga taatccaact gtagctattt caacggcaac accttcattg
     8761 tgtaaaggaa atccgggaag tattactctt aactttactg caaatgggcc ttatagtgta
     8821 acttacaatg acggcacatc aaacttcccc gtgacgtcca caagtaattc aatgacaatt
     8881 cctgttacgc caacggcaac tactacatat acattgtccg gagtagttac aggtttatta
     8941 ggctgtaaga cttctgcaac aggagcaaca actgttacag tacatgaatt accacaagct
     9001 gttatttcag gcaaccctgt aatatgttta aatgaaatag caccaataca gattcagttt
     9061 acgggaacag ctccatttaa ttatagttat cagaataacg tgacaggaca gattaacaat
     9121 ggaacaacat cacaaaatcc attaacaata aatcttagtc caacaactac cacatcgtat
     9181 aatctgacag caattagtga taagaattgc cccggaacgc aaataagtgg tttagcaaat
     9241 attacagtta atccgttgcc gcagcctgtc attacaggtg taaatcaggt ttgtgaaggt
     9301 acaccttctg ttctatcaac aacacttcct taccaatctt atcaatggtc taatgcaact
     9361 acaggaagta caacggcagt tactactaca ggtacatata cggtagaagt tactgatata
     9421 aatggttgta aaaaattatc tccaccattt gcatttattg ctcatccata tcctgttgtt
     9481 aattttacaa acgatacaag caaaacatgt caggtgccaa gagttaacta cttcaatcaa
     9541 tcattgtatc agcctggagc aacattccaa tggagtcttg gtgaaaatgc tgtttccaat
     9601 gaagttaatc cttcacatgt ttacagtcag ccgggtactt ataatgtgca gttgattgtt
     9661 accaatccat ttggatgtaa agattcaata gagaaacaag tagaagtaat tttctatcca
     9721 ttgcctgttg cacaatttac agcaaatcca atggtgacaa atattttcaa cggaccagta
     9781 agtttcactg atcagtctgc ctatgcagtt agttggaaat gggtgtttgg tgaaggagat
     9841 acattatatg atcagaatgt tacccattat ttcccagaga ttggagagtt tcctgttaag
     9901 ttgatgattg aaaacatttc tggttgtaaa gatgaaacac aacagaccat tgtgattaat
     9961 ccattctggt taccaaatgc atttacacca aatggtgatg gacgaaatga agtgttcttt
    10021 gatcctggtt ttgcgatgga ttattcagga tttaatttac gtattttcaa ccgttgggga
    10081 caaatgctgt tccagtcaga ttctccatca aaaccatggg atggctcaaa tggcaaaggt
    10141 gatttagcac ctcagggaac ctatgtttat acacttagtg ttaccaataa aggtggaaag
    10201 cgacatgatt tcaatggaac aattactttg ttgagataag attatgtaaa aattagattg
    10261 tttgataaat aacagccgtt aaaatcaatt acagatttta gcggctgttt agttttacga
    10321 agattaaact ttattgctca caaattgagc aaatattaaa tgtaacattg tgaataaaaa
    10381 caacctctac ggatgcatag tcagacatga aatccatttg tttaatttaa ttttgaagcc
    10441 ttaaaattgt ttataataga atgaatcagt atcacgagtt actcaggcat gtaatgacca
    10501 atggcgtgga aaaatctgac cgcacaggta caggtactaa aagtgttttt gggtatcaga
    10561 tgcgatttga tttgcagaag ggttttccgc ttatcacaac aaaaaaactt cacaccaaat
    10621 caatcataca tgaattatta tggttcttga aaggcgaaac gaatattgaa tacttaaagc
    10681 aaaatggtgt ttcaatatgg gatgagtggg ctgataagaa tgggaatctc ggtcctgtat
    10741 atggacatca gtggcgttcg tggtcaactc ctgatggtaa tggaattgat cagataagtt
    10801 cgttgattga cacaattaaa aacaatcccg actcgcgtag gatgcttgtg agtgcatgga
    10861 atgttgctga tgtatctaaa atggcattgc caccatgtca catacttttt cagttttatg
    10921 tagctaataa aaaattgtca tgtcagcttt atcaacgtag cgctgatatt tttcttggtg
    10981 tgccctttaa catagcctct tatgctttgc ttgtacacat gattgcacag gtttgtcaat
    11041 tggaagttgg tgagtttatt catacccttg gcgatgcaca catttattcc aaccattttg
    11101 aacaggtaga actccaactt tcgcgaacac catatcaatt gccgacactg caaatgaatc
    11161 cattggtaaa agatgtcttt aagtttaagt ttgaagattt ttatttttcg gactatcaat
    11221 cgcatcctgc aattaaagca caggtagcag tttaatttta tgttagtttc aataattgct
    11281 gctatggatc agaatcgttt aattggtgct gaaaatgatt tgccatggca tttgcctgtg
    11341 gacatgaagt atttcaaaga aactacttta ggtcattatg ttcttacagg tagaaaaaat
    11401 tatgagagta ttcctgagaa attcagaccg ctgaaaggta gaacgaattt aattgtatcg
    11461 aattctaaaa ttcaatttcc gggagctatt ttggtttcaa gtattaaaga aggtattgag
    11521 atagcaagaa aggcagggga gaaggagctt tttattattg gtggcggaca agtgtttaaa
    11581 caatcaatac atcttgcaaa taggctttat gtcactttga tacatcatca atttaatgga
    11641 gatgtttttt ttcctcaaat taattgggag caatgggaaa ttatatcaag aaaagatgtt
    11701 gtgccagatg aaaacaataa atatgcttgt acttttattg ttgctgaaag aatttcagtt
    11761 taatgataat aagttataaa tcattttcat tccggtaaat ttcaatcata tctttaagtc
    11821 cctgatcaaa tgaaacggac ggatgccatt ttttatcacg cttaattttg gagttatctg
    11881 cttttacatc ttcaacttca acggcacgtc ttttttcatt acacgatatc gttggattta
    11941 aaccagtgaa agaaatcacc ttgtcaacta tttgttgtac actgtagcta ctgccactac
    12001 caatattata aacattaaat gtttgctctt cagaatgaat taacaaacat tgtaagaaag
    12061 cattacaaac atcatttacg taaataaaat ctcgtctagg tgtaagtgtt tcaagattta
    12121 actggccttt aaaaacacct tcaattattt taggaattaa aaaagaagaa ttctgacccg
    12181 gaccataaat attaaaaatt cgtaaaatgt caacaggaac ctgaaactcc ttattataaa
    12241 catgacacag ttgttcacaa ataattttac ttgatgcata aggattccac atgtttatag
    12301 ggtgattctc gtcaacagga aggtagtttg gttgaccata aacatacgag cctgaaaaaa
    12361 taaatcgtgc attgcgtttc ttacataact caagtgcatt aagtgttcca tttaaattta
    12421 tggagaaaaa tgctgctgta ttattatatg aatccggaac gaaagtatga gctgcaaggt
    12481 gtatgaggca atcgaaatcg ggaattgtag ttaatgtttc tgcttttgta atatcaacac
    12541 cggaggatct tgaaatacta actacttcaa aattattctt ttttaataaa gcggttaatg
    12601 ctttaccaag aaacccaccg gcaccggtta ttgcaattgt agcattagcc atttgatttt
    12661 ttctctattg cttccatctg ttggcaggca cgttggtaat cttccaagcg gccaatgtct
    12721 aacca
//
LOCUS       DAOOOL010000076        12571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_465_length_12571_cov_125.844679, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000076 DAOOOL010000000
VERSION     DAOOOL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_465_length_12571_cov_125.844679"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..2054)
                     /locus_tag="PKU82_07450"
     CDS             complement(243..2054)
                     /locus_tag="PKU82_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82743.1"
                     /translation="MSHSDLHRFHIPVMGLAFTVDTPVKVARYGISSVVSIIEHNMLE
                     VMREFYYKQNGKDFTAITKKDEDYRAKRITSYLNIINEIVLKQITDLRNESFETGKEI
                     NKYFEMLPDTSEAKLLYRKMQASTNQVEKEQLQQELRKFIKSGAIDVNIMTKVDRSNY
                     DANGQPLAQEFSDAQTALRGFANSNLSSSMVFSAGLNPRLYSYCEKFDDFFPDETGFV
                     KKKITLKVSDFRSAQVQGRFFAKKGIWISEFRVESGLNCGGHAFATDGLLLGPILEEF
                     KLNKNALYEELFNTCQTALAQKNRPQYKEKPKMKVTVQGGIGTSQENDFLYQHYEVDL
                     TGWGSPFLLVPEATNVDQETLGLLATASKDDYYLSDASPLGVPFNNFRKSSAERQRKE
                     RIAKNRPGSACYKKFLAFNTEYGGEPICTASRKYQHLKLQELKQNNVEPSQKQIDDLL
                     IKDCLCEGLASPVMLVNNVPVPHNLNAVTICPGPNLAYFSGTFTLQEMINHIYGRANV
                     LNSLYRSHMFVNELKLYIDYLKKDIQKNAATLNDKKEKQLQLFKENLSKGIEYYRHLA
                     VKLKQETKQSIENFKNDLMECELNLKMLQINSPVPAL"
     gene            complement(2248..2901)
                     /locus_tag="PKU82_07455"
     CDS             complement(2248..2901)
                     /locus_tag="PKU82_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82744.1"
                     /translation="MDESTNREKVLKKVRSALIAKTPNPYPNLDMDKVVYNSTSYSPE
                     IQFAENFTQQGGQFVLCNGRVELMETILGVCDANDFTEVVCVDRNLSAFFDEFEFPHQ
                     SVFSEEDDTDVQVVIIPCECLVVRNGLIVLSEDNIYQKALLRATQHLVIIAHTQQLIP
                     DITEALAYVRSQNDNNLPSELHFATPNFLNQSHTEVETNTLVKNITVILIQPYDSEL"
     gene            complement(2905..4920)
                     /gene="ftsH"
                     /locus_tag="PKU82_07460"
     CDS             complement(2905..4920)
                     /gene="ftsH"
                     /locus_tag="PKU82_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOZ82745.1"
                     /translation="MPKPPGKGFNFSWIYAIVFLLIFVIWVLGLGDKPKLTNMNEFEK
                     TMLATHDVEKIEIVNRERVEITIKKDKLNQEKYKAVSHKPFGNGYNPGPHYYMPIGDV
                     GNFESKLEAIQKDFPENERIQPEYNNNDRSILEVLFWNLLPFIIIAVIWIFIMRRMGG
                     GGAGSQIFNIGKSKAQLFDKDLNVKVTFNDVAGLEEAKVEVMEIVDFLKNPKKYTQLG
                     GKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDLFRQ
                     AKEKAPSIIFIDEIDAIGRARGKAPSFSANDERESTLNQLLTEMDGFGSNTGVIILAA
                     TNRADILDRALLRPGRFDRQIYVELPDLTGREEIFKVHTKTLKIDHTVDIKFLARQTP
                     GFSGADIANLCNEAALIAARKNKTAIGKDDFLSAVDRIIGGLEKKNKIITKHEKSVIA
                     YHESGHATVSWLLEHAHPLVKVTIVPRGNSLGAAWYLPEERQLTNKEQIFDEICAALG
                     GRAAEEVIFGTISTGALSDLEKITKQAYASVSIFGLNERIGNISYYDSSGQQDYSFSK
                     PYSEKTAEIIDEEVKKLIDLAYQRTKKLLVDNKEKLDKLAHRLLEKEVIFKEDLEEIF
                     GHRPWGESEKATFNSFITDGEPEPEKTVPPAVDSPPPSNIPEGSIQV"
     gene            complement(5043..5501)
                     /gene="rsfS"
                     /locus_tag="PKU82_07465"
     CDS             complement(5043..5501)
                     /gene="rsfS"
                     /locus_tag="PKU82_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOZ82746.1"
                     /translation="MAKRTNPAKDKDETTPVKAATLKKTKARQPKRKLKDNTEKLLTV
                     IVDSIEDKKGEHIVVLNLKELNNAMADYFIICQASNKTQVEAIVRNIEEKVEKKLKET
                     AAHIEGKQNAEWILLDYFNVIVHVFLADKRDFYSLEKLWADAPLVKFAAQ"
     gene            5573..6313
                     /locus_tag="PKU82_07470"
     CDS             5573..6313
                     /locus_tag="PKU82_07470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOZ82747.1"
                     /translation="MFKNSIWFNLHETESTNSFLHEKLMSEKLPEGSVVTADYQTKGR
                     GHRGSSWHSEAGKNLLMSIVVYPEFLKATEAFMLSKCMALATYDLLADYSNKVQIKWP
                     NDILIEGKKVAGILIENILRGSEIYASIIGVGINFNQQLFEKELTTATSVFMDSGVKA
                     DTQINAVKLHENIEVYYQKLQQKKFAEIDQLYLTNLYKLNESALFKDADGQFQGKIRG
                     LSSMGLLIVERENGEKHLYDVKEISLVR"
     gene            complement(6330..6746)
                     /locus_tag="PKU82_07475"
     CDS             complement(6330..6746)
                     /locus_tag="PKU82_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOZ82748.1"
                     /translation="MWWIYAILSALFASLTAIFAKLGVANINSNLATGIRTIVVLVMI
                     WIIILLKGETKGINTLSKQNIIFLILSGIATGLSWLFYFKALQTGNVSQVAPVDKLSI
                     ALTIILAMIFLGETLTIKTAIGASLIIAGTLVMVLN"
     gene            complement(6832..7800)
                     /locus_tag="PKU82_07480"
     CDS             complement(6832..7800)
                     /locus_tag="PKU82_07480"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOZ82749.1"
                     /translation="MIHKSHAVKLVLLLTLFLSCKKEKLDLVFEEISTPTNFNLNNLF
                     ILNDSIAFVCGGQTGNGIILRSSDGGLSWQILNNQFEYNLHSIFFIDNQHGFCGGDST
                     HVYQTNDGGINWQLHFDWEGIPFQYNSPLHSAYFVNKDTGFFAGGKNFDRGIIYSTTD
                     GGEHWNVAGFEHELRCITPTAAGILSGGYGTMLLSKQNFEFATLDCDRSFYTGFAFSN
                     SSEGVACSYNGALYHTSDGGNTFSLTDKKNQTFGKREHFLCIDARKNKIITSGLSGFT
                     AISIDGGENYDSGYSLNNSRINAIKFLNDTIAIATSDNGKIFRIHL"
     gene            complement(7800..8423)
                     /locus_tag="PKU82_07485"
     CDS             complement(7800..8423)
                     /locus_tag="PKU82_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82750.1"
                     /translation="MSTNKTDSKERSIRVSRIERIHPYRTILFFGILISSLLFLTLSF
                     LFLITQNENNNMPPALPKVFTLSTILLLFGSYTISKAVSAFRKDSFDKLLRATILTTI
                     ITSLFLVTQIIGLHAMQKTGYYTNMPFNSHYFFGIIALHMVHALATVAYLIFITYNAF
                     HQSHDVIDSLLFFSDQGQLLKLNSAMVFSHFVNFSWLAIFLMFLFSF"
     gene            complement(8487..9092)
                     /locus_tag="PKU82_07490"
     CDS             complement(8487..9092)
                     /locus_tag="PKU82_07490"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOZ82751.1"
                     /translation="MFSEAFMQTDLYSWVVLPVLIFLCRMCDVTLATLRNIFLSRGVR
                     KIIPVIGFVEVLIWLLAVSSIIKNLHNIMCYIAFAGGYSMGILVGISIEKRLALGTQI
                     IRVITSRQSQKLLEALRAQNLGVTEIDGMGSQGPVKILLIVAKRKNIATILEIVNEHH
                     GSSFFTVEDVRSVEQGIFPIKAGEGKIQYLRRIFPTFKPGR"
     gene            complement(9085..9774)
                     /locus_tag="PKU82_07495"
     CDS             complement(9085..9774)
                     /locus_tag="PKU82_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOZ82752.1"
                     /translation="MKRIVVLTGAGISAESGIKTFRDSNGLWEEHRIEDVATPQAWQR
                     NPKLVTEFYNQRRRQVMEAQPNKAHLALVDLEKHYQVVVITQNVDDLHERAGSKHIIH
                     LHGEILKMRSEEDPTMIYQSNGWEMNWDSTNEHGHRLRPHIVWFGEMVPKMDEAIFQT
                     MKADILLVIGTSLEVYPAAGLIDYTSQTCEKYIIDPKARELNHIHNLTVIQNTAGEGV
                     PALVNKLIKNV"
     gene            complement(9828..10784)
                     /gene="fmt"
                     /locus_tag="PKU82_07500"
     CDS             complement(9828..10784)
                     /gene="fmt"
                     /locus_tag="PKU82_07500"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOZ82753.1"
                     /translation="MNDLKILFSGTPDFAVESLKILFENGYNICGVITAPDKPAGRGM
                     QMQQSAVKKYAQSKGLLILQPEKLKDAAFLSQVASLKADLHIVVAFRMMPEQLWSMPR
                     LGTLNLHASLLPQYRGAAPINRAIMNGETTTGVTTFFLKHEIDTGDIILSDKVDIGTT
                     EIAGELHDRLMHIGAALVLKTVQQIEKGNLSTTPQNSIASTIKHAPKIFKNDCIINWS
                     DKTSTVFNQIRGLSPYPGAFTLLEDKNGKTLTLKIYIASVVENKELLEAGKIISDNKS
                     FLNLATSDGVISIKELQLEGKKRMNVEDFLRGFSINDYTVKK"
     gene            complement(10799..11104)
                     /locus_tag="PKU82_07505"
     CDS             complement(10799..11104)
                     /locus_tag="PKU82_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82754.1"
                     /translation="MEKIKHVLLLLLIYMLTSCKSAKQETVNQNLETVTVTDMRNLDG
                     CGFLLKTVDQKMLIPDNLDASFQKNNLKLEISYKKTQVMTTCMAGQTITLLYCRKAK"
     gene            complement(11092..12414)
                     /locus_tag="PKU82_07510"
     CDS             complement(11092..12414)
                     /locus_tag="PKU82_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOZ82755.1"
                     /translation="MQVKSTYLNIWQIAYPIMLASVAQMLVGLTDTAFLARVGEVELG
                     ASAIAGVFYFVLVMLGMALSIGSQILMSRKAGENDRLAIGNYFDHSVVILLVLAVLLF
                     FIMFFAAPAFFNLVLSSKDVADASITYIHNRSFGIFFILLVNCFRAYYTSISATRFIT
                     YSALVLTVSNFILDYVLIFGKLGFEAQGIAGAARASAIAEVLACIYLFFITFYHKENS
                     SFGLFRFDNINMTRINEIIFISIPVFFQNLLSMGAWFLFFVFIERLGQHELAVSNVVR
                     SAYMVLMTPIWGYASAANSMVSNLIGQNKQDEVIPLIKKIIVLSLASSTIILLINLFD
                     FKLLLRIISSDMMLLHDATPSYIVTWCAMFFFSVSMVLLSAISGTGKTNVAFIIEVIN
                     IAIYITYAFIAAVVLKAQLHVVWYVEILYWVIMGLCSWLYLRTNKWRK"
BASE COUNT         3797 a         2357 c         2284 g         4133 t
ORIGIN      
        1 ttatgaatga aatgcaaggt attcaaagca caggaagcca tcagaaaatg ataggtacac
       61 aattgcttgg tgcaggtgtt tattttctga ctattacaac agataatttt agccagacac
      121 aaaaggtgat tgtcaggaag aattaaattt tatttattca gataatgagt aaatcaaaaa
      181 agcagtttca catcttaata gtgaaactgc ttttttgatt taaatattat tattagttga
      241 acctacaaag caggtacagg agaatttatt tgaagcatct ttagatttag ctcacattcc
      301 attaaatcgt ttttaaagtt ttctatcgat tgttttgttt cttgtttgag tttaacagca
      361 agatgcctgt aatattcaat accttttgaa agattttctt tgaataactg caattgcttt
      421 tcttttttgt cgtttagtgt tgcagcattt ttttgaatgt cttttttaag ataatcaata
      481 tagagtttga gctcatttac aaacatgtgt gaacggtata atgagttcaa aacatttgct
      541 cgtccataga tatggttaat catttcttgc agggtaaagg ttcctgaaaa ataagccaga
      601 tttggtcccg gacaaatagt tactgcgttc agattatgtg gaacgggtac attatttacc
      661 agcattacag gtgaagccaa tccctcgcat aagcaatctt taataagcaa atcgtcaatt
      721 tgtttttgtg atggctctac attattctgt tttagttcct gtagcttaag atgttgatac
      781 tttctggatg cagtacatat tggttctcca ccatactctg tattgaatgc cagaaatttt
      841 ttataacatg cactaccggg tctgttttta gcaatgcgtt ctttgcgctg tctttcagca
      901 gatgattttc tgaaattgtt aaacggcaca ccaagaggtg atgcatcact cagataataa
      961 tcatctttac ttgcagttgc taataatcct aatgtttcct gatcaacatt ggtagcttcc
     1021 ggaactaata aaaacggact tccccatcct gtaaggtcaa cttcatagtg ttgataaaga
     1081 aagtcgtttt cctgagatgt accaattccc ccctgaacag taactttcat cttaggtttt
     1141 tccttatact gtggtctgtt tttctgtgcc aaagcagtct gacaagtgtt gaacaactct
     1201 tcatacaatg catttttgtt tagcttaaac tcttccagaa taggacctaa taataatcca
     1261 tcggtagcaa atgcatgacc accgcaatta agtcctgatt ctactctgaa ttcagaaatc
     1321 catattcctt ttttcgcaaa gaatcgtcct tgtacttgtg ccgatctgaa gtcgcttact
     1381 tttagtgtaa ttttcttttt aacaaatcct gtttcatcag gaaagaaatc atcaaacttt
     1441 tcgcaatagg aatataaacg tggattaagt cctgcagaaa aaaccataga agacgaaagg
     1501 ttgctgttgg caaaaccacg aagagctgtc tgcgcatcag aaaattcttg tgctaaaggt
     1561 tgtccgtttg catcgtagtt acttcggtca accttagtca tgatgtttac atcaatagca
     1621 ccactcttta taaacttgcg caactcctgt tgtaattgtt ctttctctac ctgatttgtg
     1681 ctagcctgca tcttcctgta aagaagcttt gcttcagagg tatcaggtaa catttcaaaa
     1741 tatttattga tttctttacc tgtttcaaac gactcattgc gtaaatcagt aatttgtttt
     1801 aaaacaattt cgtttattat gttgaggtaa gaggtgattc tttttgcgcg ataatcttcg
     1861 tcctttttag ttattgccgt aaagtctttt ccgttttgtt tgtaataaaa ctcacgcata
     1921 acttcaagca tgttatgctc aataatggaa acaactgaag aaattccata acgagctacc
     1981 ttgacagggg tgtcaaccgt aaaggcaaga cccataacgg ggatatgaaa acgatgtaaa
     2041 tcagaatgac tcatggattt taattattta tgatataatg ttggggctga ttaatatcaa
     2101 agatatgtga aacacaaatt acgttaatac aattgaagtt accattgata tttgtcattt
     2161 atttttaatt tatcttgaat tgtagattgt tgatgaataa atagttgggt ttataaacct
     2221 gaagaatgaa gttttcaaat aaaagtatca taactcacta tcgtaaggct gaataagaat
     2281 aacagttatg ttttttacaa gggtattagt ttcaacttca gtatggctct gatttaaaaa
     2341 gtttggggtg gcaaaatgca attcagaagg taaattattg tcattttggg agcgtacata
     2401 agccaatgct tcagttatat caggaattag ttgctgtgta tgtgctatga tgactaaatg
     2461 ctgtgttgcc cgaagtaatg ccttttggta aatgttatct tcggataaaa caataagtcc
     2521 attacgtaca accaaacatt cacaaggaat aatcaccacc tgaacatcgg tatcatcttc
     2581 ttcggaaaac acactttgat gtggaaattc aaattcatca aagaaggctg aaaggttacg
     2641 gtcaacacaa acaacctcag taaaatcatt ggcatcgcaa acaccaagta tagtttccat
     2701 tagctctacg cgaccattgc ataaaacaaa ctgaccacct tgttgtgtaa agttttcggc
     2761 aaactgtatt tccggtgaat aggatgtgga attataaaca accttgtcca tatccagatt
     2821 aggatacgga ttaggcgtct ttgcaattaa tgcactacgt acctttttta gaaccttttc
     2881 tctgttggta ctttcgtcca taaattatac ctgaatagat ccttccggaa tatttgatgg
     2941 aggcggactg tcaacagcag gaggaacagt tttctccggt tcaggctcac catctgtaat
     3001 aaatgaattg aaggtggctt tttctgattc gccccatgga cgatgaccaa aaatttcctc
     3061 taaatcttct ttgaaaatca cttctttttc aagcagtctg tgcgctagtt tgtctaattt
     3121 ttctttgtta tcaaccaaca gtttttttgt acgctgatat gctaagtcaa tcagcttttt
     3181 cacttcttca tcaataatct ctgcagtttt ttcactgtat ggcttgctga atgaatagtc
     3241 ttgctgaccg gaagaatcat agtagctgat gtttccgatt ctctcgttta gcccaaatat
     3301 gcttacactg gcataagcct gtttggtaat tttttccaaa tcagaaagtg caccggtgct
     3361 tatggttcca aatatcacct cttctgcagc acgaccacct aaagccgcac atatctcatc
     3421 aaaaatctgc tctttgttgg taagctgcct ttcttcgggc agataccatg ctgcacctaa
     3481 agaattacct cttggcacaa tagtaacttt taccagcgga tgtgcatgtt ccaacaacca
     3541 gcttacggtt gcatgtccgg attcatgata ggctataaca cttttttcgt gtttggtgat
     3601 gattttattt ttcttttcaa gaccaccaat aattctgtca actgcagaaa gaaaatcatc
     3661 ttttccaatt gctgttttat ttttacgtgc agcaataaga gcagcctcat tacaaaggtt
     3721 ggcaatatcg gcaccggaga atcctggagt ctggcgggca agaaatttta tatcaacggt
     3781 gtgatcaatt ttcagtgttt ttgtatgcac tttaaaaatc tcttcgcgtc ctgtaaggtc
     3841 aggaagttct acatatatct gtctgtcaaa acgacccggg cgaagcaatg ctctatctaa
     3901 aatatcggca cggttggttg cagcaagaat aataactccg gtattgctgc caaaaccatc
     3961 catctccgtc aataattggt tgagtgtact ttcgcgttcg tcattagcac tgaaagatgg
     4021 ggctttgcct cgggcgcgac ctattgcatc aatctcatca ataaaaatta tacttggtgc
     4081 tttttctttt gcctgacgga ataaatcgcg cacacgcgat gcgcctacac ccacaaacat
     4141 ctctacaaag tctgatcctg aaagtgaaaa gaaaggcact tgtgcttcac cggcaacagc
     4201 ttttgccaat aacgttttac cggttcccgg agggccaacc aacaaggcac ctcttggaat
     4261 tttacctcca agttgtgtgt attttttagg atttttaagg aagtcaacaa tctccattac
     4321 ttccaccttg gcttcttcaa gcccggcaac atcattaaac gtgactttta cattcaagtc
     4381 tttatcaaat agctgtgcct tagattttcc gatgttgaaa atctgactgc cggcaccacc
     4441 gccacccatt ctgcgcatga taaaaatcca gattacggca atgatgataa acggcaataa
     4501 attccaaaat aacacttcaa gaatagagcg gtcgttgttg ttgtattcag gttgtattct
     4561 ttcattctca gggaagtctt tctggatggc ttctaatttt gattcaaagt tgccgacatc
     4621 gccaatgggc atgtagtaat gcgggccggg gttgtagcca ttgccaaaag gtttgtgact
     4681 aacagcttta tatttctcct ggtttagctt gtctttttta atggttatct ctacacgttc
     4741 gcggttaacg atttcaattt tttctacatc atgagtggct agcatggttt tttcaaactc
     4801 attcatgttg gtcagtttgg gtttatcgcc aagacctaat acccaaatga caaaaatcaa
     4861 caggaataca atggcataaa tccatgaaaa attaaatccc ttcccgggag gttttggcat
     4921 tttgccaaat ggtgatttct tcttgtctcc gcttttattc tcttctgaca tcttttttat
     4981 tcaatcttta gtttttcttg taatcaacaa tcatgcaaac ggcaaaatgg cagttttgtt
     5041 tattattggg ctgcaaattt taccaagggt gcgtctgccc acagcttttc gagcgaataa
     5101 aagtctcttt tatctgccaa aaatacatgt actatcacat taaagtaatc caatagtatc
     5161 cattctgcat tctgcttccc ttcaatgtgt gctgccgttt cttttaactt tttctctact
     5221 ttttcttcta tatttctaac tattgcctca acctgtgttt tgtttgatgc ctgacagatg
     5281 ataaaataat ctgccatagc attatttaat tccttcagat ttaggacaac gatatgctct
     5341 ccttttttat cctctatact gtcaacaatt acggtgagta atttctctgt attatctttt
     5401 aatttacgct tgggttgcct tgccttggtt ttttttagcg tggctgcttt cacaggcgta
     5461 gtttcatctt tatcctttgc aggattcgtt ctttttgcca aattgtaaaa attattgatt
     5521 tgagtcagat acaaaagtaa gaaaaataag gaaagtaact atgtttgctg tcatgtttaa
     5581 aaattctatc tggtttaatc tccatgagac tgagtccaca aacagttttt tacatgaaaa
     5641 gctcatgtca gaaaagctgc ccgaaggctc tgttgtcact gctgattatc agaccaaagg
     5701 gcgtggacat cggggtagta gctggcatag tgaggccggc aaaaacctgc tgatgagtat
     5761 tgtagtttat ccggagtttt taaaggcaac tgaggctttt atgttgagta aatgtatggc
     5821 tctggcaaca tacgatttgt tggccgatta ttcaaataag gtgcagatta aatggccaaa
     5881 tgatatcctg attgaaggta aaaaagttgc aggaattctt attgaaaata ttttacgtgg
     5941 ttcagaaatt tatgcatcaa ttatcggagt cggaattaat ttcaatcaac aattgtttga
     6001 gaaggagcta actacagcta cttctgtatt tatggattcc ggtgttaagg ctgatactca
     6061 gattaacgct gtgaaattgc atgaaaatat tgaggtgtat tatcagaagt tgcaacaaaa
     6121 aaagtttgct gaaattgatc agttatatct aaccaatctt tataagctga atgagtcagc
     6181 attgtttaaa gatgcagacg ggcaatttca gggtaaaata agagggttgt catcaatggg
     6241 tttgttgatt gttgaaagag aaaatggaga aaagcatttg tacgatgtca aagaaatctc
     6301 tttagttcgt taatgtgaaa atcaggaact taatttaata ccataactaa agtgcctgca
     6361 ataatcagac ttgcaccaat tgcagttttt attgttaatg tttcgcctaa aaatatcatt
     6421 gccagaataa ttgtaagggc aatactcaat ttgtcaaccg gtgcaacctg cgacacattg
     6481 cctgtttgca aagctttaaa ataaaacaac cacgataagc ctgtagcaat accggagaga
     6541 atcagaaaaa tgatgttttg ttttgaaagg gtgtttattc cttttgtttc acccttcagt
     6601 aaaattatta tccaaatcat tactaaaaca acaattgttc ttatgcctgt tgcaagattt
     6661 gaattgatgt ttgccacacc taattttgca aatattgctg taagagaagc aaacaatgca
     6721 gaaagtattg cataaatcca ccacatagta tttaaatttt tgtgagtttt aaaaatgctt
     6781 gtagctattt aaagcaaagt aataataaat tgccgattat gtcaatcaat atcagaggtg
     6841 aattctgaaa attttaccgt tatcagaagt ggcaatagca atagtgtcgt ttaaaaattt
     6901 aatagcattt attctggagt tattcaggct gtagcccgaa tcatagtttt cgcctccatc
     6961 aatacttatt gctgtaaatc cactcaatcc gcttgtaata attttgtttt tacgcgcatc
     7021 aatgcataaa aaatgctctc tttttccaaa tgtttgattc tttttatctg ttaaactaaa
     7081 tgtatttccg ccatcacttg tatggtataa agcaccattg taactacagg caactccttc
     7141 tgatgaattg ctaaaagcaa aacccgtata gaaagatctg tcacaatcca aggttgcgaa
     7201 ttcaaagttt tgtttgctta aaagcattgt gccatatcca ccggatagaa tgcctgctgc
     7261 agtaggagtg atacaccgaa gttcatgttc aaagccggca acattccaat gctcaccgcc
     7321 atcagtagtt gaataaataa tgccacggtc aaaatttttt cctccggcaa aaaatcctgt
     7381 gtctttgttt acaaaatatg cactgtgcag cggggagttg tattgaaaag gtattccttc
     7441 ccagtcaaaa tgcaattgcc aattgatgcc gccatcatta gtttgataaa catgcgtgga
     7501 atctcctccg caaaatccat gttgattgtc tataaaaaat atgctgtgca ggttatattc
     7561 aaattggtta ttcaatattt gccacgacag tccaccatct gacgaacgaa gtatgatgcc
     7621 attgcctgtt tgtccgccac agacaaatgc aatgctatcg tttaaaataa aaagattgtt
     7681 aagattaaaa tttgttggtg tagatatttc ctcaaagact aaatctaatt tttctttttt
     7741 gcatgataga aataaagtta gcagcagtac taattttact gcatgacttt tgtgaatcat
     7801 taaaacgaaa aaagaaacat aagaaaaata gcaagccaac tgaagtttac aaaatggctg
     7861 aacaccattg cgctgtttag ttttagtaat tgcccctgat cgctgaaaaa taatagtgaa
     7921 tcaataacat catgcgactg atgaaaagca ttgtaggtga taaaaataag gtatgcaacc
     7981 gttgctaatg catgaaccat gtgcaatgca ataatgccga aaaaataatg ggaattaaaa
     8041 ggcatattgg tatagtatcc tgttttttgc atggcatgta atccgataat ctgagtcaca
     8101 agaaacagtg atgtgatgat ggttgttaaa atggttgctc ttaataattt atcgaacgaa
     8161 tcttttctga aagcactaac ggctttagaa atagtgtagc ttccaaacaa taataggatt
     8221 gtacttagtg taaaaacttt aggcaatgca ggcggcatat tgttgttttc gttttgtgtt
     8281 atcaaaaaca aaaatgataa tgttagaaat aacaatgaac ttatcagtat gccgaaaaac
     8341 aatattgttc tgtaaggatg tattctttca atccttgaaa ctctgattga tctttctttg
     8401 gaatcggttt tattcgttga cataaaagca cctctttaac tcctttcaaa ggtacaacaa
     8461 tcaaacttaa aacaaaaatt atttttttaa cgtccgggtt taaatgtcgg gaatattctt
     8521 ctcaggtatt gaatttttcc ttcaccggcc tttatcggaa atatgccttg ttctacagag
     8581 cgtacatctt caacagtaaa aaacgaactg ccatgatgtt cattgacaat ctctaatatt
     8641 gtagcaatat tttttctttt agctacaatc aataaaattt ttaccggccc ctgcgaaccc
     8701 attccatcaa tctcagttac tcccagattt tgcgcacgta gggcttctaa tagcttttgg
     8761 ctttgcctgc ttgtaataac cctgataatt tgtgtaccta atgccaatcg tttttcaata
     8821 ctgattccca ctaaaattcc cattgaatag cctccggcaa atgcaatgta gcacattata
     8881 ttatgcaggt ttttaataat tgaacttact gccagcagcc agatgagtac ttctacaaat
     8941 ccaattacgg gaataatttt tcttacccct cttgaaagaa atatgttgcg caatgttgct
     9001 aatgttacat cacacatcct gcaaaggaaa atcaataccg gaagaacgac ccaactgtat
     9061 aggtcggttt gcataaacgc ttcgctaaac atttttaatt aatttgttta caagtgcagg
     9121 cacgccctct cctgctgtat tctgaattac tgttagattg tgaatatgat tcaactcccg
     9181 ggctttgggg tcaataatat atttttcgca ggtttgtgat gtgtaatcaa taagacctgc
     9241 tgcaggataa acttccagcg atgttcctat tactaaaaga atgtctgcct tcatggtctg
     9301 aaatatggct tcatccattt taggtaccat ctcaccaaac caaactatgt gcggacgaag
     9361 tcggtgaccg tgctcgttag tgctatccca attcatttcc catccattgg attgataaat
     9421 cattgttgga tcttcttcac tgcgcatttt taaaatttct ccatgcaaat gaatgatatg
     9481 ttttgagccg gcacgctcat gcaggtcgtc cacattttgt gtaattacca caacctgata
     9541 gtgtttttcc aagtcaacta aagccaaatg tgctttgttg ggttgtgcct ccatcacctg
     9601 cctgcgtctt tgattgtaga attctgttac tagctttgga ttgcgctgcc atgcctgcgg
     9661 tgtagccaca tcttcaatgc gatgttcttc ccacaaacca ttgctgtcgc gaaaggtttt
     9721 tataccactt tcagcactga tgcctgcacc tgttaatact acaattcgtt tcattttctg
     9781 ccttgcaaaa ttggtatttc agccggcaca aaaattaaga gcaggattta ttttttaaca
     9841 gtgtaatcgt taatggaaaa tcccctcaga aaatcttcca cattcattct ttttttgcct
     9901 tcaagctgca actctttaat tgaaattaca ccatcagagg tggctaagtt taaaaaactc
     9961 ttgttgtcag aaattatttt acccgcctcc aataattctt tgttttctac aactgatgct
    10021 atatagattt ttaaagtaag tgtttttccg ttcttatctt caagcaaagt aaaagcaccg
    10081 ggatagggcg acaggccgcg tatctgatta aaaactgttg aagttttatc cgaccaattt
    10141 attatacagt cgtttttaaa aattttaggg gcatgtttta ttgttgatgc tatgctgttt
    10201 tgtggcgtag tggaaagatt tcctttttct atttgctgaa cggttttcag aaccaaggct
    10261 gcaccaatgt gcataagtct gtcatgcagt tcgcctgcta tttcagtagt tccaatatca
    10321 actttatcag aaagtataat gtcaccggta tcaatttcat gtttcagaaa aaaagtagtt
    10381 acgcctgttg tggtttcacc attcatgatg gcgcggttta taggtgctgc acctctgtat
    10441 tgtggtagca aagatgcatg aaggtttaac gtccctaagc gtggcatact ccacagttgt
    10501 tccggcatca tcctgaatgc aactactata tgcaaatcgg cttttaagga tgccacctgc
    10561 gaaagaaatg ctgcatcttt caatttttca ggttgaagta tgagtaagcc ttttgactgt
    10621 gcatattttt ttactgccga ctgttgcatt tgcatgccac gacctgcagg tttatcgggt
    10681 gcagttatca caccacaaat attataaccg ttttcaaata aaattttcag tgattcaaca
    10741 gcaaaatcag gtgttccgga aaaaagtatt tttaagtcat tcattgatgc taagataact
    10801 attttgcttt tcggcaatat aagagggtta tggtttgtcc tgccatacag gtagtcatga
    10861 cctgcgtttt tttatagctg atttcaagtt ttaaattatt tttttgaaat gaagcatcca
    10921 gattatcggg tatcaacatt ttctgatcca cagtttttaa aagaaaacca catccatcta
    10981 agtttctcat gtcagtcact gttactgttt caagattttg atttacggtt tcctgctttg
    11041 cagatttgca ggaagtcagc atgtaaataa gtaatagcaa caaaacgtgt tttattttct
    11101 ccatttattt gttcgtaaat atagccatga acataatccc ataattaccc aatatagaat
    11161 ttctacatac cacactacat gaagctgtgc ttttaacact acggcagcga taaaagcata
    11221 agtaatgtaa atagcaatgt ttattacttc tattataaat gctacgttag ttttacctgt
    11281 accactgatg gcactgagca gcaccatact gacactgaaa aaaaacatgg cacaccaggt
    11341 aacgatgtag gaaggtgttg catcatgcaa caacatcata tcagaagaaa ttatgcgcag
    11401 caataatttg aaatcaaaaa gattgataag taagattatg gttgaagaag ctaaactcag
    11461 cactataatt ttttttatca gtggaattac ttcatcttgc ttgttttgtc ctattaggtt
    11521 actcaccatg ctgttggcag cagaagcata gccccagatg ggtgtcatca aaaccatgta
    11581 ggcgctgcgc acaacgtttg aaacggctaa ctcatgctgg ccaagccttt ctataaatac
    11641 aaagaataaa aaccaagcgc ccatagatag caggttctgg aaaaaaacag gtattgaaat
    11701 aaaaataatc tcgtttatgc gtgtcatgtt tatattatca aagcgaaaca gtccaaagct
    11761 gctgttctct ttatgataaa atgtgataaa gaaaagatat atacatgcca gcacttctgc
    11821 aatggcagat gcacgtgcag caccggctat accctgagcc tcgaaaccca attttccaaa
    11881 gatgagcaca taatcgagaa taaagtttga tactgtaagc actaaggcac tgtaagtaat
    11941 aaatcgtgtt gcactaatgc tggtatagta tgcccgaaag caattgacta aaagaataaa
    12001 aaatattcca aaacttctgt tgtgtatgta ggttattgaa gcatcggcaa catcttttga
    12061 actcaacacc agattaaaaa atgcaggagc agcaaaaaac ataatgaaga acaataagac
    12121 agcaagcact aataaaatca caacgctgtg gtcaaaataa tttccgatag caagcctgtc
    12181 gttttctcct gcttttcgtg acatcagaat ctgactgcct attgacaagg ccatgcctag
    12241 catgactaaa acaaaataaa atactccggc aatggcagaa gcgccaagct ccacttcacc
    12301 aacacgtgca agaaatgcag tatctgttaa cccgacaagc atttgtgcta ccgatgccag
    12361 cataatgggg taggcaatct gccagatgtt gaggtatgtg gattttacct gcattttaat
    12421 gtcgaagtct tgtgaaattg atagtgcaaa aataaagggt gggcgaaggt aagaaagagt
    12481 tgtggagtgg ttgaagcata aaaattcaaa aacttattgg aatctgcttt ttacatattt
    12541 ttgaagagat atgtatatgt gaaggttagt a
//
LOCUS       DAOOOL010000077        11370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_519_length_11370_cov_170.833760, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000077 DAOOOL010000000
VERSION     DAOOOL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_11370_cov_170.833760"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..432)
                     /locus_tag="PKU82_07515"
     CDS             complement(223..432)
                     /locus_tag="PKU82_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOZ82756.1"
                     /translation="MSKYEIIIYWSKDDKSYIAEVPELAGCMADGKSYQQALSNVEVI
                     INEWLETAKQLNRTIPKPKGKLMYA"
     gene            complement(429..680)
                     /locus_tag="PKU82_07520"
     CDS             complement(429..680)
                     /locus_tag="PKU82_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015191801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOZ82757.1"
                     /translation="MSKYEKLLFKLLSGKNDTNFAFSDLCKLLDELGFNKRIKGSHHI
                     YYKNNVDEIINIQPDKAKAKPYQVRQVRNLIIKYKLIDI"
     gene            complement(875..2317)
                     /locus_tag="PKU82_07525"
     CDS             complement(875..2317)
                     /locus_tag="PKU82_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOZ82758.1"
                     /translation="MKLPFKKLTTLVLGSFIGPFIMTFFIALFVLLMQFLWKYIDDLV
                     GKGLDWWTITKLLGYTSTTLVPLALPLAILLSSIMTFGNLAEHYEIVATKSAGISLQR
                     LMRPLVVVAIIISLGAFYFSNNILPYSNLKMGSLLYDVRQQKPALFIKEGVFYNGIDG
                     YSIKIGKKADDGKSIKNIMIYDHTHNMGNKKVVIADSGTMVMTNDKRYLILTLINGHS
                     YEEQQNRKKNYDTNPLLRTQFQSETIRFDLSSFQLNRTNEDLFKDNYQMMNVKQLNAM
                     IDTFQLQAAQRKTRFARDIKPYYQFLKDSTLFIHVSNAIAKPFLTDSIKLNNTKFETA
                     LSQCRVIKSYIESADDDLESRNRAYVKYQIEWHRKFTLSIACLLLFLIGAPLGAIIRK
                     GGLGLPIVISIAFFLCYHIISITGEKMAKEGIISVERGMWQSSLVLLPIGIFMVYKAT
                     HDSALFDMDSYKSFFKKLFRKKRLDDFLPK"
     gene            complement(2337..2810)
                     /locus_tag="PKU82_07530"
     CDS             complement(2337..2810)
                     /locus_tag="PKU82_07530"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOZ82759.1"
                     /translation="MSNHNHFMEEAVALSKKAITDNEGGPFGCVVVKDGKIIAKGNNK
                     VTSTNDPTAHAEVVAIREACKALNSFQLDGCEIYTSCEPCPMCLGAIYWARPDVVYFA
                     NTRQEAAAIGFDDEFIYDEINVHPTERKIKMVHLPNLQAAGVFNSWQNKSDKTEY"
     gene            complement(2814..3218)
                     /locus_tag="PKU82_07535"
     CDS             complement(2814..3218)
                     /locus_tag="PKU82_07535"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ82760.1"
                     /translation="MPTSEVTYIGELRVKSVHVFSGNEIITDAPLDNQGKAQAFSPTD
                     LLATSLANCMLTIMGIAARTHQFSIDGTRAEVTKTMAADPRRVSAIEVKLFFPKNNFS
                     DKEKKMIENAAHTCPVAYSLHPDIKQDISFNF"
     gene            complement(3221..4879)
                     /gene="recN"
                     /locus_tag="PKU82_07540"
     CDS             complement(3221..4879)
                     /gene="recN"
                     /locus_tag="PKU82_07540"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOZ82761.1"
                     /translation="MLTRLAIKNYVLIRELDINFSDGLSIITGETGAGKSILLGAMGL
                     VLGERADAKTLFDKKGKCIIEAEFSISHYNLNDFFSQHDIDFESNTIIRREINSEGKS
                     RAFVNDTPVNLSVLKELSSYLVDVHSQHQTLTLNTSAFQLNVLDITAGNQKQLNDYSN
                     QFNEYKKLLKQLVELKTLESKSIAESEFLQFQFDELESAGLKQGEFEKIESELQLLNN
                     TENIVLTLDSTSDILSDGETNVLSNITQAVTRLNGIARLHPSLEDILKRIQSCNIELK
                     DISSELTALKSQFSLNPSQLEILNDRLSVYNKLQQKHRVKNADDLIVLQQEIANRLQT
                     FQSLTDDIEKCDKQTQDLYNKLKQIAAELHSSRQKAAQSFKKDILKLLKEVSLNDAQF
                     EVDLTATEELTATGTTAVRFLFSANKGVTVMELAKVASGGELSRLMLCIKALIANKTS
                     MPTIIFDEIDTGISGEVAHKVGNLIEQLSESRQVLAITHLPQMAGKGKTHYFVFKHIE
                     KGIAQSRIKTLSAEERVVEIAKMLSGEEVTDASLQNARHLLIKS"
     gene            complement(4913..6130)
                     /locus_tag="PKU82_07545"
     CDS             complement(4913..6130)
                     /locus_tag="PKU82_07545"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOZ82762.1"
                     /translation="MTPDNTMKLNSIEEAIADLKNGKAVIVVDDADRENEGDFITAAH
                     NATPEMINFMASHGRGLICATLTEQRCEELALSMMVATNTSSHETPFTVSVDLLGHGC
                     TTGISASDRSKTIKALIDPATKPSDLGRPGHIFPLKSKNGGVLRRAGHTEAGVDLARL
                     AGFEPAGVLVEIMNEDGTMARLPDLVKIAKKFDLKIISIEDLISYRLKTESLIDREVD
                     VKLPTAYGSFELFAYKQNTTGQEHLALVKGEWKKDEPILVRVHSSCLTGDIFGSCRCD
                     CGPQLHKAMQMIEKEGKGVIVYMNQEGRGIGLLNKLKAYKLQEAGKDTVEANQELGFK
                     PDERDYGVGAQILRDLGVSKLKLMTNNPTKRAGLIGYGLEIVEIIPLEIESNEHNRHY
                     LQTKRDKMGHTIK"
     gene            6688..7113
                     /locus_tag="PKU82_07550"
     CDS             6688..7113
                     /locus_tag="PKU82_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82763.1"
                     /translation="MKKLSLSKTLLTGAVVALMAAGFTACKKDSDPDRKKFLGSFSVT
                     TGTISCGVSGNGTIAAGTAVVITENSGGDEKVNVSVGGQLSVVATANGNTITLDNQTV
                     SGFTYTGTGTLSSNTLTLSINEYDATVPENCVYSMTANK"
     gene            complement(7246..7656)
                     /locus_tag="PKU82_07555"
     CDS             complement(7246..7656)
                     /locus_tag="PKU82_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82764.1"
                     /translation="MLRGGEKYFIIGNFNNDANTTLDSNNQANLFYAGSYYYIDDVSV
                     VDCTVGINEIESYKNKIKLMPNPAKDELRIMSDESGIKKIEVCDVLNNVMLAESAEAK
                     EKSITINVSNFASGTYFIKVFSNKGIAVKKFVKE"
     gene            complement(7619..8194)
                     /locus_tag="PKU82_07560"
     CDS             complement(7619..8194)
                     /locus_tag="PKU82_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82765.1"
                     /translation="MRIKLLILFVFISLLGFAQNLVINPSFEDTISCISWPGWPQMQC
                     SYWIWGNGGSPDYFNLNYCGGANVPNNQFGYQFAKTGAAYCGLALYLTPMVQPNGREY
                     LGGQLNDTLKQGHTYCVSFYVAKADSSWYYTSSIGMYLSQNSSVDYSTALNLPYTPQI
                     VNTNGIIYDTLNWTQISGTYVAGGGKIFYHW"
     gene            complement(8273..8911)
                     /locus_tag="PKU82_07565"
     CDS             complement(8273..8911)
                     /locus_tag="PKU82_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ82766.1"
                     /translation="MAEKLIILEPDKYYHIYNHAVGKENFFESDSDFNVFLEKFIKYA
                     SPICDVLTYCLMPNHLHFLVRIKNELRVMTIVKKPFLKNYSHNIVSISKNDILTDIIS
                     KQFSHLFNSYAKYYNYTHHRKGTLFSRAFRRKLIDSDDYLKQLICYIHQNPVNAGLCR
                     KPDEWKYSSYNALVNLKDTLVCRNEVIALFDDVDNFKFCNQVKVDIKLDFQE"
     gene            complement(9031..10359)
                     /locus_tag="PKU82_07570"
     CDS             complement(9031..10359)
                     /locus_tag="PKU82_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82767.1"
                     /translation="MKKLFCFLLLYQIGYGQVWKPLGHRFLYPGVFDFRNFNNQLLVA
                     GDYTADSSTLNNIARWDGSDWQPLDSGMLGGVYAMEEFNGELHIGGYFYNINNILNTR
                     KIARWDGVTFHPLAKGITSGGQVEVMLSYNGDLYVGGSFQTVDSLPIHTIAKWDGSAW
                     SPVCNITGTFAEVESMAIYNGDLYIGGYFTGINGQPMRNIARFDGISWSAVGDGLNSD
                     VYALVVDSATNRLYAAGNFTSTGTGAIIFPSNVAYWDGAVWTPVGSGPSLSPTSLTIY
                     RNQIYVGGYFSWINNIINSSGDTLNFICRWDGNDWQPLGKGLNCSVQALSVFNNDLIV
                     GGCFTMAGDSVVNLVAAYTDTTTSVQELNTVKQEITITPNPTKNSIAVTSPVAVTAYR
                     ITSPTGQIIKKQNQKPLKEFSVDTSALPNGLYIFTATMGKQLVSIKFTKE"
     gene            complement(10546..>11370)
                     /locus_tag="PKU82_07575"
     CDS             complement(10546..>11370)
                     /locus_tag="PKU82_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82768.1"
                     /translation="NISGTFPRVSAMVVYNNELYVGGYFEFVNGISMVNIAKYNGTIW
                     SDVDMGVNSNVIAMVVDSVNNRLYAAGNFTGCIGSGMLFPTNVAYWDGMTWHGVGTNP
                     SINPRALCFYKNSLYAGFSFEAIKANNDTLKYIAKWDGNEWQSVNKGVNDNVIELKVL
                     NGMFIVAGSFTQAGDSSVNYVAAYTDTTTSVHELNTVKQEITITPNPTKNSISVTSPV
                     AVSAYSITSPTGQIIKKQNQKPLKEFSVDTSTLPNGLYIFTASMGKQLVSIKFAKE"
BASE COUNT         3386 a         2230 c         1968 g         3786 t
ORIGIN      
        1 gtaatttgaa ctccaatttc ttattcctta acttaatgac attgggcgca agtctgaaga
       61 cttgcaccag aggggaatat ctgtaggtgc gaggctgtgc ctcgttgcct attattttaa
      121 tggcttgcag ccataaaatt aatctctctt tcgctaaaag cgaaaataat aaaatgacac
      181 gagcgacacg ctcgcgctta caaagatggg cggcaggaaa ggtcaggcat acatcagttt
      241 tcctttgggt tttggaatgg tgcggtttaa ctgcttggct gtttccaacc actcgttaat
      301 aatcacttcc acatttgaca atgcctgttg atatgatttt ccgtcagcca tgcatcctgc
      361 cagttcaggt acttcggcaa tgtagctttt atcatcctta ctccaataaa taatgatttc
      421 atatttactc atatatcaat cagtttatat tttataatca gatttcttac ttgtctaacc
      481 tgataaggtt ttgctttcgc tttatcaggt tgaatattta ttatttcatc aacattattt
      541 ttataataaa tatgatggct gcctttaatt cttttattaa atcctaattc atccaacagt
      601 ttacataaat cgctaaaagc aaaattagtg tcattttttc cactcaacaa tttaaataac
      661 aacttttcat atttactcat aggcaatcaa gacaaaatag aaagagcaaa tatacaatta
      721 ttgatttcac tagacaaatc agcgtagcct ctgttctcag taggtgcgag gctgtgcctc
      781 gttacctatt attttaatgg cttgcagcca taaaattaat ctctctttcg ctaaaagcga
      841 aaatagttaa atgacacgag cgacacgctc gcacctactt aggtaaaaag tcatcaagcc
      901 gttttttccg gaacagtttt ttgaaaaatg acttataaga gtccatatca aacaaggctg
      961 agtcatgtgt ggctttgtaa accataaaaa ttccgatagg cagcaacacc aatgacgatt
     1021 gccacatgcc tctttctaca ctgatgattc cttcttttgc catcttttct cctgtgatgg
     1081 aaataatgtg atagcagagg aagaatgcaa tggaaattac aataggtagt ccaagaccgc
     1141 ctttgcggat gatggcacct aaaggtgctc caattaagaa cagcaataaa caggcaattg
     1201 ataaagtgaa tttgcgatgc cactcaattt gatattttac ataggcacga tttctgctct
     1261 ctaaatcatc atcagcactt tctatatacg atttgatgac acgacattgc gacaaagctg
     1321 tttcaaattt ggtgttgttt agtttgatgc tatcagttag aaaaggtttt gcaatggcat
     1381 tgctaacatg gataaacaat gttgagtcct ttaaaaactg ataatatggt tttatgtctc
     1441 tggcaaaacg tgtttttcgc tgtgctgcct gtaattgaaa agtatcaatc atggcattaa
     1501 gctgtttgac attcatcatc tgatagttgt ctttaaacag gtcttcattg gttctgttta
     1561 gctgaaaaga cgataagtca aaacgaatag tttcggattg gaattgcgtt ctgagtagtg
     1621 gattggtgtc gtaatttttt ttacggtttt gttgttcttc gtatgaatgc ccatttatta
     1681 aagttagaat aagataacgc ttatcatttg tcatcaccat agtgccacta tcggcaatca
     1741 caactttttt attacccatg ttgtgtgtat gatcataaat catgatgttt ttgattgatt
     1801 taccatcatc agctttttta cctattttaa tactgtagcc atcaatgcca ttgtaaaaaa
     1861 caccttcttt aataaacaat gccggctttt gttgacgcac atcataaagc agcgagccca
     1921 ttttaagatt actgtaaggt aaaatgttgt ttgaaaaata aaaggcgcct aaagaaatta
     1981 ttattgcaac cactactaat gggcgcatta acctttgtaa agaaatacct gccgattttg
     2041 tggcaacaat ctcatagtgt tccgcaaggt tgccaaaagt cattattgat gacaacaata
     2101 tagctaatgg caatgccaaa ggaactaagg tggttgatgt gtagccaaga agtttagtga
     2161 tggtccacca gtcgagtcct ttgccaacta aatcatcaat atacttccac aagaattgca
     2221 tcagcagcac aaagagtgct ataaaaaatg tcatgataaa aggaccgata aaagaaccta
     2281 atacaagtgt ggtgagtttc ttaaatggta atttcatcaa ttatctgttg ctgatatcaa
     2341 tactcggttt tgtcagattt attttgccat gaattaaaaa caccggcagc ctgcaggttg
     2401 ggtaaatgca ccatttttat ttttctctcg gtggggtgaa catttatttc atcataaata
     2461 aattcatcat caaaacctat tgcagcagct tcctgtctgg tattggcgaa gtaaacaaca
     2521 tccggtcgtg cccaataaat agctccaagg cacatagggc aaggctcgca ggaagtataa
     2581 atttcgcagc catctaactg aaaactgttg agtgctttac aagcctcgcg gatggcaact
     2641 acttctgcat gtgcggtagg gtcgttggta gaggtaactt tattatttcc tttagcaata
     2701 atttttccgt ctttaacaac aacacagcca aaagggccgc cttcattgtc agtgattgct
     2761 ttcttggaaa gggcaacagc ctcttccata aaatgattgt gattgctcat ttattaaaaa
     2821 ttaaaactga tatcttgttt gatatcggga tggagactgt atgcaacagg acaggtgtgg
     2881 gctgcatttt ctatcatttt cttttcctta tcagaaaaat tatttttcgg aaaaaacaac
     2941 ttcacttcaa tagcgctcac tcttctaggg tcagcggcca tagtttttgt tacttctgct
     3001 cgtgtgccat caattgaaaa ctgatgtgtt cttgcagcga tgcccataat agtgagcatg
     3061 cagttggcta gtgatgttgc taaaaggtcg gtgggggaga aggcctgtgc ttttccctga
     3121 ttgtcgagag gcgcatcggt gatgatttca tttcctgaaa aaacatgaac cgattttact
     3181 cttaattcgc ctatataggt aacttctgat gtgggcataa ttatgatttt ataagtagat
     3241 gtcgtgcatt ttggaggctt gcatctgtaa cctcttcacc gcttaacatt tttgcaattt
     3301 caacaactct ttcttcagca gaaagggttt ttattcttga ttgtgcaatt cctttttcaa
     3361 tatgtttaaa aacaaagtaa tgcgtttttc ctttgcccgc catttgcggt aggtgtgtta
     3421 tggcaagtac ttgtctgctt tctgaaagtt gttcaataag gttaccaact ttatgggcta
     3481 cctcaccgga gatgcctgtg tcaatctcat caaagatgat ggttggcatt gatgttttgt
     3541 tggctataag tgctttaata cataacatca gtcgtgaaag ttcaccaccg gaagcaacct
     3601 tcgccaattc cattaccgtt actcctttgt ttgcagaaaa taaaaagcgc actgcagttg
     3661 tgcccgttgc tgttaactct tctgtggctg tcaggtcaac ttcaaactga gcatcgttta
     3721 acgaaacttc tttcagtaat ttcaaaatat cttttttgaa actttgtgca gctttctgtc
     3781 tgctgctatg gagttctgcc gcaatttgtt ttagtttgtt atataaatcc tgagtttgtt
     3841 tatcgcattt ctcaatatcg tcagtaagcg attgaaaagt ttgaagtctg ttggcaattt
     3901 cctgctgcaa gactatcagg tcatcagcat tttttactct gtgtttttgt tgtagcttgt
     3961 tataaacaga aagtctgtca ttgagtattt caagctgtga tgggtttaaa ctaaactggc
     4021 ttttaagtgc agttaattcc gatgaaatat ctttcagttc aatgttgcac gactgaattc
     4081 ttttcagaat gtcttcaagc gatggatgta atcgtgcaat tccatttaaa cgggttacag
     4141 cttgtgtaat gtttgaaagc acatttgtct cgccatcaga caaaatatca cttgtgctgt
     4201 caagtgttaa tacaatattt tcagtattgt taagtagttg cagttctgat tcaatttttt
     4261 caaactcacc ttgctttaat cctgccgatt caagttcgtc aaactgaaac tgtaaaaact
     4321 cagattctgc aatactctta ctctccaatg ttttcagttc tacaagttgt ttcaataatt
     4381 ttttatattc attgaattgg ttgctgtaat catttaactg cttctgattg cctgctgtaa
     4441 tgtctaatac atttaactga aatgccgaag tatttaatgt caatgtctga tgttgcgaat
     4501 gaacatcaac caagtagcta ctgagttctt tcagaacaga aaggtttacg ggagtgtcgt
     4561 taacaaatgc acgcgatttt ccttctgaat taatttctct tctgatgatg gtgttggatt
     4621 caaaatctat atcatgctgc gaaaaaaaat cattcaggtt gtaatgagaa attgaaaatt
     4681 ctgcttcaat aatacattta ccttttttat cgaaaagtgt ttttgcatca gcacgttctc
     4741 ctaaaacaag ccccattgca cccagcagaa tagattttcc tgcacctgtc tcgcctgtga
     4801 taatacttaa cccatcagaa aaattaatat ccaattctct gataagcaca taatttttta
     4861 tggcaagacg ggttaacata caatgttgtt tcttaaaata atatcctgtt gattatttaa
     4921 ttgtgtgtcc cattttatcg cgctttgttt gcagataatg tcggttgtgt tcgttggact
     4981 caatttctaa aggaataatt tcaacaatct ctaagccata gccaatcaat ccggcacgct
     5041 ttgtagggtt gttggtcatg agttttaatt tagaaacacc taaatcgcgt agaatttgtg
     5101 cgccaacacc ataatctctt tcatcaggtt taaaacccag ttcctgattg gcttcaacag
     5161 tatcttttcc tgcttcctgc agtttatatg ctttaagttt attcagcaat ccgatgccac
     5221 gtccttcctg attcatataa acaattacac cctttccttc tttttctatc atctgcatgg
     5281 ctttatgtag ttgcggcccg cagtcgcagc ggcaagaacc aaaaatatca cctgtaaggc
     5341 aggaggagtg aaccctcact aaaataggct cgtctttttt ccattcgcct tttactaaag
     5401 caagatgctc ttgtcctgtt gtgttttgtt tataggcgaa aagctcaaag ctaccatagg
     5461 cagtaggcag tttaacatcc acttcccgat caatgagcga ttctgttttc agccggtagg
     5521 agataaggtc ttcaatagaa attattttca ggtcaaactt ttttgcaatt tttaccaaat
     5581 ctggaagtcg tgccattgtt ccatcttcat tcataatttc tacaagcaca cctgcaggct
     5641 cgaatccggc aagacgggca aggtcaacac ctgcttctgt atgtcctgct cttcttaaaa
     5701 cgccaccatt tttactttta agagggaaaa tatgtccggg gcggccaagg tctgaaggtt
     5761 ttgtggccgg gtcaattaat gctttaatgg ttttagagcg gtcggaggcc gaaattcctg
     5821 tggtgcatcc atgtccaagc aaatcaacag aaacggtaaa tggtgtttcg tgagaggaag
     5881 tatttgttgc caccatcatg ctcaaagcca actcttcgca gcgttgttct gtaagagtag
     5941 cacatataag gcctctgcca tggctggcca taaaatttat catttcagga gtggcattgt
     6001 gggcggcagt aataaagtcg ccctcatttt cgcggtcggc atcatcaact actatcaccg
     6061 ccttgccatt tttaagatcg gctatagctt cttctatact attcaatttc attgtattgt
     6121 caggagtcat tgtctctaaa tctgtttttg caaatttaca acaagctttt caatgtttag
     6181 ataaaattct tacagtagaa taggctggtt ttttgtcatt tttctgtatg ttaagatgaa
     6241 gtgaagattg taataggtat ataatcttac atacgtaatg tacgtttata gtagtgtaac
     6301 aaaaggaata aagcctgcgt taaattgcac ataacgttct tgataaagat tattactaag
     6361 atagtttggt tttaggttaa tattaaaatg attcatcctt agccctgtaa ggctaaaagt
     6421 tgaatcacct aagcaaaaaa tagttttagg ttggtttcag gtgtgggctg ccccgtaaga
     6481 cagcccgaca tctgcatttt aaattcggat ttggttttag ggttatactt ttaaaaatca
     6541 ccgccaacgt ggcggtgatt ttttgctttt aaagggtttt atggttttca catctaaacg
     6601 tgaataactt tcagttaaaa aatagtggct tggtaagata attcatgcta tatttgcccg
     6661 cataaacatt ataaacttta tttaaaaatg aaaaaattaa gcttatcaaa aacacttttg
     6721 acaggtgctg tagtagccct aatggcagca ggatttaccg catgtaaaaa agacagcgat
     6781 cctgatcgta agaaattctt aggttctttc tctgtaacaa caggaacaat ttcttgtggt
     6841 gtttctggaa acggaacaat tgcagcaggt actgctgtag ttatcactga aaattccggt
     6901 ggtgatgaaa aagtaaacgt tagcgttggt ggccaattat cagtagttgc tactgcaaac
     6961 ggtaacacta ttacccttga caaccaaact gtaagtggat ttacttacac cggtacaggt
     7021 acattaagca gcaacacact tacattgtct atcaatgagt atgatgctac agttcctgag
     7081 aattgcgttt atagcatgac tgctaacaaa taattgttta gcataaagta tagaaaaaga
     7141 gggctatgcc ctcttttttt gttttaagac agattgtgtt tttgaaaaac agggtgattt
     7201 agttcaatta atcacaggca ggtgatatac gaattaatga gtacattatt cctttacaaa
     7261 ctttttaact gctatacctt tattactaaa tacttttata aaataagttc cacttgcaaa
     7321 attactcaca tttatcgtaa tacttttttc ttttgcctct gcactttcag ctaacattac
     7381 attgttaagc acatcgcaaa cttcaatctt tttaattcct gattcatcac tcataattct
     7441 taattcatct ttcgctgggt ttggcatcag ctttatttta ttcttatagc tctctatttc
     7501 atttatccca accgtacaat ctactacgct tacatcatct atgtaataat aagagccggc
     7561 ataaaataaa tttgcttgat tattggaatc aagagtagta ttggcatcat tattaaaatt
     7621 accaatgata aaatattttt cccccccccg caacataagt gccgcttatt tgtgtccagt
     7681 ttaaggtatc ataaataatg ccgttagtgt ttactatttg aggagtatat ggtaaattta
     7741 aagccgtgct gtaatcaacc gaactgtttt gcgacaaata cattcctata ctgcttgtgt
     7801 aataccaact gctatctgct tttgccacat aaaaactcac acaataggta tgtccctgct
     7861 ttaaggtatc atttaattgc cctcccaaat attctctccc attaggttga accattgggg
     7921 ttagatataa tgctaatccg caatatgcag ctcccgtttt ggcaaattga taaccaaatt
     7981 gattattcgg aacattagcc cccccgcagt aattgagatt aaaataatca ggacttcccc
     8041 catttcccca aatccagtaa ctgcattgca tttgtggcca gcccggccag cttatacaag
     8101 aaattgtatc ttcaaaactt ggattgataa ccaagttttg tgcaaagcct agcaaagaaa
     8161 taaaaacaaa taatatcagt aattttattc tcatttcaaa attttgattg cacctgacaa
     8221 gtcgagcagc ccctgtcagt gtgatttcaa aagtataaaa aatatactat attcattcct
     8281 gaaaatccaa ttttatatca actttcactt gattacaaaa tttaaaatta tccacatcat
     8341 caaacaaagc gataacttca tttctgcaaa ccaatgtgtc tttcagattc accaatgcat
     8401 tataagaaga atacttccat tcatcaggtt tacgacataa accggcattt acaggatttt
     8461 gatgtatata acaaattaat tgctttaaat aatcatcact atctatcaat tttctacgaa
     8521 atgcccgaga aaacaatgtg ccttttctgt gatgtgtata gttatagtat tttgcgtagg
     8581 aattaaataa atgtgaaaat tgtttactga taatatcagt taaaatatca ttttttgaaa
     8641 tggaaacaat attatgacta tagtttttta agaaaggttt cttgacaata gtcattaccc
     8701 ttaattcatt ttttatcctc accaaaaaat gtaaatgatt tggcataagg caatatgtta
     8761 atacatcaca aataggacta gcatacttaa taaacttttc aagaaataca ttaaagtctg
     8821 agtccgactc aaagaaattt tctttaccaa ctgcatgatt gtagatgtga taatacttat
     8881 ccggttctag aataattaat ttttcagcca ttgtttttca aaaccgaaaa ttaaagattt
     8941 tatgctctta gattgcaatt tgattgaatt agttttcaca ctgacaggag ctacgcgacc
     9001 tgccccttag gttaaaacac aagccatgac ttactctttt gtaaatttta tgctcaccaa
     9061 ctgtttgccc attgttgcag taaaaatgta caaaccatta ggcaatgcag aagtgtcaac
     9121 actaaactcc tttagcggct tttgattttg cttttttatt atttgccctg ttggcgaagt
     9181 aattctgtat gcagtaactg caacaggcga tgtaacggca atactgtttt ttgtcggatt
     9241 gggagtaatt gttatttcct gctttacggt atttaactcc tgtaccgatg tggtggtatc
     9301 cgtataggca gccactaaat ttacaacact gtcacctgcc atagtaaaac acccacccac
     9361 tattaaatcg ttattaaaaa cacttaatgc ctgaacacta caattaagtc ctttgcctaa
     9421 tggctgccag tcatttccat cccatctgca tataaagttt aaagtatctc cggaggaatt
     9481 gattatgttg tttatccatg aaaaatatcc cccaacataa atttgatttc tatatatcgt
     9541 taatgaggta ggggataatg aaggtccgct acccaccggt gtccacactg ccccatccca
     9601 gtaagcaaca ttagatggaa aaataatagc acctgtgccc gtgcttgtaa aatttcccgc
     9661 agcatataac ctgttggttg cgctatctac aaccaatgca tacacatcgc tgttcaatcc
     9721 atcgcccact gccgaccacg atataccgtc aaaccgtgca atattgcgca tgggttgccc
     9781 gtttatgcct gtaaaatagc ccccaatgta caaatcccca ttgtaaatag ccatagattc
     9841 tacttctgca aatgtgcctg ttatattaca taccgggctc catgcgctgc catcccattt
     9901 ggctatggtg tgaatcggta agctgtcaac cgtttgaaat gatcctccaa cgtataaatc
     9961 accattgtag cttagcatta cctctacctg accccctgat gtaattcctt ttgctaacgg
    10021 atgaaatgta actccatccc atctggcaat ttttcgggtg ttcaatatgt tatttatatt
    10081 ataaaagtat ccgccaatat gtaattctcc gttaaattct tccattgcat aaactccccc
    10141 taacatacca ctatctaacg gctgccagtc cgatccatcc catcgtgcaa tattatttag
    10201 ggtggaagaa tccgcagtgt aatcaccggc tacaagtaat tgattattaa aattacggaa
    10261 atcgaaaaca ccaggatata aaaaacggtg gcctaatggc ttccatacct gaccgtagcc
    10321 aatttgataa agcaacaaaa aacagaatag ctttttcata atcataaaac atcccgtccc
    10381 gtaatattac aggacgggat gctaaattaa acgtttattt gattactacc agctttttag
    10441 tcaggcttat attcttgcca aaaatcttgc caaaaatctt gcaaacatat aagccttcat
    10501 caagattttt gagaggaaac acaaaatagt tacaacaagc ctgacttact cttttgcaaa
    10561 ttttatgctc accaactgtt tgcccattga tgcagtaaaa atgtacaaac cattaggcaa
    10621 ggtagaagtg tcaacactaa actcttttag cggtttttga ttttgctttt ttattatctg
    10681 accagttggc gaagtaatgc tatatgccga aacagcaact ggtgatgtta cacttatact
    10741 gttttttgtt ggattgggag taattgttat ttcctgcttt acggtattta actcatgtac
    10801 cgatgtggtg gtatccgtat aggcagccac atagttaacc gatgaatctc ctgcctgagt
    10861 aaaactacct gcgacaataa acataccatt tagtactttt aactcaatta cattatcatt
    10921 tacccctttg tttaccgatt gccattcatt accatcccat ttggctatgt atttaagcgt
    10981 atcattattt gcctttattg cttcaaatga aaaaccagca tacaagctgt ttttataaaa
    11041 gcataaagcg cgaggattaa tagagggatt tgtacccaca ccatgccatg tcataccatc
    11101 ccaatatgcc acattggtag ggaataacat cccacttcca atgcatccag taaaatttcc
    11161 tgctgcgtat aacctgttat ttacactgtc aactaccatt gcaattacat tactatttac
    11221 ccccatgtct acatcactcc atattgtgcc attatatttt gcaatgttta ccattgaaat
    11281 tccatttaca aattcaaaat atcctcccac atacagttca ttattatata ctaccatagc
    11341 agatacacga gggaaagtac cacttatatt
//
LOCUS       DAOOOL010000078        11313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_528_length_11313_cov_99.963315, whole genome shotgun sequence.
ACCESSION   DAOOOL010000078 DAOOOL010000000
VERSION     DAOOOL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_528_length_11313_cov_99.963315"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="PKU82_07580"
     CDS             complement(<1..1884)
                     /locus_tag="PKU82_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ82769.1"
                     /translation="MIKFFKSLLLFLVFTLPALADSLTINLNEFTANDGLSDNQTNCV
                     IQDKYGFIWIGTKDGLNRFDGKSFQVFKFQSDKNSVSGNNITCLEVTDDSLLWIGTAS
                     NGFCSYNYKTNTFQNYHARNSPLRAEYINDLAFDKVRNCIWVAMNNGGLQQFDLKNKK
                     LNPYVYSINSYYAVAVKDTISLFAGIIESLKKLDNVGRNKGMVGDTATTINTIYIDND
                     LNVWCGAWDNALHLFNIHGKRLQSYIFDGTSKFKKSGEEIISISSDASNNIWCGTKSS
                     GIFIFNKASKSFINNINFSHQINTRVNELFRDSQNRIWIATENGLLVYDQHRNQFRTE
                     MLPVPKGVNACKVFDRLITDKGLDLVITQCGLFYKKSPNESYKFKTVIYRNEEQELYS
                     ILMTKNGDIYIGSNRTVFKFNPENISFNTIDFNKNLKETSFYFNGASLVNSLTSFKQD
                     NSELIAASYYGNYIVLIDFDNRNLYYLIHENTKISDNLENLSRKIWVDSKNNFWVCGR
                     SKGLVQLKFKSDFSLSKYAADNDSMLPITVMPTNWNMLPNSIPIKIKNVFDIAERKDG
                     TYWLSTQGAGLVLFSKENKTSPFKIIDSDITSMQGIKSTGDSLLWIITSSGLLQYNEK
                     TNFF"
     gene            complement(2033..5236)
                     /locus_tag="PKU82_07585"
     CDS             complement(2033..5236)
                     /locus_tag="PKU82_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82770.1"
                     /translation="MKRVANLFISLAFLLYVKAASAQLTLAEWNFPNNPDNATVDISI
                     PANSAKTIYTVGGTAAVVYTTAGATTQSAWTTGWDNGSGTKYWETEINTTGYFNLELS
                     SKQRSSPTGPKNFAIEYKIGIAGTWTPIPGAGAIVVADNFTSGVVTDIPLPVACENQA
                     SVFLRWIMTTNTSVNNSTVASGGSSRIDDVIIKANDAQNHYRSITSGAWNSLSTWQQS
                     PDLVTWTAAVFLPNYYAKTITIRSGHTVTISANVRIDETTINNGGTVNYNSKLLTINN
                     GTGVDLQVNGTFTDGSSTSVAWLAGATWALGASGTYVKTENTNATNWQNNYQGGISTI
                     PATASWVLNKTPSSVTAPSLTTLNMFYPNLTIMNTSGGVWTTAVSSTFTGSTATATIK
                     GNFDVGGVLAPLLGTVDFLNDNNFATPVQVQGNILIRGGCNLRNFGTGFEIFGNLTVN
                     GSTSYGAANARKYRFSGGNAQSISGSAALTSLQIYQLEMNKSANSLTLLKSVKVDNNL
                     NLITGIINSSTPNILIVENGATATGASNVSFVNGPVQKNGSQAFTFPVGKNTSYRSIA
                     IGADASSSSFWTENFNTGAGWSLVNVMGPEGADPNPFVISDAEGGVAAGGCGVANNGN
                     NTMHVSSVFNPTGGAAYDAGGLCGLLYCPQTNRMAQSPVINCTGKSNISISFNYIEFG
                     DGTIDNATLWYYDGAAWAQITDMPKTACCGGACNGFRQGKWTAFSMVLPASANNNANV
                     RIGLKWENNDDGVGTDPSFAIDDISLSSLTSSQFVAEYMRANPQITYSNIFDPTLDHI
                     SQCEFWTLNQIAGSSSRNVTLSWDASSCGVTLLSDLRVARWRSVGTIWNNLGNGSTTG
                     NVSAGTITTSTADNLFGPFTLSSASSENPLPVELLNFTAQLNNDQVELRWSTVSEINS
                     DYFEIERAAVDLNFIPINKTQAAGFSNQLLRYHATDSKPLARKSFYRLRQFDFNGESH
                     LSNSVAVELNNYEQLQINYAVFTNKNNLYFKVTGNVEKNLTAEVYDINGAIISTQELN
                     GTENIFHVQTQNLANGIYVLKISNSNSSVTKRFSVHD"
     gene            complement(5499..5801)
                     /locus_tag="PKU82_07590"
     CDS             complement(5499..5801)
                     /locus_tag="PKU82_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82771.1"
                     /translation="MKKGKRKFAFDFVHPAFKIESNSTLLNIFPNPSSGVFNVQSDNQ
                     LENVQITVTDLQGRTLINTLFSGNETTVDLSNYTQGVYLLQLHSSNVVQTYKLIKD"
     gene            complement(6264..8015)
                     /locus_tag="PKU82_07595"
     CDS             complement(6264..8015)
                     /locus_tag="PKU82_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOZ82772.1"
                     /translation="MKKTLLTIFVMATTLLVQAQTPAFEWVQQVGDSAGDQGRSIARD
                     AAGNLYVAGCFGKTVDFDPGPGVYNLTSSVGLELFVLKLDTAGNFEWAVKTTGWIGNL
                     SEVALTCSDAGNVFVTGHFKDSVDFDPGSATFNLTSNGGRDIFIWHLNTNGNFVWAKN
                     IGSTASSINSRDVGYDITTDNWGNVYTIGSFELSADFDPGPGVVTLNAGPYCDIFISK
                     LNATGDFVWAKNFGGFNYCIGNSIAIDALGNVHATGSFSDTVDFDPGAGVFNLIAPRS
                     NQFVASDPDIFILKLDSTGNFIWAQNFGATSEDVGQAISVDANGNVFTTGYFEDAVDF
                     EWGNGTFNLTTSIFEDVFVLKSDAAGNFLWAKQIVSSSFYDAGNGIATDVAGNVYITG
                     SFAGTADLDPGIDSAFVTAASSSPDIYILKLDAGGNFLWGGRAGDIGNDAGEEIVTDA
                     SGNIFTAGSFSRTADFDFNTGVNNLTTVNTLSNVYVLKMNQNNVGIYENMIQNKFTVY
                     PNPATNILKINNLKFEIGSAIKIFDVMGKEVLHTVVGGAETAINVSKLESGIYFVHLF
                     SVSAPEENILTEKIIIH"
     gene            complement(8027..9760)
                     /locus_tag="PKU82_07600"
     CDS             complement(8027..9760)
                     /locus_tag="PKU82_07600"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82773.1"
                     /translation="MKKTLLTLCCFSALTLNAQTPTFDWAINIGSTNFDKGYDIATDA
                     AGNVYEIGILAGTADFDPGAGVVNLASNGNDDIFIAKYSTTGNLLWAHNIGGTGNETA
                     VAMALDASGNCYFTGHFTGAVDFDPGAGTSIITASGAFDVCIVKLNSSGNLVWAKNFG
                     GTYGTFVDELSLDASGNVYTEGTFQTTTDFDPSAGVYNLVSTGGQDPYLAKLDAAGNF
                     VWAINTGFSVNSLHIDNNNTVYLAGSFNDTTDFNPNATQVFNVIPIGATDIYIAKFDT
                     AANFIWVKNIGGTTGTDGGNAYAINTDAAGNIIVTGTFTGTVDFDPGTGTANLTSVGT
                     GTNMSDMYFLKLDASANFVWVKRVGGNSIDVPLDIATDNAGSVYFSGRFKSSIVDFDP
                     DPTVTFNLNGTFSSYDAFVLILSATGNFVYAYEIGGPSNGDDEANAICVNPTGSFYVT
                     GTFYGPIDFDPGTGTTTLNDIGNTDAFIQKMNFSTVGLAHLTDENYFILYPNPANQLL
                     VVNHGLLKENCELKIFDVTGKEVHTSTISGNQSTIDVGKLENGIYFLQLSYSSPSMKS
                     SGASQKLIIHH"
     gene            complement(9862..11082)
                     /locus_tag="PKU82_07605"
     CDS             complement(9862..11082)
                     /locus_tag="PKU82_07605"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOZ82774.1"
                     /translation="MRNLNTLKLIFTLVIVGSLSMNSIKAQTWTQQTSNTTEDLNSVH
                     FFDANVGGAAGFNGTILTTTNGGTTWTNQTSGVTVNLFDIFFADSQTAYSVGLGGTIL
                     KSTNGGTTWVSQTSGTTQLLLDVFFVNPLEGWVVGNSGTILHTINGGTTWTPQSGMNN
                     AFKAVYFISPTEGWAVGYPLILHTTNAGTTWSQQTAPVVHYNDVHFINATEGWVVGEN
                     SIGLKTMDGGITWNSSSFSFQTSIIAVHYVSSSVGYINLEASLPSNNTLRKTTDGGVT
                     YQVEPTGVNARTTDSYFINAGLGWVVGYDGVILKYNSGTGIDNINDNSNINIYPNPAT
                     NKFEVKGLMFDGAYELRIFDVMGKEVLKTNVIGEKSIIDVTNIKNGIYYVQISNSSSL
                     GKSAGAYQKLVIQH"
BASE COUNT         3549 a         2090 c         2008 g         3666 t
ORIGIN      
        1 aaaaaaattt gttttctcat tgtattgtaa aagacctgat gaggtaatta tccataataa
       61 ggagtctcct gtagatttga ttccttgcat agatgtgata tcagaatcaa ttattttaaa
      121 aggagaggtt ttattttctt tactaaaaag taccaagcct gccccctgtg tcgaaagcca
      181 ataggttcca tctttacgtt cagcaatatc aaaaacattt tttattttta ttggaattga
      241 gttcggcaac atattccaat ttgttggcat caccgttatt ggtaacatgg aatcattatc
      301 agctgcatat tttgataaac taaaatcgct cttgaattta agttggacaa gaccttttga
      361 tctgccacaa acccagaaat tgttttttga atcaacccaa attttgcgac ttaaattctc
      421 taaattgtcc gatatttttg tattttcatg gataagatag tacaagtttc gattatcaaa
      481 gtcaattaat acaatgtagt tgccatagta agatgcagca attaattctg aattgtcttg
      541 tttgaaactg gtgagagaat tgactaatga tgcaccatta aagtaaaatg aagtttcttt
      601 cagattttta ttaaagtcta ttgtattgaa tgaaatattt tcaggattaa atttgaaaac
      661 agttctgtta ctgccaatgt aaatatcacc attcttcgtc atcagaatac tatataattc
      721 ctgctcttca tttcgataaa taacagtttt gaacttatag gattcatttg gagatttttt
      781 ataaaataaa ccacattggg taatgacaag gtctaaacct ttgtcagtaa tgagtctgtc
      841 gaatactttg caagcattaa ctccttttgg cacaggaagc atttctgttc tgaattgatt
      901 tctatgctga tcatacacta acaaaccgtt ttctgttgca atccagattc tgttttgtga
      961 atcacgaaac aattcgttta cacgggtatt aatctgatga gaaaaattta tattgtttat
     1021 aaatgattta gaagctttgt taaaaataaa tatgcctgat gattttgttc cacaccagat
     1081 gttattgctg gcatcagaag aaattgaaat aatctcttcg ccagattttt taaatttaga
     1141 tgttccgtca aagatgtacg attggaggcg cttaccatgg atattgaata gatgcagtgc
     1201 attatcccat gcgccacacc aaacattcaa atcattatca atataaattg tattaatagt
     1261 tgtagctgta tcaccaacca tacccttatt ccttccaacg ttatctaatt tttttagtga
     1321 ttctattata cctgcaaaaa gtgaaatagt gtctttaaca gcaacagcgt aatatgagtt
     1381 gattgaatat acatagggat tgagtttttt atttttcaaa tcaaattgtt gcagacctcc
     1441 attattcatg gcaacccaaa tacaattacg tactttgtca aacgctaaat cgttaatata
     1501 ttcagcacga aggggactgt tgcgggcatg atagttttga aaagtatttg ttttgtaatt
     1561 ataactgcaa aaaccattag atgccgttcc aatccataat aaagagtcgt ctgtaacttc
     1621 aaggcaggta atattgttgc cactaacaga gtttttatca ctttgaaatt taaatacctg
     1681 aaaacttttt ccgtcaaaac ggttcagccc atcttttgta ccaatccaga tgaatccata
     1741 tttgtcctga atgacacaat ttgtttgatt gtcggataag ccatcattag cagtaaattc
     1801 attgagattg atggttaatg aatccgccaa tgcaggtaaa gtaaaaacca gaaatagaag
     1861 tagtgatttg aaaaatttaa tcattcggtt ttggcaagga attgcctaaa ataaataaaa
     1921 tcttctattt aaagaaaacg attggtgaaa ttaaaagtag aagtggttaa actatggatt
     1981 tgtgtggtgt aatgatgtgt tgttgtttaa tcacacagaa gctttaataa ttttagtcgt
     2041 gtacagaaaa acgctttgta acagaactgt tgctattaga aatttttaaa acatatatgc
     2101 cattagcaag attttgtgtt tggacatgaa aaatattttc tgttccattt aattcttgcg
     2161 ttgaaattat tgctccatta atatcataaa cttctgcggt taaattcttt tcgacattac
     2221 ctgttacttt aaaatagagg ttgtttttat tcgtaaatac agcatagttt atttgtagct
     2281 gttcatagtt gtttagttca acagcaactg aattgctcaa atggctttca ccattaaaat
     2341 caaattgtct taaacgatag aaagattttc gtgctaatgg ttttgaatct gttgcatgat
     2401 atcgtaaaag ctgattagaa aatcctgcag cctgtgtttt attaattggt ataaagttta
     2461 agtcaacagc agcacgttct atttcaaagt aatcactatt tatttcagat actgttgacc
     2521 atcttaattc aacttggtca ttatttagtt gtgctgtaaa attcagcaac tcaacaggta
     2581 agggattttc tgaagatgcc gatgataagg taaatggtcc aaataaattg tctgctgtgc
     2641 ttgtagtaat ggtacctgct gacacatttc ctgttgtgct gccattgcct aaattattcc
     2701 atatagttcc aacactgcgc caacgagcaa ctcttaaatc gctgagtaag gttactccac
     2761 aactggaagc atcccatgat aatgttacat ttcgacttga acttcctgca atttgattaa
     2821 gcgtccagaa ttcgcactga ctgatgtggt caagtgttgg atcaaaaata ttactgtatg
     2881 ttatttgcgg attagcacgc atatattctg caacaaactg acttgaagtt aaagaactta
     2941 gcgaaatatc atctatggca aaggatggat cggtgcctac tccatcatca ttgttttccc
     3001 atttcaaacc aattcgcaca ttagcattat tatttgcact tgccggtagt accattgaaa
     3061 atgctgtcca ttttccttgc ctgaatccat tacatgctcc accgcaacat gcagtctttg
     3121 gcatatcggt aatttgtgcc catgcggcac catcataata ccaaagggta gcattgtcaa
     3181 ttgtaccatc gccaaattca atgtaattaa aactgatgct aatgttactt tttcctgtgc
     3241 aattgattac aggcgattgt gccattctgt ttgtttgggg gcagtataaa agtccacata
     3301 acccacctgc atcatatgca gctccacctg ttggattaaa tacactacta acatgcatgg
     3361 tattgtttcc attatttgca actccacaac cacctgcagc aacaccacct tctgcatcac
     3421 ttattacaaa aggatttgga tcagcacctt cgggccccat aacgttaaca agagaccagc
     3481 ctgctccggt attaaaattt tctgtccaga aagatgacga tgatgcatca gcgccaatag
     3541 caattgaccg atatgaagta ttttttccta caggaaaagt gaatgcctgt gagccgtttt
     3601 tttgcaccgg gccatttaca aaactaacat ttgatgctcc tgttgcagtt gcaccatttt
     3661 caactattaa aatattggga gttgaagaat ttataatacc tgttattaag ttcagattgt
     3721 tatcaacctt tactgatttt aaaagggtaa ggctgttggc agacttattc atttcaagtt
     3781 gataaatctg tagcgatgta agtgctgcag agcctgatat agattgtgca ttaccaccac
     3841 tgaatctata cttacgtgca ttagctgcac cataacttgt ggagccattt acagttaggt
     3901 ttccgaaaat ttcaaaccct gttccgaaat ttctgagatt gcatccacca cgtattagta
     3961 tattgccctg aacttgtacc ggtgtagcaa aattgttatc atttaaaaaa tcaacagtgc
     4021 ctaatagtgg agctaacaca ccaccaacat caaaattacc tttgatagtt gcagtagctg
     4081 tgctgcctgt gaatgttgat gaaacagcag ttgtccaaac acctcccgaa gtattcatga
     4141 tagtgaggtt tggataaaac atatttaaag ttgttaatga aggtgcagta actgaactgg
     4201 gtgttttatt taaaacccaa cttgcagttg cagggatagt gcttatgcca ccctgataat
     4261 tgttttgcca gtttgttgca tttgtattct ctgttttaac ataagtgcct gatgcaccta
     4321 atgcccatgt tgctccagct agccaggcaa cgctggttga acttccatct gtaaatgtac
     4381 cgttaacctg taagtcaaca cctgtaccat tattgattgt aagcagtttg gaattgtaat
     4441 ttacagttcc accattgtta atagttgttt catcaatgcg tacattggcc gaaatggtta
     4501 ctgtatgtcc actgcgaatg gtaatagttt tagcatagta gtttggtaaa aaaacagctg
     4561 ctgtccacgt aactaaatcc ggtgattgtt gccatgttga taatgaattc catgcaccgg
     4621 aagtgatact gcgataatga ttttgtgcat cgttggcttt aataattaca tcatcaattc
     4681 ttgatgaacc accggaagcc acagtgctgt tgttaacact tgtattcgtt gtcattatcc
     4741 aacgtaaaaa aactgaagct tgattttcac atgcaacagg taaggggata tcggtgacaa
     4801 cacctgaagt aaaattgtct gccacaacaa ttgcaccggc accgggaatt ggtgtccatg
     4861 tgccggcaat accaatctta tattctatag caaagttctt gggccctgtg ggcgaactgc
     4921 gttgtttaga ggataattca agattaaaat aacctgttgt attaatttct gtctcccaat
     4981 attttgtgcc ggagccatta tcccatcctg ttgtccaagc actttgagtt gtggcaccgg
     5041 cagttgtata aacaactgcg gcagtacctc ctacggtgta aatggttttt gcactattag
     5101 caggaataga aatatctaca gtagcattat caggattgtt tggaaaattc cattcagcca
     5161 atgttaattg agcagaagcg gctttgacat aaagtagaaa tgccaatgaa ataaataaat
     5221 tggctactct tttcatgttg ttttttattg taaaatcaaa gatatgagaa attattacta
     5281 atccaacatt taatagataa aacttatgaa agtaaaaaac tgataatagg atgaaggcat
     5341 aagggggtga atgttgcaga aaaggatatc cttaaaaacc ggagatttta aaggagatta
     5401 tgcaatgagt atataggaat ggtgccttca agaaaggctt acaaaaaaac aaggctgccc
     5461 aatcgggaag ccttgttgta agagaattat ggggttaatt aatcctttat cagtttataa
     5521 gtctgaacca cattgcttga atgtaattgc aacagataaa ctccttgagt gtagttagat
     5581 aaatcaacag tagtttcatt gcctgaaaac aaagtattaa tcaatgtacg accttgtaag
     5641 tctgttactg ttatctgaac attttcaagt tgattgtcgg attgtacatt aaatactcct
     5701 gaagaaggat ttgggaagat gttgagtaat gtgctgttgc tttcaatttt aaaagctgga
     5761 tgaacaaaat caaatgcaaa ttttcttttg ccttttttca atttcaactt caaacccgaa
     5821 ttttgatggc aatcctgttt ttaaaatctc attggagggt tgttaaggat taactaacta
     5881 aataaacagt attgttattg tcaatatgta acgagtttac agagaagcaa gtgtttattg
     5941 cccagacaaa atttcctgct gcatcaaatt tgattaagaa aattgcattg acgttgttac
     6001 cgcctggtgc tgtcagattt aaaacagctg ggcccggatt aaagtcaaca gttcagaaaa
     6061 gaactggtta cataaaggtc accgttaaag ggatcttttg taattgattt tcctttttca
     6121 ttagaggcca ctcccatagt ctttacccat gataaagatg gcatttgtga atttgatatt
     6181 gtaaatgata caacaaacaa acatgccatt aatcaggata ctgatttgtt gtaaaattta
     6241 ttcatgactt ttcagtttat attttagtga atgataattt tttctgttaa gatattttcc
     6301 tccggagcac taacagaaaa taaatgaacg aaataaattc cactttctaa tttgcttaca
     6361 ttaattgctg tttctgcacc tccaacaact gtatgtaaaa cttcttttcc catcacatca
     6421 aaaattttta ttgcactgcc tatctcaaac tttaaattgt ttatctttaa aatgtttgtt
     6481 gcagggttgg ggtaaacggt aaacttgttt tggatcatgt tttcatatat gcctacatta
     6541 ttttgattca tcttaagcac ataaacattg gaaagggtat tgacagtagt aagattatta
     6601 acaccggtat taaaatcaaa atcggcagtg cggctaaaac tgccagcggt aaaaatgttg
     6661 ccggatgcat cggtaacaat ttcttctccg gcatcgttac ctatgtcacc ggcacggcct
     6721 ccccataaaa aattgccgcc tgcatctaat tttaaaatat aaatatcggg gctgctggag
     6781 gcagcagtta caaaagcaga atcaataccg gggtccaaat cagcagtgcc tgcaaacgaa
     6841 cctgttatat atacattgcc ggcaacatct gttgcaatgc cattgcctgc atcataaaac
     6901 gaactactta ctatttgttt tgcccataag aaattaccgg cagcatctga ttttaaaaca
     6961 aaaacatctt caaaaatact ggtggtaaga ttaaaagtgc cattgcccca ttcaaaatct
     7021 actgcgtctt caaaataacc tgttgtaaat acattgccat tggcatcaac acttattgcc
     7081 tggcccacat cttcggatgt tgctccaaaa ttttgcgccc aaataaaatt gcctgttgaa
     7141 tctaacttta aaataaaaat atcggggtcg cttgccacaa actgattgct gcgcggagca
     7201 attaaattaa acacacctgc acccgggtca aagtcaaccg tatcggaaaa agagcctgtt
     7261 gcgtgtacat tacccaaggc atcaatagct atggaatttc caatgcagta attgaaccct
     7321 ccaaaatttt ttgcccacac aaaatcacct gtggcattta gttttgaaat aaaaatgtcg
     7381 caatatgggc cagcattaag cgtaacaacc ccgggtcctg ggtcaaaatc ggcactaagt
     7441 tcaaagctac ctatggtgta aacatttccc caattgtcgg ttgtaatatc ataacccaca
     7501 tcccggctgt taatagatga tgccgtactg cctatatttt ttgcccatac aaaatttccg
     7561 tttgtgttaa gatgccatat aaatatatca cgcccaccgt ttgaggtcag gttaaatgtg
     7621 gcagagcccg gatcaaaatc aaccgagtcc ttaaaatgcc ctgtaacaaa tacatttcct
     7681 gcatcactgc atgtaagcgc aacctcactt aaatttccaa tccatccggt ggttttaaca
     7741 gcccattcaa aattgcctgc tgtatccaac ttcaacacaa aaagctctaa gccaactgaa
     7801 cttgttaaat tatacacacc agggccggga tcaaagtcaa ccgttttacc gaagcaaccg
     7861 gctacatata aatttcctgc tgcatcgcgt gcaatagatc ttccttggtc acctgccgaa
     7921 tcgccaactt gttgtaccca ttcaaatgcc ggtgtttgtg cctgcacaag cagggttgtt
     7981 gccattacga aaatggtaag cagtgttttt ttcatttgac tttagtttaa tgatgaatga
     8041 ttaatttttg cgatgcacct gatgacttca ttgaaggaga ggagtatgaa agctgtagaa
     8101 aataaattcc gttctccaat ttgcctacat caattgttga ctgattaccg ctgatggtgg
     8161 atgtatgaac ttcttttcct gttacatcaa aaattttcag ttcgcagttt tcttttaata
     8221 atccatgatt aactaccaat aactgattag caggattggg ataaagaatg aagtaatttt
     8281 catcagttaa atgagctaaa ccaactgtgc taaagttcat tttctgaata aaggcatcag
     8341 tatttccgat atcatttaaa gtggtagtgc ctgtgccagg gtcaaaatct atagggccgt
     8401 aaaaagttcc ggttacgtaa aagctacctg taggatttac acaaatagca ttggcttcat
     8461 catcaccatt agatggacct ccaatttcat aagcataaac aaaatttcct gttgctgaga
     8521 gtatgagtac aaatgcatca tagctgctaa atgtaccgtt gaggttaaat gtaactgttg
     8581 gatcagggtc aaaatcaaca attgaagatt taaagcggcc cgaaaaatat acagaacctg
     8641 cattatcagt agcaatatcc aaaggtacat caatagagtt gccacccact cttttaaccc
     8701 atacaaaatt ggctgaagca tctaatttta gaaaatacat atcagacata tttgttcctg
     8761 tccctacaga tgtaagatta gcagtgcctg taccagggtc aaaatcaact gttcctgtga
     8821 atgttccggt aacgataatg tttccggctg catcagtatt gatagcatat gcatttccgc
     8881 catctgtacc tgttgttcca ccaatatttt taacccaaat gaaatttgca gcagtatcaa
     8941 atttcgcaat ataaatatct gtagcaccaa taggaataac attaaaaact tgagtggcgt
     9001 taggattaaa atcagtggtg tcattaaatg atccagctaa ataaacagta ttgttattgt
     9061 caatatgtaa cgagtttaca gagaagcctg tgtttattgc ccagacaaaa tttcctgctg
     9121 catctaattt agccagataa ggatcctggc caccagtaga tacgaggtta tacacacctg
     9181 cagaaggatc aaagtcagtg gtggtttgga aggtgccttc ggtgtaaaca ttgcctgaag
     9241 catctaaaga aagttcatca acgaatgttc catatgttcc accaaagttt ttagcccaaa
     9301 ctaaatttcc tgatgagttt aatttaacta tacaaacatc aaaagcgcct gatgctgtta
     9361 tgattgatgt tcccgctccc ggatcaaagt caacagctcc tgtaaagtgt ccggtaaagt
     9421 agcaattgcc cgatgcatca agtgccattg ccacagcagt ttcattacca gtgcctccta
     9481 tattatgcgc ccataacaga tttccggtag tgctgtattt agcaatgaat atgtcatcat
     9541 ttccattaga agcaagatta acaacgcctg ctccggggtc aaaatccgca gttcctgcta
     9601 atattcctat ttcataaaca ttgccggcag catcggttgc aatatcatag cctttatcga
     9661 aattagtact gcctatgtta atagcccagt cgaatgttgg cgtttgtgca tttagcgtta
     9721 gtgctgaaaa gcaacaaagt gtaagtagtg tttttttcat ctgtatataa tttttgaatt
     9781 ttaaattgca agactttttt ttataagaat caattgctga ttgtgatttt taaatataga
     9841 gaatttaatt gttacactga tttagtgctg tatgactaat ttttgataag ccccagcact
     9901 tttacctaaa gaggaagagt ttgaaatttg aacataataa attccatttt taatattggt
     9961 tacgtcaatt atagatttct ctccgattac gttcgtcttt aaaacttctt tacccatcac
    10021 atcaaaaatt ctgagttcat aagcgccatc aaacatcaga cctttaacct caaatttatt
    10081 tgtggcagga tttgggtaaa tatttatgtt agaattatca ttaatattat caatacctgt
    10141 tccggaatta tattttaaaa ttacaccatc gtagcctacc acccatccta agccggcatt
    10201 tataaaatat gagtcagtgg ttcttgcatt tacacccgtg ggttctacct gataggttac
    10261 ccctccatct gtggtttttc tgagagtatt gtttgagggt agcgaagctt caagattaat
    10321 gtatcctacg cttgatgata cataatgtac agcaataatg ctggtttgga aactaaaaga
    10381 agaagagttc catgttatgc caccatccat tgttttaagt ccgattgagt tttctcccac
    10441 aacccaacct tcggttgcat ttataaaatg gacatcgtta taatggacaa caggtgcagt
    10501 ttgctgagac caagtggtac ctgcattagt tgtatgtaat attaaaggat atcctacagc
    10561 ccatccttca gttggtgaaa taaaatatac agccttgaaa gcattattca ttcctgattg
    10621 tggtgtccag gtagtgccgc cattaatggt atgtagtatg gtgcctgaat tacctactac
    10681 ccatccctct aaagggttaa caaaaaaaac atcaagcaaa agttgtgtag tgcccgatgt
    10741 ttgagaaacc catgtagtgc caccattggt tgatttaagt atagttccgc ctaagccaac
    10801 agaatatgca gtttgggagt cagcaaaaaa aatatcaaat aggtttactg ttactcctga
    10861 tgtttgatta gtccatgtgg ttcctccatt agtggtggtt aaaatagttc cgttaaatcc
    10921 ggcagcaccc cccacattgg catcaaaaaa gtgaacgcta ttcaggtctt cggtagtgtt
    10981 gcttgtctgc tgtgtccatg tttgtgcttt tatactattc attgataagc taccaacaat
    11041 taccaatgtg aaaattaatt ttaatgtgtt taaatttctc atatttttca gtttaaattt
    11101 taaaaacaat tatgacccta aatatcggaa ttatttttta caaataaata tttatttatt
    11161 agtttctgca atcaccaatt ctggtgattt tgaatattaa caaaaagaat attgaaatca
    11221 gattaaaaag ttcttataaa aactttttaa atcaataaaa aatcagaaac aattaaacag
    11281 cagtaagcgt tagctaacta aaatgtagaa aac
//
LOCUS       DAOOOL010000079        11312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_529_length_11312_cov_54.595896, whole genome shotgun sequence.
ACCESSION   DAOOOL010000079 DAOOOL010000000
VERSION     DAOOOL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_11312_cov_54.595896"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1111
                     /locus_tag="PKU82_07610"
     CDS             383..1111
                     /locus_tag="PKU82_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82775.1"
                     /translation="MVQEINIKVLLVKHNQDLFVPITKCLESESIQYTCVCSATEALN
                     ELKNETYDFILLENQLTEYSALIFMKQLNLQRYKGNVMIVAKNATDEEIVSAISHGSD
                     DVFTAPFNTNLILARLKSLYRLRLGNIPQNYFFGEIEINPTEYKVWVNKNALTLTKTE
                     FEILKFLIFNPDRIVPHEVIAKQALNLTPSEIGENYEFLYSHLKNLKRKIGQHSAFNY
                     IKTVYAQGYRFCSFIPSTEGNAEQ"
     gene            complement(1133..1579)
                     /locus_tag="PKU82_07615"
     CDS             complement(1133..1579)
                     /locus_tag="PKU82_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOZ82776.1"
                     /translation="MIELNLPHFDFKIKTEGQSKQIFDQLRNKYVALTPEEWVRQHFI
                     MFLINEKKFPKGLFAIEKEHVFNDLKMRADLIIYKHDLSPWMLCEFKSPDVAITQEVF
                     YQVARYNLTFDVPYLVVSNGLEHFCCKRVEDRFEFQDDIPDFETVD"
     gene            1652..2422
                     /locus_tag="PKU82_07620"
     CDS             1652..2422
                     /locus_tag="PKU82_07620"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOZ82777.1"
                     /translation="MNSKEEIVKNWLPRYTGTAIEDFGKYILLTNFNNYVEIFAEQHQ
                     VEIMGTHRSMPNATANGITIINFGMGSANAATIMDLLTAIDPAACLFLGKCGGLKRKN
                     ELGDLILPIAAIRGEGTSNDYMPAEVPALPAFSLQKAVSTTIRDYDKDYWTGTVYTTN
                     RRVWEHDEEFKQYLRKMRCMAIDMETATIFTTGFVNKIPSGALLLVSDQPMIPDGVKT
                     EKSDLNITRKFVKDHINIGIDSLNQLKNNGLTVKHLRF"
     gene            2429..3493
                     /gene="holA"
                     /locus_tag="PKU82_07625"
     CDS             2429..3493
                     /gene="holA"
                     /locus_tag="PKU82_07625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOZ82778.1"
                     /translation="MPERKFEEILKDLKNKVYHPLYFLQGEEPYYIDAISDYVEKNVL
                     NDMEKEFNQTILYGREADAMAIVSAARRFPMMSNYQVVIIKEAQDVKWFGSKEKEEDK
                     TKDKSKEKEDPMMLYLQNPTTTTILLFCYKYKTIDSRTKKGKLIKDKAQFLETKKLYD
                     NQLPAWIKNYLKDRKIKADDHAIEMLAEYLGNDLSKINNEVGKMLINLKDKSVITVND
                     IREHIGVSKDYNVFELQNALAERNILKANRIVNYFEANPKNNPIQLIIGSLFAYFNKI
                     LIYHFTADKSSASLASAIGISPFFVKDYERAAKNYPVRKLEQIIGWLREYDLKSKGVG
                     STDQVPTGELLREMVFKIIH"
     gene            3497..4234
                     /locus_tag="PKU82_07630"
     CDS             3497..4234
                     /locus_tag="PKU82_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOZ82779.1"
                     /translation="MIRVSVVSYLNSKPFVAGLTQNSIKNKIDLSLDIPSVCAEKLIN
                     GKVDIGLIPVAVLPLVKNAQIITSFCIASNGNVNSVLLLSQVPLNEIKQIWLDYHSRT
                     SVMLAQLLAKEFWKIDVSWLPSEKGFENKIEGQTAGVVIGDRALALKSNFKYVYDLAT
                     EWKKHTGLPFVFACWVSNKSLPEDFIVAFSHALNQGVAMIPELSKQLQPDYLFDVKSY
                     LTENIDFRLDEEKEQALKLFLERIELK"
     gene            complement(4287..4808)
                     /locus_tag="PKU82_07635"
     CDS             complement(4287..4808)
                     /locus_tag="PKU82_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ82780.1"
                     /translation="MKNSDIIFKDSVKDHYRSLRGFALKLTQNTEDANDLIQETMLKA
                     LNNEDKFEKGTNLSGWLYTIMRNIFINNYRRMVKSQTFLDSTENQFFLNNIKNATRNR
                     GEANLVMKEINSAINDLNDNLKVPFMMSYRGYKYEEIASQLSIPLGTVKIRIHTARQK
                     LKEKLNVYNSLSF"
     gene            complement(4936..5709)
                     /locus_tag="PKU82_07640"
     CDS             complement(4936..5709)
                     /locus_tag="PKU82_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ82781.1"
                     /translation="MKISIVTITYNSAATIEDTIKSVVVQDYPDMEYLLIDGKSKDST
                     LQIAEKYKDKITKIVSEKDGGLYYGLNKGLKMAAGDVIGAIHSDDLYAHPQVVSNVMK
                     LFNADPELEGVYADLIFVNREDTNKVMRVWKAGEYKPDSFEKGWMPPHPTFFVRKSVY
                     EKYGYINTDLKLSADYELMLRLIHKHKIKIAYLPETIVKMRMGGVSNVSFFVKLKANI
                     EDKLAWKMNNLKPGLFTTLRKPLRKISQYFKKAYNTFFY"
     gene            complement(5764..6918)
                     /locus_tag="PKU82_07645"
     CDS             complement(5764..6918)
                     /locus_tag="PKU82_07645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82782.1"
                     /translation="MPARKKILIAIDWYLPGYKAGGPIRSCASIISCLKNDFDFAVIT
                     SDTDFSDDKPYEGIKSDTWNILPDGTRVFYFSKNNFSQKSLREVIQKEKPDILYMNSF
                     FSQDFTLKPLKIARKYLPECKCIIAPRGMLGAGALAIKPLKKLAFISLSKMLATFDKV
                     RWHASTEQEAKEIKAIFGNRAEVRVALNLSSVKPAVSETKIKNAGQMNLVFFSRISKK
                     KNLHLVLQWLEKIPEHLKITFHIYGTLEDDAYWSQCNDLIKLLPPNITVEYKGVVDAG
                     KSESVFENNHFSILPTMHENYGHAIVESLAFGVPVIISNNTPWHGLEAKHAGFDFPLE
                     NQQQFIDSIVKAASMDQKTYKLWQQGAMELAKEVLNNSDTVNQNKKLFAE"
     gene            complement(6918..8783)
                     /gene="asnB"
                     /locus_tag="PKU82_07650"
     CDS             complement(6918..8783)
                     /gene="asnB"
                     /locus_tag="PKU82_07650"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ82783.1"
                     /translation="MCGITGIYGMSSEKSASTIRLMNNAISHRGPDDEGYYVDEQVAL
                     GHRRLAIIDLSPAGHQPMHSNDGNLEIAFNGEIYNFQEVKKLLSGYAFKSGSDTEVIL
                     AAYQQWGKDCLQHFNGMFAFAIWDKAKQELFIARDRLGIKPVYYFRQDKTLLFSSEIR
                     SLLQSGIVPRKINSKVIYEYFTYQTIHAPETFINNVFMLMPGHYMTVNASGINIERYW
                     HIVENTDFSSSGKSYQEVCADINKLFFDAVNRRLISDVPFGAFLSGGIDSSAVVGAMS
                     RIHSQPVKTFTIAFDESDFSEAKFAKLIADKFKTDHHEFLLKPEDFLNQLPEAMNALD
                     HPSGDGPNSYIVSKITRQAGVTMALSGLGGDELFAGYPVFKRTELLQQKSWLWKLPLP
                     LRQLLGKLITTLKQDTASIKQQQLLLSPDASLQYSFPVSRQVSNAKQLASFIEEKFNG
                     IDAVQEIVRELNKHKALPLFSQVSAAEISSYMQNVLLRDTDQMSMAVALEVRVPFLDY
                     KLVEYVMGVNDEYKNPVYPKKLLVDSLGDLLPDEVVHRKKMGFVFPWERWLKKELHTF
                     CGDRIYALANRGFMNKEALNNRWQSFNEGKPGVRWLDMWLCVVLEHWLEKNNIEY"
     gene            complement(8787..9857)
                     /locus_tag="PKU82_07655"
     CDS             complement(8787..9857)
                     /locus_tag="PKU82_07655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ82784.1"
                     /translation="MPIAKKKALFLYLEPAGYVEACFKKTALNYPLDLHIVKYPLDPN
                     APFRFDDVESITYYDRNQYQEQKLNDLVASINPDFIFCNGWMDKGYVNVAKQYSGKIP
                     TVLAFDNLWEGKLKQYVASVTAPFILPRIFSHCWVPGQPQVIYAKKLGFKENRIETGM
                     YSANYELFDQAYQKIMPIKEKNFPKRFIFVGRYIEIKGIRELWQAFTQLQNEMPNEWE
                     LWCLGKGPLEKEFPQHPKIRNVGFVQPSAMEEYMRQTGVFLLPTYDEHWGVVVHEYAI
                     AGYPLLLSTKAPAGTAFLKQGENGYYHEPSNVDSLKEALKKIILLPDSTLIEMGKKSH
                     ELSKSMTPDTWAAVVWKILNSK"
     gene            complement(9817..11136)
                     /locus_tag="PKU82_07660"
     CDS             complement(9817..11136)
                     /locus_tag="PKU82_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ82785.1"
                     /translation="MLQFIRNNAQFVIMCFTWVFCGMVNETLGVAAVVLSVIMLKIKG
                     MYKELILGLIFLLIMSDSRQFEALYAAQAKNFYIVLLSVFYFFDRKNFTGTNDYFKPF
                     IPFFLWSIAMLMQAPTVNLMNSAMKTLSYILLFTVVPAYFLKAFRELGESFLRDVIML
                     FCCILLAGIIMIPLFPNTVFLVGRFSGVYGNPNGIGIGCTLLFILVYISLKKFPGLLD
                     QNEVRLIYGLIILSTLLAVSRNTMMSIILFLLFARFYEMGYLAGFAILVVALVAYELI
                     NQNIVAIVEALGLGHYLRAEAIEDGSGRLIAWRAAWKQIQSQFFLGRGFAYDEWYFYA
                     NRHWLSALGHQGGVHNSWLALWMNTGLIGLILFAVGFIKTFVRAMHHSKLAFPILFAV
                     IFSASFEAWIMGSLNPFHIILILVLNLIIHYNQFNADREEESLVSVS"
BASE COUNT         3734 a         2107 c         1895 g         3576 t
ORIGIN      
        1 atgcaagtaa tcggatgaaa tcaactgtga aatacttctt ttattcattt aaaaaaatca
       61 tttcatacgc taatacaagc atctgtcttc aaaacacaat taacaattgg acacaatcga
      121 ttaattaagt cgtttatggc ttgatttttg ttgtagtttt agtgaagtta ctaataaatt
      181 ttaccaccca cctttttcta aaagtcataa catttttttg gaaatgttaa taagttattt
      241 ggcgaacgtc cattttttat gggtgagtat ttagttttgt tgcataagtg agtaaatcgc
      301 tggcatgctt gcccatctta aatgtcgttc gcagcaattg atttaaacta attggattca
      361 acaacaaaca taacaaccat ttatggtaca ggaaatcaac attaaagttt tattggtaaa
      421 acacaatcag gacttatttg tgccaattac caaatgcctt gaaagcgaaa gcattcaata
      481 cacatgcgtc tgttcggcaa ctgaagcact taatgagcta aaaaacgaaa cttatgattt
      541 tattctgctg gaaaaccaac ttacagaata cagtgcgcta atctttatga aacaactcaa
      601 cctgcaacga tacaagggta atgtgatgat tgttgctaag aatgcaactg acgaagaaat
      661 tgttagtgcc atcagtcacg ggtctgatga tgtttttaca gcaccattta atacaaacct
      721 gatacttgcc cggttaaaaa gtctgtatcg cctgcgattg ggcaacattc ctcaaaacta
      781 tttttttgga gagatagaaa ttaatccgac agaatataaa gtgtgggtaa acaaaaatgc
      841 attgacattg actaaaacag aatttgaaat tcttaaattt ttaattttta atccagatag
      901 aattgtgccg catgaggtaa ttgcaaaaca ggcattaaac ttaactcctt cagagattgg
      961 cgaaaactat gagtttctat acagtcatct taaaaacctg aaaagaaaaa ttggacagca
     1021 ctctgctttt aattatatta aaactgttta tgcgcaagga tatcgttttt gttcatttat
     1081 tccctcaaca gagggtaatg cagagcaata aaatattaga aatccagcta ctttaatcta
     1141 ctgtttcaaa atcaggaata tcgtcctgaa attcaaatct atcctctacc cttttgcagc
     1201 aaaaatgctc taaaccatta cttactacaa ggtaaggcac atcaaaagtc agattatatc
     1261 gtgcaacctg ataaaaaact tcttgtgtta tagcaacatc aggagattta aactcgcaaa
     1321 gcatccaagg tgataaatca tgtttataaa taatcaggtc agcacgcatt ttcaagtcat
     1381 taaaaacatg ctctttctca atggcaaata atcctttcgg aaattttttt tcgttaatta
     1441 agaacatgat aaaatgctga cgcacccatt cttcaggtgt cagggctaca tacttatttc
     1501 taagctggtc aaaaatttgt ttactttgtc cctctgtttt aattttaaaa tcaaaatgag
     1561 gcaaattcaa ttcaatcatt gaacgaaatt aaaaaaactc attgaaatta ctgtctaaac
     1621 agatgattaa gctatcaaga aataaaaatt tatgaattcg aaagaagaaa ttgtaaaaaa
     1681 ctggctgccg cgatatacag gtactgctat tgaagatttt ggtaaataca tattactcac
     1741 caacttcaat aattatgttg aaatttttgc ggaacaacat caggtagaaa tcatgggcac
     1801 acatcgttcc atgcctaatg ctaccgctaa tggtattaca ataattaatt tcggcatggg
     1861 cagtgcaaac gctgcaacaa ttatggattt gcttacagct attgacccgg ctgcatgttt
     1921 gtttcttgga aaatgtggcg gactaaaacg taaaaatgaa ttgggcgatt taatacttcc
     1981 tattgctgca attcgtggag aaggaacatc taatgactat atgcccgcag aagttcctgc
     2041 attgcctgca tttagtttgc agaaggccgt gtcaacaacc ataagagatt atgacaagga
     2101 ttactggaca gggactgttt ataccaccaa ccgaagagtt tgggaacatg atgaggagtt
     2161 taaacaatac ctgcgtaaaa tgcgttgtat ggctattgat atggaaacag ctacaatttt
     2221 tacaacagga tttgtcaata aaattccttc gggagcatta ttgttagtaa gcgatcagcc
     2281 aatgataccc gatggtgtta aaacagaaaa aagtgatttg aatattacaa gaaaatttgt
     2341 aaaagaccat atcaatattg gtattgattc actaaatcaa ttaaaaaata acggtcttac
     2401 tgtaaagcat ctgcgttttt aatagactat gcctgaacgc aaatttgagg agatattaaa
     2461 agatcttaaa aacaaggttt atcatccgtt gtattttctg cagggcgaag agccttatta
     2521 cattgatgcc atcagcgatt atgttgaaaa aaatgtgctg aatgacatgg agaaagaatt
     2581 taatcaaacc attctttacg gtcgcgaagc tgatgctatg gctattgtga gtgctgcaag
     2641 aagatttcca atgatgtcga actatcaggt tgtaattatt aaagaggcac aggatgtaaa
     2701 atggtttggc agcaaagaga aagaagagga taaaacaaaa gataaaagca aggagaaaga
     2761 agaccccatg atgctttatc ttcagaatcc tactaccaca accattctgt tgttttgcta
     2821 taaatacaag accattgaca gcagaacaaa aaaaggaaaa ctgataaagg ataaagcaca
     2881 gtttcttgaa acaaaaaaat tatatgacaa tcagttgcct gcatggatta aaaattatct
     2941 gaaggacaga aaaattaaag ctgatgatca tgctatagaa atgcttgcag aatatttagg
     3001 gaatgatttg tcaaaaatca acaatgaagt tggaaaaatg ctgattaatc taaaagacaa
     3061 atcggttatt acggtaaacg atattcgtga gcatattggt gtcagcaaag actataatgt
     3121 ttttgaattg cagaatgcat tggcagagcg caatatttta aaagcaaaca gaattgtaaa
     3181 ttattttgaa gccaatccaa aaaacaatcc cattcaactg attattggta gcttgtttgc
     3241 ctactttaat aaaattctga tttatcattt cactgccgac aaatcttctg catcacttgc
     3301 ttctgcaata ggcattagtc cgttttttgt taaagattat gaacgtgccg ctaaaaatta
     3361 tcctgtcaga aaattagagc agattattgg ctggttgcgc gagtatgatt taaaatcgaa
     3421 aggtgtaggc agtacagatc aggtgccaac aggagaactg ctcagggaaa tggtatttaa
     3481 aattattcat taaaaagtga ttcgagtttc tgtagtttct tatctcaact ccaagccatt
     3541 tgtcgcaggg ttgacacaaa atagtattaa aaacaaaatt gatttgtcat tagacatccc
     3601 atctgtttgt gcagaaaaac taatcaatgg caaagtagat atcggattga tacctgtggc
     3661 ggttctgcca ttagtaaaaa atgcacaaat cattacttct ttttgtattg cctcaaatgg
     3721 caatgtcaac tctgtgttgt tgctaagtca ggttccttta aatgaaataa aacaaatctg
     3781 gcttgattat catagcagaa cttcggtaat gctggcacag ttacttgcaa aagagttttg
     3841 gaaaattgat gtcagttggc ttccttccga aaaaggattt gaaaataaaa tagaaggtca
     3901 aactgccgga gttgttatag gcgacagagc attggcattg aagtcgaatt tcaagtatgt
     3961 gtatgacctt gccacagaat ggaaaaaaca tacaggctta ccttttgtct ttgcttgttg
     4021 ggtaagtaat aaatcattac ctgaagattt tatagttgca ttttctcatg cactcaatca
     4081 aggagtagca atgattcctg aattaagtaa gcaattacaa cctgattatc tttttgatgt
     4141 aaaaagttac ttaactgaaa acattgactt cagactagat gaagaaaaag aacaagcatt
     4201 gaaattgttc ttggaacgga ttgagttaaa gtaaatcaaa acgcaatttt aaaatgcctt
     4261 tacgttgtaa tctgaaccct actacctcaa aacgacaatg aattgtaaac gttaagtttc
     4321 tctttcagtt tttgtcttgc agtgtgtatt ctgattttta ctgtgcctaa tggaatggat
     4381 aattgtgatg ctatctcttc gtatttgtat ccacggtagc tcatcatgaa tggaacttta
     4441 aggttgtcat tcaaatcgtt tattgcactg ttgatttcct tcatcaccaa gttagcctct
     4501 cccctgtttc tggtcgcatt cttaatgtta ttgagaaaaa actgattctc tgtgctatct
     4561 aaaaatgtct gactttttac cattctgcga tagttgttaa tgaaaatatt tcgcattatg
     4621 gtgtaaagcc atccgctaag gttggtgcct ttttcaaatt tatcttcatt gtttaatgcc
     4681 tttaacattg tttcctgtat caggtcgttg gcatcttcag tattttgagt gagttttaat
     4741 gcaaaacctc ttaaagaacg atagtggtct tttactgagt ctttaaatat gatatctgag
     4801 tttttcattt gtttaacctt tttaaattaa agctcaacaa acttacgata ccaacatgaa
     4861 ttatgtatta atgtttaact taaataatgt taaagttaaa caatattgca agatgcagac
     4921 aatcaaacaa atggcttagt agaagaaagt attgtatgcc tttttaaaat actgactgat
     4981 tttccgaaga ggcttacgta atgttgtgaa taatccgggc ttcaggttat tcattttcca
     5041 ggcaagttta tcttcaatat tagcttttaa cttcacaaaa aagctgacat tactaacgcc
     5101 ccccattctc attttaacaa tagtttcggg gagataagca atctttattt tatgtttatg
     5161 aattagcctg agcattaatt cataatcggc agaaagtttt aaatcggtat tgatgtagcc
     5221 atacttttca taaacagatt tgcgcacaaa aaatgtaggg tgaggcggca tccatccttt
     5281 ttcaaatgaa tcgggtttat actctcctgc cttccaaaca cgcattactt tattggtatc
     5341 ttcacggttt acaaaaatca aatcggcata aacaccttcc agctcggggt cggcattaaa
     5401 cagtttcatg acattggaaa caacttgcgg atgtgcatat aaatcgtcag agtgaattgc
     5461 tccaattaca tcacctgctg ccatttttaa acctttattg agtccatagt aaagtcctcc
     5521 gtctttttca gaaactattt tggttatttt atccttgtac ttttctgcaa tctgaagtgt
     5581 gctgtccttt gatttaccat caatcagcaa atactccata tcaggatagt cctgcacgac
     5641 aacagatttt atggtgtctt caatagttgc tgcactatta taggttatcg ttacaatcga
     5701 aatcttcata ttcattatgt tccttagtgt cttttaccca ttctacaaaa ataggttgca
     5761 aaatcattct gcaaacaatt ttttattctg atttaccgta tctgagttgt ttaatacttc
     5821 tttagcaagt tccattgctc cttgttgcca caacttataa gttttctgat ccattgaagc
     5881 tgcttttact atactatcaa taaactgctg ttgattttcc aatggaaagt caaagcctgc
     5941 atgctttgcc tcaagcccat gccaaggtgt attgttactg ataattacag gaacaccaaa
     6001 agccaaactc tcaacaattg catgaccata attttcgtgc atggttggca gaattgaaaa
     6061 atgattattt tcaaaaacag attcagattt tcctgcatca acaacgcctt tgtattcaac
     6121 agttatattt gggggtaaca acttaatcaa atcgttacac tgactccagt aagcatcatc
     6181 ttcaagtgtg ccataaatgt gaaatgtaat tttcagatgc tccggaattt tttcaagcca
     6241 ttgcaataca agatgcaggt ttttcttttt agaaatacgg ctgaaaaata ccagattcat
     6301 ctgaccggca tttttaattt tagtttctga cactgcaggt ttaacagatg ataaatttaa
     6361 tgccaccctt acttctgccc tattgccaaa aattgcttta atttcttttg cttcttgttc
     6421 tgtactggca tgccaacgta ccttgtcaaa ggtggcaagc attttagaga gagaaataaa
     6481 tgcaagtttt tttaatggct ttatagctaa tgcaccagca cctaacatac ctcttggtgc
     6541 aattatacac ttacactcag gaagatattt tcgtgcaatc ttcaatggct ttaaagtgaa
     6601 gtcttgtgaa aaaaagctgt tcatgtataa aatgtcgggc ttctcttttt gtattacttc
     6661 acgcaatgat ttctgactga aattattttt agaaaaataa aatacacgtg taccatcagg
     6721 caaaatattc catgtatctg acttaatgcc ttcatatggt ttgtcatcac taaaatcagt
     6781 atcagatgta attactgcaa aatcaaagtc attcttcaag cagctgataa tacttgcaca
     6841 agaacgtatt ggtccgcctg ctttgtatcc cggcaaatac cagtcaattg caatgagaat
     6901 tttttttctt gcaggcatca gtattcaata ttattttttt cgagccaatg ttcaagcact
     6961 acacaaagcc acatatcgag ccaacgcaca ccgggttttc cctcattaaa actttgccat
     7021 ctgttattaa gagcttcttt attcataaaa ccacgattgg caagtgcgta aatacggtca
     7081 ccgcagaaag tatgcaactc ttttttcagc catcgctccc atggaaaaac aaaacccatt
     7141 tttttacgat gcaccacttc atcaggcaat aaatctccta aactatccac caataatttt
     7201 ttaggatata cagggttttt atattcatca ttcacgccca taacgtattc caccaactta
     7261 tagtcaagaa aaggcactct cacttcgagt gctacagcca tactcatctg atcggtatcg
     7321 cgcagcaata cattctgcat atagcttgaa atctcggcag cacttacctg actgaacaac
     7381 ggaagtgctt tatgtttatt aagttctctg actatctcct gtacagcatc aattccatta
     7441 aacttttctt caataaatga agctaactgt tttgcattac ttacttgtct tgaaacagga
     7501 aaagaatatt gcaaagaagc atcaggtgac aacagtaact gttgctgttt aatgcttgct
     7561 gtatcttgtt ttaacgttgt aattaattta cccaacaact gacgaagagg taatggcaac
     7621 ttccataacc aacttttttg ctgcagcaat tctgtacgct taaaaacagg atagcctgca
     7681 aataactcat caccaccaag tcctgatagg gccatagtga caccggcctg acgtgttatt
     7741 tttgaaacaa tatatgagtt agggccatca ccacttggat gatcgagagc attcattgct
     7801 tcaggtaact gatttaagaa atcttcgggc ttgagtaaaa actcatgatg atctgttttg
     7861 aatttatcag caatcaattt agcaaatttt gcttcactaa aatctgattc atcaaatgca
     7921 atggtaaatg ttttaaccgg ttgtgagtgt atgcgactca tggcacctac aacagcactt
     7981 gagtctattc cgccagaaag aaatgcacca aaaggaacat cactgataag gcgtctgttt
     8041 acagcatcaa aaaataattt attgatatct gcacaaacct cctgataact ttttcctgag
     8101 gacgaaaaat ctgtattctc tacaatatgc caataacgtt caatattaat tcccgaggca
     8161 ttaactgtca tgtaatgtcc gggcatgagc ataaacacat tattgataaa tgtttcagga
     8221 gcatgaatgg tctgataagt aaaatactca taaataacct tactgttaat ctttcgaggt
     8281 acaattcctg attgcaacaa tgaccttatt tctgaactga acaaaagtgt cttatcctgt
     8341 ctgaaataat aaacaggttt tattcccaat ctgtcgcgtg caataaaaag ttcttgtttg
     8401 gctttatccc aaatggcaaa agcaaacatg ccattaaaat gctgcaaaca atcttttccc
     8461 cattgctgat aggctgcaag aattacttca gtatcactgc ctgatttaaa cgcatatcct
     8521 gaaagaagtt tttttacttc ctgaaaatta taaatctcac cattgaaagc aatttcaaga
     8581 ttaccatcat tgctatgcat tggctgatgg cctgcaggag aaagatcaat tattgctaat
     8641 cttcgatgac caagagcaac ttgctcgtca acatagtatc cttcatcatc agggccgcgg
     8701 tgtgaaatag cattattcat caaccttatt gttgatgccg atttctcact gctcattcca
     8761 taaatgcctg ttattccaca catagtttat ttactgttta aaattttcca gactactgca
     8821 gcccacgtat cgggtgtcat agactttgac aattcatgac tttttttacc catctctatt
     8881 aaagtactat cgggcaatag tattattttt tttagtgcct ctttgagcga atcaacattg
     8941 cttggctcat ggtaatatcc gttctcacct tgttttaaaa atgcagttcc tgcaggcgct
     9001 tttgtactta acaacaacgg ataacctgca atggcatatt catgtactac tacaccccaa
     9061 tgctcatcgt aagttggtaa aagaaataca cctgtctggc gcatatactc ttccatagca
     9121 gaaggttgaa caaatcctac atttctgatt ttaggatgtt gcggaaattc tttttcaagt
     9181 ggacctttac caaggcacca caactcccat tcattaggca tttcattctg caactgcgta
     9241 aatgcctgcc atagttcgcg aatacctttt atttcgatat aacgtccaac aaaaataaaa
     9301 cgcttaggaa aattcttttc ttttataggc attatttttt gatatgcctg atcaaacaat
     9361 tcgtaattgg cagaatacat tcctgtttca atacgatttt ctttaaagcc aagttttttt
     9421 gcataaataa cctgcggctg acccggcacc cagcaatgtg aaaaaatgcg aggtaaaata
     9481 aatggagctg ttaccgatgc tacatactgt ttaagttttc cttcccacaa attatcgaag
     9541 gcaagtactg ttggtatttt tccggaatat tgtttagcga catttacata acctttatcc
     9601 atccatccat tacaaaaaat aaaatcagga tttatagaag caacaaggtc attaagcttc
     9661 tgctcctgat actgatttct gtcgtaataa gtaatgcttt ctacatcatc aaagcggaat
     9721 ggcgcattgg gatcaagcgg atattttaca atatgtaaat ccaatggata gtttagggct
     9781 gtctttttaa aacaagcttc aacataaccg gcaggttcaa gatacagaaa caaggctttc
     9841 ttcttcgcga tcggcattaa attgattata atgtatgatt aaattcaaaa ctaaaattaa
     9901 aataatatga aatggattta atgaacccat tatccaggct tcgaacgaag ctgaaaatat
     9961 tacagcaaat aaaattggga aagccaattt agaatgatgc attgctctga caaaagtttt
    10021 tataaaccct actgcaaaaa gaataagtcc tatcaaaccc gtgttcatcc ataaagcaag
    10081 ccatgagttg tgtacccctc cctgatgacc taatgcactt aaccaatgtc tgtttgcata
    10141 aaaataccat tcatcatagg caaagcctct acctaaaaaa aattgtgatt gaatttgttt
    10201 ccatgcagca cgccatgcaa ttaatctgcc tgagccatct tcaatagctt ctgcacgcaa
    10261 ataatgtcca agacccaatg cttctacaat agcaactata ttctgattga ttaattcata
    10321 agcaacaagt gccactacca gaattgcaaa tcctgcaagg taacccatct catagaagcg
    10381 agcaaaaagt aagaacaaaa tgattgacat cattgtgttt cgtgaaactg ccaacaacgt
    10441 acttaaaata attaggccgt atataagtct gacttcattc tggtcaagaa ggcccggaaa
    10501 cttttttaat gaaatgtaaa ctaatataaa caacagtgta cagccaatac caataccatt
    10561 gggattgcca taaacaccac taaatctacc cacaagaaac actgtgtttg ggaagagtgg
    10621 aatcataatt attcctgcca ataaaatgca acaaaagagc attattacgt cacgcaaaaa
    10681 actttcacca agctccctaa aagcttttaa aaaatatgcc ggaacaacag taaacagcag
    10741 tatgtaactt aatgtcttca tagcgctatt cataaggttt accgttggtg cttgcatcag
    10801 catagcaata ctccacaaga agaatggaat gaacggttta aaataatcat ttgtgcctgt
    10861 gaaatttttt ctgtcaaaaa aataaaagac tgaaagcaaa acgatataaa aattctttgc
    10921 ctgtgcggca tataaagctt cgaattgtct gctatcactc atgataagca gaaatatcaa
    10981 tccaagaata agttctttgt acatgccctt tattttgagc ataataacac ttaacacaac
    11041 tgcagcaaca ccaagagtct catttaccat accacagaaa acccatgtga aacacatgat
    11101 tacaaactgt gcattatttc tgataaactg aagcatttgg tggtttagaa ttacaaaatt
    11161 aacatttatg ttttctgttc agccatttgt tgaaagagct caagatgctg ttgtgcaata
    11221 acaggccagg taaatttctc ttttactcgt gaataagatt gttcagccat tcgtttcttt
    11281 gcctgttcgt tcaaaacaaa gtacattgaa at
//
LOCUS       DAOOOL010000080        11162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_535_length_11162_cov_61.294409, whole genome shotgun sequence.
ACCESSION   DAOOOL010000080 DAOOOL010000000
VERSION     DAOOOL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_535_length_11162_cov_61.294409"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKU82_07665"
     CDS             <1..709
                     /locus_tag="PKU82_07665"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOZ82786.1"
                     /translation="CLPRKPGMSRDDLIATNAGIVRSVTENIVKHSPNTIIIVVSNPL
                     DVMTYCAFLTSGFDSKRVFGMAGILDTARYRAFLAEALNCSPKDIHAVLMGGHGDTMV
                     PLPRYTTVSGIPVTEMIAADKLESIIHRTKVGGGELVNLMGTSAWYAPGAAAAQMVEA
                     IVRDQKRIFPCCAWLQGEYGQKDIYLGVPVKLGKKGIEQIIELKLNTEEKQLLDGSAK
                     AVKEVMNVLDGMQQPVK"
     gene            863..2041
                     /locus_tag="PKU82_07670"
     CDS             863..2041
                     /locus_tag="PKU82_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOZ82787.1"
                     /translation="MNIILADSNRNDLLPFTFVRPVAEIRCGILTITEKWNRLSGAQC
                     SWLTEKYLSAKYSLAKEKVNCVANASVLIDKALFNEIKTLKTGQALVKGNTVIAVCTE
                     QQQFETLEDVSYNEAFETGLEVNVLKYPWDIFRFNDKAINSDFQLLTEGRKSAGYDSS
                     NTVINEQQVFIEDGAKVMCSIINASTGPVYIGRDAEIMEGCMIRGPFALGEHATLKMG
                     AKIYGATTIGPHCKVGGEVNNSVIFGYSNKAHDGFLGNAVIAEWCNLGADTNNSNLKN
                     NYGMVKAYNYSKKDYINTGLQFCGLMMGDHSKAAINTMFNTGTVTGICANIFGSGFPP
                     KYIPSFAWGGFDDESVFQLDKALELAAEVYKRRELIFDKKEQDIINHVNMLELILPKN
                     "
     gene            2113..4593
                     /gene="lon"
                     /locus_tag="PKU82_07675"
     CDS             2113..4593
                     /gene="lon"
                     /locus_tag="PKU82_07675"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOZ82788.1"
                     /translation="MFNPEDIKHTLIGGINDDEPELIPLLSSEDEEQLNKESIPDELS
                     ILPLRNTVLFPGVVIPITVGRDKSIKLIKDAYKSNKIIGVVAQKDTNIEDPGFNDLNK
                     IGTVASVIKMLRLPDGNTTIIIQGKRRFSLLEMTQSEPYLRAKVEPLNDIVAALKEKE
                     FKATVDSLKDISLQIIQHSPQIPSEAGFAVRNIESPAFLINFISSNMQLEVSKKQDLL
                     EMTDIKQRALKVLEYLTQELQMLELKSQIQNKVKVDLDKQQREYFLNQQLKTIQEELG
                     GNTPDQELQQLKSRAKDKKWNKHVAEIFNKEIERLGRINPAAAEYSVTLNYLDLLLEL
                     PWGEFTEDNFNLKHAQKVLDKDHYGLEKVKTRILEYLAVLKLKKDMKSPIMCLYGPPG
                     VGKTSLGKSVAKALGRKYVRVSLGGLRDEAEIRGHRKTYIGAMPGRIIQNIKKAKSSN
                     PVFVLDEIDKLTNDFHGDPSSALLEVLDPEQNNAFYDNYIELDYDLSNVLFIATANSL
                     SSIQPALRDRMEIIEINGYTVEEKIQIAVKHLIPKQLEAHGLKKHHATFNNSVIEYII
                     EFYTSESGVRSLERNLASVIRSIAKRVALNEKYDKKIDKDTVEKALGAPIFLRDRFSD
                     NSVAGVVTGLAWTSVGGDILFIETSLSRGKGRLLLTGNLGEVMKESAQIALAYLKAHA
                     ADFGIDYRVFDRWDIHIHVPEGATPKDGPSAGVTMLTSLASAFTQRKVKLKLAMTGEI
                     TLRGKVLPVGGIKEKILAAKRAGITDIILCADNEKDIKDIKSEYLKGLKFHYVSKMGE
                     VIDIALLREKVKNPLNFTISQLERKETV"
     gene            complement(4597..5517)
                     /locus_tag="PKU82_07680"
     CDS             complement(4597..5517)
                     /locus_tag="PKU82_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOZ82789.1"
                     /translation="MKRRLLEYLSLLLNYTRRITLPGFQKIPIYDVGEFFLRELFKGD
                     LQIRARAIAFSFFLAIFPAIIFLFTLIPYVPIAGFQERLLSLLQSMLPYNTYDTAYKT
                     IEEIIVRQNGGLLSFGFILALIVSTNGVFALIEGFNKTIMHEKRKGWKQRLVALYLTV
                     LLATLLIIAILLIIITEVILHYFNNHILNLDQSSVYLLLAGKYLILILLCFTAISSLY
                     FFGPSGGSKKWHFFSPGSILATILIAVTSIAFNFFILNFGQYNKIYGSIGTLIVILIY
                     LNFNCIQLLIGFELNNAIEKAKFKPTVVEK"
     gene            complement(5514..6350)
                     /gene="nadC"
                     /locus_tag="PKU82_07685"
     CDS             complement(5514..6350)
                     /gene="nadC"
                     /locus_tag="PKU82_07685"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOZ82790.1"
                     /translation="MDLDQYISLSLQEDVGPGDYSALSCIPADAQSKANLIIKDKGIL
                     AGIELAEKIFKTVDASLQINIFKKDGQHVDKSEIAFTVEGSVLSILKTERLVLNCMQR
                     MSGIASTTYLYAEQVKHTKAKILDTRKTTPLNRYIEKWAVRIGGGHNHRFGLYDMIMI
                     KDNHVDYAGGIKQAIESANTFRKNRNLNIPIEIETRNLNEVEQVLNTGCVERIMLDNF
                     QPALIKEAVSMISGRFETEASGGIILQNVKDYAETGVDYISVGALTHSFKSMDMSLKA
                     SQ"
     gene            complement(6487..6876)
                     /locus_tag="PKU82_07690"
     CDS             complement(6487..6876)
                     /locus_tag="PKU82_07690"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOZ82791.1"
                     /translation="MKKTIKLLLSVMLIVTVVKAADVFDDIGTAIRSGDARQIARFFN
                     NNVDLTIFNQEEVYSKAQAEMVLKDFFSKNAPKSFTIIHKGVSKEGARYAIGTLTTTQ
                     GQNIRTYFFVKESGGNSSIQELRFEKE"
     gene            7081..7548
                     /locus_tag="PKU82_07695"
     CDS             7081..7548
                     /locus_tag="PKU82_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOZ82792.1"
                     /translation="MQIKLLMIGKNKDSFADAAIAEYASRVERFNSFEVKYLKEAKNN
                     KNLTAAMDAEAENIFKELSPNDFVILLDEKGKEYTSVQFATKIEQLRIKNFKQLVFIA
                     GSAYGFSEKVYARSNELFALSKMTFTHTHARLIFAEQIYRAFTILNNHPYHHI"
     gene            7623..9329
                     /gene="asnB"
                     /locus_tag="PKU82_07700"
     CDS             7623..9329
                     /gene="asnB"
                     /locus_tag="PKU82_07700"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ82793.1"
                     /translation="MCGIAGILNFQHKPVDLSVLKQMSDTLSHRGPDGEGIWHNEQKN
                     IGLAHRRLAIIDLSDEARQPMHYLGRYTIVYNGEIYNYLELKADLEKKGYQFVSHSDT
                     EVILAAFNHKREKCLEDFDGMFAFAIWDSKLQQLFLARDRFGEKPLRYYMDSERIVFA
                     SEVKALFKAEVPKKVSKEKMDNYLQNNISIYNTETFFDGIKKLPHGHYAFVQNGNIDI
                     REYYRLQYKNLVSMDDHEYAEEFKRLFFLSINRRLRSDVPVATSFSGGLDSSGIVCVM
                     HQLKEKVNYKLFSARFGDDVKDEGKWMKHITDLLHFPQINKQIEPQEILEDIEKIVYH
                     NEIPIGSTSVTAQYLLYKEVKNHHVKVILDGQGADEMLAGYGHYRFHYLNDLLLRFRV
                     AEYINERRAYNDAYQTSLPIGPWQLTKMFLTKTVKPFEDNTRGYQSFKATLYNDMMMS
                     LQLLLNFSDSNSMAHGVEVRLPFLYHKLVDFAFSLPANQIYRNATTKYVYRNAMNGII
                     PDAILNRKDKVGFAPPQERWLPQFKLPENARLRDMGLTYSDNHWRNYISEVFLKVADS
                     RY"
     gene            complement(9330..10088)
                     /locus_tag="PKU82_07705"
     CDS             complement(9330..10088)
                     /locus_tag="PKU82_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ82794.1"
                     /translation="MPSIFITGATAGFGRAIALKFAQHHWSIIINGRRTDRLRTLEDE
                     IKNLGADVISLPFDVRNEQQVNEAIENLPIKWKQIDALVNNAGLAAGVNLIQDGKTED
                     WNTMIDTNVKGLLYVTKNIIPLMIKQKSGHIINIGSIAGKDAYMKGNVYCATKYAVDA
                     LTKSMRIDLLEHGIKVSAVNPGAAETEFSLVRLKGDVEKANAVYKGFEPLVAEDIAEI
                     VYFVATRPAHVCINDVVVTPTAQANTAHLLRKTN"
     gene            complement(10095..>11162)
                     /locus_tag="PKU82_07710"
     CDS             complement(10095..>11162)
                     /locus_tag="PKU82_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOZ82795.1"
                     /translation="TAEECKNPQRDLPRGMMWAIIICTILYIAIALVLTGMVNYSTLA
                     VGDPLAYVFSVLNLKWMSAIIAVSAIVAMASVLLVFQLGQPRIWMSMSRDGLLPKKFA
                     TIHPKYKTPSFASIVTGFVVAVPALFMNLTMVTDLCSIGTLFAFVLVCAGVLKMQITP
                     NAPRGSFNTPYVNAKYIFPLMLILTAALSWSYNKDGVSNFLKNTPQPYSAENLIPSLG
                     ADRIKDLVNTVSLKDSAAFAKADNDLANYFSQITTDNYLQKLTDYGLSHKEIYESGLG
                     LFKHKIPTWIFIISCLYLCVLTWKHNLSLIPLLGLVCCLYMMSEMGISNWIGFSIWLA
                     VGLIIYFGYSYKNSKLNIIKK"
BASE COUNT         3650 a         1931 c         2160 g         3421 t
ORIGIN      
        1 atgtcttcca cgcaagccgg gcatgagtcg tgacgattta atcgctacca atgcaggtat
       61 cgttcgttct gtgactgaaa atattgttaa acattctcca aataccatca tcattgtagt
      121 ttccaatcct ttggatgtaa tgacctattg tgcattcttg acatcaggat ttgactctaa
      181 gagagttttc ggtatggcag gaattttaga tactgcacgt tacagagcat ttttagctga
      241 ggcattaaat tgttctccaa aagatattca tgctgtattg atgggtggtc atggtgatac
      301 tatggtgcct ttgccaagat atacaactgt ttcaggtatt cctgtaacag aaatgatagc
      361 tgctgataaa ttagaaagta ttatacatag aactaaagtt ggtggcggtg agttggtgaa
      421 ccttatgggt acatctgctt ggtatgcacc gggtgctgct gctgctcaaa tggtagaagc
      481 aattgttcgc gatcaaaaac gaattttccc atgttgtgca tggctgcaag gtgagtacgg
      541 acagaaagat atttatcttg gtgttccagt aaaattgggt aaaaaaggta ttgaacagat
      601 tattgaactt aaactcaata ctgaagaaaa gcaattactt gatggcagtg caaaagctgt
      661 aaaagaagtg atgaatgtac ttgatggtat gcaacaaccg gtaaagtaaa tttctttctg
      721 taaaataata aaatgccgct tgttgtcagg cggcatttgt tttttaataa ttacttaatc
      781 tgctgaactg atttttttgc tacattcgca gaagtacagt tctaaccatt gtaatattaa
      841 atttttaacc cgttaaatgt caatgaacat tatacttgct gatagcaacc gtaacgattt
      901 acttccattt acttttgtca gacctgtagc agaaattcgt tgtggtattt taaccattac
      961 agaaaaatgg aatcgcctga gtggagcaca atgctcatgg ctgactgaaa aatatctgtc
     1021 tgcaaagtat tcgttagcta aagaaaaagt taactgtgtt gccaatgcat ctgtacttat
     1081 tgacaaagcg cttttcaatg aaatcaaaac tctgaaaaca ggtcaggcac ttgttaaggg
     1141 aaatactgtc attgctgttt gtacagaaca acaacaattc gaaacattgg aagatgtaag
     1201 ctacaatgaa gcatttgaaa caggacttga agtgaatgtg ctgaaatatc cttgggatat
     1261 ttttcgattc aatgataaag ctatcaacag cgattttcaa ttacttacag aaggaagaaa
     1321 aagcgcagga tatgattctt caaataccgt aattaatgag cagcaggttt ttattgaaga
     1381 tggtgccaag gtcatgtgca gtatcatcaa cgcttcaaca ggccctgttt atattggtcg
     1441 tgatgctgaa ataatggaag ggtgcatgat aagaggccct tttgcacttg gtgaacatgc
     1501 tacacttaaa atgggtgcta aaatttatgg tgccactaca attgggccac actgtaaagt
     1561 gggaggcgaa gtaaacaatt ctgttatttt tggttattcc aataaggcac atgatggttt
     1621 tcttggtaat gcagtaattg ctgagtggtg taatcttggt gctgatacca ataactcaaa
     1681 cctgaaaaat aattatggta tggtaaaagc atataattat tcaaagaagg attatatcaa
     1741 tacaggttta caattctgcg gacttatgat gggcgatcat agcaaggcgg caatcaatac
     1801 catgtttaat acaggcacag taacaggaat ttgtgctaat atttttggaa gtgggtttcc
     1861 tcctaaatat attccctctt ttgcctgggg aggatttgat gatgaatcgg tattccaact
     1921 tgacaaagca ttggagttag ctgccgaggt atataaaaga agagaattga tttttgacaa
     1981 aaaagaacaa gatataatta atcatgtcaa catgcttgag ctaattctgc caaaaaatta
     2041 aatggcacaa gcattgacag attactattt taaactaata acagcctgtt ggctgacaac
     2101 ttgacgtaaa ctatgttcaa tccggaagat ataaaacaca cactcattgg cggcataaat
     2161 gacgatgagc ccgagttaat accattactt tcttcagaag atgaagaaca actcaataag
     2221 gaaagcatac ctgacgaatt gtcaatttta ccattgcgga atacagtact ttttccaggt
     2281 gtggttattc ccattacggt agggcgcgat aagtcaatta agctgataaa agatgcttat
     2341 aaaagcaata aaattattgg tgtggttgca cagaaggaca caaacattga agatcccgga
     2401 ttcaatgacc tgaataaaat aggaactgtt gccagtgtca taaagatgtt gcgattacct
     2461 gatggtaaca ctacaattat cattcaggga aaaagaagat tttctttact tgaaatgaca
     2521 cagagcgagc cttatctgag agctaaagtt gaaccactga acgatattgt tgctgcattg
     2581 aaagaaaaag agtttaaagc aacagttgat tcgctcaaag acatttcctt acaaatcatt
     2641 caacattctc cacaaattcc aagtgaggcc ggatttgcag ttcgcaatat tgagagccct
     2701 gcttttttaa taaattttat ttcgagcaac atgcaattgg aagtttccaa gaagcaagac
     2761 ttgctcgaaa tgacagatat aaaacaacgt gctttaaaag tacttgagta tctcacacaa
     2821 gagttgcaga tgcttgagct aaaatcgcag attcagaata aagtgaaagt tgatttggat
     2881 aagcagcaac gcgaatattt tttgaatcaa caattaaaaa ctattcagga agaattgggc
     2941 ggcaacacac cggatcagga attgcagcag ttaaagtctc gtgccaagga taaaaaatgg
     3001 aataagcatg tagcagaaat tttcaataaa gaaattgaaa gattgggacg cattaacccg
     3061 gcagcagcag aatacagtgt gactttaaat tatctcgatt tattactcga attgccctgg
     3121 ggcgaattca cagaagataa tttcaatcta aaacatgcac aaaaagtact tgacaaagac
     3181 cattacggat tagagaaagt aaaaacgcgc attctcgaat atcttgcagt gttgaagctg
     3241 aaaaaagaca tgaagtcgcc aataatgtgt ctttatggtc ctccgggagt tggaaaaacc
     3301 tctcttggta aatctgtagc caaggctttg ggcagaaagt atgtacgcgt ttctctcggt
     3361 ggattgcgtg atgaagcaga aataagagga catcgtaaga cctatatcgg tgcaatgccc
     3421 ggcagaatta ttcagaatat caaaaaggca aaatcatcaa accctgtttt tgttcttgat
     3481 gaaattgata aactcaccaa cgattttcat ggtgacccaa gttctgcatt actggaagtt
     3541 ttagatcctg aacagaacaa tgcattttat gataattaca ttgagcttga ttatgactta
     3601 tcgaatgtgt tgtttattgc tactgccaac agtttgagct ccattcagcc tgcactgcgc
     3661 gacaggatgg agataattga aatcaacggc tacacagttg aagaaaaaat tcagattgca
     3721 gtaaagcacc tcatcccaaa acagttggaa gcacatggat taaagaaaca ccatgctact
     3781 ttcaataaca gtgtaattga atacataatt gaattttata ccagcgaaag cggtgttcgt
     3841 agtcttgaac gcaatctggc atcagtcatc agaagtattg caaagagagt tgccttaaat
     3901 gaaaagtatg ataagaagat agataaagat actgtagaga aagcacttgg tgcccctata
     3961 ttcttgcgcg acagattttc agataacagt gttgccggag ttgtcacagg tttggcatgg
     4021 acatcagtag gcggtgatat tttatttatt gaaaccagtt taagcagagg caaaggacgc
     4081 ttattactta caggaaattt gggagaggtc atgaaagaat ctgcacagat tgctttagct
     4141 tatttgaagg cacatgcagc agattttggc attgattatc gcgtgtttga tagatgggat
     4201 attcacattc acgttcctga aggagcaact cctaaagatg gaccctctgc aggtgtcaca
     4261 atgctcactt ctcttgcatc agcatttacc caacgtaaag ttaagttgaa attagctatg
     4321 accggtgaaa ttactttgcg cggaaaagta ctgcctgttg gtggaattaa agaaaaaatt
     4381 cttgcagcaa agcgcgcagg aataacagac ataattcttt gtgccgacaa tgagaaagat
     4441 attaaagaca tcaagagcga atatcttaaa ggactgaaat ttcattacgt aagtaaaatg
     4501 ggtgaggtaa ttgacattgc attgcttcgc gaaaaagtga aaaacccatt gaactttact
     4561 atcagtcagc tcgaaagaaa agaaaccgtt taagatttat ttttctacta cagtgggctt
     4621 gaattttgct ttttcaatgg cattattaag ttcaaatcca ataagcaact gtatgcagtt
     4681 aaagttcaga taaattagaa ttactatcag cgttcctata gaaccataaa ttttattgta
     4741 ctgtccgaaa ttcagaataa aaaagttgaa tgcaatggat gtaacagcta taaggatagt
     4801 tgccagaata cttccgggtg aaaagaaatg ccatttttta ctgccaccgg aaggaccaaa
     4861 aaagtagagt gatgatatgg ctgtgaaaca aagcaaaatc agaataagat atttgcctgc
     4921 gagcaacaaa taaactgagc tctgatctaa gtttagaatg tgattattga aataatgcag
     4981 aataacttct gtgatgataa ttagtaatat tgctatgatt aataatgtcg ctaacagaac
     5041 agttagatat aatgcaacta atctttgttt ccatccttta cgtttttcgt gcatgatggt
     5101 tttgttgaaa ccttctatca atgcaaacac accattggta gaaactatta atgccagaat
     5161 aaaaccaaat gaaagcaggc ctccgttttg tctgacaata atttcttcta ttgtcttata
     5221 tgcagtgtca taagtgttgt agggaagcat agattgtaac agacttaaca gacgttcctg
     5281 aaagcctgca atcggtacgt aaggaatcag tgtaaaaaga aaaatgattg caggaaagat
     5341 ggctaaaaaa aatgaaaagg caattgctct ggctcttatt tgtaaatcgc ctttaaataa
     5401 ttcacgaaga aaaaattcac ccacgtcata aattggaatt ttttgaaagc cgggtagcgt
     5461 aattctacgt gtgtaattta acagtagtga taagtattcg agcaacctgc gtttcattgg
     5521 cttgccttta aactcatgtc catactttta aaagaatgtg ttaatgcgcc aacagaaata
     5581 tagtcaactc ctgtttcagc ataatctttt acgttctgaa gtataatgcc accggaagct
     5641 tctgtttcaa aacgacctga aatcatggac acggcttctt tgattaatgc aggttgaaag
     5701 ttgtcgagca ttattctttc aacgcaacct gtgtttagca cctgttcaac ttcgtttaag
     5761 ttgcgtgttt ctatttcaat aggaatattc aggtttctgt tttttctgaa agtatttgct
     5821 gattcaatag cttgttttat tccacctgca taatcaacat gattatcttt aatcataatc
     5881 atgtcataca atccaaagcg atgattgtgc ccaccgccaa tacgaacagc ccatttttct
     5941 atgtaacgat tcagtggtgt tgtttttctg gtatctaaaa tttttgcttt tgtatgtttc
     6001 acctgttctg catataggta tgtagtggag gcaatgccgc tcatacgctg catacagtta
     6061 agtaccaaac gttctgtctt cagtatagaa agaacgcttc cttcaactgt aaaagcaatt
     6121 tcacttttat caacgtgttg cccatctttt ttaaagatgt tgatttgcaa cgaagcgtca
     6181 acagttttaa atattttttc tgcaagttca atgcctgcaa gtattccttt atctttaata
     6241 ataagattgg ccttgctttg agcatctgcc ggaatgcaac ttaaagcact gtaatcaccg
     6301 ggtccgacat cttcctgtag tgataatgaa atgtattgat ctaaatccat gataattatt
     6361 tctgctgcaa aattcagctt tttgattcaa agaatcatgt aggatacgat ttttttaaaa
     6421 tactaaaaag gaaaaagccg taaacaagag tgaatacggc tttcaaaaac tatggggaag
     6481 atattcttat tctttttcga agcgcaattc ctgaatagaa gaattgccac cactttcttt
     6541 tacaaagaaa taagttctga tattctgacc ttgcgttgta gtgagtgttc ctattgcata
     6601 acgggcacct tccttcgaaa cgcctttatg gattatggtg aaactttttg gtgcgttttt
     6661 actgaaaaaa tctttaagca ccatttctgc ctgcgcttta ctgtaaactt cttcctgatt
     6721 aaatattgtc aaatcaacat tgttattaaa gaaacgagca atttgtctgg catcgccaga
     6781 acggatggca gtgccgatat catcgaaaac atcagctgcc tttacaacgg taactatcag
     6841 catgacagaa agaagaagct tgatggtttt tttcatcctt gataaattat gtttgtgaac
     6901 tgacaaaaat caagccaaga gcgcatagga atgaaaaact tatgaatgca ttttagcata
     6961 aatcttacag taataattaa acacttaaat gtaaatccga aacaatatta aggcataaaa
     7021 atttactttc aaaacttaat ttcagatttt tgtaaataat cattattgga taacgtaatt
     7081 atgcagatca aattgttaat gataggtaag aataaggata gttttgccga tgccgcaata
     7141 gctgagtatg cgagtagggt tgagaggttt aacagttttg aagtcaagta tctgaaagaa
     7201 gccaaaaaca acaaaaacct tacagcagca atggatgctg aggctgaaaa tattttcaag
     7261 gagttatcgc caaatgactt tgttattctg ctggacgaaa aaggcaaaga atacacatcg
     7321 gtgcagtttg caacaaaaat cgagcagtta aggattaaaa atttcaaaca attggttttt
     7381 attgctggta gtgcttatgg tttttcggaa aaagtttatg cacgaagtaa tgaactgttt
     7441 gccctgtcaa aaatgacatt cacccatact catgcaagat tgatttttgc cgaacagatt
     7501 tacagagcat ttactatcct gaataatcat ccttatcatc atatttaatt tcatcggcat
     7561 actatttggt aagatggaaa atctaatttt gcactttcaa aaatttttga tcagaaatct
     7621 atatgtgcgg tatagccggc attctcaatt ttcagcataa acctgttgat ttatcagtat
     7681 taaagcaaat gtcagacacc ctttctcaca gggggcctga tggtgaaggt atttggcata
     7741 acgaacagaa aaatatagga cttgcacaca gacgattggc gattattgac ctttctgatg
     7801 aagccaggca gccaatgcat tatttaggtc gttatacaat agtctataat ggagaaattt
     7861 ataattacct tgaactgaag gctgaccttg aaaagaaagg ctatcagttt gtgtcgcact
     7921 ccgacactga ggtgattctt gcagcattta accataagcg ggaaaagtgc cttgaagatt
     7981 ttgacggtat gtttgccttt gcaatatggg acagtaaatt gcagcagtta tttttagccc
     8041 gtgaccgttt tggagaaaaa cctttacgtt actatatgga tagtgaacgg atagtttttg
     8101 cttcggaagt aaaagcattg tttaaagccg aagttccgaa gaaggtttct aaagagaaga
     8161 tggataacta ccttcagaat aatatttcaa tttataacac tgaaacattt tttgatggaa
     8221 taaagaaact gccacatggt cattatgctt ttgtgcagaa tggcaatatt gatatccgtg
     8281 agtattacag attgcaatac aagaatcttg tatccatgga tgaccatgaa tatgcagagg
     8341 agttcaaaag gttgtttttt ttatcaataa acagacgatt gcgaagtgac gttcctgtgg
     8401 caacatcatt ttccggaggg cttgattcct ccggtattgt ttgtgtaatg catcaactca
     8461 aggaaaaagt taattataaa ttattttcag cacgatttgg agatgatgta aaagatgaag
     8521 gtaaatggat gaagcatata acggatttac ttcactttcc acagataaat aaacagattg
     8581 agccccaaga aattttagaa gatattgaaa aaattgtcta tcataatgag attcccattg
     8641 gatccacatc agtaacagca caatatctgc tttataagga agttaaaaat catcatgtta
     8701 aagttattct tgatggacaa ggtgccgatg aaatgctggc agggtatggg cattaccgtt
     8761 tccattatct aaacgatttg ttgcttcgct tcagggtagc ggaatatatt aatgaaaggc
     8821 gagcatataa tgatgcatat caaacttcac tcccaattgg cccttggcaa ctgacaaaga
     8881 tgtttctcac aaaaactgta aagccatttg aagataacac tcggggatat cagtcattta
     8941 aagctacact ttataatgat atgatgatga gtcttcaatt gttgttaaac ttctctgata
     9001 gtaattctat ggctcacggt gtggaagtaa ggcttccatt tttgtatcat aagttggttg
     9061 actttgcctt ttcactacct gccaatcaga tttacagaaa tgcaacaaca aagtatgttt
     9121 accgcaatgc tatgaatgga attattcctg atgctattct caatcgtaaa gataaggtag
     9181 ggtttgcacc acctcaggaa cgatggcttc ctcaatttaa actcccggaa aatgcaagac
     9241 ttcgtgacat gggtttaact tattctgata accactggcg caattacatc tcagaagtat
     9301 ttttaaaagt agccgacagc cgatactgat taattggttt ttcgcaataa atgtgctgta
     9361 tttgcttgcg cagttggtgt aactacaaca tcatttatac aaacatgtgc aggacgggtt
     9421 gcaacaaaat atacaatctc tgcaatatct tccgccacta atggttcaaa tcctttataa
     9481 acagcattgg ctttttcaac atcacctttc aatctaacca aagaaaattc tgtttctgca
     9541 gcacctggat ttactgcact gacttttatg ccatgttcta ataaatcaat acgcatactt
     9601 tttgtcaaag catctactgc atactttgtg gcgcaataaa cattgccttt catataagca
     9661 tcttttccgg caatagaacc tatgttgatg atatgtccgc ttttttgttt tatcatcagt
     9721 ggaatgatat ttttagtgac ataaagcagt cctttcacat tagtatcaat cattgtgttc
     9781 cagtcttcag tcttgccatc ctgaatcaga tttactcctg ctgcaagtcc tgcattatta
     9841 actaatgcat caatttgttt ccatttgatg gggagatttt caatagcttc attaacctgt
     9901 tgttcgtttc gaacatcaaa aggaagactg atgacatctg ctccaagatt ctttatttca
     9961 tcttctaaag tgcgaagtct gtcagttctt ctaccattaa taataatgct ccaatggtgt
    10021 tgtgcaaatt ttaatgctat ggctcttcca aatcctgctg ttgcaccggt aataaaaatt
    10081 gaaggcataa taatttattt ttttataata tttaacttac tgtttttata gctgtaaccg
    10141 aaataaataa tcagccctac agccagccaa atcgaaaacc caatccagtt tgatatgccc
    10201 atttcactca tcatgtatag gcagcaaacc aaacctaata atggaatgag cgatagattg
    10261 tgtttccagg ttaaaacaca aagatacaga catgaaatga taaatatcca tgtaggtatt
    10321 ttatgtttaa aaagtccaag acccgattca taaatttctt tatgcgacaa tccgtagtcc
    10381 gttagtttct ggagataatt atcggtagtt atttgtgaaa aataatttgc aaggtcgttg
    10441 tcggctttag caaatgctgc tgagtctttc aaagaaacag tgtttaccag atctttaatt
    10501 ctgtcagctc ctaaagaagg aatcagattt tcggcagagt agggttgtgg agtgttcttc
    10561 aaaaaattgg aaacgccatc tttattatat gaccacgaca atgctgcagt taaaatgagc
    10621 attaacggga aaatatattt agcatttaca taaggtgtat taaaactacc tcggggcgca
    10681 ttaggcgtga tttgcatttt caaaactccg gcacaaacta acacaaaggc aaataaggtt
    10741 ccaatgctac aaaggtctgt aaccattgta aggttcatga aaagtgctgg cactgcaact
    10801 acaaagccgg ttacaattga tgcaaacgat ggtgttttat attttgggtg aatggttgca
    10861 aatttcttag gtagcaaacc atctctgctc atactcatcc agatgcgtgg ttgacccaat
    10921 tgaaaaacca acaatacact tgccattgca acaatagcac tcactgcaat aattgccgac
    10981 atccatttta aattcaatac actgaaaaca taagccaatg ggtcaccaac agccaaagtg
    11041 ctgtagttta ccattcctgt aagcactaat gctatggcaa tataaagtat tgtacagatt
    11101 attattgccc acatcattcc tcgaggtaaa tcacgttgtg gatttttaca ttcttctgct
    11161 gt
//
LOCUS       DAOOOL010000081        10935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_548_length_10935_cov_96.130515, whole genome shotgun sequence.
ACCESSION   DAOOOL010000081 DAOOOL010000000
VERSION     DAOOOL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_548_length_10935_cov_96.130515"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..841
                     /locus_tag="PKU82_07715"
     CDS             341..841
                     /locus_tag="PKU82_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOZ82796.1"
                     /translation="MSKYNLHYAGLKEGIHEFDYLLNQDFFDMFEHPIVQGAHVLIHL
                     TVNKSTTIVVLEIKAKGTVHVQCHRCLEEFEMPLDISKAIIVKTSGEDKEVDDDNIIL
                     ADNAHDIHLAQHFYDVVSLAIPIKVTHPDNGCNEETLRTLEKYMVKNENTNDPRWDIL
                     KNVSNN"
     gene            861..1046
                     /gene="rpmF"
                     /locus_tag="PKU82_07720"
     CDS             861..1046
                     /gene="rpmF"
                     /locus_tag="PKU82_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOZ82797.1"
                     /translation="MPNPKHKHSKSRRDKRRTHYKAEAPTIAICPTTGETHLYHRAYW
                     HEGKMYYKGKVVLEKAV"
     gene            1065..2030
                     /gene="plsX"
                     /locus_tag="PKU82_07725"
     CDS             1065..2030
                     /gene="plsX"
                     /locus_tag="PKU82_07725"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOZ82798.1"
                     /translation="MRIGVDIMGGDYAPLETTLGAIAALNDLSPQEVHLVLIGDEKKI
                     TEIIEGQGVNPKSFTIVNATEVIEMSDSATRAFSQKPNSGMAIGFGLLKQNKIDAFAS
                     AGNSGAMLVGSMFSVKAINGIQRPAISTILPQEDGSTGILLDVGANADCKPEVLQQFG
                     LLGSLLAEHVYHIASPRVALMNIGEEEEKGNLLSQQAHQLLKNTKEINFIGNVEGRDL
                     FNNKADVIVCDGFTGNVMLKEAESLYTLLKKRGIKDAYFDRFDYENYGGTPVLGVNGN
                     VIIAHGISKSKAIKNMILLAKEVTEAHLAEKIATFFQNNVVNQTL"
     gene            2040..3035
                     /locus_tag="PKU82_07730"
     CDS             2040..3035
                     /locus_tag="PKU82_07730"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOZ82799.1"
                     /translation="MEKIRAAITAVHAWVPDYVLTNKELETMVETNDEWIQTRTGIKE
                     RRILKDAGEGTSKIGVESVNGLLKKRGIQAEEIDLLICATTTPDLVFPATANIICDKV
                     GAKNAFGYDIQAACSGFLFALTTASKFVESGQYKKVVVVGADKMSAIIDYSDRATCII
                     FGDGGGAVLLEPDTNGYGIYDAVLKSDGSGRHFLHQKAGGSVKPPTHATIDAKEHFVY
                     QEGQTVFKFAVKGMADVSAEIMERNNLSADDIAWLVPHQANKRIIDATANRMGIGMEK
                     VMLNIQKYGNTTNGTLPLCLWEWEKQLHKGDNLILAAFGGGFTWGAVYLKWAYNS"
     gene            3382..4497
                     /locus_tag="PKU82_07735"
     CDS             3382..4497
                     /locus_tag="PKU82_07735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82800.1"
                     /translation="MRVLHFIWSANFGGIEKLVITLAQQQQLNENIKVSLLIGIRKGN
                     FLEMIEQAGLHCEFADMKSGLDFSSSKINRLTKLMSSYDVLHIHTFNPAIAYAAIKSK
                     IKIVFTVHGNFGFGRKRTIKDKVLQFLSRYFIRNHVNHITYNSEFCRNIAMEFYKVKK
                     STPATLIYNAIPNIELPIQDENDPAIKIKSSDFIVGTASRFAGNKRIDRLIEAFEIFS
                     KNKQDTTLLLTGDGVKMQELKNIVKIKKLENQVVFTGFKKNVFAWFNKMNVCVFPFEN
                     EAFGIVAIEALMLGKTVIVFSDGGGITEVIKPLNRLNIVESTAHLVDRLNFYYDNRNV
                     LADEQNNNRQYASRFNISSNEKAYFDVYKEVLNNTLE"
     gene            complement(4742..6796)
                     /locus_tag="PKU82_07740"
     CDS             complement(4742..6796)
                     /locus_tag="PKU82_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOZ82801.1"
                     /translation="MTNKYSLFFSFVLFLIHIRIQAQNVGINNPTPDASALLDMVSTD
                     KGILIPRMTTTLRITIAAPATGLLVYDSDLNKFYFFNGIAWIAVGSNIGWSLTGDAGT
                     ASGTNFLGTTDNQPLLIKVNNTKAGFIDQQEGTFLGRNVFLGKLAGFNNTITGFDNTF
                     IGAKAGEANTIGTLNTAVGAKSLQNAVATFSNTAIGRETMQQCISCVHSTAVGAGALQ
                     NIEGGFWNNAFGYLAMRQAANPGISNNAFGAETLFNNTGSDNCAFGHTALRQNTSGTY
                     NIAFGSLSLYENNTGSYNIGMGAFALQNNSTGSYNTVLGYKCAADGLSNNRVVAIGDS
                     TLYFNKASGIVAVGSRALRSNNGGTSNTAVGYESLYTNTIGNSNTALGDFSLKLNIVG
                     NANTAIGSLALQNNNGNNNTAVGSGAQSANTLGKDNSSVGALTLTNNLSGNFNIAIGS
                     NSLAQNISSHNNVGVGYNTLSAYNASGNTGNNTAIGTGAGDSYTSYSNCTFVGYQADA
                     NAASFSNSTAIGNGAIATASQQVKIGNATVTAIGGAVNWSIISDGRFKTNVKNNIPGL
                     NFINRLNPVSYNYDIKSYCSFIGTDEHSFNDDAAIQQKEKIIYSGFIAQEVEEAAKNS
                     GYNFSGIHKPENTNDTYTLSYADFVVPLVKAVQELTIQNVNLLKKIELLEAKYDLHKK
                     QD"
     gene            complement(6925..7566)
                     /locus_tag="PKU82_07745"
     CDS             complement(6925..7566)
                     /locus_tag="PKU82_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82802.1"
                     /translation="MKKIKIILVEDDKEIRELAEAILGSEKDMEVIKTFSNGENFLKS
                     WENFDCNVVVMDINMPGKTGIECVAEAKPKKPSVQFLISTVFENPQYIFQALCNGATG
                     YVLKNNTGDKLVSAVREIASGGSPMSASIARLVVDSFSHKQTTVINNEILTSREKEVL
                     DLLAQGLMYKEIAAKKEIAVETVRKHVYNIYEKLQVDSRTAAINKVYGKHPES"
     gene            complement(7594..9513)
                     /locus_tag="PKU82_07750"
     CDS             complement(7594..9513)
                     /locus_tag="PKU82_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ82803.1"
                     /translation="MIKTHYSFALSFVISFVFLFGISNAQSNSYDSLSKAVNTFQPAK
                     KFELADTIYLKNIEQLNKLFFEVGESDSMRNYALKGLMFVKKQKAAATEETMLESLSN
                     FEMVFNRQAGISYFDKGDYTNQLKYFQQFLVCAQNINSIKDIATAYVYIATCHRELED
                     LEKSFDYAKQAVQILKGDSFPSILGSAYALSSNYYIEEKINNDSALYYRKQAYLLFKK
                     ANNPMLLTSATFDMIDFFHSIRQTDSCKKYLAEIEPVIQELENPEQTMVFNTLSAQVK
                     LSEGKVANAIKMLRKARAIADKTDELNDNSQINRVLALALAADRKIAVAYNVMDSAFD
                     EYSEDLNTEKVRSLTQAQMNFEFEKERTIAAIEIKRQKQQHYFLIAIASVLLVFAGFV
                     FQRYRERHRSSLILAEKNKTIEKAYRELKETQHNLVETEKQREAQSIRVRIARDIHDE
                     IGSGLTKITLLSDVAKKKSNQEEVAEALSKITTYSKGVSSSLSEIVWAINPGHDNIAS
                     LINYMKSTANNLLEDSGVNYSLHFPDTEISSSIHPEIKRNIFLVMKEAINNSLKYANA
                     KNITVTFTLNKSNFKLIVADDGNGFDVTDFNKIIRNGNGLINMQQRMFQHQNKLTIIS
                     TPGHGCKIVAEGKQI"
     gene            complement(9528..9791)
                     /locus_tag="PKU82_07755"
     CDS             complement(9528..9791)
                     /locus_tag="PKU82_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82804.1"
                     /translation="MKEAINNSLKYANAKNITILFLIKDNQFNMEIADDGIGFNMDLL
                     EESGIKGNGLVNMRNRMMQHNCSLQIVTSENEGCKIIAQSKLY"
     gene            complement(9794..10096)
                     /locus_tag="PKU82_07760"
     CDS             complement(9794..10096)
                     /locus_tag="PKU82_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82805.1"
                     /translation="MYYQLKMFDTSISIKDSVMHQPNEYFPENLAKLNRLKAQNFFRI
                     QDFSTALMLYKKAFLINYMKSTSNNLLEDSGVNYSLHFPDTVISSSIHPEIKRTFF"
     gene            complement(10364..>10935)
                     /locus_tag="PKU82_07765"
     CDS             complement(10364..>10935)
                     /locus_tag="PKU82_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOZ82806.1"
                     /translation="SDDVEVTNLNRQILFSENNIGESKVHAAANRLKELNLQCKINLL
                     QKEIHSQNEFEVMLNECNKVDYIVLSADKPIDLVLWASALCKQYQFKYLKCGYMAYQG
                     LIGPLLGYNTKTYEEIFQSWAKDIHSQNELIKIQNNKHMAPSMAASNAIFANIATWEM
                     IKDITGICKSVLTEQRILFNLKTMDMVYG"
BASE COUNT         3474 a         1907 c         1887 g         3667 t
ORIGIN      
        1 tgactataca atcattttcg gcttaaaaaa tgcttgactc aacaatgtta acattaattg
       61 ggtattttac catgcttttt tgtaccaatg gtaatttgaa tatgttaatg gaaaatattt
      121 gttttatttc cagaagatat tgccaattcc tactgcaaaa taccgtcaat tacatgcagt
      181 aaaccaatct ttaccgacat attttgttaa tacttaaatg gttctatctc ctgacaatca
      241 atagccctta ataataatta acaaaaacaa ggtgttggaa tagcgatttt ttatactttt
      301 gcccattcgt tcccaaaata ggaagtacgt agtggaaaag atgtcgaaat ataacctgca
      361 ttatgccggt ttaaaagagg ggatacatga gtttgattac ttactgaatc aggacttttt
      421 cgacatgttt gaacacccta ttgttcaggg agcacatgtt cttattcatc ttaccgtaaa
      481 caaaagcacc accattgtgg ttctggaaat taaagctaaa ggcaccgtac acgtacagtg
      541 ccatcgttgt cttgaagagt ttgagatgcc tttggatata agcaaggcta ttatcgttaa
      601 aacctctggc gaagacaaag aagtggatga tgacaacatc attttagccg acaatgccca
      661 cgatattcat ctggcacagc atttttatga tgtagtttca ttagctatac ccataaaagt
      721 gacacatcct gacaatggct gcaatgaaga aacccttcgt acacttgaaa agtatatggt
      781 gaagaatgaa aacacaaacg acccgcgatg ggatatttta aaaaatgtaa gtaacaattg
      841 atattaaata attaaacgtc atgccaaatc caaaacacaa acactccaag tcgagaagag
      901 ataagcgcag aacacattac aaggcagaag ctccaacaat tgccatttgt cctaccacag
      961 gcgaaacaca tttatatcat cgtgcctact ggcatgaggg caaaatgtac tacaaaggca
     1021 aggtggtttt agaaaaagct gtttaatcgc tgtattcctt tatgataagg ataggagttg
     1081 atattatggg tggagattat gctccactcg aaactacctt aggtgcaatt gcagcattaa
     1141 atgacctttc gcctcaggaa gtacacctgg ttctaatagg tgatgagaaa aaaataacag
     1201 agataattga agggcaggga gtcaacccta agtcgtttac aattgtaaac gctacagagg
     1261 tgattgaaat gtctgacagt gcaacacgtg ctttctctca gaaacccaac tcaggtatgg
     1321 ccattggttt tggcctgcta aaacaaaaca agattgatgc ctttgccagt gccggaaatt
     1381 ccggtgctat gttggttggc agcatgttca gcgttaaagc cattaatggt attcagcgtc
     1441 ctgctatctc aaccatttta ccacaggaag acggctcaac aggaatttta cttgatgttg
     1501 gtgccaatgc cgactgtaag cccgaagtgt tgcaacaatt tggattactc ggttcgctgc
     1561 ttgcagaaca tgtttatcat attgcatcac cacgtgtggc tttgatgaat attggtgaag
     1621 aggaagaaaa aggaaacctg ctttcgcaac aggcacatca gttgcttaaa aacactaaag
     1681 aaataaattt tatcggtaac gttgaagggc gcgacttgtt caacaacaaa gctgatgtca
     1741 ttgtatgcga tggcttcaca ggtaatgtaa tgcttaaaga agccgaatca ctttatacgc
     1801 tacttaaaaa acgcggtatt aaagatgcat acttcgaccg ctttgattat gaaaattatg
     1861 gtggcacacc ggtgcttggt gtaaacggaa atgtaattat tgcacatggc atttcaaaat
     1921 cgaaggccat caaaaacatg atattgcttg ccaaagaagt taccgaagcc catcttgcag
     1981 aaaaaatcgc aacttttttt caaaacaacg tagttaatca aacactataa ttttttttga
     2041 tggaaaaaat cagagctgcc atcaccgctg ttcatgcctg ggtgccggac tatgttctta
     2101 caaacaaaga actggagacc atggttgaaa ccaacgatga gtggattcaa acaagaacag
     2161 gaattaagga acgcagaata ttaaaagatg ccggtgaagg tacttccaaa attggtgttg
     2221 aatcggtaaa cgggctatta aaaaagcgtg gcatacaggc agaagaaatt gacttgctga
     2281 tttgtgccac tactactcct gatttggttt ttccggccac agcaaatatt atttgtgata
     2341 aagtaggtgc taaaaatgct tttggctacg atattcaggc agcctgttca ggatttttat
     2401 ttgcattgac tactgcttca aaatttgttg aaagcggaca gtataaaaaa gttgttgttg
     2461 tgggtgccga taaaatgtcg gcaatcattg actacagcga ccgtgccacc tgcattattt
     2521 ttggtgatgg tggtggtgct gtattattag aaccggatac caacggttac ggcatttacg
     2581 atgctgtttt aaaaagcgat ggcagtggac gacattttct gcatcagaaa gcaggtggta
     2641 gtgtgaaacc tccaactcat gcaactattg atgccaaaga acattttgtc tatcaggagg
     2701 ggcaaacggt ttttaagttt gctgttaaag gtatggctga tgtaagtgca gaaattatgg
     2761 aacgtaacaa cctgagtgca gatgatattg catggcttgt tccacatcag gcaaacaaaa
     2821 gaattattga tgctactgcc aaccgaatgg gcattggcat ggaaaaagtg atgctcaaca
     2881 ttcagaagta tggaaataca accaatggca cattaccact ctgtttatgg gaatgggaaa
     2941 aacaattgca caagggtgat aacctgattc ttgccgcttt tggcggaggc tttacttggg
     3001 gagcagttta tcttaaatgg gcttataaca gctaaggaat tcatattctt agaaaatacc
     3061 aaccgcatta ttcaattccg aaaaactctt tatttcactc ttaaaaaagt ttaaattttt
     3121 agaggtgctt tgaagaaaat tacaacaaaa ataaggttgt aatgaatggt ggtgaaaata
     3181 taaaagcaat ctatggtaaa cgagcgtctg caatacccat tcgattaata aaagtcgcta
     3241 cttgtctttt ttaatttctg ttgaacaaaa tcgagtgaaa gtttactttt gccttttatc
     3301 tgattcggtt attaatttta gtttttttaa ttattaaaca ggagaagaga cttgtttatg
     3361 attaactaaa tatcttaaaa aatgagagtg ttacatttta tttggtctgc aaattttggt
     3421 ggtatagaga agttggtaat cactcttgcg caacaacaac agttaaatga aaatataaag
     3481 gtttcgttgc tgataggaat cagaaaggga aattttcttg aaatgataga gcaggcaggt
     3541 ctgcactgtg aatttgctga tatgaaatca ggtctggatt tttccagtag taaaattaac
     3601 cgactcacaa aactcatgtc atcatatgat gtgctgcata ttcatacttt caacccggct
     3661 atagcctatg cggcaattaa atcaaaaata aaaatagtct ttactgttca tgggaacttt
     3721 gggtttggcc gcaagcgtac aataaaggat aaagtattac aatttctaag cagatatttt
     3781 atccgtaatc atgtaaatca cattacttac aactctgaat tttgccgtaa cattgcaatg
     3841 gagttttata aagtaaaaaa atcaacacct gcaacgctaa tctacaatgc aattccaaat
     3901 attgaattac ccattcagga tgaaaacgat ccagcgataa agataaaatc ttcagatttt
     3961 attgtaggga cagcctctcg ttttgcaggt aataaacgaa ttgatagact tatagaagca
     4021 tttgaaatct tttctaaaaa caaacaggat acaacattac tccttacagg tgatggtgta
     4081 aagatgcagg agttaaagaa tattgtgaag ataaaaaaat tagaaaatca ggttgtcttt
     4141 acaggtttta aaaaaaatgt ttttgcatgg tttaacaaaa tgaatgtatg tgtattccca
     4201 ttcgaaaacg aggcatttgg tatcgtagca attgaagctt taatgctagg taagacagtg
     4261 atagtttttt cagatggagg aggaattaca gaagtcatca agccgttaaa tagacttaat
     4321 attgttgaaa gtactgcaca tcttgttgac aggcttaatt tctattatga caataggaat
     4381 gttttagctg atgaacaaaa caacaaccgt caatatgcat cacgttttaa tatttcttct
     4441 aatgagaaag cttattttga tgtttacaaa gaagtattga ataacacttt agaatgagat
     4501 tgttttacta aaaccatttt tcaagagacg tattatacaa acctcctcag gaaggttaca
     4561 cacttttggg aattgtggct atccattgaa gatttcatct aatttattat tcaagaatac
     4621 aattatagtt gcataactat ccaagtcaat tggcctatta gcttccattg tataaatgtc
     4681 atttgtaaag agcgaaatga aatcagatag taatgaagca agacttggtt tatcagatac
     4741 tttaatcttg ttttttatgt aagtcatatt tagcctccag taattctatc ttttttaaca
     4801 aattaacatt ttgaattgtc agttcctgca cagctttaac caatggtaca acaaaatctg
     4861 catagctcaa tgtataggta tcattcgtat tttccggttt atgaattcca ctaaaattat
     4921 accctgaatt cttagcagcc tcttctactt cttgtgcaat gaagccggaa taaataattt
     4981 tttctttttg ttgtattgct gcatcatcat taaatgaatg ttcatcggtt ccaataaagc
     5041 tacaataaga ttttatatca taattatacg atacggggtt tagccgattt ataaaattta
     5101 gtcccggaat attatttttc acattggttt taaatcttcc atcagaaata atactccagt
     5161 taactgcgcc tccaatagct gttactgtag catttccgat tttgacttgt tgacttgctg
     5221 tggctattgc tccattacca atagctgtag aattgctaaa gcttgctgca tttgcatcag
     5281 cctggtaacc tacaaaagta cagttggagt atgatgtata gctatcgcct gccccggttc
     5341 ctatggcagt attattacct gtgtttccgc ttgcgttata tgcagatagt gtattatatc
     5401 caacacctac gttgttatga cttgaaatat tctgtgcaag cgaattactt ccaatggcaa
     5461 tattaaaatt acctgacaga ttatttgtaa gtgttaaagc tccaacggaa ctattatcct
     5521 taccaagagt attagcagat tgtgctccag aacctactgc agtattatta ttgccgttat
     5581 tattttgtaa agctaaactt cctatggcgg tattagcatt tcctacaata tttagtttta
     5641 atgaaaaatc acctaatgca gtgtttgaat tgccaatggt gtttgtataa agactttcat
     5701 aaccaacagc tgtattagat gttccaccat tattacttcg taatgcccgt gaaccaactg
     5761 caacaatacc tgaagcttta ttaaaatata aggtactgtc gccaattgca actactctgt
     5821 tatttgaaag tccgtctgca gcacatttgt agcccaatac agtattgtaa ctacctgttg
     5881 aattattttg caatgcaaat gctcccattc caatattata acttccggtg ttgttttcat
     5941 aaagagacaa gctgccgaat gctatattgt aggtacctga agtgttttgt cttaatgcag
     6001 tatgtccgaa tgcgcaatta tcagatcctg tattattaaa caaagtttcg gctccaaaag
     6061 cattattact tatcccggga ttagcagctt gcctcattgc taaatagcca aaagcattgt
     6121 tccaaaaacc accttcaata ttttgtaatg caccggctcc aacagctgtg ctatgcacac
     6181 atgaaatgca ttgctgcata gtttcacgac caatggctgt atttgaaaaa gtagcaactg
     6241 cgttttgtaa agattttgcg cctactgctg tgtttaatgt tccaattgta tttgcctcac
     6301 cagctttagc accaataaat gtattgtcaa aacctgtaat ggtattattg aagcctgcaa
     6361 gcttgcctaa aaacacattt ctgccaagaa aggttccttc ttgttgatca ataaacccag
     6421 cctttgtatt gttgacctta attaataatg gctgattatc agtagtacct aaaaaattgg
     6481 ttcctgacgc tgttcctgca tctccagtca aactccaacc aatattacta cctacagcaa
     6541 tccacgcaat tccattgaaa aagtaaaact tattcaggtc actatcatac actaataagc
     6601 ctgtagccgg tgctgcaatc gttatacgca atgtcgttgt catgcgtggt attaaaattc
     6661 ctttgtcagt cgaaaccata tcaagcaatg cacttgcatc gggtgtaggg ttgttaatac
     6721 ctacattttg tgcttgtatt ctaatgtgta ttagaaataa aacaaaagag aaaaagagag
     6781 aatatttgtt tgtcattgca attacaaatt ataactactg tacaaactta catctgttta
     6841 aaaacgaagt aaataccatt taagtggtag gtataaaaaa atattcagta ctttaagttg
     6901 ttgcttgcta ttaagaatgt aaggttacga ttccggatgt ttgccataaa ctttattgat
     6961 tgctgctgta cgtgagtcaa cctgtagctt ttcataaata ttataaacat gtttacgcac
     7021 cgtttcaaca gcgatttctt ttttggctgc aatctcttta tacatcaaac cctgtgctaa
     7081 caaatccaag acttcttttt cgcgtgaggt taaaatctca ttatttatta cagttgtttg
     7141 tttgtgcgaa aatgaatcta ccactaaacg tgcaatactt gcactcatag gtgaaccacc
     7201 ggatgcaatt tcacgaactg cagaaactaa cttatcacct gtattgttct ttagcacata
     7261 gcccgttgct ccattacata aggcttgaaa aatatattga gggttttcaa aaactgtgct
     7321 aatcagaaat tgtactgatg gttttttagg cttagcttca gctacacatt ctataccggt
     7381 tttgccgggc atatttatat ccatcacaac aacattacag tcaaagtttt cccatgattt
     7441 taagaaattt tctccattag aaaatgtttt tataacttcc atatcttttt cagaaccgag
     7501 aattgcctct gctaactctc gtatttcttt atcatcctca acaagaatta ttttaatttt
     7561 tttcatatac acattatagt attactaatg aaatcaaatc tgtttccctt ctgcaactat
     7621 tttacatcca tgtcctggag ttgaaataat agtaagttta ttttgatgct ggaacattcg
     7681 ttgttgcata ttaatcaatc cgtttccatt tctaattatt ttattaaaat cagttacgtc
     7741 aaaaccattg ccatcatctg ctacaataag tttaaaatta cttttgttca atgtgaaagt
     7801 tacagttata tttttagcat tggcatattt caatgaattg ttaattgcct ctttcattac
     7861 taaaaaaatg tttcttttaa tttcaggatg aatggaagaa gatatttcag tatcaggaaa
     7921 atgaagtgag taattcactc cggaatcttc caatagattg tttgctgtag atttcatata
     7981 gtttattagg ctggcaatat tatcatggcc aggattaata gcccatacta tttcacttaa
     8041 tgacgaactt acaccttttg aataggttgt gatttttgat aacgcttctg ctacttcttc
     8101 ctgatttgat tttttcttgg cgacatcgct caacaatgta atttttgtta gtccactacc
     8161 tatttcatca tgtatatcgc gggcaattcg cacccgaatg ctttgtgctt cgcgctgctt
     8221 ctcggtttca accaaattat gttgtgtttc ttttagctcg cggtatgctt tttcaatggt
     8281 tttgtttttt tctgcaagta ttaagctgct tcgatgtctt tcccggtatc gctgaaaaac
     8341 aaatccggca aaaaccaaaa gtactgaagc aattgcaatg agaaaataat gttgttgttt
     8401 ttgccttttt atttcaatag cagcaatagt tctttccttt tcaaattcaa aattcatttg
     8461 tgcctgtgta agactacgta ctttttctgt attcaaatct tcactatatt catcaaatgc
     8521 tgaatccata acattgtacg ccacagctat ttttctatct gctgctagtg ccaaggcaag
     8581 gacacggttt atttggctgt tatcattcaa ctcatctgtt ttatctgcaa ttgcacgtgc
     8641 tttacgtaac atttttatag cgtttgcaac ttttccttct gacaatttaa cctgagcact
     8701 caaggtatta aaaaccattg tctgctcagg attttccaat tcttgtatta cgggttctat
     8761 ttctgccaaa tactttttac acgaatctgt ttgcctgatc gaatgaaaaa aatcaatcat
     8821 gtcaaaagta gcactggtaa gtaacatagg gttatttgct tttttaaaaa gaagatatgc
     8881 ttgttttcta taatacaatg cactgtcatt atttattttt tcctcaatat aataattgct
     8941 tgataaggca taagccgaac ctaatattga aggaaaactg tcgcctttaa gtatttggac
     9001 tgcttgcttt gcataatcaa aagatttctc taagtcttct aattctctat gacaagttgc
     9061 tatgtaaaca taagcagtag caatgtcttt aatactattt atgttttgag cacacactaa
     9121 aaattgttga aaatacttta attgattagt gtaatcacct ttgtcaaaat atgatatccc
     9181 tgcctgacga ttaaatacca tctcaaaatt acttagtgat tctaacatgg tttcttctgt
     9241 agctgctgct ttttgttttt taacaaacat caaccctttt aatgcataat ttctcattga
     9301 gtctgattct ccaacttcga aaaacagttt gtttagttgt tcaatatttt taagataaat
     9361 tgtatctgcc aattcaaatt tctttgcagg ctggaaagtg ttaacagctt ttgaaaggct
     9421 atcataacta ttagattgag catttgagat tccaaataaa aaaacaaaag aaatcacaaa
     9481 tgatagagca aaactgtagt gtgttttaat cattgaatta actaacttta atataatttg
     9541 ctttgtgcaa ttatcttgca accctcgttt tcagaagtaa caatttgtaa tgaacagttg
     9601 tgttgcatca tcctgttccg catattaacg agaccattac ctttaatacc actttcctct
     9661 aataaatcca tattaaatcc gataccatcg tcagctatct ccatattaaa ctggttgtct
     9721 tttataagaa aaagtatagt tatattttta gcattggcat atttcaatga gttgttaata
     9781 gcctctttca ttactaaaaa aatgttcttt taatttcagg atgtatggaa gaagatatta
     9841 cagtatcagg aaaatgaagt gagtaattca ctccggaatc ttccaataga ttgtttgatg
     9901 tagatttcat atagtttatt aagaatgctt ttttatataa catcaatgct gtagaaaaat
     9961 cttgtatgcg aaaaaaattc tgtgctttta acctattaag cttagctaaa ttttcgggaa
    10021 agtattcgtt cggttgatgc ataacactat ctttaattga aattgatgta tcaaacattt
    10081 ttaactggta atacattttg caataactta atatggcctg ctgcctttta actggtttgg
    10141 ccattcttaa atacatttgt tttttgagga aataaataca ttcttcatct tgattttctt
    10201 taacataagt attagtaatg gagccaaaaa agcactataa attgaatagg gttttgtttc
    10261 gtttattttt aatattgaat cagtaaaatt aaaagcttgg gcaattgaag acacaattgg
    10321 aaaaactaaa tagatgaaga tgctaaaaaa taaaactaat tttttatcca tataccatgt
    10381 ccattgtttt caaattaaac aaaatacgtt gttcagttaa aactgattta caaattccgg
    10441 ttatatcctt aatcatttcc catgttgcaa tgttagcaaa tatagcattg gatgcagcca
    10501 tagatggggc catgtgtttg ttgttttgaa tttttattaa ttcgttttgt gaatggatgt
    10561 cttttgccca actttgaaaa atttcttcat aagtcttagt attgtatccc agcaaagggc
    10621 ctattaaccc ttgatatgcc atgtacccgc attttagata cttaaactga tattgtttgc
    10681 aaagtgctga tgcccataaa accaagtcaa ttggtttatc ggcagaaaga acaatgtagt
    10741 caactttgtt acattcattt aacataactt caaactcatt ttgtgaatgt atttcttttt
    10801 gtaacaaatt tattttacat tgtaaattca gttctttcag acgatttgca gcagcatgaa
    10861 ctttactttc accaatattg ttttcagaaa aaaggatctg tcggttaagg ttggttactt
    10921 ctacatcatc agaat
//
LOCUS       DAOOOL010000082        10907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_554_length_10907_cov_145.659141, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000082 DAOOOL010000000
VERSION     DAOOOL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_10907_cov_145.659141"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="PKU82_07770"
     CDS             complement(<1..1363)
                     /locus_tag="PKU82_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOZ82807.1"
                     /translation="MKFPSINFSVYGKRTYLVLVSVLIGALTTLCAALLKKGVMLMEH
                     IAEHLVFDSGYHQLFLILPAIGILLTVTFWRLMKIKNERRGMTAVIYNISKQSSVVAR
                     HKLFSQMVSSIFTVGFGGSAGLEAPIASTGSAIGSNIASKLKFSTKERTLLLACGAAA
                     GISAIFNAPIAGVVFALELLLIDMPVPVVIPLLISSATAAFLSRYFNLGQPFVLITDT
                     WNSSHILYYLALAALAAIISIYCIKIYFFISSLFSKITNVFVKALIGGTLLGLLIFLF
                     PPLYGEGYDSVQLLLHNQSAEIFKNITFFEANALWLPILAALLMFFLKVVATSFTVFG
                     GGNGGMFGSSLFTGAMLGFTFSRVVNIMGWAELNEINFTVIGMASMLSGVLQTPLTAI
                     FLIAEITGGYALFVPLMITTSITYLATRYVEPHSVYTKQLALKGGLINLSKDKQVISK
                     MKLR"
     gene            1624..2787
                     /locus_tag="PKU82_07775"
     CDS             1624..2787
                     /locus_tag="PKU82_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HOZ82808.1"
                     /translation="MSPNQALIKSKLPKTGNSIFSVMSALANECNAINLSQGFPDFAI
                     SPELISLVNKYMKKGMNQYAPMPGIMPLREEIAAKIEARFSAKYNPETEITITAGGTQ
                     AIYTAIASVVREGDEVIIFDPAYDSYAPAVVLAGGEPVHAELKMPDFHIDWNEVKKLM
                     NQRTRMIMINTPHNPTGAILSVKDFQMLEKITAKTDIFVLSDEVYEHVIFDGYEHQSV
                     ARFPKLAERSFIVYSFGKTFHTTGWKMGYCIAPAKLSAEFRKAHQFMVFSANTPIQYA
                     YAEFMKDAKNYSGIEKFYQEKRDNFLKLIQGTAFKPLACSGSYFQLLDYSKLSNEKDM
                     DYAVKLTKDYGVASIPVSAFYRNGTDHKLLRFCFAKSEETLEKAAERLHKVEK"
     gene            2842..3624
                     /locus_tag="PKU82_07780"
     CDS             2842..3624
                     /locus_tag="PKU82_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOZ82809.1"
                     /translation="MSTLRVTTVQTKLHWESQAKNIKHFTTLLKSIKAGSTDLIVLPE
                     MFSTGFSMNASVLAEKNDGPTMQWMQKMAETKNAVVCGSLIVEEGGRYYNRLVWMTPG
                     GAHAHYDKRHLFTMAGEQKFYHPGNKKLILLYKGWRLSVFICYDLRFPVWMRNQNEYD
                     CAIVVANWPEKRSFAWKQLLTARAIENQSFVVGVNRIGKDGNDITYSGDTVVLDPFGK
                     KISKTKSATENIETVKLDFDVLENARHIFPVGNDADSFTIKL"
     gene            3624..4034
                     /gene="arfB"
                     /locus_tag="PKU82_07785"
     CDS             3624..4034
                     /gene="arfB"
                     /locus_tag="PKU82_07785"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOZ82810.1"
                     /translation="MGKIDFKCLFSEVILTYSRSSGKGGQNVNKVSTKAELAFDIENS
                     VCLDEEQKKVITEKLCRRINEDGFLKLQSSEARSQLGNKELVLKKFQMLIEKAMHRPA
                     KRISTKIPAQVKEERLTQKRRTAEIKKLRQKNVD"
     gene            complement(4752..5381)
                     /locus_tag="PKU82_07790"
     CDS             complement(4752..5381)
                     /locus_tag="PKU82_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOZ82811.1"
                     /translation="MPSLILTSDDFGLSKIFNQRMIELLQSNFLSSISIMINQVTSDQ
                     QNQIAEIKKMYAKNNFSLGLHLEVSEADDEATYQSQWNMFEQLLSIPPDYIDVHKGHF
                     QNATFNSIAEFCLQKRVRFRKYKETTVNVSSPESSLTATFKELGAIKEWIANFKTNST
                     YELVFHIGSFDPDSKSSLNKDRELDIEKLKHVHEFISDKNISITNYKSL"
     gene            complement(5402..6481)
                     /locus_tag="PKU82_07795"
     CDS             complement(5402..6481)
                     /locus_tag="PKU82_07795"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82812.1"
                     /translation="MSENKTNKHSYLSALIQLGKFAWGIFIGISILVTVATMVTDFKM
                     TKEHPVLTGLAVLAVTIFISLILLVIKMINEGVEQKSPLPLSTELVNIINTLYEKEKY
                     LDVVRLGSALSRYLWLKGKYHERIEIGERVEDAAGKESRIEEQVSSLIDDIGWTYNII
                     GQPDKAVENITSGIDKALTNELFYYAAKGERHLSGIEKHKGHQAECLSHLQKAKEHSE
                     KILDLTSKNEMEASLFLAEAKYLFELGKLNEAEALAEKAIAVFKNDPDRIVKIHSLLG
                     NIYLKQNKLQKAKDEFNKGYNATKSTREDEVAKNLKGLADIALLERDIKSGLKYLNEA
                     KEIYLSIHKNKEATDLDNIIRSIRI"
     gene            complement(6485..7153)
                     /locus_tag="PKU82_07800"
     CDS             complement(6485..7153)
                     /locus_tag="PKU82_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ82813.1"
                     /translation="MHIVFIRHTESEKNVNNQFSSYKDKEPLTEIGMSQSENLGNDLL
                     EFKERKNLTCKNVYAASSTRAIETAKIIASKLNATVSVEDALRSTKPGALSGKSEEEG
                     LKTNPDFLEQLYLFRNGLFNAYDFTVAEGKEPKKDFEKRVWGRLSEIISDKSESLKII
                     SAHRSSITYMLLEFAKRYYKYPQNMSGHVLLDLGKISLVETVAENKWKITRVNFDSSE
                     LKTL"
     gene            complement(7154..8395)
                     /locus_tag="PKU82_07805"
     CDS             complement(7154..8395)
                     /locus_tag="PKU82_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron reductase"
                     /protein_id="HOZ82814.1"
                     /translation="MLLENLSIEKLPYLERYVNSGSPSGFTNRYTTSEETKPFGLSPF
                     FHPLICVAPEECFKNYGKIPAFKEFEEVTGKNWLLVHPDMKKEKVFKAKNVSLTKSKK
                     IKLVPTASGRTVELFNSSTKGYFKLHYTHVIGRITRELDNKIGVAGPEISKHLVSLID
                     QNKLDSRLTLMRETGARIFQGHVNEKPVEWGMVWREKDAYKIDYNEVKYVIPVFSLFG
                     KDILAKRDKPLLQQIINLIGANPTDYVLKQLIFPMIELYFNLIKNSGIQPEWHAQNLL
                     IGFTKDFAPVKFIMRDLESMDKDLTFMEEHNIKAKFESYPFKCIFREQWNYQRKHSFM
                     YDYKFGEYIIRPLLDFLKKKYKVDTLPLHQKIKLHSKKFINEFSKDFFPEYWVVFRKI
                     LINQKTDKRPYVHRKNPKFRY"
     gene            complement(8395..9054)
                     /locus_tag="PKU82_07810"
     CDS             complement(8395..9054)
                     /locus_tag="PKU82_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ82815.1"
                     /translation="MAEEYLDIYNSKGVHLGKELRKVVHQKGMWHKTFHCWVIFRNEA
                     GEDCILMQKRAAKKESAPNKLDSAVAGHYTAGEDISGGIREFKEETGIDIDSKDLIPL
                     GIRVTVSDYKPNSINKEFQEVYFIKKSIPLESFKLPVDEVAGMVELPVKKCIALLDKQ
                     IDHFFARGIFNDDASSPNIAIIIERKITIDDFIWFIDNFHFKIMLLTSKALANEKYLF
                     I"
     gene            complement(9070..10821)
                     /locus_tag="PKU82_07815"
     CDS             complement(9070..10821)
                     /locus_tag="PKU82_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ82816.1"
                     /translation="MAIFNFQNDAAIGTVFSVDTATVIIKVDEIDKLRKLHVNHLVAI
                     KSSKAGQHLIGIINRITRKASDEEKPTEEDLGLGGFLFTENILKVNLIGTLHDKHGQK
                     ENVFKRNLDTVPEIEAQCFPIDGENITKFMTTISSVDNYKVPLTIGKYSIDEHAQAFL
                     DGDKLFQRHAVIVGSTGSGKSWCVAKFLEQIAQLPMADCILFDIHGEYSGKDFKMDGV
                     QHLRVANPSDLGKKGNLANNVLMLPYWMLTYEEMLAMLLDRSDSNAPNQAMLFSKTVF
                     TEKQKFLDAIKDKIFKDHITIDSPIPYKMENVLDELQRMDEEMVAGAKVGTEKQGPFH
                     GKLTRFIQRLEAKSQDKRLGFLFQLSEDELQIDWMNTLCERLMFGSALSKEKSGVKII
                     DFSEVPSDVLPLVIGLVARVVFTVQQWTAKENQHPISLLCDEAHLYIPERTNQDAAAE
                     LGLKSFERIAKEGRKYGVSLTVISQRPAEVNRTVLSQCNNFIALRLSNAEDQAVIKKL
                     LPDNLSGLTDVLPILDIGEALVVGDASLLPTRVIIDEPKIKPESATIKFWQEWSNDKA
                     KQDIATAVTGLRKQSKA"
BASE COUNT         3317 a         2093 c         1896 g         3601 t
ORIGIN      
        1 ctcttaattt cattttgctg attacctgtt tgtctttact cagattaata agtccgcctt
       61 taagtgctaa ttgtttggta taaacagaat gtggctctac atatcgtgta gctaaataag
      121 taattgatgt ggtaatcatc aaaggaacaa aaagagcata gcctcctgta atttctgcaa
      181 tcagaaaaat tgctgtgagc ggtgtttgaa gtactccact aagcattgat gccataccaa
      241 ttactgtaaa atttatttca ttcaactctg cccaacccat aatattcacc actcgcgaaa
      301 aagtaaaacc cagcatagca cctgtaaata atgatgaccc gaacatacct ccattacctc
      361 caccaaaaac cgtaaatgat gttgctacta cttttagaaa aaacatcagc agtgctgcaa
      421 gaattggtag ccacaaagca ttagcttcaa aaaaggttat gtttttaaaa atttctgcag
      481 attgattgtg caataacaac tgaacactgt catacccttc accataaagc ggtggaaata
      541 aaaatatcag cagtccaagt aatgttccac caatgagtgc ttttacaaat acattggtga
      601 tttttgaaaa caatgagctg atgaaaaaat atatcttaat acagtaaata gaaattatag
      661 ctgccaatgc agctaaagca aggtaataga gtatgtgact tgaattccat gtgtcggtta
      721 tgagaacaaa aggctggccg agattaaaat atcgtgataa aaaagcagca gtggcacttg
      781 atatcaatag tggtatgaca acaggtacag gcatatcaat gagcaaaagc tcgagtgcaa
      841 acaccacacc tgcaataggt gcattaaaaa ttgcagaaat tcctgctgca gcaccacatg
      901 ccagcaacaa tgttctttct ttagtagaga attttaattt gcttgcaata ttgcttccaa
      961 tagctgaacc ggtagatgcg attggtgctt caagacctgc tgagccacca aaaccaacag
     1021 taaaaatact gctaaccatc tgcgaaaaca gtttgtgcct ggcaacaaca ctcgactgct
     1081 tggaaatgtt ataaattact gccgtcattc cccttcgttc atttttaatt ttcatcagtc
     1141 tccagaatgt aactgttagc aatattccta ttgccggcaa aatcaaaaac aactgatgat
     1201 agcccgaatc aaaaaccaaa tgctctgcaa tgtgctccat cagcattaca ccttttttta
     1261 ataaggctgc acataaagta gttaaagcac cgatcaatac acttacaaga accaaatatg
     1321 ttcttttacc ataaacagaa aaattaattg atggaaattt cattcaatca aaatacaaac
     1381 attaactgag caaacaaata taattgaatt caaatcaatg aagattttaa aaactgctaa
     1441 ctataaacaa aagacttcaa ttctacaacc atcaaaatct taaataaaaa tctgtgaaag
     1501 ggaatgttgt ttaaaacgat ttgctgttat actcaataca atagcattaa agcaggagct
     1561 tataaagcag atttcttttt tgttaacggc atttgattta catttgaagc atagccgctt
     1621 tttatgtcac caaatcaagc attaataaaa tcaaaacttc ctaaaaccgg aaacagcatt
     1681 tttagtgtca tgtctgcact tgccaacgag tgtaatgcca ttaatctttc tcagggcttt
     1741 cccgactttg ccatttcgcc cgaactgatt agtctggtaa ataagtatat gaaaaaaggc
     1801 atgaatcaat atgcacccat gccgggcatc atgccgctgc gcgaagaaat tgctgctaaa
     1861 atagaagccc gctttagtgc aaaatataat cctgaaacag aaattactat cactgcagga
     1921 ggaacacaag ctatttatac agccatagca tctgtagtac gcgaaggcga tgaggttatt
     1981 atttttgatc ctgcttatga ttcctatgca cctgctgttg ttcttgccgg tggcgaaccg
     2041 gttcatgcag agttaaaaat gcccgacttc catattgact ggaatgaagt gaaaaaactc
     2101 atgaaccaac gcaccagaat gattatgatt aacacaccac ataaccctac aggtgctatt
     2161 ttaagtgtta aagattttca gatgcttgaa aaaattactg caaagacaga catttttgtc
     2221 ttgagtgatg aagtgtatga gcatgttatt tttgatggtt acgaacatca gagtgtagcg
     2281 cgttttccaa aacttgccga gcgcagtttt atcgtttatt ctttcggaaa aacttttcat
     2341 accacagggt ggaaaatggg atactgcatt gccccggcaa aactttctgc cgaattcaga
     2401 aaagcacatc agtttatggt ttttagtgcc aacactccca ttcaatatgc ctatgcagaa
     2461 tttatgaagg atgctaaaaa ctattcaggt attgaaaaat tttatcagga aaaaagagac
     2521 aatttcttaa agctgattca aggcactgca tttaagccat tggcctgctc aggttcgtac
     2581 tttcaattgc tcgattacag taaactgagt aatgaaaaag atatggacta tgccgtaaaa
     2641 ctgacaaaag actatggagt ggcatcaatt ccggtttcgg ctttttacag aaacggcaca
     2701 gaccacaaac ttttgcggtt ttgttttgca aaatctgaag aaacacttga aaaagctgct
     2761 gaaaggctgc ataaggttga aaaataatac cttttctgca attataaaat ataactttgc
     2821 ctgaaccaac aacatcatac catgagcact ctaagagtaa caactgttca gacaaagctt
     2881 cactgggaaa gtcaggcaaa aaacattaaa cattttacta cactgcttaa aagtattaaa
     2941 gcaggtagca cagacctaat tgtgctgccc gaaatgttta gtacgggctt tagcatgaat
     3001 gcaagcgtac tggcagaaaa aaatgatggc cctaccatgc agtggatgca aaaaatggca
     3061 gaaacaaaaa atgctgtagt ttgcggaagt cttattgttg aagaaggtgg cagatattat
     3121 aaccgcttag tatggatgac tcccggtggc gctcatgcac attacgataa aaggcatttg
     3181 tttactatgg ctggtgaaca aaaattttat catcctggaa acaagaaatt aattctgctt
     3241 tataaaggat ggcgactctc agtttttatt tgttacgacc tgcgttttcc tgtgtggatg
     3301 cgtaatcaga acgaatatga ttgtgctatt gttgtagcta actggcctga gaaacgcagc
     3361 tttgcctgga aacaattgtt gacggcacgt gccatagaaa atcaatcgtt tgttgttggt
     3421 gttaaccgca ttggaaaaga tggaaatgac attacttatt ccggagatac agtagttctc
     3481 gatccgtttg gtaaaaaaat cagcaaaaca aaatctgcaa cagaaaatat agaaactgta
     3541 aaactcgact ttgatgtttt ggaaaatgca aggcacattt ttcctgtggg caatgatgcc
     3601 gatagtttta ccattaaact gtaatgggaa aaatagattt taaatgtctt ttttcagaag
     3661 tgatattgac ctactcgcgc agctcgggaa aaggtggaca aaatgtaaac aaggtttcta
     3721 ccaaggctga acttgccttt gatattgaaa actctgtatg tttagatgag gaacaaaaaa
     3781 aagttattac ggaaaaactt tgccgaagaa tcaacgaaga tggtttctta aaacttcaaa
     3841 gcagtgaggc acgtagccag ttaggtaata aagagctggt attgaaaaaa tttcaaatgc
     3901 tgattgaaaa agctatgcat agacctgcaa aacgaataag tacaaaaata ccggcacagg
     3961 taaaagaaga acgactgact caaaaacgca gaacagcaga aataaaaaaa ctgcgacaga
     4021 aaaatgtgga ttgaacctat caggtacaca ctatatcata ttaaaacaaa gttagtttgt
     4081 tccagttggg agagcgataa tgccgtactt cacgttcatc atattttcta cgtgtagtcg
     4141 tcatgcaaaa ttttaaacta catgtattct atattcataa aaaatgtatt aacccttcaa
     4201 tgctattttt attatttcaa agtcccctga aatgcgagag gctttgaaag taaggaaaat
     4261 gacgctcgcg cctacaagga agattatttt tacagcagtg catgtgatta ttgcggcacc
     4321 aaaggtttat tggacttact gctgcttgaa tgatagatgg gcacaagtcc gaagacttgc
     4381 gccagagggt gagttgtagc actaaacttc attcggagaa ctgaacttcc accttgcaca
     4441 aatgtgtctg cgaagcacgg aacccccgct ttcgtttaag cccttgttat gcgtatcacc
     4501 cgacttctcg caatgaaata gtgtctgtcc attgtctgca caaacctgtc ccacaaggcg
     4561 atttacattc aaggcgatgt ctgtccgagt gagcactgtg cggttgggtg atttaatttt
     4621 atttgagggg gtggagaaaa aattttcaaa ttcttcttgg gtagggcaga cacactcttt
     4681 tgcaagtttt ggtttcagcg ttggcttgta gcggcttgca aatgtgtgtg aatgctgcgt
     4741 tggcattttc tttaaagcga tttgtagttt gttattgaaa tatttttgtc tgaaataaat
     4801 tcgtgaacat gttttaactt ttcaatatca agttctctgt ctttattcaa actggattta
     4861 ctgtcggggt caaaactacc gatgtgaaaa accaattcat aagttgagtt tgttttgaag
     4921 tttgcaatcc attctttgat tgcacccaac tctttgaatg tagcagtgag agaactttca
     4981 gggctgctaa catttactgt cgtctctttg tattttctga accgaactct tttttgcaaa
     5041 caaaactctg caatagagtt gaaggttgca ttttgaaaat gccctttgtg aacatcaatg
     5101 tagtcaggtg gtattgaaag taattgttca aacatattcc attggctttg atatgtcgct
     5161 tcatcgtctg cttctgaaac ctctaaatga agtcctaaac tgaaattatt tttagcatac
     5221 atttttttta tctcggcaat ttgattttgt tggtcggaag ttacttgatt tatcatgatt
     5281 gaaattgacg aaagaaaatt tgattgcagc aattcaatca ttcgctgatt aaatattttg
     5341 cttaaaccaa aatcatctga ggttaaaatc aaacttggca tttgtcctaa atcttaattg
     5401 cttaaatcct gatactgcga atgatgttat ccaagtcagt tgcctctttg ttcttgtgaa
     5461 tagaaagata gatttctttt gcttcgttca aatattttaa tccacttttt atgtctcttt
     5521 caagcaatgc gatgtctgct aaacctttta agttttttgc aacctcatct tctcttgtgc
     5581 tttttgttgc attgtatcct ttattgaatt catctttcgc tttttgaagt ttgttctgtt
     5641 tcaaataaat atttcctaac agcgaatgaa tctttacaat cctatcgggg tcatttttaa
     5701 atactgctat tgctttttct gcaagtgctt ctgcctcatt taattttcct aattcaaaca
     5761 gatattttgc ttctgctaaa aacaaactgg cttccatttc atttttactg gtcaaatcca
     5821 aaatcttttc tgaatgttcc tttgcctttt gtaaatggct caagcattcg gcttgatgtc
     5881 ctttgtgttt ttcaattcca gataaatgtc gttcaccttt agcagcataa taaaataact
     5941 cgtttgtaag agctttatca attccactcg tgatattctc aaccgcttta tcgggttgtc
     6001 cgataatgtt gtaagtccag ccaatatcat ctatcagaga tgatacttgt tcttcaatgc
     6061 gactttcctt tcctgctgca tcttcaactc gttcaccaat ttcaattcgt tcatgatatt
     6121 ttcctttgag ccataaatat ctgcttaaag cagaaccaag ccgcactaca tctaaatact
     6181 tttccttttc gtaaagtgtg tttattatat tcaccaattc ggtagaaaga ggtaaagggg
     6241 atttttgttc aacgccttca ttaatcattt ttatcaccaa taaaatgagt gaaataaaaa
     6301 tcgttacagc aagaacagcc aagccagtga gaacaggatg ttcctttgtc attttaaagt
     6361 ccgttaccat tgtcgccact gtaaccaata ttgaaatgcc aatgaatatt ccccacgcaa
     6421 attttccaag ttggattagt gctgataaat aggaatgttt gtttgttttg ttttcgctca
     6481 tatatcaaag tgtttttagt tcggaactat caaagttcac tcttgtaatt ttccatttat
     6541 tttctgctac agtttccaca agtgatattt tacccaaatc aagtaataca tgccctgaca
     6601 tattttgagg gtatttgtaa tatctttttg caaactccaa cagcatgtat gtaatggatg
     6661 aacgatgtgc agagataatt ttcaaactct ctgacttgtc agatattatt tcactcaatc
     6721 ttccccaaac tcttttctca aaatcttttt tgggttcttt tccttccgct acagtgaagt
     6781 cgtaagcatt gaacaacccg tttctgaata gataaagttg ttctaagaaa tcgggatttg
     6841 tttttaatcc ttcctcttct gacttgcccg ataatgctcc tggctttgta gaacggagag
     6901 catcctcaac acttacagta gcatttaatt tagaagcaat aatttttgct gtttcaattg
     6961 ctcttgtaga acttgctgca taaacattct tgcaagtcaa gttttttctt tctttaaact
     7021 ccaataaatc gttgccaaga ttttcgcttt gactcatgcc tatttcagtc aacggttctt
     7081 tatccttgta agatgagaac tggttgttta cattcttttc tgattcggta tgtctgataa
     7141 aaactatgtg catttaatat ctgaatttag gattcttcct atgaacatac ggacgcttat
     7201 ctgtcttttg gttaatcaaa atctttctaa aaacaaccca atactctgga aaaaaatctt
     7261 ttgaaaattc attaatgaat ttctttgaat gaagttttat cttttgatgt aagggcaatg
     7321 tgtcaacctt gtattttttc tttagaaagt ccagaagcgg tctaatgatg tattccccaa
     7381 acttatagtc atacataaaa ctgtgcttcc tttgataatt ccactgctct ctgaaaatac
     7441 atttgaaggg ataactttca aattttgcct tgatgttatg ttcctccatg aatgttaagt
     7501 ccttgtccat tgattctaaa tctctcatga taaattttac aggagcaaaa tcttttgtaa
     7561 aacctatcaa caaattttga gcatgccact caggttgaat gcctgagttt ttaatcaagt
     7621 tgaagtaaag ctcaatcatt gggaaaatca gttgctttaa aacatagtca gttgggtttg
     7681 ctccgatgag attaatgatt tgctgcaata gcggcttatc tcttttcgcc agtatgtctt
     7741 taccaaaaag tgaaaaaaca ggaattacat atttcacttc gttgtagtca atcttgtaag
     7801 catctttctc tcgccaaacc attccccact caacaggttt ctcgtttaca tgcccctgaa
     7861 aaattcttgc accagtttca cgcatcaagg ttaatctgct gtcaagtttg ttttggtcta
     7921 tcaaggaaac aagatgcttt gaaatttcag gtcccgcaac tccaatttta ttatccaatt
     7981 ctcttgttat tcgtccgata acatgtgtgt aatgaagttt gaaataaccc ttggttgatg
     8041 aattaaaaag ctcaactgtt cttccgcttg cggtgggaac taattttatc ttctttgatt
     8101 ttgtaagtga aacatttttt gctttaaata ccttctcttt tttcatgtcg ggatgaacaa
     8161 gcaaccaatt ttttcctgta acttcttcaa actctttgaa cgctggtatc ttgccatagt
     8221 ttttaaaaca ttcttcaggt gcgacacaaa tcaaaggatg aaaaaaaggc gataacccaa
     8281 agggttttgt ttcttcggaa gtagtgtaac gatttgtaaa accacttggc gaaccactgt
     8341 ttacataccg ctccaaatag ggaagttttt ctatgctcag gttttcaagt aacattagat
     8401 aaagagatat ttttcgttcg ctaatgcttt gcttgttagc agcattattt tgaaatgaaa
     8461 attgtcaatg aaccaaataa aatcgtcaat cgtgattttt ctttctatga ttattgctat
     8521 gttcggtgat gaagcatcat cattaaaaat tccccttgca aaaaagtggt caatctgttt
     8581 gtctaaaagt gcgatgcact ttttgactgg caattcaacc atccctgcaa cttcatcaac
     8641 tgggagtttg aatgattcta aagggattga cttcttaatg aagtaaactt cttgaaattc
     8701 tttgttgatg ctgtttggtt tgtaatcaga aacagtaact ctgattccaa gaggaattaa
     8761 atctttagaa tcaatgtcaa ttcctgtttc ttctttaaac tcacgaatgc ctcccgaaat
     8821 atcttctcct gctgtgtaat gccccgctac tgctgaatca agtttgtttg gagcactttc
     8881 ctttttggca gctcgctttt gcataaggat acaatcctca cctgcttcat ttcggaaaat
     8941 cacccaacag tgaaatgttt tgtgccacat tcctttttgg tgaactactt tcctgagttc
     9001 ctttcctaag tgaactcctt ttgagttgta gatgtctaaa tattcttctg ccatgtttat
     9061 attttttaat tatgcttttg attgtttcct taatcctgtt actgccgttg caatatcttg
     9121 ttttgctttg tcattgctcc actcttgcca aaatttaatt gtcgcacttt caggtttaat
     9181 ttttggttcg tcaataatta ctcttgttgg aagcaatgaa gcatcaccta ctacaagtgc
     9241 ttctccaatg tcaagtatcg gcaaaacatc agtaagtccc gaaagattat cgggcaaaag
     9301 ttttttgatt accgcttggt cttctgcatt tgaaagtcgc aatgcaatga agttgttaca
     9361 ctgacttaaa acagttcggt ttacttccgc aggtctttga cttattacag tcaaactcac
     9421 tccgtatttc cttccttctt ttgcaattcg ttcaaaactc tttagcccta attctgctgc
     9481 tgcatcttga tttgttcttt cgggaatata caagtgtgct tcatcacaca gtaatgaaat
     9541 ggggtgctga ttttcttttg ctgtccactg ctgaactgta aatacaactc ttgcaactaa
     9601 tccgattaca agaggcaata catcagaagg aacttctgag aagtcaataa tttttactcc
     9661 tgatttttct ttgcttaaag cagaaccgaa catcaagcgt tcgcaaagtg tgttcatcca
     9721 atcaatctgc aattcatctt cagaaagttg aaacagaaat cctaaacgct tgtcttgact
     9781 ttttgcttca agccgttgaa taaatcttgt aagttttcca tgaaacggtc cttgcttttc
     9841 agttcccact ttagcaccag caaccatttc ttcatccatc ctctgtaact catccaaaac
     9901 attttccatt ttgtaaggaa tggggctatc aatcgtaatg tggtctttaa atattttatc
     9961 ttttatagca tccaaaaact tttgcttctc cgtaaaaacg gtttttgaaa aaagcattgc
    10021 ttgattcggt gcgttgctat cgcttctgtc aagaagcatt gcaagcattt cctcataagt
    10081 gagcatccag tagggcaaca tcaaaacatt atttgcaaga ttccctttct tacctaagtc
    10141 agatggattg gcaacacgca agtgttgcac accatccatt ttaaaatctt ttccgctgta
    10201 ttctccgtga atgtcaaaca aaatgcaatc agccattggt agttgtgcaa tctgttcaag
    10261 gaactttgca acacaccatg atttacctga accagtgcta ccaacaatta ccgcatgtct
    10321 ttggaaaagt ttgtctccat ctaaaaatgc ctgtgcatgt tcgtcaatag aatattttcc
    10381 aattgtcaga gggactttat aattgtcaac acttgaaatt gttgtcatga acttggttat
    10441 gttttctccg tcaataggaa aacattgtgc ttcaatttca ggaactgtgt caaggtttct
    10501 cttgaaaaca ttttctttct gtccgtgctt atcatgaagt gttccgataa gatttacttt
    10561 caaaatattt tcagtgaaaa gaaacccacc taaacccaaa tcttcttcgg ttggtttttc
    10621 ttcgtcagat gcttttcgtg tgatacgatt gataatacct atcagatgtt gtcccgcttt
    10681 tgaactcttg atagcaacca aatgattgac atgcaacttt cgcagtttat caatttcatc
    10741 aaccttgata attacagttg cagtgtctac actgaacact gttccaattg ccgcatcgtt
    10801 ttgaaaatta aaaattgcca tgtcttacga tttgataagt tttaaatact ctcccaatgt
    10861 ccagtagctg acatcaggaa caatttgttc accagcgaag ctggatg
//
LOCUS       DAOOOL010000083        10882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_557_length_10882_cov_48.090422, whole genome shotgun sequence.
ACCESSION   DAOOOL010000083 DAOOOL010000000
VERSION     DAOOOL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_10882_cov_48.090422"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKU82_07820"
     CDS             <1..647
                     /locus_tag="PKU82_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ82817.1"
                     /translation="AVVGTTGSGKTTIANLMSRLYDPTQGEILVNDIPLKNINLEWWR
                     RQVGFITQDVLLFSDTIANNIAFGIKEYNSETLTEAAKTAMVYEEIVHFKDGFNTMLG
                     ERGVNLSGGQKQRISIARAIMKQPQFLILDDCLSALDTGTEEQLLQNLLPYFQHRITL
                     ILGHRISSVKHADEIIVLHQGKILERGTHLSLLQQNGFYSKLVTEQIKKQEVKS"
     gene            722..1570
                     /gene="prmA"
                     /locus_tag="PKU82_07825"
     CDS             722..1570
                     /gene="prmA"
                     /locus_tag="PKU82_07825"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOZ82818.1"
                     /translation="MNTYIAIHCKVEPVQPFSEILIAQLAEIGFEMFEEKEDGFDGYI
                     RKENFNESLLETIEFLQPNDFCSVHWQLEEIESQNWNEEWEKNYPPIRVKNIYVRAPF
                     HPDATESDLEIIIQPKMAFGTGHHATTAMMMELMLEANIADKRVLDMGCGSGILAILA
                     SKLGCSSATAIDNDPNCVLNSIENLENNKIRNVEVLQGDASSLIDLKFEVILANINRN
                     ILLDDIASYNNALPDGGQLLVSGFLTEDVELITQSFGKYGLKLSSMKQNQNWVAIHFF
                     KTHSAK"
     gene            1567..2829
                     /locus_tag="PKU82_07830"
     CDS             1567..2829
                     /locus_tag="PKU82_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ82819.1"
                     /translation="MSEHKETKNPLNIIVIVAALGYFVDIYDLILFGIVRTPSLNGLG
                     ITDPDLVTAYGLHLLSYQMIGMLIGGLLWGIMGDKKGRVSVLFGSIIMYSIANIANAY
                     VKEIADYTSSSIINTYTVLRFIAGVGLAGELGAGITLVNETMTKEHRGYGTMIVVSFG
                     VLGAVFANILGHFVTWQSSYIIGGVMGLVLLFLRIGVYESGMFKNLQVHSRERGNLMM
                     LFNNRKRFLTYLSSIAVGVPIWYMIGMLVVFSPEIAKMNGITGVNGGDAIMYFYLATC
                     IGDFLSAYWSQKLKSRKKVLALYLLLAICITPVYLYMMSHATIAVFYFICALLGFASG
                     YWAVFVTTASEQFGTNIRATVTTTVPNMVRGSLAILTLLLSYFRDSLQMSMIDAMFLV
                     GIISIAGAFLSLFGIKESYAKDLNYIES"
     gene            2830..3195
                     /locus_tag="PKU82_07835"
     CDS             2830..3195
                     /locus_tag="PKU82_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOZ82820.1"
                     /translation="MTQKKTLVLGASTNPARYSNKAILKLRYYKHPVVAIGGKPEKVE
                     DITIETGNVKFKDIDTVTMYLNAERQKSLEDYILSLKPKRIIFNPGAENESLKTKAEK
                     QGIECVEACTLVMLTIGAY"
     gene            complement(3189..3482)
                     /locus_tag="PKU82_07840"
     CDS             complement(3189..3482)
                     /locus_tag="PKU82_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82821.1"
                     /translation="MNIYNLVLFSSCLFLTQSALGQDQARPIKTGGRPAEVREINNTP
                     VIMIHKSNDVHDKGNSNMQQRKSPRCIKKVGACTEGGDRKKELKEPVRINENQ"
     gene            complement(3530..4861)
                     /locus_tag="PKU82_07845"
     CDS             complement(3530..4861)
                     /locus_tag="PKU82_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ82822.1"
                     /translation="MKLQLKLAIYNALSKALVVLAFGALMPFIVEKVVYNHIDQRLKA
                     RTSKVLRIIEKGGLNDIARESDCTFESYNILKEEFILIEPLSGMKDVKPTVITNEEWE
                     IDGEKLNHRIIRQSFIYDNQIYSLQIGEGIDSLNKLKNTISKVALWATILTIIVSIFV
                     DVGYARLLLRPLNEIVERKLKGTKDPVHFNDVPVETSTLEFQQLDISITELMQRIKAA
                     FMTEKEFISNVSHELQTPISILKTRFENMLMDEKIDDDVADKLDDSIRKLNRMSKIIK
                     SLLMISKIDNEQFLKNETVNIKALLQEIAEDFQEHLSTKNIRLYMQHLDEFEIQNANR
                     TLLYTLFSNIITNAIKYNVENGQIDISATKQSNVFVVAIRDTGVGIAKENLTHIFDRF
                     KKFNKSTEPSFGLGLAIVKTISDFHRLNLHVESEANKGTTFRVEFPIEPFT"
     gene            complement(4884..5561)
                     /locus_tag="PKU82_07850"
     CDS             complement(4884..5561)
                     /locus_tag="PKU82_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82823.1"
                     /translation="MNVLIVEDERSLSHEMEIYLTKQGLHCDVAFTGAKALEMMWVNS
                     YDFVLLDIGLPDTNGFEILKEAKKNAIDSAFIVLTARSETDDKILGLDLGADDYLAKP
                     FSLPELHARMQAILRRKHGLKVQIIDINGFMMDYQNRTLTFEDAPVVLTKKEFDLLHY
                     LALHKGRVLSRSQLTEHIWGDVMDDDYDSNYIDVHVKNLRKKLAAHAPIEWFETVRGI
                     GYRLNAQ"
     gene            complement(5644..6822)
                     /locus_tag="PKU82_07855"
     CDS             complement(5644..6822)
                     /locus_tag="PKU82_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOZ82824.1"
                     /translation="MINHRQLFLQHVAQTSQAPLALHIVRADGCFLYDDKGKEYIDLI
                     AGISVCNLGHNNSAINEAVKKQIEQYSHIMVYGELIESPQTLFASLLASHLPPNLNCT
                     YFVNSGTEAIEGAMKLAKRTTGRNEIVALTNSYHGSTQGSLSLMSNEYFSSAFRPLIP
                     GVKFIDPTKPDFDVLNENTSCIIMELVKAEEGCKPLTYDFVNNVVRICREKNILLVVD
                     ECQTGLGRTGKSFAFENYDIIPDILVLGKALGGGMPLGAFISSKKTMDKLTHNPVLGH
                     ITTFGGHPVCCAAGMAAFAQLTEQKLYDNVEVKEMLFRKLLKHPKIKSVSGKGLLLAL
                     EFESEKVCLDTIRKCIDNGLFTDWFLFAPNCLRIAPPLTISNQLIERSCEILLNALND
                     "
     gene            complement(6815..7621)
                     /locus_tag="PKU82_07860"
     CDS             complement(6815..7621)
                     /locus_tag="PKU82_07860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOZ82825.1"
                     /translation="MKDLEQLTNKTCEAVVKAAQFIQQESKRFDKSVVEYKGLHDLVS
                     YVDKTAEKILVEEFRSILPSAGFITEESTVSKSNNELQWIIDPLDGTTNFIHGLPCYC
                     VSIGLMENNKLQMGIVHEINFNETFYCWKNGGAWLNGKQISVSPTNKLQHALLATGFP
                     YTNYSRMEEYMKVFDFCMRNTRGLRRLGSAAADLAYTACGRLDGFFEYGLNAWDVAGG
                     ALLVEEAGGVVADFSGEKDFIFGQEIVACNKAVFDEFMEVIYTSFRNKND"
     gene            complement(7636..10002)
                     /locus_tag="PKU82_07865"
     CDS             complement(7636..10002)
                     /locus_tag="PKU82_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOZ82826.1"
                     /translation="MDFQATNKKNYPSEIVQIKRTGNVFLIEATNCSLEVQVYSSRII
                     RFRYGIDYLSRDFSYAKAPDFNFTETPVEQIEDDKQVVIKTSEIICRIERDTLSISLF
                     TIDGQLINCDDGGFHWEPNPGFGGHYVYCTKQIQQHENFFGLGDKATSQNLRGKRFLN
                     WGTDTYGFAKEQDPLYRNIPFYFGLHHNISYGIFFDNSFQTFFDFGKESNTHASFWAE
                     GGQMCYYFIYAPTLMQVAEEYCKITGLPELPPKWTLGYHQSKWSYYPETVVRELAGHF
                     RSKAIPCDVIHLDIDYMKGFRCFTWDYERFPDPKRMIAELREQGFKIIVIIDPGIKKD
                     KDYWVFQQALEKDYFCRRQDGDYMEGKVWPGDCYFPDFTHPEVRKWWSGLYKELLEQG
                     VSGVWNDMNEPAVFEIATFPDDVRHNYDGLNVSHRMGHNVYGMQMARATYEGLKQFVP
                     EKRPFVLTRSGFSGVQRYAAVWTGDNVASWEHIWVANMQCQRLSISGISFCGTDIGGF
                     IGEPDGELFVRYIQMAIFHPFFRGHSSSDQGDKEPWAFGAAYEALIRKSIELRYQLIP
                     YIYTTFWQYVNKGTPMIRPLAFLAQDDVETHMRMDEFGFGDQMLICPINVSASSGRAV
                     YLPKGNWYYYWNDKQFKGGHEYNVEAPLDRFPLFVKEGAVIPHQPVMQNLDGPTHQIT
                     LHVYFSSSRNTSYFYEDDGENYSYKDGNFSVKKFTVFGNRRQMRIICEKFGSFKNQYT
                     ECRMVIHGLPFKPNHFRINDMEVALPKRLRDKTITISVPADFSRIQIS"
     gene            complement(10149..10868)
                     /gene="rlmB"
                     /locus_tag="PKU82_07870"
     CDS             complement(10149..10868)
                     /gene="rlmB"
                     /locus_tag="PKU82_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOZ82827.1"
                     /translation="MYGTRAVMEAVNAGKDIEKIFIQKGINNSLVNELRQLLTKNNIP
                     FQVVPDFKLNKLTKGNHQGVVCILSSIVYHKTEDIIPLIFERGEVPLFLMIDRVTDVR
                     NIGAMARTAACAGVHAIIIPDQGSAQLNADAIKTSAGALHNIPVCREKNLKLTIEYLK
                     GSGIKIVAATEKASQPYFTSDMKMPCCIIMGSEDSGISPEYLKRADEKVLIPMNNVVQ
                     SLNVSVAAGIILFEAFRQRNN"
BASE COUNT         3358 a         2130 c         1893 g         3501 t
ORIGIN      
        1 tagctgtagt tggaacaaca ggctccggta aaactactat tgcaaatcta atgtcgaggc
       61 tctacgatcc aacacaagga gaaattttgg ttaacgatat tccacttaaa aatattaacc
      121 ttgaatggtg gcgcaggcag gttggcttca tcacgcagga tgtacttttg ttttctgata
      181 ccattgccaa caatattgca tttggaataa aggagtacaa ttcagaaacg ttgacagagg
      241 ctgcaaaaac agctatggtt tatgaggaaa tagttcattt taaagatggc tttaacacca
      301 tgctcggtga acgtggtgtt aatcttagtg gaggacagaa acaacgcata tccattgcac
      361 gtgccattat gaaacagcca cagttcttga ttcttgatga ttgtctttct gcactcgaca
      421 ccggcacaga agaacaattg ttgcaaaacc tgttacccta tttccaacat cgcataacat
      481 taattttagg tcatcgcatt tcatctgtta aacatgccga tgaaataatt gtattgcatc
      541 agggcaaaat acttgagcgt ggcacacatc tgtcattgct gcaacaaaat ggcttttatt
      601 caaaattggt taccgagcaa attaaaaaac aggaagtcaa atcctgattt tctgtaaaca
      661 tatcaactgc caataaacta tgtttgcata aataaagttg attgatttta tttgccttcg
      721 gatgaataca tatatagcca tacattgtaa agtagaacct gttcagccat tcagtgaaat
      781 actgatagcc caactcgctg aaatcggttt tgaaatgttt gaagaaaaag aagatggctt
      841 tgatggatat atcaggaaag aaaattttaa tgagtcctta ttagaaacaa tagaattttt
      901 gcagccaaac gatttttgct ctgtgcactg gcagttagaa gaaatagaaa gtcaaaactg
      961 gaatgaagaa tgggaaaaaa attatccgcc aataagagtt aaaaatattt atgtgcgagc
     1021 tccatttcat cctgatgcaa cagagagtga tcttgaaatt atcattcagc ctaaaatggc
     1081 atttggcaca gggcatcatg ccacaactgc tatgatgatg gaactgatgt tggaagctaa
     1141 tattgctgat aaacgtgttc ttgatatggg gtgtggctca ggcattctgg caatacttgc
     1201 atccaaactt ggttgtagtt ctgcaactgc cattgataac gacccaaact gtgtgcttaa
     1261 cagtattgag aatcttgaaa ataataaaat cagaaacgtt gaagtgctgc agggtgatgc
     1321 atcatcactt atagatttaa aatttgaagt tatacttgcc aatataaacc ggaatatttt
     1381 attggacgat attgcatctt acaataatgc attgcctgat ggaggtcagt tgttggtgag
     1441 tggttttctg acagaagatg ttgaacttat cacacaatca tttggaaaat atggtttgaa
     1501 actttcaagc atgaagcaaa atcagaactg ggtagccata cattttttca aaacccattc
     1561 cgctaaatga gcgaacataa ggaaactaaa aatccgttaa atatcattgt cattgtagcc
     1621 gcattaggct attttgttga catctatgat ttgattctat tcggtattgt acgtacgcca
     1681 agccttaacg gcctgggcat taccgatcct gatttagtaa cagcgtatgg cttacacctt
     1741 ttaagttatc agatgatcgg tatgctcatt ggtggattgc tttgggggat tatgggtgat
     1801 aaaaaaggtc gtgtttccgt tttattcggt tctattatca tgtactctat tgccaatatt
     1861 gctaacgctt atgtaaaaga gattgcagat tatacttctt catcaataat aaatacctat
     1921 acagttttac ggtttattgc aggtgtagga ttagcaggag aactaggagc aggaataact
     1981 ttggtaaatg aaaccatgac taaagaacat cgtggttatg ggactatgat tgtagtttca
     2041 tttggtgttt tgggagcagt ttttgcaaat attctcggac actttgtaac atggcaatca
     2101 tcatacatta ttggcggtgt aatgggattg gttttactgt ttctgagaat tggtgtttat
     2161 gaaagtggca tgtttaaaaa tcttcaggtg catagccgtg aacgcggtaa tctgatgatg
     2221 ctattcaata acagaaaaag attcttaacc tatctgagta gtattgccgt gggagtacca
     2281 atctggtaca tgattggaat gttagttgtt ttttcacctg aaattgcaaa aatgaatggc
     2341 atcacaggcg ttaatggtgg tgatgctatt atgtatttct atttagcaac ctgtattggc
     2401 gattttttaa gtgcatactg gagtcagaaa ttaaaaagca gaaaaaaagt attagcgttg
     2461 tatctcttac ttgctatttg tattacccct gtttacctct acatgatgag ccatgctact
     2521 attgcggtat tttattttat atgtgctctg ttaggatttg catcgggcta ttgggcagtt
     2581 ttcgttacaa cagcatcaga acagtttgga accaacatca gagctacagt aacaacaaca
     2641 gtacccaata tggtacgtgg gtcgttggca attttaactt tacttctgtc ctatttccgt
     2701 gatagtctgc aaatgtcaat gattgatgct atgtttttag taggtataat ttcaatagca
     2761 ggagctttct tatctttgtt tggcataaaa gaaagttatg ctaaagactt gaattatatt
     2821 gaaagttaaa tgacacaaaa gaaaacatta gtgctgggtg cttcaacaaa tcccgcacga
     2881 tattcaaaca aagccatttt aaaactgcgc tactacaaac atcccgtggt agcaataggc
     2941 ggcaagcccg aaaaagtaga agatattact attgaaacgg gaaatgtaaa atttaaagat
     3001 attgatactg ttacaatgta tcttaatgca gagcggcaaa aatcattgga agactacatt
     3061 ttatctctta agccaaaaag aataattttt aatccaggag cagaaaacga atcacttaaa
     3121 actaaagcag aaaagcaagg tattgaatgt gtggaagcat gcacattggt gatgctcacc
     3181 attggggctt actgattttc gttaatcctt acaggctctt tcagttcttt cttcctgtca
     3241 ccgccttcgg tgcaagcacc aactttttta atacaacgtg gactttttct ttgttgcatg
     3301 tttgaatttc ctttatcatg tacatcattc gatttatgaa tcatgattac aggagtgttg
     3361 ttaatctctc taacctctgc aggacgtcca ccggttttta ttgggcgagc ctgatcctga
     3421 cccaatgcac tctgagtcag aaataaacag gatgaaaata aaaccagatt gtaaatattc
     3481 attttgttgt tgtaatgtga aagcaaattt acgacattgc cggtgaaatt caagtgaaag
     3541 gttctatagg aaattcaact ctgaatgtgg tgcctttgtt tgcttccgat tctacatgaa
     3601 gatttaaacg atgaaaatca gaaatggttt taacaatagc taatcctaat ccaaaacttg
     3661 gctctgttga cttattaaat ttcttaaacc tatcgaaaat atgtgtgaga ttctcttttg
     3721 ctataccaac gcctgtatct ctgattgcaa ccacaaaaac attactctgt ttggtagcac
     3781 taatatcaat ctgtccattt tctacattat acttaatagc attagtaatg atattactaa
     3841 aaagtgtata gagcaaagtt ctatttgcat tctggatttc aaactcatcc aaatgctgca
     3901 tgtaaagtct gatattttta gtacttaaat gctcttgaaa atcttctgca atttcctgaa
     3961 gtagtgcttt tatattcacg gtttcatttt tcaaaaactg ttcattatca atttttgaaa
     4021 tcatcagcaa tgatttgatg attttactca tcctgttcag ctttctgatt gaatcatcga
     4081 gtttatcagc tacatcatca tcaatttttt catccatgag catattttca aatctagttt
     4141 tcagaataga aattggtgtc tgtaactcat gcgatacatt tgagataaac tctttctcag
     4201 tcataaatgc cgctttaata cgttgcatca actctgtaat gctaatatct aactgttgaa
     4261 actcaagcgt tgaagtctct acaggaacat cattaaagtg taccgggtct ttagtgcctt
     4321 taagttttcg ctccacaatt tcgtttaacg ggcgcaacaa aagtcgtgca tagcctacat
     4381 caacaaatat tgaaacaata atggtgagaa tggtagccca aagagccact ttactaatcg
     4441 tgtttttaag tttattgagt gagtcaatcc cctcgcctat ttgcaaactg taaatctgat
     4501 tgtcgtaaat aaatgactgc cggatgatgc ggtgattcaa cttctctcca tcaatttccc
     4561 actcttcgtt ggtaattact gtaggcttaa catctttcat tcccgatagt ggttctatca
     4621 gaataaattc ttctttaagg atattgtaac tttcaaaagt acagtcagac tcgcgtgcaa
     4681 tgtcattgag tccacctttt tcaatgatac gcaacacttt tgatgtacgc gcctttaacc
     4741 tttgatcaat atgattataa acaacctttt ctacaataaa aggcattaat gccccgaatg
     4801 ccagcaccac aagtgcctta ctcagggcat tataaatagc aagttttaac tggagcttca
     4861 tctcccgcca aaggtaatgt taatcattgg gcatttaatc tgtagccaat accgcgaact
     4921 gtttcaaacc attcgatagg tgcatgtgca gctaattttt tacgaaggtt ttttacgtgg
     4981 acatcaatat agttagaatc gtaatcgtca tccatgacat cgccccagat gtgttctgtt
     5041 aattgtgaac gagataaaac acggccttta tgcaatgcca gataatgcaa taagtcaaac
     5101 tctttcttag tcaaaacaac aggtgcatct tcgaaagtca gcgtacggtt ctgatagtcc
     5161 atcatgaatc cgttgatatc aattatttga actttaagtc catgctttct tcttaaaata
     5221 gcctgcatgc gtgcatgtag ttcaggaagt gaaaacggtt tggctaagta atcatcagca
     5281 cctaaatcca gacccagaat tttatcgtct gtttcgcttc ttgctgtcag tacaatgaat
     5341 gcactgtcaa tagcattctt cttggcttct tttaaaattt caaagccatt ggtatcgggc
     5401 aaaccaatat ccaataaaac aaaatcgtag gagtttaccc acatcatttc taatgctttg
     5461 gcaccggtaa atgcaacatc gcaatgcaag ccctgttttg tcaggtaaat ttccatttca
     5521 tgagataaac tgcgttcatc ttcgacaatt aatacattca taatttctgt gttttgaaag
     5581 gttaaaaata aaaaacttca tgtacatgaa gacaaaaata ctgccgaaat atgaagaaac
     5641 cgattaatcg ttcaatgcgt tcagcaatat ttcacatgaa cgttcaatga gttgattgct
     5701 tatcgttaaa ggaggcgcta ttctcagaca atttggtgca aataaaaacc agtcggtaaa
     5761 caagccgttg tcaatgcact ttctgatggt atccaaacaa actttttcag attcaaattc
     5821 taatgcaagt aataaccctt ttcccgaaac agatttaatt ttaggatgct ttaataattt
     5881 tcggaaaagc atttctttta cttctacatt atcataaagt ttttgttcag tcaattgagc
     5941 gaatgctgcc attcctgctg cacaacaaac cggatgcccg ccaaaagtgg ttatatgacc
     6001 cagaacaggg ttgtgcgtta acttgtccat tgttttctta gatgagataa atgcacctaa
     6061 aggcattccg ccacctaatg cttttcctaa aactaaaata tcgggaataa tatcataatt
     6121 ttcaaaggca aacgacttac ctgttctgcc caatccggtc tgacattcat caacaactaa
     6181 cagaatattt ttctctctgc aaattcgcac aacattattt acaaaatcgt atgttagtgg
     6241 cttacatcct tcttcggcct tcaccaattc catgatgata cacgatgtgt tttcgttcaa
     6301 cacatcaaag tcaggttttg tcgggtcaat aaatttaaca cccggtatta aaggcctgaa
     6361 cgctgatgaa aaatattcat tactcataag actcaatgag ccttgtgtgc tgccatgata
     6421 ggaattagtt aatgcaacaa tttcattcct tcctgtagtt cttttggcta acttcatagc
     6481 cccctctatg gcttcggttc cggaatttac gaagtaggtg caatttaaat ttggtggtaa
     6541 atgcgatgca agcaagcttg caaacaaggt ctgcgggctt tctatcaatt cgccataaac
     6601 cattatgtga ctatattgct ctatttgttt tttgacagcc tcattgatag cagaattatt
     6661 atgacctaag ttacatacag aaataccggc aattaaatca atgtattctt ttcctttatc
     6721 atcatataaa aaacaaccat cggcacgcac aatatgcaac gccagtggcg cctgacttgt
     6781 ttgcgcaacg tgttgcaaaa aaagttgccg gtgattaatc atttttattt ctgaatgaag
     6841 tgtaaataac ttccataaac tcatcaaaaa cagccttatt gcaggctact atttcctgtc
     6901 caaaaataaa atctttttct cctgaaaaat ctgcaaccac acctcctgct tcttcaacta
     6961 ataaagcgcc tcctgcaaca tcccatgcat tgagaccata ttcgaaaaat ccatcaaggc
     7021 gaccacatgc agtataagcc aaatcggcag cagcagaacc aagtcttctt agacctcttg
     7081 tattccgcat acaaaagtca aatactttca tgtactcttc cattcttgaa taattggtgt
     7141 atggaaatcc tgttgccaac aatgcatgtt gcaacttatt tgttggtgat actgaaattt
     7201 gttttccatt gagccatgca ccaccattct tccagcaata aaaggtttca ttaaaattta
     7261 tctcatgcac aatgcccatt tgcagtttat tgttttccat caatccaata ctgacacaat
     7321 aacaaggtag tccgtggata aaattggttg ttccatcaag cggatcaatt atccattgta
     7381 attcattgtt acttttagat accgttgact cttccgtaat aaatcctgct gaaggtaaaa
     7441 tacttctaaa ctcttctact aaaatttttt ctgctgtttt atccacataa ctcactaaat
     7501 catgtagtcc tttatactcc acaactgatt tgtcaaatcg tttgctttcc tgctgaataa
     7561 actgtgctgc cttaactaca gcctcacagg ttttgttggt taactgttct aaatctttca
     7621 ttgaccttaa aaaaattaag atatctgtat gcgactgaaa tctgcaggca cactaatggt
     7681 aatagtttta tcgcgcagtc gttttggcaa agcaacttcc atatcgttta ttctaaaatg
     7741 atttggtttg aaaggcaagc catgaataac cattctacat tctgtatatt gatttttgaa
     7801 tgaaccaaat ttctcacaaa ttattctcat ttgtcttctg ttgccaaaca cagtaaattt
     7861 tttcactgag aaattaccat ctttatatga ataattttct ccatcatcct cataaaaata
     7921 acttgtattt ctacttgatg aaaagtaaac atgcaaagta atctgatgtg tagggccatc
     7981 cagattttgc attacaggct gatgcggaat aacggcacct tccttcacaa agagtggaaa
     8041 acggtcaagt ggtgcctcta cattatattc atgtcctcct ttaaattgtt tatcattcca
     8101 atagtaatac cagtttcctt ttggcagata aaccgctctt ccgcttgatg cacttacatt
     8161 gattggacaa ataagcatct ggtctccaaa accaaattcg tccatgcgca tgtgagtttc
     8221 tacatcatct tgtgcaagga aagccaatgg tcttatcatg ggtgttcctt tattgacata
     8281 ctgccagaat gttgtgtata tgtaaggtat taactgatac cggagttcaa ttgattttct
     8341 gataagtgct tcatacgctg cgccaaaagc ccaaggctct ttatctccct gatcgctgct
     8401 tgaatgtcct ctgaaaaacg gatggaaaat tgccatctga atataacgga caaacaattc
     8461 gccatcaggc tcacctataa atccaccaat gtctgttcca caaaaagaaa ttccggaaat
     8521 gctcagtcgc tgacattgca tgttagcaac ccatatatgc tcccacgatg caacattatc
     8581 gcctgtccac acagcagcat aacgctgcac acctgaaaat ccggagcgtg ttaaaacaaa
     8641 aggtcttttt tcgggcacaa attgttttag tccttcatag gtggcacgtg ccatttgcat
     8701 accataaaca ttatgtccca tgcgatggct tacattcagt ccatcataat tatgtcttac
     8761 atcgtcagga aatgtagcaa tttcaaaaac cgcaggctca ttcatatcat tccatacacc
     8821 actaacaccc tgctccaaaa gttctttata caaaccactc caccactttc ttacttcagg
     8881 gtgagtgaaa tccggaaaat aacaatcgcc aggccacact ttgccttcca tataatcgcc
     8941 atcctgcctg cgacagaaat aatctttttc aagtgcctgt tgaaacaccc aataatcttt
     9001 gtctttttta attcctggat caatgattac aataatttta aaaccttgtt cacgcaattc
     9061 tgcaatcatc ctttttggat cgggaaaacg ttcatagtcc caggtaaaac atctgaaacc
     9121 tttcatgtag tcaatgtcaa gatgaataac atcacaggga atagccttac tgcgaaaatg
     9181 cccggccagt tctctaacta ctgtttcagg ataatagctc cacttacttt gatgatatcc
     9241 taaagtccat tttgggggca gttcaggaag ccctgttatt ttacaatact cttcagccac
     9301 ctgcatcaat gtaggtgcat aaataaaata gtagcacatc tgaccacctt ctgcccagaa
     9361 acttgcatga gtattgcttt ctttgccaaa atcaaaaaat gtctgaaagc tgttatcgaa
     9421 aaatataccg tatgaaatgt tgtgatgtaa tccgaaataa aaagggatat ttctgtataa
     9481 tggatcttgt tctttagcaa atccataagt gtcggtgccc cagtttaaaa atcttttacc
     9541 tcttaaattc tgagatgttg ccttatcacc aagtccgaaa aaattttcat gctgctgaat
     9601 ttgttttgta caataaacat aatgaccacc aaaacccgga ttaggctccc aatgaaatcc
     9661 gccatcgtca caatttatca attgcccatc aatagtaaag agtgaaatac tcagggtgtc
     9721 acgttctatt cggcaaataa tttcagaggt tttaatgact acctgcttat catcttcaat
     9781 ctgttcaaca ggtgtttcag tgaaattaaa atcaggtgcc tttgcataag aaaaatcacg
     9841 tgataaataa tctatgccat aacgaaaacg aattatcctt gaagaataaa cctgtacttc
     9901 taaactacaa ttggtagctt ctattaaaaa tacattacct gtccgtttta tctgaacaat
     9961 ttcagacgga taattcttct tatttgttgc ttgaaaatcc ataaaaaatt tagtgttggg
    10021 aataatttta ttccaccatc ttctcttgat gcaaataaaa caatttacca atatgctttc
    10081 ggcatatcac gctaagagtt tttaagttga aattgttgat gaaagtgaat gactaagtta
    10141 actgtttgtc agttgtttcg ctgcctgaat gcttcaaaaa ggatgatgcc ggctgcaaca
    10201 gaaacattta atgattgaac cacattgttc ataggtatta gcactttttc gtctgccctc
    10261 ttcagatatt caggtgatat accgctatcc tcactaccca ttataatgca acatggcatt
    10321 ttcatatctg atgtaaaata aggctgactg gctttttctg ttgctgccac aattttaatt
    10381 ccagagcctt tcagatactc aatggtaagt ttaagatttt tctcgcgaca cacaggaatg
    10441 ttatgcagtg cacctgcaga tgttttaata gcatcggcat tgagttgtgc tgatccttga
    10501 tcaggaatta tgattgcatg aactcctgca caggcagcag tgcgtgccat agcgccaata
    10561 ttgcgcacat ctgttacacg atcaatcatt aaaaaaagtg gcacttctcc tctctcaaaa
    10621 atcaatggaa taatatcctc tgttttatga tagactattg aggacaaaat acaaacaaca
    10681 ccttgatgat ttccttttgt aagtttgtta agtttgaaat cgggtactac ctgaaaagga
    10741 atattattct ttgtaagtaa ttgcctaagc tcattcacca aactgttgtt gatacctttt
    10801 tggataaata ttttttcaat atcctttcct gcattcacgg cttccataac agcgcgtgtg
    10861 ccgtatatta gttgttcatt ac
//
LOCUS       DAOOOL010000084        10870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_560_length_10870_cov_83.901988, whole genome shotgun sequence.
ACCESSION   DAOOOL010000084 DAOOOL010000000
VERSION     DAOOOL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_10870_cov_83.901988"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..168
                     /locus_tag="PKU82_07875"
     CDS             31..168
                     /locus_tag="PKU82_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HOZ82828.1"
                     /translation="MQKFQSAGPKGVTKLPHPFFGKMTDAEWDKLQCKHLDHHLRQFG
                     V"
     gene            165..725
                     /gene="lptC"
                     /locus_tag="PKU82_07880"
     CDS             165..725
                     /gene="lptC"
                     /locus_tag="PKU82_07880"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOZ82829.1"
                     /translation="MKFRFVPVVFIISLLMMSCENDIEQVKLVTGNVVEPIESAKGLE
                     ILYSDSGKVKVRIAAGEMNRYLQPKPITEMPKGVKIDFFDDNLKVTSTLTSDYAVRDD
                     FNKNMEAKKNVEVVNVKGEKLMTEYLKWDEKTGLISSPEFVKIITADEIIYGNGFESN
                     QDFSRYKIFNIKGIINLKKNENTENT"
     gene            703..900
                     /locus_tag="PKU82_07885"
     CDS             703..900
                     /locus_tag="PKU82_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82830.1"
                     /translation="MKILRILEISWLVIAIGSLCAGIYKLISAGLNQAVFIFAISIIA
                     FVFFMIRRKQRIAMEKQNASR"
     gene            938..2221
                     /locus_tag="PKU82_07890"
     CDS             938..2221
                     /locus_tag="PKU82_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOZ82831.1"
                     /translation="MISDFLPAILASLAFVAFYSGIEIAYLSSNKFKIELDSKQGKWS
                     GKILSYFSKSPSRFICTILVGLNISLVIYGTNMSGLLNPYLQQWLPQQLNSEIGRLII
                     ETLITTFFLLLAGEFLPKILFSINPNDTLNFFSGFIWISYILLFPLVWLILKLAHFLL
                     RNVFKVDFVEETSGFGRVDLDNFIEEISENTGGKSEHSKEIQMFQNALDFDSLKVRQC
                     MIPRNEIISLSSKESVETLRKKFVETGLSKIMIYQDTKDNIIGFVHSYEMFKNPESIM
                     SVLLPVSIFPETLPARELLTHLTANHRSVAIVVDEYGTTSGMVTIEDIIEEIFGEIDD
                     EHDIDVYLEQKISETEFLFAGRLEVDYLNQKYNLELPESELYTTFAGLIFNHLEDIPH
                     VKEQFTIGSYKITVEQVSGNKIEQVRLVILVNAEN"
     gene            complement(2230..4131)
                     /locus_tag="PKU82_07895"
     CDS             complement(2230..4131)
                     /locus_tag="PKU82_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOZ82832.1"
                     /translation="MSDILHHECGVAMIRLLKPLDYYHKKYGTPFYALNKLYLLMEKQ
                     HNRGQDGAGVANIKFDAEPGTRYISRYRSNDKHAIQDIFSRIYNKFKADVNDDVSKLN
                     DTAWMKKHLAFSGEVWMGHLRYGTYGGNGIEACHPFLRQNNWMTRNLVVAGNFNMTNV
                     DELFENLIRIGQHPKEKADTVTVMENIGHYLDEENERLYYSFKEKGLSKQDISPLIAE
                     HLDVESILKQSSKKWDGGYVICGMMGHGDAFVLRDPAGIRPAYMYADNEVVVVASERP
                     AIQTAFNLSFSDITEIKPGHALIMKKNGNISMPCIKEPVERKSCSFERIYFSRGSDAE
                     IYQERKELGRLLCKSILPAVDNDLRNTVFSFIPNTAEVAFYGMMQGMEDYLREVKKKK
                     IKSLGKEISDEKLNEILSIKPRFEKIAIKDAKLRTFITNDDDRSDMVTHVYDITYGTI
                     RKGIDNLVVIDDSIVRGTTLKNSIIRMLDRLGPKKIIIVSSAPQIRYPDCYGIDMAKI
                     GDLCAFKAAIALLEENNMEHIIEETYMHCKQQLELGVNEMKNYVRDLYKPFSPEHLSA
                     KITSMLKPEDCKADVEIIFQKIEDLHIACPHNKGDWYFTGNYPTPGGNKVVSKAFINY
                     MQGINARAY"
     gene            4575..5516
                     /locus_tag="PKU82_07900"
     CDS             4575..5516
                     /locus_tag="PKU82_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82833.1"
                     /translation="MNKIFAILLLTSGSCIGQNLVPNDGFEIHTSCPYVGNSFSTLSY
                     WFNPSLNLGWGSTPDYYNGCSTLLNDVDVPSNWAGYQFARNGVAYVGIVLWHNQLTFR
                     EYFSVQLISPLVAGTSYHFEMYFNLANQFKYSCSNIGVYFSDTLYTSTINYDPLPFNP
                     QINNINSNYPDTINWSMVSGNYVAHGGENYIIIGNFQNDSNSYPILVDSNAFESGTYV
                     FIDDVSLTVYTGITEQEKIEVIKVYPTLFADKLYLSCEVNESCCVILYDETFRKIIQL
                     PFTKNASLNTGHLAKGMYFYEVRCGSSLCKKGKLVKD"
     gene            5684..6070
                     /locus_tag="PKU82_07905"
     CDS             5684..6070
                     /locus_tag="PKU82_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOZ82834.1"
                     /translation="MKHEDVTRRIIASAMKVHSTLGNGFQEVIYQRAMELEMPFENLF
                     FKREMEMPVHYRNEIIGTRRVDFFVEEIVMVELKAIIKLEDVHLAQAMNYLEAYNMEI
                     GLLINFGSKSLEFKRVHNNKPILKSN"
     gene            6124..7074
                     /locus_tag="PKU82_07910"
     CDS             6124..7074
                     /locus_tag="PKU82_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82835.1"
                     /translation="MRKQICVIILMLTCRICDAQNLVPNGDFEQYSLCPDFPANLALA
                     LFWFNPSTNVQNVSGTSDYYNACANDFYPMNVPGSFTGYQVPHSDSAYAGILLFEHLG
                     VNYREYIEVQITSTLTSNTCYHFEMYISLGDIGKYTTDDIGIYFSDTVVSNINNYAPL
                     PFIPQINNVTGNYPDTTNWLLVQGDYLATGNENYLIIGNFKDDFSIDTLLYNSQGYNA
                     SYIYIDDVSLILTPCTGIEEQNLNSNIKIYPNPLSDILNIKAWGFSEIILYDVAARKL
                     LQQKFTNSTTVNTEQLAKGLYLYEVRNKNGLCKKGKVVKE"
     gene            7579..8913
                     /locus_tag="PKU82_07915"
     CDS             7579..8913
                     /locus_tag="PKU82_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOZ82836.1"
                     /translation="MTQLLFIETFVLGASAIAAIYHLVLYIQQRDKFLLFYSIHLLTL
                     SAYILFKLLSSNYNPFLPTGNLWNYITEETLQGAMVTVYVIFAAITLDVAFSRSNART
                     VMYIFFGFCILFYSYQLYNAFEYGPGVKSIQAYMVTRLSLVGIAAIALIFAWTIRTSI
                     FHRTIIIGSLVYDFSWLVSIISLSTQTTILGLNGIEIYLLGCLLDIIIFSSALGYRVK
                     TMAEEKNELVRKEAEARLAIEKTRTGIAMNLHDDIGSVLSSMSVYGEAAKRALKENNV
                     AKVDVLIDKIGVNARETMDNMSDIVWTINPVNDNGFKLFNRMESFASSVLNAKEIELK
                     FEVGKELYEKGFLMPVRQNVFFIFKEIITNCVKHSGATNVEAIFTLNGSNLLLLVSDN
                     GKGFDTKNNHLKNASGGNGLKNIKERASVLNGTIEVKSSANGTETKLEFPLT"
     gene            9004..9657
                     /locus_tag="PKU82_07920"
     CDS             9004..9657
                     /locus_tag="PKU82_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82837.1"
                     /translation="MAVRVTIFDDNKRLLDSLSVLIDGSPGFQMAGIFENCNNLISDI
                     ERTQPDVILMDIEMPGINGIEAVKMVKHRFPKMNVMMQTAFENDENVFAAICAGASGY
                     ILKNTPPSKILECIIEVYQGGSPMSPSVARKVLGFLQQPKPDEKKETTVENYNLSARE
                     KEILACLVKGMSYKMIAEQCNITYETVRSHMKNIYEKLHVASMTEAVAKAINQKLVS"
     gene            9799..>10870
                     /locus_tag="PKU82_07925"
     CDS             9799..>10870
                     /locus_tag="PKU82_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82838.1"
                     /translation="MKKLLFTLMLLSSFALQAQNFTYNRFFNGGDNSAITLGELAKEV
                     IVQPDGKVISCGYSYDFACNCFHIVMFRVDVCGNIDSTFGTNGTVSYTFEDRNTGLDF
                     LMQANGKIVVVGTQAPSNASSQHNPYIARFLNNGQPDTTFGTMGTTKVIVNPACWEFT
                     HVTEVADGKYLAQNGNYIMRFDSTGAVDLSFGNSGVINYTIPSNVPLVSSWRSVQRSS
                     DKKIISVTAAWLGSGNDMRPLFMCTDTLGQLDSTFGTNGFLLDNNVILPNYQSCHIVL
                     QNDDKILSAHHNAAETEITVARYNTNGSLDTTYGTNGYVNYTAPTSPFRARDVNILSN
                     NNLLVMADAAGLDRQINTFDTNG"
BASE COUNT         3450 a         1934 c         2093 g         3393 t
ORIGIN      
        1 gaaacagaaa agaaaaatct gatagcttat attcagaaat ttcagtctgc aggaccaaaa
       61 ggagtaacaa aactacctca tccttttttt ggaaaaatga cagatgcaga gtgggataag
      121 ctccaatgca aacacctcga tcaccattta agacaatttg gcgtatgaaa tttaggttcg
      181 ttcctgtggt ttttataata tctttgctta tgatgtcgtg cgaaaatgat atagagcaag
      241 tgaaattagt tacaggcaac gttgttgaac ctattgaaag tgctaagggg cttgaaattt
      301 tgtacagcga ctcgggcaaa gtaaaagtgc gcattgcagc cggtgaaatg aatcgctatc
      361 tgcaacccaa gcccatcact gaaatgccta aaggtgttaa gattgatttt tttgatgaca
      421 acctgaaggt gacttccaca ctgacaagcg actatgccgt gcgtgatgat tttaataaaa
      481 acatggaagc aaaaaaaaat gtggaggtgg tgaatgttaa aggagaaaaa ttaatgactg
      541 aatatttaaa gtgggatgaa aaaaccggac ttatttcctc accggaattt gttaagataa
      601 ttactgccga tgaaattatt tacggcaacg ggtttgagtc gaaccaggat ttcagccgct
      661 acaaaatatt taatattaaa ggcattatta acctgaagaa aaatgaaaat actgagaata
      721 cttgagatat cgtggctggt aatagccata ggttcattat gtgccggaat ctataaactg
      781 atttcagcag gactcaatca ggctgtattt atttttgcta tctctattat agcgtttgtc
      841 ttttttatga taagacgaaa acaaagaatt gccatggaga agcagaatgc ttcacgataa
      901 ttacttttgc agcactaaat agatttaatt cctgttaatg atttccgact ttcttcctgc
      961 catacttgca tcacttgctt ttgttgcatt ttattcgggg atagaaattg cctacctgtc
     1021 aagcaataaa tttaaaatag agctcgacag caagcaaggc aaatggagtg gcaaaatttt
     1081 atcttatttc agcaagtcgc cttcacgttt tatctgcact atacttgtag ggcttaatat
     1141 ttcattggtg atttacggta ccaatatgag cggactgctc aatccttatc tgcaacaatg
     1201 gctaccacag caactcaata gcgaaatagg gcgattgata atcgaaacat taataactac
     1261 tttttttctg cttttggcag gagagttttt acctaaaata ttattcagca taaacccaaa
     1321 cgacacatta aattttttta gtggatttat ctggatcagc tatattcttc ttttcccttt
     1381 ggtttggtta attttaaaac ttgcccattt tctgttgcgt aatgttttta aagtagattt
     1441 tgttgaagaa acttccggct tcggcagggt tgacttagat aattttattg aagagataag
     1501 cgaaaatacc ggaggtaaga gcgagcacag taaagaaatt cagatgtttc agaatgcgct
     1561 cgatttcgat agtctgaaag tgcgtcagtg tatgatacca cgcaatgaaa ttatttcgct
     1621 cagcagcaag gagtcagtag agacattaag aaagaaattt gttgaaacgg gattatctaa
     1681 aataatgatt tatcaggaca ccaaagataa catcataggt tttgtacaca gctatgaaat
     1741 gtttaagaat cccgagagca ttatgtcagt actgttgcct gtatctattt ttccggagac
     1801 attgcccgca cgtgagttgc tcacacatct tactgcaaac catcgtagtg ttgccattgt
     1861 tgttgatgaa tatggtacaa cttcaggcat ggtaactatt gaagatatta ttgaagaaat
     1921 ttttggagaa attgatgatg agcatgacat tgatgtttat ctggaacaga aaatttcgga
     1981 aacagaattt ttatttgccg gacgacttga ggttgattat ctgaatcaga aatacaatct
     2041 tgaactaccc gagtcggaat tatatacaac ttttgcaggg ctaattttta atcatcttga
     2101 agacattccg catgtaaaag agcagtttac cattggcagt tacaaaataa cagtagagca
     2161 ggtgagcgga aataaaattg agcaggtaag attagtgatt ttagttaatg ccgaaaacta
     2221 aatttcctct taataggctc gggcattgat tccctgcatg tagttaatga aagctttgct
     2281 caccacttta ttgccaccgg gtgttggata gtttcctgta aaataccaat cgcctttatt
     2341 atgcgggcag gcaatgtgca ggtcttctat tttctgaaaa attatttcta catcagcttt
     2401 gcagtcttcg ggttttaaca ttgatgtgat ttttgccgaa agatgctcag gcgagaaagg
     2461 cttgtataaa tcacgcacat aatttttcat ttcgtttaca cccaattcaa gttgctgctt
     2521 gcaatgcatg taggtttctt caataatatg ctccatatta ttttcttcaa gcaatgcaat
     2581 ggctgctttg aatgcacaca ggtcaccgat ttttgccatg tcaattccat aacaatcggg
     2641 gtagcgtatc tgtggtgctg acgaaacaat tataattttc ttcggaccta aacgatcgag
     2701 cattctgatg atagaatttt ttaatgttgt gccacgcaca atgctatcgt ctataaccac
     2761 taaattgtca atgccttttc tgatggttcc gtaagtaata tcataaacgt gtgtcaccat
     2821 atcgctgcgg tcgtcatcgt tggtgatgaa ggtgcgcagc ttggcatctt taatcgctat
     2881 tttttcaaac ctgggtttaa tggaaagaat ttcattgagc ttttcgtcag aaatttcttt
     2941 tcctaaagat tttatttttt tcttttttac ctcacgcaaa tagtcttcca ttccctgcat
     3001 catgccatag aaggcaactt ctgccgtatt gggtataaag gagaaaacag tgtttcttaa
     3061 atcgttgtca acagcaggca gaatactttt gcaaagcagg cgtcctaatt cttttctttc
     3121 ctgataaatt tctgcatcgc tgcctcttga aaaatatatt ctttcaaacg agcatgattt
     3181 gcgttcaaca ggttctttta tacaaggcat tgaaatgttc ccgttttttt tcattatcag
     3241 tgcatgaccg ggttttattt cggtaatgtc gctgaacgat aaattaaatg ctgtttgtat
     3301 ggcaggacgc tcgctggcta caactaccac ttcattatcg gcatacatat atgcaggacg
     3361 aatgcctgca ggatcgcgca acacaaaagc atcaccatga cccatcatgc cgcagataac
     3421 atagccgcca tcccattttt ttgaagactg ttttagaatg ctttccacat caagatgctc
     3481 ggcaatgagc ggtgagatat cttgtttaga aagccctttc tctttaaatg aatagtataa
     3541 tctttcgttt tcttcgtcaa ggtagtgacc gatgttttcc attacggtaa ccgtgtcggc
     3601 tttttctttt ggatgctgac caatacgaat cagattttca aacaactcat caacattggt
     3661 catattaaaa tttccggcca caactaagtt gcgtgtcatc cagttgtttt gtcgcagaaa
     3721 aggatggcag gcttcaatgc cattgccacc ataggtgcca tagcgcaaat gccccatcca
     3781 cacttcgcca ctgaaggcta gatgtttttt catccatgca gtatcattca gcttcgatac
     3841 atcatcatta acatcggctt taaacttatt gtatattctg gaaaaaatat cctgaatagc
     3901 atgtttatcg ttggaacggt agcggctgat gtagcgtgtt ccgggttcgg catcaaattt
     3961 tatgttggca acaccggctc catcctgtcc acggttgtgt tgcttctcca tcagcagata
     4021 gagtttgttt aatgcataaa aaggagtacc gtatttttta tggtaatagt caaggggctt
     4081 tagcagcctt atcatggcta cgccacattc gtgatgaagg atatctgaca tgaagctaca
     4141 aagatatagc aaaaaggagt acacgacaat agcatttcat aaaatatcag ctgtgccccg
     4201 taaaaagcaa tacagagttt atgtttaact attccgggga ggcgtggtgc taggaaataa
     4261 ttaatcaata gcgccctgaa taatttcatc ttttaggtaa cgctgcccct ctgacagtgt
     4321 gatttaaaca acattttatt ttcttcatcc acaattttca tcagtgacat tttagaaaat
     4381 tttaacattc taaacttttc aactttctaa accttctttt gacaattgcg ataatccgca
     4441 caatgagttg cgaatatttt gtatatttgt ttcataatga ataaacttgt gaattgtgag
     4501 tgttgataat ataaatgtta tcagccattt taacgctccg aacaaactgt actgctaatt
     4561 caaaacgaca taccgtgaat aaaatctttg ccatattatt actaaccagt ggaagttgca
     4621 tcgggcaaaa ccttgttccc aatgacggct ttgaaattca cacatcttgc ccatatgttg
     4681 gaaactcatt cagtacgtta tcctattggt tcaacccatc cttaaatctt ggttggggtt
     4741 ctacaccaga ttactacaac ggatgttcaa ctttattaaa tgatgttgac gtcccatcaa
     4801 attgggctgg ttaccaattt gcacgaaatg gtgtagcata tgttggaata gtgctttggc
     4861 ataatcaact tactttcaga gaatatttta gtgtgcagtt aatatcacca ttggttgcag
     4921 gcacttctta tcattttgaa atgtatttca atttggcaaa ccaattcaaa tattcttgca
     4981 gtaacatagg agtatatttt tcagatactt tatataccag cactatcaac tatgacccct
     5041 taccttttaa tccccaaata aataatataa acagcaatta tcccgataca attaattggt
     5101 caatggttag cggcaattat gttgctcatg gaggagaaaa ttatattatc attggtaatt
     5161 ttcaaaatga ctctaacagt tatccaatat tagtagattc caacgcattt gaatcaggca
     5221 cttatgtttt tattgatgat gtttccttaa ctgtctatac aggaatcact gagcaagaga
     5281 aaatagaggt tattaaagta tatccaactc tgtttgcaga taaattatat ttatcatgcg
     5341 aagtcaacga aagttgttgt gtaatacttt atgacgaaac ctttagaaaa attattcaac
     5401 tcccattcac gaaaaatgct tcactaaaca cagggcatct tgcaaaggga atgtattttt
     5461 acgaagtacg gtgtgggagt agtttatgca agaaaggaaa gttggtgaaa gattgaaatt
     5521 atacagcttc gtggacacag caaaacatgc gcataacagt aagattaaaa gaaataaaaa
     5581 aaagaaggtc cgatagttat cggactttga gaggtaaaca aaattgtctg aaccatgatt
     5641 aaaaggatta gaagattact tgattttaaa caaaagattt gaaatgaagc atgaggatgt
     5701 taccaggcga ataattgcct cggcaatgaa agttcattcc acgctgggaa atggtttcca
     5761 ggaagtaatt tatcaaagag cgatggagtt ggaaatgcca tttgaaaatt tgttttttaa
     5821 aagagaaatg gaaatgcctg tacattaccg taatgaaata attggtacaa gaagagtaga
     5881 tttctttgtt gaagaaattg tgatggttga gttgaaagca attattaaat tggaagatgt
     5941 gcatttggca caggcaatga attatcttga ggcatataac atggaaatag gcttattaat
     6001 aaactttgga agtaaaagtt tagagtttaa aagagttcac aataataaac ccatcctaaa
     6061 atcaaattaa tcaggcaaat catggttcag acaaacagtg ttgctaatac aaacagttcg
     6121 acaatgagaa aacaaatctg cgttatcatt ttgatgttga cttgcagaat ttgtgatgca
     6181 cagaatcttg tacctaatgg agattttgag cagtattcgt tatgtccaga ctttccggct
     6241 aaccttgctt tggccttatt ttggttcaat ccttcaacca atgtccaaaa tgtttctgga
     6301 acctctgatt actataatgc atgcgccaac gacttttacc cgatgaatgt acctggtagt
     6361 ttcactggtt atcaggttcc tcacagtgat tctgcttacg caggaatctt attatttgaa
     6421 catttaggag ttaattatcg cgaatatatc gaagttcaaa ttacttcaac tttaacaagt
     6481 aatacctgtt atcattttga aatgtatatc agtctaggtg atattggtaa atatacaact
     6541 gatgatattg gaatttattt ttcagatact gttgtttcta atattaataa ttatgctcca
     6601 ttaccattta ttccacaaat taacaatgta acaggaaatt atcccgatac aacaaactgg
     6661 cttttagtac agggagatta tttagctaca ggcaatgaaa attatttaat tatcggaaat
     6721 tttaaagatg acttttctat tgatactttg ctttataatt cacaaggtta taatgcatct
     6781 tatatataca tagatgatgt ttccctcatt ctcactcctt gcacgggtat tgaagaacaa
     6841 aatttaaatt caaatataaa aatctatcca aatcctctat cagatatatt aaatattaaa
     6901 gcgtgggggt tttcagaaat tatcctttat gatgttgcag caagaaaatt attgcaacaa
     6961 aaatttacaa actcaacaac tgtaaacact gaacaacttg cgaagggttt atatctttac
     7021 gaggtgcgaa acaaaaatgg tttgtgcaag aaaggaaaag ttgtgaaaga atgaagtggg
     7081 acaacttctc gcgagagaaa aacggctgat aacatctaag actcttgtag gcgcgatcgt
     7141 cagttccgct cgtgccattt agctcttacg acttccagtc gcattcaaaa tttgctatgt
     7201 atttatgtgg tttcatagtc tcaccgattt ttcatcgggg gactatgaaa cggggattgt
     7261 aatttcacct ttcaggttgt gcagcccctg acagtgtgat ttaaatttca accttcaaaa
     7321 ctttcaaact ttccaaacct tcgaaacata ctttgaaata ataaaaaaac attgaagagt
     7381 taatacggtt tgaatccgtt tcatagtcac agcgaagggg cgggagacta tgaaactgca
     7441 aaagtgtttt gtttgttacc gttgccactg caaatattgc tgtgcgaatt agccttgatt
     7501 tgttgactat aaaagttaat cgttagtttt gagcagttgt gcaacaggcg cagaagtttg
     7561 ttgtcaataa ttatttgcat gacacaactt ctttttattg aaacttttgt attgggtgct
     7621 tctgccattg cagccattta tcatttagtg ttgtatatac aacagcgcga taagtttttg
     7681 cttttttaca gcatacattt acttaccctc tctgcctaca ttttatttaa actgttaagc
     7741 agtaactaca atccgttttt accaaccggc aatttgtgga attatataac agaagaaacg
     7801 ttgcaaggtg caatggttac tgtgtatgta atatttgctg ctattacact tgatgttgct
     7861 ttcagcagaa gcaatgcacg tacggttatg tacatctttt ttggtttctg tattctattt
     7921 tacagttatc aattatacaa tgcttttgaa tatggcccgg gagtaaaatc aattcaagcc
     7981 tatatggtaa caaggttatc gcttgtaggc atagctgcca tagccttaat ttttgcatgg
     8041 accatacgta cttccatttt ccatcgcact atcattatcg ggtctcttgt ttatgatttc
     8101 tcatggttgg ttagcattat ttctttgtca acacaaacta ctattttggg tttaaatgga
     8161 atagagattt acctgttagg atgcctgtta gacattatta ttttttcgag tgctttagga
     8221 tatagggtaa aaacaatggc agaagaaaaa aatgaattgg taagaaagga agcagaagcc
     8281 cgtttggcca tagaaaaaac cagaacagga attgccatga acctacatga cgacattggt
     8341 tccgtactga gcagcatgag tgtgtatggc gaagcagcca aacgtgcgct caaagaaaat
     8401 aatgtggcta aggttgatgt tttgatagac aagattggag ttaatgcccg cgaaaccatg
     8461 gacaacatga gtgatattgt atggacaata aaccctgtca acgataatgg atttaaactt
     8521 tttaatagaa tggagtcatt tgcctcatcc gttttaaatg caaaagaaat tgaactgaag
     8581 tttgaagtgg gtaaagaatt atatgaaaaa ggttttttaa tgccggtaag acaaaatgta
     8641 ttttttattt ttaaggaaat tattacaaac tgtgtaaaac attcgggtgc aacaaatgtt
     8701 gaagctatat ttactttaaa tggcagtaac ctgttgcttt tagttagcga taatggaaaa
     8761 ggtttcgaca caaaaaacaa tcatcttaaa aacgcatcag gcggcaatgg tttaaaaaac
     8821 ataaaagagc gtgcatcagt tttaaatgga actatagaag taaaaagctc ggcaaacgga
     8881 acagaaacca aactggagtt cccgctaaca taaaacagcc tatgcccgaa tttggggatg
     8941 acaccatata ttaattgaac ctatttttgt ttcaataaaa tgaaccatta aataaaacac
     9001 aacatggcag tacgtgtaac catatttgac gataataaaa gattgcttga ttcgctatcg
     9061 gtgctaattg atggcagccc gggttttcag atggcaggca tatttgaaaa ctgcaataac
     9121 cttattagcg atatagaaag aacacaacct gatgttattt taatggatat tgaaatgccg
     9181 ggcattaacg gaattgaagc ggtaaaaatg gtgaagcacc gtttcccgaa aatgaatgtg
     9241 atgatgcaaa cagcttttga aaacgatgaa aatgtttttg cagctatatg tgcaggtgca
     9301 tcgggttata ttttaaaaaa cacaccgccc tcaaaaattt tagaatgcat aatagaagtt
     9361 taccagggtg gcagccctat gagcccaagt gtggcgcgta aggtgctggg ctttttgcaa
     9421 caacccaagc ccgatgagaa aaaagaaact actgtagaaa actacaacct ttctgcacgc
     9481 gaaaaagaaa tattggcgtg cttagtaaaa ggcatgagtt ataaaatgat tgccgagcaa
     9541 tgcaacatta cctacgaaac cgtgcgctcg cacatgaaaa acatttacga aaaactgcac
     9601 gtggccagca tgacagaagc tgtagccaaa gccattaacc agaaattagt ttcgtagtaa
     9661 actaactcaa ctctttgtaa aagcgcactc caaaacagtg tgctttttta ttatccccga
     9721 attaggggat tgatttcggc cttgccacaa cccatttttg ctgcatcaaa caaggtcaaa
     9781 ctatataaat aaaatcaaat gaaaaaattg ctattcacct taatgctgct aagcagcttt
     9841 gcattacaag cacaaaattt tacttacaac cgatttttta acggaggcga caattcagca
     9901 ataaccctgg gcgaattagc caaagaagta attgtacagc ctgatggaaa agttataagt
     9961 tgcggttact cttatgattt tgcatgcaac tgctttcaca ttgttatgtt cagagtagat
    10021 gtttgcggta acattgattc tacctttggc acaaacggca cagtatctta cacttttgag
    10081 gaccgcaaca caggtttaga ttttctaatg caggcaaatg gaaaaatagt agtagtgggc
    10141 acgcaggcac caagtaatgc cagcagtcag cataaccctt atatagcacg ctttttaaac
    10201 aacggccagc ccgataccac ctttggaacc atgggtacaa ctaaagtaat tgttaaccct
    10261 gcttgttggg aatttaccca cgttaccgaa gtggcagatg ggaagtatct cgctcaaaac
    10321 ggtaattaca tcatgcgctt tgacagcact ggtgctgttg atttatcatt tggaaatagc
    10381 ggtgttataa attataccat tccatcaaat gttccgcttg tgagctcctg gaggagtgtg
    10441 caacgcagca gcgataaaaa aataatttcg gtaactgctg catggttagg tagcggcaat
    10501 gatatgcgcc cattgtttat gtgtaccgat acccttgggc agttagattc cacttttggt
    10561 accaatggct ttttacttga taataacgtt atcttaccta actatcagtc ttgccatatt
    10621 gtgttacaaa acgatgataa aattctgtct gcacaccaca acgcggcaga aactgaaatt
    10681 acggttgcgc ggtacaacac caatggttct ttagacacaa cttatggtac caacggatat
    10741 gtaaactata cagcgcccac ctcaccattc agagcaagag atgtaaatat acttagcaat
    10801 aacaatttgt tggttatggc cgatgccgca gggcttgacc gtcaaataaa cacttttgat
    10861 acaaacgggg
//
LOCUS       DAOOOL010000085        10833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_561_length_10833_cov_55.989145, whole genome shotgun sequence.
ACCESSION   DAOOOL010000085 DAOOOL010000000
VERSION     DAOOOL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_561_length_10833_cov_55.989145"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..3171
                     /locus_tag="PKU82_07930"
     CDS             208..3171
                     /locus_tag="PKU82_07930"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ82839.1"
                     /translation="MNKRFYLLILVFLFTRSFSARSQSSLSEKSLGIENDFSVEVFNT
                     ESGFPQNSVIDILQSSNGYLWFSTFSGVVRYDGVKFTVFSNNETKGLLSNTVLIIKED
                     KNKNLWLLDDAGHLVKYDGNTFTGFEKSFGNIKISNFCFSEDGTLYVSTTNNEIYTVN
                     GNILDFSTRINQYNIRLITAGKNNSLLIATTGGLFELKNGTIQQIESIPKTEVLSLCY
                     DRDNFLWVGTIAGIYKVSAAKTEIINLPDESEGYSRIFVDRQNRKWIFDGPDGIYLLG
                     TNDFNQISEESGLSSNTVKSVYQDSEDNIWVGTNNAGVNKLTYKIFRTFSVEDGLIAD
                     GVAPIVKSANGAVYVGNNCGGINKIIEGKIIKIPEPKENTCIWSMLEDKDENLWVGTY
                     GGGLFKFKNDKEIFHFNKTNGLSDDVVFALYQDSKGIIWIGTDKSIFTIQHDTLSHFA
                     KDVIKTKVTYFTEDSIGNIWAASNNGLYKINSQKIDLFTTYHGLPSNSIRSLYFDEEK
                     TLWIGTVRGGFSRLKAGKFASFTKIPELSTLDVFCIAEDNQQNLWFTTNKGIYNVKKY
                     DLNNFADQKSATLSFNYYDRKDGLKTTEFNSGFQPNYLKEDPTHFWFPTIKGVAILNT
                     RRIIKNDYIPQIIIESIKADDKEFSPIGNIDLHQKIKTLEINYTAPKFNNPQKVFFQY
                     KLDGVDADWRAATTERTVRYFNLNPGNYTFKIRLYGIFGTEKSLSFYVPMPFYKSNLF
                     LVIVYMTGIALLGLIAYFRIKHIRNQEKKKTELNKRFAEFELKALQAQMNPHFLFNCL
                     NTIKFFITTNNHTAANKYLTKFSKLLRMFLDHSTSESITLEEEINMLRLYIELEQMRF
                     DEGFNFHLQVDEKIDYKNTEIPATLLQPFVENAINHGLVNLNRQGNLTLIFEQKNGCI
                     LGIIDDDGIGRVEAARLKNQSTSQHISRGTQLIDDRIKTLNSIREQHIGIQIIDKEDE
                     QHNSAGTRVIVTIPI"
     gene            3183..3929
                     /locus_tag="PKU82_07935"
     CDS             3183..3929
                     /locus_tag="PKU82_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ82840.1"
                     /translation="MLQTLIVDDEKRGRELLKMILATNCPEVKVTGEASNIKEAYQMI
                     LQNEPDLVLLDIEMPGGTGFDLLSKFDEVNFDVIFITAFDQYAIKAIKFSAMDYILKP
                     VDEEELIKAIKRAEENRNRKNNKEKVNTLISNVQKSSNHQKIGLTSGEGLEFIEIKNI
                     LRCEADGKYTSVFLTDGKKLLVSKNLKEFEDLLTEYNFFRIHHSHLVNLDCIKKYQSG
                     RGGYVVMSDGSTITVSQRKKDDFLSSLKKI"
     gene            4008..4469
                     /locus_tag="PKU82_07940"
     CDS             4008..4469
                     /locus_tag="PKU82_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOZ82841.1"
                     /translation="MKHLFLVLCIAATAFVSCKSDQQKKTIEPEMVNNPASAEPENAD
                     TSLLPKFDFESDNHEFGSITEGEIVSYNFKFKNSGKAPLIITQASASCGCTVPEYSKD
                     PIAPGQEGFIKVTFNSEGKHGMTSKTVTLLANTVPNTKVLTISADISKKDK"
     gene            4466..4789
                     /gene="yajC"
                     /locus_tag="PKU82_07945"
     CDS             4466..4789
                     /gene="yajC"
                     /locus_tag="PKU82_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOZ82842.1"
                     /translation="MMYLTILLQSQGNMAMQQFLMLGLIIVIMYFFMLRPQMKKMKVE
                     KQFKESINKGDKVVTIGGVHGKITEVGETFFMIEIDNNVKIKIDKTAVSAEASKQYRK
                     TDDKK"
     gene            4802..5749
                     /locus_tag="PKU82_07950"
     CDS             4802..5749
                     /locus_tag="PKU82_07950"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOZ82843.1"
                     /translation="MNIFKNIRLKGNASLPVFFICVFVSLVFWILYSLNKNYVYPVTY
                     TVTLQNTPFSKQVISEIPQQVNVTLRSRGFELFWYLIRNRERNLTVDLKKYSVNNEKL
                     GISLQSEFRNQNQNRLKEMDVVTTLPDSVWLYLSSKYMKTVPVVTQFTYSIKKNFGLS
                     GKVSIAPDSITISGEKSQLDGVNEIKTVATDYDNLDRDLKATLSLISPAGKVNLSQKE
                     IKIHIPVEEFIEKKIPVVLNYSINGSGKVILIPEKAELIIQVPLKRYEQLSADSFQVT
                     VSAKRYLKEGQLAVELIHKPQGINVSSIVPSTVNYFIER"
     gene            5749..6330
                     /gene="coaE"
                     /locus_tag="PKU82_07955"
     CDS             5749..6330
                     /gene="coaE"
                     /locus_tag="PKU82_07955"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386593.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOZ82844.1"
                     /translation="MKKIGITGGIGSGKTFVANIFKTLGIPVYDADTAAKQLMNHEPL
                     RSQLKQLFGNSIYNEHDELNRPVLASLIFNDEKKLDAVNALVHPAVAGHFSEWCNNQK
                     ADYILKEAAIMFESGANKELDAVICVSAPETVRIERAMKRDNTSAEKIKAIIAKQLNE
                     EDRNKRCNYLIKNDGTEPLLPQVLKIHDLLKAK"
     gene            6466..6801
                     /locus_tag="PKU82_07960"
     CDS             6466..6801
                     /locus_tag="PKU82_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOZ82845.1"
                     /translation="MLHNFDIILKMMKEYKASRMSEGNKVLPNKIRIDNDGITFRIPG
                     VFSGEEKTIPFSRISSVEVNTPLVGFSSITINTTGEGAIVATGFYKEDVLEIKKIVLE
                     KINVFQNKM"
     gene            complement(6805..8454)
                     /locus_tag="PKU82_07965"
     CDS             complement(6805..8454)
                     /locus_tag="PKU82_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOZ82846.1"
                     /translation="MQSDQKKNFQPLWYALLITVGIVIGVSLNSSFKGGTNILFSRSA
                     SGGFNKINDVVNYIRQEYVDTINQQELVNSSITQILQNLDPHSSYIPADELKSVNEPL
                     EGNFEGIGIEFHIQYDTIMVVSTIAGGPSETIGLRPGDRIVEVDKKKVAGIGIKNEDV
                     FKYLRGQEGTKVALKVKRSGIEKLLDFTIVRGKIPIRSIETSFMANANTGFIKISRFS
                     ATTYDEFMEAVEALKKQGMQNLILDLRGNPGGYLDAATMLADEFLPDKKLIVYTEGKA
                     RPRKNYYATEKGDFEKGKVFVLIDEGSASASEILAGALQDWDRAEIIGRRSFGKGLVQ
                     EQTLFPDGSAMRLTVARYYTPTGRSIQKPYKNGYMAYEEEVYDRFKHGEMVNADSTHF
                     NDSLKYTTPGGKIVYGGGGIMPDVFIPIDTTADNEFARSVFGLGLVSRFSYDYTDKHR
                     NKLNTYKSIDEYIKNFSADQRLLSDFFVYAIDNKVRPDEKSIHASRQLIANQLKANIG
                     RLLFGNDAFYRVVLSDDKAFNAALQQAESTAQSKVKESEAK"
     gene            complement(8464..8937)
                     /locus_tag="PKU82_07970"
     CDS             complement(8464..8937)
                     /locus_tag="PKU82_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOZ82847.1"
                     /translation="MGSPGFDDIYMDLAKTLALKSHCVKMQVGAVLTKDTRIISLGYN
                     GPPAGTHNCDTEWPDDGCPRDSKGGCSLAIHAEQNAILYAAKNQVSVEGATLYVTLSP
                     CLACAKVVFTSGIKKVIFLNSYAQYKGIKSDEGVDFLRKFNVEVQQYTPGTVSGT"
     gene            complement(8982..9068)
                     /locus_tag="PKU82_07975"
     tRNA            complement(8982..9068)
                     /locus_tag="PKU82_07975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9032..9034),aa:Leu,seq:caa)
     gene            9178..10113
                     /locus_tag="PKU82_07980"
     CDS             9178..10113
                     /locus_tag="PKU82_07980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOZ82848.1"
                     /translation="MAREVKLFSGEATRYLADNIALSYGLPLGDVIVSHFSDGEFSPY
                     FDETVRGCDVFIIQSTFAPTNNLFELLMLIDAAKRASAHYITAVIPYFGYARSDRKDK
                     PRVAIASKLVANLLTAAGVTRVMTMDLHAPQIQGFFDIPVDHLDASSIFVPYIKNLNL
                     PNLLIAAPDMGGVHRAREYAKFFNAEMAICDKHRKRANEVASMQVIGDVAGKDVILVD
                     DIVDTAGTLSKAAELLIEKGASSVRAFCTHAVLSGNAYETIENSKLTELIVTDTIPLR
                     KKSAKIVQLSTAELFSYAIRRVYEYSSISNLFVKS"
     gene            10135..10770
                     /locus_tag="PKU82_07985"
     CDS             10135..10770
                     /locus_tag="PKU82_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOZ82849.1"
                     /translation="MKSLTIKGTKRVGLTKQELKKLRADGKVPCVLYGGKDNVHFSTT
                     ALELRDLVYSPSVYTVNLDVNGEQHQAVMQDIQFHPVTDKIQHIDFLLLQNNKPVSIA
                     VPVTVKGSAEGVKQGGKLIQKMRKMQISALPANLPDAIEIDVTTLGIGQAVKVANIQR
                     DNITLLDSPNNIIVAVRTTRNVVEETPAAAAAAAPAAAKPAAAAPAAKPAK"
BASE COUNT         3584 a         2048 c         1986 g         3215 t
ORIGIN      
        1 cttttacgaa tgtaacataa gttggcaata aacaaaagcc ggaaagtaac aattgagtat
       61 tttagcgata agattaagtt aaaaagcatg acaatacttt ttatatgaca cagtttcaca
      121 ttcaattttt ggctatctat taactgcaac tgttactttt taccacttaa taactgattt
      181 aatgcttaat ttggcagcat aatcaatatg aataaacgct tttatttact aatcttagtt
      241 tttcttttta caagatcttt ttctgccagg tcgcaatcat ctttgtcaga gaaaagcctt
      301 ggtattgaaa acgatttttc tgttgaagta tttaatactg aaagcggttt cccacaaaac
      361 tctgtaattg atattctaca atcttcaaat ggctatttat ggttttctac ttttagtggt
      421 gtagttcgtt atgacggagt aaaatttaca gtgttcagca ataatgaaac taaaggatta
      481 ctatccaaca ctgtattaat tattaaagaa gataaaaata aaaacctatg gctgctggac
      541 gatgccggtc atcttgtaaa gtatgatgga aatactttta ccggctttga aaaatcattt
      601 ggtaatataa aaatatctaa tttctgtttt tctgaagatg gaacactcta tgtttccaca
      661 acaaacaatg aaatctatac cgtaaacggc aacattctcg attttagtac acgcatcaat
      721 caatacaaca ttcgtttaat tacagccggt aaaaacaaca gtttattaat agccacaaca
      781 ggtggtttat ttgagttgaa aaatggaacc attcaacaga tagaaagtat tccaaaaacc
      841 gaagttttaa gtctctgcta cgaccgcgac aattttcttt gggttggaac aatagccgga
      901 atttataaag taagtgcggc aaaaactgaa ataattaatc ttcctgatga atccgaaggt
      961 tattcaagga ttttcgttga ccgacagaac agaaagtgga tttttgatgg cccagatggt
     1021 atatatcttt taggtacaaa tgattttaat cagatctctg aagagtctgg cttatcatcc
     1081 aacactgtca aatctgttta tcaggattca gaagacaata tttgggtggg aaccaacaat
     1141 gcaggagtaa acaagcttac ttataaaata ttcagaacat tttctgttga agacggattg
     1201 attgctgatg gtgttgcgcc tattgttaag tcagccaatg gtgcagttta tgttggcaac
     1261 aattgtggtg gtattaataa aattattgaa ggaaagatta ttaaaattcc tgagccgaaa
     1321 gaaaacacct gtatttggtc aatgcttgaa gataaagatg agaacctgtg ggttggaact
     1381 tatggtggag gattatttaa atttaaaaat gacaaagaaa ttttccattt caacaaaaca
     1441 aacggacttt ctgatgatgt tgtttttgca ttatatcagg acagtaaggg aataatttgg
     1501 attggaaccg ataaaagtat tttcactatt cagcatgata ctttaagtca ctttgcaaaa
     1561 gatgttatta aaaccaaggt gacatatttt accgaagatt caattggaaa catctgggct
     1621 gcaagtaaca atggcttata taaaatcaat tctcagaaaa ttgatttatt tacaacctat
     1681 catgggttac catctaactc tataagatcg ctttattttg atgaggaaaa aactttatgg
     1741 ataggcacag taagaggtgg tttttcaaga ttaaaagcag ggaaatttgc gagctttacc
     1801 aaaatacctg aactatcaac tttagatgtt ttttgcattg cagaagacaa tcagcaaaac
     1861 ctgtggttta caacaaataa aggtatttac aatgtaaaaa aatacgacct caacaatttt
     1921 gctgaccaga aatcagcaac actttctttt aattattacg acagaaaaga cggattaaaa
     1981 actacagaat ttaattcggg gtttcaaccc aattatctga aagaagatcc cacacacttt
     2041 tggtttccaa caataaaggg tgttgcaatt ctaaatacaa gaagaattat caagaacgat
     2101 tacatcccac aaataataat tgaaagcatt aaagcagatg ataaagaatt ttcaccaatt
     2161 gggaatattg atctgcacca aaaaataaaa acgctggaaa taaattatac agcaccaaaa
     2221 ttcaacaacc ctcaaaaagt attttttcaa tataagttag atggtgttga tgccgactgg
     2281 agagcagcaa ctacagaacg cacagtgcgt tattttaatc tgaatcccgg gaattatact
     2341 tttaaaatta ggttatacgg catttttggt acagaaaaat cattgagttt ttatgtgcca
     2401 atgccatttt acaaaagtaa ccttttctta gtcattgttt atatgacagg cattgcacta
     2461 cttggtttga tagcttactt tagaattaaa catatacgca atcaggaaaa aaagaaaaca
     2521 gaacttaaca aacgctttgc tgaatttgaa ttaaaagcac ttcaggcaca aatgaatccg
     2581 cattttttat tcaactgtct caatacaatt aaatttttta ttacaaccaa caaccataca
     2641 gcagccaata aatacttaac aaaattctcg aagttgttac gtatgtttct cgatcattcc
     2701 acttccgaga gtatcacact tgaagaagaa ataaatatgc tcagacttta tattgagctg
     2761 gaacaaatgc gttttgacga aggttttaac ttccatttac aggtggatga gaaaattgat
     2821 tataaaaaca ctgagatacc ggctacctta cttcagccct ttgttgaaaa cgccattaac
     2881 catggattgg tgaatttaaa ccgacaaggc aatttaacgc ttatctttga gcaaaaaaac
     2941 ggatgtattc ttggtataat tgacgatgat ggcattggca gagttgaagc agcgcgatta
     3001 aaaaaccagt caacatcaca acatatatca agaggaacac aattgattga cgacagaata
     3061 aaaacattaa acagcatacg cgaacagcac attggcattc agattataga caaagaagac
     3121 gaacaacata attcagccgg aacacgtgtg attgttacca taccgattta atacaaaaca
     3181 agatgttaca gacattaata gtagatgatg aaaaacgcgg ccgcgagctg cttaaaatga
     3241 ttcttgccac caattgcccc gaggtaaaag ttaccggtga agcatcaaat ataaaagaag
     3301 cctatcagat gattcttcag aatgaacccg atctggtttt attggatatt gaaatgcctg
     3361 gtggcacagg gttcgacctg ctctctaagt ttgatgaggt aaactttgat gtcattttta
     3421 tcacagcatt cgatcaatat gccattaaag caataaaatt ttctgcaatg gattatattc
     3481 tgaaacccgt tgacgaagaa gaattgatta aagccattaa acgtgctgaa gaaaaccgaa
     3541 accgtaaaaa caataaagaa aaagtaaaca ccctgatttc aaatgttcag aaaagcagca
     3601 atcatcagaa aatcggactg acatcgggtg aaggacttga gtttattgaa ataaaaaata
     3661 ttttacgttg cgaagctgat ggaaaatata cttccgtttt tcttactgat ggcaaaaaac
     3721 tattggtaag taaaaactta aaagagtttg aagacctact cacagaatac aactttttca
     3781 gaattcacca ctcacattta gtaaacctcg actgtattaa aaaataccaa agtggccgtg
     3841 gcggctatgt ggtgatgagt gatggcagca ccattactgt ttctcaacga aaaaaggacg
     3901 acttcttaag cagtctgaag aaaatctgat aaataagctt ttgtcgaaaa tactgtttgc
     3961 tgcttaggta acttagcctt actttcgcag caaaattgag ttatagtatg aaacacctct
     4021 ttttagtttt atgtattgct gccactgcat ttgtatcatg caagtccgat cagcaaaaga
     4081 aaaccataga acctgaaatg gtaaacaacc ctgcaagtgc agagcctgaa aatgcagaca
     4141 cttctctttt acccaaattt gattttgaaa gcgacaatca cgaatttggt tctattaccg
     4201 aaggcgaaat tgtatcctac aattttaaat ttaaaaattc cggtaaagct ccgttaatta
     4261 tcacacaggc aagtgccagc tgtggctgca ccgttcctga atactcaaaa gaccctattg
     4321 ctccagggca ggaaggattt ataaaagtta cctttaatag tgaaggtaaa catggcatga
     4381 caagtaagac agtaacctta cttgccaaca ccgttccaaa cacaaaagtt ttaaccatta
     4441 gtgcagatat ttctaaaaaa gacaaatgat gtatttaacc attctacttc agtcgcaggg
     4501 caacatggcc atgcagcaat tcctcatgtt aggattaatt attgtaatca tgtatttctt
     4561 tatgctgcga ccacagatga aaaaaatgaa agtcgaaaag caattcaaag aaagtattaa
     4621 taaaggtgat aaagtagtta ccattggtgg cgtgcatgga aaaattactg aagttggtga
     4681 aacttttttt atgattgaga ttgacaacaa cgtaaaaatc aaaattgata aaactgccgt
     4741 ttctgcagaa gcatcaaagc agtaccgcaa aacagacgat aaaaaataaa aagccttaca
     4801 cttgaatatt ttcaaaaata tcaggttaaa aggcaatgca tcattgcccg tattttttat
     4861 ttgtgttttt gtgtcgcttg ttttctggat tttatactca ttgaacaaga attatgtata
     4921 tcctgtaact tatactgtta ctcttcagaa tacaccattc agcaagcaag tcatcagcga
     4981 aatacctcaa caggtaaatg taacacttcg ttcaagaggg tttgagttgt tttggtattt
     5041 aatcagaaac cgcgaaagaa acttaacagt tgatctgaaa aaatattctg taaacaatga
     5101 aaaacttggt atcagtttac aaagtgaatt cagaaatcag aatcaaaacc gtttaaaaga
     5161 aatggatgtt gttacaacac tacccgattc agtgtggctt tatctttcgt ctaagtacat
     5221 gaaaacggtt cccgtagtta cgcagttcac atattccata aagaaaaact ttgggttgtc
     5281 aggtaaagtc agcattgcgc ccgacagcat taccatttcc ggagaaaaaa gtcagcttga
     5341 cggtgtgaat gaaataaaaa ctgttgctac agattacgac aatcttgacc gtgacttaaa
     5401 ggctacccta tcattaattt cacctgccgg caaagtcaat ttatcgcaga aagaaataaa
     5461 gattcacatt cctgttgaag aatttatcga gaaaaaaatt cctgttgttt taaactattc
     5521 tattaacggc agtggtaagg ttatactcat tcctgaaaaa gcagaactca tcattcaggt
     5581 gcctttaaaa cgctatgaac aacttagtgc tgactcgttt caggtaacgg tttctgcaaa
     5641 aagatattta aaagaaggtc agttggccgt tgagttaatc cacaagccac aaggaataaa
     5701 tgtttcatct atagttccgt caacagtaaa ctattttatt gaacgctgat gaaaaaaatt
     5761 ggcataaccg gtggcatagg ttcgggaaaa acctttgtag ctaatatttt taaaacttta
     5821 ggcataccgg tttatgatgc agacacagca gccaaacaat taatgaacca tgaaccattg
     5881 cgcagccaac tcaaacaact ttttggcaac agtatttata acgaacatga tgaactcaac
     5941 aggcctgttt tggcttcgct tatttttaat gatgaaaaaa agttagacgc tgtcaatgcc
     6001 ttagtgcatc ctgcagttgc aggacatttt agtgaatggt gcaataatca aaaggcagat
     6061 tatattctaa aagaagctgc aataatgttt gagtcaggcg caaacaaaga gttagatgct
     6121 gtaatttgtg tttctgcacc tgaaacagta agaattgaac gcgccatgaa acgcgataac
     6181 acatcagcag aaaaaataaa agcaattatt gccaaacaac tgaatgaaga agatcgcaac
     6241 aaacgttgta actatcttat aaaaaatgat ggcacagagc ctttactgcc gcaggttctg
     6301 aaaattcatg atttgttgaa ggcaaaataa aaaggccgtc tgatgcagac agccctttta
     6361 cctaacctaa aactaacctg aaaaactcct tattttctgt gttacggtat ttacaataac
     6421 gcaaatcaga tgccactatt ttaatttttt atgaatgata tatgaatgtt gcataacttt
     6481 gacattattc taaaaatgat gaaagagtat aaagcatccc gaatgtcgga agggaataaa
     6541 gttttgccca acaaaataag aatagacaat gatggaataa cattcagaat accgggtgta
     6601 tttagtggtg aagaaaaaac tattcctttt tcaagaattt catctgttga agttaacaca
     6661 ccgttagtag ggttttcttc catcaccatt aacacaacgg gcgaaggtgc tattgtagca
     6721 acaggctttt ataaagaaga tgtgcttgaa ataaaaaaaa ttgtactcga aaaaattaat
     6781 gtttttcaga ataagatgta aaagttattt cgcctctgac tctttaacct tactctgcgc
     6841 tgtactttct gcttgttgca atgcagcatt aaacgctttg tcatctgaca atacaacacg
     6901 ataaaaagca tcgtttccaa acaacaacct tcctatgttt gctttaagct gattggcaat
     6961 tagctgtctt gaagcatgaa tacttttttc atcaggtctt actttattat caatagcgta
     7021 aacaaaaaag tcgctgagta aacgctgatc ggcagaaaag ttttttatgt attcgtcaat
     7081 acttttatat gtgttgagtt tatttctgtg cttatcagta tagtcgtacg aaaaacgact
     7141 taccaatcct aacccaaaaa cagaacgcgc aaactcatta tcggcagtgg tgtcaattgg
     7201 aataaacaca tcgggcataa tgcctccacc accataaaca attttaccac cgggagtggt
     7261 gtattttaat gagtcgttaa agtgtgtgct gtctgcattt accatttcgc catgtttaaa
     7321 ccggtcatac acttcttctt cgtaagccat gtaaccgttc ttataaggtt tctgtatgct
     7381 gcgccctgta ggtgtataat aacgtgccac ggtaagtcgc atagccgaac catcgggaaa
     7441 caatgtttgc tcctgcacca atcctttgcc gaatgacctg cgaccgataa tttctgctct
     7501 gtcccaatcc tgcagagcac ctgcaagaat ttctgatgcc gaagcagagc cttcatctat
     7561 cagaacaaaa acttttcctt tttcaaaatc gcctttctct gtagcataat aattttttcg
     7621 tggtcgcgct ttcccttctg tgtaaacaat caacttttta tctggcaaaa attcatcggc
     7681 aagcatggtt gctgcatcaa ggtatccacc ggggtttcct cttaaatcaa gaataagatt
     7741 ttgcattcct tgctttttca atgcctccac agcctccata aactcatcgt aggtggtggc
     7801 agagaatctc gaaattttaa taaagcctgt gttggcattt gccataaatg atgtttctat
     7861 gctgcggata ggaattttac ctcttacaat ggtaaagtcg agcaatttct ctataccact
     7921 acgtttgacc ttcaaggcaa ccttggttcc ttcctgtccg cgcagatatt taaacacgtc
     7981 ttcgttttta atgcctatgc ctgcaacttt tttcttatct acttcgacaa tgcggtcacc
     8041 ggggcgtagt ccaatagttt cagaagggcc acctgcaatg gttgacacta ccataatagt
     8101 atcatactgt atgtgaaact ctattcctat gccttcaaag tttccttcca gcggctcgtt
     8161 aaccgatttc agttcatcag caggaatgta ggaagaatgt gggtcaaggt tttgaagtat
     8221 ttgtgtaatg ctgctgttaa ccaactcctg ttgattgatg gtgtcaacat attcctgacg
     8281 gatgtagttt acaacatcat taattttgtt gaaccctcct gatgcactgc ggctgaacaa
     8341 aatatttgtg ccgcctttaa atgatgagtt taatgaaaca ccaatgacaa tgcctacggt
     8401 aatcaataaa gcataccata atggttgaaa gttttttttc tgatcagact gcatgaaaag
     8461 atattatgta ccggaaacag ttccgggagt atattgttgc acttctacat taaattttct
     8521 taaaaaatca actccttcat cagattttat gcctttatac tgcgcatagg aatttaagaa
     8581 aatcactttt tttattcctg aagtaaacac caccttagca caggcaaggc atggcgaaag
     8641 tgttacatag agagtagccc cttctaccga aacctgattt tttgcagcat aaagaattgc
     8701 attttgttcg gcatgaatgg ctaaggagca accacccttg ctgtcgcgcg ggcaaccatc
     8761 atcaggccac tccgtatcgc aattgtgtgt gcccgcaggc ggaccattat atccaagaga
     8821 gatgatgcgc gtatctttgg tgagcaccgc tcccacctgc attttaacac agtgcgattt
     8881 taaggcaagt gtctttgcca gatccatata aatatcgtca aaaccgggtg aacccatagc
     8941 tgattatatt aaaaagctaa gccctcagtg tgagggcttg ctggtacccg gagccgggat
     9001 cgaaccggca tgaggtttag ctcactggtg tttgagacca gcgcgtctac caattccgcc
     9061 atccgggtaa attctattca aaaaacgagg ctgcaaaagt attaatttta cacaatccat
     9121 ttgacctgag cgaaattttt cctttctttg cagccctttc agaatccccc cttatctatg
     9181 gcaagagaag tcaaattgtt ttccggtgaa gctacacgct atctggctga taacattgca
     9241 cttagttatg gtttacccct tggcgatgtc attgtttcgc atttcagcga tggcgagttt
     9301 tctccctatt ttgacgaaac agtgcgtggt tgtgatgtat ttatcattca gtcaaccttt
     9361 gcccccacca acaatttgtt tgaattgctc atgcttattg atgctgccaa gcgtgcttct
     9421 gcacactaca tcactgccgt aattccttat tttggttatg cccgcagcga ccgcaaagac
     9481 aaacccagag tagccattgc cagtaaatta gtagccaatc tgctcactgc tgccggtgtc
     9541 acccgtgtga tgacaatgga cttgcatgca cctcagattc agggattttt tgacataccg
     9601 gttgaccact tagatgcctc ttcaatcttt gttccttata tcaaaaactt aaacctgccc
     9661 aacctgctca ttgcagcacc ggacatgggc ggtgttcatc gtgcccgcga gtatgcaaaa
     9721 ttctttaatg cagaaatggc catctgcgac aaacaccgca aacgcgccaa cgaagtggca
     9781 agcatgcagg ttattggtga tgttgccgga aaagatgtaa tcttagttga cgacattgtt
     9841 gacactgccg gaacattatc aaaagctgcc gaacttttaa tagaaaaagg tgccagctct
     9901 gtgcgtgcat tctgcacaca tgctgtactg tcgggcaatg cttacgaaac gattgaaaac
     9961 tcaaaactta ctgagttaat tgttaccgat actattccat tgcgtaaaaa gtcagctaaa
    10021 atagttcagc tctcaacagc agagctattc tcttatgcta taagaagagt gtatgaatac
    10081 tcttcaataa gcaatctgtt tgttaaatct taaatttata aattatatat tacaatgaaa
    10141 tcattaacga ttaaaggtac caaaagagta ggccttacca aacaagagtt gaaaaaactc
    10201 cgtgccgatg gtaaagtacc atgcgtgtta tacggtggga aagacaatgt gcacttcagc
    10261 acaacggcac ttgaactaag agacttggtt tattctccaa gcgtttacac ggttaatctc
    10321 gatgtaaatg gagaacaaca tcaggctgtt atgcaggata ttcagtttca tcccgtaact
    10381 gacaaaattc agcacattga cttcttatta cttcaaaaca acaagcctgt ttctatagca
    10441 gtgcctgtta cagttaaagg aagtgcagaa ggggttaaac aaggtggaaa attgattcag
    10501 aaaatgcgca aaatgcaaat cagtgcattg cctgcaaatc tacctgatgc tattgaaatt
    10561 gatgttacaa cattaggcat tggtcaggca gttaaagttg ctaatattca gcgcgacaac
    10621 attacattac ttgattctcc aaacaacatt attgttgcag taagaactac aagaaatgtg
    10681 gttgaagaaa ctcctgctgc ggctgccgct gctgcacctg ctgccgctaa accggctgct
    10741 gctgcacctg ctgctaaacc ggctaaataa tttttttgtc aatctattga agccgggtat
    10801 tccaatatcc ggcttctttg tttaaaataa ata
//
LOCUS       DAOOOL010000086        10695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_571_length_10695_cov_44.819361, whole genome shotgun sequence.
ACCESSION   DAOOOL010000086 DAOOOL010000000
VERSION     DAOOOL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_10695_cov_44.819361"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKU82_07990"
     CDS             complement(<1..596)
                     /locus_tag="PKU82_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HOZ82850.1"
                     /translation="MDASKTLEAATSRLRKYIESHHYKGYDPYDALKSPFFKLPLLNK
                     NKLIRFGAQQLVKRSPLNLRPLLFVPKGLNPVTLGLCIQAYAQLINVNQQEKNILNQN
                     IAKLIDELEKLIPKGFHGACWGYDFDWEARYANIPAYQPTVVATGIITNALFIAWQQT
                     GNQKAFQLCKSAADFVMKDLNRTVDANGHFCFSYSPFD"
     gene            complement(601..2727)
                     /locus_tag="PKU82_07995"
     CDS             complement(601..2727)
                     /locus_tag="PKU82_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOZ82851.1"
                     /translation="MEQLTQNLKDGKMQLLEVPFPVLSSGQVLVRNHYSLISAGTEGK
                     TVKDARLSYIGKARARKEEVKKVIDAAKTFGILNTYKMVMNKLDAPSSLGYSTAGEII
                     AVAPDVTEFRVGDLVACGGNSAVHAEVIAVPVNLCVKLNDKIALQHACFTTLGSIALQ
                     GIRQADLRLGENCVVIGLGLVGQLTVQMLKAGGIKTIGIDIDQRMVDLALHTDIDAAF
                     TRNSEDLESSINNITNGYGTDAVIITAGTDSLDPVDLAGALCRRKGKVVIVGAVPTGF
                     KRPNYFKKELDLRMSCSYGPGRYDTEYEEGGIDYPYDYVRWTENRNMAAFVDLIATGK
                     IHLSNLLTHTFDFKEAPAAYDLIMNKTEPFVGMVLKYDTNKPLQQKVNLSNPFYTKSD
                     VVAGMIGAGSFGQNFLLPALKNTPAKLSGIVTARPNNARNIADKNQFSFASGNAQDIF
                     SNGDINTVFIATRHETHAGYVMQALQAGKNVFVEKPLCLTEEELVQIQHEFTKQNCRV
                     MVGFNRRFAPMIQTIKKTFSSGNPIAINYRINAGVIPADHWVHDLKVGGGRIVGEACH
                     FIDLCMFLAGSKLTTISAVALNDAQKLNDTVCFNLQFENGSTASVSYFSNGNKNINKE
                     YLEVFGSGIVAVLDDFKTLTVFGKSEKKYTGNQDKGHQNEVKAFVDSIVQGKPAPIPF
                     EETVLSTLATFRALQSIASNGERIIL"
     gene            complement(2747..3706)
                     /locus_tag="PKU82_08000"
     CDS             complement(2747..3706)
                     /locus_tag="PKU82_08000"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOZ82852.1"
                     /translation="MKKVLVTGGAGFIGSNLCDALLTNDLYEVHCLDVFDDFYNPAVK
                     RNNISEALKSKNFFLHEGDIRDTDFLEKLFQQPFDIIVHLAARAGVRPSIEKPLLYED
                     VNIKGTVNLLEKAKEKNIKQFVFAGSSSVYGINPNTPWSENDFVLKPISPYASTKVSC
                     ELTGHTYAHLYGIRFISLRFFTVFGPRQRPDLAIHKFFKLIRQGTPIPFFGNGETSRD
                     YTFVGDIVNGIIAAMHYDKSNYEIINLGNNRTINLNMLIAAIEKEAGKKALLEKLPDQ
                     PGDVPHTFADISKAKELLNYSPATTLEEGLIAFRKWFDQNHST"
     gene            complement(3718..4827)
                     /locus_tag="PKU82_08005"
     CDS             complement(3718..4827)
                     /locus_tag="PKU82_08005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ82853.1"
                     /translation="MSDKKYTVIFLGKLPPPYIGPSVACEIILNSKLKDEFNLIHLDL
                     SDKRDINTLAKLDLTNFYLAFKQYFQLFWYCIRYRPDLVYVPAGQTNVGYTRDAGYIL
                     IAKLFGIKVLTHLRGGYFLNWYNESGKTMKSFIRFVHKKVNGQIVLGNNLRKLFNWIV
                     PDEKIFVIPNGGNFPVNYNERNNNKVKVLFLGNYVRTKGVLEALYAAPLVYEKNKNVE
                     FVFAGEWREADVKTDFKKFLQQHPELPVINHGPVKGDKKFSILNEADIFVFPTYYRNE
                     GHPWVTVEAMAAGLPVISTDHAAISESVHDGKNGYLVEKKNIQQLAEKIIILANDKDL
                     RNKMGAESRRIYLENLTEEKMVERMGHAFRSVIEN"
     gene            complement(4824..6116)
                     /locus_tag="PKU82_08010"
     CDS             complement(4824..6116)
                     /locus_tag="PKU82_08010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ82854.1"
                     /translation="MTASTNINLKKLLIIILLAGFVAKIAIGFHLKDSFLNRGNSKTN
                     LNALACNLYVNGEYSLVQGIPSVDYEPLYPALMSISYAITGYNWLGLTILQAVLHLIT
                     SLLLFKIGTRLFHPIAGFFAACYHAFYPYLFTYSFSIYDTTLFVFLLVLTIELLLTEK
                     LSSKRLVLAGIIIGLAFLTRATIITFLPGLFLYVFYRYFKSQNVTAAFISCSLIVAGC
                     LLAMAPWLVRNYSLTGKLLISTHGSFGMWQGNNPLSFEYLKANKSLDEIYRLNPPLPI
                     YAQYPLAARTPDEAVKVSAAYQQEAVNWIKNNFPEFVSMGWLKAEKLWSWNRNPDSKS
                     VKFGSNEGRKSVYLISYLPILLLFPFGLILLFKKHPVEAFTLLLILLAFTGAHSIAIG
                     FTRARIPIDFFLMLCSGITITWLLQRFFPSLYLKFKSS"
     gene            complement(6214..6999)
                     /locus_tag="PKU82_08015"
     CDS             complement(6214..6999)
                     /locus_tag="PKU82_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOZ82855.1"
                     /translation="MNFELSQMPSRSTKPRENGLTMVMDKGLSLRETEDFISSSGEFT
                     DLVKLGFGSSLITPNLDKKLALYKEAGIPVYFGGTLLEAFLIRDEFDKYLKVLSKYKM
                     EYAEISDGSIVIPHKIKCDYIKKLAKHVTVISEVGSKEEGIIIRPNIWIQMMERELEA
                     GVWKVIAEARESGTVGIYRPNGKAHVMLINKIISRVPADKIIWETPQKSQQVYFIKQF
                     GTNVNLGNIAPSEVIALESLRLGLRSDTFFHFLDKESINRFTN"
     gene            complement(7005..8417)
                     /locus_tag="PKU82_08020"
     CDS             complement(7005..8417)
                     /locus_tag="PKU82_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82856.1"
                     /translation="MMHDDDYEGFDDQSENLGNSVQRFEEMLRNHETYFFDADSLIKI
                     TDFYLEKFDYDKAMEVIKYGQNLHPDNYAFKAKEAYITHLQGNSEEALEMIDQLLTVN
                     PKNEDLYLIKGLIYNALGMFQEGRKTFKDLLSFTEFKEDVYFHVAQTYLQTFDYHGAI
                     HYFKNCLFENKKNEVAADELSECFDRAGRASEGITYFQALIDQDPYNALFWFHLGQMY
                     QCCVEYEKAINAYDYCILIDENMDDAYIQSGQCHMLSGNYEKAIQSFKQSFEYNTPEA
                     FVYDSIAQCYFEMGNHEESRSYFKKAVKSNPSFHSSWYGIGMTLSEEERWYEAIHYLK
                     KAIELNGDISDYWFLLGDCERELGNLEEADEAYRKVIEMDPDFLDIWYEYPEFLMEQN
                     RTDEALQYAYLAVKYFPNDIAVHLRYIILQYRNGNIKAAYSLLSAAAKGEDDLREIIE
                     EIYPELLNDSNIQKILEQNN"
     gene            complement(8533..9777)
                     /locus_tag="PKU82_08025"
     CDS             complement(8533..9777)
                     /locus_tag="PKU82_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOZ82857.1"
                     /translation="MAILKPFKGIRPKKELVKRIACRPYDVLDEKEARAETKGDKISF
                     YHVIKPEIDFPDDFDHYAPEIYKKGKENFEAMFKEGVFFQDSKDCYYIYAQSMDGRKQ
                     YGIVGCAAVDDYVNDVIKKHELTRPDKEEDRKNHVRVSGLNYEPVFFAYPQVRKLDEL
                     VEHITSAAPEYDFVTDDHIGHQFWIVDNDSDINIITEEFAKLKNTYVADGHHRTAAAA
                     LVGKERREKNPAHTGKEEYNFFLAVHFPDNQLKIMDYNRVIKDLNGMNSNQFIDKLKE
                     NFVVEEKGSDEYRPAALHEFSMYLEGKWYKLNAKPGTFNDKDPIGVLDVTILSDKVLA
                     PMLNITDLRKDKRIDFVGGLRGLGELKRRVDNGEMKVAFALYPVTMQQLIDIADNNLI
                     MPPKVTWFEPKLRSGLVLHSLD"
     gene            complement(9805..>10695)
                     /locus_tag="PKU82_08030"
     CDS             complement(9805..>10695)
                     /locus_tag="PKU82_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ82858.1"
                     /translation="DKVPQEKLAEALKNFDAILVRSATTVRTDVIDACPNLKVIGRAG
                     VGMDNIDVKYARDKGLAVINTPAASSQSVAELVFAHLFTGVRMLHDANRNMPACATKE
                     TFDKLKKSYSAGIELKGKTIGIIGAGQIGKCVAAIAVGLGMNVLFHRLNNEDVVVDMD
                     FHPALGQKKITLTFKCVSFTKLLIESDFITMHVPFPKGSAPVIGKKELDLMKNGVGIV
                     NTARGGVISEADLIDALNSGKVAFAGIDVFEGEPLINQTLCAHPKVTLSPHIGGSTKE
                     AQERIGIELAEKVVEYFTKK"
BASE COUNT         3199 a         2065 c         1903 g         3528 t
ORIGIN      
        1 ttatcgaatg gtgaataaga aaaacaaaaa tgaccattgg catcgacagt acgatttaaa
       61 tctttcatca caaagtctgc tgcacttttg caaagttgaa atgctttttg atttcctgtt
      121 tgttgccagg ctataaaaag tgcattggta attattccgg tcgcaacaac tgttggctga
      181 taggcaggta tgttggcata acgtgcttcc cagtcaaaat catatcccca acatgcacca
      241 tgaaatcctt ttggaataag tttttcaagc tcatcaataa gtttagcaat attttggttg
      301 agtatatttt tttcttgctg attaacatta atcagttgtg cgtaagcctg aatgcaaagg
      361 ccaagagtaa caggattcag ccctttcgga acaaaaagta gtggtcgtaa attgagtggc
      421 gatcttttaa caagttgttg tgcgccaaag cgaattaact tatttttatt cagcaagggt
      481 aatttaaaaa atggagattt taatgcatcg taagggtcgt atcccttgta gtgatgtgac
      541 tcgatatatt ttcgtaatcg tgatgttgcc gcctcaaggg ttttggatgc atccattgaa
      601 ttaaagaatt attctttctc cattggaggc aatgctttgt aatgctcgga atgttgccag
      661 tgtgctcaaa acagtttctt caaaaggtat tggtgcaggt tttccctgaa caatactatc
      721 tacaaatgct ttaacctcat tctgatgacc tttatcctga ttgcctgtat attttttttc
      781 tgatttaccg aatacagtca gtgttttaaa atcatcaaga actgcgacaa tgccagaacc
      841 gaatacttca agatattctt tgttgatgtt tttattgcca ttgctgaaat aacttaccga
      901 agcggtactg ccattttcaa attgcaggtt aaagcaaacg gtatcgttta gtttttgtgc
      961 atcattcaat gcaactgcac ttatagtggt aagttttgat ccggcaagaa acatacataa
     1021 atcaataaag tgacaggctt caccaacaat tcgtccacct ccaactttta aatcatgcac
     1081 ccaatgatcg gcagggatta cacctgcatt gattctgtaa ttaattgcaa ttggatttcc
     1141 ggaagagaaa gtttttttaa tggtttgaat cattggagca aaccttctgt tgaagccaac
     1201 cattaccctg caattttgct tggtaaattc atgttgtatc tgaaccaatt cttcctctgt
     1261 taagcaaaga ggtttttcaa caaatacatt tttccctgcc tgtaatgcct gcataacata
     1321 acctgcatgt gtttcgtgac gtgttgcaat aaaaactgta ttgatatcac cattgctgaa
     1381 aatgtcctga gcattacctg atgcaaaaga aaattgattt ttgtctgcaa tgtttcgtgc
     1441 attattggga cgtgctgtaa ctataccact gagctttgca ggagtattct ttaaagcagg
     1501 taataaaaaa ttttgtccga aagaacctgc accaatcata cctgcaacca catcactctt
     1561 agtataaaaa gggttggaaa gattgacttt ttgttgcagc ggcttattcg tatcatattt
     1621 caaaaccatt ccaacaaaag gctcggtttt gttcataata agatcgtatg ctgcaggtgc
     1681 ttctttaaaa tcaaatgtat gagtaagcaa atttgaaaga tggattttac cggttgcaat
     1741 taaatcaaca aaagcagcca tgtttctgtt ctctgtccag cgtacatagt cgtaaggata
     1801 gtcaattcct ccttcttcgt attcagtatc gtaacgcccc ggaccataag agcaactcat
     1861 gcgcaggtct aattcttttt taaaatagtt tggtcgttta aaccctgttg gtactgctcc
     1921 cacaataact actttgcctt ttctgcgaca taaggctcct gccagatcaa caggatcgag
     1981 tgagtcagtg ccggcagtga tgattactgc gtcagtgccg tagccgttag taatattgtt
     2041 aattgaactt tcaagatctt cactgttacg ggtaaatgca gcatcaatat cagtatgcaa
     2101 agcaaggtca accatacgtt ggtcaatatc tattccgata gtttttatgc caccggcttt
     2161 aagcatctga acggtaagtt gtccaaccag accaagcccg atgactacac aattttcacc
     2221 taaacgaagg tcggcttgtc ttatcccctg aagtgctatt gaacctaaag ttgtaaagca
     2281 tgcatgttga agggcaattt tatcgtttaa cttaacacaa agatttacag ggacagcaat
     2341 aacttctgca tgaactgcac tgttgccacc acaagctacc aaatcaccaa ctctgaattc
     2401 agtaacatca ggtgcaacag caataatttc gcctgctgtg ctgtaaccta atgatgatgg
     2461 tgcatctaat ttattcatca ccattttata agtgtttaaa atgccgaatg tttttgcagc
     2521 atcaatcact ttttttactt cttcttttct tgctcttgcc ttaccgatat aactcagtcg
     2581 tgcatctttt acggttttgc cttcagtacc tgcactaatc agcgaataat gattgcgtac
     2641 taaaacctgc cctgatgaaa gaaccggaaa cggcacttca agcaactgca tttttccatc
     2701 tttcagattt tgtgttaact gttccatttt ttgcagttta gattgtttat gtggaatgat
     2761 tttgatcgaa ccactttctg aatgctatta atccttcttc taatgttgta gcaggactat
     2821 agttaagtaa ttcttttgct ttacttatgt cggcaaatgt atgaggtaca tcgcctggtt
     2881 gatcaggtaa tttttcaagt aatgcttttt tgcctgcttc tttttctatt gctgcaatca
     2941 gcatattcag attgatagtt ctgttattac caagatttat gatttcgtaa ttgcttttat
     3001 cataatgcat ggctgcaatt ataccattca cgatgtcgcc tacaaaagta tagtcgcggc
     3061 tagtttcacc attgccaaag aatggaattg gtgtaccctg acgtatcagc ttaaaaaatt
     3121 tatgtatagc taagtcaggc cgttgacgag gaccaaacac tgtaaagaag cgcagagata
     3181 taaacctgat accatacaaa tgagcataag tatgcccggt aagttcgcat gagacttttg
     3241 ttgatgcata aggagaaata ggtttcaata caaaatcatt ttcgctccat ggtgtgtttg
     3301 ggttgatgcc atagacacta ctactgcctg caaaaacaaa ctgtttgata tttttttctt
     3361 ttgccttctc caataagttt acagttcctt tgatatttac atcttcataa agcaaaggtt
     3421 tttcaatact cggtctaact ccggcacgtg cagccagatg aactataatg tcgaacggtt
     3481 gttgaaatag tttttcgaga aagtcagtat cacgaatatc accttcatgc aaaaaaaagt
     3541 ttttactctt taatgcttcc gaaatattat ttctttttac agcaggattg taaaaatcat
     3601 caaacacatc aaggcaatgc acttcgtata aatcattagt taaaagagca tcacataaat
     3661 tgctgccaat aaaacctgct ccgcctgtta ctaatacttt tttcaattca ataatattta
     3721 attctcaatt acacttctga atgcatgacc catccgttca accatttttt cttctgtcag
     3781 attttctaaa tagatgcgtc tgctttctgc tcccatttta tttctcaaat ctttatcgtt
     3841 tgccaaaata attatttttt cggctaattg ctgaatattt ttcttttcta ccagataacc
     3901 attttttcca tcatgtacgc tttcggagat tgctgcatgg tctgtagaaa ttacaggcag
     3961 tccggcagcc atagcttcaa cagtaaccca tggatgacct tcattgcgat agtatgtagg
     4021 gaaaacaaaa atatcggctt cgttcaggat gctgaacttt ttatcacctt taacaggccc
     4081 gtgatttatc acaggcagtt caggatgttg ttgtaaaaat tttttaaaat ctgtttttac
     4141 atctgcttca cgccattcac cggcaaatac aaattctacg tttttatttt tttcatatac
     4201 taatggtgct gcgtacaatg cttccaatac tcctttggtg cgcacatagt taccaagaaa
     4261 aagtacttta accttattgt tgtttcgctc attgtagttt accggaaaat ttccaccatt
     4321 gggaataaca aatatttttt cgtcaggtac aatccagtta aatagtttac gcaggttgtt
     4381 gccaagcaca atctgtccgt ttactttttt atgtacaaaa cgaatgaatg atttcatcgt
     4441 ttttccactt tcattatacc agttcagaaa atatccaccg cgaagatgtg taagtacttt
     4501 aatgccaaaa agttttgcaa taagaatata tcctgcatca cgtgtatagc caacattggt
     4561 ttgtcctgca ggtacataaa ccagatcagg gcgatagcgg atacaatacc agaacaactg
     4621 aaaatattgt ttaaaggcta aataaaaatt cgttaaatcg agttttgcta atgtattgat
     4681 gtcacgttta tcagaaaggt caagatgaat cagattaaat tcgtctttaa gttttgagtt
     4741 gagaataatt tcgcaggcaa cagatgggcc aatgtaggga ggaggcagtt tgcccagaaa
     4801 aattacagta tattttttgt cactcatgat gatttaaatt taagataaag agatggaaaa
     4861 aagcgttgca gcaaccaggt tatggttatg ccactgcata acatcagaaa aaaatcaata
     4921 ggtatgcgtg ctctggtgaa tccaatagca attgaatgtg caccggtgaa ggcaagtaaa
     4981 atcaataaca acgtaaatgc ctcaaccgga tgttttttaa acaataatat caatccaaat
     5041 ggaaagagta gtaatatagg cagatatgaa atcaggtaaa cagattttcg tccttcattg
     5101 cttccgaatt tcacgctttt actatcaggg ttacgattcc aactccagag tttttctgct
     5161 tttagccaac ccatagaaac aaattcaggg aaattgtttt ttatccaatt tacagcttct
     5221 tgttgatagg ctgcagatac ttttacggcc tcatcaggtg tgcgtgctgc aagtggatac
     5281 tgtgcatata ttggcaacgg tgggtttaaa cgataaattt cgtctagtga tttgttagct
     5341 tttagatatt caaatgacaa aggattgttg ccttgccaca taccgaatga accatgtgtg
     5401 ctgattaata attttcctgt tagtgaataa tttctaacca accagggtgc cattgccaat
     5461 agacatcctg caacaattaa tgagcaactg ataaatgctg ctgttacatt ttgtgatttg
     5521 aagtagcgat agaatacata gagaaataag ccgggaagaa aagttatgat tgttgctcgt
     5581 gttaaaaatg caagaccgat tataattccg gcaagtacaa gtctttttga tgaaagtttt
     5641 tctgtgagta ataattcaat agtcaataca agtaaaaaaa caaaaagtgt agtgtcgtag
     5701 atagaaaacg aatatgtaaa cagatatgga taaaaagcat gataacatgc tgcaaaaaat
     5761 cctgcaatgg ggtgaaaaag tctggtgccg attttaaata gcagaagaga ggtgatgaga
     5821 tgcaatacag cctgaaggat ggtcagacct aaccagttat atcctgtaat tgcataactg
     5881 atactcatta atgcaggata caaaggttca tagtcaacag aaggaattcc ctgaacaaga
     5941 ctgtattcac cgttaacgta aaggttacat gcaagtgcat taagattggt ttttgaattg
     6001 ccacgattca aaaaggaatc tttaagatga aatccgatag caatcttagc aacgaaccct
     6061 gccaatagaa taataatcag cagttttttt aaatttatat ttgtacttgc agtcaatgaa
     6121 tgtaaaggaa atggaaatat gaaattatac ccttttgggc ggctgcaaaa gtacaacttt
     6181 acaaggcacg attatgcctg aaaagcaata agtttaattg gtaaatctat tgatagattc
     6241 tttatcaaga aaatggaaaa aagtgtcact tcttaaccct aaacgcaacg attcaagagc
     6301 aataacttcg ctgggagcaa tgttgcctaa attcacattg gtgccaaact gcttaatgaa
     6361 ataaacctgt tgcgattttt gtggtgtttc ccagattatt ttatcggcag gcactcttga
     6421 gattatctta ttgataagca ttacgtgtgc ttttccatta ggacgataaa tacccactgt
     6481 accactttcg cgtgcttctg caattacttt ccaaacaccg gcttcaagtt cacgttccat
     6541 catttgaatc caaatgttag gacgaataat aattccttct tcttttgagc ctacttcgga
     6601 aattactgtt acatgtttag caagtttttt aatgtaatcg cattttattt tatgtggaat
     6661 tacgatggag ccatctgata tttcagcata ttccattttg tattttgata atactttcag
     6721 gtacttgtcg aactcatctc tgataagaaa agcttctaac aaagtcccgc caaaataaac
     6781 cggtattccg gcttctttat aaagagcgag ttttttatcc agattcggag taatgagact
     6841 actgccgaaa cctaatttta ccaaatcggt aaattcgcct gaggaggaaa taaaatcttc
     6901 tgtttcacga aggctaaggc ccttatccat caccatagtt aagccattct cacgaggttt
     6961 tgtacttcgt gaaggcattt gactcaattc aaaattcatt gccactaatt gttttgttct
     7021 aaaattttct gaatgttaga gtcattcaaa agttcaggat aaatttcttc aataatttcc
     7081 ctgagatcgt cttcgccttt agcggcagcc gacaacaagc tatatgcagc ttttatgttt
     7141 ccgtttctgt attgtaaaat gatgtatcgc agatgtactg caatgtcatt tggaaaatat
     7201 tttacggcaa gatatgcata ttgcagtgct tcatcagtac ggttttgttc catcaaaaat
     7261 tcgggatatt cataccagat atccaaaaaa tcagggtcca tttcaatcac ttttctataa
     7321 gcttcatcag cttcttctaa gttgcctaat tcacgttcgc aatcaccgag taaaaaccaa
     7381 taatcactta tatcaccgtt tagttcaatt gcttttttaa ggtagtgaat agcttcgtac
     7441 cagcgttctt cttccgacaa tgtcatgcca atgccatacc atgaactgtg aaaagatgga
     7501 ttacttttta cagccttctt aaaataacta cgcgactctt catggtttcc catttcaaaa
     7561 taacactgtg ctatactgtc ataaacaaat gcctcaggtg tgttatattc aaaactttgt
     7621 ttgaaagact gaatcgcttt ttcgtagtta cctgacaaca tgtggcactg cccgctttgt
     7681 atgtaagcat cgtccatgtt ttcgtcaatc agaatacaat agtcgtatgc attgatggct
     7741 ttttcatatt caacacaaca ctgatacatt tgtcccaggt gaaaccagaa caatgcattg
     7801 taagggtcct gatcaattaa tgcctgaaaa taggtgatgc cttctgaagc tcttcctgca
     7861 cggtcgaaac attcgctcag ctcgtcagca gcaacttcgt tttttttgtt ttcgaaaaga
     7921 caatttttaa aataatgtat ggcaccgtga taatcgaatg tctgcaagta tgtttgtgca
     7981 acatgaaagt aaacatcttc tttaaactct gtaaatgaca acaggtcttt gaaagtttta
     8041 cgtccttcct gaaacatacc caatgcatta tatataagac cttttataag ataaaggtct
     8101 tcgttcttgg ggttaacggt aagtagctga tcaatcattt caagcgcctc ttctgagttg
     8161 ccttgtaaat gagtaatgta tgcttctttt gctttaaaag cataattatc ggggtgaagg
     8221 ttctgaccat attttatgac ttccatagcc ttatcatagt caaacttctc taaataaaag
     8281 tcagtaattt ttatcagcga atcggcatca aaaaaatagg tttcatgatt gcgcagcatt
     8341 tcttcaaaac gctgaacgct gttacctaaa ttttcgcttt gatcatcaaa tccttcatag
     8401 tcatcgtcat gcatcataag cttctattgg ttctctcaaa ttgaggtcaa aggtaaaaaa
     8461 tatgaaacaa cctgcagtac ctgtcaggca gacagtcaac agcaggttgt taaatacttg
     8521 actgtaagaa aattagtcta atgaatgcag tacgaggcca ctacgaagtt ttggctcaaa
     8581 ccaggtaact tttggtggca taataagatt attgtcagca atatcaataa gttgctgcat
     8641 tgtaacagga taaagtgcaa aggctacctt catctctcca ttatcaactc ttctctttaa
     8701 ttcaccgaga ccacgaagtc ctccaacaaa atcaattctt ttgtctttgc gaaggtctgt
     8761 gatgtttaac ataggtgcta atactttgtc ggacaaaata gttacgtcca aaacaccaat
     8821 tggatctttg tcgttgaagg tgcccggttt agcatttaat ttataccatt ttccttcaag
     8881 atacatgctg aattcatgta aggcagcagg acgatattcg tcacttcctt tttcttcaac
     8941 aacaaaattt tctttcagtt tgtcaataaa ctgatttgaa ttcataccgt tcaggtcttt
     9001 gatgaccctg ttgtaatcca ttatttttaa ttgattgtcg ggaaaatgca ctgccagaaa
     9061 aaagttgtat tcttctttgc ctgtgtgtgc aggatttttt tcgcgtcttt ctttaccaac
     9121 caatgctgca gcagctgtgc ggtgatgtcc gtcagccaca taagtgtttt taagttttgc
     9181 aaattcttct gtgattatgt ttatgtcgga atcattatcc acaatccaaa actgatgtcc
     9241 gatatgatcg tcagttacaa aatcatattc aggtgcagca gaggtaatgt gttcaaccaa
     9301 ctcatctaat tttctaactt gaggatatgc aaaaaacaca ggctcataat ttaaccctga
     9361 aacacgcacg tgatttttac ggtcttcttc tttatcagga cgtgtaagct catgtttctt
     9421 gataacatca ttcacataat cgtcaacggc tgcacagcca acaatgccat attgtttacg
     9481 gccgtccatg ctttgtgcat aaatgtagta gcagtctttg ctgtcctgaa aaaacacacc
     9541 ttctttaaac atggcttcga aattttcttt gcccttttta taaatttcag gggcataatg
     9601 atcgaagtca tccggaaaat caatttcagg ttttatgaca tgatagaaag aaattttatc
     9661 acctttggtt tctgcacgtg cttctttttc atcaagtaca tcataagggc ggcaagcaat
     9721 ccgctttact aattcttttt ttggtcttat tcctttgaat ggttttaata ttgccatacg
     9781 ttttggtaaa ttttaattct gattttattt tttagtaaaa tattcaacaa ctttttcagc
     9841 aagttcaatg cctatgcgct cctgtgcttc tttggttgaa ccaccaatgt gtggtgacaa
     9901 agttactttg ggatgtgcgc aaagtgtttg gttaattaat ggttcgcctt caaaaacatc
     9961 tattcctgca aaagctactt tccctgagtt gagtgcatca attaaatcgg cttctgaaat
    10021 tacaccacca cgggcagtgt taacaatgcc tacaccattt ttcatcaggt caagttcttt
    10081 cttgccgata accggtgcac ttccttttgg aaaaggcaca tgcatagtta taaaatcact
    10141 ttcaataagg agttttgtaa acgaaacaca tttaaaagtg agtgttattt ttttctgtcc
    10201 taaggcagga tgaaaatcca tatctactac cacatcttcg ttgttcagtc tgtgaaacaa
    10261 aacattcatt cccaaaccaa cggctatggc agctacgcac ttaccaattt gtccggcacc
    10321 gataatgcct attgttttcc cttttaattc tattcctgca gagtaagatt tttttagctt
    10381 atcgaatgtt tctttagtag cgcaggcagg catattgcgg ttggcatcat gcagcatacg
    10441 tactcctgta aataaatgtg caaaaaccaa ttccgcaacc gattgtgatg atgctgccgg
    10501 ggtattgatt actgcaagtc ctttgtcgcg ggcatacttt acatcaatat tatccatacc
    10561 tacaccggca cgaccaatta ctttaaggtt tggacatgca tcaataacat ctgtgcgaac
    10621 agttgtagca cttcttacta atatagcatc aaaattcttt aatgcttccg caagtttttc
    10681 ctgaggtact ttatc
//
LOCUS       DAOOOL010000087        10416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_590_length_10416_cov_86.146704, whole genome shotgun sequence.
ACCESSION   DAOOOL010000087 DAOOOL010000000
VERSION     DAOOOL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_590_length_10416_cov_86.146704"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PKU82_08035"
     CDS             <1..1346
                     /locus_tag="PKU82_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOZ82859.1"
                     /translation="THESKLYGLTIFVSIVTFQIVYVFVQWLIKHRRDYLFYIAYLFF
                     VIVFGLSHYDKYLNFYPFSGIAERYYEYINLSAPLLCILLYFRFMREFLNLKHRRPDI
                     NTMVKNLEFFLIAYTLLSPFFIWLSLDIAVWKMIFNVVAIITITAGLFIIINFLKKSI
                     PLDRFAMTGASLIAVGSIFDIALSFLEFRGFNIPFDIHLPLMVCVIGELTTFTLGLAF
                     KTQFVENEKLFVEKNFLSEKSEKQQLQLDILKLRNNLARELHDDIGAQLSLARMLMSK
                     ARADGNERVVDQSMQLLEESINKLRSVTNELENTTLQNEGFQKATEKLLSTISEFQPI
                     HCNFYAKGVEKRLHLDVEYHLFRITQELINNTLKYAHASIISIIVLGDRDKFYFIYED
                     NGLGFDLKNSGRGKGLNNILQRVKELGTKTEFIAQPGGGMRCEFKIDYEQSMQIA"
     gene            1356..2021
                     /locus_tag="PKU82_08040"
     CDS             1356..2021
                     /locus_tag="PKU82_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82860.1"
                     /translation="MALSNLKILIADDHKLFAEGLTYLLKQELPIESIDTVYNGKSAI
                     GKVLNTDYDIIIMDVNMPLLNGIETCAAIKRFRTQTKIVFVSMKADMLTVAQALKAGA
                     DAYILKGNDHNDFITAFKQINNNEIFLSEQIKHYFQDDNPNQIRKNAIACVDYSNSLI
                     SVREKEIIKLVCDGLTSDKIAEVLSISTRTVDTHRNNILNKLKLNNIASLVRFALENK
                     LVD"
     gene            2308..2928
                     /locus_tag="PKU82_08045"
     CDS             2308..2928
                     /locus_tag="PKU82_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82861.1"
                     /translation="MKKLIIVLSVAATAAIIGCSKDDLTSPQALQNKNTTTATNDVSA
                     TDERLINSGTVLVPAPNQGYVKVFLFGAAKLSADKPETFVDINKRDVSQRAFDASFKA
                     MYVKFNERWIYVPGIIPNYGIEGKLTYRVSSSQASDPDATNQILAWHRFTLTIRNLNN
                     RPYTGRLVSLSNIKVLIIPAKSEPGGHDPDLRNYEATMTHFGLPLD"
     gene            3110..4546
                     /locus_tag="PKU82_08050"
     CDS             3110..4546
                     /locus_tag="PKU82_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOZ82862.1"
                     /translation="MTENENFQKIKHLYNRAGFGLHYSELQKLSRTKLNKLVADMIEK
                     AKTDVPITTIAPGHVKSVMLKNAGLTKKEGLSPEQLKERQKLLVSLNKQLKNLNTEWF
                     FRMMDIDSPLREKMTLFWNNHFACREEGNPYFAQVLNNIQRKHALGDFRQLLLEVSQS
                     ASMLNFLNNQQNKKGRPNENFARELMELFTLGRGNYSEKDIKESARAFTGWVHDAEGN
                     FVFNTKNHDNGIKTFFGKEGNFNGEDIIDMILQKPEAAVFIARKAYRFFVNDVPDETH
                     VQELGNHFYKSKYDISVLMNKMFTADWFYAPENTGNKIKSPVEFIVGLSRQFNVTYKR
                     QEIILQLQRGLGQTLFYPPNVAGWAGGRNWIDSSSLMLRLTIPSQILNDGSLNFSAKT
                     DPDDENIVAMKQMDDAVIEAKKKGSRIEINWENFWADFPKDYSVQQIAGFILQCKPSA
                     QLNQIISSSIDKKNAIIKTASSPEYQLC"
     gene            4569..5768
                     /locus_tag="PKU82_08055"
     CDS             4569..5768
                     /locus_tag="PKU82_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOZ82863.1"
                     /translation="MKRRDFLKQSVFASAGSMLIPAFLKPFELIAKSSLSGHKNLVII
                     QLSGGNDGLNTIVPYGNDVYYQLRNTIAIPQSEIIKLNDMQGLNPTLAPLKEIYDQGW
                     MSIINNVGYPNPNRSHFRSMDIWQTASDSNQFLSTGWIGRYLDSNCQMCKYPYNAIEV
                     DDTLSLTLKGDSKKGIALQDPDKLFRTTNTPFFGKLIEHDKEHLDEDNLGYLYKTMIE
                     TYSSASYIKETTKNYDNNFVYPESSFAKKLKTVARFILSGLDTRVYYVSLSGFDTHVN
                     QMAQQKKLFTQFSEGMNAFVKELNNNKKLEDTLVIAFSEFGRRVAQNASRGTDHGTAN
                     VMMLFGGQLKKQGIYNDAPDLNDLDAGDLKYKVDFREIYATVLNKWLNVDNKLILPDS
                     TGTMDFI"
     gene            complement(5786..7180)
                     /locus_tag="PKU82_08060"
     CDS             complement(5786..7180)
                     /locus_tag="PKU82_08060"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOZ82864.1"
                     /translation="MKNKYSYVLNSIFKIIVILLLPNSINAQFGSPFVIDSANNTLVN
                     TINIVDLDNDNQKDILVSNLYDSVYWYKNNGLLFNRMSAISNTVKRPYFVDCADVDGN
                     GLQDVLVSYDDVYAGLKLFKNFGGNTWQQIIINDSIDAFVVRSYLVDLDNDSDLDIIS
                     CQDLEVAIYEQSAGTFSGMIKVADSSEFYNLVVHDFNGDGFVDFSVHSAAGFRLFINN
                     HNLTFSPQTFTTALYLTLESCDIDNDGDFDLFYPDSAVSFNLITLKNDGSGGFSNFQS
                     VMYHAGNNQNPPLKFLTLDNDTFPDALYIPLVNRGLYYKSNDGVGGLNFSHFIDSVYR
                     YTFVNGGDLDGDNDNDLVWFANEPFGIKRVGYTLNNSGNTDYNELSSQEFYFYPNPVS
                     NNILNIKYFSPTSSGGVFEIVDAIGKTVFKYTLPLYSSNHQITLPQLQNGVYNAMLTY
                     EGAKICRKLAIVDN"
     gene            complement(7358..8332)
                     /locus_tag="PKU82_08065"
     CDS             complement(7358..8332)
                     /locus_tag="PKU82_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOZ82865.1"
                     /translation="MESIDFLQREIERELKLLRFGHEPANLYEPMEYIVELGGKRMRP
                     LLVLIGAGLFETNYQKAMKAAIGIELFHNFTLLHDDIMDKATLRRNKPTVHEKWSADI
                     AILSGDALFVRASEYIMQVDDAILRPVMQLFYKTAMQVCEGQQYDMDFGRLREITPED
                     YIGMITLKTAVLPACCLATGAIIANASEEDKLRLYEFGKNIGIAFQIQDDLLDAFGDD
                     TKFGKRPGGDIIEGKKTFLFSEACRLLNGKEHLDFLTLYNSHNLSNHEKITEVMQVFN
                     NLELHHHTMRTIQSYYQKGLEQLAGLNVQAKNVLELKLFCEKLLIREH"
     gene            complement(8342..9286)
                     /locus_tag="PKU82_08070"
     CDS             complement(8342..9286)
                     /locus_tag="PKU82_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ82866.1"
                     /translation="MKSKILVTGGAGFVGSALAEKLADNADNFVVVVDNLLTGSMRKV
                     PASKHNNVKFIKADVNDFSDISSIFYAYAFDYVFHYAAVVGVKRTLDHPSWVLKDLTG
                     LKNILDLSKNTGVKRVYFSSSSEVYGEPVEYPQNETTTPLNSRLPYAIVKNLGEAYLR
                     AYKQEFDLDFTIFRFFNTYGPKQSTDFVMSKFIEAALSDKDITIYGDGSQTRTFCYVD
                     DNVEACLNAFYTHRFVNDVINVGNDHETSILELAQLVKKILNSKSNIVHLPALKEGDM
                     TRRCPDIGKMKILLNRELTPLETGMERVIEVFRNKKKK"
     gene            complement(9301..10170)
                     /gene="rfbA"
                     /locus_tag="PKU82_08075"
     CDS             complement(9301..10170)
                     /gene="rfbA"
                     /locus_tag="PKU82_08075"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOZ82867.1"
                     /translation="MKGIVLAGGSGTRLHPLTLAMSKQLMPVYDKPMIYYPLSVLMMA
                     GIREILIISTPHDLPHFKKLLGSGEQIGCRFEYAEQAVPNGLAQAFVIGEKFIGSDSA
                     ALVLGDNIFYGQGLGRILADNNKINGGLVFAYHVSDPERYGVVEFDGNYKALSIEEKP
                     QKPKSNFAVPGLYFYDNTVIEVAKNLKPSARGEYEITDVNKHYLQQGKLKVQLLERGT
                     AWLDTGTFNSLLQAGNFVNVIEERQGLKIGCIEEIAYRMKFIDKEQLRKVAEPLTKSG
                     YGNYLMKIIDQNL"
BASE COUNT         3467 a         1882 c         1823 g         3244 t
ORIGIN      
        1 acacacacga aagtaaatta tatggattaa ccatctttgt ttctatagtt acgttccaga
       61 ttgtttatgt ttttgtgcaa tggcttatca aacaccgaag ggattatctg ttttatattg
      121 cctatttgtt ttttgtcatt gtttttggat taagccacta cgacaagtat ttaaactttt
      181 atcctttttc aggaattgca gagcgatatt atgagtatat aaacctatca gcaccgctgc
      241 tttgcattct tctttacttc agatttatgc gtgagttttt gaacttaaaa cacaggcgac
      301 ctgatattaa tacaatggta aagaaccttg agttttttct gattgcttac acactactta
      361 gcccgttttt tatctggctt tcacttgata ttgctgtctg gaaaatgatc tttaatgttg
      421 ttgcaataat tacaatcact gcgggattat ttataataat taacttttta aagaagagta
      481 ttccactcga cagatttgct atgacaggtg cgtcattaat tgctgtgggt tcaatatttg
      541 atattgcact tagctttctt gagttcagag gatttaatat cccctttgac atacacctgc
      601 cattgatggt ttgtgttatt ggggaactca caacttttac tctgggactt gcatttaaaa
      661 cacagtttgt cgaaaatgaa aaactatttg tagaaaaaaa ctttctttcc gaaaaaagtg
      721 aaaaacaaca gttacagtta gacattttaa agcttagaaa taatctggca cgcgaactcc
      781 acgatgatat tggtgcacaa ctatcacttg ccagaatgtt gatgagtaag gcacgtgctg
      841 atggtaatga aagggtagta gatcaatcta tgcaactgct cgaagagtca ataaacaaac
      901 tgcgttctgt tactaacgaa ttagaaaata ctactttaca gaatgaaggt tttcagaaag
      961 caacagaaaa attgttgagc actatttcgg agtttcagcc tattcattgt aacttctatg
     1021 caaaaggtgt tgaaaaacga ttgcatcttg atgttgaata tcatttattc cgcattactc
     1081 aagagctaat caataacact ctaaaatatg cccatgcttc tattataagc atcattgttt
     1141 taggcgatag ggataagttt tattttatct atgaagacaa tggtttgggc tttgatttaa
     1201 aaaactcagg tcgtggcaag ggcttaaata atattttgca gcgcgtaaaa gaattaggca
     1261 ccaagacaga gtttattgct caacccggtg gtggcatgcg ctgtgaattc aaaattgact
     1321 atgaacaatc aatgcaaata gcctgattat ttaatatggc actaagtaac ttaaaaattt
     1381 tgattgctga tgaccataag ctttttgccg aaggcctcac ctatctttta aagcaggagt
     1441 taccaataga aagtattgac acggtttata acgggaaatc agccattgga aaagtgttga
     1501 atacagatta tgatattatt attatggatg ttaatatgcc tttattaaac ggcatagaaa
     1561 cctgtgcagc gataaaaaga ttcagaacac aaaccaaaat agtttttgtg agcatgaaag
     1621 ccgatatgct aacagttgca caggcattaa aagcaggtgc cgatgcatac atattaaaag
     1681 gtaacgatca taatgatttt ataactgcat ttaaacagat taacaacaat gagatttttc
     1741 taagtgaaca aattaaacat tactttcagg atgataatcc taatcaaatc cgtaaaaatg
     1801 caattgcctg tgtagattat tccaacagtc tgatttctgt tcgcgaaaaa gaaattatta
     1861 aattagtttg tgatggatta acgagtgata aaattgctga agtactttct atctcaacca
     1921 gaacggttga tacacacaga aacaatatac tcaataagct aaaactaaac aacattgcct
     1981 cattggttcg ctttgcctta gagaataagc tggtggatta atttcttctg caagaaataa
     2041 aatcagcact taattctaat aattaaaaat tacgaagttt tacgtattgc attcagtagt
     2101 ttgcgggctt acttttacac tatcaatttc acaacaacaa atacaaaggg agttagaagt
     2161 tcaaatacaa atcatcaaaa taaacaacat gatataattt ttacactgat ttataaaaca
     2221 acaaaagttg aattaattaa ggggacagtt gttctccaac aaaaatagtt aaccaacaaa
     2281 atgtctaaca gttaaaaatt agtcaagatg aaaaagctga ttattgtatt aagtgtagca
     2341 gcaaccgcgg caattatcgg ttgcagcaag gacgatctca ccagcccaca ggctctgcaa
     2401 aacaaaaaca ccacaacagc aacaaatgat gttagtgcaa cagatgaacg cctcatcaat
     2461 tccggcactg tattggtgcc tgcgcccaac caaggttatg tcaaagtgtt tctttttggt
     2521 gcggcaaaac tctctgccga caaacccgaa acatttgttg acatcaacaa aagagatgta
     2581 tcgcaacgag cattcgatgc cagcttcaaa gctatgtatg taaaattcaa tgagcgttgg
     2641 atatatgttc caggaataat tccaaactat ggcatagaag gtaaactaac ttatagagtt
     2701 tcatcatcac aagccagcga ccctgacgct acaaaccaaa ttcttgcatg gcatcggttt
     2761 acactcacca ttcgcaactt aaacaacaga ccatatacag gcaggttggt atctttaagc
     2821 aacataaaag ttttgataat acctgcaaag agcgaacccg gtggtcatga ccctgattta
     2881 agaaactatg aagccaccat gacccatttt ggcttaccgc ttgattaatg ttgaagtttt
     2941 ctaaacaaag gaggcggctg cattcgcagc cgcctcgttt gttttattgc tattggctca
     3001 cttagaaatt atatccgtcc gattttatcc tattatcatt cttaccttta tttttgttca
     3061 gactaaacct tctttacaat atcgtgtcaa atacacagtt tgttttgtta tgactgaaaa
     3121 cgaaaacttt caaaaaataa agcaccttta taaccgtgcc ggttttggat tacactattc
     3181 tgagctgcag aaactaagcc gcacaaagct gaataagtta gttgccgata tgattgaaaa
     3241 agcaaagacc gatgtaccaa ttacaaccat tgctcccggc catgtaaaat ctgtgatgct
     3301 taaaaatgca ggacttacca agaaagaagg cttgtcaccg gagcaactaa aagaaaggca
     3361 aaaactttta gtgagcctta acaaacaact taaaaacctg aatacagaat ggttcttccg
     3421 catgatggat attgacagcc cactacgcga aaaaatgacc ttattctgga acaatcactt
     3481 tgcatgtcgc gaagaaggga atccatattt tgcacaagtg ctcaataaca ttcagcgcaa
     3541 acatgcatta ggtgatttca ggcagttgtt attggaagta tctcagtcgg catctatgtt
     3601 aaatttttta aataatcagc aaaataaaaa aggcagacct aatgaaaact ttgcacgcga
     3661 actgatggag ttgtttacac ttggtcgtgg taactattca gaaaaagata ttaaagaatc
     3721 agcacgtgca tttactggtt gggtgcacga tgcagaagga aattttgtgt tcaacaccaa
     3781 gaatcacgac aatggcataa aaacattttt cggaaaagaa ggcaacttca acggtgaaga
     3841 tattattgat atgattttgc aaaagcctga ggctgctgta tttattgcaa gaaaggctta
     3901 tcgctttttt gttaacgatg tgcctgatga aacacatgtg caggaattgg gtaaccattt
     3961 ttataaaagc aagtacgata tttctgtact gatgaataaa atgtttactg ccgattggtt
     4021 ttatgctcct gaaaacacag gtaataaaat taaatctccg gtagagttta tagttgggct
     4081 tagtcgtcag tttaatgtta cttacaaaag gcaggaaatt attttacaac tgcaaagagg
     4141 tttggggcaa acattatttt atcctcctaa tgttgcaggt tgggcaggcg gtcgaaactg
     4201 gattgacagc tcatcattga tgttgcgatt aacaattcct tctcagattc ttaatgatgg
     4261 aagtttaaat ttttctgcta aaaccgatcc tgacgatgaa aacattgtgg caatgaaaca
     4321 aatggatgat gctgttatag aagcaaaaaa gaaaggttca agaatagaaa ttaactggga
     4381 aaatttttgg gcagattttc caaaagatta ttcagtgcag caaatagcag gttttattct
     4441 gcagtgtaag ccgagtgcac agttgaatca aatcatcagt agttctatag ataagaagaa
     4501 tgcaattata aaaacggcaa gctctccgga gtatcaatta tgctgaagca aaaaaataaa
     4561 aagcaacgat gaaaagaaga gattttctaa aacaatcagt ttttgcaagt gcaggcagta
     4621 tgttgatacc tgctttttta aaaccatttg aactgattgc aaaaagtagt ttaagtggac
     4681 ataaaaatct tgtcatcatt caactttccg gtggcaatga cggactgaac actattgtgc
     4741 cttatggtaa tgatgtatat tatcagttgc gaaacactat tgccattcca caatcggaaa
     4801 tcattaaatt aaatgatatg cagggattga atcctacatt agctccacta aaagaaattt
     4861 acgatcaggg ttggatgagt atcatcaata atgtgggtta tccgaatcca aaccgctctc
     4921 actttaggtc aatggacatc tggcaaacag caagtgattc caatcagttt ctttctacag
     4981 gatggattgg acgctattta gattcaaact gtcagatgtg caagtatcca tacaatgcta
     5041 ttgaagtgga cgacacctta tctttaactt tgaaaggtga ttctaaaaaa ggtattgcac
     5101 ttcaggatcc ggacaaattg tttcgtacca ccaacacacc tttttttggc aagttgatag
     5161 aacacgataa agagcatctt gacgaagata atctaggata cttgtacaaa accatgattg
     5221 aaacatattc atcggcaagc tatattaaag aaacaacaaa gaattatgat aataactttg
     5281 tgtatcccga atcgtcattt gcaaagaaat taaaaaccgt tgcgcgattt atattatcag
     5341 gacttgatac aagagtttat tatgtttctc tcagtggatt tgatacgcat gtaaaccaaa
     5401 tggcgcaaca aaaaaaattg tttactcagt tttctgaagg catgaatgca tttgtaaaag
     5461 agttgaacaa taataaaaaa cttgaagata cattagtgat tgccttttcg gaatttggta
     5521 gaagagtggc gcaaaatgca agccgtggca ccgaccatgg tacagcaaat gttatgatgc
     5581 tgtttggcgg tcagttaaag aagcagggaa tatataatga tgctcctgat ttgaatgatc
     5641 ttgatgcagg agatttaaaa tataaagttg atttcagaga gatatatgca acagtattga
     5701 acaaatggct caatgtggac aataaattaa ttcttcctga ttcaacaggt acaatggatt
     5761 ttatttgacg caagatacaa tgagattagt tatcaactat agctaatttc ctgcaaattt
     5821 ttgctccttc ataagttaac atagcattgt aaactccatt ttgtaattgt ggtaatgtga
     5881 tttgatgatt gctgctatac aatggcaatg tatatttaaa aacagttttt ccaattgcat
     5941 caacaatttc aaaaacacca cctgaagaag ttggtgaaaa atatttaatg tttagaatat
     6001 tattacttac aggattggga taaaaataaa attcctgaga tgacaactca ttataatcag
     6061 tgttgccgga attgttcagg gtatatccta ctcgtttaat gccgaagggt tcattggcaa
     6121 accatacaag gtcattatca ttatcgccat caagatcacc accattaaca aaagtataac
     6181 gataaacaga atcaataaaa tgagagaagt ttaatccacc tactccatca tttgacttgt
     6241 aatagaggcc tctgtttaca agtggaatat aaagtgcatc cggaaatgta tcattatcta
     6301 aagtgagaaa ttttaatggt ggattttgat tgttacctgc atggtacatc acggattgaa
     6361 aattactaaa acctcccgag ccatcatttt tcaaagttat caagttgaaa gaaacagctg
     6421 aatcgggata gaaaagatca aagtcaccat cattatcaat gtcacaagat tctaaagtga
     6481 gataaagtgc agttgtaaat gtttgaggac tgaaagtaag gttatgatta tttataaaaa
     6541 gcctgaatcc agcagcagaa tgaactgaaa aatcaacaaa gccatcacca ttaaaatcat
     6601 gcacaactaa attgtaaaat tcactactgt ctgctacttt aatcattcct gaaaaagttc
     6661 ctgcagattg ctcataaatt gcaacctcta aatcctgaca actgataatg tcaagatctg
     6721 aatcgttatc taagtcaact aaataacttc ttactacgaa agcatcaata ctatcattga
     6781 tgattatttg ctgccaggtg tttccaccga aatttttaaa taacttcaat cctgcataga
     6841 catcatcata actcaccaaa acatcttgta atccgttacc atcaacatct gcacaatcta
     6901 caaaatatgg acgtttcact gtgtttgaaa ttgcagacat acgattaaat aaaagtccat
     6961 tgtttttata ccaataaaca gaatcatata agtttgatac taaaatatcc ttttggttat
     7021 cattatcaag gtcaacaata ttgattgtat tgactaaagt gttgttggct gaatcaataa
     7081 cgaatggtga accaaattga gcattgatag aatttggtaa taacagaata acaatgatct
     7141 tgaaaataga attcaacaca taagagtatt tgtttttcat actgtgacgt tctaatcaga
     7201 tactaaaact gtttcaaatt gaagtgccct ttatagaatg tgtaaaaata aatttttcct
     7261 tatacgaact tcataaggat tgtgtaaaat agttgtgatt cacaaaaacg taaatgtaag
     7321 gtttaaatta atttccagaa aaggttatta cacagtttca atgctccctt atcaacagct
     7381 tctcacaaaa taattttaat tcaagaacat tttttgcctg cacatttaag cccgcaagtt
     7441 gctccaaccc tttttgataa taggattgaa ttgtccgcat ggtatgatga tgcaattcta
     7501 agttattgaa cacctgcatc acttcagtaa ttttttcgtg attggataaa ttgtgtgagt
     7561 tatacagtgt cagaaaatca agatgctctt tgccgtttaa cagcctgcat gcttctgaaa
     7621 aaagaaatgt ttttttaccc tcaataatat cgccaccggg acgtttacca aatttagtat
     7681 catcaccaaa agcatctaac aaatcatcct gaatctgaaa tgcaatacct atattttttc
     7741 caaactcata caatcgtaat ttgtcttctt ctgaggcatt ggcaattatt gcacctgttg
     7801 ccagacaaca tgcaggtagc accgctgttt ttaaagtaat catgccaata taatcttcag
     7861 gagtaatttc gcgcaatcgc ccaaagtcca tgtcgtactg ttgtccttcg caaacctgca
     7921 tagctgtttt ataaaacaac tgcatcaccg gtcttagtat ggcatcatca acctgcataa
     7981 tgtattcact ggctcttaca aaaagcgcat caccggaaag tattgcaata tcggcactcc
     8041 atttttcatg caccgttggt ttgtttcttc tcaaagttgc cttatccatg atgtcatcat
     8101 gcagcagtgt aaaattgtga aacaactcaa tgcctatggc agctttcatt gctttctgat
     8161 aatttgtttc aaacaagcct gctcctataa gaaccaataa tggccgcatg cgtttgccac
     8221 caagctcaac tatatattcc ataggctcat atagattggc cggctcatga ccaaagcgca
     8281 gcaactttaa ttcgcgttca atttcgcgtt gcagaaagtc aatactttcc atcaaaaaaa
     8341 attatttttt ctttttattt ctgaaaacct caatcactct ttccatgcct gtttcaagag
     8401 gcgttaactc gcgattgagt aatattttca ttttgccaat atcaggacat ctgcgtgtca
     8461 tatcaccttc ttttaaagca ggtaaatgca caatgttcga ttttgaattc agaattttct
     8521 ttaccaactg cgccaattcc agaatagaag tttcatggtc attgcccaca ttgatgacgt
     8581 cattcacaaa acgatgtgtg tagaaggcat ttaaacaggc ttcaacatta tcatcaacat
     8641 aacagaaggt tcgtgtttgt gaaccatcgc catagatagt aatgtcttta tcgctcaatg
     8701 cagcttcaat aaatttcgac attacaaagt ctgtactctg cttaggtccg tatgtgttga
     8761 agaatcggaa aatggtaaag tcaagatcaa actcctgctt gtaggcacgc agataggctt
     8821 cgccaagatt ttttacaatg gcataaggaa gccttgagtt taaaggtgtt gttgtttcat
     8881 tttgtggata ctcaacaggc tcaccataaa cttcgctgct gctgctgaag taaacacgtt
     8941 ttactcctgt gttctttgat aagtcaagaa tatttttcaa tcctgtaaga tcttttaata
     9001 cccatgatgg atgatcgagt gtgcgtttta caccaactac agcagcataa tgaaaaacat
     9061 agtcaaatgc ataggcataa aaaatggaag atatatcgct gaaatcattt acatctgctt
     9121 taataaattt aacattgtta tgtttcgatg ccggaacctt tcgcatggaa cctgtaagca
     9181 gattatcaac tacaacaaca aaattgtcag cattgtccgc taatttttct gccagggcac
     9241 ttcctacaaa tcctgctcca ccggttacta aaattttact tttcatatat tttgtttcgc
     9301 ttataaattt tgatctatta ttttcatcag ataatttcca tagccgcttt ttgttaatgg
     9361 ctctgctact ttccgcaatt gttctttatc aataaatttc atacggtagg ctatttcttc
     9421 tatacaacct atttttaaac cttgtctttc ttcaattaca ttcacaaagt ttcctgcttg
     9481 cagtaatgag ttaaacgtac ctgtatcgag ccaggctgta ccacgctcaa gtagctgcac
     9541 ttttaattta ccttgttgca gatagtgctt gtttacatcg gtaatctcat attcaccacg
     9601 tgcacttggc tttaaatttt ttgcaacttc aataacggtg ttgtcataaa aatacaatcc
     9661 gggaacagca aagttcgatt tgggtttttg aggtttctct tctatggaaa gtgctttata
     9721 gttgccatca aactcaacta caccatatct ttcagggtct gaaacgtgat aggcaaaaac
     9781 caatcctccg tttattttat tgttgtctgc aagaatacgt cctaatccct gaccataaaa
     9841 aatattatcg ccaagaacca aagctgcact gtcgcttcca ataaattttt caccgataac
     9901 aaatgcttgt gccagtccgt taggaacagc ctgttctgca tattcaaaac ggcaaccaat
     9961 ttgttcgcca ctgcctaaaa gttttttaaa gtgaggcaag tcatgcggtg tgcttatgat
    10021 taaaatttct cttattccgg ccatcatcaa aacagatagc ggataataaa tcataggttt
    10081 atcatacacc ggcataagct gtttgctcat ggctaatgta agtgggtgca gacgtgtgcc
    10141 gctacctccg gctaaaacaa ttcccttcat cgtatggttg caaaagtgta tttgtacgta
    10201 aaattacgtc aggtggtttc taaaaattta aggctgtaaa ggtaataatg ttataaaata
    10261 acagaaatgt caatttgttg aggtctatga gcctgttttg aagaaaccct gttttgaaaa
    10321 aataattaca tcacatagaa ttaaaataag gttttgctgt tggccctctt accaaactac
    10381 aaattttaag taaaaatgta ccacagtttc gtattt
//
LOCUS       DAOOOL010000088        10355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_593_length_10355_cov_67.060485, whole genome shotgun sequence.
ACCESSION   DAOOOL010000088 DAOOOL010000000
VERSION     DAOOOL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_593_length_10355_cov_67.060485"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKU82_08080"
     CDS             complement(<1..769)
                     /locus_tag="PKU82_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82868.1"
                     /translation="MKTESLKISLNKLIPVFMMLAGIYFGVMPYFGWDMGRIPGDLGD
                     ARFNYYILEHGYQYLTDQIHGFWDASSMYPYKNVIALSDNLIGTMPIYAIFRVFNVDR
                     ETSFQLWIIVLFLLNYWSCFYVLKKFGINNLIAASCAFIYGFGLYNHGQIFHIQVFPR
                     FIAPLVIYWSIRFIQTLKSRYFTFLLLGLAYQFYCGIYLGFFLAYFIMFLFIAAFIVN
                     LKLPFELNKKALLNYTLSIIGTIIVLLPLFLPYIHSAD"
     gene            complement(800..1516)
                     /locus_tag="PKU82_08085"
     CDS             complement(800..1516)
                     /locus_tag="PKU82_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ82869.1"
                     /translation="MIQKLSIIIPAYNEARTIHLILDKVLAVKLTNNISKEIILVNDC
                     SKDTTKEVIEKYITDHPEADMRLFNQPVNMGKGAALHKGIELATGEYLIIQDADLEYD
                     PEEYNILLKPILTGFADVVYGSRFLGGKPHRILFFWHSIGNKFLTFCSNMFTNLNLTD
                     METCYKMFNTKMVQSLKLKEKRFGFEPEVTAKIARIPNVRIYEVGISYYGRTYAEGKK
                     IGWKDGFRALWCIVRYGLSH"
     gene            complement(1928..2077)
                     /locus_tag="PKU82_08090"
     CDS             complement(1928..2077)
                     /locus_tag="PKU82_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOZ82870.1"
                     /translation="MELLSDLFRFIIQRKKYWLAPLIIVILLMSVLIIAGSNPAFAPF
                     IYTLF"
     gene            2190..3785
                     /locus_tag="PKU82_08095"
     CDS             2190..3785
                     /locus_tag="PKU82_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOZ82871.1"
                     /translation="MLLKQEIEKRRTFAIISHPDAGKTTLTEKLLLFGGAIQVAGAVK
                     SNKIKKTAASDFMEIEKQRGISVATSVMTFFYKNCQINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVKGVEPQTEKLMEVCRMRNTPVIIFINKMDREGQNPFDLLDELEEKLF
                     IHTRPLSWPISSGSTFQGVYNLHNKSLMLFRANQKASDSDVLPIKDMQDPLLDKQLGR
                     YVDQLREDVELIEGVYEPFNKQLYSEGLLAPVFFGSGINNFGVKELLDTFIEIAPEPK
                     SRQTDQRTVTPEESKFSGFVFKIHANIDPRHRDRIAFLRVCSGMFERGKFYHHVRLNK
                     DLRFNNPATFMAQEKSIVEQAFPGDVIGLYDTGNFKIGDTLTEGEELMFKGIPSFSPE
                     IFKELINTDPMKTKQLEKGIEQLTDEGVAQLFIQNGGNRKIIGTVGELQFEVIQYRLQ
                     NEYGASCKFITQNISKACWLTSKDKNKLDEFMRFKMNNIAFDKDQNPVYLADSQWMLD
                     QMIKNYPEVQFHFTSEFKMEVAV"
     gene            complement(3782..4357)
                     /locus_tag="PKU82_08100"
     CDS             complement(3782..4357)
                     /locus_tag="PKU82_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82872.1"
                     /translation="MFSSEEIQHAISADFFNIKRAITDKVYSNFAELGTMAKEKLESD
                     FKIESTIINRSKIYRGENLEGLPWINCDAPGWFNKEETFAIRFLFWWANHYSVTLHLS
                     GKFLNMLDYDKMQSSKSENVLLCINEKPWRYDLHKDNYIPLAEIKDVKPRCRKMNFIK
                     LSIAYPIATLHDFNSQNKKALMCLIGLLRQN"
     gene            4417..4980
                     /locus_tag="PKU82_08105"
     CDS             4417..4980
                     /locus_tag="PKU82_08105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOZ82873.1"
                     /translation="MKLTIISASVREGRKSHRAALFFKNFLIKKYQADVSIADLNELN
                     FPIFEERLRFQKNKSPEVVQFAENIKQSDGVLIITPEYNGGYPASLKNVIDLMYDEWR
                     RKPIAIATVSDGMFGGMQVITSLQFILWKIRAWTVPAMFPMPQIDKNFDVDGTPVDVA
                     GTEKRATVFCDEWMWCIEANRRMQLNH"
     gene            5044..5676
                     /gene="msrA"
                     /locus_tag="PKU82_08110"
     CDS             5044..5676
                     /gene="msrA"
                     /locus_tag="PKU82_08110"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOZ82874.1"
                     /translation="MTSLIAIILLTGCQNQAQSLREISNSTNMQSNIATDTATFGTGC
                     FWCTEAVFESLEGVISATSGYSGGNVADPTYEQVCSGNTGHAECIQVVYDSKKISYDD
                     LLQVFWESHDPTTLNKQGADVGTQYRSVIFYHNPQQKQLAESYKQKLNNENTFGKPVV
                     TEITAATTFYPAENYHQEYFKLNGHAPYCQFVIQPKVEKIRKIFSSKIKK"
     gene            complement(5683..6570)
                     /locus_tag="PKU82_08115"
     CDS             complement(5683..6570)
                     /locus_tag="PKU82_08115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOZ82875.1"
                     /translation="MEHFRESLLILISTPIYIIVIGAEIFFSAFHHLQLYSKKGTLVN
                     LYLMLLNMGLDIIMRTASLLILNYFFDFRIINSYPTAWIYWLALFLAEDFMYYWLHRI
                     DHFCRLFWAVHVTHHSSEEFNLTVGFRSSVLQPLYRFIYFIPLSIVGFKAIDIMFIYS
                     ATQIFGILVHTQTVGKLGWLEYFLVTPSHHRVHHASNVCYLDRNMGMVLIIWDKLFGT
                     FQRELENEPIKYGLTNNLHSNNPGKIVFHEWQAIAHDLKKKTSVKNKIMYLLGPPGWS
                     HDGDCKTSAQLRKALLKKK"
     gene            complement(6592..7845)
                     /locus_tag="PKU82_08120"
     CDS             complement(6592..7845)
                     /locus_tag="PKU82_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOZ82876.1"
                     /translation="MPSVCFYFQVHQPFRIKHYSVFDIGKDHFYEDGEKNKWIMDKVS
                     EKCYLPANKKMLELIKRHKGKFRISYSLSGTVIEQMELFRPDVLQSFVDLSKTGCVEF
                     LSETYFHSLSFLYNKEEFERQIKEHDQKIEHYFKQKPTVFRNTELIYNNELAAFIEKM
                     GFKGILCEGVDRLLKDRHPNHLLKPTGTKSIKALLKNYRLSDDIAFRFSDKNWSEWPL
                     HADTFASWVHKVAGSGDVINLFMDYETFGEHQWESTGIFDFMDHMPREILKHPDFGFM
                     TPSQVVDAYTPRGVYDAHEFISWADTERDLSAWLGNNMQQEALNKIYSISDRVLGSGN
                     KDLIDVWGKLQTSDHFYYMSTKFWSDGDVHKYFSPYNSPYDAYIFYMNAFSDFESTLT
                     EEIKPNRKSAAKKPAAPRKKKPAAE"
     gene            complement(8008..9816)
                     /gene="rpsA"
                     /locus_tag="PKU82_08125"
     CDS             complement(8008..9816)
                     /gene="rpsA"
                     /locus_tag="PKU82_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOZ82877.1"
                     /translation="MSEVKHNVPPTDFDWDNVGKTADIYKSDDRQRMEAMYDQTLNTL
                     TEHEVKMGSIISISDKEVVVNIGFKSDGMVPLSEFRHMPDLKVGDEIEVYVETREDKN
                     GQLILSHKKARALKSWERVNEALEKDEIIQGYVKCRTKGGLIVDVFGIEAFLPGSQID
                     VKPIRDYDVFVGKTMEFKVVKINHEFKNVVVSHKVLIEEELESQKSEIMSKLEKGQVL
                     EGTVKNITSYGVFMDLGGVDGLLHITDISWGRINHPDEVLKLDDKINVVILDFDEDKK
                     RIALGLKQLTPQPWEALDQNIQVGDKIKGKVVVLTDYGAFIEVIPGVEGLIHVSEMSW
                     SQHLRSPQDFLKVGDEIETVILTLDREERKMSLGLKQLMPDPWVNIMEKYPVGSKHTA
                     KVRSLTNFGVFVELEEGIDGLIHISDLSWAKKIKHPAEFTKVGENIEVVVLEVDSEHR
                     RLSLGHKQLEENPWDTYETIFRVDTVHSGVVGKVTDKGATIIFPSFGVEGFAPYRHLM
                     KENGTPAKAEETLDFKVIEFSKENKKIVVSHSRLFEEAKASEKMARDNEKAKDESDAF
                     KSVKKIKDSQEKTTLGDLSVLSALKDEMKKNEAENN"
     gene            complement(10053..>10355)
                     /locus_tag="PKU82_08130"
     CDS             complement(10053..>10355)
                     /locus_tag="PKU82_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOZ82878.1"
                     /translation="FAIYYKWENNVFEYDVCMPLAAAGKSAGNIVAGEVKTGKYLMTQ
                     YFGNYDNSNKAHEALVNYAKDNSIKISDAPLEFYITDPSVEKDTAKWQTDIYYQIN"
BASE COUNT         3396 a         1877 c         1789 g         3293 t
ORIGIN      
        1 gatctgcact gtgaatatat ggtagaaata ggggcaataa aacaatgatt gttccaataa
       61 ttgataaagt atagttcaat aaagcctttt tatttaattc gaatggtagt tttaaattaa
      121 caataaatgc tgcaataaaa agaaacataa taaagtaagc taagaaaaaa cctaaataga
      181 ttccacaata aaactgatat gctaaaccca ataataaaaa agtaaaatat ctacttttca
      241 aggtttgaat aaacctgatt gaccaataga taactaaggg tgcaataaat ctaggaaaaa
      301 cctgaatatg aaaaatttgc ccatggttgt acaatccaaa tccataaata aatgcacagc
      361 ttgctgcaat gagattatta atgccgaatt tttttagaac gtaaaaacat gaccaatagt
      421 tcaaaaggaa aagcacaatg atccaaagtt gaaaagatgt ttcacgatca acattaaaaa
      481 ctcggaagat ggcatatata ggcattgtac caattaaatt atccgatagt gcaattacat
      541 tcttataagg atacattgaa gaggcatccc aaaacccatg aatctgatca gttaaatatt
      601 gatatccatg ctcaagaata taatagttaa aacgggcatc acctaaatca ccgggaatgc
      661 gccccatatc ccatccaaaa tagggcatca ctccaaaata aatccctgct aacatcataa
      721 aaacagggat taatttattt aatgaaattt tcaggctctc ggtcttcatc tgtttattag
      781 ctaaaattaa aataaaaaat taatggctta atccatatct aacaatacac cacaatgctc
      841 tgaagccatc tttccatcca atcttttttc cttcggcata ggtccgtcca tagtacgaaa
      901 ttccaacttc ataaatacgt acattaggta ttcttgcaat tttagcagtt acttccggtt
      961 caaaaccaaa tcttttttct ttgagcttta gtgattgtac catttttgta ttaaacattt
     1021 tatagcaagt ttccatgtct gttaggttca aattggtgaa catattgcta caaaatgtca
     1081 agaatttatt tccgatggaa tgccagaaga ataaaattct atgtggctta ccacctaaaa
     1141 aacgtgatcc ataaactaca tctgcaaaac ctgtaagaat tggttttaac aaaatattgt
     1201 actcttccgg atcatattca agatcggcat cctgaatgat taaatattct cctgttgcca
     1261 attcaatgcc cttatgcagt gctgcgcctt tacccatatt aacaggctga ttaaaaagtc
     1321 tcatgtcagc ctctgggtga tctgtaatat atttctcaat tacctctttg gtagtatctt
     1381 ttgaacagtc gtttacaaga ataatttctt tgctgatgtt attagtcagt ttaactgcta
     1441 aaactttgtc aagaattaaa tgaatggttc gcgcttcgtt atatgcagga ataatgatgg
     1501 aaagtttctg tatcattttg ctttaatgtc aattaccagg gatttttgaa atcatcggta
     1561 ttaaactttt tatttcggtt gctaaaatag gttaattcat ctgatttttt ttgccgatag
     1621 aattgattgc cctttccaaa cattttgcgt atcaatgctg ttggccaaaa tataccgaag
     1681 taaataataa aaagtgcaat ttttgatatt aatcttccaa tagaagtagt tactaaagac
     1741 caaaccttat aaataatttt tgcaagcgga aaaacaaata ataaaataat cagtaataaa
     1801 gccgcaacta ctacaagcca atccaagctg ttaaaatttt ctgctttata cttctgataa
     1861 agaaaaacca ttaataaaat gaggcatatt gaagaaaaag tttgccaaat ttgcttattc
     1921 aacataatta aaacagggtg taaataaacg gtgcaaatgc aggattgctt ccggcaatta
     1981 tcaatacact cattaataga atgacaataa ttaatggtgc cagccagtat ttttttcgct
     2041 gaattataaa tctgaaaaga tctgatagta attccattga cttgagtgtt acaaatatac
     2101 aatatttaaa tttcttttgt tatatacagt gtaaggcact attgtggcaa atgaaatgct
     2161 atttttgcag actgaaaaaa atatccggaa tgttgcttaa acaggaaatt gagaaacgaa
     2221 gaacttttgc aataatatcg caccccgatg caggtaaaac aactttaaca gagaagttgc
     2281 tgttgtttgg tggtgctata caggttgcag gtgcagttaa atcgaacaaa ataaagaaga
     2341 ctgctgccag cgattttatg gaaattgaaa aacaaagagg tatttctgtt gcaacatcag
     2401 tgatgacttt cttttataaa aactgccaga taaatttatt agacactccg ggccacaagg
     2461 actttgctga ggatacatac agaacattaa ccgctgtgga tagtgtaatt cttgtaattg
     2521 actctgtgaa aggggtagag ccacaaacag aaaagttgat ggaggtatgc cgcatgcgaa
     2581 atactccagt aattattttc atcaataaga tggatcgtga aggtcagaat ccatttgatt
     2641 tattagatga gttggaagaa aaacttttta tccatacacg tccactaagt tggcccatca
     2701 gtagcggatc tacttttcag ggcgtttata acttacacaa taaaagtctg atgcttttta
     2761 gagcaaacca aaaggcttct gacagtgatg ttcttccgat taaagatatg caagacccac
     2821 tacttgacaa acagcttggg cgctatgttg atcagcttcg tgaagatgtt gagttgatag
     2881 aaggtgttta tgagccattc aacaaacaac tctatagtga aggcttactt gcaccggtat
     2941 tttttggtag tggtattaat aattttggtg ttaaagaact gttagatact tttattgaaa
     3001 ttgcgcctga gcctaaatcc cgacagacag accaacgtac agtgacacca gaagaaagta
     3061 agttctccgg gtttgtgttt aagatacatg ccaatattga ccctcgtcat cgtgacagaa
     3121 tagctttttt aagagtatgt tccggcatgt ttgaacgtgg taagttttat catcatgtca
     3181 ggttgaataa agatttacga ttcaacaatc cggcaacttt tatggctcaa gaaaaaagta
     3241 ttgtggagca agcttttccg ggtgatgtta taggacttta tgatacagga aactttaaaa
     3301 ttggtgatac acttacagag ggggaggaac tgatgtttaa aggtattccg agtttttcac
     3361 cagaaatttt taaagaactt atcaataccg accccatgaa aaccaagcaa cttgaaaaag
     3421 gaattgaaca gctaacggat gagggagtgg cgcagttgtt tattcagaat ggtggtaaca
     3481 gaaaaattat tggtacagtt ggcgaacttc agtttgaagt tattcaatac agacttcaga
     3541 acgaatatgg agcatcttgt aaatttataa cacagaatat atcaaaggcc tgttggctca
     3601 cttcaaaaga caagaataag ctagatgaat tcatgcgctt taaaatgaac aacattgcat
     3661 tcgataaaga tcaaaatcct gtttatcttg ccgactcaca atggatgtta gaccagatga
     3721 taaagaatta tcctgaagta caatttcatt ttaccagcga atttaaaatg gaagttgctg
     3781 tttaattctg cctaagtagt cctatcagac acataagtgc ttttttattc tgagaattaa
     3841 aatcatgtaa agttgcaatt ggatatgcaa ttgagagttt tataaaattc attttccggc
     3901 atctcggttt tacatccttg atttcagcca atggaatgta gttgtcttta tgtaaatcat
     3961 agcgccaagg tttttcattg atacacaaca atacattttc tgatttagag gattgcattt
     4021 tatcatagtc aagcatattc aaaaatttgc cacttaaatg aagtgtcaca ctgtaatgat
     4081 tagcccacca gaacaaaaag cgaattgcga aagtctcctc cttattaaac catcccggag
     4141 catcacaatt tatccaaggt aacccttcaa gattttctcc cctgtagatt ttgctgcgat
     4201 ttataattgt agattctatt ttaaaatcac tttccagttt ttctttagcc atagtgccta
     4261 actctgcaaa gttactgtat accttatctg tgatggctct tttaatatta aaaaagtcag
     4321 cagagatggc atgctgtatt tcttctgatg aaaacataag tccgtgaaga tagcaattac
     4381 tttttcgttt tatttgtgtt gtaaattaat tgttgaatga aacttactat aatcagtgcc
     4441 agtgtacgag aagggcgcaa aagccatcgt gcagcattat tttttaaaaa ttttctgatc
     4501 aaaaaatatc aggcagatgt tagcattgcc gatctgaatg aacttaactt ccctatattt
     4561 gaagagcgac ttagatttca gaaaaacaag tctcctgaag ttgttcaatt tgcagaaaac
     4621 attaaacagt ctgatggtgt tttaataatt acgcctgagt ataatggagg ctatccggca
     4681 tcattaaaaa atgtaataga cctgatgtat gatgaatggc gcagaaagcc tatagccatt
     4741 gcaactgttt ctgatggaat gtttggagga atgcaagtaa ttacttcctt gcagtttatt
     4801 ttatggaaaa taagagcatg gactgtacct gctatgtttc caatgccgca gatagataaa
     4861 aattttgatg ttgacggaac accggttgat gttgcaggca cagaaaaaag agctacagta
     4921 ttttgtgatg aatggatgtg gtgtattgaa gcaaacagaa gaatgcagtt gaatcattaa
     4981 atgaatttaa accaaagtaa ttttaatttg tttattcctg ataatattca aaaaatgtat
     5041 tacatgacaa gtttaatcgc aataattttg cttacaggtt gtcagaatca ggcacagtca
     5101 ctacgggaaa tttctaattc tacaaatatg caaagcaaca tagccacaga cacagcaact
     5161 ttcggaacag gatgcttttg gtgtacagaa gccgtttttg aatctcttga aggagtaatc
     5221 tcggcaactt caggttattc cggtggcaat gttgcagacc caacctatga gcaggtgtgc
     5281 agtggcaaca caggtcatgc agaatgtatt caggtagttt atgactctaa aaaaatttct
     5341 tatgatgacc ttttgcaagt attttgggaa agccatgacc ctacaacatt aaacaaacaa
     5401 ggcgctgatg tgggtacaca ataccgttct gtaatatttt atcataaccc acagcaaaag
     5461 cagttagcag aatcgtacaa gcaaaaactg aacaacgaaa atacttttgg caagcctgtt
     5521 gtgactgaga taacagcagc aactacattt tatcctgctg aaaattatca tcaggaatat
     5581 tttaaactga atggtcacgc accatactgt cagtttgtta ttcaacctaa ggttgaaaaa
     5641 atcagaaaga tttttagttc aaaaattaaa aaataactga agctatttct ttttcaataa
     5701 tgctttgcgt agttgtgcgc ttgttttaca gtcgccatca tgactccaac ccggaggacc
     5761 gagtaagtac ataattttat ttttaacaga tgttttcttt ttcaaatcat gtgcaatagc
     5821 ctgccattcg tgaaatacaa tttttcccgg attgttagaa tgtaaattat ttgtcagacc
     5881 atacttaatg ggttcatttt ccaattccct ctgaaaagta ccaaacaatt tatcccagat
     5941 tatcaaaacc attcccatgt ttctgtcaag ataacaaaca ttgcttgcat ggtgaactct
     6001 gtgatgtgat ggagttacaa gaaaatattc aagccatcct aatttgccaa cggtttgcgt
     6061 gtgaaccaat attccaaaaa tctgtgtcgc gctataaata aacataatgt caatagcctt
     6121 aaagccaacg atgctaagtg gaataaaata aatgaaccga taaagtggtt gcaaaacaga
     6181 agaacggaat ccaacagtaa ggttgaactc ttcgctgctg tgatgagtga catgtacagc
     6241 ccagaatagg cgacaaaaat ggtctatccg atgcagccaa taatacataa aatcttctgc
     6301 aagaaataaa gctaaccagt aaatccatgc agtagggtag gagttaataa ttctgaaatc
     6361 gaaaaaataa ttcagaatta acagacttgc agtacgcatt ataatatcga gtcccatatt
     6421 cagcaacatg agatagagat tgacaagagt tccttttttt gaatagagtt gtaaatgatg
     6481 aaatgcagaa aaaaatattt cagcaccaat aactataatg taaataggtg ttgaaatcag
     6541 aatcagcaac gactctctga aatgttccat ataaaatact gaagtaaaat tttattcagc
     6601 agcaggcttc tttttacgcg gtgctgccgg ctttttcgca gcagatttcc tattgggctt
     6661 aatttcttct gttaaagtag attcaaagtc agaaaatgca ttcatataaa aaatataagc
     6721 atcgtaaggt gagttataag ggctgaaata tttatgtaca tcgccatcac tccagaactt
     6781 agtactcata taataaaaat gatcgctggt ttgtagctta ccccaaacgt caattaaatc
     6841 tttgtttcct gacccaagca ccctgtcact tattgaataa attttattga gcgcttcctg
     6901 ttgcatatta tttcccagcc atgctgaaag atcgcgttca gtatctgccc aactgataaa
     6961 ctcatgtgca tcatatacac cgcgaggcgt ataagcatct acaacctgtg atggtgtcat
     7021 aaagccaaag tcaggatgct ttaatatttc acgaggcatg tgatccataa aatcaaaaat
     7081 gcctgtgctt tcccactgat gctcgccaaa agtttcataa tccataaaca ggtttatcac
     7141 atcaccacta cctgcaactt tgtgtaccca acttgcaaat gtatcggcat gtagtggcca
     7201 ttcgctccag tttttgtcag agaatctaaa agcaatgtca tcgctcaaac gataattttt
     7261 aagtaatgct tttattgatt ttgttcctgt tggtttcaat aaatgattag ggtgtctgtc
     7321 tttcaataaa cgatcaacac cttcgcacaa aatcccttta aagcccattt tttcaataaa
     7381 ggcagcaagc tcattgttgt aaattaattc ggtatttctg aaaacagttg gcttctgttt
     7441 aaagtaatgt tcaattttct gatcatgctc ctttatttgt ctctcaaact cttctttgtt
     7501 atataaaaaa ctgagtgaat gaaaatatgt ttcagagagg aattctacac aacctgtttt
     7561 agacaaatca acaaatgact gaagaacatc cggtctgaac aattccatct gttctatcac
     7621 tgttccactt agtgaatagc ttattctgaa tttaccttta tgacgcttaa tcagctcaag
     7681 cattttttta ttggcaggaa ggtaacattt ctcagatacc ttgtccataa tccatttatt
     7741 tttttcgcca tcttcataaa aatggtcttt accaatgtcg aaaacactgt aatgttttat
     7801 tctgaagggc tgatgcacct gaaagtaaaa gcaaaccgat ggcataaatt tgtttttaaa
     7861 aatggtcgtt gtgccaaaat taaacttttg cctgaaacaa aagaaaatat cctgaaactg
     7921 tcatcataaa tttatgatta aagggatggt acataaatca aaaagccgct aaaagcggct
     7981 ttttgatttt attgatgaat aagcagatta attgttttct gcttcgttct tcttcatttc
     8041 gtctttaagt gcagaaagaa cgctcaaatc acccaaggtt gttttttcct gagaatcttt
     8101 gattttcttt actgacttaa atgcatcact ttcatcttta gctttctcat tgtcgcgcgc
     8161 catcttttca gatgcttttg cttcttcgaa caagcggctg tgagaaacaa caatcttctt
     8221 gttctctttg ctgaattcga ttactttaaa atcaagtgtt tcttcagctt tagcaggagt
     8281 accgttttct ttcatcagat gacgatatgg agcaaaacct tcaacgccaa atgatgggaa
     8341 tatgatagta gcacctttat cggttacttt tccaacaaca cctgaatgaa cagtgtcaac
     8401 acggaaaatt gtttcgtaag tatcccaagg attttcttct aactgcttat gtccaagact
     8461 taatctgcgg tgctctgaat caacttcaag aactacaact tcaatgtttt cgccaacctt
     8521 ggtaaactca gcaggatgct taattttctt agcccatgaa aggtcagaga tatgaattaa
     8581 accatcaata ccttcttcta actcaacaaa aacaccaaag tttgtcaggc tgcgaacttt
     8641 tgcagtatgc tttgagccaa caggatattt ctccataatg ttaacccatg gatcaggcat
     8701 aagttgcttt aagccaagag acattttacg ctcttcgcgg tcaagagtaa ggattacagt
     8761 ttctatttca tcaccaactt taagaaagtc ttgcgggctg cgtaaatgct gtgaccatga
     8821 catttccgaa acgtgaatta atccttcaac acctggaatt acttcaatga aagcaccata
     8881 gtcagtcaac acaacaactt ttcctttgat tttgtcgcca acctgaatgt tttggtcaag
     8941 tgcttcccat ggctgaggtg taagctgttt caagccaaga gcaatacgtt ttttatcctc
     9001 atcaaaatca aggattacaa cattaatttt atcatctaac ttcaacactt catcaggatg
     9061 gttgattcgt ccccatgaaa tatcggtaat gtgcaataaa ccgtcaacgc caccaaggtc
     9121 cataaataca ccataagacg tgatgttttt aactgtacct tcaagaacct gtcctttttc
     9181 aagtttagac atgatttctg acttttggct ttcaagttcc tcttcgataa gtactttgtg
     9241 agaaacaact acgttcttaa actcatggtt gattttaacc actttaaatt ccatagtttt
     9301 tccaacaaac acatcatagt cgcggattgg tttcacatca atttgcgaac ccggtaaaaa
     9361 tgcttcaata ccaaatacgt caacgataag tccgccttta gtacggcatt taacataacc
     9421 ctgaataatt tcatcttttt caagggcttc attaacacgc tcccatgatt taagagcacg
     9481 tgctttttta tgtgataata tcagttgccc gtttttatct tcacgggttt ctacatacac
     9541 ttcaatttca tcgcccactt ttaagtcagg catgtgacgg aattcgctca gtggaaccat
     9601 tccatctgat ttgaatccga tgtttaccac cacttcttta tcagaaatgc tgatgataga
     9661 acccattttt acctcatgtt ctgtaagtgt gttaagggtt tgatcgtaca tggcctccat
     9721 acgttggcgg tcatctgatt tgtaaatgtc ggctgttttg ccaacgttgt cccaatcgaa
     9781 atctgtcggt ggaacgttgt gttttacttc actcattaat tgttttgttt tttaataatt
     9841 gaaattgtta gactttattt attgtttatg aatatacctt ttaaaacggg cgcgcaaaga
     9901 taggtatttg ctttgataaa taaaactaat atgcctgata attattcagt tccggttgtt
     9961 gaaggttctg cagcctgaaa ttcccaaaaa accaataaaa aagggctaaa ccaatcggtt
    10021 taacccttaa tctttatgct tttagagcag gctcaattaa tctgatagta aatatctgtc
    10081 tgccacttgg cggtatcttt ctcaacagaa gggtctgtaa tgtaaaattc aagaggtgca
    10141 tcagagattt ttatgctgtt atccttagcg tagtttacga gtgcttcatg agctttgttg
    10201 gaattatcat aattgccaaa atactgagtc ataaggtatt ttcctgtttt aacttcgcca
    10261 gcaacaatat ttccggctga ttttcctgct gcagccaatg gcatacaaac atcatattca
    10321 aaaacattat tttcccactt gtaataaatt gcaaa
//
LOCUS       DAOOOL010000089        10263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_603_length_10263_cov_123.022531, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000089 DAOOOL010000000
VERSION     DAOOOL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_603_length_10263_cov_123.022531"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKU82_08135"
     CDS             <1..240
                     /locus_tag="PKU82_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOZ82879.1"
                     /translation="EIAMKYESYIAKEFEHTVKLNKLENLILHEDFNYDKIKSLSAEA
                     RQKLSVVKPQTLGQASRISGVSPSDISILMVYLGR"
     gene            308..1207
                     /locus_tag="PKU82_08140"
     CDS             308..1207
                     /locus_tag="PKU82_08140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ82880.1"
                     /translation="MELLNECPSCGTQDKIEYLTCKDFGYSGSYFTINQCVRCGLLFT
                     SPRPDEAEIGAYYKSDDYVSHTNKSKGIINKLYPIVRNIAVNQKVRLVKKLIPKNSYV
                     ADLGAGNGYFVKALLKAGFQTKGYEPSPEAIKLAKETFDTNLLPIESFYSSGNEEFNC
                     ITLWHVLEHVHTLKKTLNNIKLSLKKDGILIIAVPNPASKDAEFYKEFWAAYDMPRHL
                     YHFTPEALTSLIESFNFTLLDTKPMLFDSTYVSLLSERYKAKSSKPIQIIKAFYRGIH
                     SNMHAAIYTGNYSSLIYIFRKNG"
     gene            complement(1204..1926)
                     /locus_tag="PKU82_08145"
     CDS             complement(1204..1926)
                     /locus_tag="PKU82_08145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOZ82881.1"
                     /translation="MADNLNIEKVLFLDIETVPQFESYGSLNSADKELWDNKALHLKK
                     TDFDTAESLYPRAGIYAEFGKIVCISVGWFVIENSKRNLKVKSFYSDDETNLLLKFSE
                     LLKNQFNKSSHLLCAHNGKEFDFPYLCRRMLLNKIKIPDILNIGGKKPWEVQHLDTMD
                     MWRFGDYKSYTPLKLLTHIFNIPSPKDEIDGSDVWHVYWKDKDLKKIAKYCQKDVVAV
                     ARLILAFKNESVLNDSEIEILN"
     gene            2062..2517
                     /locus_tag="PKU82_08150"
     CDS             2062..2517
                     /locus_tag="PKU82_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOZ82882.1"
                     /translation="MIAEQIHELIKVYIADKDLFLVDLKVTPGKITVLVDKPAGITID
                     ECVLLNRYLYKEMESSGVFENKEFEVGSPGMDQPLKVIGQYKRRIGRPVKIILNDGKQ
                     HNGILVDANEQELQLLETIKEKIGGKKVEKQLTTTLPMQNIKETKLEFK"
     gene            2530..3768
                     /gene="nusA"
                     /locus_tag="PKU82_08155"
     CDS             2530..3768
                     /gene="nusA"
                     /locus_tag="PKU82_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOZ82883.1"
                     /translation="MDHAVLIESFSEFKDSKNIDRATLMSIMEDVFRAMVRKKYGEDN
                     KVDVVINTDKGDVEMYRNRTIVEDGMVEDDNTQIALSDALKIESDFEVGEDVTEQILL
                     DDFGRRAVLAARQNLVARIMELEKESIYQKYKDRVGEIVTGEVYQVWKKEIMVMDDEG
                     NELSLPKNEQIPADFFKKGDTVRAVVLRVEMQGGSPKIILSRTAPVFLERLFELEVPE
                     VFDGLITIKNIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INFTNNLSLYIQRALSPAKISEIKLDDDTKKAAVYMKPDQVSLAIGKGGNNIKLAGKL
                     TGFEIDVYRDSDVEDDEDVDLDEFTDEIEPWIIEEFKKIGCDSAKDVLAISIDDLVKR
                     TDLEEETIREVVRILNEEFE"
     gene            3832..6879
                     /gene="infB"
                     /locus_tag="PKU82_08160"
     CDS             3832..6879
                     /gene="infB"
                     /locus_tag="PKU82_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOZ82884.1"
                     /translation="MSDEKKDVRLSKATKDLGVGIDHIIEFLNKNGIQVEKNPNTKIS
                     ADAFSLLTKEFAPDMHARQEANEITREKQKRENTIIEVKDDSGKSGSESETDDELADK
                     LLSIKQQATEKQATAKAKETKEKTTKAKVVKAEEPAPVAEVDKPVETKEKAKEPEVVK
                     AKVSKLEGPKIQGKIDLEATEKKKKKTLKKEDSTAEEPKKTTAKKKAEVVAIEEEPKK
                     KKTTKKKEEEEVKQIEEVAAVTKEEEPIAIVETQPEVVIDATPVTEETDKTDTTEAEQ
                     KADDTTPPELFRTDREKLTGPTILGKINLPVDPPKKKPVATSSEDDRKKKRKRKDKKV
                     DIAKAGGPPHQGGAPRTGGGQRPGGGHGDFKRRNDFRNKPAEAKPEVTDKEIQDQIKA
                     TLARLSGGGKSKSSKLKKLKREQAQEELQSQLEQESNEKKVIKVTEFVTASELSKLLG
                     VPINQIISTCMSLGMVVSINQRLDAETLSVVAEEFGATVEFVSVEVQEAIKEVEDKPE
                     DLKPRAPIVTVMGHVDHGKTSLLDYVRKTNVISGEAGGITQHIGAYEVQLDSGKKIAF
                     LDTPGHEAFTAMRARGAKITDVAIIVIAADDSVMPQTIEAINHAQAAGVPIVFAINKI
                     DKPGANPEKIKEQLSKMNILVEDWGGKFQVQEISAKQGLNVDLLLEKVLLEAELLDLK
                     ANPNKNAIGTVVESSLDKGRGYIATVLVQSGSLKIGDVVLAGCYSGRVKAMFNERGNK
                     RNNAGPATPVLILGLNGAPQAGDQFHVMDDEREAREIANKRLQLQREQGIRTHKHITL
                     DEIGRRLAIGNFKELNVIVKGDVDGSIEALADSLLKLSTAEVQVNVIHKSVGAITESD
                     VLLASASNAIIVGFQVRPSVNARRLAEKEEIEIRLYSIIYDAINEIKAAMEGMLAPEF
                     EEKIVCNIEVRETFKIPKVGTIAGCMVLDGKMTRNTAIRVIRDGIVAYTGKLASLKRF
                     KDDVKEVSAGFECGLNIEGYNDIKVGDIIEGYEQVAVKRTLTV"
     gene            6914..7120
                     /locus_tag="PKU82_08165"
     CDS             6914..7120
                     /locus_tag="PKU82_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82885.1"
                     /translation="MSALTFQNVNAQCSVCRAGAESSVEQGYKAGKGLNKGILYLMAV
                     PYVMVGVGFYFFYNKIKKKETKEG"
     gene            7156..8193
                     /locus_tag="PKU82_08170"
     CDS             7156..8193
                     /locus_tag="PKU82_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HOZ82886.1"
                     /translation="MQLRNLVSKQLLKKRLTEETFNRKTVSFYRYLKIEDVENFRNRL
                     YIDFEALNIFGRVYVAAEGINAQISVPEQNWELFKNYLEAQEQLKNMPLKIAVEDNGK
                     SFYKLIVRIKSKIVADGLNDNTFDVTNVGNHLSAKEFNEAMQQSGTIVIDMRNHYESE
                     VGFFKDALKPDADTFRDALPQAIEMTKDNKDNKILLYCTGGIRCEKASAYFKHHGYKD
                     VNQLYGGIIQYAREVNAEHLENKFLGKNFVFDERMGERISDEIVSTCHQCGGKCDTHV
                     NCANDDCHLLFIQCESCKATYEGCCTPECKAIINLPEEEQKRLRKGRVKKQAHAVYKS
                     RLRPDLKKILK"
     gene            complement(8196..9329)
                     /locus_tag="PKU82_08175"
     CDS             complement(8196..9329)
                     /locus_tag="PKU82_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOZ82887.1"
                     /translation="MKETELKQNAAAYAELKKNISSETTPIFLDRKKEIFSDYDLATR
                     RAAYYRWKSTESLDKLLIAFEANAIKAGIKVLWAVDTAQAHTELNAVIDKFQQHKIHI
                     HTCYNELETEKLNQPSPNSIKNKISICDAIFVSAEPAMIACLSDKNFYESDVKIILAS
                     VDRLISSVNELELLMSLQSLQRNNQRESFSLISNAGNNSHKQEVYVLIFDNGRSKLLE
                     SQTERQMLHCIDCGICQTVCPVTESIGTTGGNENNYGPPSYIRAVFDGGQREYGHYAG
                     LCNMCGKCNVHCPVHIDFKNSFLHLRHENVQQKHRSQKERLFYMVWKKTMLKREFMNW
                     KTVNPLRYAVETLFLKSKTGVRKLPQPAAKSFNQMWREKMGMK"
     gene            complement(9335..>10263)
                     /locus_tag="PKU82_08180"
     CDS             complement(9335..>10263)
                     /locus_tag="PKU82_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOZ82888.1"
                     /translation="KIVFINQHMDATKIMTFDAYGPAVTIRWAGAKRAAPLGAVAVII
                     RSVNPSLDDFPHTGTLHYPDTIKKIPACAVSTNDAEKLSAMLKSDPQLKIKLKMNCMQ
                     LPDAPSHNVIGEIKGADANAGYLIVGGHLDSWDTGEGAHDDGAGVVQAMEVLNLLKKA
                     GIKPRHTIRAVAFMNEENGGKGGEYYAKNALEKNEKHIAAIESDCGGFTPRGFFLQGD
                     SLKKEKIRSWKNLFESYQIHSFNFDGGGADIGHLKNQNTFLLGLLPDTQRYFDIHHSP
                     NDVFEHVNRRELQLGAATMAAMIYLIDKYGLE"
BASE COUNT         3499 a         1764 c         1990 g         3010 t
ORIGIN      
        1 gaaatagcaa tgaaatacga aagttacatt gcaaaagagt ttgagcatac agttaaactt
       61 aacaaattag aaaatcttat tttgcacgaa gattttaatt acgataaaat taaatcactg
      121 agtgcagaag ctcgtcaaaa gctttctgta gttaaaccac aaacactagg gcaagcatcc
      181 agaataagtg gcgtttctcc atctgacata tctatcttaa tggtctattt aggccgataa
      241 taaaagtgtt gtttcacgtg aaactaaaga ctacctgatg gtggtctttt tttatttttg
      301 cccctgaatg gaattactta atgaatgtcc ttcctgtggg actcaagata aaattgaata
      361 cttgacctgt aaagattttg gttattcagg ctcgtatttt actattaatc aatgtgttag
      421 atgtggattg ttgttcacat cccccagacc ggacgaagca gaaataggtg cttattataa
      481 gtctgatgac tatgtgtccc acaccaataa aagtaaagga attataaata agttgtatcc
      541 aattgttagg aatattgctg taaatcaaaa agttagattg gtaaaaaaac tgatacctaa
      601 aaactcctat gttgcagatt taggtgcagg taatggatac tttgtaaaag cactcttaaa
      661 agccggtttc caaactaaag gttatgagcc atcaccggag gctattaaac ttgcaaaaga
      721 aacttttgac accaacttat tacccatcga atccttttac tcttctggta atgaagagtt
      781 taactgtatt acactttggc atgtattaga acatgttcat actctaaaaa aaacattaaa
      841 caatatcaaa ttatcactta aaaaagatgg tattctgatt attgcagtcc caaatcctgc
      901 ttctaaagat gctgaatttt ataaagaatt ttgggctgct tatgatatgc caagacatct
      961 ttatcatttt acgccagagg ctttaaccag cttaattgaa tcttttaatt ttacattgct
     1021 tgacacaaaa ccaatgttgt ttgactccac ttatgtttca ttattaagcg aacgttataa
     1081 ggctaaatca tcaaaaccca tacaaataat taaagcattt tacagaggta ttcattcaaa
     1141 tatgcatgcc gctatttata caggaaatta ttccagtctg atttatattt tcagaaaaaa
     1201 cggttagttt aaaatttcaa tttccgaatc attcaataca ctttcatttt taaatgcaag
     1261 gatcaatcga gccacagcta caacatcctt ttgacaatac tttgcgattt ttttcaagtc
     1321 tttatctttc cagtaaacat gccatacatc actgccatca atttcatctt taggactggg
     1381 aatattaaaa atgtgagtca gtaatttcag aggcgtataa cttttataat ctccaaaacg
     1441 ccacatgtcc atagtgtcta aatgttgcac ctcccaaggc ttcttacctc caatatttaa
     1501 gatatccgga atttttattt tgtttaaaag cattcttctg cacagatacg gaaagtcaaa
     1561 ttctttacca ttgtgtgcgc acaacaaatg tgatgattta ttgaattggt tttttaataa
     1621 ctcactgaat ttcaataata agtttgtttc atcatcacta taaaaagatt taaccttaag
     1681 gtttctctta ctattttcaa tcacaaacca cccaacagaa atacatacta ttttaccaaa
     1741 ctccgcataa atgcctgctc ttggatacaa actttctgct gtatcaaaat ctgtcttctt
     1801 cagatgaagt gccttattat cccacaattc tttatcagca ctgtttaaac tgccataaga
     1861 ttcaaactgt ggaacggttt caatgtccaa aaaaaggact ttctctatgt ttaggttatc
     1921 tgccatagta ttttggttta aggtattatc aatggtaaaa atatactttt catttcatat
     1981 ttagttattt tttatacttt tgtggcgtat taggtaagtg aaggggacaa tgtccccttt
     2041 tttattcagt acccattaaa gatgattgca gagcaaatac atgaacttat taaggtctat
     2101 attgctgata aggacctgtt tctggtagat ttaaaagtta ctcccggaaa aataactgtt
     2161 ttggttgata agccggcagg cataaccatt gatgaatgtg tattactcaa tcgttatctt
     2221 tacaaggaga tggaatcatc aggtgtgttt gaaaataaag agtttgaagt aggttcaccg
     2281 ggaatggatc agcctctgaa agttattggt cagtataaaa gacgaatagg tcgtccggta
     2341 aaaattatac ttaacgatgg taagcaacat aacggaattc ttgttgatgc caacgaacag
     2401 gaattacaat tacttgaaac aattaaagaa aaaataggcg gtaaaaaagt tgaaaaacaa
     2461 cttacaacta ctttaccgat gcaaaatatt aaagaaacta aattagaatt taaataatac
     2521 atcaatacaa tggatcatgc cgtccttata gaatcattca gtgagtttaa agactctaaa
     2581 aacattgatc gtgccacatt aatgagtatc atggaagacg tattccgtgc tatggtcaga
     2641 aaaaaatatg gcgaagacaa caaagtagat gttgtaatca ataccgataa aggtgatgtg
     2701 gaaatgtaca gaaacagaac cattgtagaa gatggtatgg ttgaagatga caacacacaa
     2761 atagcattga gcgatgcatt aaaaattgaa tcagactttg aagttggcga agatgttaca
     2821 gagcagattc ttcttgatga ctttggtcga agagcggtgc ttgctgcacg tcagaattta
     2881 gttgcccgta ttatggagct tgaaaaagaa tccatctatc agaaatataa agatcgcgta
     2941 ggagaaattg ttaccggtga agtttatcag gtttggaaaa aagaaataat ggtaatggat
     3001 gatgaaggta atgaattgtc actacctaaa aacgaacaga ttcctgccga cttctttaaa
     3061 aaaggcgata ctgtgagagc tgtagttctt cgggtagaaa tgcaaggtgg aagcccaaaa
     3121 attattttat cacgcacagc acctgtattc ctagaacgct tgtttgagct tgaagtacct
     3181 gaagtgtttg atggcttaat tacaataaaa aatattgttc gagagcctgg tgaacgtgct
     3241 aaggttgctg ttgaatcgta tgatgacaga atagatcctg ttggtgcttg tgtaggcatg
     3301 aaaggttcgc gtattcatgg tattgttcgt gaattacgca atgagaatat tgatgttata
     3361 aatttcacaa acaatttatc gctttacatt caacgtgctt tgagtccggc aaaaatttct
     3421 gaaataaagc ttgatgatga cactaaaaaa gctgctgtat atatgaaacc cgatcaggtt
     3481 tcattagcca taggcaaagg tggtaacaac attaagttgg ccggtaaact taccggattt
     3541 gagattgatg tttaccgcga ttctgatgtt gaagacgatg aagacgttga tttggatgag
     3601 tttactgatg aaattgaacc atggattatt gaggagttca agaaaatcgg ttgtgactca
     3661 gcaaaggatg tacttgccat ctctattgat gatttggtaa aacgcaccga ccttgaagaa
     3721 gaaactattc gtgaagttgt aagaattctg aatgaagagt ttgaataagc taaatttgca
     3781 gccaatatgc atgctgctat taatttttaa gataaaaaca tagactgttt aatgtcagac
     3841 gagaaaaaag acgtacgctt aagtaaagcc accaaagatt tgggtgtggg tattgaccac
     3901 attattgaat ttctaaataa aaacggaatt caagtggaaa agaaccctaa caccaaaatc
     3961 agtgctgatg ctttttctct gctgacgaaa gagtttgcac ctgatatgca tgcacgtcag
     4021 gaagccaatg agattacacg cgaaaaacaa aaacgcgaaa acaccatcat tgaagtaaaa
     4081 gatgattcag gcaaatctgg ttctgaatca gaaactgatg atgaattggc cgataaactt
     4141 cttagcataa aacaacaggc aacagaaaaa caagctactg ctaaagcaaa agaaaccaaa
     4201 gagaaaacaa ctaaagcaaa ggttgttaaa gcagaagaac ctgcacctgt tgctgaagtt
     4261 gataaaccgg ttgaaacaaa agaaaaagca aaagaacctg aagttgttaa agctaaagta
     4321 agtaagcttg aaggtcctaa aattcaaggt aagatcgatc ttgaagctac agaaaagaaa
     4381 aagaaaaaaa cgcttaagaa agaagatagc actgctgaag aaccaaagaa aactactgca
     4441 aagaaaaaag cagaagttgt ggctatagag gaagaaccga agaaaaagaa aaccacaaag
     4501 aaaaaagaag aagaagaggt aaaacaaatt gaagaagtag ctgctgttac aaaggaggag
     4561 gaaccaattg ccattgttga gacacagccc gaagtagtca ttgacgcaac tccggtaact
     4621 gaagaaaccg acaaaactga tactacagaa gctgaacaaa aagctgatga tacaacacct
     4681 ccggaattat tcagaacaga cagagagaaa ttaacaggac caacaattct tggaaagata
     4741 aacctgcctg ttgatccgcc taagaaaaag cctgttgcca catcatcaga agacgacaga
     4801 aagaaaaaga gaaaaagaaa agataagaaa gttgacatcg caaaggcagg tgggccacct
     4861 catcagggtg gtgcaccacg tacaggtggc ggacaaagac cgggtggcgg acatggtgat
     4921 tttaaacgca gaaatgattt ccggaataaa ccggcagaag ctaagcctga agttaccgac
     4981 aaggaaattc aggatcagat taaagctaca ttagcacgac tgagtggtgg aggaaagtcc
     5041 aaatcatcta aacttaaaaa acttaaacgc gaacaagctc aggaagaatt acaatcacaa
     5101 cttgagcagg aaagtaatga aaagaaagtg attaaggtaa ccgagtttgt aacggccagc
     5161 gaattgtcga agttgctcgg tgtaccgatt aatcagatta tatcaacctg tatgagcttg
     5221 ggtatggtgg tatccatcaa ccaacgctta gatgcagaaa cattaagtgt tgttgccgaa
     5281 gagtttggtg caactgttga atttgtttct gtagaagtac aggaagccat taaagaagtg
     5341 gaagataaac cagaagacct gaaaccacgc gcaccgattg taacagtaat gggtcacgta
     5401 gatcatggta agacctcttt attggattat gtcagaaaaa cgaatgttat ttcaggtgaa
     5461 gccggtggaa taacacagca cataggagcc tatgaagtgc aacttgacag tggtaagaaa
     5521 atagcttttc ttgatacacc tggtcacgaa gcatttaccg ctatgcgtgc acgcggagct
     5581 aaaataaccg atgttgccat tattgtaatt gctgctgacg acagcgtaat gccgcagact
     5641 attgaggcca taaaccatgc acaggcagca ggtgttccaa ttgtttttgc aataaataaa
     5701 attgataagc cgggagctaa tcctgaaaaa attaaggagc agctttctaa aatgaatata
     5761 ttggttgaag actggggagg taaatttcag gtacaggaaa tcagtgccaa acaaggactg
     5821 aatgttgact tgttgcttga aaaagtttta ctcgaagcag agttgctcga tcttaaagcc
     5881 aatccaaata aaaatgcaat tggtactgta gtagaatcat cattagataa aggaagaggc
     5941 tacattgcaa cagtattagt tcaatccggt tcattgaaga ttggagatgt agtacttgcc
     6001 ggttgttata gtggtcgtgt gaaagccatg ttcaacgaac ggggaaataa aagaaataat
     6061 gcaggaccag caaccccggt tttgattctt ggacttaatg gtgcgccaca ggccggtgat
     6121 cagtttcatg tgatggatga tgaaagagaa gcaagagaaa ttgccaacaa aagattgcag
     6181 ctacagcgcg aacaaggtat tcgtacacac aaacatatta cacttgatga aattggcaga
     6241 cgtttggcaa tcggaaactt taaagaactt aatgtaatcg taaaaggtga cgtagatggt
     6301 tctattgaag ctcttgccga ttcgctgctt aaactttcaa cagcagaagt gcaggttaat
     6361 gtcattcaca aatcagttgg tgccattaca gaatctgatg ttctcttagc atcagcatct
     6421 aacgccatca ttgttggttt ccaggtgcga ccttcagtaa atgcacgcag acttgcagaa
     6481 aaagaagaga ttgaaatcag gctttacagc attatttatg acgctattaa tgaaattaaa
     6541 gcggctatgg aaggaatgct tgcacctgag tttgaagaaa agattgtctg caatattgaa
     6601 gtgcgtgaaa catttaagat accaaaagtt ggaactattg caggttgtat ggtgcttgat
     6661 ggaaaaatga caagaaatac tgccatcaga gttatccgtg atggtatagt agcttataca
     6721 ggcaagcttg catcactcaa acgttttaaa gatgacgtaa aagaagtttc tgccggattt
     6781 gagtgtggat taaatataga aggttataac gacattaaag taggcgacat tattgaaggc
     6841 tatgagcagg ttgccgttaa acgaacatta actgtataat atgaaacggc ttttaatttt
     6901 attaattata ttcttgtcgg cacttacctt tcaaaatgta aatgcacaat gcagcgtttg
     6961 cagggcaggt gcagaaagca gcgttgaaca aggatacaaa gccggtaaag gtctcaataa
     7021 gggaatactt tatttaatgg ctgttccata cgtcatggtt ggtgtcggct tttatttttt
     7081 ttacaataaa atcaagaaaa aagaaaccaa agaaggttaa tcattatatc taactgttta
     7141 attacaacac gtaacatgca attacgaaac cttgtaagta agcaactcct caaaaaacgt
     7201 cttaccgaag aaacatttaa caggaaaact gtttctttct ataggtattt aaaaattgag
     7261 gatgttgaaa atttccgaaa tcgtctttat attgacttcg aagctttaaa catattcgga
     7321 agagtgtatg tagcagccga aggcattaat gcacaaataa gcgttcctga acaaaactgg
     7381 gaattgttta aaaattatct tgaagcgcag gagcagctta agaatatgcc attaaaaatt
     7441 gctgttgaag ataatggcaa gtcgttctac aaacttatcg ttcgcataaa atcaaaaata
     7501 gttgccgatg gtttaaacga caatacattt gatgttacta atgtgggtaa tcatctttct
     7561 gcaaaagaat ttaacgaggc tatgcaacag tcaggaacga ttgttattga tatgcgaaat
     7621 cattacgaaa gtgaagtagg attttttaaa gatgctttaa aacccgatgc tgatacattt
     7681 cgtgatgctt taccacaagc aattgaaatg acaaaggata ataaggataa taaaatttta
     7741 ttgtactgca ccggtggtat tcgttgcgaa aaagcaagtg cttattttaa gcatcatggt
     7801 tataaggatg taaatcaact ttatggtggc atcattcaat atgcaagaga agtaaatgct
     7861 gaacatttag aaaataaatt tctgggtaaa aactttgtgt ttgatgaaag aatgggtgag
     7921 agaataagtg atgaaattgt cagtacgtgt catcaatgcg gaggcaaatg cgatacacat
     7981 gtaaactgtg caaatgatga ttgccactta ttgtttattc aatgcgaatc gtgtaaagca
     8041 acttatgaag gttgctgtac tccagaatgt aaagccatta ttaaccttcc tgaagaagaa
     8101 caaaaacgac ttcgcaaagg gcgtgttaaa aaacaagcac atgctgttta caaaagcagg
     8161 ctaaggcctg atctgaaaaa aattctgaaa taaaattatt tcattcccat tttttcgcgc
     8221 cacatctgat taaatgattt ggcagcaggc tgtggaagtt ttcgtactcc tgtttttgat
     8281 ttcagaaaaa gtgtttctac agcataacgt aacggattaa ccgttttcca attcataaac
     8341 tctcgtttaa gcatcgtttt tttccaaacc atataaaaaa gtcgctcctt ctgcgaacgg
     8401 tgtttctgct gcacattctc atgacgcaga tgtagaaaag agttcttaaa gtcaatatgt
     8461 acagggcagt gtacattgca tttaccacac atattgcata atcctgcata gtgtccatat
     8521 tcacgttgtc caccatcaaa aaccgctctg atatatgatg gcggaccgta attattttca
     8581 ttgcctcctg ttgtgccaat actttctgtt acaggacata ctgtctgaca tattccacaa
     8641 tcaatgcaat gtagcatttg cctttctgtc tgactctcta ataatttgct tcttccatta
     8701 tcaaaaatca aaacataaac ttcctgctta tggctgttat ttcctgcatt agaaattaaa
     8761 gaaaaagatt ctctttgatt gtttctttga agcgattgta gcgacataag caactcaagt
     8821 tcattgacag aagaaatcaa tctgtcaacc gatgctaata ttatttttac atcactttca
     8881 taaaaatttt tatcagataa acaggcaatc attgccggtt cggctgaaac aaaaattgca
     8941 tcacaaatac ttattttgtt cttaatactg ttaggtgaag gctgatttaa tttttccgtt
     9001 tcaagctcgt tgtagcatgt gtggatatga attttatgct gttgaaattt atcaataaca
     9061 gcgttcagtt ccgtatgtgc ctgagcagta tcaactgccc ataaaacttt tattcctgct
     9121 ttaattgcat tggcctcaaa ggcgatgagg agtttgtcga ggctttctgt gcttttccag
     9181 cgataatatg cagccctgcg tgtcgccaga tcataatcag aaaaaatctc tttttttctg
     9241 tctaaaaata ttggtgtggt ttcagaagaa atattctttt tcagttcagc ataagctgct
     9301 gcattttgtt tcagctcagt ttctttcaac agcattactc caatccgtat ttatctatta
     9361 aataaatcat cgcagccata gttgccgcac ctaactgcaa ttcgcgccta ttaacatgtt
     9421 caaacacatc atttggcgaa tgatgaatat caaaataacg ttgtgtgtca ggcaacaagc
     9481 ctaacaaaaa agtattttga ttttttaaat gaccgatgtc cgcacctccg ccatcaaaat
     9541 taaaactgtg aatctgataa gattcaaaaa gatttttcca tgaccttatt ttttcttttt
     9601 tcaaggaatc accttgtaaa aagaatcctc ttggagtaaa acctccgcaa tcagattcaa
     9661 tagctgcaat atgtttttcg tttttttcca gagcattttt tgcatagtac tctccgcctt
     9721 ttcctccgtt ttcttcattc ataaaagcaa cagccctaat agtatgacgt ggtttaatac
     9781 ctgctttttt aagcaggttt aatacttcca tggcttgtac cactcctgca ccatcatcat
     9841 gtgcaccttc gcctgtatcc catgagtcaa gatgtccacc aacaattaga tatcctgcat
     9901 ttgcatctgc gccttttatc tcaccaataa cattatgcga tggtgcatca ggaagttgca
     9961 tacagttcat cttcagcttt attttaagtt gcggatcgct tttaagcatt gctgataatt
    10021 tttcagcatc atttgtactt actgcacaag cagggatttt tttaattgta tcaggataat
    10081 gtaatgtgcc tgtatgtgga aaatcgtcta atgaaggatt tactgatcga ataatgacag
    10141 ccacagcacc taatggtgca gcccttttag ctcctgccca gcgaatagta acagcaggtc
    10201 cgtaggcatc aaatgtcata attttagttg catccatgtg ctgattgata aaaactattt
    10261 tac
//
LOCUS       DAOOOL010000090        10230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_606_length_10230_cov_74.951941, whole genome shotgun sequence.
ACCESSION   DAOOOL010000090 DAOOOL010000000
VERSION     DAOOOL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_606_length_10230_cov_74.951941"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKU82_08185"
     CDS             complement(<1..1166)
                     /locus_tag="PKU82_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ82889.1"
                     /translation="MKKPHISLLVFLLFILQGAFAQDLSKINFTLAKKLSGPFSVTRP
                     VALFAQGDVGIIQNYVQQNSGKVKFVAGDIVSLVLPLGAVNGLIALPSVEAVEDNGMQ
                     LVALDDSMRVRNNINPVQNGMAPLPQGYDGSGVVMGFIDSGIDFSHPDFKHANGSTRV
                     LYIWDHNLTVGNAPMPYNYGTEFTAADIDAGNASAHVDNSNGHGTHVTGMGAGNGLAL
                     NDHKGAAPAADIISVCVNWNLSDNDWLTTVADAVNYIYSKAYALNKPCVINISAGTYY
                     GSHDGKDLQAQAISNLISQRNGRSLVCAAGNAGNIPIHVQHLHTAANDTSFTWFNFNP
                     AYGNSIYLEMWADVANFTQMKFAVGADNVTSGYELQTMGNYYTVSQNLGTIHTDT"
     gene            1364..3793
                     /locus_tag="PKU82_08190"
     CDS             1364..3793
                     /locus_tag="PKU82_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ82890.1"
                     /translation="MIRKYSLFLLLFLQIQILNAQDKVKRFTISGYVKDASTGEALIG
                     ANVYLRETMKGVQTNTYGYYAITVDASDSVTLMSSFVGYNDFQQRMLIVKDVKVNVNL
                     HNKYIETQEVVITAKKEDQNVNSTQMGTVQLDVEQIKTLPAFMGEVDILKTIQLLPGV
                     QSAGEGNTGFYVRGGGPDQNLILLDEALVYNASHLFGFFSVFNSDAINNVSLIKGGMP
                     ANYGGRLASVLDISLKEGNNKEFKAEGGLGPIASRLTLQGPIQKDKSSFIVSGRRTFI
                     DLFQRPPFVKKGSNAANNTYYFYDLNAKINYQLSDKDRLFLSGYFGRDVFNFKSADTG
                     FKFRIPWGNATAVLRWNHLFSDKLFVNTSIIFSDYNFAFNAKQESFSFNIFSGIRDYN
                     AKIDFSWFPIITHNVKFGVNYTYHRFTPTSAEASQDSTEFDLGGVNRLYAHEAAAYIN
                     DDWTISDKFSVSAGLRYSLFDFTGPFTRYVKNEDNETIDTIYYKTNETVGFYHGLEPR
                     ISAKFSLDKNSSIKASFTQNYQYVHLASFTSVTLPTDVWVPSSDRVKPQDGRLYAAGY
                     FRNFHQNMFETSVEVYYKTMRNQIEYREGAQADDDVKNNTDNNFVFGKGWSYGAEFFI
                     KKAKGKLNGWVGYTLSYTDRKFPDIMNGKTFPAKYDRRHDISVVVSYNASKRWTFGLT
                     WVYATGNATTLPVSRYFMFGPLDLQGVVSSGLPNMSNGQIYDVYGDRNSYRMAAYHRL
                     DLSATYIKKKTKRFESSWNFSIYNVYSRMNPYFIYFNNEVDKETGEFKIQAKQVSLFP
                     IIPSVTYNFKF"
     gene            3799..4677
                     /locus_tag="PKU82_08195"
     CDS             3799..4677
                     /locus_tag="PKU82_08195"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOZ82891.1"
                     /translation="MKINLIKSFLLIFSATNLFISCEKDITVNLPKQKQQYVVEGYIE
                     NGLPAYVMLSKTADYFSPVDSAALQQYMVKNASIVISDGDITDTLLQVDPSIGYFYFS
                     PKIIGQTGKSYSLRIITPDNELLTAETKMLPAVPLDSVWFKIQEGKDSLGYVWAHLKD
                     PDTLGNCYRWFAKRLNKDKMFIAPSGSVFEDKFINGKGFDFAYNRGHLPNSTAVEDEN
                     EEAGFFKAGDTIMVKFCTITEDSYKFWRQAESQAGSSGSPFGSPAVFPTNIKGGIGIF
                     EAFNPTFYTVIAKKPD"
     gene            complement(4685..5983)
                     /locus_tag="PKU82_08200"
     CDS             complement(4685..5983)
                     /locus_tag="PKU82_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ82892.1"
                     /translation="MIKVRLTVLICFFFVQAQCQQVFTLQQAIEKALNDNFSIRIARN
                     QSEILNNNATLGNAGMLPQVNLNGSYVKANNNVVQELSNGTSTEKQNAGQSTIAGSAV
                     LEWVLFDGLTMFSEYNKNSALSKQGKEQLKANIEDVIENVSKSYFTVVKEKLLLSSIN
                     ETLKFYADRLQIADSKYKIGSASKLDYLQSKVDYNAMLSQKLNKETDVNNAMVELNRL
                     LQYSADTLLQTEDTITFVTMPAMNDLITGLQQNTAMQVSKLNVEVNRLQLNSIKGLRL
                     PVITGLAGYYYNENRNDAGLFSLNRTNGPQFGITARYNLFNGFNNSRQISNTKLNYIN
                     SQIEFQQTRTNQIADLNNTWNAFQKSQEILNLETENTAAARENASVYLASYRLGSANL
                     LQLNQSQESLYDALVRLVNAQYVNKTTEIHLLRLAGKLIK"
     gene            complement(5976..9041)
                     /locus_tag="PKU82_08205"
     CDS             complement(5976..9041)
                     /locus_tag="PKU82_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ82893.1"
                     /translation="MDLSEISVKRPVLAIVMSLVILLFGVISYTFLGVREYPSIDPPI
                     INVRTSYPGANADIIESQITEPLEKVINGIQGIRSISSTSSQGTSNITVEFNLNSDLE
                     AAANDVRDKVSQAVRTLPRDIDGLPVVTKSDANADAIISLTVQSNTRNTLDLSDYAEN
                     VIGQQLITIPGVSTLQIWGQRKYAMRLRIYPDKLAAYKLTVQDIQNALNRENVELPAG
                     KIAGDNTELVVRTTGQLHSEDDFNNMIIFNDGDQLIRFKDVGLAELGPENEETILRES
                     GIPMVGIALVPQPGSNYIEIADEFYKRLEKIKVDLPSDIKIGIALDNTTFVKKSITEV
                     EETLLIAITLVVIIIYLFFRDWLVAIRPLIDIPVSLVGAMFIMYIAGFSINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKIEAGLSPNKAAIEGTKEIFFAVVSTSVTLAAVFLPVV
                     FMEGFVGRLFREFGVVIAGAVLISAFVSLTLTPMLNARLVRKDKKHSKFYEKTEPFFQ
                     RMNQAYENSLKWFMEARWRAWTILGSAVALIFILGRSLPSELSPLEDRNWLRLLITAP
                     EGASYEYTDNFIVKISDVIEDSIPEKNGLLTVTAPGFTGAGAVNSGFARIVLTTPDKR
                     ERSQQEIADQLSGITRKMTDARVFVLQEQSLSAGGSRSALPVQFVLQNQEFDKLREVV
                     PAFLDKANKSPVFQGVDVNLKFNKPELNVSIDRAKANALGVSVDDIAQTLQLAFSGQR
                     FGYFIMQGKQYQVIGQVDRENRDVPYDLSKLYVKTNSGGLVQLDNLIEIKEISSPPQL
                     YHFNRYQSATISAGLAPGYTIGDGINEMKHIGDSLLDESFSTDLTGPSRDFAESSSNV
                     WFAFVLAIILIYLVLAAQFESFVDPFIIMITVPLAICGALISLWYFNQTLNIFSQIGI
                     IMLIGLVTKNGILIVEFINQLREKGVERSEAIIHGSVSRFRPILMTSLATSLGALPIA
                     LALGAGAKSRMSMGIVIVGGVLFSLIFTLYIIPAMYSVFSRKKEMVSYD"
     gene            complement(9046..10101)
                     /locus_tag="PKU82_08210"
     CDS             complement(9046..10101)
                     /locus_tag="PKU82_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ82894.1"
                     /translation="MKASRFILIVLIIAGLIAVKLIFFPGTKSSKFGGKANAAAVPVT
                     GFIARSQPLESNLKANGTINAYEQTDLRAEISGRIVAILFKDDQPVKKGQLLIQIYND
                     DLKANLSKSTAVLKQTQDRLKRQEKLLEIGGVSTEEVEETQTEAATQKAEIDYLNFQI
                     AKTEIYAPFDGIVGIRDISIGNYVNSSTVIATVYQVNPLKIDFTVPEKYLPYISKGMK
                     IRFTTDVNKKEYEGEISAINPMIDPSTRMVRLRAYTSNSDHSLTPGSFAHIQVDFKIT
                     ENAILIPTQSVIPILKGKKVFISKNGKADEKIIETGERTADMIEVTSGLTAGDTVLTT
                     GILQVRKGSDVKFSEVK"
BASE COUNT         3252 a         1973 c         1744 g         3261 t
ORIGIN      
        1 gtatctgtgt gtattgttcc caaattttga gaaacagtat aatagttacc cattgtctgt
       61 aattcataac cggaagttac attatctgca ccgactgcaa atttcatctg agtaaagttt
      121 gccacatcag cccacatttc taaataaata gaattaccat aggcagggtt aaaattaaac
      181 catgtaaacg atgtatcatt agcagcagta tgcaaatgct gaacgtgaat aggtatattt
      241 cctgcattac cggctgcaca aacaagtgag cggccattac gttgggaaat gagattagag
      301 atagcctgtg cctgtaaatc ttttccatca tgagaaccat aataagttcc cgcactgatg
      361 ttgattacac atggcttatt aagtgcataa gcttttgaat aaatataatt cacagcatcg
      421 gcaactgttg ttaaccaatc attgtctgaa aggttccagt ttacacaaac agaaattata
      481 tcggcagcag gtgcagcacc cttatggtcg ttcaatgcaa gaccattgcc ggcacccata
      541 cctgtaacgt gtgtgccatg tccattgctg ttgtcaacat gagcggaggc attgcctgca
      601 tcaatatctg ctgcagtaaa ttctgttcca taattatagg gcattggtgc atttccaact
      661 gttaaattat gatcccagat atacaacaca cgtgtacttc cgttagcatg tttgaaatca
      721 ggatgagaaa aatcaatacc tgaatcaata aatcccatta ctacgccact gccatcgtag
      781 ccttgcggca aaggagccat accattttga acaggattga tgttgttgcg cacacgcata
      841 gagtcgtcta aagctacaag ctgcattccg ttatcctcaa ctgcttctac acttggaagt
      901 gcaattaatc cattaacagc acctaagggt aaaaccaatg aaactatatc tccggctaca
      961 aactttactt taccgctatt ttgttgaaca taattctgaa taatgccaac atctccttgt
     1021 gcaaataatg ctacagggcg tgttactgaa aacggcccgg acaatttttt agcaagtgta
     1081 aaatttatct tgctcaaatc ctgagcgaat gcaccctgaa gaataaataa taaaaatact
     1141 aagagtgaaa tatgtggttt tttcattact attttattgg tcaacaattt taaataaaat
     1201 attctgttta gataatgagg ggcaatacaa ttatcaacaa atttacaata aaaggttttt
     1261 ttacaatatt tatttcagat taaaaatata tgttaacatt ttaagtattt gaatctgaat
     1321 ttaagaatga aatgataagt ttgcaacccc gtataatcaa tttatgatca gaaaatattc
     1381 gctcttttta cttctttttc tacaaattca aatcttaaac gctcaggaca aagtaaaacg
     1441 gtttactatt agcggttatg ttaaagatgc ctctacaggc gaagcattaa ttggagctaa
     1501 tgtatatctg cgtgaaacga tgaaaggtgt tcagactaac acttatggct actatgcaat
     1561 taccgttgat gcctctgata gtgttacact gatgagctct tttgtaggat ataacgattt
     1621 tcagcaacga atgcttattg ttaaagatgt caaagtaaac gtgaatctgc acaacaaata
     1681 cattgaaacg caagaagtag ttatcactgc caagaaagaa gatcagaatg ttaacagcac
     1741 gcaaatgggt acagttcaac ttgatgtgga gcagataaaa acactgcctg catttatggg
     1801 tgaagtggat attttaaaaa ctattcaact gctgcccgga gtgcaatctg ccggtgaggg
     1861 gaacacaggt ttttatgttc gcggtggtgg ccccgatcaa aatttaatat tattggatga
     1921 agcattagtt tataatgcct cacatttatt tggttttttt tcggtattta attccgatgc
     1981 catcaataac gtttcattga ttaaaggtgg catgcctgca aactatggcg ggcgacttgc
     2041 atctgtatta gacatttcgc ttaaagaagg aaacaacaaa gagtttaaag cggaaggtgg
     2101 attaggaccc attgcctcac gacttacatt gcaaggcccg attcaaaaag ataaatcatc
     2161 attcattgta tcaggaaggc gtacatttat tgatctgttt caacgtccac cgtttgtaaa
     2221 aaaaggttcc aatgctgcta acaacaccta ttacttctat gatttgaatg ctaaaataaa
     2281 ctatcagctt tcagataaag acagactttt tttaagcggc tactttggca gagatgtgtt
     2341 taacttcaaa agtgccgata caggatttaa gttcagaatc ccgtggggaa atgctactgc
     2401 cgtattacgc tggaatcatt tattcagtga caagttattt gtaaacacct caattatttt
     2461 ttctgattat aactttgctt tcaatgcaaa acaggaaagt tttagtttca atattttttc
     2521 aggcatcaga gattataatg caaaaattga ctttagctgg tttcctatta tcacacacaa
     2581 tgttaagttt ggtgtaaact acacctacca tcgctttact cctacaagtg cagaggccag
     2641 tcaggattca acagaatttg atttgggtgg agtaaacaga ctctatgcac atgaagctgc
     2701 tgcctacatt aatgatgact ggacaattag tgataaattt tctgtaagtg ctggcttacg
     2761 ttattcactt ttcgatttta cagggccttt tacacgctat gttaaaaacg aagacaatga
     2821 aactattgat actatttact ataaaacaaa cgaaactgtt ggtttctatc acggacttga
     2881 gcctcgcatt tcagctaaat tttcgttaga taaaaactct tccataaaag catcattcac
     2941 acaaaactat caatatgtgc acttggcatc gtttacttct gttacgctgc ctacagatgt
     3001 ttgggtaccc agcagcgaca gagtaaaacc acaagacgga agactgtatg ccgcaggata
     3061 tttcagaaac tttcatcaaa atatgtttga aacatctgta gaagtttatt acaaaaccat
     3121 gcgcaatcaa attgagtatc gtgaaggtgc acaggctgat gatgatgtga aaaataatac
     3181 cgataataat tttgtatttg gtaaaggatg gagttatggt gctgaattct ttatcaaaaa
     3241 agcaaaagga aaactaaatg gatgggttgg ttacacactt agctatacag atagaaaatt
     3301 tcctgatatt atgaatggaa aaacatttcc ggcaaagtat gacaggcgac atgatatttc
     3361 tgttgtggtg tcttataatg caagcaaacg ttggacattt ggtttgactt gggtttatgc
     3421 aacaggtaat gccactactc tgcctgtttc aagatatttt atgtttggtc ctttagattt
     3481 acaaggtgtt gtatcaagtg gattacccaa tatgtcaaac ggacagatat atgatgttta
     3541 tggcgacaga aattcttaca gaatggcagc ctatcatcgt ttagatttat ctgcaaccta
     3601 tatcaagaaa aagacaaaac gctttgaatc aagttggaat tttagcatct acaatgttta
     3661 cagcagaatg aacccctatt ttatttattt taataatgaa gttgataaag aaacaggtga
     3721 atttaaaatt caggcaaaac aagtttcgct gtttccaatt attccttctg ttacttataa
     3781 ttttaaattc tgatggcgat gaaaataaat cttataaaat catttctatt aattttttct
     3841 gcaacaaatt tattcatctc ttgcgaaaaa gatattacag ttaatctccc aaaacaaaaa
     3901 caacaatatg ttgtagaagg ttatattgaa aatgggttac cagcctatgt catgctttct
     3961 aaaacagcag attatttttc gccagttgat tccgcagcac tgcaacaata catggttaaa
     4021 aatgcttcca ttgttatctc tgatggtgat attactgata cactactaca ggttgaccca
     4081 tcaataggct atttttattt ttcaccaaaa attattgggc aaacaggtaa aagctattcg
     4141 ttacgcatca ttacacccga caatgaatta cttactgctg aaactaaaat gcttcctgcc
     4201 gtaccattag acagtgtatg gtttaaaatt caggaaggga aagattcatt aggttatgta
     4261 tgggcacatt taaaagatcc tgacacatta ggtaattgct atcggtggtt tgccaaacga
     4321 ttaaataaag ataaaatgtt tattgcacca tcaggctcgg tgtttgaaga taaatttatt
     4381 aatggaaaag gatttgattt tgcatataac cggggacatt tgcctaacag tacagcggtt
     4441 gaagatgaga atgaagaagc aggattcttt aaagccggtg ataccattat ggtaaaattc
     4501 tgcacaataa cagaagatag ctataagttt tggcgtcagg cagaatcaca ggcaggaagt
     4561 agtggcagtc cttttggttc gccagcagtt tttcctacaa atattaaagg cggtattgga
     4621 atttttgaag cttttaaccc gaccttctat acagtaattg ctaagaagcc tgattaaatt
     4681 ttctctattt aattaatttg cctgccaaac gcagtaaatg tatttcagta gttttgttaa
     4741 catactgtgc attaaccaac cgcactaagg catcatataa actttcttgt gattgattta
     4801 attgcaaaag atttgcacta ccaagacgat aactggcaag atacacactt gcattttcac
     4861 gggcagcagc agtattctca gtttctaaat taagtatctc ctgagatttt tgaaatgcat
     4921 tccatgtatt atttaaatca gcaatttggt tggttcgtgt ttgctgaaat tcaatttgcg
     4981 aattgatata attcaatttt gtattactga tttgtcttga attattgaaa ccgttaaaca
     5041 aattataacg tgccgtgatt ccaaactgtg gtccattagt tctgttcaaa gaaaacaatc
     5101 ctgcatcgtt gcggttttca ttataataat accctgcaag acctgtaatt acaggcaaac
     5161 gtaatccctt tattgaattt aactgtaaac gatttacttc tacattcagt ttgctcactt
     5221 gcattgcagt gttctgttga agtccggtta tcaaatcatt cattgcaggc atagttacaa
     5281 aagtgattgt atcttcagtt tgcaataaag tatcagcaga atattgtaac aaacggttta
     5341 actcaaccat tgcattattt acgtctgttt ccttattcaa cttctgcgat aacattgcat
     5401 tgtaatcaac ttttgactgt aaataatcca gtttagaggc tgaaccaatt ttatatttac
     5461 tatctgcaat ctgaagtctg tctgcataaa atttcaaggt ttcattaatg ctcgacaaca
     5521 acaacttttc tttaactaca gtaaaatatg atttacttac attctcaata acatcttcaa
     5581 tgtttgcctt cagttgctct tttccttgtt ttgagagtgc tgaattttta ttatactccg
     5641 aaaacatagt aagtccatca aataagaccc attccaaaac tgcactacct gcaattgttg
     5701 actgacctgc attttgtttc tctgtagatg tgccattact caattcctgc accacattgt
     5761 tatttgcctt aacatagcta ccattcaaat ttacctgagg caacattcct gcattaccta
     5821 atgttgcatt gttgtttaga atttcactct gatttcgggc aatacggatt gaaaaattat
     5881 cattgagggc cttttcaatt gcctgttgca gtgtaaacac ctgttgacat tgtgcctgta
     5941 caaagaaaaa acaaatcaat actgttaatc gtaccttaat cataacttac catttctttt
     6001 tttcgtgaaa aaacagaata cattgccgga ataatataca aagtaaatat cagagagaaa
     6061 agtactccac caacaattac aattcccatt gacatacgac tctttgctcc cgctcctaaa
     6121 gctaatgcaa tgggcagcgc tcctaatgag gtagcaagac ttgtcattaa aattggccgg
     6181 aaacgtgata cagaaccatg aataattgct tcagatcgtt ctactccttt ttcacgtagt
     6241 tgattaataa actcaacaat tagaattcca ttttttgtaa caaggccaat gagcataatg
     6301 ataccaatct gactgaaaat atttaatgtc tgattaaaat accaaagtga aatcaatgca
     6361 ccacaaattg caagtggaac tgtaatcatg ataataaatg ggtcaacaaa actttcgaac
     6421 tgtgctgcca acaccaaata gattaaaata attgcaagta caaatgcaaa ccaaacattt
     6481 gaactgctct ctgcaaaatc gcgtgaagga cctgttaaat cggtcgaaaa actttcatca
     6541 agtaaactat cgccaatatg tttcatttca tttattccat caccaattgt gtaacccggt
     6601 gcaagccctg ctgatatggt ggcagattga taacgattaa aatgatataa ctgtggtgga
     6661 ctactgattt cttttatttc aattaaattg tcaagctgaa caagacctcc actgtttgtt
     6721 tttacataaa gttttgaaag gtcataaggc acatcacgat tttcacgatc tacctgaccg
     6781 atgacctgat actgcttgcc ctgcatgata aaatatccaa atcgctgtcc gctgaaagct
     6841 aattgaagtg tttgtgcaat gtcatcaaca gataccccta atgcatttgc ctttgctctg
     6901 tcaatagaaa catttaattc tggcttgttg aatttcaggt tgacatccac accctgaaat
     6961 acaggacttt tatttgcttt atctaaaaat gcaggcacaa cctctcttag cttatcgaat
     7021 tcctgatttt ggagaacaaa ctgaactggt aatgcactac gtgatcctcc tgccgacaat
     7081 gactgttcct gcagaacaaa tactcttgca tcagtcatct tgcgtgtaat tcccgaaagt
     7141 tgatctgcaa tttcttgttg cgaacgttca cgtttatctg gtgttgtaag tactattcgg
     7201 gcaaagcctg aatttactgc tcctgcacca gtaaaacccg gtgcagttac tgttagcaat
     7261 ccgtttttct ccggtataga atcttcaata acatcagaaa ttttcacgat aaaattatct
     7321 gtatattcat aagacgctcc ttccggtgct gtaattaaaa gtcgcagcca gtttctgtct
     7381 tctaaaggtg aaagttcact tggcaaactc cttcctaaaa taaaaatcaa agcaacggct
     7441 gatcctaaaa ttgtccatgc acgccatctt gcttccatga accacttaag agaattttca
     7501 taagcctgat tcatccgttg aaaaaacggc tctgtctttt cataaaattt agaatgcttt
     7561 ttatcctttc tcaccaaacg cgcatttaac attggtgtta aggttaatga aacaaatgct
     7621 gaaataagta ctgcacctgc aatgaccaca ccaaactccc tgaagagtcg cccaacgaat
     7681 ccttccataa aaactacagg tagaaatact gctgctaacg ttactgaggt actgacaact
     7741 gcaaaaaata tttcttttgt tccttcaatt gcggctttgt tgggtgaaag tcctgcttca
     7801 atttttttat aaatattttc tgtaacaaca attccatcat ctaccaccaa tccggttgca
     7861 agcactatcg ctaaaagcgt tagcacatta attgaaaatc cagctatgta cataataaac
     7921 atggctccaa ccaacgagac tggaatatct atcaatggac gaatggccac aagccagtca
     7981 cggaaaaaca aatagataat gattacaaca agcgtaatgg caattaaaag tgtctcttcc
     8041 acctcggtaa ttgacttctt tacaaaagtt gtgttgtcga gtgcaatacc aattttaata
     8101 tcagaaggca aatcaacttt tattttttcc aaccttttat aaaattcatc agcaatttca
     8161 atgtaattac ttcctggttg tggcacaaga gcaataccca ccatcggaat gcctgattcc
     8221 cttagaatag tttcttcatt ctccggccct aattctgcaa gacctacatc tttaaaccga
     8281 ataagttgat cgccatcatt aaagataatc atattattga aatcgtcttc gctgtgaagc
     8341 tgtcctgtag tccgtacaac aagttcagta ttatcacctg ctatttttcc tgcaggcaat
     8401 tccacattct ccctattcaa tgcattttga atatcctgaa cggtaagttt atacgcagca
     8461 agtttatcag gataaattct taaacgcata gcgtatttac gctgtcccca aatctgcaag
     8521 gtgctaacac ctggaattgt aattaattgc tgaccgataa cattctctgc atagtcactt
     8581 aagtcaagag tattccgggt attactctga acggtaagcg aaatgatggc atcggcatta
     8641 gcatcagatt tagtaacaac cggtaatcca tcaatgtcac gtggcaaagt acgaacagct
     8701 tgagaaactt tatctcttac gtcattggct gctgcttcga gatcagaatt cagattaaac
     8761 tcaacagtaa tattacttgt tccctgactg ctagttgaag aaattgaacg aatgccctga
     8821 ataccgttta tgactttttc aagtggttct gttatctgcg actcaataat atcggcatta
     8881 gcaccgggat acgatgttct tacattaata attggtgggt caatagatgg atactctctg
     8941 acaccaagaa aagtataact gatgacaccg aataacagta tcaccaacga cataactata
     9001 gcaagaacgg ggcgttttac acttatctca ctcagatcca taaacctatt ttacctcact
     9061 gaatttaaca tcgcttcctt ttctaacctg caaaattcct gttgtcaaca ccgtatcacc
     9121 ggcagtcaaa cctgaagtaa cttctatcat atctgctgtt cgctcacctg tctcaattat
     9181 tttttcgtct gccttgccat ttttactaat gaaaactttc ttgcctttta aaattggaat
     9241 tacagactga gtaggtatca atattgcatt ttcagtgatt ttaaaatcaa cctgaatgtg
     9301 tgcaaaagaa cccggtgtta atgaatggtc actgtttgat gtgtaagccc gcagacgaac
     9361 catccttgtt gacggatcaa tcattgggtt aattgctgaa atttcacctt cgtattcctt
     9421 tttattcaca tccgtagtaa atctgatttt cattccttta ctgatataag gaagatactt
     9481 ctcgggaact gtaaaatcaa ttttaagtgg gttaacctga taaactgtag caataacggt
     9541 tgacgaattt acgtagttgc ctatactgat gtctctgatt cctacaatgc catcaaatgg
     9601 cgcataaatt tctgtcttgg caatctgaaa attcaaataa tctatttcag ccttctgagt
     9661 agcagcttcg gtttgtgttt cttctacctc ttcagtgcta acccctccta tttctaatag
     9721 tttttcctgc cgttttaacc tgtcctgtgt ttgctttaaa accgctgtac tctttgaaag
     9781 attcgctttc aaatcatcat tataaatctg tatcagcagt tgacccttct tgaccggttg
     9841 atcatcttta aacaatattg caacaatgcg tcctgaaatc tctgctcgta agtcagtctg
     9901 ctcataagca ttgattgttc catttgcctt taaattactt tcaagaggtt gcgaacgggc
     9961 aataaaccct gtaacaggaa ctgctgctgc atttgctttg ccaccaaact ttgaagattt
    10021 ggtgcccggg aaaaatatca acttaacagc aattaatcct gcaatgatta atactattaa
    10081 aataaatcgt gatgctttca taaattaatc aaaagaaata aaaggaacaa aaatatgatt
    10141 ctattttatg aattgattgt tttcaattga aacaattaac aaactttaga aaattttcag
    10201 caaagtacat tatcagttga gaattaatac
//
LOCUS       DAOOOL010000091        10195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_609_length_10195_cov_105.343393, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000091 DAOOOL010000000
VERSION     DAOOOL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_10195_cov_105.343393"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PKU82_08215"
     CDS             <1..1038
                     /locus_tag="PKU82_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOZ82895.1"
                     /translation="FARIYGYGVAGVIFFHFMDKLLRPFGLNGRSVIPLMSGVACAVP
                     AILATRTIQSWKERIVTIMVTPLMSCSARLPVYTLLISLVIPRYYVFGFINSQGLVMM
                     LMYLIGFVAAIFSAWLFKMFVKAKQRDFFIMELPVYRLPRWKNIFFVIFDKVKIFLFD
                     AGKIIIAISIVLWFLSSRGPGKDYAETEKLYEAKAELPETTQDELNQLQAQKLEHSYA
                     GWTGKIIEPAIKPLGFDWKIGIALVTSFAAREVFVGTMSTIYSVGSEGENLPIREKMM
                     AEKRPDGTPLYTLAVGISLMLFYAFAMQCMSTLAVVKRETGTWKWPIIQFVYMSALAW
                     VSSFAAFQLLS"
     gene            1066..1695
                     /locus_tag="PKU82_08220"
     CDS             1066..1695
                     /locus_tag="PKU82_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HOZ82896.1"
                     /translation="MAKVTLEFIEEALKKYVPHQSIRYCIQWIEEHQINLKITRSRNS
                     KYGDYRPPQRGFGHRISINHDLNPYAFLITFIHEVAHLNQWKRKKQIIIPHGKDWKEE
                     YRKLMIPILRENIFPPDVVAALNNYMTNPSATSCSDHDLLRALRNYDREEDKWITLEE
                     LNFGVKFKIRTGRTFIKKEKLRKNYSCIDCRSKSIYFINPITEVMPVDH"
     gene            complement(1689..3101)
                     /locus_tag="PKU82_08225"
     CDS             complement(1689..3101)
                     /locus_tag="PKU82_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ82897.1"
                     /translation="MKPAAVFNLLLKNFPFEPTNAQKHAFAGLSEFITENTAYKCFLL
                     KGYAGTGKTTVINAMIKTLYHYDFRVIMLAPTGRAAKVINHYTGDDASTIHRYLYEIK
                     SDKNGDIKTKVKPNKFKKQIFIIDESSMISNEDDKTLFKGASLLSDLVDHVYSQADNA
                     IVFIGDTAQLPPVGLQCSPALDQNFLQNNCQLQVRQYELKEVVRQQLHSGILANAHCT
                     RLLLEREEHFPRLKTSGFDDVNQVTSAELSEALSGYFPSGDDNTVTVLCRSNKQANRY
                     NEFIRKTFYGFEEVVEKNERLMVVKNNYYWHESDNDNAFIANGDEIKLIRLKKQEEYF
                     GFRFANATIELSDEKSTRLEVKLLLDTLNSESPALTYDEYKKLFDEVRNHYRKTDGKV
                     NMRKLREDPYLNALQVKYAYAITGHKAQGGQWETVFIDPGFMTEEMYGVDYMRWLYTA
                     ITRARKKVYLINVPDRFFVQ"
     gene            3313..4326
                     /locus_tag="PKU82_08230"
     CDS             3313..4326
                     /locus_tag="PKU82_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOZ82898.1"
                     /translation="MLSIDIHTHILPEKLPDWKSKFGYGGFITLDHHKNCCARMLRDD
                     GTFFREIESNCWDAKTRMNDCDVHGVHVQALSTVPVMFSYWTKPADGLAVSEFLNDHI
                     ATIVQQYPKRFVGLGTVPLQAPDLAIKEMERCKKIGLQGLQIGSHVNDWNLNDANLFP
                     FFQACAENNMPLFVHPWEMMGQKDMQRYWLPWLVGMPAETSRAICSMIFGGVFERLPN
                     LRVAFAHGGGSFPSTFGRIKHGFDCRPDLVAIDNASSPERYIGKFYFDSLVHDENMLH
                     YLINFTGANRIALGSDYPFPLGENIPGTLIKNAKLDLETKERLLGGTALEWLGLSKEM
                     FLR"
     gene            4452..5066
                     /locus_tag="PKU82_08235"
     CDS             4452..5066
                     /locus_tag="PKU82_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOZ82899.1"
                     /translation="MDIRERILESGLELFLAHGVRRITMDDIARHLSVSKKTIYACFD
                     DKDEIVSECCQGYLKNSRCEFEHVETESKDAIHEIFLTMEKLQAMFTTINPALFEDLQ
                     KFYPRVWSMYVQFKDKHVAEVIERNLLKGIEQKLYRPDINIKILVKLRIEEITLGMNT
                     TVFPLKNFKTQDVQVALLDHYLHGIVTVKGHKLINKYKNITDEE"
     gene            5078..6427
                     /locus_tag="PKU82_08240"
     CDS             5078..6427
                     /locus_tag="PKU82_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ82900.1"
                     /translation="MLNQIKFLTGLLLLISINIQAQQSDKKSYSFSLKQAIDFAFQNQ
                     TDIQNAVLDQKSAQSRANELLGIGLPQISASADITHFIDIPTTFVPAEFFGGEKGSYA
                     PVQFGQPYSSTAGLSASQLLFDGSYLVGVKASRMYADLMRKQVIQTKTEKAALISKAY
                     YNVQVNSERLQMLDANVKRLKKQLDEMQVMYNNGFIEKIDLDRISLAYNNIETEKSNA
                     DRLIVLAQNLLKFQMGLNVNDELTLTDKLEDIKVVADNNLNQNIDISNRIEMDVLNTT
                     KELLRADLRLKKSSYLPSLAAFGSYSVSASRNEFDIFENKPWFRTSIIGLKLNVPIFD
                     GFQKAARVNQARFALNKMENQIQTATQGFQLEAQTAKTTLTNALGSLANQEKNRTLAA
                     DVARITKIKYQQGVGSSLEVMDAETALKVAETNYYAAMVDAFIAKVDFEKATGTLIK"
     gene            6455..7573
                     /locus_tag="PKU82_08245"
     CDS             6455..7573
                     /locus_tag="PKU82_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOZ82901.1"
                     /translation="MKKVIIAITAIGFLTACGGGNSSKKVEELEKLRKQQSELNEKIK
                     SLEAEIALTDTSRKDKIKFVAATDMSLKPFTHYIEVQANVEGDEDVVLSAKTPGTVTS
                     VNVKAGDHVTKGQVLATLDDQIMQKAIAEVQNELNLVTTLYNRQKNLWDQQIGSEVQY
                     LEAKTRKEGAEKKLASIQEQWDMTRIKAPFSGTVDNVMIKTGQLVSPQVPAIRVVNLE
                     MLKVKGELPETYIGRVNKNDNALVYFPDLGKEITSKVYYSGNAISNLNRTFNVEVRFG
                     SKENFLRPNMLAVLKIADYNKEKAMSLPVSVVQKGLDGEYVFVAENSGGKTVAKRKTV
                     KSGMTYNGNVEIVEGLNEGDKVITTGYHDLVEGDIIKL"
     gene            7588..>10195
                     /locus_tag="PKU82_08250"
     CDS             7588..>10195
                     /locus_tag="PKU82_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOZ82902.1"
                     /translation="MKDIFKEFKPSSWAIDNRTAIYIITVIITLTGIMSYINIPKESF
                     PDISLPNIYISVVYPGTSPKDMENLVVRHIEKECKGISGVKKIKSNALQDYCSIVVEF
                     NSDVKIEDAKRKVKDAVDRARNDLPQDLPNEPDIIEVNFSDLPIMAVNLSGDYDLAKL
                     KRYADEAKDKIESLKEVKEVKLIGELEREIQINVDMLKLQSSGLTMSDIERAVKYENM
                     TVPGGGVRMADGTRRSLSVSGEFTNVDQIKNLVVNSINGAPIYLKDIAEVVDSYKEQE
                     SYARLNHKNVITLSIIKRSGENLIDASDKIVSVVNELKAEAWPKDLNVVLTGDQSEQT
                     RTQLHDLINTIIIGFVLVLIVLMFFMGTSNAFFVAMSVPLSMCVAFLVLPVIGFTINF
                     IVLFSFLLALGIVVDDAIVVIENTHRIFDNGKMDIVRAAKMATGEVFLPVLSGTLTTL
                     APFVPLAFWKGIIGKFMFFLPITLIITLLASLVVAYIINPVFAVSFMKKHEEKHDPYK
                     GLKITSIIFIAIALLFYVTGAFGMGNFTIFILLLNLLHRFWLHKVIVRFQTKTWPAVQ
                     ESYKRMLIKVLHRPRTMMAGTVGLLFLSFIAIGLRNGGVNFFPIADPNFVYVFTTLPV
                     GTDQAYTDSVTKILEGKVYNVLGEKNDVVKSVIASVGVNVSDPSDEDQGYYANKSKIE
                     VAFVEYSKRHGVSTSNYLEKIREAVKNTVPGAEVTVAQEQGGPPVGKPISIEVSGDDL
                     QAITTTSKSLKRYLDSLQIEGVEELKSDLQDSKPQIVFDINREHMNRLGIMTGTVGNE
                     INMAVLGREASKYRDLNDEYKIMLRYKLDQRYDVSLLNNLKILYRDMSMGGLVRNVPL
                     SSFATVKYDNTYG"
BASE COUNT         3320 a         1719 c         2028 g         3128 t
ORIGIN      
        1 tttgcaagaa tctatggcta tggtgtggca ggagtaatat tctttcactt tatggataag
       61 ttgctcaggc catttggatt aaacggaagg tctgtaattc cattgatgag tggtgtggca
      121 tgtgccgtgc ctgcaatcct tgcaacacga accatacaaa gttggaagga gcgtattgta
      181 acaattatgg taacaccatt gatgagttgt tcggcacgac tacctgttta taccctgctc
      241 atttcattgg ttattccacg atattatgtt tttggattta tcaattctca ggggttggtg
      301 atgatgctca tgtatcttat cggttttgtt gcagctatat tttctgcatg gttgtttaag
      361 atgtttgtta aagccaagca gcgtgatttt tttatcatgg aattaccggt ttaccgtttg
      421 ccacgatgga agaatatctt ctttgtaatt ttcgataaag taaaaatatt tttattcgat
      481 gcaggaaaaa ttatcattgc catttctatt gtactttggt ttttatcatc tcgtggaccg
      541 ggaaaagatt atgccgaaac agaaaaacta tatgaagcaa aggcagaact tcctgaaaca
      601 acacaggacg aactgaacca attacaggca cagaagctgg aacattccta tgcaggatgg
      661 acaggaaaaa ttattgaacc tgcaattaaa cctttgggct tcgactggaa aattggaatt
      721 gccttggtga catcatttgc agctcgcgaa gtttttgttg gaaccatgtc aaccatttac
      781 agtgtaggta gtgaaggtga aaatttacca atcagagaaa agatgatggc agaaaaacga
      841 cctgatggaa cacctcttta tacattagct gttggcattt cactgatgtt gttttatgca
      901 tttgccatgc aatgtatgag tactcttgct gttgttaaac gagaaaccgg cacatggaaa
      961 tggccaatca ttcagtttgt gtatatgagt gctttagcat gggtatcaag ttttgctgct
     1021 tttcagctac tgagttaatt tattattttt cgaccgaaat taattatggc aaaagttact
     1081 ctcgagttta tagaagaagc attaaagaaa tatgttccgc atcaatctat cagatattgc
     1141 atacaatgga ttgaagaaca tcagatcaat cttaaaatta caagaagccg gaatagcaaa
     1201 tatggtgact atcgtccgcc acaaagaggc ttcggccatc gtatttctat caatcacgat
     1261 cttaatcctt atgcttttct catcacattc attcatgaag tagcacatct aaatcagtgg
     1321 aaacgcaaga aacaaattat tattccacac ggaaaagact ggaaagaaga gtatcgtaaa
     1381 ctgatgattc ccattctacg cgagaatatt ttcccgcccg atgttgttgc tgcactgaat
     1441 aattatatga caaatccatc agctacaagt tgctcagacc atgacttgct tagggcattg
     1501 cgcaattatg acagagaaga agataaatgg attacactgg aagaactgaa ttttggtgtc
     1561 aagtttaaaa taagaacagg ccggactttt attaagaaag aaaaactacg taaaaattat
     1621 agttgtattg attgtcgttc taaaagtatt tacttcatca atcccataac ggaagtgatg
     1681 cctgtagatc attgaacaaa gaaacgatca ggaacattaa taagataaac ttttttgcgt
     1741 gcacgtgtta ttgctgtata tagccatcgc atataatcaa caccatacat ttcttctgtc
     1801 ataaagcccg gatcaataaa cactgtttcc cattgtccgc cttgtgcttt gtgacctgta
     1861 atagcatagg catattttac ctgcaatgca ttgagataag gatcttctcg tagtttacgc
     1921 atgtttactt tgccatctgt tttgcgataa tgatttctca cttcatcaaa caatttttta
     1981 tactcatcat aagttaatgc aggcgactcg ctgtttaatg tatcaagcaa aagttttact
     2041 tcaagccttg ttgatttttc atcagataat tctattgtag cattggcaaa acgaaaccca
     2101 aaatactctt cctgcttttt taaacgaatc agtttaattt catcgccatt ggcaataaat
     2161 gcattgtcat tgtctgattc atgccagtag taattatttt ttacaaccat cagcctttcg
     2221 tttttctcta ctacctcttc aaagccataa aaagtttttc tgataaattc attgtatcgg
     2281 tttgcctgct tattacttct gcacaacaca gttactgtgt tatcatcacc ggaagggaaa
     2341 tatcccgaaa gtgcttcaga gagctctgct gaagttactt ggtttacatc atcaaatcct
     2401 gatgttttta ggcgaggaaa atgctcttct ctttcaagca ataatcgtgt gcaatgtgca
     2461 ttagctaata ttccggagtg aagttgttgc cgcaccactt ctttcaattc atattgcctt
     2521 acctgaagct gacagttgtt ttgaagaaaa ttctgatcca atgcaggact gcattgcaaa
     2581 ccaacggggg gcaattgtgc agtgtcgcca ataaatacta tagcattgtc tgcctgactg
     2641 taaacatgat ctactaagtc agaaagtaaa cttgctcctt taaacaatgt cttatcatct
     2701 tcattcgaaa tcattgaaga ctcatcaata ataaaaatct gctttttaaa tttgtttggc
     2761 tttacctttg ttttgatgtc gccattttta tcacttttta tttcatacaa gtaccgatga
     2821 attgtagaag catcatcacc ggtataatgg ttaatcactt tagctgctct gcctgtcggg
     2881 gcaagcatta tcactctgaa atcgtaatga taaagtgttt ttatcattgc attaattacc
     2941 gttgtttttc ctgttccggc atagcctttt aaaagaaagc acttatatgc agtattttct
     3001 gtaataaact ccgatagtcc tgcaaaagca tgcttctgtg cattggtagg ttcaaatgga
     3061 aaattcttca gtaagagatt aaaaactgca gcaggcttca atgtttatct tgcattatta
     3121 tcggataaaa atacaaaagg ggaagttaac atcataagat agataaccaa tgataataac
     3181 aatgatgtaa agagttgtta ttactaaaca aacatttaat tagtagaata atattttgaa
     3241 tcccacactc tgaattttta attatcacct cattaatctt tgtactttcg tgcattctta
     3301 aaattgtaat acatgctcag catagacatt cacacacata tactaccgga gaaattaccg
     3361 gattggaagt cgaaatttgg ctatggtggt tttatcacat tagatcatca caaaaattgt
     3421 tgtgccagga tgttgcgtga tgacggcact ttttttcgtg agattgaaag taattgctgg
     3481 gatgcaaaga caagaatgaa tgactgcgat gtacatggtg ttcatgttca ggcattgagt
     3541 actgtacctg tcatgttttc atattggaca aaacctgcag atggacttgc tgtatcagaa
     3601 tttttaaatg atcatattgc aacaattgtt caacaatacc caaaacgttt tgtaggctta
     3661 ggtactgttc ctctgcaagc accggattta gctataaagg agatggaaag gtgtaagaaa
     3721 attggactgc aaggcttaca aataggctcg catgtaaacg actggaatct taatgatgca
     3781 aacctgtttc cgttttttca ggcatgtgca gaaaacaaca tgccgctttt tgttcaccca
     3841 tgggaaatga tgggtcaaaa agatatgcaa cgttactggc tgccttggtt ggtgggaatg
     3901 ccggcagaaa cttctcgtgc tatttgttca atgatttttg gaggtgtgtt tgaaagattg
     3961 ccaaacttgc gtgttgcttt tgctcatggc ggaggaagtt ttccgtcaac attcggacgc
     4021 attaaacatg gttttgattg ccgccccgat ttggttgcaa tagataatgc atcttcaccg
     4081 gagagatata tcggtaagtt ttattttgac tcattggtac atgatgaaaa catgttgcat
     4141 tacctgatta atttcacagg agccaatcgt attgcattag gaagcgacta tccttttcct
     4201 ttaggtgaaa atattcccgg aaccctgatt aaaaatgcaa aactcgattt agaaactaaa
     4261 gaacgattgc ttggtggcac agcattagag tggttgggtt tatcaaaaga aatgtttctg
     4321 agatagattt tctactttca ttcaatctcc caaaacaatg ttaaaattgt taaagacttc
     4381 ttaaattttt tctcggaaac ttaaaaaact aaaatagttt ccatagtttt gcccagtttt
     4441 taacaattat catggatata agagaaagaa ttttagagtc ggggttggag ttgtttctgg
     4501 cacatggtgt caggcgtata accatggacg acatagcaag acatttgtcc gtttctaaaa
     4561 aaacaattta tgcctgcttt gatgataagg atgaaattgt atctgaatgt tgtcagggat
     4621 atttaaaaaa cagccgttgc gagtttgaac atgttgaaac agaaagcaag gatgccattc
     4681 acgaaatatt tctgacaatg gaaaaattgc aagcgatgtt caccaccatc aacccagcat
     4741 tgtttgaaga cttacaaaaa ttttatccgc gagtgtggag tatgtatgtt cagtttaaag
     4801 ataaacatgt tgcagaagtc attgaaagaa atcttttaaa aggcattgag caaaaacttt
     4861 accgtccgga tatcaatatt aaaatattgg taaaactgag aatagaagaa attactctgg
     4921 gaatgaatac tacagtgttt cctttaaaga actttaaaac gcaggatgtt caggttgcat
     4981 tgctcgatca ttatctgcat ggcattgtaa ccgttaaagg acataaactc atcaataaat
     5041 ataaaaacat aacagacgaa gaataaataa caaccgtatg ctgaatcaaa ttaaatttct
     5101 tacaggttta ctgttgctga tatcaatcaa tatacaggcg cagcaatccg ataaaaagtc
     5161 ttattccttt tcattgaaac aggcaattga ttttgctttt caaaatcaga ccgatattca
     5221 aaatgcagtt ctcgatcaga agtcagcaca aagcagagct aatgaattat tgggaattgg
     5281 tttaccgcaa atcagtgcca gtgccgacat cacacatttt attgacatac caacaacctt
     5341 tgtgcctgca gaattttttg gtggcgagaa aggtagctat gcccccgtac aatttggcca
     5401 gccatatagc tctactgccg gtttatctgc atcacaacta ttgtttgacg gatcttattt
     5461 agttggcgta aaagcatcac gcatgtatgc cgatttgatg cgtaagcaag ttattcagac
     5521 taaaactgaa aaagctgcac tcatcagcaa ggcatattat aacgtgcaag ttaacagtga
     5581 gcgtttgcag atgctcgatg caaatgttaa gcgattaaaa aagcagcttg atgaaatgca
     5641 ggtaatgtac aataatggtt ttatcgaaaa gattgattta gaccgaatca gtcttgccta
     5701 taacaatatt gaaaccgaaa aatcaaatgc ggacagactg attgtgcttg cacaaaacct
     5761 gttgaaattt cagatgggat tgaatgttaa tgatgagttg acactcacag ataaattgga
     5821 agacataaaa gttgttgccg acaataacct gaatcagaac attgatataa gcaacagaat
     5881 agaaatggat gttttgaata caaccaaaga attactcaga gctgatttaa gattaaaaaa
     5941 atcttcttac cttccttcac ttgcagcctt tggctcgtat agtgtaagtg catcgcgcaa
     6001 tgaatttgat atttttgaaa acaaaccctg gtttcgtact tcaataattg gcttgaaact
     6061 taacgtaccc atttttgatg gctttcagaa ggcagcacga gttaatcagg caagatttgc
     6121 gcttaataaa atggagaatc aaattcagac tgcaacacaa ggctttcaat tagaagcgca
     6181 gactgcaaag acaactctaa ccaatgcatt aggctctctt gccaatcagg aaaaaaacag
     6241 aacattagct gctgatgtag caagaattac aaaaataaaa tatcagcaag gtgttggcag
     6301 tagccttgaa gtaatggatg cggaaacagc tttgaaagta gcagaaacaa attattatgc
     6361 tgcaatggta gatgcattta ttgccaaagt agattttgag aaagcaacag gaacattaat
     6421 aaaataaaat ataaactctg acataaataa atatatgaaa aaggtaatta tagcaataac
     6481 agcaattgga tttcttactg cctgtggtgg aggaaacagc agtaaaaagg ttgaggaact
     6541 tgaaaaactc agaaagcaac agtctgagtt aaatgaaaag ataaaatctt tagaagcaga
     6601 aattgcactc acagatactt ccagaaaaga caaaataaaa tttgttgcag ccacagatat
     6661 gagtttgaaa ccatttactc attatattga agtgcaggca aatgttgaag gtgatgaaga
     6721 tgttgtattg agtgcaaaaa ctcccggaac tgttacctct gtaaatgtaa aagctggcga
     6781 ccatgtaacc aaaggtcagg tgttggcaac gctcgatgat cagattatgc agaaggcaat
     6841 tgcagaagta cagaatgaac ttaatctggt gacaacactt tataaccgtc agaaaaatct
     6901 gtgggaccaa cagataggta gtgaagtaca atatctggaa gcaaaaacaa gaaaagaagg
     6961 tgcagagaaa aaacttgcat ctattcagga gcaatgggat atgactcgta ttaaagcacc
     7021 attcagcgga acagtagata atgtgatgat aaagacagga cagttggttt caccacaggt
     7081 tcctgcaata agagttgtta atctcgaaat gcttaaagta aaaggcgagt tacctgaaac
     7141 ttatatcgga agagtaaata aaaatgataa tgcattggtt tattttcctg acttaggaaa
     7201 agaaattaca agtaaggttt attattccgg aaatgccatc agcaacctga accgtacatt
     7261 taatgttgag gttcgttttg gcagtaaaga aaatttcctg cgacctaata tgcttgccgt
     7321 tttaaaaata gctgattaca ataaagagaa agcaatgtca cttcctgtta gtgttgtaca
     7381 aaaaggactt gatggtgagt atgtttttgt tgctgaaaat tcaggaggca aaactgttgc
     7441 aaagcgtaaa acagtaaaaa gcggaatgac atataatggc aatgttgaaa ttgttgaagg
     7501 attgaatgaa ggtgataaag tgattacaac aggttatcac gatttggttg aaggcgatat
     7561 cattaaacta taaaccgcaa gcattttatg aaagatatat ttaaagaatt caagccctca
     7621 tcatgggcaa tagataaccg tacggctata tacattatta ccgtcatcat tacattgacg
     7681 ggtataatgt catacatcaa tattccgaag gaaagttttc cagacatttc tttaccaaat
     7741 atttatatca gtgttgttta tccgggcact tcaccaaagg atatggagaa cttagtggtg
     7801 cgccacattg aaaaggagtg taaaggaatt tcaggtgtaa aaaaaatcaa gagtaatgca
     7861 ctgcaggatt attgtagtat agtggtagaa tttaacagcg atgtaaagat tgaagatgca
     7921 aagcgaaaag ttaaagatgc tgttgaccgt gcacgtaatg atctcccgca ggatttaccc
     7981 aacgagcctg atattataga agtcaacttt tctgatttgc ctattatggc tgtaaacctt
     8041 tccggtgatt atgatcttgc aaaattaaag cgctatgccg atgaagctaa ggataaaata
     8101 gaaagcctta aagaagttaa agaagttaaa ctgattggag agttagagcg tgagattcag
     8161 ataaatgttg acatgcttaa gctgcaatca tcaggattaa cgatgagtga tattgaacgt
     8221 gcagttaagt acgaaaatat gactgtgccc ggtggcggtg tgcgcatggc cgatggtaca
     8281 cgcaggtcgt tgagtgtttc gggtgagttt accaatgttg atcagataaa aaacttagtt
     8341 gtaaattcta ttaatggtgc acctatttat ttaaaagata ttgcagaagt agttgactcc
     8401 tacaaagagc aggaaagcta tgcacgtcta aatcataaaa atgtaattac actaagcatt
     8461 atcaaacgct ccggtgaaaa cctgattgat gcatcagata aaattgtttc tgttgttaat
     8521 gaacttaaag cagaagcctg gccaaaagat ttgaatgttg tacttacagg tgatcagagt
     8581 gaacaaacac gtacgcagtt gcacgacctg attaatacca tcatcatcgg ttttgtattg
     8641 gtattaattg tacttatgtt ttttatggga acaagcaatg cattctttgt tgccatgtct
     8701 gtgccactat ctatgtgtgt ggcatttttg gttttacctg ttataggatt tacaattaac
     8761 ttcattgtat tattctcttt tctgcttgca ttaggaattg ttgttgacga tgcaattgtt
     8821 gttattgaaa acacgcatcg tattttcgat aatgggaaaa tggatattgt tagagctgca
     8881 aaaatggcaa caggcgaagt tttcttacct gtgctttccg gaacactcac cacacttgca
     8941 ccttttgttc cattagcatt ctggaaaggc atcattggta agtttatgtt ctttcttccc
     9001 attaccctta tcataacatt actggcatca ttggtggttg catacatcat caacccagtg
     9061 tttgctgtta gttttatgaa aaagcacgaa gaaaagcatg acccatacaa aggtttaaaa
     9121 attacttcaa taatttttat tgcaattgca ctgttgtttt atgtgacagg tgcatttggc
     9181 atgggtaact ttacaatctt cattctgttg ttaaatttac ttcaccgttt ttggttgcat
     9241 aaagttattg tacgttttca gacaaaaaca tggcctgcag tacaggagtc atataaaaga
     9301 atgttgatta aagttcttca ccgcccacgc actatgatgg caggaacagt aggattgttg
     9361 tttttatcat tcattgcaat tggtcttagg aatgggggag taaatttttt cccaattgca
     9421 gatcccaact ttgtttatgt gttcacaaca ttgcctgttg gaaccgatca ggcctataca
     9481 gactctgtaa caaaaattct tgaaggtaaa gtttataatg tgttgggcga aaagaatgat
     9541 gttgtaaaat ctgttattgc cagcgttggt gtaaacgttt ctgacccatc agacgaagat
     9601 cagggatact atgccaacaa gagtaaaata gaagtagcat ttgttgagta cagtaaaaga
     9661 catggtgttt caactagtaa ttatttagag aaaatcagag aagcagtaaa aaatactgtt
     9721 cccggtgctg aagttactgt tgcacaagag caaggcggac cacctgtagg caaacctatt
     9781 tctattgaag tgagtggtga tgatcttcag gcaataacta caacttctaa aagcttgaaa
     9841 cgatatcttg attccttgca gattgaaggt gtagaagaat taaaatcaga cctgcaggat
     9901 agcaaaccac aaatagtgtt tgacatcaat cgcgaacaca tgaaccgtct tggaattatg
     9961 acaggtaccg taggaaatga aatcaatatg gctgtacttg gtcgtgaggc ttcaaagtac
    10021 cgcgatttaa atgatgagta taaaattatg ttgcgttata aactggatca gcgatatgat
    10081 gttagtctgc tcaacaattt aaaaatattg tatcgtgata tgtcaatggg aggacttgtg
    10141 cgcaatgtac cgttatcatc ttttgcaacg gtgaaatatg ataacaccta tggtg
//
LOCUS       DAOOOL010000092        10152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_612_length_10152_cov_102.944637, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000092 DAOOOL010000000
VERSION     DAOOOL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_612_length_10152_cov_102.944637"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /gene="pyrH"
                     /locus_tag="PKU82_08255"
     CDS             complement(<1..99)
                     /gene="pyrH"
                     /locus_tag="PKU82_08255"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611227.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOZ82903.1"
                     /translation="MKYKRILLKLSGEALMGEKNFGIDNNRLTQYAE"
     gene            complement(136..1041)
                     /gene="deoC"
                     /locus_tag="PKU82_08260"
     CDS             complement(136..1041)
                     /gene="deoC"
                     /locus_tag="PKU82_08260"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOZ82904.1"
                     /translation="MAKADLRFLNHLPPVDQVGVEERVARLNKRSIKNESKMEALKLA
                     ISMMDLTTLDAKDTPGKVRQLCAKAIRPHEMEGIPSAAAVCVYANLVSVAKQALEGTK
                     VKVAAVSTAFPSGMSNRKFKLDETRYAVAEGADEIDMVISRGEFLRGEYNFVYDEIAA
                     VKEACGNAHLKVILETGELSTLDNVRKASELSIAAGADFIKTSTGKVQPAATLPVTLV
                     MLECIRDYYYRTGIKIGMKPAGGIATAKLALQYLVMVRETLGQEWLNADMFRFGASSL
                     ANDLLMQIIKQLTGAYQSADYFSKD"
     gene            1182..2528
                     /gene="purB"
                     /locus_tag="PKU82_08265"
     CDS             1182..2528
                     /gene="purB"
                     /locus_tag="PKU82_08265"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOZ82905.1"
                     /translation="MSDALFAVSPVDGRYAAVCSPLKEYFSEYALIRERVKVEVKYLI
                     ALAQLQLPQLHIDSGKFEALRNIYRQFTPADAAQIKQTEKTTNHDVKAVEYFVKEKLE
                     TLGLNNAKEFVHFGLTSQDINNTATPMLVRHGIAEVMLPALTDVLNKIESQAQEWNNI
                     SLLAHTHGQPASPTRLGKEMKVFSERLRSQLDMLKSIPHSAKFGGATGNFNAHAVAYP
                     AVDWISFADDFVNSLGLTRTTFTTQIEPYDNLSALFDNLKRINNILLDLCRDVWSYIS
                     MNYFSQTVKAGEIGSSAMPHKVNPIDFENAEGNLGIANALFEHLSAKLPISRLQRDLT
                     DSTVLRNTGVPFAHTLIALKSLLKGLNKISPNTQVISADLNRHAVVIAEGIQTILRRE
                     GYPNPYEALKDLTRGKENITLQDIHQFINTLQVGDTVKAELLKITPENYTGVNWSD"
     gene            complement(2520..3116)
                     /locus_tag="PKU82_08270"
     CDS             complement(2520..3116)
                     /locus_tag="PKU82_08270"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOZ82906.1"
                     /translation="MFTGIIETLGIVKKIENEGSNRHFTVSCSFADELKIDQSVAHNG
                     VCLTVVNINHENKTYKVTAVDETLRKTNLSSLVEGNEVNLERGMLLNTRLDGHIVQGH
                     VDGTAVCTSITDENGSWKFIFENDGSARNLIVEKGSVTVNGVSLTVVDAGDYMFSVAI
                     IPFTMQHTTFKNLKRGQTVNVEFDIIGKYVARLTEKSV"
     gene            complement(3116..5665)
                     /locus_tag="PKU82_08275"
     CDS             complement(3116..5665)
                     /locus_tag="PKU82_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ82907.1"
                     /translation="MEAWATHNRAGEITFNQISLLTYRVQIVTYTKTSSPADRPMLEM
                     NWGDGTQDSLPRISKTTVGIDISRNYYEGVHTFPGPAVYTVFFEDPNRNGGVVNIPGS
                     VNIPFYVESQIVINPVLGYNNSPVLLQPPIDNGAVGKIFIHNANAYDPDGDSLSYELI
                     KCKGINGLDIPGYFYPAASTSFTLDAVTGDLIWNTPTIQGEFNVAFLIREWRNGVNIG
                     YVERDMQIDIVVTNDNPPVINALNDICVTAGTLISQNVTATDPDAGNIITLSATGSPL
                     DTNFTPISPAVFPVVTDTNSVTGTFVWQTECVHVRKAPYQVLFKAQDNVNQINLVDLK
                     RMNITVVAPAPQNLTATPVQNSIHLTWDQSVCFNAEGYRIYRRIGFFGYVPSLCQTGV
                     PAFTGYTYLATVQGLANNTFVDDNNGAGLIPGNDYCYMVIAYFNDGAESYASNEACAK
                     IVQSLPIMTNVSVTATDATNGTMYVAWSKPKEIDSIQTPGPFEYRLLRGQGFLPSAFT
                     TVSTFSNLNDTTFNDVSLNTADNAWTYKVEFHNLTQGNTFKIGESLAASSVYLTTTPT
                     DNAIQLSWQASVPWTNSATVVYRKNGGGIYAVLDTVSGNTFNDTGLANDSLFCYKVET
                     IGGYSSPGFVSPILNFSQENCNAAKDNVNPCNISSATVSGSCLNSEVVLTWQKPDSAC
                     GDDVITYELYYAPSKSATPYLIVSLDGINQSTYTRNIPDSTSGCYYFMTVDSAGNKSS
                     LSEPYCAETCPVYTLPNVFTPDNDGKNDLFVPFPYRYVDHVELTIYNRWGNKVFETHD
                     KDILWKGTKDNGSTRLSDGVYYYIGKVYEIFIDGIKPRTLKGAVNLIGGAK"
     gene            complement(5715..6860)
                     /gene="mqnC"
                     /locus_tag="PKU82_08280"
     CDS             complement(5715..6860)
                     /gene="mqnC"
                     /locus_tag="PKU82_08280"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOZ82908.1"
                     /translation="MNVENLLSRALDFEFLNVEEGIHLFHHAPLTDLMFVANELRKKQ
                     KPENITASGEELVTWMIDRNVNTTNVCIANCKFCNFFRVPGHKEAYITSIEEYKVKIE
                     EMFRYGGDQLLLQGGHHPELGLSFYTGIFSELKKLYPQVKLHALGPPEVAHITKLEGM
                     THTEVLKALKDSGLDTLPGAGAEILSDRVRRMISKGKCTGREWLDVMRAAHQLNITTS
                     ATMMFGHIETLQERFEHLVMLRQVQSEKPAHAKGFLAFIAWPFQDENTILKRVKGIRN
                     KVTSEEFIRMIALSRIMLPNIKNIQASWLTVGKQTAQVALHAGANDFGSIMIEENVVS
                     VAGAPHRFTSQGIQDAIREAGFIPQLRSQQYQFREIPEMMEQQVINY"
     gene            7015..8439
                     /locus_tag="PKU82_08285"
     CDS             7015..8439
                     /locus_tag="PKU82_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HOZ82909.1"
                     /translation="MIKNKLADHNHDYSLVILTTSATKFDETVFSADELKYMKNELKD
                     KKNLVAINQYNRMVYVCLLENKAKNQSQYMESCRKAGHQVTGLLNKIKANSVLIEAKG
                     LRQEALCFAEGLALANYQFRKYKTSGDSNTLDVIALHSNKLTDKDLLWLNTMVDATCK
                     ARDLVNEPVSYLTATQLSDELVKLGKQAGIKVTVLNKKEIEKTGMGGLLAVNKGSQDP
                     PTFTIMEYKPAGHKNKKPFVLVGKGVVYDTGGLSLKPTANSMDYMKCDMGGAAAVGCA
                     IYALAKAKIPLHVVALIPATDNRPGENAYVPGDVIKMYSGKTVEVLNTDAEGRMILAD
                     ALHHAKKYNPALVMEFSTLTGSASSALGKYACAFLTTADQNQNSALKKSAEHVFERVV
                     EFPMWDEYGELIKSDVADIKNVGGPAAGMITAAKFLEHFIDYPYMHFDIAGTAFVHAE
                     DSYRGKGGTGFGVRLISEFLKNKI"
     gene            8442..9512
                     /gene="pdxA"
                     /locus_tag="PKU82_08290"
     CDS             8442..9512
                     /gene="pdxA"
                     /locus_tag="PKU82_08290"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOZ82910.1"
                     /translation="MSTITESEIKPKIGITIGDLNGVGIEVIIKTFADSRMLSVCTPV
                     IYGSSKVISYHRKSLHQQEFNFNTIRTAEEASHKKTNVINCWEEDVKVELGKPSTIAG
                     TYAFKSLEAAGKDVLDKKIDAIVTAPIDKSTIHSDAFPFKGHTEYFAKLCGNAEHVML
                     MVSDKLRVGLITGHVALKDVPAQLTKEKIIAKTKLVHQSLRSDFGLQKPRIAILALNP
                     HAGDNGLMGKEEQEIITPAISEMYNAGIGAFGPYPADGFFGSSRFKSFDAIMAIYHDQ
                     GLVPFKALAFSTGVNYTAGLPIIRTSPDHGTAYDLAGKNQASEDSFREAVYLACDIAK
                     QRQITSEITANPLKFSRMSADR"
     gene            9775..>10152
                     /locus_tag="PKU82_08295"
     CDS             9775..>10152
                     /locus_tag="PKU82_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82911.1"
                     /translation="MKNLIAIIFCLLIFETVNAQWTQTNGPEGGDISSIVTDGTNLYA
                     GTYYGGVYRSSNNGQMWTAINNGLTHIRVGTLWLNGADLFAVTDGGLYVTSNNGNSWS
                     LINIVLPPFTSVTTFAANATNLFI"
BASE COUNT         3128 a         2102 c         1863 g         3059 t
ORIGIN      
        1 ctctgcatat tgggtaagcc tattgttgtc tatcccaaaa tttttctctc ccattaatgc
       61 ttcaccgcta agtttcagta atattctttt atacttcata tcagatttgt gtttttatct
      121 ttttagtttt ttcttttaat cttttgaaaa ataatcggca ctttgatagg ctcctgtaag
      181 ctgctttatt atctgcatca gtaaatcgtt ggctaacgag cttgcaccaa acctgaacat
      241 atcggcattc aaccactcct gccctaatgt ttctctaacc atcactagat attgtaatgc
      301 caactttgca gtagcaatgc ctcctgcagg tttcatcccg atttttattc ctgttctata
      361 gtaataatca cgaatacatt caagcatcac caatgttacc ggcaatgttg ctgcaggctg
      421 caccttgcct gttgatgttt ttataaaatc tgcacctgcc gcaatagata actcacttgc
      481 ttttctcaca ttatccaatg ttgaaagttc tcctgtttcc aaaatcactt tcagatgtgc
      541 attgccacag gcttctttta cagcagcaat ttcatcataa acaaaattat attctcctct
      601 taaaaattca ccacgcgaaa taaccatgtc aatttcatcg gcaccttctg caacagcata
      661 gcgtgtttca tcaagtttaa attttctatt gctcatgccg ctgggaaatg cagtggacac
      721 tgctgccact tttacttttg ttccctccaa tgcctgcttg gcaacagaaa ccaaattagc
      781 ataaacacat acagcggcag cagaaggaat tccttccatc tcatgtggtc tgatggcttt
      841 ggcacaaagc tgtctcactt ttcctggtgt gtcttttgca tcgagtgtgg tgaggtccat
      901 catcgaaatg gctaacttca acgcttccat cttcgactca ttttttatgc tgcgcttgtt
      961 taatcgtgca acacgctctt ctacaccaac ctgatctaca ggtggcaggt ggttgagaaa
     1021 acgtaaatct gcttttgcca tgctgataag ataaaatttc agagagctaa aataaacatt
     1081 ccatatcaat tgcagtaaaa tttattgtcg taggatataa tccaaatcat aaacagcatt
     1141 taatttttaa aatctacctt tgcagcacca aatattatgc tatgtctgat gctctttttg
     1201 ccgtttcgcc tgttgacgga cgctatgccg ctgtttgtag tccgttaaaa gaatattttt
     1261 ctgaatatgc gctaatccgt gagcgtgtta aagtagaagt taagtatctt attgcactgg
     1321 cacaactgca attgccacaa cttcatattg attccggaaa atttgaggca cttcgcaaca
     1381 tctaccgtca gtttacaccg gccgatgcag cacagattaa gcagacagaa aaaacaacca
     1441 atcatgatgt aaaagctgtt gagtattttg taaaagaaaa attagaaaca ttaggcctga
     1501 acaatgccaa agagtttgtt cacttcggac ttacctcgca agacatcaac aacactgcaa
     1561 cacccatgct tgtccgtcat ggtattgccg aagtaatgct gcctgcatta acagatgtgt
     1621 taaacaaaat tgaaagtcag gcgcaggaat ggaacaacat cagcctgctg gcacatacac
     1681 acggacagcc agcaagccca acaagactgg gaaaagaaat gaaagttttc agtgaaagac
     1741 ttcgctcaca gttagacatg ctgaagtcaa ttccgcatag tgcaaaattc ggaggggcta
     1801 caggaaactt caatgctcat gcagtggctt accctgctgt tgactggatt tcttttgctg
     1861 atgattttgt gaactcactc ggattaacac gcacaacatt cacaacacag attgaacctt
     1921 atgacaacct ttcggcttta ttcgataatc taaaacgcat caataatatt ttattggatt
     1981 tatgtcgtga tgtttggagt tacatcagca tgaattattt ttcgcaaact gtcaaagccg
     2041 gtgagattgg ttcttctgca atgccacata aagttaatcc catagacttt gaaaatgcag
     2101 aaggtaatct tggcattgcc aatgcacttt tcgaacatct gtcagccaaa cttcctattt
     2161 caagacttca acgtgatttg actgatagca ctgtgttgag aaataccggt gtgccgtttg
     2221 cacatacact cattgcactg aaatctttac tgaaaggatt aaataaaatt tcgccaaaca
     2281 ctcaggtaat ttctgctgac ctaaatcgtc atgcagtagt aattgcagaa ggcatacaga
     2341 caattctcag acgcgaaggc taccctaatc cttatgaagc attaaaagat ttaacacgag
     2401 gaaaagagaa tatcactttg caggatattc atcagttcat caacacactt caggttggtg
     2461 atacagtaaa agctgaattg ctgaaaatta ctcctgaaaa ttataccggt gtaaattggt
     2521 cagactgatt tttccgtcag ccttgcaaca tattttccga taatatcaaa ctcaacattt
     2581 acggtttgtc cgcgtttaag atttttaaat gtggtatgct gcatggtgaa tggaattatt
     2641 gcaactgaaa acatataatc accggcatca actactgtaa ggcttacacc gttgacagtt
     2701 acagagcctt tctcaactat taaatttcgt gctgagccat cattttcaaa aataaatttc
     2761 cagcttccat tttcatctgt tatagaagtg caaacagcag taccgtcaac atgcccctgt
     2821 acaatatgtc cgtctaaacg tgtattcaac aacatgcctc tttcaagatt tacttcattg
     2881 ccttcaacca aactactaag gtttgttttg cgcaatgttt catcaacagc agtaacctta
     2941 taggttttgt tttcatgatt aatatttaca acagttaagc aaacaccatt gtgtgcaaca
     3001 ctctgatcta tttttaattc atcagcaaaa gaacatgaaa cagtaaaatg acgattactg
     3061 ccttcatttt ctattttctt tacaatgcct aaagtttcaa taattcctgt aaacattatt
     3121 tagcacctcc aatcaggtta acagcacctt tgagggtacg tggttttatt ccatcaatga
     3181 aaatttcata cacctttcca atgtaataat ataccccatc actcagtctt gttgagccat
     3241 tatcttttgt tcctttccat aaaatatcct tgtcatgtgt ttcaaaaact ttatttcccc
     3301 aacggttgta gatggtgagt tcaacatggt caacataacg ataaggaaat ggaacaaaca
     3361 aatcgttctt tccatcattg tcaggtgtaa acacattagg caatgtataa acaggacagg
     3421 tctctgcaca ataaggttcg ctaaggctgc ttttatttcc tgcggaatca actgtcataa
     3481 aataataaca accacttgtg ctgtcgggaa tattacgtgt gtatgtactt tgattaattc
     3541 catccaaaga gacaatcaga tatggtgtag cactctttga aggcgcgtaa tacaattcat
     3601 aagttatcac atcatcacca caggcgctat caggtttttg ccacgttaaa acaacttcgc
     3661 tgttcaggca tgaacccgat actgttgcac tgctgatatt gcatgggtta acattatcct
     3721 tagctgcgtt acaattctcc tgcgaaaaat tcagaatagg agaaacaaat ccgggtgatg
     3781 aatagcctcc tattgtttca accttataac agaataaaga atcgtttgct aagcctgtat
     3841 cgttaaatgt atttcctgaa acagtatcta aaactgcgta aattccacca ccgttttttc
     3901 tgtaaacaac agttgcactg tttgtccaag gtacgcttgc ctgccacgac aattgaatag
     3961 cattgtctgt tggtgtggta gtcagataaa cacttgaagc agcaagcgac tctcctatct
     4021 taaatgtatt tccctgtgta aggttatgaa attctacttt gtaagtccat gcattgtcag
     4081 cagtattcaa cgatacatcg ttaaaggtag tatcattcag attgctgaat gttgaaactg
     4141 ttgtaaacgc tgatggtaaa aagccctgtc cacgcaacaa tcggtattca aaaggtccgg
     4201 gagtttgtat gctgtcaatt tctttaggct tagaccatgc cacatacatg gttccattgg
     4261 tagcatcagt agcagttaca ctaacattgg tcataatggg tagtgattga acaattttag
     4321 cgcaggcttc gttagaggca taactctctg ctccatcatt aaaataagca atcaccatgt
     4381 agcagtaatc gtttccggga atcagccctg caccgttatt atcatcaaca aaagtgttat
     4441 tcgccaatcc ctgaaccgtt gccagataag tatatcctgt aaaagccgga acgcccgtct
     4501 gacaaagcga aggcacatat ccaaaaaatc caattcgtct gtaaattcga tacccttctg
     4561 cattaaaaca caccgactga tcccaagtaa gatgaataga attctgcaca ggtgtagcag
     4621 taaggttctg tggtgcaggt gcaacaacgg taatgttcat tcttttcagg tcaacaagat
     4681 ttatctgatt gacattatcc tgtgctttaa acaaaacctg atagggtgct ttgcgaacat
     4741 gtacacactc tgtctgccaa acaaaagtgc cggtaacaga attggtgtcc gttacaacag
     4801 gaaaaactgc aggacttatt ggtgtaaagt tggtgtctaa cggacttcct gttgccgata
     4861 aggtgataat atttcctgca tcaggatcgg ttgctgttac attctgagaa attaatgttc
     4921 cggcagtaac acaaatgtca ttcaatgcat ttatgaccgg aggattgtca tttgttacaa
     4981 caatatcaat ttgcatatcg cgctcaacat agccaatgtt tacaccattg cgccattcgc
     5041 gaattaagaa agccacatta aactcaccct gaatggttgg tgtattccaa attaaatctc
     5101 cggtaacagc atccaacgta aatgatgtgc ttgcagcagg ataaaaatat ccgggaatat
     5161 caagtccatt gatgccctta cattttatca gttcataaga taaactatca ccatcaggat
     5221 cataagcgtt ggcattatga ataaatattt ttccaacggc tccattatca attggcggct
     5281 gcaacagcac aggtgaatta ttatatccca gcacaggatt gatgacaatt tgcgactcta
     5341 cataaaatgg aatatttaca gagcccggaa tatttacaac accaccattg cggtttgggt
     5401 cttcaaaaaa cacagtataa actgcaggcc cgggaaaagt gtgaacacct tcataataat
     5461 ttctgctgat gtcaatgccc actgtagttt tacttatacg cggcaaagaa tcctgcgtgc
     5521 catcacccca attcatttca agcataggtc tgtctgcagg actgctcgtt ttggtataag
     5581 taacaatctg cactctgtaa gtaagcaatg aaatctgatt gaaagttatt tcacctgcac
     5641 gattgtgtgt tgcccatgct tccatttgca aaccgaaaag cagcaaaaaa aagattgaaa
     5701 tttttctcat acaatcagta gttgattacc tgttgttcca tcatttcagg tatttctctg
     5761 aactgatatt gttgtgacct caattgagga ataaatcctg cttcgcgaat agcatcctga
     5821 ataccttgtg atgtaaaacg atgtggtgca ccggcaacac tcactacatt ttcttcaatc
     5881 atgatagagc caaaatcgtt tgctcctgca tgcagggcca cttgtgccgt ttgctttcct
     5941 acagtgagcc atgatgcctg aatgtttttg atgttaggca acataattct gctcaatgca
     6001 atcattctga taaactcttc ggaagtaact ttgttgcgaa taccttttac tctctttaaa
     6061 attgtattct catcctgaaa tggccatgca ataaaggcaa gaaaaccttt tgcatgtgca
     6121 ggtttttctg actgcacctg tctgagcatg actaaatgtt cgaaacgctc ctgcaatgtt
     6181 tctatatgac caaacatcat tgttgcagat gtggtaatgt ttaactgatg tgcagcccgc
     6241 atcacatcaa gccattcacg acctgtacat tttcctttag aaatcatcct gcgtactctg
     6301 tcactcaaaa tttctgctcc tgcacccggc aaggtatcaa gtcctgaatc ttttaaggct
     6361 ttaaggactt cagtatgcgt cataccttct aacttagtga tatgtgccac ctccggtggg
     6421 ccaagtgcat gtagttttac ctgcggataa agtttcttta actcactgaa aataccggta
     6481 taaaatgaca aacctaattc cggatgatga cctccctgta gcaacaactg atcgccacca
     6541 taacgaaaca tctcttcaat cttaacctta tattcttcaa tgctggtgat gtatgcttct
     6601 ttatgccccg gcactctgaa aaaattacaa aacttacaat tggcaataca tacattggta
     6661 gtatttacat tgcggtcaat catccaggta acaagctctt ctccagaagc agtaatattt
     6721 tcaggcttct gcttttttct caactcattg gcaacaaaca tcaggtcggt taatggtgca
     6781 tgatgaaaaa ggtgtatccc ctcttcaaca ttcaaaaatt caaaatccag tgcacgactc
     6841 agtaaatttt ctacattcat actttaagaa ttcactttac tcaacagcaa acggtgtgat
     6901 ttgtttttat aatttcacgg ccgcaaacgt ctttcttttc aatgcgtaaa tttatagaaa
     6961 tttatggcag atgagaagtt gattctgtca ttttaattaa cattaattta aaatatgatt
     7021 aaaaataaac ttgcagacca taaccacgat tattccttag taatcctcac cacatcagct
     7081 acaaaatttg atgaaactgt cttttctgct gatgagttaa agtatatgaa aaacgagctt
     7141 aaggataaaa aaaatcttgt tgccattaat caatacaacc gcatggtcta tgtctgcctt
     7201 ctggaaaata aagctaagaa tcaatcgcag tatatggaat cgtgccgcaa ggcaggccat
     7261 caggtaacag gattgctgaa caagattaaa gctaatagtg ttttaattga agctaaaggc
     7321 ttgcgtcagg aggctttatg ttttgccgaa ggtttggctc ttgccaatta tcaattcaga
     7381 aaatataaaa cctctggcga cagcaataca cttgatgtga ttgccctgca ttcaaacaaa
     7441 ttaacagata aagatttatt gtggttaaac accatggtag atgctacctg caaagcccgt
     7501 gatttggtga atgagcctgt aagttattta actgcaacac aactatctga tgagttggta
     7561 aaattaggaa aacaagcagg cattaaagtt actgtgctca acaaaaaaga aattgaaaaa
     7621 acaggcatgg gtggattgct tgccgtaaac aaaggcagtc aggatcctcc cacttttact
     7681 attatggaat acaaacctgc aggacataaa aacaaaaaac catttgtatt agtcggcaaa
     7741 ggtgtagtgt atgatacagg aggattaagc ctgaagccta ctgctaacag catggactat
     7801 atgaaatgcg acatgggtgg agcagcagcc gtaggatgtg ccatctatgc attggcaaaa
     7861 gcaaaaattc ctttacatgt agtagcattg attcctgcta cggacaaccg ccccggagaa
     7921 aatgcttatg ttccaggtga tgtaataaaa atgtacagtg gcaaaactgt tgaagtttta
     7981 aataccgatg cagaaggcag aatgattctt gccgatgcac ttcatcatgc aaaaaaatac
     8041 aatcctgctt tagtcatgga gttttctaca ttaacaggat cggcatcaag tgctttagga
     8101 aaatatgcat gtgcattttt aacaacggca gaccaaaatc aaaattcagc attgaaaaaa
     8161 agtgcggaac atgtttttga gcgtgtggta gaatttccta tgtgggacga gtatggcgag
     8221 ctgataaaat ctgatgttgc cgatattaaa aacgttggtg gtcctgcagc aggaatgata
     8281 acagctgcaa aatttcttga acattttatt gattacccct atatgcactt cgatattgca
     8341 ggcactgcat ttgttcatgc cgaagattct taccgtggaa aaggtggaac tggatttggt
     8401 gtgcgactga tttctgaatt tctaaaaaat aaaatctgat tatgtcaact ataactgaaa
     8461 gtgaaataaa acctaaaata ggaatcacca ttggcgacct gaatggtgtt ggaatagaag
     8521 tcatcattaa aacttttgcc gacagcagaa tgctctctgt atgtacacct gtcatctatg
     8581 gttcttcaaa agttatttcc tatcatagaa aatcactgca tcagcaggaa tttaatttca
     8641 acaccatccg tactgccgaa gaagcaagtc acaaaaagac caatgtcatc aactgctggg
     8701 aagaagatgt taaagttgaa ttgggaaaac catcaaccat tgcgggaacc tatgcattca
     8761 aatcactcga agctgcaggc aaagatgtgc tcgataaaaa aattgatgcc atagttacag
     8821 cgcccattga caaaagcacc attcacagcg atgcatttcc tttcaaaggg catacagaat
     8881 attttgcaaa gctctgcggc aatgccgaac acgttatgct tatggtgagc gacaaacttc
     8941 gtgttggact catcacaggt cacgtagctt taaaggatgt gcctgcacag cttaccaaag
     9001 aaaaaattat tgcaaagacc aaattggtac atcagtcact tcgcagcgac tttggtttac
     9061 aaaagccacg tattgccatt cttgcactca acccacatgc tggcgataat ggtttaatgg
     9121 gtaaagaaga acaggaaatc atcacccctg ccatcagtga aatgtacaat gcaggcattg
     9181 gtgcctttgg accctaccct gccgatggtt tttttggatc ttcgcgcttt aaaagtttcg
     9241 atgccatcat ggccatctat cacgatcagg gattggtacc attcaaagca ttggcattct
     9301 caacaggggt caactacaca gcaggtcttc caatcatccg cacctcgccc gaccatggca
     9361 ctgcctacga tctggcagga aaaaatcagg catcagaaga ttcattccgt gaggctgtct
     9421 atcttgcctg cgacattgca aaacaacgac aaatcacaag cgaaataact gcaaaccctt
     9481 taaaattttc gcgcatgtct gctgataggt agtatagaaa aagttaagta gcaaacctgt
     9541 gtgtatatac tatcacattg gcacttcata cttattaaat tgcctaacac ctcgctattg
     9601 tttttttaaa tagttttttt actacttttg aactaatgaa tagttttaat tctgatagtc
     9661 attggattga taactatcgg cccgaatgat gttatctgat attgtttagc ggcagtgtaa
     9721 tttcatgttg attgcaagta attaattttt gtaaactgtg taaaacaaaa tataatgaaa
     9781 aatctgattg caattatttt ttgcttactt atttttgaaa ctgtaaatgc acagtggaca
     9841 cagacaaatg gacctgaagg tggggatata tcatcaatag taactgatgg aacaaatctt
     9901 tatgcaggta cttattatgg tggtgtatat aggtcttcaa acaatggtca gatgtggaca
     9961 gctataaata atggcttgac acatattcgt gttggaacac tttggttaaa tggtgcagat
    10021 ttatttgcgg ttacagatgg aggactatat gtgacatcca acaatggaaa ttcgtggtca
    10081 ttaataaata ttgttttacc accattcact tctgttacaa catttgccgc aaatgcaaca
    10141 aaccttttta tt
//
LOCUS       DAOOOL010000093         9926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_630_length_9926_cov_128.328133, whole genome shotgun sequence.
ACCESSION   DAOOOL010000093 DAOOOL010000000
VERSION     DAOOOL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_9926_cov_128.328133"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKU82_08300"
     CDS             <1..651
                     /locus_tag="PKU82_08300"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOZ82912.1"
                     /translation="EKVLNYTTQTYQLSRPNKVGAVMALIRECQPKTIKEWEKWYFEN
                     AKTNEKTPVKITKKSLRELGERLHIKIKEIVIPEWTEAFSQLTLQDCIDYIYNLTINR
                     TYDGFIREKSVIEDNLAKKFPKVKFEESDPELDHAGDIDYLGWVEKRAFGIQIKPVTS
                     KANFGNYSPSERMKASFSSFTKKYGGKVFIVYSIDDKIKNTEVIKQITAEIKKLAK"
     gene            complement(683..1954)
                     /locus_tag="PKU82_08305"
     CDS             complement(683..1954)
                     /locus_tag="PKU82_08305"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ82913.1"
                     /translation="MNIPDLKIPRVVIVGCGFGGLQLAKSLRKARVQIVMFDTNNYHT
                     FQPLLYQVATSGLEPDSIAYPIRKIFKKQKNFLFRWGKVENIIPEKNIIQTTVGELAY
                     DYLVIATGTTTNYFGNADIAQHAMPMKSVIEAINLRSLILQNFEQALLTQSLKEQEAL
                     MTFTVVGGGPTGVELAGALCELKKHVLPNDYPELDFRKMRVLLIEGTNSVLQAMSKEN
                     QHRALKYLDELGCEVWLETQVSSYNGEEVVFKNSKVLPSKNLLWAAGIKGVTISGLNK
                     DAINNRGRYVVDEYCKVKGCENIYAIGDVASMSTADYVNGHPQVAPVAIQMAQLLAEN
                     IELELRQKSLKKFKYFDKGTMATVGRNRAVTESFNIRFGGFAGWLVWMALHLMLLVGF
                     RNKVVVFINWLWNYINYDRNIRLIIPPFKRK"
     gene            complement(2032..2496)
                     /locus_tag="PKU82_08310"
     CDS             complement(2032..2496)
                     /locus_tag="PKU82_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOZ82914.1"
                     /translation="MSEPIKRNEALKPISRDHHHALLLGWKIRQGIKNKIPAERIKKY
                     VDWFWSSYLENHFELEEKNLYPVLGAEHASIKKAMQHHEKLRNLFQSKEQTIQQLDEL
                     QNELDRHIRFEERELFNEIQNAASAKQLQDMAAVHNEAFHDNYSDEFWVIKK"
     gene            complement(2493..3218)
                     /gene="ric"
                     /locus_tag="PKU82_08315"
     CDS             complement(2493..3218)
                     /gene="ric"
                     /locus_tag="PKU82_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOZ82915.1"
                     /translation="MQITKDTIVGDLVAKDYRTATVFKQNGIDFCCNGNRTLEEVCKQ
                     KEMSVTNIIGELTQASQQASAAQTDFNSWPLDLLADYIEKKHHRYVEEKIQEIKPYLD
                     KICKVHGDHHPELYEINQLFNDSAGELAMHMKKEELILFPAVRRMVKSQMQKTTPEKP
                     QFGPINNPIQMMMHEHNNEGERFRQIEALSNKYNPPQDACNTYRVTFAMLKEFEEDLH
                     LHIHLENNILFPKSLELEKAISS"
     gene            complement(3230..4000)
                     /locus_tag="PKU82_08320"
     CDS             complement(3230..4000)
                     /locus_tag="PKU82_08320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ82916.1"
                     /translation="MDTKDIQKAHGHWILAKMGKKVLRPGGKELTKKLIDALHFNDSD
                     NVVEFAPGMGFTASLTLAHKLHSYVGVDADEDIVRMLSKRLQNSQASFILGNASQTNL
                     ESDSKDKVYGEAMLTMHADHRKSEIIREAHRILKKGGWYAIHELGLTDVDDTLKSKIQ
                     RDLALSIKVNARPLTENEWKHLLEQEGFAVKKVMTNGMHLLEMNRIIDDEGLMGATKI
                     GWNILTHPKARKRILEMRKVFKQYQTHMNAIAIVAEKL"
     gene            complement(4170..5102)
                     /locus_tag="PKU82_08325"
     CDS             complement(4170..5102)
                     /locus_tag="PKU82_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HOZ82917.1"
                     /translation="MNNEKLISDIDNKYQQTGENSETYLKGLLHAKPITYWDYVEVDT
                     LLSLQRPRTNFKDEEIFVMYHQVTELVLKMMVHEIKQMVFDPFAEHIWIDKINRLNRY
                     TSMLITSFDVMKYGMNYDDYNTFRSTLAPASGFQSAQFRFIEIYLTRLENLMNEEGRK
                     RLGNNPSIEDYFEHIYWKDAGMDRKTGKKSMTLKLFEEKYLESFIALAKKVKGNTIED
                     KVMQMGNCSDALKTKLKEFDHMYNVAWPIVHLETAQHYLDLKGENKAATGGSEWKKYL
                     HPKYQQRKFFPSLWTKEEIANWGSNEFQKTTNNK"
     gene            complement(5099..5554)
                     /locus_tag="PKU82_08330"
     CDS             complement(5099..5554)
                     /locus_tag="PKU82_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82918.1"
                     /translation="MTVVHLPLALIASTLWIGFVSAISFMEAWLKFKAPGVTIPLGLG
                     IGRLVFNALNKIEWVLATLIICNLILAKEQFINPIKSGHGVFYLIPVFILLFQTVWLL
                     PQLDARAQMHLNRQTVPPSKMHFVYVGLEIVKVVFLATFILNLFKNITT"
     gene            complement(5551..5937)
                     /locus_tag="PKU82_08335"
     CDS             complement(5551..5937)
                     /locus_tag="PKU82_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HOZ82919.1"
                     /translation="MRDIENINDIQLLVDSFYGKIRDDEKLGHIFNGIIKDRWPEHLE
                     KMYRFWQTILLEDQTYFGSPFVPHAKMPVDKTHFEQWIKLFSETVDENFVGEKAEQAK
                     WQGQRMAEMFHYKIEYIKNSSTTPLQ"
     gene            complement(6023..6256)
                     /locus_tag="PKU82_08340"
     CDS             complement(6023..6256)
                     /locus_tag="PKU82_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HOZ82920.1"
                     /translation="MENKEIWLPSLITKTPQEGRELAIKMARKSIAAIQTDPEVRKKL
                     RDDYANDTKQLIASANVVALEFQTVAAANNYWK"
     gene            complement(6310..6744)
                     /locus_tag="PKU82_08345"
     CDS             complement(6310..6744)
                     /locus_tag="PKU82_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOZ82921.1"
                     /translation="MFSKACEHGIKAIIYIATQSMDGRRVKIGDVVENSGSPEAFTAK
                     VLGSLTKYHIVNSYTGPNGGFFIDVKDMKKIKMSDIVNAIDGDSLYNGCGMGLNECSK
                     TQPCPMHDKFIKVRKEIKEMLTTTSVYDLALGLKSGKTVLMR"
     gene            6930..7649
                     /gene="recO"
                     /locus_tag="PKU82_08350"
     CDS             6930..7649
                     /gene="recO"
                     /locus_tag="PKU82_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOZ82922.1"
                     /translation="MSALHNTDGIMLHLSPYGDSSVIAKIYTEKFGIQTYLVNGVRSS
                     KSKNKSVFFQPLSLLQLVVYHRPDKGLQRISNLAFANRFISIPFNITKSTQSIFMAEL
                     LYRTIREEEANPALFRFLFDNIVQLDKSLSANPDFHLLFMIKLSAFLGFRPTDNYEEH
                     LCFDMKEGCFSTKNSSHLYVIDESTSLLLHQCLNSSIKEPQLNYKQRTDLLNVLLQYY
                     QLHLPDGFRLQSMDILTELFN"
     gene            7749..>9926
                     /locus_tag="PKU82_08355"
     CDS             7749..>9926
                     /locus_tag="PKU82_08355"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOZ82923.1"
                     /translation="MEKKRTYLGNADPVAIEALYNSYLKDPQSVDQTWQTFFDGFELA
                     RKSQSPDGGNVVSAADASHIEKEFMVLRLISDYRKRGHLFTDTNPVRERRKYQPTLDI
                     QHFGLSESDLPTLFQAGQQIGIGKASLTDIITHLRDTYCRSVGVEYKYIRSPEVVKWF
                     EQKMESCKNSLEFTIDDKRKILNKLNEAVAFENFLHVKFAGQKRFSLEGAETTIPALD
                     AVIEFGAKMGIKEFVIGMAHRGRLNVLANILNKGYDEIFTEFEGKAFEDNAFGGDVKY
                     HLGYSGDVETYDGKKVHISLTPNPSHLEAVDALVQGIVRSKIDAQGGTTDDIAPILIH
                     GDAAIAAQGVVYEVIQMSLLKGFSTGGTIHLIINNQVGFTTNYIDARSSTYCTDVAKV
                     TLSPVFHVNGDDVEALIYTVKLAMEYRQKYHRDVFIDILCYRKYGHNEGDEPRFTQPL
                     LYKAIASHPNPREIYIQKLLSQGEIETNLVKEMEKSFKALLEEKLSVAKKVEKTKVPN
                     FKDGAWSKLRFSTPEDFLQSPDTSVKQNVLLSIAGKITDLPADKKFFNKITRLFGERK
                     EMLKGEGKLDWAMGELLAYGTLLNEGFPVRLSGQDCERGTFSHRHAVVRVEDSEEQYT
                     PLNHISDKQARYYIYNSLLSEYGVLGFEYGYAFTSPNSLVLWEAQFGDFGNGAQIIID
                     QYLSSAEDKWNRMNGLVMLLPHGYEGQGAEHSSARMERFLTLSA"
BASE COUNT         3008 a         1797 c         1705 g         3416 t
ORIGIN      
        1 gaaaaggttc ttaattatac aacacagacc tatcaattat caaggccgaa taaagtcggt
       61 gctgtaatgg cactcattcg tgagtgtcag cctaaaacta taaaagaatg ggagaaatgg
      121 tactttgaaa atgcaaagac taacgagaaa acaccggtaa aaattactaa gaagagttta
      181 agagaacttg gtgaaagact gcatataaaa atcaaggaga ttgttatccc ggaatggaca
      241 gaagcgttta gtcaattgac attacaagat tgtatagatt acatttacaa ccttactata
      301 aacagaactt atgatggttt tattcgtgag aagtcggtga ttgaagataa tcttgcaaag
      361 aaatttccaa aagttaaatt tgaagaaagc gacccagaac ttgatcatgc cggtgatatt
      421 gactacttgg gctgggttga aaaaagagct tttggtattc agataaaacc tgttacttca
      481 aaagctaact tcggcaatta ttctccgtca gaaagaatga aagcaagttt tagtagcttt
      541 acaaaaaagt atggtggaaa agtttttatt gtttatagca tagatgacaa gattaaaaat
      601 acagaagtga taaaacagat tacagcggaa ataaagaaat tagcaaagtg agtgttttta
      661 cttaaatgat taaagcagta atttacttcc ttttaaaagg cggaataatc aatctgatat
      721 tcctgtcgta gttaatatag ttccaaagcc aatttataaa aacaactact ttatttctga
      781 acccaacaag caacattaag tgtaaagcca tccacaccaa ccatcctgca aatccaccaa
      841 atcgaatatt gaaagattct gttactgcac gatttcttcc aacagttgcc atagtacctt
      901 tgtcaaaata tttgaatttt ttcagtgact tttgtcttag ttccaactca atgttttcag
      961 ccaacagttg tgccatctga attgcaacag gtgctacttg cgggtgtccg ttaacatagt
     1021 ctgctgtgct catggatgca acatcaccaa ttgcataaat attttcacat ccttttactt
     1081 tacaatattc gtcaacaaca tatcgtccac gattattgat ggcatcttta tttaatcccg
     1141 aaatagtaac acctttaatt cctgcagccc aaagcaaatt tttacttggc agcactttac
     1201 tatttttaaa aactacttct tcaccattat aagacgatac ctgcgtttct aaccaaactt
     1261 cacagcctaa ctcatctaaa tattttaatg cacgatgttg gttttcttta ctcattgcct
     1321 gtagcacaga gtttgtacct tcaataagca acacacgcat ttttctgaaa tcaagttcgg
     1381 gataatcgtt cggcagaaca tgttttttca gttcacataa tgctcctgca agctcaacac
     1441 ctgtggggcc accaccaaca acagtaaatg tcataagcgc ctcttgttct tttaaacttt
     1501 gtgtgagtaa tgcctgttca aaattttgga gtattaaact gcggaggttg atagcttcaa
     1561 tgacagattt cattggcatg gcatgttgcg caatgtcagc attaccaaaa taatttgttg
     1621 tagtaccggt agcaattacc agataatcat aagctaattc tccaactgtg gtttgaataa
     1681 tatttttttc gggaataata ttttcaactt ttccccagcg gaaaagaaaa ttcttttgtt
     1741 tcttaaaaat tttccggatg ggataagcaa tagaatcagg ctccaagccc gaagtggcaa
     1801 cctgataaag taaaggttga aaagtgtgat agttgtttgt atcaaacatc actatttgca
     1861 cacgggcttt gcgcaacgat tttgctaatt gtaatccccc aaagccacag cctacaataa
     1921 ccacacgagg aattttcaga tcagggatat tcatagaaaa gttattttaa aaacagatgt
     1981 tagttgtaca tcatgctatg atttagtgcc attggtctga ataccgggaa actatttttt
     2041 aattacccaa aactcatcac tgtaattgtc atgaaatgct tcattgtgca ctgcagccat
     2101 gtcctgcaat tgttttgctg atgctgcatt ttgaatttca ttaaacaact ctctttcttc
     2161 aaaacgaatg tgcctgtcaa gttcattttg taattcatcc aattgttgaa tagtctgctc
     2221 cttactttga aaaagattcc taagcttttc atgatgctgc atagcttttt ttatgcttgc
     2281 atgttcagcc cctaaaacag gatacaggtt tttttcttct aactcaaaat gattttcgag
     2341 atagcttgac caaaaccaat caacatattt tttaattctt tcggcaggaa ttttgttttt
     2401 tattccctgt ctgattttcc agcccagtaa gagggcatga tgatgatcgc gacttattgg
     2461 tttaagtgct tcatttcttt taatcggctc actcatgatg aaattgcttt ttcaagttct
     2521 aatgattttg ggaaaagaat attgttttct aaatgtatat gtagatgtaa atcttcttca
     2581 aattctttga gcatggcaaa cgttactctg taggtgttgc atgcatcttg tggtggattg
     2641 tacttattgc ttaacgcttc aatttgtctg aaacgctcac cttcattatt gtgctcatgc
     2701 atcatcattt gaatcggatt atttattggt ccgaactgtg gtttttccgg tgttgttttt
     2761 tgcatttgcg attttaccat ccttctcact gcaggaaata aaatcaactc ttcctttttc
     2821 atgtgcattg ctaactcgcc tgcagaatca ttaaaaagct gattgatttc atacaactca
     2881 ggatgatgat caccatgaac tttacagatt ttatctaagt aaggctttat ctcctgaatt
     2941 ttctcttcaa cataacggtg atgctttttc tcaatatagt cagcaagtaa atctaatggc
     3001 catgaattaa aatctgtttg tgctgcagat gcttgttgcg aagcttgagt caactcacca
     3061 attatgtttg taactgacat ctctttttgt ttacacacct cttcaagtgt acggttacca
     3121 ttacaacaaa aatctatacc gttttgttta aaaactgttg cagttctata gtctttagca
     3181 actaagtcgc caacaattgt gtcttttgtg atttgcataa tttttatttt taaagttttt
     3241 ctgcaactat tgcaattgca ttcatgtgtg tttgatattg tttgaagact tttcgcattt
     3301 caagaattct ttttctggct tttggatggg tcaggatatt ccatccgatt tttgttgctc
     3361 ccatcaaacc ttcatcatct ataatccgat tcatttcaag aagatgcatg ccgttagtca
     3421 ttactttctt tactgcaaaa ccttcttgct ccaaaagatg tttccactca ttttcagtaa
     3481 gtgggcgtgc attaaccttt attgataaag ctaaatctct ctgaatttta ctttttaagg
     3541 tgtcatctac atcagttagt cctaactcat gaatggcata ccaacctcct tttttgagaa
     3601 tacgatgggc ttctctgata atttcagatt ttctatgatc ggcatgcatt gttagcatgg
     3661 cttcgccata gactttatcc tttgagtcgc tttcgagatt tgtttgtgat gcatttccga
     3721 gaataaaaga tgcttgagaa ttttgtagtc ttttacttaa cattctcact atatcttcgt
     3781 cagcatcaac acctacgtat gaatgtagct tgtgcgcaag tgtcagcgat gctgtaaagc
     3841 ccattccggg tgcaaactca actacattat ccgaatcatt aaaatgtaat gcatcaatta
     3901 atttctttgt aagctctttg cctccggggc gtagcacttt tttgcccatt ttggccaata
     3961 tccaatggcc atgtgctttc tgtatgtctt tagtatccat gattatttta ttatgttgat
     4021 atttcttttt aatattctat aaataataat cacaagtttt gaatctattt gacagtgcag
     4081 gtgttcagtg agaggttatc tgaaagaagt aaggtttttt cagatattaa agcaaacaaa
     4141 ttgctttcag aatcaaaacg gtgatattat tatttgttat ttgttgtttt ttgaaattcg
     4201 ttgcttcccc agttggctat ttcttctttc gtccataatg atgggaagaa ttttctttgc
     4261 tgatattttg gatgtaaata ttttttccat tcacttccac ctgttgcagc tttattttct
     4321 ccttttaaat caagataatg ttgtgctgtt tcaaggtgaa ctataggcca tgcaacatta
     4381 tacatgtgat caaactcctt taacttcgtt tttagtgcat cagagcaatt tcccatttgc
     4441 atcactttat cttctattgt attgcccttt actttttttg caagtgcaat aaaactttcc
     4501 aaatattttt cttcaaacag ttttaatgtc attgattttt ttcctgtttt acgatccatg
     4561 cctgcatctt tccagtagat atgttcaaaa taatcttcta ttgaaggatt gttccctaat
     4621 ctttttctac cttcttcatt cattaaattt tccaatcgtg tcagataaat ttcaataaaa
     4681 cggaattgtg ccgattgaaa tccactagca ggagcaagtg tacttctgaa tgtgttatag
     4741 tcatcatagt tcattccata tttcataaca tcaaaagagg taatgagcat agatgtgtat
     4801 cggttgaggc gattaatttt atcaatccaa atatgttctg caaatggatc aaacaccatt
     4861 tgtttaattt catgcaccat catttttaat accagctcag taacctgatg atacatcaca
     4921 aaaatttctt cgtctttaaa atttgttctt ggtctttgta gtgataaaag ggtatcaact
     4981 tccacatagt cccaataagt aatgggtttt gcatgtaaaa gtcctttcag atatgtttct
     5041 gaattttcac ccgtttgctg atatttatta tcaatatcgc tgattagttt ttcattgttc
     5101 atgttgttat gtttttaaag agatttaaaa taaatgttgc taaaaatacc actttcacaa
     5161 tttcaagacc tacataaaca aaatgcattt ttgaaggggg caccgtttgt ctgtttaaat
     5221 gcatctgtgc gcgtgcatca agttgtggta ataaccaaac tgtctgaaat agtaaaataa
     5281 atacagggat aagataaaat acaccatgtc cacttttgat gggattgata aattgttctt
     5341 ttgctaaaat aagattacag atgattaatg ttgcaagtac ccattcaatt ttgttgagtg
     5401 cattaaatac caatctgcca attcctagac ctaaaggtat tgtaactccc ggtgctttaa
     5461 atttaagcca ggcttccata aaactgattg cactgacaaa accaatccat aatgtggatg
     5521 caattaaagc taatggtaag tgtacaactg tcattgtagt ggagtagtgg aactattttt
     5581 aatatattca atcttgtagt gaaacatttc agccatacgt tggccttgcc attttgcttg
     5641 ctcagctttt tcgccaacaa agttttcatc aacagtttcg gagaagagtt ttatccattg
     5701 ctcgaaatgc gttttgtcaa ccggcatttt agcatgtgga acaaaagggc taccaaaata
     5761 tgtttgatct tctaacaata ttgtttgcca gaaacgatac atcttttcaa gatgttcggg
     5821 ccacctgtct ttaataatac cattaaagat atgacccaat ttttcgtcat cacggatttt
     5881 accatagaag ctatccacca ataattgtat gtcgtttatg ttttcaatat ctctcatcac
     5941 cacttcagaa gtttagtttg ttttaatttt aattttaatg caaactctgt aattgttttt
     6001 ctcatcagta aactataaaa aattacttcc aataattatt tgctgccgcc acagtttgaa
     6061 attctaatgc tactacattg gcagaagcta tcagttgttt tgtgtcgttg gcataatcat
     6121 cacgcaattt ttttcttact tctgggtctg tttgtattgc tgcaattgat tttcttgcca
     6181 ttttaattgc aagctctctt ccttcctgag gtgtttttgt gattaacgag ggtaaccata
     6241 tctctttatt ttccattata tttttatttt gattgtttgt gtactgtaaa attattcgtt
     6301 tgaaaatatt tagcgcatta atactgtttt tccagacttc agtcctaatg ctaaatcata
     6361 aacagaagtt gtagttaaca tttcttttat ttcttttctt actttaataa atttatcatg
     6421 catagggcag ggttgcgttt tgctacactc attcaacccc ataccacagc cattgtataa
     6481 agaatcgcca tcaatggcat taacaatgtc gctcattttt atttttttca tatccttaac
     6541 atcaataaaa aaacctccgt taggtcctgt atatgaatta acgatatgat atttagttaa
     6601 tgagcccaga acttttgcag taaatgcttc cggtgagcct gagttttcta caacatcacc
     6661 aatttttaca cgccttccat ccatggattg tgttgctata tatattatgg cttttatgcc
     6721 atgctcacat gctttagaaa acattgttgc gtcttttcgg ggtacaaaag tagtgagttt
     6781 tttaatttac gacaaaaata tcgtaaataa tttatatata ctatttttca ttctattcag
     6841 gaagtgataa aactgtttta tctttatgct gaatcgtcat catcttgtca taaatttgcc
     6901 acaaaatatt caaatcacta aaaagcggaa tgtctgcctt gcataacaca gatggaatta
     6961 tgttgcacct gtcgccctat ggcgattcat ctgttattgc taaaatttat acggaaaagt
     7021 ttggcataca aacctattta gtcaatggtg tacgttcttc aaaatcaaaa aacaagagtg
     7081 tgttttttca accgttgagt ttgctgcaac ttgttgtata tcatcgtccg gataaaggtt
     7141 tgcaacgcat aagtaacctc gcttttgcca acagatttat ttcaattcca tttaacatta
     7201 caaaaagcac gcagtcaatt tttatggcag agttgcttta tcgtaccatt cgtgaagagg
     7261 aagctaatcc tgctctcttc agatttttgt ttgataacat tgttcaactt gataaatctt
     7321 tatcggctaa tcctgatttc catcttttat ttatgattaa actatctgct tttcttggat
     7381 tcaggccaac agataattat gaggaacatc tgtgttttga tatgaaggaa ggctgtttca
     7441 gcacaaaaaa ttcttcacat ctttatgtta ttgatgaatc aacttcgtta ttattacatc
     7501 aatgtcttaa ttcttcaatc aaagaaccgc agttaaatta taaacagcga acagatttac
     7561 taaatgttct cttacaatat tatcaattac atcttcctga tgggttccgt ttacaaagca
     7621 tggatatact aaccgaactg tttaattgat gagtttttaa acgcagattg gttattcaac
     7681 taactaaaat cagtttttac accggcaaat tgcctacctt tgtgcgctga attcagttaa
     7741 attttaaagt ggaaaagaaa cgtacttacc tcggtaatgc tgacccagta gcaatagaag
     7801 cgctttataa ctcatatctt aaagacccac agtcggtaga tcaaacctgg caaacttttt
     7861 ttgatggatt tgaactggca agaaaaagtc agtctcctga cggtggaaat gttgtttctg
     7921 cagccgatgc atcacatata gaaaaagagt ttatggtatt gcgattaatc agcgattatc
     7981 gcaaacgtgg acatcttttt acggatacca atcctgttcg cgagcgcaga aaatatcaac
     8041 ctacgttaga tattcaacat tttggattat cagagagtga tcttcctaca ctttttcagg
     8101 caggacagca aattggtatc ggtaaagcat cgctgacaga tattattacg cacctacgcg
     8161 acacgtattg ccgaagtgtt ggtgtggaat ataaatatat cagaagcccc gaagtagtta
     8221 agtggtttga acagaaaatg gaaagttgta aaaacagtct tgagtttacc attgacgata
     8281 aaagaaaaat tctgaataag ctgaacgaag ctgttgcctt cgaaaacttt ctgcatgtta
     8341 agtttgcagg gcaaaaacgt ttttctcttg aaggtgccga aacaacaatt cctgctcttg
     8401 atgcagtaat tgaatttggt gctaaaatgg gcatcaaaga atttgtaatt ggcatggcac
     8461 atagaggcag actaaatgta cttgctaata ttctgaataa aggctacgat gaaattttta
     8521 ctgagtttga aggcaaagct tttgaagata atgcctttgg aggtgatgta aaatatcatc
     8581 ttggttactc aggagatgtt gaaacctacg atggcaagaa agtacatatt tctttaacac
     8641 caaacccttc tcacctcgaa gcagtagatg cattggtaca aggaattgtg cgttctaaaa
     8701 ttgatgcaca aggcggaaca acagatgata ttgcacccat actcattcat ggtgatgctg
     8761 ccattgctgc acaaggtgta gtttatgaag tcattcagat gtcgttactc aaaggctttt
     8821 caaccggagg aactattcat cttatcataa acaatcaggt aggttttaca accaattata
     8881 ttgatgcacg gtcaagcacc tattgtaccg atgttgcaaa agttacttta tcaccggtgt
     8941 ttcatgtcaa tggcgatgat gttgaagcac tcatctacac agtgaaatta gccatggaat
     9001 atcgtcagaa atatcatcgc gatgttttta ttgatattct ttgctacaga aaatatggac
     9061 ataacgaagg tgatgaacca cgtttcacgc aaccactttt atataaagct attgcatcac
     9121 atcccaatcc aagagaaata tatattcaga aattactttc acaaggcgag atagaaacaa
     9181 acttagtgaa agaaatggaa aaatctttca aggcattact tgaagaaaaa ttaagcgttg
     9241 ccaaaaaagt tgagaaaaca aaagttccta actttaaaga tggtgcatgg agtaaactcc
     9301 gtttttcaac tcctgaagat tttctgcagt cacctgatac ttctgttaaa caaaatgtgt
     9361 tgctgtcaat tgcaggaaaa attaccgatc ttccggcaga caaaaagttt tttaataaga
     9421 ttacacgact tttcggtgaa agaaaagaaa tgctaaaagg tgaaggtaag ttagattggg
     9481 caatgggcga acttttggct tatggcacat tactcaatga gggttttcct gtgcgcctca
     9541 gcggacaaga ttgtgagcgc ggaacattct cacatcgtca tgcagtagtt cgtgttgaag
     9601 attcagaaga gcaatataca ccactcaatc acatttcaga caagcaagca cgatattaca
     9661 tttacaattc tctgttatca gagtatggag ttttaggatt tgaatatggc tatgcattta
     9721 cttcaccaaa cagtttggtt ttatgggaag ctcagtttgg cgatttcgga aatggagcac
     9781 agataattat tgatcagtat ctgagcagtg cagaagataa atggaatcgc atgaacggat
     9841 tggtaatgtt gctacctcat ggttatgaag ggcaaggtgc cgaacacagc agcgcacgta
     9901 tggaacgctt tcttacacta agtgca
//
LOCUS       DAOOOL010000094         9859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_633_length_9859_cov_46.649225, whole genome shotgun sequence.
ACCESSION   DAOOOL010000094 DAOOOL010000000
VERSION     DAOOOL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_9859_cov_46.649225"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..1423)
                     /locus_tag="PKU82_08360"
     CDS             complement(401..1423)
                     /locus_tag="PKU82_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOZ82924.1"
                     /translation="MNRNIKDISNLLSKLTARRLFNFSKILCSYYLSRITGKANVSGM
                     PVSISMEPTTSCNLRCPECPSGLRAFSRPTGMLQPDFFEKVIDELSPDLLYLILYFQG
                     EPYLNKNLFEMIRYASQKKIYTATSTNAHYLDDDNARKTIESGLDRLIISIDGTTQET
                     YQQYRIGGSLEKVIKGAENMVKWKRKLKSKTPHLIFQFLVVKPNEHQIKEVEQLAKTI
                     GIDEVVYKTAQLYDYEHGNELMPNDLSHSRYKKNKDGTYSIRNPLLNHCWRMWHSCVI
                     TWDGNVVPCCFDKDASHRFGQVATDGFKKVWQSEPYNRFRKLVLKSRSNIDICTNCTE
                     GTKVWT"
     gene            complement(1551..2183)
                     /locus_tag="PKU82_08365"
     CDS             complement(1551..2183)
                     /locus_tag="PKU82_08365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOZ82925.1"
                     /translation="MLHKPAGLMVEEDNFGNPSLQNWFLNHLQTKFPTNKNYYIGFPH
                     RIDRVTEGLIIIAKTKSALQNLNEQFNNKSITKVYHALTEHALPSAEGTLTDYHQKNT
                     KLKKAILSAKKKEGFVRAELRYKEICNTKSNAVLYKIELITGRYHQIRAQFSSRNAAV
                     VGDSFYGAVTNYQQGAIALIASEILFSHPKTNQAMHFTIPLPQNSKWQCR"
     gene            complement(2235..6650)
                     /locus_tag="PKU82_08370"
     CDS             complement(2235..6650)
                     /locus_tag="PKU82_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HOZ82926.1"
                     /translation="MKGIFKTMMTALFIIQATYVLNAQDSKIFWREVSTIPVSGERLT
                     VPSSYRALNLDLNLLQQYLNTAKAEPSQTANLKNGLMLEIPMPDGSFERFSIFQYNVM
                     HPDLAAKFPQIKTYTGQGIDDVTATIKLDVTQFGFHAMIRSSKGDVYIDPYNQNTVNY
                     YMSYERKNLIRQNSFECSLADKTAMEIQNANGNTVQRTNGTQLRTYRLALSCTGEYAA
                     FYGGTVSGAMAGMATTMNRVNGVYESELSIRMVMVANNNLIVYTNSSTDPFTNNNGST
                     MLGQNQTTCDNIIGSSNYDIGHVFSTGGGGVAYLGCVCSSSNKAKGVTGSGSPSGDGF
                     DIDYVAHEMGHQFGGDHTFNSTTGSCSGNRSSTAAYEPGSGITIQAYAGICGSDNLAP
                     HSIAYFHGYSLDQMITFSNTGGGNSCPVTTSTGNTAPVVTNMGYNCTIPISTPFVLTG
                     AATDANGDALTYSWEERDLGPAGAWNVQSTTAPMFRPFPPTTSPSRTFPQMSDVVNNT
                     TTVGELLSNQARTLKFRLTARDSRTGGGGIMHPDSNVIVTVVNAGGAFAVTAPNTAVT
                     WAGNSTQTVTWNVSGTTGSGINTANVKISLSTDGGYTYPTVLLASTPNDGSQTITVPN
                     ISTTTARMKVEAVGNIFFDISNTNFTITTSTGLTTITTSPVSPLSFCKGASLNVPYTV
                     DAAANAGNVFTAQLSNASGSFASPVNIGTLTSTSAGTITCQIPLGTATGAAFRIRVIS
                     SNPSVIGSDNNSNITVNGSPTPSISGTTSFCSGSSTTLNAGSGYSTYLWSTGATTSSI
                     NVSTAGTFTVTVTNASGCTGSTSATTTVNPLPVASITPSGNVTTCNASQLLNAATGVG
                     YNFQWRLNSNNISGATADNYTATVSGIYDVVVTLTGCSATSSQTILTLGTGLPQITAN
                     GSTNICAGANVMLSAPAGASSYQWYRNNVALGTSVNQNYNASSAGQYYCKVTGTCSGN
                     SNVIVISVINNPTPTISSSTALSFCAPGYVALTANTFTGVNYQWQFNSVDIPGATAQT
                     YNATQAGGYRVIQTANGCSKSKTVSVQKANSVIAQINTNDQTTLCSPVGTVNMELVNP
                     IPGYSYQWQKNGVDISGSISTTYAATSSGSYTCKVSASCGTTISNAIPVSIGSFSASV
                     LPAGTVTICTGASVVLSASTGTGFTYQWKNNGVDINGETSPTLTVNTAGSYSVYMTSP
                     CGNATSNVVTVNSATVSAVISPSGIATICAGQIFQMSVTSNPVYTYQWYRNNVAMSGA
                     VNSTYNVTSAGTYYAIVSLNGICPVTTSNLVLTVINNPTPAISASGPTTICTGQDVTL
                     NTNTWPGVVYQWTKNSIDITGATSASYTANTAGGYRVRQSGNGCSKLSPSIQVYVNPC
                     RIAGERNLFETESTLTVFPNPVLQDATITISSDLTVDNGRLLIYDVLGNCVQRAEQLQ
                     SNSFDFNKENLKPGIYFVDFTNGDGFKLTSKFIVQ"
     gene            complement(6878..7462)
                     /locus_tag="PKU82_08375"
     CDS             complement(6878..7462)
                     /locus_tag="PKU82_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82927.1"
                     /translation="MRQVFTVLFLAFTGILHGQNKIEWDGKYQLQLSDFQSPASQIGE
                     IDSYSLLTGSGMEFSFYMNNAEFMFTKNFNSKVNCSFNRNAASLVAPDSLIALDLLSF
                     ARYDFDLSELYARKFRKSLFEEKGAFSDISFFQPIYDKIQQELSERRTIAGKATEIGR
                     NTEKLIELHREVNKEIEQLDQFCKTCKPSKKKSK"
     gene            complement(7528..7941)
                     /locus_tag="PKU82_08380"
     CDS             complement(7528..7941)
                     /locus_tag="PKU82_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82928.1"
                     /translation="MYERAKYFRDKFIRNSIAAFAICGLLIYTVITQHKSVMPGLIIF
                     PAVAFAWLYLGLWINNKLKSKHATDISEVTDEKISFKQVQFIFSIFFVVVVLLYFKHL
                     KIVNYLAVPIILIYAWWLFSQMKMLSNYFSTKPHN"
     gene            complement(8094..8900)
                     /locus_tag="PKU82_08385"
     CDS             complement(8094..8900)
                     /locus_tag="PKU82_08385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ82929.1"
                     /translation="MQYDPIKRSLGEVFNKSPFLRKTFYRLLDLLLLRAWHIHNELKQ
                     WEKTVDNNAHILDAGSGFGQYTYWLASRNKNWKIYAVDVKEEQVSDCNNFFQKAGYKN
                     VQFGIEDLTKYQSPSTYNLVVCVDVMEHILEDEQVFRNYSASLKSGGMLVISTPSDQG
                     GSDVHGDDQQSFIEEHVRDGYNIDDIKAKLLRNGFSRAEAKYSYGTPGKISWRLSMKY
                     PISALGYTKAFFIILPFYYLLFYPICFVLNYLDVNIKHRTGTGLIVKAWK"
     gene            complement(8909..9274)
                     /gene="rbfA"
                     /locus_tag="PKU82_08390"
     CDS             complement(8909..9274)
                     /gene="rbfA"
                     /locus_tag="PKU82_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOZ82930.1"
                     /translation="MDSTRQQKISRMLQKEISSLFQLEGSSFYGNAFVTVTNVRVTPD
                     LGIAYVRLSLFKQKNAEEIVNSLNHKMHDIRRKLGNRIRNQIRHIPELKFFNDESLDY
                     AEHIEKLFKEIHKNDPKQD"
     gene            complement(9297..>9859)
                     /locus_tag="PKU82_08395"
     CDS             complement(9297..>9859)
                     /locus_tag="PKU82_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82931.1"
                     /translation="AIVTTCFNNSLKDVTNSWTTYMKSLKSKKVTAGKEETFTDNVLI
                     KDWGNNPIDIYARFEENKADNSVKVMVAFDLGGAYLSSTVDATKYSTAEQMVKNFAIE
                     TTKAPIQSQLKDAEKLLGKMESDRGSLEKDIKSLRSDVEKYKDNIKKAEDDAYKKEND
                     LSKKKSEIDSQRTAIQLLTSKLGEIK"
BASE COUNT         2903 a         1852 c         1901 g         3203 t
ORIGIN      
        1 atagtcaatg gtaatgatat cagggtggag atgaaggttt ttcaacacct ctttgccagt
       61 gctgaaagcc gttacggcat gatcggggtt caatgacaac acatagtgca gaaattctct
      121 gtaccagtcg tcatcttcta caacgaaaat gcgtgtaatg gaggtggttt tcatcttaca
      181 aaagaaataa aaaaaggttg ccattatgat gaagcaacct tttgaagtgt tgtccgacta
      241 ggattcgaac ctagaaaggc agaaccaaaa tctgcagtgt tacctttaca ccatcggaca
      301 aacgagggcg caaaaatagc atatttttga aaagaaaaaa tcactttttt aaaaaatcta
      361 aatgctgaaa attaaccgtt tatgtaagat ttaaatcctt tcatgtccaa accttggttc
      421 cttcggtgca gttggtacaa atgtcaatat tgcttcgtga cttgagtacc agttttctga
      481 aacggttata aggttcactt tgccacactt ttttgaaccc gtctgttgca acctgcccaa
      541 aacggtgact tgcatcttta tcaaaacagc aaggaacaac attgccatcc caggtaatga
      601 cacatgaatg ccacatccgc cagcaatgat tcaataacgg attacggatg ctgtatgtgc
      661 cgtctttatt ttttttatac cttgaatgtg aaaggtcgtt tggcattaac tcattgccat
      721 gctcataatc ataaagctgt gccgttttat aaaccacctc gtcaatgcct atggttttgg
      781 ctaattgctc aacttcttta atctgatgtt cgttgggctt tacaactaaa aactgaaaaa
      841 tgagatgtgg tgttttggat ttaagtttgc gtttccactt caccatattc tctgcacctt
      901 ttatcacttt ttctaacgac ccaccaatac gatattgctg ataagtttcc tgcgtagttc
      961 catcaattga aattatcagt ctgtcaaggc ctgactcaat ggttttacgt gcattgtcat
     1021 catctaaata atgtgcattg gtagaagtgg ctgtataaat ttttttttgt gatgcatagc
     1081 gtatcatctc aaacaaattt ttgttgagat aaggctctcc ctgaaaatat aagatgagat
     1141 atagcaggtc aggtgaaagt tcatctataa ctttttcaaa aaagtcaggt tgcaacatac
     1201 ctgtgggtct gctgaaagca cgaagtccac tcggacattc agggcagcga agattacagg
     1261 aagttgtagg ctccattgaa atactcactg gcataccgct gacatttgcc tttccggtta
     1321 tgcgtgataa gtaatagctg catagaattt tactgaagtt gaacaaacgt cttgcagtaa
     1381 gcttactcag cagatttgat atgtctttaa tattacggtt caatgggatg gcaacaaaaa
     1441 cacaaaaaat taaaatacaa tttacaaact gctaatcaga aagtaaacac ttgtgcaaag
     1501 ctaatgtttt tatatgctta tttaaatcaa gtcgtttatt gtaaattaca ttaacggcat
     1561 tgccattttg agttttgtgg caacggtatt gtaaaatgca tagcttgatt tgttttggga
     1621 tgagaaaaaa gtatttcaga tgcaattaag gcaatggctc cttgctgata gttggttact
     1681 gcaccataaa atgaatcgcc aacaacagca gcatttctgg aagaaaattg tgcccgtatc
     1741 tgatggtaac gaccggtaat taattcaatt ttatagagaa cagcgtttga cttagtatta
     1801 catatttctt tatatcttaa ctctgctctg acaaaacctt ctttcttctt tgctgaaaga
     1861 attgcttttt taagttttgt gtttttttga tgatagtctg ttagtgtacc ttctgcagaa
     1921 ggtaaagcat gttctgttaa ggcatgataa acttttgtga tgcttttgtt attaaactgt
     1981 tcattgagat tctgcaaagc tgattttgtt ttcgcaatta ttatcaaacc ttctgttaca
     2041 cgatcaatgc gatgcggaaa gcctatgtaa taattcttat tggttgggaa tttagtctga
     2101 agatgattca gaaaccaatt ttgcagcgaa gggtttccaa aattatcctc ttcgaccatt
     2161 aaccctgccg gcttatgcag cacggcaagg taatagtctt caaataagat tgatggttgc
     2221 aaaattttac ttgtttactg aacaataaac tttgatgtca gtttaaatcc atcaccattg
     2281 gtgaaatcaa cgaaatatat tcccggtttc agattttctt tgttgaagtc gaaagaattt
     2341 gattgcagtt gttctgctct ttgcacacaa tttccaagta catcataaat taataatctg
     2401 ccattatcca ctgtaaggtc gctactaata gtaattgttg catcttgtaa aacaggattt
     2461 ggaaaaactg ttaatgtaga ttctgtttca aataaattac gctctcctgc aatgcggcat
     2521 ggatttacat aaacctgaat tgatggtgat agttttgagc aaccgttacc ggattggcga
     2581 actctgtatc cgccagcagt atttgcagta taagaagcag aagttgcacc tgttatatca
     2641 atgctgtttt tagtccattg gtaaaccaca ccgggccagg tattggtatt taaagttaca
     2701 tcctgtccgg tacatattgt tgttggtccg gaggcagaaa ttgcaggtgt tggattatta
     2761 attactgtca acactaaatt agatgttgta accggacaaa taccatttaa agaaactatt
     2821 gcataatagg ttccggcaga agttacatta taagttgaat tgactgcgcc actcattgct
     2881 acattatttc tgtaccactg ataagtataa acagggtttg atgtaactga catttgaaaa
     2941 atctgacctg cgcaaatggt tgctattccg gaaggcgaaa tgactgctga aacagtagca
     3001 ctattcaccg ttacaacatt tgaggttgca ttaccgcaag gcgatgtcat ataaactgaa
     3061 taactgcctg cggtgtttac cgtcaatgta ggagatgttt ctccgttaat gtcaacacca
     3121 ttatttttcc attgataagt gaagcctgtg cccgtacttg ctgataaaac aacagaggca
     3181 cctgtacaaa ttgtaactgt tccggcagga agtactgatg cactgaacga acctattgat
     3241 accggtattg cattggatat agtagtacca catgatgctg ataccttgca tgtataacta
     3301 ccgctggaag ttgctgcata agttgtactt attgaaccac tgatgtcaac accattcttt
     3361 tgccattgat aggaataacc cggaatagga tttaccaatt ccatatttac tgttcctaca
     3421 ggcgaacata atgttgtctg atcgttggta ttaatctgag ctattacact atttgctttt
     3481 tgaacactaa ctgtttttga tttagaacaa ccatttgcgg tttgaataac tcgatagcct
     3541 ccagcttgtg ttgcattgta agtctgagcg gttgctccgg gaatatcaac actgttaaac
     3601 tgccattgat aatttacgcc tgtaaatgta tttgctgtca gtgcaacata acccggtgca
     3661 caaaatgata atgctgttga cgagctgatg gttggtgtag gattatttat tacactgatc
     3721 acaattacat ttgaattacc ggaacaagta cctgttactt tacaataata ctgaccggca
     3781 ctacttgcat tataattctg attgacagaa gttcctaatg ctacattatt tctataccac
     3841 tgataggatg atgcacctgc aggggcagat aacattacat tggctccggc acaaatgttt
     3901 gttgaaccgt ttgctgtaat ctgtggtaat cctgtgccaa gtgttagtat agtttgtgat
     3961 gaggttgctg aacatcctgt taaagtaaca acaacatcgt aaatgcccga tacggtggct
     4021 gtgtaattat cagcagtggc acctgaaata ttgttgctat ttaatcgcca ttggaaatta
     4081 tatccaacac ctgttgcagc atttaataat tgtgatgcat tacatgttgt aacatttcct
     4141 gatggtgtga ttgatgcaac cggtaatgga tttacagttg ttgttgcact tgtgcttcct
     4201 gtacatccac ttgcattggt aacagttact gtaaaagtcc ctgctgttga tacattgatg
     4261 gaagatgttg ttgcaccggt tgaccacaaa taggtactgt atcctgaacc tgcattcaat
     4321 gttgttgaac ttcctgaaca aaatgatgtt gttccactta ttgatggtgt tggactgcca
     4381 ttaactgtta tgttactgtt gttgtcactt ccaattactg atggattaga actaatcaca
     4441 cgaattctga atgctgcacc tgttgcagtg cctaaaggta tctgacaagt aatagttccg
     4501 gcagaggtag aagtaagtgt accaatatta accgggctgg caaatgagcc gcttgcatta
     4561 gaaagttgtg ctgtaaaaac atttcctgca ttggctgctg catcaacagt gtaaggaaca
     4621 tttaatgatg ctcctttaca aaaacttaag ggcgaaaccg gtgatgtggt tattgttgtt
     4681 aatcctgttg atgttgtaat agtaaagttt gtattactga tgtcgaagaa gatgttacca
     4741 acggcttcga ccttcattct tgcagtagta gttgaaatat taggtactgt aatagtctgg
     4801 cttccatcat taggagtaga agctaataaa actgtaggat aggtataacc accatcagta
     4861 cttaatgaaa tttttacgtt agcagtattg ataccacttc ctgttgttcc gcttacattc
     4921 caggttactg tttgtgtgct gttgcctgcc caggttacag cagtgtttgg agcagttact
     4981 gcgaaagcgc cacctgcgtt aacaacagta acaataacgt tagagtcggg atgcatgatg
     5041 ccaccgccac cggtgcggct gtcgcgtgct gttagtctga attttaatgt acgagcctga
     5101 ttggataaca actcacctac tgttgtagtg ttatttacca catcagacat ttgtggaaat
     5161 gtgcgtgatg gactagttgt aggaggaaaa ggacgaaaca ttggtgccgt agttgactgc
     5221 acattccatg caccggcagg gcctaaatct ctctcttccc atgaataagt tagtgcatca
     5281 ccgttggcat ctgttgctgc gcccgtaagc acaaaagggg tactgatagg aatagtacaa
     5341 ttataaccca tattggttac aacgggtgct gtgttgcctg ttgatgtagt tacagggcat
     5401 gaatttcctc ctcctgtatt tgaaaaagta atcatctgat caagactgta accatgaaaa
     5461 tatgcaatgc tgtgaggtgc aagattatcg ctaccacaaa ttcctgcata agcctgaatg
     5521 gtgatgccac tgcccggttc ataagctgca gtagaagatc tgttgccact gcaactacct
     5581 gttgtgctgt taaaagtatg gtcgcctcca aactgatgac ccatttcatg tgccacatag
     5641 tcaatatcaa aaccatcacc tgaaggtgat ccgctaccgg ttacaccttt tgctttattg
     5701 gagctgctgc acacacatcc taaataagcc acacctccac caccggtgct gaatacgtga
     5761 ccaatgtcgt agtttgaact gccaattata ttatcgcaag tagtttgatt ctgccccaac
     5821 atggttgaac cattgttgtt agtaaatgga tctgttgacg aattggtata aacaatcaaa
     5881 ttattattgg caaccatgac catgcgaata gaaagttctg attcataaac accgtttaca
     5941 cgattcatcg tagtagccat tcctgccatt gcaccggaga ctgttcctcc ataaaatgct
     6001 gcatattcgc ctgtacatga caatgcaagt ctgtaggttc gtaactgtgt accgttggtg
     6061 cgctgcactg tgttaccatt tgcattttgt atttccattg ctgttttatc agcaagagaa
     6121 cattcaaaag agttttgtct gattagattc ttcctctcat agctcatgta ataatttaca
     6181 gtgttttgat tgtaaggatc aatataaaca tcaccttttg aagaacgaat catggcatga
     6241 aagccaaatt gtgttacatc taactttatg gttgccgtaa catcgtcaat gccctgaccg
     6301 gtgtatgttt ttatctgcgg aaatttggca gccaaatcgg gatgcattac attgtattga
     6361 aaaatgctga aacgctcaaa agagccatca ggcataggta tctcaagcat caatccgttt
     6421 ttaagatttg ctgtttgtga aggctctgcc tttgccgtgt ttaaatactg ttgtaataaa
     6481 tttaaatcga gattaagtgc cctatagctt gaaggcacag taagtcgttc accggaaaca
     6541 gggatagttg aaacctcgcg ccagaaaatt tttgaatctt gtgcattgag gacataggtt
     6601 gcctggatga tgaacaatgc cgtcatcatt gttttgaaaa tacccttcat ttggaaatcg
     6661 ttttttatta tactttgaag caaaattcct taaaaaaact attaataaca aaggccggag
     6721 taataaatgc acaaatagca gtatagtaag cgagtagccg ttgagaattc agaatgtaga
     6781 attcagagtt cagaattgaa agttgagagt tgagagtcaa aatgtaaaag tgaggggaaa
     6841 tgggaggggt gagccaatta gtattaacat catgccgcta tttacttttc tttttggatg
     6901 gtttacaggt tttacagaat tggtccaact gctcaatttc cttattaact tctctatgta
     6961 actctattaa cttctccgta tttctgccta tctcagtagc ttttccggca atggtacgtc
     7021 tttcggatag ttcttgttga atcttatcat aaataggttg gaaaaaacta atgtcagaaa
     7081 aagccccctt ttcttcaaat aaacttttac gaaattttct cgcatacaat tccgacaaat
     7141 caaaatcata tctagcaaag gaaagcaggt cgagagcaat taaactatcg ggggcaacta
     7201 atgatgcagc atttctatta aaggagcaat taacttttga attaaaattt tttgtaaaca
     7261 taaactctgc attattcatg taaaatgaaa attccatgcc agagcctgta agtaaagagt
     7321 aactgtctat ctcgccaatt tgagaagctg gcgattgaaa atcgcttaac tgcaactggt
     7381 attttccatc ccactcaatc ttattttgtc catgtagaat gccagtaaaa gctaaaaaca
     7441 atactgtaaa tacctgcctc atatatctct tgaaatatgc tttcctgtta tgcttaaatt
     7501 aaggcaacta atttatgttt ttaaatttta attgtgtggt ttcgtactaa aataattact
     7561 cagcattttc atttgcgaaa acagccacca tgcatatatt aagattatgg ggacggccaa
     7621 atagttaaca attttcaaat gtttgaagta aagtaaaaca accacaacaa aaaaaattga
     7681 aaaaataaac tgaacttgtt taaatgaaat cttttcatca gttacttctg atatgtctgt
     7741 tgcatgtttg gatttcagtt tattatttat ccataagcct aaatacagcc atgcaaaagc
     7801 tacagccgga aaaattatca gtccgggcat cactgatttg tgttgtgtta taactgtgta
     7861 aattaacaat ccgcatattg caaatgcagc aatgctgtta cgtataaatt tatctctaaa
     7921 atacttggct cgctcataca tgacaaatag tgttttaaaa aattaaactg tttgggaaca
     7981 taagcaaata tgaatgtaaa attgagaatt taaactgaaa aagagatgag atttcaaaat
     8041 gcagagttta gaatacagaa ttaaaagtta gctagcactt tcaaccacaa aacctatttc
     8101 caagctttta cgatgaggcc ggtgcctgtg cgatgcttta tgtttacgtc taaatagttt
     8161 agcacaaaac aaattggata aaacagcagg taatagaatg gaagaataat aaaaaatgcc
     8221 ttggtatatc ccaatgcaga gataggatac ttcatagata agcgccagga gatttttccg
     8281 ggagtgccgt aagaatactt tgcttcggca cgactgaagc catttcgcag cagctttgct
     8341 tttatatcgt caatgttata gccatcgcga acatgctctt cgatgaaaga ttgttggtca
     8401 tcgccatgaa catcactgcc accctgatcg cttggtgttg aaatgactaa catgccaccg
     8461 cttttgagtg atgcagaata atttctgaaa acctgctcat cttcaagaat gtgctccatt
     8521 acatcaacac aaacaacaag attgtatgtt gagggcgact ggtatttggt taaatcttca
     8581 atgccgaatt gtacgttttt atatcctgcc ttctgaaaaa aattattgca atcgctgacc
     8641 tgctcttctt tgacatctac tgcataaatt ttccaattct tgtttcgtga agcaagccag
     8701 taggtgtatt ggccaaagcc tgagccagca tctaaaatat gtgcattgtt gtcaactgtt
     8761 ttttcccatt gctttagctc attatggatg tgccaggcgc gcaggagcaa taaatcgagc
     8821 agacggtaaa atgtttttct taaaaatggt gatttattaa acacctcgcc cagagagcgc
     8881 tttataggat cgtattgcat tcagtgtgtc agtcttgctt tggatcgttt ttatggattt
     8941 ctttaaacag cttttcaata tgctcggcat agtctaagct ctcatcatta aaaaatttca
     9001 gctcaggaat atgtcttatc tgattgcgga ttctgttacc taactttctt ctgatgtcgt
     9061 gcatcttatg gttcaggctg tttactatct cttcagcatt tttctgcttg aataaactta
     9121 aacgcacata ggcaatgcca aggtcaggcg ttactctgac attggtgaca gtaacaaagg
     9181 catttccata aaacgaactg ccttcgagct gaaacaaact actgatttct ttttgcaaca
     9241 ttcttgaaat tttctgctgc cttgtgctgt ccattaactt attcttaaat taaaatttat
     9301 ttgatttcgc ccagcttact tgtgagaagc tgtatggcag tacgctgact gtcaatttcg
     9361 cttttcttct tgctcaggtc attttctttt ttgtaggcat cgtcttcggc tttcttaata
     9421 ttatctttat atttttcaac atcgctgcgc aatgacttaa tgtctttttc caaagagcct
     9481 ctgtcgcttt ccatcttacc caacaatttt tctgcatctt ttaattgtga ctgaataggt
     9541 gctttggttg tttctattgc aaaattttta accatctgct ctgctgtact atatttagtg
     9601 gcatcaacag ttgatgataa ataagcccct cctaaatcaa atgcaaccat cactttaaca
     9661 ctgttgtcgg ctttgttttc ttcaaaacga gcataaatgt caattggatt attgccccag
     9721 tcttttatca gaacattgtc ggtaaaagtt tcctctttac ctgctgttac ttttttactt
     9781 tttaaagatt tcatataggt tgtccagcta ttggtaacat ccttgaggct gttgttaaag
     9841 caagttgtaa caattgcag
//
LOCUS       DAOOOL010000095         9853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_634_length_9853_cov_96.739539, whole genome shotgun sequence.
ACCESSION   DAOOOL010000095 DAOOOL010000000
VERSION     DAOOOL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_9853_cov_96.739539"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1442
                     /locus_tag="PKU82_08400"
     CDS             15..1442
                     /locus_tag="PKU82_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82932.1"
                     /translation="MTKERFFNFIQNPLSVHANDIEELEQIIEQFPYFQTARLIYTKS
                     LHNENSYLYNDELKRTAAFAADRSVLYKLIHSIKKDVTIDEPESLITEDIGSKSVINP
                     VQEVPDLNVFVFEEKTEIEEQAIVFEEKENSESADDELIIFEEKTLTIEEGEPIVFEE
                     KRPEITTQEDIVFTAVDEPINASAEDDEVGSSNKIAEIESEKKERVLTPAEILSQRLR
                     EIESQLEDKKEDETEVVSELKEETIIPETPQIAEPLNIQSEVLPESIQISSSIPEIKE
                     EVAEISEQAEAEKLIPAEPESSEKTIEVVAISPQIQPPVIEEVVAPDAIVQAEIKPLP
                     EADKKEKHSFTDWLHRFHPQEEKKTLSPDNLEEDNYNNKLINSQLTSYIETKNSNNEI
                     VNQFIQNEPRIDSSKTKFFSPGNMAKSSVTDVSDIVSETLAKIYLGQNNFSKAIQTYE
                     KLMLKYPEKSVYFAALIKEIKKSQI"
     gene            1455..1820
                     /gene="secG"
                     /locus_tag="PKU82_08405"
     CDS             1455..1820
                     /gene="secG"
                     /locus_tag="PKU82_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOZ82933.1"
                     /translation="MYTLITVLIILISVLLCLAVLVQNSKGGGLASGFSAPQQIMGVR
                     KTTDFLEKFTWGLGTALIVFCVVANFMTPGSNNSTEVRASSIQEQIDNATLPAAPQAP
                     LPSQQNAQPAQQPAAQPAN"
     gene            2003..2281
                     /gene="groES"
                     /locus_tag="PKU82_08410"
     CDS             2003..2281
                     /gene="groES"
                     /locus_tag="PKU82_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOZ82934.1"
                     /translation="MSKISLKPLADRVIVEVAAAEEKTASGIIIPDTAKEKPQKGKVV
                     AVGGGKKDEPMTVKVGDSVLYGKYAGTEISVEGKEYLIMRESDIFAIV"
     gene            2335..3966
                     /gene="groL"
                     /locus_tag="PKU82_08415"
     CDS             2335..3966
                     /gene="groL"
                     /locus_tag="PKU82_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611460.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOZ82935.1"
                     /translation="MAKEITFNLDARDALKKGVDALANAVKVTLGPKGRNVIIDKKFG
                     SPTITKDGVTVAKEIELTNSIENMGAQMLKEVASKTADIAGDGTTTATVLAQSIVNTG
                     LKNVTAGANPMDLKRGIDKAVEAVVASLKKMSKEVGDDNKKIEQVATISANNDSEIGK
                     LIAEAMAKVKREGVITVEEAKGTETTVEIVEGMQFDRGYISPYFVTNADKMQAVLESP
                     YILLYDKKVSNMKELLPILEKVVQTGKPLLIIAEDVDGEALATLVVNKIRGSLKIAAV
                     KSPGFGDRRKAMMEDIAILTGGTLIAEEQGFKLENADLSYLGTCEKITIDKDNTTIVG
                     GAGKKADITARVNQIKAQIETTTSDYDKEKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVAYIRAITALDKLKGDNDDETTGIAIIKRALEEP
                     LRQIVSNAGIEGSIVVQKVKEGKDDFGYNARSDKYENMHAAGVIDPTKVTRVALENAA
                     SVAGMLLTTECVLADIKEDKPAMPAMPGGMGGMDY"
     gene            complement(4245..5936)
                     /locus_tag="PKU82_08420"
     CDS             complement(4245..5936)
                     /locus_tag="PKU82_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOZ82936.1"
                     /translation="MKNLILTGLTIGLTCMMSYAQKYDEMKTTWEIETTHPFTETGLS
                     EKKEIVHGSNEKMFTMVSAVDGKVKWSKKFKEIDEQIGKVDLQIPMYESNAVFLFDKK
                     AGKDQMVVVDMETGTKLWSTNKYQGIDDPDEVIYIAELNAYAIVTKTNLTMVKARTGE
                     ELWSTVKFSTPVGKYFYDEKENAIVIISMPRNAWQAMFKGFRNQILKINAKNGDILWE
                     QTFVGIVERKVVTRESVAKIFLRDDKLFLQLNGLQVYEYKTGSPIWAAAYDVTFAREL
                     VRPKVYGQVVKYGVYDAIAQPLYDGEYVYILDVKSRKSQYLKKYEINSGKLVWSSPEI
                     KDARVIPGLYKIDDRIIVQIGGAVEVQAISKETYGNGYVQWTQYYRTVKYQNVKPYEV
                     QAFSTSDGTKSWDSERFRKGITNIFPSGNNIVVSSGKALYSLDYKTGKEAYEIPLGDD
                     DIGLAEKIVNPTELGDNISKDNVIVLGEKGVSSHNIATGAKVWAVKTKKGEFSGIFGE
                     NIFFDTEKGDYFSIDANSGKAKFFDSRKNSKSIVSLDGKSIYVFEGKKILKVSTE"
     gene            complement(5978..7489)
                     /locus_tag="PKU82_08425"
     CDS             complement(5978..7489)
                     /locus_tag="PKU82_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82937.1"
                     /translation="MKPILLAILSLAFVTTVYAQPAGTRTITIDDCNYPILGIETNHA
                     SDKIVLTASQGRIFFINVSDVEANTEVNPVWKNLNLSGLNNGGKPQFSENDQYILLQE
                     SSALKTAITSRKVKDIKYYILNAADGKVVSEGNNVNSVQFLSNSTQILIASNDGIMVK
                     DFLSNKVIAQLEVDNCEMAAISHDNKLIALSFDPDKADFKEVESIGKNKSELKTARKN
                     KRLISFYSYPDLKKLYNSKEEIDIVYSIKFSRDDKQTYLLSHNVVAERIGTAGLNGIS
                     MAAADNRRQTNIQVLDASNGNLVKEIYHATAEPDADYKFSTYSNYFGYSDNDGFIGWK
                     RKLVLIDNNDHKNKVGQYRYQGRRGSSNIFPIYFSFNKTKPVAYLSNGTKLIEWNYET
                     LKEYTDYIEGQDNDSLQSKAISQLNADLENPESDLSKNIVKQNIKGIYLFDLTVQKKG
                     QIVTIFAESDDKTDIPHQNALKDLLLKYKFKDIVVSPNQRLKFRYTFSIENNI"
     gene            complement(7926..8261)
                     /locus_tag="PKU82_08430"
     CDS             complement(7926..8261)
                     /locus_tag="PKU82_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82938.1"
                     /translation="MEQLSNQNFQQRNLIEQTVVQLNKDLIPCGFTVAWSGDTFSAYQ
                     DIIMQLEVIIRQLAKSGSSTLNAWLYRVDIPEKTLKKVLNKQDNQQALAQAILERTFI
                     KIMFRNSYR"
     gene            complement(8283..8708)
                     /gene="aroQ"
                     /locus_tag="PKU82_08435"
     CDS             complement(8283..8708)
                     /gene="aroQ"
                     /locus_tag="PKU82_08435"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOZ82939.1"
                     /translation="MAKRIVIINGPNLNLLGKREPEIYGHQTFDDFAITLKKEFPEPN
                     IQIFQSNVEGEIINLLHNEGFKADGIIINAGGYTHTSVAIADAIAAIPSPVVEVHISN
                     IHSREEYRHKSLLAANCKGVITGFGLNSYRLALLALLNS"
     gene            complement(8715..9296)
                     /locus_tag="PKU82_08440"
     CDS             complement(8715..9296)
                     /locus_tag="PKU82_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOZ82940.1"
                     /translation="MEKFYTIQGEGYHTGKPAFFIRLAGCDVGCVWCDVKESWEASTS
                     NLISVDNLLKAVAESKTPNVVITGGEPAAYNLTKLTDALSKKGYKVWLETSGAYPIQG
                     TFDWICVSPKKFKAPLESVLQKANELKVVIFNESDFKWAEENARKASDVAKLFLQPEY
                     SRFDRVIPIMIDYIKRNPQWSISLQTHKYLNIP"
     gene            complement(9378..>9853)
                     /locus_tag="PKU82_08445"
     CDS             complement(9378..>9853)
                     /locus_tag="PKU82_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOZ82941.1"
                     /translation="KPDKETVDLWTKLKKIGGMSPKITYKDIVYIAVRDIEPQEAYLL
                     KKHNVKMISAAEVKRIAVEKIVRTALAHLSHCTHIYVSFDVDSMDSSISKGTGTPVRN
                     GISERDAGKLCVRLVQNEKVCCFEICEVNPTLDKENLMAENTFEILQRVVNQINH"
BASE COUNT         3240 a         1782 c         1718 g         3113 t
ORIGIN      
        1 gttgttaact ggtaatgact aaggaacgtt tttttaattt cattcaaaat ccgttaagcg
       61 ttcatgccaa tgacatcgaa gagttggaac aaatcattga gcagttcccc tattttcaga
      121 ctgccaggtt gatttatacc aaatcactgc acaatgaaaa cagttatttg tacaatgatg
      181 aattaaagcg aactgctgcc tttgctgccg acaggagtgt tctttataaa ctcattcatt
      241 caattaaaaa agacgttaca attgatgagc cggaaagttt gattacagaa gatataggat
      301 caaaatcggt aattaatcct gttcaagaag tacctgattt gaatgttttt gtatttgaag
      361 aaaaaacaga aattgaagag caggcaattg tatttgaaga aaaagaaaat tctgaatcag
      421 cagatgatga actcataata tttgaagaaa agacattaac aattgaggag ggcgagccga
      481 ttgtttttga agaaaagcga ccagaaatta ccacacagga agatatagtt tttacagcag
      541 ttgacgaacc catcaatgcc agtgcagaag atgatgaagt tgggtcttca aataaaattg
      601 cagaaataga gtcagagaaa aaagagcgtg tgctgacacc tgcagaaatt ttatctcaaa
      661 gactacgaga aatcgaatct caattagaag acaaaaaaga agatgaaact gaagtagtct
      721 ctgaattgaa agaagaaaca attattcctg aaacacctca aattgcagaa cctttaaata
      781 ttcaatctga agttttgccg gaaagcattc aaattagttc ttctataccg gaaattaagg
      841 aagaagtagc agagatttct gaacaagcag aagcagaaaa actaataccc gctgaacccg
      901 aaagttcaga aaaaacaatt gaagttgttg caataagtcc tcaaattcaa ccacccgtaa
      961 ttgaagaagt tgttgcacct gatgcaatag ttcaagctga aataaaacca ttgccagagg
     1021 ctgataaaaa agaaaaacat tcgtttacag actggctgca tcgttttcat ccacaggagg
     1081 aaaaaaaaac tttaagccct gataatttag aagaggataa ctataacaac aaactgatta
     1141 acagtcaatt aacgtcatac attgaaacta aaaattcaaa taatgaaata gttaatcagt
     1201 ttatccaaaa cgaacccagg attgacagta gcaaaactaa gtttttcagt ccgggcaata
     1261 tggccaagtc aagtgttact gatgtatctg acatagtttc agaaacgctg gcaaaaatat
     1321 atttagggca aaacaatttc agcaaggcta ttcaaacata cgagaagctg atgttgaaat
     1381 atccggaaaa aagtgtttac tttgcagccc ttattaaaga aataaaaaaa tcacagattt
     1441 aacattcaat ctatatgtac actttaatta ccgtgttaat tattttaatt agtgttttac
     1501 tttgccttgc cgtattggta caaaactcaa aaggtggcgg tttagcttcc ggattttctg
     1561 caccacagca aattatgggc gttcgtaaaa caacagactt tcttgagaaa tttacttggg
     1621 gattaggaac tgctttaatc gttttctgcg ttgtagcaaa ttttatgact ccaggcagta
     1681 ataacagtac tgaagtaaga gcttcttcta tacaagagca aattgacaat gcaacgcttc
     1741 ctgctgcacc acaggcacca ttgccatcgc agcaaaatgc acaacctgca cagcaacctg
     1801 ctgcacagcc tgcaaattaa tcttgtcagt taataacttt taaaggtgcc ataatgctga
     1861 aattatgaca cctttattat ttaagccaaa gccataaatc gcaaaactgt cagtaaaata
     1921 gtcatggcac agaaactgat aaaggcaaat agaaattaat tataaagtat aaaagtcaaa
     1981 cattttaaat cacattatta ctatgagtaa aattagttta aaaccattgg cagaccgcgt
     2041 tattgtagaa gtggctgcag cagaagaaaa aacagcaagt ggaattatta ttcctgacac
     2101 agcaaaagaa aaacctcaaa aaggcaaagt agtagctgtt ggtggtggca aaaaagatga
     2161 gccaatgact gtaaaagttg gtgacagcgt attgtacgga aaatatgcag gaacagaaat
     2221 ttctgttgaa ggcaaagagt accttatcat gcgtgaatca gacatcttcg caattgttta
     2281 aaagctttag aaaacaaaaa gaaataaata aacaacttaa ataaaatacg aaaaatggca
     2341 aaagaaatca catttaacct tgatgcacgc gatgctctta aaaaaggtgt tgatgcattg
     2401 gcaaatgcag taaaagtaac tttaggacct aaaggccgca acgttattat tgataaaaaa
     2461 ttcggatcac caacaattac aaaagatggt gttaccgttg caaaagaaat agaactcacg
     2521 aacagtattg agaacatggg tgctcagatg ctgaaagaag tagcgtcaaa aactgctgac
     2581 attgcaggtg atggtacaac aacagctaca gtacttgctc agtcaattgt aaacacaggt
     2641 ttaaagaacg ttactgccgg tgccaatcca atggatttaa aacgcggtat tgacaaagct
     2701 gttgaagctg tagttgcatc attaaagaaa atgagcaaag aagttggtga cgacaataag
     2761 aaaattgagc aggtggctac aatttctgca aacaacgata gcgaaatagg aaagcttatt
     2821 gctgaagcaa tggctaaagt aaaaagagaa ggtgttatta ctgttgaaga agcaaaaggt
     2881 actgaaacta cagttgaaat tgtagaaggt atgcagtttg acagaggata tatctctcca
     2941 tactttgtta ccaatgcaga taaaatgcag gctgttttag aaagcccata cattttattg
     3001 tacgacaaaa aagtatctaa catgaaagaa ttacttccaa tattagagaa agtagttcag
     3061 acaggaaagc ctttgttgat tattgctgaa gatgtagatg gcgaagcatt agctacattg
     3121 gtagtaaaca aaatccgtgg ctcactgaaa attgcagcag taaaatctcc tggctttggc
     3181 gatcgcagaa aagcaatgat ggaagatatt gcaatcctta caggcggcac attaatcgct
     3241 gaagagcaag gattcaaatt agaaaatgct gacctctcct acttaggcac atgcgaaaaa
     3301 atcactattg ataaagacaa cacaacaatt gttggtggtg caggaaaaaa agctgacatc
     3361 actgctcgtg ttaatcagat taaagcacag atagaaacta caacatctga ctacgacaaa
     3421 gaaaaacttc aggagcgttt ggcaaaatta gcaggtggtg ttgctgtact ttatgttggt
     3481 gcagctacag aagtagaaat gaaagaaaag aaagaccgtg ttgacgatgc attacatgct
     3541 acacgtgcag cagttgaaga aggtattgtt gcaggtggtg gtgttgcata cattcgtgca
     3601 ataacagctt tagacaaact taaaggtgat aacgatgatg aaacaacagg aattgcaatc
     3661 atcaaacgtg cacttgaaga gccattacgt cagattgtga gcaatgccgg aattgaagga
     3721 agcattgtag tacaaaaagt aaaagaaggc aaagacgact ttggttacaa tgcccgctca
     3781 gacaagtatg aaaacatgca tgctgccgga gttattgatc ctactaaagt aacacgtgtt
     3841 gctcttgaaa atgcagcttc tgttgctggg atgttactca ctacagagtg tgtacttgcc
     3901 gacattaaag aagataagcc tgcaatgcct gcaatgccgg gtggaatggg cggaatggat
     3961 tattaatcat ctgcaatttc aaagtacgga aaacgtattt cggaacaata aacaaaaccc
     4021 tctgttggat tttaccttca gagggttttt tatttgtcag ttgtaaggtt tagaaagttg
     4081 gaatgtttgg aaaattggct ttactctata atcaaacata taataattct actgtcctca
     4141 ctaagttcct atcttaccta ctccgctatt ttgctataat ccaaaataaa aaagtcccga
     4201 catttttttg ccgggacttt tttataaata atttaaaact ttctttattc agtagatact
     4261 ttcaggattt tctttccttc aaacacataa atgctttttc catctaaaga aacaatagat
     4321 tttgaatttt tgcgtgagtc gaagaatttt gctttaccgg aattggcatc aatagaaaaa
     4381 taatcgcctt tctctgtatc aaagaaaata ttttctccga aaattcctga aaactctcct
     4441 tttttggttt ttactgccca cacttttgca ccagtagcaa tgttatgaga agaaacgcct
     4501 ttctctccca aaacaattac attgtcttta ctaatattat cacctaattc agttggattt
     4561 acaatttttt ctgccaagcc aatatcatca tcacctaaag gaatttcgta ggcttctttg
     4621 cctgttttgt aatctaaaga gtataatgcc ttaccacttg acactacaat gttattgccg
     4681 cttgggaata tatttgtaat tcctttccta aagcgctcag agtcccaaga ttttgtacca
     4741 tctgaagtac taaacgcctg cacttcataa ggctttacat tctgatattt tacagttctg
     4801 taatattgtg tccattgtac atagccgtta ccataagttt cttttgatat agcctgcact
     4861 tcaacagcgc ctccaatttg cacaattatt ctatcgtcaa ttttatacaa gcccggaata
     4921 actcttgcat ctttaatttc aggagaagac caaacaagtt tacctgagtt gatttcgtat
     4981 ttctttaaat actgactttt tctgctcttt acatctaaaa tatatacgta ctctccgtca
     5041 tacaatggtt gtgcaatggc atcataaaca ccatatttta caacctggcc atatactttg
     5101 ggcctcacta attctcttgc aaaagttaca tcgtaagctg ctgcccaaat tggacttcct
     5161 gttttatatt cataaacctg caagccattt aattgtaaaa acaacttatc gtctcttaaa
     5221 aatattttag caacagattc gcgggtaacg acttttcttt ctactattcc aacaaaggtt
     5281 tgctcccata aaatatcacc attcttagcg ttgattttca gaatctgatt tctaaaacct
     5341 ttaaacatgg cctgccatgc atttctgggc atgctaatga tgacaattgc attttctttt
     5401 tcatcataaa agtatttacc aaccggtgta ctaaatttaa ctgttgacca aagttcctct
     5461 ccagttctgg cttttaccat ggttaaattt gttttggtta caatagcata agcatttaat
     5521 tctgcaatgt aaataacctc atccggatcg tcaatgcctt gatatttatt ggtactccaa
     5581 agtttagtac ctgtttccat gtcaaccacc accatttggt ctttacctgc ttttttatca
     5641 aataaaaaca ctgcattcga ttcgtacatt ggaatttgta aatctacttt cccaatttgc
     5701 tcatcaattt ctttaaactt tttactccat tttactttac cgtcaacagc ggaaaccatg
     5761 gtaaacattt tttcattgct accatgtacg atttctttct tttcgctcaa tccggtttct
     5821 gtaaacggat gtgtggtttc tatctcccac gtggttttca tttcatcgta tttctgagcg
     5881 tagctcatca tacaggtcag acctattgtc agaccggtta aaattagatt tttcattttt
     5941 tattgtttgt tttaattaat taaaatttat ctttttgtta tatgttattt tcaatactaa
     6001 aggtgtatct gaatttcagt cgttgatttg gagaaacaac aatgtcttta aatttatatt
     6061 tgagtaacaa atcttttaaa gcattttgat gagggatgtc agttttatca tccgattcag
     6121 caaagatggt aactatctgt ccttttttct gaacagttaa atcaaataag tatatacctt
     6181 ttatgttctg ctttactata ttttttgaca aatcactctc cggattttct aagtcggcat
     6241 taagctgact aattgctttt gattgtaagg agtcgttatc ttgtccttca atataatcag
     6301 tatattcttt taaggtttca taattccatt ctatcagctt tgtgccattg gataagtagg
     6361 ctacaggttt tgttttatta aaggaaaaat aaataggaaa aatatttgag cttcctctcc
     6421 tgccttggta acggtattgc ccaactttgt ttttatgatc gttattatca attaatacga
     6481 gtttacgctt ccatccaata aatccgtcat tgtctgaata tccgaaataa ttagagtaag
     6541 tactaaactt gtaatcggca tcaggttcgg cagttgcatg ataaatttct ttaactaagt
     6601 taccattgct tgcatctaaa acctgaatat tagtttgtct tctattatcg gcagcagcca
     6661 ttgatatgcc atttaaacct gctgtaccaa tgcgttcagc tactacgtta tgcgagagta
     6721 aataagtttg cttatcatct ctgctgaatt taatagaata aacaatgtct atttcttctt
     6781 ttgagttata aagtttcttt aaatcggggt agctgtaaaa gctgattagt cgcttgttct
     6841 ttctggccgt ttttaactca cttttatttt taccaataga ttctacttct ttaaagtcag
     6901 ctttatcggg atcaaaagaa agtgcaatta atttattgtc gtggctgatg gctgccattt
     6961 cgcaattatc tacttctaat tgagctatta ctttgtttga taaaaagtct ttcaccataa
     7021 tgccatcatt tgatgctatt aaaatttgtg tgctgttgct taaaaactgt actgagttta
     7081 cattgtttcc ttcagaaaca acttttccgt cagcggcatt taaaatataa tattttatat
     7141 cttttacctt tcttgaggta attgcagttt ttaaagctga cgattcctgc aataatatat
     7201 attggtcgtt ctcgctaaat tgaggtttac caccattgtt tagcccggaa aggtttaaat
     7261 ttttccaaac agggtttacc tctgtgttgg cttcaacatc gcttacgtta ataaaaaaga
     7321 tacgtccttg cgaggcggtt aaaactattt tatcagaggc atgattagtt tcaatgccta
     7381 atatgggata gttgcaatcg tctattgtta tagttcgggt tccggcaggt tgggcataaa
     7441 cagttgttac aaaagcaaga ctgaggatag ctaatagaat gggcttcata agttaattgt
     7501 aaagttcttt ttcatatttg tattgtatgc agttgacacc actttcaatg atatgttaat
     7561 acgataaaca gaaattaatg ttacaaagtg tttttgaatg ccatctgtcg tgagatactt
     7621 tctaaagaaa aaagaacctc gcaaagactg caaagaatgt aacacgcaaa gactgcaaag
     7681 aatgtaacac gcaaagactg caaagaatgt aacacgcaga gaccactaag aatgtaacac
     7741 gcaaagaccg caaagaatat aacacgcaaa gagcgcaaag aaaaagttga actcatggta
     7801 ttctatgtaa atcttatttt atagttctac taaaaggata gcctttctga actaaaaaat
     7861 attttcaaaa acaattcaac aactttcaaa ctttgcaact ttctaaactt tccaaactca
     7921 aaggcttaac gataactatt cctgaacata attttaataa acgttctttc aagtatggcc
     7981 tgtgctagtg cttgctgatt atcctgttta tttaaaactt tctttaatgt tttctcagga
     8041 atgtcaactc tataaagcca tgcatttaaa gttgaagagc ctgattttgc caactgacgg
     8101 ataattactt ctaattgcat tataatatcc tgataagctg aaaaagtatc gccactccat
     8161 gcaacagtaa atccacaagg aattaaatct ttgtttaatt gcacaactgt ttgttcaatt
     8221 aagttacgtt gctgaaaatt ctgattagaa agttgttcca tttccatcct gctcttctta
     8281 agtcaggagt ttagcaatgc aagcagtgca agacggtagg aatttaatcc aaatcctgtg
     8341 attacacctt tacaattggc agcaagcaga cttttatgtc tgtactcttc gcgtgaatga
     8401 atattggaaa tatgcacttc aaccaccggt gacggtattg ccgcaatcgc atctgctatg
     8461 gcaacacttg tatgtgtata acctcctgca ttgatgatga taccatcggc tttaaatcct
     8521 tcgttatgca aaaggttaat aatttcacct tcaacattac tctgaaatat ttgaatattg
     8581 ggttcaggaa attctttttt taatgttatt gcaaaatcat caaatgtctg atgaccataa
     8641 atttcgggct cacgtttgcc aagaagattc aggttagggc cgttgatgat tacaattctt
     8701 tttgccataa aaaattatgg gatgtttagg tacttatgcg tttgtagtga aattgaccac
     8761 tgaggatttc tctttatgta atcaatcatt atcggaataa ctctatcaaa cctactgtat
     8821 tcaggttgta gaaaaagttt tgcaacatca gaggctttac gtgcattttc ttcagcccat
     8881 ttaaaatccg actcattaaa aataacaacc ttcagttcat ttgctttttg taaaacagat
     8941 tccaacggtg ctttaaattt tttaggcgaa acacaaatcc agtcaaaggt accctgaatt
     9001 ggataagcac ctgatgtttc aagccacact ttatatcctt tcttggatag tgcatcggta
     9061 agtttggtta gattatatgc tgccggctcg cctcctgtaa taacaacatt aggcgtttta
     9121 ctttctgcca cagctttcaa cagattatca acagaaatca gatttgatgt tgaagcctcc
     9181 catgattctt taacatcaca ccaaacacaa cccacatcac agcctgcaag tctgataaaa
     9241 aaagcaggtt taccggtatg atagccttcg ccctgaatgg tgtaaaattt ttccattaaa
     9301 ggataatatc cttccggcag attggcgaga atattctttt tgtaattttc agtcacggca
     9361 ggttgcattg aaaatcatca atgattgatc tgatttacta cacgctgtaa aatttcgaaa
     9421 gtattttcag ccatcagatt ttctttatca agtgttggat tgacttcgca aatttcgaaa
     9481 caacacactt tttcattttg caccaacctt acacacagtt ttcctgcatc acgctctgaa
     9541 attccatttc gtacaggagt acccgtgcct tttgagatag agctatccat gctatcaaca
     9601 tcaaatgaaa catagatgtg agtgcaatgt gagagatgtg ccaatgcagt acggacaatt
     9661 ttctctactg ctatacgctt tacttcagca gcagaaatca tttttacatt atgttttttc
     9721 aaaagatatg cctcctgagg ttcaatatct cttacagcaa tatagactat atctttatag
     9781 gtaatcttag gcgacatccc tcctatcttc ttcaactttg tccaaaggtc aacagtttct
     9841 ttatcgggtt tgt
//
LOCUS       DAOOOL010000096         9786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_642_length_9786_cov_52.934334, whole genome shotgun sequence.
ACCESSION   DAOOOL010000096 DAOOOL010000000
VERSION     DAOOOL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_642_length_9786_cov_52.934334"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKU82_08450"
     CDS             complement(<1..314)
                     /locus_tag="PKU82_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ82942.1"
                     /translation="MRQLKITNMITIRENECLKRYLNEISQENLLTPNEEDELARRIK
                     TGDQQAMNKLVKSNLRFVVSVAKQYQNQGLTLEDLINEGNIGLIKAAQRFDSTKGFKF
                     IS"
     gene            642..723
                     /locus_tag="PKU82_08455"
     tRNA            642..723
                     /locus_tag="PKU82_08455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:676..678,aa:Leu,seq:tag)
     gene            783..2132
                     /gene="tig"
                     /locus_tag="PKU82_08460"
     CDS             783..2132
                     /gene="tig"
                     /locus_tag="PKU82_08460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOZ82943.1"
                     /translation="MNIQKEDVAPLNSLIRIKLTPEDYKTDVDSAIKKFKKSASMPGF
                     RPGHVPEGMIKKMYGKSFLADEINKIVSKSLYEFFRENNIAVLGEPLPKEVDHQNNFE
                     NPSDFEFLFEIGQAPAINLDLPSLPAVHYYQIEVDEKRVDTYIEDLQRKHGNFSNPET
                     SEESSILYGEFIELNENGEEKENGIRSTTTLAVNLVKDEASKAKLIGLKKDDEVTLDL
                     MKAMQDVTEVAYMLRVEKETAQQITSQFKYKIASINKVEKIEVGQELFDKVFGEGEIT
                     SVEDFRSKVKEDIAAMFARESMHKFNHDVEDALLHHLNISLPDDFLKRWIMEVNEKPL
                     TPEQIEQDYPAYSRQMKWKMIEGKLINDLQLTADEHEMAEYATAVIMQQFGQYQLGPD
                     MIADFAKRYLQTEENRNKADEAVKSRKVFDYLNQNVSKDVKEVSYDDFVTIVREHKH"
     gene            2155..2847
                     /gene="clpP"
                     /locus_tag="PKU82_08465"
     CDS             2155..2847
                     /gene="clpP"
                     /locus_tag="PKU82_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOZ82944.1"
                     /translation="MNYKNEFFKYATKHHGINSLAVGHYIESAVRAEGYISPTIIEER
                     QMNVASMDVFSRLMMDRIIFLGVPINDYVANIVQAQLLFLESTDAKKDIQIYMNSPGG
                     SVYAGLGIYDTMQYINPDVATICTGMAASMAAVLLCAGTKGKRTALKHARVMIHQPMG
                     GAEGQASDIEITAREIQKLKKELYEIIASHSGKDYDTVWKDSDRDYWMTAGEAKDYGM
                     IDEVLVRNKATQ"
     gene            2872..4110
                     /gene="clpX"
                     /locus_tag="PKU82_08470"
     CDS             2872..4110
                     /gene="clpX"
                     /locus_tag="PKU82_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOZ82945.1"
                     /translation="MKKQNEVKCSFCGRDKSATQVLIAGLNGHICDQCVTQAVEIISQ
                     ESTDQQKSKISSTFTLLKPAEIKKHLDDYVIGQDYAKKVMSVAVYNHFKRITGTTKKD
                     NDVEIEKSNIIMVGETGTGKTLLARTIAKILKVPFCIADATVLTEAGYVGEDVESVLT
                     RLLQAADYNVAAAEHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKMLESTIIN
                     VPPQGGRKHPDQKMIPVNTQNILFICGGAFDGIEKIIERRLQSQSIGYSAHRNQDAFD
                     KGNLYQYVSPADFKKYGLIPELIGRLPAISYLQPLDRAALRSILTEPKNALIKQYQKL
                     FEPEGIQLTFENEVLDFIVDKAIEFKLGARGLRSICETIMLDLMYELPQVKNKKDHVV
                     SLDYAREKVEKSNLGNLKVA"
     gene            complement(4182..4433)
                     /gene="purS"
                     /locus_tag="PKU82_08475"
     CDS             complement(4182..4433)
                     /gene="purS"
                     /locus_tag="PKU82_08475"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOZ82946.1"
                     /translation="MKFLAHIKVMPHKALLDPQGKTVSASMNNINLPTISNVRIGKHI
                     TCEVDAANQNEAKSMVDEACKKLLANPIMEYYEFELSEG"
     gene            complement(4442..5209)
                     /locus_tag="PKU82_08480"
     CDS             complement(4442..5209)
                     /locus_tag="PKU82_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOZ82947.1"
                     /translation="MALKNMIPNFITCLNLLCGCIGIVLCFTGHITGGSWMILLAAVF
                     DFSDGFAARMLKAQSAIGKDLDSLADVITFGLLPSIVMMQLFITVNPFMHTNAGLPFP
                     QMLLPWAPLLLAVFSALRLAKFNNDTRQTNSFIGLPTPANAMAIAAFPTLVQNYLVNN
                     TTGNSSLMENSIYQLFLHPTFYILFIIFMSWLLVAEIPLFSLKFKNFTLRDNLFPYIL
                     IAAAVLLFIFFKIGALPLVIILYVLLSVIKNSVATSK"
     gene            5388..6461
                     /locus_tag="PKU82_08485"
     CDS             5388..6461
                     /locus_tag="PKU82_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOZ82948.1"
                     /translation="MKIVTLTWCCLILSIASVAQTAPKYSNEFLNLGVGGRALGMGGA
                     QVSQVNDVTAGYWNPAGLVHSRGDIQVSFMHNEYFAGIAKYDYLALTAPIDTMRRIGF
                     SVLRFGIDDIANTIDLKDNDGNIDYNRVTGFSAADYAFLVSYASKSAISGLDYGANFK
                     IIHRKVGDFAKAWGFGLDAGIQYQAGNWRTGAMLKDVTSTFNAWSYTLNDRTKEVFIQ
                     TGNEIPENGLELTLPRLILGVSRQFNIKKFTITPALDLETTFDGKRNTLVKTNFASID
                     PRVGIEGGYNNLVYVRAGVSNFQQVTDIDGKKTTTVQPNIGVGIKLRNLSIDYALTNI
                     GNSNYLYSNVFSLRLTVNKLKKT"
     gene            6458..>9786
                     /locus_tag="PKU82_08490"
     CDS             6458..>9786
                     /locus_tag="PKU82_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOZ82949.1"
                     /translation="MRKLYVIIVLFLLPVLSFAQFSNSWINFNGASPYSAQQYFKIKV
                     WKQGIYRIDYSLLQQAGFPVGMNPNQFQIFNNGHEQYIYVEGAQDNSFDPTDFIEFYG
                     KPNNGAADQELYDDPSDQLNPDYSLFCDTAVYFLTAAILPGTPVKRMIMETDVNFSLY
                     MPENYVTKTFRRNYTSKYLKGDSQGDIDPSYNGPEGFSGNTFSAAAPADNFSLPNVST
                     FATAPIPSADVRMVNANYDGHFHTFNVEFNGNTIFTDNTIFGYGRHDYNFNIPAASLP
                     LSNSLVFSGVANSGGTNLMSVTYFKLQYPHANSFNGELEPFQFFSVSNGGSKARVDFT
                     DFLNSDNSTRFIYIIAGDTVSKVTTVRTGNLLQALIPVNGGEKNCLLAPASAYYFSTS
                     DFYLSPENSDPDPQKFARFTNYSYNQPQYDYLIVSHKKLWSEAVNYRDFRAQTGFNPL
                     LVDIDELYDQFAYGIYKHPLAIKNFCRFSITAFNNKPDYLLLLGKAVYPEEVRVSPTA
                     DQLCLVPTYGNPPSDYMFSNKIVDTTFVPRLATGRIAAQNITDVTNYLEKAQAHEAAR
                     QNCPDDWLKHVLHFIGGNNGFEQQSIKAFMDQNEAAIRDTSMGCTVTNYYKISTDPIE
                     YIQAIELQARIDSGVSIMTFFGHAAGSTFDIATDIPQNWHNAGRYPVVFAQSCNVGDI
                     YSPSRLLNEDFVLLPQKGSVGFLAKPRLGNIQELGEYSVQLYRNVAYRDYDKPVGMAI
                     KHALDSMIQTYYGNNGMVYKSTALGMLWHGDPGLRLAPSLQPDYAVSQPSITFQPTEV
                     TSELADFKMNIAISNFGKATKDSVSVHVVRKFPDNSTSVMDTLIPYIPYRDTLQLTMN
                     VDVNKGTGLNYFDVFVDYAGAITECNEFNNRVLNVPLQIISTDITPVYPFKYAIVPDP
                     SVILKATTDNIYAAQKTYRFELDTTDAFNSPFLRFKTFTQTGGIVKWPLPFTCDSGRV
                     YYWRVSLDPSLYPTTFKWKESSFIYIPQKTGWSQAHFFQFKNDSYTNIVYDKPQRKWE
                     YENTQAELRVSAWDVPGQGITSDNVQLNNQLIATGGCNYRAIYAVVLDSISLQNWSTL
                     DFAGKFGNINQPPYYDSCQATV"
BASE COUNT         3129 a         1700 c         1998 g         2959 t
ORIGIN      
        1 tacgagataa atttaaaacc ttttgtagaa tcgaaacgtt gagctgcttt tatcagtccg
       61 atgttgcctt cattaatgag gtcttctaat gtcaaccctt gattttgata ttgtttggca
      121 acgctaacta caaatcgaag gttagattta acgagtttgt tcatagcctg ctgatcgcct
      181 gtttttatgc gtcttgcaag ttcgtcctct tcgtttgggg ttaataaatt ttcctgtgaa
      241 atttcgttaa ggtagcgttt cagacattcg ttttcgcgaa tggtaatcat atttgtaatc
      301 ttcaactggc gcatggcaaa ttggttttta aaagttatac ttgattgggt gattttgtag
      361 taggcaacaa ccttgccaaa aaattcatgc aatccacatc tctgctgatt tttaggtgga
      421 gtttttattc acaatttgca atctaaatcg gtttttgatt tttttgtttt cgcaaagatt
      481 tcagaatgca gggaagcatt taaaaacagc tcccaataat ttcggtagta aaaaaaagaa
      541 aggttttttt tatttcagaa aagttcaaaa aaaaatgtag ttttgcacgc gttttataaa
      601 cggcattatt gaaaaataaa agctgtagat gaaatttccc agcgcatgtg gcgtaattgg
      661 tagccgcacc agacttagga tctggcgggg aaacccgtgg gggttcgagt ccctccatgc
      721 gcacgcaact gataaagctg aagctgtaca gcttcagctt attttatgta ataacttgta
      781 atatgaatat ccaaaaagaa gacgtagccc cgttaaactc actcatcaga ataaaactga
      841 cgcccgaaga ttataaaacg gatgtggaca gtgctattaa aaaatttaag aaatctgctt
      901 caatgcccgg tttcagaccc ggacatgttc ccgaaggaat gattaagaaa atgtatggta
      961 aatcttttct tgccgatgag ataaataaaa tcgttagcaa atcactctat gagttttttc
     1021 gcgaaaataa tattgccgtt cttggtgagc cattgccaaa ggaagtagat catcagaata
     1081 attttgaaaa ccctagcgat tttgaatttt tatttgaaat aggtcaggct cctgctatta
     1141 acctcgattt gccttcgctg cctgctgtac attattatca gattgaagtt gatgaaaaac
     1201 gtgttgacac ctatatagaa gatttacaac gtaagcatgg gaatttttca aatcctgaaa
     1261 catctgaaga aagttcaatt ctgtatggtg aatttattga gctgaatgaa aacggtgagg
     1321 aaaaagaaaa tggcattcgc agtactacta cattggctgt taatcttgta aaagacgaag
     1381 cttcaaaagc aaaacttatt ggtttaaaga aagatgatga agtgacactt gacctgatga
     1441 aagctatgca ggatgtaacg gaagttgcct atatgttgcg cgttgaaaag gaaacagcac
     1501 aacaaattac ttctcagttt aaatacaaga ttgcatcaat aaataaagtt gagaaaatag
     1561 aagtaggtca ggagcttttt gataaagttt ttggcgaagg cgaaataact tcagttgaag
     1621 atttccgcag taaagtaaaa gaagatattg cagcaatgtt tgcacgtgag tcaatgcata
     1681 aattcaacca tgatgttgaa gatgcactgc tccaccattt gaatatttca ttgcctgacg
     1741 actttttaaa gcgctggatt atggaggtta acgagaaacc tcttactcct gaacaaatag
     1801 agcaggacta tccggcctat tcacgacaga tgaaatggaa gatgatagaa ggtaagctga
     1861 ttaacgacct tcagttgact gccgatgagc atgaaatggc tgaatatgct acagctgtta
     1921 ttatgcagca gttcgggcag tatcagttag gtccagatat gattgctgat tttgcaaaac
     1981 gttatcttca aaccgaagaa aacagaaata aagctgacga agctgtaaaa agcagaaaag
     2041 ttttcgatta tctgaaccaa aatgtaagca aagacgttaa agaagtaagc tacgatgatt
     2101 ttgttaccat cgttagagaa cataaacatt aataaataat caaccggaat caatatgaat
     2161 tataagaacg aattttttaa atatgcaaca aagcatcatg gcatcaacag ccttgcagta
     2221 ggtcattaca ttgaatctgc tgttcgtgca gaaggttata tttcgccaac aatcattgaa
     2281 gaacgacaga tgaatgttgc cagtatggat gttttttcaa gactgatgat ggacagaatc
     2341 atttttttag gtgtgcctat taatgattat gtagccaaca ttgtacaagc acagttgctg
     2401 tttcttgaat caacagatgc aaaaaaagat attcagattt atatgaacag tccgggaggt
     2461 tctgtttatg caggtttagg tatttacgac actatgcaat atataaatcc tgatgtggca
     2521 acaatctgta ctggtatggc agcatcaatg gcagcagtgt tgttgtgtgc aggaacaaaa
     2581 ggaaagcgca cagcattgaa acatgcaagg gtgatgattc atcagccaat gggcggtgcc
     2641 gaaggacagg cttctgatat tgaaattaca gctcgcgaaa ttcagaaatt gaagaaagaa
     2701 ctttatgaga ttatagcttc gcacagcggt aaagattatg atactgtttg gaaagactct
     2761 gaccgcgact actggatgac agcaggtgag gcaaaagatt atggaatgat tgatgaagtg
     2821 ttggtaagaa ataaagccac acaataattt ttaaaaacgt agtgtgataa agtgaaaaag
     2881 caaaacgaag taaaatgttc tttctgtggc agagataaaa gtgctacaca ggttctcatt
     2941 gcaggactta acggtcacat ctgtgatcaa tgtgttactc aggcagtaga aattatttcg
     3001 caggagtcaa cagatcagca aaagtcaaaa atcagttcta cttttacgct gcttaaacct
     3061 gcagagataa aaaagcatct tgacgattat gttattggtc aggattatgc aaagaaggtg
     3121 atgagtgttg cagtttacaa tcactttaaa agaattacag gtactactaa aaaagataat
     3181 gatgtagaga ttgaaaaatc gaatatcata atggtgggcg aaaccggaac aggaaaaact
     3241 ttacttgcac ggactattgc aaaaatttta aaagttcctt tttgtattgc cgatgctact
     3301 gtattaacag aggccggata tgtaggcgaa gatgttgaaa gcgtattaac aagattgttg
     3361 caggctgccg attataatgt agctgctgca gagcacggta ttgtttatat tgatgagata
     3421 gataaaattt cccgcaagag tgataatcca tccattacac gtgatgttag tggtgaaggt
     3481 gtgcaacagg cgttgttgaa gatgctcgaa agcaccatca tcaatgttcc gcctcaggga
     3541 ggacgtaaac atcccgacca gaaaatgatt ccggttaaca ctcagaatat tctgtttatc
     3601 tgtggaggtg catttgatgg tattgaaaaa attattgaac gcaggttgca atctcagtct
     3661 atcggatata gtgcacatcg taatcaggat gcttttgata aaggtaattt atatcaatat
     3721 gtttcccctg cagactttaa aaagtatggg cttattcctg aactgatagg gcgacttcct
     3781 gcaatttcat atctgcaacc attagaccgg gcagcattaa gaagtattct cactgagcca
     3841 aaaaatgccc ttatcaaaca atatcagaag ttgtttgaac ccgaaggtat tcaactgact
     3901 ttcgaaaatg aagtgcttga ttttattgtt gacaaggcaa ttgagtttaa gttaggtgca
     3961 cgtggactac gttctatctg cgaaaccatc atgcttgacc tgatgtatga attgccgcag
     4021 gtcaagaata aaaaagacca tgtggtgtca ctcgattatg cacgcgaaaa ggttgagaaa
     4081 tcaaacctcg gcaatttgaa agttgcctga tagtttacaa attcagaaaa atatatttat
     4141 ttgtcaaaag caaccaaagt gttgcttttg gtgtttttgt atcaaccttc tgatagctca
     4201 aattcgtaat attccataat cggattggca agtaattttt tgcatgcttc atcaaccata
     4261 gattttgcct cgttctgatt ggctgcatct acctcgcagg taatatgttt tcctatgcga
     4321 acattggaga ttgtaggaag attgatattg ttcatacttg cactaacagt tttgccttgc
     4381 gggtctaaca gtgctttatg tggcatgact ttgatatgcg ctaagaattt catttctatc
     4441 tttatttaga ggtggctaca ctatttttta tcaccgacaa aagtacatac aatatgatca
     4501 ccaaaggcaa tgcacctatc ttaaaaaata taaacaacag aactgcggca gcaattaata
     4561 tgtaagggaa aaggttgtcg cgtaaagtaa agtttttgaa ttttaatgag aacagcggaa
     4621 tctctgctac caacaaccac gacatgaaaa taataaacaa aatataaaat gtagggtgga
     4681 ggaaaagttg ataaatgctg ttttccatca ggctgctgtt gcccgtagtg ttgttcacca
     4741 aataattctg aaccaaggtc ggaaatgcag caatagccat ggcattggca ggagttggca
     4801 gaccgataaa actgtttgtc tgacgggtgt cattgttgaa ttttgctaac cgcaaagcag
     4861 aaaaaacagc taatagcaag ggtgcccaag gcaaaagcat ctgcggaaaa ggtagtccgg
     4921 cattggtatg cataaaagga tttacagtaa taaacaactg catcataact attgagggta
     4981 aaagtccaaa agttataaca tctgccaaag agtcaaggtc ttttccgatt gctgactgcg
     5041 cctttaacat gcgtgcagca aaaccatctg aaaaatcaaa aaccgctgca agcaaaatca
     5101 tccaactgcc tcctgttata tggcctgtaa aacataaaac aataccaata caaccacaca
     5161 gtaggttcag gcaggtaata aagttgggga tcatattctt caatgccatc ggttttttgt
     5221 tatgatttaa atgttagaaa attacatcct gacatgcttt ttattggatg gattactata
     5281 aatttggtgt aaatattaaa aggattttta aatacagaca tgtttggcaa agaaaaaaat
     5341 aaaattagtc atttatcgtt tggtataggt atccaaatca tcagtttatg aaaatagtaa
     5401 ccctcacatg gtgttgtttg attttaagta ttgcttccgt agcgcaaact gcccctaagt
     5461 acagtaatga atttttaaat cttggcgttg gaggcagggc attgggcatg ggcggtgcgc
     5521 aggtttcgca ggtaaatgat gtaacagcag gctattggaa tcctgcagga ttggtgcatt
     5581 ctcgtggcga cattcaggta tcttttatgc ataatgaata ctttgcaggc attgccaagt
     5641 acgattactt ggcgttgaca gcaccaattg acactatgag acgcattgga ttctctgtac
     5701 ttcgttttgg cattgacgat attgccaaca ccattgatct gaaagataac gatggaaata
     5761 ttgactacaa tcgtgttaca ggattttctg ctgccgacta tgcatttctg gtttcttatg
     5821 ccagtaagag tgccattagc ggattagatt acggagctaa ttttaaaatt atacatcgca
     5881 aagttggcga cttcgcaaag gcctggggtt ttggacttga tgcaggcatt caatatcagg
     5941 caggcaattg gcgcacaggt gcaatgttga aagatgttac ttcaaccttt aatgcatgga
     6001 gctatacttt gaacgatcgt accaaagaag tgtttattca aacaggcaat gaaattccgg
     6061 aaaatggttt ggaactgaca ttaccacgat taattcttgg tgtttcacga cagtttaata
     6121 ttaagaagtt taccatcaca cctgcattag acttagagac aaccttcgat ggtaaaagaa
     6181 atacattggt gaagacaaat tttgcaagca tagacccaag agttggtatt gaaggcggtt
     6241 ataataatct tgtttatgtg cgtgcaggcg tgagtaactt tcagcaggta actgacattg
     6301 atggaaagaa gacaacaact gttcagccga atattggtgt tggaataaag ttgcgtaatc
     6361 tgtctattga ttatgcacta actaacattg gaaatagtaa ttatctttat tcaaatgtat
     6421 tttcattgcg attgacggtc aacaaactga aaaagacatg agaaaacttt acgtaataat
     6481 tgttttattt cttcttcctg ttttaagttt cgctcagttt tcgaacagtt ggattaattt
     6541 taacggagca tcaccttatt ctgcacaaca gtattttaaa attaaagttt ggaagcaggg
     6601 tatttaccgg atagattaca gcttgttgca gcaggcaggt tttcctgttg gaatgaaccc
     6661 taaccagttt cagattttca ataacggtca tgagcaatat atttatgttg aaggtgctca
     6721 ggataattca ttcgacccaa cagattttat tgagttttat ggtaagccaa ataatggtgc
     6781 agccgatcag gagttgtatg atgatccgtc tgaccagttg aatcccgact atagtttgtt
     6841 ttgtgatacg gcagtttact ttcttacagc agctatatta cccggcacac cggtgaaacg
     6901 aatgattatg gagactgatg taaatttctc tctttacatg ccggaaaatt atgttaccaa
     6961 aactttcaga agaaactata cctcaaaata tctgaaaggt gattcgcagg gtgatattga
     7021 tccatcatac aatggtcctg aaggattttc gggtaacacc ttttctgcag ctgcaccagc
     7081 agataatttt tcacttccca atgtttctac atttgctact gcgccaatcc ccagtgcgga
     7141 tgttagaatg gttaatgcca actatgatgg ccattttcat acatttaatg ttgagttcaa
     7201 tggcaacact atttttactg ataatacaat ttttggctac gggcgacatg actataattt
     7261 taacatacct gcagcatcat taccactttc aaacagtttg gtgtttagtg gcgtagcaaa
     7321 ttcaggtgga acaaacctta tgtctgttac ctattttaaa cttcagtatc ctcatgcaaa
     7381 ttcttttaac ggagaattgg aaccttttca gtttttctcc gtcagcaatg gtggtagtaa
     7441 agcaagagta gatttcacag attttctgaa cagcgacaat tcaacacgtt tcatttatat
     7501 aattgcaggt gatactgttt ctaaagtaac aacagttaga acaggaaatt tacttcaggc
     7561 tttaattccc gttaatggtg gagaaaagaa ttgcctgctt gctccggcat cagcatacta
     7621 tttttctact tccgattttt atttgtctcc cgaaaatagt gatcccgatc cacagaagtt
     7681 tgcacgtttt accaactaca gttacaatca gccgcagtac gattatttaa ttgtctcgca
     7741 taaaaaacta tggagcgaag cagtgaatta tcgtgacttc agagcacaga caggatttaa
     7801 tcctttgctt gttgatattg atgaattgta cgatcagttt gcttatggaa tttacaagca
     7861 tccgttagca ataaaaaact tctgccgttt ttctatcact gcatttaata ataaacctga
     7921 ttatttattg ttgttaggta aggcagtata tcctgaagag gttagagtaa gtccaacagc
     7981 cgatcaactt tgtttggtgc ctacctatgg taatccgcct tcagactata tgttcagcaa
     8041 taaaattgtt gacacaacct ttgtgccccg gcttgcaaca ggccgaatag ctgcacagaa
     8101 tataactgat gttaccaact accttgagaa agcccaggca catgaagctg caagacaaaa
     8161 ctgtcctgat gattggttga aacatgtttt gcattttatt ggaggaaaca atggcttcga
     8221 acaacagagc atcaaagctt ttatggatca gaacgaggca gccatcagag atacatcaat
     8281 gggttgtacg gttacaaatt attataaaat atctactgac ccaatagagt atattcaggc
     8341 aatagaatta caagcacgta ttgacagtgg tgtcagcatt atgacatttt ttggacatgc
     8401 cgcagggtca acttttgaca ttgcaaccga tattccacag aactggcata atgccggacg
     8461 ctaccctgta gtttttgcac aatcttgtaa tgtgggcgat atttattcgc cttcaagatt
     8521 gctgaacgaa gattttgttc tgcttccaca aaaaggatca gtaggatttt tagcaaaacc
     8581 ccgactggga aatattcagg agttgggaga atattctgta caactatacc gtaatgtggc
     8641 atacagagat tatgataaac ccgtaggcat ggcaataaaa catgcattgg attctatgat
     8701 acagacttat tatggaaata atggaatggt ttataaatct actgccttgg gtatgctgtg
     8761 gcatggtgat cccggattga gattagcacc ttcattgcaa cccgactatg cagtttcgca
     8821 gccatcaata acatttcagc ctacagaagt aacatctgaa cttgccgatt tcaaaatgaa
     8881 tattgcaata agtaattttg gtaaagccac gaaagattct gtttcagtgc atgtggtacg
     8941 aaaatttcct gataacagca catcagttat ggatacattg attccttata taccatacag
     9001 agacactttg caactgacta tgaatgttga cgtgaataaa ggaacagggc tgaattattt
     9061 tgatgtgttt gttgactatg caggtgctat taccgagtgt aatgaattta ataacagagt
     9121 gcttaatgtg cctttgcaaa taatttctac tgatatcact cctgtgtatc catttaagta
     9181 tgctattgtt cctgaccctt cggtaatctt gaaagcaaca acagataata tttatgctgc
     9241 acaaaaaact tatcgttttg aattagatac aaccgatgca tttaattcac cttttctaag
     9301 attcaaaacg tttacacaaa caggaggcat tgttaaatgg ccattacctt ttacatgcga
     9361 cagtggcaga gtttattatt ggcgtgtaag ccttgaccca tcactttatc caacaacatt
     9421 taaatggaaa gaaagttctt ttatttatat tccacagaaa acaggttgga gtcaggcaca
     9481 tttttttcaa tttaaaaatg acagctacac caacattgtt tatgataaac cacaacgtaa
     9541 gtgggaatat gaaaacacac aagcagagtt gcgtgtatct gcatgggatg tgcccggaca
     9601 aggaattacc tctgacaatg tgcaactcaa taatcagttg attgcaacag gtggctgtaa
     9661 ttatcgtgct atttacgcag tggtacttga ttctatttct ttacaaaact ggagtacact
     9721 tgattttgca ggaaaatttg gtaacatcaa tcaacctcct tattacgatt cttgtcaggc
     9781 aacagt
//
LOCUS       DAOOOL010000097         9750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_646_length_9750_cov_52.861372, whole genome shotgun sequence.
ACCESSION   DAOOOL010000097 DAOOOL010000000
VERSION     DAOOOL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_646_length_9750_cov_52.861372"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..497)
                     /locus_tag="PKU82_08495"
     CDS             complement(78..497)
                     /locus_tag="PKU82_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOZ82950.1"
                     /translation="MADIFSKRKRSEIMASISGKETKPEISVRKFLFKEGFKFRKNVK
                     TLAGTPDIVLPKYQTIVFVHGCFWHGHNCNKAKLPTSNAKFWKTKIQNNIERDKKVKA
                     ELKKSGWTVLTIWECKLKSNKLFENSMNKLREELNAK"
     gene            complement(490..2235)
                     /locus_tag="PKU82_08500"
     CDS             complement(490..2235)
                     /locus_tag="PKU82_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ82951.1"
                     /translation="MKARNAFRNFIEYIQFPYFRNLEKNTRIIFDFPLTVFVGQNGSG
                     KSSTLHALYGAPKKNTPFEFWFSTAVDPIVEVSEGGDRHSFFYVYKDNRGRPLEVLKQ
                     RIFKRNNPDYWETARPNISYGMQKIAEGKRNQPIEKDVVYIDFRSELSAFDKYFYFKK
                     PRKSKTINNKQDYLRKHSVFLRRIIDGTNVIIHRYGENKNEQLKTFTAIELQHISTIL
                     GKKYVSGKFVKHKLFEEWGYSVIFETASYSYSEAFAGSGEMAVVKLVLEVLGAKEYSL
                     ILLDEPEVSLHPGAQKRLKQFLLEETRRKRLQVVISTHSPSIIEELPPNAIKVFSLLP
                     SGKFNVKNEITPDEAFYHLEQTNPARKTIIVEDLLSKQIIDGVLRELGQATASLFNVT
                     FYTGGESVIKEHFISVYSHANERNKFIVFDGDQRHAHFNPDSIVAAEQTNERYQQEIQ
                     NQTGVNVKFYPDGNPNAGARADQEKVLREKYLKFYLRNVSYLPLSVPEEIIWNQVLAE
                     TILTAMGKIEYIQRINATQDFKEKFLLVAEAMTGDKRNIEASEKTFLEKWLSDKPNSY
                     NDIVTIIESIRALNG"
     gene            complement(2277..3905)
                     /locus_tag="PKU82_08505"
     CDS             complement(2277..3905)
                     /locus_tag="PKU82_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HOZ82952.1"
                     /translation="MSNDKLYMTFEANTIKHLGVKMYSNMPPALAELIANAYDACATE
                     VHVRLYDEGEKKVIVEDNGTGMSFAEVNDYFLRIGRNRREEGQKSSCDRIPTGKKGLG
                     KLALFGIGDTINITTCQGKSGVSFNLDWNEILAWKGKDYEPRFDHLSEKEIFKGTRIT
                     LTNLKRKTGFPLEEYAESIAKLFNFQDKFIVYLLLNDSKPIKIDNKLKYDNITPEFEW
                     DIQEIIKVNEKDYSDKVQIHGTIITTEKPLKAALRGVTLFANGRMVNTPEFFGPSESS
                     HFYSYATGWLNVDFIDNWEEDVISTNRQSIDWENAKTSALRKYIASCLSIIERQWREF
                     RKEKKKKKISEKVNLNVKDWLEKLPQNVQGQVETIVNLLDDTPEISEDTQQVVTRLLH
                     DIAPEYANLHWRNLENEIKVVSEKYYQSADYYTAFIEALKRYTSEVRKKSGSTNGSER
                     SMMGEVFSGRKLSVTKKYKKTDGTDFTKDTITNIEEGQHYLSEGIVAGGRNPLQHEEH
                     LELSKTGLFSEKDCLDFLSLLSHLFKRLNDSEKP"
     gene            complement(3902..4978)
                     /locus_tag="PKU82_08510"
     CDS             complement(3902..4978)
                     /locus_tag="PKU82_08510"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOZ82953.1"
                     /translation="MKGKKSKTKRKFKAVDFFCSGGGMTSGFRKAGVNVVGGIDIDLE
                     CKETYTTNNPDSKFIHADIQDLAFSRIEKELKVKRNDKKMIFIGCSPCQYWSKIQTDK
                     TKAHETKNLLKDFQRFVDYFKPGYIVVENVPGILNRLDESPLKSFLDFLEKENYHYKY
                     KVINASHYGVPQTRRRFLLIASRVTDKISLPLPDTKTKLPTVRNFIGNKKVFKPITAG
                     QKDKTEFLHSSAGLSDNNLKRLLLTPHDGGTRTSWKDTDLQIPAYVGKDNSFQDIYGR
                     MFWDKPGPTITTKFYSITNGRFAHPEQNRAISLREGATLQTFDTDYKFLSTSIASTAR
                     LIGNAVPPQLSMRVAASIINCNPK"
     gene            complement(5477..6067)
                     /locus_tag="PKU82_08515"
     CDS             complement(5477..6067)
                     /locus_tag="PKU82_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82954.1"
                     /translation="MKKLIPITLISIVFFLSSCGTGKKSVSTTTTTTAVTPGFKITAP
                     DSITKTPVSAMITPAYTTTIHSSNFIVESASAWGAYEFTQAGDYKIVIMDVGSKTDEG
                     EKEFVNFCLEGQAVNKHFEDLKIIRPEVTNGGRCINGLAQNLVSNEYVVRGIKPGDQI
                     KAWPSRNGATMRRTGLNGTQAEWDGDGRLKVLITPL"
     gene            complement(6792..>9750)
                     /locus_tag="PKU82_08520"
     CDS             complement(6792..>9750)
                     /locus_tag="PKU82_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOZ82955.1"
                     /translation="IYSQNVFFPANSGFLRSPFILRDFRGQTVVVNPFSYNAATQTLK
                     VISKFKVTLQSKAGTSVNTLSRTTSLQKVDRDFANIYQNVFRNAGSFINYLPVNEDGE
                     MLIISDASFMAAMQPFIDWKIKRGLQVSMVDVATAGSTAQQIKTFIQNYYSTHNLKYV
                     LLVGDAQQIPTPVLSGGASDPSYGYLVGNDSYAEVFVGRFSAQTVADVTTQVQRTVDY
                     EMLPTPGVNWYSKGICIGSDQGPGDDNEMDFQHQQVIRGKELAFTYTDVAELYDGSQG
                     GNDMSGNPTAAMLSSEINNGASIITYTGHGSSASLGTTGFSNANATALTNTGMLPFIW
                     SVACVNGEFANATCLAESFLRAQYNGQPSGAVATFMSSINQSWNPPMDAQDEMVDLLV
                     GTNSSNIKRTFGGLSMNGCMHMNDQYGAAGFEMTDTWHCFGDPSLNVRTATPYAITAS
                     HSPVLFPGASQLNIACNTDSALACLSSNGQILATGLVIGGNVILNFNTITSLDTLYLT
                     ITAFNGIPYMVAIPVVATTGPYVSLTSNVINDAGGNANQLADYNEIIDVDLMLENFGQ
                     GDAVGVGLTVTTTCPYVTINSSSLNKVNIQSGNAAGITNAFNYTVAAFVPDNTPAVFD
                     IVLTDSAGTVWSTSITHLLKAPMLQVYSITVSDPTGNNNGILEPGETADVTIKTNNKG
                     HSATTASTATLATSSPYLTINNTSYNTGVVQALSDVDATFNVSLASNIQIGSTFDMQF
                     NIDANPYSDNKTFNMVAGEILENFEYGNFTTYPWLNNGSHPWTITNSGTFQGAYSARS
                     ATIADGDTSDLAITVNVYNNDSISFYMLISSEMGWDFLSFYIDGTLIDSYSGVYGWAY
                     KSYPITLGQHTLTWAYIKDEVCCTGGQDLAMLDNIQFPPSTMVTSAKDFSSINSVTVY
                     PNPAQDELTIITKDFKNVEVKLFAMDGKLVYSAANIISDVYKMPLNDISQGMYYLQIK
                     ADNKFYNSKIIKR"
BASE COUNT         2733 a         1904 c         1662 g         3451 t
ORIGIN      
        1 atttttttat ccatgtttta cactttttag tgtaaaccta tttcaggaca agacatgtgt
       61 gtgcttttgt gtgttggcta ctttgcattt agttcttccc ttagtttatt cattgagttt
      121 tcaaacagtt tattgctttt caatttacat tcccaaatag ttaatactgt ccaacctgat
      181 ttttttaatt ctgcctttac ttttttgtct cgttcaatat tgttctgaat ttttgttttc
      241 caaaattttg catttgaagt tggcaacttt gccttgttgc aattatgtcc atgccaaaaa
      301 catccatgaa caaaaactat cgtttgatac ttgggtaaaa ctatatctgg tgtccctgca
      361 agagttttta catttttccg aaacttaaat ccttctttaa acaaaaactt tctgactgat
      421 atttccggct tagtttcctt cccggaaatt gaagccataa tttcacttcg tttccgttta
      481 gaaaaaatgt cagccattta atgcacgaat tgattcaata attgttacaa tatcgttgta
      541 actatttggt ttatcactca gccacttttc taaaaatgtt ttctcacttg cttcaatatt
      601 tctcttatca ccagtcattg cctccgcaac taaaagaaat ttctctttga aatcttgtgt
      661 agcgttgatt ctttgaatgt attcaatttt acccatcgca gtcaaaatag tttcagctaa
      721 aacttgattc caaataattt cttctggaac tgataatggt aagtaggaaa catttcgcaa
      781 ataaaatttc agatactttt ctcgcaacac tttttcttgg tctgctcttg cccctgcatt
      841 tggatttccg tcaggataaa acttaacatt gactcctgtt tgattttgga tttcctgttg
      901 gtatcgttca ttggtttgtt ctgctgctac aatagaatca ggattaaagt gagcatgtct
      961 ttggtctccg tcaaaaacta taaacttatt ccgttcgttt gcatgactgt aaacactaat
     1021 gaaatgttct ttaataacgc tttcacctcc cgtgtaaaaa gtaacattga aaagtgaagc
     1081 agttgcttgt cctaattctc ttaatacacc atcaataatt tgctttgaaa gcaaatcttc
     1141 aacaatgatt gttttccttg ccggatttgt ttgctccaaa tgataaaacg cttcatcagg
     1201 tgtgatttca tttttaacat tgaattttcc agaaggtaat agtgagaaaa cttttattgc
     1261 gtttggcgga agttcctcaa taatacttgg tgagtgggtt gagattacaa cttgtaatct
     1321 ttttcgtctt gtttcttcaa gcaaaaattg cttcaatcgt ttttgcgctc ccggatgcaa
     1381 tgaaacttcc ggctcatcaa gtaaaatgag agaatattct tttgctccta aaacttcaag
     1441 caccaacttt acaactgcca tttctccgct tcctgcaaac gcttcggaat atgagtaact
     1501 ggcggtttca aaaattacgg agtagcccca ttcttcaaaa agtttatgct ttacaaactt
     1561 tcccgaaaca tattttttac caagtatggt agaaatatgc tgaagttcaa ttgctgtgaa
     1621 agttttgagt tgctcgtttt tgttttcacc gtagcgatga attataacat ttgttccgtc
     1681 aataattctt cgaagaaaaa ctgaatgctt ccgtaagtag tcctgtttgt tatttatcgt
     1741 tttagacttt cttggtttct tgaaataaaa gtatttgtca aatgcactta actctgaacg
     1801 gaaatcaata tacactacat ctttttcaat cggttgattt ctttttccct ctgctatctt
     1861 ctgcattccg tagctgatat tcggtcttgc agtttcccaa tagtccggat tgtttcgttt
     1921 gaaaattctt tgtttcaaaa cttcaagcgg tctgccacga ttgtctttgt aaacataaaa
     1981 gaagctatgc ctatcgcctc cttccgaaac ttctacaatc gggtcaacag cagttgaaaa
     2041 ccaaaattca aacggagtat tttttttcgg tgctccataa agtgcatgaa gtgttgaact
     2101 ttttcccgaa ccgttttgcc ctacaaaaac tgtcagagga aaatcaaaaa ttattcgtgt
     2161 gtttttttca aggtttcgga aatatggaaa ctgaatatac tcaatgaaat ttctgaaagc
     2221 atttcttgct ttcattcttg aaatttcagc tatgagagtt ggtatattca caaatgctat
     2281 ggtttttcag agtcgttaag tcgtttgaat aaatgagata acaaactaag gaagtccaaa
     2341 caatcttttt ctgaaaacaa acctgtttta cttaactcaa ggtgttcctc gtgttgcaaa
     2401 gggtttctgc cgcctgcaac aataccttcg gatagatagt gttgtccttc ttcaatattg
     2461 gttattgtat cttttgtaaa atctgttcca tcagttttct tatacttttt cgttaccgaa
     2521 agtttacgac cagaaaaaac ttctcccatc atacttcttt cgctgccatt tgtgctacct
     2581 gacttctttc ttacttctga ggtatatcgt tttaatgctt caataaaagc cgtgtaatag
     2641 tcagcactct gataatattt ttcagacacc actttaattt cattttctaa atttctccaa
     2701 tggagatttg catattcagg ggctatatca tgcaataatc ttgtcaccac ctgttgtgtg
     2761 tcttctgaaa tttcaggtgt gtcatccaat aggtttacaa ttgtttcaac ttgcccttgc
     2821 acattttgag gaagtttttc taaccaatct tttacattca agtttacctt ttcacttatc
     2881 ttctttttct ttttctcctt acgaaattca cgccactgcc tttctattat tgaaaggcat
     2941 gatgcaatat atttcctgag tgcggatgtc ttggcatttt cccagtcaat ggattgacgg
     3001 tttgttgaga ttacatcttc ttcccaattg tcaataaaat ctacattcaa ccatcctgtg
     3061 gcataagaat aaaagtggct tgattctgat ggaccaaaaa attcaggtgt gttaaccatt
     3121 ctgccgttag caaaaagtgt aacgcctctg agagcagctt tgagaggttt ttctgtggta
     3181 attattgttc catgaatttg aactttgtcg gaataatcct tctcatttac tttgataatt
     3241 tcttgtatgt cccactcaaa ttcaggagta atgttatcat acttcagttt gttatcaatc
     3301 tttatcggct tgctatcatt cagcaataag taaacaataa acttgtcttg aaagttgaaa
     3361 agttttgcaa tgctttctgc atattcttca agaggaaatc cggtttttct tttcagattg
     3421 gttaatgtga ttcttgttcc tttgaaaatt tccttttctg ataaatggtc aaatctgggt
     3481 tcataatctt tccctttcca agcaagtatt tcattccagt caagattgaa acttactccc
     3541 gattttcctt ggcaagttgt aatgtttata gtgtcgccaa ttccaaatag agctaacttt
     3601 cctaatcctt tttttcctgt tggaattcta tcgcaacttg atttttgacc ttcctctcgt
     3661 ctgtttcttc ctattcttaa aaagtaatca ttcacttccg caaacgacat tcccgttccg
     3721 ttatcctcaa caattacttt tttctcccct tcgtcataca aacgcacatg aacctctgta
     3781 gcacacgcat cataagcgtt tgcaattaat tccgctaatg ccggtggcat gttggaatac
     3841 attttaacac caagatgttt tatggtgtta gcttcaaagg tcatgtaaag tttatcattg
     3901 ctcatttcgg attacaattt atgatgcttg cagcaacacg cattgataat tgcggtggaa
     3961 ccgcattgcc aatcaatctt gctgttgatg caattgaagt agaaagaaat ttgtaatcag
     4021 tgtcaaaagt ttgcagcgtt gcaccttctc ttaatgaaat ggctctgttc tgttctggat
     4081 gagcaaatct tccgttggtt atactataaa actttgttgt gattgttggt ccaggtttgt
     4141 cccaaaacat tctaccatag atgtcttgaa atgagttgtc tttgccaaca taagccggaa
     4201 tttgcaagtc agtatctttc cacgaagttc ttgttcctcc gtcatgtgga gttaagagca
     4261 atcgctttaa attattgtct gatagtccgg ctgaactgtg aaggaactcc gtcttatctt
     4321 tttgccccgc tgtaattggt ttgaaaacct ttttattgcc aatgaaattc cgaactgtcg
     4381 gcagctttgt ttttgtgtcg ggaagtggca aagatatttt gtcggtaact cgtgatgcaa
     4441 tcaacaggaa tcttcttctt gtttgtggca ctccgtaatg gctggcatta attactttgt
     4501 atttgtagtg atagttttct ttttctagaa agtcaagaaa tgatttcaaa gggctttcat
     4561 ctaaacgatt taaaatccct ggaacatttt caaccactat gtaaccaggt ttgaagtaat
     4621 caacaaatct ctgaaagtcc ttcaataagt tttttgtttc atgtgcctta gttttatcgg
     4681 tttgaatctt gctccagtat tggcatgggc tgcacccaat gaaaatcatt tttttgtcat
     4741 tgcgttttac tttcagctct ttttcaattc ttgaaaaagc taagtcctga atgtcagcat
     4801 gtataaattt tgaatcggga ttattagttg tatacgtttc tttacactct aaatcaatat
     4861 caattccgcc aacaacgttt actcctgcct tccgaaaacc gcttgtcatg ccgcctccgg
     4921 aacagaagaa atccacagct ttaaattttc tttttgtctt tgatttttta cctttcataa
     4981 acggctgcga atttatgatt ttcactctta aattcatacg aaatcgcaat aaagcgttgg
     5041 caaaaagtgg ctctttttgt tttgcctctg tggtgggtcg tgtgttgcgg caaaacaaaa
     5101 tgtgccactt gtgcggttgc catagaaatc attttttttt gtgcgttggc ggtgttttaa
     5161 tttttttgaa gcggttgcct gttggttaga agtgctgtca taattattac ttgtcatctt
     5221 aaatgtttgt tcaaagtccg tccgtttaat gtccgcatgg tcgcaaatag agttaccgat
     5281 aacgaagaag ggattaacga agtttaaaaa agaaggagat agaaagcaca cactctccgc
     5341 accgatacgc tttattacct gcactcactt tcaagtggct gtaatgccct tcttttttaa
     5401 atttcgttta atctattgtt atgcgttcgt gctacgatct acacgaagag tcagatctat
     5461 attcacttat tataaattac aatggtgtta ttaaaacttt taatcttcca tcaccgtccc
     5521 attctgcttg agttccgttt agtcctgttc ttctcattgt tgctccattt cttgatggcc
     5581 atgctttgat ttggtcacct ggtttaattc ctcttacaac atactcattg cttacaagat
     5641 tttgtgcaag accatttatg caacgtcctc catttgtaac ttctggtctt ataattttca
     5701 aatcttcaaa atgtttattt acagcttgac cttctaagca gaaattaaca aattctttct
     5761 caccttcatc tgttttagac ccaacatcca tgatgacaat cttgtaatca cccgcttgag
     5821 taaattcata agcaccccaa gcacttgcag attctacaat aaaattagag gaatggatcg
     5881 tggttgtata ggcaggtgtt atcattgcag agacaggtgt tttggttata gagtcagggg
     5941 ctgttatttt aaagccaggt gtcactgctg tggttgtggt cgtagtggaa actgattttt
     6001 ttccggtgcc acaggatgat aagaaaaata ctattgagat aagagtaatg ggtattaatt
     6061 ttttcattgc atgtaaattt attttgtttt gcttactctt tgacggattt tcagcttact
     6121 ccatattata tattggtgtc aatgtttatt tcttccgatc actttcaaaa ttagcactcc
     6181 ccccaagcat gacgcataac atttatatta tcaacactca caattcacaa atttattcat
     6241 tttgaaacaa atataaaaat tattcgcaac tcaaggtgcg gattatcgca actgtcaaaa
     6301 gcgtagtttg ccttaggaag tttgaatgtt tggcaagttg caaggtttag aaggttacaa
     6361 tgttttgaat gtttgaaggt tttgaatgtt acaatgtttt gaaggtttga gggtttttaa
     6421 aatgttgttg ccttgaactt gcaatgtcta aattgaattc tctttcaaaa ttttcaaact
     6481 tttcagccgt ggctaatttt caaacttgtc agccgttgct gattatctaa ttatcaaatt
     6541 ttcaaattac ctcagtatta tttactccat cactcctatc agccgtggct aattttcaaa
     6601 ttatctaatt atcaaatttt caaattacct cagtattatt tacttcatca cttctatcag
     6661 ttgtggctca gccgtggctg attatcaaat catctcattt tcaaattatc aaactctctt
     6721 tatcattcat aaaaaaaaac accgaaatct aaaaaactga ttccggtgtt tttctaaacg
     6781 ggattattct tttaccgttt tataatcttt gaattgtaaa atttattgtc tgcttttatt
     6841 tgcagatagt acatgccttg tgagatatca ttcagcggca ttttataaac atctgaaatg
     6901 atattagctg ctgaatacac cagcttacca tccattgcaa ataatttcac ttcaacattt
     6961 ttaaaatctt tggtaattat ggttaactca tcctgagcag gattgggata aacagttaca
     7021 ctattgatag aactaaaatc tttagccgaa gttaccattg ttgatggtgg aaactgaata
     7081 ttatcgagca tagccaaatc ctgtccgccc gtgcaacaca cttcatcctt aatatatgcc
     7141 cacgtgagtg tatgttgtcc taaagttatc ggatatgatt tatatgccca gccatacaca
     7201 ccggaatagg aatcaatcaa tgtaccatct atgtagaaac tgagaaaatc ccatcccatt
     7261 tcacttgata tgagcatata aaacgaaata gaatcgttgt tataaacatt tactgttatt
     7321 gccagatcag aggtatcgcc atcagcaatt gttgcagaac gtgcagaata cgccccttga
     7381 aaagttccgc tgttagtaat agtccatgga tgacttccat tattaagcca gggatatgta
     7441 gtgaaatttc cgtattcgaa attctctaaa atctcccctg caaccatgtt aaatgtcttg
     7501 ttgtcggaat atgggttggc atcaatatta aattgcatat cgaaggttga accaatctga
     7561 atgtttgatg caagtgagac attaaaggta gcatcaacat cagataatgc ctgcacaaca
     7621 cctgtattgt acgaagtatt gttgatagta agatatggac tgcttgttgc cagcgttgct
     7681 gttgaagctg tagttgcact atgtcctttg ttatttgtct ttattgtaac atcggcagtt
     7741 tcacctggtt caagaatacc gttgttgttt cctgtagggt cagacactgt aatggaatat
     7801 acttgcagca taggtgcttt caacaaatgt gttatggatg tactccacac tgtacctgct
     7861 gaatcggtta atacaatatc aaatactgca ggtgtgttat caggaacaaa agctgcaaca
     7921 gtgtaattaa atgcattggt aattccagca gcgtttccac tctgaatatt cactttgttt
     7981 aaactgctgc tgtttattgt aacatacgga caagtagtag ttaccgtaag tccaaccccc
     8041 acagcatcgc cctgtccaaa gttttcaagc attaagtcaa catctataat ctcattataa
     8101 tctgcaagtt gatttgcatt tcctcctgca tcattaataa cattgcttgt cagcgaaacg
     8161 taaggtcctg tagttgctac aacaggaatt gccaccatgt atggaatgcc gttaaatgca
     8221 gtaattgtta aatacagagt atcgagtgaa gttatcgtgt taaaatttaa aattacattc
     8281 cctcctatca ccaatcctgt tgcaagtatt tgtccattgc tgctcaaaca tgcaagtgca
     8341 ctgtctgtat tacaggcaat atttaattgt gaagcaccgg gaaacaatac agggctgtga
     8401 ctagcagtaa ttgcataagg tgttgctgta cgaacattta atgaagggtc gccaaaacaa
     8461 tgccatgtgt ctgtcatttc gaaacctgct gcaccatact gatcattcat gtgcatgcag
     8521 ccattcatac tcagtccgcc aaaagttctt ttaatgtttg aagaatttgt gccaaccaat
     8581 aagtcaacca tttcgtcctg tgcatccatt ggcggattcc atgattggtt tatgctcgac
     8641 ataaatgtgg ctactgcacc tgaaggctgt ccattatatt gtgctctcaa aaatgattct
     8701 gcaagacaag tagcatttgc aaactctcca ttgacacaag ctacactcca gataaaaggc
     8761 aacatgcctg tgtttgttaa tgctgttgca ttggcattag aaaaacctgt tgtgcctaat
     8821 gaagcagaac ttccatgacc agtataagtg attatagaag caccattatt tatttctgat
     8881 gataacattg cagcagtagg attacccgac atatcgtttc caccttgtga gccatcatac
     8941 aactcagcaa catcagtata agtgaatgct aattcttttc cgcgtatcac ctgctgatgc
     9001 tgaaagtcca tttcattgtc atcacccggt ccttggtcag agccaatgca tattcctttt
     9061 gaataccagt ttacacctgg cgttggcaac atttcataat caacagtacg ctgaacttgt
     9121 gtagtaacat ctgccactgt ctgtgcactg aatcgtccaa caaatacttc tgcataagaa
     9181 tcattcccca caagataacc atacgatggg tcagatgcac cacccgacaa aacaggagta
     9241 ggaatttgtt gtgcatcgcc taccaacaaa acatatttta aattatgtgt gctgtaataa
     9301 ttctgaatga atgtttttat ctgttgtgct gtgctgcctg ctgtggctac atcaaccatt
     9361 gaaacctgca agcctctttt tatcttccag tcaataaagg gctgcatagc tgccataaaa
     9421 gatgcatcac tgataatgag catttcacca tcttcattca ccggtaaata attaataaat
     9481 gaacctgcat tgcgaaaaac gttctgatag atatttgcaa aatcacgatc aactttctgg
     9541 agtgatgttg tgcgagataa tgtattaaca gaagtgccgg ctttactttg cagggtaact
     9601 ttaaattttg aaattacttt taaagtttgt gttgctgcat tatagctgaa tggatttact
     9661 accactgtct gcccacgaaa atcacgaagt ataaacgggc tacgcaaaaa gccactgtta
     9721 gcaggaaaaa aaacattctg actgtaaata
//
LOCUS       DAOOOL010000098         9734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_647_length_9734_cov_200.945862, whole genome shotgun sequence.
ACCESSION   DAOOOL010000098 DAOOOL010000000
VERSION     DAOOOL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_9734_cov_200.945862"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="gcvT"
                     /locus_tag="PKU82_08525"
     CDS             complement(<1..945)
                     /gene="gcvT"
                     /locus_tag="PKU82_08525"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOZ82956.1"
                     /translation="MEENTTLKHTALTAIHQKAGAKMVPFAGYLMPVQYTGINDEHET
                     VRNAVGMFDVSHMGEFILKGDDALDLLQRVTSNDASKLSAGKAQYSYFPNETGGIIDD
                     LIVYCIDEKTFMLVVNASNIEKDWNWLQKHNNKKVEMHDISDKTSLLAVQGPKAMETL
                     QKLTSIALADIPYYNFAKGEFAGCQNVLISNTGYTGAGGFELYFENQYAEKIWNDVMQ
                     AGQPFDIKPAGLGSRDTLRLEMGFCLYGNDIDDNTSPLEAGLGWITKFTKNFTNSDAL
                     KQQKEQGIANKLVGFEMIDRGIPRHDYEICNASGQKIGR"
     gene            1063..2556
                     /locus_tag="PKU82_08530"
     CDS             1063..2556
                     /locus_tag="PKU82_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOZ82957.1"
                     /translation="MKLLTSEQFRMADAYTIKTEPISSVDLMERAAICCSDEIAKFTD
                     EKDHICILCGPGNNGGDGLAIARILADAGKNVSVLLTDNSNKLSPDAEANFLRLQKTS
                     VKIFYLTDANLPFKNTTFWVDAIFGTGLNKAVEKNIADLIHQVNSTRTPVISIDLPSG
                     LFADKHTPTNVAVIHASTTLTFQRLKRSMLYAENAHRIGEVKVLDIGLDEHYMESLPT
                     TQELVDRSLINKILKPRKRFSHKGNFGHALLIGGSYGKAGAIILSAKACVKSGAGLTT
                     VHTVKTTGATLQSAIPEAMLSTCEGDKVCDLPDLAPYNAIAAGPGLGINEQTSSMLKL
                     LLQKAKKTLVLDADALNIISQNKNLIDNLPENTILTPHKKEFERLFGTFENDFQRNDF
                     QIAASVKHKIIIVLKGAYTSISTPEGKCFFNTTGNAGLAKGGSGDTLTGIITALLAQG
                     LNATDAAIAAVYIHGLAADCAVKKIHPYSLTASDVTEFLSEAFIQTV"
     gene            complement(2540..4546)
                     /gene="ispG"
                     /locus_tag="PKU82_08535"
     CDS             complement(2540..4546)
                     /gene="ispG"
                     /locus_tag="PKU82_08535"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOZ82958.1"
                     /translation="MDSALTDQNIFQLYCNSLTSYQRLKTLEVKIGDVAMGANHPIRV
                     QSMTTTDTLDTKATVEQSIRMIVAGCEFVRITAPSINEARNLQNIKDELRKRGYKTPL
                     IADIHFTPNAAETAARIVEKVRVNPGNYADKKKFDTIEYTDVTYAEELERIRERFIPL
                     VKICKEYGTAMRIGTNHGSLSDRIMSRYGDTPLGMVESALEFLRICMDEGYYSIVLSM
                     KASNPQVMVQAYRLLIAKMRAEFMDLNNMQNTLPALFPLHLGVTEAGEAEDGRIKSAS
                     GIGTLLEDGIGDTIRVSLTEDPELEIPVAQKLVSRYNNRTSHQTIKPINHHKLPYSPF
                     SYSKRLTKAVLNVGGNHVPVVIADFSGKQNISPASLFPVGYNYSQSLDKWHISDQACD
                     FIYVGNQHISFALPGTLGVIYNNKMWLQHSDDERTYPLFNVNELNDALKCTAAIKFIE
                     LNTEDFFKQQLQVDKLSDAVLVLKTSNDHAMPDLRNVFVDMMQQKNTLPVIIKRDYAK
                     ADLETFRLHCSTDAGALLLDGMGDGVWLCSELVSPADINQTAFGILQAARTRISKTEY
                     ISCPSCGRTLFDLQETTAKIRARTHHLKGIKIGIMGCIVNGPGEMADADYGYVGSGAG
                     KITLYKGKEVVRRNIDAALAVDALIELIRDNGDWIEPAVITPSV"
     gene            complement(4619..5527)
                     /locus_tag="PKU82_08540"
     CDS             complement(4619..5527)
                     /locus_tag="PKU82_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82959.1"
                     /translation="MKKIIFVLLIACSHFVAKAQFAPVGAQWHYTQCTVTFPYSCGPV
                     EVNATGTTVINGITCTNLQIAPFTCVGFNYLPVYQSNDTVYRFLYNLNTFTMLYDFNA
                     QPGDIWNIVSNTINQPPPAPLTDTVRIRVDSVNMVNINNHPRKIFYISYADSSHIPFV
                     FEGPIIEGIGSLNFLLTQFNLCDPQITAFRCYNDSTLGLYQPNTLVDCDSVVVVGESE
                     VIKKDYVSLYPNPFTDQICIHSEDFLYRKYDIKLVNIVGKVVYHSKQRQALDQLTIYP
                     KNLAPGTYYLLLESDKKVLQKRVVKL"
     gene            complement(5569..6657)
                     /locus_tag="PKU82_08545"
     CDS             complement(5569..6657)
                     /locus_tag="PKU82_08545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82960.1"
                     /translation="MSHNKKKILLICPYPEDTAPGQRLRYEQYLNLFRKEGYDIHVSS
                     FMTYRFNSIVYKPGNVPEKIFWTLFGYIKRFLQLFTLWRYDAVYIFLWVTPFGLPLFE
                     WLFVLLNPNMIYDIDDMVFLGHSSKANKFIKILKGTRKITFLMKKAKHIVVSTPSNEE
                     FARKYNNAITDISVAINTDVYLPANKYSNNDVITIGWSGSHSTSRYLFLLEEVLRKFA
                     GRSNIRILVIGDTTFKFNGFDCETIPWQSATEVKDLQKIDIGLYPLPDEQWVYGKSGG
                     KALQYMALGIPTIATGIGTNFRIIDDGESGFLVRTDEQWIEKIEYLINHPEERKRIGM
                     NGRMKVEKEYSLHVNKKYYLEIFEKVVG"
     gene            complement(6723..7940)
                     /locus_tag="PKU82_08550"
     CDS             complement(6723..7940)
                     /locus_tag="PKU82_08550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ82961.1"
                     /translation="MNVLYISYDGMTDNLGQSQVIPYLKGLTGYGHHFDIISCEKPDK
                     FKAKEIEIRKLLDSLNIGWYPLPYTKRPPVLSTVIDVVRMYRKAYSLHKENKYDAVHC
                     RSYLSAMIGQDMKKKFGTKFIFDMRGFWPDERIDGDLWNIKNPVYRIIYNYFKKKELQ
                     FFNESDLNVSLTHAGLNEIKKMNVSNEQINKFSIIPCCSDYELFKPLPRDLALVKQLG
                     FEASNKILCYLGSIGTWYMFDEMMAMFKQLHFQDNSFRLFIMTPEPPSMVFDSAKKLG
                     INTDLIKVVSATRQQVPKYLSIAHLGISFIKPCFSKLSSSPTKMGEMLAAGVPLICNS
                     GVGDVERIINETKTGVIIHGFTEDEMQSVAKRAIEMIQVPRENIREQSRPVFALESGV
                     KLYAQAYEKMQKQ"
     gene            complement(8005..>9734)
                     /locus_tag="PKU82_08555"
     CDS             complement(8005..>9734)
                     /locus_tag="PKU82_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOZ82962.1"
                     /translation="FLSGNLNQRKSKGTGLTERQTLGSTPNNFISQSNENSSNGYFGF
                     IRSGADIFLDNRNTITVSGSYMNGYFKPLEFLQTKTDTLFPGYTSSSLSDRNSSSKRE
                     FKNTGGTVAYKRLFPQEGKELTADINFNSVKSDMGGNYSTQNYLGTGEAYGNPILQKQ
                     SIEGENTFVTAQTDMDFPLDKKSKIETGLRGAMRDYSSVNQNFIFDDSQQQYILIPNQ
                     TANYQYVDQVYAAYATYSKSFEKLSLQAGLRAESSFYEGELTQTGQKFSTNYPVSLFP
                     SASASYKLSSKTDLQINYSRRINRPNFFQIIPYSDYSDSLNISKGNPGLKPEFTHSVE
                     LTAMQTMSRKNTLMATVYYKQTDGVITRYLSYETDTILGRNVLVSTFRNANTSYAYGL
                     ELTAQNNIKGWLDITSNINAYQSVINGKNIESNLKVEQFSWFAKLNLNVKLPKNFSVS
                     ISGDYQSKTALQLNSNSGGRGPMGGGTQSTAQGYIRPNYGVDAGLRYDFLKDKAASLT
                     LNVSDIFKTRVNDVHSESDFYIQDSWRKRDAQIFRLNFSYRFGKFDTSLFKRKNQKIN
                     TEGMQDMSM"
BASE COUNT         2992 a         1921 c         1694 g         3127 t
ORIGIN      
        1 acgaccaatc ttctgacctg atgcattaca aatttcgtaa tcgtgtcttg gaattccacg
       61 atcaatcatt tcaaagccta ccaatttgtt tgctatcccc tgttctttct gttgttttaa
      121 tgcatcagag tttgtaaaat tctttgtgaa tttagtaatc cagcctaagc ctgcttctaa
      181 tggtgatgtg ttgtcatcaa tgtcatttcc ataaaggcaa aatcccattt caagtcgcaa
      241 cgtatcacgc gaaccaagac ctgcaggttt tatgtcgaat ggttgacccg cctgcataac
      301 gtcattccag attttttctg catactgatt ttcgaaatac aattcaaaac cacctgcacc
      361 ggtgtagcct gtattggaga taagcacatt ctgacaacct gcaaactctc ctttggcaaa
      421 attgtaatat ggaatgtcgg ccaatgctat ggatgttagt ttctgtaatg tttccattgc
      481 cttaggcccc tgcacggcaa gtaaagaagt tttatcggag atgtcgtgca tttcgacttt
      541 tttgttgtta tgcttttgaa gccaattcca gtctttttca atattcgatg cattaacaac
      601 taacatgaat gttttttcat caatacaata cactattaaa tcgtcaatta ttccaccagt
      661 ttcattgggg aaatagctgt actgtgcttt gccggctgac aatttggaag catcattgct
      721 tgtcacacgc tgaagaagat caagtgcatc atcacctttt aaaataaact ctcccatgtg
      781 agagacatca aacatgccaa ctgcattacg aacagtttca tgctcgtcat taatccccgt
      841 atattgtaca ggcatcaggt agcctgcaaa gggcaccatt ttagcgcctg ccttttgatg
      901 tatagctgtt aaagctgtgt gttttaaggt ggtattctct tccatactat tttaaaatgt
      961 gcggcaaatc tacatgtttt actgcttgtt caatatgatt catgttattt caatatcagg
     1021 tttttatctt tacaccaatt tccacaacgg ttttatatac agatgaaact actaacttca
     1081 gaacaattcc gtatggccga tgcctacaca ataaaaactg agcctataag ttctgttgat
     1141 ttaatggaga gagcagctat ttgttgcagt gatgaaatag caaagtttac agatgaaaaa
     1201 gaccatattt gtattctctg cggacccgga aacaatggag gtgatggact tgccattgcc
     1261 agaatattgg ccgatgcagg aaaaaatgtt tctgttttac ttacagacaa tagcaacaag
     1321 ctaagtcccg atgcagaagc aaattttctg cgcttgcaga aaacttctgt taaaatattt
     1381 tacctcactg atgcaaacct accttttaaa aatacaactt tttgggttga tgcaattttt
     1441 gggacaggat taaataaggc agtagaaaaa aatattgctg atttaattca ccaagtgaat
     1501 tcaaccagaa ctcctgtaat ttccattgat ttaccctcgg gtttatttgc cgacaaacat
     1561 acgccaacga atgttgctgt tattcatgca tcaacaacat taacatttca gcgacttaaa
     1621 cgaagtatgc tttatgcaga aaacgcccac aggattggcg aagttaaagt tcttgatatt
     1681 ggacttgatg aacattatat ggaatcatta cctacaactc aagaactggt tgacaggagt
     1741 ttgataaata aaatactcaa gccgagaaaa cgattttcac ataaaggcaa ttttggtcat
     1801 gctttattga ttggcggaag ctatggaaaa gcgggtgcaa taattctatc agcaaaagca
     1861 tgtgttaaaa gtggtgcagg gttgacaaca gttcataccg taaaaacaac aggagcgaca
     1921 ctacaatctg caattccgga agcaatgttg agcacttgtg aaggtgacaa ggtatgtgac
     1981 ttaccggatt tagcacctta caatgcaatt gctgcaggcc ccggcttggg tattaacgaa
     2041 caaacctcaa gcatgttgaa actacttctt caaaaagcaa aaaaaacatt ggtacttgat
     2101 gctgatgcat taaatataat ttcacaaaat aaaaatctga tagacaatct tccggaaaac
     2161 accattctta ctcctcataa aaaagaattt gaaagactat tcggaacatt tgagaatgat
     2221 tttcaaagaa atgattttca aattgctgct tcagtaaaac acaaaatcat tattgtctta
     2281 aaaggtgctt acacttctat ttctactcct gagggaaaat gtttcttcaa tacaacaggc
     2341 aatgcagggc tggcaaaagg cggcagtggc gacacattaa ccggcattat tactgcattg
     2401 cttgcacaag gcttaaacgc aaccgatgct gcaattgcag cagtttatat tcatggtctt
     2461 gcagcagatt gtgctgttaa aaaaattcac ccttactctt taaccgcaag tgatgtaaca
     2521 gaatttcttt ctgaagcttt tatacagacg gtgtaattac agcaggctca atccagtcgc
     2581 cattatcgcg aatcaattca atgagtgcat caacagcaag tgcggcatca atatttctgc
     2641 ggacgacttc tttgccttta tagagtgtta tttttcctgc accggaacct acatagccat
     2701 aatctgcatc ggccatttca ccgggaccat ttacaataca tcccataata cctatcttaa
     2761 tgcctttgag atgatgtgtt cgtgcacgga ttttagcagt ggtttcctgc aggtcgaata
     2821 atgttcgtcc gcatgaaggg caactgatat attctgtttt tgaaatgcgt gttcgtgcag
     2881 cttgtaaaat tccaaatgct gtttgattaa tatctgccgg actaactaat tctgaacaaa
     2941 gccaaacacc atcacccata ccatccaata acaatgcacc tgcatcagtg gaacaatgta
     3001 aacgaaatgt ttctaaatca gctttagcat aatcacgctt tatgatgaca ggtaaagtat
     3061 ttttctgctg catcatatca acaaacacat tacgtaaatc cggcattgca tgatcgttag
     3121 atgtttttaa gacaagaaca gcatcagaaa gtttatcaac ttgcaactgt tgtttaaaaa
     3181 aatcttcagt gtttaattca ataaacttta tggcggcagt gcattttaat gcatcattca
     3241 gctcattcac attaaacaac ggataagttc tttcatcatc ggaatgttgt agccacattt
     3301 tattgttgta aatgacaccc aatgttccgg gcaatgcaaa tgaaatatgt tgattaccta
     3361 cataaataaa atcacatgcc tgatcgctga tatgccattt atccaatgac tgtgaataat
     3421 tatatccaac aggaaataat gaagcaggag aaatattttg cttgcctgaa aaatctgcaa
     3481 tcacaaccgg gacatgattt cctccaacat tcaaaacagc ttttgttagt cgtttgctgt
     3541 aactgaatgg agaataaggt agtttatgat ggttaatagg ttttattgtt tggtgtgatg
     3601 tgcgattatt ataacgtgac accaattttt gtgcaacagg aatttcaagt tccggatctt
     3661 cggttaacga aactcttatg gtatcgccaa taccatcttc gagtaatgtg cctatacctg
     3721 atgctgattt tattcgtccg tcttcagcct cgcctgcctc tgttacacca agatgcaaag
     3781 gaaaaagagc aggcaaagtg ttttgcatat tattcaaatc cataaactct gcacgcattt
     3841 tagcaatcaa aagtctatat gcctgaacca ttacctgagg attacttgct ttcatagaaa
     3901 gtacgatgct ataataacct tcatccatac aaattcgcaa aaactcaaga gcagactcca
     3961 ccattcccaa aggtgtatcg ccataacgac tcataatacg atcgctcaaa gaaccatgat
     4021 tggtaccaat gcgcattgca gtaccatact ctttacaaat ctttactaat ggaataaacc
     4081 gttcgcggat tcgctctaac tcttcagcat aagttacatc agtatattca atagtgtcga
     4141 actttttctt gtcagcatag ttgccgggat tcacccttac tttctccaca atacgagctg
     4201 cagtttctgc ggcattaggt gtaaaatgga tatcggcaat cagaggtgtt ttatagcctc
     4261 tcttgcgaag ttcgtcttta atattctgaa gattacgtgc ctcattgatg cttggtgcag
     4321 tgattcgcac aaattcacaa cctgccacaa tcatcctgat gctttgttca actgtagcct
     4381 tggtgtcaag agtgtctgta gtggtcatac tttgaacacg aatgggatga ttagctccca
     4441 ttgctacatc gccaatcttt acctcaagtg tttttagtct ttggtaagaa gtaaggctgt
     4501 tacaatatag ttggaaaata ttttgatctg tgagggcgct atccattttt aaaataaata
     4561 tttagtatgc aaatatcgga attgtatttc tttagattag cataaccgag taaagacatt
     4621 aaagttttac cacccgtttt tgaagcacct ttttgtcaga ttccagcagc agataataag
     4681 tgccgggtgc aagattttta ggataaattg taagttgatc aagagcctgc ctttgcttgc
     4741 tgtgataaac gaccttacca acaatattca ctaatttaat atcgtacttt ctgtataaaa
     4801 aatcttcaga gtgaatgcat atctgatctg tgaaaggatt cggataaagg cttacatagt
     4861 cttttttgat tacttcactt tcaccaacaa caactacaga atcgcagtca accaaggtgt
     4921 tgggctgata caaacctaat gttgaatcgt tgtaacatct aaaagcagtt atctgaggat
     4981 cgcataaatt gaattgtgta agtaaaaaat tcaaactgcc aataccttca ataataggcc
     5041 cttcaaaaac aaatggaatg tgagatgaat cggcataaga aatgtaaaat atttttcggg
     5101 gatgattgtt gatattaacc atgttcactg agtcaacacg aatacgcaca gtgtctgtaa
     5161 gtggtgccgg tggcggttga ttaatggtgt ttgatactat gttccatata tcacccggct
     5221 gtgcattaaa atcgtaaagc atggtaaagg tgttgagatt gtagagaaaa cgataaacag
     5281 tatcgttact ctgataaaca ggcaaataat taaagccaac acaagtaaaa ggagcaatct
     5341 gtaggttagt gcatgtaatg ccattaatga cagtagttcc agttgcattt acttcaacag
     5401 ggccgcagga ataaggaaat gtaactgtac attgcgtgta gtgccactgt gcaccaacag
     5461 gtgcaaactg tgctttagct acaaaatgac tacaagctat aagcaataca aaaattatct
     5521 ttttcattgc agtgctaaga tacttaaaac aaagtgacaa acaaatattc aaccaactac
     5581 cttttcaaat atttcgagat agtatttttt gtttacatga agtgaatact ctttctcaac
     5641 cttcattctt ccattcatgc caattcgttt tctctcttca gggtgattta tcagatattc
     5701 aattttttca atccactgtt catctgttct taccagaaac cctgactcgc catcatcaat
     5761 tattctgaag tttgtcccga ttccggttgc aatggttgga attcccaaag ccatatattg
     5821 caatgccttg cctccactct tcccataaac ccattgttca tcaggaagtg gatataggcc
     5881 aatatcaatt ttctgaaggt ctttaacctc tgttgccgat tgccagggaa tggtttcaca
     5941 atcgaagcca ttaaacttaa acgttgtgtc tcctattacc aggattctga tattactgcg
     6001 accggcaaat ttcctcaaca cttcttccaa caaaaataaa taacggcttg tactgtgact
     6061 gccactccat cctattgtta taacatcatt gttgctgtat ttatttgcag gaagataaac
     6121 atctgtgttg atagctactg aaatatcagt aattgcattg ttatactttc tggcaaactc
     6181 ctcattagaa ggtgttgaaa caacaatgtg cttagccttc ttcatcagga aagtaatttt
     6241 tcttgtacct tttaatattt taataaactt attggcttta gaagaatgac caagaaaaac
     6301 catgtcatca atatcataaa tcatattagg gtttagcagc acaaaaagcc attcaaacag
     6361 cggcaatccg aatggtgtta cccacaaaaa aatatacact gcatcataac gccataacgt
     6421 aaacagttgc aaaaagcgtt ttatgtagcc aaacaaagtc cagaatattt tctctggaac
     6481 atttccgggt ttataaacaa tgctgttaaa acgataggtc atgaaagatg aaacatgtat
     6541 atcatatcct tcttttctga acaaattcag atactgctca taacgcaaac gttgccccgg
     6601 tgcagtatct tcgggataag ggcaaatcag tagaattttc ttcttattgt ggctcataaa
     6661 tttttgttgc ctgcaaaaac aaggtcagca aattgtctgc gaaaataaag atgattttgt
     6721 aattattgtt tttgcatttt ttcataagcc tgtgcatata gtttaactcc gctttcaagt
     6781 gcaaaaaccg gacgagattg ttctctgata ttctctcttg gaacctgaat catttcaata
     6841 gctctttttg ctacagactg catttcatct tctgtaaaac catgaattat tacacctgtc
     6901 tttgtttcat taatgatacg ctcaacatcg ccaacaccac tgttgcaaat caatggaact
     6961 cctgcagcaa gcatctcccc catctttgtt ggcgaactgc ttagttttga aaagcaaggt
     7021 ttgataaatg aaattcccag atgcgcaatt gataaatatt ttggcacctg ctgtcgcgtt
     7081 gcactcacta ccttgattaa atctgtattt attcccaatt tttttgccga atcgaaaacc
     7141 attgatggag gttcaggtgt cataataaac aggcggaagg aattatcctg aaaatgcaac
     7201 tgcttaaaca tagccatcat ctcatcaaac atataccatg ttcctattga accgaggtaa
     7261 cacaatatct tgtttgatgc ttcaaaacct agctgcttaa ccaatgccaa atcacgcggt
     7321 aaaggtttga acaattcata atctgagcaa catggaataa tagagaactt gtttatttgc
     7381 tcgttgctga cattcatttt tttaatttcg ttcaaccctg catgtgtgag actgacattc
     7441 aagtctgact cattaaaaaa ctgcagttct tttttcttga aataattata aatgattctg
     7501 taaactggat ttttaatatt ccacaaatca ccgtcaatgc gttcatcggg ccagaagcct
     7561 cgcatgtcaa aaataaattt ggtgccaaac ttctttttca tgtcctgacc aatcatagca
     7621 gagagatagc ttcgacaatg cactgcatca tacttgtttt ctttgtgcag actgtatgct
     7681 tttctgtaca tacgaaccac atcaattact gttgataata cagggggtct ttttgtatat
     7741 ggcaaaggat accatcctat gtttaaagaa tcaagcaact ttctaatttc tatttcttta
     7801 gctttaaact tatcgggctt ttcgcaactg ataatatcaa aatgatgacc ataacctgtg
     7861 agtcctttca gatagggaat tacctgcgac tgaccaagat tatctgtcat tccatcataa
     7921 gaaatataaa gtacattcat aaagcgtcca aaaatattgc atttgaaccg aattcagatg
     7981 cattaacgaa cgaaagaata aacactacat gctcatatcc tgcatacctt cagtatttat
     8041 tttctgattt ttcctcttga atagtgaggt atcgaatttc ccaaagcgat agctgaagtt
     8101 taatctgaaa atttgtgcgt ctcgtttgcg ccagctatcc tgtatataaa aatcagattc
     8161 tgaatgcaca tcatttactc ttgtcttgaa aatatcagaa acatttagcg taagtgatgc
     8221 tgctttatct ttaagaaaat cataacgtaa accagcatca acaccataat ttggtcttat
     8281 ataaccctgt gccgtacttt gagtgccgcc acccattggt ccacgtcctc cactattact
     8341 atttaactgt aatgctgttt tggattgata atcacctgaa atactaacag aaaaattctt
     8401 aggcagttta acattaaggt tgagttttgc aaaccaacta aactgttcaa cttttagatt
     8461 tgactcaatg ttttttccat taatcacact ctgataagca ttaatatttg aagtaatgtc
     8521 gagccaacct ttaatattat tttgtgctgt taattctaac ccataagcat agcttgtgtt
     8581 tgcatttcgg aaggtgctta ccaatacatt acgtcccaaa atagtatccg tttcatagct
     8641 cagataacgg gtaattaccc catcagtttg tttataataa actgttgcca tcagtgtatt
     8701 tttacgactc atagtttgca tggctgtcaa ttctactgaa tgtgtaaact caggtttcaa
     8761 tccggggttt cctttactga tgtttaaaga atcggaataa tcagaatatg gaattatctg
     8821 gaaaaagtta ggtctgttta ttctgcgtga ataatttatc tgcaaatcag ttttactact
     8881 cagtttataa cttgccgatg cacttgggaa caaactaaca ggataatttg tgctaaactt
     8941 ctgtcctgtt tgtgtaagtt caccttcata gaacgagctt tctgccctta gtcctgcctg
     9001 taatgataat ttttcgaatg acttactgta ggttgcataa gctgcataaa cctgatctac
     9061 atattgataa tttgctgtct gattaggaat taaaatatat tgttgttgcg agtcgtcaaa
     9121 aataaaattc tgatttacac tgctgtaatc gcgcatagca ccacgcagtc ctgtttcaat
     9181 tttcgacttt ttatcaagcg ggaaatccat atcggtttgt gctgtaacaa atgtgttttc
     9241 accttcaata ctttgcttct gcaaaatagg attgccgtaa gcttcgccag tgcccagata
     9301 attttgtgta ctgtagtttc ctcccatatc actctttaca ctgttgaaat ttatatctgc
     9361 agtaagttct ttaccttctt gcgggaaaag acgtttatag gcaactgtgc cacctgtatt
     9421 tttaaattca cgtttgcttg aagaatttct gtctgacaaa gaagaagatg tatatcccgg
     9481 aaataatgta tccgtctttg tttgtaaaaa ctccaatggt ttaaaatatc cattcatata
     9541 acttcccgaa acagttatgg tatttctgtt atctaaaaaa atatctgctc ctgaacgaat
     9601 aaatccaaaa tagccattgc tggaattttc attactttgg cttataaaat tatttggagt
     9661 ggagcccagt gtttgtcttt ctgttagtcc agttcctttt gatttacgct gattgagatt
     9721 gccgcttaaa aaca
//
LOCUS       DAOOOL010000099         9665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_653_length_9665_cov_83.160978, whole genome shotgun sequence.
ACCESSION   DAOOOL010000099 DAOOOL010000000
VERSION     DAOOOL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_9665_cov_83.160978"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1296
                     /gene="recA"
                     /locus_tag="PKU82_08560"
     CDS             283..1296
                     /gene="recA"
                     /locus_tag="PKU82_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOZ82963.1"
                     /translation="MSTVQAPDKEKLKVLQAAIERIDKTYGKGTIMRMGEDAIEPVEA
                     IQTGSISLDIALGINGLPKGRVIEIYGPESSGKTTLAIHAIASVQKNGGIAAYIDAEH
                     AFDRFYAQKLGCDIENLLISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPKSEI
                     EGEMGDSKMGLQARLMSQALRKLTASISRTKCCCIFINQLREKIGVMFGNPETTTGGN
                     ALKYYASVRLDIRRIGQIKDGDSVAGNRTRVKVVKNKVAPPFRQAEFDIMYGEGISKT
                     GELVDLGVELNIVKKSGSWFSYGDTKLGQGRDAVKQLFSDNPELADEIENKIKEAMNL
                     KTA"
     gene            1423..2418
                     /locus_tag="PKU82_08565"
     CDS             1423..2418
                     /locus_tag="PKU82_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ82964.1"
                     /translation="MTLKKHYILYFSVLIFTACTNGKKETEEPKDKIAQLTESIKADS
                     TNPKNYFDRATAYSQKSMFDLAYFDMEKAMKIKPSDPEYLMLYSDLSFRMNKVKDARD
                     ALVKVREMQPSNYDAAFKLAEIFLYTNNQDQSLHYLDTVLQKDPKNTKALLMQGFNLK
                     EKKDTAAAITAFRNAVAIDPKFYEAQIQLGLLMYLQNNKLCVDYYTNALNIKANSEEA
                     MYGLALWYQDHQEFNKAIQFYTDIIQINPKNKNAHFNLGYIHHEYLKVYDQAIKHYSD
                     AIAADPNYAEAYYNRGLCYEYVGNITAAKDDYSKALQIRKGNYPHAQEGLQRVSR"
     gene            complement(2449..4164)
                     /locus_tag="PKU82_08570"
     CDS             complement(2449..4164)
                     /locus_tag="PKU82_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82965.1"
                     /translation="MKKTIKLLLIFLMLFTSVAAQTPSWHWLARVGGYSGNGNGAGGP
                     DEWVKDVKTDAQGNVYVCGRVCYGANFNGDTMTTYQGYFSLFLAKLNCAGDLVWVKTA
                     GNTSNLYNTEAYGLALDNYGNIYLTGYLGAHPLSPCQFFDTLITEETYDMFLAKLDTA
                     GNFKWVKLAGPGPGTLGTGGRQIQITDDDMINVIGSGGSGVIFPGYNYFSLSWCFTAR
                     FDTSGNINRFQNLGSVYGPFYRDCKISPTGDQYITGYFVADSVVIGDSTLYLTNAPNV
                     GIDLFLAKFDSSGNFKWVKQIGTPSANGMKGYGVDIIGSDIVLTGMVHPGTIIGSTTI
                     TNPLYLLNSVVPFVARLYNQDSVVWVSSIQCSSAYGMPTGGVSVYNNQIATAGMFLGK
                     AVIGSDTITAVSYRDIFLSIMDASTGNFTKGVNLPGTGSSEEPQCITHDSSGNIYVGG
                     SYDGTLTVNGINYPYQGGNSDGFIAKWGSNCTVGLEEQVPLPAENLFLYPNPATSEIK
                     IQNKEQLIVAIEIYDMVGKKIMYRKVSGKESTIQIDVSSFSPGMYVVKTKGKTNMLTA
                     KFVKE"
     gene            complement(4173..9632)
                     /locus_tag="PKU82_08575"
     CDS             complement(4173..9632)
                     /locus_tag="PKU82_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ82966.1"
                     /translation="MLFLLLPGFLQAQYAVVGNSEDCMNNISVYSVSGTPLPATYIWN
                     VTGGNVVSTGTGTVSIDWSSVGSGSVGVTLYDVSGAVIGTPSLTVTVNPLPQPVLTTD
                     FTNNCVIYKEHEGQVIPTVVNDTCWHVCENGIVHYFANPVNVGSSFNWNVTGAQSFVA
                     ISNNEIEVIWGSAGNGTVSVTETDGNGCVNTVEKCIVIIESPQAIITAFPGDVDCRLG
                     GVITVCLEQNALFFGNANLGNSGSPIVSWLWDFGDGTVSTHRDEVHSWQTPGIYQTTL
                     TVTNACGCTGICYVSVEVDPVNGVNIECPTPVCQGDTGKYYTDAVNCSAYNWVVTGGN
                     ILSPMPYGSTIQVQWTGGNGNGILCLDNTNGTCGNYCPSETCVEIPIISSTSSIDGPS
                     LVCIGSQTKYNVPAMTGCVYNWQITGSSGTIVSGQGTNEIMVDWTSAGTSTISVNYNN
                     ALLNCGGTANLTVETKQPFSINGPTKGCPGVDVVLTANNPVNVLLDWTITDASQNIIY
                     VGSGTGVPDFTITGWSYPSGTYIISALDNSNSYCNYTASITIEIKDPPPSPAMPVGDN
                     PICPGTVHLYSGTTMNAGYYLEWSVTGGTPTIGTGNELSIGWINTTPPHNYSISLVQV
                     ESSTGCKSLPTVLPIQERLYINPVITGNFTPCINKQVTYQISNAVPNGNYIYDQLEWV
                     IVPATAGSVISGQGTTTVTVQWNNQPSPVNVYDTYLQVKTSLCGSPPFPYPNPLFDPP
                     NISLGGAPALAITAIPNPACENTPITFTATPALPGNYTWSFGDGSSGNSPLNAVHTYS
                     LTQGASLTTFDVTLTVDDGGCQSYASTKIDVNPAPLYSLTLSATTFCPPAVTNITASL
                     VQQSGSFNYSWSNNASNNSNVNTLNYLDAQPFDVTITNTITGCARTESFSISTCSPNP
                     PLCNPTPLLYIGFTTSPASDCFTVNFVGSINPTPASYLWDFGDNTSDNTTLTPSHTYS
                     TAGQFSACLYAFDNTGLQYCDNYCLPVSVPLFPNFITSLHCSSTPGSYDVEFIDLSSF
                     TIPITTWQWFSGTTQFSTSNHPTITLAAGTYNIKLKIGNGTDFCERTLTINIPAPPSP
                     AFTVAGGPFCEGQTVVQFSASNTNVHHALWDFGDQATYNPQNPAATINAQRVFESGLS
                     IYSVNLTVTDNYGCTYSSTQQVTVNDNNLGGSISPVLISFCPGGNGSLALDYITIPGG
                     PYTQPTSWLWTGGVTTPTLTVNQTGQYFVTVSDNNGCSYSPASPGQAAVVNVPKPFIW
                     GDLTLCEGDNINLDGNQGSAVQYNWTIVDPNNVTNTFSQSSIHIAGAVAGTYHVTLDI
                     TANGCTRSTSVDVLVNPLPPLPIIQSNVPQACEGQPIDLTVINAGSYWVNWSNGASGP
                     VITVYTAGAYQATLTDANGCTNHATFEVHENPFSGFMMTGCVQYCDTVPVTVFGSGSL
                     SFNKWIWYVDGVAQASGQQSPVQDLNMGLLSPGVYVVTLQLVVGYPDGTVCEMMSDAL
                     EITIIPCPCRLYPQGKIYCMNEVNGDVQLLNNYHFEIVTNYGCSANPNVMVSSPDGGV
                     SMLPNSNPPQLLTGILSTYSAYPDNVCFDINITDSNKPMCSCNYRYCMKLPKCGVPTG
                     CSTEVKIDNVQCIGHDANGNRIYSFTLTLLNSPQLFSMFTAQGGVLGAMPATVGPGTT
                     IINSSITVNNNASHFCITLHGYNFTTYTACKTTGCSGNLPPCGIPPSRNGEKDTTTPL
                     RVSYQNDANDAVQNTSLSLMPNPAKNKVTLHYSTRSGGIITIASAVGQIIYNENVATA
                     GMSELSTQQWLPGIYFVTLQSNAGDCLVQKLVIIK"
BASE COUNT         2953 a         1962 c         1943 g         2807 t
ORIGIN      
        1 gaacaaatat catctacatc ataaaattta ttaaaattca gctaataagc tgaatacatg
       61 ccacttacgt aaattcaccc aaaaccttaa aacgtgctgc ttgattgcat tacgaccatt
      121 aaagttgttg attttaaatt aaaattcagg gctaattgac agatttacaa tcacaacgag
      181 gacggcattg ccgctgttga aaacaaaata ttgaattttc ataaatcaga attggctaaa
      241 accattactt tgtcgctata aacattcaaa aaaataagac acatgagcac agtacaagca
      301 cccgacaaag agaaattaaa agtattacaa gcagccattg aacgcattga caaaacctat
      361 ggtaaaggca ccatcatgcg catgggcgaa gatgccattg aacctgttga agccattcag
      421 acaggaagta tttcattaga tattgcatta ggcattaacg gtcttcctaa aggacgtgta
      481 attgaaatat acggacccga atcttcaggt aaaacaacat tagccattca cgctattgcc
      541 agcgtacaga aaaatggtgg cattgctgcc tatatagatg cagaacatgc attcgatcgt
      601 ttttatgcac agaaattagg ttgtgatatt gagaaccttc ttatatcgca gcccgacaat
      661 ggcgagcagg cacttgaaat tgcagacaat cttattcgct caggtgcaat agacattatt
      721 gttattgact ctgttgctgc tttaacgcca aagagtgaaa ttgaaggcga aatgggcgat
      781 tctaaaatgg gattgcaggc acgtttgatg tcgcaggcat tgcgtaaact tactgcaagc
      841 atcagccgaa ctaaatgttg ctgtattttt atcaatcagc ttcgcgaaaa aattggtgtt
      901 atgttcggaa atcccgaaac aacaaccggt ggtaatgcat tgaaatatta tgcatcagtg
      961 cgtttagaca ttcgcagaat aggacagata aaagatggtg acagtgttgc aggaaaccgc
     1021 acccgtgtta aagtggtgaa aaacaaagta gctcctccat tccgtcaggc agaatttgac
     1081 attatgtatg gcgaaggtat ttctaaaaca ggagagcttg ttgaccttgg tgttgaatta
     1141 aacattgtaa agaaaagcgg ttcgtggttc agctatggcg ataccaaact tggtcaggga
     1201 cgcgatgctg tgaaacaact ttttagtgac aatcctgaac ttgccgatga gattgaaaat
     1261 aaaattaaag aagcaatgaa tttaaaaact gcttagaaaa ataattattt gtttattggt
     1321 aaaagaaaag agctgccggt tttcggtggc ttttttcatt tcataccaat tgtatatgat
     1381 ggattgtgtt taaaccgata tttgcaccac aataacatta gaatgacatt gaagaaacat
     1441 tacatacttt atttttcagt gctgattttt actgcctgca caaatggaaa aaaagaaaca
     1501 gaggagccaa aagataaaat tgctcagctt acagaaagca tcaaagccga cagcaccaat
     1561 ccaaaaaact atttcgaccg tgccactgcc tattcgcaaa agagcatgtt tgatttggct
     1621 tattttgata tggaaaaagc aatgaaaata aaaccatctg acccggaata tctgatgctt
     1681 tattcagacc ttagtttcag aatgaataag gtaaaagatg cacgcgatgc attagtaaaa
     1741 gtgcgcgaaa tgcaaccatc aaactatgat gctgcattta aattggcaga aatattttta
     1801 tataccaaca atcaggatca gtcgttgcat tatttagata cagttttaca gaaagaccct
     1861 aaaaacacca aggcattgct gatgcagggc ttcaacttaa aagagaaaaa agatacagcc
     1921 gctgccatta cagctttcag aaatgctgtt gccattgacc ctaagtttta tgaggcacaa
     1981 atacaattgg gtttactgat gtatttacaa aacaacaaac tgtgcgttga ctactacacc
     2041 aatgcattaa acattaaagc caacagcgaa gaagccatgt atggtctggc attgtggtat
     2101 caggatcatc aggagtttaa taaagccata cagttttaca ccgacattat tcagataaat
     2161 cctaaaaaca aaaatgcaca tttcaacctt ggatacatac atcatgaata tctgaaagtt
     2221 tacgatcagg caatcaaaca ttattccgat gccattgcag cagaccccaa ttatgcagaa
     2281 gcctactata accgtggttt atgttatgag tatgttggaa atattacagc agcaaaagac
     2341 gattacagca aagcactgca aataagaaaa ggtaattatc cacatgcaca ggaaggactg
     2401 caaagagtgt cgaggtagag gagatgaatt tttgagacac attctaccct actccttcac
     2461 aaactttgct gttagcatat ttgttttgcc cttggttttt actacataca tgcccggtga
     2521 aaatgacgaa acatctatct gaatggtaga ctctttacca gaaacttttc tgtacattat
     2581 ttttttacca accatatcat aaatttctat agcaacaata agttgctctt tattttgaat
     2641 ttttatttct gatgttgcgg ggtttggata taaaaataaa ttttctgcag gcaatggcac
     2701 ttgttcttct aaacctaccg tacagttgct accccactta gcaataaaac catcggagtt
     2761 gccgccttgg tagggatagt ttattccatt tacggttaaa gtgccatcgt aactaccacc
     2821 aacataaata ttgccgctgc tatcgtgagt tatacactgt ggctcttcac tactgcctgt
     2881 acccggtaaa tttacacctt ttgtgaaatt gcccgttgag gcgtccatta ttgataaaaa
     2941 tatatctcta taacttactg ctgtgatagt atcgctccct ataactgctt ttcctaaaaa
     3001 cattcccgca gttgctattt gattgttata tacagaaaca ccacctgtgg gcatgccata
     3061 agcagatgaa cactgaatgg acgaaaccca cactacacta tcttgattat ataacctagc
     3121 tacaaaaggc actacagaat ttaaaaggta taatgggttt gtaattgtag ttgatcctat
     3181 aattgtgcca gggtgtacca tgcctgttaa aacaatatca gagccaataa tatctactcc
     3241 gtagcctttc attccattag ctgatggagt acctatttgt ttcacccatt taaagttacc
     3301 tgaggaatcg aatttagcca aaaacaaatc tattccaaca tttggtgcat tagttaagta
     3361 caaagtagaa tcaccaatta caacagaatc tgcaacaaaa tagcctgtaa tgtactgatc
     3421 gcccgtggga cttattttgc aatctctata aaatggacca tatacactgc ctaaattctg
     3481 gaatctgtta atatttcctg aagtatcaaa acgcgcagta aaacaccaac taaggctaaa
     3541 ataattatag ccgggaaaaa taactcccga tcctcctgaa ccgataacat taatcatatc
     3601 gtcatcagta atttgaattt gtcttccgcc tgtacccaaa gttcccggtc ccggacctgc
     3661 taatttaacc catttaaaat ttcctgccgt atctaacttt gcaagaaaca tatcgtatgt
     3721 ttcttctgtt ataagtgtat caaaaaactg gcaaggactt aatggatgcg caccaaggta
     3781 gcctgttaaa taaatattgc cataattatc taaagcaagg ccataagctt ctgtattata
     3841 caaattgctt gtgttacctg cagtttttac ccaaactaag tctccggcac agtttaattt
     3901 agccagaaac aagctaaaat aaccctgata ggttgtcatg gtgtcgccat taaagttagc
     3961 cccatagcat accctgccgc agacatatac attgccctgt gcatcggtct ttacatcttt
     4021 tacccattcg tcaggtccac cggcaccatt gccattacca ctgtagccgc ctacacgtgc
     4081 cagccaatgc caacttggtg tttgtgctgc cacacttgta aacagcatca aaaatatcag
     4141 caataatttt attgtctttt tcattgtttg tgctatttga tgatgactaa tttttgaacc
     4201 aaacaatcgc ctgcattgct ttgcaaggta acaaaataaa taccgggcag ccattgctgt
     4261 gttgaaagtt ctgacatgcc tgctgttgcc acattttcgt tatatattat ctgcccaaca
     4321 gcagaagcta tggtgataat gcctcccgaa cgggtgctgt aatgtagtgt aactttgttt
     4381 ttagccggat taggcatcag gctaagcgat gtattctgaa cagcatcgtt agcatcattt
     4441 tgatagctta ccctcagtgg tgtagttgtg tctttttctc cgtttcttga tggaggaata
     4501 ccacatgggg gcaaattacc cgagcaacca gtagttttac aagcagtgta ggtagtaaaa
     4561 ttgtagccat gcaaggtgat gcagaagtgg cttgcattat tgttgactgt tatagaactg
     4621 ttgatgatgg ttgtgcccgg tccaactgtt gcaggcatag cgcctaaaac acctccttgt
     4681 gccgtaaaca tggagaatag ctgtggagaa ttaaggagtg tgagtgtaaa actatagatt
     4741 ctgttgccat tggcatcatg accgatacat tgcacattgt ctatcttaac ttctgtgcta
     4801 cagcctgtgg gaactccgca ttttggcaat ttcatacaat agcggtagtt gcagctacac
     4861 atgggcttgt ttgagtctgt tatgttaatg tcaaaacaaa cattgtcagg ataggcactg
     4921 taggttgata atattccggt aagtagttgc ggtggatttg aattcggcag catagatact
     4981 ccgccatctg gcgaagacac cataacatta ggattggcag agcatccata gttggttact
     5041 atctcaaaat gataattatt caataactga acatcaccat tcacttcatt catgcaataa
     5101 attttacctt gcggataaag tctgcaagga catggaatga tggtaatttc aagtgcatct
     5161 gacatcattt cgcacacagt gccatcagga tagccaacta ctaactgaag cgtaactaca
     5221 tatacacccg ggctgagcaa tcccatatta aggtcttgta cggggctttg ctggcctgaa
     5281 gcttgcgcaa caccatcaac ataccatatc catttattaa aagacaaact tccactaccg
     5341 aatactgtta caggcactgt gtcgcaatat tgcacacagc ctgtcatcat aaatcctgaa
     5401 aaggggttct catgcacttc aaaggttgca tgattggtgc agccattggc atcagttagt
     5461 gttgcctgat aggcaccggc agtataaaca gtaataacag ggccgcttgc accattgctc
     5521 cagtttaccc aataactacc ggcattgata acggtaagat caatgggctg cccttcgcag
     5581 gcttgcggca cattgctttg aataataggc aatggcggta atggatttac taaaacatct
     5641 acagaagtgc tgcgggtgca accattggca gtaatgtcaa gtgttacatg atatgttccg
     5701 gcaacagccc ctgcaatatg tatggaagac tggctaaagg tattggtaac attattgggg
     5761 tcaactattg tccagttgta ctgtacagca ctaccctgat tgccatctaa attaatgtta
     5821 tcgccttcgc acagtgtcag atcgccccag ataaaaggct ttggcacatt tacaactgca
     5881 gcctgaccag ggctggcagg actgtagctg caaccgttat tgtcgctcac tgtaacaaaa
     5941 tactgccctg tttgatttac cgttaatgtg ggcgtagtta caccaccagt ccatagccag
     6001 gaggtgggct gtgtgtaagg accacctgga attgtaatat aatccaacgc caaacttcca
     6061 tttcctcccg gacaaaatga tattaataca gggcttatgc tgccacctaa attattatca
     6121 ttaaccgtta cttgttgtgt gcttgagtag gtgcagccgt aattgtcggt aactgtaaga
     6181 tttactgaat aaatgctaag tcctgattca aacacccgct gtgcattaat agtggcagca
     6241 gggttttgag ggttataagt agcctgatca ccaaaatccc aaagggcatg gtgtacgttg
     6301 gtgttgcttg cactaaactg tacaacagtt tgcccttcgc aaaatggccc gcctgcaacg
     6361 gtaaatgcag gtgagggtgg tgcaggaata tttattgtaa gtgttcgttc gcaaaaatct
     6421 gtgccgttgc ctatttttag tttaatatta taggtgcctg ctgccaaagt aatggttggg
     6481 tggttgcttg tgctaaactg tgtggtgcct gaaaaccact gccaagtggt aattgggatg
     6541 gtaaaagatg aaaggtcaat aaattcaacg tcataacttc cgggtgtact gctgcaatgc
     6601 aacgaggtaa taaaattcgg aaaaagcggc accgataccg gtaagcaata attgtcgcaa
     6661 tactgtagtc cggtattatc gaaggcatat aggcaagcac taaattgtcc ggcagtgctg
     6721 tatgtatgtg aaggggtaag tgttgtgtta tcactggtat tatcaccaaa atcccataaa
     6781 taacttgcag gtgtagggtt gatgctgcct acaaaattta ctgtaaaaca atctgatgcc
     6841 ggtgaagtgg taaagccgat ataaagaaga ggggttggat tgcacagagg cggattgggt
     6901 gagcaagttg aaattgaaaa actttctgtt ctggcgcaac cggttatcgt attggtgatg
     6961 gttacatcaa atggttgtgc atctaaataa ttcaatgtat taacgttgct attattagat
     7021 gcattattac tccacgaata attaaaactt cctgattgtt gcaccagcga ggctgtaata
     7081 ttagtaacag ccggtgggca gaacgttgtt gctgaaagtg tcaaagaata taatggagca
     7141 gggttaacat caatttttgt gctggcataa gattggcatc cgccatcatc aacggtcaag
     7201 gtaacatcaa aggttgtgag gctggctccc tgagtcagac tataggtatg cactgcattt
     7261 aaagggctgt ttccgctgct gccatcacca aaactccagg tataattacc gggaagcgca
     7321 ggagtggctg taaaagttat tggggtattt tcacaagcag gatttggtat tgcagttata
     7381 gctaaagcag gtgctccgcc aagtgaaata ttgggtgggt cgaacaatgg attcggataa
     7441 gggaatggcg gtgagccaca gagcgaagtt tttacctgaa gataggtgtc ataaacatta
     7501 accggagaag gttggttatt ccactgcaca gttacagtgg tagtgccttg cccgcttatt
     7561 acacttccgg cagtggcagg aacaattacc cactctaatt gatcgtatat ataattgcca
     7621 tttggcacag cattggaaat ctgatatgtc acctgcttat taatacatgg tgtaaaattt
     7681 cctgttatga caggattaat ataaagtctt tcctgtattg gcaatactgt tggcaatgat
     7741 ttgcatccag tagatgactc tacctgcacc aaactgatgg aatagttatg tggaggtgtt
     7801 gtattaatcc agcctataga taattcattg cctgtgccta ttgttggagt gccaccagtt
     7861 acactccatt ctaaataata tcctgcattc attgttgtgc cgctgtacaa atgtacagtg
     7921 cccggacata ttgggttgtc accaacaggc attgcaggtg acggtggcgg atcttttatt
     7981 tctattgtta tgcttgctgt gtaattacaa taactgtttg aattgtctaa tgctgatata
     8041 atataggtgc cactaggata agaccatcct gtaattgtaa aatccggaac tcctgtacca
     8101 ctgccaacat atatgatgtt ttgagaagca tctgttattg tccaatccaa tagtacattg
     8161 acaggattat tagctgtgag tacaacatct actcccgggc aaccttttgt tggtccatta
     8221 atactaaaag gctgcttggt ttcaacagtt aaattggcgg tgcctccgca attcaataag
     8281 gcattgttat aatttacact tattgttgaa gttccggcac tggtccaatc taccattatt
     8341 tcattggtgc cttgccctga aacaatagtg ccgctgctgc ctgttatctg ccagttataa
     8401 acacaacccg tcatagcagg cacattgtat tttgtctgag agcctataca aaccaatgag
     8461 gggccatcaa tactacttgt tgaagatata ataggaattt caacacatgt ttcacttgga
     8521 cagtagttac cacaggtacc gttagtatta tccagacata aaatgccatt gccattgcca
     8581 cccgtccact gtacctgtat ggtgctgcca taaggcattg gtgaaagaat gtttcctccg
     8641 gtaactaccc aattatatgc actgcaattc actgcatcgg tataatattt acctgtatcg
     8701 ccctggcata caggagttgg acattctata ttcacaccat ttacagggtc aacctctaca
     8761 gatacataac aaatgcctgt acaaccacat gcattggtta cggtgagtgt tgtctgatag
     8821 attccaggcg tttgccacga atgtacttcg tctctatgtg tagaaactgt tccatcgcca
     8881 aaatcccaca accaacttac aattggtgaa cctgaatttc caaggttagc atttccgaaa
     8941 aataatgcat tttgctcaag acaaacagta attacgccac ctaaacgaca atccacatca
     9001 cccggaaatg cagtaattat tgcctgtggc gattcaataa tgacaataca cttttcaacc
     9061 gtatttacac aaccattgcc atcagtttct gttacagaaa cagttccgtt gccggcactt
     9121 ccccatataa cttctatctc attatttgaa atagctacaa aactttgcgc acctgttaca
     9181 ttccaattaa atgatgagcc aacgttaaca ggatttgcga agtaatgtac tatgccgttt
     9241 tcacaaacat gccagcaggt atcatttacc actgttggta ttacctgccc ttcatgctct
     9301 ttatagataa cacagttgtt ggtgaaatct gtagtaagca caggttgtgg tagtggattt
     9361 actgttacag taagtgatgg tgttcctatt actgcacccg aaacatcata caatgttaca
     9421 cctacagaac cagaaccaac agaactccaa tctatactca ctgttcctgt gcctgtacta
     9481 acaacgttgc ctcctgtaac attccagatg tatgttgccg gaagtggtgt tccgctgaca
     9541 gaataaaccg atatgttatt catgcagtct tctgagttgc caacaacagc gtattgtgcc
     9601 tgtaaaaagc cgggcagtag aagaaatagc aataaaataa ttttcgattt caaaacaatt
     9661 ttaac
//
LOCUS       DAOOOL010000100         9193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_711_length_9193_cov_58.333005, whole genome shotgun sequence.
ACCESSION   DAOOOL010000100 DAOOOL010000000
VERSION     DAOOOL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_711_length_9193_cov_58.333005"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..620
                     /locus_tag="PKU82_08580"
     CDS             156..620
                     /locus_tag="PKU82_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ82967.1"
                     /translation="MVLKIKIERTTSKDINYIKLVAELDKDLKAKNGDKNDFFAQYNK
                     SGNINHVIVAYINEKPVGCGAFKEYNSTTVEIKRMYVKTELRGQGIALTILNALEKWA
                     SELNYNECILETGDKMAEAIGLYQKHNYKRIKNYGQYENVASSFCFSKSIIT"
     gene            676..1266
                     /locus_tag="PKU82_08585"
     CDS             676..1266
                     /locus_tag="PKU82_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOZ82968.1"
                     /translation="MNKTRLEAFSDGVLAIIITIMVLELKVPHETSIKALLILWPVFF
                     SYIISFIYVGIYWGNHHHLLHTIHHVNGKVIWANMGLLFCLSLLPFTSAWMGENIPEK
                     IPLMLYSINLAVCAVAFYLLQMCVLPQYTTTSKLIDAVKKQKTKGMISLGIYIAASVA
                     ALILPWLSVVLIIIPAILWVIPDKNIEAALSEENNK"
     gene            1518..2480
                     /gene="ric"
                     /locus_tag="PKU82_08590"
     CDS             1518..2480
                     /gene="ric"
                     /locus_tag="PKU82_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOZ82969.1"
                     /translation="MQTEVDNVLNVTLLEPRMKHPTIFARFDELNPGESLIIHNDHDP
                     KPLYYQLLGERGNIFTWEYLEQGPQWWKVKISKRISGESDETLGEIVAKDMHKAQIFK
                     KYGIDFCCGGKKTVKQACAEKGIDVTIIEQELQKAGKVVSNRPLPYNEWNLDFLADYI
                     VNTHHTYVKNNLPEISGYAAKVARVHGHNHPELLRINQLVEAISSEFSSHMAKEEQVL
                     FPYIKRMVEAKNNKETIQQSSFGSVKGPINMMEIEHESAGKNMSEIRTLSNDFTLPED
                     ACASYSLLFRLLEEFEEDLHTHVHLENNILFPKALDLEKELVTK"
     gene            2748..3071
                     /locus_tag="PKU82_08595"
     CDS             2748..3071
                     /locus_tag="PKU82_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOZ82970.1"
                     /translation="MENNIKPAFQTWASGRVSGFYGKELLNLNNGTVKLVKVDPHAQY
                     PEHIHPDKTEYAFVLEGNPEFFIEGNHYSGQPGEFFIFPSAKKHAIQNNTSSECTLLI
                     GAITV"
     gene            3130..3933
                     /locus_tag="PKU82_08600"
     CDS             3130..3933
                     /locus_tag="PKU82_08600"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOZ82971.1"
                     /translation="MTINANTKIAAILKQHPDALDAIVSISPKFEKLRNPLLRKIMAG
                     RTSLAMASKIGGCSVNDFFVKLQPLGFEIDSTAQVIEKPADKMPEVFTTLKKEQIVIL
                     DVRPVLAAGNDPLNLILEKVNSLEAGKCLKIINTFEPTPLIIMLEKKGFVTYTDTISD
                     ELIETYFYKKSDNKNEINPVVNASGGWDEIMQQYNNKLQTIDVRAMEMPLPMLTILDA
                     LEKLPSNHALYVYHKRIPVFLLPELAEKKFDYRIKEISDGEVHLLIFKS"
     gene            3945..5225
                     /locus_tag="PKU82_08605"
     CDS             3945..5225
                     /locus_tag="PKU82_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HOZ82972.1"
                     /translation="MIISDSIKTTSYKVVLPFYIYAAFAFLLSAILLLTSSSAFTDHY
                     FQPHILAITHLMALGWGTMIILGASHQLVPVLIEGKLYSNALAYASFILAGIGIPLLV
                     YGFYVFNMHNPAKWGGRFILLSIIAYLINLGVSMSKSKKENIHALFVFTSALWLFLTG
                     ALGLALVYNFTYNMFPHDSLHYLPLHVHAGVIGWFLMLIIGVGSRLIPMFLISKYTNT
                     KLLKWIFVLINGALISYILIFYFVKITEVILLPWLMLFTGIALFIFYCYSAFKHRIRK
                     QVDEPMKVSLLAVMLLMIPLLLLLISIVSAVIFSQEDIALSLSYGFLIFFGWITAIIL
                     GMTFKTLPFIVWNKVYHKRSGIGKTPNPKDLFSNTVFKLMSVAYIAGLLLFVTGSLMI
                     HLLLIKCGAVLLLMAAALYVFNVLKVITHKPQQL"
     gene            5222..5545
                     /locus_tag="PKU82_08610"
     CDS             5222..5545
                     /locus_tag="PKU82_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOZ82973.1"
                     /translation="MNVKTNNNLKCTVALAALEHVDDPEIGLNVVDLGLIYEVNFDEN
                     EKKITCIMTLTTEFCPMGESITENARNAVQQVFPEYSIDLNLTFDPSWNPEMISEEGQ
                     KFLGR"
     gene            5548..5979
                     /locus_tag="PKU82_08615"
     CDS             5548..5979
                     /locus_tag="PKU82_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOZ82974.1"
                     /translation="MLSLSCKAGIKAVIFLGSRFESGDKSGIKEISKHINENEHTVGK
                     ILQKLVKNNIIKSVKGPNGGFYITQKQKSQPIINIVEAIDGKDVFKQCGLGLTKCSES
                     RPCPFHNDFKPVRELFKNMCLEKKVSDLYQNVNNGLSYLVG"
     gene            complement(6119..6331)
                     /locus_tag="PKU82_08620"
     CDS             complement(6119..6331)
                     /locus_tag="PKU82_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ82975.1"
                     /translation="MPIVVNLDVMMAKRKVSLNELSERVDLTLSNLSILKTGKAKAIR
                     FSTLEAICKALDCQPGDILEFANTEK"
     gene            complement(6335..6868)
                     /locus_tag="PKU82_08625"
     CDS             complement(6335..6868)
                     /locus_tag="PKU82_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOZ82976.1"
                     /translation="MANTKFIFQALHVIAWIIFVGLCIEAGGIIVNFIFSIYNPEFVS
                     KLYQKLDLSELYNSNKWIFYSMYSFIIVIALLKVFLFYTVIMLLLKLDLSKPFSSFVS
                     TQISQISYYTLSIGIICLIAAHTTKNLEHHGYVVERLFQFWNDGQAFILMSAVVYVIA
                     TIFKKGVELQNENDLTV"
     gene            complement(7095..8087)
                     /locus_tag="PKU82_08630"
     CDS             complement(7095..8087)
                     /locus_tag="PKU82_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOZ82977.1"
                     /translation="MTHALIAIIILALLFDFINGFHDAANSIATVVSTKVLSPLTAVI
                     WAASFNFLAYWIFKLKVADTVAKTVTPDSVTLMVVGAGLVAAIIWNLLTWWWGIPSSS
                     SHTLIGGFAGAGIANAGSFSALQVDKVMPTVYFIILAPIIGMVMSWIISVITLHLCRK
                     SNVSQVDRTFRKLQLLSSAAYSLGHGGNDAQKVIGIVGAAMLSHGMISDIDHIPEWVP
                     LSCYSAIALGTMFGGWRIVKTMGQRVTKLTPFEGFSAEAAGAFTLFFTQHFKIPVSTT
                     HTITGSIIGVGLTKRISAVRWGVTLQLIWAWILTIPVSGALAAIIFMIARKVFS"
     gene            complement(8092..8730)
                     /locus_tag="PKU82_08635"
     CDS             complement(8092..8730)
                     /locus_tag="PKU82_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOZ82978.1"
                     /translation="MSFIQYFIPQDKKFFPLFDQAALNLQEAGKTMCQLVTSTDADQR
                     KQLIREIERLEHRGDEITHEIFKELSRNFITPFDREDIHRLVSSIDDILDYIHGSSKR
                     IDLYKVKEFSSDMVKLSELLQTQTEELRRVIYELKNKKNMRNISESLVLINSIENHAD
                     DIFDNAVARLFETETNAVEIIKTKEILSALETATDMCEDAANVIDSIIVKMA"
     gene            8841..>9193
                     /locus_tag="PKU82_08640"
     CDS             8841..>9193
                     /locus_tag="PKU82_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ82979.1"
                     /translation="MNITFYGAARNVTGSKHLIETESGKKILLDCGFFQNRGKDNDRL
                     NRNFSFDPQQIDLMILSHAHIDHSGNIPNLVKQGFNKTIFTTQATIDLCEVMLADSAY
                     IQSGDIEYINRRRKR"
BASE COUNT         3226 a         1523 c         1533 g         2911 t
ORIGIN      
        1 tacaaactta aagttgtttt ctgaaaaata tattaataaa taaataaatt aaaagatagt
       61 aaaataaaat gcaatcataa ataaattaca tcaatctttt tagttacttt gaatgctgtc
      121 ttcacatttt cacagcaata gtttacataa gtaatatggt gttaaaaatt aaaatagaaa
      181 gaacaacatc taaagatatt aactatatca aattagtagc agaattagat aaagatttaa
      241 aagctaaaaa tggagataaa aatgatttct ttgcacaata caacaaatca ggcaacatta
      301 atcatgtaat agtggcatac attaatgaaa agccggtagg ctgcggtgca tttaaagaat
      361 ataattcaac cactgttgag ataaaaagaa tgtatgtaaa aacagaacta cgtggtcagg
      421 gaattgcatt aacaatctta aacgcactag agaaatgggc atcagaatta aattacaatg
      481 aatgtattct tgaaacagga gataaaatgg cagaagctat tggactctat caaaaacata
      541 attataaaag aattaaaaat tatggtcagt atgaaaacgt tgcaagcagc ttttgtttca
      601 gcaaatcaat aattacctaa atgaataata ctttaaaaac taaattataa ataaatcacc
      661 tgacataaaa tcaaaatgaa caaaacaaga ttagaggcat ttagcgatgg tgtgcttgca
      721 atcatcatca ccatcatggt attagaatta aaagtacctc atgaaacaag tattaaagca
      781 ctacttattc tatggccggt cttcttcagt tacatcatca gttttattta tgttggtatt
      841 tattggggca atcatcatca tttgctgcat acaatacatc atgtaaatgg aaaagtaatc
      901 tgggcaaata tgggtttgct tttttgccta tcgttacttc catttacaag cgcatggatg
      961 ggtgaaaata ttcctgaaaa aattccattg atgctttatt caattaacct tgcagtatgt
     1021 gcagttgctt tctacttact tcaaatgtgt gttttaccgc aatacacaac cacaagtaaa
     1081 ctaattgacg ctgttaagaa acaaaaaaca aaaggaatga tatcgctagg tatttatatt
     1141 gcagcatcag tggctgcgct tatcttacca tggttaagcg tagtattaat tattattccg
     1201 gcaatacttt gggtaattcc tgataaaaat attgaagctg ccctttctga agaaaacaac
     1261 aagtaagatt tatctgagtt aactcattaa actaaataca gtgagttagt ttatctcttt
     1321 taatcaaaca aatgagttga gataaattca attggataat ttaaattacc aaacagcatt
     1381 tatcagattc ttttggctgc aatcaccaaa aactgacgac ttactgacaa aaatcagtaa
     1441 atattaaaag tatttcttta cttttattaa atatattaat tacttttgtg ccattattta
     1501 aacaaacaca tactaatatg caaacagaag ttgacaacgt tttaaacgta acactactag
     1561 aaccaagaat gaaacaccct accatttttg cacgctttga tgaactgaat cctggcgaga
     1621 gtttaattat ccacaacgat catgacccta agcctcttta ttatcagctt ttaggtgaac
     1681 gtggcaacat atttacatgg gaatatctgg agcagggacc gcagtggtgg aaagttaaaa
     1741 tttcaaaacg tattagtggc gaaagcgatg aaacacttgg tgagattgta gccaaagaca
     1801 tgcacaaagc acaaattttc aagaaatatg gtattgactt ttgctgtggt ggaaaaaaaa
     1861 cagttaaaca agcttgtgca gaaaaaggca ttgatgttac aatcattgag caagaattgc
     1921 agaaagcagg aaaagtagtt tccaacagac cattaccata caatgaatgg aatcttgatt
     1981 ttctggcaga ttatattgtg aatacacatc acacttatgt aaaaaacaat cttcctgaaa
     2041 tcagcggata tgcagcaaaa gttgcaagag tacatggtca taaccatcct gaactattaa
     2101 gaataaatca gttggttgaa gcaatcagct cagagttttc atcacatatg gcaaaagagg
     2161 agcaagtact gtttccttat attaagcgca tggttgaagc aaaaaataat aaagaaacta
     2221 tccagcaatc atcatttggt tcagtaaaag gccctatcaa tatgatggag attgagcatg
     2281 aatcggcagg taaaaatatg agtgaaataa gaacacttag caatgacttt actttgccgg
     2341 aagatgcttg tgcaagttac agtttgctat tcagattgct tgaagaattt gaagaagatt
     2401 tacatactca tgtgcactta gaaaacaaca tactttttcc aaaagcactt gatcttgaaa
     2461 aagaactagt aacaaagtaa aagattaaac ttattagtat tattttatag ttcatttaat
     2521 tccggtagtg tttcattaat ctgacataga cgaaaagtca ataaatacag agcagcagaa
     2581 aggtatggtt tgttatgttt gtgataccag atgcaatttt cgctctgtat ttatgtcaga
     2641 taatttaata cttaaattaa ttcaaacttt ttaagctaaa gcttaaaaag tttatttttc
     2701 cagtctcaaa gtatcctgaa cactatattt ttaaaaaaaa tcatacaatg gaaaataata
     2761 taaaacccgc ctttcaaaca tgggcatcag gaagagtgag tggattttac ggcaaagagt
     2821 tacttaatct caacaatggc actgttaagc tagtaaaagt agatccacat gcacagtatc
     2881 cggaacatat tcatccggac aaaacagaat atgcattcgt tttagaaggg aatcctgaat
     2941 tttttattga aggcaatcat tattctggtc agccgggtga attttttatt ttcccatcag
     3001 caaaaaagca tgctatacaa aataacacat cttcggaatg tacattactg attggtgcta
     3061 ttacggtttg aaatattgaa tccaaccatt acctgaataa aataatgtac tgttataaaa
     3121 tattttcaga tgactataaa tgctaatact aaaattgctg ccatattaaa acaacatccc
     3181 gatgcacttg atgcgatagt aagtatcagt cctaagtttg agaagctacg caatccgtta
     3241 ttaagaaaaa ttatggcagg acgtacaagt ttggctatgg catcaaaaat aggcggatgt
     3301 agtgtaaatg acttctttgt aaaactacag ccattaggtt ttgagattga cagcacagct
     3361 caggttattg agaagccggc cgataagatg cctgaggttt ttaccacgtt aaaaaaagaa
     3421 cagatagtta tacttgatgt cagacctgtg ctagctgctg gcaatgatcc actaaacctt
     3481 attcttgaaa aagtcaacag tcttgaagcc ggaaaatgtt taaaaataat aaacactttt
     3541 gagcctactc cactcatcat catgttagaa aaaaaaggtt ttgtaacata tacagacacc
     3601 attagtgatg aactcattga aacatatttc tacaaaaagt cagacaacaa aaacgaaata
     3661 aatcctgtag ttaatgcttc gggtggatgg gacgaaatca tgcagcaata caataataaa
     3721 cttcaaacta ttgatgtgcg tgctatggag atgccacttc cgatgcttac aattttagat
     3781 gctttagaaa aactaccatc caatcatgca ttatatgttt atcataaacg tattcctgta
     3841 tttttgctac ccgaattagc tgaaaagaaa ttcgattacc gaataaaaga aatatctgat
     3901 ggtgaagtgc acctgcttat tttcaagtcg taatgcatta aagtatgatc atttctgatt
     3961 ccataaaaac aacatcctat aaagtggtgt tgccatttta tatttatgct gcttttgcat
     4021 tcttactgtc tgcaatttta ctcttaacat cttcttctgc ctttacagat cattattttc
     4081 agcctcacat tttagccatc acacatctga tggcactggg ttggggtact atgataatat
     4141 taggcgcaag ccatcaactt gttcccgtat tgattgaagg aaaactttat agcaatgcat
     4201 tagcatatgc ttcatttatt cttgcaggaa ttggtattcc gttattagtt tatggatttt
     4261 atgtattcaa catgcataat cctgccaaat ggggtggcag gtttatatta ttatcaatca
     4321 ttgcatactt gattaatctt ggtgtaagta tgtcgaaaag caaaaaagaa aatattcatg
     4381 ctttatttgt ttttacatcg gcattgtggt tatttcttac gggtgctctg ggtcttgcat
     4441 tagtatataa ctttacatac aacatgtttc cgcatgattc tttacattat ttaccgcttc
     4501 atgtgcatgc aggtgtgatt ggttggtttc tgatgctgat aataggtgta ggctcaaggc
     4561 tgatacccat gtttttaatt tctaaatata ccaacacaaa actcctcaaa tggatttttg
     4621 tattgataaa cggtgcattg atatcctata ttctgatatt ttactttgtg aaaatcactg
     4681 aagtaattct attaccttgg ctgatgcttt ttacaggaat tgcacttttt attttctatt
     4741 gttattcagc ttttaaacat cgtatcagaa aacaggttga cgaaccaatg aaagtttcgc
     4801 tattggctgt aatgctcctt atgataccat tgttgttgct attgatttct attgtttcag
     4861 ctgtaatttt ttcgcaggaa gacattgcct tatctttaag ttatggcttt ctgatatttt
     4921 ttggatggat aacggcaatt attctgggaa tgacatttaa aacacttcca tttattgtgt
     4981 ggaacaaagt ttatcataaa cgttcgggca ttggaaaaac acctaaccca aaagatctat
     5041 ttagtaatac tgtatttaaa ttaatgagtg tggcctatat tgcaggacta cttctatttg
     5101 ttacgggtag tttgatgatt catctgttat taattaaatg tggtgctgta cttttactaa
     5161 tggcagccgc cttatatgtg tttaatgttt taaaagtaat tacacataaa cctcaacaat
     5221 tatgaatgta aaaacaaata acaatttaaa atgtactgtt gcacttgctg cacttgaaca
     5281 tgttgatgat cctgaaatag gacttaatgt tgttgactta ggattgattt acgaagttaa
     5341 tttcgatgag aacgaaaaaa agattacatg tattatgact cttactactg agttttgccc
     5401 tatgggcgaa tccattactg aaaatgcaag aaatgcagta caacaggtgt ttccggaata
     5461 ttcaatagat ttgaacctga cttttgatcc atcatggaat cctgaaatga tttcggaaga
     5521 aggtcaaaaa tttttaggac gataattatg ttgagtttaa gttgtaaagc aggaattaaa
     5581 gctgttattt ttcttggatc cagatttgaa tctggtgata agtccggcat taaagaaatt
     5641 tcaaaacaca ttaatgaaaa cgaacatacc gttggaaaaa tattacagaa gttagttaag
     5701 aacaacatca ttaaaagtgt taaaggacca aatggaggtt tttatataac acaaaaacaa
     5761 aaaagccaac ctataataaa tattgttgaa gctattgatg gcaaggatgt atttaaacaa
     5821 tgtggtttgg gattaacaaa atgttcagag tcgcgcccct gcccttttca caatgatttt
     5881 aaaccggttc gggagttgtt taaaaatatg tgtctcgaaa aaaaagtaag tgacctttat
     5941 cagaatgtca ataacgggct ttcttactta gtaggttaga aaataaaatt ataacttagg
     6001 aggcatttac gtagttttaa gtactaatga ctgctttcaa attaacagaa taaaatttaa
     6061 taatacgata ttgctaagtt gatttacgat aagcaacctc tattcgtaat gtatcaattt
     6121 atttttcagt gtttgcaaat tcaagaatat ctccgggctg gcaatctaat gctttacaaa
     6181 ttgcttctaa agtactgaaa cgaatagcct tggcttttcc ggtttttaaa attgacagat
     6241 tggataaagt taagtcaaca cgttcagaaa gctcgttgag cgaaactttt cgcttcgcca
     6301 tcataacatc taaatttaca actatcggca tagcttatac agttaaatcg ttttcattct
     6361 gaagttcaac ccccttctta aaaatagtag caattacata aactactgca gacatcagaa
     6421 taaatgcctg accatcattc caaaattgaa ataaacgttc aactacataa ccatgatgct
     6481 ctaaattttt agttgtatga gctgctatta aacatattat cccaattgaa agcgtataat
     6541 aactgatttg tgaaatttga gttgatacaa aactgctgaa cggtttggac aaatcaagtt
     6601 taagcagtaa catgattacc gtgtaaaata aaaacacttt caacaatgca ataacaatga
     6661 tgaaactata catactgtaa aagatccatt tgttgctgtt gtacaattca cttaggtcga
     6721 gcttttgata aagtttactt acaaattcgg gattgtagat actgaaaata aaatttacaa
     6781 taatgccgcc tgcttctata caaagaccaa cgaagatgat ccaggcaatg acatgcaatg
     6841 cctgaaagat aaatttagtg ttcgccatat ttaattgtga tttacttagg caaaaataat
     6901 atatatttat cgtttatcaa taaatatatt tcctttttca ataatattag tttaactaat
     6961 tgaaaaccaa aatcataaaa ttcaacgctt attctgtcaa tccattatag tttgattaca
     7021 gctacagtca aatacaaagt attcagaaag actacttgag ttactcaaaa aaatttctta
     7081 taagagtgta tctattaact gaaaacttta cgtgctatca taaaaatgat agcagccaaa
     7141 gcaccggaaa caggaatagt tagaatccat gcccagataa gttgaagtgt tactccccaa
     7201 cgaacagctg aaattctctt agtgagtcca acaccaataa tagaacctgt aatggtatgt
     7261 gttgtactta caggtatttt aaaatgttgc gtaaaaaata aagtaaatgc ccctgctgct
     7321 tcagcactga atccttcaaa aggagtgagc tttgtgacac gctgtcccat ggtttttaca
     7381 atacgccatc cgccaaacat agttccaagt gcaatagctg aataacaaga aagtggaacc
     7441 cactcaggaa tatggtcaat atcgctaatc attccatgcg aaagcatggc cgcaccaaca
     7501 attccaatta ctttttgtgc atcgttgccg ccatgaccca aactataagc agcagatgac
     7561 aataactgta attttctaaa tgtcctatcc acctgtgata catttgattt cctgcataaa
     7621 tgaagtgtaa tcactgatat aatccaagac attaccattc caataatagg agccagaata
     7681 ataaaataaa ctgttggcat taccttatcc acttgcaatg cactaaaact tccggcatta
     7741 gcaatacctg ctcctgcaaa accaccaata agtgtatgtg atgaactgct tggaattccc
     7801 caccaccagg tcagtagatt ccagatgatt gctgcaacaa gacctgctcc gacaaccatc
     7861 aacgtaacac tatcaggtgt aacagtcttg gcaacggtat cagctacctt aagtttaaaa
     7921 atccagtatg caagaaaatt aaatgatgct gcccagataa ctgctgtcaa tgggcttagt
     7981 acttttgttg atacaactgt tgcaatagag ttggctgcat catgaaaacc attaataaaa
     8041 tcgaaaagta acgcaaggat gattattgca ataagtgcat gagtcatatc atcaggccat
     8101 tttaactatg atagaatcta tgacattggc agcatcttcg cacatatcgg ttgctgtttc
     8161 caatgccgat aaaatttctt ttgtttttat aatttcaaca gcattggttt ccgtttcaaa
     8221 taatcttgct actgcattat caaaaatatc atcagcatga ttttctattg agttaatcag
     8281 aaccaatgat tcagaaatgt tacgcatatt cttcttattt ttcaactcat agataacacg
     8341 tctcagttct tctgtttgtg tttgaagtaa ctctgaaagt ttcaccatat cagatgaaaa
     8401 ttcttttact ttatataagt caattctttt tgatgatcca tgtatgtaat caagtatatc
     8461 atcaatagaa ctcactagcc tgtgaatgtc ttctctgtcg aaaggtgtaa taaaattacg
     8521 gctcaactct ttaaaaatct catgtgtaat ttcatcgcca cgatgttcaa gtctttctat
     8581 ttcacgaatt agttgtttgc gttgatcggc atcggtagaa gtaaccaact gacacatagt
     8641 tttaccggct tcctgcaggt ttaatgctgc ctggtcaaat agtggaaaaa actttttatc
     8701 ctgagggata aaatattgaa taaatgacat aaaccgatgt ttatttaaaa ggattatgcc
     8761 gcaaaactaa aaacactgag ctaaaactga agtaatgttg ataaaaagtt attgaaaaaa
     8821 aatattattt ttgaatcctt atgaatataa ctttttatgg tgctgcccga aatgttaccg
     8881 gtagtaaaca cctgatagag actgaatcag ggaaaaaaat actactcgat tgtggttttt
     8941 ttcagaacag aggtaaagat aatgatcgcc tgaatcgtaa ttttagcttt gatcctcagc
     9001 aaattgacct gatgattctg tcacatgcac acattgatca ttcaggaaat attccaaact
     9061 tagtcaagca gggatttaac aaaacaatct ttacaacgca agctactatt gacctttgcg
     9121 aagtaatgct tgccgatagt gcctatattc aaagtggtga tatcgaatat atcaacagaa
     9181 gacgaaaaag aga
//
LOCUS       DAOOOL010000101         9056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_727_length_9056_cov_85.526386, whole genome shotgun sequence.
ACCESSION   DAOOOL010000101 DAOOOL010000000
VERSION     DAOOOL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_727_length_9056_cov_85.526386"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /gene="pepT"
                     /locus_tag="PKU82_08645"
     CDS             <1..1153
                     /gene="pepT"
                     /locus_tag="PKU82_08645"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOZ82980.1"
                     /translation="PSSKKQLDLSRLLESELKAMGIDAALDEYGYVYATIKGNSKKKV
                     PVICFCSHVDTAPDCSGTNVKPILHKKWNGEDIVLPDDNNVVISSKKHPYLLEKIGDD
                     IITASGLTLLGADDKAGVSIIMDFAQYLLNRKDIVHGDIRILFTPDEEIGRGVNKVDM
                     KKLNADFGYTLDGGECGTLEGENFSADSCTVTFNGISAHPGYAKGKMVNAQKVLAHFI
                     TLLPKEMSPENTEAYEGFVHPVSISGGLETATVAFILRDFVTAKLTEQKQLLNKLAEQ
                     TIKQFPGANFTISSKEQYRNMDEVLQKHPEVMENACEAYKRSDINCERKNIRGGTDGA
                     RLSFMGMPCPNIFTGEMGIHSKEEYVSVQDMNKAVEVLVNLVQVWEQRA"
     gene            1397..3457
                     /locus_tag="PKU82_08650"
     CDS             1397..3457
                     /locus_tag="PKU82_08650"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOZ82981.1"
                     /translation="MKKQITLLFGIMCFALAVNAQYYYFPHINAGQNPGGLNTDDEYP
                     VAGGQPAGWTSIHPGSATTPQWSTTVTIPFAFSFNGAAVTNYKVSTTGVLTFTTGAAT
                     PPPVANVALPSALVPDNSVCCWGISGSGTNDNICTKTFGTAPNRQHWISFTSYTDPGN
                     TAYTYYSIVLEESTNKIYVVDQRNGGTALTLTIGVQINATTAVSVAGSPNISQYAGAD
                     PSPADNSYWEFIYGSQPANDVALSSLNYTQYVTTPTNVTLSGVITNLGSSAITTVDLK
                     YQLNGTTYTDTKTGLNIAPLGTYNFTHATPINASAVGTYSFNFWADLAGDANHINDSL
                     NGVINYLPFLPTKRIVFEEATGTWCGWCPRGAVYMDSLRNLHGQEVMLIAVHNGDPMT
                     VAAYDSWMGTKISGYPSGLVDRKDLDVDPSDFITEYNSLISDIPPCDVNVTATYNSST
                     NVGTFTVSPQFAASLNGDYRINCVVTEDNLTGTTSSWSQANYYSYQTANMPLQGAGHD
                     WQNETDPVPAANMEYDHVARAILGGNDGVQGSLPANITSGGTYSWTFNYNIPATQNVS
                     NLIIIGWVSDFATGRILNANSATVTTGIHNLTGTYFNMNVTPNPITSDYGQVKVNLKK
                     NADVVFEIIDVLGNIVRTNYQSQMTAGEYIYPVNFTGLSNGIYTVRMNASGETLTQKV
                     VLNK"
     gene            complement(3605..4687)
                     /gene="aroC"
                     /locus_tag="PKU82_08655"
     CDS             complement(3605..4687)
                     /gene="aroC"
                     /locus_tag="PKU82_08655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOZ82982.1"
                     /translation="MAGNTFGTLFRITTFGESHGPLIGVVIDGCYSGVQLDLELIRTQ
                     LSRRKPGQSAITTQRMEDDEPEIVSGVFNGITTGAPLTILIRNKDQRPTDYDALKDAY
                     RPSHADYTYEKKYGIRDHRGSGRASARETAARVAAGAVAIQMLNAANISIDAYVSRIG
                     NVSIPENVSIDTTQTYNNILRCPHEATAQLMQKSIEETAQTGDTLGGIISCIISGVPA
                     GLGEPVFDKFHAQLGKAMLSINAVKGFEIGSGFNGTSQMGSQQNDVFEIDKDGKVFTR
                     TNNSGGVQGGITNGMDITFNVAFKPVSTLMKSQQSINLQNEKVVIQPGGRHDVCVVPR
                     ATPIVEAMAAITTLDFLMIANAAKCK"
     gene            complement(4689..4958)
                     /locus_tag="PKU82_08660"
     CDS             complement(4689..4958)
                     /locus_tag="PKU82_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ82983.1"
                     /translation="MCFREETDYSFFLDKLYVDSELQHKNLGSLLLEAMLSSLPEDII
                     IRLQVNRSNYKAINFYFKKGFVIERAEDFDIGNGFFMNDFVMIKK"
     gene            complement(4959..5357)
                     /locus_tag="PKU82_08665"
                     /pseudo
     CDS             complement(4959..5357)
                     /locus_tag="PKU82_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
     gene            complement(5359..5838)
                     /locus_tag="PKU82_08670"
     CDS             complement(5359..5838)
                     /locus_tag="PKU82_08670"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ82984.1"
                     /translation="MHIRQASTDDCSAISALANSIWHSHYPGIISMQQIDYMLSKMYS
                     VEKLNHLLESGSVILVAEEDEKLLGYICFREETDYSFFLDKLYVDSELQHKNLGSLLL
                     EAMLSSLPEDIIIRLQVNRSNYKAINFYFKKGFVIERAEDFDIGNGFFMNDFVMIKK"
     gene            complement(5842..6405)
                     /locus_tag="PKU82_08675"
     CDS             complement(5842..6405)
                     /locus_tag="PKU82_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOZ82985.1"
                     /translation="MKILVIDNYDSFTYNLVHYIEKAAGVQPTVWRNNSHSVDEVAAF
                     DKICISPGPGVPSQAGFTKEVIKRFTSEKSILGVCLGHQAIAEVFGGSLRNLQQVLHG
                     IVRPVNVLVQDVLFENMPSSFPAARYHSWVVDQTGSQLQVIAGDDIGEVMAIKHIQYP
                     VYGVQFHPESIMTEHGLQLIYNWIYKT"
     gene            complement(6441..7148)
                     /locus_tag="PKU82_08680"
     CDS             complement(6441..7148)
                     /locus_tag="PKU82_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ82986.1"
                     /translation="MADQNLRILLVEDEQTLSETIKLNLEMEGYYVNAVGDGKTAIKV
                     FKEARYNLVILDVMLPEISGFTVCEAIRLENLDVPILFLSAKNHPNDRITGLKLGGDD
                     YLTKPFNLEELLLRVHNLIRRSTANKDAASLSTYNLNDNQINFAQLEIKRADGTITHL
                     TKKESLLLKLLIEKRNEVVSREHILETVWGYDIYPSTRTIDNFIVTFRKYFEPDPSNP
                     KYFISVRGVGYKFTDQD"
     gene            7197..8747
                     /locus_tag="PKU82_08685"
     CDS             7197..8747
                     /locus_tag="PKU82_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82987.1"
                     /translation="MLRNYSLSQSGKYWIWSIFYSLLFLAVYGYKFNCSDLEEHLPAV
                     YKMLNPQLYQGDYVTEFLASNFSVRYFYNHTLYYPGLVFPLSSVSFTLMLILLTVFIF
                     YIQKTSFVIFKNHITASITPFIAIILFNKITIGGNSVSDNMLTCTVFANTLCMTAYYY
                     GLSSRLRIAALCCGLATWYQPLVGLQVFLLLLLMHLVINKFRVKEIGTTIIFYFLSAS
                     PMLIPLFNRQFFAESAAPDKLFYYVLYVFRNPHHYLPSFFPIADYIKSGILFLISGLL
                     FFRFPTKYNRQIMVIISGIVGGMIFYYLIFDVGGLMVVGKLQWFKTSVWLTIFCSFYI
                     AHFIAKQNLIQNILNYKWVSACVIALSIFIFYLITHASLLPLEKLKSRYAVGQFKKTD
                     LTLMHEWIRENTTDTTKILSFPDDDSFLCEAQRPTPVAWKAIIHEPWFLKTWYYNFTT
                     DYNLSRKIEWKNINQREKASSIFLGIQDKPTNASYSMILFNANKLPEQHKGKIIHRQG
                     EYTLWQWN"
     gene            complement(8752..>9056)
                     /locus_tag="PKU82_08690"
     CDS             complement(8752..>9056)
                     /locus_tag="PKU82_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ82988.1"
                     /translation="NSDKLKPASLIQLDALVGILKKYDAANLSIEGHTDNKGDDAYNL
                     NLSQKRCDAVKSYLMSKGIFESRLTATGFGETKPIAGNDTPEGRAKNRRVELKTSY"
BASE COUNT         2877 a         1724 c         1585 g         2870 t
ORIGIN      
        1 gcccagcagc aaaaaacaat tagacttaag caggttgctt gagtcagaat taaaagcaat
       61 gggcattgat gctgcacttg atgagtatgg ttatgtctat gctacaatta aaggtaactc
      121 aaaaaagaaa gtacctgtaa tttgtttttg ttcgcacgtt gacactgcac ctgattgcag
      181 tggcacaaac gtaaagccta tcctgcataa aaaatggaat ggtgaggata tcgttttacc
      241 tgacgacaac aatgttgtta tcagcagtaa aaaacaccct tatctactcg aaaaaattgg
      301 tgatgacatc atcacagcct caggacttac gctgttaggc gctgatgata aagcaggtgt
      361 ttccatcatc atggattttg cacagtacct tttaaacaga aaggatattg ttcatggtga
      421 tataagaatt ttatttacgc ctgacgaaga aattggtcgt ggtgtaaaca aagtggatat
      481 gaaaaaatta aacgctgact ttggttatac cctcgatggt ggagaatgtg gtacattaga
      541 aggtgaaaat ttttcggctg actcatgcac agtaacattt aatggcatca gtgcacatcc
      601 gggttatgcc aaagggaaaa tggttaatgc acaaaaagta ttagcgcact ttatcacgct
      661 tttaccaaaa gaaatgagtc ctgaaaatac agaagcgtat gaaggttttg tacatcctgt
      721 cagcattagt ggaggattag aaacagcaac tgtcgctttt attcttcgtg attttgttac
      781 tgctaaatta accgaacaaa agcaactgct taacaagttg gcagaacaaa caatcaaaca
      841 atttcccgga gcaaatttta ctatcagtag taaagaacaa taccgcaaca tggatgaagt
      901 tcttcaaaaa catccggagg taatggaaaa tgcctgcgaa gcctataaac gcagtgacat
      961 caattgtgaa cggaaaaaca tcagaggcgg aacagatgga gcacgattat catttatggg
     1021 catgccttgt ccgaatattt ttacaggaga aatgggtatt cacagtaaag aagaatatgt
     1081 aagtgttcag gatatgaata aagctgttga ggtgttggtt aatttagttc aagtttggga
     1141 acaacgagcc tgacagtagt cccttcctct tattcagttc gataaattta ctaaagaaat
     1201 ttaagcttgg tttgaactaa ttagttttca cttagttggt attttaatgc aatcgtatca
     1261 gtaatacaaa ctgttataga ctaactttaa acaaccgtcc attgccttcg tagagctaaa
     1321 acaattattt aactaaaagt cttaatttta cgttgcgaaa aatgataatt tcgcagtcaa
     1381 ttaaatttaa ataaaaatga aaaaacaaat tactcttcta tttggcataa tgtgttttgc
     1441 attagctgta aatgcacagt attattattt tccacacatc aatgctgggc aaaatccggg
     1501 tggccttaac acagatgatg aatatcctgt tgctggaggc caacctgcag gttggacatc
     1561 cattcatccc ggaagtgcta ccactccaca atggtcaaca actgtaacaa ttccgtttgc
     1621 attttctttt aacggtgcgg cagttacaaa ttacaaagtt tccacaacag gagttttaac
     1681 ctttacaaca ggtgcagcta cacctcctcc ggttgccaac gttgcattac cttcagcttt
     1741 agtacctgat aattcagttt gttgttgggg aatttcagga agtggtacaa atgataatat
     1801 ctgtactaaa actttcggaa cagcaccaaa ccgtcagcac tggatttcat tcacttccta
     1861 tactgatccg ggcaacactg catatactta ttatagtatt gtattagaag aatcaacaaa
     1921 taaaatttat gtagttgatc aaagaaacgg tggaacagcg ttaacactca caataggtgt
     1981 tcagattaac gccactacag ccgtttctgt tgcaggttct ccaaacattt cacaatatgc
     2041 aggtgcagat ccatcacctg ctgacaacag ttattgggaa tttatctatg gttcacagcc
     2101 agcaaatgat gtagcattat cttctttaaa ctatacacaa tatgttacaa caccaactaa
     2161 cgttacatta tcaggtgtta ttacaaatct tggttcaagt gcaataacta cagttgatct
     2221 taaataccaa ctcaatggca caacttatac cgacacaaag acaggattaa atattgcacc
     2281 ccttggaaca tataatttta ctcatgcaac tcctataaat gcaagtgcag taggaactta
     2341 ttctttcaac ttttgggcag atttagccgg agatgcaaac catattaatg atagtttaaa
     2401 tggtgttatt aactatcttc cgtttttacc aacaaaacgt atagtatttg aagaagccac
     2461 aggtacttgg tgcggatggt gtccacgtgg tgctgtttac atggattcgc tccgcaactt
     2521 acatggccag gaagttatgt tgattgctgt tcacaatggc gatcctatga ctgttgccgc
     2581 ttatgattca tggatgggaa ctaaaatcag tggctatcca agcggattgg ttgatcgtaa
     2641 agatttagat gttgacccat cagattttat cactgaatac aacagcctta tttccgatat
     2701 tcctccttgt gacgttaatg tgactgcaac gtataattca agtaccaatg ttggaacgtt
     2761 tactgtaagc ccacaatttg cagcatcatt gaatggcgat tatagaatta actgtgttgt
     2821 tacagaagat aatttaaccg gcacaacttc atcatggagt caagcaaact attatagcta
     2881 tcaaacggca aatatgccgc ttcagggtgc cggacatgat tggcagaatg aaacagatcc
     2941 tgtaccagca gccaacatgg aatatgatca cgttgctcgt gcaattcttg gtggaaatga
     3001 tggtgtgcaa ggttcattgc ctgcaaacat aacttccggt ggtacttaca gttggacatt
     3061 taattataat attccggcaa ctcaaaatgt aagcaatcta atcatcattg gatgggtttc
     3121 tgattttgct accggtagaa tcttaaatgc aaacagtgca acagttacta caggaattca
     3181 taacttaaca ggaacatatt tcaatatgaa tgtaacacca aatccgataa catctgatta
     3241 tggtcaggtg aaagttaatt tgaagaaaaa tgcagatgta gtattcgaaa ttattgatgt
     3301 attaggtaat atcgttcgca ctaattatca atcacagatg acagcaggtg aatatattta
     3361 tcctgtaaat ttcacaggtt taagcaatgg tatttatacc gttagaatga atgcatccgg
     3421 tgaaacatta acacaaaaag tagtattgaa taaataaaat tatatcggta gatttaccgt
     3481 ataagattaa aatcgaaaag gcagtttcaa tataaaaatt gaaactgcct ttttctttgc
     3541 tagtcaataa tctttatgag taaaccttaa tacctgaata ccgtaaacat taaaattata
     3601 tcaactattt gcacttagca gcattggcaa tcattaaaaa atcgagtgtt gtaattgctg
     3661 ccattgcctc tacaattggt gttgcacgtg gcacaacaca aacatcatga cgtcctccgg
     3721 gttgaataac cactttttcg ttttgtaagt taatgctttg ttgtgacttc atgagtgttg
     3781 acactggctt aaatgctacg ttaaaagtaa tgtccatacc attggtaatt ccaccctgca
     3841 ctcctccgga gttgttggtc ctagtaaaaa ctttcccatc cttgtcaatc tcaaaaacat
     3901 cattctgctg tgaccccatt tgcgaagttc cgttaaaccc cgaccctatt tcaaagcctt
     3961 taacagcatt aatactaagc atggccttac ctagttgtgc atgaaattta tcaaatacag
     4021 gttcacctaa cccggcagga acaccactaa taatacagga tattattcct cctaaagtgt
     4081 ctcctgtctg tgcagtttct tcaatacttt tttgcatcaa ctgtgcagtg gcttcatgag
     4141 gacagcgcaa aatattatta taggtttgtg tagtatcaat tgaaacattt tcaggaatag
     4201 aaacattgcc gatacggctt acataagcat caatagaaat atttgcagca tttagcattt
     4261 gtatagcaac agctcctgca gccacacgtg cagcggtttc gcgtgctgat gctcttccac
     4321 ttccgcggtg atcacgaata ccatattttt tctcataggt ataatctgca tgtgatggcc
     4381 tatacgcatc tttaagtgca tcataatctg taggacgttg atctttgtta cgaataagaa
     4441 tcgttaatgg tgcacctgtt gttatcccat taaaaacacc tgaaacaatt tccggctcat
     4501 catcttccat ccgctgcgta gtaattgcag attgacccgg cttgcgtctt gacagttgtg
     4561 ttcttatcaa ttctaaatcc aactgaactc ccgaataaca accgtcaata acaacaccaa
     4621 taaggggtcc gtgtgattca ccaaaagtgg ttattctgaa aagtgttccg aatgtattac
     4681 ctgccatatt actttttaat cattacaaaa tcattcataa aaaagccatt gcctatatca
     4741 aagtcttctg cacgttcaat aacaaaaccc tttttaaaat aaaagttaat tgctttataa
     4801 tttgaacggt taacctgcaa acgtattata atatcttcag gcaaactact caacatagct
     4861 tcaagaagca atgaacccaa atttttatgt tgcaattctg aatcaacata taatttatcg
     4921 aggaaaaaac tgtaatcagt ttcttcacga aaacaaatag cagcggtttc gcgtgctgat
     4981 gctcttccac ttccgcggtg atcacgaata ccatattttt tctcataggt ataatctgca
     5041 tgtgatggcc tatacgcatc tttaagtgca tcataatctg taggacgttg atctttgtta
     5101 cgaataagaa tcgttaatgg tgcacctgtt gttatcccat taaaaacacc tgaaacaatt
     5161 tccggctcat catcttccat ccgctgcgta gtaattgcag attgacccgg cttgcgtctt
     5221 gacagttgtg ttcttatcaa ttctaaatcc aactgaactc ccgaataaca accgtcaata
     5281 acaacaccaa taaggggtcc gtgtgattca ccaaaagtgg ttattctgaa aagtgttccg
     5341 aatgtattac ctgccatatt actttttaat cattacaaaa tcattcataa aaaagccatt
     5401 gcctatatca aagtcttctg cacgttcaat aacaaaaccc tttttaaaat aaaagttaat
     5461 tgctttataa tttgaacggt taacctgcaa acgtattata atatcttcag gcaaactact
     5521 caacatagct tcaagaagca atgaacccaa atttttatgt tgcaattctg aatcaacata
     5581 taatttatcg aggaaaaaac tgtaatcagt ttcttcacga aaacaaatat agcctaaaag
     5641 tttttcatct tcttctgcaa ctaaaattac agaacctgac tcaagcagat ggttcaattt
     5701 ctcgacactg tacattttgc tcaacatgta atcaatctgt tgcatagaga taataccagg
     5761 gtaatgacta tgccaaatgc tatttgctaa tgcgctgatg gcactacaat cgtctgttga
     5821 agcttgcctg atatgcatgg ttcaggtttt ataaatccaa ttatatataa gttgcagtcc
     5881 atgttcagtc ataatgcttt ccggatgaaa ttgaactcca taaacaggat attgtatatg
     5941 ttttatggcc atcacttcgc caatgtcgtc accggcaatt acctgaagtt gactgcctgt
     6001 ttgatccaca acccatgaat gataacgtgc agccggaaat gatgaaggca tattttcaaa
     6061 caacacatct tgtaccaata cattcaccgg gcgtactata ccatgcaaaa cttgttgtaa
     6121 attacgaaga ctgccaccaa aaacttctgc aattgcctga tgacctaaac aaacaccaag
     6181 tattgatttt tctgatgtaa atcgtttgat aacttctttg gtaaaacctg cctgagaagg
     6241 cactcccggc cccggagaaa tacaaatctt atcaaatgca gcaacctcat caacagagtg
     6301 agaattgttt cgccaaactg ttggttgcac acctgctgcc ttttcaatat agtggacaag
     6361 gttgtaggta aaagaatcgt agttatctat gactaaaatt ttcactcgtc aaaaatagcg
     6421 aatgttttat ggcacgacat tcaatcctga tcggtaaatt tatacccaac acctcttaca
     6481 gagataaaat acttagggtt tgacgggtcc ggttcaaaat attttctgaa tgtaacgata
     6541 aagttgtcta ttgttctagt gcttggataa atatcatagc cccaaacggt ttcaagaatg
     6601 tgttcgcgag aaacaacttc attgcgtttt tctatgagca atttcaacag taacgattct
     6661 ttttttgtaa gatgggtgat tgtaccgtca gcacgtttta tttcaagttg tgcaaaatta
     6721 atttgattgt cattcagatt ataggttgaa agcgatgccg catccttatt agctgtgctt
     6781 cttctgataa gattatgcac tcttagtaat agctcttcga ggttaaatgg tttagtcaga
     6841 taatcatcgc cacctagttt gagaccggta attctgtcgt taggatggtt tttggctgaa
     6901 agaaataaaa tgggcacatc aagattctca agacgtattg cttcgcaaac agtgaatccg
     6961 ctaatttcag gaagcataac atctaaaata accaaattat accgtgcttc tttaaatact
     7021 ttaatagcgg ttttgccatc gccaacagca ttcacatagt agccttccat ttcaaggtta
     7081 agctttattg tttcagacaa ggtttgctca tcttccacca acaaaattct tagattttga
     7141 tcagccatat caattaattt cgatgctaat taacaaaaat gttttctttg ttcattatgt
     7201 tgcgaaacta ttcactgtca caatcgggta aatattggat ttggtcaata ttttacagct
     7261 tattgtttct ggcagtttat ggttataaat tcaattgcag cgaccttgag gagcatttgc
     7321 ctgcagttta caaaatgctg aatccgcaat tatatcaagg cgattatgtg actgagtttc
     7381 tggcatccaa tttctcggtg cgctacttct ataatcatac attatattat cccggacttg
     7441 tgtttccatt gtcatcagtt tcatttacac tgatgctgat attgctgacg gtttttatat
     7501 tttacattca aaaaacatcg tttgtaattt ttaaaaatca cattacagct tccataactc
     7561 cattcattgc aatcattctg tttaataaaa ttacaattgg aggcaactct gtttcagaca
     7621 atatgctgac ctgtacagta tttgctaaca cactgtgtat gacagcatat tattacggat
     7681 tgagttcacg attgagaata gctgcacttt gttgtggact tgcaacttgg tatcaaccgc
     7741 ttgtaggttt gcaggtattt ttattgctgt tgttgatgca tttagtaata aataaattca
     7801 gagtcaaaga aatcggcact acaataattt tctacttttt gtcggcctct cctatgctga
     7861 tacctttatt caatcgtcag ttttttgcag aaagtgcagc acctgacaag cttttctatt
     7921 atgttttata tgttttcaga aacccacatc attatctacc ttcctttttc cccattgcag
     7981 attatattaa aagtggtata ttatttctga tatcaggctt gttatttttc agattcccga
     8041 caaaatacaa cagacaaata atggtaataa tttcaggcat tgttggcgga atgatttttt
     8101 actatttaat ttttgatgtc ggtggattaa tggttgttgg taaactacaa tggtttaaaa
     8161 cttcggtttg gcttactatt ttctgtagtt tttatatagc acatttcatt gcaaagcaaa
     8221 atttgataca aaatatctta aattataaat gggtatctgc ttgtgtaatt gctttatcaa
     8281 tttttatctt ttatttaatc acacatgcct cattattacc attagaaaaa ctaaaatcac
     8341 gctatgcagt tggtcagttt aaaaagactg atttaacact gatgcatgaa tggatcagag
     8401 aaaacacaac tgacactaca aagattttga gttttcctga tgatgattca tttttatgcg
     8461 aagcacaaag acctacacct gtggcatgga aagccattat acatgaacca tggtttctga
     8521 aaacatggta ttataatttc acaactgatt ataatctgtc aagaaaaatt gaatggaaaa
     8581 atatcaatca aagagaaaag gcaagtagta tttttttagg catacaagat aaaccaacta
     8641 atgcaagtta ctcaatgatt ttatttaatg ctaataaact accagaacaa cacaagggta
     8701 aaataattca ccggcaagga gaatataccc tatggcaatg gaactaaaaa atcaataaga
     8761 tgttttgagc tctactcttc tgtttttagc tcttccttca ggagtgtcgt ttccggcaat
     8821 tggcttagtt tcaccaaaac cggtagctgt taatcttgat tcaaaaatac ctttgctcat
     8881 cagatagctc ttaacggcat cacaacgttt ttgcgaaaga tttagattat aagcatcatc
     8941 acccttatta tcagtatgcc cttctattga cagatttgct gcatcatact ttttcaatat
     9001 tccaactaat gcatcaagct gaatgagtga agcaggcttg agtttatcgc tgttac
//
LOCUS       DAOOOL010000102         9015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_731_length_9015_cov_236.092746, whole genome shotgun sequence.
ACCESSION   DAOOOL010000102 DAOOOL010000000
VERSION     DAOOOL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_9015_cov_236.092746"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKU82_08695"
     CDS             <1..506
                     /locus_tag="PKU82_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ82989.1"
                     /translation="ASSHPVNMNFIYHERNMEFKFDNILLPDSNVNESLSHGFIHYRI
                     KPKTSLVLNDNIQNNAAIYFDFNDPVITNTATTHVVLPTGIASYQSPENIQLFPNPAQ
                     SEINLQHITAVEKIQVIDVLGKIVFEKNTNNQQYLKINISQVQNGVYFVKAYSAQGVV
                     VKKFVKR"
     gene            complement(611..2977)
                     /locus_tag="PKU82_08700"
     CDS             complement(611..2977)
                     /locus_tag="PKU82_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOZ82990.1"
                     /translation="MKSIITIVLLVLINANAFAQSEKYQKVRIWLEGKTVSSLAQTGV
                     DLSETHYRKGLYFETDLSTSELNSVSNAGFRTEVLIDDVKQFYKQRNEQQNQKTSAVM
                     SCSSAPDFPQPAHFGYGSMGGFFTYQELLNNLDSMASLYPNLITVKQPISNTLTTVEG
                     RPIFYVKISDNPNLTEPEPEVLYTALHHCREPNGMAAVIYYMWYLLENYATNTDIQQL
                     VNNTEMYFVPCVNPDGYVFNEMTDPNGGGMWRKNRQQFPGGEYGVDLNRNYGVQWGYD
                     DVGSSPDPADDTYRGPAAFSENETQMISAFCQQHNFKLALNYHTYSNLLIYPWGYIPD
                     YYTPDSALYKNYGDILTTYNKYAAGTANQTVGYMVNGSSDDWMYGEQTLKPKTFAMTP
                     EIGSADDGFWPQQSRIVDLCKENMYANVMLARLAGKYGQATDKSKWNIPALTSYIPFD
                     FKLLGLDTVGTFTVSLTPLSANIQTTGAAKTFSGLNFNLSVTDSITITLNSTTLPGDE
                     VKFLLTVNNGLYDISDTITKYYGPVVTPFFENGNTTSAWNTQFWGTATSSFISALSSI
                     ADSPIGDYQSSSINEITTISNINLLNAAAARLTFFTKWATEPQYDYAQLQISDNNGST
                     WTPLCGKYTVPGSPFQDVGDPVYQGVNLNWVKEDINLNAYTGKNVKLKFIMVSDGFVE
                     YDGIYVDDLSVEKIVASGVGINNLENNTFNIYPNPATDIARIETAANAKNINVINTLG
                     QTVFTENLILEQHHYDLNIADFAAGVYVVKVKYSSGAVAQKRLMVYKK"
     gene            complement(3079..4428)
                     /gene="tilS"
                     /locus_tag="PKU82_08705"
     CDS             complement(3079..4428)
                     /gene="tilS"
                     /locus_tag="PKU82_08705"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOZ82991.1"
                     /translation="MLLQFQQFVKEKKLFSKEQTILVAVSGGIDSMVLLQLLLQSGYK
                     IAVAHCNFKLRGEESDGDAQFVHSFCLENKIVIHSTEFETEKLAKQNQQSIQGTAREL
                     RYNFFQELCSSFHYDFIATAHHADDVAETVLINLSRGGGLAAWHGIEVKRENIVRPLL
                     FAQKHEIEEYAKQNKIQWRTDSSNQKEDYTRNLIRHQIIPVLKKINPSVVQTLFHHAE
                     IVADAEQIIHEHIEKFRAASVVTDENKTIVPISDLLNTSAAQTLLFYILQPYGFNATH
                     TVDIFKSLNHSESKTFYSDSFQLVKEREQLVIIPFEKKRISKIYFEIPTTGTFSIADS
                     QISVEVLENTEDLYHIILKNNNQVIGYFDADKIEFPIIVRTWQHGDYFFPLGMHGKKL
                     VSDFFTDEKMSQSEKQKQLLFLSSNEVIWLEGRRISEKFKVEKNSKSVLKISVKAIV"
     gene            complement(4457..5728)
                     /gene="pabB"
                     /locus_tag="PKU82_08710"
     CDS             complement(4457..5728)
                     /gene="pabB"
                     /locus_tag="PKU82_08710"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOZ82992.1"
                     /translation="MSDTKFSPAEFEELLKSSNGFRNFCFLNSNNAAEGSYSGLTKYD
                     FLAGINALDTVEATSESLEKLETFIQKNKGKWMFGFMSYELKNEIEQLKSSHTDRLNF
                     PVLHFFVPQVVLVSEGGKLRMIDQLEQTSKEFVADDVELRQMNASANVSLKPRFSNDD
                     YIQAVEKLKRQLQQGNIYEVTFCQEFYNDAANINPFETYIKLNTNTRAPMSCFYKKEN
                     QFIISSSPERFMCRRGNKIYSQPIKGTAPRSASREEDEWLKDNLQNSEKERAENLMIV
                     DLVRNDLSRIAKPGTVEVNELLSVYTFKTVHQLISTVSCEVEPKINFSEIIRAAFPMG
                     SMTGAPKISAMTLIDEHEKTNRGLFSGSVGYIDPSGDFDFNVIIRTILYNAQRNYVSV
                     QTGGAITIQSDPAAEYEECLLKAKAMMDVLG"
     gene            complement(5843..6928)
                     /locus_tag="PKU82_08715"
     CDS             complement(5843..6928)
                     /locus_tag="PKU82_08715"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOZ82993.1"
                     /translation="MNQIIQLLFPFISSLIIASLIIPSWIQLCSKWNLFDRPDTRKHH
                     TAIIPSLGGIGIFIAIMISIFLFGYNNQDSSFHSIAAAMLILFITGFFDDLTDLKARV
                     KLNIQLLAALIVTYSGLRIQTLNGFTGIHELPIIAQYGISIFIIIFLVNAYNFIDGLD
                     GLAATIGLIIFSIFTVLFHIHGQQAYAVLCVSVIGALVSFLYFNFNPARVFMGDTGSL
                     VVGFLIAICTLKLFNLWFTSPVVSFGPSLTIATIFILIFDLTRVVFIRVTNGISPFKA
                     DRSHLHHMICRQQFGHRGGTFIMAAFNILFIVLVLPLSKLRFITFLVFCFCVAILLMN
                     SKVMSYVALLRNKITGAPESKMGVDRG"
     gene            complement(6938..8428)
                     /locus_tag="PKU82_08720"
     CDS             complement(6938..8428)
                     /locus_tag="PKU82_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOZ82994.1"
                     /translation="MKKIIIIVTLYMLSGIVNGQDPHISQYFNVPVFYNPALSGNGIQ
                     NMRIAVDYRNQWLAPGLSNKTQTIALDKIVNKVGFGLMVSKNSAGKDGFSRLSVLGGI
                     SYHTLFGYKKRHQFSSGFQVGILQKSFDPNKLTFESQYTPDIGYDPSASNGETFANTK
                     VIRPDINVGIAYHFAQGNKEILIKPFAGVSFSHINKPKESFIEEQNVNPIRMNFHGGA
                     SITCSKTIELKPLVYFMKQDGFQEMSAGMISELSLSNNSLVKLGLLYRNNDAMVAYAG
                     YQFNKIFLGTSYDFNISKYKPGGSGAYEISLVYTPQGRAKGKRNEERKPEPVNKKKLP
                     EQRVTPPQVLTPIPVIAPSIIEQNPIVVSLAEMPMKGKSSMKEKTELSNYNYQTIGYV
                     VFQDNSLKYDINNRFDVIEATIDYLYKNTNRKIIVSGNASEIEIKKNNSIGKQRAETV
                     KDYMIGKGIDADRILIADVKDKMPALMIEDGIQVERLSRADILLIK"
     gene            complement(8438..>9015)
                     /locus_tag="PKU82_08725"
     CDS             complement(8438..>9015)
                     /locus_tag="PKU82_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOZ82995.1"
                     /translation="CTSISRFPVLAYIYPALHVDFTESQAVKADGGYAVSFQNNTPTG
                     NNTYTWNFGDGSANAISNEENPQHVYSSEGDYSVTLSVKDEYGCENSFTKNISVTVAH
                     DLFLPSAFTPNSDGINDIFRLKGSGFVSADMLILDQWGQLIFRTNNAVIGWDGSVKGN
                     LAPNGTYSYIVKIKLNDGNDKVLKGNISIIK"
BASE COUNT         2902 a         1696 c         1414 g         3003 t
ORIGIN      
        1 ttgcatcatc gcatcctgta aacatgaatt ttatatatca tgagcgcaac atggaattta
       61 agtttgataa tattcttttg cccgatagta atgttaacga gtcgttaagc catggcttta
      121 ttcattacag aataaaacct aaaacaagtt tggtattgaa tgataacata caaaacaatg
      181 ctgcaattta ttttgatttt aatgaccctg taattaccaa cacagcaaca acgcatgttg
      241 tactaccaac aggaatagca tcatatcaga gtccagaaaa catacaatta ttcccaaatc
      301 ctgcacagtc tgaaataaat cttcaacaca taacagccgt tgaaaaaata caagtgattg
      361 atgtacttgg aaaaattgtg tttgaaaaga ataccaacaa ccaacaatat ttaaagataa
      421 acatcagtca agttcaaaac ggagtttatt tcgtaaaggc atacagcgca caaggtgtgg
      481 tggtaaagaa gtttgttaag agataggcaa ttttagatta gccatcgctc agccgtggct
      541 cagccgcggc tgaggagatt tgttgaattt gtatctgcaa tgacttgttg gagtcacatc
      601 aattcatatt ctacttttta taaaccatca atcgtttttg tgcaacagca ccactgctat
      661 attttacttt aacaacataa acacctgcag caaaatcagc aatgtttaaa tcgtaatgat
      721 gttgttctaa aattaagttt tcagtaaata ctgtttgtcc caaagtattg ataacattaa
      781 tattttttgc attagctgca gtttcaattc ttgcaatatc tgttgcagga ttagggtaaa
      841 tattgaatgt gttgttttca agattgttta ttccaacacc tgaagctaca attttttcta
      901 cacttaaatc atctacataa ataccatcat attcaacaaa gccatcactt accataataa
      961 atttcaactt cacatttttt cctgtatagg cattcagatt aatatcttct tttacccaat
     1021 ttaaattcac accctgataa acaggatcac ctacatcctg aaaaggactt cccggtactg
     1081 tatattttcc acaaagtggt gtccatgtgc taccattgtt atcggaaatt tgcaactgtg
     1141 cataatcata ctgcggttct gttgcccact ttgtaaaaaa tgtcaaacgt gcagcagcag
     1201 cattcaacaa attaatatta ctgatagttg tgatttcatt aattgatgat gattgataat
     1261 cgccaatagg agagtctgct atgcttgata gtgccgagat aaaagacgat gtagctgttc
     1321 cccagaattg tgtattccat gctgatgtgg tatttccatt ttcgaaaaac ggtgtcacaa
     1381 cagggccata atattttgta atggtatcgg aaatatcata taagccattg tttacggtta
     1441 acaggaattt tacttcatca ccgggtaacg ttgttgaatt taatgtgatg gttattgaat
     1501 ccgtaaccga tagattaaaa tttaaaccac tgaatgtttt tgctgctcct gttgtttgga
     1561 tgttagcaga cagtggcgtt aacgaaacag taaatgttcc aacagtatct aatccaagta
     1621 atttaaaatc gaatggtata tatgaagtaa gtgcaggaat attccacttg cttttatcag
     1681 tagcctgacc gtatttacct gccagacgtg caagcataac attggcatac atattttctt
     1741 tgcacaaatc aacaatgcgt gattgttgcg gccagaagcc atcatctgca ctaccaatct
     1801 ctggtgtcat tgcaaatgtt tttggcttta atgtttgttc gccatacatc cagtcatcag
     1861 aacttccatt aaccatataa cctactgttt ggtttgcagt acctgctgca tatttattat
     1921 atgttgttaa aatatcacca tagtttttat acaaagcact gtcaggtgta taatagtcag
     1981 gaatatatcc ccatggataa atcaataaat tactatatgt atgataattt aatgccagct
     2041 taaaattatg ttgttggcaa aaagcactta tcatttgtgt ttcattctca ctgaaagccg
     2101 cagggcctcg ataagtatca tctgcagggt cgggcgatga accaacatca tcataacccc
     2161 actgaacacc ataattacga ttaagatcaa caccatattc tccacccgga aattgttgtc
     2221 tgttttttcg ccacataccc ccaccattgg gatcggtcat ttcattaaac acatatccat
     2281 ccgggttaac acagggcaca aaatacattt cagtattatt tactaactgc tgaatatcgg
     2341 tattggtagc atagttttcg agcagatacc acatgtaata tatcactgct gccataccat
     2401 taggctcgcg acaatgatgc aatgctgtgt aaagtacttc aggttcaggc tcggttaaat
     2461 tggggttatc agaaattttt acataaaaaa taggacgacc ttcaaccgtt gttaacgtat
     2521 tgcttattgg ttgttttaca gtgattaaat taggataaag cgatgccatt gaatcaagat
     2581 tgtttaacaa ctcctgataa gtaaaaaatc cgcccatgct accataacca aagtgtgcag
     2641 gttgcggaaa atcaggtgct gagctgcatg acattactgc tgatgttttc tgattttgct
     2701 gttcattacg ttgcttataa aattgtttta catcatcaat taatacttct gttctaaagc
     2761 cggcattgct gacactattt aattcagaag tacttaaatc tgtttcaaaa taaagtccct
     2821 tgcgataatg tgtctcacta agatctactc ctgtttgtgc aagtgatgaa acagtcttcc
     2881 cttcaagcca gattcgtact ttctgatatt tctctgactg agcaaatgcg ttagcattga
     2941 ttaacactaa caacacaatt gtaattattg atttcatttt aattgaataa gattttatat
     3001 tgcttttttg atatggcctt tacactaaca aaactatcta aaaaaatctt tactcaaagt
     3061 aattttaacc tgtttgtttt aaactatagc cttaacacta atttttaaaa ccgacttact
     3121 gtttttctcc actttaaatt tttcacttat acgtctgccc tcaagccata tcacttcatt
     3181 actacttaaa aacaacaatt gtttttgctt ttccgactga ctcatttttt catcagtaaa
     3241 aaaatctgat actaactttt tgccatgcat acctaatgga aaaaaataat cgccatgctg
     3301 ccatgtcctt acaataattg gaaattctat tttatctgca tcaaaataac cgattacttg
     3361 gttgttgttc tttaatatta tatgatacaa gtcctctgta ttctccaaaa cttctacact
     3421 tatttgagaa tcagcaatag aaaaggttcc tgttgttgga atttcaaaat agattttact
     3481 aatcctcttc ttttcaaatg gtattataac aagttgttct cgctctttta ccaattgaaa
     3541 actgtcagaa taaaaagtct tactttcaga atgatttaag gatttaaaaa tatcaactgt
     3601 atgtgttgca ttaaatccat acggctgaag aatataaaac aataaggttt gtgctgctga
     3661 tgtatttaac aaatctgaaa tcggaacaat agttttattt tcatcagtaa ctacagatgc
     3721 tgctctgaat ttttcaatat gctcatgaat aatttgttcg gcatctgcta ctatttctgc
     3781 atgatgaaac aatgtctgta caacagaagg atttatcttc tttaatacag gaataatctg
     3841 atgtctgatg agatttcttg tatagtcttc tttctgattt gagctgtcag tgcgccattg
     3901 aattttgttc tgtttagcgt attcttcaat ctcatgtttt tgtgcaaaca ataaaggcct
     3961 gacaatattt tctctcttta cttcaatacc atgccatgca gccaaaccac caccccgact
     4021 caaattaatt aatacagttt ctgcaacatc atcggcatga tgagccgtag caataaaatc
     4081 ataatgaaag ctactacata actcctgaaa aaaattatat ctaagttcac gtgctgtacc
     4141 ctgaatggat tgttgatttt gttttgccaa cttttctgtt tcaaattcag tagaatgaat
     4201 cacaattttg ttttccaaac aaaatgaatg aacaaactgt gcatcaccat cagattcttc
     4261 accacgcaat ttaaaattac aatgtgcaac agcaatttta tatcctgact gtagcagtag
     4321 ttgcaataaa accattgaat caatgcctcc actaacagct acaagaattg tttgctcttt
     4381 actgaagagt tttttctctt ttacaaattg ttgaaactgt aataacaagt gttgctttat
     4441 tttctgctaa tgtacatcat cctaaaacat ccatcattgc tttagctttt aacaaacact
     4501 cttcatactc ggcagcaggg tcagattgaa tggttattgc tccgcccgtc tgcactgaaa
     4561 catagttacg ttgtgcatta tacaatatag tacggataat aacattgaag tcaaaatcgc
     4621 ctgaaggatc aatataacca acactcccgg aaaacaagcc tctgtttgtt ttctcatgtt
     4681 catcaatcaa tgtcatagcg cttattttag gagcacctgt catacttccc ataggaaatg
     4741 cagcacgtat gatttcagaa aaattaattt tgggttcaac ctcacagctt actgtagaaa
     4801 tcaattgatg tactgtttta aacgtataga cactaagcaa ctcattcact tcaactgtgc
     4861 cgggcttagc aattcttgaa aggtcatttc gtaccaaatc aacaatcatc aaattttctg
     4921 cacgttcttt ttcactattc tgcagattat ctttaagcca ttcatcttcc tcacgtgatg
     4981 cgctgcgtgg tgctgttccc tttattggct gcgaataaat tttatttccc cttctacaca
     5041 taaaccgctc aggacttgat gaaataataa attgattttc ttttttataa aaacacgaca
     5101 tgggagcacg tgtgtttgta ttaagtttta tataggtttc aaacggattg atgtttgctg
     5161 catcattata aaactcctga caaaaagtta cttcataaat atttccctgc tgtaactgtc
     5221 tctttaattt ttcaacagct tgtatgtaat catcattgct gaaacgaggc tttaaagaaa
     5281 cattagcaga tgcattcatt tgtctaagct ctacatcatc ggcaacaaat tctttcgatg
     5341 tctgttcaag ctgatcaatc atacgaagtt ttccaccttc agaaactaaa acaacttgtg
     5401 gtacaaaaaa atgtaaaaca ggaaaattca atcggtctgt atgcgatgat tttagttgtt
     5461 cgatttcatt ttttagctca tacgacataa aaccaaacat ccacttgcct ttattcttct
     5521 gaataaatgt ctccagcttc tctaaagatt ctgatgttgc ctcaactgta tcgagcgcat
     5581 taatgcctgc aagaaaatca tattttgtca agccactata gctaccttct gcagcattgt
     5641 tgctgtttaa aaaacaaaag ttacgaaaac catttgatga ctttaacagc tcttcaaatt
     5701 cggcaggtga aaatttagtg tcgctcataa agcaaaaaaa atcaaaaaag atttctgttg
     5761 tttatgattc taaaaaagta gtgaacacgt ttttaacaag ttcaataaat tgaaatgttt
     5821 tataagtatt attcaccgat aattaccctc tgtcaacacc cattttactc tcgggtgctc
     5881 cggtaatttt atttcttaat agtgcaacat acgacatgac cttagagttc ataagtagaa
     5941 tagctacaca gaaacaaaaa acaaggaatg tgataaaccg cagtttactt aacggaagaa
     6001 ccaaaacaat aaataaaatg ttgaatgcag ccataataaa agtgcctcca cgatgtccga
     6061 attgctgacg gcaaatcatg tgatgtaaat ggcttctgtc tgctttaaac ggtgaaatac
     6121 catttgttac cctgataaaa acaactcttg ttaaatcaaa tattagtata aaaattgttg
     6181 caatggttaa cgaagggcca aaagaaacta cgggagatgt aaaccaaaga ttaaaaagtt
     6241 ttaaagtgca aatggcaata agaaaaccta caactaacga acccgtatcg cccataaata
     6301 ctcttgcagg attaaaatta aaatacagaa aagataccaa agcaccaata acactgacac
     6361 ataaaactgc ataagcctgt tgtccatgaa tatgaaacaa tacagtaaaa atgctgaata
     6421 taataagccc tattgttgct gccaagccat ctaatccatc aataaaatta taggcgttta
     6481 ctaaaaatat tataataaaa atactaatgc catattgagc tatgataggt aattcatgaa
     6541 tgcctgtaaa accatttaat gtttgaatgc gtaatcctga ataggtgact attaaagctg
     6601 ccaagagttg tatatttaat tttactcttg cttttaaatc cgtaagatca tcaaaaaatc
     6661 cggttataaa taaaatgagc attgccgctg caatggaatg aaaacttgaa tcctgattgt
     6721 tgtatccaaa caaaaagatc gaaatcatta tagcaataaa aattccgata cctcctaacg
     6781 aagggataat tgctgtatga tgtttgcgag tgtcggggcg gtcaaacaaa ttccatttag
     6841 aacaaagctg aatccacgat ggaatgatga gtgatgcaat aataagagaa gaaataaatg
     6901 gaaataacag ttgaataatc tgattcatac aggtggttta tttaatcaat aatatatctg
     6961 ctcgcgatag tctttctact tggattccgt cttctatcat taatgcaggc attttgtctt
     7021 taacatcagc aatcaggatt ctgtcagcat caattccttt acctatcata taatctttaa
     7081 ccgtttctgc tctttgtttt cctatactgt tatttttctt tatttcaatt tccgaagcat
     7141 ttcctgaaac aataattttt ctgtttgtgt ttttatacaa ataatcaatt gtagcttcta
     7201 ttacatcaaa gcggttattg atatcatact tcaaactatt atcctgaaac actacatacc
     7261 ctattgtttg atagttgtaa ttagaaagtt ctgttttctc tttcatactg cttttacctt
     7321 tcattggcat ttctgcaagt gaaacaacta taggattctg ttcaattatt gaaggagcaa
     7381 ttacaggtat aggtgtcagc acttgtggag gagtaactct ttgctcaggc aattttttct
     7441 tattgacagg ctcaggtttt ctttcttcat tacgtttacc ttttgcacga ccttgtggcg
     7501 tgtaaacgag tgaaatttca taagcgccac tacctcccgg cttgtacttc gaaatattaa
     7561 agtcatagct tgttccaaga aatattttat taaactggta gcccgcataa gcaaccatag
     7621 catcgttatt tctgtaaagc aatcctaatt ttactaaact gttattgctt aatgacaatt
     7681 cagaaatcat accagcactc atttcctgaa agccatcttg cttcataaaa taaacaagag
     7741 gtttcagctc aatagttttt gaacaagtaa ttgatgcacc tccatgaaaa ttcattctta
     7801 taggatttac attctgttct tcaataaatg attctttagg tttattgata tgagaaaatg
     7861 aaacaccggc aaatggttta atcagaattt ctttattgcc ttgtgcaaaa tgataggcta
     7921 tacctacatt aatatcaggg cgtataactt ttgtgttcgc aaatgtttct ccatttgatg
     7981 ctgaaggatc atatccaata tcaggagtgt actgactttc aaatgtaagt ttgttggggt
     8041 cgaaagattt ttgaagaatt ccaacctgaa atcctgacga aaactgatgt ctttttttat
     8101 agccaaatag tgtatgataa cttatgcctc ccagaacact taatctgcta aagccatctt
     8161 tacctgcact gtttttacta accattaaac caaaacccac cttgttgaca attttatcta
     8221 atgctattgt ttgtgttttg ttactcaatc cgggagcaag ccactgattg cgataatcaa
     8281 ctgcaatgcg catattctga ataccattac ccgataaagc aggattgtaa aatacaggta
     8341 cattaaaata ttgtgatatg tgaggatcct gtccattaac aattcccgac aacatataaa
     8401 gtgtaactat gataattatt tttttcatgt ctgccggtta tttgatgatt gaaatattgc
     8461 ccttcaacac tttatcattg ccatcattca gctttatctt tactatgtaa ctatatgttc
     8521 cattgggtgc aagatttccc tttacagagc catcccatcc tattactgca ttgtttgttc
     8581 tgaaaattaa ctgtccccat tgatcaagta tcaacatgtc agctgaaaca aatccagaac
     8641 cttttagtct gaaaatatca ttgatgccat cgctattagg tgtaaaagca gagggtaaaa
     8701 acaagtcatg tgcaacggta acagaaatat tttttgtgaa actgttctca catccatatt
     8761 catccttaac tgatagtgtt acactgtaat ccccttcaga tgagtaaaca tgttgtgggt
     8821 tttcttcatt agaaatcgca ttggcagaac catcaccaaa attccaggta taggtgttat
     8881 tccctgttgg tgtattgttt tgaaatgata cagcataacc accatctgct ttcacagcct
     8941 ggctttctgt aaaatcaaca tgtaaagcag gatagatgta tgcaagtaca ggaaatcttg
     9001 aaatgcttgt gcagc
//
LOCUS       DAOOOL010000103         8902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_752_length_8902_cov_77.321013, whole genome shotgun sequence.
ACCESSION   DAOOOL010000103 DAOOOL010000000
VERSION     DAOOOL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_752_length_8902_cov_77.321013"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKU82_08730"
     CDS             <1..368
                     /locus_tag="PKU82_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOZ82996.1"
                     /translation="VALADARKAIAMFQLPSVNIPVFGLIENMSWFTPEELPDNKYYL
                     FGKDGGQKLAAEMNVKLLGQVPLVQSVREGGDIGNPIATGNSIVSEAFNELSKSVAQQ
                     VAIRNAVIEPAQRVPAAGF"
     gene            380..640
                     /locus_tag="PKU82_08735"
     CDS             380..640
                     /locus_tag="PKU82_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOZ82997.1"
                     /translation="MSESLKNNLKHKVEQALDTIRPYLKSDNGDVTVLDITPQNVVKL
                     RFEGACSSCTMSAMTFKAGVEETILRMVPEITAVEALNVENV"
     gene            706..1425
                     /locus_tag="PKU82_08740"
     CDS             706..1425
                     /locus_tag="PKU82_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ82998.1"
                     /translation="MKITAAVVVSILFFTGTIHAQTFVVFKGDTINRKDAKGLKQGIW
                     KKFYRTDSLFSITSFKDNKQTGTTKFYYESGKLKSTLIWKKPGQLAFAESFFEEGKKM
                     SDGYYLNEQKDSTWHYYNEEGKITSQETYRKGVKQGKWLTYYPEGGKVEEKNYMSGKL
                     EGPYLKFFKDGKLMLKATYKNDKLTGGYTIYNADGGFYTTGQYNAGDRAGKWTVFNEK
                     GKPWIESQYKNGFEVKTDTLQ"
     gene            1447..2553
                     /gene="mnmA"
                     /locus_tag="PKU82_08745"
     CDS             1447..2553
                     /gene="mnmA"
                     /locus_tag="PKU82_08745"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOZ82999.1"
                     /translation="MSKKGRILVAMSGGIDSSVTAMMLHDEGYEVIGITMKTWDYATS
                     GTSGKETGCCSLDSINDARSIAVEKGFPHYILDIRGEFGDFVIDNFVDEYLAGRTPNP
                     CVLCNTHIKWEALIKRADMLDCEYIATGHYASVRNEGGRYIVSRGKDLNKDQSYVLWG
                     LSQKNLARTRFPLGNYKKSEIRNMAFDMGFKELANKSESYEICFVPDNDYRAFLKSRV
                     EGIEEKLDGGVFVNTKNEVIGKHKGYPFYTIGQRKGLEVAFGKPMYVTEIKPNTNTVV
                     LGELEDLQKTTMKVRGLNLVKYESLSNPTEVLTKIRYKDPGTMSVITQTSAVNAAVEF
                     HAPVTAVAPGQSAVFYEGNDVVGGGFIDKEYGKV"
     gene            2540..3211
                     /locus_tag="PKU82_08750"
     CDS             2540..3211
                     /locus_tag="PKU82_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83000.1"
                     /translation="MAKSKNIFSLIILALVLLMGCTKDKETVSAIDYRYYPIAIGDTM
                     IYQVSLFNKDLNTYDSTYQLLERVESVFNDNEGRPTYRLERYVRNTDTDAWSIYKVWT
                     TNKTTTSIEKQEDNITYIKLIFPVKLNKTWNGNAKNIFADDFDDYKIISLNEPLQSGN
                     YSFDSTLNVQQIDYDYGFEKRKHFEIYATGVGMIYKDQYDSLQGSPLGKIYHYTETLL
                     YHNKK"
     gene            3214..4884
                     /locus_tag="PKU82_08755"
     CDS             3214..4884
                     /locus_tag="PKU82_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOZ83001.1"
                     /translation="MKKIYLLILIFLTSHELYSQNRYAVMLTDKNNSSFSFSNPSAFL
                     SQRAINRRLQFGIAIDSLDLPVNATYLTAIQNTGAVILNTSRWLNEVTVDVSVNPNAI
                     AAINALPFVKQTKLASRTTNRTNSKFSFEIESLMERQSQTQKVASTASFYNYGNALNQ
                     IQMLHGDNLHDLGFRGDGKIIAMLDAGFLRADSMTAFDSLRAHNRILSTYDFVDHNSN
                     VYDDHTHGSMCFSIIGANDPGNIVGTAPEASFLLYRSEDAATEKLIEEYNWATAAETA
                     DSAGTDIISSSLGYSVFEDSTMNHTYNDLDGRTAPISIAANIASRKGIVVVSAAGNEG
                     NGQWHYITAPADADSILTIGAVDAAGVYASFSGTGPSFDGRIKPSVVAQGQGTFVSDP
                     YSNTVFSGNGTSFSCPVIAGLVASLWQAIPSANNMQIINAIVQSASQYNNPDSLLGYG
                     IPNFDSARNSLLAVFNPYYGKSDFIEKIFPNPFKNQLNINFYADSNSQYTSEIFDLSG
                     KQVMMKRGKFVPYSINSINLATTAMAKGVYLLRITTEKNVFQSRILKQ"
     gene            4891..5907
                     /locus_tag="PKU82_08760"
     CDS             4891..5907
                     /locus_tag="PKU82_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOZ83002.1"
                     /translation="MSSIGKKLKIIGTNFAFLILFLSLAEVGLRIANLGYDNAAFEPD
                     SVLHHIHRPDYSFIHSNAAEHEYGNIPVRYDKIGCVVNPVKILNVQCNKRVWLFGDSY
                     IEALQVNYDSSVIGILEKDFPDVCFKNFAVGAYSPVLHYLKLKQLLKNDKPDVVLLFL
                     YANDAAEDKQYLNEAKLAGGEVTAVDGGMPQWKYEVIRNSNLLRLIRKFYMQMTVKLE
                     NKKYMNESALSENNAILAEPTLTYLEKFVTLCKKNNVRVIVSCIPSKTDVLRNDFNSA
                     SFSSQIKAWCTGRCDFIDLQEPLQRFYLQTNTSPYYKNDIHFNNYGHRIVAMTIAQLQ
                     TIKN"
     gene            complement(5887..6354)
                     /locus_tag="PKU82_08765"
     CDS             complement(5887..6354)
                     /locus_tag="PKU82_08765"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOZ83003.1"
                     /translation="MSLNINHPAPLFESFNSEKQPFSLEQLKGKNVLLLFFPAAFTST
                     CTKELCSVRDNLSYYNNLNCTVIGISTDTIYSLAKYKEEQKLNFQLVSDFNKEIGKIY
                     HAQYELFGLGMKGITKRAAFLIDKTGILRYIEVLENASEIPDFNKIKSVLDSL"
     gene            complement(6360..7511)
                     /locus_tag="PKU82_08770"
     CDS             complement(6360..7511)
                     /locus_tag="PKU82_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83004.1"
                     /translation="MQYFSLILIISFLFSWFQSTAQHSVLFGERNLNDTILSVYFDRN
                     GKPYPDYFIADSALNINTGSLDTFYLNNPKEFAAICSLYKCRFSQYSISNLSILNDSI
                     FQTNADKINNQTNEGQVPTFMIHGFRKSYVNKPGVFTSVIDYALLMESLNRLSTEKYS
                     YIRVFWDGSYLDISEGVKQMKQIFTVFEKAQFNAISVGYGLRQILSRTTSPEVNIISH
                     SLGAVVVTNAMFDTTVNNYPTPNNSNINICLIAPAISGIEAFKSYYSRKSTYPFKKSD
                     NYSLFVLYNKKDFVLRKKDYFLELFGPGPRKHGNTTLGCNSNNEAIKLKDYFIQNFPN
                     SKITLINMTDLINKCHHLRCYCEGDNLKTVIQLMNHTNINSNGNKILNK"
     gene            7647..8687
                     /locus_tag="PKU82_08775"
     CDS             7647..8687
                     /locus_tag="PKU82_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83005.1"
                     /translation="MSFQRPGLVALTTGVACGSVFLFSEYLYGISTDNLLFIIVGSIA
                     VAFFVFLLINYFVLASFRSKLINIYRSILDKKNVPYTSVHHAKNIDKVYDDVLNEIKN
                     WESEKQTEIDELKKAENYRREFLGNVSHELKTPLFSAQGYVHTLIDGGIEDSDVNMLY
                     LKKASKSLDRLIAMVEDLESISELETGVVPLEIITFDIVELIKEVNESLEIKANERKI
                     RLIVESETERVYVYADKDKIRQVLVNLIENSIKYGREMGTTKVVVFTHSDSVMIEVAD
                     DGIGISSNNLPRLFERFYRVDKSRSREVGGTGLGLAIVKHIIEAHHQTIKVSSEKDKG
                     TVFSFSLSVSKK"
BASE COUNT         2980 a         1366 c         1742 g         2814 t
ORIGIN      
        1 atgtagcatt agccgatgcc agaaaagcaa tagcaatgtt tcagcttcca tcagtaaata
       61 ttcctgtttt tggattaata gaaaatatgt cgtggttcac tcctgaagag cttcctgaca
      121 ataaatatta tctttttgga aaggatggtg gacaaaaatt agctgcagaa atgaatgtaa
      181 aattactcgg tcaggttcca ttagttcagt ctgttcgcga aggtggcgat ataggaaatc
      241 caattgcaac aggaaattca atagtttcag aagcatttaa cgaattatca aaatctgttg
      301 cacaacaggt agcaattcgt aatgctgtta ttgagccggc acagcgtgtt cctgctgcag
      361 gattttaaaa agtgtaagaa tgagtgagtc attaaaaaat aatttaaaac ataaggtcga
      421 gcaggcattg gataccattc ggccctattt gaaatccgat aatggtgatg ttaccgttct
      481 tgatattaca ccacaaaatg ttgttaagtt gaggtttgaa ggagcatgca gttcgtgtac
      541 aatgagtgca atgacattta aagcaggagt tgaagaaact attttacgga tggtgcctga
      601 aattactgct gtagaagcat tgaatgtgga aaatgtttaa atggtaattt tatctaaaat
      661 aaaacagact tgttgatgtt atctcttaat aagcgttttc ttccaatgaa aattacagct
      721 gctgttgttg tttctatttt attttttacc ggcactatac atgcacaaac ttttgttgtt
      781 ttcaaaggcg atactataaa tcgtaaagac gctaaaggac ttaaacaagg tatatggaaa
      841 aagttttaca gaacagacag cttgttttcg ataacatcat ttaaagacaa caaacaaact
      901 ggtacaacaa aattttatta cgaatcaggt aaattaaagt caacactcat ttggaaaaaa
      961 cccggtcagc ttgcttttgc agaaagcttt ttcgaagaag gaaaaaaaat gtctgatggt
     1021 tattatctga atgaacagaa ggacagcact tggcattatt ataacgaaga gggtaaaatt
     1081 acatcacagg aaacctatcg caaaggtgta aaacaaggga aatggcttac ctattatcct
     1141 gaaggtggaa aagtagagga gaaaaattat atgagtggta aattggaagg gccatactta
     1201 aaatttttta aagatggaaa attaatgcta aaggcaacct ataaaaacga taaacttaca
     1261 ggtggttata ccatctataa tgcagatggt ggattttata caacagggca atataatgcg
     1321 ggagacaggg caggtaagtg gactgttttt aatgaaaaag gcaagccgtg gattgagagt
     1381 caatataaaa atggatttga agttaagaca gatacacttc agtaataatt aaagtaactt
     1441 tgcaaggtga gtaagaaagg tagaatttta gttgccatga gtggtggtat tgatagctct
     1501 gttacagcta tgatgctgca tgatgaaggt tatgaagtta taggcatcac aatgaaaaca
     1561 tgggattatg caacatcggg aacgtccggt aaagagacag gatgttgcag cctcgactca
     1621 ataaacgatg ccagaagtat tgctgtagaa aaaggatttc cgcattatat actcgatatc
     1681 agaggtgagt ttggcgattt tgtgattgat aattttgttg atgaatacct tgccggacga
     1741 acacccaatc cctgtgtgct ttgtaataca catatcaaat gggaagcatt aattaaacgt
     1801 gccgatatgt tagattgtga gtatatcgct accggacatt atgcatctgt acgcaatgaa
     1861 ggaggtcgtt acattgtcag cagaggaaaa gatttaaata aagatcaaag ctatgtgttg
     1921 tggggtttaa gtcagaaaaa tttggcgcga acacgatttc cattagggaa ttataaaaaa
     1981 tcagaaattc gcaatatggc attcgacatg ggatttaaag aattagccaa taaaagtgag
     2041 agctatgaga tttgttttgt tccggataat gactacagag catttttaaa atcaagagtt
     2101 gaaggtattg aagaaaaact tgatggtggc gtatttgtga atacaaaaaa tgaagtaata
     2161 ggaaagcata aaggatatcc tttctataca attggtcagc gtaaaggtct tgaagttgca
     2221 tttggtaagc caatgtatgt tacggaaata aaaccaaaca caaatactgt ggtattgggt
     2281 gagctggaag atttacagaa aactactatg aaggtacgtg gattaaatct tgtaaagtat
     2341 gaatcgctca gtaatcccac agaagtactt acaaaaatac ggtataaaga cccgggcact
     2401 atgagtgtca taacgcaaac atctgcagtc aatgctgctg ttgaatttca tgcaccggtt
     2461 actgctgttg ctcccggaca gagtgctgtt ttctacgaag gcaacgatgt agtaggagga
     2521 ggatttattg ataaagagta tggcaaagtc taaaaacatt ttctcactga tcatcctggc
     2581 tttagtatta ttgatggggt gtactaaaga caaggaaaca gttagtgcca ttgactatcg
     2641 ttattatcca atagcaattg gcgatactat gatctatcag gttagtttgt ttaataaaga
     2701 ccttaacacc tatgatagta cctatcagtt attagaaaga gtagaatctg tttttaatga
     2761 taacgaaggt cggccaacat accgtttaga acgttatgtt cgtaataccg atacagatgc
     2821 atggagtatt tataaggtgt ggacaaccaa taagactact accagtatag agaaacaaga
     2881 agacaacatt acttatataa aactcatttt ccctgtaaaa ctcaataaaa catggaatgg
     2941 taatgcaaaa aatatttttg ctgatgactt tgatgattat aaaattattt ctctgaatga
     3001 accattgcaa tcaggaaatt attcatttga cagtacttta aatgtgcaac aaatagatta
     3061 tgattatggc tttgaaaaaa gaaagcattt tgaaatttat gccaccggag taggaatgat
     3121 ttacaaagac cagtatgatt ctttgcaagg aagtccgctt ggtaaaattt atcattacac
     3181 agagacatta ctttatcaca ataaaaaata agcatgaaaa aaatttactt gttgattctg
     3241 atttttctga catcacatga gttgtatagt cagaatcgtt atgcagtaat gctgacagat
     3301 aaaaataact cttcttttag tttctctaat ccatctgcct ttttatctca aagagcaatt
     3361 aacagaagat tgcaatttgg tattgctata gattcactcg atttaccggt taatgcaact
     3421 tatcttacag caattcaaaa taccggagca gtaattttaa atacatcacg ctggctaaat
     3481 gaagtgacag tagatgtatc cgtaaatcca aatgcaatag cagcaataaa tgcattgcct
     3541 tttgtgaaac agacaaaatt ggcttcacgc acaactaaca gaacaaacag caagttcagt
     3601 tttgagatag aaagtttaat ggagcgtcaa tctcagacac agaaagtagc ttcgactgca
     3661 tcattttata attatggaaa tgcactcaat caaattcaaa tgctgcatgg cgacaactta
     3721 catgatttag gattcagagg tgatggaaaa attattgcta tgcttgatgc cggatttctt
     3781 agagctgaca gtatgactgc attcgacagt ctgcgtgcac ataacagaat tttgtccact
     3841 tatgattttg ttgatcataa cagtaatgtt tatgacgacc acacacatgg ttccatgtgt
     3901 ttttcaataa ttggcgctaa tgatcccgga aatattgttg gtactgcacc tgaagcttca
     3961 tttctattgt atcgttcaga ggatgcagca acagaaaagc tgatagaaga atataactgg
     4021 gcaacagcag cagaaacagc agacagtgca ggaacagata ttatctcaag ttcattagga
     4081 tatagtgtgt ttgaggattc tacaatgaat catacttaca atgatttgga tggcagaaca
     4141 gcacccatat ccattgcagc caacattgct tcacgaaaag gaattgttgt tgttagcgct
     4201 gccggtaatg aaggtaatgg tcaatggcat tacataacag cacctgcaga tgccgatagt
     4261 attttaacaa ttggcgctgt tgatgcagca ggagtatatg cttcattcag tggcacaggt
     4321 ccatcttttg atggaagaat aaaaccatct gtagttgcac aaggacaagg cacatttgtt
     4381 tcggaccctt attcaaatac tgttttctcc ggtaatggaa cttctttttc atgcccggta
     4441 attgcaggat tagtggcttc attgtggcaa gcaattccat ctgcaaacaa catgcaaatt
     4501 attaatgcca tagtgcagag tgcatcacag tataacaatc ctgactcatt gctgggttat
     4561 ggcattccta attttgattc tgcacgtaac tctttacttg cagtttttaa tccatattat
     4621 ggtaaaagcg attttatcga aaaaatattt cctaatccat ttaagaacca actgaacata
     4681 aatttttatg cagatagtaa cagtcaatat acttcagaaa tatttgatct cagtggaaag
     4741 caagtaatga tgaaacgtgg caaatttgtt ccatattcta tcaacagcat aaaccttgct
     4801 accacagcca tggcaaaagg agtttatttg ttgcgtatca ctactgaaaa gaatgttttt
     4861 cagtcgcgaa ttttgaaaca ataggcttca atgtcatcaa tagggaagaa gcttaaaatt
     4921 ataggaacca acttcgcttt tttaatattg tttttatccc ttgcggaggt tggattacga
     4981 attgcaaatc tcggttatga caatgcagcc ttcgaacccg attctgttct gcatcatatt
     5041 cacagacctg attattcgtt tattcattca aacgctgcag agcatgagta cggtaatatt
     5101 cctgtcaggt atgataagat aggttgtgtt gttaaccctg ttaaaattct gaatgtgcag
     5161 tgtaataaaa gagtttggtt atttggcgac tcttatattg aagcattgca agtaaattat
     5221 gattcatctg ttataggtat tttagaaaaa gattttccgg atgtatgttt taaaaatttt
     5281 gctgttggtg catacagtcc agttctgcat tatttaaaat taaaacaatt gttgaagaat
     5341 gataaacctg atgttgtcct tttgttttta tatgccaatg atgctgcaga agataaacag
     5401 tatcttaatg aagccaagtt agctggagga gaagtaacgg ctgttgatgg tggtatgcca
     5461 caatggaaat atgaagttat taggaactca aacctgcttc gtttgatacg taaattttat
     5521 atgcagatga ctgtgaaact ggaaaataaa aagtacatga atgagtctgc tttgagtgag
     5581 aataatgcaa ttcttgcaga gccaacacta acttatttag aaaaatttgt tacactttgt
     5641 aagaaaaata atgttagagt tatagtgtcg tgcatacctt caaagacaga tgttctgaga
     5701 aatgatttta attctgctag tttttcaagt cagataaaag catggtgtac cggaagatgt
     5761 gattttatag atttacaaga gcccttacag agattctatt tgcaaactaa cacttcacca
     5821 tattacaaaa atgatattca ttttaataac tacggccacc gcattgtggc aatgacaata
     5881 gcgcaattac aaactatcaa gaactgattt gattttatta aagtcaggaa tttcacttgc
     5941 attttctaat acttctatgt aacgtagaat cccggtttta tcaatgagaa atgcagcacg
     6001 ttttgtaata cctttcatcc ctaatccaaa caactcatat tgtgcatgat aaattttacc
     6061 tatttctttg ttgaaatcgg agactaactg aaaattcaac ttctgctctt ctttatattt
     6121 tgctaacgaa taaatagtat cggttgagat gccaattact gtgcagttta agttattata
     6181 ataagacaga ttgtctctga cactacacaa ttcttttgta caagtactgg taaatgctgc
     6241 aggaaaaaat aggagtaaaa cattcttgcc ttttaattgc tcaagtgaaa acggttgctt
     6301 ttctgaattg aaactttcaa aaagtggtgc agggtgatta atatttagtg acataatatt
     6361 tatttgttta atattttatt tccgtttgaa ttaatatttg tatggttcat caattgtata
     6421 actgttttta agttatcgcc ttcgcaatag caacgtaaat ggtgacattt attaattaag
     6481 tctgtcatat ttatgagagt aatttttgaa ttagggaaat tttgaataaa ataatctttt
     6541 agtttaattg cttcattgtt tgaattacat cccaaagtgg tgttaccatg ctttcttggc
     6601 cccggaccaa acaactctaa gaaatagtct ttcttgcgca acacaaaatc ttttttattg
     6661 tataatacaa atagactata gttgtcactc tttttaaatg ggtatgtgct ttttcttgaa
     6721 tagtaacttt taaatgcttc tattccgctt attgcaggtg caattagaca tatattaata
     6781 ttgctgttat tgggagtagg atagttgttt actgttgtat caaacatagc attggtcact
     6841 actacagcac caagactgtg tgatataatg ttgacttccg gtgaagtggt tcgtgaaagt
     6901 atttgcctta atccatagcc aacagaaata gcattaaatt gcgctttctc aaagactgta
     6961 aaaatttgtt tcatctgctt tacgccttcc gatatatcca gataactacc atcccaaaac
     7021 acccttatat aggaatactt ttcagtgctt agacgattta acgactccat taataaagca
     7081 tagtctatca cagaagtaaa aacgccaggt ttgttaacat aagattttct gaaaccatga
     7141 atcatgaagg tcgggacctg accttcattt gtttgattat ttattttatc agcattggtt
     7201 tgaaaaattg aatcatttaa aatgcttaaa tttgaaatac tatactgact aaaacgacat
     7261 ttatataaag aacatattgc ggcaaattct ttcggattat ttaagtaaaa agtgtctaat
     7321 gatccggtat taatattcag tgcagagtca gcaataaaat agtccgggta tggctttccg
     7381 tttctgtcaa aatagactga tagaattgtg tcattcaggt ttctttctcc aaacaagaca
     7441 gaatgctgtg ccgtactttg aaaccaggaa aacagaaatg aaattattag tatcagtgaa
     7501 aagtactgca taaatattag aacagtttaa aaacgcattt atccattgat tgtttacaag
     7561 aatgatattt aaaaaggcaa aaatatcttt taaattttta ccaatattgt tctgagtgat
     7621 ttgttgaagt tggctaattt tgtgaaatgt catttcaaag accggggtta gttgcattaa
     7681 caacaggagt tgcctgtggt tctgtttttt tatttagtga atatttatat ggaattagta
     7741 ctgataattt actttttata attgttggta gtattgccgt tgctttcttc gtttttctcc
     7801 tcataaacta ttttgtttta gcatcattca ggagcaaact aataaacatt tacagaagca
     7861 ttctcgataa gaaaaacgtg ccatatacct cagtgcatca tgctaagaat attgacaagg
     7921 tttatgatga tgtgcttaat gagatcaaga attgggagag tgagaagcaa acagaaattg
     7981 atgaattaaa gaaagcagaa aactacagac gtgaatttct tggtaatgta tcacatgaac
     8041 ttaagactcc actcttcagt gctcaggggt atgttcatac tttgattgat ggaggcattg
     8101 aagattcaga tgtaaatatg ctttatctga aaaaagcatc caagagcctc gacagactaa
     8161 ttgccatggt tgaggatctc gagtcaatat cagaattgga gacaggtgtt gtccctctag
     8221 aaattataac ttttgatatt gttgaactga ttaaggaggt taatgagtca ttagaaatta
     8281 aagcaaacga acgtaaaatc aggttgattg ttgagtcgga aacagaacgt gtttatgtct
     8341 atgccgataa agacaaaatt cgtcaggtgc ttgtgaatct gatagaaaac tcgattaaat
     8401 atggtagaga aatgggtact acaaaagttg ttgtatttac acacagtgat tcggtgatga
     8461 tagaagttgc agatgatggt attggtattt cttccaataa tcttccacga ttattcgagc
     8521 gattttacag agttgacaaa agcaggtcac gagaagtagg agggactggg cttggtttag
     8581 ctattgtgaa acatattatt gaggcacatc atcaaactat taaagttagt agtgaaaaag
     8641 ataaaggcac cgttttttca ttcagcctgt cggtaagtaa gaaataagcc ttttattgtt
     8701 gttgttcgaa gtcattcctt tgtagaagac attgtgagat tgtgaataga taacgtaaac
     8761 agtgaacata aataaaaaag tccgcatttg cggactttgt gggagcaaca ggattcgtca
     8821 gccgcggctg actgtaccct ctgctagaga atgatcttgg atttgagaaa gtctctaccg
     8881 gcaccggttt taaaatattt tt
//
LOCUS       DAOOOL010000104         8701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_774_length_8701_cov_92.816331, whole genome shotgun sequence.
ACCESSION   DAOOOL010000104 DAOOOL010000000
VERSION     DAOOOL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_8701_cov_92.816331"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4158)
                     /locus_tag="PKU82_08780"
     CDS             complement(<1..4158)
                     /locus_tag="PKU82_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83006.1"
                     /translation="MKTTIKYLTLFFALITINVINVSAQCNVGTLAGTITPTINWQTV
                     AVDGRDYQNFAATAGYKYIFSFCAADGGSTSRNTEITINDNAGVIIPGAYNNDFCGSA
                     SYLEWTCVTTGTYRVLTTRRTCATQSNLGTMAYRLDFSYNCPAGLGTGYTSVASLPYT
                     MASGTTCSQKNDVNAIRMEACGTANYYEGEDMVWSFTPATTGTVTVSFNSGASNTTLS
                     IFDACPLKGNGAGCVVNQQGNGNKSVSFCATAGTTYYAVIDCRTSTGCFAFTNFTISA
                     PVVNYNLGNGVTNVASLPFIANNQSTCGNGNDLTSGNTPACGNGLYKAQDDNVYVFTP
                     ATSGDISIRLTSTANNTGLFLYDGSPVPAPCGGSTGVCVASQTGNDDKTLCVTVTAGT
                     TYYLVVDSRTACFAFKLRISAPSTDYSGRTCASPAVIASLPYNKYDETTACFGDDYNN
                     SSVSSCGTTYESGEDKVYSYTTTSARCISITISGASTNNIGYQVYNGCPDAPGTTCIG
                     NLGGATSGFISGSVTLPAAGTYYIIVDTWADPMNADYKIAIASISSVYNDLPCNAQSM
                     ELGLYYTGNTHCADGSGEPAAPGCWSAPNTVNSVWYSFIAPASGNVRVRTSPGTIRRT
                     QIAAYSGVCGSGMALVACNLNAPGCGVAVPNTPTTMSELSLAGLIPGNTYYVVVDGEN
                     YSTGTFSIIAIDGAGTMPDIEGQECSTPIIVCNPNTQVGDPGFQLFGNRCDFPGAGTN
                     CLTRAERGSAWYQINIASAGNLEFNLIPNDWVGAPSTACTDYDFAVWKIAGTGATTCA
                     GIAAGATPVKCNYSPLGITGLYSGSNGTAPPAYPGFGGAYQSRLSVAAGEVYILVVSN
                     YSNSISGFTLNFPASAPVDYTAGGTTAYWTGAESNDWFTAANWGGCNIPSCTVDAVIP
                     LSSANQPYIGTAGATCKDLKLEPGGTLIMDNNQFLSICGNVQNNGELNIATGSGFLFG
                     NGSVNQTLNGNLVSPNRFSHITVNKTGGKVDLLQNIEMQGSLALIGPSSRFDAINKTV
                     TLKGSLSNSAGQFNMGNNGNLVLTGTSTQNISNNDTLTNVIVNKSGNTVVLIRDMKIG
                     PTGSLSLIASKIQTTSANEVYVMNDAASAVSAGSSSSYIEGNLRRNVPTIVSPRVYDF
                     PVGNATKGYQRLNLNLYNGISPAVGSIQASFSDYLTDIWGLGLDAPCLMNYNDTMLNN
                     GYWTLTPANTANGEANITLYNRTYTNAKTNYGVGINPNGAGWMIPPVISGGCVTPPVT
                     AVLRNGVGLSFTSGQSILVGTAQGKVSPLPVELLSFEATAGKQSIFLTWSTASEKNNA
                     GFYLERSTDGNNFDNITWVDGHGTTNQVNNYSYEDKNVKEGVLYYYRLKQIDYDGKFE
                     YSFVKTAM"
     gene            complement(4161..4523)
                     /locus_tag="PKU82_08785"
     CDS             complement(4161..4523)
                     /locus_tag="PKU82_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83007.1"
                     /translation="MKKLVFMVLLMLTKVAFCQENINGQKLKDYVFGVPQMDLVKQLP
                     DFKNSLKTINGLEFHGFCESQHLLMVRMPESSMDAFSELMDKMELSFNVKKQASFQIA
                     EKVCNAKEEILYSKSINQ"
     gene            4714..5466
                     /locus_tag="PKU82_08790"
     CDS             4714..5466
                     /locus_tag="PKU82_08790"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOZ83008.1"
                     /translation="MSSTDKKIYFISDFHLGAPTSEESLVRERKICQWLDTIKADVSI
                     LFILGDVFDFWFEYRHVVPKGYVRILGKLAEIADKGIPIYLFTGNHDMWMFDYLPKEL
                     GIKLYRQPEEFVFDNKKFLIGHGDGLGPGDHGYKFIKKVFANKFCQWLFARLHPNFGI
                     SLANFWSRKSRQHTGHHDAKYLGDDKEFLVQYIIETEKSKHFDYYIFGHRHLPIDKQI
                     GDSHYINLGDWLSYFTYAEWDGSKLSLKKFEA"
     gene            5477..7321
                     /locus_tag="PKU82_08795"
     CDS             5477..7321
                     /locus_tag="PKU82_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HOZ83009.1"
                     /translation="MKKFNALKFGAWFFLAISILAIVVSFRYLNYVSTGGDWLTIIYL
                     AATVLSHFSALSFLIYGLLFVPVALLFKRKQVLIWCAVVIGSVSLLLFILDTFVYDLY
                     RFHINGFVLDMVFGGAFSEIFVFDFLMYLKVAAFVVLLTAIVYLLARIIWSLFETDKL
                     RGGKQIAYLLVGLLIGSHLLHAWAYATSYRSIQQSSLYFPLYFPLRANKLLSKLGVAA
                     DNQKQEWENLQDGNDKAIQYPLRPLTFDTVTKYPNILFILMDAWQFETFNNEVTPNIF
                     RFSEGKILFNRHYSGSNGTRGGIFSMFYGIPSLYWEDFLINRISPVFLDELQKRNYEM
                     KILASAALVSPEFDKTVFSKINNLRTHTPGKNFTERDENITKDFIEWMNTSAFQKPFF
                     GFLFYDSPHNYNLPYWLPHPFKPYWENVDHTVLNNDFDPVPFYNSYKNTVWFCDSLIQ
                     QVLSKLEEKNLLENTIVVITSDHGQEFNENKKNYWGHTSNFSKYQLQVPCIISWPGKN
                     AEAISRFTSHYDLVPTLMQDVLKCRNLVSDYSFGNNLFDTTEQRVFVAGSKEHFAFID
                     LQKNQINTVNFNGSMTVTDMQLNELNVKPDAKMLNDCVLDLQRYYKPH"
     gene            complement(7324..8223)
                     /locus_tag="PKU82_08800"
     CDS             complement(7324..8223)
                     /locus_tag="PKU82_08800"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOZ83010.1"
                     /translation="MIKPTSFGFNIETSKDNVFQKQNNRLSNQQIQLEAEKESRLLRD
                     KLIDSNINVIYAYENCEHNTPDAVFPNNWFSTHDNDTLVLYPMLAANRRNERNAALIN
                     YLKNSFQIKEVVDMTPFENENKFLEGTGSLVFDHKNKICFASISERTNTELANNINKK
                     LGYQTIILNYTDENNTPVYHTNVCMCIGNGFVIAAIDNLKSKSERTALFEYFETNNLN
                     PILISQQQVYSFCGNALQLQTNTERPVLTMSTTAFNAFSTEQIKTIEKHSDILYSSID
                     TIESIGGGSVRCMLAEIFLNPKS"
     gene            complement(8253..>8701)
                     /locus_tag="PKU82_08805"
     CDS             complement(8253..>8701)
                     /locus_tag="PKU82_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ83011.1"
                     /translation="QNIHDAPHKDLRRARAAAQSIIPTTTGAAKAVTEIFPDLKGMIG
                     GAGIRVPVIDGSITDITCILKHDATVEAINQKFLEASQGSMKGIIEYTTDPIVSVDII
                     GNTHSCIFDSMLTSVLGKMVKVVGWYDNEMGYSTRLAELTALVGKQ"
BASE COUNT         2648 a         1585 c         1563 g         2905 t
ORIGIN      
        1 catagcagtt tttacaaaac tatattcaaa cttaccgtca taatcaatct gcttcaatcg
       61 gtagtagtat agaacaccct ctttaacatt tttatcttcg taactataat tgtttacctg
      121 attggtagtt ccatgtccat caacccaggt gatattgtcg aagttgttgc catcagtact
      181 tctctccaaa tagaatcctg cattattttt ctcactagct gttgaccaag tcaggaaaat
      241 actttgttta ccagctgttg cttcgaatga aagtaattct acaggtaatg gcgatacctt
      301 accttgtgct gtacccacta atatgctttg tccgctagtg aaactcaacc ctacaccatt
      361 tcttaataca gctgttacag gaggagttac gcatccgcca ctaattacag gtggaatcat
      421 ccatcctgca ccatttggat tgataccaac accataattt gttttggcat ttgtatatgt
      481 acgattgtat aaggtaatat tagcttcgcc atttgctgta tttgcaggtg ttaatgtcca
      541 atagccattg tttaacatgg tgtcattgta gttcattaga caaggtgcat ctaatcctag
      601 tccccaaatg tctgttaaat agtcggagaa tgaggcttga atacttccta ctgccggact
      661 tattccatta tataaattca agtttaaacg ttgatatcct tttgttgcat ttccaacagg
      721 gaagtcataa actcttggcg aaacaattgt aggaacattt cgtctgagat taccttcaat
      781 ataggaacta ctcgaacctg cactaactgc agatgcagca tcattcatta cataaacttc
      841 gtttgcagaa gtggtttgta tttttgatgc tattagactt aaacttccgg taggtcctat
      901 cttcatatca cggataagaa ctacagtgtt tccgcttttg tttacaatca cattggtcag
      961 cgtatcattg ttgctgatgt tttgtgtact agtaccggtt aatactaagt ttccattatt
     1021 tcccatatta aactgaccgg cagaatttga taagcttcct ttcaatgtaa cagtcttgtt
     1081 gatggcatca aatcttgagc taggaccaat taatgccaaa cttccctgca tttctatatt
     1141 ttgaagtaag tcaacttttc cacctgtttt gtttacagtg atatgcgaga atctgtttgg
     1201 agaaaccaag tttccattta aagtttgatt tacgctacca tttccgaaaa gaaaccctga
     1261 tcctgttgca atatttaatt ctccattatt ctgcacattt ccacaaatag agaggaactg
     1321 attgttgtcc attattagag ttccacccgg ttcaagttta aggtctttac aagtggcacc
     1381 tgctgttcca atataaggtt gatttgcaga tgaaagtggt atgacagcat ctacggtaca
     1441 acttggaata ttacatccac cccagttagc agcagtaaac caatcattgc tttctgctcc
     1501 agtccaatag gctgttgttc cccctgcagt ataatctaca ggagcagaag cagggaaatt
     1561 taaggtaaac cctgaaatac tattcgaata attacttaca actaaaatat atacttcacc
     1621 tgcagctaca ctaagtcttg attgatatgc tcccccaaaa ccggggtaag ccggtggtgc
     1681 agttccattg gaaccactgt ataaaccagt aattccaaga ggactataat tacattttac
     1741 aggtgtagca ccagcagcaa ttcctgcaca tgttgtagca ccggtacctg caattttcca
     1801 tacagcaaag tcataatctg tacatgctgt acttggcgca cccacccagt cattcggaat
     1861 taagttaaat tcaagatttc ctgctgaagc aatgttaatc tgataccaag cagagccacg
     1921 ttctgctcgt gtaagacagt tggttcctgc accagggaaa tcacatcggt tcccgaataa
     1981 ctgaaatccc gggtcgccaa cctgagtatt tggattacaa acaataattg gagtactaca
     2041 ttcctgacct tctatgtcag gcattgtgcc tgcaccgtca atagcaataa tactgaatgt
     2101 gcctgtagaa taattttcgc catcaactac tacataataa gtatttccgg gaattaatcc
     2161 tgctaacgat aattcagaca ttgttgtagg ggtatttgga acagctacgc cacatccagg
     2221 tgcgttaagg ttacacgcta ctaatgccat acctgaacca caaacaccac tgtaagcagc
     2281 aatttgtgtt cttcttatag ttccggggct agtacgaact cttacatttc ctgaagcagg
     2341 tgcaataaaa ctataccaaa cgctatttac agtatttggt gctgaccaac aacccggagc
     2401 tgccggttcg ccactgccat cggcacaatg tgtattacct gtgtaataca atcctaattc
     2461 catgctttgt gcattacaag gcaaatcatt ataaacagaa ctgatgctgg caattgctat
     2521 tttataatca gcattcattg ggtctgccca tgtatcaaca ataatgtaat atgttcctgc
     2581 tgcaggaagt gttacactac ctgaaataaa tccgcttgta gcaccaccta aatttcctat
     2641 acatgttgtg cctggtgcat cgggacaacc attgtaaacc tgatagccaa tgttgtttgt
     2701 gctggcaccg gaaatggtta ttgaaataca tcgtgctgat gttgttgtat aactataaac
     2761 tttatcttct cctgattcat aagttgttcc acaagaagaa acacttgaat tattatagtc
     2821 atctccaaaa catgcagttg tttcatcata cttgttatat ggtaaagatg caatgacagc
     2881 cgggctggca caagttcgtc cggaataatc agttgaaggg gcagaaattc ttaacttaaa
     2941 agcaaaacat gcagtgcgcg aatcaactac taaataatag gttgttccgg ctgtaactgt
     3001 aacacaaagt gttttatcat cattgccggt ttgtgaagca acgcaaactc ctgtgctacc
     3061 gccacaaggt gcaggaacag gacttccatc ataaagaaat aatcctgtat tatttgccgt
     3121 tgaggttaat ctaatggata tgtcaccaga tgttgccgga gtaaaaacat aaacattatc
     3181 atcctgtgct ttgtataaac catttccaca tgcaggtgta ttacctgaag tcagatcgtt
     3241 accattacca catgttgatt ggttgtttgc aataaatggt aaactggcaa cattggtaac
     3301 gccattgcct aaattgtaat ttacaacagg agcactaata gtaaagtttg taaatgcaaa
     3361 acaaccagta gaagtacgac agtctattac tgcataataa gttgtaccgg cagttgcaca
     3421 aaatgaaact gacttattcc cgttgccctg ctgatttact acacagcctg caccatttcc
     3481 ttttaacggg catgcatcaa atatagagag agtagtattg cttgcgcctg aattaaatga
     3541 tactgtaact gtacctgtag ttgcaggagt aaaggaccat accatatctt cgccttcata
     3601 ataattggct gtaccacatg cttccatacg gattgcatta acatcattct tttgtgaaca
     3661 tgtggtgcct gatgccatgg tataaggtaa agaagctaca cttgtataac cggtacctaa
     3721 acctgcagga caattataac taaaatctaa gcgatatgcc attgtaccta agttgctctg
     3781 agtagcacaa gtccttcttg tggtcaaaac acgataagtg ccggttgtga cacatgtcca
     3841 ttctaagtaa gaggctgaac cacaaaagtc attattatag gcaccaggaa tgattacccc
     3901 tgcattgtcg ttaattgtaa tttcagtatt tcttgatgta cttccaccat cagcagcaca
     3961 aaatgaaaaa atgtatttat aacctgcagt tgcagcaaaa ttctgataat ctctaccatc
     4021 aacagcaaca gtttgccaat taattgtagg agtaatagtg ccggctaatg tgccaacatt
     4081 acattgagca cttacattaa taacattgat tgtaatcaat gcgaaaaata aagttaagta
     4141 tttgattgta gtcttcatac tcattgattt atagatttac tgtataagat ttcttctttt
     4201 gcattacaaa ctttttctgc tatctgaaat gaagcttgtt tttttacgtt aaaacttaat
     4261 tccattttgt ccataagctc tgaaaaagca tccatagaac tttctggcat tcgtaccatt
     4321 agtaaatgct gactttcaca gaaaccatga aactcaagac cattaattgt tttaagacta
     4381 tttttaaaat caggaagttg ttttaccaaa tccatctgcg gaactccaaa aacatagtcc
     4441 tttaattttt gtccgttaat gttttcctga cagaaggcaa cttttgtcag catcaacaat
     4501 accatgaata caagtttttt catattaaaa tgattttaac agtgcttata tacgcccaac
     4561 tttaccaaaa ggttatagtt agaccaaagc aattactttt gtaaggtgtg taatgtttta
     4621 ggcttaatgt tgatagtttt ataatcattt aagccgttta ccttgtagtt tgatatttat
     4681 tacagaaatt agcagcaaat tattttttga aacttgagca gcacagataa aaaaatttat
     4741 tttattagtg actttcatct cggagcacca acaagtgaag agagtttggt gagagagcgt
     4801 aaaatctgcc aatggttaga tacaattaag gctgatgttt caatcctttt tatccttggt
     4861 gatgtatttg atttttggtt tgaataccgt catgttgtgc caaaaggata tgtaagaatt
     4921 ttaggaaaat tggcagaaat agccgacaaa ggaataccaa tctatctgtt tacaggcaat
     4981 catgatatgt ggatgtttga ttatttgcca aaagaattgg gtattaaact ttaccgtcag
     5041 ccggaagaat ttgtatttga taacaaaaag ttcttaatcg gtcacggtga tggcttagga
     5101 cccggtgatc atggatataa gttcatcaaa aaagtctttg ccaataagtt ttgtcaatgg
     5161 ctgtttgccc ggttgcatcc aaattttgga atttctttag ccaacttctg gagccgtaaa
     5221 agcagacaac ataccgggca tcatgatgct aagtatttag gtgatgataa agaatttttg
     5281 gttcagtata ttatcgaaac tgaaaaatca aaacattttg attattatat atttggacat
     5341 aggcatctgc caattgataa acaaataggc gatagccact atattaattt gggcgattgg
     5401 ctttcatact ttacttatgc agaatgggat ggaagtaaac tttctttaaa gaaatttgaa
     5461 gcataggtga ttttccatga aaaaatttaa tgctttaaaa tttggcgctt ggttttttct
     5521 ggctatttca attcttgcaa tagtagtgtc gtttcgttac ttaaattatg tatcaacagg
     5581 tggagattgg ttaactataa tatatctggc tgcaacggta cttagtcatt tttctgcctt
     5641 gtcattttta atttatggtt tactgtttgt tcctgtagca ttacttttta agaggaaaca
     5701 ggttttaatt tggtgtgctg tagtaattgg ttcagtttct ttattactat tcattttaga
     5761 tacttttgtc tatgaccttt acaggtttca tatcaatggt tttgttttgg atatggtttt
     5821 tggcggtgct ttctcagaaa ttttcgtttt tgatttttta atgtacttaa aggtagctgc
     5881 atttgtagtt ttactcaccg caattgttta tttgcttgca cgaattatat ggagtttatt
     5941 tgaaacagac aaactgagag gaggaaaaca aattgcttat ttgttagttg gacttttaat
     6001 aggcagccat ctgcttcatg catgggctta tgcaacttct tatcgctcaa ttcaacaatc
     6061 atcactttat tttccattgt attttccatt gcgtgccaat aaacttctca gtaaacttgg
     6121 agttgcagct gataatcaaa aacaagaatg ggaaaatttg caagatggaa atgacaaagc
     6181 aattcaatat ccattaagac cactaacatt tgatactgta actaaatatc ctaacatatt
     6241 atttatttta atggatgcct ggcagtttga aacttttaat aatgaagtaa ccccaaatat
     6301 ttttcgtttc tcagaaggaa aaatcctatt taaccggcac tacagcggaa gcaatggaac
     6361 tcgtggcggc atcttttcta tgttttatgg aataccgtct ttatattggg aagattttct
     6421 gattaacaga atcagcccgg tttttttgga tgagttacag aaaagaaatt atgaaatgaa
     6481 gattcttgct agtgcagcct tggtaagtcc tgagtttgat aagactgttt tttcaaaaat
     6541 taataacttg cgtacacaca ctccgggaaa aaattttaca gagcgtgatg aaaatataac
     6601 caaagacttt attgagtgga tgaatacatc tgcttttcaa aaaccatttt ttggtttttt
     6661 gttttatgac tcaccacaca attataattt accttactgg ttgcctcacc cttttaaacc
     6721 ttattgggaa aatgtagatc ataccgtgct taacaatgat tttgatcctg ttccatttta
     6781 taacagttat aaaaatacag tttggttttg tgattcattg attcaacaag tattgtctaa
     6841 acttgaagaa aagaatttgt tggagaatac cattgttgta attacctcag atcatggaca
     6901 ggagtttaat gaaaacaaga aaaattattg gggacatacc agcaattttt ccaaatacca
     6961 acttcaggtt ccttgtataa tatcatggcc gggaaaaaat gctgaagcaa taagtaggtt
     7021 tacatcccat tatgatttag tccctacact aatgcaagat gtattaaagt gcagaaattt
     7081 agtttcggat tattcattcg gtaataattt gtttgataca acagaacaac gggtttttgt
     7141 tgccggaagt aaggagcatt ttgcatttat tgatttgcag aaaaatcaga ttaatacagt
     7201 taattttaat ggttcaatga cggttacaga tatgcaattg aatgaattaa atgttaaacc
     7261 tgatgcaaaa atgctgaacg attgtgtttt ggacttgcaa cgatactaca agccacatta
     7321 gaatcagctt ttagggttaa gaaaaatttc tgccagcatg caacgtacac taccaccacc
     7381 aattgattca atagtatcaa tggaggagta taggatgtct gaatgttttt caatagtttt
     7441 tatttgttct gttgaaaatg cattaaatgc agtagttgac attgtaagta ctgggcgttc
     7501 agtattagtt tgaagttgta aagcattgcc acagaaagaa tacacctgtt gttgtgaaat
     7561 cagaatgggg ttcagattat tagtttcaaa atattcaaac agtgcagtac gttctgattt
     7621 tgatttcaga ttatcaattg ctgcaataac aaaaccgtta ccaatgcaca tacatacatt
     7681 agtatgatag actggtgtgt tgttttcatc agtgtaattc agtattattg tttgataacc
     7741 taattttttg ttgatattat ttgccaactc agtatttgtt ctttctgaga ttgaagcaaa
     7801 acaaattttg tttttgtgat caaatactaa gctgcctgtt ccttctaaaa atttattttc
     7861 attttcgaat ggagtcatgt caacgacctc tttaatttga aaagaattct ttaaataatt
     7921 tataagtgca gcattccgtt cgtttcttct gttagcagca agcattggat aaagtaccaa
     7981 agtatcatta tcatgagtac taaaccaatt gttcggaaac acagcatcag gtgtgttatg
     8041 ttcgcagttt tcataggcat aaatcacatt gatgttgcta tcaattaatt tatctcttaa
     8101 caaacgactt tccttttcag cttcaagctg tatttgctga ttgcttaatc tgttattttg
     8161 tttctgaaag acattgtctt tgcttgtttc tatgttaaaa ccaaaagaag ttggctttat
     8221 cataagaaca gttgatgcaa agtgcatatt gtttattgct tgccaactaa tgctgtcagt
     8281 tcagctaaac gtgtgctgta gcccatttca ttatcgtacc agccaacaac tttaaccatt
     8341 ttaccaagta cagatgtgag cattgaatca aaaatgcatg aatgtgtatt gccgataatg
     8401 tcaacagaca ctattgggtc ggttgtgtat tcaataatgc ctttcataga gccttgcgat
     8461 gcttccaaaa atttttgatt tatagcttca acagtggcat cgtgtttaag aatgcaggtg
     8521 atatctgtta ttgaaccatc tattaccgga actctgattc ctgcaccacc aatcatacct
     8581 ttaaggtcag gaaaaatttc tgtcactgcc ttagcagcac ctgttgttgt gggaataatg
     8641 gattgtgcag cagcacgtgc ccttcgtaaa tctttatgtg gagcatcgtg tatattttga
     8701 t
//
LOCUS       DAOOOL010000105         8635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_785_length_8635_cov_74.063753, whole genome shotgun sequence.
ACCESSION   DAOOOL010000105 DAOOOL010000000
VERSION     DAOOOL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_785_length_8635_cov_74.063753"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKU82_08810"
     CDS             complement(<1..329)
                     /locus_tag="PKU82_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83012.1"
                     /translation="MYLPITELCCRLILTGIASSFRFKFTTFGAFPFSKTFAVVQNSQ
                     SIAGNYNLTDITLDQLQAQEKEVVVSNEDLTVKVIPLVVPPTDTMPQQQHITETVKEI
                     YTVQKEYV"
     gene            456..728
                     /locus_tag="PKU82_08815"
     CDS             456..728
                     /locus_tag="PKU82_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOZ83013.1"
                     /translation="MSVKQAEEIKESKKSQSNDHHIVLYNDDVNTFDFVIDSLMEICN
                     HEREQAEQCSIIVHNNGRCSVKDGSVKTLRPMCKALVERGLTAEVE"
     gene            809..1606
                     /locus_tag="PKU82_08820"
     CDS             809..1606
                     /locus_tag="PKU82_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOZ83014.1"
                     /translation="MKGLIKILLLIFFVSCSNVPLTERKQINLLPESQMVAMALTSYS
                     DFLKEHPPVVNSNADAQMVKRVGERVKDAVVQYMRDHNLYDRIKDYKWEFNLVDSKDV
                     NAWCMPGGKVVVYSGLLPVTKNEESLALVMGHEIAHAIARHGNERMSQQLIAATGGLA
                     LDVALMNKPAETRQLFNTAYGVGANLGVLLPYSRLHETEADKLGLIFMAMAGYNPAIA
                     PDFWKRMAAVSGNKVPEILSTHPSSETRIHDLNKFMPTAMKYYKPRP"
     gene            1720..2451
                     /locus_tag="PKU82_08825"
     CDS             1720..2451
                     /locus_tag="PKU82_08825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ83015.1"
                     /translation="MTIGKLEFEQAYIKLRKSEGRLYSDAEVALLPEVAKGHTHFKEW
                     KVRKDSLMRLLNYLSRSGNVKNILEIGCGNGWLCNRLAQSGFDVTGLDINQTELEQAK
                     RVFPEINFFYKDIFNSRSEEKFDVVLFAASLQYFRNVNEVILLSMKEFLNENGCVIIA
                     DTKFYTQTECLKAEKRSKLYFEENNSEMSEYYFHHSIDGLNQFEVKVLKPENTFVKLI
                     SGFKNPFNIYIVKLKPQFIESNLQL"
     gene            2429..3358
                     /locus_tag="PKU82_08830"
     CDS             2429..3358
                     /locus_tag="PKU82_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOZ83016.1"
                     /translation="MNQIYNCNSDEVRLSILKVLTYFRIFSHPLKLQEIALLCSVSEK
                     IAEQSLQVLIADEIVYCFDKHFTTCKEVAQLAELRFVKEAEAEKYFAKLKKYARLLST
                     FPFVRGIAVSGSLSKGVMNPDGDIDYFIITKPGRLWICRTSMVLFKKLFLFNSKKYFC
                     VNYFVDSKNLQIRDENIFTATEFIHLLPVFEKDGVLADFVFQNEWVNKFYQRKPDWNK
                     SYMVKGNSRVRSLLEFFLQLSVFNPIERVCHRLTILFWRKKFSTFKKEKFDLTMRSTQ
                     GISKHHPSDFQTKVLKEFQEEISKIHSVTLAEI"
     gene            3355..4782
                     /locus_tag="PKU82_08835"
     CDS             3355..4782
                     /locus_tag="PKU82_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOZ83017.1"
                     /translation="MKVLITHSYFLHFDKKQLELSKPYPPLATLLAASVLRNHSLEVH
                     FYDVSFDETVEKVKEQINVLKPDVLLIYDDGFNYLTKMCLTNMREAAFRMLHFAKSKG
                     IKTIISSSDSTDEYKKYLDAGADVVLLGEAEETLIELFNNRLEVNEDIKGIAFTNNNI
                     FYNTVRRNVNTQLDQLPFPAWDLLNIKPYKKAWEKHGYFSINLSTTRGCPYKCNWCAK
                     PIYGNRYNVRSTENVLNELKYMNRFFKFDHVWFCDDIFGLRPGWLDSFCSRIKESGIN
                     VKYKIQCRADLLQREELIQKLAESGAEEVWIGAESGSQKILDAMDKGITVEQIYHAVT
                     LLKKYKIKPCLFLQFGYPGETKADIQLTLKMLKELDPYDIGVSVSYPLPGTKFYENVK
                     ETMKEKTNWSDSDELLLMYSGEYNPSFYKTLHRYVHKSFRAGKGQQELKKLIMLRRGS
                     LQKALMTAYYKPASLFLKTKINLNQ"
     gene            4779..5573
                     /locus_tag="PKU82_08840"
     CDS             4779..5573
                     /locus_tag="PKU82_08840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ83018.1"
                     /translation="MIKQVMNTSTKAFSKQAAHFDEYNAINCITQYCRKEIYKIAEQH
                     LAPNSSILELNCGTGIDAIYFSKNHRVLATDGATGMIEALNKKISDNEITDIQSMQIS
                     FDQLDKLGKQKFDFIFSNFGGLNCTENLADVIKKLPALLKENGKIMLVIMPPICFWEH
                     LHVLSFNFKLAFRRWKKAGARASVEGVEFKTFYYSPERICRIVKKELKVVYLQGLCNV
                     MPPEYMYNHLKNHPFAFNLLAKADRFVNRFFPFNKTGDYFVIVLEK"
     gene            5580..6968
                     /locus_tag="PKU82_08845"
     CDS             5580..6968
                     /locus_tag="PKU82_08845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOZ83019.1"
                     /translation="MHSVSKQFYIIVILIAAGIFFITGYFAFSGLYGQDAYSYLLQSK
                     NLFTGDYHLNFYPPVYAFSGWVLQLVFQNYAVSLQLVSLIAVVVSTILVWKIVVLQYN
                     NERHALSFSIIIFLLSPFMLRASVLVMSDALAMMFCVACLFFYNIKSPSSTFWIYLFF
                     TLSILTRYATAPLLTPVLIFNLWHSIKRKQFVYLIGGVGMMLVLLGSNFLFWSTEAGG
                     AKHQWLHLWQIQNFFKSSFSTPDGIQDYFLPNVLFPLKALAYPGFMPLFFILLFFLRK
                     GDVLNNRMLVPLLSLLIYFIFLSGIPFQNDRFLVIAYPFVALIMYPAFSRILERFKPS
                     LLIVFMVCIVQITLAFRAVLPMYYRSIIENEIANALEPYQGKILYSFDMDIALKGKGL
                     NFEYRNLNDDKIANFEDGSLLLFNESKFQKQWKGMNPMLNFKFIQENCLLDHEKDFSD
                     DWKLYLIKPKQK"
     gene            6965..8014
                     /locus_tag="PKU82_08850"
     CDS             6965..8014
                     /locus_tag="PKU82_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ83020.1"
                     /translation="MNVIWILPGFASNESDSTCIPPLQDLALSLQQQKVNLTILALDY
                     PFEQKSYCWNGIRVYAVGGANKKRFSRMLLLLRAWRLLISLINNHGVDIIHSFWLTWP
                     AILGQQFCKKYKLPHLQTIMGQDAMKNKFYRLFDFKYSTIVNLTQFANERFYSAISQQ
                     SDEIIPFGLGQVFEEVVDRDIDLINVGSVTGLKNQMFFLKLVYQIHQIKPNIKAVIIG
                     KFYDMHLIKEMKQFIVEKDLESNISLLNELPRVEIFKFMRRSKVMAHTSAFESQGMVM
                     LEAIAHGCKVYSSGKGIHFNHELYSLLTDDLKENVNSIVSYLIKKEYPYGEKLISIEQ
                     TANCYLELYKKLSKK"
     gene            complement(8020..>8635)
                     /locus_tag="PKU82_08855"
     CDS             complement(8020..>8635)
                     /locus_tag="PKU82_08855"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOZ83021.1"
                     /translation="SRSEEWFLYVCMAVSFLGLLMRIYIVGHTPANTSGRNTAEGQVA
                     DTINQTGMYSMVRHPLYVGNFIMWLGIALFTASIWFVLLFIAVYWLYYERIMYAEEQF
                     MRKKFGNAYTDWAEKTPAFIPEFSNFKKPSLPFSWKKVLKKEKNGFAAVFIVFLLFYA
                     AGQLITNNAIQNNFLVYGTVFSILFYIVIKILRKQTTLLNETGR"
BASE COUNT         2860 a         1231 c         1654 g         2890 t
ORIGIN      
        1 acatattcct tttgaactgt ataaatctcc ttcacagttt ctgtaatatg ttgttgttga
       61 ggcattgtat cggttggcgg cacaaccagt ggaataactt ttacagttaa atcttcatta
      121 gaaacaacca cttctttctc ctgtgcctga agttgatcaa gagtaatatc cgtaagatta
      181 taattacctg ctattgactg agaattttgt acaacagcaa aagttttaga gaatggaaat
      241 gctccaaaag tggtaaattt aaatctaaaa gaggaagcta tacctgtgag tatcaggcga
      301 caacacaact cagtaatagg taaatacatt cctgtagtca tcaattaaat atttttcttt
      361 ttatgaagaa atcggtcttt ttcctgattt ttctccattg atttttgaaa tttgattttg
      421 cttgttatta ttgcactgtt caacaaagaa acatcatgtc ggtaaaacaa gctgaggaaa
      481 ttaaagaaag taaaaaatcg caatcaaacg atcatcatat cgtattgtat aatgatgatg
      541 tgaatacttt tgattttgta atagattctt taatggaaat ttgtaatcat gaaagagaac
      601 aagcagagca gtgttcaatt atagttcata ataatggcag gtgttctgtt aaagatggct
      661 ctgtaaagac gttgaggcca atgtgtaagg ctttggttga acgtggactt acagccgagg
      721 tagaataagt caggattttc attcggaatt tattttgtat gcataccgaa agcagaatta
      781 ttaagtttta gtttaaagcg taaatcacat gaaaggttta ataaaaatat tgttgctgat
      841 cttttttgtt tcatgcagta atgttccgtt aacagagcga aaacaaataa atcttctacc
      901 ggaatcgcag atggttgcta tggccctaac atcatacagc gattttctta aagaacatcc
      961 tcctgtagta aatagtaatg cagatgcgca aatggttaaa agagtaggtg agcgtgttaa
     1021 ggatgccgtt gttcaataca tgcgcgatca taatctatac gatagaataa aagactacaa
     1081 gtgggaattt aatcttgttg acagtaaaga tgtaaatgca tggtgtatgc ctggaggtaa
     1141 agttgttgtc tattcgggat tattacctgt aacaaaaaat gaggaaagcc ttgccttggt
     1201 tatggggcat gaaattgcac atgctatagc tcgccatgga aatgaaagaa tgagtcagca
     1261 attgatagca gcaacaggtg gtctggcatt agatgttgca ttgatgaata agcctgcaga
     1321 aacaagacag ttgtttaata cagcctatgg tgttggtgca aaccttggcg tactattacc
     1381 ttactcacgc ttgcatgaaa cagaagccga taaactggga ttaatattta tggcgatggc
     1441 aggttataat cctgcaatag cacctgattt ttggaaaaga atggcagcag tatcgggtaa
     1501 taaagttcct gaaatactaa gcacacaccc aagtagcgaa acacgtattc acgatttgaa
     1561 taagtttatg cctacagcaa tgaagtacta taaacctcgt ccatagttat tgtttttttg
     1621 gaaaggaatt tctacacatc ttattttaac atttataatt tctgaagaat atttaggatt
     1681 aatagtttta cgcctgaaat attttgatca tgtagatata tgactatcgg caaacttgag
     1741 tttgaacagg cgtatattaa attgcgtaaa tcggaaggca gattgtattc tgatgcagag
     1801 gttgcattac ttcctgaagt agcaaagggg cacacacatt ttaaagaatg gaaagtaaga
     1861 aaagattctt tgatgcggct actaaactat ttaagccggt ctggtaatgt gaaaaatatt
     1921 ctggaaattg ggtgtggaaa tgggtggcta tgtaatcggt tagcgcaatc aggattcgat
     1981 gttacaggtc tcgatatcaa tcaaacagag ttagaacaag ctaaacgtgt atttcctgaa
     2041 attaattttt tctacaagga tatttttaat tcccgttcag aagaaaagtt tgatgttgta
     2101 ttatttgctg catcattgca atattttaga aatgtaaatg aggtaatcct gctcagcatg
     2161 aaagagttct tgaatgaaaa tggttgtgtt atcattgcag atacaaaatt ttatacccaa
     2221 acagaatgtc ttaaagcaga gaaaagaagt aagttgtatt ttgaagaaaa taattcggaa
     2281 atgagtgaat attatttcca ccattctata gatgggctaa atcaatttga agttaaagtg
     2341 ctgaagcctg aaaatacttt tgtcaagctt atatcaggat ttaaaaatcc gtttaatatt
     2401 tatattgtta aattaaaacc gcaatttatt gaatcaaatt tacaattgta attccgatga
     2461 agttcgattg tcaatattaa aggtgttgac ttattttcgg attttctcac atccattaaa
     2521 gctacaagaa attgcattgc tttgtagtgt ttctgagaaa atagctgaac agagtttgca
     2581 agtattgatt gctgatgaaa tagtatattg ttttgataaa cattttacta cctgtaaaga
     2641 agtggcacaa ttagccgaat tgcgctttgt gaaagaagct gaggcagaaa agtattttgc
     2701 taaacttaaa aagtatgcaa ggttgttatc aacttttcct tttgtacgag gaattgctgt
     2761 ttcgggttca ctgtcaaaag gagtaatgaa ccctgatggt gatattgatt attttatcat
     2821 aaccaaaccg ggcaggcttt ggatttgcag gacatcaatg gtgttgttta agaagctgtt
     2881 cttgtttaat tctaaaaagt atttctgtgt gaattatttt gttgattcaa aaaaccttca
     2941 gattagagat gaaaatattt ttactgccac agaatttatt catttgcttc cggtatttga
     3001 aaaagatggt gtacttgctg attttgtttt tcaaaatgag tgggtaaata aattctatca
     3061 gcgaaagcct gactggaata agagttatat ggtgaaggga aattcaagag taagaagttt
     3121 attagaattt tttcttcagt tatctgtttt taatccgatt gaacgagttt gtcatcgctt
     3181 aactatttta ttttggcgta aaaaattttc aactttcaaa aaagaaaagt ttgatctgac
     3241 tatgcgaagc acacaaggta tttcaaaaca tcacccaagc gattttcaga ctaaggtatt
     3301 aaaagaattt caagaggaga ttagtaaaat tcattcagtt acgcttgcag aaatatgaaa
     3361 gtgttaatca ctcatagtta tttcttacat ttcgacaaga agcaactcga attatcgaaa
     3421 ccgtatccac ctttggctac cttgctagca gcaagtgttt tgcgtaatca tagccttgaa
     3481 gtgcattttt atgatgtaag ttttgatgaa actgtagaaa aagttaaaga acaaatcaat
     3541 gttcttaaac ctgatgtttt actgatttat gatgatggat ttaactatct tacaaagatg
     3601 tgccttacca atatgcgtga agctgctttc aggatgttgc actttgcaaa gtcgaagggc
     3661 attaagacta ttatatcaag cagtgattca actgatgaat ataaaaagta tttggatgca
     3721 ggagcagatg tagttttgtt aggagaggct gaagagacat taattgagtt atttaataac
     3781 agactggagg taaatgaaga tataaaggga attgcgttta caaataataa tatcttctat
     3841 aatactgtaa gaagaaatgt taatacgcag ttagatcaat taccatttcc agcttgggat
     3901 ttgttgaata ttaagcccta taaaaaggca tgggaaaagc atggctattt ttcaattaac
     3961 ctttcaacta ccagaggatg tccatataaa tgcaattggt gtgcaaaacc tatttacggc
     4021 aacaggtata atgttcgttc aactgaaaat gtattaaatg aattaaaata catgaaccgg
     4081 ttttttaagt ttgatcatgt atggttttgt gatgatattt ttggactgag acccggatgg
     4141 ttagattctt tttgtagtcg tatcaaagaa tctggaatca atgtaaaata caaaattcaa
     4201 tgtcgtgcgg atttgttaca gcgtgaagaa ttaatacaaa agttagcaga aagtggagcc
     4261 gaagaagttt ggataggtgc agagtcgggc tcacagaaaa ttcttgatgc catggataaa
     4321 gggataactg ttgagcaaat ttatcatgct gtcacacttc ttaagaagta taaaatcaag
     4381 ccctgtcttt ttcttcaatt tggttatccc ggtgaaacaa aagctgatat tcaactcaca
     4441 ttaaagatgc tgaaagaact agatccttat gacattggag tctcagtcag ttatccattg
     4501 cccgggacta aattttatga gaacgtaaaa gaaacaatga aagaaaagac taactggagt
     4561 gattctgacg aattgttatt gatgtactca ggtgagtata acccatcttt ttataaaaca
     4621 ttacatcgtt atgtgcataa atctttccgt gcaggaaaag gacaacaaga acttaagaag
     4681 ttgataatgc ttcgtagagg aagtttgcaa aaagcattga tgactgcata ttataaacct
     4741 gcaagtttat ttcttaaaac aaaaattaac ttgaaccagt gataaaacaa gttatgaata
     4801 catcaactaa ggcattctca aagcaagctg cacattttga tgagtataat gcgattaatt
     4861 gtattacaca gtattgtaga aaagaaatat ataaaattgc agagcaacat ttagcgccca
     4921 atagtagtat tctggaactg aattgtggaa caggaattga tgcaatttat ttttcgaaaa
     4981 atcatcgtgt tttagctact gatggagcca cgggaatgat agaggcattg aataaaaaaa
     5041 ttagtgataa tgaaattaca gatatacagt caatgcaaat ttcttttgat cagttagata
     5101 aattaggaaa gcaaaaattc gattttattt tttctaattt cggaggattg aattgtacgg
     5161 agaatttggc tgatgttata aaaaaactgc ctgcattact caaggaaaat gggaagatta
     5221 tgcttgtaat aatgccacca atctgctttt gggaacattt gcatgtatta tcatttaatt
     5281 ttaaacttgc tttcagacga tggaagaaag ctggggcaag agcaagcgtt gaaggagttg
     5341 aatttaaaac attttattat agtcctgaaa ggatttgccg cattgtgaaa aaggagttaa
     5401 aggtagtata tctacagggt ttgtgtaatg taatgcctcc ggaatatatg tacaatcatt
     5461 taaagaacca tccttttgct tttaatttac tagctaaagc agatcggttt gtcaatcgct
     5521 tttttccgtt taataaaaca ggagattatt ttgtaattgt cttggaaaag taacagaaaa
     5581 tgcacagtgt aagcaaacaa ttttacatta ttgtaatatt aattgccgca ggcatatttt
     5641 ttatcaccgg atattttgcc ttcagtggtt tgtatgggca ggatgcttat agttatttgt
     5701 tgcagagcaa gaatttattt accggtgatt atcatttaaa tttttatcct ccagtatatg
     5761 cattctccgg atgggtgttg caacttgttt ttcaaaacta tgcagtttct cttcagttgg
     5821 tcagcctgat tgcagttgtt gtatcaacaa ttttggtttg gaagattgtt gtactgcaat
     5881 ataataatga gcggcatgct ttaagtttta gtattattat ttttttactt agcccgttta
     5941 tgttgcgtgc atccgtgctt gttatgagcg atgcattggc tatgatgttt tgtgttgcct
     6001 gccttttctt ttataatata aagagtcctt cttctacttt ttggatttat ttatttttca
     6061 cactttcaat tttaactcgt tatgctacag ctccattact tactccggtt ttaattttca
     6121 atttgtggca ttcaattaaa agaaaacaat ttgtttattt aataggaggt gtcggtatga
     6181 tgctggtttt acttggatca aattttttat tctggtcaac agaagcagga ggggctaagc
     6241 atcaatggtt acatttgtgg cagattcaaa attttttcaa gagcagtttc tcaacgccag
     6301 atggtattca ggattatttt ttacccaatg tgcttttccc attaaaagcg cttgcatatc
     6361 cagggtttat gcctttgttt tttatattgt tattcttttt acgaaaaggt gatgtattga
     6421 acaatagaat gttggtacca ttattatctc ttctgattta ttttattttc ctttctggca
     6481 ttccgtttca gaacgatagg tttctggtaa ttgcatatcc ttttgtagca ttaatcatgt
     6541 atccggcatt tagcagaata ttggagcggt ttaaaccttc ccttttaatt gtttttatgg
     6601 tatgtattgt tcaaattact ttagcctttc gagcagtttt gccaatgtat tatcgaagca
     6661 ttattgaaaa tgagattgca aatgcattag aaccatatca gggaaaaatt ttgtattctt
     6721 ttgatatgga tattgcattg aaaggaaagg gcttgaattt cgaatatagg aatttgaatg
     6781 atgacaaaat tgcgaatttt gaagatggga gtttactttt atttaatgaa tcaaaatttc
     6841 aaaaacaatg gaagggaatg aatccaatgt tgaatttcaa attcatacaa gaaaattgtt
     6901 tattggatca tgagaaagat ttttctgatg attggaaatt gtacctgatt aagcccaaac
     6961 aaaagtgaat gtaatctgga tattacccgg ctttgcgtca aacgaatcgg attcaacctg
     7021 tattcctcca ttacaggatt tggctttatc attgcaacaa cagaaagtta atttaacgat
     7081 tcttgctctt gattatccat ttgagcagaa aagttattgc tggaatggta tcagggttta
     7141 tgctgtagga ggtgcaaata aaaagcgttt ttcaagaatg ttattgttgt taagagcatg
     7201 gaggttgcta ataagtctta taaataatca tggtgttgat ataatacatt cattctggtt
     7261 aacatggcct gcaattttgg gtcagcagtt ttgtaaaaag tataaactac ctcatctgca
     7321 aactatcatg ggtcaggatg caatgaagaa taaattttac aggttgtttg atttcaaata
     7381 ctcaacaatt gttaacctta cacagtttgc caatgaaaga ttttattcag ccataagtca
     7441 acagagtgat gaaataattc catttggact aggtcaggta tttgaggaag tagttgatcg
     7501 agatattgat ttaataaatg ttggctcagt gacagggttg aagaatcaaa tgtttttttt
     7561 aaagttagtg tatcaaattc atcaaatcaa accaaacatc aaagctgtaa taatcggcaa
     7621 gttttacgat atgcatttga ttaaagagat gaaacaattt attgttgaga aagatttaga
     7681 aagtaatata tcgcttttaa acgaattgcc ccgagtggaa atttttaaat ttatgcggag
     7741 gtcaaaagta atggcccata catctgcatt tgaaagtcag ggtatggtaa tgcttgaagc
     7801 tatagcgcat ggttgcaaag tatattcatc aggcaaagga atacatttca atcatgaatt
     7861 atattctctt cttactgatg atttgaaaga gaatgttaat tctattgtta gttatttaat
     7921 aaagaaagaa tatccttatg gagaaaaact gatttctatt gaacaaacag ctaattgtta
     7981 tttggagctt tataaaaagc tatctaaaaa ataatttttc taacgaccgg tttcgttcaa
     8041 taatgtagtt tgctttctga ggattttaat aactatataa aataagatac tgaaaacagt
     8101 tccgtaaact aaaaaattgt tttgaatagc attgttggtt attaattgac cagctgcata
     8161 aaagagtaaa aagacaatga aaaccgcagc aaatccattc ttttcttttt ttagaacctt
     8221 tttccaacta aatggaagtg atggtttttt aaaatttgaa aattcaggaa taaaggcagg
     8281 cgttttctct gcccaatcgg tgtaggcatt gccgaacttc ttgcgcataa attgttcttc
     8341 tgcatacatt atacgctcat agtacagcca ataaacagca ataaacaata agacaaacca
     8401 aatacttgct gtaaacaatg ctatgccaag ccacatgata aaattaccaa catacaatgg
     8461 atgacgaacc atcgaataca ttcctgtttg attaatggta tcagcaacct gaccttcagc
     8521 tgtattgcga ccagaagtgt ttgccggagt gtgtcctaca atataaattc tcatcaataa
     8581 gcctaaaaac gatacagcca tacaaacgta taaaaaccat tcttcagacc ttgaa
//
LOCUS       DAOOOL010000106         8384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_820_length_8384_cov_161.599232, whole genome shotgun sequence.
ACCESSION   DAOOOL010000106 DAOOOL010000000
VERSION     DAOOOL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_8384_cov_161.599232"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKU82_08860"
     CDS             complement(<1..543)
                     /locus_tag="PKU82_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83022.1"
                     /translation="MEIILATPYWYILLCLLAGLGCAWWLYGGNSPFNETPWLRRTLF
                     FLRFLSFSIIAFLLLSPFIKSAIREVEKPAIAIAFDNSSSILLDSTHFSASALNQLAE
                     KLTSAFKNDYEAGRYSFGSKVDNEVTDNDFSEKSTNISEMFDYLKSLYGGRNLGAVIM
                     LSDGIYNKGSNPVYTAEKLKV"
     gene            600..1256
                     /locus_tag="PKU82_08865"
     CDS             600..1256
                     /locus_tag="PKU82_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ83023.1"
                     /translation="MSIFIENIHKAYNGLKVLQGISLEIKTNELVAIIGPSGAGKSTL
                     LHIIGTIDKPDSGKVLLNNTDALALSSKKLAAFRNKHIGFVFQFHHLLPEFTSAENVM
                     IPAMIAGESESSALKRSNALLIQLGLEQRMHHKPSQLSGGEQQRVAVARALINNPDFI
                     LADEPSGNLDSGNAEDLHKLFLKLRNDYKKTIVIVTHNNQLAEMADRTITIRDGLLAQ
                     "
     gene            complement(1310..1873)
                     /locus_tag="PKU82_08870"
     CDS             complement(1310..1873)
                     /locus_tag="PKU82_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOZ83024.1"
                     /translation="MEDFNQHAATGSLLISEPLQSDSFFKRSVILLSEHDTEGTLGFI
                     LNKLTDVTINDAVEDFPHFNAPLFFGGPVETDTLFYLHTVGHLLKDSKEIVKGVFLGG
                     DYDELKFLINTKQISPNQIRFYAGYSGWKPNQLEDELFHNAWMLGKGNAEFTFYPEPE
                     VLWSQVLRSMKNKYSLLANFPDDPTLN"
     gene            2077..2724
                     /gene="pdxH"
                     /locus_tag="PKU82_08875"
     CDS             2077..2724
                     /gene="pdxH"
                     /locus_tag="PKU82_08875"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOZ83025.1"
                     /translation="MDDIHKVLKNVRSDYEADTFGTQEADDNPIVQFEKWFSLAAQAN
                     LPDVNAMNLATCTKNGRPSSRIVLLRDATEEGFTFFTNYNSRKGNQLIENPYAALNFF
                     WPELAKQIRVEGKVEKVDTKVSDEYFLSRPLESRIGAWASEQSQIIESRLVLEKKFLS
                     LKESMGSEVMRPPHWGGFILKPDYFEFWQGRQSRLHDRVAYTLNNNQWSKATLSP"
     gene            complement(3001..3603)
                     /locus_tag="PKU82_08880"
     CDS             complement(3001..3603)
                     /locus_tag="PKU82_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83026.1"
                     /translation="MAKASKSKKSAAKKKAKPARKASGKKAAKKKPVAKKKATKKKAA
                     PKKKAATKKKAAPKKKAATKKKAAPKKKAATKKKAAPKKKAATKKKVAPKKKATTKKK
                     AAPKKKAATKKKAAPKVVIKKVVSKPAVKKPAPMPKEKPEDIQPVVTYQTEIIEIVRP
                     VEMDMDDNDAKMDEKEMMDDDVSFNDDLFSGSSDDDDDDM"
     gene            3800..5416
                     /locus_tag="PKU82_08885"
     CDS             3800..5416
                     /locus_tag="PKU82_08885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOZ83027.1"
                     /translation="MLLLLLICIGCSQKPIADLILFNGKIYTVDSTFSTAQAMVVSNG
                     KIVETGTNALADKYQCQQKIDLMGKSVYPGFIDAHCHFYGYSTDLSKCPLFGTKSFDE
                     VIQKVKEYAATNKFSWILGRGWDQNDWSENEFPDKSVLDSLFPNTPVYLMRIDGHAVL
                     VNQKALDIAGITTSTKVQGGEIITKNNHLTGLLIDNAVDIVKAHIPEFTAQIKTEGLI
                     KGEQNCFAVGLTSVTDAGLRVSDIRLLDSLQKAGTLSMKIYAMAELTNESKKYWFEKG
                     KYKTERLEVRSFKLYADGALGSRGACLLQPYSDLPGHYGFILQDKDSIQLAAKEVVEH
                     GFQLNTHCIGDSANRFMLKVYAANLKTKNDLRWRIEHCQVVNHDDLKYFSDYSIIPSV
                     QPTHATSDMYWAEKRLGSERIHDAYSNNALLKETGMLALGSDFPVEDINPLYGFYAAV
                     VRKDQKGFPPEGFETENKISREEALRGMTIWAAYSMFEENEKGSLEKGKAADFVIMND
                     DLMTAPDSILFKLPIETTYSNGIKVYENKK"
     gene            5400..6857
                     /locus_tag="PKU82_08890"
     CDS             5400..6857
                     /locus_tag="PKU82_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83028.1"
                     /translation="MKTKSKIIFFLLLFCSVSAFGQNLIWNPTVKLNDRPYPKFLGVN
                     NGYFYAAGSNIKMIQGASDSIFLATAWKKLKLTLLRYDDKFILKDTAAIRFLQFPVRV
                     YDVVLSDSTIKIFYSKGVGNLEFCADVFDLKGYFKQTVVLIAGKDVAKYNQKNFFSYN
                     TSINNRFFTITTNDSIYCFSNQLVSAWKTSLPNSTFFDGAVNDSGTFSGIFQRDKEYF
                     IATSVEGKIITQTISAEKEELSDFKIIHEGNRIIVVSLFGFTDNTFNPIYNDIANANK
                     FKSNGISISEFDNNLQLIKTNSIKFSEETLLETVDNLLLKNIKGVDFLKIQQMDVMSN
                     GNIMLLIEKQFASYTKPADVNGKKVASIGRETSANELLLICTDMQDKNMQTVIKRKVS
                     ATENFEYVCNVKGIPHKDDYYLYFLDGRNPEEYQMTQSIYNSQLRRIYTKEMDLASAK
                     KLLPDMSSITRLDKNRFILYARLLKKFSTAVLDFK"
     gene            complement(6985..7323)
                     /locus_tag="PKU82_08895"
     CDS             complement(6985..7323)
                     /locus_tag="PKU82_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83029.1"
                     /translation="MKKALSVLSVLGLFLIVNAYANSNKANTVTDLQVTESSIVANHD
                     MPCDGKAEKDKDCCKKKSKKKKCCADKAEASAASCADKQAKAGCCAHKKAGAGASDVK
                     TDTKSDESAK"
     gene            complement(7436..8170)
                     /locus_tag="PKU82_08900"
     CDS             complement(7436..8170)
                     /locus_tag="PKU82_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOZ83030.1"
                     /translation="MSEAGKPIVEVCLTPALFYQYNTKESIVVAIDILRATSSICVAL
                     QYGARSIVPVMTVEESFQYKEKGYLIGAERQGSMVEGFDLGNSPFSYMEDKIIGRDIA
                     LTTTNGTKAINIANGSAGIVAGSFLNLDVLAAWLIEQNKDVLCLCAGWKNTFNLEDTL
                     FAGALSQKLLPHFQLSEHRDSAIAAMNLYNLAKHDMYDFLKDSSHRTRLERLKIDEDV
                     LYCLTPNQTLVVPVLKDGVLKKHSDW"
     gene            complement(8174..>8384)
                     /locus_tag="PKU82_08905"
     CDS             complement(8174..>8384)
                     /locus_tag="PKU82_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOZ83031.1"
                     /translation="IPRHDYEICNASGQKIGRVTSGTQSPTLNKAIGMGYVSKDQSAE
                     GTEIFIKIREKLIKAAVVKIPFIKK"
BASE COUNT         2630 a         1531 c         1568 g         2655 t
ORIGIN      
        1 aacttttaat ttttctgctg tataaacagg attacttccc ttattataaa ttccatcgga
       61 caacataatt acagcaccca aatttcgtcc gccatagagt gactttaaat aatcgaacat
      121 ctctgaaatg ttagtactct tctcagagaa atcattatca gtaacttcat tgtcaacctt
      181 actgccaaaa gaatatcttc cggcttcata atcgttttta aatgctgaag taagtttctc
      241 tgccagttga tttaatgcag atgccgaaaa atgtgttgaa tcaagcaata tggacgaaga
      301 gttatcaaaa gcaatggcaa tagcaggttt ttcaacttcg cgtatggcag atttaataaa
      361 tggtgataac agcagaaaag caatgatgga aaaggataaa aatctaagaa agaataaagt
      421 acgcctcagc caaggtgttt cattaaaggg tgaattacca ccataaagcc accatgcaca
      481 gcccaagcct gccagcagac aaagtaaaat ataccaatat ggtgttgcaa gtattatttc
      541 caaaacaaaa cgatgagggg caaatataat agtattggaa tgatacttat tttagctgcg
      601 tgagcatatt cattgaaaat atacataaag catataacgg gctaaaggtt cttcaaggca
      661 tttcacttga aattaaaacc aatgagttgg tagccatcat aggtccatcg ggtgccggga
      721 agtccacgct gctgcatatt atcggcacaa ttgacaagcc ggatagcgga aaagtgttgc
      781 tcaacaatac agatgcctta gcactttctt caaagaaatt agctgccttc aggaataagc
      841 atattggttt tgtttttcag tttcatcatt tgttgcctga atttacttcc gcagaaaatg
      901 ttatgattcc tgctatgatt gccggggaaa gtgagtcgag tgctttaaag cgaagcaatg
      961 cgttgttgat tcagttaggg cttgaacagc gcatgcatca caaaccatca cagctatcag
     1021 gtggcgaaca acagcgtgtt gccgttgcaa gggcattaat taacaatcct gattttattc
     1081 ttgccgatga accttcggga aacctagatt caggcaatgc agaagactta cataaactgt
     1141 ttttgaaact gcgaaacgat tataaaaaaa ccattgttat tgtgacgcat aacaatcaac
     1201 ttgcagaaat ggccgacagg acaattacaa ttcgtgatgg actgcttgcc caataagttt
     1261 ttacaatgga cttaaataat ttattctgat ttcttataac taatctaact tagttcaaag
     1321 taggatcatc agggaaattt gccagcaaag aatatttgtt tttcatgctg cgcaatacct
     1381 gactccacaa aacttcaggc tcaggataga atgtaaattc cgcattaccc ttaccaagca
     1441 tccatgcatt gtgaaacaac tcgtcctcta actgattggg tttccaaccg gagtagcctg
     1501 cataaaatct aatctgatta gggctgattt gttttgtatt gattaaaaat ttcaactcat
     1561 cataatcacc acctaaaaaa actcctttta caatttcttt cgaatcttta agaagatggc
     1621 cgacagtatg cagataaaac aatgtatctg tttctaccgg accaccaaag aataaaggtg
     1681 cattaaagtg tgggaaatct tctactgcat cgttaattgt aacgtctgtg agtttattta
     1741 atataaagcc taacgtgcct tctgtatcat gttcgcttag taaaataact gagcgtttaa
     1801 aaaatgagtc ggactgtaaa ggttctgaaa ttaacagtga ccctgtagct gcatgttgat
     1861 tgaaatcttc catagaacac taaattaatt tgttatattt tacgtatctg tctgaaaaag
     1921 ttacttgttg ttaacaatct cttttaataa atcagtttgc taaaaacatg ccactgtctt
     1981 ttcaacatgc tgataacaag gctacttatg ttaaagaaag gatgaataat acatatatct
     2041 tacatttata aatttgcatt tcaaatgaca ttaatcatgg atgatataca taaagtttta
     2101 aaaaatgtgc gcagtgacta tgaggcagac acgttcggca cacaagaggc tgatgacaat
     2161 ccgatagtac aattcgaaaa gtggtttagc cttgctgcac aggcaaattt acctgacgta
     2221 aatgccatga atcttgcaac atgtacaaaa aacggcaggc catcatcaag aattgttttg
     2281 ttgcgcgatg caacagaaga aggttttact ttctttacca attacaacag cagaaaagga
     2341 aatcaactga tagaaaatcc ttatgcagca ttaaattttt tctggcctga attggcgaaa
     2401 caaattcgtg ttgaaggcaa ggttgaaaaa gttgacacga aagtttctga cgaatatttt
     2461 ttatcacgcc cattagaaag tcgtattggt gcatgggcat cagagcaaag tcagattatt
     2521 gagtcgcgtt tggtacttga aaaaaagttt ttatcattaa aagaaagtat gggcagcgag
     2581 gtaatgcgcc ctccacactg gggaggtttt attttaaaac ccgattattt tgaattctgg
     2641 caaggcaggc aaagccgcct ccacgacaga gtagcttata cattgaacaa taatcaatgg
     2701 agtaaggcaa cgctgtcgcc ttgaatttag aggtcggaca atttttgagt gatgattagc
     2761 cgcagttgac tggggtgatg atcagccacg gctgacaggg gtgaaacgtg atgaagtaat
     2821 ctgataattc cgaattgtta gttgagaaat ttaaggtaaa aagtaaaagt gacggggaat
     2881 gagaggactg ttgggtagag ttttcttaaa gattaataaa tggataaaaa tcatcatact
     2941 taagtcaaca aaaaaagccc tgcaatgcag agcttttttt aaatattaat tatttacatt
     3001 ttacatatca tcatcgtcat cgtctgatga gccgctgaat aaatcatcgt taaatgacac
     3061 atcatcgtcc atcatttctt tctcgtccat ctttgcatca ttatcatcca tatccatttc
     3121 taccggccta actatttcaa taatttctgt ttggtatgtt accacaggct gaatatcctc
     3181 aggtttttct tttggcattg gcgcaggttt tttcactgcc ggttttgata ccactttttt
     3241 tattacaacc ttaggtgccg ctttcttttt ggtagcagct tttttctttg gagctgcttt
     3301 ctttttagta gtagcctttt tctttggtgc tactttcttt ttggtggctg cctttttctt
     3361 tggtgcagcc ttctttttgg tagcagcctt tttctttggt gctgctttct ttttagtagc
     3421 tgcctttttc tttggcgcag ccttcttttt ggtagctgcc tttttctttg gtgctgcttt
     3481 ctttttagta gccttctttt ttgctacagg tttcttttta gctgcctttt taccacttgc
     3541 tttgcgggca ggttttgctt ttttcttagc agcagatttt tttgattttg atgcttttgc
     3601 cataggtaaa caagattgat ggtgagtatg tgttctattt tttgcgtaaa tatacagcag
     3661 gttttaattg atgcaagaac aaatcaaaaa atatttttca atacctgcct gtttctaagt
     3721 tgcgcctttc ttttaaagat tttatggata tttgatacct gattttaaaa ttctaacatt
     3781 gattaagtcc tttaaaccaa tgctgctgct gcttttaatt tgcataggat gcagccaaaa
     3841 gccaatagca gacctgattc tttttaacgg taaaatttat accgttgaca gcaccttttc
     3901 tacagcacaa gcaatggtgg tttctaatgg caagatagta gaaaccggca ccaatgcatt
     3961 ggcagataag tatcagtgcc agcagaaaat agaccttatg ggtaaaagtg tttatccggg
     4021 ctttattgat gctcactgtc atttttatgg ctacagcaca gacctttcca aatgcccgct
     4081 ttttggcaca aaatcatttg atgaagtaat acaaaaggta aaagaatatg ccgctaccaa
     4141 taagttcagt tggattttag gacgtggatg ggatcaaaat gactggtctg agaatgaatt
     4201 tcccgacaag tctgttttgg atagtttgtt cccaaataca ccggtttatc tgatgcgaat
     4261 tgatggtcat gccgttttag tcaaccaaaa agctttggat attgcaggta taaccacttc
     4321 tacaaaggtt cagggtggtg agataataac taaaaacaac cacttaaccg gactgctgat
     4381 tgataatgct gttgacattg taaaagcaca tattcctgaa tttacagctc agattaaaac
     4441 ggaaggactt ataaaaggcg aacaaaattg ttttgccgtt ggtctgacct ctgttacaga
     4501 tgccggatta agagtttctg acattcgttt actcgactca cttcaaaaag ccggaaccct
     4561 tagcatgaaa atttatgcca tggcagaact gacgaatgag agtaaaaaat actggttcga
     4621 aaaaggaaaa tataaaactg aaagacttga ggtaagatcg tttaaacttt atgccgatgg
     4681 agcacttggg tcaagaggtg cctgcctgtt gcaaccatac tccgaccttc ccggacatta
     4741 tgggtttata cttcaagaca aggactccat tcaattggca gccaaagaag tagtggagca
     4801 tggatttcag ctaaacacgc attgtattgg tgattctgca aaccgattta tgttaaaagt
     4861 ttatgccgcc aacctaaaaa ccaaaaatga tttaagatgg cgcatcgaac attgtcaggt
     4921 tgtaaatcat gatgacttaa agtatttttc tgactattcc ataataccaa gtgttcagcc
     4981 aacacatgcc acaagcgata tgtattgggc agaaaaaaga cttggcagcg aaagaataca
     5041 cgatgcctat tcaaataacg ccttattaaa agaaacaggt atgttagcct taggcagcga
     5101 ctttccggtt gaagacatta acccgcttta tggattttat gcagctgtgg ttcgtaaaga
     5161 tcagaaaggt tttccaccgg aaggttttga aacagaaaat aaaatcagtc gcgaagaagc
     5221 attgcgcggc atgaccattt gggcagcata ttctatgttt gaagagaatg aaaagggctc
     5281 tttagaaaaa ggcaaagcag cagactttgt aattatgaat gacgacctga tgactgcacc
     5341 ggacagtata ttatttaaat tacctataga aacaacttat tcaaatggca ttaaagttta
     5401 tgaaaacaaa aagtaaaatc attttcttcc tcttattatt ctgtagtgtt tctgcctttg
     5461 ggcaaaacct gatatggaac ccgacagtaa aattaaatga ccgtccatac ccaaaatttt
     5521 taggcgtaaa caatggctat ttttatgctg ccggcagcaa tattaaaatg attcagggtg
     5581 ccagcgacag cattttttta gcaacagcct ggaaaaagct caaattaact ttactacgct
     5641 acgatgataa atttattcta aaagatactg cggctataag atttttgcag tttccggtgc
     5701 gggtatatga tgttgtactg agcgattcaa ccataaaaat attttattca aaaggggttg
     5761 ggaatctgga attttgcgct gatgtttttg atttaaaagg atattttaag caaacagtag
     5821 ttttaattgc aggaaaagat gttgcaaaat ataatcagaa gaattttttc agctataata
     5881 cttccatcaa taaccgtttc tttaccatta ccactaatga cagtatttat tgtttcagca
     5941 atcagttagt cagtgcatgg aaaacttctt tacccaacag tacatttttt gatggagcag
     6001 taaatgatag cggcactttt tcaggtatat tccaaagaga taaagaatat ttcattgcaa
     6061 ctagtgttga aggaaaaatt atcactcaaa caatttctgc tgaaaaggaa gaattatccg
     6121 atttcaaaat tattcatgaa ggcaacagaa taattgtggt ttcattattt ggtttcactg
     6181 acaatacttt caatcctatt tataacgata ttgccaatgc aaacaaattt aaaagtaatg
     6241 gtataagcat ttcggagttc gacaacaacc ttcaacttat taaaacaaat agcataaaat
     6301 tctctgaaga aacattactc gaaacggttg acaacttatt actaaagaac attaaaggcg
     6361 ttgacttttt aaaaattcag caaatggatg tgatgagtaa tggcaatatt atgctactta
     6421 ttgaaaagca atttgcctcc tacaccaaac ctgctgatgt gaatggtaaa aaagttgcat
     6481 caatcggtag ggagacttct gcaaacgaac tgcttttaat ttgtaccgat atgcaagata
     6541 aaaatatgca gactgttatt aaaagaaaag taagtgcaac agaaaatttc gagtacgttt
     6601 gtaatgttaa aggtattcca cataaagacg actactacct ttatttttta gatggaagaa
     6661 atccggaaga gtatcagatg acgcaatcta tttacaattc tcagctcagg agaatttaca
     6721 ctaaggaaat ggatttggct tcggcaaaaa aacttctgcc tgatatgtca tctattacac
     6781 gcctagataa gaacaggttt atcctgtatg caagactgtt aaagaaattt tctactgcag
     6841 ttttagactt caaataaaaa tgtttagtaa taggcaataa aaaaccgctc tcaataatga
     6901 gaacggtttt caattttcgg ccctaaccgt gggatattac gcagcattgc aagctaaata
     6961 ctgcaccacc ttggtgggtt aaaattattt tgcagattcg tctgacttag tgtcggtctt
     7021 aacatcagat gcaccggcac cagctttttt atgtgcgcag caacctgctt ttgcttgttt
     7081 gtcggcacat gatgcagctg aagcttctgc tttgtcagca caacattttt tctttttgct
     7141 tttctttttg cagcaatctt tgtctttttc tgccttacca tcacatggca tgtcatgatt
     7201 tgctacaata ctgctttcag ttacctgaag atcagtaact gtgtttgctt tgttgctgtt
     7261 tgcataagcg ttaacaatca ggaatagtcc gagtacgctt aaaactgaaa gtgctttttt
     7321 catttttatt tataattttc tgctaaaata gtattttaag ttctataata caactgacca
     7381 aagtcactgt tgcgactttt tcattaaata accatctgtt aaatcacttt tctgcctacc
     7441 agtcagagtg tttttttaaa acaccatcct tcaacaccgg aactacaaga gtttgattag
     7501 gtgtcaggca atacaatacg tcttcatcaa ttttaagcct ttccaatcgt gtacgatgtg
     7561 aagagtcttt taaaaaatcg tacatgtcat gctttgcaag gttataaaga ttcatggctg
     7621 caatagcaga atcacgatgc tctgaaagtt gaaaatgtgg aagaagtttt tgcgataatg
     7681 cacctgcaaa cagtgtgtct tcaagattaa aagtattttt ccatcctgca caaaggcaaa
     7741 gcacatcttt attctgctca ataagccatg cagccagtac atcaagattc agaaatgaac
     7801 ctgcaacaat tcctgctgag ccattggcaa tgttaattgc cttagtacca ttggtagtag
     7861 ttagtgcaat atcacgacca attattttgt cttccatata actgaaaggc gaattaccaa
     7921 ggtcaaatcc ttctaccatt gagccctgcc gctctgctcc tatcagatat cctttttctt
     7981 tatattgaaa actttcttcc acagtcatta caggtacaat ggagcgtgca ccatattgca
     8041 acgccacaca aatactactc gtggcacgca aaatatcaat ggcaactact atagattcct
     8101 ttgtgttgta ctgatagaaa agtgccggtg ttaaacaaac ttcaactata ggtttgcctg
     8161 cttcagacat ggattatttt ttaataaacg gaatcttgac aactgcggct ttaatcagtt
     8221 tttctctgat tttaataaaa atctctgttc cttcggcaga ttgatctttc gatacatagc
     8281 ccattccgat ggctttgttt aatgttggcg actgtgttcc tgatgtaaca cgaccaatct
     8341 tctgacctga tgcattacaa atttcgtaat cgtgtcttgg aatt
//
LOCUS       DAOOOL010000107         8373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_824_length_8373_cov_48.172758, whole genome shotgun sequence.
ACCESSION   DAOOOL010000107 DAOOOL010000000
VERSION     DAOOOL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_8373_cov_48.172758"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="PKU82_08910"
     CDS             complement(<1..1833)
                     /locus_tag="PKU82_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOZ83032.1"
                     /translation="MKKQKEFQPSLQEVNASVDTSGKRTLFKKIFAFFGPAYLVSVGY
                     MDPGNWATDIAGGSKFGYQLLWVLLMSNLIALLLQSLSARLGIVRNRDLAQASRETYP
                     HLVNICLYVLAEIAIAACDLAEVLGMAIGLQLLFGISLATGVMITVLDTFLLLFLINL
                     GIRKMEAFIISLVAIIGFSFLAEIFFAKPNMAEVASGLIPTALSNEALYIAIGIIGAT
                     VMPHNLYLHSSLVQTRKIEKSSKSIWRAIRFNIADSAIALNMAFFVNAAILVLAAAVF
                     HKSGLFEIAEIQDAHQLLAPMLGNNLAPILFAVALIAAGQSSTITGTLAGQIVMEGYI
                     NLRIQPWVRRLVTRLIAIIPAFITIHVFGERSTGELLVLSQVILSLQLGFAVIPLIHF
                     VSDKNKMGEFAIGPIIKTLSWICAIIIVGLNIKLVAEAMSDVFYSDTLLLWKILLALV
                     CLGIGWLLLMTVFYPLISKASFRSKVFHDEPSQISTPESIKYKRIAIALDFSSSDKKA
                     IENALSVGGLNTHYLLIHAVESAGALTFGKEIEDMESEHDKQHLEHYIKTLKGMGYFA
                     EYQIAHGNPGNVIPEIVNDFDAGLIVMGQHGHKGLKDFLLGTTID"
     gene            complement(1830..2501)
                     /locus_tag="PKU82_08915"
     CDS             complement(1830..2501)
                     /locus_tag="PKU82_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOZ83033.1"
                     /translation="MYYQLSPTEQNYIKQIYALSDGNHQNVLTSDLAAAIKIKAATAT
                     DMLSRLSDKKLVDYEKYYGARLTDKGKQVALNIIRKHRLWEVFLSEKLGFSWDEVHEM
                     AEELEHATSDELGNRLEQFLGNPKTDPHGDPIPNRKGEIEKQKTIRLSEAEQNHTVEI
                     RSVEDQNTALLKFLSKHKLTPGARLLVKEINQYDSSLEVKVNNKKVLQLSRDVAENLW
                     IIRVK"
     gene            2617..3648
                     /locus_tag="PKU82_08920"
     CDS             2617..3648
                     /locus_tag="PKU82_08920"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOZ83034.1"
                     /translation="MYKLLIKPLLFLFPPEQAHHLVFSMLKMAFKIPGVKTFIKSLYS
                     TGKQGTQVAIGNLKFNSRVGLAAGFDKDAYCFDELAAFGFGFIEIGTVTPRPQPGNPK
                     PRLFRLPADEALINRMGFNNEGVDAVVERLKNRKSDVIIGGNIGKNKDTANEDALSDY
                     LICFEKLFDVVDYFVINVSSPNTPGLRALQEKEPLTKLLNGIQQKNKSYRNPKPVFLK
                     IAPDLTYEQIDEVAVLVEETNLNGLIVSNTTISRENLKADADEVKNLGAGGLSGKPLQ
                     SRSTEIIRYLTQSKKVKFDIIGVGGIHSASDAIEKIEAGAKLVQLFTGFIYEGPGLVK
                     KINRAMIKK"
     gene            3781..4596
                     /locus_tag="PKU82_08925"
     CDS             3781..4596
                     /locus_tag="PKU82_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOZ83035.1"
                     /translation="MQGMHDFVPTDLKIEYINKLLEVGFDTIDFGSFVSSKAIPQMRD
                     TAEVLAGLNKSTTKLLAIIANVRGAEDAVKHQAITYLGYPFSISETFQKRNTNATIQE
                     SLANLNTIQQLTQNAGKKLVVYISMGFGNPYGEEWNVDYAAGWIDKIQSMGVEIISLA
                     DTVGVAKEADISYLFEGVIQKFPKVEFGAHLHTTPGNWQNKLAAAYDAGCRRFDTAIR
                     GFGGCPMATDKLTGNLATENAVQYLSGKLKNLTIDIQKLNDCILFSNRIFNEH"
     gene            4586..>5828
                     /locus_tag="PKU82_08930"
     CDS             4586..>5828
                     /locus_tag="PKU82_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83036.1"
                     /translation="MNIKKAVSFAKKLFALSVILGLASVSLALAQKGSLELLGNVKQN
                     GKGLEGAEISIMKGNEQVDNILSGNGGKFIINLDLDAVFTLVFSKVGCVTKSIVFDTR
                     VPEPVKNQLLSNRKFGIELFKVPEGVEQPKELAKPVAKWSWNDAYDDFDYDLEYSNAR
                     KQENEVVKKQLEEQALVQEKQKKDSLAKARLDSINQAKADEQARLAELAAKKAEEAKQ
                     KKIEEDRLAAISAAKKQEEDSLRAAQLKADAIERARLAALEKARQDSIAKANALAEQL
                     AREQAAEQKRLAALEKARQDSIAKADAAARTQLAALEKARQDSIAKADAAAKAAEAAR
                     LAALEKERKDSLTKAKAEEERLAKEQAKEQARLAALEKARQDSIAKADAAAKAAEEAR
                     LAALEKERKDSLAKAKAEEERL"
     assembly_gap    5829..5928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5929..6834
                     /locus_tag="PKU82_08935"
     CDS             <5929..6834
                     /locus_tag="PKU82_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83037.1"
                     /translation="EKERKDSLAKAKAEEERLAREQAKEQARLAALEKARQDSIAKVE
                     AAAKAAEDARLAALEKARQDSIAKADAAAKAAETARLAALEKARQDSIAKAEAAAKVA
                     EVARLAALEKARKDSIAKAEADAKEQEKLRLVALEKARQDSIAQAKILADKKAKEEAA
                     ERARLAALEKARQDSLAKAQALADAEAKRKAQEEAEAQRLREIEAQKQALARMNEQKD
                     KKQNTASLPVFIDKDYAEGLTEETIDESNRKIYRTIIKKSNTTDVFSKVVYNWGGIYF
                     FKNTTSMSESNYNNELKKYKDQLGK"
     gene            7040..7318
                     /locus_tag="PKU82_08940"
     CDS             7040..7318
                     /locus_tag="PKU82_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83038.1"
                     /translation="MKKLLLVFALGLGLTFAQAQTVATTNNKATTTHAAKATSVKSEK
                     STAADHTKKDGTPDKRFKENKNVKAQGPKKADGTPDMRYKSNKPAAKK"
     gene            complement(7573..7917)
                     /gene="rplT"
                     /locus_tag="PKU82_08945"
     CDS             complement(7573..7917)
                     /gene="rplT"
                     /locus_tag="PKU82_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOZ83039.1"
                     /translation="MPRSVNSVASRARRKKLLNRAKGNWGARGNVLTVAKHTVEKGLQ
                     YAYRDRKTKKRNFRGLWIQRINAEVRNYDMSYSEFMGKLHKAGNQLNRKVLADLALNN
                     PDAFKAIVESVK"
     gene            complement(8002..8199)
                     /gene="rpmI"
                     /locus_tag="PKU82_08950"
     CDS             complement(8002..8199)
                     /gene="rpmI"
                     /locus_tag="PKU82_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOZ83040.1"
                     /translation="MPKMKRNSSAKKRFSVTGTGKIKRKHAFKSHILTKKSTKRKRAL
                     TNAAIVHKSDEFNVKRMLSIA"
     gene            complement(8234..>8373)
                     /locus_tag="PKU82_08955"
     CDS             complement(8234..>8373)
                     /locus_tag="PKU82_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOZ83041.1"
                     /translation="AYKEKGEIILLKFAQELEQYAKVEMLPKLEGNRMFLHLAPLAKK
                     K"
BASE COUNT         2692 a         1544 c         1672 g         2365 t
ORIGIN      
        1 gtcaatagta gttccaagta aaaaatcttt taatccttta tgcccatgct ggcccataac
       61 aattaagcct gcatcaaaat catttacaat ttctggaata acatttcccg ggttgccatg
      121 tgcaatctga tactcggcaa agtaacccat gcctttcaac gttttaatgt aatgttctaa
      181 atgttgtttg tcatgttcac tctccatgtc ttcaatttct tttccgaagg ttaatgcccc
      241 tgcactttcc acagcatgaa tcaacaaata gtgagtatta agtccaccaa cacttaatgc
      301 attttcaatc gctttcttat cgcttgaact aaaatccaac gcaatagcaa tgcgtttgta
      361 ttttatactt tcaggagtag aaatctgact gggttcgtca tgaaatactt ttgacctgaa
      421 agatgctttg cttattaaag gatagaacac tgtcatcaat agcaaccatc cgattcctaa
      481 acaaactaaa gccaatagta ttttccagag taatagtgtg tcgctataaa atacatcact
      541 cattgcttct gcaaccaact taatgtttag tcctacaata attatagcac atatccacga
      601 cagcgttttt ataatagggc caatagcaaa ttcgcccatt ttatttttat cgcttacaaa
      661 atgaatgagt ggaattacag caaagcctaa ctgtaaactt aaaattacct gacttaaaac
      721 taacagctca ccggtgcttc tttcgccaaa aacatgaatg gtgataaatg caggaataat
      781 ggcaataagg cgtgtaacta atctgcgcac ccagggttga atacgaaggt ttatatatcc
      841 ttccatcaca atctgacctg ctaatgttcc ggtgatggta gaactttgtc ctgctgctat
      901 caatgctaca gcaaaaagaa taggagcaag gttgtttccc agcatgggcg caagcaactg
      961 atgtgcatcc tgaatttctg caatttcaaa aagtcctgat ttatgaaaca ctgctgctgc
     1021 aagtactaaa atagctgcat tgacaaagaa tgccatgttc aatgcaatag cactgtcagc
     1081 aatattaaat cgaatagctc gccaaattga cttgctgctt ttttcaattt ttcttgtctg
     1141 caccaatgac gaatgcagat aaagattgtg aggcataaca gtggcaccta taattccaat
     1201 agctatatac aaagcttcgt tactcaatgc tgttgggatt aatcctgaag ctacttctgc
     1261 catattgggc ttggcaaaaa atatttcagc caaaaaagaa aagccaatta ttgcaactaa
     1321 agaaattata aaggcctcca tttttctgat gcctaaattg atgagaaaca aaagcagaaa
     1381 agtatccaat acggttatca ttacacctgt tgcaagcgaa attccaaaaa gtagttgaag
     1441 cccaatggcc atacctaaca cctctgcaag gtcgcaggct gcaatagcta tttctgctag
     1501 cacatagagg caaatattta ccaaatgcgg ataggtttct cttgatgcct gcgcaaggtc
     1561 gcggttgcgc acaatgccta aacgggcact caggctttgc agcagtaatg caataagatt
     1621 actcataagt agcacccaaa gaagttgata accaaacttg cttcctccgg caatatctgt
     1681 tgcccagtta ccggggtcca tatagccaac actgactaaa tatgctggcc cgaagaaggc
     1741 gaaaatcttt ttaaacagtg tgcgttttcc tgaagtgtca accgatgcat ttacctcctg
     1801 taatgagggt tgaaattctt tttgcttttt cattttactc taataatcca taaattttct
     1861 gcaacatcac gactgagttg aagcactttt ttattgttga cttttacttc taaagaactg
     1921 tcatactgat taatttcttt tacaagcaac cttgcaccgg gtgttaattt atgctttgat
     1981 aaaaatttca ataaggcagt gttctgatct tctacagaac gaatttcaac agtgtggttt
     2041 tgctcggctt ccgaaagacg aatagttttt tgtttttcta tttcgccttt acggttagga
     2101 atagggtcgc catgtgggtc ggttttcgga ttacccaaaa attgttctaa gcgattacct
     2161 aattcatcgc tggtggcatg ctctaactct tccgccattt catgcacttc gtcccaactg
     2221 aaacctaatt tctctgataa aaaaacttcc cacagacgat gctttctgat aatgtttaat
     2281 gccacctgct ttcctttgtc tgtcagcctg gcaccataat atttttcata gtcaaccaat
     2341 ttcttgtctg ataacctcga taacatatct gtagctgtgg ctgctttaat cttgatggct
     2401 gctgcaagat cagaggtcag cacattttga tggtttccat cagaaagtgc ataaatctgc
     2461 tttatatagt tctgctcagt aggcgatagt tgataataca tcctgtaaaa atttagacaa
     2521 atgtagtaat aaaatttaga ttcgtctaaa taaaatgaac atacttttat aaaatcattc
     2581 tgaccgtttc tgatggttta gttttaattt tgcagcatgt ataaactttt aatcaaacct
     2641 ttattgtttt tatttcctcc tgaacaggca catcatcttg tttttagcat gttgaagatg
     2701 gcgtttaaaa ttcccggagt taagactttc ataaaatctt tgtattctac aggtaaacaa
     2761 gggacacagg ttgcaatagg gaatcttaaa tttaattcgc gggtagggct tgcagcaggc
     2821 tttgataaag atgcttattg ttttgatgaa ctagcggctt ttggttttgg ttttattgaa
     2881 attggaacag taacaccacg tccgcagccg ggcaatccta agccccgact gttcagactt
     2941 cctgccgatg aggcactcat taacagaatg ggatttaaca atgaaggtgt ggatgctgtt
     3001 gttgaacgac tcaaaaaccg gaagtcagat gttataattg gtggaaacat tggaaaaaac
     3061 aaagacacag ccaatgaaga tgcgcttagc gattatctga tttgttttga aaaactgttt
     3121 gatgtggtgg attattttgt gataaatgtg agttcgccca acactcctgg tttacgtgca
     3181 ttacaggaaa aagaaccatt gacaaaactt ctgaacggca ttcagcaaaa aaataaaagc
     3241 tatagaaatc caaaacctgt ctttttaaaa atagcccctg accttactta tgagcagata
     3301 gatgaagttg cagttttagt tgaagagact aatctgaatg gcttgatagt cagtaatact
     3361 acaattagta gagaaaactt aaaagctgat gctgacgaag tgaaaaactt gggtgcagga
     3421 ggtttaagcg gaaagccttt gcaaagccgt tcaacagaaa taatccgcta tctcactcag
     3481 tcaaaaaaag taaaattcga cattattggt gtgggaggta ttcattcagc atctgatgcg
     3541 attgagaaaa tagaggctgg tgcaaagttg gtgcagctat tcacaggatt tatttatgaa
     3601 ggaccgggat tggttaaaaa aatcaatcgt gccatgataa aaaaatgaaa aaacagtttg
     3661 ttgattactt acattcatag cagaagaaac acttcattta cttttttata tttgaaacgt
     3721 cattaaaacc aaatcacctt gccggaaaag ttgaaaataa ttgaatgtcc gcgtgatgcc
     3781 atgcagggca tgcatgattt tgttcctacg gacttaaaaa tagagtacat aaacaagttg
     3841 cttgaagttg gtttcgatac tattgacttc ggtagttttg tttcgtctaa agccattcca
     3901 caaatgcgcg atacggcaga agtgttggca ggacttaata aaagcaccac taagctactt
     3961 gccataattg ccaatgtacg cggtgctgaa gatgcagtaa agcatcaggc aataacttat
     4021 ctgggttatc cgttttctat ttccgagact tttcagaaaa gaaacacaaa tgccaccatt
     4081 caggagtcgt tagctaactt aaacactatt cagcagttga cacaaaatgc aggcaagaag
     4141 ttggtcgttt acatttccat gggttttggt aatccctatg gtgaagaatg gaatgttgac
     4201 tacgctgctg gatggattga taaaattcag tctatgggag tagaaattat ttctttggcc
     4261 gatacggttg gtgtagctaa agaagcagat atttcatacc tgtttgaagg cgttatccaa
     4321 aaattcccta aggtagaatt cggagcgcat ttgcatacca cccccggcaa ctggcaaaac
     4381 aagctggcag cggcttatga tgcaggttgc aggcgttttg atacggcaat tcggggtttt
     4441 ggcggctgtc cgatggctac tgacaaatta accggaaacc ttgcaaccga aaatgcagta
     4501 caataccttt caggtaaatt aaaaaattta acaatagata ttcaaaaact gaatgattgc
     4561 atcttattct caaaccgcat cttcaatgaa cattaaaaaa gcagtttcat tcgcaaaaaa
     4621 gttatttgcg ctttctgtaa tactcggttt agcatccgtt agccttgctc ttgcccaaaa
     4681 aggcagcctt gaacttttag gaaacgttaa acaaaacggc aaaggacttg aaggtgctga
     4741 aatcagcatt atgaaaggta atgagcaggt ggacaatatt ctttccggaa atggtggaaa
     4801 gtttatcatc aaccttgacc ttgatgctgt ttttacactt gtattttcaa aagtaggatg
     4861 tgttaccaag tcaattgtat ttgatactcg tgtaccggag cctgtaaaaa atcagttgtt
     4921 atcaaacaga aaattcggaa tagaattatt taaagtgcct gaaggcgttg agcaacctaa
     4981 ggagttggct aaaccagttg ccaaatggag ttggaacgat gcttatgacg attttgatta
     5041 tgatttggag tatagcaatg cccgtaaaca agaaaacgaa gttgttaaga aacagttgga
     5101 agagcaggca ttagttcagg aaaagcagaa gaaagattcg ttggcaaaag cacgtttaga
     5161 ttccatcaat caggctaagg ctgatgaaca ggcgcgactg gcagagttgg ctgcaaagaa
     5221 ggcagaagaa gccaaacaga aaaaaataga agaggatagg ttggcagcaa tcagtgcagc
     5281 taaaaagcag gaggaagaca gcttgcgtgc agcacaactt aaagcagatg caatagagcg
     5341 cgcacgatta gcagcattag aaaaagcacg ccaggatagt atagcaaaag caaatgccct
     5401 tgccgaacag ttggctcgtg aacaggcagc agaacaaaag cgtttagctg cattagaaaa
     5461 ggccaggcaa gacagtattg ccaaagcaga tgcagcagca agaactcaac ttgcagcgtt
     5521 agaaaaagca cgacaagata gtattgccaa agcagatgca gcagctaagg cagcagaagc
     5581 ggctcgattg gcagcattgg aaaaagaacg taaagattca ttaactaaag ctaaagccga
     5641 agaagagaga ttggcaaaag aacaagctaa ggagcaagcc cgtttagcgg cattagaaaa
     5701 ggcacgtcag gatagcattg ccaaagccga tgcagcagca aaggctgcag aagaagcacg
     5761 tttggcagca ttagaaaaag aacgtaaaga ttctcttgct aaagctaaag ccgaagagga
     5821 acgcttggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaaagaacgt
     5941 aaagattctc ttgctaaagc taaagccgaa gaggaacgct tggcgcgcga gcaagcaaaa
     6001 gagcaggcac gtttagcagc attagaaaaa gcaagacaag atagcattgc caaagttgag
     6061 gcagcagcaa aggcagcaga agatgcacgt ttggcagcat tagaaaaagc ccgtcaggat
     6121 agcattgcta aagcagatgc agcagcaaag gcagcggaaa cagcacgctt agcagcatta
     6181 gaaaaagccc gtcaggatag tattgccaag gcagaggcag cagctaaagt tgcagaagtc
     6241 gcacggttag cagcattgga aaaagcaaga aaagacagca tagccaaagc agaagctgat
     6301 gctaaagaac aagagaaact tcgtttggtt gcattagaaa aagcacgtca ggattcgata
     6361 gcacaagcaa aaatattagc tgataaaaaa gcaaaagaag aggccgcaga acgtgctcgt
     6421 ttagcagctt tagaaaaagc gcgtcaggat agtttggcta aagcacaggc attggctgac
     6481 gctgaagcca aacgtaaagc tcaggaagaa gcagaagcac aacgtttgcg tgagattgaa
     6541 gcgcagaaac aagctcttgc acgcatgaat gaacagaaag ataaaaaaca gaatacagct
     6601 tcattacctg tatttattga caaagattat gcagaaggac ttacagagga aactatagat
     6661 gagtccaacc gcaaaatata tcgcaccatc atcaagaaat ctaatactac agatgtgttt
     6721 tcaaaagtag tttataactg gggtggaatt tatttcttta aaaatacgac cagcatgtca
     6781 gagtcgaact acaataacga actgaaaaag tataaagacc agttgggaaa atagcatttt
     6841 tctgtttttt tgatttacac attaaagcat tgtctattta attaaaaact gttatagtct
     6901 aacaatggtg ttattttttt atgcaagagt gatagtcgga aattaatatc acttcagaaa
     6961 aaatacatgc gttttaaaaa taaattcatg tggtggtcat ctgtgccggt tcatatttgt
     7021 tgcataaatt tcaaaaaaaa tgaaaaagtt attattagta tttgcattgg gattaggttt
     7081 aacctttgcc caggcacaaa ctgttgctac taccaataac aaagcaacaa caactcatgc
     7141 tgcaaaagct acttcagtaa aatcagaaaa gagtactgct gcagatcaca caaaaaagga
     7201 tggtaccccc gacaagcgct ttaaagagaa caaaaatgta aaagcacaag ggccaaaaaa
     7261 agcagatggc acacctgaca tgcgttacaa atcaaataag cctgctgcaa aaaaataaag
     7321 taagcgtttg aattatttta aaggctgttg cacgttatgc aacagctttt ttgtttttat
     7381 tattttgata caacttataa caatgttgat gatagacgaa ataagaacat aatttaaact
     7441 gaagaatttt tcaatttgaa tgctaacaaa atcaccattc ttgttaatac tttataagtt
     7501 gtgtaaatta aattttaccc acaaaaaaaa gctgcccttt cgaggcagct tttacaaaaa
     7561 tgtattgtca atttatttta cagactcaac aattgcttta aaagcatcag gattattaag
     7621 tgcaaggtcg gcaagaactt tgcggttaag ttggttacct gctttatgca gtttacccat
     7681 aaattcgctg taagacatat cgtaatttct tacctctgca ttaatacgct gaatccataa
     7741 gccgcggaag ttgcgtttct tggttttacg atcgcggtat gcatattgca aacctttttc
     7801 tactgtgtgt ttggcaacgg ttaatacgtt acctcttgcg ccccaatttc ctttggcgcg
     7861 gttgagcagt tttttgcgtc ttgctcttga tgctactgaa tttaccgaac gtggcatgtg
     7921 ttttgaattt ttggcgttag cgctccgctt tgcggaatct ttatgctaat tacccggtta
     7981 aacttaattg aatattattt tttatgctat tgaaagcatt ctttttacgt taaactcgtc
     8041 agacttgtgt acgatggcag cattggttaa tgcacgctta cgcttcgttg acttcttagt
     8101 caggatatgg cttttgaaag catgctttct ttttatttta cctgtgcctg taacagagaa
     8161 tctttttttg gcactggagt ttcttttcat tttcggcata acgcttctaa tttttatttg
     8221 tttattattt tatttacttc ttctttgcaa gtggtgccag gtgcagaaac atgcggttgc
     8281 cttctaattt cggaagcatt tcaacctttg catattgctc caattcctgt gcaaacttca
     8341 ggaggataat ttctcccttt tctttataag cta
//
LOCUS       DAOOOL010000108         8249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_849_length_8249_cov_51.615084, whole genome shotgun sequence.
ACCESSION   DAOOOL010000108 DAOOOL010000000
VERSION     DAOOOL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_849_length_8249_cov_51.615084"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKU82_08960"
     CDS             <1..304
                     /locus_tag="PKU82_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83042.1"
                     /translation="HTDIVPGVLNYLHYPDKFKFFGNSIFDTTVSSCFAVSFLDGTYQ
                     LITNNSVTTTDFDTEFHFVLRDAKTVNDSIKASNTLKVFVSLYNRSMIHNKLTEDE"
     gene            318..1169
                     /locus_tag="PKU82_08965"
     CDS             318..1169
                     /locus_tag="PKU82_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOZ83043.1"
                     /translation="MFILNPKAGHGLNEDIIVVAGKILSKNFGECIFKKTEFVGHATT
                     LAAEAVKDKIDLVVCVDGDGTVNETAQALKGSETSLAIVPTGSGNGLARHLRIPMNYK
                     RAIGLINDGKKFAIDTFLVNDRFAVNLAGVGFDALVAHRFAALKVRGLQSYIKTVIRS
                     ISDFEEMKFTDVSGQQQKAWLMCIANSSQFGNNARIAPAAQVNDGMLDVVMIKRPSVL
                     KMIPFAVKMFNGKLETSNSYRCIRVKKYSVDFEKEQPMHIDGEPAGMASSVEVQLIPQ
                     SLNIIIP"
     gene            1171..1515
                     /locus_tag="PKU82_08970"
     CDS             1171..1515
                     /locus_tag="PKU82_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOZ83044.1"
                     /translation="MSKKKNESSGIVYSTNPDFKYNNDNNTESETPAPAQQDLRVLRD
                     SKQRGGKTVTLITGFVGKEEDIEKLTKMLKTKCGTGGTFKDGEIIIQGDQREKVFDLL
                     LAAGYKVKKAGG"
     gene            complement(1880..3121)
                     /locus_tag="PKU82_08975"
     CDS             complement(1880..3121)
                     /locus_tag="PKU82_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ83045.1"
                     /translation="MKFTILTQYYPPETGAPQNRLSDLALQLKNAGHDVTILTAMPNY
                     PQNEIHANYKGRYFISESLNGISILRSWIYVGKSRSVIPRLFNYFSFVISSFIWGIIK
                     LKKQDVIICESPPLFLGISGLLLSVFKKSKLVFNISDLWPESAEKLGVITNKTLLKLA
                     ERLELFLYRKSFLITGQTQGIVKNIGDRTNLSNLHWLPNGISDDVVNLNLNKDDCRKK
                     LNLADDSFIFSYTGVIGHAQGLEIIIHAAEKFVSKNVTIVFCGDGPELESLKALADKS
                     GFNHFVFTGAVDKKVALQVVAASDAAIIPLKRLDLFKGAIPSKIFENCALGKPILLGV
                     DGEAKELFVNQAQAGIYFEPENINELSTAMEKLFENNILCTSLGQNGKQYVLSKFSRK
                     QIAINFLNVLEQKLKDSTSTQ"
     gene            complement(3133..4212)
                     /locus_tag="PKU82_08980"
     CDS             complement(3133..4212)
                     /locus_tag="PKU82_08980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ83046.1"
                     /translation="MKILLLANADSIHTKKWCIALAERGFEIAVFSMSPTDTLWINEK
                     NIKLFVNNRSKKGEHHLSKITYLAALPALSKVIRQFKPDIVHAHYATSYGLLGYLSKF
                     KPFYISVWGSDIIDFPRKSFFHKLLLQKILKSSDKLMCSSQYLKRQLNDLVSKEVKVI
                     PFGVDTKLFRPTTSDKRFSGITIGIIKSLEPVYNIPLVIEALNILKNKYKIDNCKLLI
                     VGGGSLESKLKQQVNNLGLGNDVIFTGKVEQNKVVEYHNAIDIFINVSVYESLGVSVL
                     EASACEKPVIATNVGGLCEVVEDNKTGFLIDEVTPEKLAEKILFLINHPEKRNEMGVN
                     GRIYVIKYFEWEQSINQMVNEYLNN"
     gene            complement(4216..5520)
                     /locus_tag="PKU82_08985"
     CDS             complement(4216..5520)
                     /locus_tag="PKU82_08985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOZ83047.1"
                     /translation="MTVTPNSIPANNTYQLLQKILCFCLAITSFMAVPYSKSIAFDWF
                     IQLPGSYKISLFNLVEIISFSTAFFIIRLRVLAGIERRILIALLLVMATRVISLLFAK
                     QYESIQYFSILRFAEVLLGLIILTNLLQSKANRNIFIAGLACGLLIECFLGLNMVIQT
                     GGHLRGIFFGIPSYQMMVLFILFLFLLTKSQNLIILIFAINILLTGIMATQTRAALIQ
                     LAVGMIVVIIIAYKQGFFKRFAITLVVVTIVHSITLYSMQSVISAAKERFEDAIKEGK
                     IHLEEDEHSVGGGTIQYRLYLWDKSIGAFLSHPVTGIGSGSFPRQLDSLPQKFGVQLE
                     RNDKNLPLSTHNTFLGVLAETGIVGITAYFFWLSCIIILIIKYYRSSSHRSFETAVAI
                     MLGIFIFSDFWSQQSFLPNMTYLTAYLLASLRSENYNPLPSS"
     gene            complement(5569..6843)
                     /locus_tag="PKU82_08990"
     CDS             complement(5569..6843)
                     /locus_tag="PKU82_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOZ83048.1"
                     /translation="MAATEHKEYYRNLITLVTGSAFAQLISLALSPVFSRIYTPHEFG
                     LLAVFMSILSALSVIVCLRYEFAIIPARTGKDAFHLLELCFYSTLLITALTLVGVTIF
                     NLFFSTNAVLSKWIWFLPFMVLTNGSYQAMLSWANRHKSYKVISRYRIINSVVTNTII
                     LGAGLAGFISMGLLAGFVTGGVVASIMLYFNIQKQSEPFTLGFDLTALKNVAKKYEAF
                     PKVNLWQSIADMLLINGVIYFLTAFFSPAVVGLYGLTMRVLQAPFNLIGYSMGQVFIQ
                     DAAHQYNEGKNIQPIIKKSIQRTAFIAIPIVLPLILAGPQLFAFVFGENWRQSGEYAQ
                     ILAPWFFLDFIRTPLGQIPIIMGKQKRQFTITIAGNILMVISLLLGGFYFNNPLLVVT
                     LFTALQSLYLIYVIYWIYSISGVTSEIRDDSR"
     gene            complement(6863..>8249)
                     /gene="rny"
                     /locus_tag="PKU82_08995"
     CDS             complement(6863..>8249)
                     /gene="rny"
                     /locus_tag="PKU82_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOZ83049.1"
                     /translation="LEAKEKFFQLKSEHEKMISEKDKKISDSENRVKQKERELNQKLE
                     QNSRKHQEYEALQTNLKGQMQIVEARKQEVDKSLQKHVQALEKIAGISQDEAKTQLME
                     TLKAEARTQAQSYIKEIMDEAKLTANKEAKRIIIQSIQRVATEHAVENSVTVFNLEND
                     DVKGRVIGREGRNIRALEAATGIEIIVDDTPEAIILSGFDPVRRELARLALHQLVSDG
                     RIHPARIEEVVNKVRKQLEEEIIETGKRTCIDLGIHGLAPELIRMVGRMKYRSSYGQN
                     LLQHSREVANLCAIMASELGLNAKLAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKN
                     KENAEVCNAIGAHHDEIEMTSLISPIIQVCDAISGARPGARREIVEQYIKRLKDLEAL
                     ALSYPGVEKSFAIQAGRELRVIVESEKVSDQQADTLSFDLAQRIQTEMTYPGQVKVTV
                     IREKRAVNYAK"
BASE COUNT         2590 a         1466 c         1596 g         2597 t
ORIGIN      
        1 gcatacagat atagttcccg gagtattaaa ttatctgcat tatcctgata aatttaaatt
       61 tttcggcaac agtatttttg atacaacagt cagcagttgc tttgctgtta gttttcttga
      121 tggtacttat cagttgatta caaacaacag cgttacaact acagactttg atacggagtt
      181 tcattttgta ctgcgtgatg caaaaacagt gaacgattca attaaagctt caaatacatt
      241 aaaggttttt gttagtttgt ataacagaag tatgattcac aataaactta cggaggatga
      301 atagcagcaa gcgcgtaatg tttattctga atcctaaagc cgggcatgga ctaaacgagg
      361 atattattgt tgttgcgggt aaaattctca gtaaaaattt tggcgaatgt atttttaaga
      421 agactgaatt tgtaggacat gcaactacac ttgcggcaga agcagtaaaa gataaaattg
      481 atttagttgt ttgtgttgat ggcgatggta cagttaatga aacagcacag gctttgaaag
      541 gtagtgaaac atcattagct atagtaccaa caggttcggg aaatggactt gcacgtcatt
      601 tgcgcatacc gatgaattac aaaagagcta ttgggttaat taatgatgga aagaagtttg
      661 caattgatac ttttttagtt aatgacaggt ttgccgtaaa ccttgcagga gtaggttttg
      721 atgcacttgt tgcacatcgt tttgcagcgt taaaagttag agggttacaa tcgtatatta
      781 aaactgtaat acgcagtatt tctgattttg aagagatgaa atttactgat gtatcagggc
      841 agcaacaaaa agcatggttg atgtgtattg ccaacagttc gcaatttgga aataatgcac
      901 gcattgcacc ggcagcacag gtaaacgatg gtatgttgga cgttgtgatg ataaagcgtc
      961 catctgtttt gaagatgatt ccatttgctg taaaaatgtt taatggtaaa ttggaaacat
     1021 caaatagtta tagatgtatc agagtaaaaa aatattctgt tgattttgaa aaggaacaac
     1081 ccatgcatat tgatggcgag cctgccggaa tggcttcatc ggttgaagtg caattaattc
     1141 cacaatcttt aaatattata attccataaa atgtcgaaga agaaaaacga aagctcagga
     1201 attgtttata gtaccaatcc tgattttaaa tacaacaacg ataacaatac agagtctgaa
     1261 acaccggcac cggcacagca ggatttaaga gtgctgcgag acagcaaaca acgtggtggt
     1321 aaaacggtaa cgctgataac gggatttgtg gggaaagaag aagatattga gaaactcaca
     1381 aagatgttaa agactaagtg tggcacagga ggaactttta aggatggtga aataattatt
     1441 cagggtgatc aaagagaaaa agtttttgat ttattgcttg cagcaggata caaggttaag
     1501 aaggcaggag gatagcataa aaaaagccgt cagcaggctg acggcttttt ttgaggtcga
     1561 gagtagattt gaactactgt aaaaagtctt gcggactttt gcctatactc tcggccactc
     1621 gaccttatag atgttgaatt aatttcaacg aaaacaaaga aataaagttt catcaattat
     1681 acaaaatact tttgggtgaa tagccctttt tatttggtaa acggaatata ccgtgatttc
     1741 acctctgaaa attgttaatt tcttagtctg atttttacaa cgattctact caaaattaag
     1801 tattgtttca gagaaaaatt attttatgga aggaggcttg taatgttgtg tatgttctgt
     1861 tgaagagaga aataaaatct tattgagttg aagtggaatc tttgagtttt tgttccaata
     1921 cattcagaaa attgattgca atttgttttc tcgaaaattt gctcaacaca tattgttttc
     1981 cgttttgtcc taatgaggtg cagagtatat tattttcaaa cagtttttcc atggcagtgg
     2041 atagctcatt tatattttcc ggttcaaaat aaattcctgc ctgtgcctga ttgacaaata
     2101 attcttttgc ttcgccatca acacctaata aaataggttt gccaagtgcg cagttttcaa
     2161 aaattttaga tgggatagct cctttaaaaa gatcaagacg tttgagaggg ataattgctg
     2221 catcagaagc ggctactact tgaagtgcaa cttttttgtc aactgcaccg gtaaaaacaa
     2281 aatgattaaa acctgactta tctgctaatg ctttcaaact ttccaattcg gggccatcac
     2341 cacaaaaaac aattgtcaca tttttagaaa caaatttttc tgctgcgtga atgatgattt
     2401 ccaatccttg tgcatgccct atcacaccgg tatagctaaa gataaaacta tcatcagcca
     2461 gattcaattt cttacggcaa tcgtctttgt tgagatttaa attaaccaca tcatcagaaa
     2521 tgccattagg caaccaatgt aggttagaaa gattagttct gtcaccaata tttttaacta
     2581 taccctgcgt ttgaccggta atgagaaaag attttctgta aagaaaaagt tcgagtcgct
     2641 cagcaagttt caaaagtgtt ttgtttgtga tgacacctaa tttctctgcc gactcaggcc
     2701 ataggtcgga gatattaaac acaagttttg atttcttgaa aacggaaagc agcaatcctg
     2761 aaattccaag aaagagtggg ggactttcac aaataatgac atcttgtttt ttaagtttaa
     2821 ttattcccca gatgaaagac gaaataacaa atgaaaaata attgaacagg cgcggaataa
     2881 cagagcggct tttgccgaca tatatccaac ttctgagaat tgaaatccca tttaaacttt
     2941 ctgaaataaa atagcgacct ttatagttgg catgaatttc attctgcgga taattaggca
     3001 ttgcagtaag aattgttaca tcatgtcctg cgttttttaa ctgcaatgca aggtctgaaa
     3061 ggcgattttg cggagcgcct gtttcaggag ggtaatattg agtaaggatg gtgaatttca
     3121 tggatggcaa agttaattat tgaggtattc atttaccatt tggttaatgc tttgttccca
     3181 ctcaaaatat ttgataacat agattcttcc attcacgccc atctcgtttc tcttttccgg
     3241 atggttgata aggaataata ttttttcagc aagtttttct ggagttacct catcaattaa
     3301 gaaaccggtt ttattatctt caacaacttc gcaaagccca cccacgtttg ttgcaataac
     3361 aggcttttca caagctgaag cttcgagaac agaaacgccc aggctttcat acacagagac
     3421 atttataaag atatcaatgg cattatgata ttctacaact ttattttgtt ctacctttcc
     3481 ggtaaatatg acatcattac ctaaacctaa attgtttact tgttgtttga gcttactttc
     3541 taaagagcca ccaccaacaa tcagtaattt gcaattgtca attttatatt tgtttttcag
     3601 gatatttaat gcttcaatta caagaggaat attgtaaacc ggttcaagcg acttaatgat
     3661 tccaattgta atacctgaaa atcttttatc agaagtagtt ggtctgaata attttgtgtc
     3721 aactccaaag ggtataactt taacttcttt agatactagg tcgtttaatt gacgttttag
     3781 gtattggctt gaacacatta gcttatcaga gcttttaagg attttttgca agagcagttt
     3841 atgaaaaaaa cttttacgtg gaaaatcaat aatgtcactt ccccaaactg aaatatagaa
     3901 cggtttaaat tttgaaagat agccaagcaa tccataactt gttgcataat gtgcatgtac
     3961 tatatcgggt ttaaattgtc tgattacttt acttaaagca ggtaatgctg cgagataggt
     4021 tattttagat aaatgatgtt cacctttttt ggagcgatta tttacaaaca gttttatatt
     4081 cttttcgttt atccaaagag tatcagtcgg gctcatagaa aagactgcaa tctcaaagcc
     4141 tctttcagca agtgctatgc accatttttt ggtgtggata gagtctgcat tagcaagcag
     4201 caaaatcttc atagcttatg aggatggtag tggattataa ttttcgctgc gtaatgaagc
     4261 aagtaaatag gcggtcagat atgtcatgtt gggaagaaaa gactgttgtg accaaaaatc
     4321 tgaaaaaata aatattccta acatgattgc tactgcagtt tcaaaactcc tatggcttga
     4381 agaacggtaa tattttataa tcaaaattat tatacaacta agccagaaaa aatatgctgt
     4441 aatgccaact attcctgttt ctgccaacac tcctaaaaat gtattatgtg tactcagtgg
     4501 tagattttta tcattacgtt ctaattgcac tccgaatttt tgaggtagtg aatctaactg
     4561 tcgagggaag ctgccgcttc caataccggt tacaggatgg ctaagaaagg ctccaatact
     4621 tttatcccat aaatacaatc tatattgaat ggtgccaccg ccaactgaat gttcgtcttc
     4681 ttctaagtga atttttcctt ctttgatagc atcttcaaat cgttcctttg ctgcactgat
     4741 aactgattgc atggaataaa gtgtaatgct atgaacaata gtaacaacta ctaaagttat
     4801 agcaaatctt ttaaagaaac cttgtttata ggcaattatg ataactacaa tcatgcctac
     4861 tgctaattgg attaatgctg cacgtgtttg tgtagccatt attccggtaa gtaaaatatt
     4921 aatggcaaat attaaaataa ttaaattctg tgattttgta agtaagaata aaaacagaat
     4981 gaaaagcacc atcatctgat atgatggtat tccgaagaaa atgcctctta gatgtcctcc
     5041 tgtttgaatg accatgttta agccaagaaa acattcaatc aataacccac atgccaatcc
     5101 agcaataaaa atatttctgt tggctttaga ttgcaacagg ttggtcagga taattaatcc
     5161 taataaaact tcggcaaacc tcagaatgct gaaatattga atggattcat attgttttgc
     5221 aaagagcaga gaaattactc gggttgccat tactagtaag agggcaatca gaattcttct
     5281 ttcaatacct gctaaaaccc gaagcctgat tatgaaaaaa gcagttgaga aacttattat
     5341 ttctactaag ttaaaaagac taatcttata acttccgggc agttgaataa accagtcgaa
     5401 tgcaatactt ttagagtaag gcactgccat aaaagatgta attgccaggc agaagcataa
     5461 aatcttttga agcagttgat acgtgttgtt ggccggtatg ctattgggag taacagtcat
     5521 tgtcttgaac tgcaaaaata aaaataaggt tatagcaggg gtagagaatt accttgaatc
     5581 atccctgatt tctgaggtta caccagaaat gctatagatc caataaatga cataaatgag
     5641 atatagcgat tgaagtgctg taaataaggt gaccacaagc aatggattat taaaataaaa
     5701 gccacctaac aacaaagaaa ttaccatcaa aatatttccg gcaattgtta ttgtgaattg
     5761 tcgtttttgc ttgcccatga tgataggaat ctgccccaat ggtgttctga taaagtcaag
     5821 aaaaaaccag ggagcaagta tttgagcata ctcgcctgac tgccgccaat tttcgccaaa
     5881 gacaaaagca aaaagttgtg gccctgctaa aattaatggt aacacaattg gtatagctat
     5941 gaaagcagtg cgttgaatag attttttaat aatgggctga atattttttc cttcattata
     6001 ctgatgtgct gcatcctgta taaaaacctg tcccatgctg tatcctatca ggttaaatgg
     6061 agcttgtaaa accctcatgg taagtccgta cagacctaca acagcagggc tgaaaaatgc
     6121 agtcagaaaa tagatgactc cgttaattag cagcatatcg gcaatagatt gccataaatt
     6181 tactttagga aaagcttcgt atttttttgc aacatttttt aatgcagtca aatcaaagcc
     6241 aagcgtaaat ggttcagact gcttttgaat attaaagtat agcatgatgg aggcaacaac
     6301 accgcctgta acaaagcctg caagcaatcc cattgaaata aaacctgcaa gccctgcacc
     6361 cagaataatg gtattggtaa caactgaatt aattattctg tagcgtgata ttactttgta
     6421 gcttttatgt ctgtttgccc aactgagcat tgcctgatag gaaccgtttg tcagcaccat
     6481 aaaaggcaga aaccaaatcc attttgacaa gacagcattg gttgaaaaaa acagattgaa
     6541 tatggttaca cctacaagcg ttaatgccgt aatcagcaat gttgaataaa aacaaagttc
     6601 aagcaggtga aatgcatctt ttcctgtgcg tgcaggtatg atggcaaact cataacgcag
     6661 acaaacaatt acagacaatg cacttaaaat gctcatgaaa acagctagca ggccaaactc
     6721 atgaggtgtg tatattcttg aaaataccgg tgacaatgcc aggctaatta actgcgcaaa
     6781 tgcgctaccg gtaactaaag taatcaggtt gcgatagtac tctttatgtt ctgttgccgc
     6841 catgaaaact gcctcgaggc tgttacttgg cgtagtttac cgcacgtttc tcacggataa
     6901 ccgtaacttt tacttgtccg ggataggtca tctctgtttg aattctttgt gcaagatcaa
     6961 aactcaatgt atcagcttgc tggtcgctta ctttttcgct ttcaacaata acacgcaatt
     7021 cgcgtcctgc ctgaatggca aaagattttt ctacaccggg ataggagagg gcaagtgctt
     7081 ccaaatcttt caatctcttg atgtattgct caacgatttc gcgtcttgca cccgggcgtg
     7141 cgcctgaaat ggcatcacaa acctgaatga taggactgat gaggcttgtc atttcaattt
     7201 catcgtgatg ggcaccgatg gcattgcata cttcggcatt ttctttattt ttttcggcaa
     7261 gtttcattcc cagaatagca tgtggtaatt caggctcatc atcgggtact tttccaatat
     7321 cgtgtaataa tcctgcacgt ttagcaagct tggcatttaa tcctagttcg ctggccataa
     7381 tagcacatag gttggctacc tcgcgcgagt gttgtaacaa attctgtcca taagaagagc
     7441 ggtatttcat tctgcctacc atacgaatca gttccggtgc aagaccgtga atacctaggt
     7501 caatacaggt gcgtttaccg gtttctataa tttcttcttc aagttgctta cgaactttat
     7561 tcaccacctc ctcaatacgg gcggggtgaa tacgtccgtc actcactaac tgatgcagtg
     7621 caagacgtgc taactcgcgt cttacaggat caaagccgct tagaataatt gcttccggtg
     7681 tgtcgtcaac aataatttca attccggtgg cagcttccag tgctctgatg tttcgtcctt
     7741 cgcgaccaat tacacggcct ttaacgtcat cgttctcaag attaaaaacg gtaacagaat
     7801 tttcaacggc atgctcagta gccacacgct gaatactttg aatgatgatg cgttttgctt
     7861 ctttgtttgc agtgagttta gcttcatcca taatctcttt aatataggat tgtgcctgag
     7921 tgcgtgcttc ggctttaagc gtttccatta actgcgtttt agcttcatcc tgactgatgc
     7981 ctgcaatttt ctcaagtgcc tgaacatgtt tttgaagcga tttgtcaacc tcttgtttgc
     8041 gggcttctac aatctgcatc tgacctttta agttggtctg caatgcttcg tactcctgat
     8101 gtttgcgcga attttgttca agtttctgat tcagttcgcg ttctttttgt ttgacacggt
     8161 tttctgagtc agaaattttc ttgtcttttt cggaaatcat cttttcatgc tctgacttta
     8221 gctgaaagaa tttttctttt gcttcaagc
//
LOCUS       DAOOOL010000109         8178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_861_length_8178_cov_79.647421, whole genome shotgun sequence.
ACCESSION   DAOOOL010000109 DAOOOL010000000
VERSION     DAOOOL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_8178_cov_79.647421"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1218
                     /locus_tag="PKU82_09000"
     CDS             220..1218
                     /locus_tag="PKU82_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83050.1"
                     /translation="MKKIGILYGQENSFPPAVVERINAKNISGIVAESVQVDKVVQGM
                     PMDYAVIVDRISHDVPFYRGMLKNAAICGTAVLNNPFWNSADEKFFNNCLATKIGVPV
                     PKTVLLPSNVHPPDTNENSFRNLKMPLDWDNIFKYIGFPAYMKPFAGGGWKNVYKCND
                     AADFYNKHQETGQLVMLLQEEIVFDQYFRCYCIGGKRIKIMRYEPRNPHHLRYVVDGP
                     APEQRILDVVEEYTFKLCNALGYDINTVEFAIRDGVPYAIDFCNPAPDADRHSVGDEN
                     FNWVIEAVADMCIEKALSHREGQDNLTWGAFVKHAAQGQIVNKQNMDALMESYTVK"
     gene            1263..2384
                     /locus_tag="PKU82_09005"
     CDS             1263..2384
                     /locus_tag="PKU82_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HOZ83051.1"
                     /translation="MPQFTLGVEEEFQVIDPKTRELRSHMHQIVEGGKVILKEQVKAE
                     MHQSVVEVGTNICKDVSEARKEVTYLRQMVAKLAQKQGLVIGAAGTHPFSRWQDQLIT
                     DHPRYEEIIREMQDAARSNLIFGLHVHVGIDDREIGIRLMNQARYFLPHIFALSTNSP
                     FWEGRNTGFKSFRTKVFDKFPRTGIPDEFSSAAEYDAYIQLLVKTNCMDNAKKVWWDI
                     RLHPFFNTIEFRICDIPMRIEETITLAAIMQGIIAKLYLLMQFNLSFRTYSRALINEN
                     KWRAARYGIDDKLIDFGKEKEVETKLLIEEITEFIDDVVGDLGSRKEVDHVFSMMKRG
                     TGADRQLAVFEKTGDLKKVVDYIISETNIDIPVKKEKKK"
     gene            2381..3220
                     /locus_tag="PKU82_09010"
     CDS             2381..3220
                     /locus_tag="PKU82_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HOZ83052.1"
                     /translation="MTNFIPVRVAVLDLYNGEPNEGMRSIRSILERFSKFTVCSFFDV
                     RQKNEVPDLSFDIYISSGGPGCPLPAGEEWEEPFYALWDKLIEHNADTENKKKYAFLI
                     CHSFQMMAHHLGFGVVSERHSPSFGIFPIHKTDDGNNDRIIGRLPEPFYAVDSREWQV
                     TTPDLDKIVHSGGKLLCLEKMRPHVPYERAIMGLRFSDEILGVQFHPEADAEGMYRYF
                     DRPDKKEKIMELYGSEKYYDMIESLSDPNKIELTHHAIIPCFLADAIEHLYPQLKMQK
                     ATV"
     gene            3336..3905
                     /gene="pyrR"
                     /locus_tag="PKU82_09015"
     CDS             3336..3905
                     /gene="pyrR"
                     /locus_tag="PKU82_09015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOZ83053.1"
                     /translation="MTHLILAGKQLDITIERLCHQLIENHGNFKDTCIIGLQSKGVPL
                     SKRICKRLSEFLPESSILHGALDITFFRDDYRRSEKLLVPSRTEIDFIVEGKKVVLVD
                     DVLFTGRTVRAAMDALMAFGRPAKVELLVLIDRKFSRQFPIEPDYTGQAIDTTITDKV
                     QVEWKESGNKDGVWLVSEIKSKTNGKAKR"
     gene            3886..4815
                     /locus_tag="PKU82_09020"
     CDS             3886..4815
                     /locus_tag="PKU82_09020"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOZ83054.1"
                     /translation="MGKLSVKHLIGIKDLKPEDINLILDTAQTFKEVINRPIKKVPSM
                     RDVTVVNLFFENSTRTRLSFELAEKRLSADILNFTASSSSVSKGETLIDTVNNILAMK
                     VDLVVMRHPKPGAALFLSQKVNSVIVNAGDGTHEHPTQALLDAFSIREKLGTLKGKKV
                     VIVGDILHSRVALSNIYCLQKSGAEVMVCGPATLIPKYIEQLGVKVETNLINALNWCD
                     VANVLRIQLERQDIKYFPSLREYIMQYGITLDILKKLQKKIVIMHPGPINRGVEITTE
                     VADSDQSIILDQVENGVAVRMAVLYLLAGQPVG"
     gene            4897..5253
                     /locus_tag="PKU82_09025"
     CDS             4897..5253
                     /locus_tag="PKU82_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOZ83055.1"
                     /translation="MHYTLDKKEKYAVLKLHEQKLNTLISAELKSELLILNSQGFIHI
                     VLDLSDTQYCDSSGLSAILVGNRICRNANGNFIVTEISDPVKKLINISQLDSVLTIVP
                     KIDEALALINKAASVS"
     gene            5271..6041
                     /locus_tag="PKU82_09030"
     CDS             5271..6041
                     /locus_tag="PKU82_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83056.1"
                     /translation="MIKKLLFSLMFISMSGIAVSQCTPDPTIIDPGVYPDSATGLSNG
                     MVGVAYNQVIQVRTFFDTTATINIGGVPFNANFELDSITILGVSGLPPGLTYACNPGT
                     CQFLASSNGCLLLSGTPVTAGVYPITVDIIARGKITNLGNISYGQPMSVTYYTITIDT
                     NSGVIEMLPKDKLSMSQNLPNPFKNKSVVYFNSPVNTKLDFKIYNILGKNIFSETISA
                     KRGVNTYTINAENLSSGIYMYSINTGTANITRRMVISK"
     gene            6041..6955
                     /locus_tag="PKU82_09035"
     CDS             6041..6955
                     /locus_tag="PKU82_09035"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOZ83057.1"
                     /translation="MNFELTILGSSSATPIHQGHPTAQVLNIRERFFMIDCGEGTQGQ
                     FLKYKFKLNKLSHIFISHLHGDHYLGLVGLLSTMHLQGRTTEIHIYGQPELMDVIELQ
                     LRLSQTRLRYQLIFHPVQHYAPAIIYEDDDMLIKSIVLNHRIPCTGYLFSEKPKPRKL
                     IISKLQQHNIPFSFYGRLKDGFDYEDDEGKIIPNTELTISSTAPRSYAFCSDTCYDES
                     IIDEIKGVNLLYHEATFMNDLAERAKTTFHSTSLQAATIAKKAGVDKLLVGHFSARYR
                     DLKPLLDEARTVFKNTELAIEGHKFSVE"
     gene            complement(6958..>8178)
                     /locus_tag="PKU82_09040"
     CDS             complement(6958..>8178)
                     /locus_tag="PKU82_09040"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOZ83058.1"
                     /translation="LQNPDSLFCKNTTLQQMKNGMQHNIPISASFKAMKYFNLTPAIN
                     FNERWYIETYKQRFDADSNRIMTDTVKGFNAAHEYNGSLSLNTRIYGMKQFKKGKIAA
                     IRHVLSPTLSYMYKPDFGENKYGYYKTVQTDSIGNSRRYSIYENTLYGGPSQGKSNIL
                     SFSLDNNLEMKTRQYTDTAVNIKKIKILESFGLSASYNLSADSFNLSPIGMNARTTLF
                     DMVSLTASGTFDPYLMNDQGIRIAKYSLTENGKIARLTSASGSVGFNLNRQKGNKKSL
                     KGTVEQVKEINDHPDDYVDFSVPYNLSVNYSIAYSRPTNEKGNLSQIVGFNGDISLTE
                     HWKITFNSGYDFQTKKQSYTSLGFYRDLHCWEMRLNWVPFGYQANYYFQINVKSSVLQ
                     DLKLTKKNDRYDNF"
BASE COUNT         2506 a         1451 c         1698 g         2523 t
ORIGIN      
        1 atttaagatt tatcaaaaat aattaaaacc ctattgcata gtagcatcca ataactttta
       61 agctattaat gaaagttgat ttagtttatt aaaggaatct attcaccaac actccaaatc
      121 cttcaacttt caaaaccttg taactttcta accatctaaa cttgttgccc atttgtttct
      181 gtttcttatt tttgtcggat atcatacatt tattatacaa tgaagaaaat aggcattttg
      241 tacggacagg agaatagttt tccaccggca gttgtagagc ggattaacgc taaaaatatt
      301 agtggcattg tggccgaatc ggtgcaggtt gacaaggtgg tgcagggaat gcctatggac
      361 tatgcagtga ttgttgacag aatatcgcat gatgtgcctt tttatcgtgg tatgttgaag
      421 aatgctgcta tttgcggcac agcagtgctg aacaatcctt tctggaacag tgccgatgag
      481 aagtttttta ataactgcct tgccacaaaa attggtgtgc ctgtgccaaa gacggttctg
      541 cttccgagta atgttcatcc gcccgatacg aatgaaaatt ctttcagaaa tctgaagatg
      601 cccttggatt gggataatat ttttaagtac ataggttttc cggcttatat gaaacctttt
      661 gccggtggcg gatggaaaaa tgtgtataag tgcaatgatg ccgcggactt ttataacaag
      721 catcaagaaa caggtcaatt ggtgatgttg ctgcaggagg agattgtgtt tgatcagtat
      781 ttccgttgtt attgtatagg cggcaagcgg ataaagatta tgcgttatga gccacgcaac
      841 ccgcatcacc tgcgttatgt ggttgacggg cctgcacccg agcagcgtat tcttgatgtt
      901 gttgaagaat atacttttaa gctttgcaat gcattgggct atgacatcaa tacggttgag
      961 tttgccattc gtgatggtgt gccttatgcc atagatttct gcaatccggc ccccgatgcc
     1021 gaccgccaca gtgttggcga tgagaatttt aattgggtga tagaagccgt tgccgatatg
     1081 tgtattgaaa aagcactttc gcaccgcgaa ggacaggata acctgacatg gggagctttt
     1141 gttaaacatg ctgcacaagg acagattgtg aacaagcaga atatggatgc actgatggag
     1201 tcttacacgg tgaaataacc agcgtaaaaa agaatatgtc atacattaat aaaaaattag
     1261 ggctgccaca atttacactt ggtgtagaag aggagtttca ggttatagac cctaagacga
     1321 gagagctgcg ctcgcacatg catcagattg tagaaggcgg caaagtgatt ttgaaggagc
     1381 aggtaaaggc cgaaatgcat cagagtgtag tggaagtggg taccaacatt tgcaaagatg
     1441 tttcggaagc ccgcaaagaa gttacctatc tgcgtcagat ggtggctaaa ctggcacaaa
     1501 aacaaggatt ggttattggc gcagcaggta cacatccgtt ttcgcgctgg caagatcagt
     1561 tgattacaga ccatccacgc tacgaagaaa ttatacgcga aatgcaggat gctgcacgaa
     1621 gcaatctgat ttttggtttg catgtgcatg ttggcattga cgacagagaa ataggtataa
     1681 ggctgatgaa tcaggcacgt tattttttgc ctcacatttt tgcactctct accaactctc
     1741 ctttttggga aggaagaaac acaggtttta agtcttttag aactaaggtg tttgataaat
     1801 ttccgagaac gggtattccc gatgagtttt cgagtgctgc cgaatatgat gcctacattc
     1861 aattgcttgt aaaaaccaac tgcatggaca atgctaagaa ggtttggtgg gatataagac
     1921 tgcatccgtt tttcaatact attgaattca ggatttgtga tattccaatg cgcattgaag
     1981 aaactattac gctggcagct attatgcagg gaattattgc caaattgtat ttgctgatgc
     2041 agtttaacct gagtttcaga acctattcca gagcattaat caatgaaaat aaatggcgtg
     2101 cagcccgtta tggtattgat gataagctga ttgattttgg taaagagaag gaggtagaaa
     2161 caaagctact gattgaagaa attacagagt ttattgatga tgttgttggc gatttaggct
     2221 cgcgcaaaga ggtagatcat gtttttagta tgatgaaaag aggcaccggt gccgacaggc
     2281 agttggcagt ttttgagaaa accggagacc ttaaaaaagt ggtagattac ataattagtg
     2341 aaacaaatat tgatattccc gtaaaaaagg aaaaaaagaa atgacgaatt ttattcctgt
     2401 gcgcgttgca gtactcgact tatacaatgg tgaacccaat gaaggcatgc gcagcatccg
     2461 ctctattctt gagcgattca gcaaatttac agtgtgtagc ttttttgatg taagacaaaa
     2521 aaatgaagtg cctgacctga gtttcgatat ttatatttcg tcaggcgggc ccggttgccc
     2581 tttgcctgca ggtgaggaat gggaagagcc tttttatgca ctgtgggata aactcataga
     2641 gcacaatgct gatactgaaa ataagaagaa atatgctttt ctgatatgtc actcatttca
     2701 gatgatggca catcatttag gctttggcgt agtgagcgaa agacattcgc cttcgttcgg
     2761 aatttttcct atacataaaa ccgatgatgg aaataatgac agaattattg gtcgccttcc
     2821 cgaaccgttt tatgctgttg actcaagaga atggcaggtg acaactcccg atcttgataa
     2881 aattgtgcac agtggtggca aacttctttg cttagagaaa atgagaccac atgttccgta
     2941 tgagcgtgca ataatgggat tgcgatttag tgatgaaata ttgggcgtgc agtttcatcc
     3001 ggaagccgat gcagaaggta tgtatcgcta ttttgatcgc cctgataaga aagaaaaaat
     3061 tatggagctt tatggcagcg aaaaatatta tgatatgatt gaatcgctta gcgaccccaa
     3121 taaaattgag ctgacacacc atgccattat accatgtttt ttggcagatg ccatagagca
     3181 tttgtatccg caattaaaaa tgcaaaaggc aacagtttaa taatcggcat agctttattg
     3241 taatttactc cattgcaaaa ttttactgta aatttttgag tggcaaggta atttgatttt
     3301 ttacaattag tttcaaatgc tatttttgcc acacgatgac acatttaatt cttgcgggca
     3361 aacagttaga tattactata gagcgccttt gtcatcagtt gatagaaaac cacggaaatt
     3421 ttaaagatac ctgtatcata ggacttcagt cgaagggagt tccattatct aagcgtattt
     3481 gcaaaagact ttcggagttt ttgccggaaa gcagtattct tcatggtgca ttggatatta
     3541 catttttcag agacgattat cgcagaagtg aaaagctgct tgttccatcg cgcacagaga
     3601 tagattttat tgttgagggc aagaaggttg ttttggttga cgatgtattg tttaccggac
     3661 gaacagtaag agcagcaatg gatgcactca tggcctttgg gcgacctgcc aaggtagagc
     3721 tactggtttt aattgacaga aaattcagca gacagtttcc gattgaacct gattatacag
     3781 ggcaggcaat tgatacaacc attactgata aagtgcaggt agagtggaag gagagcggca
     3841 ataaagatgg cgtgtggtta gtttctgaaa ttaaatcaaa aacgaatggg aaagctaagc
     3901 gttaagcatc ttatcggaat caaagatctg aaacctgaag atatcaacct tattcttgat
     3961 acggcacaaa cttttaaaga agtcatcaat cgcccgataa agaaagtgcc ttccatgcga
     4021 gatgttacag tagtgaatct gttttttgaa aattctacac gcacacgcct ttcattcgag
     4081 ttggcagaga aaagactctc tgctgatata ctaaatttta cagcttcttc atcatcggta
     4141 agcaaaggcg aaacattaat tgatacggta aataatattc ttgccatgaa ggttgatttg
     4201 gtagtgatgc gtcatccaaa acccggagca gctttgtttt tatcgcagaa agtaaattct
     4261 gttattgtta atgcaggcga tggtacacat gaacacccta cacaggcact actcgatgca
     4321 ttttctatac gcgaaaaatt aggaacacta aaaggcaaaa aagtagttat agtaggtgat
     4381 attttacatt caagagtggc attgtcaaat atttattgtc tgcaaaaatc gggtgcagaa
     4441 gtgatggttt gcggcccggc tacattgata ccaaaatata ttgaacagct aggtgtgaaa
     4501 gttgaaacca acttgattaa tgcattgaac tggtgcgatg tagctaatgt attgcgcatt
     4561 cagcttgaaa ggcaggatat aaaatatttt ccctctttaa gagagtatat tatgcaatac
     4621 ggcatcaccc tcgacatact gaaaaagtta cagaaaaaga ttgtcatcat gcatcccgga
     4681 ccaatcaacc gtggggttga aattactact gaagttgcgg attccgatca gtctataatt
     4741 ttagatcagg tcgaaaatgg cgtggccgtg cgcatggcag tactttatct attagccggt
     4801 cagccggtag gttgatttta taagtcgaaa gaccaatttg tttttaatta aaatatcttt
     4861 tttatatttg tttgcaccaa ttttataata atactcatgc attatacctt agacaagaaa
     4921 gaaaaatacg cagtgctaaa gcttcacgaa caaaagctta atacacttat atctgcagaa
     4981 ctgaagtcgg aattgttgat actcaacagt cagggcttta ttcatattgt gcttgatctt
     5041 tccgacacac agtattgtga ctcttcagga ctgagtgcta ttttagtagg caatagaatt
     5101 tgccgcaatg ccaatggtaa tttcattgtt actgaaatca gcgatcctgt aaaaaaacta
     5161 atcaacattt ctcaacttga ttctgtatta accattgtac caaaaattga tgaagcattg
     5221 gctctaatca ataaagccgc ttctgtaagt taaatttaaa attctgaaaa atgataaaaa
     5281 agctactctt ctcattgatg tttatctcta tgtcaggcat tgcagtttcg cagtgtactc
     5341 ctgacccaac aataattgat cccggggttt atcccgacag tgctacaggt ctgagcaatg
     5401 gcatggtggg tgtagcctac aatcaggtta ttcaggtaag aacatttttt gatactactg
     5461 ccacaatcaa tattggtggt gtgccattca atgcaaattt tgaattggat tcaattacca
     5521 ttttaggtgt ttcgggatta cctcccggat taacttatgc atgcaaccca ggaacgtgtc
     5581 agtttttggc aagcagcaac ggatgtttgt tactttcagg tacgcctgtt acagcaggtg
     5641 tgtatccgat aactgttgat atcatagctc gtggaaaaat tactaacctt ggaaatatat
     5701 cttacggcca gcccatgtcg gtaacatatt atactattac tattgacacc aattcaggag
     5761 taatagaaat gctgccaaaa gataaattga gcatgtcgca aaacctgcca aatcctttta
     5821 aaaacaaatc tgttgtatat tttaattcac ctgttaacac aaaacttgat tttaaaattt
     5881 acaatatact tggtaaaaat attttcagcg aaactatttc tgcaaaaaga ggtgtgaata
     5941 cttatactat caatgcagag aacctgtcgt caggaattta tatgtattct attaatacag
     6001 gcacagccaa cattacaaga aggatggtta tttccaagta atgaattttg aattaacgat
     6061 cttaggcagc agttcagcaa cgccaataca tcaaggtcat cccaccgctc aggttcttaa
     6121 tatccgggag cggtttttta tgattgattg tggcgaaggc acgcaagggc agtttctgaa
     6181 atataaattt aaactcaata aactttctca tatttttatt tcacacctgc atggcgacca
     6241 ttatttaggt cttgtaggtt tgctttcaac catgcatctg caagggcgca ccacagaaat
     6301 tcacatctat ggtcagcccg agttaatgga tgttatagaa cttcaattgc gattgtcgca
     6361 aaccagatta agatatcagt taatttttca tcctgtgcag cattatgcgc ctgcaattat
     6421 ttatgaggac gatgatatgc tcatcaaatc aattgttctc aaccatcgta ttccatgtac
     6481 aggatattta ttttctgaaa aaccaaagcc aagaaagtta attatctcaa aactgcaaca
     6541 acataatatt cctttttctt tttatggcag actgaaagat ggttttgact atgaggatga
     6601 cgaaggaaaa attattccaa atacagagtt gacaatttcg tcaacagcac cacgctctta
     6661 tgctttttgt agcgacactt gttatgatga atcaattatt gatgaaataa aaggagtaaa
     6721 cctgctttat catgaagcta catttatgaa tgacttggca gaacgtgcaa aaacaacttt
     6781 tcattcaaca agtctgcaag ctgcaacaat tgcaaaaaaa gccggtgttg acaaactttt
     6841 agtaggtcac ttttctgcac gctaccgcga tttaaaacca ttgctcgatg aagcaagaac
     6901 ggtatttaaa aatacagaac ttgctattga aggacataaa ttcagtgttg agtaatttta
     6961 gaaattatcg tagcggtcgt ttttcttcgt cagcttcaaa tcctgaagta cacttgactt
     7021 aacattgatt tgaaaataat agttggcctg atatccgaaa ggcacccagt ttaaacgcat
     7081 ttcccagcaa tgcaaatcgc gataaaaacc taatgatgta tatgactgtt ttttggtttg
     7141 aaaatcataa ccggagttaa atgttatttt ccaatgttca gtcagtgaaa tgtcgccatt
     7201 gaaaccaact atctgcgaaa gattaccttt ttcattagtt ggtcgtgagt aggcaatgct
     7261 gtaattgacc gataaattat atggtacaga aaagtcaaca taatcatcag gatgatcatt
     7321 aatttctttt acctgttcga cagttccttt taaacttttc ttatttcctt tctgtcggtt
     7381 cagattaaag ccaacacttc cgcttgctga ggtgagtcgt gcaatttttc cattttccgt
     7441 aagagaatat tttgctattc gtattccctg atcgttcatc agataggggt caaatgttcc
     7501 tgaagctgtt aaactaacca tatcaaaaag agttgttcgt gcattcatgc caataggcga
     7561 gaggttaaat gaatctgccg acaaattgta tgatgctgat agtccaaaac tttctaaaat
     7621 ttttatcttt ttaatgttca ctgccgtatc agtatattgt ctggttttca tttccagatt
     7681 attgtctaag ctaaaactaa gaatgtttga cttgccttgc gaaggaccac catacagtgt
     7741 gttttcatag atggaataac gtctgctgtt accaatgctg tctgtttgta cggttttgta
     7801 ataaccatat ttgttctcac caaaatccgg tttgtacata tagcttagtg ttggtgaaag
     7861 cacatgtctt attgcagcaa tttttccttt cttgaattgc ttcatgccat aaattcttgt
     7921 gttcagactt aatgaaccat tatactcatg tgcagcgtta aatcctttaa ccgtatcagt
     7981 cataattctg ttgctgtcag catcaaatcg ttgcttgtat gtttcaatat accaacgttc
     8041 gttaaaatta atggccggtg taagattaaa atatttcatc gctttaaatg atgcactgat
     8101 tggaatgtta tgttgcattc cgtttttcat ctgctgcagt gtggtgtttt tgcagaatag
     8161 actatcggga ttttgtaa
//
LOCUS       DAOOOL010000110         7970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_887_length_7970_cov_58.129122, whole genome shotgun sequence.
ACCESSION   DAOOOL010000110 DAOOOL010000000
VERSION     DAOOOL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_7970_cov_58.129122"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PKU82_09045"
     CDS             complement(<1..1340)
                     /locus_tag="PKU82_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83059.1"
                     /translation="MLSYIFRIPNRMYKQLSIAFFLLLSAAQSISQNLPLGSWREHLS
                     YTQAQFVANTPQRVYCATDQAVYFIDKSDNSVQRYSKINGLSDVGIRLMDYSHTENVV
                     FVGYVNSNIDLISSTGIINISDVERRTATGDKAINDVAFNGKFAYLATGFGVLALDLI
                     KAEIKETYVIGTTGSEVPVYSVTTDGTYIYAAIAGGILRGEISNPALNNFATWQMVYP
                     NTFTGITFNKMCWLNGNLAVNFHSSSTDSVVTVNTTNFATGTTLISGGFALRQLKTEN
                     DRLYAVNNYSVNEWDNNFQPLRQIYGNVNYQNTDMFGVATGSGNQLYIADKNNGLVNY
                     TDDNNITFTVPNSPASPNASCVFHNGTTLVTTHGTNRWSDSYGTDGYSTFDGVQWKTW
                     NVKTITNSNVDLNQMLDLQAVVSEPGNPGHFFLGSRINGVFEFDNNVPIKMYNK"
     gene            1417..2559
                     /locus_tag="PKU82_09050"
     CDS             1417..2559
                     /locus_tag="PKU82_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOZ83060.1"
                     /translation="MALIAVNTRMLLKDKLDGTGWFAFETLKRIVIQHPEHRFIFFFD
                     RKFNSEFIFSNNIMPVVIYPQARHPWLWYTWFEISLHHAFKKYKPDLFFSPESFICSK
                     ATVPQVAVIHDLNFEHFPQYMPTQVARYYSKWSRIAAQNAQRIGTVSEFSRKDIVSTY
                     EINIEKTDVLYNGIHDMYKPLSVAEQEQVRNQYTAGSRYIIYTGSLHQRKNTANMVRA
                     FAQYKKQYSGELKFVLVSDRSKMEADLQLALSETGIESDLIFTGRLSAKALAQLTASA
                     EAMMYVSFFEGFGIPMIEAMRCGVPVITSNVSCLPEIAGNAALCVDPHSVNAITVALE
                     QVLQHQEVAEQLKQKGKIRQQDFSWDKTAELTWQSLQLVLAEKSKG"
     gene            complement(2570..3028)
                     /locus_tag="PKU82_09055"
     CDS             complement(2570..3028)
                     /locus_tag="PKU82_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOZ83061.1"
                     /translation="MEAFRLTRAKFANLLSGKGAAIKGARWNSIGVELIYTASNRSLA
                     MAEVAVHFTLATLPADYMMVTINIPDSVSVQEMKEKELPTDWRNFPHPISTQIIGDRF
                     VAENKFCVLRIPSVVTLGDYNLLINPKHRDFVKIKMKSVEKFPFDKRILR"
     gene            complement(3032..3529)
                     /locus_tag="PKU82_09060"
     CDS             complement(3032..3529)
                     /locus_tag="PKU82_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOZ83062.1"
                     /translation="MSETKLKEKLNKEINTFFHKSRDWDIQFKTTKKITYEDFLSDKI
                     LMIAAIRAGIPYSLFQLIKGFTPFTEYDWADFLDISTKSLQRYHQSSQHYFKPIHSEK
                     IIEMAEVTKVGLEVFEDMDKLKLWLNTPNYALGKIKPIVLLKDSYGKELVMSELIRIN
                     HGILV"
     gene            complement(3654..5786)
                     /locus_tag="PKU82_09065"
     CDS             complement(3654..5786)
                     /locus_tag="PKU82_09065"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOZ83063.1"
                     /translation="MINFLKKYPFVLLVLIAVIGYYQVALFQNTLKWDVTDQYYPWRM
                     FVVQCLRNGILPFWNPYEQFGYPMHADPQSGVWYPVVWLMSLFGPYTLYSVQLEFMLH
                     VIGGGIGMLFLLQRFHVNPFIALINAIAYMFCGLFVGNAQHLTFTIAACWLPFVILAF
                     KNMAEHNHWRFALHFALFGYLLVSGGYPAFAIILFYILLTVTVFLFIRNRQSKHWMKR
                     FLLHSILAVVLLLLCSSGILYSVFQSSDYLARAAGLSFKVASFGPFSPQSLISLMFPL
                     ASARDSEFFGTDISMSSVFVGMLPLAGFILFIIRPKSRSAWLILAATIVMLFASFGSY
                     TPVFGWFYKTFPMLDLFRFPSVYRLFFITGILLLSGIALSAPDAFSRLKLPFATLGGI
                     AAVATIFFWLKNTNEWNFKPWHDAVNNISFTQAIAFQGFIQLLFIVMMLLALLIKDRT
                     KLLMAMITLSAVNVIVAAQLNIPVTVTYNLKTSEVAKEVKEKAVADFPVPEVRPVVLN
                     NDSGGWVSPFWRNLNLFKKQPAWDGYNSFQLKNYVRFVDDESLRQRQISNNWIYFADT
                     VIAYSEEPIIVAPASLHNVCYVPANEKDLLNIQPASGNSISVKTFIPGKIIVEAKTET
                     SSALTLMQQHYHGWKIYDNDKEVKPVVTDYLFMTIPLAQGTHKIEYRYENKPIHNALW
                     LSGVTLIVLIGFSLYAFFSKNKLQALPK"
     gene            5887..6255
                     /locus_tag="PKU82_09070"
     CDS             5887..6255
                     /locus_tag="PKU82_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HOZ83064.1"
                     /translation="MSIKLLTNYLQHHTLIGVKSGIHRKSFTEIWMVTVQGRFFSRSW
                     NKSNDGWYGEILKTGSGQLKLNDTILNVTVKKIKKDDAIQPKIDLAYLSKYTQPENVS
                     YAHGISQPDYHDFTVEFFIE"
     gene            complement(6268..7806)
                     /locus_tag="PKU82_09075"
     CDS             complement(6268..7806)
                     /locus_tag="PKU82_09075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOZ83065.1"
                     /translation="MATTDDLFKNIISHAKEYGFIFPSSEIYDGLSAVYDYGQYGVEL
                     KKNLRDYWWKAMVQTNPDIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDHVAKIEDKIRKEVEKAAKRFENFDEAMFLSTNARVLEYRQQADKINARFKEA
                     LEAGNLTEVRQIIIDSEIVCPVSGSRNWTEVRQFNLMFSTQLGSVTDDAATIYLRPET
                     AQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQYFVKPG
                     TEMEWYNHWKEKRFNWHLSLGLGAECYRFHDHLKLAHYANAACDIEFRFPFGFKELEG
                     IHSRTDFDLSNHEKYSGKKLRYFDTEQNTSYVPYVVETSIGLDRLFLAVLSTAYNEET
                     LADGSQRTVLNIPPFLAPIKCAVLPLLKKDGLPEVAQKIFDSLRLSYQCHYDEKDAIG
                     KRYRRHDAIGTPFCITVDHQTLTDNTVTLRHRDSMEQQRIGINDLKAILDKELALENI
                     LAKL"
BASE COUNT         2548 a         1541 c         1495 g         2386 t
ORIGIN      
        1 tctttgttgt acatctttat aggaacatta ttgtcgaact caaaaacacc attgatgcgc
       61 gagccaagaa aaaagtgtcc gggatttccg ggctcactga ctacagcttg taaatcgagc
      121 atttgattga ggtcaacatt tgagttggtt attgttttta cattccatgt tttccactgc
      181 acaccatcaa atgttgaata accatcagta ccataactgt cgctccagcg gttagtgcca
      241 tgcgtagtta ccaatgttgt accattatga aaaacacagc ttgcattggg cgaggcaggg
      301 ctgtttggaa ctgtaaacgt tatgttgttg tcgtctgtat agttaaccaa accattattt
      361 ttatcagcaa tgtatagttg attgccactg cctgtagcta caccaaacat gtctgtgttc
      421 tgatagttga cattaccgta aatctggcgc aatggttgaa aattattatc ccattcatta
      481 acagaataat tattcactgc ataaagcctg tcattttcgg tttttaactg tcgcagtgca
      541 aagccaccac taattaatgt tgttcctgtt gcaaaatttg ttgtgttaac ggtgactaca
      601 ctatctgtag aagaagaatg aaaattaaca gccagattac cattaagcca gcacatctta
      661 ttaaatgtta ttccggtaaa agtgtttgga taaaccattt gccatgtagc aaaattattg
      721 agtgcgggat tgcttatttc tcctctaagg attccgcctg caatggcagc ataaatatag
      781 gtgccatcag tggtaactga atacacagga acttcacttc ctgtggttcc gataacgtag
      841 gtttctttta tttcagcttt aattaaatcg agtgctaaaa ctccaaagcc tgttgccaga
      901 tatgcaaact ttccgttaaa tgctacatca ttaatagcct tgtcaccggt agctgttctt
      961 ctttcaacat cagaaatatt aataatgcct gtacttgata tgaggtcaat attggaattt
     1021 acatagccta caaacaccac attttctgta tggctgtaat ccatcagcct tatacctaca
     1081 tctgaaagtc cgttaatttt tgaataacgt tgtacgctgt tgtccgactt atcaataaaa
     1141 tatacagcct gatcggtggc gcaataaacg cgttgtggtg tgttagccac aaactgtgcc
     1201 tgcgtatagg aaaggtgctc gcgccatgag cctaatggta gattttgcga aatggattgt
     1261 gctgcagaga gcaacagaaa aaaggcaatg gacaactgtt tgtacatacg gttcgggata
     1321 cggaatatat aactcaacat gagggaattt attttatggc ctgctatgag tttctattta
     1381 aaatactttt gcactacaaa aatataaata atcgcaatgg cgctgatagc ggtaaacacc
     1441 agaatgttgc ttaaagacaa gttagatgga acaggatggt ttgcattcga gaccctgaag
     1501 cgtattgtca ttcagcatcc cgaacatcgt tttatctttt ttttcgaccg taaatttaat
     1561 tctgagttta ttttcagcaa taatattatg ccggtagtca tttacccgca ggcacgtcat
     1621 ccgtggcttt ggtacacctg gtttgaaata agccttcacc acgcttttaa aaaatataaa
     1681 ccggatctat tcttcagtcc cgagagtttt atttgcagta aggctactgt accacaggta
     1741 gcagtaattc acgatttaaa ctttgaacat tttccacaat atatgccaac acaagttgct
     1801 cgttactata gtaagtggtc gcgcatagct gcacaaaatg cacaaagaat aggtactgtg
     1861 tctgagtttt cgcgaaagga tattgtgagc acctatgaaa ttaacattga aaaaaccgat
     1921 gtgttgtata atggtattca tgatatgtac aagccattat cagtagcaga acaagagcaa
     1981 gtacgcaatc aatatactgc aggaagccgc tatataattt acaccggctc attgcatcag
     2041 cgaaaaaaca cagctaacat ggtgcgtgct tttgcacagt ataaaaagca atatagcgga
     2101 gaattaaaat ttgtattagt gtccgatcga agtaaaatgg aagctgattt gcaacttgca
     2161 ctttctgaaa caggaattga gagcgacttg atttttacag gaaggctttc tgcaaaagca
     2221 ttggcacaac taactgcatc ggcagaggca atgatgtatg tttcgttttt tgaaggattt
     2281 ggtattccga tgattgaagc tatgcgctgt ggtgtacctg taatcacttc caatgtaagt
     2341 tgccttcctg aaattgcagg caatgcagct ctctgtgttg acccacacag cgtaaatgcc
     2401 attaccgttg ctttggaaca agtattacaa catcaggaag ttgccgaaca gctaaaacaa
     2461 aaaggcaaaa tcagacaaca ggatttttct tgggataaaa ctgctgaact aacgtggcaa
     2521 agcctgcaac ttgttcttgc tgaaaaaagc aaaggataag agttagtctt tatctgagta
     2581 ttctcttatc aaaagggaat ttttctacag atttcatttt tattttaaca aagtcacgat
     2641 gctttgggtt gatgagtaaa ttatagtctc ccagagtaac cactgaggga attcgtaaaa
     2701 cacaaaactt attttcagca acaaatctat caccaataat ttgagttgat attggatgcg
     2761 ggaagtttcg ccaatcggtt ggcagttctt tttctttcat ttcctgaaca gaaacgctat
     2821 ccggaatatt tattgttacc atcatataat ctgcaggtaa tgttgctaaa gtaaaatgta
     2881 ctgcaacttc tgccatagct aaagagcggt ttgaagctgt atagatcaat tctacaccaa
     2941 tagaattcca ccttgcgcct ttaatagcag ccccctttcc ggataataaa tttgcaaact
     3001 tggctctggt cagtctgaat gcctccatag actaaacaag aataccatgg ttgattctta
     3061 tcaattctga cattactaat tcctttccat aagagtcttt caataataca ataggtttta
     3121 tttttcctaa ggcgtaattt ggagtattta accaaagttt cagtttatcc atatcctcaa
     3181 aaacttcaag tccgacttta gttacttctg ccatttctat tattttttct gagtgtattg
     3241 gtttgaagta atgttgagat gattgatgat atcgctgtaa tgacttggta gatatatcaa
     3301 gaaaatcagc ccaatcatat tctgtaaaag gtgtaaaacc ctttattaat tgaaacaacg
     3361 aataaggtat gccggctctt attgccgcaa tcattaaaat cttatctgaa agaaaatctt
     3421 catacgttat tttcttcgtt gtcttgaact gtatatccca atctctcgac ttatgaaaga
     3481 aggtgtttat ctccttatta agtttctctt ttaattttgt ttctgacata acaagacatt
     3541 tgtcagcaaa tataagacat ttttctgtaa ctgtcaatta attctatttg cggtaatgag
     3601 aaattaacaa acaggtttgt gaaacaataa attttaaata atattcaata acattacttt
     3661 ggcaaagcct gcaacttgtt cttgctgaaa aaagcataaa gtgaaaagcc tatcagtaca
     3721 attagcgtaa ctcccgaaag ccacaaagca ttatgtatcg gtttgttttc ataacggtat
     3781 tctatcttat gagtaccttg tgctaaagga atcgtcataa ataaataatc ggtaacgaca
     3841 ggtttaactt ctttgtcatt atcataaatt ttccatccat gatagtgctg ctgcatcagt
     3901 gttaatgcag aagatgtttc tgtttttgct tcaacaataa tttttcccgg aataaatgtc
     3961 tttacagaaa tgctattgcc tgatgctggt tgaatattca acaagtcttt ttcgtttgca
     4021 ggcacataac aaacattatg cagcgatgca ggtgccacaa tgattggctc ttcagaataa
     4081 gcaataacag tgtcggcaaa ataaatccag ttgttgctta tctgtctttg cctgagcgat
     4141 tcatcatcaa caaagcgtac ataatttttc agctggaaag aattatagcc atcccatgcc
     4201 ggttgttttt taaacaggtt aagatttctc caaaaaggac ttacccaccc accggagtcg
     4261 ttgttgagta caacaggccg cacttcagga acaggaaaat ctgcaacagc tttttctttt
     4321 acttcttttg ctacttcaga agttttaaga ttgtaagtca ccgttacagg aatgtttaat
     4381 tgtgctgcaa caattacatt aactgcagac aatgttatca ttgccatcag aagctttgtt
     4441 ctatctttaa ttaataaagc cagcaacatc atcacaataa aaagcaactg aataaaaccc
     4501 tgaaaagcta ttgcctgagt aaacgaaatg ttgtttacag catcatgcca gggtttaaaa
     4561 ttccattcat tagtattctt taaccagaaa aaaattgtag ctactgcggc aatgccacct
     4621 aaggtggcaa agggtaactt aaggcggctg aatgcatcgg gagcagatag tgcaatgccc
     4681 gaaagcaaca atattccggt aataaaaaac aatctatata cagaggggaa tcggaataaa
     4741 tcaagcatgg gaaaagtttt gtaaaaccag ccaaaaaccg gggtgtagct tccaaatgat
     4801 gcaaagagca tgacgattgt cgctgcaaga ataagccatg cagagcgcga tttgggtctg
     4861 atgataaaga gtataaaccc tgccaacggc aacataccaa caaacacaga ggacatactg
     4921 atatctgtac caaaaaattc actgtcgcga gcagaagcca atggaaacat tagcgatatg
     4981 agcgactgtg gtgaaaaggg accaaacgag gctactttga atgaaagacc tgctgcccgt
     5041 gcaagataat ccgaactttg aaaaacactg tacaaaatac ctgatgaaca cagtaataac
     5101 aacacaacag caagtatgct gtgtaataaa aaacgtttca tccaatgttt gctctgacgg
     5161 ttgcggataa aaagaaatac tgttactgta agcagaatat aaaataaaat aatggcaaat
     5221 gcaggatatc caccagatac caacaagtaa ccaaacaatg caaagtgcaa agcaaaacgc
     5281 caatgattat gttctgccat gtttttaaaa gcaagtatga caaaaggcag ccaacaggca
     5341 gcaatggtaa aggttagatg ctgtgcattg cccacaaaca aaccgcaaaa catataagca
     5401 attgcattaa tgagtgcaat aaaagggttg acatgaaaac gctgtaataa aaacaacata
     5461 ccaattcccc cacctatgac atgcaacata aattccaatt gcacactgta aagtgtatat
     5521 ggaccaaaca acgacatgag ccagacaaca ggataccaca caccactttg cgggtcggca
     5581 tgcatggggt agccaaattg ctcgtatgga ttccagaaag gcaaaatacc atttcgcaga
     5641 cactgcacca caaacatgcg ccacggataa tactggtctg tgacatccca tttcaatgtg
     5701 ttttgaaaaa gtgcaacctg atagtaacca ataacggcta tcagcaccaa taacacaaaa
     5761 ggatatttct ttaaaaaatt tatcacgggc gtaaaaatag aaggaattag tgatgttgaa
     5821 aatataaatt gataatagat gcttttttcc gatttaaaaa tttacgtttg ccatcaaaca
     5881 ccgtaaatgt ctatcaaact tcttacaaat tacctgcaac atcacacatt aataggagtt
     5941 aaaagcggta ttcacagaaa atcatttaca gaaatttgga tggtaactgt tcaaggacgt
     6001 ttcttttctc gaagctggaa caaaagtaat gacggatggt atggcgaaat tttaaaaacc
     6061 ggttcaggtc agttgaaact caacgacacc atcctgaacg ttactgtcaa aaaaattaaa
     6121 aaagatgatg ccattcagcc taaaattgat ttggcttatc tttcaaaata cacacagccc
     6181 gaaaatgttt cttatgcgca tggcatttca caacccgatt atcatgattt tacagtagag
     6241 ttttttatag aatgaaaaaa atccggctta cagtttagcc agaatatttt ctaatgcaag
     6301 ttctttatct aaaatagctt tcaaatcatt aattccaata cgctgttgct ccatactgtc
     6361 gcggtgacgg agtgtaaccg tattatcagt taatgtctga tggtcaactg taatacagaa
     6421 aggtgttcca atggcatcgt gtctgcggta acgtttacca attgcatctt tttcatcgta
     6481 atgacactga tatgaaagtc ttagtgaatc aaatattttc tgagccactt caggcagtcc
     6541 gtcttttttc aatagaggta aaacagcgca tttaatgggt gctaaaaatg gaggaatgtt
     6601 taacactgta cgttgcgaac catcggcaag tgtctcttca ttgtaggctg tgctgagtac
     6661 tgcaagaaaa agtctgtcga ggcctatact tgtttccacc acataaggaa catacgatgt
     6721 gttttgctct gtatcgaagt agcgtaattt tttacctgaa tatttttcgt gattagataa
     6781 gtcaaaatct gtgcgcgagt gaataccttc aagttctttg aatccaaaag gaaaacgaaa
     6841 ctcaatatca caggcggcat tggcatagtg tgccagcttc aggtggtcgt gaaagcggta
     6901 gcattctgca ccaagaccaa gagaaagatg ccagttaaaa cgtttctctt tccagtggtt
     6961 ataccattcc atttcggtgc cgggctttac gaagtactgc atttccatct gctcaaattc
     7021 acgcatacgg aaaataaact gtcgtgccac aatttcattt ctgaaagctt ttcctgtttg
     7081 tgctatacca aaaggaattt tcatcctacc cgttttctgg acgttcaaaa agtttacaaa
     7141 aattccctgt gctgtttcgg ggcgcagata aatggttgca gcatcatctg taacacttcc
     7201 aagctgtgta gaaaacatca gattaaactg ccgcacctct gtccagttgc gcgagccgct
     7261 tacaggacaa acaatttctg agtctataat tatctgtcta acctctgtaa gattacctgc
     7321 ttctaaggct tctttaaaac gtgcatttat cttgtcggcc tgctgacggt attcaagcac
     7381 ccttgcattg gtagaaagaa acatggcctc atcaaaattt tcgaatcgct tggctgcctt
     7441 ttcaacttct ttgcgaattt tatcttcaat ttttgcaaca tgatcttcta tcagcacatc
     7501 ggcacgatag cgttttttag aatctttatt gtcaatcaaa ggatcattaa atgcatctac
     7561 gtgtccggat gctttccatg ttgtaggatg cataaaaatg gctgcatcta ttccaacaat
     7621 atcgggatta gtctgcacca tagctttcca ccagtagtcg cgcagatttt ttttcagctc
     7681 cacaccatat tggccataat cataaacggc actcagcccg tcatagattt cgctcgaagg
     7741 aaaaatgaat ccatactcct tggcatgtga tataatattt ttaaataaat cgtctgttgt
     7801 agccataggc agcaaaggta cgtggaatag attattgtaa aactttatcg gataatttta
     7861 aagagtaatg cggaatacgg aaacgaaaaa tcttaaaggt ctatagcttc aagatatttt
     7921 tctgctaatg gcttgttcag atatcggata acataaggat tggcagaggc
//
LOCUS       DAOOOL010000111         7885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_902_length_7885_cov_55.139080, whole genome shotgun sequence.
ACCESSION   DAOOOL010000111 DAOOOL010000000
VERSION     DAOOOL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_902_length_7885_cov_55.139080"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PKU82_09080"
     CDS             <1..960
                     /locus_tag="PKU82_09080"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83066.1"
                     /translation="PSGNQILCTGGSMLLSASSGIGFTHQWYKNGNAISGATSSTYSA
                     TSGGSYTVFISSPCGSGTSPAAVLTENTVSASVSPAGTSTVCAGIPFTFTANSGAGYS
                     YRWYRNNVLLAGTGQSINATSAGQYKVEVSINGSCAVISNIAVLDVINNPAPVISPAN
                     ATICNGAAQVFTANNFTGVTYQWYRNSVLIPGATAQTYSATLAGQYKVTQTANGCYKS
                     KSAQLTVTSCREEGEGFIAEAQSNISISPNPFTDQLNIYVPSEKDGNAEIQVLDMLGK
                     VVYRSVITLNQTSEINLHVPAGIYMLSVHTDGKYQVQKIVKSR"
     gene            1073..1510
                     /locus_tag="PKU82_09085"
     CDS             1073..1510
                     /locus_tag="PKU82_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOZ83067.1"
                     /translation="MSGVNKVILVGNLGKDPEMRHIDGNISVAKFPLATSEVYKNKDG
                     QRVTQTEWHNVVLWRQLAETAEKFLKKGSLVYIEGKLRTRNWDDKDGNKKYITEVIGD
                     SMTLLGRKPDENGNPSTGTTERTTSEHPASEAGHSEINDDLPF"
     gene            1593..2903
                     /gene="gldE"
                     /locus_tag="PKU82_09090"
     CDS             1593..2903
                     /gene="gldE"
                     /locus_tag="PKU82_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOZ83068.1"
                     /translation="MPVVSSLLLSNFSSYSFTGELLVILVLLLLSALFSGSEVAFFSL
                     RPAQLQYLRDEKPTYINKIILHLLDQPKKLLATILISNTCVNIAIIIFSSLIFSNSQL
                     LGDNPILIFFVQVFSVTFLITMFGEVLPKVYAAHNNMRILSFMAYPIYIMGKFLTPLS
                     VPLVKSTSLFDKLLKRKRTNVSLDELTHAIEITSDIESPPEEKRILKGIVNFGNIDVK
                     QIMRPLPDMIAFGLQTPFKELLQRVIDAGYSRVPVYESSIDNIKGVLHIKDLLAHIKK
                     ENFDWPLLIRKTYFVPENKKINDLLQEFQQRKIHMAIIVDEFGATQGLITLEDVLEEI
                     VGEIKDEFDDEEPVYTKIDEQTYVFEAKTSLNEVCRILELDMELFDEIEGEKDTLAGL
                     LLEINHTIPRIGEVITFKNLRFTIESADRRKIKRVKLQITQKQE"
     gene            2900..3466
                     /gene="gldD"
                     /locus_tag="PKU82_09095"
     CDS             2900..3466
                     /gene="gldD"
                     /locus_tag="PKU82_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOZ83069.1"
                     /translation="MRKLMYILLFGLLTSCSETPAPKPRGFFRIDMPEKKYTLFTDTS
                     CPYRFETPDYSYVLPDTNRFSEPCWKYILYPKFGAQLYLTYKPIRNNLAQMAEDSRGF
                     AYKHTIKANGIDETVINTQNHVYGVVYDIGGNAASNMQFYVTDSFQHFLRGSLYFNTI
                     PNSDSLQPSLEFIRKDVLHLINTLQWSN"
     gene            3842..5218
                     /locus_tag="PKU82_09100"
     CDS             3842..5218
                     /locus_tag="PKU82_09100"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOZ83070.1"
                     /translation="MPKHSWAEPFKIKMVELLKMTTPQQRKKALQQAGYNTFLLKSED
                     VYIDLLTDSGTSAMSDQQWAGMMLGDEAYAGSKNFYRLEDVVKKYYGFKHLIPTHQGR
                     GAENILSQIMIKKGDVVPGNMYFTTTRLHQELAGATFYDVIIDEAHDSSNLHPFKGNI
                     DINKLDKLVKKFGADKIPYVCVATTVNMAGGQPISMANIKELRKYTAKLGIKVMLDMT
                     RIAENAYFIQQREEGYKRKSIATIVKEICSLTDGATFSGKKDALVNIGGFLALNDDKK
                     FEEASNLVVVYEGLHTYGGLAGRDMEAMATGIIESVSDDHIRARIGQVLYLGDKLNEM
                     NIPIVNPVGGHGIFIDAKKFLPHLKQTQFPAQALAAEIYEDSGVRTMERGIVSAGRNK
                     KTGDHYYPELELVRLTIPRRVYTQAHMDVISESIERVYKNREKIKGLEMIYEPTYLRF
                     FQARFKKR"
     gene            5493..6341
                     /locus_tag="PKU82_09105"
     CDS             5493..6341
                     /locus_tag="PKU82_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83071.1"
                     /translation="MYNKIVGIFTAALTLSLLVTSCSSPPEEKKEPTNVAAKIAPATA
                     PLQNNHLLSVNDAVSLIKNYLDTINNSENAEALRKLPVGGSISAEALKRLTEENPDAN
                     GYMFYGFSDAEPATLFKDVNMLAACKFNFNPDSMVSYETSNETNVYLINDTVKHPYQS
                     LTSEAITNYLSQYTKVIPAAASVHHFAAIKGNMNQYTAFFNENFGDGTVQWPFALFSS
                     DEFLTRLYAATGGENNFGGIRYYFGLDKAAKENRLRIVIFAVNKSGSNVVADNRILER
                     HWPPSE"
     gene            6346..7014
                     /locus_tag="PKU82_09110"
     CDS             6346..7014
                     /locus_tag="PKU82_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83072.1"
                     /translation="MDPFLTRMSATCIEVLVALFGLMIFKKAGLLFRLLILLLLYGAA
                     TDIFGWTFYKLNRSYVLIAVDIYTLAEALLMVFIIESTGLFNKLKHIFKLIYLLLFIC
                     YILAFIMMPLIGDDGAYRGTYSFGYLSTVAVLAAWASLKWIEGAGNKLNSPVFVLLTG
                     VFIYTFCSVFIDSFIGTTTGDAVWWIHDAANITAYLIFAYCFYLIKKTTAIEKSVINL
                     PEKV"
     gene            complement(6992..>7885)
                     /locus_tag="PKU82_09115"
     CDS             complement(6992..>7885)
                     /locus_tag="PKU82_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ83073.1"
                     /translation="TPLNMLSKDGVWQSINLQSVIPSGVNIFFGDILCDSYGQVWTTL
                     ISNGLIVYDPSQQKSVRISQETGGLNVNDVRCIVEDKEGQIWLGTSEGIAVIYSPGNA
                     TGSTPAKAQKILITIDGITQYLLDKEIVTAIAVDGANRKWIGTNSGGVFLMSADGTKQ
                     IQNFNTSNSPLFSNTITSIAIDPVTGEVFVGTDKGLISYVGDATQGTESCSSLKVFPN
                     PVKENYDGPIAVRGVTANGNIKITDIAGNVVYETTAKGGIATWDGNNFEGKRAQTGVY
                     LVFATDEKGDNTCVTKLFLVN"
BASE COUNT         2552 a         1475 c         1465 g         2393 t
ORIGIN      
        1 ccttcgggca atcagattct ttgtaccggt ggttctatgt tgttaagtgc atcatcagga
       61 ataggtttta cacatcagtg gtataaaaac ggtaatgcaa tttctggagc aacaagctca
      121 acatatagtg caacttcagg aggaagttat acagtgttta tttcttcgcc ttgcggcagt
      181 ggaacatcac ctgctgctgt gttaactgag aatactgtct ctgcctctgt aagtcctgca
      241 ggtacatcaa cagtttgtgc aggtattcct ttcacattta ctgccaatag cggtgccggt
      301 tattcctatc gctggtatag aaacaatgtg ttgctggcag gaacgggaca aagtattaat
      361 gctacttctg caggacaata taaagttgaa gtttcaatca acggttcatg tgctgtaatc
      421 agtaacattg ctgttttaga tgtcattaac aatcctgctc cggtaatttc gcctgcaaat
      481 gcaactattt gtaacggagc tgcacaggta ttcacagcaa ataattttac aggggtgact
      541 taccaatggt acagaaacag tgtattaatt ccgggggcaa cagcacaaac ctattctgca
      601 acattagcag gacaatacaa ggttacacaa actgcaaatg gatgttacaa atcaaaatct
      661 gctcaactca cagtaacttc ttgtcgcgaa gaaggtgaag gctttattgc tgaagctcaa
      721 agcaacatca gcatatcgcc aaatccattt acagaccaac tcaatattta tgttccatca
      781 gaaaaagatg gcaatgctga aattcaggtt ttggatatgt taggtaaggt tgtatatcgt
      841 tctgtaatta ccttaaatca aacatcagaa attaacctgc atgttccggc aggaatttac
      901 atgctgtctg ttcacactga tggaaaatat caggtgcaga aaattgtgaa atcgagatag
      961 taaagtcgtt cataaaaaaa gaccccgctg ctgcggggtt tttttatgac caaattgtta
     1021 tatttgtatg cacaaacaaa cacattcgtt acgcagttaa acaacagcaa tcatgtcagg
     1081 agtaaataaa gtaattttag taggcaactt aggaaaagat ccggaaatgc gtcatataga
     1141 tggtaacatc agtgtagcta aatttccatt ggcaacatca gaagtatata agaataaaga
     1201 cgggcaaaga gtaacacaaa cagaatggca taatgtggtt ttgtggcgac aacttgcaga
     1261 gactgcagaa aaattcctga agaaaggtag cttggtatat attgaaggaa aactacgcac
     1321 acgcaactgg gatgataaag acggcaataa aaaatacata acagaagtca ttggcgacag
     1381 catgacactt cttggtcgca agcccgatga aaacggaaat ccttcaacag gcactactga
     1441 gagaacaaca tctgaacatc ctgcatctga ggccggtcat tcagaaataa atgatgattt
     1501 accattttaa gcgcgttgca tagtttttaa tacccaacta agcaacatca tcctatattt
     1561 gcaggaaatt atattgtact caccccctca gcatgcctgt cgtcagtagc ttgttactta
     1621 gcaatttttc atcctattca tttaccggtg agctattagt tattcttgtg ttgcttctac
     1681 tttctgcact tttttcaggc agcgaagttg cctttttctc actacgccct gcacaacttc
     1741 agtatctgag agatgaaaaa ccaacataca tcaataaaat aattctgcat ttactcgatc
     1801 agcctaaaaa gttgttggct acaattttaa tttccaatac ctgtgtaaac attgcaataa
     1861 ttattttttc gtctctcatc ttttcaaact cgcagttgtt gggcgataat cctatactca
     1921 ttttttttgt acaggttttt agtgttacgt ttctcatcac catgtttggc gaagtgctgc
     1981 caaaagttta tgcagcgcat aacaacatgc gtattctttc ctttatggct tacccgattt
     2041 atatcatggg aaaattttta acgccactta gtgttccatt ggtaaaatca acttctcttt
     2101 tcgataaact tcttaagcgc aaacgcacca acgtaagctt ggatgaattg acacatgcca
     2161 ttgaaattac cagcgatatt gaatcgccac cggaagaaaa acgtattctc aaaggcattg
     2221 taaattttgg aaatattgat gtcaaacaaa tcatgcgacc gttgccggac atgattgctt
     2281 ttggcttgca aacaccattt aaagagttgt tgcaacgtgt aattgatgcc ggctattcaa
     2341 gagtgccagt atatgaatct tctattgaca acatcaaagg agtattgcac attaaagact
     2401 tattggcaca cataaaaaaa gaaaatttcg actggccact acttatccgt aaaacatatt
     2461 ttgttcctga aaacaaaaag ataaatgatt tattgcagga gtttcaacaa cgcaaaatac
     2521 acatggctat aattgttgat gagtttggtg caacgcaagg gcttattaca ttagaagatg
     2581 tacttgaaga aatagtaggt gaaataaaag acgagtttga cgatgaagag cctgtttaca
     2641 ccaagattga tgagcaaacc tatgtgttcg aggctaaaac atcactgaat gaagtttgcc
     2701 gtattcttga attggatatg gaattgtttg atgaaataga aggtgaaaaa gacacccttg
     2761 caggactgtt gttggaaatc aatcacacca taccacgtat tggcgaagta ataactttca
     2821 agaacctgcg ttttactatt gaatcagccg acagacgaaa aataaaacgt gttaaactgc
     2881 agattacaca aaaacaggaa tgagaaagtt gatgtatatc ttactttttg gtttactgac
     2941 atcgtgcagc gaaacacctg caccaaagcc acgcggattt tttcgtatag atatgcctga
     3001 gaagaaatat actttattta cagacacctc ctgtccatat cgttttgaaa cacctgacta
     3061 tagttatgtt ttgcccgata ccaatcgctt ttcagaaccc tgttggaaat atattctcta
     3121 tccaaaattt ggtgcacagc tttatcttac ttataagcct ataagaaaca accttgccca
     3181 aatggccgaa gattccagag gctttgccta caagcatacc atcaaagcca atggcattga
     3241 cgaaaccgta atcaacacac aaaatcatgt ttacggtgtg gtatatgata ttggtggcaa
     3301 tgcagcaagc aatatgcaat tttatgttac cgacagcttt cagcattttt taagaggatc
     3361 attgtatttt aataccatac caaattcaga ttcgttgcag ccatcattgg agtttatacg
     3421 caaagatgtt ttacacctga taaacacttt acaatggagc aattaaaaaa atatcgctat
     3481 acattaattt tattggcagt agcgttaatg ctgtgtcaat catcttgcag gcttttcaaa
     3541 aaaaaatgtg attgcccaaa gtggagcagt cataccaaaa aatcacagac ggtttaattg
     3601 cgaaatttta tttctgcttt tattctgatt ttcattttca gaacaaagaa ttttagctgc
     3661 atttttaatc aaactgattt taatcatatt catatcacct taaattgaag tttttatagg
     3721 attttaccaa tgaaagtcaa tttttaggtc ttagctaagt tttactattg caaacgaatt
     3781 tttaattgtt tcaaataaat tcaaaagcaa ttcataaaca catcaaaaaa aataaattat
     3841 tatgccaaaa cattcatggg cagaaccctt taaaataaaa atggttgagt tgttgaaaat
     3901 gacaacacca caacaacgaa aaaaagcatt acagcaagca ggttacaaca cgtttctact
     3961 taaatctgaa gatgtatata ttgacttgtt gacagacagt ggaacttctg ccatgagcga
     4021 tcagcaatgg gcaggaatga tgcttggtga cgaagcttat gccggcagta aaaattttta
     4081 tcgcttagaa gatgtcgtaa aaaaatatta cggatttaaa catcttatcc ctacacatca
     4141 gggccgtggt gcagaaaata ttctgtcgca gataatgatt aaaaaaggtg atgtggttcc
     4201 gggcaacatg tattttacta caacacgcct tcaccaggaa cttgcaggtg caacattcta
     4261 tgatgtcatt attgacgagg cacatgacag ctctaacctg catccattta aaggtaatat
     4321 tgacatcaac aaattagata agttagttaa aaaatttggt gccgataaaa ttccttatgt
     4381 ctgtgtagca actacagtta acatggccgg tggtcagcca atttctatgg ctaatattaa
     4441 agagcttaga aaatatactg caaagcttgg cataaaggtt atgttggata tgacacgcat
     4501 tgcagagaat gcctatttca tacaacagcg tgaagaaggt tataaaagaa aaagtattgc
     4561 aaccatcgta aaagaaatct gcagcctgac tgatggtgca acatttagcg gaaagaaaga
     4621 tgcattggtt aacataggag gctttctggc tttgaacgat gataaaaagt ttgaagaagc
     4681 aagtaatctt gttgttgtat atgaaggact gcacacttat ggtggattag ccgggcgtga
     4741 tatggaagca atggcaacag gcattattga atctgttagt gatgatcata tcagggcgcg
     4801 tatcggacag gtgctatatt taggtgataa gctcaatgaa atgaatattc caattgtaaa
     4861 tcctgtgggt ggacatggaa tttttattga tgctaaaaag tttttgccgc atcttaaaca
     4921 gacacagttt cccgcacagg cattagctgc tgaaatttac gaagacagcg gtgtacgtac
     4981 catggaaaga ggaattgtat ctgcaggcag aaataaaaaa actggcgacc actattatcc
     5041 cgaattagag ttggtgcgac tgactattcc acgcagggtt tatacacagg cacacatgga
     5101 tgtgatttca gaatcaatag agcgtgttta taaaaaccga gaaaaaataa aaggattaga
     5161 aatgatttat gaacctacct atctgcgatt ctttcaggca aggttcaaaa aaagataagt
     5221 gttttttgta attattgatt actaaaggag gctaagagcc tccttttttg ttctgttaca
     5281 actgcagaaa acagtctgct ttttgcatct tgtaccgtca taaagcattt tgtacaattg
     5341 ttgttggaat aagttttttc agaagtatat ttgaaaaata ttaatttaaa acgatttttt
     5401 aataaactaa aataataacc aacgaccgat aatttaatta gtcagacaga aaataaaccg
     5461 ctctttattg ctgactatct aaaaccaaat atatgtataa caaaattgtc ggaattttta
     5521 cagcagccct aacactttcc ctgcttgtaa cctcgtgtag tagcccaccc gaagaaaaaa
     5581 aagaaccgac taatgttgct gcaaaaatag caccagcaac agcgccatta caaaataatc
     5641 acttgctaag tgtgaatgat gctgtgtcgt taatcaaaaa ttatttagac acaattaaca
     5701 attccgaaaa tgccgaagct ttaagaaaac tacctgttgg cggatccata agtgccgagg
     5761 ccttaaaaag attgacagaa gaaaaccctg atgccaatgg ctacatgttt tatggttttt
     5821 ctgatgctga acctgcaacg ttatttaaag atgtaaatat gcttgcagct tgtaaattta
     5881 actttaatcc tgatagcatg gtaagttatg aaacaagtaa cgaaaccaat gtttatctga
     5941 taaacgatac tgtaaaacac ccttatcaat cgctgacatc cgaagccatc acaaactatc
     6001 tgtcgcagta caccaaggta attcctgcag ctgcatctgt ccatcatttt gcagccatta
     6061 aaggaaacat gaatcaatac accgctttct ttaatgaaaa ctttggagat ggaactgtgc
     6121 aatggccttt tgccttgttt tcatctgatg aatttttaac ccggctttat gcagcaacag
     6181 gtggtgaaaa taattttggt ggcatacgtt attattttgg attagataaa gcagcaaaag
     6241 agaatagact cagaattgtt atttttgcag ttaacaaatc gggttccaat gtagttgcgg
     6301 ataatagaat tcttgaacgc cattggcccc catcagaatg ataaaatgga tcctttcctt
     6361 actcgaatgt ccgcaacctg cattgaagtg ctggtggcat tgttcgggtt aatgattttt
     6421 aaaaaagccg gactgctgtt ccggcttttg attttattgc ttttgtatgg cgcagcaacc
     6481 gatatttttg gctggacatt ttataaacta aaccgttcct atgtattgat tgcagtggac
     6541 atttatacgc tggcagaagc actactcatg gtttttataa ttgaatcaac aggattattt
     6601 aacaaactaa agcatatttt taagctaatt tacctgttgc tttttatttg ctatatactg
     6661 gcatttatca tgatgccatt aataggtgat gatggtgctt atagaggaac ttactccttt
     6721 ggctatttgt caaccgttgc tgtgttggca gcatgggcat cgctaaaatg gattgaaggt
     6781 gcaggaaata aactcaacag ccctgttttt gttttactta caggtgtttt tatttacact
     6841 ttctgcagtg tgtttattga ttcttttatt ggcacaacaa caggtgatgc tgtctggtgg
     6901 attcatgatg cagccaacat aactgcttat ctgatttttg cttattgctt ttacctgatt
     6961 aagaaaacca cagcaataga aaagtctgta attaatttac cagaaaaagt ttagtaacac
     7021 aggtattgtc gcctttttca tcggtagcaa aaaccaaata aactccggtt tgcgcacgct
     7081 tgccttcaaa gttgttgcca tcccatgttg caattccacc ttttgctgtt gtttcataaa
     7141 caacgtttcc tgctatatca gtaattttaa tattgccatt tgctgttaca ccacgcactg
     7201 caatagggcc atcgtaattc tctttcaccg gattgggaaa tacttttaaa ctagaacacg
     7261 actctgttcc ctgtgtggca tcaccaacat aagaaatcaa tcccttatca gtgccaacaa
     7321 aaacttcccc cgtaacagga tcaatagcaa ttgatgtaat ggtgtttgaa aacaaaggac
     7381 tgtttgatgt attaaagttc tgaatctgct tggtcccatc tgccgacatt aaaaatacac
     7441 ctccactatt ggtgccaatc cattttctgt tggctccatc aacagctatt gctgtaacaa
     7501 tttctttgtc taaaagatac tgcgtaatac cgtcaatcgt tatcagaatt ttttgtgctt
     7561 ttgccggagt gcttcctgtt gcattgccgg gcgaataaat aactgcaata ccttctgatg
     7621 tcccaagcca tatctgacct tctttatctt caacaataca acgcacatca ttaacattta
     7681 atccacctgt ctcctgcgaa attctaactg atttttgttg cgaaggatca taaacaatca
     7741 atccattgct gattaacgtt gtccacacct gaccataaga gtcgcataaa atatctccaa
     7801 aaaatatatt tactcctgat ggaataactg actgaagatt gatagactgc caaacaccat
     7861 ctttactcaa catattcaat ggtgt
//
LOCUS       DAOOOL010000112         7788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_916_length_7788_cov_48.986680, whole genome shotgun sequence.
ACCESSION   DAOOOL010000112 DAOOOL010000000
VERSION     DAOOOL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_7788_cov_48.986680"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..2016)
                     /locus_tag="PKU82_09120"
     CDS             complement(211..2016)
                     /locus_tag="PKU82_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ83074.1"
                     /translation="MKPGFSIKSEFWSYFKYLLGILKDKFVISIVLSIAVSFLDGIGL
                     AMFMPLLQSVSDDGSSVAFSSTSLGKLHIFTDALQGIGLQLNLVSILGVLIVLFSFKG
                     ILRFLQLFFQVKMLQHYMKTVQIDMTNHIQNLSYEGFLTIDAGKIQNSLTTEVNRMRN
                     GIMHYLLSTQSVIMLLCYMLLAYSANWRFALLVSFGAIISSVVFKRIFSRTKKASTSL
                     SKEGNRFNSYILQAVHYFKYLKSTNYFSFYSKKLKSTIQETEHLNKKIGFYQSITSSV
                     REPVLIVVVSLVILLQVKIFGSGLSTIMLSLLLFYRALIYLLSLQASWQSFIQNIGAF
                     ESFNSTLATMKNLEEKNTGTLPFEFNQKIQLKNITLQYGSKVIINDISIDIPKNKTIA
                     LVGESGSGKTTLANLTTSILKPTKGIIYFDDKESTQFDLNSYRSKIGYISQEPVIFND
                     TIFNNITFWQEPTPQVKERFWMIVELTHLGDFIKGLKHKEDTRLGDHGILISGGQKQR
                     ISIARELFKNVELLILDEATSSLDSETEAIIQKNIDNLHGNFTIIIIAHRLSTIKNAD
                     IIYLIENGKVKDSGTFDVLIGRSERFKRMVELQEF"
     gene            complement(2036..4411)
                     /locus_tag="PKU82_09125"
     CDS             complement(2036..4411)
                     /locus_tag="PKU82_09125"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOZ83075.1"
                     /translation="MKQFLYEKVIHYWYIYILSMVVVLIIANFYLWYATPVYTSTTSV
                     MLGMDDKKYANEDLLAQLGSIENTGSIESQMQIIKSRNIITRTIQQLDFRFSYYLEGD
                     IKTSELYKNCPFELVVDSSIISQNFPNLTLKATSISKFELNYVNPKNNSSQTVNGTFD
                     TPISTDLGTLTFVLKAPKLLKFYKNSLDSKNLYKIYSNTSEDLVRSYSQRLNVSQIKG
                     TRMLLISVDDAVPQKGCDFLNKLTDVYLVYGVEQKNEDSRNSLRFIDGQLDVINNDLL
                     KSEGKLEEYKTENGFVDLLNMTGLIQSDKARIENEISILDIKQGVLKYLYKYIREGND
                     VKYLAPAYLEVTEPILQTLIANLSVLQAEREQTLKLTTEDNPLVQKKNVEIENVKAEI
                     LEKIRSMGDVSNITRQEINSQLNKTNSKLRELPKAEKELTNIQRQALITEQLYTYLLQ
                     KRAETAIILASTISDSKVVDNARTMISPVKPVKGITYTIAVLLGLFIPATFVYFKEIL
                     DDRIKNRKILERNSPIPVLGIVSLTSNENNVVVNLRPHSQVTEAFRSIRSNLQYFHGA
                     QTNNIILITSSISSEGKSFCAINLAAILASGGKKVVMIGCDLRKPQQPANFKLSSSTG
                     LSNYLIGTASIDEIIQHSPEMGLDLILSGPKPPNPSEILLNEKMGQLITLLKSKYDYV
                     IIDSPPIGLISDGLELTKYADVTIYLVRQNVTRKLHLDFINKIYLEGKMKNLCIIFNG
                     VKLGGTVYGYGYGYGYAYGYGYGYGYYEEDQRPKNLLQRVVFWFKKKRIKN"
     gene            complement(4493..5278)
                     /locus_tag="PKU82_09130"
     CDS             complement(4493..5278)
                     /locus_tag="PKU82_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOZ83076.1"
                     /translation="MSFISKKQAIPLILILFLSVSCVPTKKFLYYQNGSPGKAQIDST
                     ISLNTYIHKISPNDLLTIYVSSSSPEASSYFNPPINHDNVPDAETYKALSGYLVDNNG
                     DISLPLVGKLNVKGLTTSEARELIKKHLEKYLINPGIFIYLENFKIVMLGEVSKPGVY
                     VVGNEQVTLSEALAMAGDMTVFGKRTDVLIVRESINGNKEYGTLDMTSPEVFKSPYYY
                     LHSNDIVYVNARKNKIASADLFFRIAPVVISFATLVTLLVIRF"
     gene            5414..6190
                     /locus_tag="PKU82_09135"
     CDS             5414..6190
                     /locus_tag="PKU82_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOZ83077.1"
                     /translation="MSFQNLIYKVENNQATITINRPDKLNALNALTVQEISVAVKQAI
                     ADSEVRIIILTGAGEKSFVAGADISEFEGLNEEQSRALAQAGQDAFFVIENSPKPVIA
                     AVNGFALGGGCELAMACHLRVAAENARFGQPEVKLGLIPAYGGTQRLTKLIGKTKALE
                     LMITTDMINASEALQLGLVNYVVPQAELMNKTHELASKIMKQAPLAVAGVIRCVNDFY
                     KQVNGFQSEVEVFGKCFKTEDNKEGVAAFKEKRNPVFKGK"
     gene            6225..7169
                     /gene="paaA"
                     /locus_tag="PKU82_09140"
     CDS             6225..7169
                     /gene="paaA"
                     /locus_tag="PKU82_09140"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HOZ83078.1"
                     /translation="MEVNIEQLEKDFQAKIDAGINIEPKDWMPERYRHQLIRMMSQHA
                     HSEVVGMLPEGNWITRAPSLMRKMVLLAKVQDEAGHGLYIYSATETLGISRDEMTNQL
                     LTGKAKYSSIFNYPTLNWADMGAIGWLVDGAAIVNQTALAKCSFGPYARAMLRICKEE
                     NFHQKQGYQILANMMQGTEEQKQMAQDSVNRFWWPSLMMFGPSDKNSPNSDELMRWKV
                     KLYSNDDLRQRFIDRTVPQAEAIGIKIPDNKLKWNEKTRHYEWGEINWDEFYAVIGGN
                     GPCNRLRMKQRTDAHKNGQWVREAANAFAEKRKKSKAA"
     gene            7210..7497
                     /gene="paaB"
                     /locus_tag="PKU82_09145"
     CDS             7210..7497
                     /gene="paaB"
                     /locus_tag="PKU82_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HOZ83079.1"
                     /translation="MSDIQLKLWEVFTQKKTGMSFEHAGSVRAADKEMALLNARDVYS
                     RRGEATCIWVVPTECISSSSPEDVASFFEPSDDKIYRHPNFYKTPDGIKQV"
     gene            7503..>7788
                     /locus_tag="PKU82_09150"
     CDS             7503..>7788
                     /locus_tag="PKU82_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HOZ83080.1"
                     /translation="MNKNLFEYTLRLGDTSLILSQRLSEWCGHGPMLEEDIALTNIAL
                     DLLGQAKAFLSYAGDIENKERGEDDLAFHRDANQFRNLLLTEQPNVDFGFT"
BASE COUNT         2518 a         1277 c         1385 g         2608 t
ORIGIN      
        1 ggcaagagca gttccaaatg cacctccacc tgctatggta atcttcattg atatttaaaa
       61 taattaatta tttcgagcca aagataaaaa acaaaaggat taattgtagc attttatagg
      121 ctactaatgc tcgcaatttt gatagagcag aaaatagatt tccgtattta aataagctat
      181 ggaattttta ttgatagaaa ctttaaattt ttaaaattcc tgtagttcaa ccattcgttt
      241 aaaacgttcg gaacgaccta ttagaacatc aaacgtgccg gaatctttta cttttccatt
      301 ttcaatgaga taaataatat ctgcattttt aattgttgaa agtctgtgtg caataattat
      361 tatggtaaaa ttaccgtgca aattgtcaat gtttttttga attattgctt cagtttctga
      421 atctaatgag gaggtagctt cgtcaagaat taataattca acatttttaa aaagttcgcg
      481 tgcaattgaa atgcgttgct tctgacctcc tgaaattagt atgccatgat cacctaatct
      541 ggtatcttct ttatgcttta aacccttaat aaaatcgcca aggtgggtga gttctacaat
      601 catccaaaat ctctctttaa cctgaggtgt tggctcttgc cagaaagtta tattgttaaa
      661 gattgtatca ttgaaaataa caggttcctg cgaaatatat ccgattttac ttctatatga
      721 attcagatcg aattgtgtag attccttatc gtcaaaataa atgatacctt ttgtaggttt
      781 taaaattgat gtagtaagat ttgctaaagt tgtttttcct gagccacttt cacctaccaa
      841 agcaattgtt ttattctttg gaatatcaat tgaaatatcg tttataatta ctttacttcc
      901 atactgaagt gttatgttct ttaactgaat tttttgattg aattcaaatg gaagagttcc
      961 ggtatttttc tcttctaaat ttttcattgt agcaagtgtt gaattgaatg attcaaaagc
     1021 tccaatgttc tgaataaaac tttgccatga tgcctgtaaa gacaacaagt atattaatgc
     1081 tctgtagaat aaaagcaagc tgagcatgat ggtacttaat ccactgccga atatttttac
     1141 ttgtaataga attaccaagg atacaacaac aataagtaca ggctctctaa cacttgatgt
     1201 tatgctttga taaaaaccga tttttttatt cagatgctca gtttcttgta tggtactttt
     1261 tagttttttt gaatagaatg aaaaatagtt ggttgatttc agatatttga aatagtgaac
     1321 ggcttgaaga atgtaggagt taaaccgatt cccttcttta gaaagtgatg ttgaagcttt
     1381 ttttgtacgg ctgaagattc ttttgaaaac tacacttgaa attattgcac cgaaagaaac
     1441 caataaggca aaacgccaat ttgcagaata agctaagagc atatagcaaa gcagcataat
     1501 aactgattga gtggaaagca ggtaatgcat tattccatta cgcatccgat tcacttctgt
     1561 ggtcaagctg ttttgtattt tgcctgcatc tattgttaaa aaaccttcat aacttagatt
     1621 ttgaatatga ttagtcatat caatctgaac ggttttcata taatgttgaa gcatcttaac
     1681 ctgaaaaaaa agttgcaaaa acctaagtat gcctttgaat gaaaaaagta ctatcaacac
     1741 tccaagtata gaaacaagat ttaactgaag accaattccc tgtaatgcat cggtgaaaat
     1801 gtgaagtttg cctaatgaag ttgaagagaa agcaacacta ctgccatcat cactaacaga
     1861 ctgtagcagc ggcataaaca ttgccaatcc aataccatct aaaaaactaa ctgcaatact
     1921 taaaacaatg cttataacaa acttatcttt cagaatacct aaaagatatt tgaaatatga
     1981 ccaaaactcg ctttttatac tgaaaccagg tttcactttt tgttatagaa aacagttaat
     2041 ttttaattct ttttttctta aaccagaata cgactcgctg taataaattt ttgggtcttt
     2101 gatcttcttc ataatatcca tacccatatc catatccata agcataacca tacccatatc
     2161 catatccata aactgttcca cccaatttaa caccattaaa aattatacat aggtttttca
     2221 tcttgccttc caaatatatt ttattaataa aatcaagatg cagtttacgt gtaacattct
     2281 gtctcaccag ataaatcgta acatcagcat atttggttaa ttcaagtcca tcgcttatta
     2341 acccgatagg aggagagtca ataattacat aatcatattt gctttttaat aatgtaatca
     2401 actgacccat tttttcattg agcaatattt cagaaggatt aggtggtttt ggtccggaaa
     2461 gtatcagatc caatcccatt tcaggagaat gctggattat ctcatctatt gatgcagtgc
     2521 caatgaggta attgctaagt cctgttgaag atgataattt gaaatttgct ggttgttgag
     2581 gcttgcgtaa atcacaacca atcatcacta cttttttgcc acctgatgct aatattgctg
     2641 caagattaat cgcacaaaaa cttttacctt cagaactgat agaagaagta ataagtatga
     2701 tgttgttggt ttgtgcacca tgaaaatatt gcaggttgct tcgtattgaa cgaaatgctt
     2761 ctgttacttg tgaatgtggt cttaaattta caactacatt gttttcatta gacgttaaac
     2821 ttacaatgcc gagtacagga attggagaat ttctttctaa tattttgcga ttttttattc
     2881 tatcgtcaag tatttcttta aagtaaacga atgttgcagg aataaataat cctagtaata
     2941 cagcaatggt gtatgtaatt cctttcaccg gctttactgg tgaaatcatt gttcgtgcat
     3001 tgtcaactac cttactgtct gaaattgttg atgctaagat gattgcagtc tctgcacgct
     3061 tttgtaatag gtaagtatat aattgttcgg taattaaagc ttgtctttga atattagtca
     3121 attccttctc tgcttttggc agttcgcgta attttgaatt cgttttattt aattgactgt
     3181 ttatttcttg tctggtaatg ttgcttacat cacccatgct tctaattttc tctaaaatct
     3241 cagcttttac attttctatt tcaacatttt ttttctgaac taatgggttg tcttctgttg
     3301 ttaattttaa tgtctgttca cgttcggctt gtaaaacact taggttggcg attagagttt
     3361 gtaatattgg ttctgtaact tcaagataag ctggggcaag gtattttaca tcattccctt
     3421 ctcttatgta tttatataaa tattttagaa caccttgttt aatatctaaa atagaaattt
     3481 cattttcaat acgcgcttta tcagactgaa ttaaaccggt catgttcagt aaatcaacaa
     3541 agccgttttc tgttttgtac tcttctagtt taccttccga tttcaataga tcattattga
     3601 taacatctaa ttgaccatct ataaatctca atgagtttct tgaatcttca ttcttttgtt
     3661 caacaccata aacaagatac acatctgtca atttgttcaa aaaatcgcaa cctttttgtg
     3721 gtactgcatc atcaacagaa atcaacaaca ttcgagttcc cttaatttgg gaaacattta
     3781 aacgctgtga ataggagcgc accaaatctt cagaagtatt tgaataaatt ttatataaat
     3841 tctttgagtc taatgaattt ttataaaatt tcaataactt gggtgcttta agtacaaaag
     3901 ttaaagtccc taaatctgtt gaaataggag tgtcaaatgt tccattaacg gtttgcgaac
     3961 tgttattttt agggttaaca taattaagtt caaacttact aatgctagtt gctttgagag
     4021 taagattggg aaaattttgt gaaattatag atgaatcaac taccaattca aatggacagt
     4081 ttttatataa ttcagatgtt ttaatatcac cttccagata atatgaaaat cggaaatcaa
     4141 gttgttggat agtgcgagta ataatatttc tcgactttat tatttgcatc tggctttcaa
     4201 tactaccagt attttcaatg gaacctaact gtgccagcaa atcttcatta gcatatttct
     4261 tatcatccat gccaagcatg acagatgtag ttgaagtgta aacgggtgta gcataccaaa
     4321 gataaaagtt tgcaattatc aacaccacta ccatacttag tatatatata taccaataat
     4381 gaattacttt ctcatataag aactgtttca agttgaaact ttgatgctcc gggcctgttt
     4441 gttgaaattc gtcttgcatt gattaaaagc gagctttgct taattacttt atttaaaatc
     4501 tgatgactag taaggttact aatgttgcga aactaattac gactggtgca attcgaaaaa
     4561 ataaatcagc agaagcaatt ttattttttc tcgcatttac atagacaata tcgtttgaat
     4621 gaaggtagta ataaggagat ttaaaaactt caggtgatgt catatctaaa gttccgtact
     4681 ctttattgcc gtttattgat tctcttacaa ttaaaacatc agttctttta ccaaacacag
     4741 tcatatctcc tgccattgct aatgcttccg aaagagttac ttgctcatta ccaacaacgt
     4801 aaactccagg cttagatact tcacctagca taacaatttt gaagttttca agatagataa
     4861 atatgcctgg atttatcaga tatttttcca aatgcttttt tattagctct cttgcttcgg
     4921 atgtggtgag tccttttacg tttagtttac ctactaacgg aagactgatg tcaccattat
     4981 tgtcaactaa atatccgctt aaagctttgt atgtttctgc atctggaaca ttatcatggt
     5041 ttataggagg attaaaataa gaacttgcct ccgggcttga acttgacaca tatattgtta
     5101 ataaatcatt tggtgatatt ttatgaatat atgtgttcag agaaatcgta ctgtcaatct
     5161 gcgcttttcc tggagatcca ttttgatagt ataggaattt ctttgtcggt acgcaggaaa
     5221 ctgaaagaaa caaaataaga atcagcggaa tagcttgttt tttcgatata aaactcattg
     5281 agattttttc ttcaaatgta atgggcggca aagataatag aaaaattaag ctttttaatc
     5341 cacgaccgtc aatttagggt attgtttgta gctttcattt ttataatttc gcagctaatc
     5401 catttttcta attatgtctt ttcagaactt aatttataag gttgaaaata accaagctac
     5461 gattacaata aaccgtcctg ataaacttaa tgcactgaat gcattaacgg ttcaggagat
     5521 ttctgttgct gtaaagcaag ctattgcaga tagtgaagtc agaataataa ttcttacagg
     5581 cgcaggtgaa aaatcattcg ttgccggtgc agatatatct gagtttgaag ggctgaacga
     5641 agaacagtca agggcattgg ctcaggccgg acaagacgct ttttttgtaa ttgaaaattc
     5701 tccaaaaccg gttattgcag ccgtaaatgg ctttgcgctt ggaggtggtt gcgaactggc
     5761 aatggcttgc catctgcgtg ttgcagcaga aaatgccaga ttcggacaac ctgaggttaa
     5821 attaggtctg attcctgcct atggcggcac acagcgcctg actaagctta ttggtaaaac
     5881 aaaagcattg gaattgatga ttactaccga tatgataaat gcttctgaag cacttcagtt
     5941 aggcttggta aattatgtag ttccacaagc agaactgatg aataaaacac atgaattggc
     6001 ttcaaaaatt atgaagcagg caccattggc agttgcaggt gttattcgtt gtgtgaatga
     6061 tttttacaaa caggtgaatg gctttcagtc agaagtagaa gtttttggga agtgttttaa
     6121 aacagaggat aacaaagaag gtgttgcagc ttttaaagaa aaaagaaacc ctgtttttaa
     6181 aggaaagtaa cttttggtta ctactattgt tttgattaaa ttttatggag gtgaatatag
     6241 aacaattgga aaaagatttc caggcaaaga ttgatgcagg tatcaacatt gaacctaaag
     6301 attggatgcc tgaacggtac agacatcaac ttataagaat gatgtcgcaa catgcccaca
     6361 gcgaggttgt tggaatgttg cctgaaggca attggataac acgagcgcct tcgttaatgc
     6421 gaaagatggt tttattagcc aaagtacaag acgaagcggg gcatgggctg tacatttaca
     6481 gtgccacaga aactttaggc attagtcgtg atgaaatgac taatcaacta ctgacaggta
     6541 aggcaaaata ttcaagtatt tttaattacc ccacattaaa ctgggcagat atgggagcta
     6601 ttggatggct tgtggacggt gcagcaattg taaaccaaac tgcattggca aaatgctcct
     6661 ttggtcccta tgcaagagca atgctgcgta tttgtaaaga agaaaatttt catcaaaaac
     6721 aaggctatca gattttagcc aatatgatgc agggcactga agaacaaaag caaatggcac
     6781 aggattcagt taaccgtttt tggtggccat cattgatgat gtttgggcca agtgataaaa
     6841 actctcctaa ctctgatgaa ctcatgcgtt ggaaagttaa actctatagt aatgacgatt
     6901 tacgtcagcg ttttattgac agaacagtgc cacaggcaga agcaattgga ataaaaatac
     6961 cagataataa attgaagtgg aatgaaaaaa cgcgtcatta tgaatggggc gaaataaact
     7021 gggatgagtt ttatgcagtt attggtggta atggtccttg caaccgactt agaatgaaac
     7081 aacgtaccga tgctcataaa aatggacagt gggtacgtga agctgctaat gcatttgcag
     7141 aaaaacgcaa aaagagtaaa gcagcataaa caatcaattt caatatttat ctttttaatt
     7201 taataaatca tgtccgacat acaattgaaa ttatgggagg tgttcaccca aaagaaaacc
     7261 ggaatgtctt ttgagcatgc aggaagtgta agagcagcag ataaagaaat ggcattactt
     7321 aatgcccgcg atgtttacag cagacgtggc gaagcaacct gcatttgggt agttcctact
     7381 gagtgtattt catcttcgtc acctgaagat gttgcatctt tttttgagcc ttctgatgat
     7441 aagatttatc gccacccaaa tttttataaa acgcctgatg gcattaagca ggtttaataa
     7501 tcatgaataa aaatttattt gaatatacat tgcgtttagg cgacacaagt ctcattttaa
     7561 gtcagagatt gagtgaatgg tgcggacatg gcccaatgct tgaagaggat attgctctta
     7621 ccaatattgc acttgattta ttagggcagg caaaagcttt tctttcctat gcaggtgata
     7681 tcgaaaataa agaacgcggt gaagatgatt tggcctttca tagagatgca aaccaattca
     7741 gaaatttatt gcttacagaa caacctaatg ttgattttgg atttacaa
//
LOCUS       DAOOOL010000113         7773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_921_length_7773_cov_36.143949, whole genome shotgun sequence.
ACCESSION   DAOOOL010000113 DAOOOL010000000
VERSION     DAOOOL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_921_length_7773_cov_36.143949"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1023)
                     /locus_tag="PKU82_09155"
     CDS             complement(145..1023)
                     /locus_tag="PKU82_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ83081.1"
                     /translation="MKLTIKGLTKTYPNGVQALKNINLEIKNGLFGLLGPNGAGKSSL
                     MRTIATLQNADSGNIFLDDLNVLEDKEEVKKILGYLPQEFGVYPNISAQELLTHLALL
                     KGVHIKKERDEVVKSLLMQTNLYEVRNKNLGGFSGGMKQRFGIAQALIGNPKIIIADE
                     PTAGLDPTERNRFLNLLSEISEQVIVILSTHIVEDVKELCSQMAIINKGEVMFSGHPA
                     EAIKQVQGKIWMCQVDKPTARELMIKYNVISQKFNTGLIELHVWSEEPLNHHFAIAQP
                     VLEDVYFSVLNGNLKP"
     gene            complement(1097..1450)
                     /locus_tag="PKU82_09160"
     CDS             complement(1097..1450)
                     /locus_tag="PKU82_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83082.1"
                     /translation="MKRFKILTIILVAAILPALAQNTQPPVVVPALISQGEVIPYQQL
                     PEVIITPNAAEQKAVELPVVTITANKINEQIYPAVKYGNNYIASVTIAPINVVAHQQK
                     RTFASLFSFLRYFSR"
     gene            1733..2074
                     /locus_tag="PKU82_09165"
     CDS             1733..2074
                     /locus_tag="PKU82_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOZ83083.1"
                     /translation="MHHFLFSYGTLQLDQVQLENYGRKLKGNKDLLKGYRIEDLEIAD
                     SEVIRKSGKHIHPVAVHSGNENDIIEGMIFEITEDELQATDKYEVSDYMRVIEKFESG
                     KEAWIYIGKKK"
     gene            complement(2082..3311)
                     /gene="serA"
                     /locus_tag="PKU82_09170"
     CDS             complement(2082..3311)
                     /gene="serA"
                     /locus_tag="PKU82_09170"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOZ83084.1"
                     /translation="MSLTSFPKEKIKILLLEGVHPAAVAMFKDAGYTQIETTAGALSE
                     KELLQKINNIHILGIRSKTQLTAAAIAQADKLIATGCFCIGVNQVSMGDAIENGIAVF
                     NSPFSNTRSVAELVIANCIMLLRRIPEKSKAALAGQWLKDSAGCFEVRGKTLGIIGYG
                     HIGSQVSVLAEALGMQVIFYDVEPKLSLGNAKACKNINDLLRQSDIVTLHVPGTSATR
                     NMVNESFLRKMKKGSVLINLSRGEVMDEKAIAKAISEKHIAGLAADVFGDEPQHKGDI
                     FQSPLQNLNNVLLTPHIGGSTLEAQENIGTDVAGKIIRYLETGNSMGSVSVPALNLPL
                     QQSAHRILHIHRNVPGVLSEINTIMSKHKANILGQYLQTNNTIGYVAVDIDKKASSGL
                     ISDLKNSKETIRVRGVY"
     gene            complement(3514..5490)
                     /locus_tag="PKU82_09175"
     CDS             complement(3514..5490)
                     /locus_tag="PKU82_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOZ83085.1"
                     /translation="MRALLSIITIIIFQNSVNCQMISNHYLSSKFNRQNSNIASLKDA
                     NNNVIIVGSQSTGMASSNIIIQKYDYLGNLAWVNQTFQTYNSNFRNICIDKECNIYLT
                     ANLNSIGPDRKVYDSRSNKYFQIPVGANSVLAKFNSSGEMINLIAFNGSQLIGVDNSL
                     NIYINDGKIIRVYSQENHELYSFENCEFSKLEIDYNNILIAYKNNYIYLLNTKTGKIL
                     NNFKVDSLIKILPYKSDIYYLEKNGLFKFSKGEKSQILQTTSSNFDLYDDYNFILFNN
                     DSIIKFNLKTKEILNFVYSGSYFLDGIFYIDPSNYIMTGTIGIDGNICAPPLNLKPNR
                     FGINNNNTFFLKGNFINEPYIISEEILKENKTPINDIYIDENNSTFLLKFSTFNCKID
                     PTDLTLQLYLEDKAVCSLATTYSQNYLTFTLPKKLKSGIYTLNFNVRNNSAIKLTGIS
                     VSIINKNFLNEKLDLSNPLLSNQNHLHSNKILFLSANPIETSQLNLEKEKNEIKDVLN
                     SNFTSVFFKFFSFDSVKPSNMVEEMIKLEPTILHFSGHGSESGIILLDEKLHANFIEG
                     KRLADFFSNFKDLQLVVLNACYSQKQAKEIKKYVPYVIGMNAPISDNSAITFSKLFYI
                     ALSNGFTIEESFLKAKAGVNFVKTDSGEMITLLK"
     gene            complement(5648..7432)
                     /locus_tag="PKU82_09180"
     CDS             complement(5648..7432)
                     /locus_tag="PKU82_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOZ83086.1"
                     /translation="MRLFTILTLSFFVFGSNAQVPSYIVDTTCFTALTLEGGQTEIEF
                     ADINNDGHLDLLTVGDHGSPLINSSQHGISVFFGNGTGTGWTLYQNGDFGYGGIAVGD
                     VNNDGFLDVGYGIHHNYSATDFGDQLLEVALGDGTGMNWTPWDDSLATAGETWGMFGT
                     EFGDFNNDGLLDIVSGSFGCCAGTHVYLNTGTGVWNHSFGYINGNTEHYVECGDINHD
                     GNLDFVSCHQNGAAYFGDGTGNFTNSHFNLPSIGPLGFYDISLKDIDGDGDDDFGFIH
                     NNSMPYVYKWDNTTQQWVNSSAGLTVSGLASCIKLSDLNNDGFADAVVATTGSVVIFL
                     GNGGTVWNNVLTIPLTNMTFCQDIAIADADQNGLPEIAVWATFNNGLFSTINKLVYLR
                     EVTPVTTLNINQIFPNGFECLPANAVRNITWNSSVVPGHQTSVKIEFSSTGINGTWTT
                     VATAAPNNGNYQWTVPSGIISYNSYIRTILTDSITLVTDTAYNLHPFQVGVCDPALSI
                     SALDNNFDFNIAPNPFNDAFSISSKNKLVQIEIFNTVGSKVFSEDLKLLPKQSYVVKP
                     NINSNGIYFVKLTTVAGEIKTMKIVREE"
BASE COUNT         2498 a         1326 c         1297 g         2652 t
ORIGIN      
        1 aatgttgcca acagcaccac tgatagaaat tgcatctgta gcagtgaata aaaagctgat
       61 cagaaataaa atagctaaat agatgtaagt tgcaggcctt ctgaaacggt acgacaactc
      121 aaaactgagt acactccaca tagcttaagg ttttaaatta ccgttaagaa cagaaaaata
      181 aacatcttcc aaaacgggct gcgcaatggc gaagtgatga ttaagcggtt cttcgctcca
      241 gacatgcagt tcaatgaggc cggtattgaa tttttgtgaa atgacgttat attttatcat
      301 cagctcacga gctgtaggct tgtcaacctg gcacatccag attttcccct gaacttgttt
      361 tattgcttct gcagggtgac cactgaacat tacttcacct ttattgataa tagccatttg
      421 agagcataac tctttaacgt cttcaacaat gtgtgtagaa aggattacaa ttacctgctc
      481 gctgatttcg ctaagcaaat tcagaaaacg gtttctttct gttgggtcaa gacctgccgt
      541 tggctcatca gcaataataa tttttggatt gccgattaat gcctgagcaa taccaaaacg
      601 ctgtttcatt ccaccgctaa aaccacctaa atttttgttg cgcacctcat aaaggtttgt
      661 ttgcattaac aaacttttca caacttcatc gcgctctttt ttaatatgaa cacctttaag
      721 taaggcaaga tgcgtgagca gctcttgtgc tgatatgtta ggataaacac caaattcctg
      781 tggcagatag cccaatattt tttttacttc ttctttatct tcaagtacat ttaggtcatc
      841 taaaaatata tttccgctat cggcattttg cagcgtagct attgttcgca tcagggatga
      901 tttacctgca ccgttgggac ctaacaaacc aaaaaggccg ttctttattt caaggtttat
      961 attttttaat gcctgcactc cgttgggata tgtttttgtc aggcctttaa tggtaagctt
     1021 cattggcaga aatttgattt ttatgtttgt aaaaaaatga atgctataaa agcagcaagc
     1081 aaaataagag gttttgttaa cggctaaagt agcgaagaaa tgagaacagt gatgcaaagg
     1141 ttcttttctg ctgatgtgcc accacattaa taggtgctat tgttactgag gcaatgtagt
     1201 tgttgccata ctttactgca ggataaattt gttcgttaat tttatttgca gtaatcgtaa
     1261 caacgggtaa ttcaacagct ttttgttcag cagcattcgg ggtaatgatg acttccggaa
     1321 gttgttgata gggtatcacc tcgccctgac tgatcaatgc aggaactact accggtggtt
     1381 gtgtgttttg tgctaatgca ggcaatattg ctgcaactaa aatgatggtt aaaattttga
     1441 agcgtttcat ctgtatatgt ttttattgat tattgcgatg caaagatata tttataaaac
     1501 agtactatgc aacacatagt agtataaaaa attgtaatat attgaaaaac agtcataaaa
     1561 atttaaacta tactattaaa ttatcggttt tttacttttc tgagaactaa taacaagacg
     1621 tttcagatgc ttaaaatgtt acaaaggcag gttaaacagg gtacataaag taataaaaag
     1681 cacctgattc tttattggct taatactaat attgcctcaa caaatttaat ccatgcacca
     1741 ctttttattt tcttatggca cattgcaact tgatcaagtt caactggaaa attatggtcg
     1801 aaaactaaaa ggcaacaaag atttattaaa aggctatagg attgaagatt tagaaattgc
     1861 agacagtgaa gtaattcgta aaagtggaaa gcatattcat cctgtggctg tccattccgg
     1921 aaatgaaaac gatattattg aaggaatgat ttttgaaatt acagaagatg aattacaggc
     1981 taccgataaa tatgaagtgt ctgactatat gcgtgtgatc gagaaatttg aatccggaaa
     2041 agaagcatgg atttatatcg gaaagaaaaa ataataatgc attaataaac cccacgaacc
     2101 ctgatagtct ccttactgtt ttttaaatct gatatcaaac ccgaagaagc ttttttatct
     2161 atgtcaacag ctacataacc tattgtatta tttgtttgca ggtattgtcc gagaatattt
     2221 gctttatgtt tactcattat ggtatttatt tcggaaagaa caccgggcac gttgcggtga
     2281 atatgtaaaa tgcgatgtgc actttgctgc aatggtaaat tcaatgctgg tacagaaaca
     2341 ctgcccatgc tgttgcctgt ttcaagatag cggataattt ttccggctac atcagtgcca
     2401 atgttttcct gtgcttcaag tgttgaccct ccaatgtgtg gagtcaacaa cacattattc
     2461 aagttttgta aaggcgattg aaaaatatcg cctttatgtt gtggctcatc accaaataca
     2521 tcggctgcaa gccctgcaat atgtttttct gaaatggctt ttgcaatagc tttttcatcc
     2581 atcacctcac cacgactcag gttgataagc acactgcctt ttttcatttt tcgtaagaaa
     2641 gattcattca ccatattgcg cgttgccgaa gttcccggca catgcagtgt aacaatgtct
     2701 gattgcctga gtaaatcatt tatattttta catgctttgg catttcccaa actcagtttt
     2761 ggctctacat cataaaaaat tacttgcatg cctaatgctt ctgctaatac agaaacctga
     2821 ctgcctatgt ggccatatcc tattatccct aatgttttac cgcgaacttc aaaacatcct
     2881 gcactgtctt ttaaccattg tccggcaagg gcggccttac ttttttccgg aattctgcgc
     2941 aacaacataa tgcaattggc aatcactaac tctgctacag agcgtgtatt gctgaaaggc
     3001 gaattaaaaa cagctattcc attttctatg gcatcaccca tacttacctg attgacacca
     3061 atacaaaaac agcctgttgc aatgagttta tctgcttgtg caatagcagc agcagtaagc
     3121 tgtgtttttg aacgtatgcc aagaatatgt atattgttta ttttttgtaa cagttctttt
     3181 tccgataatg caccggcagt ggtttctatc tgtgtgtatc cagcatcttt aaacattgcc
     3241 acagcagcag gatgaacacc ttcaagcaat aggattttaa ttttttcttt cggaaaactt
     3301 gtcaacgaca taaatcaatt ttgatgcaat gatagtttgt tttatctgac cggaatagtg
     3361 taaataagac aagttattta cattcagttt aggaaagata aaaccaatta atttatagcg
     3421 aaaatattaa aacgattttt aaaaaagtat attttgtcca ttcgaagtct cccgtatttc
     3481 aggggacttt gaatggatgc aagaaattag tgattattta agcaatgtaa tcatttctcc
     3541 tgaatccgtt tttacaaagt ttacgccagc ttttgctttt aaaaacgatt cttctattgt
     3601 aaaaccattt gaaagcgcaa tataaaatag ctttgaaaaa gtgatggctg aattgtctga
     3661 aattggggca ttcataccta ttacataggg tacatatttt tttatttctt tcgcttgttt
     3721 ttgagaatag catgcattta acactactaa ttgtaaatct ttaaaattag aaaaaaaatc
     3781 tgctaatctt ttgccttcaa taaaattggc atgcaatttc tcatctaaaa gaataatacc
     3841 agattcactt ccatgtccag aaaaatgcaa tattgttggt tctaatttta tcatttcctc
     3901 taccatatta cttggtttta ctgaatcaaa agaaaaaaat ttgaaaaaaa cgctagtaaa
     3961 atttgagttt aggacatctt tgatttcatt tttctctttt tctaaattta gttgagaggt
     4021 ttcaattgga tttgcagata aaaacagtat cttatttgaa tgtaagtgat tttgattaga
     4081 tagtagtggg ttggataagt ctaacttttc attaagaaaa tttttattaa ttatggaaac
     4141 agatatacca gtaagtttta tggcagaatt atttcttaca ttaaaattca aagtataaat
     4201 tccacttttg agttttttag gtaacgtaaa agttaaataa ttttgactgt aagttgttgc
     4261 gagactacat acagctttat cttctaagta aagttgaagt gtaagatcag ttggatcgat
     4321 tttacaatta aatgtagaaa attttaataa aaaagtacta ttgttttcat caatataaat
     4381 atcatttatt ggcgttttgt tttcttttaa tatttcctca ctaattatat aaggttcatt
     4441 gataaaattt cctttaagaa aaaacgtgtt attattatta attccaaagc gatttggttt
     4501 taaatttaat ggtggagcac aaatgtttcc gtcaattcca attgtgccgg tcatgatata
     4561 gttgcttgga tctatgtaaa aaataccatc tagaaaatat gaacctgaat aaacaaagtt
     4621 tagtatttct ttagttttca gattaaattt aataattgaa tcattattga aaagaataaa
     4681 attataatca tcatatagat cgaaattact agaggttgtt tgtagaattt gacttttttc
     4741 tcctttagag aatttgaaca acccgttttt ctccaagtaa tagatatctg atttgtatgg
     4801 tagtatttta attaaagaat ctactttaaa attattcaaa atcttaccag ttttcgtgtt
     4861 tagtaagtaa atgtaattat ttttataagc aattaaaatg ttgttgtaat ctatttctaa
     4921 tttggaaaac tcacaatttt cgaatgagta tagctcatga ttttcttgag aataaactct
     4981 aatgattttt ccatcattaa tgtaaatatt taatgaatta tctacaccga ttaattggct
     5041 tccattaaat gcaattaaat taatcatctc acctgatgag ttgaactttg ctaatacact
     5101 attcgctccc actggaattt gaaaatattt atttgaacgt gaatcataaa cttttctatc
     5161 gggaccaatg ctatttaggt tagcggtcaa ataaatatta cattctttat caatacatat
     5221 atttctaaag tttgaattat atgtttggaa tgtttgatta acccatgcta aattaccaag
     5281 ataatcatat ttttggataa taatatttga acttgccatt cctgttgatt gtgagccaac
     5341 tataataaca ttgttgtttg catctttaag tgaggcaata ttggagtttt gtctattaaa
     5401 tttagaagat aaataatgat tagaaatcat ttgacaattt actgaatttt gaaatataat
     5461 aattgttata atagatagaa gcgcgcgcat ttttttaatt atagatacat tatagcaaga
     5521 attccataaa attttaaaca atgaaagttt tagctattag aataacttta ttatagtttt
     5581 actaaatttt tctattgatt aattattaac attgcggcct gtgtcaatgt aagcttcatt
     5641 aaatttttca ttcctcccta acaattttca ttgttttaat ttcaccggca acagtcgtta
     5701 gcttaacaaa gtaaatgccg tttgaattta tatttggttt taccacataa gattgtttag
     5761 gcagaagttt taaatcttca gaaaaaacct tactgcctac ggtattaaaa atttcaattt
     5821 gcactaactt gtttttcgag ctaatactaa atgcatcgtt aaaaggattg ggtgcaatat
     5881 taaaatcgaa gttgttatct aaagcagaga tagacaatgc agggtcacaa acacccacct
     5941 gaaagggatg caggttgtag gcagtgtctg ttaccagggt tatactatct gttaagattg
     6001 ttcttatgta tgaattataa cttattattc ccgaaggcac agtccattga tagttgccat
     6061 tgtttggtgc tgcagttgca acagttgtcc aggtgccatt aataccggtt gaagaaaatt
     6121 caatctttac agatgtttga tgtcccggaa cgacactact gttccatgta atatttctta
     6181 ctgcattggc aggcaaacat tcaaatccat ttgggaaaat ctgattgatg tttaatgttg
     6241 ttaccggtgt aacttctctg agataaacca atttattaat agtagaaaac agtccattgt
     6301 tgaatgttgc ccacactgca atttcaggca aaccgttttg atctgcatct gcaatggcta
     6361 tgtcctggca aaaagtcata ttggttaaag gtattgttaa aacattattc catactgttc
     6421 ctccgttacc taaaaagata acaacactac ctgttgtagc aactactgca tcggcaaagc
     6481 catcattatt taaatctgac agctttatac atgatgccaa tccagatact gttaaccctg
     6541 ctgaagaatt tacccattgt tgtgtagtgt tatcccattt ataaacataa ggcatactgt
     6601 tgttatgaat aaagccaaaa tcatcatcac catcgccatc aatatcttta agagaaatgt
     6661 cataaaaacc taaaggacca atggaaggca ggttaaaatg actgtttgta aaatttcctg
     6721 tgccatcacc aaaataggca gcaccattct gatggcatga aacaaaatca agattaccat
     6781 catggtttat gtcgccacac tcaacataat gttctgtgtt accatttata tatccgaatg
     6841 aatggttcca cacaccggtg ccagtgttca gatatacatg tgtgcctgcg cagcaaccaa
     6901 acgagcctga aacaatatcc agcaagccat cattattaaa atcgccaaat tctgttccaa
     6961 acatacccca tgtttcaccg gctgtagcaa gcgagtcgtc ccatggtgtc cagttcattc
     7021 ctgtgccatc acctaatgca acttccaaaa gctgatcacc aaaatctgta gcagaataat
     7081 tgtgatgaat gccatagcca acatctaaaa agccatcgtt attgacatcg cccacggcaa
     7141 tgccaccata accaaaatcg ccattctgat aaagtgtcca accggtgccg gttccgttac
     7201 caaaaaaaac actgatgcca tgttgacttg agttgatgag cgggcttcca tgatcgccaa
     7261 cagtcaataa atctaaatgt ccgtcattgt tgatgtcggc aaattcaatt tctgtttgtc
     7321 caccttctaa agttagggca gtaaaacaag tagtgtcaac aatataggaa ggtacttggg
     7381 cattgctgcc gaatacaaaa aaacttaatg ttaaaattgt aaaaagtcgc atattttttt
     7441 ttgataaatt tataaaataa atactgtaat gttttgtttt ctgcttgtag gtattgagtt
     7501 caattttttg tagatatgaa tctaaataaa tcggttaatc ataagggtat tgaatggatt
     7561 ggtagcatta ggttttaata attgaaaagc atattatgct tttccctcag ttgcaactaa
     7621 aaattaccca tttacctctt ctcatttttt tcttcaccac attataaacc gctctttaat
     7681 tatcagcagt aatctcaaca aaataaatgc tcggttctgc atgaatgttc gggtatagtt
     7741 ttacggattt aattctcagt cacaattaat ttc
//
LOCUS       DAOOOL010000114         7668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_937_length_7668_cov_54.377118, whole genome shotgun sequence.
ACCESSION   DAOOOL010000114 DAOOOL010000000
VERSION     DAOOOL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_7668_cov_54.377118"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5241
                     /gene="sprA"
                     /locus_tag="PKU82_09185"
     CDS             <1..5241
                     /gene="sprA"
                     /locus_tag="PKU82_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOZ83087.1"
                     /translation="VDYNLGRVKIINTSVLNSATPIKVSLESNSLFSVQSKTLMGARF
                     DYKFSKDFAVGGTFMHLNERPITRKVNIGDEPISNSIFGLDGTYRTRSRFITKLVDKI
                     PLINTKEESSVSLNGEFAYLLPGHSKAIGKKGNAYIDAFEGTQSTIPLNIAGQWFLAS
                     VPRFQPALFPEFDYVSATNDTLGYGYNRAKMAWYNIDPTAFYRSNSTVSLSAAERSNH
                     NVRQISEKELFPKRQYSNGIVPYMNTFDISFYPDERGPYNYETSARAGLSAGIDANGK
                     LVDPTSRWGGFMRKVETSNFEESNIQYIQFWLMDPFNGDAPDSSMSNTGTLYFNLGDI
                     SEDVLRDGYESYENGLPTTATPAAYRQNAWGRTPVAQNVLYAFDRDESTRKQQDVGLD
                     GLGNDDEKLHFLTNFLDYIDAIVTNQTAKDAFHEDPSSDDFRYFRGDAYTNADGILQR
                     YKNFNGTENNSPVAGSGTFVPSSTQTPDAEDANRINGMEVDENYFQYKVDIDPSKLVV
                     GQNYVTDVLENVAALNLPDASGKTITWYLIKIPVNDPSRERIGQISDLKSVKFIRVFA
                     KGFNGPKVLRLAKFEFLRGEWRKYDYKLTGILSQPAVFDVGAVSLEENSNREPINYVL
                     PPDFEREIDVTSTNQRQLNEQSLSFKVCELADGDMKVCYKNTTLDMRNYKHVKMVIHA
                     ESFLPPANPTNDGELYAVVRLGSDFTDNYYDYRIPLKMTPFGSTDPTVIWPKENDFDI
                     DLDKLTDAKLERNNAGVAFTDEYSKLDGNNVIFIKGNPTLSEVKVMMLGLWNPSGENP
                     AFPPRPPDDGLAKCAEVWFNEFRMSDFNDKGGWATIGRAQVKLADLGNVSVSGGYSTH
                     GFGSLESKVGERSKEDVTNYDVATSLELGKFFPSSTGLTVPMYFGYGEVFSNPQYDPL
                     DPDIEFSRSISNIDDSKRKQNRKRFSQDYTRRKSLNFTNVKKNKTGKNASKSRIYDIE
                     NFNFTYGYTEVYRRNINTEYDIQKDYLGAIGYNFNTNPKPITPFSKMKAKSKYLRPIK
                     DFNFNYMPSTFNFRWDVNRHYGELKVRDVNAVLNQGVSDNSIPVLYDKRFTMNRQYGT
                     SWDITRALKFEFNANNQSKVDEPDGALNSEEKRDSVRKNFFNLGRNTDYMHNASLSYN
                     LPLNKLPITDWISINTRYSTTYHWTASPLIVNTDNGRYYTNPALGNTIQNSNSIQVNN
                     SFNMTSLYNKWKLYKKLTAPPKPKTSAKKEPKVDAKAPDAAKDSLKTKSVKPPEKKKE
                     QEINPAIKALAKVIFSLKNVSFNWNETNGLLLPGFNRQSEWLGQNWDGTSAPGFAFTF
                     GSQNQDIRERAAKNGWLTTDSTFNSQFTSTYTQNITGRASLEPFTGFNIDVNFNRNYS
                     LSTTENYRAYNSPRANGRYYETLARVETGNFSISNLMWGTSFTKDRKDYSSTIFERFA
                     ENRRILSERLGNENPNSSGVGADGYRDGYSGTSQEVVILSFLSAYSKKSASRMSLDLF
                     PSVPLPNWTVRYDGLTKIAFVKNLFQSVNLNHGYRSTYNVNSFTTDVSYVPGGNARDI
                     NMDFIPKRQIGQVSLSEQFSPLIGIDITWKNRNDKSSGSKSATERGGRSSGGNFTTRF
                     EYKRDRNISLTLAGIQVTEVKGREFTFGAGYRIPNFKFPFGLFKSVNSRRSNDLNTTL
                     DFSVRKNTTILRKLVEGTNQPTAGLTIISIKLASDYTVSERLNLRLFFEKTINTPVVS
                     TSYPNANTAFGITIRFTLSQ"
     gene            complement(5275..6399)
                     /gene="carA"
                     /locus_tag="PKU82_09190"
     CDS             complement(5275..6399)
                     /gene="carA"
                     /locus_tag="PKU82_09190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOZ83088.1"
                     /translation="MKYAHKSPAVLLLQDGTVFYGKSAGKIGTATGEICFNTGMTGYQ
                     EIFTDPSYKSQIMVMTSVHIGNYGVCQAESESSSAKISALVCRNFSDHFSRKKADQSL
                     QDYFIENNMVVVSDVDTRAIVQHIRSKGAMNAIVSSEETDLAKLKTLLAKVPSMEGLE
                     LSSTVSTQKAYTYGDAAAALRVAVIDLGVKENTLRCLADRGCYIKVFPMNTSFEEMEK
                     FNPQGYLVSNGPGDPAVMPAMVDTVKKILNSDKALMGICLGHQMLAEACGIKTFKMFN
                     GHRGINHPVKNLVTGRAEVTSQNHGFGVIPEETINKAEIEITHINLNDQSIEGISVKN
                     KKAFSVQYHPEAAPGPNDARYLFDNFVNAVREVKEQAVTV"
     gene            complement(6472..7161)
                     /gene="radC"
                     /locus_tag="PKU82_09195"
     CDS             complement(6472..7161)
                     /gene="radC"
                     /locus_tag="PKU82_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOZ83089.1"
                     /translation="MEEPKNIKSWSESDRPREKLLEKGKEALTDSELLAILIRSGTRQ
                     ESAVDLAKRILKNTHNELSELSKLSVKDMMKYKGMGKVKSITIVAALELGRRRAQAQA
                     LERQKITSSKDAVAYFKALLADLPHEEFRILLLDRANQIIKSENISKGGVSGTVVDAR
                     IIFKSAIENLASGIILCHNHPSGNPKPSQADVNLTSKLSQAGKLLEINILDHVIIAGD
                     DYYSFADEGQL"
     gene            complement(7235..7489)
                     /gene="rpsT"
                     /locus_tag="PKU82_09200"
     CDS             complement(7235..7489)
                     /gene="rpsT"
                     /locus_tag="PKU82_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOZ83090.1"
                     /translation="MANHKSAVKRIRANGAKKDRNRYQAKTSRNALKELRTTTDKKEA
                     EKLLPEVSGMLDKMAKKNLIHKNKAANLKSSITKYVSALK"
     gene            complement(7540..7611)
                     /locus_tag="PKU82_09205"
     tRNA            complement(7540..7611)
                     /locus_tag="PKU82_09205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7576..7578),aa:Glu,seq:ttc)
BASE COUNT         2346 a         1472 c         1551 g         2299 t
ORIGIN      
        1 gttgactata atctaggtcg tgtaaaaatc atcaatactt cggtgctgaa ttctgcaaca
       61 ccaataaaag tttctttaga gagtaattcc ttgttttctg ttcagtcaaa aacattgatg
      121 ggtgcacggt ttgattacaa gttcagtaaa gattttgctg ttggtggtac ctttatgcac
      181 ctcaacgaaa gacctattac acgaaaagtt aacattggtg atgaacctat ctcgaattca
      241 atattcggtt tggatggaac ttatcgtaca cgctcacgat tcatcactaa gttggttgat
      301 aaaattccat tgattaatac taaggaagag tcttcagttt cattgaacgg agaatttgct
      361 tatttacttc ccggccatag taaagcaatt ggtaaaaaag gtaatgctta tattgatgcc
      421 tttgaaggaa cacagagtac cattccgttg aatatagcag gtcagtggtt cttagcttct
      481 gtgccacgtt ttcagcctgc actgtttcct gaatttgact atgtgagtgc cacaaacgac
      541 acattaggtt atggctacaa tcgtgctaaa atggcttggt acaatattga cccgactgct
      601 ttttacagaa gcaacagtac ggtgtcatta tctgctgcag aaagaagtaa tcataatgta
      661 agacaaatta gtgaaaagga attattcccc aagcgacaat acagcaatgg tattgttcct
      721 tacatgaata cattcgatat ttctttttat cctgatgagc gtggacctta caattatgaa
      781 acatctgcac gtgcaggttt atctgcaggt attgatgcta acggaaagtt ggttgaccca
      841 acatcacgtt ggggtggttt tatgcgaaaa gtagaaacga gcaactttga agagtcaaac
      901 attcagtaca tacagttttg gttgatggat ccttttaatg gcgatgcacc tgattccagt
      961 atgtcaaata caggaacgct ttatttcaac ttgggtgata tctctgaaga tgtgttgcgt
     1021 gatggatatg aaagttatga gaatggtctt ccaacaacag caacaccggc agcatatcgt
     1081 cagaatgcat ggggccgcac acctgttgca cagaatgtac tttacgcttt tgacagagat
     1141 gaaagtacac gtaaacaaca ggatgttggt ttagatggtt tgggtaatga tgatgagaaa
     1201 ctacatttcc tcacaaactt cctcgattac attgacgcca tcgtaacgaa tcaaactgca
     1261 aaggatgcct ttcatgaaga cccttcgtca gatgatttca gatatttcag aggtgatgct
     1321 tataccaatg ccgatggaat attgcagcgt tataaaaact tcaacgggac agaaaacaat
     1381 tcaccggttg caggcagtgg aacatttgtg ccatcaagta cacaaactcc agatgcagaa
     1441 gatgcaaacc gtattaacgg tatggaagtt gacgaaaatt attttcaata taaagttgat
     1501 atagatcctt caaaattggt agtaggacaa aactatgtaa cagatgtgct ggagaatgta
     1561 gctgcattaa atcttcctga tgcatcaggt aagaccatca catggtatct gataaaaatt
     1621 ccggtgaacg atccaagtcg tgagcgcatt ggtcaaatct cagatctgaa gtccgtaaaa
     1681 tttatccgtg tttttgcaaa aggatttaac ggacccaagg tgctgcgcct tgcaaaattt
     1741 gaattcctga gaggtgaatg gaggaagtat gattataagc ttacaggtat tcttagtcag
     1801 cctgctgttt ttgatgtagg tgctgtttca ctcgaagaga acagcaaccg cgagccgatt
     1861 aattatgttt tgcctccgga ctttgagcgt gaaattgatg ttacctcaac caatcaacgc
     1921 caacttaacg agcagtcgct ttcgtttaaa gtgtgcgaac ttgctgatgg tgatatgaag
     1981 gtgtgttata aaaacacaac actcgacatg cgtaattaca aacatgtaaa aatggttatc
     2041 catgccgaat cgtttctacc acctgccaat ccaaccaacg atggtgagct ttacgcagtt
     2101 gttcgtttag gctctgactt tacagataac tactacgact accgcattcc attaaaaatg
     2161 actccttttg gaagtacaga tcctacagta atttggccta aagagaatga ttttgatatt
     2221 gatttggata aactgaccga tgctaagctg gagcgtaata atgcaggtgt tgcatttaca
     2281 gatgaatatt ctaagcttga tggcaacaat gtaattttca ttaaaggtaa tcctacattg
     2341 agcgaagtga aagttatgat gcttggattg tggaacccaa gtggtgagaa tcctgcattc
     2401 ccgccacgac caccggatga cggattggcg aagtgtgcag aagtatggtt caacgaattc
     2461 cgcatgtccg actttaatga taaaggcgga tgggcaacta taggtcgtgc gcaggtgaag
     2521 cttgccgatt tgggtaatgt ttctgtttcc ggtggctatt caactcatgg attcggaagt
     2581 ttagaaagta aagttggtga gcgtagcaaa gaagatgtga caaactatga tgtagccaca
     2641 tcacttgagt tgggtaagtt ttttccatca agtacaggtc ttacagtgcc aatgtatttt
     2701 ggttatggtg aagtattcag caatccgcaa tatgatcctc ttgatcctga tattgaattc
     2761 agccgttcaa tcagcaacat tgacgattca aaacgcaaac aaaacagaaa acgtttttcg
     2821 caggattata ctcgaagaaa gagtttgaac tttaccaatg tcaagaaaaa taagacaggg
     2881 aagaatgcat ctaagtcaag aatttatgac atagagaact ttaattttac ttatggatat
     2941 actgaagttt atcggagaaa tatcaatacg gaatatgata ttcagaaaga ctaccttggt
     3001 gcaataggtt ataattttaa tacaaaccca aaaccgatta cgcctttcag taaaatgaag
     3061 gctaagtcga agtacctacg tcctattaaa gattttaatt tcaactacat gccatcaacc
     3121 tttaatttcc gatgggatgt aaacagacac tatggcgaac ttaaagtgcg tgatgtaaat
     3181 gctgtattga atcagggtgt ttctgataat tctattcctg tgttgtatga taagcgtttt
     3241 acaatgaacc gtcagtatgg aacaagttgg gacatcaccc gcgcattgaa gtttgagttt
     3301 aatgccaaca atcaaagcaa ggttgacgag cccgatggtg ctttaaactc agaagaaaaa
     3361 cgcgattctg taagaaaaaa ctttttcaac ttaggacgta atacagatta catgcacaat
     3421 gcatctttat cgtataatct tccattaaat aagttgccga taacagattg gataagcatt
     3481 aatacgcgct acagcactac ttatcactgg acagcatcac ctttaatagt aaacacagac
     3541 aatggaaggt actatacaaa ccctgcctta ggcaacacta ttcaaaactc aaattctatt
     3601 caggttaaca acagctttaa catgacttct ttatacaata agtggaaact gtataaaaag
     3661 ttgacagcac caccaaagcc aaaaacatct gctaagaaag agccaaaggt tgatgctaaa
     3721 gcaccggatg ctgcgaaaga cagtttaaag acaaagagtg ttaaaccacc ggagaagaaa
     3781 aaagaacagg aaataaatcc tgctataaaa gcattggcaa aagttatttt cagtttaaag
     3841 aatgtttcgt ttaactggaa tgaaaccaat ggattgttgt tgcccggctt taacagacag
     3901 agtgaatggt taggtcagaa ttgggatggc accagtgcac cgggatttgc atttactttt
     3961 ggtagtcaga atcaggacat cagagaacgt gcggccaaga atggatggct tacaacagac
     4021 tcaactttca acagtcagtt tacttctacc tacacacaaa acatcacagg aagagcttct
     4081 cttgagcctt ttacaggatt taatattgat gtaaatttta accgcaatta ctctttaagt
     4141 acgacagaaa attacagagc ctacaacagt ccgcgtgcta acggtcgtta ttatgaaaca
     4201 ttggcaagag tagaaaccgg taatttcagt atcagcaatc tgatgtgggg aacatcattc
     4261 actaaagaca gaaaagatta ttcatcaacc atctttgagc ggtttgcaga aaacagaaga
     4321 atactttctg aaagattggg caatgaaaat ccaaactcat caggtgttgg cgctgatggc
     4381 tacagagatg gctacagtgg aacatcacaa gaggtagtga tactttcatt cctttcagct
     4441 tattctaaga agagtgcctc acgcatgtcg ctcgatttgt ttccatcggt accactacct
     4501 aactggacag tgcgctatga cggacttacc aaaattgcat ttgtaaaaaa tctattccag
     4561 agtgtaaacc ttaaccatgg ttatcgttct acttacaatg taaacagctt tactactgat
     4621 gtttcttatg ttcccggtgg taatgcgcgc gacatcaaca tggattttat tcctaagaga
     4681 cagatagggc aggtgagttt gagtgaacag tttagtcctt taatcggaat agacatcacc
     4741 tggaaaaaca gaaatgataa atcatcagga agtaagtcgg caacagagcg tggcggacgt
     4801 tcttccggtg gaaatttcac cacacgtttc gagtacaagc gtgatagaaa tatatcatta
     4861 acacttgcag gtattcaggt aacagaagtt aaaggacgtg agtttacatt tggagcaggc
     4921 tatcgaattc caaactttaa gtttcctttc ggactgttta agagtgttaa ctcacgcaga
     4981 tcaaacgatc tgaatacaac attagatttc tcagttcgta agaacactac cattctcaga
     5041 aaattggttg aaggtaccaa tcagccaaca gcaggtttaa ccatcatctc tattaagctt
     5101 gcttctgatt atacggtaag tgaacgactt aatttaagat tgttcttcga gaagactatc
     5161 aatacacctg tggtgtcaac atcttacccc aatgcgaata ctgcatttgg tattacaata
     5221 agatttacac tgtcgcagta ggagtcctaa aataaaataa ttgaagaggg caggctacac
     5281 cgtaacagcc tgctctttta cttccctaac ggcatttacg aagttgtcga aaagataacg
     5341 cgcatcatta gggccgggtg cagcttcggg atgatactga acagaaaaag cctttttatt
     5401 ttttacactg attccttcta tactttgatc gttcaggttt atatgtgtaa tttcaatctc
     5461 tgccttattg atagtttctt ccggaataac accgaagcca tgattttgtg atgtcacttc
     5521 tgcacgacct gtcaccaaat tttttactgg gtgattgatg ccacgatgtc cgttaaacat
     5581 tttaaatgtt tttattccac aggcttctgc aagcatctga tgacctaaac agattcccat
     5641 cagcgcttta tcggaattca gaattttttt cacggtatct accatagcag gcattacagc
     5701 aggatcgccc ggtccattgg aaaccaaata accttgcgga ttaaattttt ccatttcttc
     5761 aaaagaagtg ttcatcggaa aaacttttat gtaacaacct ctgtcagcaa gacagcgcaa
     5821 tgtgttttct tttactccta aatctattac agccacacgc aatgctgctg ctgcatcgcc
     5881 ataggtatat gctttctgag tgctgactgt gctcgataat tcaagtcctt ccatagaagg
     5941 tacttttgcc aaaagggttt tcagctttgc aagatcggtt tcttcagaag aaacaatggc
     6001 attcatagca cctttgctgc ggatgtgctg cacgatggca cgtgtatcaa catcagatac
     6061 aacaaccata ttgttttcaa taaaataatc ctgaaggctt tgatcggctt ttttgcgtga
     6121 aaaatggtca gagaaattgc gacaaaccaa tgcagatatt tttgctgacg aagattctga
     6181 ctctgcctgg cacacaccat aatttccgat gtgtacagag gtcatcacca taatctgcga
     6241 tttataactt gggtcggtaa atatttcctg atagcctgtc atgccggtgt taaaacagat
     6301 ttcaccggtg gcagttccaa ttttacctgc cgactttcca taaaataccg taccgtcctg
     6361 aagcaacaat acagcgggtg atttgtgtgc gtatttcatt tcgaaaagtt tcgcaaaagt
     6421 accattaaat cggttatcag attttttact tgtaaacagg ctgttgaaaa atcaaagctg
     6481 cccttcatcg gcaaagctgt aatagtcgtc accggcaata ataacatgat ctaagatgtt
     6541 tatttccagt aattttccgg cctgacttag ttttgatgtc agattaacat cagcctgact
     6601 tggtttagga ttgccactag ggtggttgtg acaaaggata atgcccgaag ctaaattttc
     6661 tatagccgat ttaaaaatga ttcgtgcatc aaccacagtt cccgaaacac cacctttact
     6721 gatgttttcg gatttgataa tctgattggc gcggtctaac aaaagtattc tgaattcttc
     6781 atgcggcagg tcggccagta aggctttaaa ataggctact gcatctttgc ttgaagttat
     6841 tttttgtctt tccaaagcct gtgcttgtgc gcgtctgcgt cctaattcca aagcagcaac
     6901 aatagtgata gactttactt tgcccatacc tttatacttc atcatgtctt ttacggatag
     6961 cttagaaagt tctgacagct cgttatgtgt attttttaaa atacgtttgg ccaagtcaac
     7021 agcagattcc tgacgggttc ctgaacggat gaggatagct agcaactcac tgtcggttaa
     7081 ggcttccttg cctttttcaa ggagtttctc tcttggcctg tcactttccg accatgattt
     7141 gatgttttta ggttcttcca tggtgctaag ataaggtgtt aaacaaaaaa agcccgcatt
     7201 tatagcgagc ttaaaattca aaattatgtg aatattattt taaagcacta acatatttag
     7261 tgatacttga tttcagattt gcagccttat ttttatgaat aaggttcttt tttgccattt
     7321 tatcaagcat acctgatact tccggaagca acttttcagc ttctttctta tctgtggtag
     7381 tacgcagttc tttcaaagca ttacgcgaag tttttgcctg atagcggttt ctgtcttttt
     7441 tagcaccgtt ggcgcgaatt ctttttactg ctgacttgtg atttgccata attacagttt
     7501 ttacttttta atgatggata ttaatcctta ttttttttgt agcccgtacg agaatcgaac
     7561 tcgtgtttcc tccgtgaaag ggagatgtcc taacccctag acgaacgggc caaacgtttt
     7621 tttgaggctg caaatgtata aaaacaattt acattcaaag ccttatag
//
LOCUS       DAOOOL010000115         7580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_962_length_7580_cov_45.498738, whole genome shotgun sequence.
ACCESSION   DAOOOL010000115 DAOOOL010000000
VERSION     DAOOOL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_7580_cov_45.498738"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKU82_09210"
     CDS             complement(<1..415)
                     /locus_tag="PKU82_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOZ83091.1"
                     /translation="MQKKTIVTTFFIALFALQGNAEWVKITSDNPSKSAVIVSGTPDN
                     TVMEISVSGFEKHRVKSLAGEYYTISSPEMTPRMEEGTPDLPKFALSVIIPDDGDMSL
                     NIESIEELTFQNIQIAPSKGNLYRDIKPSDVPYRFG"
     gene            596..1237
                     /locus_tag="PKU82_09215"
     CDS             596..1237
                     /locus_tag="PKU82_09215"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83092.1"
                     /translation="MAVYRFKVSFEDFEDIYREIEVTSEQTFADFYKAILSSIKFEDR
                     GTVSFFMSNDSWKKGVEISNNDKTDRDGKPVAKTDKAFLKSYIADPHQKIYFLIDCES
                     VWAFHIELIKIMAAADVKLTYPHIRKVSGDAPSQFKVKVAPVPDEGDEVLDSDTNEDD
                     NFSSTANEIAGENDYDEDDTAALEGEEGEESSDEDSEEFNDVEMGSDENYEEN"
     gene            1298..2299
                     /locus_tag="PKU82_09220"
     CDS             1298..2299
                     /locus_tag="PKU82_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83093.1"
                     /translation="MKAGDKVSFKDEKLNGVILEILQNGKVLVELEDGFPMEALQNQL
                     VVTVPFIKQQTQNDAKEDISEQNTSTENIPFDLNNNIIDFYAVPSKADRVLTGSVDHF
                     LTNGTPNLLLLIFGKRSKNQHTILYKGELKPNSYVCLSIFKRDALINLDSFYIQILIA
                     ADGEYKRPFTKDIAVELPELNQTKNTETGILSSSKKVSLVNLNVADEIQIKDLLEKFD
                     SSQPAKTSDIKKNKKQQPISDSDISKEIDLHINKLTADFQNMTNSEMLALQINTFRKE
                     MDNAIIKDYKQLIFIHGIGDGILRNAIMNETKNYSDIKVFMANQARYGTGALEIRFL"
     gene            complement(2294..4039)
                     /locus_tag="PKU82_09225"
     CDS             complement(2294..4039)
                     /locus_tag="PKU82_09225"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HOZ83094.1"
                     /translation="MMHYRFSVSNAASHFVDVELTIQTDGNETLLKLPVWRPGRYEEG
                     NFAKNIRNFRVQDINGKEVKWKKVSKSSWLLKTNKNDTIKINYQYYCNQLDAGACFVN
                     DEQIYINPIHCCFYTDANRGNACTVEIVSNFSRVATSLTQTEKNVFSAKNFDELVDSP
                     FIASDDLMHYGYTVSDCSFHIWIQGNFKPDVEKLIEDFKSFTQIQLNVMKSIPVKDYH
                     FLIQAVPHTFYHGVEHLASTVLVIGPSAKLNDRELYNELLGVASHELFHVWNIKTIRP
                     VEMQPYNYDSPNYAETGFVYEGATTYYGDLFLARCGYFNEEEYFNELNIRINKHLSNE
                     GRFNYSIAQSSFDTWLDGYVPGIPGRKTSIYDEGSLVSLMLDFLIRKYSDGKNSLDDV
                     FIELYNAFGKKNIGYTSDDYLRIASEKAGRSLETFFNDIVYKACSYQSLLEEVMLFAG
                     LELEENPSKKNAERYFGFKTNDNAAMPEVISLPIGSPAFENGLAIGDLIIGVDGQKAD
                     KNYDMQINPKENHKTGFLISRGNRIKTIEMMPASKKYFSTWKVKHISKKNAEQEQFFN
                     QWTNNLRAHHSQNSL"
     gene            complement(4041..6119)
                     /locus_tag="PKU82_09230"
     CDS             complement(4041..6119)
                     /locus_tag="PKU82_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ83095.1"
                     /translation="MIAEKKLESTPISTETVLKAFRLMCTARAMSDLYEEHATFTARY
                     VHACSRGHEAVQIALGLQLKSIDYLSAYYRDDSILLSIGMTPYELMLQLMAKRDDPFS
                     GGRTYYSHPSLRRDDMPKIPHQSSATGMQAIPTTGIALGMHYLENQNLVEYAEGEYPV
                     TVCSLGDACVTEGEVSEAFQMAVLKKLPILYFVQDNEWDISAHASEIRTMNAAEYAKG
                     FKGLETRSIDGTDFFQSYNTIKEVLDIIRTERRPFLIHAKVPLLGHHTSGVRREWYRH
                     DLEEAKKKDPLPKFIQQLEEAGFTSDELDAIKKETRTLVEDDFAKAKAADDPLPEDLF
                     KHDYAPTTVTEEKGVRQPAGKEPTVMVDCALFAVKELLQKHPECLFYGQDVGARLGGV
                     FREAATLAKTFGDDRVFNTPIQEAFIIGSTVGMSAVGCKPIVEVQFADYIWPGLNQLF
                     TEVSRSCYLSNGKWPVSCVIRVPIGAYGSGGPYHSSSVETVVLNIRGVKVAYPSTGAD
                     LKGLMKAAYYDPNPVVIFEHKGLYWSKIKGTEDARTIEPDEDYIIPFGKARIVQQAEA
                     LQDENTLTVITYGMGVYWAKAAAQEFKGRIEIIDLRTLNPLDETTIFESVRKHHRCLV
                     LTEEPLKNSFAEALSGRIQQQCFTALDAPVVAMGSENMPAIPLNSTLEFTMIPNAQKV
                     SEQMNYLLNY"
     gene            complement(6143..6907)
                     /locus_tag="PKU82_09235"
     CDS             complement(6143..6907)
                     /locus_tag="PKU82_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOZ83096.1"
                     /translation="MTEGSVESIIKDQVAEITFFHPQSNSLPGVLLRKLAATIEEAGA
                     NESAKVIVLRSKGEKAFCAGASFDELISINSESQGKEFFSGFASVINAMRKAPKFVIA
                     RVQGKAVGGGVGLACSADYTLAHVSASVKLSELAVGIGPFVVGPAVERKIGTGAFTQL
                     TVNATEWQNAQWAKEKGMFASLHDNIEDLDKAVHKLAQQLAASNPEAMKMLKQIFWKG
                     TEGWDALLMERAGMSGKLVMSDFTRIAIEKFKTKAG"
     gene            7152..>7580
                     /locus_tag="PKU82_09240"
     CDS             7152..>7580
                     /locus_tag="PKU82_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOZ83097.1"
                     /translation="MQFNIPAHLTGLTDKEVDSSRLKYGINQKKTDTRNSIFNWILDL
                     LKEPMLILLLAVSVIYLIIGDHGEAIFMFIAIVAVSAISFYQDNRSKKALEALEKLNE
                     PLSTVIRNSQVIKIPTHELVVGDLCIVEEGKMINADGKIVH"
BASE COUNT         2395 a         1453 c         1291 g         2441 t
ORIGIN      
        1 ctccaaagcg ataaggcaca tcagatggct taatatctct gtaaagattt cctttagatg
       61 gtgctatctg aatattctga aaagtaagtt cctcaattga ctcaatattc aggctcatat
      121 ctccgtcatc aggaataatc acagataaag caaactttgg caaatccggt gttccttctt
      181 ccattcttgg agtcatttcg gggcttgaaa tggtatagta ctctcctgct aaagatttta
      241 ctctgtgctt ttcaaatcct gaaacagaaa tttccattac agtattatca ggtgtaccac
      301 ttactataac agcagatttt gaaggattgt cagaggtaat cttaacccat tctgcatttc
      361 cctgtaaagc aaacaatgct ataaagaaag tggtaacgat tgtctttttt tgcatgacta
      421 aatttgttta ctcaaaatta agtctttttt tgaatactat ccgtgatttt aacaaaataa
      481 tttaccacag ccccttgtta actttgttga aatatttttt caggttagtg aataattgtt
      541 tctgaatctg aagcaaaaaa ctaattttgc acttataatt caatcttatt tatacatggc
      601 tgtatatcgt tttaaagtat cgttcgaaga ttttgaagat atttatcgtg aaatagaagt
      661 tacctcagag caaacctttg ccgattttta taaagctatt ctgtcttcaa taaaatttga
      721 agatcgcgga acagtttcat tttttatgag caatgacagt tggaaaaaag gtgttgaaat
      781 ttccaacaac gacaaaaccg atcgtgatgg aaaacctgtt gctaaaaccg acaaagcatt
      841 tctaaaaagt tatattgctg atccgcatca gaaaatttat tttttaattg actgcgaaag
      901 cgtttgggca tttcatattg aattgataaa gattatggct gctgctgatg taaaacttac
      961 ttatccgcac ataagaaaag tttcgggtga tgcaccaagc cagtttaagg ttaaagtagc
     1021 accggtacct gacgaaggtg acgaagtatt ggattcagat accaacgaag atgataattt
     1081 cagttcaact gctaatgaaa ttgcaggaga aaatgattat gatgaagatg acacagcagc
     1141 tcttgaaggt gaagaaggtg aagaaagttc tgacgaggat agtgaggagt ttaatgatgt
     1201 tgaaatgggc agtgatgaaa attacgaaga aaattaaaag tgtattttat attcttaaaa
     1261 gcacatctgt tgatgtgctt ttttaatttt tataaaaatg aaagcaggcg ataaagttag
     1321 ctttaaagac gaaaaactga atggtgtcat tcttgaaatt ttacagaatg gaaaagtatt
     1381 ggttgagttg gaagatggtt ttcctatgga agcattgcaa aatcaattgg tagttactgt
     1441 tccttttatt aaacaacaaa cacaaaatga tgctaaggaa gatatatctg aacaaaatac
     1501 ttcaacagaa aatatcccat tcgacctgaa caataatatt attgactttt atgccgtacc
     1561 atcaaaggcc gacagggtac ttacaggtag cgttgatcat tttttaacaa atggcacacc
     1621 caatctacta ttgctgattt tcggaaaacg aagcaagaac caacacacaa ttttatacaa
     1681 gggtgaatta aaacccaata gttatgtatg cctttctata tttaaacgag acgcattaat
     1741 taacttagat tcgttttaca tacaaatatt aattgctgcc gatggcgaat ataaaagacc
     1801 ttttactaaa gatattgctg ttgaattgcc tgagctgaat caaactaaaa atacagaaac
     1861 aggaattctt tcttcttcta aaaaagtaag ccttgtcaat ctgaatgttg cagatgaaat
     1921 tcagataaaa gacttattag aaaaatttga cagcagtcag ccagcaaaaa cttcagacat
     1981 caaaaagaat aaaaaacaac aaccaataag cgattctgac atcagcaaag aaattgattt
     2041 gcatataaat aaacttactg ctgattttca gaatatgacc aacagcgaaa tgcttgcgtt
     2101 acaaataaat actttcagaa aagaaatgga caatgcaatt ataaaagatt acaagcagct
     2161 aattttcatt catggcattg gtgatggtat attgcgaaat gcaataatga atgaaactaa
     2221 aaattattca gacataaaag tgtttatggc taatcaggca agatatggaa caggtgcttt
     2281 ggaaatacgc tttttataaa ctattctgcg aatgatgtgc acgaaggttg ttcgtccact
     2341 gattaaaaaa ctgttcttgt tctgcgttct ttttgctgat gtgttttacc ttccaggtcg
     2401 aaaaatattt ttttgatgca ggcatcattt caatagtctt tattctgtta cctcttgaaa
     2461 tcaaaaaacc tgttttgtgg ttttccttag gattaatctg catgtcataa tttttatctg
     2521 ccttttgccc atctacgcca ataatcaaat cacctatagc aagaccattc tcaaatgccg
     2581 gtgaacctat tggcaaacta attacttctg gcattgctgc attatcattg gtcttaaaac
     2641 caaaatatct ttctgcattc tttttagaag gattctcttc tagttccaat cctgcaaaca
     2701 acatcacttc ttcaagcaac gattgatagc tgcaagcctt gtaaacaatg tcgttaaaga
     2761 aagtttctaa acttctacct gccttttcag aagcaattct taaataatca tctgatgtat
     2821 atccaatatt ctttttacca aacgcattat acaattctat aaaaacatca tctaatgaat
     2881 tttttccgtc agaatattta cgaatcaaaa aatcaagcat cagcgacact aatgagcctt
     2941 catcataaat tgaagttttt cgtccgggaa ttcccggcac atatccatca agccaggtgt
     3001 caaaagatga ttgtgcaatt gaataattaa accggccttc attgctaaga tgtttgttga
     3061 tacggatgtt caactcatta aagtattctt cttcgttgaa ataaccacag cgtgccaaaa
     3121 acaaatcgcc ataatatgtt gtagctcctt catagacaaa tcctgtttct gcataatttg
     3181 ggctatcata attgtaaggt tgcatttcta caggtcttat ggttttaata ttccacacat
     3241 gaaacaattc atgtgatgca actcccagta actcattata caattctctg tcattaagtt
     3301 ttgccgaagg gccaatcact aaaacagtag atgccaaatg ttcaacacca tgataaaatg
     3361 tatgtggcac tgcctgaatt aaaaagtgat aatcttttac aggaattgat ttcatcacat
     3421 tcaattgtat ctgcgtaaaa gatttaaaat cttcaatcaa cttttctaca tcgggtttga
     3481 aatttccctg aatccaaata tgaaaactgc aatcagacac cgtataacca tagtgcatca
     3541 aatcatcaga agctataaac gggctgtcaa caagttcatc aaaattctta gcagaaaaaa
     3601 catttttttc tgtttgtgtg agtgatgttg ctactctgct gaagttacta actatctcaa
     3661 ctgtgcaggc attgcccctg ttggcatcag tataaaaaca acaatgtatg ggatttatgt
     3721 aaatctgttc gtcattgaca aagcaggcac cggcatcaag ctgattgcaa tagtactgat
     3781 agttgatttt tattgtatca tttttatttg ttttcagcag ccatgagctc tttgaaactt
     3841 ttttccattt cacttccttt ccattaatat cctgcactct aaaattgcgg atattttttg
     3901 caaagtttcc ttcttcgtac ctgcccggac gccagacagg aagttttaat aaagtttcat
     3961 taccgtcagt ttgtatggtt agttcaacat caacaaagtg tgaagctgca ttggatactg
     4021 aaaaacgata atgcatcatg ctaataattt aaaaggtaat tcatctgttc agatactttc
     4081 tgtgcattgg gtatcatcgt aaattctaaa gttgaattta atggaattgc aggcatattc
     4141 tccgatccca tagcaacaac aggtgcatct aatgcagtga aacattgctg ctgtatgcga
     4201 cctgaaagtg cttcggcaaa tgaatttttt agaggctctt ctgtcagcac caaacaacgg
     4261 tgatgctttc ttactgactc aaatattgta gtttcatcta atggattaag agtacgaagg
     4321 tcaataattt caattcttcc tttaaactct tgtgctgctg cctttgccca gtaaactccc
     4381 atgccgtaag taataactgt cagcgtgttt tcatcctgca aagcctctgc ctgttgcact
     4441 attcttgctt ttccaaatgg aataatataa tcttcatcag gttcaatagt gcgtgcatct
     4501 tctgtgcctt ttatcttact ccagtaaagt cctttgtgtt caaatattac tacaggattt
     4561 ggatcataat atgctgcttt catcaatccc ttcaggtcag caccggtaga tggatatgct
     4621 accttaacac ctctgatgtt tagcactact gtttcaacac ttgaagaatg atatggccca
     4681 ccacttccat aagcaccaat aggaacacgg attacgcaag ataccggcca tttgccatta
     4741 cttaaatagc aggaacggct tacctctgta aaaagttgat ttaaacccgg ccatatataa
     4801 tctgcaaact gtacttcaac aatcggttta catccaacag cactcatgcc aactgtagaa
     4861 cctatgataa atgcttcctg tatgggtgta ttaaaaaccc tgtcatcacc aaacgtttta
     4921 gctaacgttg cagcctctct gaaaacgcca ccaagtctgg ctccaacatc ttgcccgtag
     4981 aaaagacatt caggatgttt ttgaagcaat tctttaacag caaacaatgc acagtcaacc
     5041 atcactgttg gctccttacc ggcaggctga cgcacacctt tttcttctgt cactgttgta
     5101 ggtgcataat catgcttaaa caaatcttcg ggcaatgggt cgtcagcggc cttggccttt
     5161 gcgaaatcgt cttctacaag ggttcttgtt tctttcttta ttgcatccaa ctcatcagaa
     5221 gtaaaacctg cttcttcaag ttgttggata aactttggta atggatcttt tttctttgct
     5281 tcttccaaat catgtctgta ccactctctg cgaacacctg aagtatgatg tccaagtaat
     5341 ggcactttag catgaattaa aaaaggtctg cgttcagttc ggataatatc aagaacttct
     5401 ttgatggtat tgtaagactg aaaaaagtcg gtaccatcaa tactgcgtgt ttccaatcct
     5461 ttaaatcctt ttgcatattc agcagcattc atggtgcgaa tctctgaggc atgtgctgaa
     5521 atatcccact cattgtcctg aacaaaataa agtataggaa gctttttaag cactgccatc
     5581 tgaaaggctt cagaaacttc accttctgtt acacatgcat cgccaagtga acaaactgtg
     5641 acaggatatt caccttcggc atattctaca agattttggt tttcgagata atgcatccct
     5701 aaagcaattc ctgtcgtagg tatagcctgc attcctgtag cactgctttg atgaggaatc
     5761 tttggcatat catcacgtct caacgaagga tgtgaatagt atgtacgtcc acctgaaaac
     5821 ggatcatcac gtttagccat aagctgcaac atcagttcat agggtgtcat tcctattgaa
     5881 agcaaaatac tgtcgtcacg ataatatgct gagagatagt caatgctttt aagttgaaga
     5941 cctaaagcaa tctgcactgc ctcatgtcca cgcgagcagg catgaacata acgagcagta
     6001 aatgttgcat gctcctcgta caaatcgctc atggcacgtg ctgtgcacat cagcctgaat
     6061 gctttgagaa cagtttctgt tgatataggt gtagattcca attttttttc tgcaatcatt
     6121 tttctaatgt taaagaatgg ctttaacctg ctttggtttt aaatttctca atggcaatgc
     6181 gcgtaaaatc cgacataact aatttgccac tcatcccggc tctttccatt agcaatgcat
     6241 cccatccttc tgttcctttc cagaaaattt gtttcaacat tttcattgct tcagggttac
     6301 tggcagctag ttgttgtgct aatttatgta cagctttatc taagtcttca atgttatcgt
     6361 gcagagaagc aaacatgcct ttctcttttg cccactgtgc gttttgccat tctgtagcat
     6421 taactgtaag ttgtgtaaat gctcctgtac caatttttct ctctactgca ggtcccacta
     6481 caaaaggacc tatgcccaca gcaagttcgc taagtttaac agaagccgaa acatgagcta
     6541 aggtatagtc tgcagaacat gccaaaccta caccaccgcc aacagctttt ccttgcactc
     6601 tggcaataac aaactttggc gctttacgca tagcattaat tacacttgca aaaccagaaa
     6661 agaattcttt gccttgtgat tcactgttta tgcttatcag ctcatcgaat gatgctcctg
     6721 cacaaaaggc tttctcgcct ttactgcgaa gtactataac tttagctgat tcattggccc
     6781 ctgcttcttc tattgtggca gcaagtttac gtaatagcac tccgggaagt gaattactct
     6841 gagggtgaaa aaaagtaatt tcagctacct gatcttttat aattgattca accgaacctt
     6901 ctgtcatatt tgcaattgtt atttctctaa aaaattaaaa cttattcttt cgtgcaaaga
     6961 taaaaaagcc catgaagttt accagctaat tgaaagttaa tataatagct tatgacaatg
     7021 ctgttaagta cttctcaaaa tttagatgta ttctaaattt atacttctta aatttaatct
     7081 ataattgcat taaatagcaa tacaaattga cctaaacaca tctacttagg tcataaaatt
     7141 ttagccagct tatgcaattt aacattcctg ctcatttaac aggattaacc gataaggagg
     7201 ttgattcgtc acgcttaaaa tatggaataa atcaaaaaaa aactgataca agaaattcga
     7261 tttttaattg gattctggat ttgttgaaag aaccaatgct catacttttg cttgctgtat
     7321 ccgtaattta cttaattata ggagatcatg gtgaagccat ctttatgttt attgcaattg
     7381 ttgctgtttc tgccatttct ttttatcagg ataacagaag taaaaaagct cttgaagcct
     7441 tagaaaaatt aaatgaacct ctgagtacag ttatccgaaa ttcacaagtg attaaaatac
     7501 ctactcatga acttgttgtt ggagatttgt gtattgttga agaagggaaa atgataaatg
     7561 ccgatgggaa aattgtacat
//
LOCUS       DAOOOL010000116         7513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_976_length_7513_cov_53.680746, whole genome shotgun sequence.
ACCESSION   DAOOOL010000116 DAOOOL010000000
VERSION     DAOOOL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_7513_cov_53.680746"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKU82_09245"
     CDS             <1..501
                     /locus_tag="PKU82_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83098.1"
                     /translation="HLDMDKLVREVANNFYVENLRRIHNTGKDVNERLIGGGNYSTKS
                     TYVSLDQTPKKFTPIGKTGKSKFKSGKPHKSRFFPDGYKEFRQTIGAETSWVNLQLTG
                     RLKTSFIIQNTGNKYLIGFDNQYGTEISEGMEKKYSQKIWGVSIEGAAQAQAIVKRYI
                     DQHLKK"
     gene            610..1179
                     /locus_tag="PKU82_09250"
     CDS             610..1179
                     /locus_tag="PKU82_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83099.1"
                     /translation="MSKIADITTLLNLNFESTTLSSKRFQRGKFQTLAELVTREGGTV
                     PLILDNEGEGTEVELNDTYPFQVYHRILKIAPTTADEYGDGVQTKETTTLKMVVFANR
                     RVMQFETDDLITAINVGFLTHLSNVQTATLGNWFSNIDIEVTDIEIDKETVWRDEFGQ
                     TSNLPPFYQCFAFTYNVISDINTECFDLC"
     gene            1197..1739
                     /locus_tag="PKU82_09255"
     CDS             1197..1739
                     /locus_tag="PKU82_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83100.1"
                     /translation="MSAIYYPTCPDTITDPLCTPCPTKELGGIRSLVLKKSTYTFSDI
                     TNPTEWTNAICNKDVYIIPFTRGSLAVTGVEGQGFGDNSTVNDGYDYVISAFDPNYTG
                     NCEFWTDISKRRDFNVMYRTETKIHQSSNTVSIAAMAPVEEDIKTKVTWNVTVKWSQE
                     DPLCPLDIPVGIFDRCTQCS"
     gene            1746..2369
                     /locus_tag="PKU82_09260"
     CDS             1746..2369
                     /locus_tag="PKU82_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83101.1"
                     /translation="MSFCDTDIAVNCDECYELSASDCLPITIPTSLTPATVYWLICVD
                     KFDNRYDTQVTVNGSGDFIIDPDDFPTGLFNQFAGKFVFLLSVSAVDVIPVQFSVSGA
                     TYECVVVSWSVTCCNNTYVPPTACETFIDGLSATELACICDNMGELCPSGCLDATVEN
                     SDSTYLTTVASGGTLVLADTVYNFYVNGVLDQTATVASMVNQTFNIQ"
     gene            2369..3052
                     /locus_tag="PKU82_09265"
     CDS             2369..3052
                     /locus_tag="PKU82_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83102.1"
                     /translation="MPTTTINLTGQALSKTDDTNVTLTLGGTPLTALLKATSITVGWT
                     GTLADARIASAATWNAKQAALGFTPEDVANKDTDGTLAANSDTKYASQKATKTYADTK
                     VAKSSVPCEFIVACSDLTTALTTGTTKAYFRAPYAFTLTAVRASLGTAQTSGSIFTVD
                     INESGTSVLSTKLTIDNTETTSTTAATPPVISDSAIADDAIIAIDIDQVGDGTAKGLI
                     VTLIGTHAV"
     gene            3049..3834
                     /locus_tag="PKU82_09270"
     CDS             3049..3834
                     /locus_tag="PKU82_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83103.1"
                     /translation="MIINSYSFGGYTARTAAFLSATGISDATIANALNTMDLALIANG
                     LDTKMKAVYPFVGGTASTHKWNFMNPADTDAAFRLTFSGGWTHSATGAKPNGTTGYAN
                     SHFKASVNLSSINSYHQSFYSRLDTAAAERGCMGTLTGSTYWNIFPLYLDSRFYCGLM
                     ISAYMSSAEIGSKGLYLNSRTASNASATYRNSTQRATSATAPGTISNIDLYIGALNFN
                     GAPFYYTDIECAFSSIGDGLSSIDVSNFYNLIQAFNTSLARQI"
     gene            3831..4031
                     /locus_tag="PKU82_09275"
     CDS             3831..4031
                     /locus_tag="PKU82_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83104.1"
                     /translation="MRILTKEQFKLIAGEQYAKDSYFNPVLDADGDIVISEEEVEQCD
                     NEFKWVKDLPQKEFNPVVIKTI"
     gene            4057..4287
                     /locus_tag="PKU82_09280"
     CDS             4057..4287
                     /locus_tag="PKU82_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83105.1"
                     /translation="MKFSIKDYYHPTPKFWRKLGDAILYGCGSIGATGLFAFDELKSL
                     FSENELKVIIGVVLILGFAGKFLTNFFKEDSI"
     gene            4284..4610
                     /locus_tag="PKU82_09285"
     CDS             4284..4610
                     /locus_tag="PKU82_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83106.1"
                     /translation="MSEKENLTEISEKIDRLFLIIEGKGSHGIEGLVPMLQRHMKESA
                     EKFDTFKTTIVSDNIRHYKHFDERIKPLEKAQKDSARNLGIAGGLALVFGYFASELKD
                     FFGLFK"
     gene            4607..4762
                     /locus_tag="PKU82_09290"
     CDS             4607..4762
                     /locus_tag="PKU82_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83107.1"
                     /translation="MTATQLDLIKSFTSNCRQSWFRQYQFLKKVFPKLTKQELINTIR
                     HGEECDK"
     gene            4856..5323
                     /locus_tag="PKU82_09295"
     CDS             4856..5323
                     /locus_tag="PKU82_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HOZ83108.1"
                     /translation="MRITTTGTAGIYLIKGFEGFESKPYPDPATGARPYTIGYGTTVY
                     PNGTRVELTDHAIDESTALVYLSHDLKVFENGVDALTRDDLTQNQFGALVSFGYNCGL
                     QNLKVSTLLKKVNANPNDPTIAAEFLRWNKANGKVMNGLTRRRKAEADLYFSK"
     gene            5780..6163
                     /locus_tag="PKU82_09300"
     CDS             5780..6163
                     /locus_tag="PKU82_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83109.1"
                     /translation="MSAILVLTVLTHSFAHSQESKSYLIDSVQLSRANQNFVYAQGCK
                     EIKDTLFIALDTALSIINSQKLQIAAQDSAKAKCDEKYQLLQDDEAALKKQVKKEKRK
                     RTANAILFYVSVGVNVALAALILFR"
     gene            complement(6240..6533)
                     /locus_tag="PKU82_09305"
     CDS             complement(6240..6533)
                     /locus_tag="PKU82_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83110.1"
                     /translation="MTEQNLPTEPAISSSGMLGEGWLDANVHLPDELETVFISNGKGW
                     TSIGCRVYDRECGWHWAESNGVIYEEKGKIVAECESDDLDVKYWHKLPKPPFA"
     gene            complement(6600..6998)
                     /locus_tag="PKU82_09310"
     CDS             complement(6600..6998)
                     /locus_tag="PKU82_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83111.1"
                     /translation="MKKLLWLDDIRNPFENDWLVFSPIEQPFEVSWVKSYQDFCEWIT
                     KNGLPDAICFDHDLGLDEVHEKKTMSKSALKRFRKTPEYKAGYDCAKWLVEYCLDNHF
                     QIPKWNIQSANPVGKDNINGLLQSFIKNGG"
     gene            complement(7009..7275)
                     /locus_tag="PKU82_09315"
     CDS             complement(7009..7275)
                     /locus_tag="PKU82_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83112.1"
                     /translation="MDYDPELPTWTPPNQVGNCVSFIEIDPNRTFTDDFGRVRPVEFA
                     PKIIAEPKNKEEKRYNQHARISGTNVRDKTLLYSLIDQNKLQII"
BASE COUNT         2405 a         1448 c         1503 g         2157 t
ORIGIN      
        1 cacttagaca tggataaact tgtgcgagag gttgctaata acttctatgt tgagaatttg
       61 cgcagaatac acaatactgg caaagatgta aacgaaagat taattggtgg cggtaattac
      121 tcaactaaat ctacttatgt aagtttagac caaacaccta aaaaatttac gccaataggc
      181 aaaacaggaa agtctaaatt taaaagcgga aagcctcata agtcaagatt tttccctgac
      241 ggttataaag agtttcgcca aacaataggg gctgaaacat catgggttaa tcttcaatta
      301 actggacggc tgaaaacgag ttttatcatt cagaatacag gtaataaata ccttataggc
      361 tttgacaatc aatacgggac tgaaatttcg gaaggaatgg agaaaaagta cagtcagaag
      421 atatgggggg taagcattga gggtgcggca caagcgcaag ctattgtaaa acgctacata
      481 gaccaacact tgaagaaatg attacagtag aaatagaaat aagttctatt gcgggtcttg
      541 taaaagaatt gaatttaaac gcagaattag cggtaataaa agtaaacggg gaagaaataa
      601 gagttatcaa tgagtaagat tgccgacata accactttgc ttaacttaaa ttttgaaagc
      661 actacgctat cttcaaagag atttcagcgt ggtaaatttc aaactttggc agagttggta
      721 actcgcgaag gtggaactgt tccgcttatt ttagataatg aaggggaggg aacggaggta
      781 gaattaaacg acacctaccc ttttcaagtt taccaccgca ttttaaagat tgccccgacc
      841 accgcagatg aatacgggga cggagtacaa acaaaagaaa caacaacgtt aaaaatggtt
      901 gtattcgcta ataggcgagt aatgcaattt gaaacggatg atttaataac agcgattaac
      961 gtaggcttct taacgcacct gagcaatgtg cagacggcta cactcggcaa ctggttctca
     1021 aatattgata ttgaggtcac agacatagaa attgacaaag aaacagtatg gagagatgag
     1081 ttcgggcaaa cgtccaacct tcctcctttc tatcaatgct ttgcttttac ctataatgtg
     1141 atttcagata ttaatacaga atgttttgat ttatgctaat taaaaaataa aatacaatgt
     1201 cagcaattta ttatccaact tgtcccgaca ctattaccga cccgttatgc acgccatgcc
     1261 ctacaaagga gcttggaggc atacgctctt tagtgttgaa gaaaagcact tacacctttt
     1321 cggacataac aaatcctact gaatggacta acgccatctg taacaaagat gtgtatatca
     1381 ttccgtttac tcgcggttca cttgcagtaa caggtgttga agggcaaggt ttcggagata
     1441 actcaaccgt taatgacgga tatgattatg ttatttcagc ctttgaccct aattacaccg
     1501 gaaactgcga gttttggaca gacatctcta aacgcaggga tttcaatgtt atgtaccgca
     1561 ccgaaacaaa aattcaccaa tcgagcaaca ccgtaagtat tgcggctatg gccccggtgg
     1621 aggaagacat aaaaactaaa gtaacttgga atgtgactgt aaaatggtca caggaagacc
     1681 cgctttgtcc tttggatatt ccagtaggca tctttgaccg ttgcactcaa tgctcttaat
     1741 gagttatgag tttctgcgat acagatattg cggtaaattg tgatgaatgt tatgagttat
     1801 cggcaagcga ttgtttgccg ataactatac ctacatcatt aactcctgct actgtttact
     1861 ggcttatctg tgttgataaa ttcgacaaca gatatgatac ccaagtaacg gtaaacggga
     1921 gtggtgactt cataattgat cctgacgatt tcccgacagg actgtttaat cagtttgcgg
     1981 gtaaatttgt ttttctgtta tctgtttcgg cagttgatgt aattcctgtg caatttagcg
     2041 tatcgggtgc tacttatgaa tgtgtagttg tttcgtggtc tgttacatgt tgcaacaata
     2101 cttatgtgcc accaacggct tgtgaaacgt ttatagacgg actttctgca actgaattag
     2161 cgtgtatctg tgataatatg ggtgaactat gcccgtcagg gtgtttggat gccacagtag
     2221 aaaattcaga cagcacctac cttacaacag tagcaagcgg aggtacatta gtattagcgg
     2281 ataccgttta taacttttac gtgaacggag tattagacca gacggcaacg gtggcatcaa
     2341 tggtaaatca aacatttaat attcagtaat gccaacaact actataaatc taactgggca
     2401 agcacttagc aagacagacg ataccaacgt aacgctgaca cttggcggca cacccttaac
     2461 agcactatta aaagccacat caatcacagt tggttggacg ggcactttgg cggacgctcg
     2521 tatagcctct gctgcaactt ggaacgcaaa acaagcagct ttaggattta ctccagaaga
     2581 tgttgcgaat aaagataccg atggaacgtt agcagcaaat tcagacacta aatatgcaag
     2641 tcaaaaagca actaaaacat acgcagatac aaaagttgca aagtcatctg taccctgtga
     2701 atttatagtt gcctgctcag atttaacaac agccttaacc acaggaacta caaaggcgta
     2761 ttttcgcgct ccttatgctt ttactttaac agcagtcagg gcttctttag gaacagccca
     2821 aacaagcggc tcaatattta cagttgatat aaacgaaagt ggcacatcag ttctttctac
     2881 taaacttacg attgataata cggaaacaac atcgaccaca gctgcaacgc cacctgtaat
     2941 ttcagacagt gcaatagctg acgatgcaat aatagcaata gacatagacc aagtaggtga
     3001 cggaacggca aagggtttaa ttgtaacttt aatcggaaca cacgcagtat gattatcaac
     3061 tcgtattctt ttggtggcta cacagcaaga acagcagcat ttttgtcagc aacaggaatt
     3121 tcagatgcaa caattgccaa tgctttaaac acaatggatt tggcattaat tgcaaacgga
     3181 ttagatacaa aaatgaaagc cgtttatcct tttgttggtg gcacagcatc aacacataaa
     3241 tggaatttta tgaaccctgc cgacacagat gcggctttcc ggttaacttt ttcaggcggt
     3301 tggacgcatt cagcgacagg ggcaaaacca aacggaacaa caggatatgc aaattcacac
     3361 tttaaggcaa gcgtaaatct atcgtcaata aatagctatc atcaatcctt ttattcaagg
     3421 cttgacactg cggcagcgga acgggggtgt atgggtacat taacaggttc tacttattgg
     3481 aatatcttcc cactatactt agatagtcgt ttttattgcg gattaatgat aagtgcctac
     3541 atgagttcag cagaaatagg aagtaagggt ttgtatttaa attcacgaac agccagcaac
     3601 gcctcagcca cttatagaaa ttcaacacag cgagccacat cggcaactgc gcccggcact
     3661 atttctaata tagatttata tattggtgca ttaaacttta atggcgctcc tttttattac
     3721 acagatatag agtgtgcttt ttcatcaatc ggggatggtt tatcttcgat tgacgtgagt
     3781 aatttttaca atttaattca ggcttttaac acaagtttag ctcgccaaat atgagaatat
     3841 taaccaaaga acaattcaaa ttaatcgcag gagagcaata cgcgaaagac agttatttca
     3901 atcctgtttt agatgcggat ggggacattg taatatctga agaagaagtc gagcaatgcg
     3961 ataatgaatt taaatgggta aaggacttgc cgcaaaagga gtttaatcct gttgtaataa
     4021 aaactattta actttacaaa atcaaaacct cgcaaaatga aattcagcat taaagattac
     4081 taccacccaa cccctaaatt ttggcgcaaa cttggggacg ctattctata cggctgcggc
     4141 tcaatcggtg ctactggact atttgctttt gatgaattaa aatctctttt cagcgagaat
     4201 gaattaaagg taataatcgg tgtagtttta attcttggat ttgcgggaaa gttccttact
     4261 aatttcttta aagaggatag tatatgagcg agaaggaaaa cctaactgaa atctctgaaa
     4321 agatagacag acttttctta atcattgagg gaaaaggaag tcatggtatt gaaggtcttg
     4381 ttcctatgtt gcagaggcac atgaaggaaa gtgctgaaaa gttcgacacg tttaaaacaa
     4441 ctattgtttc cgacaatatc agacactaca aacattttga tgaacggata aagccgttag
     4501 aaaaggcgca aaaagacagt gcaaggaatt taggaatagc cggaggtctt gcattggtat
     4561 tcggatattt cgcatcagaa ctaaaagact tttttggact attcaaatga cagcaaccca
     4621 actcgactta ataaaatcat tcacatcaaa ctgccggcag agttggtttc gtcagtatca
     4681 gtttttaaaa aaggttttcc cgaaactaac taagcaggaa ttaataaata cgatcagaca
     4741 tggcgaagag tgtgataagt aaatcaaaaa tcgttttcgg caagcgcaag ggtggagttg
     4801 caaagcgcaa aaggaataaa cacgaaagtt tcaaggttta taaaggtcaa ggcaaatgag
     4861 gataacaacc accggcacag caggaattta cctaatcaaa ggttttgagg gttttgaaag
     4921 caaaccttat cccgaccctg caacgggcgc acgaccttat acgataggat acggaactac
     4981 tgtttaccct aacggaacaa gggtagaatt aaccgaccat gcaatagatg aaagtacagc
     5041 cttagtttat ttgtcacatg atttaaaagt atttgaaaac ggagtagatg ctttgacccg
     5101 tgacgacctt actcaaaatc aatttggggc tttggtttca ttcggttata actgtgggct
     5161 acaaaatcta aaagtaagca cgttactgaa aaaggtaaac gcaaacccaa acgaccctac
     5221 tattgcagcg gagttcctgc gttggaataa agctaacgga aaagtaatga atgggctaac
     5281 acgcaggaga aaagcggagg cagatttata cttcagcaaa tgagtatttc acaggagcag
     5341 cgcgaaaaga gagccaacaa cgaacgtgca gccggtgtga tacttctgtt tttgattttc
     5401 atttatgttt tggtaagtat ttcgggatgt tgaaaaaaaa caggagcatt aaattaataa
     5461 tacccctgcc aacgggcaag ccgtccacct tcaaaaatat gaattagtgc aaaagtacaa
     5521 aaaaatatgt taaagtatca ttatatttta ccctacgcaa tagccgtgat cttagctttc
     5581 ctgctgttta gaagctgcgg caaggtagat accctacaaa acacattaaa gcagcgagag
     5641 gcgttagaat taagtgaaaa gtactggcag gataaagctg acagtctttc aaataatatc
     5701 aaggtacgga ttgacacgct atacataact aaagtaaaac tgaaaactaa aaatgatact
     5761 atcttcatca ctcagcaaaa tgtcggcaat cttagttctg acagtgctta ctcattcttt
     5821 cgcacattca caggaaagca agtcatatct gatagatagc gttcaactta gtcgggctaa
     5881 tcagaatttc gtttatgctc agggctgcaa agaaataaaa gacaccctat ttattgcctt
     5941 agataccgcc ctttcaataa tcaattcaca gaaacttcaa atagcggcgc aggatagcgc
     6001 aaaggctaaa tgtgatgaga agtaccaact attacaagac gatgaagctg cgttaaaaaa
     6061 acaggttaaa aaagagaaac gcaagcggac tgcaaacgcg atactgtttt atgtttcagt
     6121 cggggttaat gttgctctcg cggctcttat cctattccgg taacaaaggc tttaaagaaa
     6181 tcgctcaatt cctccaacgg cagttactcc atacgcgact gtcttaaagc cggatgacgt
     6241 taggcaaaag gtggcttagg aagtttgtgc cagtatttaa catccaaatc atcgctttcg
     6301 cactccgcta ctatcttgcc tttttcttcg taaataactc cgttgctttc tgcccaatgc
     6361 catccacact ctctgtcata tactctgcat cctattgatg tccacccctt accattgctg
     6421 ataaatactg tttcaagttc atcgggtaaa tgaacatttg cgtcaagcca cccttcgcct
     6481 aacataccac ttgaagaaat ggcgggctct gtgggtaaat tttgttctgt cattttattt
     6541 ttatttatcg gttaatcaaa tttttctttt caatccgcca cttctcaagt ggttagtcgt
     6601 tagcccccat ttttaataaa tgattggagc agtccgttta tattgtcctt tcctactgga
     6661 ttggcacttt gtatattcca tttcggtatt tgaaaatgat tgtctaagca atattcgacc
     6721 aaccatttag cgcaatcata tcctgcttta tattcaggtg ttttgcgaaa tctttttaag
     6781 gcactcttgc tcattgtttt cttttcatga acttcatcta atcctaaatc gtgatcaaaa
     6841 caaatcgcat caggcagtcc gttctttgtt atccattcgc aaaagtcttg atatgatttc
     6901 acccaactaa cctcaaatgg ttgttctatc gggctaaaaa caagccaatc gttttcaaat
     6961 ggatttctta tgtcatctaa ccaaagtaat tttttcatat ttcatttatt aaattatttg
     7021 cagcttgttc tggtctatta gggagtaaag aagggtttta tctcgcacgt ttgttccgct
     7081 tattctcgcg tgttgattgt atcttttttc ctctttattc tttggctctg ctattatctt
     7141 tggcgcaaat tcaacaggtc ttacccgtcc gaaatcgtct gtaaaagttc tgttcgggtc
     7201 aatctcaata aacgatacac agtttcctac ttggtttggc ggtgtccatg ttggtagttc
     7261 ggggtcgtaa tccataccgc taaggataag gaatgttctg ccgcctaata taaatctatt
     7321 tccttttaca aattgctgca tggttacggt ttttctggaa agtatctgtt ttgcaaatct
     7381 attaatacgt tttcggtttt gtaactgccc tcataacatt tcattttaag gtttatttcc
     7441 atgtcggtag aatgaagtct ttggatagtg tcattattaa tttccatgca ctcatttatc
     7501 aactcaaaaa ttc
//
LOCUS       DAOOOL010000117         7227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1047_length_7227_cov_74.436559, whole genome shotgun sequence.
ACCESSION   DAOOOL010000117 DAOOOL010000000
VERSION     DAOOOL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_7227_cov_74.436559"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKU82_09320"
     CDS             complement(<1..769)
                     /locus_tag="PKU82_09320"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ83113.1"
                     /translation="MAMPTEGTDSLNIKLAKTKDDKTKVIVYCELMVEYIDNNLDTAF
                     MFLNPALQLAEKIKWDEGIARVKIEGGRLFWRKGSFETALKYHNDALSYFINQEDKNS
                     IAELITFIGQDYADWGKYQEALEYFVKADSIYRLNGNENKLAGIYLLYSFIYQSQGDY
                     TEALKVNYKALKIYEANGDKYGAAVSLMNIADSYKSLGNYQDALKSYFNGVKVFENNN
                     DKFNLASTYSSIAEVYKLKGNFEEALKYNSKTLAIAKG"
     gene            934..2238
                     /locus_tag="PKU82_09325"
     CDS             934..2238
                     /locus_tag="PKU82_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOZ83114.1"
                     /translation="MIQGIIFTIVLATGIFFFGKSIRRIRRNIFLGKPLDRSDNASQR
                     WMTMIRVALGQSKMTVRPIAAFFHLIVYAGFIIINIEVLEIMIDGIFGTHRILSFMGS
                     FYDFLIGSFEILAVLVIIACIVFLARRNILKLKRFWSKEMTSWPRSDANIILFTEIIL
                     MSLFLTMNACDYLLQQSGAEHYIKAGSFPISSSISNLFIGMSASSLIFIERFCWWAHI
                     IGILAFMNYLPFSKHFHILMAFPNTYYSNLNPKGRLTNLESVTNEVKLMMDPSLPPPP
                     PSDTPMRFGARDVRDLSWKQLMDAYTCTECGRCTSVCPANITGKLLSPRKIMMDTRDR
                     LDEVGKNIDKYGKEHDDDKALLNDYISPEELWACTTCNACTEACPVNIDPLSIIIDMR
                     RYLVMEQSQAPAELNGMFTKIENNQAPWQFSPADRLNWKNED"
     gene            2277..3041
                     /locus_tag="PKU82_09330"
     CDS             2277..3041
                     /locus_tag="PKU82_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOZ83115.1"
                     /translation="MADFLAANESPDILFWVGCAGSFDQRAQKITKAVAKILNSCNIS
                     FAVLGTEENCNGDPAKRAGNEFLFQMQALMNIQIMNGYNVTKIVTACPHCFNTLKNEY
                     PQLGGHYEVVHHSMLLQQLINDGKLIINGGKFLGKKIVFHDPCYLGRGNDVYEAPRDV
                     IKKLDAELFEMKRSRANGFCCGAGGAQMFKEPENGTKDVNIERTEEILEAKPDYVAVG
                     CPFCMTMLTDGVKNFNKEDSVKVLDIAELIEKANDL"
     gene            complement(3045..4871)
                     /gene="asnB"
                     /locus_tag="PKU82_09335"
     CDS             complement(3045..4871)
                     /gene="asnB"
                     /locus_tag="PKU82_09335"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ83116.1"
                     /translation="MCGIAGFIDDIADGEKILSAMLKAIAHRGPDASKMYMSQNVHLG
                     HNRLSIIDLSSNADQPMYDNGLTIVYNGEIYNYIELRAELEKNGEVFKTKSDTEVILK
                     AYQQWGEKCVEHFVGMWAFALWDQKENKLFCSRDRFGIKPFYYISNNNKFYFGSEYKA
                     LKPTPVFTNEINQYQVLRGLQMGWICYEDETYFDIIKALPAAHNLVYYRDKAEIKIYR
                     YWSLQTGVYSNLSFDEKCATFYELFSQSIKLHYRSDVPVASCLSGGLDSSAIVSMVQE
                     QHPGMSYKTFSIYYEGKGDVDERPFVNEVLKKYPALKPYYFSPSDSQVTEHFHHALYH
                     ADVPSTGSSFISQYFLMKLIKEQGIKVVLDGQGADEYLAGYKHTFYRMVADILSSGQV
                     SQALALTGIVAKNQELSSTQQLAHLGKSLLSVAKREQKLYALEYLKYYPFMGKDKHVP
                     FWLDKVDGNRTDNFLYHLLFNTSLPSLLQYEDRNSMAFSIESRVPFLDHRLVEFSFGL
                     RNEEKANGIYTKWIMRSALQNVLPPKIAWRKDKKGFVTPGEVKWLRGPLAHLIESDFN
                     KLDFLNQHKVKRIITDYKNGDNSQALLVWRLCTLAYWQKHFC"
     gene            complement(4984..5700)
                     /locus_tag="PKU82_09340"
     CDS             complement(4984..5700)
                     /locus_tag="PKU82_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ83117.1"
                     /translation="MKKLLFSVALVAMALTVNAQKFKVGLGGGFNSTWLMNKNVFDAD
                     DDLNVAMSFGGQFGLKTEYYFNDKLALELGIMRAGHNQKYDLNDGHDQLTLKLRYLDV
                     PLLLRLGGDKGGYFEFGPQFSFLSSAKDVDDDGDKHDVKGSLKGANIGLVIGFGVDVD
                     LSEQFTLTAGMRLGYGFSDVTKKYSNAIDMAQDMDDMSGATYWANFEDGTSGNFSYKT
                     TSRVYGGIHLGVLYTIPTGK"
     gene            5773..6519
                     /gene="fabG"
                     /locus_tag="PKU82_09345"
     CDS             5773..6519
                     /gene="fabG"
                     /locus_tag="PKU82_09345"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOZ83118.1"
                     /translation="MKLLENKVVLITGASRGIGRGIALTLAQHGANIAFTYLSSPEKG
                     MELENILKSHGVKAKGYQSDASQFNAAEQLINQVLTDFEKIDILVNNAGITRDNLLMR
                     MTEEQFDEVLRTNLKSCFNTTKHIQKSMLKQRSGSIINITSVVGEKGNAGQANYAASK
                     AGMIGFTKSVALELGSRNIRCNAIAPGFIETEMTGSLDPATVKGWADAIPMKRGGTVD
                     DIANAVLFFASDLSTYITGQVLHVNGGMYT"
     gene            6531..7193
                     /locus_tag="PKU82_09350"
     CDS             6531..7193
                     /locus_tag="PKU82_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ83119.1"
                     /translation="MTDFVPSLRFHFLTPFYDFLFGILLPEKKLRNTIISIIETTNND
                     SLIELGCSTGGLTLPIAKRFSSSRIYAVDIDKKALSILERKLNKVPQHKISLINASSS
                     LLPLENKSVPTIITSLLFCNLLPEEKLKTLHEIKRILTDNGHLIIMDWGKPKTIFSAA
                     GFKILQWVGGHKTTDDLKQGLFKVLVQNQGFTITEKKYINTGFGTLYFYDAMPDSQRN
                     LP"
BASE COUNT         2337 a         1395 c         1243 g         2252 t
ORIGIN      
        1 ctcctttggc aattgctaaa gtcttactat tatattttaa tgcttcttca aaatttcctt
       61 ttagtttata aacttcggca atactggaat atgttgaagc aaggttaaat ttatcattat
      121 tattttcaaa aacctttact ccattaaaat aactttttag ggcatcctga taattcccta
      181 agctcttata agaatcggca atattcatta aggatactgc agcaccatac ttgtcgccat
      241 ttgcttcata aattttcaaa gctttgtaat taacttttaa agcttctgta tagtctccct
      301 gactttgata aataaaacta tacaacaaat agattccagc caatttgttc tcattcccgt
      361 ttagacgata aatagaatct gccttgacaa aatactccaa tgcttcctga tactttcccc
      421 aatcggcata atcctgtcca atgaaagtaa ttagctctgc aatactattt ttatcctcct
      481 gattaatgaa ataacttaat gcatcattat gatacttcaa tgctgtttca aaactcccct
      541 ttcgccaaaa aagacggcca ccttcaatct ttactcttgc aataccttca tcccatttaa
      601 ttttttccgc cagttgcaat gccggattca aaaacataaa agctgtatca aggttgttgt
      661 ctatgtactc aaccattaat tcacaataaa caataacctt ggttttatca tccttagtct
      721 ttgctaactt aatgttaagt gaatctgttc cttcagtagg cattgccata gcattacctt
      781 caaaacgtat tttctcagag agaaaacaaa aagagggtaa gaaataaatc attaaaaaaa
      841 gcaggccgtt tttcacgtac gaaagataat acttaaacac aaataaattg tttggtttct
      901 tttacatttg catcaattga tttaagctca tctatgattc agggaattat atttaccata
      961 gtgctggcta ccggaatatt ctttttcggc aagagcatca gacgcatcag acgaaatata
     1021 tttttaggca aaccgcttga caggtcagac aatgcatcac aacgatggat gactatgatt
     1081 cgtgttgcct taggtcagag taaaatgaca gttcggccga tagctgcctt tttccacctg
     1141 atagtttatg ccggatttat catcatcaat attgaagtac tggaaattat gattgatggc
     1201 atttttggca ctcacagaat tttatcgttt atgggtagct tttatgattt cctcattggc
     1261 tcgttcgaga tactggcagt gttggtaata atagcatgca tcgtgtttct ggcaagaaga
     1321 aatattctga aactaaaacg tttttggagc aaggaaatga cttcatggcc acgtagtgat
     1381 gccaacatta ttctatttac tgaaattatt ttaatgtcat tatttctgac catgaatgca
     1441 tgtgactatc tgttgcagca atcaggtgca gaacactata taaaagctgg ttcatttcca
     1501 attagtagtt caatcagtaa tttatttatc ggtatgtctg cctcatcatt gatttttatt
     1561 gaaaggtttt gctggtgggc acatatcatt ggcatacttg catttatgaa ctacctgcca
     1621 ttctctaaac attttcatat tctgatggca tttccaaata cctattacag caatttgaat
     1681 ccaaaaggca ggcttacaaa tttagagagt gttaccaatg aagtaaagtt gatgatggat
     1741 ccatcattac ctcctccccc accaagtgat actcctatgc gctttggtgc aagagatgtg
     1801 cgtgacctta gctggaaaca actgatggac gcctatacgt gtacggaatg tggaagatgc
     1861 acttccgttt gtcctgcaaa catcacaggc aaacttttgt ctccaagaaa aattatgatg
     1921 gacacccgtg accgccttga cgaagtagga aaaaatattg ataaatacgg aaaagaacat
     1981 gatgatgata aagctttgtt aaatgattat atttcacctg aagagctttg ggcttgcaca
     2041 acctgtaatg cctgcacaga ggcttgtcct gtaaatattg atccgctttc aataattatt
     2101 gatatgagaa ggtatttggt aatggaacaa tcacaggcac ctgcagaatt aaacggcatg
     2161 tttactaaaa tagaaaataa tcaggcacca tggcagtttt cacctgctga ccgcctgaac
     2221 tggaaaaacg aagattgaca attaaacaaa acacgatgac attaaaagta agaaccatgg
     2281 cagatttttt agctgccaac gaatcgcccg atattttatt ttgggtaggc tgtgcaggaa
     2341 gttttgatca gcgtgcacaa aaaatcacca aggctgttgc taagatttta aactcttgca
     2401 atatttcctt tgctgttctt ggcacagaag aaaattgcaa tggtgatcct gcaaaacgtg
     2461 caggtaatga atttctattt cagatgcagg cgttgatgaa tatacaaatc atgaatggct
     2521 ataatgttac aaaaattgta actgcttgtc cgcattgttt taatacactt aaaaatgaat
     2581 atccacagtt aggtggccac tatgaagttg tgcatcacag catgttgttg caacaattga
     2641 taaatgatgg aaaactgatt atcaatggcg gaaaattttt aggtaagaaa attgtatttc
     2701 acgacccatg ttatttaggt cgtggcaacg atgtatatga agctccacgt gatgtgatta
     2761 aaaaattaga tgcagagtta tttgagatga aacgaagccg tgccaacgga ttttgttgtg
     2821 gtgcaggtgg tgcacaaatg tttaaagaac ctgaaaatgg aactaaagat gtgaatatag
     2881 aacgtacaga agaaattctt gaagctaaac cagactatgt agctgttggc tgcccctttt
     2941 gtatgactat gcttactgac ggtgtgaaaa atttcaacaa agaagattct gtaaaagttc
     3001 ttgacattgc tgaattgatt gaaaaagcaa acgatttgta aaaatcaaca gaagtgtttt
     3061 tgccagtagg ctaaagtgca aagtcgccac actaataatg cctgactgtt atcaccgttt
     3121 ttatagtcgg taattatacg tttaacttta tgttgattca gaaaatcaag tttattaaaa
     3181 tcgctttcta ttaaatgtgc taaagggcct cttagccatt tcacctcacc gggtgtcaca
     3241 aaacctttct tatccttacg ccaggcaatt ttaggtggta atacattctg taaggcacta
     3301 cgcattatcc atttggtgta tatgccattg gctttttctt cattccttaa tccaaaagaa
     3361 aattccacca agcgatgatc taaaaacggc acacgcgatt caatggagaa agccatgctg
     3421 ttacggtctt cgtattgcag cagtgatggc aaacttgtat tgaaaagaag atggtataaa
     3481 aaattatctg tcctgttgcc atcaaccttg tctaaccaaa atggaacatg tttgtcttta
     3541 cccataaacg gataatattt caggtattca agtgcatata atttttgttc acgtttagca
     3601 acacttaata aacttttacc taaatgagct aattgctgcg tagaagacag ttcctgattc
     3661 ttagccacaa tgcctgtaag tgctaatgcc tgactaactt gtcctgacga gagtatatcg
     3721 gcaaccattc tataaaaagt atgtttatat ccagccagat attcatctgc tccctgtcca
     3781 tctaacacaa cttttatccc ctgctcttta atcagcttca tcagaaaata ttgcgagata
     3841 aaagaggagc ccgtacttgg aacatcagca tgatacaaag catgatgaaa atgttccgtc
     3901 acctgactat cagatggtga aaaataataa ggtttcagag caggatattt tttcagaact
     3961 tcattcacaa atggacgctc atctacatcg cctttacctt cgtaataaat gctgaatgtt
     4021 ttgtaggaca ttcccggatg ctgttcttgc accatgctta caatggcact gctgtctaat
     4081 cctccactaa ggcaacttgc cacaggcaca tcacttctat aatgtaactt tattgactga
     4141 gaaaacaact cataaaatgt agcacacttt tcatcaaaac tcaggttgga ataaactccg
     4201 gtttgtaacg accagtaacg atagatttta atttcagctt tatcacgata atataccaga
     4261 ttatgtgcag caggcaatgc tttgatgatg tcaaaatagg tttcgtcctc atagcatatc
     4321 catcccattt gcaaaccacg caacacctga tattgattaa tttcatttgt aaaaacagga
     4381 gtaggtttta atgccttata ctccgacccg aaataaaatt tattattgtt gcttatgtag
     4441 taaaatggtt ttatcccgaa tctgtcacgc gaacaaaaaa gtttattttc cttctgatcc
     4501 cataaggcaa atgcccacat gccaacaaaa tgttcaacac atttttctcc ccactgctga
     4561 taggctttca agattacttc agtatcgctt tttgttttaa aaacctcacc attcttttcc
     4621 aattctgctc gaagctcaat gtaattgtaa atttctccat tgtaaactat agtcagaccg
     4681 ttatcgtaca tcggctgatc agcattggaa ctaaggtcaa ttatactgag tctgttatgt
     4741 ccgagatgaa cattctgact catgtacatt ttgcttgcat caggtccacg gtgtgcaatg
     4801 gctttaagca tagcagaaag aattttctcg ccatcagcaa tatcatcaat aaatccggct
     4861 atgccacaca ttttatgctt cttacaaaaa attaaaaatt tttgtcaaaa ataaataaac
     4921 catgaatcca taaaaaaacc gctccaatgt tgaagcggtc aattttataa atagttgatt
     4981 ctactatttt cctgttggaa ttgtataaag aacgcctaaa tgaataccac cataaactct
     5041 tgatgtagtc ttatagctga aattacctga tgttccatct tcaaaattag cccaataagt
     5101 agcacctgac atatcatcca tatcttgtgc catatctata gcattagaat acttcttagt
     5161 aacatcactg aaaccgtaac ccaaacgcat acctgcagtc aatgtaaatt gctcgctcaa
     5221 atcaacatca actccaaagc ctattacaag accaatattg gcacctttaa gtgatccttt
     5281 cacatcatgt ttgtcgccat cgtcatcaac atccttggca ctactaagaa aactgaactg
     5341 aggtccaaat tcaaaatatc ctcctttgtc accacccaaa cgcagtaata aaggaacatc
     5401 taaataacgt aatttcaatg taagttgatc atgaccatca ttcaaatcat acttctgatt
     5461 gtggccggca cgcataatgc ccagttcaag agccaattta tcattaaaat agtactccgt
     5521 tttcaacccg aattgaccgc caaaagacat ggcaacattt aaatcatcat cggcatcgaa
     5581 tacgttttta ttcatcagcc aggtgctgtt aaagccacca cctaaaccaa ctttgaattt
     5641 ttgtgcgttt actgtcaacg ccattgctac taaagcaaca ctgaataata gttttttcat
     5701 cacttttaaa gtttagctat atatttgaac cgcaaatgta gaactttaat tcaattgtca
     5761 aaatctaatg gtatgaaact actcgaaaac aaagttgtat tgataaccgg tgcttcgcgc
     5821 ggtattggaa gaggtattgc ccttacgttg gcacagcatg gtgcaaatat tgcctttacc
     5881 tacttgtcat cgcctgaaaa aggaatggaa ttggaaaata ttttaaaatc acatggtgta
     5941 aaagctaaag gttatcaaag tgatgcatcg cagtttaatg ctgccgagca actgattaat
     6001 caggttctta cagattttga aaaaattgat attctggtaa acaatgcagg tataacacgt
     6061 gataatctgc taatgcgaat gacagaagag caatttgatg aagtacttcg caccaacctg
     6121 aaatcttgtt ttaacaccac aaaacatatt cagaaatcaa tgcttaagca acgttcaggt
     6181 tctattataa atataacgtc tgttgttggc gaaaaaggaa atgccggaca agcaaactat
     6241 gctgcaagca aagcagggat gataggtttt acaaagtctg tagcattaga attaggctcc
     6301 cgtaatattc gttgcaatgc aatagccccg ggttttattg aaacagaaat gacaggctct
     6361 ttagaccctg caactgtaaa aggatgggct gatgcaatac ccatgaaacg cggtggaact
     6421 gttgatgata ttgctaatgc tgtcttattt tttgcatctg atttaagtac ctatataacc
     6481 ggacaggtac tgcatgtaaa tggaggaatg tacacctaat aattgatgat gtgaccgatt
     6541 ttgttccatc actacgtttt cattttctta caccatttta tgattttctt tttggcatat
     6601 tgctgccaga aaaaaaatta cgaaatacca ttatcagtat cattgaaaca acaaacaatg
     6661 atagtttaat tgaactggga tgcagcacag gcggtcttac cctacctatt gcaaaaagat
     6721 tctcctcttc acggatttac gcagtagata ttgataaaaa agcactgtcc atattagagc
     6781 gaaaactgaa taaagtacca caacataaaa tttcgctgat taatgcatct tcttctcttt
     6841 tgccattgga aaacaaaagt gttccaacta taattacaag cctgttgttc tgcaatttac
     6901 ttccggaaga aaagcttaaa actttacatg aaataaaaag aattttaact gataacgggc
     6961 atttgattat tatggattgg ggaaaaccga aaaccatttt ttcagcagct ggttttaaga
     7021 tattgcaatg ggttggtggc cacaaaacaa ctgatgattt gaaacaaggg ctttttaaag
     7081 tattggtgca aaatcaggga tttaccatta cagaaaaaaa atacatcaat acgggctttg
     7141 gaacgcttta cttttatgat gcaatgcctg attctcagag aaatctacct tagtattaaa
     7201 tattcagaac agggaataat cctgctt
//
LOCUS       DAOOOL010000118         7226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1049_length_7226_cov_64.318784, whole genome shotgun sequence.
ACCESSION   DAOOOL010000118 DAOOOL010000000
VERSION     DAOOOL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1049_length_7226_cov_64.318784"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKU82_09355"
     CDS             complement(<1..980)
                     /locus_tag="PKU82_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOZ83120.1"
                     /translation="MKIKVSLLIATAALSISTVIAQEGFRTIAGKKCQLYKVVSKDTW
                     SGLSRKYHKEIDELKAVNPGINDLKIGQIINIPIENTVNDKSENKSDTSAEIKMRKPI
                     AVINAPVKNNNKVHVIEKGETLFGIARKYDVSPNDIRKWNHLDNDGIKAGQKLIVSST
                     ESVAIKTEVKKQESKNETILQPLDDKKLEAKNNEVKNDSKLIAEASNKVIPPSPSPSE
                     MEYKPMRTEVKRSNGKSGAVIEVNESGMASWIRDGDINQSKFYALHRNAPPGTIIKVT
                     NRMNGDYVFVKVVGTLPDTGDNDKQILKMSEAAAKRIGAINEKFQVELNYG"
     gene            1126..2448
                     /locus_tag="PKU82_09360"
     CDS             1126..2448
                     /locus_tag="PKU82_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOZ83121.1"
                     /translation="MKKLFLALVLLFFATTTVNAGIVYRFNFQSEVDRGMARIFSKAL
                     QEAHEQKADLFLIHLNTYGGMLDAADSIRIAILNSKIPVVVFIDPNAASAGALISIAC
                     NRIYMRSGSSIGAATVVTEQGEAAPDKYQSYMRGIMRATAEKRNRDPRIAEAMVDPRV
                     VIPGVNDSGRVLTFTAEEALTNKYCNAIVETEADILKLENLNSDKIIEFQPSWVDNII
                     SFLIHPALSSLLILIMLAGLYFEFQAPGTIFPIAASFVAAVLYFAPLYLEGLAANWEI
                     LLFVSGVILLALEFFVIPGFGVAGIVGIICTTMGFALSMLNNKGWDFTFTSSEQITQS
                     ILISLGAMLGAVFLIFYFIGKINDSKRLSKLTLQTTQPSEHGFRAHTDIDTIQAGMRG
                     TAFTALRPSGKIMVDGRVYDAVAETGWIERQEQVEIVSVGLTCTVRTC"
     gene            2574..3440
                     /locus_tag="PKU82_09365"
     CDS             2574..3440
                     /locus_tag="PKU82_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83122.1"
                     /translation="MKNILKFSVVFLFLAVYACKDYKPEVDRLNRERDSLINTSFLKD
                     STIDSFLGDFNGIEASLDSINQIHAAITLDRKRDPEMGGTVKDRIRKNMESIASLLDQ
                     NSQRIAELTRKLKSSGVKSAQLNKMITSLNLKIAERDSMIANLNNQLGDAKIEITGLR
                     TNIETLNSTIGEREKTIEEKVNQINTAYYAIGTYKQLRDKKILNKKGGFIGIGKTKTI
                     NPDIDNSAFTKVDIRQFASVELNTKSVKIMSSHPASAYTYEKDKKKYQSLKITNPDLF
                     WSNSKYLVIVTK"
     gene            3509..3910
                     /locus_tag="PKU82_09370"
     CDS             3509..3910
                     /locus_tag="PKU82_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ83123.1"
                     /translation="MKPFVVRVYGILINKNSEVLLTHEKEGGIEFTKFPGGGLEFGEG
                     LRECLVREFLEETKQRVEVMEHFYTTDFFIESAFNPGLQLISVYYKVSCINFILPEDA
                     NDIHFFWKPLKELNGEVATFPVDRLVEKLLM"
     gene            complement(3911..6664)
                     /locus_tag="PKU82_09375"
     CDS             complement(3911..6664)
                     /locus_tag="PKU82_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOZ83124.1"
                     /translation="MNNKTDSFLTRIVDELIATVDGNNFADYTVVFPTRRAALLFKLQ
                     LAKKLNKAVLLPSVLSISDFVEHYMPLRVAEKKEVLLDLFAVYKPFFPDYDFTSFAPW
                     GEMILGDFEEINLYLVNYKDLFADLTSYHEIESVFQDDDEFKEQLKQFFELFSTDKTS
                     TIKQNFLYAWQALPKLYEKLNEVLYKNNKTTAGKAMQQLALSPETYLKNLDCSKTIIA
                     GFYALAPAEQKLFEYILNGKGKIFWDADEYYTKNTTQEAGLFFRKNFLTQNSFKWQET
                     YFKDIPKNINVTGVPLITGQFKFLADRLKTLIAQNKLNTDKTAIVLPDEKLLPLLLQS
                     LPEEIEHVNITMGFPLKETHLYRFIETLNVLLNQISETSKQKFVSTFALGNYLAHPYA
                     SLLKHDIRNLNDAGISIPLNELPENISQFLKSQITSPIFEGYIQLLTMLHDAMEPKHH
                     EKEIAGFVLNELLELRTMVIPYTEILNEQALQMLISEILDTTRIPFSGEPVKGLQVMG
                     LLETRTLDFENIFFLNVNEGSVPKSNRHHSFIPFTIRKSFGLPLLDEQDAITAYHFWR
                     LMQRAYEIDLIYNTEVNEFASGERSRFLLQLFYEMKLHFSNWQVSHQIITTPLYRYNY
                     ITPGVKRDEQLTNHLLSMVAENKLRFSASSLNSFIHCSLQYYFKEIKKIREPDDNGEE
                     IDAAVFGKILHDAVSKIYAQIIDVEFDKDNAAQLKKSISAFVKASIAQEYDKHYQGTG
                     YTVIIEKIISEYIERIIAADLKSETFSPHELETEKVVVCDIGNGINVKIKGIFDRIDK
                     VSDGYRIVDYKTGSDVVKNIKKPDELTSEPKYKVNFQLLLYCWLFLKSHSNEKVNAGI
                     YPLRRMEAGIEMLGQQPLTDHSKFEELLFALIRKILLESDFTMTTDTLRCKYCPYKDI
                     CNR"
     gene            complement(6654..>7226)
                     /locus_tag="PKU82_09380"
     CDS             complement(6654..>7226)
                     /locus_tag="PKU82_09380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOZ83125.1"
                     /translation="AEKSSVKKHSAQCHFIMGTLAPSLYQADRMTVALLNNILGGPGM
                     NSKLNLGVREKYGYTYAIESGFNSYTDTGMFHVYLATDKKFLNKSIDLAVVEMKKLTD
                     NKIGSLQLSQYKQQLKGQLALAKENKSNVVISNARSLLNFKKVFTLEDTYKKIEAVTS
                     SQLTEAAAKYLNAKSRSYSSLLYESVGHEQ"
BASE COUNT         2204 a         1281 c         1282 g         2459 t
ORIGIN      
        1 ccgtaattaa gctcgacctg aaatttttca tttattgcac ctattctttt tgcagcagct
       61 tccgacattt taagaatttg cttgtcattg tcgccagtgt caggtaatgt acctaccact
      121 tttacaaaaa cataatcgcc attcattctg ttggtgactt taattattgt gccaggaggc
      181 gcattgcgat gtaaagcata aaatttactt tgattgatat ctccatctct gatccaggat
      241 gccatgcccg attcgtttac ctcaattaca gcgcctgatt ttccattgct tcgttttact
      301 tcggttcgca tgggtttgta ttccatctca ctcggtgatg gcgatggtgg aattacttta
      361 ttacttgctt ctgcaattaa ctttgagtcg tttttaactt cattattttt tgcttctaac
      421 tttttgtcat caagaggctg aaggatagtc tcatttttag attcctgttt tttaacttca
      481 gtttttattg ctacactttc tgtacttgat acaatcaatt tctgaccggc ttttatgcca
      541 tcgttatcaa gatgattcca tttgcgaata tcattaggac tgacatcata ttttcgtgct
      601 ataccaaaaa gtgtttctcc tttttcaatg acatgaacct tgttattgtt ttttaccgga
      661 gcatttatta cagcaatagg cttacgcatt tttatttcag cagaagtatc cgatttgttt
      721 tcgcttttgt cgttaactgt attttcaatc ggaatattaa tgatctgtcc gatttttaaa
      781 tcgtttatac ccgggttaac tgcttttaac tcatcaattt ctttgtgata ttttctggat
      841 aaacccgacc aggtatcttt agaaacaact ttataaagct gacatttttt gcctgcaata
      901 gttctgaaac cttcctgtgc aattacagtc gagatgctta gtgctgctgt tgcaattaat
      961 aacgaaactt tgattttcat cccaatcact tttattttta caatactaaa aaaatcactt
     1021 cctcgtaaat gaagatgctt taataggttt taaacaaatt aattaacatc aaaacgacag
     1081 ttgtaagtag taattgtgca attttgaata ttcaacttaa atgaaatgaa aaaattattt
     1141 ttagctttag tgttattgtt ttttgcaact acaactgtta atgccggcat tgtttacagg
     1201 tttaactttc agtcggaagt ggatagagga atggcacgca ttttcagtaa agcattgcag
     1261 gaagcgcatg agcagaaggc tgatttgttt ttgattcacc tcaatactta tggaggcatg
     1321 cttgatgctg ccgacagtat cagaattgca atattaaatt caaaaattcc ggttgtagta
     1381 tttattgacc ctaatgccgc aagtgccggt gcactcatct ctattgcatg caacagaatc
     1441 tatatgcgca gtggttcaag cattggtgct gccactgtag ttactgagca aggcgaagca
     1501 gcaccggata aatatcaatc ctatatgcgt ggcatcatgc gcgcaacggc agaaaaacga
     1561 aatcgtgacc cgcgtattgc tgaagctatg gttgatccaa gagttgttat tccgggagta
     1621 aatgatagtg gaagggtgct gacttttaca gcagaagaag ccttgacaaa taaatattgt
     1681 aatgccattg ttgaaacaga agctgatatt ttaaagttag aaaatctgaa tagcgataag
     1741 attattgaat ttcaaccttc atgggttgac aacatcatta gttttttaat tcatcctgct
     1801 ttgagcagtt tattaattct tattatgctt gccggtttgt attttgaatt tcaggctccg
     1861 ggaaccattt ttccgattgc agcttctttt gttgcagcag tcttatattt tgcaccatta
     1921 tatcttgaag ggcttgctgc caattgggag atattacttt tcgttagtgg tgtgatttta
     1981 ttggctcttg agttttttgt cattccgggt tttggtgttg ccggcattgt aggaataatt
     2041 tgtactacaa tgggctttgc tttaagtatg ctcaacaaca agggatggga ttttactttc
     2101 acaagttccg aacaaataac acaaagtatt ttaataagcc ttggagccat gcttggtgca
     2161 gtgtttctga tattttattt tattggaaaa attaatgact caaagcgcct cagcaagttg
     2221 acacttcaaa caacacaacc atctgaacat ggtttcagag cacatacaga tattgacacc
     2281 attcaagccg gtatgcgcgg aacggcattt actgcactta gaccgtcagg gaaaattatg
     2341 gttgatggca gagtgtatga tgctgttgca gaaaccggtt ggattgaaag gcaggaacag
     2401 gtagaaattg tatctgttgg cctaacctgt actgtcagaa cttgttaagt gtgaatatct
     2461 ttgtttaaat cccttttatt tgatggtgtt ttaatgactg gttgcatcta tatttgcagc
     2521 tattacaatc cttcttgaat aactaactat tagaaccact aaaaattcat taaatgaaaa
     2581 acatccttaa attttctgtc gtatttctgt ttttggcagt gtatgcctgc aaagactaca
     2641 aacccgaagt agatagacta aatcgtgaac gcgactcttt gattaacaca tcttttctaa
     2701 aagacagcac tattgattca ttccttggcg acttcaacgg tattgaagca agtttagaca
     2761 gcatcaatca gattcatgct gccattacac ttgaccgcaa gcgtgatcct gaaatgggtg
     2821 gaaccgttaa agaccgcatc cgtaagaata tggagagcat tgcttcacta ctcgatcaaa
     2881 acagtcagcg cattgccgaa cttacaagaa aacttaaaag cagtggggta aaaagtgccc
     2941 aacttaataa aatgattacc tcactgaatc tgaaaattgc tgaacgtgat tctatgattg
     3001 ctaatttaaa caatcaactt ggtgacgcta aaattgaaat taccggcctg cgtaccaaca
     3061 tcgaaacgtt aaactcaact attggtgagc gcgaaaagac tattgaagaa aaagttaatc
     3121 agattaacac agcatattat gcaattggaa catacaaaca actacgcgac aaaaaaattc
     3181 tgaataaaaa aggtggattt attggtatcg gtaaaacaaa aaccataaac cctgatattg
     3241 ataattcagc attcacaaaa gttgacatca ggcagtttgc ttctgtagaa ttaaatacga
     3301 agagtgtgaa aattatgtcg tctcatcctg catcggctta tacctatgaa aaagacaaga
     3361 aaaaatatca atctttgaaa attactaatc ccgatttgtt ctggagtaat tcaaaatatt
     3421 tggtaattgt cacaaaataa ttttagagaa taataattta taaggttgat ctaagggtca
     3481 accttttttt ttgactaatt ttgaaacgat gaaaccattt gtagttcgtg tttatggtat
     3541 tctgataaat aagaattcgg aagtgttgct tacacatgag aaagaaggcg gtattgaatt
     3601 tactaaattt cccggtggcg gacttgaatt tggtgaaggt ttgcgcgaat gtttagtgcg
     3661 tgagttttta gaagaaacaa agcaaagggt agaagtgatg gagcattttt ataccactga
     3721 tttttttatt gaatcggctt tcaatcccgg cttacaactc atcagcgttt attacaaagt
     3781 atcatgcata aatttcattt tacctgaaga tgcaaatgat attcattttt tctggaaacc
     3841 tttaaaagaa cttaatggcg aagtagcaac atttcctgtt gaccgcttag ttgagaaatt
     3901 gctgatgtga ttatctgtta cagatatctt tgtaaggaca atacttacat cgtaaggtgt
     3961 cagttgtcat tgtaaaatcc gactccaata aaatttttct gattaaagca aacaacaatt
     4021 cttcaaactt tgaatgatct gttaagggtt gctgccccag catttctata ccggcttcca
     4081 ttcgcctgag tggataaatt ccggcattta ctttttcatt actgtgcgat tttaaaaaca
     4141 accagcaata aagcaaaagc tgaaaattga ccttgtattt tggctcactt gtaagctcat
     4201 caggtttttt tatattcttt acaacatcac ttcccgtctt ataatctaca attctgtatc
     4261 catcagaaac tttgtcaatg cggtcaaaaa ttcctttaat ctttacatta ataccattgc
     4321 caatatcaca aacaaccact ttttcggttt ctaattcatg aggcgaaaaa gtttcacttt
     4381 tcagatcggc tgctatgata cgctcaatgt attccgaaat tattttttca atgattacag
     4441 tatatcctgt tccctgatag tgtttgtcat attcttgggc tatggatgct tttacaaatg
     4501 ctgaaataga tttcttgagt tgggctgcat tgtctttgtc aaattcaaca tcaataatct
     4561 gagcataaat cttgctaact gcatcgtgta aaatttttcc gaatacggct gcatcaatct
     4621 cttccccatt atcatcaggc tctctgattt ttttaatttc tttaaagtaa tattgtaatg
     4681 aacaatgaat aaaactgttt agcgatgaag cagaaaatct caatttattc tctgccacca
     4741 tagatagtaa atgatttgtt aactgctcat ctcttttgac tccaggagta atgtaattat
     4801 aacgataaag cggtgtggta ataatttgat gactgacctg ccaattgctg aaatgtaatt
     4861 tcatttcata aaacaactgt aaaagaaatc ggcttctttc tccactggca aactcattga
     4921 cttctgtgtt gtaaattaaa tcaatttcat atgctctttg catcaatcgc cagaagtgat
     4981 aggcggtaat agcatcctgc tcatcgagca atggtagtcc aaaactcttt ctgatggtaa
     5041 atggaataaa tgaatgatga cgatttgatt ttggaacaga gccttcatta acattaagga
     5101 aaaatatatt ttcaaaatcc agcgttctgg tttccaacaa tcccatgacc tgaagtcctt
     5161 ttacaggctc gccactaaaa ggaattcttg tggtgtcaag tatttctgaa attagcattt
     5221 gcaaagcttg ctcattcaga atttctgtat atggtattac catagtacga agctcaagca
     5281 attcattcaa tacaaatccg gcaatttctt tttcatgatg cttaggttcc atggcatcat
     5341 gaagcatggt taacaattga atataaccct caaatatagg agaggtaatc tgtgatttta
     5401 aaaactgact gatgttttca ggaagttcat tcaacggaat gctaatgcct gcatcattca
     5461 aattacggat gtcgtgcttc agcaatgagg cataagggtg agcaagataa tttcctaatg
     5521 caaaggttga aacaaacttc tgctttgaag tttctgaaat ttgattgagt aaaacattta
     5581 gtgtttcaat aaaacgatac aaatgagttt ctttaagcgg gaatcccatt gtgatattta
     5641 catgctcaat ctcttcgggt aaagattgca acaacaaagg cagtaatttt tcatcaggta
     5701 aaacaatagc tgtcttatca gtattgagtt tattctgagc aatcaaggtt tttaaacgat
     5761 cagccagaaa tttaaattgt cctgtaatga gaggcactcc tgttacatta atgttcttag
     5821 gtatatcttt gaaataggtt tcttgccatt taaaactatt ctgagttaga aaattttttc
     5881 tgaagaaaag cccggcttcc tgagttgtat tttttgtgta atactcatcg gcatcccaga
     5941 atatttttcc ttttccattt aagatgtatt caaataattt ttgctctgca ggtgccagtg
     6001 cataaaatcc ggcaataata gtcttactgc aatccagatt ttttaaatat gtttcaggcg
     6061 acaatgctaa ttgttgcatt gctttgccgg ctgtagtctt attgttctta tataaaacct
     6121 cattcagttt ttcatataac ttcggcaatg cctgccaggc atataaaaag ttttgtttta
     6181 tagtagaagt tttatctgta ctgaacaatt caaaaaactg tttcaactgt tctttaaatt
     6241 catcatcatc ctgaaaaacc gattctattt catgatatga tgtcaggtca gcaaacaggt
     6301 ctttgtaatt aactaagtaa agattgattt cttcaaaatc tccaagaatc atttcacccc
     6361 aaggtgcaaa ggaagtaaag tcataatcag ggaaaaatgg cttgtaaaca gcaaataaat
     6421 caagaagaac ttctttcttt tcagcaactc gcagtggcat gtaatgttca acaaaatcgc
     6481 taatagataa aactgaaggc aataaaacag ctttgtttaa tttttttgcg agttgcaatt
     6541 taaaaagcaa tgcagctctt ctggtaggaa atacaacagt atagtctgca aaattatttc
     6601 catcaactgt ggcaataagt tcatcaacaa tacgtgttaa aaatgaatca gttttattgt
     6661 tcatgaccaa cgctttcata aagcagactg ctgtagctgc gcgattttgc attcagatat
     6721 tttgctgcag cttctgttaa ctgagaagaa gttactgcct caattttctt atatgtatct
     6781 tctaaggtaa atactttctt aaagtttaat aaactgcggg cattagaaat aaccacattc
     6841 gacttgtttt cttttgccaa agctagttga ccttttagtt gctgtttgta ttgactaagt
     6901 tgtaatgatc ctattttatt atcagtaagt tttttcattt ccaccacggc aaggtcaata
     6961 cttttattca ggaatttttt atcggtagcc agatagacat gaaacatacc tgtgtctgta
     7021 taggagttaa aacctgattc aatggcataa gtataaccgt atttttcacg aacacctaag
     7081 ttgagttttg agttcatacc cggcccacca agaatattat tcaacaaggc aacagtcatt
     7141 ctgtctgcct gatagagtga aggtgctaat gtccccataa taaaatgaca ttgtgcactg
     7201 tgttttttaa cagatgactt ttcagc
//
LOCUS       DAOOOL010000119         7146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1073_length_7146_cov_69.045410, whole genome shotgun sequence.
ACCESSION   DAOOOL010000119 DAOOOL010000000
VERSION     DAOOOL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_7146_cov_69.045410"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /locus_tag="PKU82_09385"
     CDS             complement(<1..2009)
                     /locus_tag="PKU82_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ83126.1"
                     /translation="MNIKDRIKIFWFSFPVQLVIMHVKKSQLLLVYWLVLFACVTQNF
                     GNRFGIPYLFLDPEYMGKVSWLAFFIIGICLGVFIMAYNISSYMLNSFRFPFLACLYK
                     TFEKYCYNNAVFPVMFILTYIGGIYYFQLKNQLLPIGIVTIQVLALLLGVGFVIFSTL
                     KYFQHTNKDIYKLFGVATHDGDHDNVKFVSPIRDTKLKRQRRRGWKVETYLTFPLKLR
                     LVRSTSHYKSFMLASVFRQNHINAAVLEMIIFLLFILLGLFRDYSVFRIPAGGSILLL
                     FTMIIMIGGVFRYWLRGWAYTALVGLFILINFLSGLEVFNFKNKAYGLNYDGAQPEYS
                     IQKMEEMLSHYRLQKDYETGLVSLNNWKKKWNDAGVEKPKLVVLNVSGGGVRSALFTF
                     NAMLELDSALNGQLLKYTQFISGSSGGLIGASYYRELYLRNKGSDKIFENRDAYLKNI
                     SKDVLNATAFSFIISDLFLNFQQFSYHGKTYLKDRAYAFEEQLNENTGHVLDKQLSEY
                     YLPELKSEIPRLVICPTVVNDGRSMVISPLPSTYLLKSKNNSNFKEAIPDGIDMMSFF
                     ENQGAGNLRYLSALRMNATFPYIMPVAQMPSEPTFQVIDAGVRDNYGVQTSVRYLIAF
                     RQWIMENTSGVVFVQIRDNNKYEQSKMRTIRSLWEKVMSPFKNLS"
     gene            complement(2055..3737)
                     /gene="ftcD"
                     /locus_tag="PKU82_09390"
     CDS             complement(2055..3737)
                     /gene="ftcD"
                     /locus_tag="PKU82_09390"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOZ83127.1"
                     /translation="MKQLIECVPNFSEGNDLTIIKQITDSIESVEGIKLLNVDPGKAT
                     NRTVVTFVGEPAAVVEAAFKGIAKAAELIDMSKHKGEHPRMGATDVCPFIPVANITMQ
                     ETAEWSKKLAARVGNKLHIPVYLYEEAQEDKKRSNLSIIRAGEYEGFFKKIKQAEWKP
                     DFGPAELPANSGATVIGARDFLIAYNVNLNTKSVKRANSVAFDIREAGRVKTDSNGKK
                     MLDDVGNEIRIPGKLKGVKAIGWYIEEYGIAQVSINITKFRETPLHLVFEESTRSAYE
                     RGYRATGSELVGLVPLQAMLDAGKYFLNKQRLSSGVSDAELIHIAVKSMGLDELSPFT
                     PEKRIIEYVMESESKPALANMRLNAFVDETASESPAPGGGSIAAYCGSLGVALATMVA
                     NLSASKKGWEERWNEFSDWADKGQKLKKQLLHLVDEDTAAFNKIMDAFKLPKTTDQEK
                     ADRKQAVAEATRYAIEVPLSVMKISLQSMELIQSMATNGNPNSVSDAGVGAMCARTAV
                     LGAGLNVRINAASYSDKAFVETVLKQAAEMESKAISNEKNILDEVNRKITGL"
     gene            complement(3886..4959)
                     /locus_tag="PKU82_09395"
     CDS             complement(3886..4959)
                     /locus_tag="PKU82_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOZ83128.1"
                     /translation="MSHLVVYSQEDINQETRTRKNETKVGENVQVLKKSDATLLERLN
                     QSDAEYVLLGLPEDIGIRANGGRGGAYSAWRPALFSLLNVQSNCFGDGSNILVLGHIN
                     FDDLMLAADGINFKTDKGLSKARELVEQIDERVFEVVKAIIQSGKEAIIVGGGHNNAY
                     PNIKAAATGLQLSGVIKTENINCINCDAHSDFRVMEGRHSGNGFRYAYEQKFLSKYAI
                     VGLHESYNAGNVLQFMMERPENFSLSFFEDIFIRENISFTQAVDKAIEHVKDNFCGIE
                     IDMDTIQNIPSSAKTSSGISTIQARQYINRVASKLNACYLHIAEAAPVLSHIKTDNKT
                     GKLIGYLIADYIKARNTFKSQKN"
     gene            complement(4962..6209)
                     /gene="hutI"
                     /locus_tag="PKU82_09400"
     CDS             complement(4962..6209)
                     /gene="hutI"
                     /locus_tag="PKU82_09400"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOZ83129.1"
                     /translation="MIVILNIKGLCGILDKEILIKRGQDLRSFEILPSAFIAVKDGLI
                     ESYGTMTEWESVKEKFSTYEMVQAEGKYLMPGWCDSHTHLVYAASREEEFVDRINGLS
                     YEDIAQRGGGILNSAKKLAAASEEDLFHSAKQRIDSMISKGTTAIEIKSGYGLSLESE
                     LKMLRVIRRIKESSPLIVKSTFLGAHAVPEKFRNNKAGYIKHIIDDMIPAVAAEKLAD
                     YCDVFCERNYFTQQETEEILNAGIKHGLIPKVHANQMSRSGGVQAGVNCNAISVDHLE
                     FVEDEEIAALKNSSTMPVVLPGAAFFLGLPLPPARKIIDAGLPLAVATDFNPGSSPSG
                     DINFMISLLCVQYKLNPEEAFNAATINAAYAMNISHLAGSISIGKRADFFITKKIPSP
                     YIIPYSFNENVVESVYVRGKKVV"
     gene            complement(6254..>7146)
                     /locus_tag="PKU82_09405"
     CDS             complement(6254..>7146)
                     /locus_tag="PKU82_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOZ83130.1"
                     /translation="SVITHSTIDSPFNCYVQDGKAKKIRVLENNERVIKNLESFVVSK
                     PEFFKFTNSEKISLNGWMITPPGFKANENKKYPVIVYLYGGPGRQTVLDKWDGSRYLW
                     HQMLAQDGFIVVSVDNRGTPGRGKEFVNSIYKNMGHNETIDQIEFAKYLRTLSYVDST
                     RIGVHGWSYGGYMTSLLMTKGADYFKVGVAVAPVINWRYYDSIYTERYLSLPAENPKG
                     YDDNSPIFFADKLKGKYLLIHGLTDDNVHFQNSAEMVKALVKSKKQFDTFYYPDQNHS
                     ISQSRWHLYEMMTEYFKKNL"
BASE COUNT         2249 a         1514 c         1125 g         2258 t
ORIGIN      
        1 ctgctcagat ttttaaatgg cgacattact ttttcccaca acgagcggat tgttctcatc
       61 ttactctgct catatttatt gttatcacgt atctgaacaa acactacacc agaagtattt
      121 tccataatcc actgcctgaa agcaatgaga taacgaacag aagtttgtac gccatagttg
      181 tcacgcacac cggcatctat aacctgaaat gttggctccg atggcatctg tgcaacaggc
      241 atgatgtatg ggaaagttgc attcattcgc aatgcagaaa gataacgaag attacctgct
      301 ccctgatttt caaaaaaaga catcatgtca attccatcag gaatggcttc tttaaaatta
      361 ctgttgtttt tacttttcag aagataggtt gatggcagag gagaaataac catggaacga
      421 ccatcgttca ccacagtagg gcaaataact aatcttggta tttcagattt taattcaggc
      481 aaataatatt cactcaattg cttatcgagt acatgcccgg tattttcatt caactgctct
      541 tcaaatgcat aagcacggtc tttaagatag gtctttccgt ggtagctgaa ttgttgaaaa
      601 ttgagaaaca aatcactgat gataaaacta aatgctgtag cattcaacac atctttagaa
      661 atatttttca gataggcatc tcgattttca aatattttat ctgagccttt atttcgcaga
      721 tacaattcac ggtaataaga agctcctatc aatccacctg aagagccact gataaattgt
      781 gtgtatttaa gtagctgccc gtttaatgca ctatctaact caagcattgc attaaaggtg
      841 aataaggcag aacgaacacc accaccactt acattcaaca caacaagttt cggtttttca
      901 actccggcat cattccattt cttcttccag ttattaaggc ttaccaaacc tgtttcataa
      961 tctttctgta aacggtagtg acttaacatt tcctccattt tctgaataga atactcaggt
     1021 tgtgcaccat cataattaag accataagct ttgttcttga aattaaaaac ttcaaggcct
     1081 gataaaaagt ttatcagaat aaacaagcca accaatgcag tatatgccca accacgaagc
     1141 cagtaacgaa atacgccacc aatcataata atcatggtaa aaagcaacag tatgctgcct
     1201 cctgcaggaa ttctgaacac actataatca cgaaataatc cgagtaaaat aaacagaagg
     1261 aaaataatca tttcaagcac agctgcatta atatggtttt gtctgaacac cgatgcaagc
     1321 atgaaagatt tatagtgtga tgtacttcga actaaacgga gtttgagagg gaaggttaag
     1381 taggtttcaa ctttccatcc gcgtctgcgc tgacgtttaa gtttagtatc gcgaattggt
     1441 gaaacgaatt ttacattatc atgatcgcca tcatgagttg caacaccaaa aagtttataa
     1501 atatccttat tggtatgctg aaaatatttt aatgttgaga agataacaaa accaacaccc
     1561 agcagaagtg ctaaaacctg aatggtgaca ataccaatag gaagtaactg attctttaat
     1621 tgaaaatagt agatgccacc aatatatgtc agaataaaca taacaggaaa cacagcatta
     1681 ttgtagcagt acttctcaaa agttttatac aaacatgcaa gaaacggaaa tctgaaacta
     1741 ttgagcatat aacttgaaat gttatatgcc ataataaaaa ctccaagaca aattccgatt
     1801 ataaagaaag caagccaact tactttaccc atgtactccg gatcaagaaa aagataggga
     1861 ataccaaagc gattaccaaa gttctgagta acacaggcaa ataaaaccaa ccaatagact
     1921 aacagcagct ggctcttctt aacatgcatt attaccaact gaacaggaaa agaaaaccag
     1981 aaaattttta ttctgtcttt aatattcatt actgcatctt caactatatt atacgcatca
     2041 cagctaattt ggttttaaag gcctgtaatt ttacggttta cttcatcaag aatgtttttt
     2101 tcattcgaga ttgctttact ttccatctca gcagcttgtt ttaagactgt ttccacaaaa
     2161 gctttatcgg aatatgatgc agcattaata cgcacattaa gtcctgcgcc taaaacagca
     2221 gttcttgcac acattgctcc tacacctgca tctgaaacag aattaggatt gccgtttgtt
     2281 gccattgact gaatgagttc cattgactgt agtgaaattt tcattaccga taatggtact
     2341 tcaattgcat agcgtgtggc ttcagcaaca gcttgttttc tgtctgcttt ctcctgatct
     2401 gtagtttttg gtaacttaaa tgcatccatt attttgttga atgctgctgt atcttcatca
     2461 actaaatgaa gtaattgttt tttaagtttc tgacctttat ctgcccaatc agaaaattca
     2521 ttccatcgtt cttcccatcc ttttttagat gcagaaagat tggcaaccat tgttgcaagt
     2581 gcaacaccta atgaaccgca ataagcagct attgatccac cacccggagc aggtgattcg
     2641 ctggcagttt catcaacaaa agcattcaac cgcatattcg ccagagcagg tttactttca
     2701 ctctccatca catattcaat aattcttttt tcaggagtga atggcgacaa ctcatcaagc
     2761 cccattgatt ttactgcaat atgaattaac tcagcatcac tgacacccga tgacaaacgc
     2821 tgcttgttca gaaaatattt tcctgcatcg agcatggcct gcaatggaac caagcccacc
     2881 aactcactcc ccgtagctcg atagccacgc tcataggcac ttcgtgtgct ctcttcaaaa
     2941 acaagatgca agggagtttc tctgaacttt gtaatattaa tcgagacctg tgcaatacca
     3001 tattcttcaa tataccatcc aatcgcttta acacctttaa gcttacccgg tattctgatt
     3061 tcattaccaa catcatctaa catttttttt ccattgctat cagttttcac acgacctgct
     3121 tcacgaatat cgaaagcaac agagtttgca cgtttaacag atttggtatt cagatttaca
     3181 ttataggcaa ttaaaaaatc tcgagcacca ataacggtag cacccgaatt tgcaggcaat
     3241 tctgcaggac caaaatcagg tttccattct gcttgtttaa tctttttaaa gaagccttca
     3301 tattcaccag ctctgatgat tgaaagatta cttcgttttt tatcttcttg tgcctcttca
     3361 tacaaataaa ccggaatatg aagtttatta cctacacgtg cagctaattt ttttgaccac
     3421 tctgctgttt cttgcattgt aatattggct acaggaataa atggacaaac atcagtggct
     3481 cccattcgcg gatgctcacc tttgtgtttg ctcatgtcaa taagttctgc tgcttttgct
     3541 atgcctttaa atgctgcttc aactactgct gcaggttcac ctacaaaagt tacaacagtt
     3601 cggttcgttg ctttacccgg atcaacattc aacaatttta ttccttctac cgattcaatg
     3661 ctatcggtaa tttgtttgat aatagttaaa tcattccctt cactgaaatt gggaacacat
     3721 tcaatcaact gcttcattta aatttgtttt aatcttttta aaaaatatgt ttgctaagat
     3781 catcaaaatt actctgaaaa catattcgtt aacgaaaagc ataaaaagtt ttaaacgaaa
     3841 ttttattaat tgtaccctga gcaaatgtca ttgggtataa tcagcctaat tcttttggct
     3901 tttaaacgta ttgcgtgctt tgatataatc tgcaataaga tagccgatta acttacctgt
     3961 tttattgtca gtcttaatgt gtgaaagcac gggagcagct tcggcaatat gtagataaca
     4021 agcatttaac tttgaggcta cgcgattaat gtactgcctt gcctgaatgg tactgatgcc
     4081 acttgaagtt ttagcacttg aaggaatatt ctgaatggta tccatatcaa tttcaatacc
     4141 gcagaagttg tccttaacat gttcaatggc tttatcaact gcctgagtaa atgaaatatt
     4201 ttctctgata aaaatatctt caaaaaaaga cagtgaaaaa ttttccggtc tctccatcat
     4261 aaactgaagc acattacctg cattatagct ttcatgcaaa ccgacaatgg catacttact
     4321 gagaaacttt tgctcataag catatctaaa tccattgccg ctgtgacggc cttccatgac
     4381 tctgaaatcg gaatgtgcat cacaattgat acagtttata ttctcagttt taatgactcc
     4441 actcagttgc aggcctgttg cagcagcctt aatattagga tatgcattgt tatgccctcc
     4501 accaactatg atggcttctt tgccggattg aattatggcc tttacaacct caaaaactct
     4561 ttcatcaatc tgttcaacaa gttctcttgc tttgctaagt cctttatcag ttttaaaatt
     4621 aatcccatcg gcagcaagca tcagatcatc aaaattaata tgaccaagaa ctaaaatatt
     4681 actaccatcg ccaaaacaat tactctgcac attcaacaag ctgaacaatg caggtctcca
     4741 tgcactatat gcgccaccac gacctccatt agctctgatt ccaatatctt caggcagacc
     4801 caataataca tattcagcat cagattgatt taatcgttct agtaatgtag catcactttt
     4861 tttgagcacc tgcacattct cgcccacctt agtttcattc ttgcgggtgc gtgtttcctg
     4921 attaatatct tcctgactgt aaacaacaag atgactcata tttacacaac ttttttacct
     4981 cttacataaa ccgattcaac cacattttca ttgaatgagt agggtatgat ataaggcgat
     5041 ggtatttttt tagtaataaa aaaatctgct cttttaccaa tggaaatact acctgcaaga
     5101 tgactgatat tcatggcata ggcagcgttg atagtggcag cattaaaggc ttcttcagga
     5161 tttaatttat actgaacaca aagaagactt atcataaaat ttatgtcgcc actgggcgag
     5221 ctaccgggat taaaatccgt tgcaacagcc aatggcaagc ctgcatcaat aattttgcgt
     5281 gcaggtggca atggcaaacc taaaaagaat gcagcaccgg gtaatacaac aggcattgtt
     5341 gaactatttt taagcgctgc aatttcctcg tcctctacaa attcaagatg atcaacactg
     5401 atggcattgc aatttacccc tgcctgaaca ccacctgaac gactcatctg attggcatgt
     5461 acctttggaa tcaatccatg ttttatgcct gcattcaaaa tttcttctgt ttcctgctgc
     5521 gtaaaataat ttcgctcaca aaatacatca caataatcag ctaacttttc tgcagcaact
     5581 gccggaatca tgtcatcaat aatgtgtttt atatatcctg ctttattgtt tctgaacttt
     5641 tcgggcactg catgagctcc taaaaaagta gattttacaa tcagtggtga actttcttta
     5701 atacgcctaa ttaccctaag cattttcagt tcactttcaa gacttaagcc atagccgctc
     5761 tttatctcta tagctgtagt ccctttggat atcattgaat caatacgttg ctttgctgag
     5821 tgaaacaaat cttcctctga tgcagcagca agtttttttg ctgaattaag aatgcctcca
     5881 cctcgttgtg caatatcttc ataagacaat ccattgatgc ggtcaacaaa ttcttcttcg
     5941 cgcgaagcag cataaacaag atgtgtatgt gaatcgcacc aacccggcat cagatattta
     6001 ccttctgcct gaaccatttc gtaagtacta aatttctctt tcacagattc ccattcagtc
     6061 atggtaccat agctttctat taacccatct tttacagcta taaaagcaga aggcaagatt
     6121 tcaaaactgc gtaaatcctg tccacgtttg atgagtatct ctttatcaag aattccacaa
     6181 agacctttaa tattaagtat tacaatcaca atttttaagg ttgattttaa ctgaggtaaa
     6241 actataagga acgttataaa tttttcttaa aatattctgt catcatttca tacaaatgcc
     6301 aacgagattg gctaatggaa tggttctgat cagggtagta aaaagtatca aactgctttt
     6361 tacttttcac taatgccttt accatttctg cactgttctg aaaatgtaca ttatcatcag
     6421 ttaatccgtg tatgagcaaa tatttacctt tcaatttatc agcaaaaaat ataggtgagt
     6481 tatcatcata tcccttggga ttctctgcag gtaatgacaa atatctttca gtataaatag
     6541 aatcgtaata acgccaattg ataacaggcg ctacagctac accaacttta aaataatcag
     6601 caccctttgt cataagtaaa gatgtcatat agccaccata actccagcca tgaacaccaa
     6661 tgcgggttga atcaacataa gataatgtac gaagatactt tgcaaattca atctgatcta
     6721 ttgtttcatt atgacccatg tttttataaa tactattgac aaattctttg cctctaccag
     6781 gtgttcctct gttgtcaact gatacaacaa taaatccatc ttgtgcaagc atctgatgcc
     6841 acaagtatct tgacccatcc catttatcga gcacagtttg tctgcccggt ccaccatata
     6901 aatatacaat aacaggatac tttttatttt cattggcttt aaaacccggt ggtgttatca
     6961 tccatccatt taaactaatt ttctctgaat ttgtaaactt aaaaaactca ggcttggaaa
     7021 caacaaacga ttccagattt ttgattaccc tttcattatt ctccaacact cttattttct
     7081 ttgctttgcc atcctgaaca taacaattaa aaggactatc tatggtgcta tgggtaataa
     7141 cgctat
//
LOCUS       DAOOOL010000120         7043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1097_length_7043_cov_71.061820, whole genome shotgun sequence.
ACCESSION   DAOOOL010000120 DAOOOL010000000
VERSION     DAOOOL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1097_length_7043_cov_71.061820"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PKU82_09410"
     CDS             <1..1169
                     /locus_tag="PKU82_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOZ83131.1"
                     /translation="YNVEVSAAGYFTKTINGVSLTNGNVTQLDVQLSPLASTSLSGQV
                     VEAVTGNPIPNAMVTIQDSALVYNTTADASGNFTIPSMVIGNYQVVAGSWGHRTTCFN
                     SSIMLNPAPLVITLNKGYYDDFSFDFGWTITGTTQNLWERGVPHGTPAVFGPGFANPD
                     ADDQTDCLDQAYVTDNNPNASSSGSNDVDDGYTLLTSPVFDPTYLTNPTINYVRWFYN
                     AGGNSNPNDSLIIRVSNGTTNVVLETVRASTPGMGTWVPQSFNLTGLIPITSTMQISV
                     YTADQAMSGHVVEGGFDKFEVTGTVGISTLISATNTLSSSPNPFNNESRLTIGENGGT
                     LTITDITGKMVETLKVHKNEQILIGKNFQKGIYLVKLVSDQGVISSIKLVKTGE"
     gene            1343..1654
                     /gene="rplU"
                     /locus_tag="PKU82_09415"
     CDS             1343..1654
                     /gene="rplU"
                     /locus_tag="PKU82_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOZ83132.1"
                     /translation="MYAIVNILGQQFKVEEKQQIFVHRLEGAEGEEVKFNDVLLVDNG
                     GNIKVGKPSLSNASVSAKIISHVKGDKVLVFKKKRRKGYKKMNGHRQYMSKIEINSIN
                     A"
     gene            1675..1938
                     /gene="rpmA"
                     /locus_tag="PKU82_09420"
     CDS             1675..1938
                     /gene="rpmA"
                     /locus_tag="PKU82_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOZ83133.1"
                     /translation="MAHKKGEGKVKNNRESHSKRLGIKIFGGQHAIAGNIIVRQRGTK
                     HNAGEGVGMGKDHTLFALVDGKVVFKKKTDNKSYVYITPVAQA"
     gene            2031..3083
                     /gene="lpxK"
                     /locus_tag="PKU82_09425"
     CDS             2031..3083
                     /gene="lpxK"
                     /locus_tag="PKU82_09425"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOZ83134.1"
                     /translation="MKSLRMLLLPFSWLYGMVVMLRNFLFDAGLLKSRTVNLPVICIG
                     NLNTGGTGKTPHTEYLLSALSNYKTAVVSRGYGRNTSGIAEVLVDSQANDTGDEPLQI
                     KRNFPTVPFIVSEKRNDGIDYLLKHHPNTEVILLDDAFQHRSVKAGMNLLLTQYNDLF
                     TNDCLLPAGNLREPALSASRADVIIVTKCPSTITAEQKKSIISELPKKPVFFSSFNYD
                     NLQSLNSNEKLNLTDLYEYEILLITGIASNKNLLDFLSTHSRKIKEISFKDHHNYCAK
                     DFENITAIFNTLASEKKIIVVTEKDAVKFNSSELKPLTNNLPVYSIPVTVVINENEIK
                     FLDIVKSYVGKNQSNG"
     gene            3055..3864
                     /locus_tag="PKU82_09430"
     CDS             3055..3864
                     /locus_tag="PKU82_09430"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOZ83135.1"
                     /translation="MLEKIKATAEFISKTTGFSPEIGIILGSGLGGLVKEIEIVKELN
                     YSDIPGFPVSTVKGHGNKLIFGTLNGVKVVAMQGRFHFYEGYSMQEVAMPVRVMKFLG
                     IKNLILSNASGGVNPKYKVGDLMILDDHINLMGTNPLIGPNNDDLGPRFPDMGEPYDK
                     TMIKNAKAIADKHGFTCHTGIYAAVTGPTFETRAEYKYIKFIGADCVGMSTVPENIVA
                     RHMNLPVFAISIITDLGGSDEPEFITHEEVLKVANAAEQKMTVIIKELIAQ"
     gene            3963..>7043
                     /locus_tag="PKU82_09435"
     CDS             3963..>7043
                     /locus_tag="PKU82_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOZ83136.1"
                     /translation="MQKVQAQFWLPIGSGVNDNVYAMTKDTVNNILYIGGRFTDASGV
                     TAIRIASWNGTTYTAIGNGFDSNVFALYYDHSNNTLYAAGSFLFSGINPITRIAKWDG
                     TQWLPLGTGCNDEIYALTGDANGNIYAGGIFTTAGGVSTERIAKWNGSTWSALGSGIG
                     SGSNYVEALTFYNGDLIAGGQFGVAGGVSVSGIARWNGTNWFDLNGGVSGLSMRVSAF
                     KEVNGELICGGTFTAAGGVSSNSVAKWNGAAWLAIPGGITGGQAKVKSLGYLFNTLYV
                     GGNFSLAGGNAVSNITAYDGTTWADLSGGTNNQVDALLNYGNEMYVGGAFTMAGGNPA
                     TYIAKYRTTCLTNASITTTQTSCFGQCNGTATVTATGNAPFTYQWSSTPVQTDSTAAD
                     LCAGNYTVTITDNIGCSITQPVTITEPAQFAVTFSSNNPTCFGLCDGNALASNNGQGT
                     VTYNWNTIPAQTTQTATGLCEGTYSVTLIDSAGCMITDSVTIANPAPNTLTLTSSNPS
                     CYNNCDGIAAVASTGNAPFQYLWNTIPAQQTDTATGLCAGVYYITVTDSTGCMATDSI
                     EIVNPAAATLQFSTTTATCFGSCNGSASVVSSGTSPFTYLWNTTPVQTTDTATNLCAG
                     TYFVTVTDSNLCAVTDSVVITEPVANLISFTAQLPACVSSCNGLLTATSTGTAPFIYQ
                     WSNGDSISTIDSLCTGLYTIIISDSIGCTVTDSISLNPAPPLPITFTTQFAGCNGVCT
                     GAVSAAITGINPISYIWSTGDTIASIDSLCQGIYTVSITDSLGCVVTDSAQIISQPVL
                     LNPIATAPTCAGQCNGLASINPSGVAPFQWQWSTGDTTSLSIDSLCAGVYYITITDSV
                     GCVGSDSVTIVDPPAITYSASHTDATCANLCNGISTITATGNGTLTYLWNTVPPQTDS
                     TNTNLCFGYTTYIITDTNNCSVTDSVLIFEPSPIFIGNNFLGITCNGNCDGFVRALPS
                     GGTPGYTYLWSNGVTFDSIFNTCAGTYTVTVTDANLCTAVDSFTFVEPDPIVINF"
BASE COUNT         2253 a         1326 c         1355 g         2109 t
ORIGIN      
        1 gttataatgt tgaagtttct gcagcaggtt attttaccaa gacaattaac ggtgtttcgc
       61 tcactaatgg aaatgtaaca caacttgatg ttcagttgtc accacttgct tctacatcac
      121 taagcggaca ggtagttgaa gctgtaacag gaaacccaat acccaatgca atggtcacca
      181 ttcaggatag cgcattggtg tataatacaa cagccgatgc ctcaggcaat tttacaattc
      241 cttcaatggt aattggcaac tatcaggttg tagccggaag ttggggacat cgcactacat
      301 gtttcaactc aagcattatg cttaaccctg caccattggt tataactttg aacaaaggtt
      361 attatgatga cttttctttt gactttggct ggacaataac aggcactact caaaatcttt
      421 gggaaagagg cgtaccacat ggaacacctg cagtattcgg accgggcttt gctaatcctg
      481 atgctgacga tcagacagac tgtttggatc aggcttatgt taccgataac aatcctaatg
      541 catcttcttc aggatcaaac gatgttgatg atggctatac attattgaca tctcctgttt
      601 ttgacccaac ttatctcacc aatccaacca taaattatgt tcgctggttt tacaatgccg
      661 gtggaaattc aaatccaaat gacagtttaa ttattcgtgt ttcaaatgga actaccaatg
      721 tagttttaga aacagttcgt gcttctactc ccggaatggg aacatgggtg ccacagtcat
      781 tcaacttaac tggtttaatt cctattacca gcaccatgca gattagtgtt tacactgccg
      841 atcaggcaat gtcaggacat gtggttgaag gcggttttga taaatttgaa gtaacaggaa
      901 cagttggcat ttcaacacta atttcggcaa caaacacttt gtcgtcaagc cctaatcctt
      961 ttaacaatga atctaggttg acaattggcg aaaatggtgg cactttgacc attacagata
     1021 ttaccggcaa aatggtggaa acgctaaaag ttcataaaaa tgaacagatt ttaattggca
     1081 agaacttcca aaaaggcatt tatcttgtga aattggtatc tgatcagggt gttatttcaa
     1141 gtattaagtt agttaaaacc ggagagtaaa tttttatatt atattgataa tcaaatattt
     1201 ttattctatt aatagagtat aatcagcgtc attttccggc cgattaacca aattattgaa
     1261 gttgaaaata aatttaataa aggatttgaa aatatagtaa aaaaaactac ttttgccgtc
     1321 cgtttgtaaa aacacattaa tcatgtacgc aatagtaaac atattaggac aacagtttaa
     1381 agttgaagaa aagcagcaaa tatttgtaca ccgtttagaa ggtgctgaag gcgaagaagt
     1441 aaaattcaac gatgtattat tagttgataa tggtggcaac ataaaagttg gcaagccttc
     1501 tttatcaaac gcaagtgtat ctgcaaaaat catcagtcat gtaaaaggtg acaaagttct
     1561 ggtgtttaag aaaaagagaa gaaaaggata taagaagatg aatggtcatc gtcagtatat
     1621 gagtaaaatt gaaatcaatt ctattaacgc ataaaaaact aaacatattt agtcatggca
     1681 cataaaaaag gcgaaggtaa agtaaagaac aaccgcgagt cgcacagtaa acgtctcgga
     1741 attaaaatat ttggtggaca acatgctatt gcaggcaaca tcattgttcg tcagcgtggc
     1801 accaagcata atgcaggtga aggtgtaggt atgggaaaag atcatacttt gtttgcattg
     1861 gttgatggca aagttgtttt caagaaaaaa actgataaca aatcatacgt gtacattacc
     1921 cctgtagcac aggcttaatt taaaatatta aattgttaaa agcctaccct ttaacacggg
     1981 taggctttct tatttttata aaatttcttt tctatcaata cttttgttca atgaagtcat
     2041 tgcgcatgtt actccttcct ttctcgtggc tgtacggaat ggttgtaatg ttgcgtaatt
     2101 ttctatttga tgccggatta ctaaaaagca ggactgtaaa tctgcctgta atatgcattg
     2161 gaaacctaaa cacaggtggc acaggcaaaa caccacatac agaatattta ctttctgcat
     2221 tgagtaatta caaaacagcc gttgtttctc gcggatatgg acgtaacaca tcaggcattg
     2281 ccgaagttct ggttgacagt caagccaatg acaccggtga tgaaccatta cagattaaac
     2341 gcaattttcc aactgtacct ttcattgtca gtgaaaaaag aaatgatggc attgattatc
     2401 ttttaaaaca tcatccaaat acagaagtaa tcttacttga cgatgccttt cagcatcgca
     2461 gtgtaaaagc cggaatgaat ttattgctga cacaatacaa tgatctgttt acaaatgatt
     2521 gcttattacc tgcaggtaat cttcgggaac cggcattgtc tgcatcaaga gcagatgtaa
     2581 ttatagttac caaatgccca tcaacaataa cagcagaaca aaaaaaatca atcatcagtg
     2641 aattacctaa aaaacctgtc ttctttagtt catttaacta tgacaatctt cagtcgttaa
     2701 atagtaatga aaaactgaat ttaacagacc tgtatgagta tgaaattttg ttaataacgg
     2761 gaattgcatc caataaaaat ttactcgatt ttctgagtac tcatagcaga aaaatcaaag
     2821 aaatatcctt taaagaccat cacaattatt gtgcaaaaga ttttgaaaat atcactgcca
     2881 tttttaatac tttagcctct gaaaaaaaaa taattgtagt tacagaaaaa gatgctgtaa
     2941 aattcaacag cagtgaatta aaaccactca caaataatct tcctgtatat tcaatacctg
     3001 taacagttgt catcaatgaa aacgaaataa aatttttaga tatagtaaaa tcgtatgttg
     3061 gaaaaaatca aagcaacggc tgaatttatc agcaaaacca ccggctttag tccggaaata
     3121 ggaataattt taggatctgg tcttggagga ttagttaaag aaattgaaat tgtaaaagaa
     3181 ttaaactatt cagacattcc cggatttcca gtgtcaacag ttaaaggtca tggtaacaaa
     3241 cttattttcg gaacattaaa cggagtaaag gtagtagcga tgcaaggtcg cttccatttt
     3301 tatgagggat actccatgca ggaagttgca atgcccgtga gagtgatgaa atttcttgga
     3361 ataaaaaatc tgattctttc caatgcaagt ggtggtgtta atccaaaata taaagtaggc
     3421 gatttgatga ttttggatga ccacataaat ctgatgggta ccaacccgtt gataggacct
     3481 aacaatgatg atttaggccc acgctttccg gatatgggcg agccttacga taaaacaatg
     3541 attaaaaatg caaaagccat tgcagacaaa catggcttta cctgccatac aggaatttat
     3601 gctgccgtaa cagggccaac gtttgaaaca cgtgcagaat acaaatacat taaatttata
     3661 ggtgcagatt gtgtgggcat gtcaactgtt ccggaaaaca ttgttgctcg tcacatgaat
     3721 ctgcctgttt ttgctatctc tataatcacc gacttaggcg gaagcgatga accggagttt
     3781 attactcacg aagaagtttt aaaagttgct aatgcagcag aacagaaaat gaccgtaatt
     3841 atcaaagaac ttattgcaca ataattttta tttcaattta tttcagaaga agaatcatga
     3901 gaaaaagaat tcaatttaat atctctttat ttagttttat catttgcttt tttatttgca
     3961 cattgcaaaa agtacaagca caattttggt tacccattgg ctcaggcgta aacgataatg
     4021 tttatgcaat gaccaaggat accgttaaca acattcttta catcggtgga agatttacag
     4081 atgccagtgg cgttactgcc atacgcatag catcatggaa tggaacaacc tatacagcta
     4141 taggaaatgg atttgacagt aatgtatttg cgctatatta tgatcatagc aacaatacac
     4201 tttatgcggc aggctcgttt cttttttcag gaataaatcc gataacacgt attgcaaaat
     4261 gggatggcac acaatggcta ccattaggca caggctgcaa tgatgagatt tatgcactta
     4321 ccggagatgc taatggaaac atttatgccg gtggaatatt tacaacagcc ggtggcgtaa
     4381 gcactgagcg tattgctaaa tggaatggca gtacatggag tgcattaggt tctggcattg
     4441 gtagtggctc taactatgtt gaagcattga ctttttacaa tggtgattta attgcaggtg
     4501 gacagtttgg tgtagcaggt ggtgtatcag taagcggcat tgcacgatgg aatggcacca
     4561 actggtttga cctgaatggt ggcgttagtg gattgtctat gcgagtgtct gctttcaaag
     4621 aagtaaacgg tgagttgata tgtggtggaa cttttactgc tgcaggtggc gtttcgtcca
     4681 atagcgttgc taaatggaat ggtgctgctt ggttagcaat tcccggaggt ataaccggag
     4741 gacaagctaa agtaaaatca cttggatatt tatttaatac actttatgta ggtggtaatt
     4801 tttcgcttgc aggcggaaat gctgtttcaa atattacagc ttatgatggt actacatggg
     4861 ctgaccttag cggtggaaca aacaatcagg ttgatgcatt attaaattat ggcaatgaaa
     4921 tgtatgttgg aggtgctttc actatggctg gaggaaatcc ggcaacctac attgccaaat
     4981 acagaacaac atgtttaacc aatgcatcca tcactacaac acaaacttct tgttttggtc
     5041 agtgcaatgg aacagcaaca gttacagcaa caggaaatgc accctttacc tatcaatggt
     5101 catctactcc tgttcagact gattcaacag cagctgatct ttgtgcagga aattatacag
     5161 ttacaattac tgataatata ggttgcagca ttacacaacc tgtaacgatt acagagcctg
     5221 cacagtttgc agttaccttt tcttcaaaca accctacctg ctttggattg tgcgatggca
     5281 atgcattagc atcaaacaac ggacaaggta ctgtaacata caactggaat accattcctg
     5341 cacaaactac acaaactgct acaggtcttt gtgaaggcac ttattcagtt acgcttattg
     5401 actcagcagg ttgtatgata acagattcag ttactatagc caaccctgct cctaacacac
     5461 ttacattaac atcatcaaac ccttcatgtt ataataactg tgatggcatt gccgcagtag
     5521 catcaacagg aaatgctcct tttcaatatt tgtggaatac aatcccggca caacaaaccg
     5581 atacagcaac agggctttgt gcaggtgttt actacattac cgttactgac agtacaggtt
     5641 gtatggctac agattcaatt gagattgtca atcctgcagc agcaacatta cagttcagca
     5701 ctacaacagc aacttgtttt ggaagttgca acggttctgc atcagttgtt tcgagcggaa
     5761 cttcaccatt tacttatttg tggaacacaa ctcctgttca aaccacagat acggcaacaa
     5821 atttatgcgc aggaacctat tttgttactg ttaccgacag taacttgtgt gctgtaaccg
     5881 actccgtagt gattacagag cctgtggcca atctgatttc ttttacagct caacttccag
     5941 catgtgtcag ttcttgtaac ggcttgttaa ctgcaacatc aaccggaaca gccccattta
     6001 tttatcaatg gtcaaacggt gattcaataa gcactattga ttcactctgc acaggtcttt
     6061 ataccattat cataagtgac agtattggct gcacagtaac agattctatt tcactaaacc
     6121 ctgctcctcc tcttccgatt acattcacaa cacaatttgc aggatgcaac ggtgtgtgca
     6181 ctggcgcagt aagcgcagca attacaggca tcaatccaat aagctatatt tggtcaacgg
     6241 gtgataccat tgcttcaatt gattcacttt gtcagggtat ttatactgta agcattaccg
     6301 atagtcttgg atgtgttgta acagactctg cacaaattat cagtcagcct gttttactaa
     6361 atccaattgc aacagcacca acctgtgcag gacaatgcaa tggattggct tcgataaatc
     6421 catcaggcgt agcaccattt caatggcaat ggtcaacggg cgatacaaca tcactttcta
     6481 ttgacagttt atgtgcaggt gtatattaca tcaccataac agattctgtt ggttgtgtag
     6541 gctctgattc tgttaccatt gttgacccac ctgcaataac ttattctgca tcgcatacag
     6601 atgcaacctg tgccaatcta tgcaatggta tttcaaccat cactgcaacc ggcaatggca
     6661 ctctgactta tttatggaat actgtgcctc cacaaacaga ttctacaaac actaatttgt
     6721 gctttggcta cactacctat ataattacag acactaacaa ctgttctgtc accgattctg
     6781 ttttgatttt tgaaccttca ccaatattta tcgggaataa ttttttagga ataacctgta
     6841 atggaaattg tgatggtttt gttcgtgctc ttccatcagg tggaactccc ggatatactt
     6901 atttatggtc gaatggtgta actttcgatt ctatttttaa tacctgtgca ggcacttaca
     6961 cagttaccgt tacagatgcc aatctatgta cagctgttga ctcttttact tttgttgaac
     7021 ctgatccaat agttatcaat ttt
//
LOCUS       DAOOOL010000121         6959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1111_length_6959_cov_42.362978, whole genome shotgun sequence.
ACCESSION   DAOOOL010000121 DAOOOL010000000
VERSION     DAOOOL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_6959_cov_42.362978"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKU82_09440"
     CDS             <1..479
                     /locus_tag="PKU82_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOZ83137.1"
                     /translation="YAVRLILHEILPDKHFDIFYNEFGKPFLASPEGFISISHTHGFV
                     GVLFHRFNNCGFDMELMDRPVASISHKFVRSDEESFLKDNPLQKTLMLWSAKEVIYKI
                     YGKKSLDFKDNLRILPFEYQHQGEIKGQLILNNDICNYDIQYSFQDQLLTTFAIEK"
     gene            482..1234
                     /locus_tag="PKU82_09445"
     CDS             482..1234
                     /locus_tag="PKU82_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOZ83138.1"
                     /translation="MKQQILQQFHNHKKQLAVLIDPDKVNEDQLKKLCAYATEHQVDY
                     FFVGGSILTHGSISETIARLKKFASLPVILFPGNASQVDPQADAILYLSLISGRNADL
                     LIGQHVLSAMSVKLSGLEAIPTGYILIDSGNVTTVQYVSNTKPIPHDHSELAVSTAVA
                     GQLLGMQVIYLEAGSGARRTVSGQMIELVKKNTGLPLIVGGGIRTAEKAIEIYNSGAD
                     IIVVGNAIEDNPELMIEIAKAKTTINQTLNAL"
     gene            1238..1837
                     /gene="pnuC"
                     /locus_tag="PKU82_09450"
     CDS             1238..1837
                     /gene="pnuC"
                     /locus_tag="PKU82_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOZ83139.1"
                     /translation="MDLQTIMFLNIPLIEWIGFLFGVVGIYLGIKEKIAYFPISLINV
                     IVSLYIFYHQSLYSDVVQQGVYILLLSYGWYRWIKGSQNTEMPVTNASKKLIFILLGG
                     IILLTVGMGGWFSHYTDAHLPYWDALATSMCFAAQYLMAEKKIENWLVWIDANILYIG
                     IYLYKGLPLYAILFSIYLIMAIVGYFKWQNHLIRHEENS"
     gene            1821..2354
                     /locus_tag="PKU82_09455"
     CDS             1821..2354
                     /locus_tag="PKU82_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83140.1"
                     /translation="MRKIHKIAIVGPESTGKSLLSEQLSNHYNTAWVPEVARDYLHKL
                     NRPYTFDDLSVIAKLQLKEEERLSKTAKHFLFCDTNLIVIKVWSDFKYGRTEKWIVDA
                     IQKSDYSLHLLTDIDLPWEDDPLREHPHFRSQLFELYLQELNSFGLEYQTVRGIGEDR
                     IKNALNHIEKLEPNLIR"
     gene            2411..3709
                     /locus_tag="PKU82_09460"
     CDS             2411..3709
                     /locus_tag="PKU82_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOZ83141.1"
                     /translation="MMKKLLTLLFAVVILPVVALSQSPALVGYWHNWDITAAPYIMLD
                     SVDSRYNIIDIAFAVPQAGTDYQMEFIPEQVTPAAFATQVQNLQNQGRKVIISMGGAT
                     APVSLDNAVERDSFVASMTSIINFYGFDGIDIDFEGSSISVSGGTIANPIDAKIINLI
                     DAVKQIMSNYYATHGKKMILTMAPETAFVQGGMSAFGSIWGAYLPVIDALRDSLEILH
                     VQLYNSGTMFGIDGNIYTQGTADFIVAMTEAVIQGFNTSGGLFAGIPANKVAVGLPAC
                     TNAAGGGYTDTATVKSALDYLLGKGPKPGTYTLVQSGGYPTLRGMMTWSINWDAVNNC
                     STSSYQYAENFQKIFGTSTSLADVNQQRFSVFPNPASDKIYISTKSEGSKRFFIYDML
                     GKMIMSDNYSSTKEEIDISSLSNGVYYVKLENRIFKFVKY"
     gene            complement(3739..4575)
                     /locus_tag="PKU82_09465"
     CDS             complement(3739..4575)
                     /locus_tag="PKU82_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83142.1"
                     /translation="MKKLFTLIFFAIAGLVVADILNEQGQAVATGSPGEFDCTSCHTG
                     NNVNSGTGSISISSPNMPNWQYVPGQVYQIDVTVAQTGAPLFGFGFEALRTSNNTNGG
                     TFQITNTSSTFLKTGFINGSNRTNVVHKKNGGLSNDSHTFSFNWTAPATNIGNIIFYS
                     AGNAANNQGDTLGDFIYKTQQLITPSITGINEIQSSAISVYPNPATDFINVALPESQT
                     ETSYSILDLSGKIVDQGKMVIPQGKSFSVNLSQSLKEGIYFIEVSNGVDSYKHKFVKI
                     KN"
     gene            complement(4964..6580)
                     /gene="pckA"
                     /locus_tag="PKU82_09470"
     CDS             complement(4964..6580)
                     /gene="pckA"
                     /locus_tag="PKU82_09470"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOZ83143.1"
                     /translation="MNEYGLRNPNFSVAELGLKNVAAAYWNLTVAELVEEVVIRGEGE
                     LTDFGALAVDTGEFTGRSPKDKFIVADKETENSVWWGDINIKFDADKFDALYNRVTAY
                     MSGKEIYVRDCYACADPRYRLNIRVITETPYQSIFSNNLFLRPTPEEITTMEKDWVIM
                     AASGFMADPKIDGTRQHNFAIVNFTKKIILIGGTGYTGEIKKGIFSVLNYILPHHKNV
                     LSMHCSANIGKNNDTAIFFGLSGTGKTTLSADPNRGLIGDDEHGWSDNTVFNFEGGCY
                     AKCVNLSKEKEPQIYQAIRFGALLENTEFFEGTTRVDFDNISKTENTRVAYPIHFIDN
                     AVEPSVAGIPKNIFFLTCDAFGVLPPISRLTTGQAMYHFISGYTAKVAGTEVGITEPT
                     TTFSACFGKAFLPLHPTKYAELLGKKLSENKDIRVWLVNTGWSGGSYGVGSRIKLAYT
                     RAIITAALNGELDNVKFETLPVFGLQYPVSCPNVPAEILNPKATWADKTSFDNKSADL
                     AAKFVKNFEQYASHANKEIMDAAPKVAVVA"
     gene            6758..>6959
                     /locus_tag="PKU82_09475"
     CDS             6758..>6959
                     /locus_tag="PKU82_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOZ83144.1"
                     /translation="MFKKILIANRGEIALRVMRTASDMGIKTVAVYSDADRMAPHVLY
                     ADEAVHIGASPSAQSYLVVEKII"
BASE COUNT         2191 a         1210 c         1331 g         2227 t
ORIGIN      
        1 ggtatgcagt tcgattgatt ctgcatgaga tattgcctga taaacacttc gatatttttt
       61 ataatgaatt cggcaaacca tttttagcct cacctgaagg attcatttct atttcacata
      121 cacatggatt tgtgggagta ttgtttcacc gtttcaacaa ttgcggtttt gacatggaat
      181 taatggacag gcctgttgct tctatttctc ataagtttgt ccgaagcgat gaagaatctt
      241 ttttaaaaga caatcctttg caaaaaacgc tgatgctttg gagcgctaaa gaagttattt
      301 ataaaattta tggtaaaaaa tcgctcgatt ttaaagacaa cctgcgtatt ttaccctttg
      361 aatatcaaca tcagggcgaa attaaaggtc agttaatttt aaataatgat atttgcaact
      421 atgatattca atattcgttt caagaccaat tgctaactac tttcgccatc gaaaaataaa
      481 tatgaagcaa caaatacttc agcaattcca taaccataag aagcagcttg ctgttttgat
      541 tgaccccgat aaggtcaatg aagatcagtt gaaaaaactt tgtgcttatg caacagagca
      601 tcaggttgac tatttctttg ttggaggaag tattttaacg catggaagca tttctgaaac
      661 tattgccaga ttaaaaaaat ttgcatcctt gcccgtcatt ctttttcctg gtaacgcttc
      721 gcaagttgat ccgcaggctg atgcaatttt atatctttct ttaatatcag gtcgtaatgc
      781 tgatttgctg attggccagc atgtgcttag tgctatgtct gtaaaactat cagggcttga
      841 ggctatacct acaggataca ttcttattga cagtggtaat gtaacaacag tgcagtatgt
      901 aagcaataca aaacccattc cccacgatca tagcgaactt gcagtttcta cagctgttgc
      961 cggacaactt ttaggcatgc aggtaattta tcttgaagcc ggaagtggag ccagaagaac
     1021 cgtatccgga cagatgattg aattggttaa aaaaaataca ggtctgcctt tgatagttgg
     1081 tggtggtatt cgtactgccg aaaaagcaat tgagatttat aattcaggtg ccgatattat
     1141 tgtggtagga aatgccattg aagataatcc cgaacttatg attgaaattg caaaagcaaa
     1201 aacaactata aaccaaacct taaacgcatt gtagcctgtg gacttacaaa ctattatgtt
     1261 tttaaatatt cctttgattg aatggatagg ctttttgttt ggagtggttg gaatttattt
     1321 aggaataaaa gaaaagattg catactttcc gattagttta attaatgtaa ttgtatcact
     1381 ttatattttt tatcatcagt cattatacag tgatgttgtt caacaaggtg tttacattct
     1441 attgctttcc tatggttggt atcgctggat taaaggcagt caaaacacag aaatgcctgt
     1501 taccaacgct tcaaaaaaac tcattttcat tcttcttggt ggaatcattt tacttactgt
     1561 tggcatgggt ggttggttca gtcattatac tgatgcacat ttaccgtatt gggatgcatt
     1621 ggcaacaagc atgtgttttg cagcacaata tttaatggcc gaaaagaaaa ttgagaactg
     1681 gcttgtttgg atagatgcga atattttata tataggaata tatctttata aaggtctgcc
     1741 gctctatgca attttgtttt ctatttatct tatcatggct attgtaggat attttaaatg
     1801 gcagaaccat ctcatcagac atgaggaaaa ttcataagat tgccatcgtt gggcctgagt
     1861 caacaggaaa gagtttatta agtgaacagt tatccaatca ttataatact gcctgggtgc
     1921 ctgaagttgc acgtgactat ttacataaat taaatcgtcc atacaccttt gacgatttgt
     1981 cggtaatagc aaaattgcag ttaaaggaag aagagagatt gtcaaaaact gcaaaacact
     2041 ttttgttttg cgataccaat ttaatcgtga taaaagtttg gagcgacttt aaatatggac
     2101 gaacagaaaa gtggattgtt gatgcaattc agaagagtga ctattcatta catttgctta
     2161 cagatattga tttaccttgg gaagatgatc ctttaagaga acatccgcat ttcagaagtc
     2221 aactgtttga attatattta caagaattga atagctttgg attagagtat caaactgtac
     2281 gaggaatagg agaggacagg ataaaaaatg cattgaatca tattgaaaaa cttgaaccga
     2341 atttaataag gtgaatagaa atctgtattg attattttcg catctgatag aatttcttta
     2401 attaaaaata atgatgaaaa aactacttac acttttattt gcagtcgtta tcttaccggt
     2461 agtggcattg tcgcagtcgc ctgcattagt tggttactgg cataattggg atattacagc
     2521 agcaccatac attatgcttg atagtgttga ttcacgttat aatattattg acattgcatt
     2581 tgcagtgcct caggcaggca ccgactatca gatggaattt attcccgagc aggttactcc
     2641 tgcagcattt gctacgcagg ttcagaattt gcagaatcag ggacgcaagg taattatcag
     2701 tatgggaggt gccacagcac cggtttcatt agataatgct gttgagcgtg attcttttgt
     2761 tgcaagcatg acgagcataa ttaacttcta tggttttgat ggaatagata ttgattttga
     2821 aggcagttct atttctgtaa gtggcggaac aattgcaaac cctattgatg caaaaatcat
     2881 caacctgatt gatgcagtaa aacaaatcat gagcaactat tatgctactc atggcaagaa
     2941 aatgatactt accatggcac ctgagactgc atttgtgcaa ggaggcatgt cggccttcgg
     3001 tagtatttgg ggtgcctatt tacctgtcat tgatgctttg cgcgattcgt tagaaatatt
     3061 acatgttcag ctttacaaca gcggaactat gtttggcatt gatggtaaca tttacacaca
     3121 aggcactgcc gactttattg ttgcaatgac agaggcagtc attcaagggt ttaatacatc
     3181 tggtggtttg tttgcaggca taccggctaa taaagtggca gtgggattgc cggcatgtac
     3241 aaatgcagca ggaggtggtt atactgatac tgcaactgtt aaatcggcac tagattattt
     3301 acttggcaaa gggccaaaac ctggaacata cacattggtt cagtctggtg gctatccaac
     3361 tttacgtggt atgatgacat ggtcaatcaa ttgggatgct gtaaataatt gctcaacttc
     3421 gtcttatcaa tatgcagaaa acttccagaa aatatttgga acaagcactt cattagctga
     3481 tgtaaaccaa cagcgttttt ctgtattccc aaatcctgcg tctgataaaa tttatatctc
     3541 aacgaagtca gaaggtagca aacgattttt tatttatgac atgcttggta aaatgattat
     3601 gtctgataac tattcgtcaa caaaagaaga gatagatatt tcttccttat caaatggagt
     3661 atattatgtt aaattagaga atcggatttt taaatttgta aagtactaag cctcttttta
     3721 accctttgtt agggctaatt agttcttgat cttaacaaac ttatgcttat agctgtcaac
     3781 gccattactg acttcaataa aataaattcc ttctttcaat gattgggaaa gattaacaga
     3841 aaagcttttc ccttgtggaa taaccatctt tccttggtca acaattttac ctgacaagtc
     3901 aagaattgaa taggatgttt cagtttgact ttcgggcaat gctacattga taaaatctgt
     3961 tgccggatta ggataaacag aaatggcaga tgattgaatt tcatttattc ctgtaataga
     4021 aggcgtaata agttgttgtg ttttgtaaat aaaatcacct aatgtatctc cttggttgtt
     4081 tgcagcattt ccagctgaat agaaaataat atttccaatg ttggttgcag gagctgtcca
     4141 gttaaatgaa aatgtatgcg aatcatttga gagtccgcca tttttcttat ggacaacatt
     4201 ggttctgttt gaaccattta taaaacccgt cttaagaaat gtgctggaag tgttggtaat
     4261 ctgaaaagta cctccgtttg tattgttact tgtccggagt gcttcaaacc caaatccaaa
     4321 cagcggagca ccagtttgtg caactgtaac atcaatttga tacacctggc ctggaacata
     4381 ttgccagtta ggcatatttg gtgatgaaat tgaaatggaa ccggtaccgc tatttacatt
     4441 attgcctgta tgacatgatg tacaatcaaa ttcaccggga gaacctgtgg caacagcctg
     4501 tccttgttca tttaaaatat ctgcaacaac aagacctgca attgcaaaga aaattagtgt
     4561 aaagagtttc ttcataacct gtatttttat tttaacaggt caaatatgat gacaatttgt
     4621 aaaaatcaaa aaagcaatag ctctaaaagg aataatttcc ttccgaaaag gggataaact
     4681 tatctgtact gtttaaaaac tacggcaaca aattacttgc tgacatgaaa atggaaatgg
     4741 tggcggttgc tttttgaacg caccttaata cagaaaaaga aaacgatggc tgtaatgttg
     4801 agtaatgtaa atagtaataa catgcctcta aagtaatatc aacatcgaag agaatatata
     4861 ctatttggta aatggaaagt ttttttaggc aaaaatcaat tcagcataag tcaaaaaaaa
     4921 ctcccccgcg attttcgcgg gggagaaaaa aaattaaaaa aaattaagct actacagcca
     4981 ccttaggtgc tgcatccata atttctttat tagcatgtga agcatactgc tcaaaattct
     5041 tcacaaactt agcagctaaa tcagctgatt tattgtcaaa tgaagtttta tctgcccatg
     5101 tagctttggg attcagaatt tcagcaggca cattaggaca tgaaacggga tattgtaatc
     5161 caaacaccgg taaggtttcg aacttaacat tatctaattc accatttaat gcagcagtaa
     5221 tgatggcgcg tgtatatgca agtttaattc ttgaaccaac accataagaa ccaccgctcc
     5281 atcccgtatt cactaaccaa acacgaatgt ctttattttc acttagtttc ttaccaagta
     5341 attcagcata tttcgttggg tgcaggggta gaaatgcttt accaaaacaa gcagagaatg
     5401 ttgttgtagg ttcagtaatg ccaacttctg ttcctgcaac ttttgcagta tatccactga
     5461 tgaagtgata cattgcctgg cctgttgtaa gtcttgaaat aggaggcaat acaccaaagg
     5521 catcgcaagt taaaaagaag atgttctttg gaataccggc cactgaaggt tcaacagcat
     5581 tatctataaa atgtattggg taggctacac gagtgttttc tgtttttgaa atattgtcaa
     5641 aatcaacacg ggttgtacct tcaaagaatt ctgtattttc taagagtgca ccaaagcgta
     5701 ttgcctgata aatctgtggt tctttttctt tgctcaggtt tacacattta gcataacatc
     5761 caccttcaaa attgaataca gtattgtcgc tccaaccatg ttcatcatca ccaattaaac
     5821 ctctgttagg gtcggcactc aatgttgttt ttcctgtacc ggaaagtccg aagaagatag
     5881 cagtatcatt gtttttacca atgtttgcag agcagtgcat actcaataca ttcttatgat
     5941 gtggcagaat gtaattcagt acagagaaaa ttcctttttt tatctctcct gtataacctg
     6001 taccgccaat aagaataatc tttttggtaa aattaacgat ggcaaaatta tgctgacgcg
     6061 ttccatcaat ttttggatcg gccatgaaac ctgatgctgc catgattacc caatcttttt
     6121 ccatagtagt aatctcttcc ggtgtaggtc ttaaaaataa gttgttagag aaaatacttt
     6181 gatatggcgt ttcggtaata acacgaatgt ttaaacggta gcgtgggtct gcacaagcat
     6241 agcagtcacg tacataaatt tctttgccgg acatatatgc tgtaactctg ttataaagag
     6301 catcaaattt atcggcatca aacttaatat tgatatcgcc ccaccaaaca gaattttcag
     6361 tttccttgtc ggcaacaata aacttatctt ttggagaacg tcctgtaaac tcaccggtat
     6421 ctactgcaag ggcaccaaag tctgtaagtt caccttcacc tctgattaca acttcttcaa
     6481 caagttctgc aactgtcaga ttccaatagg ctgctgctac gttctttaaa ccaagttcag
     6541 caacagaaaa gtttgggttt cttaatccgt attcattcat aatatttcat cgtttttatg
     6601 aggtgcaaag taacaaaaaa taaagaaatt caagatgact tatagttact tttctgtaca
     6661 gaacttatta acctgtattt tggaaaaatt tagcatttgt aaaaggtttg attcacagtt
     6721 cgttttacct ttgcccaata atgtaaaaag cgacttcatg tttaaaaaaa tattgattgc
     6781 caacagaggt gaaattgcat tgcgggttat gcgtactgca agtgatatgg gaattaaaac
     6841 ggttgctgtt tatagcgatg ccgaccgcat ggcaccacac gttctatatg cagacgaagc
     6901 tgtgcacata ggtgcctcac catcagcaca atcttactta gtagttgaaa agattattc
//
LOCUS       DAOOOL010000122         6868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1131_length_6868_cov_68.716865, whole genome shotgun sequence.
ACCESSION   DAOOOL010000122 DAOOOL010000000
VERSION     DAOOOL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1131_length_6868_cov_68.716865"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKU82_09480"
     CDS             complement(<1..388)
                     /locus_tag="PKU82_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ83145.1"
                     /translation="MKSSSPSRLQHVLLIDDNEIDNFINERIITSSGFSEVCTVKTSA
                     DEAIEYLNIISKDSKVPLPQVIFLDLNMPVKDGFAFLDEFNALAENIRQSCRIIVLSS
                     SISPDDINRASANPYVIRYLNKPLAEK"
     gene            complement(413..485)
                     /locus_tag="PKU82_09485"
     tRNA            complement(413..485)
                     /locus_tag="PKU82_09485"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(450..452),aa:Phe,seq:gaa)
     gene            695..1213
                     /locus_tag="PKU82_09490"
     CDS             695..1213
                     /locus_tag="PKU82_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83146.1"
                     /translation="MLIQNKKILIATLAVLFFIGISFHGYCITSEPEHSHQHHKAEIG
                     VGQSAVYFTKDKTLNYGLHLHLIRNIKHSGFGVGIEYERIFDRHGHNMLGIVGSYSPA
                     HQVELSITPGILFEDSEPGHADASIHIEAGYHFDIKNIHLGPIVECALDRHDVHFSLG
                     VHVGIGLGKIRE"
     gene            1596..2033
                     /locus_tag="PKU82_09495"
     CDS             1596..2033
                     /locus_tag="PKU82_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOZ83147.1"
                     /translation="MKERFNLSKADPKAFDAMLNLEKYLAESGLDKILFELIKTRASQ
                     INGCAYCINMHVRDAMKIGETSQRLFLLDAWRETTLFTEKERAVLALTEAMTLIANNL
                     VSDTVFDDAKKHLTDKEMAAVMMTIVTINGWNRIAITSRTALD"
     gene            2095..3531
                     /locus_tag="PKU82_09500"
     CDS             2095..3531
                     /locus_tag="PKU82_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ83148.1"
                     /translation="MSQEAIIDQLNKLNLISKQLDLPESQRTELLHQTADYANNFIST
                     LHTIKGYQEKTVGDLSINNKPAALHDILNLYQKEVAESGINAASPKHLGYIPGGGVYT
                     AALADFIAAVTNPFAGAYFAAPGAATIENEVVNWLKNVFSFPERAVGCLSSGGSVSTL
                     IAFTAARDKFKIKNEVVPKSVVYLSEQVHHSTQKALRIIGLEDVIVRYIPLDDCHKIK
                     PDALIALIEKDKAEGLHPFLIVATAGTTDTGTVDPLNDIADIAAQQKLWFHVDAAYGG
                     FFILTSKKNLFKGIERADSIIVDPHKGLFLPYGVGAVLVKDGNAVLHSHYYTANYMQD
                     GQNEELLKSPANLSPELTKHFRGLRVWLPLKYHGIEPFIACLEEKLLLVQYFRMKLAE
                     QGFCLGPEPDLSVSYFWYPFATDADEKNKLLMEEMHKDGAVFLSSSIISNRFVIRMAI
                     LAFRTKKETVDEAVEMIDRCLKKVMLNS"
     gene            3566..4207
                     /locus_tag="PKU82_09505"
     CDS             3566..4207
                     /locus_tag="PKU82_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83149.1"
                     /translation="MTFHKSVFLLIFLFATNFCSGQIDIYANKYPLFGTTRCGICFGN
                     WCNYNGYRFNNTDTASSPFNGINIFMYSDGNSGNGVQIGVIRAEYYQFNGVSFASISQ
                     YGSKLNGVGISAFGVVPDTLNGLGVGLFLSSDRKHEERILNGVGLSIFGADAGKMNGF
                     YIGGYCSSDRQKGLSIALFNHTKELHGLQLGVINYAGNNRKLFRWMPFFNVHL"
     gene            4617..5003
                     /locus_tag="PKU82_09510"
     CDS             4617..5003
                     /locus_tag="PKU82_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOZ83150.1"
                     /translation="MNTSIENELSYQIIGIAVEMHRVLGPGLLESAYESALTYDLIQA
                     GFDVKVQVPMPLIYKNVKQEVGYRLDLIINDKVIVEIKSVETLAPVHFAQTLTYLKLS
                     GKKLALLINFNTKYLKEGIHRIVNNL"
     gene            5871..6578
                     /locus_tag="PKU82_09515"
     CDS             5871..6578
                     /locus_tag="PKU82_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83151.1"
                     /translation="MKNTSLHHINSLEPAETSSITKRKLNLKSLSVFLLLFSLATLIT
                     SCKKDDDSNPLESTQYYNLQDGLRKLWNDHMKYTYATVDAYYNNPSGLSSSLNRLLQN
                     QKDIGAAIVPYYGQAAGDTLSSLLTTHINLAVPVLEAAKNNDQPALNLALANWKTNAN
                     DIANFLSTANPKNWPPHDMQHMMEMHIDQTTTYAVDLLNKDYNNAITHYDEANQHMLD
                     MADDLAKGIALQFPEKF"
     gene            6603..>6868
                     /locus_tag="PKU82_09520"
     CDS             6603..>6868
                     /locus_tag="PKU82_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOZ83152.1"
                     /translation="MENKIISQTFNLLKYTFVVVPIVAGADKFTNLLTNWPQYINPTI
                     VGMLPLTPAAFMMLVGVIEIVAGIVVLKKPIIGGQIVAAWLTLIA"
BASE COUNT         2325 a         1242 c         1207 g         2094 t
ORIGIN      
        1 atttttctgc taatggcttg ttcagatatc ggataacata aggattggca gaggcgcggt
       61 taatatcatc cggactgatg gatgatgaca acacaatgat gcggcatgac tgacggatat
      121 tttctgcaag agcattaaac tcatcaagaa aagcaaaacc gtcttttacg ggcatgttca
      181 ggtcaagaaa aattacctgt ggcaatggta ccttactgtc tttggatatg atgttcagat
      241 attcaatggc ttcatcggca gaggtcttta cggtacatac ctcgctgaac cctgaagagg
      301 tgataatacg ctcgttgatg aaattatcta tttcattatc atcaatcaac agaacgtgtt
      361 gcaatcgaga aggagaagat gatttcattg aatcttttcc tgaaaatgtt agtggtgaca
      421 ggtggaatcg aaccaccgac acaaggattt tcagtccttt gctctaccaa ctgagctatg
      481 tcaccgttaa tgagtgcgca aaagtaaaaa aataatgaat agaaaaaact ttttgttggg
      541 tttattgccc ctgtctcaga ccatttttca aaaacaggac acttattttc tttagctttg
      601 aacaaactat aattttattt cagacaaggt caaaagcgct cagggcttta atgaaaacca
      661 ttcttctcaa aaatcatttt tattacacat taaaatgcta attcaaaaca aaaaaatcct
      721 tattgccacc cttgcagttt tattttttat tggaatttct tttcacggat attgcatcac
      781 ctctgagcca gaacacagcc atcagcatca taaagctgaa ataggtgttg gccaatcggc
      841 agtttatttt acaaaggaca aaacattaaa ctatggtctg cacctccacc tgatacgcaa
      901 cattaaacat tccggttttg gtgtaggtat agagtacgaa agaatttttg accgccatgg
      961 acataacatg ctgggaattg tcggcagcta tagtccggca catcaggttg aactaagtat
     1021 tacaccgggc attttatttg aagactccga acccggacat gccgatgcat ccatacacat
     1081 agaagcaggc tatcatttcg acataaaaaa tattcacctt gggccaatag ttgaatgtgc
     1141 actcgacaga cacgatgtac actttagcct tggtgtgcat gtaggtattg ggttagggaa
     1201 aataagggag tgatggtttg gaggaaattt tatctgttgt tcttattaat agtttttata
     1261 tttacataaa aaactattaa tcctcttcgc actctttgcg tgaaagaaaa ctacacttgc
     1321 tacaaatacg cctttgcgct ctttgcgtga aagaaaaagt ctcttgctaa gactcaaaga
     1381 aaaaaaatac gcagaggccg ctaagaaaat tcattctttg cgaactttgc gtgaaatact
     1441 ttttcagaat agcgggtatg gctccatgaa agttgatata agtgatttta aacagggtat
     1501 ttattctatt tatgttagag acaataatga aaacaatttt gtaaaaaaat ttgttaagct
     1561 ttaattatat ttttacagtt aaaataaata ttaaaatgaa agaacgattc aacttatcaa
     1621 aagccgaccc taaagcattt gatgctatgc taaatcttga aaaatatctt gcagaaagcg
     1681 gattagataa aatacttttt gaactgataa aaacccgcgc ctcacaaatc aacggttgtg
     1741 cctattgtat taacatgcat gttcgcgatg ccatgaaaat tggtgaaaca tcacaacgat
     1801 tatttttatt ggacgcctgg cgagagacta ctttgtttac agaaaaagag cgtgcagttt
     1861 tagctcttac agaagccatg acattaattg caaacaatct cgttagcgat actgtttttg
     1921 atgatgccaa aaaacattta accgataaag aaatggcggc agtaatgatg accattgtta
     1981 caattaacgg atggaacaga attgcaatta catcaagaac tgcattagac taatttgcat
     2041 tctttaaacc aacaaacaca aataataaat tgattacttt actctatttt taagatgagt
     2101 caggaagcta tcattgacca attaaacaaa cttaatttaa tcagcaagca attggattta
     2161 cccgaatccc aaagaactga attgttacat caaactgctg attatgccaa caactttatt
     2221 tcaacactac ataccataaa aggttatcag gaaaaaaccg ttggtgattt atctatcaat
     2281 aataagcctg ctgctttaca tgatatactg aatctatatc agaaagaagt tgcagagagt
     2341 ggaattaatg cagcctcgcc aaaacattta ggctacattc ccggaggagg agtttacacc
     2401 gcagccttag ctgattttat tgctgctgtc accaatcctt ttgccggtgc atattttgct
     2461 gctcccggtg cagctactat agaaaacgaa gttgttaatt ggttgaaaaa cgttttttct
     2521 tttcctgaaa gggcagttgg gtgtttatca tcaggtggct ctgtttctac tttaattgcg
     2581 tttactgctg caagagataa atttaaaatc aaaaacgaag ttgttccaaa aagtgtagtt
     2641 tacctgagtg agcaggttca tcattccaca caaaaagctt tacggataat tggacttgaa
     2701 gatgttattg tacgttacat ccctttggat gattgtcata aaattaaacc tgacgcatta
     2761 attgcattaa tagaaaaaga taaagcagaa ggactccatc cctttttaat tgttgccact
     2821 gcaggaacaa cagataccgg tactgttgac ccattaaatg atattgccga tatagctgct
     2881 cagcaaaaac tttggtttca tgtagatgct gcctatggag gattttttat tctcacatca
     2941 aagaaaaatt tatttaaagg aatagaacgt gccgactcca ttattgttga cccgcataaa
     3001 ggtttgtttc taccctatgg tgttggtgca gttttagtga aagatggcaa tgcagtttta
     3061 cactcacatt attacaccgc caactacatg caggacggac aaaatgaaga gttgcttaaa
     3121 agtccggcaa acctatcacc ggagctcacc aaacattttc gcggtcttag agtgtggctg
     3181 ccattaaaat atcatggcat agaacctttt attgcttgtc ttgaagagaa acttttattg
     3241 gtacaatatt tcagaatgaa attggcggag cagggttttt gtctcggccc tgaacctgat
     3301 ttatcggtaa gttacttttg gtatcctttt gctactgatg ctgatgaaaa gaataaactg
     3361 ctgatggaag aaatgcataa agatggagct gtattcttaa gttcgtcaat cattagtaat
     3421 cggtttgtaa tacgaatggc cattttagcg ttcagaacaa aaaaagaaac tgttgacgaa
     3481 gctgttgaaa tgattgaccg gtgtttgaaa aaagttatgt tgaatagtta aatctacttt
     3541 attttaaaat ttaaaataat cctaaatgac atttcacaaa tcagttttct tactaatatt
     3601 tctatttgcc acaaattttt gtagcgggca aattgatata tatgcgaata agtatccctt
     3661 gtttggaaca acacgttgtg gaatctgttt tggtaactgg tgtaattata atggttatcg
     3721 ttttaataat accgataccg caagtagccc ttttaatgga ataaatattt ttatgtattc
     3781 cgatggcaat tcgggaaacg gagtacaaat cggagtaata cgtgctgaat attatcaatt
     3841 taacggtgtt tcattcgctt caatttcaca gtatggaagt aagttaaatg gtgttggtat
     3901 ttctgcattt ggagtagtac ctgatacttt gaatggttta ggtgttgggc tatttctttc
     3961 ttctgacaga aaacatgagg aaaggatatt gaatggagtc gggctgtcta tttttggagc
     4021 agatgcaggt aaaatgaatg gattttatat agggggatat tgtagttctg acagacaaaa
     4081 aggattatca atagctttgt tcaatcacac aaaagaatta catggtttac aattgggtgt
     4141 tattaattat gctggtaata atcgaaaact atttagatgg atgccatttt tcaatgtaca
     4201 tctttaatta aaaccaccaa agaaaattat acggatacct cttgcgctct ttgcgtgaaa
     4261 tgaaaaacaa tacttactac taataccact ttgcgagctt tgcgtaaaaa gaaaaaggtt
     4321 tctcgctaag acgcaaagaa aaaaatcacg cagagaccgc taagaaatac atctcctttg
     4381 cgctctttgc gtgaaaagaa aactgcactt gctacaaata ccactttgcg agctttgcgt
     4441 gaaataaaaa aagttcctcg ccaagacgca aagaaaaaaa cgcgcagaga ccgccaagaa
     4501 atatattttc cttgcgagct ttgcgtgaaa aacaaacaca ctatattttg aataaacatt
     4561 tttatttaac aaaactattt tttgtatctt tcgatttgaa acaattataa accgaaatga
     4621 atacttcaat tgaaaatgaa ctctcatatc aaataattgg aattgcagta gaaatgcata
     4681 gagtcttagg cccaggctta ttagaatctg cttatgaatc tgctttaact tatgacttaa
     4741 ttcaggctgg ttttgatgta aaagtccagg ttccaatgcc attgatttat aaaaatgtaa
     4801 aacaagaggt tggatacaga ttagatttaa taatcaatga taaagttatt gttgagataa
     4861 aatctgtaga aacgctcgcc ccagttcatt ttgcacaaac gcttacatat ttaaaattgt
     4921 ctgggaaaaa attagctctc ttaatcaatt ttaacactaa gtatctaaaa gaaggcattc
     4981 atagaatcgt taataatttg taaatattag gatattatat ttaagcaaat tatttaatag
     5041 tttacattct atcatttctg ctttaacatt cacagactat ttaaaattat tatccctttg
     5101 cgagcttttc gtgaaagaaa aaaggtttct cgccaagacg caaagaaaaa aatcacgcag
     5161 agaccgctaa gaaatatatc tcccttgcga gctttgcgtg aaaaaaagat tgctcgctaa
     5221 gacacaaaga aaaaaacacg cagaggccgc taagaaaatt cactctctgc gctctttgcg
     5281 tgaaaagaaa actacacttg ctacaaatac ccctttgcga gctttgcgtg aaataaaagt
     5341 ctctcgccaa gacgcaaaga aaaaaatcac gcagagaccg ctaagagaaa aaacctttgt
     5401 gctctttgcg tgaaatgaaa aaagccacac atggcgcaag tcttatgact tgtgcccaca
     5461 tatcattcta ctaataaaca cattctggta gctatcagaa tatggctttt gcccaaccat
     5521 cgttaaaaaa agccctaaca tcttcgtcat ctatacatta agttttgcag ttgggaatta
     5581 tataggtaat aatcgaaaac tatttaggtg gatgccattt ttcaatgtac atctttaatt
     5641 aaactaacaa aagaaagcta aatgataacc catcgcgctc tttgcgtgaa aagaaaatta
     5701 cacttgctac aaatatcact ttgagagctt tgcgtgaaat gaaaaagcct ctcgccaaga
     5761 cgcaaagaaa aaacacataa agtccaactt taatacaatt ttagattttt tgtaaccttt
     5821 tgttttttcg tttactctaa tagagtataa acattaaaaa aataataaaa atgaaaaaca
     5881 catcattaca tcacatcaat tctcttgaac ctgctgaaac ttcaagtata accaaaagaa
     5941 agttgaattt gaaatcgctc tctgtatttc tactcttatt ttctcttgcc actttaatta
     6001 catcttgcaa gaaagatgac gactctaatc cattagaaag tacacaatat tacaacttgc
     6061 aagatggctt gcgcaaactc tggaacgacc acatgaaata cacttatgca actgttgatg
     6121 cctactacaa caatccttcc ggattatctt cttctttaaa tcgtttatta caaaatcaaa
     6181 aagatattgg cgctgcaata gttccttact atggtcaggc tgccggtgac acactttctt
     6241 ctttgctcac aacacatata aacttagctg tgcctgtact cgaagccgca aaaaataacg
     6301 atcagcccgc ccttaacctt gcattagcaa actggaaaac caatgctaac gatattgcaa
     6361 actttctttc aactgctaat cctaaaaact ggccaccgca cgatatgcaa cacatgatgg
     6421 aaatgcacat tgaccaaact acaacatacg ctgttgattt attgaataaa gattacaaca
     6481 atgcaatcac tcactatgat gaggccaacc aacacatgct cgacatggct gacgaccttg
     6541 ccaaaggaat agcacttcaa tttccggaga aattttaaat taacttcaaa ttcaaaaaaa
     6601 ttatggaaaa taaaatcata tcccaaacat tcaacttact taaatacact tttgtagttg
     6661 ttcctatcgt tgccggtgca gataaattta caaaccttct taccaattgg ccacaatata
     6721 ttaatccaac aatagtcggt atgcttccgc taactcctgc agcatttatg atgcttgttg
     6781 gtgtaattga aatcgtagca ggaattgttg tcttaaaaaa acctataatt ggtggtcaga
     6841 tagtagctgc atggcttaca ttaattgc
//
LOCUS       DAOOOL010000123         6693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1165_length_6693_cov_61.912775, whole genome shotgun sequence.
ACCESSION   DAOOOL010000123 DAOOOL010000000
VERSION     DAOOOL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_6693_cov_61.912775"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..2004)
                     /locus_tag="PKU82_09525"
     CDS             complement(244..2004)
                     /locus_tag="PKU82_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ83153.1"
                     /translation="MAETNSTSGFIAIPDAKAWDPAWSTENKVVYQVLQEPDNLHPTN
                     GKTLMRSEVMLYTQGVLIGTDFKTNSLKPNLLSKLPVYDANKSSYNFHLRNDVQWDNG
                     EHLTANDILFTLKVYKSALTQNDFFKPYLENMLDFIPDVNDSLAFSIKIAKPYLQDVA
                     IWGDIIIMQQSFHDPKNILAKYSLTTLATEDFRANAPADVKKWFEEFNSEATGFDLKK
                     LNGLGPYKVTAWEHGQFLTLEKKNNHWTDHSKDSSEHSSVAKIIFKISSDPNVNVLEF
                     KKQQFDASTTLATKSLLKLREDTLFNHNYNAQFTSTFNYSFAAFNMKPDGKKHKKLFD
                     NLQVRKAMAMLFPYEQINRALYKGEYKRQAGPVCSHKYNFNESLTPILQNYNEATKLL
                     ADAGWKDSDNDGVLDKMIDGIKEDFKFDMNIYAGIPDWNDLSLMLVAELKKAGIVMNI
                     VPLEVPVFLEKARTHDFDMLMSVWTTNVSPDDFTQLWHSSSWRENGDNYSGFGNSASD
                     ALIDSIKFTIDDSLRTALDKRFQKIVVDDMAYIFLFNSQRRIAVHKRFGNVNLYFEKP
                     GLLINTFRLLSPVNKTSATG"
     gene            2188..2276
                     /locus_tag="PKU82_09530"
     tRNA            2188..2276
                     /locus_tag="PKU82_09530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Ser,seq:gct)
     gene            2368..2443
                     /locus_tag="PKU82_09535"
     tRNA            2368..2443
                     /locus_tag="PKU82_09535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2404..2406,aa:Pro,seq:tgg)
     gene            2501..2574
                     /locus_tag="PKU82_09540"
     tRNA            2501..2574
                     /locus_tag="PKU82_09540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2535..2537,aa:Arg,seq:tct)
     gene            complement(2605..3639)
                     /locus_tag="PKU82_09545"
     CDS             complement(2605..3639)
                     /locus_tag="PKU82_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOZ83154.1"
                     /translation="MIKQSVFIFLVCFLIFSCKKDKPETIIQPLITSNNNGVVYITNE
                     GNFQFGNAAISLYDKESKNIIEDYYKQVNQVPLGDVCQSMTYFNNKFYIVVNNSSKVM
                     VVNAATFKIEATISGFNSPRYFIPVSNSKAYVSDLYDNRLTIVNLSTNTISGYIPAVA
                     GTEQMKMVYGKVFVSSLFTDKVYVINAMTDMLTDSITLAYSPNSICEDKNGILWVLCS
                     GDSLKQKPSALIQMNPLNNQVIKSFVFTGNHAPWRMCMNQQNDTLYFLDRHVWQMSIS
                     DTTLPSTPFINGNGKLFYTLSVDASNGEIYVSDAIDYLQRGSIYRYSASGNLIDQFKA
                     GIIPGGFCFR"
     gene            complement(3632..4645)
                     /locus_tag="PKU82_09550"
     CDS             complement(3632..4645)
                     /locus_tag="PKU82_09550"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83155.1"
                     /translation="MFDSLKTVLIALACLFSLTESNGQFAPPASQPGTTAIHKDSSII
                     VSWATQCSIVRGWQDISNHSLGVCTIGDSTSALGMADGLDVVSLGDGGMATLTFANPI
                     MNGSGWDFAVFENSFSETFLELAFVEVSSDGVNFFRFPSVSLTQDTVQVGSFGSIDAT
                     QIDNLAGKYSAFYGTPFDLDIMTGISGLDVNHITHVRVIDVVGSIDELYATYDSQFNK
                     INDPWPTPFPSSGFDLDAVGVIHQNTTSVNSIIENQFNLSYPNPFNKNNVIKLNLTKT
                     EDLSMTLYDISGKAIYQFLDGKINAGEHLFNLRNSLIGNGIYFLKLSSKEHTETFKLI
                     VND"
     gene            complement(4630..5613)
                     /locus_tag="PKU82_09555"
     CDS             complement(4630..5613)
                     /locus_tag="PKU82_09555"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOZ83156.1"
                     /translation="MKRVRLIIVTMSLFQIVSVKLNAQTVSDFENLTLPADSFFNGSS
                     QQGGTSFTSGNAIFPNFFDPSFQYWLSGWAYSNVADSSTAGFGNMYASASLTGYNGSG
                     IYALGQQNATITLSGNASGKVVNGFFVTNATYPYISMRDGDGFSKKFGGPSGNDPDYF
                     ILTIHNWYNGNLSSDSINFYLADFRSANNAQDYIINDWQWVDLTGLGNTDSLLFTLSS
                     SDVGSFGINTPLFYCIDNFTTADSPVGISEIKNNVVQVYPNPASSYISLTKNIDEIFI
                     TDVEGRVVMESLKLNAGDKISVSQLKDGLYFINAHYNNESTKFKFVKCSTH"
     gene            complement(5616..>6693)
                     /locus_tag="PKU82_09560"
     CDS             complement(5616..>6693)
                     /locus_tag="PKU82_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ83157.1"
                     /translation="LSKGQLAVRVAWFNQGIHYSDSLKNMNATSRAKSLLAEAEYKFH
                     PGKHREIHAGITNNYTTATADEYKKQAQLNSFALFIGETAHFFNNKIKPSVSFRKEFN
                     DNKEAPFTWYAGGEIALYKNISLKLNAASVYRNPTINDLYWQPGGNKNLKPESGKTGD
                     IGLCLNEINLAKNITAKTEITLFNRKMKNQIIWLPENGYWSAQNLDRTESKGIETKTI
                     IRYSNKKWFTALTVSTNYIEATNEKARFENDASVGKQLIYVPMYSGNGSIAIGYGKLI
                     VKYTVQYSGYRYTTTDNLDYLEPFWLHGFYAACSLSAQKTKFQLYLKINNLLDENYEA
                     IRNRSMPLRSYKAGIIIQINSKSK"
BASE COUNT         2027 a         1226 c         1220 g         2220 t
ORIGIN      
        1 agctaaggat attacagaaa aataaaataa aggcttgtca tagcctagac gttgtgcaag
       61 taatgttttc tggtgctcat aattagttga agtaatactt gttttttctt ctcgtatgag
      121 aatgtaatcc aatgcatcac cgggcaaata aacagatagc ataaacgatg aaaccaacag
      181 tacagaaaaa ataaaaaagt atttgaaaat gatatgccgc atcttcctta gtctgaattt
      241 tatttatcct gttgcagaag ttttgttaac cggtgaaagt aatcgaaaag tattgatgag
      301 taatccgggt ttttcaaaat aaagatttac atttccgaat cgtttgtgca ctgcaattct
      361 gcgttgtgaa ttaaaaagaa aaatatacgc catatcatca accactattt tctgaaaacg
      421 cttgtccaaa gcagtccgca aagaatcatc aatggtaaat tttattgaat caatcaatgc
      481 atctgaagca ctgttgccaa atcctgaata gttgtcacca ttttcgcgcc agcttgacga
      541 atgccatagc tgcgtaaaat catcaggaga aacatttgtt gtccacacac tcataagcat
      601 atcgaaatca tgtgttcgcg ctttctctaa aaacacaggt acttcaagag gaacaatatt
      661 catcactata cctgcttttt ttaattcagc aacaagcatt aaagaaagat cgttccaatc
      721 gggaatgccg gcatagatgt tcatgtcaaa tttaaaatct tcttttatgc catcaatcat
      781 tttgtcaagt acaccatcgt tgtcactatc tttccaaccg gcatcggcaa ggagtttagt
      841 ggcttcattg tagttttgta aaatgggtgt aagcgattca ttgaaattgt atttatgact
      901 gcacaccggt cctgcctgtc ttttgtattc gcctttatac aatgccctgt ttatttgttc
      961 atagggaaaa agcattgcca tagcttttct tacttgtaaa ttatcaaata actttttatg
     1021 cttctttcca tctggcttca tattaaaagc ggcaaatgaa tagttgaatg tacttgtaaa
     1081 ctgagcattg taattatggt tgaataaagt atcctcacgc aatttgagta atgattttgt
     1141 tgcaagtgtt gtgctggcat caaattgctg ctttttaaat tcaagaacat tcacatttgg
     1201 gtcgctgctg attttgaaaa ttatttttgc gacagaagaa tgttctgatg aatccttgga
     1261 atgatctgtc caatgattgt ttttcttctc caatgttaga aactgaccat gttcccatgc
     1321 agtaacttta tatggcccta acccatttag ttttttcagg tcaaaacctg ttgcttcgga
     1381 attaaattct tcaaaccatt ttttaacatc tgccggtgca ttggctcgaa aatcttcggt
     1441 agctaaagtt gtcagggagt attttgcaag aatatttttt ggatcgtgaa atgattgttg
     1501 cataataata atgtcacccc agatggcaac gtcctgcaga tagggttttg caatttttat
     1561 gctgaaggca agcgaatcat ttacatcagg aataaagtca agcatatttt ctaggtaagg
     1621 cttgaaaaaa tcattctgtg taagtgcact tttgtaaact ttcaaagtaa aaagaatatc
     1681 attggcagta aggtgttcac cattgtccca ttgaacatca tttcttaaat ggaagttata
     1741 tgaacttttg ttggcatcat aaacaggtag tttactaagc agattgggtt ttaaagaatt
     1801 tgttttaaaa tctgttccaa tcagtacacc ttgcgtataa agcataactt cactacgcat
     1861 cagggtttta ccatttgttg ggtgcagatt gtcgggctcc tgaagtacct gatacacgac
     1921 tttattttct gtactccaag ccggatccca tgcttttgca tctggaatag caatgaatcc
     1981 tgaagtgctg tttgtctcag ccacagtgta attctgcggc ttgtggtctg tgcaagagac
     2041 cattaaaatc aaaaatgcga ataaaatgtt gataccgaca actctcataa aggcaatatt
     2101 agcatataag aggcataaaa aaataaaatt aaagcatata gttttgtttg catatacttt
     2161 tttttacctt tgccgcccat ctgaaaaggt gaggtgcctg agaggccgaa aggaacagtt
     2221 tgctaaactg tcgtacgggg caacctgtac cgtgggttcg aatcccaccc tcaccgcaaa
     2281 agttttgcga aaatcaggtg taaggtgaag aaaatgtttc ttcgcatgaa agttgttctt
     2341 tgagtcgtaa ccgaatttaa aaattttcgg ggtgtagcgt agcccggttc atcgcgcctg
     2401 ctttgggagc aggaggtcgc aggttcgaat cctgccaccc cgacaaaaaa agagagtcta
     2461 aagcggttgc cgctttagac ttttatgttt acggctgttt ggtcccgtag ctcagctgga
     2521 tagagcaact gccttctaag cagtaggtca ttggttcgaa tccaatcggg atcacaactt
     2581 ttcttagtca tcccgaatca aattttaccg aaaacaaaat cctccgggga tgattcctgc
     2641 tttaaactga tctatcaaat ttcctgaagc agaataacga taaatacttc cacgttgcaa
     2701 atagtctatt gcatccgata cataaatttc accattcgaa gcatcaacac tcaaagtgta
     2761 aaataatttt ccgtttccat tgatgaatgg tgttgaaggt aatgtagtgt cgctgatgct
     2821 catttgccat acatggcgat caagaaaata aagagtgtca ttttgctgat tcatacacat
     2881 acgccagggg gcatggttgc cggtaaaaac aaaactttta ataacctgat tatttaacgg
     2941 attcatctgt atcaatgcag atggtttttg ttttaacgaa tcaccactgc acagcaccca
     3001 taatatgccg tttttatctt cacagataga attgggcgaa taagctaaag ttatgctatc
     3061 cgtcagcatg tctgtcatcg cattgatgac ataaacctta tcagtgaaga gtgatgaaac
     3121 aaaaactttt ccataaacca ttttcatttg ttctgtgcct gcaactgcag gaatataacc
     3181 tgatatggtg ttggtagaaa gatttacaat ggtcagtctg ttgtcgtata aatccgatac
     3241 ataagctttg ctgttgctga caggaataaa ataacgtggt gaattaaaac ctgaaatagt
     3301 tgcttcaatt ttaaaagttg cagcattaac taccatcact ttagaagaat tgttgactac
     3361 aatgtaaaac ttattattaa agtaagtcat actctggcaa acatcaccta aaggaacttg
     3421 atttacctgc ttgtaatagt cttcaataat attttttgat tctttatcat acaggctgat
     3481 tgctgcattt ccaaactgaa aatttccttc attggtaata tacaccactc cattattgtt
     3541 tgaagtaatt aaaggttgta ttattgtctc aggcttgtct tttttacagg aaaaaataag
     3601 aaagcaaaca agaaatataa atacagattg cttaatcatt aacaataagt ttaaaggttt
     3661 ctgtatgctc tttagatgat aattttaaaa agtaaattcc attaccaatt aaagagtttc
     3721 taagattgaa aagatgttca ccggcattga tttttccatc taaaaactga taaatagctt
     3781 ttcctgaaat gtcatataat gtcatactta ggtcttcagt cttagtaaga tttaatttaa
     3841 tcacattgtt tttattaaat ggattggggt aggagagatt gaactgattt tctataatcg
     3901 aatttactga tgttgtattt tgatgaatga ctcctactgc atcaaggtcg aatccggaac
     3961 ttggaaatgg agtaggccag gggtcattaa ttttattgaa ttgcgaatcg taagttgcat
     4021 acaattcatc aatacttccc accacatcaa ttactctaac atgtgtgata tgatttacgt
     4081 caagccctga aattcctgtc attatatcta agtcgaatgg tgttccataa aatgcactgt
     4141 acttgcctgc cagattatca atttgtgttg catcaatact tccgaagctt ccaacctgca
     4201 cagtatcttg cgttaaagac actgaaggaa aacgaaaaaa gtttacacca tcactactca
     4261 cttcaacaaa tgcaagttcc agaaatgttt cgctaaagct gttctcaaat actgcaaaat
     4321 cccatccact accattcatg attggatttg caaatgttaa tgtagccatg ccgccatcac
     4381 caaggctaac tacatcgagg ccatcagcca tacctaatgc agatgtgctg tcgcctattg
     4441 tgcaaacacc taaactgtga ttagaaatgt cctgccatcc tctgacaata ctacattgtg
     4501 ttgcccacga aacaataatg ctgctgtctt tatgaatagc tgttgtgccc ggctgactgg
     4561 caggtggtgc aaactggcca ttactttcag ttaatgaaaa caggcaagcc aatgcaatca
     4621 atacagtttt taatgagtcg aacattttac aaatttaaat tttgttgatt cgttgttgta
     4681 gtgtgcattg ataaaatata atccatcttt tagttgtgaa acagatattt tatctcctgc
     4741 atttagtttg agtgattcca taacaacccg accttcaaca tcagtgataa aaatttcatc
     4801 tatattttta gttaatgaga tataggaaga tgcaggattt ggatatactt gtactacatt
     4861 gtttttaatt tctgaaattc ctaccggtga atctgctgtg gtgaagttgt caatacaata
     4921 gaataaaggt gtattgatac caaaacttcc aacatcgctt gaagaaagtg taaaaagcaa
     4981 actgtctgta ttgccaagtc cagttaaatc tacccattgc caatcgttga tgatataatc
     5041 ctgcgcatta tttgcagaac gaaagtcagc aagataaaaa ttaatactat cactacttaa
     5101 atttccatta taccaattgt gtatggttag aataaaataa tcaggatcat ttcctgatgg
     5161 ccctccgaat ttttttgaaa aaccatctcc atctctcata ctgatatatg gataagtagc
     5221 attggtaaca aaaaatccat taactacttt ccctgaagca ttgcctgaaa gtgttatggt
     5281 tgcattttgc tgaccaaggg cgtaaatgcc tgagccgtta taccctgtca atgatgcgct
     5341 ggcatacatg tttccgaagc ctgcagtgct gctgtctgca acattactgt atgcccagcc
     5401 cgaaagccag tattgaaatg atgggtcgaa aaagttagga aaaattgcat ttcctgatgt
     5461 gaaacttgtt ccgccttgct gtgatgagcc attaaagaag ctatctgccg gtaaagtcag
     5521 gttttcgaag tcactaaccg tttgtgcatt cagttttaca gacactattt ggaatagtga
     5581 cattgttaca atgatgagtc taactctttt catatttatt ttgattttga atttatctga
     5641 ataataattc ctgctttgta tgagcgtaag ggcattgaac ggtttcgaat tgcttcatag
     5701 ttctcatcaa gcaggttgtt gatttttaag taaagttgaa attttgtttt ttgtgccgat
     5761 agactgcatg cagcataaaa cccatgaagc caaaatggtt ctaagtaatc cagattgtct
     5821 gtagttgtgt aacgatagcc tgaatactga acagtatatt ttacaatcag cttgccatat
     5881 ccaatggcta tacttccatt tccggaatac atgggtacat aaattaattg cttgccaaca
     5941 gaagcatcat tttcaaagcg ggctttttca ttggtagcct caatataatt tgttgaaacg
     6001 gttaaggctg tgaaccattt tttattgctg tatcgaataa tagtttttgt ttcaattcct
     6061 ttgctttctg ttctgtcaag attttgtgcc gaccaataac cattttccgg tagccatata
     6121 atctgatttt tcattttcct gttaaagagt gtaatctcag tctttgcagt aatgtttttt
     6181 gcaaggttga tttcattaag gcaaaggcca atatcaccgg tttttcctga ttcgggtttt
     6241 agatttttat ttccacccgg ttgccagtac aaatcattta tagttggatt gcgatagaca
     6301 cttgcagcgt ttaatttcaa tgatatattt ttatacaatg caatttctcc tccggcatac
     6361 caggtaaatg gtgcttcctt gttatcatta aattcttttc tgaatgatac tgatggttta
     6421 attttattgt taaagaaatg tgctgtttca ccaataaaaa gcgcaaaaga gtttagctgt
     6481 gcttgctttt tatattcgtc tgctgttgca gtggtgtagt tgttggtgat gcctgcatgt
     6541 atttctctgt gcttacccgg atgaaattta tactcagctt ctgccagcaa tgattttgct
     6601 ctgcttgttg cattcatgtt ctttaaacta tctgaataat gtatgccctg attaaaccat
     6661 gcaacccgaa cagctaattg tccctttgac aac
//
LOCUS       DAOOOL010000124         6615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1188_length_6615_cov_104.854726, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000124 DAOOOL010000000
VERSION     DAOOOL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_6615_cov_104.854726"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PKU82_09565"
     CDS             <1..963
                     /locus_tag="PKU82_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOZ83158.1"
                     /translation="LYNWFIEKLNIYPSRQFEFARRNISYTVMSKRKLLQLVNEKIVD
                     GWDDPRMPTISGVRRRGYTPESIRNFAEVAGISRRENVTDFNLLESCLREHLNKIAVR
                     TMAVINPLKVVLLNYEEHKTEILKAENNPEATEKTYRDIAFSREIFIEQDDFMEHASE
                     GFFRLSIGGMVRLKHAYIIKCENVVKNSDGTIKEIHCTYFPESKSGQDKSGIKVKSVI
                     HWVNAANAVTAEVRLYDKLFTVEAPDAQNEDFKKFINPASLQIFSNAKIEPNMLNYEK
                     DERFQFLRLGYFVEDKYSTSQKRIYNRAVTLKDDWVKEQKKQHK"
     gene            965..1381
                     /locus_tag="PKU82_09570"
     CDS             965..1381
                     /locus_tag="PKU82_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOZ83159.1"
                     /translation="MTIAQIEEEIVSDFELFDDWSQKYEYIIEIGQQLPALDEKFKTD
                     VYKIKGCQSSVWLNASQKDGLLFFEADSDSVFVKGEIALLIKVLSGRTPEEIMSAELK
                     FIDAIGLRQHVAVTRANGLANMIKQIKAYALAHKER"
     gene            complement(1392..2276)
                     /locus_tag="PKU82_09575"
     CDS             complement(1392..2276)
                     /locus_tag="PKU82_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOZ83160.1"
                     /translation="MNISIPTIFKGHGSAVYCLLPKNKNAFYSGSGDHHLIEWNIETP
                     DKGTVVAILPAGIYSLISENNILIAGCGNGEVHCINTQNASALSKAHLHSSYVFDLCF
                     YNQHLYTAGGDGQVVKTDLQLNKLESIHLGNFKIRKLLPHPDGQHILTACGDGTVRIL
                     NVNTLKEEQVIIAHTAGFSVNALCLTPDNKFLLTGSRDARLNVFDVQQNFNLIENIAA
                     HNYAIYDIAFNPSGKIMATASRDKKIKIWNNDLTFITRIEEYPVGHKNSVNTLCWLSD
                     TELITSGDDRNIMLWHLN"
     gene            complement(2279..3511)
                     /locus_tag="PKU82_09580"
     CDS             complement(2279..3511)
                     /locus_tag="PKU82_09580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOZ83161.1"
                     /translation="MYSEQSHKFHGQLFKYATESVVVTNSKGIIVTVNPSALKLFGYT
                     ENEIIGQKIEKLMASRFEASHQLHREGFNKNPHNRAMGIGLDLHAMRKDGSEFPVEIS
                     LSPFSLNDESYVMAFIIDITGRRKVQAQMLEQKNQLEEITRELKQTNERLEAKVLDRT
                     KVLEEALNEIEKSRKELSEALAKEKELNELKSRFLSMASHEFRTPLTTILSSASLITA
                     YAEKDQQDKRIRHVERIKSAVNNLNDILSDFLSLSKIEEGKTKTDFEVFNLKNIVSEI
                     INDLATIKKPGQAINYVHTGKNEVLLDPRLIRNIVINILSNAIKFSDEGKLIEIKSNI
                     GENTTVLEFKDQGIGISKEDQQHLFERFFRGSNATNIQGTGLGLNIVARYVELMNGVI
                     ETESELNKGTAFKITIPC"
     gene            3556..4146
                     /locus_tag="PKU82_09585"
     CDS             3556..4146
                     /locus_tag="PKU82_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOZ83162.1"
                     /translation="MIKSKWINIFVLMKRGDRVIFIFFMLWISTTVKGQSVYSCTNGE
                     VFFRSEAKLELIDAKSQKLEAIVDFQKKTFEFLIPINSFKGFNAELQREHFLDRYMES
                     DKFPVATFKGKIIEDVDLSKPTEITIRAKGKLTIHGIEQERIIKSKISVQGNVVSILS
                     VFSVPLKDHKIKVPRVVQEKIANEVLVQVKATLKRK"
     gene            4143..>6615
                     /locus_tag="PKU82_09590"
     CDS             4143..>6615
                     /locus_tag="PKU82_09590"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOZ83163.1"
                     /translation="MKRIVFILFCIVQTVNGQTPFFKQIALNRDSPNLTVNTLLFCKN
                     GFLWAGTSEGLYRSDGNDHHLLATSDTIKRNVTALFEDNTGVIWIGTKEGEIGFVKDD
                     TLRIIKAFSLKPSVAITSINADTDAIKWLTTAGNGVYYLLDSKFYHIGIEEGLSDDYC
                     YTSAPDKSGNMWIGTDQGLSLCTSKNGKKVLQKLQVADGLCDNIVKKIVIEGSKMFLA
                     TQENGLCIYDFEQNGFLKQKAETVWNYGSIDDIILNKTTNEIWVATESGGLLYFRNPE
                     EKTYSLKQQSAKLFSSVYSVAIDHQNNVWVATDRGLFRSHGQWLTFLEQYAGADINNV
                     HEVAFLDSNQMVVSIKNKIMLISFNTSTIKEWTILPSEKNIDVSGLYIDKEKYIWIGT
                     LGNGVFRINPLKSEIRKIEIPFDYSSILYINGNEKNIWLGTFGGAALIEVEQLNTDNL
                     KYKVRIFSHESPLGNYYVYCVYRDVKGRFWFGTDQNGIVLLDDNKFVAPFSGKFNGRT
                     VYSITGDNNDAIWFSVADEGIVCLNGDSFKLYNRSNGIRDLNITSIAVLDDNSIAIVN
                     KRGIDILNHKSGSVMYLGTENNLDNLNSDINSVAKDSIGRIWFGTVKGMVVLDPKLLK
                     QNTVPELSKLNMIQPIFKNRSGITPVFRHNENTVTFEYAATWFTNPGRILYQYKLDGL
                     NADWIATKDIKQVFPNLPPGKYVFRIRTSIDPQFTFNNESSFSFEIKKPFWQEDWFYF
                     SSLLLAILLVYLFIKVRDARMKKLQQLKNDSIRFQFETLRNQVNPHFLFNSFNTLIDI
                     IEHDKEHAVDYVENLSAFFRNIVTYK"
BASE COUNT         2211 a         1025 c         1258 g         2121 t
ORIGIN      
        1 ctttacaatt ggtttatcga aaaactgaat atttatccta gtcgtcagtt tgaatttgca
       61 agacggaaca tcagttatac tgtaatgagt aagcgcaaac tattgcagtt ggttaacgaa
      121 aaaattgttg atggttggga cgatccgcga atgcctacta tcagtggtgt gcgcagacga
      181 ggttatactc ctgaaagcat tcgtaatttt gcagaagttg ccggaatttc aagacgtgag
      241 aatgttacag attttaattt acttgaatcg tgccttcgcg aacatttgaa taagattgca
      301 gtaagaacaa tggctgtcat taatccattg aaagtagtgt tgttgaatta tgaggagcat
      361 aaaacagaaa ttctgaaagc agaaaataat cctgaagcaa cagaaaaaac atatcgagat
      421 atagcgttca gtcgcgaaat atttattgag caagatgatt ttatggagca tgccagcgaa
      481 ggatttttca gactgtctat tggtggcatg gttcgtttaa aacatgctta cataataaag
      541 tgcgagaatg tggttaagaa tagtgatgga acaattaaag agattcattg cacttatttt
      601 cccgagagta aaagtggtca ggataaatca ggaataaaag taaagagtgt tattcattgg
      661 gtaaatgctg ccaatgcagt aactgctgaa gtgcgtttgt atgataaact gtttaccgtt
      721 gaagcacccg atgcacaaaa cgaagatttt aaaaaattta tcaatcctgc atcactgcaa
      781 atattcagta atgcaaagat tgaacccaac atgctaaact atgaaaagga tgagcgtttt
      841 cagtttttac gtctaggata ttttgttgag gataaataca gcacttcaca aaaacggatt
      901 tacaacagag ctgtaacctt aaaagacgac tgggtgaaag aacagaagaa gcaacataag
      961 taacatgaca atagcacaga tagaagaaga aattgtttca gactttgaat tgtttgatga
     1021 ttggtcacag aagtatgaat atatcattga gataggtcag cagttgccgg cacttgatga
     1081 gaaatttaaa acggatgttt ataaaattaa aggttgccag agtagtgtat ggctcaatgc
     1141 aagtcaaaag gatggacttc tgttttttga ggccgacagc gattctgttt ttgtaaaagg
     1201 tgaaattgcc ttgctgataa aagtattgag cggacgcaca ccggaagaga taatgtctgc
     1261 agagttgaaa tttattgatg ccataggtct gcgtcaacat gttgctgtca cacgtgccaa
     1321 tggtttggcc aatatgataa agcagattaa agcttatgca ttagcacata aagagcgtta
     1381 ataatttacg attagttaag atgccatagc attatatttc tgtcatcacc gctagtgatt
     1441 agctctgtgt ccgaaagcca acaaagcgta ttcacagaat ttttatgacc aacaggatac
     1501 tcttcaatac gagtaataaa agttaaatca ttattccaaa tttttatttt tttatcgcga
     1561 ctggctgttg ccattatttt tcctgaaggg ttaaatgcaa tatcataaat agcataatta
     1621 tgagcagcaa tattttcgat tagattaaaa ttttgttgta catcaaatac atttagtctt
     1681 gcatcacgtg agcctgtcag aagaaattta ttgtcaggtg tgaggcaaag tgcatttaca
     1741 gaaaaacctg ccgtgtgagc aataataact tgttcttctt ttaaagtatt tacatttagt
     1801 attctgacag taccatcacc acaagctgta agaatatgtt gcccatcagg atgaggtaag
     1861 agttttctga ttttaaaatt acccaagtga atggattcta gtttatttaa ctgtaagtcc
     1921 gttttcacaa cttgtccatc accacctgct gtatagagat gttggttgta aaaacataaa
     1981 tcaaagacat aactactatg caaatgtgct tttgataaag cagaagcatt ttgtgtatta
     2041 atacaatgta cttcgccatt gccacagccg gcaatcagaa tgttgttttc tgaaattaaa
     2101 ctgtaaatac cggcaggaag aatagcaact acagtgcctt tatcaggagt ttcaatgttc
     2161 cattctatca gatgatggtc gccactgccg gagtaaaatg catttttatt ttttggtagc
     2221 aggcaatata cagcactgcc atgcccttta aaaattgtag gaatagaaat attcattatt
     2281 agcaggggat agtaatctta aaagcagttc ctttatttaa ttcactttcg gtctcaatta
     2341 caccattcat cagctcaaca tatcgggcaa caatatttaa accaagtcct gtaccttgta
     2401 tgttagtagc attcgaccct cggaagaagc gttcaaacaa atgctgctga tcttccttgc
     2461 tgatacctat tccctgatct ttaaattcaa gtacagttgt attttcacca atgtttgatt
     2521 ttatttcaat gagtttacct tcatcagaaa atttaatggc attggaaaga atattaataa
     2581 caatattccg aatcagccgt gggtcgagta acacttcatt ttttcctgta tgcacataat
     2641 ttatggcttg cccgggtttt ttgatggtag ccaaatcatt gatgatctca cttacaatat
     2701 ttttcagatt gaacacttca aaatcagtct tagtttttcc ttcttcaatt ttactcagag
     2761 aaagaaagtc gcttaaaata tcattcagat tgttgacagc agattttatt ctttcaacat
     2821 gtctgattcg tttatcctgt tgatcttttt cggcataggc agtaatgagt gatgcagatg
     2881 aaagaatagt tgtaagcggt gtacgaaact catgcgatgc catagacaaa aaccttgact
     2941 tgagttcatt caattctttt tcttttgcaa gcgcttcgct taattccttt cttgactttt
     3001 caatttcatt caatgcttcc tccaacactt ttgttcggtc cagaacttta gcctctagtc
     3061 tttcattggt ttgttttaac tcacgagtaa tttcttcaag ttgatttttt tgttcaagca
     3121 tttgtgcctg tacttttctt cggcctgtta tgtcaatgat aaatgccata acatacgatt
     3181 cgtcattcaa agaaaatgga ctcagactaa tttcaaccgg aaattcactg ccatcttttc
     3241 gcatagcatg taaatcgagg ccgataccca tagcacgatt atgcggattt ttattaaatc
     3301 cttctctgtg taattgatgg gatgcttcaa aacgggatgc catcaatttt tctattttct
     3361 gaccaataat ttcattctca gtgtatccaa agagctttaa tgcagatgga tttactgtaa
     3421 caataatacc ttttgaattg gtaacaacaa cgctttctgt tgcatattta aaaagttgac
     3481 cgtgaaattt gtgactttgt tcagaataca taatcaatca tcaaaaacac aaagttagag
     3541 cattaaaagc aaacaatgat aaaatccaaa tggattaata tttttgtttt aatgaaacga
     3601 ggtgatagag taatatttat atttttcatg ctttggattt ctactactgt caaagggcaa
     3661 tcagtttatt cctgtaccaa tggcgaagtg tttttccgca gcgaagccaa acttgagttg
     3721 attgatgcca aatctcaaaa gttggaagcc attgtagatt ttcaaaagaa aacatttgaa
     3781 tttttaatcc ccatcaactc ttttaaagga tttaatgctg aattgcagag agaacacttt
     3841 cttgaccgtt atatggagag tgataaattt cctgtagcaa catttaaagg taagataatt
     3901 gaagatgttg atctctctaa accaactgaa ataacaataa gagcaaaggg caaactaaca
     3961 atacatggaa tagagcagga aaggattatc aagtcaaaaa tttctgttca gggaaatgtt
     4021 gtaagtattt tatcggtttt tagtgttccg ttaaaagatc ataagataaa agtaccgcgt
     4081 gttgttcagg aaaagattgc caatgaagtg ttagtacagg taaaagccac attaaaacgc
     4141 aaatgaaaag aatagttttt attctgtttt gtattgtaca aactgtgaat gggcaaaccc
     4201 cattttttaa acagattgca ttaaacaggg attcacctaa tttaacagtc aatactttat
     4261 tattttgtaa gaatggattt ctttgggctg gcacttcaga aggtctttac cgtagcgatg
     4321 gaaacgatca tcatttactt gcaacaagtg ataccattaa gagaaatgta actgcattat
     4381 ttgaagacaa taccggtgta atttggatag gcactaagga gggtgaaatt ggctttgtta
     4441 aagatgatac attaagaatt atcaaagcat tttctttaaa gccatcagta gccataacat
     4501 ctattaatgc agatacggat gctattaagt ggttaacaac agcaggtaat ggagtttatt
     4561 atttgttgga tagcaagttt tatcatatcg gtattgaaga aggattaagt gatgactact
     4621 gctacacttc tgccccggat aaatcaggta atatgtggat tggaacagat cagggattgt
     4681 cgctttgtac atcaaaaaat ggaaaaaaag ttcttcaaaa gcttcaggtt gcggatggat
     4741 tatgtgataa tatagttaag aaaatagtta tagaaggttc aaaaatgttt cttgcaacac
     4801 aagaaaacgg attatgtatt tatgattttg agcagaatgg ttttctgaaa caaaaggcgg
     4861 agacggtttg gaattatgga tcaatagatg atattattct aaataagaca actaatgaaa
     4921 tttgggttgc aacagaatcc ggtggacttt tatattttcg gaatcctgag gagaagacct
     4981 attctttaaa acaacagtca gcaaaattat ttagctctgt atattcagta gcaatagatc
     5041 atcagaataa tgtttgggtt gcaacagacc gtggtttatt tcgatcacat ggacaatggc
     5101 tgacattttt agaacaatac gctggtgctg atattaataa tgtacatgag gttgcattcc
     5161 tagatagcaa tcaaatggtt gtgagtatta aaaataaaat aatgctcata tcatttaata
     5221 catctactat aaaagaatgg acaattctac cttcagaaaa aaatattgat gtttccggtt
     5281 tatatattga caaagagaaa tatatatgga taggtacttt aggtaacggt gttttcagga
     5341 taaatccttt aaagagtgaa atcaggaaaa tagaaattcc atttgattat agcagtattt
     5401 tatatataaa cggtaatgaa aaaaatatct ggcttggaac atttgggggt gctgctttaa
     5461 ttgaagtaga acaattaaac actgataatc ttaaatataa agtcagaatt ttttctcatg
     5521 aatcaccact aggaaattat tatgtttatt gtgtttatcg tgatgtaaaa gggcggtttt
     5581 ggtttggtac cgatcagaat ggaatagtgc tgttagatga caataaattt gttgctcctt
     5641 tttcagggaa atttaatggc agaacagtat attcaataac aggtgataat aatgatgcaa
     5701 tatggtttag cgtagctgat gaaggaattg tttgccttaa tggagacagt tttaaattat
     5761 ataaccgctc caatggaatc cgcgatttga acataaccag tattgcagtg cttgatgata
     5821 acagcattgc aatagttaat aaaagaggca ttgatatatt gaatcataag tcaggttcag
     5881 ttatgtatct tggaacagaa aacaatctcg ataacctgaa ttctgacatt aattctgtag
     5941 ctaaagatag tattgggaga atctggtttg gcacagtcaa gggtatggta gtattagatc
     6001 caaagttgct gaaacaaaat actgttccgg agttgagtaa attaaacatg atacaaccga
     6061 tatttaaaaa tcgttccgga attacgccag tgttcaggca caatgaaaac acagtaacct
     6121 ttgaatatgc ggcaacttgg tttaccaatc ccggaagaat tctttatcag tataaattag
     6181 atggcttgaa cgcagactgg attgcaacta aagatattaa acaagtattt cctaatttgc
     6241 caccgggtaa atatgttttc agaataagaa cttcaataga tccacagttt acattcaaca
     6301 atgaaagtag tttttcgttt gagataaaaa aaccattttg gcaagaagat tggttttatt
     6361 tttcaagtct gctgctagcg attttattag tttacctctt tataaaagtc agggatgcaa
     6421 gaatgaaaaa gctgcagcaa ctaaaaaacg attctatcag atttcagttt gagacattgc
     6481 gcaatcaggt aaacccacat tttctgttta acagttttaa tacactcata gatatcattg
     6541 agcatgataa agaacatgca gttgattatg tagaaaatct ttctgcattt ttcagaaaca
     6601 ttgtcaccta caagg
//
LOCUS       DAOOOL010000125         6490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1219_length_6490_cov_39.408547, whole genome shotgun sequence.
ACCESSION   DAOOOL010000125 DAOOOL010000000
VERSION     DAOOOL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_6490_cov_39.408547"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PKU82_09595"
     CDS             complement(<1..1454)
                     /locus_tag="PKU82_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83164.1"
                     /translation="MEVKNTYSADSIQVLEGLEAVRKRPAMYIGDVGVKGLHHLVYEV
                     VDNSIDEALAGHCKNISVTIGEDNSITVKDDGRGIPTEMHSKEKKSALEVAMTVLHAG
                     GKFDKDSYKVSGGLHGVGVSCVNALSTHMRTEVHRNGKIYVQEYSIGKPLYSVKEIGT
                     TDYRGTIQTFKPDDTIFITTIYNYDTLASRLRELSYLNKGIRITLTDMREKDEQGNVR
                     SDEFLSELGLREFVEYIDANREKLIPECMFFESDKFGIPIEVAMQYNTSFAENLHSYV
                     NNINTHEGGTHLSGFRRALTRTLKAYAEKEGMLAKLKFDIDGDDFREGLTAVISVKVA
                     EPQFEGQTKTKLGNNEVMGYVDQAVGDMLVHYLEMHPREAKAIVNKVILAATARHAAR
                     KARELVQRKNVLTGTGLPGKLADCSESDPGRCELFLVEGDSAGGTAKQGRDRNFQAIL
                     PLRGKILNVEKAMEYKIYENEEIRNIFTALGIYR"
     gene            complement(1639..2067)
                     /locus_tag="PKU82_09600"
     CDS             complement(1639..2067)
                     /locus_tag="PKU82_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOZ83165.1"
                     /translation="MQKSTIDFRLIKLERNGYHPVCTVAINHIDALMVIDTGASHTVL
                     DKKQAARFLPENKIKLSEVKSSGLGTNDMMSAYATFDYFILGDLVLRRRKTIILDLAH
                     INLAYETMQLPLIDGVIGCDILKKYKASINFSTKKLVINI"
     gene            2199..3716
                     /locus_tag="PKU82_09605"
     CDS             2199..3716
                     /locus_tag="PKU82_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOZ83166.1"
                     /translation="MAILNSIMGWIIKKRMHQIELFMKYPCEVQEELLRKLISTAEHT
                     QWGEHFDYQSIDNFKTFSERVPVVTYDQLQPQVERIMKGEQNILWPTEIKWFAKSSGT
                     TSNRSKFIPVSMESLEECHFKGGKDLLSIYFNNNPDALLFNGKLLSLGGSHSINAFHN
                     ESYYGDLSAILIQNLPFWVQIMRTPEMNIALMDKWDEKIERMAQETIKENVTNISGVP
                     SWTLVLLKRILEITGKKTIAEVWPGLELFVHGAVNFTPYREQFKQIIGKHIQYLETYN
                     ASEGFFGIQDRNDSDEMLLMLDYGIYYEFIPQKHWHEENPKTLTLSEVQIGEQYALVI
                     TTNAGLWRYKIGDTIQFTSISPYRIKISGRTAHFINAFGEELVIDNAEKAITEAGKRT
                     GATVREFTAAPVYFGNKNNGAHEWLIEFSHEPENLDEFTMALDEELKKVNSDYEAKRF
                     KDITLNKPIVHKMPVNTFYKWLEKNKKLGGQHKVPRLANHRKFVDEILNVQNNLS"
     gene            3713..4501
                     /locus_tag="PKU82_09610"
     CDS             3713..4501
                     /locus_tag="PKU82_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83167.1"
                     /translation="MKYIVTLFIIFIYCNTFAQQQKVNEIWNGADAFSMDNLGNLYII
                     KESVVFKADTVGKILLTYSNNNQDRFTYVDAIDPLRIMLFADEFSIISFLDNKLALQS
                     VINLREQNFYDIKAVCNSTDGFWCYDHSQQQIFKYNFTLQKISETQPLALLINNPLTI
                     TSMCANDKWLACLNKNSGILIFDRLGSYVRSIDSKHASCIMLLENMLVYAENNELVFI
                     DLKLNSETSRLKTDINNISSIKWFHNRFYLLADNKIVQIDYKKD"
     gene            4620..5240
                     /locus_tag="PKU82_09615"
     CDS             4620..5240
                     /locus_tag="PKU82_09615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOZ83168.1"
                     /translation="MHIAIAGNIGSGKTTLTSLLSKQFKWEPHYEDVNDNPYLNDFYD
                     DMQRWSFNLQIYFLNSRFQQILKIKKHPKTIIQDRTIYEDAYIFAPNLHAMGLMTTRD
                     FQNYQQLFELMNSFVSPPDLMIYLRGTVPALVKQIEKRGRPYENNIRIDYLKRLNERY
                     EAWISTYELGKLLIIDIDEINFAENKEHLGMIIERVQAELSGLFSK"
     regulatory      5287..5479
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5615..>6490
                     /locus_tag="PKU82_09620"
     CDS             5615..>6490
                     /locus_tag="PKU82_09620"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ83169.1"
                     /translation="MYKNYLLIIFSIICTNIQAQTDTFHIKVVNIEALRQQDATPGLK
                     FEKIDSISLKQFNTESLSDLLSAESGVLIKNYGPGSLASSSIRGGNANQTAVIWNGFN
                     INNPMLGQTDFSIIPEFFADKISMQYGGGSAQWGSGAVGGAVLLNSSTQFNKGFDAEI
                     NVGAGSYGWLRQSFKTNYSNNNWIFKLKVFNEYSPNNFRFQNSITLQKQLQKHARTQG
                     QGLLGEVVYRLNKNGTLSIAAWLDNYNRQIPPLLNQTSVAKQKDDNLRITSQWRQQLS
                     KGQLAVRVAWFNQGIH"
BASE COUNT         2016 a         1196 c         1278 g         2000 t
ORIGIN      
        1 tcacgataga tgccaagggc agtgaagatg tttcttatct cttcgttttc gtagattttg
       61 tattccattg ctttttctac gttgaggatt tttccgcgta gtggaagtat ggcttggaaa
      121 tttctgtcgc ggccttgttt ggcagttcca ccggcagagt ctccctcgac aaggaagagt
      181 tcacatcgtc cggggtcgct ctctgagcag tcggctaatt tgccgggtag tcctgttcca
      241 gtgagtacgt ttttgcgttg caccagctcg cgtgctttgc gtgcagcatg gcgtgccgta
      301 gcagcaagta ttactttgtt gacaatagcc tttgcttcgc gcggatgcat ttcgaggtag
      361 tggacaagca tgtcgccaac ggcctgatca acataaccca taacttcgtt gttaccaagc
      421 tttgtttttg tttgtccttc gaactgcggc tcggcaactt ttactgagat aacggctgtt
      481 agtccttctc tgaaatcgtc accatcaata tcgaatttta atttggcaag catgccttct
      541 ttttcggcat aggcttttaa tgtacgtgtg agtgcacgtc tgaaaccgga aaggtgtgtg
      601 ccaccttcat gtgtgttgat gttgtttaca taggagtgga gattctctgc aaaagaagtg
      661 ttgtattgca tggccacttc aattggaatt ccgaatttat cactctcaaa aaacatacat
      721 tcagggatga gcttttcgcg attggcatca atgtattcaa caaattcgcg caagcccaac
      781 tcacttaaaa actcgtcaga gcgaacattg ccttgctcgt ctttttcgcg catgtcggtt
      841 aatgtgatgc ggatgccttt gttaaggtaa gatagttcgc gcaggcgtga agctaatgtg
      901 tcgtagttgt aaattgttgt gataaaaatt gtgtcatcag gcttgaaggt ttgaatggtg
      961 cctctgtaat cagtagtacc tatctcttta acagaataca atggtttacc aatgctgtat
     1021 tcctgcacat aaatttttcc gttgcggtgt acttctgtgc gcatgtgtgt gctcaatgca
     1081 tttacgcacg atacaccaac accatgtaat ccaccggaaa ctttataaga gtccttgtcg
     1141 aatttgccac cggcatgcag cactgtcatg gctacttcga gtgcagactt tttttctttg
     1201 ctgtgcattt cggtaggaat acctctacca tcatctttaa cagttattga gttgtcttct
     1261 ccaatggtga cactgatgtt tttgcaatgt ccggcaaggg cttcgtcaat tgaattgtca
     1321 actacctcat aaaccaagtg gtgcaatcct tttacaccaa catcgccaat gtacatggca
     1381 gggcgtttcc ttactgcctc caacccctcc aatacctgaa tgctgtcggc cgaataagta
     1441 ttttttactt ccatttcagc taaattttct actgtttgat cactcataac ttttcgtaaa
     1501 ctgtcgtttt ttataattgt tcaaatttac aaaaaataaa ggctgctttc acataaaaac
     1561 ggtgtggaaa tgcacgtttt ttttaacaaa gtcgagcaag ttattaacgc tgtttttaag
     1621 gcttttaatg taaaaaaatc agatgttaat tacaagtttt ttagtagaaa aattgatgct
     1681 tgctttatat tttttaagga tgtcgcaacc tatcacccca tcaatcaatg gcaactgcat
     1741 tgtttcgtag gcaaggttaa tgtgtgcaag gtcgagaatg atagtttttc gtctgcgcaa
     1801 gaccaaatcg cccaatataa aataatcgaa tgtggcatag gcactcatca tgtcgttggt
     1861 gccaagtcct gacgatttaa cttcagaaag ttttatttta ttttcaggta gaaagcgtgc
     1921 cgcctgcttt ttgtctaata ccgtatgcga agcacccgta tcaattacca ttaatgcatc
     1981 tatgtggttg atggcaacag tgcaaacggg gtgataaccg ttgcgctcta attttattaa
     2041 cctgaaatct attgttgatt tttgcatgga acgaaagtac atcaaacatt tctgaaagaa
     2101 attaattaca gattaagttc atttaagttg aagaattaat tttgcaaaac ggaagttgca
     2161 ggaatttttc ttatctttcc gttcgcaaag cactgtatat ggcaatactg aattcgataa
     2221 tgggatggat aattaaaaag cgtatgcatc agattgagct ttttatgaaa tatccatgcg
     2281 aggttcagga agagttgctt agaaaactta tttcaacagc agagcataca cagtggggcg
     2341 aacatttcga ctatcaatct atagacaatt ttaaaacctt tagtgagcgt gtgcctgttg
     2401 ttacttatga tcagttgcag ccacaggtgg aaagaatcat gaaaggcgaa caaaatattc
     2461 tgtggccaac agaaataaaa tggtttgcca aatcatcggg aacaacttca aacagaagta
     2521 agtttattcc ggttagcatg gagtcgctgg aagaatgtca ttttaaaggc ggtaaagacc
     2581 tgctttcaat ttattttaac aacaaccctg atgccttact attcaacggc aaactgcttt
     2641 ctcttggtgg cagccacagc atcaatgcat ttcataacga atcgtattac ggtgatcttt
     2701 ctgctattct gattcagaac cttcctttct gggtacagat aatgcgaaca ccagaaatga
     2761 atattgcact gatggataaa tgggatgaaa aaattgagcg catggcacag gagacaatta
     2821 aggagaatgt gactaacatt tcaggtgtgc ccagttggac attggtgtta ttgaaacgta
     2881 ttcttgaaat tacaggtaag aaaacaatag cagaagtgtg gcctggttta gaactgtttg
     2941 ttcatggtgc tgtaaatttt actccataca gagagcagtt taaacagatt atcggaaaac
     3001 acattcagta tttagaaacc tataatgcct ccgaaggatt ttttggtatt caggacagaa
     3061 acgattctga tgaaatgttg cttatgctcg actatggaat ttactatgag tttattcctc
     3121 aaaaacactg gcatgaggaa aatccaaaaa cgttgacact ctccgaagtt caaatcggag
     3181 aacaatatgc attggtcatt acaaccaatg ccggtctgtg gcgttataaa atcggagaca
     3241 ccattcagtt tacatctatc agtccttaca gaataaaaat cagtgggcga actgcacatt
     3301 ttattaatgc ttttggagaa gaacttgtga ttgacaatgc agaaaaagca attacagaag
     3361 cgggcaaaag aacaggagct acagtgcgtg aatttactgc tgcaccagtt tattttggca
     3421 ataaaaataa tggagcacat gagtggctta ttgaattttc gcatgagcct gaaaacttag
     3481 atgagtttac aatggcactt gacgaagagc ttaaaaaagt taattccgat tatgaggcaa
     3541 aacgatttaa ggacatcacg ctcaacaaac ctattgtgca taaaatgcct gtgaatacgt
     3601 tctataaatg gctggaaaaa aataaaaaat tgggaggaca gcataaggtg ccacgtcttg
     3661 ccaaccacag aaagtttgtt gatgaaatat taaacgtaca aaacaatctg tcgtgaaata
     3721 tattgtaaca ctattcatca ttttcattta ctgcaacact tttgcacagc agcaaaaagt
     3781 taatgaaatc tggaatggtg ccgatgcttt cagcatggat aatctcggca acctctatat
     3841 tataaaagag agtgtagttt ttaaagctga taccgtagga aaaattttac ttacctacag
     3901 caataacaat caggacaggt ttacttatgt tgatgcaatt gacccattgc gaataatgct
     3961 ttttgctgat gaattttcta tcatcagttt tttggataat aaattagcct tgcaatcggt
     4021 aattaattta agagaacaaa atttctatga cataaaagct gtttgcaatt ctacagatgg
     4081 gttttggtgc tatgatcact cacaacaaca aatcttcaaa tataatttta cacttcagaa
     4141 aatttcagaa acacaaccgt tggcattatt aataaacaat ccgctaacga taacgagcat
     4201 gtgtgccaat gataaatggc tggcatgttt gaataagaat tcaggaattt taatttttga
     4261 caggctggga agctatgtaa gaagtattga ttcaaaacat gcatcatgta ttatgctact
     4321 cgaaaacatg ttggtttatg cagaaaacaa tgaactggtt tttattgacc tgaagctgaa
     4381 ttcagaaaca tcaagactga aaactgacat caataacatt agcagtataa aatggtttca
     4441 caaccgtttt tacctgcttg ccgacaataa aatagtacag atagactaca aaaaagatta
     4501 aaactgtaga attataaagg ctattactta agtaactgct tttgccaatg aaattttttt
     4561 tactttagcg cactactttt tgagaactat attatttaaa accagattga tttaattgta
     4621 tgcatattgc aatagcggga aatattggat ccggaaaaac tacactcacc agtttattgt
     4681 caaaacagtt taaatgggaa ccacattacg aagatgtaaa tgacaatccc tatctgaacg
     4741 acttttatga tgacatgcaa cgctggtcgt tcaaccttca aatatatttt ctgaacagcc
     4801 gctttcagca aattttaaaa atcaagaaac acccaaaaac aattattcag gacagaacaa
     4861 tatacgaaga cgcatatatt tttgctccta accttcatgc aatggggtta atgacaacac
     4921 gcgattttca gaactaccaa cagttgtttg agttgatgaa cagttttgtt tcaccacctg
     4981 atttgatgat atatctgcgt ggcaccgttc cggctttggt aaaacaaatt gaaaaacgcg
     5041 gacgacctta tgagaacaat attcgcatag attatctgaa acgtttgaac gaacgttatg
     5101 aagcatggat atcaacttat gaacttggca aactgctgat tattgatatt gatgaaatca
     5161 actttgctga aaacaaagag catctcggaa tgattatcga gcgtgtgcaa gcagaactca
     5221 gcggtttgtt cagcaaataa gctcccttta tttttttatt tatatttgcc accggaagtt
     5281 ctttcattca tttcaaccgg taaaggtttc ttctgtttta gactgaagat ataaaaggga
     5341 atcacgttaa aatcgtgagc tgtagcgcaa ctgtaagtaa cgcataattt atctctgctg
     5401 aatccactgc aaaatttgca ggaaggaata gagttaaaag ttacaagcca ggatatctgt
     5461 ctttactgag gctgtttatt cagccgttga ccatgctttc gcattaaaag cagaagtctg
     5521 tattgataca actttgaacc taggttgtat tactcctcac ttttttattt actgcactgc
     5581 atgtagaaat gcttgacgaa aaaccaagca aaacatgtat aaaaattatc tgctgattat
     5641 tttctctatt atctgcacca acattcaggc gcagacagat acttttcaca tcaaggtggt
     5701 taatattgaa gcattgcgac aacaggatgc tactccggga ttaaaatttg aaaaaattga
     5761 cagcatcagc ctgaagcaat tcaatacgga atcgttaagc gatcttcttt ctgctgaaag
     5821 tggtgttctt attaagaatt acggacctgg ttcgttagct tcttcttcca tcagaggtgg
     5881 aaatgccaat cagactgcag taatctggaa tggatttaac atcaacaatc ctatgctagg
     5941 tcaaaccgat ttttccatta tccctgaatt ttttgctgat aaaataagta tgcaatatgg
     6001 tggcggcagt gcacaatggg gaagtggcgc tgtaggtggt gctgttctgc tcaattcatc
     6061 aacacaattc aataaaggct ttgacgcaga aataaatgtt ggtgccggta gttatggatg
     6121 gttgcgacaa tcttttaaaa ctaactacag caacaataat tggattttta aacttaaagt
     6181 atttaatgaa tattctccaa ataattttcg ttttcaaaat tctattacgc ttcaaaagca
     6241 attacaaaaa catgcaagga ctcaagggca aggtttgtta ggtgaagttg tttacagatt
     6301 gaataaaaac ggcacactaa gcatagctgc atggctcgat aattacaaca gacaaattcc
     6361 tccattactc aatcagacat ctgttgccaa acagaaagat gacaatctaa gaattacttc
     6421 gcaatggcgt cagcagttgt caaagggaca attagctgtt cgggttgcat ggtttaatca
     6481 gggcatacat
//
LOCUS       DAOOOL010000126         6376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1252_length_6376_cov_34.917576, whole genome shotgun sequence.
ACCESSION   DAOOOL010000126 DAOOOL010000000
VERSION     DAOOOL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_6376_cov_34.917576"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..861
                     /locus_tag="PKU82_09625"
     CDS             16..861
                     /locus_tag="PKU82_09625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOZ83170.1"
                     /translation="MVEEILLSQNFVKYKGKVNLIFTSPPFPLNKIKKYGNLNGDQYL
                     NWLSDLAPKLSELLTDDGSIVMEVGNSWQSGKPVMSTLSLKSLIAFQEKGNLELCQQF
                     VWYNSAKLPSPAHWVNVLRIRVKDSFTHIWWMSKTPYPKANNKNVLKEYSQGMKDLLR
                     TQKYNTNKRPSEHSIGLTSFLSNNNGAIPSNVITAANTSVGKNYQEYCVKNNLVLHPA
                     RMPIEIPKFFINFLTNEGDLVLDCFGGSNTTGEAAELLKRKWIAVEPNEDYINGSRGR
                     FIKTK"
     gene            888..2087
                     /locus_tag="PKU82_09630"
     CDS             888..2087
                     /locus_tag="PKU82_09630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ83171.1"
                     /translation="MELKYKPRKWQLIALEKWKENYRGIISVVTGGGKTVFAFLSIME
                     FNKSFPDKKIIIVVPTITLLDQWFISLQEEFGVSKKDISFFSGESKAKIFNKINIVII
                     NTARKILPPIQNKDDYFLIVDECHRAGSPLNGAAIQGNYSATLGLSATPERDYDDGFQ
                     KFMTPNLGNIIYCYNYKDALQDKVICQYELVNVKIDFVEHEQEKYNQITRRISILRKK
                     NDITDPIIKEKLNMLLIQRARISSNALMRMPVTSKIAEENKSERIIIFHESIEGASLI
                     YNTLKKRNQNATIYHSKIGSNLRRDNLRLYRNGSFSILVTCKALDEGMNAPETTVAII
                     ASSTASSRQRIQRLGRVLRPARNKTKAIIYTLYATDSEKDRLIEEYHSLSDDIKVSWL
                     KSSIKNG"
     gene            2080..2856
                     /locus_tag="PKU82_09635"
     CDS             2080..2856
                     /locus_tag="PKU82_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83172.1"
                     /translation="MAKILVNGEWYNELSVGSLYESEFEKLFIDKAPQIFPDYFTVDF
                     KHKVESQHGKGKPDLAIIEKNYRHWFVIEVEKSNHSLENHVLPQVIIFSTADYNDEAA
                     EALKKENESLDIHKLKAMIKGQPPKVLVVVDALKQDWINSLNRWEAMLSVFQVFRSER
                     NRNVFRLNGFTPKSLTGFFSECYLDKIFKRHLVIISPAIIEVPTNGILTINYNDTISE
                     WKRVDMKNQVVLSPLSINPLDASKKYKLHKKENSSYTLTD"
     gene            2869..4386
                     /locus_tag="PKU82_09640"
     CDS             2869..4386
                     /locus_tag="PKU82_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83173.1"
                     /translation="MELKIGLDTISNYKRLSYTPWHAIAEFIDNSTQSFFNNNEVLEK
                     AFKEENEILRVDIVYERDDDVLRISDNSIGMSYEELEGALYVGKPPLNSNGRSKYGLG
                     LKTAACWLGDVWIVKTKKLGNPKEYSVTIDVEKIANGENTLITTEVDVNPDLHYSIIE
                     IKNLNRKFPARTSGKIKAFLKSMYRIDFRNKYLKLTWQGEELKWEEIEERILKDIEGK
                     RYKKLIKVNVLGKDISGWVAILERGSRADAGFSIIQSDRVIKGWPDSWRPSKIYGQDQ
                     GSNDLINQRLFGEIHLDGFTVSHTKDSILWFGDEEEEVEKALSEVCMDYREVAKEYRK
                     NKGALTGPSPVATTAAIDELKKELSSPEMVDEINLTEIPNEEALNETFQDISDNVQNT
                     QMESMNVTIGTLILKVYLNSELSPNDPYVLIEASKPQEIIIIINQSHPHWMQLKDTEV
                     LNYFRHCTYDGVAEWQARNKVSRIDPHTIKLLKDNLLRVQYIIEGNEMEQNTEWQ"
     gene            4720..5262
                     /locus_tag="PKU82_09645"
     CDS             4720..5262
                     /locus_tag="PKU82_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6155 family protein"
                     /protein_id="HOZ83174.1"
                     /translation="MSLNNLKKELKKLDKENLIELVADLYKKNKSVKEYFDFYVNPDE
                     KMLFNKYRIKVYEAFYPKRGDECKLKDGKQAISDFRKLQPSAELLADLMLFYVETGVE
                     YTNEFGDIDQNFYSSLEKTYAAALEFMRKENLLEKFAERTEKVVDDTDGIGWFFHDYL
                     FQVHSEYYDDIDDSDVESSR"
     gene            5724..>6376
                     /locus_tag="PKU82_09650"
     CDS             5724..>6376
                     /locus_tag="PKU82_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ83175.1"
                     /translation="MLPFGWEDVIPIIYSYTITNYCFPIKVIIDKKIVGIGTTIVHND
                     TAWLAHIIVHSDNRKQGIGKKITQSLIETSNSKGCETIYLLATELGESVYKKVGFEIE
                     TEYLIYRCEIMNGTIKNSKNIVAISNDYKKQILSLDRQVTGEDRVLLLEQHLSNGFLI
                     LEEKEVKGFYLPNLGDGLIIANTSSAGQELMKLRLKSKDFAAFPADNLNAAELMRQN"
BASE COUNT         2414 a          948 c         1160 g         1854 t
ORIGIN      
        1 acatactata atggattggt tgaggaaatt ctattatctc aaaattttgt aaaatataaa
       61 ggaaaagtaa atttgatttt tacatctcct ccttttccgt taaataagat aaagaaatat
      121 ggtaatctta atggagatca atatttgaac tggttatctg atttagcacc gaaattaagt
      181 gaattgctga cggatgatgg ttcaattgta atggaagttg gtaattcatg gcaatcaggt
      241 aaacccgtaa tgtcaacact ttctttaaaa tctctgattg cttttcaaga aaaaggtaat
      301 ttagaactat gtcaacaatt tgtttggtat aattctgcaa aacttccatc acctgctcat
      361 tgggtaaatg tacttcgaat aagagtgaag gattccttta ctcacatttg gtggatgagt
      421 aaaacgcctt acccgaaagc aaacaataaa aatgttttga aggaatacag ccaaggcatg
      481 aaagatttat tgcgaaccca aaaatataac acaaacaaac gaccttcaga acatagcatt
      541 ggactaactt cttttttatc aaacaataac ggagcaatac cttccaatgt tataacggct
      601 gctaacacat cagtagggaa aaattatcaa gagtattgtg ttaagaacaa tcttgtttta
      661 catcctgcga gaatgccaat agaaattcca aaattcttta ttaatttttt gacaaatgaa
      721 ggcgatttag ttcttgactg ttttggtggc agtaacacta ctggcgaagc agctgagtta
      781 ctcaaacgaa aatggattgc tgttgaacca aacgaagatt acatcaatgg ttcaagaggt
      841 agatttataa aaacgaaatg atcaccactt ttagaattat ctaaatcatg gaattaaaat
      901 ataaaccaag aaagtggcag ctaattgctt tggaaaagtg gaaagaaaat tacagaggaa
      961 taatcagtgt cgttacaggt ggcggaaaaa ctgtatttgc ttttttatca attatggaat
     1021 tcaataaatc atttccagat aaaaaaataa taattgtagt cccaacaatt actttgcttg
     1081 accaatggtt catcagtttg caagaagagt ttggagtttc gaaaaaggac atatcatttt
     1141 tttcaggtga gagcaaagca aaaattttta acaagataaa cattgtaatc ataaacactg
     1201 caagaaaaat attgccacca atccaaaaca aagatgatta ctttttaatt gttgacgaat
     1261 gtcaccgtgc aggaagtccc ttaaacggtg cagcaattca aggaaattat tcagcgacat
     1321 taggactatc tgcaacacct gaaagagatt atgacgacgg atttcaaaaa tttatgaccc
     1381 ctaacttggg caatataatt tattgctaca attacaaaga tgcacttcaa gataaagtga
     1441 tttgccaata cgaattagtc aatgtcaaaa ttgattttgt tgaacacgaa caagaaaaat
     1501 ataatcaaat tacaagaaga atttcaattc tcagaaaaaa aaatgatatt actgacccaa
     1561 taatcaagga gaaattaaat atgttgttaa ttcagcgtgc aagaatttct tcaaatgcct
     1621 taatgcgaat gcctgttact tccaaaattg cagaagaaaa caaaagcgaa cgaattatta
     1681 ttttccatga gagcattgaa ggtgcatctt taatttacaa tactttaaaa aagagaaatc
     1741 aaaacgcaac gatatatcat tctaaaattg gcagcaatct gagacgtgat aatttgcgat
     1801 tatatcgcaa tggaagtttc agtattcttg taacatgcaa agcacttgat gaaggaatga
     1861 atgcacctga aactacagta gcaattattg cgagttcaac tgcaagttca agacaaagga
     1921 ttcaaaggtt aggcagagtt ttacgaccag caagaaataa aaccaaagca atcatttata
     1981 cactttacgc aactgattca gagaaggata gattaattga agaataccac agtctttcag
     2041 acgacatcaa agtatcgtgg cttaaaagct caataaagaa tggctaaaat tttagttaat
     2101 ggagaatggt ataacgaatt atctgttggt agtttgtacg aatcagagtt tgaaaaactt
     2161 tttatagata aagcacctca aatattccca gattacttta cggtagactt taagcataaa
     2221 gttgaatctc aacatggaaa aggtaaacct gacttagcaa tcattgaaaa gaattatcgt
     2281 cattggttcg tgattgaagt tgaaaaaagt aatcattctc ttgagaacca tgtattacct
     2341 caagttataa ttttttcaac agctgactac aacgatgagg cagcagaagc attaaaaaag
     2401 gaaaatgaaa gtttagacat ccataagtta aaagcaatga ttaaaggaca acccccaaaa
     2461 gtattagttg tagttgatgc tttgaaacaa gattggataa atagtttaaa taggtgggaa
     2521 gcaatgctct ctgttttcca agtttttcgt tctgaaagga ataggaatgt ttttcgctta
     2581 aatggtttta ctccaaaatc attaacaggt tttttcagtg aatgctactt agataaaatt
     2641 tttaaaagac accttgtaat tatatctcct gcaattattg aagtaccaac taacggaata
     2701 ttgacaataa attacaacga cacgatctca gaatggaaaa gggttgatat gaaaaatcaa
     2761 gttgtcctta gtccgctttc aattaatcca ttggacgctt caaaaaaata caagctgcac
     2821 aaaaaagaaa acagttcata taccttaaca gattaaaaat taaaaatcat ggaattaaaa
     2881 atcggtttag acacaatcag caattataag agattatcat acacgccttg gcatgcaatt
     2941 gctgagttta tagataactc aactcaatcg tttttcaaca ataatgaagt tttagagaaa
     3001 gcatttaagg aagaaaacga aattttaagg gttgatattg tttatgaaag agatgatgat
     3061 gttttgagaa tctctgataa ttccatagga atgtcttatg aagaattaga aggagctttg
     3121 tatgtcggca aacctccact caattcaaat ggaagaagca agtatggatt aggtttaaaa
     3181 actgcggcgt gttggcttgg tgatgtgtgg attgtaaaaa caaaaaaatt aggaaatcct
     3241 aaagagtatt ctgttacaat agatgttgag aaaatagcaa atggtgaaaa caccttgatt
     3301 acaacagaag ttgatgttaa tccggatttg cattactcta taattgagat taaaaatttg
     3361 aatagaaaat ttcctgcgag aacaagtgga aaaattaaag cgtttttaaa atcaatgtat
     3421 cgaatagatt tcagaaacaa atacttgaaa ctaacttggc aaggagaaga attaaagtgg
     3481 gaagaaattg aggagagaat attaaaggac atagaaggaa aaaggtataa gaaattaatc
     3541 aaagtaaatg ttcttggaaa agatatttca ggttgggtag ctattttaga aagaggtagt
     3601 cgagccgatg caggtttctc aatcattcag tcagacagag taattaaagg ttggcctgac
     3661 tcgtggagac catcgaaaat ttacggacaa gaccagggtt caaacgactt gattaaccaa
     3721 agattgtttg gtgaaattca cttagacgga tttactgtga gtcatacaaa agacagtatt
     3781 ctttggtttg gtgatgaaga agaagaagta gaaaaagctt tgtcagaagt ttgtatggat
     3841 tacagagaag ttgcgaaaga gtatagaaaa aataaaggtg ctttaacagg accttctcca
     3901 gttgccacca ctgctgcaat tgatgaattg aaaaaagagt taagttcacc tgaaatggtt
     3961 gatgaaatca acttgacaga aatacctaat gaagaagcat taaatgaaac ttttcaagat
     4021 atttctgaca atgttcagaa cactcaaatg gaaagtatga atgtaacaat tggaacgctt
     4081 atattaaaag tttatttaaa tagcgaactt tctccgaatg acccatacgt tttaattgaa
     4141 gcaagtaagc cacaggaaat aattattatc attaatcaat cacatccgca ttggatgcaa
     4201 ttgaaggata ctgaagtttt aaattacttc agacactgta cttacgatgg agttgcagaa
     4261 tggcaagcga ggaataaagt atcacgaata gacccccata ctataaagct tttaaaagat
     4321 aatttactta gagttcaata cataattgaa ggcaatgaaa tggaacaaaa tactgaatgg
     4381 caataaatca aaacacttgc gagtaataca tgtcattttc agacatttta ttttttttgc
     4441 aatacacaaa ataaatcacg tataaaatta aataactatc aaattaccta tccatccagt
     4501 taagacattt ttgaaatctc aacaagttag accaatggat ttaaaaaact ataaattatg
     4561 cagggatttg gggtttgaat agttgaggta ctcaatagtg ttagatttca atttctattt
     4621 catttcagtt tatatgatgt attaatataa attcgccata tccaatcccc ccgataaatt
     4681 ttaactaaca attgaaatga cccaacaact aacaaaacca tgtcactaaa caacctaaaa
     4741 aaagaattaa aaaaactcga caaggaaaat ctgattgaac ttgttgcgga cttatataaa
     4801 aagaataaat ctgtaaaaga gtattttgac ttttatgtga atccggatga gaagatgtta
     4861 ttcaacaaat accgcataaa agtatatgaa gcattttatc cgaaacgggg tgatgaatgc
     4921 aaactaaaag acggcaaaca ggcgatcagc gattttagaa agcttcagcc ttctgcagaa
     4981 ttacttgcag atcttatgct gttttatgtc gagacaggtg tggaatatac aaatgaattt
     5041 ggcgacattg accagaactt ttattcaagt ctggaaaaaa cttatgccgc tgcactggaa
     5101 tttatgcgaa aagaaaacct gcttgaaaaa tttgctgaaa gaactgaaaa ggttgttgat
     5161 gacacagacg gtataggatg gttttttcat gattatttat ttcaggttca ttctgaatat
     5221 tatgatgaca tagatgattc ggacgttgag tcctcccgct gaatttattt ttatataaaa
     5281 gtacaacatt gggagagact caaccggaac aaaaagttga gtcctcccga tagatttatt
     5341 tttacattga agcgcaaata tgggagagac tcaactggaa cagttaatga ttgactacaa
     5401 tcttggctgc acgacacagc agatttacga acttaaaaaa attgacagtg attattttgg
     5461 agaagagtaa aatatactga cgaacagaga acggcccact aatatggttt tagcgtcatg
     5521 cggggtgaag cgtttaaatt caaatgcagt ttttatagtt cttgcagaca gttatgtatt
     5581 ctgaaacccg tccgaacgca aagccggaaa ccgttagcga caacccttaa tcgattcaac
     5641 aaatctaaat tcgaaaacaa atagaagcag aacatatgga catacagaaa ttagaacctg
     5701 ctgacataaa ttttatttcc ggtttgcttc cgtttggctg ggaagatgtt attccgatta
     5761 tttactctta tactataaca aattattgtt ttccaattaa agtaattatt gacaaaaaaa
     5821 tagtcggcat tgggacaaca atagttcaca atgatactgc ctggctggct catattattg
     5881 ttcactctga caacaggaaa cagggtatcg gaaaaaaaat cacacaatct ttaattgaaa
     5941 cttcaaattc caaaggctgt gagacaattt atcttctggc aactgaactt ggagagtctg
     6001 tttataaaaa agttggtttt gaaatagaaa ctgaatattt aatttacagg tgtgaaataa
     6061 tgaacggaac aattaagaat tccaagaata ttgttgcgat cagcaatgat tacaaaaaac
     6121 aaatactaag tttagacaga caagtcacag gtgaagacag agttttactt cttgaacaac
     6181 acctttcaaa cggcttttta attttagaag aaaaagaagt gaagggattc tatttgccga
     6241 atttaggaga cggtttaata atagcaaata caagttcagc aggacaggaa ctaatgaagc
     6301 tacgtttgaa gtcaaaagat tttgctgctt ttcctgctga caatttaaat gcagcagagt
     6361 taatgaggca aaataa
//
LOCUS       DAOOOL010000127         6359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1257_length_6359_cov_47.376110, whole genome shotgun sequence.
ACCESSION   DAOOOL010000127 DAOOOL010000000
VERSION     DAOOOL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_6359_cov_47.376110"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1543)
                     /locus_tag="PKU82_09655"
     CDS             complement(242..1543)
                     /locus_tag="PKU82_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOZ83176.1"
                     /translation="MLTRKMIITVLAGLIPTIVKAQPNINSDLKSLIQKSIAYFPKLK
                     EAELGLQAGKEKLEIAKSAYYPNVMLNAGYNYIDPVSSVSFPVAPGVTRAMQFQPYNN
                     YAATAGFTYPVIDFGRAWFFVKKSNEELEQSKLNIELSKSQLAAQIASVYYSMAYLSK
                     AVAIEDTVLHAMHSYQSVVISRLRNGDALVLDSITTGSAISEEENRKLSLQNLLTKQI
                     ALLQYTTNDTAIVLPEKFDFPQWYIGDDTTSQIQSSFEYKIIDSKLKAQQFDIKMNRS
                     NLFPVVSVNAGMGYRNGYQPDIEKMIFGTQAGLSISAPIFYGNKARNSIKLSKLYLKQ
                     LQQSQENVSSTFRKDVSNVRSDISTLEAQIVNANRQVLQSRTAVELAQSRYRNGLVTY
                     TDVLNALANRQRAELSKLNLEYQLCLARIELARITGVAYYE"
     gene            complement(1553..3142)
                     /locus_tag="PKU82_09660"
     CDS             complement(1553..3142)
                     /locus_tag="PKU82_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOZ83177.1"
                     /translation="MTEPTSYPSGFKRTVIVLTTISAAIMELIDTSIVNVGLTDISGS
                     LGATIEDTSWLITAYAIANVIIIPLTGFLAKYFGRKNYYLASMVIFTVASYLCGESDS
                     LIELIAWRFVQGIGGGALLSTSQSILFDAYQPEKRAMASAMFGMGIVLGPTIGPTLGG
                     YIMEHSSWPLIFFINIPFGIVAVLLTWLYIDRRPEEININRKEIHIDYTGILLLAIGV
                     SSLQYVLERGQTEDWFNSSDIVYLSILSVFGIGFFIWHELRHPHPAIDLRIIQNRNLF
                     ASNVLTFVCGFGLFGSVFIFPVLVQRVLGYTPMEAGLGIVPGAIAAICMMPFIGKGLS
                     SGIRPIYFVMTGFVIFILHGYFSSLANLDSSRQWFIVPQIMRGVGTSFLMVPLINQAV
                     IGISPQKMPYAISLTNMLRQLGGSFGIAIMNTYVAQRVVIHRTDLVANLTAYDPETTE
                     RLNQVAAFTMSKGYSPQQATQAGYQILEGTLTKQSYLHAYLDGFLLISVFLICAMPFI
                     FLLDTKKQSAATLAKVAEEAH"
     gene            complement(3139..4179)
                     /locus_tag="PKU82_09665"
     CDS             complement(3139..4179)
                     /locus_tag="PKU82_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOZ83178.1"
                     /translation="METPEQDKKKISPLFVIAIIVIAVAVFLGVKNLIHNLRYEITDN
                     AQIEAAGVPVISRVSGYVDSLNVADFSQVKEGMPLVSIDSREFVLAVQQAEADVQSAE
                     AELLNAEAGKKNAEAALQVAKTNRDLQKIKTDKAISDFNRDKALYNDGSITQKQIDDS
                     RTNMEAMQKALDVTSDQIKVAVAQQATAQAMVKRADALKETRKAMLDQAKLRLSYCNI
                     SAPATGKTGKCNINTGQFLQAGQILFTIVNNEKFWVIANFKETQLKSLKEGGEANLIV
                     DGFPDKKITGRIASLSEATGARFALLPPDNATGNFVKVTQRVPVKIEIENFNEYKNLL
                     RAGMSVTVEVKK"
     gene            4259..4732
                     /locus_tag="PKU82_09670"
     CDS             4259..4732
                     /locus_tag="PKU82_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOZ83179.1"
                     /translation="MAGKKKDITQHARFNIGRLISIYKRAFDEWATAGLAERGYPQFK
                     ISYFAVLMNINENGKTNKEIAAAAKVSKQAMSRIVKELVEMKLIQVTTHPEDNRSALL
                     KLTAKGRDMVNETKDVMLQLESHYEKAIGKKAFLNYKETTARLINLHWPEFNPYH"
     gene            4821..5360
                     /locus_tag="PKU82_09675"
     CDS             4821..5360
                     /locus_tag="PKU82_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HOZ83180.1"
                     /translation="MNRQAICLIALIAYATTTSAQELVDWQEKRLTWDDFEKRNLPHS
                     RTGAITSSGIYFSYMENAAGLDIIVKAQMDKSRSWVNTHSMHDEVLSHEQMHFDISEI
                     HARLLRQYFALNNKKTARHNPESIYKKYINRLRRMQDDYDRQTNHGNDMYMQMKWEEK
                     IKTMLLDLEQYSGQEMAWR"
     gene            complement(5419..5907)
                     /locus_tag="PKU82_09680"
     CDS             complement(5419..5907)
                     /locus_tag="PKU82_09680"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ83181.1"
                     /translation="MPQKSKIRVIKIVVDTNIIFSVLLNTNGLIGELLFNSEGVYEFY
                     SCDYMRSEIEKHWAKLKRISKLTEQELDESRFQVFSKINFINEKLIPSKIWLTTQAIV
                     EKIDIDDAAFVALSKYLKGGLWTGDKVLHSGLRSKKFKSVFNTTELNKHRELALSKKL
                     NL"
     gene            complement(5919..6140)
                     /locus_tag="PKU82_09685"
     CDS             complement(5919..6140)
                     /locus_tag="PKU82_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83182.1"
                     /translation="MKIRDEMLIERTNKEIIIRLPSNVDTEGLQRLIDFLTYKEATSK
                     SKAKQSEVDKLAKEAKKGWWKRNRSKLIK"
BASE COUNT         1999 a         1236 c         1056 g         2068 t
ORIGIN      
        1 aaaattaggt tcggactctg ttcctttatc ttcattgcca ccaatggcaa taagttttcc
       61 ttttggttta ttcatttatt gatttgagtt atcccccgcc ccaaaaaatg gtattaaaaa
      121 atttgaaagt caaaagtata tatttatcac taaaaagtaa agggttgttt ccccttaaaa
      181 attaactttt taacaatttt ttgtctaaaa agcctattta taccttcgaa gaaagttatt
      241 tttattcata ataagctact cctgtaattc ttgccagttc tatacgtgcc agacaaagtt
      301 gatattcaag atttaactta gacaattcag cacgttgtct gtttgccaaa gcatttaata
      361 catcggtata agttaccagt ccattacgat agcgcgactg cgctaattcc acagcagtac
      421 gcgactgcaa tacctgtctg tttgcgttta caatttgtgc ttcaagtgtt gatatatcac
      481 tcctgacatt gcttacatct tttctgaatg tgctactaac attctcttgc gattgttgca
      541 gttgcttcaa ataaagtttt gaaagtttta tactatttct tgctttatta ccgtaaaaaa
      601 tgggagccga aatacttaat cctgcttgtg taccaaaaat cattttttca atatccggtt
      661 gatatccatt tctataaccc atacctgcat taacactaac cacaggaaaa agatttgacc
      721 gattcatctt aatatcaaac tgctgtgctt taagtttact atcaattatt ttgtattcaa
      781 aacttgattg aatctgactt gtggtatcat cacctatgta ccattgtgga aaatcaaatt
      841 tttccggtaa gactattgct gtgtcattgg ttgtgtattg taaaagcgct atttgttttg
      901 tcaataaatt ctgaagtgat aactttcggt tttcttcttc gctaatggca ctaccggtgg
      961 ttattgaatc aagtactaat gcatcaccat ttctcaacct actaataaca acactctgat
     1021 aagaatgcat ggcatgtaac actgtatctt cgattgctac agcctttgaa agataagcca
     1081 tactgtaata aacagaagct atctgtgctg caagttgtga cttactaagt tctatattaa
     1141 gtttactctg ttccaattcc tcattagatt tttttacaaa aaaccaggcg cgtccgaaat
     1201 ctatcaccgg ataagtaaat cccgctgttg ccgcataatt attgtacggc tgaaactgca
     1261 tagctctcgt cacaccggga gcaacaggaa aggaaacgct acttacagga tcaatataat
     1321 tatatcctgc attgagcata acatttgggt aataagccga cttggctatt tccaattttt
     1381 cttttcctgc ctgcaaaccc aactctgctt cttttaactt agggaagtag gcaattgatt
     1441 tttgaatcaa tgattttaaa tcactgttga tgtttggctg agcttttact attgttggta
     1501 tcaaaccagc cagcactgtt ataatcattt tccttgtcaa catattttta ttttaatgag
     1561 cttcttcagc aacttttgca agtgtagctg ccgattgttt ttttgtatct aaaagaaata
     1621 taaatggcat ggcacaaatg aggaatacac taatcaataa aaagccatct aaatatgcat
     1681 gaagataact ctgctttgtt aaagtgcctt cgagaatctg ataacctgcc tgtgttgcct
     1741 gctgcggaga gtagccctta ctcattgtaa aagctgcaac ttgattcagt ctttccgtag
     1801 tttcaggatc ataagctgtt aaattagcta ccaaatcagt tctgtgtatt actacacgtt
     1861 gtgcgacata agtattcata atagcaatac caaacgatcc acctaattgc cgcaacatgt
     1921 tggttaatga aattgcataa ggcattttct gaggagaaat tccaatgaca gcctgattga
     1981 ttaaaggtac cataaggaat gaagttccaa caccacgcat tatctgaggt acaataaacc
     2041 actggcgtga agaatctaga ttggctaatg atgaaaagta accatgcaaa ataaaaatta
     2101 caaatccagt cattacaaaa taaattggcc ttatgccgct acttaaacct ttaccgataa
     2161 aaggcatcat acaaattgct gcaattgctc cgggcacaat accaagtcct gcttccattg
     2221 gcgtatatcc taatacacgt tgaacaagaa caggaaagat aaaaacagag ccaaacagcc
     2281 cgaagccaca gacaaaggtt aagacattac tggcaaacaa attcctgttt tgaatgattc
     2341 gtaaatctat agcaggatga ggatgacgta gttcatgcca gataaaaaat ccaattccga
     2401 aaacggaaag aatactaagg taaacaatgt cactactgtt aaaccaatcc tctgtttgtc
     2461 cgcgttcaag aacatactgc aaagaactaa caccaattgc caacaacaga attcctgtat
     2521 agtctatatg aatttccttt ctattgatgt tgatttcttc aggtctgcgg tcaatataaa
     2581 gccaggtaag taatactgca acaatgccaa aggggatgtt tataaaaaat atcaatggcc
     2641 aacttgaatg ttccataata taaccaccaa gcgtagggcc aattgttgga ccaagtacaa
     2701 ttcccattcc gaacatggca gatgccatag cacgcttttc cggttgataa gcatcaaaca
     2761 aaatactttg tgatgttgac aacaatgcac caccgccaat accctgaaca aaacgccatg
     2821 ctattaattc aataagtgag tcagattcgc cacagaggta ggaagcaaca gtaaaaatta
     2881 ccatgcttgc cagataataa ttctttcttc cgaaatactt tgctaaaaaa cctgttagtg
     2941 gaatgatgat gacatttgca atagcataag cagtaattaa ccaggaagta tcttcgattg
     3001 tagcacccag actaccgctt atgtctgtca ggcctacatt gacaatagag gtgtcaatca
     3061 actccattat ggctgctgaa atagtagtca gaacaatgac tgttctttta aaacctgatg
     3121 ggtatgaggt aggctctgtc attttttcac ttcaacggta acactcattc ctgcacgaag
     3181 aagattttta tactcattaa aattctctat ctcaattttt acaggaacac gctgcgtcac
     3241 tttcacaaaa tttcctgtag cattgtcagg aggtaatagt gcaaagcgag ctccggtagc
     3301 ttcacttaat gatgctatac gcccagtaat ttttttatca ggaaatccat caactataag
     3361 attagcttca ccgccttctt ttaaactttt aagttgtgtt tctttaaaat ttgcaatcac
     3421 ccaaaatttt tcgttgttaa ctattgtaaa caaaatttgt cctgcttgta aaaactgtcc
     3481 tgtattgatg ttgcatttac ctgttttacc ggttgcaggt gctgaaatat tacaatatga
     3541 aagtctgagt tttgcctgat ccaacattgc ctttctagtt tcctttagcg catcggcacg
     3601 cttcaccatt gcttgtgctg ttgcctgttg tgccactgca acttttatct gatcagaagt
     3661 tacatctaat gctttctgca tggcctccat gttagtcctg ctatcatcta tttgtttttg
     3721 tgtaatactc ccatcattat atagtgcttt atcgcgatta aaatctgata ttgccttatc
     3781 tgttttaatc ttttgcaaat ctctgttggt ttttgctacc tgcagtgctg cttctgcatt
     3841 cttcttgcct gcttctgcat tcaacaattc tgcttctgca ctttgcacat ctgcttctgc
     3901 ttgttgtact gctaatacaa attctcttga atcaatactt actaatggca taccttcttt
     3961 aacctgacta aagtctgcaa cattcagact gtcaacataa ccactgacgc gactgataac
     4021 aggaacaccg gcagcttcaa tttgtgcatt gtccgtgatc tcatacctga ggttatgaat
     4081 cagattttta acgcctaaaa aaacagctac agcaattact ataatagcaa tgacaaatag
     4141 cggactgatt ttctttttat cctgttccgg agtttccata tttttttgaa ttgggcgcca
     4201 aaagtaaaca ttgttgacta tatagtcaag tgtgttgact tttttttatt tttgcaccat
     4261 ggcaggaaag aaaaaagata ttacccaaca tgcaaggttt aatatcggac gcttaatttc
     4321 tatatataag cgcgcatttg acgaatgggc tacggcagga cttgcagaac ggggttatcc
     4381 tcagtttaaa atatcatatt ttgcagtgtt gatgaatatt aatgaaaacg gtaagacaaa
     4441 caaagaaatt gctgctgccg ccaaagtcag taagcaggcc atgagcagaa ttgtaaaaga
     4501 actagtagaa atgaaactga ttcaggttac aacgcatcct gaggataacc gcagtgcttt
     4561 acttaaactt actgctaaag gtcgtgacat ggttaatgaa acaaaagatg taatgctaca
     4621 attagaatca cattatgaaa aagctatcgg caaaaaggca tttttgaatt acaaagaaac
     4681 aactgccaga ctcataaacc ttcattggcc ggaatttaat ccatatcatt aatctgaatt
     4741 acggttaaag ttcaatatgt aagtatttct cttaagtaat aagtcaaata attcatcgtt
     4801 ctcggtaaaa cgatgtttat atgaacaggc aagccatttg tcttattgca ttgatagctt
     4861 atgcaacaac cacttctgca caggaattgg ttgactggca agagaaacgt ttaacctggg
     4921 atgattttga aaaaagaaat ctgccacata gccgaacagg tgcaattacg tcaagtggta
     4981 tttacttcag ctatatggag aacgctgccg gattagacat tatagtaaaa gcacaaatgg
     5041 ataaaagcag atcgtgggta aatactcatt ccatgcacga tgaagttctg tcgcatgaac
     5101 aaatgcattt tgacatttct gaaattcatg cgcgattatt acgtcagtat tttgcattaa
     5161 acaataagaa aactgcgcgt cacaatcctg aaagcattta taaaaaatac atcaataggc
     5221 ttaggcgtat gcaggacgat tatgacagac aaaccaatca tggcaacgac atgtatatgc
     5281 aaatgaagtg ggaggaaaaa ataaaaacta tgctgttaga tttagaacaa tattccggtc
     5341 aggaaatggc gtggaggtag taatagtagt tgttaagtgt ttttaaatta tataatttca
     5401 aataacgtga aattgaattc ataaatttaa cttcttgctt aaagccaatt ctctatgctt
     5461 attcaactca gtggtattga acacagattt aaatttttta cttcttaaac cactatgtaa
     5521 aactttatct cctgtccata aaccaccttt aaggtatttt gacaatgcga caaaagcagc
     5581 atcatcaata tctattttct ctacaattgc ttgcgtagtt aaccatattt tactagggat
     5641 taacttctca ttaataaaat ttattttaga aaaaacttgg aatcgtgatt catcaagctc
     5701 ctgttcagtc aattttgaga ttcgttttaa tttggcccaa tgtttctcta tctcgcttct
     5761 catataatca caactgtaaa attcataaac accctctgag ttaaacaaca attcaccaat
     5821 caacccattc gtgttcagta acacactaaa aataatatta gtatcaacaa caattttaat
     5881 aacacgaatt ttgctctttt gaggcattta cattcgtttc atttgattaa tttgcttcgg
     5941 ttccttttcc accaaccttt ctttgcctcc tttgctaatt tatcaacttc agattgtttt
     6001 gcttttgact ttgaagtggc ttctttatag gttagaaaat caattaagcg ttgcagacct
     6061 tcggtatcaa cgtttgaagg taacctaata ataatttctt tgtttgttcg ctcgattagc
     6121 atttcatctc tgattttcaa tttcaaagat aaataataat ttgaaataat gtgctgtcag
     6181 aaaaactgaa aacctaagtt atcaacttcc gcaagccaaa cagtcatctt tattatcaat
     6241 gctacaactg atttcggcca tagcctcttc cttacttacc gtttgtgtgg tagctacaat
     6301 attatggttg ctgtgtcctt caacggtaaa tttaattgca tcggtagctg ctttagttc
//
LOCUS       DAOOOL010000128         6332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1267_length_6332_cov_190.366258, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000128 DAOOOL010000000
VERSION     DAOOOL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_6332_cov_190.366258"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..414)
                     /locus_tag="PKU82_09690"
     CDS             complement(103..414)
                     /locus_tag="PKU82_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOZ83183.1"
                     /translation="MDSTTEKTLQDLGRKVKMLREAKDISQDELASKLNLVNGHSYIS
                     KIEKGAVNITIEKMCEVLSVLDCEFTVFVKPNFKLSQNKAKKTNEKKGVLKPKKISFT
                     S"
     gene            complement(483..704)
                     /locus_tag="PKU82_09695"
     CDS             complement(483..704)
                     /locus_tag="PKU82_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83184.1"
                     /translation="MSYDKMMKWNSKHIKGTKQTVIMHTNSGFTPSKVFLDSYFEYRN
                     ECKNAGIVPESCEYYYHNKTECRKKLNEN"
     gene            complement(701..916)
                     /locus_tag="PKU82_09700"
     CDS             complement(701..916)
                     /locus_tag="PKU82_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83185.1"
                     /translation="MKNIEIRTQDKATKKTTYRINLSNVIVFLRHDEDKIIVRDDEQV
                     MVEVYHNGELIFSDSKIEFYNKLKNNV"
     gene            complement(913..1491)
                     /locus_tag="PKU82_09705"
     CDS             complement(913..1491)
                     /locus_tag="PKU82_09705"
                     /inference="COORDINATES: protein motif:HMM:NF037233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83186.1"
                     /translation="MANIGFINTNNSLIMANWCSNHVSVTGDNPNVQAFVNEIIELSL
                     KYRKIEEGVLPEYCEYLKDGYLFFDNEIYEKETTDFSADNPCFNYSTQWSPNLAILKC
                     MAEHHKVNIESEYEEMGMQIYGKWTYNDGIENDVWLEQEDFDLYTEYEENYTYTYKGE
                     VYNSEWEVLELILENKIAELKKQHFLTNKETV"
     gene            complement(1452..1616)
                     /locus_tag="PKU82_09710"
     CDS             complement(1452..1616)
                     /locus_tag="PKU82_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83187.1"
                     /translation="MTKIIILDFNTGEAHIYTYDSAEFPEAEDFVNSEGFQSSNCQWL
                     TSDSLTLTIH"
     gene            complement(1634..1924)
                     /locus_tag="PKU82_09715"
     CDS             complement(1634..1924)
                     /locus_tag="PKU82_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83188.1"
                     /translation="MKKTIKPVDVTKIADFYKRQMLRETVNIIQNFGTMYVDTADNKE
                     HVYCLNKVQEMLSKIDEKEKQKFSKRSLNEIKILESVLAKNDCAYIRFIDIY"
     gene            complement(1935..2090)
                     /locus_tag="PKU82_09720"
     CDS             complement(1935..2090)
                     /locus_tag="PKU82_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83189.1"
                     /translation="MFAEDMLQIFDKKTGAYIGLIKRGEPIPEIYTLDKTEQKIEVID
                     VNQLSLF"
     gene            complement(2141..2326)
                     /locus_tag="PKU82_09725"
     CDS             complement(2141..2326)
                     /locus_tag="PKU82_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83190.1"
                     /translation="MQYNLNFLYESGEILVANVSGKKSQMLAVIKADNSIEYIKQNLP
                     TKVKNQIELEANNRVNY"
     gene            complement(2326..3459)
                     /locus_tag="PKU82_09730"
     CDS             complement(2326..3459)
                     /locus_tag="PKU82_09730"
                     /inference="COORDINATES: protein motif:HMM:NF037528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83191.1"
                     /translation="MKNLVATEMFIMGPGGGMEKLEPMKELPLYTKVRKYGAGMLDVD
                     GVVIEPANEHGAQKCVSISESYNVFFTIDRYSRPLSKKFGIGTYYYDDMSKVEKSEVL
                     KYIEKAIKADVERAEKKRIKAEADAKELAELPAKFPHLKAVTENADKKEVKDNLMADL
                     KKNFPGIRFSVTKSNYSTYQIRWTDGPTKKEVNAISDKFEAYSSDITGDFYDYTPSLF
                     NRVFGGFKFVFCNRDMSPEVMQVNKDLEVGSYQDKLDLVFNVWQKTSFPAKFTPTGIK
                     HKDDVMSGNDRADFYEITFDTPAPAEVENTEVMAGEIVFPNGVKIVDYSERAIALIGD
                     TKPIKDTLSGLGGRFNFRLTCGAGWIFPMTKKETVVNALNKLS"
     gene            complement(3483..3953)
                     /locus_tag="PKU82_09735"
     CDS             complement(3483..3953)
                     /locus_tag="PKU82_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOZ83192.1"
                     /translation="MQYLAEEPRTYLKNLPEFSIKYKKGQVKEVKIINAQTAYELLKE
                     LYDADTLEYCESSIVIFLNRNNRSIGWFKVSQGGISGTVVDNRMILATALTAGASSII
                     MSHNHPSGNINPSDIDDKLTSKLKQACDILDIKLLDHIIVTADDYYSYGEQGKI"
     gene            complement(3965..4120)
                     /locus_tag="PKU82_09740"
     CDS             complement(3965..4120)
                     /locus_tag="PKU82_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83193.1"
                     /translation="MRTFKVNFKTASGIAKTYFIDAMDATAALLFFSAEALFHNYDGV
                     SAVINHI"
     gene            complement(4430..>6332)
                     /locus_tag="PKU82_09745"
     CDS             complement(4430..>6332)
                     /locus_tag="PKU82_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83194.1"
                     /translation="ADEFYPYQNVNILSKASSDYPGKVKLNTTTYKYIAYRRYFETPY
                     AVPPFITAIEALCIQRPMLANFKNIMDKMGMLGFLTATVTSPEQNDGEDDTEYWNRCV
                     EYLDKYIYPQMQKNLSSGLVVGFKDAHEFKLEGNNMNVAGADGLVQLVQLMIFAGLKQ
                     DPNMLGRNYSTTETFGRVILEKMLSQIRDYQSLVDQFFSEIYTIALRLKGYEPGYVKV
                     VSKPPMINDRLKDAQAETAEISNVLAKRNAGIIGQKKAANELGYDEPETEDFEIPGQQ
                     TDAGTTPPAEKKKVAPANNSSELIDYYEQKLGVDIPEWSYECNLPHVKKIYNFDGTDF
                     GDKKTNSLSTAYIKETGKVFNKAIDKVSNAVGAKFDKYNENTALNDIQNEVWLTILSK
                     WDSNFVVPMNDVIKRNVEAIYNNFRKDKKAFGNTKTTSANQQKFASADDIPEAVFSLK
                     DYRTIEYMEQHDNMYLGKFITDKDTKARVYKYIEANYIDGNLPIGKNKSEIQKFIDAF
                     REQLSLEAWKIRRIIDTSVTKLKSFGHLRYLSQAEIEKFEVIELIDNVTCPYCEHMDG
                     MEFSVETAVSTIDDEISAGPGKLAATSPFATTYKIDAFKKLTQEDLESSNIVKPPFHP
                     HCRGRLVSV"
BASE COUNT         1620 a         1321 c         1028 g         2363 t
ORIGIN      
        1 ctttacttta tctctaagta taatgaaaaa atcgttataa atcaaaacgg catagcagat
       61 atcctccact atgccgttca tttagcttgt atgtactttg atttaacttg taaaacttat
      121 cttcttgggt ttgagcactc cttttttttc attcgttttt ttggctttat tttggctcaa
      181 tttgaaattc ggttttacga atacagtaaa ctcgcaatcc aatacagata acacctcaca
      241 cattttttct atcgtgatgt ttactgcacc tttttcaatt ttcgagatat aagaatggcc
      301 gtttaccagg ttcagcttac ttgccaactc atcctgactg atatcctttg cctctcgcag
      361 catttttact ttccgaccta aatcctgtaa tgttttttcc gttgttgaat ccataacttt
      421 aatttacctg tgtattgatt tgcacaaata tcgtacttca gtttgatata atcaatagtt
      481 acttaatttt cgtttaattt ttttctgcat tctgttttgt tgtgataata atactcgcat
      541 gattccggaa ctatacctgc atttttgcat tcattcctgt actcaaaata actatctaaa
      601 aaaactttcg acggagtaaa tccactattt gtatgcatta ttactgtctg cttggtgcct
      661 ttgatatgtt tactgttcca cttcatcatt ttatcgtaac tcatacatta ttttttagct
      721 tgttataaaa ttcaatctta ctgtctgaaa atatcaactc tccattgtgg taaacttcta
      781 ccattacctg ctcatcatcc cgaacgataa tcttgtcctc gtcgtgccgt aaaaatacta
      841 tcacgtttga cagattaatt ctgtacgtcg tctttttggt agctttgtcc tgtgttctga
      901 tttctatatt tttcatactg tttctttatt ggttaaaaaa tgttgtttct ttaattctgc
      961 aatcttattc tccaaaatca actccaatac ttcccattca gagttataca cttcaccttt
     1021 gtatgtatag gtataattct cctcatactc ggtatatagg tcgaaatcct cctgctctaa
     1081 ccatacgtca ttttcgatgc cgtcattgta ggtccacttt ccgtaaatct gcatacccat
     1141 ttcctcgtac tcactttcaa tgtttacttt gtgatgttct gccatacact tcaggattgc
     1201 caggttcggg ctccattggg tagaatagtt aaagcatgga ttatccgcgc tgaaatcagt
     1261 cgtttctttc tcataaatct cgttatcaaa aaagagataa ccgtctttta aatattcgca
     1321 gtattcaggc agtacaccct cctcaatttt tctgtacttc aggcttaatt caataatctc
     1381 attaacgaat gcctgtacgt tcggattgtc accggtaacg cttacgtgat tgctacacca
     1441 atttgccata attaatgaat tgttagtgtt aatgaatccg atgttagcca ttggcaatta
     1501 cttgattgaa aaccctcgga gtttacgaaa tcttctgcct ctgggaactc tgcactgtca
     1561 tacgtgtata tgtgtgcttc acctgtgttg aagtctaaaa taataatctt tgtcatgctg
     1621 ctaattttaa ttgttaataa atatctataa atctgatgta ggcacaatcg ttctttgcca
     1681 atacgctttc gagtattttt atttcattca acgaacgttt gctaaatttc tgtttctctt
     1741 tctcatcaat tttgcttaac atttcctgta ctttattcag gcaatataca tgctctttat
     1801 tatctgcagt gtccacgtac atcgttccaa aattttgaat aatgtttact gtttctctta
     1861 gcatctgacg tttataaaag tctgctatct tagttacatc aactggtttt attgtctttt
     1921 tcattactga ttatttagaa taatgataat tgatttacgt ctataacttc tattttttgc
     1981 tctgttttat cgagcgtgta tatttcaggt ataggctcac cacgtttgat taaaccgatg
     2041 tatgctcctg ttttcttgtc gaatatttgg agcatatcct ccgcaaacat atcctctgca
     2101 atcatgttcg ttcgtcggtt cggtataatc ttttgcatgg ttaataattt actctgttat
     2161 ttgcctctaa ttcaatctga tttttcactt tagtaggtaa gttctgtttg atgtattcta
     2221 tcgaattatc tgctttaatt acggccaaca tttggctctt tttaccggat acgttggcta
     2281 ctaatatttc accgctttcg tatagaaaat ttagattgta ttgcattacg ataacttatt
     2341 taaagcgtta acaactgttt ctttttttgt cattggaaat atccaacctg ctccgcaagt
     2401 taatctgaag ttgaaacggc cacctaatcc actcaatgta tctttgatag gcttagtatc
     2461 tccgattagt gcaatggctc tttcagaata gtcaactatt ttcactccat tcggaaatac
     2521 gatttcacct gccataactt ctgtattttc cacttcagca ggtgccggag tatcaaaggt
     2581 tatctcgtaa aaatcagctc tgtcattacc gctcataaca tcgtctttat gtttgatgcc
     2641 tgtaggagtg aacttggcag ggaaactagt cttttgccat acgttaaata ccaaatccaa
     2701 tttgtcctga taactaccta cctccaaatc tttatttacc tgcattactt caggactcat
     2761 atctctatta cagaatacga atttgaatcc tccgaatact ctgttaaaca ggctcggtgt
     2821 ataatcgtaa aaatctcctg tgatgtcgct actgtatgcc tcaaacttgt cgctaattgc
     2881 attcacttct tttttggtcg gtccgtccgt ccacctgatt tgataagtgg agtaattcga
     2941 tttcgttacc gaaaatcgga tgccgggaaa gttcttttta agatcggcca taaggttatc
     3001 tttcacctct tttttgtcag cgttctctgt tactgctttc aggtgtggaa actttgcagg
     3061 taattcggct aattcctttg catctgcttc ggcttttata cgtttttttt cagcacgttc
     3121 aacgtcggct ttgattgctt tctcaatgta ctttaggact tctgattttt ctactttgct
     3181 catgtcgtca tagtagtaag tacctatacc aaatttcttg cttaatggac gtgaataacg
     3241 gtctattgta aaaaatacgt tataactttc agatatggaa acgcatttct gtgcgccatg
     3301 ctcgtttgca ggttctatga ctactccgtc cacgtccaac ataccggctc cgtattttct
     3361 gactttcgtg tataacggta actctttcat tggttccaat ttttccatgc caccccctgg
     3421 gcccatgata aacatttctg ttgctactaa atttttcatg ctattttttt tatttgattt
     3481 gattaaattt taccctgttc accataactg taataatcgt ctgcagtcac tataatatgg
     3541 tctaataatt tgatgtctaa tatatcacaa gcctgtttta gtttacttgt taatttgtcg
     3601 tcaatatcgc ttggattaat attaccgctc ggatgattat ggctcataat tattgatgat
     3661 gctccagcgg ttaatgccgt cgctaatatc attcggttat ctacaactgt acctgaaata
     3721 cctccctgac taactttgaa ccaaccgata cttctgttat ttctattcag gaaaattacg
     3781 attgaactct cacaatactc caatgtgtct gcgtcataca attcttttag cagttcatag
     3841 gcagtctgtg cgttaatgat ttttacttct tttacttgcc cttttttata ctttatagaa
     3901 aactcaggca gattctttaa atacgttctt ggttcctcag cgagatattg catggtacta
     3961 atttttagat gtgattaata acggcagaaa ctccgtcgta attatgaaat aatgcttctg
     4021 cactaaagaa taaaagtgct gcggttgcgt ccattgcatc aatgaaatac gtcttagcga
     4081 ttccggatgc tgttttgaag tttactttaa atgttctcat aattgctgtt tttttaaagt
     4141 gtttattttt gttgttattt caatttgata gtgtaaatct atgcaggata tttgctttgt
     4201 gcaatatcct gcactattat aataccatta ttttcaaaat aaaatcataa gtacgcttat
     4261 ttcaactcgt tacaaactgt tactaaattg ttaaggaaaa taaaaaaagc atttttgtac
     4321 catatttcag gttcaaaaat gctctatcat gttttgaata atatttctaa aaacgaaagt
     4381 tctgaatact acatcttata tctctctttt ttttgtggag tgcaggaaat tagacagata
     4441 ccaaacggcc tcggcaatgc ggatgaaacg gaggctttac gatattgcta ctctccaaat
     4501 cttcctgcgt taattttttg aacgcatcga ttttatacgt cgtcgcaaac ggactcgttg
     4561 ctgccaactt cccgggacct gcactgatct catcgtctat cgtgctgact gccgtttcga
     4621 cgctgaactc catgccgtcc atgtgttcgc aatacgggca cgtaacgttg tcgattaatt
     4681 caatcacctc gaatttttct atctcggcct gtgaaagata ccgcaggtgc ccgaacgatt
     4741 ttagtttcgt tactgaagta tcaataatac gtcgaatctt ccaagcctct aaagataact
     4801 gctcccggaa tgcatcgata aatttctgta tctctgattt gtttttacct ataggcagat
     4861 tgccgtcaat gtagttggcc tcaatgtatt tatacaccct ggctttggta tctttatcag
     4921 taatgaactt acccaaatac atattgtcgt gttgctccat gtattcaatg gtacgataat
     4981 ctttcagcga gaatactgct tcaggaatat cgtctgcaga tgcaaacttc tgttggttgg
     5041 cagatgtagt cttagtatta ccgaatgctt ttttgtcttt cctgaagttg ttgtatatcg
     5101 cctccacgtt acgtttgata acgtcattca ttggtaccac aaagttgcta tcccactttg
     5161 acagtattgt taaccatact tcgttttgga tatcgtttaa ggcagtattt tcattgtact
     5221 tatcaaactt tgctcctacg gcattggaaa ctttatcaat ggctttattg aataccttgc
     5281 ctgtttcttt gatgtatgca gtagataggc tattcgtttt cttgtcacca aagtctgttc
     5341 cgtcaaagtt gtatattttc ttaacgtgtg gcaggttgca ttcgtaactc cattcaggaa
     5401 tatccactcc taatttctgt tcatagtaat cgattaactc gctactgtta ttggccggtg
     5461 ctaccttttt tttttctgca ggtggagtgg tacctgcatc tgtctgttgt ccgggaattt
     5521 cgaaatcttc tgtttccggc tcatcgtacc ctaactcatt ggccgctttc ttttgtccaa
     5581 tgataccggc attacgtttc gccaatacat tcgatatttc tgcagtttca gcctgtgcat
     5641 ctttcagcct gtcattaatc atcggaggtt tactgactac tttcacatac ccaggttcat
     5701 agcctttcaa acgcaatgca atcgtgtaaa tctcgctaaa gaattggtcc actaaacttt
     5761 gataatctct gatttgagat agcatttttt cgaggataac acgcccgaac gtttcggtag
     5821 tggaatagtt acggcctaac atattcggat cttgtttcaa acctgcaaat atcattaact
     5881 gaactaactg taccaatccg tcggctcctg ccacgttcat attgtttccc tccaacttga
     5941 actcatgtgc atctttaaat cctacaacta atcctgaaga taaattcttc tgcatctgcg
     6001 gataaatgta cttgtccaaa tactcaacgc aacggttcca atattcggta tcgtcctctc
     6061 cgtcgttctg ctccggtgac gttactgttg cagtaagaaa accgagcata cccattttat
     6121 ccatgatgtt tttgaagttg gctaacattg gacgctgaat gcataacgct tcaatggccg
     6181 taataaacgg aggaactgca tacggagttt caaagtatct gcggtaagca atgtacttgt
     6241 atgtggtcgt attcagcttt actttgcctg gataatcact actcgcttta ctcaaaatat
     6301 ttacattctg atacggataa aattcatcgg cc
//
LOCUS       DAOOOL010000129         6312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1278_length_6312_cov_143.100048, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000129 DAOOOL010000000
VERSION     DAOOOL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_6312_cov_143.100048"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKU82_09750"
     CDS             complement(<1..355)
                     /locus_tag="PKU82_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83195.1"
                     /translation="MNKIVKYIMLDILKSKVVIAYLVFLMVTGFSIFNLDENTSKGLI
                     SLLNIILFIVPLVCLIYATIYIFNSSEFIELLTAQPVSRKKLITSIYAGVVLSLTLAF
                     LIGIGLPILLFTPDAT"
     gene            complement(348..1061)
                     /locus_tag="PKU82_09755"
     CDS             complement(348..1061)
                     /locus_tag="PKU82_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ83196.1"
                     /translation="MIETININKTFGKLRVLKDISIKLENNSSVALLGPNASGKTTFI
                     KSVLGMVLPESGTIKVNNTDIKNDDSYRSQIGYMPQIGRYPENMNIGQVFEMIMDMRK
                     EQMNHADTDLLDAFNMKSIFHKRMGTLSGGTRQKVSACLAFLFRPKIIILDEPTAGLD
                     PLSSEILKEKVKTEKNNGKLIIITSHILSELDELVSEIIFFREGELMFHKKLEELQLQ
                     TGEKSVSKVITTILKNNNE"
     gene            complement(1058..2317)
                     /gene="nosD"
                     /locus_tag="PKU82_09760"
     CDS             complement(1058..2317)
                     /gene="nosD"
                     /locus_tag="PKU82_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HOZ83197.1"
                     /translation="MKLNRALFVVVKCFLILLFFSGKSQAGVLKIGKKFSIKSIKEGV
                     AKAKPFDTLFIHSGLYKEQNILVEKTLSLIGVDNPVLDGENKFEVLTIDANSVLVTGL
                     TIKNSGYSSMKDLAGIKVMNSRQFLIKNNTLINSYFAIHIANCDNFRIENNTIRGTPG
                     TDEQSTGNAIHLWKCQYASIKNNDISGHRDGIYFEFVENSHIEKNNSYNNLRYGLHFM
                     FSNKDSYIDNKFTDNGAGVAVMFSNHISMYYNLFSMNRGASAYGLLLKEISDGDIKDN
                     MFENNTTGVLMEGTNRLHITKNSFYKNGYALRVQASCSNNTVHDNNFNGNTFDVATNG
                     SLVLNNFDGNYWDKYEGYDLKRDGVGDVPYRPVSLFSMVTENIPIASIFIRSLIVFLL
                     DRSEKIIPSITPENLVDNKPLMKPVAL"
     gene            complement(2304..2732)
                     /locus_tag="PKU82_09765"
     CDS             complement(2304..2732)
                     /locus_tag="PKU82_09765"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOZ83198.1"
                     /translation="MRWTFIFISLLIVSCTTKPEPLVAGKDACVHCKMPIADTRFGAE
                     IISNKGRIYKFDDINCMLQWSRQSSNVDAGSAKLYVVNYFDQIDLMNAADAVYLKSDN
                     LRTPMNSGFAAFATKEEAEKLLKDDGGVLVDWNFVQNEIK"
     gene            complement(2745..3320)
                     /locus_tag="PKU82_09770"
     CDS             complement(2745..3320)
                     /locus_tag="PKU82_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83199.1"
                     /translation="MTKINGVSRVIIAISSLLIAISFFVPIWRIDLFAPQYPEGLVMK
                     IWLDKLTGDVEVINGLNHYIGMKKISVEMFPEFAFLKYIVAAFIVYGLVIAITGSRKI
                     LFSYLVVSVICGILALYDFYQWGYDYGHNLDPNAPIKIPGLVYQPPILGHKQLLNFDA
                     HSMPDVGGYIVIGSALICAIIWAMGLRKSKS"
     gene            complement(3358..3921)
                     /locus_tag="PKU82_09775"
     CDS             complement(3358..3921)
                     /locus_tag="PKU82_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOZ83200.1"
                     /translation="MNTKLLMGVMFAASVAFSCNPPAAENQQSTTTTEETQAGGQEAV
                     IDDDSQKDIVKIAVGSADHSTLVAALKQADYVTSLSNAGPFTVFAPTNEAFNKLPAGT
                     VDDLMKPENKAKLQDILEYHVSLGVFKPEMLTDGQTINQANLKNITISNKDNKIMINN
                     QANIIATIPAANGVIHVIDAVLLPPDK"
     gene            complement(4002..5924)
                     /gene="nosZ"
                     /locus_tag="PKU82_09780"
     CDS             complement(4002..5924)
                     /gene="nosZ"
                     /locus_tag="PKU82_09780"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HOZ83201.1"
                     /translation="MPSCKPKNVSTATSGDAAQKAYVAPGKYDEFYNIVSGGFSGQMS
                     IYGLPSGRLFRVIPVFSVDPEKGYGYSEESKAMLNTSHGFVPWDDLHHISLSETDGIH
                     DGRWAFGNANNTPRIARIDLKTFRTAEIIEIPNSAGNHSSPFITENSEYVVAGTRFSV
                     PMTNGTTDVPINTYKENFKGTVSFINVDKESGKMGIAFQILLPGVDFDLSRAGKGKSH
                     GWFFFSCYNSERANTLLEVNASKFDKDFIMAVNWKKAEEYVKAGKGVKKSVSYAHNTF
                     DDKTHTATSTIEKEVIMLDPKDCPDMVYLMPCPKSPHGCDTDPTGEYIVGSGKLAAVI
                     PVFSFTKMQEAINAKNFEGDYDGLPVLKYDAVLHGEVKKPGLGPLHTEFDANGNAYTS
                     FFVSSEIVKWRLSDLSVLDRVPTYYSVGHLCVPGGPTKKPWGKYVIAYNKITKDRYLP
                     TGPELTQSAQLYDISGDKMKLILDFPTIGEPHYAEAIPAELLSKNSTKIYKIEENHHP
                     YVTKGEKEAKVERKGNEVHVYMSSIRSHFVPDNIEGVKLGDVVYFHVTNLEQDWDVPH
                     GFAVKGANNAELLIMPGETSTLMWKPDRVGVFPFYCTDFCSALHQEMQGYIRISPANS
                     NVPLTFSTGTNQPPIN"
     gene            complement(6013..>6312)
                     /locus_tag="PKU82_09785"
     CDS             complement(6013..>6312)
                     /locus_tag="PKU82_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ83202.1"
                     /translation="PKLDKQMADAGKSVYDVKCSACHKLTDEKLVGPGWKGVTGKRSV
                     EWIMNFSTNPDEMLDKDAIAKGLLEECLVRMPNQGLSDDDARHVYEYMRENDGVK"
BASE COUNT         1980 a         1196 c         1024 g         2112 t
ORIGIN      
        1 ctgtggcatc cggtgtaaaa agcaatatag gcaagccaat tcctattaaa aaagccaatg
       61 ttaaagaaag aacaacacct gcataaatac ttgtaatgag tttttttctg gagacaggtt
      121 gtgctgtaag taactcaata aattcagatg aattaaaaat atagatggta gcataaataa
      181 ggcagaccaa aggaacaata aagagaataa tatttaataa gctgattaat ccttttgaag
      241 tattttcatc aagattaaaa atactgaatc ctgttaccat caaaaaaaca agataggcaa
      301 ttacaacctt gcttttcagt atgtcgagca tgatatattt aactatctta ttcattattg
      361 ttttttaaaa tggttgtaat aactttagaa acagatttct cccctgtttg aagttgaagc
      421 tcttctaatt ttttatgaaa catcaattcc ccttccctga aaaagataat ttccgaaacc
      481 aactcatcaa gttcactaag aatgtgtgat gtaataatta tcagtttgcc attatttttt
      541 tctgttttga ctttttcttt aagtatctct gatgataaag ggtcgagtcc ggcagttggt
      601 tcatcaagaa taataatttt aggacgaaac agaaatgcaa gacaggcact cactttttgt
      661 cttgtgccac ctgataaagt tcccatacgt ttatgaaaaa tagatttcat attaaacgca
      721 tcgagtaagt cagtgtctgc atgattcatt tgctctttac gcatatccat aatcatttca
      781 aacacctgac caatattcat attttcggga taacgaccaa tctgcggcat atatccaatc
      841 tgactgcgat agctgtcgtc attcttaata tctgtgttgt taacttttat tgtgcccgat
      901 tcgggcaaca ccattcctaa aacagattta ataaatgttg ttttaccaga agcattaggt
      961 ccaagtaacg caacagagct gttgttttca agctttattg atatatcttt taataccctg
     1021 agtttaccaa aagttttgtt gatgtttatg gtttctatca cagtgctaca ggtttcatta
     1081 atggtttatt atctactaaa ttttccggtg taatgcttgg aataattttt tcgctgcggt
     1141 caagtaaaaa cacaattaaa cttcttataa atattgaagc aataggaata ttttctgtaa
     1201 ccatcgaaaa cagacttacc ggtctgtagg gtacatctcc aacaccatct cttttcaaat
     1261 cataaccttc atatttatcc cagtagttac catcaaagtt atttaaaacc aatgagccat
     1321 tggttgcaac atcaaatgtg tttccgttaa aattattatc gtgaactgtg ttgttactac
     1381 agcttgcctg aacacgcaag gcatatccat ttttatagaa actgtttttt gtgatatgaa
     1441 gtctgtttgt tccttccatc aaaactccgg ttgtgttgtt ttcaaacatg ttgtctttta
     1501 tatctccgtc agatatttct tttaacaaca aaccataggc agaagctcct ctgttcatac
     1561 taaataaatt ataatacatt gaaatatgat tggaaaacat gacagcaaca ccagcaccat
     1621 tatcagtaaa tttattgtct atgtatgaat ctttgtttga gaacataaaa tgtaagccat
     1681 aacgtaaatt attataactg ttattttttt caatatgtga gttttctaca aattcaaaat
     1741 atataccatc tctatgacca gaaatgtcat tattttttat agatgcatac tggcacttcc
     1801 aaagatgaat agcattgccg gtagattgtt catcagttcc cggtgttcca cgaatggtat
     1861 tattttctat tctgaaatta tcacaattgg caatatgtat tgcaaaataa gagttaatga
     1921 gcgtattgtt tttaattaga aactgccggc tgttcatgac tttaataccg gccaaatctt
     1981 tcatagagga ataacctgaa tttttaattg ttagtccggt aactaataca ctgtttgcat
     2041 caattgttaa gacctcaaac ttattttctc catcaaggac aggattgtca acaccaatga
     2101 gtgacagcgt tttttctact aggatgtttt gctctttata aagacctgaa tggataaaaa
     2161 gtgtatcaaa aggtttagct ttagcaaccc cttctttaat ggacttaatt gaaaattttt
     2221 ttccgatttt taaaacacct gcctgcgatt tgcctgaaaa aaacagaaga atcagaaaac
     2281 attttacaac aacaaacagt gctctattta atttcattct gaacaaaatt ccagtcaact
     2341 aatacaccac catcatcttt caatagtttt tcggcttctt ctttagtagc aaaagcagca
     2401 aagccgctat tcattggcgt acgaaggttg tcgcttttta aatagacggc atcagctgca
     2461 ttcatcaaat caatctgatc aaaataattt acaacatata attttgcaga acctgcatct
     2521 acgttagatg attgtctgct ccattgcaac atgcagttaa tatcatcaaa tttatatata
     2581 cgtcctttat ttgatattat ttctgcacca aaacgagtat ctgctatggg cattttgcag
     2641 tgtacacaag catctttacc ggcaactaat ggttcaggct ttgttgtaca tgatacaata
     2701 agaagagaaa taaaaatgaa tgtccacctc atacacttta ttatttaaga tttacttttt
     2761 cttaatccca ttgcccagat aatagcacag ataagtgcag agccaatgac aatatatcca
     2821 cctacatcag gcatagagtg tgcatcaaaa ttcaacaatt gtttatgtcc taaaattgga
     2881 ggctgataaa ctaatccggg aattttaata ggtgcattag gatcaagatt atgtccataa
     2941 tcataacccc actgatagaa atcgtagagc gcaagaatgc cacatataac agaaactacg
     3001 aggtaactaa acaatatttt acgactaccg gtaatggcta tcactaaccc ataaacaatg
     3061 aatgcagcta caatatattt caggaatgca aattccggaa acatctctac gctaattttt
     3121 ttcatgccta tatagtggtt caaaccatta ataacctcta cgtcacccgt tagtttatcg
     3181 agccatattt tcattaccaa cccttcagga tattgtgggg caaacaaatc aattcgccag
     3241 atgggtacga aaaatgaaat agctataagt aatgaagaga tagcaatgat aactcttgat
     3301 acaccgttta tttttgtcat aaaaatttgt atttaatttt acctcctaag aattaaatta
     3361 tttatcaggt ggcaataaaa ctgcatcaat aacatgtatc accccatttg ctgcaggaat
     3421 ggtagcaatg atatttgcct gattgttaat cattatttta ttgtctttat ttgaaatagt
     3481 aatgtttttc aaattagcct gattgattgt ttgtccatct gtaagcatct ctggtttaaa
     3541 aacaccaagt gaaacatgat actcaagaat atcctgaagt ttagctttat tttcaggttt
     3601 cattaaatca tctactgtac ctgctggtaa cttattaaac gcttcattag taggtgcaaa
     3661 aacagtaaat gggcctgcat ttgaaagaga agttacatag tcggcctgtt tgagtgctgc
     3721 tactaaagta gaatggtctg ctgagcctac tgcaatctta acaatatctt tttgcgaatc
     3781 atcgtctata acagcttcct gtccgccagc ttgtgtttct tcagtggttg ttgttgattg
     3841 ttggttttct gctgcgggag gattacagct aaatgctact gatgcagcaa acattactcc
     3901 cattaataat tttgtgttca taataatctt tagtttttat aaagcacctc gttggtagtg
     3961 aacaacgagg tgcgttgtgt tatatttatt aaattaaaga tttagttgat agggggttga
     4021 tttgtacctg tgctgaaagt caacggtaca ttagaattag cgggactgat tctgatgtag
     4081 ccttgcattt cctgatgtaa tgcagaacag aagtctgtac agtagaatgg gaatacacct
     4141 actctgtccg gtttccacat taaggttgat gtttctcccg gcatgattaa taactcagca
     4201 ttattagcac cttttacagc aaagccgtgt ggaacatccc agtcttgctc taagttagtt
     4261 acgtggaagt aaacaacatc acccaattta acaccttcaa tattatcagg aacaaagtgt
     4321 gaacggatag aagacatata aacatgcact tcatttcctt ttctttcaac tttagcttct
     4381 ttttcacctt tcgttacgta agggtgatga ttttcttcaa tcttataaat ttttgtagaa
     4441 tttttagata gtaattctgc aggaatagct tctgcatagt gtggctcacc aattgttggg
     4501 aagtctaata taagtttcat cttatcgcca ctgatatcat aaagctgtgc actctgtgtc
     4561 aactccggac ctgttggcag ataacgatct tttgtaattt tattataagc aattacatat
     4621 ttaccccaag gctttttagt tggaccacct gggacgcata agtgaccaac agaataataa
     4681 gtaggtacac ggtcaagtac tgataaatca ctcaaacgcc atttaacaat ctctgatgat
     4741 acaaagaatg aagtataggc atttccattt gcatcaaact ctgtgtgcaa tggtccaaga
     4801 cctggctttt taacttcacc atgtaatact gcatcatatt ttaaaacagg taagccatca
     4861 taatcgcctt caaaattttt agcgtttatt gcttcttgca tttttgtaaa tgagaataca
     4921 ggaataactg cagccaattt accgctacca acaatatatt cacctgttgg atcagtatca
     4981 caaccgtgtg gtgattttgg acaaggcatc agataaacca tatcaggaca atcttttgga
     5041 tcgagcatga ttacctcttt ttcaatagtt gaagtagctg tgtgtgtttt atcatcaaaa
     5101 gtattgtgag cgtaagaaac actcttttta acaccctttc cggcttttac atattcttcg
     5161 gcttttttcc agttaacagc cataataaag tctttgtcaa acttcgaggc attaacttca
     5221 agtagcgtat ttgctctttc agaattgtaa caactgaaga agaaccaacc gtgagattta
     5281 ccttttcctg cacgagacaa gtcaaagtca acaccgggta gtaatatctg gaatgcaatt
     5341 cccatttttc ctgattcttt atctacattg ataaagctta cagtaccttt aaaattctct
     5401 ttataagtat taatcggaac gtctgttgtt ccattagtca tcggtacact aaaacgggta
     5461 ccggcaacta catactcaga attttcagta ataaatggtg aggagtgatt tcctgcactg
     5521 ttaggaattt caatgatttc tgctgttctg aaagttttca aatcaatacg tgcaatacgc
     5581 ggagtgttgt ttgcattgcc aaaagcccag cgaccatcat gaataccatc agtttctgat
     5641 aaagaaatgt ggtgcaaatc atcccaggga acaaatccat gtgaagtgtt tagcatagcc
     5701 ttagattctt cgctgtagcc atatcctttt tcaggatcta cagaaaaaac aggaattacc
     5761 ctgaacagtc ttccagaagg caatccataa atactcattt gcccactaaa acctcctgat
     5821 acgatgttgt aaaactcatc atatttaccg ggagctacat aggccttttg tgcagcatca
     5881 cccgatgtgg ctgtactcac atttttaggc ttgcacgatg gcattacagc aattgtaatt
     5941 acagctgctg atgccaataa tacattttta atttccgatt gtttcataat atttaattta
     6001 ttttatagtt ccttacttta caccatcatt ctcacgcatg tactcataga catgacgtgc
     6061 atcatcatca ctcaaccctt gattaggcat acgcaccaaa cattcttcca atagtccctt
     6121 tgcaatagca tctttatcta acatttcatc agggtttgta gaaaagttca taatccactc
     6181 tacagatctt tttccggtaa cacctttcca gcccggtccc acaagtttct catcggttaa
     6241 cttatgacag gctgaacatt ttacatcata aacggattta cctgcatctg ccatttgttt
     6301 atcaagtttt gg
//
LOCUS       DAOOOL010000130         6183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1323_length_6183_cov_33.459040, whole genome shotgun sequence.
ACCESSION   DAOOOL010000130 DAOOOL010000000
VERSION     DAOOOL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1323_length_6183_cov_33.459040"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6183)
                     /locus_tag="PKU82_09790"
     CDS             complement(<1..>6183)
                     /locus_tag="PKU82_09790"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ83203.1"
                     /translation="LSGAGLTAGANITGYGYNKQNNGQPAGANGWTINVYLKNSSATT
                     LASGTSWSTMISGAVLGYTATITSSNMPSATGYWVWPISGFTYTGGAIECYIEWFPAT
                     AMASPYTTNSFTWQYTTSGNQAMGTSSSTAIPSSQSSWTTQARFYNTQIDYTTGPCTG
                     TPSPGNTTTSASPVCPSATFSLGLQNPTTGSGVTYQWQSSPDNITYTNISGATNAAYS
                     LTQSADTWYQCIVTCTNSGQTTTSTPLQVTTNPFYNCYCASGATSTADEEIFGVTVGS
                     MSNSSTCTTTACGTGSVNSNYSNYKGCVTGPTVSQGDNVPFSLDLGYCGSFAYSNMAA
                     IYIDYNQNGSFADAGEQVYTKPYAAGALPSLVVTGSFIVPFTALTGTTGMRIVYVESS
                     SISSCGTFSWGETEDYNITITASTNCSGTPAPGNTLADQTSVCGGQTINLTLQNSVTG
                     SGVTYDWQSSPDGVTWTSTGGTNPFYSTTQNSATWYQCVVTCTNSGQSATSTAVQVLQ
                     NPFMSCYCASGATSTADEEIFGVTVGSMSNSSTCSTIGCGTGSGVGYANYKGCLTGPT
                     VFLGDLVPFSLTLGYCGSYAYSNTAAIYIDYNQNGSFADAGETVYTKPYGSGTVPSWV
                     VSGNFTVPMTATTGTTVMRIVYVESSTISPCGTFSWGETEDYEINIIAPTPCSGTPAP
                     GNTLSDQTTACSNTTINLSLQNIVAALGITYDWQSSPDGVTWTSTGGTQPFYSTTQFT
                     ATWYQCVVTCSNSGLSGTSTPVQVTMSPFYNCYCTSGLGGSCSINSLSSVAIATTTLN
                     NVTGGCTGTYNSFPASGNTTASLVPGVPYTMTAGCISGSNTQVAIWIDYNQNGIYETT
                     EYTLINGNIPSGGTGPGSFTIPLTALSGSTGMRVRSDWQGTTAWTSADACTNRTWGET
                     EDYTITVLVPNSCTGTPATSVTTASVASVCPGGSVNLATNPFYTDGGLTFDWQSSTDG
                     ITYSSTGVTTQNYNVTGFSVTTWYQCVVTCTNSGLSTTSTPVQVTLNPFYNCYCTANL
                     HSFSTPCIDEIDLNTLSNNTAAAGCALPAYSYQSATTTVLKGSTYTFTRTASGTGVWT
                     GLWIDYNHSGTFDASEYIQVSSTSTATLVNSISLTIPLTALDGNTGMRIRQRTVNMTA
                     TDACTQNFGSGETEDYTINIQTPAPCSGTPAAGNTVSSAPSVCTSVAFNLSISTSYNL
                     AGIIYQWQSSTDGITYTNTSGTLPTYSATQSVATWYQCIVTCTNSGLSITTTPVQVTM
                     SPFFNCYCASSATSTADEEIFGVTVGSMTNTSTCTTTACGAGSTNQMYSNYHGCVTPA
                     TIIQGTSVPFSLDLGYCGATAYSNTAAIYIDFNQNGLFTDAGETVYTKPYGAGVVPSS
                     VYTGNFTVPMTAVPGTTGMRIVYVESSVVNPCGTYSWGETEDYDIVIAAAAACSGTPA
                     ATISGPSNTCAGVPFNLNAGPVGFSGLSYLFQVSTNGGVTWTDLGIASANSIYTISSQ
                     TVTSQYQVIVTCSNSSSSDTSAVITVTQNLPSACYCVPTTTGATTYNIINFTTTGGLA
                     NINNSSTGTANYENFSAMTATVNPGSSFNYSIDVAGGSNYGRAIWIDFNEDGVFQSTE
                     QVVSSTSYVYPPLTGSISIPLSVTSGTKRMRILASYTPSNPSNACANSGLGQYEDYSL
                     NIMPPVCIFAPTYPADAASGCPDVITNTVPLSWPALSGATGYDVYFGTTNPPTTLVSN
                     NQPGLTFNADVSGGAPIYYWMIVPQISGGGTSCNVWSFTVSPAPVPVASSGGDVCSGN
                     DIFLSSDNVAMGQLTGNTYSWTGPNGFNSTLQNPSISSPSAAYSGTYSVIVTNQYGCT
                     AGASTSLSVNPNPTLTIDSVQNVSCLGGSDGIIYVSASGGLAPYSYTSDFINFNTNGI
                     MNNLPTGASIVYAADGNACQAQIVGNLTAISTAPPSQSVVVTPTIIGMPAYACPGTTA
                     NLSIPAVPGATMYIWDGPFGTFFNGNPMNQSPFTTTTPSVQITFSSSTTSFVSVGVQA
                     ANGCGASLRKIQKVRYQISTPKEITGATTMCANTNGTYTIAPVT"
BASE COUNT         1778 a         1257 c         1264 g         1884 t
ORIGIN      
        1 tctgtcaccg gtgctatagt gtaagtaccg ttggtattag cacacatggt cgttgctccg
       61 gtaatctcct ttggggtgct tatctgatat ctaaccttct gtatcttacg tagggatgct
      121 ccgcaaccgt tggctgcctg aacgcctaca cttacaaacg atgtggttga agaactgaat
      181 gtaatctgta cacttggtgt tgttgttgta aaaggtgact ggttcatcgg attaccatta
      241 aagaaagttc cgaatggacc atcccatata tacatcgttg ctcccggaac tgcaggtatg
      301 ctcaaattgg ctgttgtacc gggacaagca taggcaggca tgcctattat cgttggtgtt
      361 accactaccg attgtgatgg cggtgccgtg cttatcgctg ttaaattacc tacaatttgt
      421 gcctgacatg cattgccatc tgcagcatat acaatacttg ctccagttgg cagattgttc
      481 attattccat ttgtattaaa gtttataaaa tctgaagtat aactataagg tgctaagccg
      541 cctgacgctg atacgtaaat aataccatca cttccgccta aacaacttac attctgtaca
      601 gaatctattg ttaacgttgg atttggattt acactcagac ttgtgcttgc tcctgctgta
      661 caaccatact gatttgtaac tataactgaa taggtaccgc tgtaggctgc acttgggctg
      721 ctgatagatg gattttgtaa tgtgctgtta aaaccattag gtcctgtcca tgaataggtg
      781 ttgccggtaa gttgtcccat tgctacgtta tcagatgaaa ggaagatatc attacctgag
      841 catacatcgc ctcctgaact tgctactgga acaggtgccg gtgatacagt aaatgaccat
      901 acattacatg aagtacctcc acctgatatc tgtggtacta tcatccagta atagattggt
      961 gctccacctg atacatcggc attgaaagtt aaacccggtt gattattaga aaccaaagta
     1021 gttggaggat ttgtggtacc aaaatacaca tcatagcctg tggctcctga aagtgcaggc
     1081 cagcttaatg gaacagtatt ggtaattaca tcaggacatc cgcttgctgc atctgcagga
     1141 taggttggtg caaaaataca taccggtggc atgatgttca gactataatc ctcatactga
     1201 cccaaaccgc tgtttgcgca tgcattagat ggattacttg gagtatatga tgccaagata
     1261 cgcatacgtt ttgttcctga agttacactt aatggaattg atatacttcc tgtaagtggc
     1321 gggtaaacat aagatgtgct acttactacc tgctctgttg attggaatac tccatcttcg
     1381 ttaaagtcaa tccagatggc acgaccataa ttactacctc cagctacatc aatactatag
     1441 ttaaatgatg aacctggatt aacagttgct gtcattgcac tgaagttctc ataatttgct
     1501 gtacctgtgc tgctgttgtt aatatttgcc aaaccacctg ttgttgtaaa gttaatgatg
     1561 ttataggttg ttgctccagt tgttgtaggt acacaataac atgcactagg gagattctgt
     1621 gttacagtaa taacagctga tgtatcgcta ctgctgctgt tagaacaagt aactattacc
     1681 tgatactgac tggtaactgt ttggctgctg atggtataaa tactattagc actagcaata
     1741 cctaaatcag tccatgtaac tccaccatta gtagaaactt ggaacaagta acttaatcca
     1801 ctaaagccca caggacctgc attaaggtta aaaggaacgc ctgcacaagt gtttgaagga
     1861 ccactgattg ttgctgccgg tgttccggag catgctgctg ctgctgcaat aactatgtca
     1921 tagtcttctg tctcacccca tgaatatgtt ccgcatggat ttactacact actttctaca
     1981 taaacaatac gcattcctgt tgtaccggga acagctgtca tcggaactgt aaaattgcct
     2041 gtataaaccg aacttggcac aactcctgca ccataaggtt tggtataaac tgtttcgcct
     2101 gcatctgtaa acaaaccatt ttggttgaag tcaatataaa ttgctgctgt attactatat
     2161 gcagttgcac cgcaataacc taaatcgagt gagaaaggta ctgatgttcc ctgaatgata
     2221 gtagccggtg ttacgcaacc atgatagtta gaatacattt ggttagttga acctgctccg
     2281 catgcagtgg ttgtacaagt agatgtatta gtcatagaac caactgtcac accaaatatt
     2341 tcttcatcgg ctgtagaagt agctgaagaa gcacaatagc aattaaagaa tggactcata
     2401 gttacctgta caggagtagt tgttatagac agaccactat tagtacatgt tactatacac
     2461 tgataccatg ttgccacaga ttgagttgct gagtaggttg gcaatgttcc tgaagtattg
     2521 gtataagtaa ttccatctgt tgacgactgc cactgataaa taattcccgc caaattataa
     2581 cttgtagata ttgaaagatt aaatgcaaca cttgtacaaa ctgaaggtgc agacgaaact
     2641 gtatttcctg ctgctggtgt tccactacat ggtgctggtg tttgaatatt gattgtgtaa
     2701 tcttctgttt cacctgatcc aaaattctga gtacaagcat cagttgctgt catattaact
     2761 gtgcgttgac ggatacgcat tcctgtattg ccatccaatg cagtaagtgg tatcgtcaat
     2821 gaaatactgt tgaccaatgt tgctgtagaa gttgacgata cctgaatata ttcacttgca
     2881 tcaaacgttc cactatgatt atagtctatc cataagcctg tccatactcc agttcctgac
     2941 gctgttcttg tgaaggtata tgtgcttcct tttagaaccg ttgttgtagc tgactgatag
     3001 gaatatgcag gcaacgcaca tcctgctgct gcagtgttat tactcaatgt atttaaatca
     3061 atttcatcta tacaaggcgt actgaatgag tgcaggtttg cagtacaata gcaattatag
     3121 aatggattta atgtaacctg aacaggagtt gaagtagtac ttaatccact gttggtgcaa
     3181 gtaacaacac attgatacca tgtagttaca gagaatccgg ttacattgta attttgtgta
     3241 gtaacacctg tcgaagaata agttattcca tcagttgagc tctgccaatc aaatgtcaaa
     3301 ccaccatcgg tataaaatgg attggtagct aaattcacac ttcctccagg acatacactt
     3361 gcaacagatg ctgttgtaac agatgttgca ggtgtgccag tacatgagtt tggaaccaaa
     3421 actgtaatag tataatcttc tgtttcgccc caggtacgat tggtgcaagc atctgcactt
     3481 gtccatgctg ttgttccctg ccagtcacta cgtacacgca ttcctgttga gccgcttaat
     3541 gcagttaatg gaattgtaaa cgatcctggt ccagttcctc cacttggaat atttccattg
     3601 attaatgtat attctgttgt ttcataaatt ccattctggt tatagtcaat ccagatagct
     3661 acctgcgtat tgctaccgga aatgcaacct gcagtcatgg tatatggaac accgggaacc
     3721 aaacttgctg ttgtatttcc agatgccgga aatgaattat atgttcccgt acatccacca
     3781 gtgacattat ttaatgttgt tgttgcaatt gccacacttg aaagcgagtt tattgagcaa
     3841 cttccgccaa gtccgctagt acagtagcaa ttataaaatg gactcatagt tacctgaacc
     3901 ggagtagaag ttccactcaa tccactgtta gagcaagtaa caacacattg ataccaggtt
     3961 gccgtaaact gggtagttga ataaaatggc tgtgttccac cggtacttgt ccaggttaca
     4021 ccatcaggag atgattgcca gtcgtaggta atacctaaag cagcaactat attctgaagt
     4081 gataaattaa tggttgtatt tgaacatgcc gttgtctgat cagaaagtgt attaccaggc
     4141 gctggtgtac cactacatgg agtaggtgct attatattaa tttcatagtc ttctgtttca
     4201 ccccatgaaa atgttccaca aggactgatt gtgctacttt caacataaac aattcgcata
     4261 acagttgtac ctgttgttgc agtcattggc accgtaaagt ttcctgaaac aacccaactt
     4321 ggtactgtac cggaaccata aggctttgta taaacagttt cacctgcatc tgcaaacgaa
     4381 ccattttggt tgtaatcaat atatattgct gctgtattgc tatacgcata gctgccacaa
     4441 taccctaatg ttaatgagaa aggtaccaaa tcacctaaaa atactgtagg accggttaaa
     4501 cagcctttat aatttgcata gccaacgcct gaacctgttc cacaacctat agtgctacaa
     4561 gttgagctat tgctcataga tccaaccgtt acaccaaaga tttcttcatc tgcagtagat
     4621 gttgcacctg atgcacagta acaagacata aatggatttt gcaaaacctg tacagctgtt
     4681 gaagtagcac tctgcccact attggtacaa gtaactacgc attgatacca tgtagctgaa
     4741 ttttgtgttg ttgaataaaa cgggtttgtt ccacctgtac tagtccaggt tacaccatca
     4801 ggtgatgatt gccaatcgta ggtaacgcct gagcctgtaa cgctattttg taaagtcaaa
     4861 ttaattgtct gaccaccgca aacagaagtt tgatctgcca atgtattgcc gggtgcaggc
     4921 gtacctgaac agttggtaga tgctgtaata gtgatattat agtcttctgt ttctccccac
     4981 gaaaaagtac cacacgaact gattgagcta ctttctacgt aaacaatacg catgcctgtt
     5041 gtgcctgtta aggctgtaaa tggtacaata aaacttcctg taactactaa gcttggtagg
     5101 gcacctgctg cataaggttt ggtatatact tgctcgcctg catctgcaaa tgatccattc
     5161 tgattgtaat ctatgtaaat tgctgccata ttgctataag caaagctgcc gcaataacct
     5221 aaatctaatg aaaaaggcac attgtcacct tgagaaactg taggtccggt tacacaaccc
     5281 ttataatttg aatagttaga gtttaccgat cctgtcccac aagcagtagt tgtacaggtt
     5341 gatgaattac tcattgatcc aacagtaaca ccaaaaattt cttcgtctgc tgttgatgta
     5401 gctccggaag cacaataaca gttgtaaaat ggatttgtag taacttgtaa tggtgttgaa
     5461 gtagtagttt gaccactgtt agtacaggta acaatacatt gataccatgt atcggcagat
     5521 tgtgttaaag aataagcagc attggtagct ccactgatat tagtatatgt aatattatca
     5581 ggtgatgatt gccattgata tgttacgcca ctgccagttg taggattctg taatcctaaa
     5641 gagaaagtag cacttggaca aacaggactt gctgatgttg tagtattacc cggtgaaggt
     5701 gttccagtac aagggcctgt tgtataatct atttgagtat tgtaaaatct tgcttgggta
     5761 gtccatgatg actggctgga aggaatagca gtactactgc ttgttcccat agcctgattg
     5821 ccagaagttg tatattgcca tgtaaatgaa tttgtggtat atggagatgc catagctgtt
     5881 gcaggaaacc actctatata acattcaatt gccccaccgg tataagtaaa tccagaaata
     5941 ggccataccc aatatcctgt tgccgaaggc atgttacttg atgtaatagt tgctgtatat
     6001 cctaatacag ctccactaat catggtggac caactggtac cgctggctaa ggttgtagca
     6061 gatgagtttt ttaaataaac attaattgtc catccgttag caccggcagg ttgtccgttg
     6121 ttttgtttat tataaccata tccggtaata tttgctccag cagtaagacc agcacctgat
     6181 aat
//
LOCUS       DAOOOL010000131         6181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1324_length_6181_cov_41.516650, whole genome shotgun sequence.
ACCESSION   DAOOOL010000131 DAOOOL010000000
VERSION     DAOOOL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_6181_cov_41.516650"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5601)
                     /locus_tag="PKU82_09795"
     CDS             complement(<1..5601)
                     /locus_tag="PKU82_09795"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOZ83204.1"
                     /translation="MTKTQYNSKAKSASFWRWIMCFMFLLGIWTTGAQAQTTVAVGPT
                     TVSASSYFYGPVYRSSASSTFHYSMYSYIYSASEIAAAGIVSGSFINDIQFNLNAAAT
                     LSGAHTGNFEIWIQNSSATDQTSGTSWSTITGTATQVVAQTWNNTNPGSGGWKTFTFN
                     SPFAYTGQSLIVSVRWVLIPSGGSPYASGAFNWQYYTGPLITPAAAIGAASGSAQSGS
                     TALSTVSYGGNRRPLTQMSYTAGSPCSGTPTPGNTNSNPTSACVGQTISLSLQNLTMG
                     SGVTYDWQNSPDGVTWTSTGGTLPTYSTTQVASTWYQCVVTCTNSGLSTTSTPVQVSM
                     NPFYSCYCDPTYSSGTGFGDYISLVAIPTTTLNNPTTGAPSPYFTVYPQSGNTTCSLV
                     AGANYTMNVAGGTYSSCYIRGWIDYNQDGNFDASETIGVSGNVGSLTTGSFNFIVPVT
                     ALTGTTRMRIRSSDTSPGPSSSQVCGAANSTYGEAEDYLITLLAANACTGTPAASATT
                     ASVSGVCSGGNVDLATNPVYTDGGLTFDWQSSPDGITYSSTGVTTQTYNVTGFSATTW
                     YQCVVTCTNSGLSTTSTPVQVLLNSFYNCYCASIATYTGDEDIFNVTFGTLNNTSTCS
                     STGPGVGSVNQRYANYASGTGAPAAPNVIKGSTVPFSVQIGTCGSNYSNMTKIYIDWN
                     QNGSYADAGEEVYVSPTYTSGPHTETGNILIPMTAVAGTTGMRVITVETTSASSITPC
                     GSYGWGETEDYLVNVIQPVPCSGAPAPGLTVASVNPACFVNPLVLSLQNPMTDLGLTF
                     NWLTSPDGVTWSSTGVTTSTLTVNQSAATYYMCVVTCTNSGQSGNSTALLVNQTVCYC
                     ETPVYSYNSGYNMCTYYDYIGNVTFAGINNTTVCDNTPPYYNYYSSQTATVNRTGSYQ
                     VTISATAGGASFQLYKVYIDYNDNGSFDDAGEMVYTSGFISTATNFTQGNIVIPMTAP
                     LGTHRMRVRSTNPLNTNIDANSCSDDGYLGEVEDYNIVVNPAPPCSGTPAPGATLSDK
                     ALVCSSTTINLSLQNATPGTGVTYQWQSAVDVAFTTPVNLGTAATQTATQTATTYYRC
                     LVTCSGNTGISTPVLATSTTACYCTNPTTGSDGCALGLYINNVTFAGINNTSGCFSAV
                     PYTTPFYSFFPTQLATAQQGNTYAFSASAPNNPGSYYQWYGVWIDYNDNGSFDDAGEL
                     VLSSIAGASTTNNQLTGNITIPLTATAGQHLMRVRTGNVSVAINSCSDNSVSGEVEDY
                     LIDIIAAPQCSTPAPGNTIASATSVCANENLDLSLQNNNIALGLSYQWQVSTVGASGP
                     WTNVGTNANTYTATQSVASWYQCIVSCSYTSISTTSTPVGVTMNSVASCYCVAGASID
                     GCGTGNEYVSNVTFNTINNTSTCSGVALQYQDFTAISTNVQAGQSYTASVMITNYFSS
                     DQATVWIDFNQNGSFADAGEQFNLSGGASGVAFTGSITIPGNAVTGVTRMRVRGNYSG
                     AMNPCGITSYGEVEDYSLNITPPVCIFAPTYPADAGSACPDAITNTVQLSWPSLPAAT
                     GYDVYFGTNNPPTTVVSPNQPGLTYVANVTSGTQYYWMIVPQMPGGGTSCSVWSFTVS
                     PPPMPIASSGGDVCEGSDIFLSADNVDPGQLTGNTYSWTGPNSFSSALQNPSINSPSA
                     AYSGTYSVIVTNQYGCTASASTSLSVNPNPTITIDSVQNVSCLGGSDGIIYVSGSGGL
                     APYSFTSDFVNFNATGVMNNLPTGSSIIYAADGNACQAQITGNLTAISTAPPSQSVVV
                     TPTIIGMPAYACPGTTANLSIPAVPGATKYIWDGPFGTFFNGNPMNQSPYTTTTPSVQ
                     ITFSSSTTSFVNVGVQAANGCGASL"
BASE COUNT         1806 a         1233 c         1220 g         1922 t
ORIGIN      
        1 cagtgatgct ccgcagccat tggctgcctg tactcctaca tttacaaacg atgttgttga
       61 tgaactgaac gtgatctgca cacttggtgt tgttgttgta tatggcgact ggttcatcgg
      121 attaccatta aagaaggtac cgaatgggcc atcccagata tacttcgttg caccgggaac
      181 tgcaggtatg cttaagtttg ccgtagtacc cggacaggca taggctggca tgcctataat
      241 cgttggtgtt acaactaccg attgtgatgg tggtgccgtg cttatcgctg ttaaattacc
      301 cgttatctgt gcctgacagg cattgccatc tgcagcataa ataatacttg accctgtagg
      361 caaattattc atcactcctg ttgcgttaaa gttcacaaaa tctgacgtga agctataggg
      421 tgctaagcct cctgatcctg atacgtaaat aataccatca ctgccgccta aacaacttac
      481 attctgtact gaatctattg ttatcgttgg atttgggttt acactcaggc tggtgcttgc
      541 acttgccgta cagccatact gattcgttac tattactgaa taggtgccac tataggctgc
      601 gcttgggctg ttgatagatg gattttgtaa ggcgctgcta aagctattgg ggcccgtcca
      661 tgaataggtg ttgcccgtta actgaccggg atctacattg tctgccgaca agaagatgtc
      721 actgccctca cacacatcgc ctcctgaact tgctataggc ataggtggtg gtgatactgt
      781 aaatgaccat acgctacatg aggttcctcc gcccggcatt tgtggtacta tcatccagta
      841 atactgtgtt ccactagtta cgttagcaac ataagttaaa cccggctgat ttggagatac
      901 aactgttgtt ggaggattgt ttgtgccgaa atacacgtca taacctgtag ctgcaggtaa
      961 agatggccag cttaactgaa ctgtattggt aatggcatca ggacatgcac ttcctgcatc
     1021 tgcaggataa gttggtgcaa aaatacatac cggtggtgta atattcagac tatagtcttc
     1081 tacttcacca taagatgtaa taccacatgg gttcattgca ccggaataat ttccgcgaac
     1141 tctcattctt gttactcctg taactgcatt tccgggaata gttatacttc ctgtaaatgc
     1201 tacaccagat gcaccgcctg aaagattaaa ctgctcacct gcatctgcaa aggatccgtt
     1261 ctgattaaag tcaatccaaa ctgttgcctg atcacttgag aaatagtttg taatcattac
     1321 agaagctgta taagattgac cagcctgaac attcgttgaa attgctgtga aatcctgata
     1381 ctgcagtgca acaccagaac atgttgatgt gttattgatt gtgttaaatg ttacattact
     1441 cacatactca ttacctgttc cacaaccatc tatagaagct cctgctacac aataacaact
     1501 ggcaacagaa ttcattgtta caccaacagg tgtagaagta gtagaaatac ttgtataaga
     1561 acaactaaca atacactgat accaggaggc aaccgattgt gttgcagtat aggtatttgc
     1621 gtttgtaccc acatttgtcc atggtccgct ggcaccaact gtagatacct gccattgata
     1681 agataaacct aatgcgatat tattattctg caaactcaaa tccaagttct catttgcaca
     1741 tacagaagta gctgatgcta ttgtattacc cggtgcaggc gttgaacact gtggagctgc
     1801 aataatatca atcagataat cttctacttc accacttaca gagttgtcag aacatgaatt
     1861 gattgcaact gatacattac cggttctaac acgcatcagg tgttgaccag ctgtagcagt
     1921 taaaggaatg gtaatatttc ctgtaagttg attattggtt gttgaagctc ctgcaatact
     1981 tgacaacacc aattcaccgg catcgtcaaa acttccgttg tcattataat caatccaaac
     2041 accataccat tgatagtatg agccgggatt gtttggtgca gaagcagaga aagcataggt
     2101 attaccttgt tgcgctgttg ccaactgggt tgggaagaag ctatagaatg gtgttgtata
     2161 tggtactgca ctaaaacaac cactggtatt gtttatacct gcaaaagtta cattgttgat
     2221 atacaaacca agagcacaac catcactacc ggttgtggga ttggtgcagt aacatgcagt
     2281 tgttgatgtt gctaataccg gagttgaaat tccggtgtta ccactacaag ttacaagaca
     2341 acgataatat gttgttgctg tttgtgttgc tgtttgtgtt gcagctgtac ccaaatttac
     2401 cggagtggta aatgcaacat caactgctga ctgccattga taagttacac ctgtacccgg
     2461 agtagcgttc tgcaaactca ggttaattgt tgtagatgaa caaacaagtg ctttatcaga
     2521 taatgtagca cccggtgccg gtgtaccgct acaaggaggt gcaggattaa ctacaatatt
     2581 ataatcctct acttcaccaa gataaccatc atccgaacaa gaattggcat caatgtttgt
     2641 atttaatgga tttgttgatc taactctcat acggtgtgta cctaatggag cagtcattgg
     2701 aataacaata tttccctgag taaagttagt tgcagtactg atgaaacctg aagtgtaaac
     2761 catttcacct gcatcatcaa agctaccatt gtcattgtag tcaatataaa ctttatacaa
     2821 ttggaatgat gcgccaccgg ctgttgcaga aattgttacc tgataacttc ctgttctgtt
     2881 aaccgttgct gtttgtgatg aataataatt ataatatgga ggcgtattat cgcagacagt
     2941 agtgttatta atacctgcaa atgttacatt tccaatataa tcataataag tacacatatt
     3001 gtagcctgaa ttgtagctat atacaggagt ttcacaataa caaaccgttt ggttaaccaa
     3061 taatgcagta gagtttcctg attgccctga attggtacat gtaacaacac acatgtaata
     3121 agtagctgct gactgattaa ctgtcaatgt tgaagttgtt acacctgtag aagaccaggt
     3181 aacaccatca ggagaagtta accaattaaa tgttaatcct aaatcggtca ttggattttg
     3241 cagacttaaa actaaaggat taacaaaaca tgcaggattt acacttgcaa cagttaaacc
     3301 cggtgcaggt gccccgctac atggtacagg ttgaataaca ttaaccaaat aatcttctgt
     3361 ttcgccccat ccataggaac cacaaggcgt aatgcttgag gcagaagttg tttcaactgt
     3421 aattacacgc attcctgtag ttccggcaac agcagtcatc gggattaaaa tatttccggt
     3481 ttctgtgtgt ggaccacttg tatatgttgg agaaacataa acttcttctc cggcatcggc
     3541 ataagaacca ttttgattcc agtcaatgta aattttggtc atgttactgt agttgcttcc
     3601 gcaagtacct atctgaacag agaaaggtac ggttgaaccc ttgataacat taggtgcagc
     3661 cggtgctcct gtgccactgg catagtttgc ataacgctgg ttcacagagc cgacaccggg
     3721 accggtactt gaacatgttg aagtgttatt taatgttccg aatgttacgt taaatatatc
     3781 ttcatcgcct gtatatgtag caatagatgc acaataacaa ttgtagaatg aattcaataa
     3841 aacctgtacc ggagttgaag ttgtacttaa tccactgttt gtacaagtaa caacacattg
     3901 ataccatgtt gttgcagaaa atccagttac attgtaagtc tgtgttgtaa ctcctgttga
     3961 agaataagta attccatcag gtgaactctg ccagtcaaat gtcaagccgc catcggtata
     4021 gacagggttt gttgcaagat caacattacc acctgaacat actccgctta ctgatgctgt
     4081 agttgctgat gctgcaggtg ttcctgtaca agcatttgct gctaataaag ttattaaata
     4141 atcttctgct tcaccataag tgctattggc tgctccacat acttgcgaac ttgatggccc
     4201 tggtgaagta tcacttgaac gtattctcat tctggtagta ccggttaaag cagttaccgg
     4261 cacaataaaa ttaaaacttc cagtggttag tgaaccaaca ttaccagaaa cgccaatagt
     4321 ttctgatgca tcaaaattac catcctgatt ataatcaatc caacctctta tataacaaga
     4381 tgaataagta ccaccggcaa cattcattgt ataatttgca ccagctacaa gtgaacatgt
     4441 tgtatttcct gattgcggat agactgtaaa gtaaggtgaa ggtgctcctg ttgttggatt
     4501 atttaaggtt gttgttggaa ttgccactaa tgaaatataa tctccaaatc cagtgccaga
     4561 agaataagtt gggtcacaat agcaactata aaatggattc atactaactt gaacaggagt
     4621 ggaggttgta cttaatccac tgtttgtaca agtaacaaca cattgatacc aggtagatgc
     4681 aacttgtgtg gtagaataag ttggtaatgt accacctgta cttgtccatg taacaccatc
     4741 aggtgagttt tgccaatcat aagttacccc tgagcccata gttagatttt gcagtgataa
     4801 tgatattgtt tgaccaacac aagcagatgt aggattagaa tttgtattac ccggagttgg
     4861 ggttcctgaa caaggagagc cggctgtata agacatttgt gtcaatggac gtctgtttcc
     4921 accatatgat actgtactta aagcagtgct acccgattga gctgaaccac ttgctgcacc
     4981 aattgctgct gcaggtgtta ttaatggacc cgtataatat tgccagttaa atgcaccact
     5041 tgcataaggt gatcctcctg aagggattaa tacccatcgt acagaaacaa ttaatgattg
     5101 acctgtgtac gcaaaaggtg aattgaaagt aaaagttttc caaccaccgg aaccgggatt
     5161 tgtgttattc caggtttgcg caactacttg cgttgctgta cctgttattg tggaccaaga
     5221 agtgcctgag gtttgatctg ttgcactgct gttttgtatc cagatttcaa aatttccagt
     5281 atgcgcacca cttaatgtag ctgctgcatt taaattaaac tggatgtcat taataaaact
     5341 tccgcttact atacctgctg ccgctatttc cgaagcacta tagatatagc tgtacattga
     5401 ataatgaaat gtactagatg cagatgacct ataaacaggt ccatagaagt aactacttgc
     5461 tgatacagta gtaggaccta ccgctactgt tgtttgcgct tgtgcgcccg tagtccatat
     5521 tccaagcagg aacatgaaac acataatcca cctccagaat gaagcagact ttgctttact
     5581 gttgtactgt gtttttgtca tgataatttg atttttggtt taacttatgt tttgattttg
     5641 ttttctgatt ttcaaaaact aattctgccg ttgttgtgaa aaataattac aaattgtagt
     5701 tagcactcgt ctttttaaca gcatataagt ttggttttcg ccttcaatat tattcatcat
     5761 tttttaatat tccaaattat ttttaaaata aaatcttctg ttatatatta taaatatcac
     5821 tttattattg attatcaatg ttttaattag cacgtaacat ttctaataat tctgtaatat
     5881 ttattaataa aaacagcaag atgtaacgct ttttaacatc aattgattaa atttttggta
     5941 agtgcgattt ttaatttctt atagtaattt ataaataaaa aaatggaaga agcctttcga
     6001 cttctcccct ttaattttct gacaatcttt ttaaattgtc aatctttaaa cttttactcc
     6061 actaccactc tcacctgatt gttgctgcca ttggtttgta cattcagcat gtacgcgcct
     6121 tttgcaacct tgctcaggtc tagttgtaat gtgttttgtc ctgctgcatt ggcatgtgtc
     6181 t
//
LOCUS       DAOOOL010000132         6176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1327_length_6176_cov_53.882536, whole genome shotgun sequence.
ACCESSION   DAOOOL010000132 DAOOOL010000000
VERSION     DAOOOL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_6176_cov_53.882536"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PKU82_09800"
     CDS             complement(<1..1617)
                     /locus_tag="PKU82_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOZ83205.1"
                     /translation="MAYKALNIKLSYEFVRVLPTTDPAYVEYENFKQKFGEDGSVMVI
                     GFADSTIFQIEKFNSWFLLSDKIRKMDGIQNVLSIGDLYDVVRNDSLHKFDFVPLVKT
                     LPQRQSEIDSIKNRISELPFYDGLILNRETHATVMAITFHKKDLDSKHRIDIVNEIKK
                     EADAFGRLHNIELHFSGMPYIRTAVMEKVSHELKLFLILAVLITAFILWIFFRTISSV
                     VFSILVVAIGVVWSMGSIELFNFKITILTALIPPLIMVIGVPNCIFLINKYHSEYARH
                     GNRIKALARMISTVGISLFLANITTAIGFGVLVFTNSSFLTQFGAIAAINVMVTYFIT
                     LIFIPIILSFLPPPSVRQMRHLEGKRMRKILEWVDFIVHHKRNQIYTVIVTVTLLAIY
                     GMTKIDVTGYVVDDLPKKNPVYTDMHFFEKNFKGVLPFEIFVDTKKPNGVFDNNGRVL
                     YKMKALQKQLEKYPELSKALSITEALKFSYQAYRGGDPKYYVLPGISELKNLNDYTST
                     VKGNENRLKSFIDSSRQYTRISMQMADVGSK"
     gene            complement(1742..2551)
                     /locus_tag="PKU82_09805"
     CDS             complement(1742..2551)
                     /locus_tag="PKU82_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83206.1"
                     /translation="MQKIIAVCAAFLISVNCFAGEKDCHCTPLFKHGKGTFYLTWGYN
                     LDWYSKSDLHFKGNDFGGYDFTLYDAKAHDKPGLKYIFGSAISIPQYVYRAGYFFGDK
                     HDLGVEISFDHTKYIMTNNQNLHLKGNINGTFYDKDTLVGKDFLMFEHTNGANFLMAT
                     LMKRYCILSSASKNHRLQLILKAGGGLVIPRTDVTIFGNRLDNKFHIAGYVFGIDAGF
                     RYDLMKYFFVETSLKGAFANFTNVLTVGDGRAHHHFFAGEYIFAAGFQIGL"
     gene            2645..3781
                     /gene="mqnE"
                     /locus_tag="PKU82_09810"
     CDS             2645..3781
                     /gene="mqnE"
                     /locus_tag="PKU82_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOZ83207.1"
                     /translation="MSDIILKSSVDPLLKAIAQKVINEERITPEEGIILYEKGELGFL
                     GSLATLVRNRKNKNFTYFNKNIHIEPTNVCIFSCRFCSYSKDYKHREDGWELTIDEML
                     NKVRAYPSGSITEVHVVGGVHPKMDLHYFAGLLKEIKNINPSLHIKAFTAVELDYMFR
                     KAKMTNAQGLQYLKDHGLDSIPGGGAEIFDEEIRNKICADKCNAEEWLNIHQEAHQLN
                     IPSNATMLYGHYESYSHRVDHMNRLRNLQDKTKGFQTFIPLKFRNKDNEMSHLSETTQ
                     VEDLKNYAVARIFLDNFQHVKAYWPMIGRATAQLSQNFGVDDMDGTIDDTTRIYTMAG
                     SEEQNPTLTTQELVDLISQNGFVAVERDTLYNHLKVYENVPVAN"
     gene            3792..5054
                     /locus_tag="PKU82_09815"
     CDS             3792..5054
                     /locus_tag="PKU82_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOZ83208.1"
                     /translation="MQNNTEPLKNNITIVGAGPAGATASMFLSSFGIKHTLIDKAVFP
                     RDKICGDALSGKVLPVLKKLNPDLIQQLLVQNEKCLGSNGILFGAPNGKTLEVPFKAV
                     NSTLNHSPGYVSKRIDFDFSLIKLLNPEYCNFLQGHEVKKITKGEEGVTLNVLNTQNK
                     ETYNVSSSLVIACDGAHSICNKLFPDHKLDAGHYCGGVRAYYSGVTGFHSENFIELHF
                     LKEMLPGYFWIFPLPNGMANVGAGMLTRTISSKKVNLKEAMLLAIKNNPSIKDRFKDA
                     NIIGNIDGWGLPLGSKKRKLSGNRIILCGDAASMIDPFSGEGISNAMYCGMRAAEVSR
                     DAINSSNYTDDFLSGYDQFVYNRLWNELKLSHTLQKLCNYPSLFNFVVNKASKNETFR
                     QTISCMFDDIDLREKLRKPSFYFKMLFG"
     gene            complement(5119..>6176)
                     /gene="metK"
                     /locus_tag="PKU82_09820"
     CDS             complement(5119..>6176)
                     /gene="metK"
                     /locus_tag="PKU82_09820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOZ83209.1"
                     /translation="KVIKEIGYTKSEYMFEAHSCGVFSAIHEQSTDINQGVERKKPEE
                     QGAGDQGMMFGYACRETDTYMPLAIELAHLLLRELAAIRREGKVMKYLRPDAKSQVTI
                     EYSDDNKPQRIDAIVISTQHDDFAKEAAMQKQIKQDVINILIPRILKKLPKRVQVLFN
                     DKIKYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAA
                     YATRHIAKHLVAAGVCDEVLVQVAYAIGVAQPVGLYVNTYGTSKVKMTDGEISNKLSK
                     MKEFDMRPYFIEKRFNLRSPIYLETAAYGHMGRDSKTVEKVYNKGQANEKKIKVKLFP
                     WEELNAVNETKKVFGIK"
BASE COUNT         2049 a         1140 c         1061 g         1926 t
ORIGIN      
        1 ctttgagcct acatcagcca tctgcatact tatgcgtgta tattgtcttg atgaatcaat
       61 aaaagatttc aatctgtttt catttccttt tactgtagag gtataatcat tcagattttt
      121 aagttcactg atgcctggta atacataata ttttggatca ccacctcgat aagcctgata
      181 gctgaatttt aatgcttcgg ttattgacaa tgctttactt aattcaggat acttttccaa
      241 ttgcttttgt aatgctttca ttttgtatag cacacgtccg ttattatcaa aaacaccatt
      301 cggttttttt gtgtcaacaa aaatttcaaa tggcaataca cctttaaaat ttttttcaaa
      361 gaagtgcata tcggtgtaaa caggattctt ttttggtaaa tcatcaacca catatccagt
      421 gacatcaatt tttgtcatac cgtaaattgc tagaagggta actgtaacaa ttacggtata
      481 aatctgattt cttttatgat gaacaataaa atcaacccat tcaagaatct tacgcatcct
      541 tttcccttca agatgacgca tctgtctaac agaaggtggt ggaagaaaac taagaataat
      601 cggaataaaa attaaggtga tgaaataagt taccattaca ttgatagctg caatagcacc
      661 aaactgagta aggaatgaac tgttagtaaa taccaacaca ccaaaaccaa tggctgtggt
      721 aatgttggct aagaaaagtg agattcccac tgtagaaatc attcgcgcta aggctttaat
      781 acgattacca tgccttgcat attcactatg atatttattg ataagaaaaa tacaattagg
      841 gacaccaata accattataa gtggtggaat caatgcagtt agaatggtga ttttgaaatt
      901 aaataattca atacttccca tactccaaac caccccaata gctactacca atattgaaaa
      961 cacaacggat gaaatggttc gaaaaaaaat ccaaagaata aatgccgtta tcaacactgc
     1021 cagaattaag aataacttca actcatgcga caccttttcc ataacggcag tccgtatgta
     1081 gggcattccg gaaaaatgca attcaatgtt gtgcaatctt ccgaaagcat cagcctcctt
     1141 ttttatctca ttaacaatat caatacgatg tttgctgtca aggtctttct tgtgaaaggt
     1201 tattgccatg acagttgcat gagtttcgcg atttaaaatc aatccatcat aaaatggcag
     1261 ttcgctaatt cggtttttaa tgctgtcaat ttcagactgg cgttgtggca aagttttaac
     1321 taatggaaca aaatcaaact tatgcaacga atcatttctg acaacatcat acaaatcgcc
     1381 aatagaaagc acattttgta tgccatccat ttttctgatt ttatcggaaa gtaaaaacca
     1441 actgttgaat ttttctattt gaaaaatggt actgtctgca aaaccaataa ccataacact
     1501 accatcttct ccgaactttt gcttaaaatt ttcatactca acataagcag ggtcggttgt
     1561 tggtaatacc cgaacgaatt cataagacag tttgatgttt aatgctttat atgccataaa
     1621 cactgtcaac aatagcagta ctactataaa agcaatacgg tttcttaata tcttattcgc
     1681 taaaaatttc cacatgaatg gggcaaagat aagtaaagca acttttatag tcagttaggc
     1741 atcaaaggcc tatttgaaag ccggcagcaa aaatatattc acctgcaaaa aaatgatgat
     1801 gtgcacggcc atcacctaca gttaaaacat ttgtaaagtt agcgaatgca ccttttaaac
     1861 ttgtttcaac aaaaaaatat ttcattaaat catagcgaaa tcccgcatca ataccaaaca
     1921 catagcctgc aatatgaaat ttattatcta atctgttacc aaatatggtt acatctgttc
     1981 gtgggattac caaaccacca ccggctttca atatcaattg caatctatga ttttttgaag
     2041 ccgatgaaag aatacaatag cgcttcatca gagtagccat caaaaagttg gcaccattcg
     2101 tatgctcaaa catcaggaag tctttgccaa ccaaagtatc tttatcataa aacgtaccat
     2161 tgatgttgcc tttaagatgt aaattctgat tgttggtcat aatatatttg gtgtgatcaa
     2221 atgaaatctc tacacctaaa tcatgtttat caccaaaaaa ataaccggca cgataaacat
     2281 attgagggat agaaatggct gaaccaaaga tatatttcaa ccccggctta tcgtgtgcct
     2341 tcgcatcata taatgtgaaa tcataaccac caaaatcatt acctttaaaa tgcagatcgc
     2401 tctttgaata ccaatcgagg ttgtagcccc atgtcagata aaatgttcct ttgccatgtt
     2461 taaataaagg cgtgcaatga caatcttttt caccggcaaa acaatttaca gaaatcaaga
     2521 atgccgcaca aacagcaata atcttttgca tattaatatc aaataaatgg cgcaaagcta
     2581 catgaaaatc aaaaccattt tcttaatttt atgtttcttt gcataatatt tggaattgag
     2641 aatgatgagt gatataattt taaaatcatc tgttgacccg ttgttgaaag caattgcaca
     2701 gaaagttatt aatgaagaga gaattacacc tgaagaagga ataatacttt acgaaaaagg
     2761 cgaacttggt tttttaggaa gcttagcaac cttagtaaga aaccgcaaaa acaaaaattt
     2821 cacttatttt aataagaata ttcatattga gccaacaaac gtttgtattt tttcttgcag
     2881 attttgcagt tatagcaagg actacaaaca ccgcgaagat ggctgggaac ttactattga
     2941 tgaaatgtta aataaagtga gggcatatcc ttcaggaagt attaccgaag tgcatgttgt
     3001 tggtggtgta catccaaaaa tggatttaca ttattttgcc ggtttgctaa aagaaattaa
     3061 aaacatcaac ccttctttac atataaaagc atttactgct gttgaattgg actatatgtt
     3121 tcgcaaagca aaaatgacaa atgcacaagg acttcaatat ttgaaagatc atggattaga
     3181 tagtattccc ggtggtggtg cagaaatttt tgatgaggaa attcgcaata aaatctgtgc
     3241 agataagtgt aatgctgagg agtggttgaa tattcatcag gaagcacatc aattaaacat
     3301 accctcaaat gcaacaatgt tgtatggtca ttatgaaagc tattctcatc gtgttgacca
     3361 catgaacaga ttaagaaatc tgcaggataa gactaagggc tttcagacat ttattccctt
     3421 aaaattcagg aataaggata atgagatgag ccatctttct gaaactactc aggtagaaga
     3481 tttaaaaaat tatgctgttg cccggatttt tttagataac tttcagcatg taaaagctta
     3541 ttggcccatg ataggcagag ccactgcaca actctcacaa aattttggtg ttgacgatat
     3601 ggatggcact attgatgaca caacacgcat ttatacaatg gcaggttctg aggaacaaaa
     3661 tccaacttta accacgcaag aattagttga tttgatttcg cagaatggtt ttgttgccgt
     3721 tgaacgcgat acactgtaca atcatttgaa agtttatgaa aatgttccag tagctaattg
     3781 aaagatttat tgtgcagaat aatactgagc cattaaaaaa caacataact atagtaggtg
     3841 caggcccagc aggtgcaacg gcatctatgt ttctatcttc ctttggaatc aaacatactt
     3901 tgattgataa agctgttttt ccgcgcgaca aaatttgtgg tgatgcatta agcggaaaag
     3961 ttctacctgt attgaagaaa cttaatcccg atttaattca gcagctatta gtacaaaacg
     4021 aaaaatgtct gggaagcaat ggaattttat ttggtgcacc caatggcaaa acattagaag
     4081 taccttttaa agccgtaaac tcaactttaa atcattcacc cggatatgtg agtaaacgaa
     4141 ttgatttcga tttttcatta ataaaattat tgaacccgga gtattgtaat ttcctgcaag
     4201 gccatgaagt aaagaaaata acaaaaggcg aagaaggtgt cacactgaat gttttaaata
     4261 cacagaataa agaaacttac aatgtaagtt catctttagt cattgcctgt gatggtgcac
     4321 atagcatttg caataaatta tttcctgatc acaaactcga tgcaggacac tattgcggag
     4381 gcgttcgggc atactattcc ggtgtgacag ggtttcatag tgagaatttt attgagttac
     4441 attttctgaa agaaatgcta cccggatact tctggatatt tccattaccc aatggcatgg
     4501 ctaatgttgg tgcaggtatg ctgacacgaa ccatcagttc aaaaaaggta aatctgaaag
     4561 aagctatgct tcttgccatt aaaaataatc cttccataaa agatcgtttt aaagatgcaa
     4621 acattatagg caacattgat ggatggggat tgccattagg ttctaagaaa agaaaattat
     4681 caggcaacag aattatttta tgtggtgatg cagcaagcat gattgatcca ttcagtggtg
     4741 agggtatcag caatgccatg tactgcggca tgcgtgccgc agaagtgtcg cgtgatgcaa
     4801 taaatagcag caactatacc gatgattttt tatcaggtta tgatcagttt gtttataaca
     4861 gactttggaa tgaattaaaa ttaagtcata cacttcaaaa actttgcaat tatccttcgc
     4921 tgtttaattt tgtagtcaat aaggcgtcaa aaaacgaaac cttccgtcag accataagct
     4981 gtatgtttga tgatatagat ttaagagaga aactacgcaa gccatccttt tattttaaaa
     5041 tgctttttgg ctgatagcaa caatttagtt tctattgcaa atactcttca ataaaaaagc
     5101 cccttgtggg gctctttctt atttgattcc aaaaactttc ttcgtttcgt taactgcatt
     5161 taattcttcc caaggaaaaa gttttacttt aatcttcttc tcgttcgctt gtcctttatt
     5221 gtaaaccttt tcaaccgttt tagaatcgcg acccatgtgt ccataggctg cagtttctaa
     5281 ataaattggt gaacgaagat taaaacgttt ctctataaaa tatggacgca tatcaaactc
     5341 tttcatcttt gaaagtttgt ttgatatttc tccgtcagtc atttttacct ttgatgtgcc
     5401 ataggtattt acatacaaac caacaggctg tgcaacgcca atagcataag caacctgaac
     5461 aagaacttcg tcacatacac cggcagctac aaggtgtttt gcaatatgtc gtgttgcata
     5521 agccgcacta cggtcaactt ttgaaggatc ttttccactg aaagcaccac cgccatgtgc
     5581 gccttttcca ccataagtat caacaatgat ttttcttcct gtaagtccgg tatcaccatg
     5641 tggcccgcca atcacaaatt ttcctgttgg gttaacgtga tatttaattt tatcattaaa
     5701 caacacctga actctcttag gaagtttttt aagaatacgc ggaattaaaa tattgataac
     5761 atcttgttta atttgttttt gcatggctgc ctcttttgca aagtcgtcat gctgtgtaga
     5821 aataacaatt gcatcaattc tttgtggctt gttatcatca ctatactcaa tagttacctg
     5881 cgattttgca tccggacgaa gatatttcat cacttttcct tctctgcgaa ttgcagctag
     5941 ttcacgaagt aataagtgtg ccaattcaat tgctaaaggc atgtaggtat ctgtctcgcg
     6001 gcaggcatat ccaaacatca ttccctgatc accggcacct tgttcttcgg gctttttgcg
     6061 ttctacaccc tgattgatgt ctgtgctttg ttcgtgaata gcagaaaaca caccgcaaga
     6121 atgggcttca aacatatact cactctttgt ataacctatt tctttgataa ccttac
//
LOCUS       DAOOOL010000133         6163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1331_length_6163_cov_111.606090, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000133 DAOOOL010000000
VERSION     DAOOOL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1331_length_6163_cov_111.606090"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..727
                     /gene="paaC"
                     /locus_tag="PKU82_09825"
     CDS             2..727
                     /gene="paaC"
                     /locus_tag="PKU82_09825"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HOZ83210.1"
                     /translation="MRLADSNMIHGQRLAEWCSRGPFLEEDIALSNMSLDMFGQAEML
                     YEYASSLSEDELSADDIAFRRSEREYFNCLLVEQENGDFAQTMMKVFLYSAFAKQLYE
                     SLLNSNDETLSGFAAKAIKEVKYHLRHSSEWLIRFGNGTDESKMRVQQAVTNLWRFTQ
                     DMFEMNDADTALIDNGISFTTDTLFAKWQLNINEIFAAANINTPEVNNTITGGHNGIH
                     TERLGVMLCEMQYLQRAYPDAKW"
     gene            739..1248
                     /gene="paaJ"
                     /locus_tag="PKU82_09830"
     CDS             739..1248
                     /gene="paaJ"
                     /locus_tag="PKU82_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOZ83211.1"
                     /translation="MSSVKNTESSKSTESLIWALLHTVPDPDIPVISIVDLGIVRNVE
                     WKNEKAIITITPSYSGCPAMSVFVDDIKQVLNGAGFQSEIKTILSPAWTTEWISQSGL
                     DKMKAHGIAPPEDETGMDSIFAMQKPVTCPRCNSKQTKMLNRFGSTPCKALWYCNECS
                     QPFEYFKCH"
     gene            1248..1703
                     /locus_tag="PKU82_09835"
     CDS             1248..1703
                     /locus_tag="PKU82_09835"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOZ83212.1"
                     /translation="MMNETVRVTKIFVFDMAHALFGYDGPCKNIHGHTYKLSVTLKGK
                     PFNDNSNPKAGMVIDFSILKQIVQEKIIKQFDHALVLNNDSPHKLLDKVLTQQFEKII
                     YLDVQPTCENLLLHFKKQLASHFADKIKLVYLKLEETPTSYSEWCIDDN"
     gene            complement(1753..2142)
                     /locus_tag="PKU82_09840"
     CDS             complement(1753..2142)
                     /locus_tag="PKU82_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ83213.1"
                     /translation="MITTPLKIFIVDDDNMMLEMLRDRIGEHTPHTVLTFGTGEDCLK
                     HVAENPDVIILDYYLNSKNPDASNGMEILKAIKKYNPNIHVIFLSGQERYGVAMQTLQ
                     KGAEYYVIKDNEAFGKIEKILSGITVS"
     gene            complement(2195..3409)
                     /locus_tag="PKU82_09845"
     CDS             complement(2195..3409)
                     /locus_tag="PKU82_09845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ83214.1"
                     /translation="MLTFAQLMNTVVAIIFWISLLGLLHSYVLFPLLMRLLLMLKHKS
                     KDDDDNNLFKPMVSVVMAAYNEEKVIIQKLESVIKSNYPSDKLEIIVGSDCSNDSTDM
                     LVKEFAHNHPQVKFIRFNSRTGKPAIINQLVKQCKGEFLIITDANVMFDAEAISSMMH
                     RFTVAEVGLVDSNMVNIGLMKSGISSQEKQYIQTEVLTKYAEGEVWGSMMGPFGGCYA
                     IRKSLFSDIPSNFLVDDFYVCMKVLEQGYHSVSSLKAIVYEEASHNLWIEFRRKTRIA
                     TGNFQNLAKFSKMIFKGRGIGFCFMSHKVLRWKGPVLMLLLYFSSIAAWWLSIDINPV
                     LYLGVFAAISLSLLLLLADRLLNLFQIHIGLLRLNTHFLVMNAAMLKGMFRYIKGVKS
                     GVWQPTSRTQNA"
     gene            3457..4371
                     /locus_tag="PKU82_09850"
     CDS             3457..4371
                     /locus_tag="PKU82_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83215.1"
                     /translation="MNKKRTFAALIFVYMRFIISFVILLTHYISGYSQERRGPFVKTD
                     LFTLDRDRVIDGGIFTYRIAVDADEIIAFNSCNQPYVFLINNQGTILEEIKLPYEGCL
                     RNMEFDEFDNLMMMDNEETRIFKYVRKTKKIEVIPYNKPEDWYKSLNHFYRFFEIASI
                     PTYYNNPEYYQDFYKTRFHYSYNLWLNYQDGYIYQFAYNFIKKVGNRKTYLALKRSDL
                     WFSDRLTNKCKPLLVDLEKETAVYFDRSLTLYFEDFKNHTLQTWPLAEGHDEAVQLDY
                     ATNIKQKKVWGVSTFDNKHLTIAVWEIK"
     gene            complement(4378..5352)
                     /locus_tag="PKU82_09855"
     CDS             complement(4378..5352)
                     /locus_tag="PKU82_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOZ83216.1"
                     /translation="MESSKIKIKSVLQFFLKATIALFSLWFIVHKIIHRENEVSFFDF
                     VNTRLHTEGAYSLLISVIVLMVLNWSIEAIKWKVLINRLTKISFVRSLSAVFAGATVS
                     FFTPNRVGDYAGRMLFLPSSVRIASLLSTFAGNISQLIVTLCCGLAAMALSLQHYVQW
                     NESLVVTTRVASFVFALILFVSFPYIRIVFKMPLLNFVRQRFPEYVMHLENYSYREMM
                     HILFLSLLRYSVFAFQYILLFLVSGIPVIPSMIVIIALIFFIQALVPTLALTEFAIRG
                     SVALMVAAPFIQNDQAVLMASFALWLINLAVPAAIGSVMIFYLNFRKE"
     gene            5403..5969
                     /gene="ruvC"
                     /locus_tag="PKU82_09860"
     CDS             5403..5969
                     /gene="ruvC"
                     /locus_tag="PKU82_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOZ83217.1"
                     /translation="MSESEKIILGIDPGTNVMGYGVLLISGTEMKLETAGIIHLEKFD
                     NHPLKLKHIFEKTLRLIESYLPDEMAIEAPFFGKNVQSMLKLGRAQGVALAAALFKNI
                     PVFEYSPKKIKQSVTGNGSATKEQVAAMLQQLLHFEEMPKYLDATDGLAAAVCHHFQK
                     NTGQSQFAGGKSKGWQKFISDNPDKLIG"
BASE COUNT         2102 a         1152 c         1090 g         1819 t
ORIGIN      
        1 cctgcgatta gccgacagca atatgattca tggccaacgt ttggctgaat ggtgcagcag
       61 aggacctttt ttagaagaag atattgcttt atcaaatatg tcattagaca tgtttggtca
      121 ggctgaaatg ctttatgaat atgcatcatc attatcggaa gatgaactta gtgctgacga
      181 tattgctttc agacgcagtg aaagagaata tttcaactgc cttttggtag aacaggaaaa
      241 tggcgacttt gcacaaacga tgatgaaagt gtttttgtat tctgcatttg caaaacaact
      301 ttatgaatcg ttgttgaaca gcaatgatga aacattatct ggttttgctg ctaaagctat
      361 taaggaagta aaatatcatt tgcgacacag cagcgaatgg ctcatcagat tcggaaacgg
      421 aactgatgaa agtaaaatgc gtgtgcagca agctgtcaca aatttgtggc ggtttacaca
      481 agatatgttt gaaatgaatg atgcagacac tgcattaatt gataacggta tctcttttac
      541 aacggatacg ctatttgcga agtggcaact aaacataaat gaaatattcg ctgcggcaaa
      601 tattaacact ccggaagtaa acaacaccat aacaggtggc cacaatggta ttcatacaga
      661 acgcttaggt gtcatgcttt gcgaaatgca atatttgcag agagcctatc ctgatgctaa
      721 gtggtagaat aaaaagtcat gtcatcagta aaaaatactg aaagcagcaa gtcaacagaa
      781 tcattaatct gggcattatt gcatactgtt cctgatcctg atattcctgt tatcagcatt
      841 gttgatttag gcattgtccg caatgttgaa tggaaaaacg aaaaagcaat tatcacaatt
      901 accccaagtt attcgggctg tcctgccatg tctgtttttg tggacgacat caagcaggta
      961 ttaaatggtg cagggtttca gtcagaaata aaaactatac tatcacccgc ctggactaca
     1021 gaatggataa gtcagtcagg acttgacaag atgaaagccc atggaattgc accccctgaa
     1081 gatgaaaccg gaatggatag tatatttgca atgcaaaaac cggttacctg cccccgttgc
     1141 aacagcaaac aaactaaaat gctgaatcgc tttggctcaa caccatgcaa agcactttgg
     1201 tattgcaacg aatgtagtca gccgtttgag tattttaaat gtcattaatg atgaatgaaa
     1261 ctgttcgggt aactaaaatt tttgtgtttg acatggctca tgccttattt ggctacgatg
     1321 gtccatgtaa aaatattcat ggtcacacct ataaactttc tgtcacctta aagggaaaac
     1381 cttttaatga caatagcaat ccgaaggctg gtatggtcat tgatttttca atattgaaac
     1441 aaatagtaca ggaaaaaatt atcaaacaat ttgatcatgc cttagtactc aacaacgatt
     1501 cgccacataa gctgcttgac aaagtactta cacaacagtt tgaaaaaatt atttatttag
     1561 atgtgcagcc aacctgcgaa aatctgttgt tgcattttaa gaagcaactt gcatctcact
     1621 ttgctgataa aataaaattg gtttatttaa aattagaaga aactcctact tcttattctg
     1681 agtggtgtat tgacgacaac tgataactaa gcggtgaata aaaaatttca gtatcaactt
     1741 tttagagttg atttatgaaa ctgtaatgcc gcttaatatt ttttcaattt taccaaaagc
     1801 ttcattgtct ttaatgacat aatactctgc tcctttttgt aaggtttgca tagccacacc
     1861 atagcgttcc tgtcccgaaa gaaaaattac atgaatgttt ggattgtatt tcttgatagc
     1921 tttcaatatt tccataccat tagatgcatc aggatttttt gaattcagat aataatcaag
     1981 aataataaca tcgggattct ctgctacatg cttcaaacaa tcttcgcctg tgccgaaagt
     2041 taaaaccgta tgtggtgtat gctcaccaat tctgtcacga agcatttcca gcatcatatt
     2101 atcatcatca acaataaata tttttaaagg tgttgttatc attttgttta gtgtcaaatg
     2161 gaatacaaac ctacaatttt tttgtaatat ccaattaagc attctgagtc cggcttgtag
     2221 gttgccaaac accgctttta actcctttaa tgtatctgaa catacccttc aacatagcag
     2281 cattcatgac taagaaatgt gtatttaatc tgagcaatcc tatatgtatt tgaaacaaat
     2341 taagtagcct gtctgccagc aacaaaagta aacttaatga tatggctgca aaaactccca
     2401 gatacaaaac aggatttata tcaatagaca accaccaggc cgcaatagaa gagaaatata
     2461 aaagcaacat caacacaggg cctttccatc tcaaaacttt atgagacatg aaacaaaatc
     2521 caatacctct gcccttaaaa atcatcttgc taaactttgc cagattctga aaatttcctg
     2581 tggctatacg cgtttttctc ctgaactcta tccaaagatt atgcgatgcc tcctcataga
     2641 caatagcttt tagggaactt acagaatgat aaccctgctc cagtactttc atgcagacat
     2701 agaaatcatc aaccagaaag tttgaaggaa tatcgctgaa taaacttttc cttattgcat
     2761 aacacccacc gaaagggccc atcatacttc cccatacttc accttcggca tatttggtta
     2821 aaacttctgt ttgaatgtac tgtttctcct gagatgaaat tccgcttttc atcaatccaa
     2881 tattcaccat gttgctatca actaagccaa cttctgctac agtaaatcta tgcatcatag
     2941 atgaaattgc ttcagcatca aacattacat tggcatcagt aataattaaa aactcaccct
     3001 tacactgttt taccaattga ttgatgattg caggttttcc tgttctgctg ttgaatcgaa
     3061 taaactttac ttgtggatga ttatgtgcaa actctttcac caacatatca gtactatcat
     3121 tggaacaatc agagcctaca attatttcta atttatctga tgggtaatta cttttaataa
     3181 cactttcgag cttttgaatg atgaccttct cttcattgta agcagccatc acaacgctga
     3241 ccatcggctt aaataaatta ttgtcatcat catctttaga cttgtgcttt aacattaaaa
     3301 gtaaacgcat gagtaatgga aacagtacat aagaatgtaa aagtcccaaa agcgaaatcc
     3361 agaaaataat ggcaacaaca gtattcatca attgtgcaaa ggtcaacatt ttaacttcaa
     3421 atagagaaga ttttacctgt ctttttgtgg ctttatatga ataaaaagag gacttttgca
     3481 gccttaattt ttgtttacat gcggtttatt atttctttcg ttattcttct tacacattac
     3541 atttcaggtt atagtcagga aagacgtggc ccctttgtta agacagacct ttttacactt
     3601 gaccgcgaca gagtgattga cggtggaatt ttcacataca gaattgcagt agatgccgat
     3661 gagataattg cattcaattc ttgtaaccag ccttatgttt ttttaattaa caatcaggga
     3721 accattcttg aagaaattaa acttccttac gaaggttgtt tgcgcaacat ggagtttgat
     3781 gagtttgaca acctgatgat gatggacaat gaagaaacac gaatcttcaa atatgttcgt
     3841 aagacaaaaa aaatagaagt cattccttat aacaaacccg aagactggta taaaagtctg
     3901 aatcattttt atcgtttttt tgaaattgct tcaattccta cttactataa caatcccgaa
     3961 tactatcagg atttttataa aacacgcttt cattactcat acaacctgtg gttaaactat
     4021 caggatggct acatctatca gtttgcctat aattttatca aaaaagtcgg taacagaaaa
     4081 acctatcttg cattaaagcg tagcgacctt tggttcagcg acagacttac aaataaatgc
     4141 aaaccgcttt tagttgatct ggaaaaagaa acggcagttt attttgatcg ttcactgact
     4201 ttatattttg aagattttaa aaaccacact ttacagacat ggcctttggc agaaggacat
     4261 gatgaggcag tgcagttgga ctatgctacc aatatcaaac aaaagaaagt ttggggtgta
     4321 agtacttttg ataataaaca tcttactatc gctgtttggg aaatcaaata atgagcgtta
     4381 ttcttttctg aaatttaaat aaaaaatcat taccgaccca attgctgcag gcactgccag
     4441 attaatcagc cagagtgcaa atgatgccat tagaactgcc tgatcattct gaataaaggg
     4501 tgctgcaacc atcaaagcaa cagaacctct tattgcaaac tctgtcagtg caagtgttgg
     4561 aaccaatgcc tgaataaaaa agatgagtgc tataatgaca atcatagatg gaataaccgg
     4621 aatgccggaa accagaaata gcaatatgta ctgaaaagca aaaacagaat agcgcagcag
     4681 agaaagaaaa agtatgtgca tcatttctct gtaagaataa ttttcaagat gcatgacata
     4741 ttcaggaaat cgctgtcgaa caaaattcag caaaggcatt ttaaaaacaa ttcttatata
     4801 aggaaaagaa acaaaaagta tcaatgcaaa aacaaatgat gcaacacgtg ttgtgacgac
     4861 taaactttca ttccactgta cataatgttg taatgacaaa gccattgcag caagtccaca
     4921 acaaagtgta acaatgagct gactgatatt tcctgcaaaa gttgaaagca gtgatgctat
     4981 gcgaacagat gaaggcaaaa aaagcattct acctgcatag tcgccaacac ggttaggtgt
     5041 aaaaaagcta acagtagccc cggcaaaaac cgcacttagg cttcgaacaa aactaatttt
     5101 ggtgagtcta tttattaaga ccttccactt tatagcttct atactccaat tcaacaccat
     5161 caatacaata acagatatga gcaaggaata agcaccttct gtatgcaggc gagtattcac
     5221 aaaatcgaaa aacgacactt cattctcgcg gtgtataatt ttatgaacaa tgaaccaaag
     5281 agaaaacaat gcaattgttg ctttcagaaa aaactgtagc acacttttaa tctttatttt
     5341 tgaactttcc aagcaattgc agattatagt gcaagtttaa aaaataagcg gtaatagcgt
     5401 tcatgtcaga aagcgaaaaa ataattttag gaatagatcc gggcactaat gtaatgggat
     5461 atggagtact ccttatttcg ggaacagaaa tgaaattaga gaccgcaggt ataattcatc
     5521 tcgaaaaatt tgacaaccat ccactgaaat taaagcatat ttttgagaaa acattacgcc
     5581 ttattgaaag ctatttgcct gatgaaatgg caattgaagc accgttcttt ggcaagaatg
     5641 tacaatctat gttaaagtta ggtagggcac aaggagttgc acttgccgct gcattattca
     5701 aaaatattcc tgtatttgaa tacagcccaa aaaaaataaa acaatctgtc accggcaatg
     5761 gtagtgccac caaggagcag gttgctgcta tgcttcaaca gttgttacac tttgaagaaa
     5821 tgccaaaata tcttgatgct accgatggcc tggcagctgc agtatgtcat cactttcaga
     5881 aaaataccgg gcaaagtcag tttgcgggag gcaaaagcaa aggatggcaa aaatttattt
     5941 ctgataatcc cgacaaatta atcgggtaga gaaactaagt ttatcctgac tatctgaaca
     6001 atggaaatgc ctgtggattt acagagtgca ttaattcata cacagtatta aacacatctt
     6061 ccgcattagg cttcgaaaaa taatctccat cgcttccgta tgccggacga tgttcttttg
     6121 cagcaagagt tacaggcggt gtttcaagaa actcaaatgc ttt
//
LOCUS       DAOOOL010000134         6157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1336_length_6157_cov_118.847591, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000134 DAOOOL010000000
VERSION     DAOOOL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1336_length_6157_cov_118.847591"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1173
                     /gene="thiL"
                     /locus_tag="PKU82_09865"
     CDS             121..1173
                     /gene="thiL"
                     /locus_tag="PKU82_09865"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOZ83218.1"
                     /translation="MFENANNRTELSSLGEFGLIRHLENAVEIKNKETLKGIGDDAAV
                     ICLDSEENVQLITTDMLLEGVHFDLVYCPLKHLGYKSVVVNLSDIYAMNGIPKQIVVG
                     IGLSNRFSLEAIEELYSGMLLACKHYGVDLIGGDTTSSRSGLVISITAIGTAKKSDVV
                     YRNGAQENDLICVSGDLGAAYLGLQLLNREKKVFLDSPGVQPDFQGNDYLLERQLKPE
                     ARKEIIEILKKTGVKPTAMIDISDGLASEIMHICTQSDKGCTIYEAKIPIDEKTYNLA
                     REFHLDPTVCAMNGGEDYELLFTINQKDFEKINSEADISIIGHITAKEEGYYMLDKSD
                     RKVALKAQGWDALKSN"
     gene            1191..1970
                     /locus_tag="PKU82_09870"
     CDS             1191..1970
                     /locus_tag="PKU82_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOZ83219.1"
                     /translation="MSNKNYRIICWNVNGIRAIWKKGFPEWLSKEMPDVLCLQETKAQ
                     PEQLDDEIKDFNGYKSHFFSAQKKGYSGVAIYTKREPLKVRYGLDDDAFDIEGRCIEM
                     EFEDFVLYTAYFPNGGRGPERVKYKLDFYESLFQRAENMRKKNKNIVICGDYNTAHQE
                     IDLARPKENSKTTGFLPIEREWIDKIIGMGYVDIFREFNQESGQYTYWDQITRARDRN
                     VGWRIDYYMISTELRKYAKSAPIHMNVMGSDHCPIELNLSF"
     gene            complement(1999..2982)
                     /locus_tag="PKU82_09875"
     CDS             complement(1999..2982)
                     /locus_tag="PKU82_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOZ83220.1"
                     /translation="MQHDPYVILGISPSATPEEIKRAYRKKAFELHPDRNSDQHAEQL
                     FRELNEAYALLTDADKRRNYDKQYQNYSRSNTDQFDHYFEMHVSSNNVKVCEEFELIF
                     TYTGEGRFMQKPDLRDFYITGKPFVSFRDVYRSGKTARETTIRYVLAAKRVGSFTINR
                     AAIKLFGKKFITATQYIQVISGECFFSPDKPADGRPLQVSLWYNAEKGGTNRKYLVNT
                     KHTIYIPRSNYAQVYHTIGRVLKLIFAFWGFFVAVKISRSGIIGFAIGSAYGAIMTYL
                     LYYFVKVKPVFMSERKYSLVISYLQNGYQPYPPHARNLISRALMFINKLFL"
     gene            3075..4931
                     /locus_tag="PKU82_09880"
     CDS             3075..4931
                     /locus_tag="PKU82_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ83221.1"
                     /translation="MKNYFKILRYVKPYWGYALLNASCNILSVLFSFFSLTLIAPFLD
                     LLFLKDDNFYLEKMAAGKPEFHLSTKAIVDSFYYYLTEMITDPNRGKQYALMFICIAV
                     AVMFFLKNLFRYLGQFFISPIRNGVVKDLRADVHKKMMRLPLSWFSNERKGDIITRVT
                     SDVHEVEWSVMSSLEVVFRDPFNIFLFLGMMIFISAKLTVFVFILLPIVGFLIGRIGK
                     SLKRTSARSKEKLGLLISIIEETLSGLRIIKAFNAEKFMAEKFAKVNERYNTIAIRAL
                     RKTDLSSPLSEFLGTVTMMIVMYFGGMLVLGKDASLSASAFITYIALFSQIIPPAKSF
                     TQAFYNIQKGLASADRIFAVLEAEESIKDQQSAETIKSFNYKIEYQNISFSYRKGDEG
                     YVLKNVSFTIEKGKTVALVGQSGSGKTTLADLLPRYYDVDGGTLLIDGTDIRKLQLHS
                     LRELLGVVSQEPILFNDTIYNNIAFGMNHATEKMVEEAASVANAHEFITQMPEGYQTN
                     IGDRGSKLSGGQRQRISIARAVLKNPPILILDEATSALDTESEKLVQDALTSLMKNRT
                     VLVIAHRLSTIQHADEIIVLQKGEIVQRGTHVQLANVEGVYKKLYELQGLSS"
     gene            complement(4941..5702)
                     /locus_tag="PKU82_09885"
     CDS             complement(4941..5702)
                     /locus_tag="PKU82_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ83222.1"
                     /translation="MKIVIFEDEEPASRRLIKLLNEINANVEIVSVIDSVRSGKEFMS
                     QNKNFDLIISDIRLADGLSFEIFKSYPVKLPVIFSTAYDEYAIEAFRNNGIDYLLKPV
                     KREELKSAIQKYENWFSDSTSGVNYDALLQTLLQEKKGFQKRIVIRFADTIKTVEIED
                     AAYFFTENKINNLCTFGGESFIVDHNLDELELMLDPSVFFRINRQFIVNFKAIQKMNV
                     VSKSRVKLTLHPTTIQDTIVSTERSGVFKQWLAGK"
     gene            complement(5702..>6157)
                     /locus_tag="PKU82_09890"
     CDS             complement(5702..>6157)
                     /locus_tag="PKU82_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ83223.1"
                     /translation="VDYVENLSAFFRNIVTYKDKETIKLSEEIKIAKAFYFIQQKRFG
                     SNLQLLIDEHSLYDEKYVVPMVLQLLLENAIKHNEISKEKNLEVHVAFTNDRIIISNN
                     TNPKVSKDESTGTGLRNIIARYRLLTDKTVQIESSQDYFVVSLPLINHQ"
BASE COUNT         1965 a         1054 c         1168 g         1970 t
ORIGIN      
        1 gtggtgcaaa gataggttta attgctgtta tagtcaacaa ttacgaggta taaaagttta
       61 tcaaaggcta ttcattacca gatttttact tttgtcatct tcaataaaga ataataaatc
      121 atgtttgaaa acgcaaacaa ccgcacagag ttgagtagtt taggtgagtt cggacttatc
      181 aggcatctag aaaatgctgt tgaaatcaag aataaagaga cactaaaagg cattggtgat
      241 gatgctgctg ttatttgttt agatagcgaa gaaaatgttc agcttattac tactgatatg
      301 ctgctcgaag gtgtacactt tgaccttgtt tactgtccgt taaaacattt gggttataaa
      361 tcggtggtgg taaacctgtc cgacatttac gccatgaatg gaatacccaa acaaattgtt
      421 gtgggaatag gtttgagtaa ccgcttttcg ctcgaagcca ttgaagaact ttacagcgga
      481 atgttgcttg catgcaaaca ttatggtgtt gatttgattg gtggcgatac taccagcagc
      541 cgcagtggtt tagtgatttc aatcactgcc attggtacag caaaaaaatc tgatgtggtt
      601 tacagaaatg gtgctcagga aaatgatttg atttgtgtga gtggtgattt gggtgctgca
      661 tacctaggct tgcaactact caacagagaa aagaaagttt ttttagacag tcccggagtg
      721 cagcccgatt ttcagggtaa cgactacttg cttgagcgac agttaaagcc cgaggcacgg
      781 aaagaaatta ttgaaatatt gaaaaaaaca ggtgtaaaac ctacagccat gattgacatc
      841 agcgatggat tagcttctga aattatgcac atctgcacgc agtcagacaa aggttgcaca
      901 atctatgaag ctaaaattcc tattgatgaa aagacttata atctggcacg tgagtttcat
      961 ttagacccaa cagtgtgtgc catgaacggt ggtgaagact acgaactgtt atttactatc
     1021 aaccaaaaag attttgaaaa aattaacagc gaagctgaca tatctattat cggtcacatc
     1081 actgctaagg aagaaggtta ttacatgctc gataaatctg accgtaaagt ggcattgaaa
     1141 gcacaaggct gggatgcatt aaaatcaaat taaaccttta tcactaaaaa atgagtaaca
     1201 aaaattatcg cattatttgt tggaatgtaa acggcataag agctatctgg aaaaagggat
     1261 ttccggaatg gttgtcaaaa gaaatgcctg atgtattatg tcttcaggaa accaaggcac
     1321 aacctgagca attggatgat gaaataaaag atttcaatgg ctacaaaagt catttttttt
     1381 ctgcacagaa aaaaggatac agcggtgttg ccatttacac caaacgtgag ccattgaaag
     1441 taaggtatgg attagatgat gatgcttttg atatagaagg ccgttgtatt gaaatggagt
     1501 ttgaagattt tgtgttatac actgcatatt tcccaaatgg tggtcgtggt cctgaacgtg
     1561 tcaagtataa actggatttt tacgaatcac tttttcagag agctgaaaac atgagaaaga
     1621 aaaataaaaa cattgtcatt tgcggagact ataatactgc acatcaggaa atagatttgg
     1681 cacggccgaa agaaaattca aagacaacag gatttttgcc tattgaacgc gagtggattg
     1741 acaagattat cggcatgggg tatgttgaca ttttcaggga gtttaatcag gaatccgggc
     1801 aatacaccta ctgggatcag attacacgtg cccgcgacag aaatgtaggc tggcgcatag
     1861 attattatat gatttcaact gaactgcgca aatacgcaaa gagtgctccc atacacatga
     1921 atgtaatggg cagtgatcat tgtccaatag aactgaattt gtcattctaa atctccgttc
     1981 aaaatttttt atctcacttt acaaaaacag tttgttgata aacatcaatg cacggctaat
     2041 caagttacgc gcatgaggag gataaggctg gtagccattt tgaagatagg agattaccaa
     2101 tgaatatttt ctttctgaca taaaaacggg cttcaccttt acgaagtagt aaagcagata
     2161 ggtcataatt gctccataag cagagccaat ggcgaatcct ataataccac ttctgctaat
     2221 cttaactgca acaaaaaatc cccaaaacgc aaaaattaat ttaagcactc tgccaatagt
     2281 atggtaaacc tgtgcataat tacttctggg aatataaatg gtgtgtttgg tgttgactaa
     2341 atattttctg tttgttcctc ctttctcggc attataccat agtgaaacct gcaaaggtct
     2401 tccatcagca ggcttatcag gagaaaaaaa acattcaccg gaaataacct gaatatactg
     2461 agttgcagta ataaactttt taccaaataa cttaatagca gcacgattta ttgtaaaact
     2521 acctactctt ttcgctgcaa gcacatagcg aatggttgtt tcccttgcag ttttacccga
     2581 acgatataca tcgcgaaatg atacaaatgg tttaccggtt atataaaaat cacgcaaatc
     2641 tggtttttgc ataaatcgtc cttcaccggt ataggtaaaa atcaactcaa actcttcgca
     2701 cacttttaca ttattactgc tgacatgcat ttcgaaataa tggtcaaact gatcggtatt
     2761 gctgcgtgaa tagttttgat attgcttatc ataatttctt cttttatcag catcagtcag
     2821 taaggcataa gcctcattca actccctgaa tagttgttca gcatgttggt cgctgttacg
     2881 gtccggatgt agttcgaaag cttttttacg ataagcccgc ttaatctctt cgggagtggc
     2941 cgaaggtgat atacctaaaa ttacatacgg atcatgttgc atttagcgtc tgtgggtaac
     3001 aattaaacaa tattgaacat ttttgtgtta gtttgcaact gttttcagga ttttatttac
     3061 aagaactatt ttttttgaaa aattacttta aaatacttcg ttacgtaaaa ccttattggg
     3121 gctatgcatt attgaatgca tcgtgcaata tcttatcagt tctgttttca tttttttcat
     3181 taacattaat tgctcctttt cttgatttat tgtttctgaa agatgataac ttttatttag
     3241 agaaaatggc tgccggcaag ccggagtttc atttgtcaac aaaggctatt gttgatagtt
     3301 tctattatta tcttactgaa atgatcactg acccaaacag gggtaagcaa tatgcattaa
     3361 tgtttatttg tattgcagtg gcagtgatgt tttttcttaa aaaccttttt cgctacttag
     3421 ggcaattttt tatttctccc atcagaaatg gtgtagttaa agatttacgt gctgacgtac
     3481 ataaaaaaat gatgcggtta cctttatcat ggtttagcaa tgaacgcaaa ggcgatatta
     3541 ttacaagggt gacttctgat gtgcatgaag tggaatggag tgtgatgagc tctttggaag
     3601 ttgtttttcg cgaccctttc aatatatttt tgtttttagg catgatgatt tttataagtg
     3661 ccaagctcac tgtttttgtt tttattctgc ttcctatagt aggatttctg attggacgta
     3721 ttggcaaaag tcttaaacgt acatcagcca gaagcaaaga aaaacttggg ctgctgattt
     3781 caattataga agaaacacta tcaggcctgc gtataattaa agcatttaat gcagaaaaat
     3841 ttatggcaga aaagtttgca aaagttaatg agcgttataa taccattgcc atacgtgcac
     3901 ttcgcaaaac tgatttatca tcacctctga gtgaattttt aggcactgtc accatgatga
     3961 ttgtaatgta ttttggtggt atgcttgtgt tgggaaaaga tgcatcactt tcggcttcgg
     4021 catttatcac ttatatagca ctattttcgc agattatacc acctgcaaaa tctttcactc
     4081 aggcatttta caatattcaa aaggggcttg cctctgccga cagaatattt gctgttttgg
     4141 aagcagaaga aagtattaag gatcaacagt cggcagaaac aattaaatca ttcaactata
     4201 aaattgaata tcaaaatatt tctttttctt acagaaaagg tgatgaaggt tatgttttaa
     4261 aaaatgtaag tttcactatt gaaaaaggaa aaactgttgc attggtaggc caatcaggat
     4321 caggcaaaac tacattagca gatttattgc ctcgctatta tgatgttgat ggcggtacac
     4381 tgctgattga cggcacagat attaggaaat tacaattgca tagcctcaga gagttgctgg
     4441 gtgttgtctc acaggaacca attcttttta atgacaccat ttacaacaac attgctttcg
     4501 gaatgaacca tgcaacagaa aaaatggttg aagaggctgc aagtgttgcc aacgcacatg
     4561 agtttattac tcaaatgccg gaaggctatc aaaccaacat tggcgacaga ggtagtaaac
     4621 tctccggagg gcagcgtcaa cgtattagca ttgcaagggc agttctaaaa aatccaccga
     4681 tattgattct tgacgaagct acatctgctt tagatactga aagcgaaaaa ttagttcagg
     4741 acgcactgac aagtctgatg aaaaaccgga ctgtattagt cattgcacac agactaagta
     4801 ccattcaaca tgccgatgaa attattgttt tacagaaagg agaaattgtt caacgcggta
     4861 ctcatgttca gcttgcaaat gttgaaggtg tttacaaaaa gctttacgaa ctacaaggtt
     4921 taagttcctg atttgaactt ttacttacct gctaaccact gtttaaaaac gccactacgc
     4981 tctgtactaa ctattgtatc ctgaatggtt gttgggtgaa gtgtaagctt aactcttgat
     5041 ttagaaacaa cattcatttt ttgtattgct ttaaaattaa cgataaactg cctgttgatt
     5101 ctaaaaaaga cagaggggtc aagcattagc tctaattcat ctaagttatg atcaacaata
     5161 aatgattctc caccaaaggt acaaaggtta tttattttat tctcagtaaa aaaataagca
     5221 gcatcttcaa tttctactgt cttaatagtg tctgcaaaac gaattacaat tcgcttctga
     5281 aagccttttt tttcttgcaa cagtgtctgc aacaaagcat cataattaac tccagatgtt
     5341 gagtcagaaa accagttctc atatttttgt attgcagatt ttagttcttc tctttttacc
     5401 ggcttcaata aataatcaat tccgttgtta cgaaaggctt ctattgcata ttcatcataa
     5461 gccgtactga atattaccgg aagctttaca ggatagctct taaaaatttc aaaacttaat
     5521 ccatctgcaa gtcttatgtc tgaaataata agatcgaaat ttttattctg actcataaat
     5581 tcttttccgg aacgtacaga atcaatgact gaaacaatct ccacattagc attaatctcg
     5641 ttcaggagtt taatcagtct gcgtgatgcc ggttcttcgt cttcaaaaat tactatcttc
     5701 attactgatg atttattaat ggcaatgata caacaaaata atcttgtgat gattcaatct
     5761 gtaccgtttt atcagtgagt aaacgatagc gtgcaataat atttcgaagt ccggttcctg
     5821 ttgattcatc tttactcact tttgggtttg tgttattgct aattattatt ctatcgttag
     5881 taaatgcaac atgcacctcc agattttttt ctttagaaat ttcattatgt ttaattgcat
     5941 tctcgagtaa taattgtaat accataggaa caacatattt ttcatcatat aagctatgtt
     6001 cgtcaattaa aagttgcaga tttgaaccaa atcttttttg ttgaataaaa taaaatgcct
     6061 ttgcaatttt aatttcttct gatagtttta ttgtctcttt atccttgtag gtgacaatgt
     6121 ttctgaaaaa tgcagaaaga ttttctacat aatcaac
//
LOCUS       DAOOOL010000135         6051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1370_length_6051_cov_87.421114, whole genome shotgun sequence.
ACCESSION   DAOOOL010000135 DAOOOL010000000
VERSION     DAOOOL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_6051_cov_87.421114"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKU82_09895"
     CDS             complement(<1..185)
                     /locus_tag="PKU82_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83224.1"
                     /translation="MNKIFVIAGIMLLLSVSSYQYKTGWTNEAFADTTDINPNKSSEL
                     SLLMRQMYDHAAEARKAV"
     gene            complement(178..636)
                     /locus_tag="PKU82_09900"
     CDS             complement(178..636)
                     /locus_tag="PKU82_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HOZ83225.1"
                     /translation="MRKLILVAVATVMSLSLMAQDKKVSHFGKKITEENAQPASVVDK
                     MLTSKDTVNVKIQGKIASVCQKKGCWMMMETTDGHTIRVRFKDYEFFVPKDCAGKTAV
                     IEGIAFNDVTSVAELRHYAEDANKSKEEIEKITQPEKKPAFEAEGVIIYE"
     gene            838..3771
                     /locus_tag="PKU82_09905"
     CDS             838..3771
                     /locus_tag="PKU82_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOZ83226.1"
                     /translation="MFLKKWFCCLFTVLLPFVSYSQKKLPGFINPVSYHWADSVLDEM
                     TVDEKIGQLFMVQMQSVWPEKDLDSLESLIRNYHLGGLIVFKGGPYRQALCINRMQQA
                     AKIPLLVSIDGEWGLSMRMDSTIRFPRQMTLSAMHDDSLLNDAFSEIASQCKRMGIQL
                     NFAPDIDINNNPQNPVINTRSFSDDKFEVAEGGILYMQALQNHHVLACGKHFPGHGDT
                     DTDSHYALPVINKSANELDTLEWFPFRQLIRNGVSSIMTGHLNVPLLDDENYPASLSK
                     RIINGYLKDDLDFKGLVITDALNMKGAIVADAKPGDIELQALLAGNDILLMSENVKPA
                     IEKIKTAIQKRKLRKSALDEKVRKILMAKHWAGLYNYTPVDTTNLYYDLNNADASYLN
                     YRLYEKSLTLLRNDAVLPLQNSATERMASLVINDTLENTFQQTLSLFAPVQNFVMSKD
                     ASFATVDSIAAVLSGFKKVIISIHNTTTKPQLNFGITESMISVIQKISMRTKTIVVLF
                     GNSYCLNKLVIPEQTKALIIAYEDTYLPQHITAQALFGAVPFNGRLPVTPGLSYHRGD
                     GLNLQATGVVKYTMPAELNIDLTCLQKVDSIVNDAIKQKAFPGCQVYASVDGKVIYNK
                     SFGYTSYADTQAVENDMIYDIASVTKINATALAAMWLYDHHLLDLKQTIEYYLPDYEK
                     TNKGLLTVEQLMTHTAGLQAWIPFYKEGLTKNGKMMKKYFSSSKHDDYTVQISDSLFM
                     NPKFYSRIDKQIKESPLTEKGKYVYSDLGMLLLQKIIERQAGKKMDVLLNEEFYQPLG
                     LSHLLFNPRSKFPLADIVPTEDDTVFRQTLVHGYVHDPAAALLGGVAGNAGLFANAQS
                     VGVLMQMLLNEGSYAGKQYISKATVDTFTKAPIPGIRRGLVFDKPETNPAKGSPTCKS
                     ASPQTFGHQGFTGTCTWADPSNKLVYVFLSNRVFPSAANQKISKLSVRTEIQQVFYDC
                     LRKVR"
     gene            3836..4975
                     /gene="bshA"
                     /locus_tag="PKU82_09910"
     CDS             3836..4975
                     /gene="bshA"
                     /locus_tag="PKU82_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HOZ83227.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGKALAAKGHRIHFITYSQPVRLD
                     SFSENISYHEVSVSNYPLFEYAPYESALTSMLVDVAMHEGLDVLHVHYAIPHASVAYL
                     AKQILISKGIYIPVITTLHGTDITLVGRDNSYEPVVTFAINQSDGVTAVSESLKRDTY
                     ANFEVTKHIEVIHNFIDLKRFSKKPKDHFKRAIAPGGERLLVHTSNFRKVKRVNDVIQ
                     VFAKIHKRIPSRLLMIGDGDERAGAEKLCRDLQLCDEVRFLGKQDAIEEILSVCDLFI
                     MPSETESFGLAALEAMACQVPVISSNTGGMPELNLDGVTGFTSNVGDIEDMAEKAYHI
                     LKDDQTLNIFKQNALKRASDFDINVILPRYENYYQSVIQNFTVAP"
     gene            5284..5733
                     /gene="rplM"
                     /locus_tag="PKU82_09915"
     CDS             5284..5733
                     /gene="rplM"
                     /locus_tag="PKU82_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOZ83228.1"
                     /translation="MDTLSYKTISLNAKTAKKEWVLIDATDMVLGRLSSEVAKILRGK
                     HRPGFTPHVDTGDNVIIINAEKVKLTGKKMAQKVYVRHTGYPGGQRFTSPEKLMDTYP
                     ERILENAIHKMLPKNRLGNALKKNLRVFAGSEHSHEAQNPRKITLDI"
     gene            5740..>6051
                     /gene="rpsI"
                     /locus_tag="PKU82_09920"
     CDS             5740..>6051
                     /gene="rpsI"
                     /locus_tag="PKU82_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOZ83229.1"
                     /translation="MNNIHKIGRRKTAVARVYLTPGTGTIVINNREFKNYFTTGPLQN
                     KVTQAFAVINANEQFDVKANVMGGGITGQAEAIRLAISRALCEMNPENRPALKKEGLL
                     TR"
BASE COUNT         1893 a         1073 c         1200 g         1885 t
ORIGIN      
        1 acagcctttc gtgcttcagc agcatggtca tacatctgcc gcatcagcag tgaaagttcg
       61 ctgcttttat tgggattaat atcggtagta tctgcaaaag cttcgtttgt ccatcctgtt
      121 ttgtattgat aggatgaaac agaaagcaag agcattatac ctgctatgac aaatatttta
      181 ttcataaata attactcctt ctgcttcaaa tgcaggcttt ttttcaggtt gtgtaatttt
      241 ctctatttct tcttttgatt tgttggcatc ctctgcatag tggcgaagtt ctgctactga
      301 agtaacatcg ttaaatgcta taccttcaat aacagcagtt tttcctgcac aatctttcgg
      361 cacaaaaaat tcataatctt taaaacgaac acgaatggta tgcccatctg ttgtttccat
      421 catcatccag caaccttttt tctgacaaac gctggcaatt tttccctgaa ttttaacatt
      481 cacagtgtct tttgatgtta acatcttgtc aacaacagaa gcaggttgtg cattctcttc
      541 agtgattttt tttccgaaat gagaaacttt tttatcctga gccatcaagg aaagactcat
      601 aactgttgca acggcaacta atatcaattt acgcatgttc ttgaattttg aactgcaaag
      661 gtaaattttt gatttaatta acatacttta tttcactttc tatagtaata aaaccaactt
      721 ttcattaaac aaacatccta aagaaagctt tgtaggcaaa agttttctaa cataaatctg
      781 ttatttactc tgtaatgtaa gtgcaaaaag aatactttat gaaaatacct ttgcctgatg
      841 tttttaaaga aatggttttg ttgcttattc acggtgttgc ttccttttgt ttcgtattca
      901 caaaagaagc tgcccggatt tattaaccct gtatcttatc attgggcaga tagtgtactt
      961 gacgaaatga ctgttgacga aaaaattggt cagcttttta tggtacagat gcaaagtgtt
     1021 tggcctgaaa aagatttaga ttcattagag tcattgatac gtaattatca tctcggagga
     1081 ttaattgttt ttaaaggagg accataccgc caggcacttt gtattaacag aatgcagcag
     1141 gcagctaaaa ttcctttgtt ggtgagtatt gatggcgaat ggggacttag tatgcgcatg
     1201 gacagcacca ttcgctttcc acgtcagatg actttgtccg ccatgcacga tgacagtttg
     1261 ctcaatgatg cattttctga aattgcatct caatgtaaac gtatgggtat tcaactcaac
     1321 tttgctcctg acatagatat caataacaac cctcagaacc ctgtaattaa tacacgctca
     1381 tttagtgatg ataaatttga agtagcagag ggcggaattc tttatatgca agctttgcaa
     1441 aatcatcatg tgcttgcatg tggtaaacat tttcctggcc atggtgatac agatacagat
     1501 tcacattatg ctttgcctgt aattaataaa tcggccaatg aattagacac tttagagtgg
     1561 tttcctttca gacagcttat tagaaatggt gtaagcagta ttatgacagg tcacctgaat
     1621 gttccattac ttgacgatga gaattatcct gcatcattat ctaaaagaat tatcaatggc
     1681 tatcttaagg acgaccttga ctttaaagga ctcgttatta ctgatgcttt gaatatgaaa
     1741 ggtgccattg tagctgatgc caaaccgggt gatattgaat tgcaggcctt gctggcagga
     1801 aatgatattt tattaatgtc ggagaatgtg aaacccgcta ttgaaaaaat taaaacagca
     1861 attcagaaaa gaaaattacg taaaagtgct cttgatgaaa aagtcagaaa aattctgatg
     1921 gccaaacatt gggcaggctt atataattat actcctgttg acactacaaa tctttattat
     1981 gatttaaata atgccgatgc atcttatctc aactacaggt tgtatgaaaa gtcgctgaca
     2041 ttacttcgca atgatgctgt tttaccactt caaaatagtg ctactgaaag gatggcatca
     2101 ttagtaatta acgacactct tgaaaataca tttcagcaaa cattgtcgct ttttgcgcct
     2161 gttcagaatt ttgtaatgtc aaaagatgca tcttttgcca ccgtggacag cattgccgca
     2221 gttctgtctg gatttaaaaa agttatcatt agtatccata acacaacaac caagcctcag
     2281 ttaaattttg gtatcacgga aagtatgatt tcagttatcc agaaaatttc tatgcgcacc
     2341 aaaaccattg ttgtgttgtt tggtaattcc tattgtctga ataaattagt tattccagag
     2401 cagactaaag ctttaataat tgcctatgaa gacacctatc tgccgcaaca cataacagca
     2461 caagcattgt ttggtgctgt tccttttaat ggcaggttgc ctgtaacacc gggcttgtcc
     2521 tatcatcgtg gtgacggtct taatcttcag gcaacaggtg ttgtgaaata tacaatgcca
     2581 gcggagttaa atattgatct tacgtgccta caaaaagttg actctatagt aaatgatgca
     2641 ataaaacaga aagcatttcc tggatgtcag gtatatgcat cggttgatgg taaggttatt
     2701 tataacaaat cgtttggcta tacttcttat gcagatacac aggccgtgga gaatgatatg
     2761 atttatgata ttgcctctgt gacaaaaatt aatgccactg cacttgctgc catgtggctt
     2821 tatgaccatc atcttttaga tttaaaacaa accattgaat attatttgcc ggattacgaa
     2881 aagacaaata aaggattgtt aacagtagag cagcttatga cgcatactgc aggactacaa
     2941 gcatggattc cattttataa agaagggctt accaagaatg gcaagatgat gaaaaaatat
     3001 ttttcttcat caaagcatga tgactatact gttcagatat cggacagtct ttttatgaat
     3061 cctaagtttt attccagaat agataaacag attaaagaat cgcctttaac agaaaagggt
     3121 aaatatgtgt attctgattt aggaatgttg ttattgcaaa agattattga gaggcaggca
     3181 ggaaaaaaaa tggatgtatt gctcaacgaa gagttttatc agccattggg gttaagtcat
     3241 ttattgttta atcctcgcag taaatttcct ttggcagaca ttgtgcctac ggaagacgac
     3301 actgtgttca ggcaaacatt ggtgcatggt tatgtgcacg atccggcagc agccttgtta
     3361 ggaggcgttg ccggcaatgc cggcttgttc gccaatgcgc agagcgttgg tgtgctcatg
     3421 cagatgcttc tcaacgaagg tagctatgcc ggtaagcaat atatcagtaa ggctactgtt
     3481 gatactttta caaaagcacc tattcccgga ataagaagag gtttggtatt tgataaacct
     3541 gaaacaaatc ctgcaaaagg aagccctaca tgcaaaagtg catcaccaca aactttcggt
     3601 catcagggat ttacaggcac ttgcacctgg gccgaccctt ccaataaatt ggtatatgtt
     3661 tttctatcta accgtgtgtt tccttctgca gctaatcaga aaatcagtaa gctaagtgta
     3721 aggacagaaa tacagcaggt gttttacgat tgtttgcgaa aagtacgctg aaagaaacta
     3781 tttttacaac ctgctaaaca aatcaacaat ttgcttgttg attgattctt acagtatgaa
     3841 aataggaata gtttgttatc ccacttttgg aggaagtggc gttgtagcca cagaacttgg
     3901 caaagcactt gctgctaaag gtcatcgcat tcattttatc acctacagtc agcctgtgcg
     3961 gctcgattct ttttctgaaa atatttctta tcacgaagta tcggtaagca attatccgtt
     4021 gtttgaatat gcaccttatg agtcggcatt aacaagtatg ttggttgatg tggctatgca
     4081 cgaagggtta gatgtattgc atgtgcatta tgctattcca catgcatcag tagcatatct
     4141 ggctaagcag attttaattt caaagggaat ttatattccg gttataacga ccttacacgg
     4201 tacagatatt acacttgttg gccgcgataa cagttatgag cctgttgtaa cttttgcaat
     4261 taaccaaagt gatggtgtta cagcagtttc tgaaagttta aagcgtgata cctatgcaaa
     4321 ttttgaagtg accaaacata ttgaagtcat ccataatttt attgacctga aacgtttttc
     4381 aaaaaaacct aaagaccact tcaagcgtgc aattgctccc ggtggtgaaa gacttttggt
     4441 tcatacttct aatttccgga aagttaaaag ggtgaatgat gtaatacagg tttttgcaaa
     4501 aatccataaa agaattcctt cacggttatt gatgattggt gatggcgatg agcgtgcagg
     4561 tgcagaaaaa ctttgtcgcg atttgcaact gtgtgatgaa gtcaggtttt taggtaagca
     4621 ggatgctatt gaagaaatat tatcggtttg cgatttattt atcatgccca gtgaaactga
     4681 aagttttgga cttgctgcat tggaggccat ggcttgtcag gtgccggtta tttcatcaaa
     4741 caccggtggc atgccggaac tgaacttaga tggcgtgaca ggatttacaa gtaatgttgg
     4801 cgatattgaa gatatggcgg agaaagcata tcacatatta aaggacgatc agacacttaa
     4861 tatttttaag caaaatgctt tgaaaagagc ttctgatttt gatattaacg ttatccttcc
     4921 gcggtacgaa aattactatc agtccgtaat tcagaatttt acggttgcgc cataaatacc
     4981 ggcattttta tatgtttttt attgaagaca gttgtcgtaa aacaattaaa attgatggca
     5041 ggaagttgtc cttttgaacc catttcaatg atttgttgtg aataatgtgg caaaaaaatt
     5101 cagcataagt ctgtatttaa atatttgata atcagcaaca tttttaaaac atagtagata
     5161 ggaactgaaa acggttaatt aatattggca aactgttcac actattttgt ttataacgtt
     5221 tgttggtttg aaaaaaagtg ttacttttgc gaccccaatt gtaaaaggtt aatacataat
     5281 actgtggata ctttatcata caaaacaata tcgctgaatg cgaaaactgc caagaaagag
     5341 tgggtgctga ttgatgcaac cgatatggtt ttaggccgtt tatcatccga ggtggctaaa
     5401 attttaagag gcaaacaccg tccaggattt actcctcacg ttgacaccgg agataatgta
     5461 attattatca atgccgagaa ggtaaaactt accggtaaaa aaatggcaca gaaggtatat
     5521 gtgcgccata cgggctatcc cggaggtcag cgtttcacat cacctgaaaa actgatggat
     5581 acctatcctg aaagaattct ggaaaatgcc attcataaaa tgcttcctaa aaacagattg
     5641 ggaaatgctt taaagaaaaa cctaagggta tttgctggca gtgaacatag tcacgaagca
     5701 caaaatcccc gtaaaataac tttagatatt taatcggcaa tgaataatat acataaaata
     5761 ggaagacgca aaacagccgt agcgcgtgtt tatctgactc ccggaacagg aactattgta
     5821 atcaacaatc gtgaattcaa aaactatttc actaccggtc cgcttcagaa taaagtaaca
     5881 caggcatttg cagttatcaa tgcaaacgag cagtttgatg ttaaggccaa tgtaatgggt
     5941 ggaggaatta ccggacaggc agaagctatc cgtttagcta tttcgcgtgc attgtgcgaa
     6001 atgaatcctg aaaaccgtcc ggctttgaaa aaagaaggac tactgacacg t
//
LOCUS       DAOOOL010000136         6000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1384_length_6000_cov_55.364003, whole genome shotgun sequence.
ACCESSION   DAOOOL010000136 DAOOOL010000000
VERSION     DAOOOL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1384_length_6000_cov_55.364003"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PKU82_09925"
     CDS             <1..1171
                     /locus_tag="PKU82_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83230.1"
                     /translation="SITVSTAGNYTVKVTNSNGCSNTSAPMTVTVNSSFQPTITVSGS
                     LSFCSGGNVKLTATKGSSYLWSTGATTRKITVTTAGSYAVTVTQFTTGYVGTSSVVNV
                     NVMPAPSATITASGSTTICQGSSVTLTSSNGANYLWSNGSTSQSISASTAGNYIVTVT
                     GSNGCTKSSSATAVVVNNCGSSCPTPGNLHVTNLRPTSAEINWNLTSANNYYIKVENL
                     NTGYVYYSNALSSGVNSITIGASPSTPYRWSVRGLCGSTYSSWSAYNQFTTPAARMGE
                     GNSNEGLNITPLTTQMPSELEEQPLRIESDDASVTFFPNPATSFVTFNVTASEKNTAT
                     LTLLDLSGRTLNKESYQFNEGLNNGKVDVSNYARGVYMIQLVFDGKTVMKKVILE"
     gene            1286..1825
                     /locus_tag="PKU82_09930"
     CDS             1286..1825
                     /locus_tag="PKU82_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOZ83231.1"
                     /translation="MKFRTIGLMIFTLLYFGQSAKAQQSENIVEDNLRNYYSQILGYQ
                     VKEVFNTKVFDLIDTWLNTPYKYAGKNENGIDCSGFVNVIIKAVYGSSFGCSSVDMYN
                     NLPHVSLKNLTPGDLVFFKTRGRRISHVGMYLGQDKFVHSSTSSGVIVSDLNEPYYKK
                     RFAKGGRISLDESTTSVSK"
     gene            complement(1830..3464)
                     /gene="pruA"
                     /locus_tag="PKU82_09935"
     CDS             complement(1830..3464)
                     /gene="pruA"
                     /locus_tag="PKU82_09935"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOZ83232.1"
                     /translation="MTNAVYNIPPAYNEPVLNYAPGSPEKMALKAALAEARSKQMDIP
                     MYIDGQEVRTGNTSALRPPHDHQHILGMFHKGEKKHVQLAIDAALRAKKQWAEMPWEQ
                     RAAIFLKAADLLAGPYRYKMNAATMLGQSKNAYQAEIDSACEMIDFLRFNVQYMREIY
                     TQQPPVVGKGVWNRTEQRPLEGFVFALTPFNFTAISGNLPTAPAMMGNTVVWKPANTQ
                     IYAANVVMQLLIEAGLPAGVINLVYVSGPDAGDVIFKHADFGGIHFTGSTEVFKGIWK
                     TIGENMPLYKSYPRIVGETGGKDFIIAHKSADIDVLVTALARGAFEYQGQKCSAASRC
                     YIPKSIFEEVKSKLVKEVKSMKLGPVEDFRNFVNAVIDEKSFDKISAYIENAKKSKSA
                     EIIAGGNFDKSKGYFIEPTIILTSDAKYTTMCEEIFGPVLTMYVYEDDKFEETLHLVD
                     NTSEYALTGAIISQDRYAVDVATRALVNSAGNFYINDKCTGAVVGQQPFGGARGSGTN
                     DKAGAMVNLLRWVSPRTIKETFVPAKDYRYPFMEAE"
     gene            complement(3552..>6000)
                     /locus_tag="PKU82_09940"
     CDS             complement(3552..>6000)
                     /locus_tag="PKU82_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83233.1"
                     /translation="ATVRFIHKDGKVHFLKSFFSSNGKDEVRIYAEDITESILLQQEL
                     TNARANVSAVIENTKDLILSTDMNHLITVINHSCQHYLNAVMGKMLNVGDDFRAAMKE
                     EDQMQWSHRHSDVMRGKRVTYNDEISHNGTESYFEFSLQPVVDENGIVTGVSLFGRDV
                     TQRVHFENELVKAKETAETATAAKSQFLATMSHEIRTPLNGIVGMLELLRMTTLNDKQ
                     REYVSTLQLSSENMLSIINDVIDFSKIESDKMELEYEPFELKKVIEETFDLLYYRALG
                     KRLELFYNIENTIPAYVMGDSMRLKQVLVNLVGNAIKFTEKGHILITAQLIPSVSEKL
                     NIKFSVKDTGAGISEEQRQRLFQSFQQADTSTYRKFGGTGLGLTISAKLVALMGGTIE
                     LMSEPGAGSEFYFTVITQPAPIAAARNVRSNLRILRGKKILIFSTNSDFDLHLSDLFN
                     DWHILHQSVTLLEQAKKELSPDANYDALILDAQMQDYLLFAEELKEQIRSSNIPVFAF
                     NARFSGGDIIYGNKLFEAVLPSGVDAIKISSVLTKSFIDSATSFARGDDAVPVFDSQV
                     SLKYPMQILIAEDNPINQTLAVTVLEKLGYKPDVVENGKLVIEKVATKQYDLIFMDVQ
                     MPEMDGMEATTALRKLNNNSIIIAMTAFALEDDKKQCLDAGMNDYTTKPIRIEVVQSL
                     LEKWGDVILKSKAARNDEPLIDKQTIERLKAMAVGDDTSFVKNIFELFLKQMDNLHAD
                     MSKFFNEHDADGMYKASHKLKGSALNIGAKRLSEVCRIIEEKGKLGDLTGMNELIQQL
                     AEVISLTKNEIRSLSLA"
BASE COUNT         1839 a         1235 c         1018 g         1908 t
ORIGIN      
        1 atcaatcact gtttcaacag caggaaatta tacagttaaa gtaacaaaca gcaatggttg
       61 tagtaataca tcagcaccca tgactgtaac agtaaattca tcgtttcaac ctacaataac
      121 tgtcagcggt tcattgagct tttgttccgg tggtaacgta aaattaacag caactaaagg
      181 ttcctcttat ttatggagta caggggcaac aacgcgtaaa attacagtta ccacagccgg
      241 aagttatgct gtaacggtaa cacaatttac aacaggttat gtaggaacat catcagttgt
      301 aaatgtcaat gtaatgcctg caccttcagc aactattacc gcaagtggca gcaccacaat
      361 ctgtcaggga tcaagtgtta cgttaacctc aagcaatggt gctaattacc tttggagtaa
      421 tggctctaca tcacaatcta tttctgcatc tactgccggc aattatattg ttaccgtaac
      481 aggaagtaat ggttgtacta aatcatcatc agctactgct gttgtagtta acaattgtgg
      541 ttcttcttgc ccaacacccg gaaatttaca tgtaacaaac ctcagaccaa cgtctgcaga
      601 gataaactgg aatctgactt ctgcaaataa ctattacatt aaagttgaaa atctgaatac
      661 cggatatgtt tattattcta atgcattatc aagtggtgta aactcaatta ccattggagc
      721 atcaccatca actccttatc gttggtccgt acgtggattg tgtggcagca catactcttc
      781 atggagtgct tacaatcagt ttacaactcc cgcagcaaga atgggcgaag gtaatagtaa
      841 tgaaggttta aatattactc cattaactac acaaatgccg tcagagttag aagaacaacc
      901 acttcgtatt gaatcggatg atgcaagcgt tactttcttc cctaatcctg caacttcatt
      961 tgtaacattt aatgtgactg catctgaaaa gaacacagca acattaacgc tgttagattt
     1021 gtcaggacgc acattaaata aagaaagcta tcaatttaac gaaggactaa acaacggtaa
     1081 agttgatgtg agcaattatg caagaggtgt ttatatgatt caattagttt ttgatggaaa
     1141 aactgtgatg aaaaaagtta ttcttgaata attggttttt attatcataa gtgttaagag
     1201 ccgttatcag attgatgacg gctctttttc atcttagaca atacctgcta aaagaatgcg
     1261 ttcattaaaa tgttatcttt gcccaatgaa gtttcgcaca ataggtttaa tgatttttac
     1321 attgttgtat tttggacaaa gtgccaaagc gcagcaatct gaaaatattg ttgaagacaa
     1381 cctgcgaaat tactacagtc agattttggg ctatcaggtt aaggaagtat ttaacacaaa
     1441 ggtttttgat cttatagaca cttggctgaa tactccatat aaatatgccg gcaaaaacga
     1501 aaatggaatt gactgttccg gatttgttaa cgttatcatc aaagctgttt atggcagttc
     1561 attcggttgc agctctgttg acatgtataa caatctgcca catgtttcgc ttaaaaattt
     1621 aactccgggt gatttggtat tttttaaaac ccgtggacga agaatttcac atgtaggtat
     1681 gtatttgggt caggacaaat ttgtacactc cagcacatca agtggtgtta ttgtaagtga
     1741 ccttaatgaa ccttactata aaaaaagatt cgccaaagga ggaagaattt cccttgacga
     1801 atctactact tccgtgtcta aataaatttt tattccgctt ccataaaagg atatcggtaa
     1861 tcttttgccg gaacaaaagt ttcttttatc gttcgtggtg atacccaccg caacaaattt
     1921 accatagcac ctgccttgtc attagttcct gaacctctgg caccaccaaa tggctgttgg
     1981 ccaacaacag cacctgtaca tttatcatta atataaaagt tacctgcaga atttaccaat
     2041 gctcgtgttg caacatcaac tgcatatcgg tcttgagaaa taattgctcc tgttagagca
     2101 tactcgctgg tgttgtcaac taaatgcaaa gtctcttcaa acttatcatc ctcataaaca
     2161 tacatagtca acacggggcc gaaaatttct tcacacatgg ttgtatattt tgcatcggat
     2221 gtaaggataa tggttggctc gataaaatat cctttgctct tatcaaagtt tccacctgca
     2281 ataatctctg cactcttact cttctttgca ttttcaatgt aagcagaaat tttatcgaat
     2341 gatttttcgt ctataacggc atttacgaag ttgcggaaat cttcaaccgg tccaagtttc
     2401 attgacttta cttctttaac cagcttgctt ttgacctctt caaaaattga cttcggaata
     2461 taacaacgtg atgcagcact gcatttctga ccttgatatt caaaagctcc acgtgccaat
     2521 gcagtcacta atacatcaat atctgcactc ttgtgcgcta taataaaatc tttccctcct
     2581 gtttcgccaa caattcgggg atatgacttg tacaaaggca tattttctcc gatagttttc
     2641 cagattccct taaaaacttc tgttgatcct gtaaaatgaa ttccaccaaa atcagcatgt
     2701 ttaaaaataa catcacctgc atcggggcct gaaacataaa caagattgat tactcctgca
     2761 ggtaaaccgg cttcaatcaa caattgcatt accacattcg ctgcataaat ttgtgtattg
     2821 gcaggtttcc aaacaacagt gttacccatc attgccggtg ctgtaggcaa attacctgaa
     2881 atggctgtga aattgaatgg cgttaaagca aaaacaaatc cttcgagtgg acgctgttca
     2941 gtacggttcc atacaccctt acctactaca ggtggttgtt gtgtataaat ttcacgcata
     3001 tattgtacgt tgaaacgcaa aaagtcaatc atctcacatg ctgaatcaat ttctgcctga
     3061 taagcatttt ttgattgtcc aagcatggta gcagcattca tcttatagcg ataagggcct
     3121 gcaagtaagt cagctgcttt tagaaaaatt gctgcacgtt gttcccaagg catttctgcc
     3181 cattgctttt tagccctaag tgctgcatca atcgctaact gcacatgctt cttttctcct
     3241 ttatgaaaca tgcccagaat atgttgatga tcgtgcggag ggcgaagcgc tgaagtattt
     3301 cctgttcgta cctcttgccc atcaatatac atgggtatat ccatttgttt ggaacgggct
     3361 tctgcaagtg ctgcctttaa tgccattttc tccggacttc cgggtgcata atttaacacc
     3421 ggttcattat aggccggagg aatattataa actgcgtttg tcatacctta attaatttaa
     3481 taatgtgcaa aggtacgaaa atgaaagaag ggcttttgta tccttggtta catcaacaaa
     3541 aagtttaaaa atcaagccaa acttaaactc ctgatttcat tctttgttaa tgaaataacc
     3601 tctgccaact gctgaatcaa ttcgttcatt ccggtaaggt cgccaagttt tcctttttct
     3661 tcaataattc ggcaaacttc agataacctt tttgcaccaa tattgagagc gcttcctttt
     3721 aatttatgcg aagctttata cataccatcg gcatcatgct cattgaaaaa tttactcata
     3781 tcagcatgta agttatccat ctgtttaaga aaaagttcaa atatattttt tacaaaagaa
     3841 gtatcatctc ctacggccat tgccttaagt ctttcaatgg tttgtttgtc aattaaaggc
     3901 tcatcatttc gtgcagcctt actttttaaa atcacgtcac cccacttttc caataatgat
     3961 tgtacaactt caattctgat tggcttagtc gtgtaatcat tcatgcctgc atcaagacat
     4021 tgctttttat catcttccaa tgcaaaagct gtcatggcaa taataatgct gttgttattt
     4081 aacttacgca atgctgtcgt tgcttccata ccatccattt caggcatctg cacatccata
     4141 aaaattaaat catattgctt ggtagcaact ttttcaatca ctaatttacc attctctaca
     4201 acatcaggtt tgtaacccaa tttttccaac actgtaactg caagcgtttg attgattgga
     4261 ttatcttctg ctatcagaat ttgcatcgga tattttaatg acacctgact atcgaaaaca
     4321 ggaacagcat catcacccct ggcaaatgat gttgcactgt caataaacga ctttgttaat
     4381 actgaagaaa tttttattgc atcaactcct gaaggcagta cagcttcaaa aagtttatta
     4441 ccataaataa tatcacctcc tgaaaatctt gcattgaatg caaatacagg aatgtttgaa
     4501 gatcgtattt gttcttttaa ttcttctgca aaaagtaggt aatcctgcat ttgtgcatca
     4561 agaattaagg catcgtagtt tgcatcagga gaaagctctt tctttgcttg ttccagcagg
     4621 gtaacagact gatgtaatat atgccagtca ttaaacaaat cgctcagatg caaatcaaaa
     4681 tcggagttgg tcgaaaaaat caaaatcttt ttacctctta aaatcctgag gttagaacgg
     4741 acattacgtg cagcagctat tggtgcgggc tgcgtaataa ctgtaaaata aaattctgaa
     4801 ccggcaccgg gttcactcat caactcaata gtgccgccca ttaaagcaac taacttggca
     4861 ctgatagtga gaccaagtcc ggtaccacca aactttctgt aagttgaagt atctgcctgt
     4921 tgaaatgact gaaataatct ttgtctttgt tcttcgctaa tgccggcacc ggtgtctttt
     4981 acagaaaact tgatgttaag tttttctgat acagaaggaa taagttgtgc cgtaatgaga
     5041 atatgtcctt tctctgtaaa ctttatggca ttacctacca aattaaccag cacttgtttt
     5101 aaacgcatac tgtcacccat gacataagca ggtatggtgt tttcaatatt atagaataat
     5161 tcaagtctct tacccagtgc gcgataatag agtaaatcaa acgtctcttc aataactttc
     5221 ttcagttcaa atggctcata ttcgagttcc atcttatctg actcaatctt agaaaaatcg
     5281 ataacatcat tgataatact aagcatattc tcactgctca gttgcaaggt actcacatac
     5341 tctctttgtt tatcattaag tgttgtcatg cgcaataact ccagcatccc aacaatacca
     5401 ttaagcggtg ttcggatttc atgactcatt gtggcaagaa actgagattt ggcagcagtg
     5461 gctgtttcag cagtttcttt agctttcacc aattcatttt caaaatgaac tctttgtgta
     5521 acatcacgac caaacaaaga aactcctgta acaatgccat tttcgtcaac aaccggttgt
     5581 aacgaaaatt caaaataact ctcagttccg ttatggctaa tttcgtcatt gtaagttacc
     5641 ctttttcccc tcataacatc actatgacga tgcgaccatt gcatttgatc ttcctctttc
     5701 attgcagcac gaaaatcatc gccaacattc aacatctttc ccattacagc atttaaataa
     5761 tgctgacaag aatgattgat tactgtgatt agatgattca tgtctgttga aagaatcaaa
     5821 tccttggtat tctcaataac agcgctcaca ttggcacgtg catttgttaa ctcttgttgc
     5881 aacaggattg attcagtaat atcttctgca taaattctca cctcatcttt accattacta
     5941 ctgaagaaag atttaagaaa atgaacttta ccatccttat gtataaatct aacagtagca
//
LOCUS       DAOOOL010000137         5973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1389_length_5973_cov_54.743325, whole genome shotgun sequence.
ACCESSION   DAOOOL010000137 DAOOOL010000000
VERSION     DAOOOL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_5973_cov_54.743325"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKU82_09945"
     CDS             <1..1286
                     /locus_tag="PKU82_09945"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83234.1"
                     /translation="PATVSGNKVACATTSDTYTCTAVAGAESYQWSITGDATVSGTTN
                     SVTVNFGPAWTGGTLCVSSQVGCFTSPSKCMTITSSTPAVGAISGSATACPNGVVSFS
                     VAPLAGIQTYNWTTPPNSSVTLGSGTNNVQVTFNGSYNGVGNICVSATSICGVTTAPK
                     CKTVAPGLPSVPASITGGTNGLCNQSGNFSCPLQAGNTYNWTAPGTINNNGSNSVNIQ
                     FSNITTGSVCVSASNSCGSSAYRCVTVKGAPNSPVALTSNPAVICANTTGVEFNANTS
                     NTTGAYTLTWSYTPAAATYNSGGGNTNQLILDWGNTNGVVVVTASNACGSGAKSYTVN
                     LGCRQQGQDDVAALNIYPNPASGTVNINLNAGNQQAVQCIITDLSGKVVMQQTYNVAV
                     GNNTFSADVSHLAKGIYMLQIAGNTMQATQKLIIQ"
     gene            1618..1983
                     /locus_tag="PKU82_09950"
     CDS             1618..1983
                     /locus_tag="PKU82_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83235.1"
                     /translation="MGISQGFITVTLPFMLTQNGFSVALTAGIVAIGISANLWRFVWG
                     PIVDLSLSLHKWYWLSMSASIASLLLICSIPFTIKGETLLSVIVFISQVAATIALLPL
                     NGIMAKQIHENKKGEASGW"
     gene            1964..2767
                     /locus_tag="PKU82_09955"
     CDS             1964..2767
                     /locus_tag="PKU82_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ83236.1"
                     /translation="MKHQGGSLAGSGLGGGAGIWLATHYNLTLAGIVLCMVSVLFALT
                     MFLIKDIPFKKQKSIILEIKGMGINIFSMIKLPVALFVMILILMPIGTGAMSNLWSAV
                     AQDWKADADTVALVTGILSGIVSDVGCIAGGFISDRWGIWNAYLGIGVICALVTVLMA
                     FMPYTPPAYISGVLVYGFCMGLMYAAYTAVLLFVIGKSHSATKFSLMSSLGNMPVVYM
                     TTFNGWAHDKYGSKFMLSAEAFIGIIFIVIFYLILLRMKKKNMIPLIVD"
     assembly_gap    2871..2970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3209..3308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3309..3526
                     /locus_tag="PKU82_09960"
     CDS             <3309..3526
                     /locus_tag="PKU82_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83237.1"
                     /translation="KLPFSNLSAVADYLIFKFSNYQIKPSQLSKLPFSNLSAVADYLI
                     FKLSNYQINTFSNYQIIKSNLPTSMPN"
     gene            complement(3519..4670)
                     /gene="dnaJ"
                     /locus_tag="PKU82_09965"
     CDS             complement(3519..4670)
                     /gene="dnaJ"
                     /locus_tag="PKU82_09965"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOZ83238.1"
                     /translation="MSKRDYYEILGVGRSASEAEIKKAYRQMAIKYHPDKNPGNKEAE
                     EKFKEAAEAYEVLSNAEKKSRYDQFGHQAMGNGGYGGGQHMNMEDIFSQFGDIFGGHN
                     PFESFFGGGGGQRGGRRVNRGSNLRIKVKLTLQEIANGVEKKLKVSKKVACHTCHGSG
                     AQGNNAFTTCSACKGSGQVRRVTNTILGQMQTVTTCNVCGGEGQVIANTCRTCHGTGV
                     EQGEDMITVPIPAGVADGMQLNVSGKGNLGERNGIAGDLLIVIEEIEDPKLKRDGDNL
                     LFDLYITFIDAALGTQVEIPTVDGKAKIKIDAGTQGGKVLRLKGKGLPRVNSYHKGDL
                     LVNINVWTPQQLSAEERKMLEKLRDSENFKPNPGKGDKSFFEKMKEFFN"
     gene            complement(4682..5236)
                     /locus_tag="PKU82_09970"
     CDS             complement(4682..5236)
                     /locus_tag="PKU82_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOZ83239.1"
                     /translation="MKKENEIISNEESSNPEEIMPKQEVIDETSVSEEIQNPLELLKE
                     ELSVANDKYLRLYSDFENYKRRLAKERIELIKTAGADVITSLLPIIDDFERAMKAQEN
                     TSDVTALKEGIQLIFQKLNNTLTQQGLTAIESINKPFDTDLHEAITSIPADDDKKGLV
                     VDEIEKGYMLNDKVVRHAKVVVGS"
     gene            complement(5411..5848)
                     /locus_tag="PKU82_09975"
     CDS             complement(5411..5848)
                     /locus_tag="PKU82_09975"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOZ83240.1"
                     /translation="MGNKAPELAFTSPEGKTIPLSSLKGKIVLIDFWASWCGPCRMEN
                     PNVVAAYNKYKDKKFKGAKGFTVYGVSLDQDKNRWMNAIKQDKLDWPSHVSDLAGWNS
                     KAAQIYRVNSIPANWLIDGNGYIVGSNLRGPALDAAIEKLLAQ"
BASE COUNT         1634 a         1271 c         1019 g         1849 t
ORIGIN      
        1 cacctgctac tgttagtgga aacaaagtag cttgtgccac caccagcgat acttacactt
       61 gtactgccgt tgccggtgca gaatcctatc aatggtctat cactggcgat gccacagttt
      121 caggaactac caacagcgta actgtaaact ttggtccggc atggacaggc ggtacacttt
      181 gtgtttcatc acaagtaggt tgtttcacaa gcccatcaaa atgtatgact attacttctt
      241 ctactcctgc agttggtgcc atcagtggca gcgctactgc atgtcctaac ggtgtagtgt
      301 catttagtgt tgctccgctt gcaggcattc aaacctacaa ctggacaacg cctccaaact
      361 catcagtaac tttaggtagt ggtacaaaca atgtgcaggt aacatttaat ggaagttaca
      421 atggtgtcgg aaatatttgt gtttctgcta ccagcatttg tggtgtaact acagcgccta
      481 aatgtaaaac tgttgctccg ggtcttcctt cagttcctgc ttcaatcaca ggcggaacaa
      541 atggtttgtg taaccaaagt ggaaacttca gttgtccatt gcaagccgga aatacttata
      601 actggactgc accgggcaca attaacaaca atggttcaaa cagtgtaaac attcagttca
      661 gcaatataac tacaggaagt gtttgcgttt ctgcatccaa tagctgcggt agcagtgcct
      721 atcgttgtgt aactgtaaaa ggtgcaccca actctccggt agcacttaca agcaatccag
      781 cggttatctg cgccaacact accggtgttg aatttaatgc caacacttca aacacaaccg
      841 gtgcctatac attaacatgg tcttatactc cggcagcagc aacctacaat agcggtggtg
      901 gaaacacaaa ccaacttatt cttgattggg gaaatacaaa cggtgttgta gtggttacag
      961 cttctaatgc ctgtggcagt ggtgctaaat catacactgt aaacctaggt tgccgtcagc
     1021 aaggtcagga cgatgttgca gcgttaaaca tctatccaaa tcctgcaagc ggaacagtta
     1081 acatcaacct caatgctggc aatcagcaag ctgttcaatg catcatcacc gaccttagtg
     1141 gtaaagttgt gatgcagcaa acatacaacg tagcagttgg caacaacacc ttctctgccg
     1201 atgtttcaca ccttgcaaaa ggaatctaca tgttgcaaat tgcaggcaac acaatgcagg
     1261 caacacaaaa actaattata caataacggt tttgcactaa gcataaatca aatcaaaagg
     1321 ctgtctcaca aaggcagcct tttttttgtg gtagaataaa gtggttgcaa taattattaa
     1381 cttactaaaa tgtaatatca aatagttcaa aaactaattt tagggttgca taaactaatg
     1441 ttgaaattaa tttcattagc acttgatttt taatgttgca cttttattgt tgtcttaata
     1501 gactcacaaa aattaatatc tgaaatcaaa aattaagaga ctgaataaaa tttaaaattc
     1561 aaccacatgc cccaacacta cacaaaacca ttttatatat tttttctaat catgccaatg
     1621 ggtataagcc agggctttat aacggtcaca ttaccattta tgctcacaca gaatggcttt
     1681 tcagttgcac taactgcagg tatcgttgct atcggaattt cagcaaacct atggcgtttt
     1741 gtttgggggc caatagtaga tttatctctt agcttacaca aatggtattg gctaagcatg
     1801 tcagcttcca ttgcttcatt attgcttatt tgttcaattc cgtttacaat aaaaggagaa
     1861 acattgcttt cagtaattgt ctttatatcg caagtagctg ccactatagc tctattacct
     1921 cttaatggga ttatggcaaa acaaattcac gaaaataaaa aaggtgaagc atcagggtgg
     1981 tagccttgcc ggttcaggtt tgggaggtgg agcaggaata tggcttgcca cacactataa
     2041 tcttaccctt gctggtattg ttttatgtat ggtgtctgtt ttgtttgcat taacaatgtt
     2101 tctcatcaaa gatattccct tcaaaaaaca aaaatcaatt attcttgaga ttaaaggaat
     2161 ggggataaac attttttcta tgataaaatt gcccgtggct ttatttgtaa tgattcttat
     2221 actgatgccc attggcaccg gtgccatgtc caatctatgg tcagcagtgg ctcaggactg
     2281 gaaagcagat gcagatacgg ttgctcttgt caccggaatc ttatcgggca ttgtaagtga
     2341 tgtgggttgc atagcaggag gttttatttc agaccgttgg ggaatttgga atgcatatct
     2401 tggcattgga gttatttgtg cattggtaac tgtactcatg gcttttatgc cctacacacc
     2461 accggcatat atttcaggag tgcttgttta tggattttgt atgggattaa tgtatgctgc
     2521 atacactgcc gttcttcttt ttgtaatcgg caaaagccat tcagcaacta aattttcttt
     2581 aatgtcatct ttaggcaata tgcctgtggt gtatatgacc actttcaatg gttgggctca
     2641 tgataaatat ggaagtaaat ttatgctatc tgccgaagca tttataggaa taatatttat
     2701 cgtcattttt taccttatat tacttcgtat gaaaaagaaa aacatgatcc ctcttattgt
     2761 tgattaatcg ggtaaatggc aagttgtgta accttgtgta tatatagctg aaaatcaaac
     2821 taaattaaaa accttcagca ccgcaaactc caacctcagc accgcaaatg nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcagcaccgc aaactccaac ctcagcaccg
     3001 caaatgtcag tgtcttttcc taaataaagt ttacagttta attgcttatc gtgtgtcagc
     3061 gccctttagg cattttgtaa aaagcaaagc ctcttatggc tatgggtgtg cgcaggcttc
     3121 ttgaaaacat taaataacta tgtaaacacc aactttccaa actttccaac tttccaaact
     3181 tcccaacctt ccaaactttc caaactttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnncc aaactcccat tttcaaacct gtcagccgtg gctgattatc tcattttcaa
     3361 attttcaaat tatcaaatca aaccttccca actttccaaa ctcccatttt caaacctgtc
     3421 agccgtggct gattatctca ttttcaaatt atcaaattat caaatcaaca cattttcaaa
     3481 ttatcaaatt atcaaatcca accttccaac ctcaatgcct aattaaaaaa ctctttcatt
     3541 ttttcgaaaa agcttttatc acccttaccc ggattaggtt taaaattttc tgagtcgcgt
     3601 agcttctcaa gcatctttct ttcttctgca gaaagctgct gtggtgtcca cacgttaata
     3661 ttcacaagca ggtcgccttt atgataactg tttactcttg gtagtccttt tcctttcaat
     3721 cgcaacactt tgccgccttg tgtgccggca tcaattttaa ttttcgcttt accgtcaaca
     3781 gttggaattt caacctgagt gcctaatgca gcatcaataa atgtgatgta taaatcgaac
     3841 agcaaattat caccatcacg tttcagcttt ggatcttcaa tctcttctat cactataagc
     3901 aaatcaccgg caatgccatt gcgctcgcca agattacctt ttccgctaac gttaagttgc
     3961 ataccatctg caacacctgc aggaatgggt actgtaatca tatcttctcc ctgctcaaca
     4021 cccgtaccat gacaagtgcg gcaggtattg gcaataacct gtccttcgcc accacaaaca
     4081 ttacaggtag taactgtttg catctgacct aaaatagtat tcgtaacacg tctcacctgt
     4141 cccgaacctt tgcaggcact gcatgttgta aatgcattgt tgccctgtgc accactacca
     4201 tgacaggtat ggcaggcaac tttcttactt acttttaatt tcttctcaac accgttggca
     4261 atttcctgaa gtgtaagttt tactttaata cgcaggtttg agccacggtt aactctgcgg
     4321 cctccacgct gtccgccacc accaccaaag aaactctcaa aagggttgtg tccgccaaaa
     4381 atatctccaa actgactgaa gatatcttcc atgttcatgt gctgtccgcc accataacca
     4441 ccgttgccca tagcctgatg accaaactga tcgtagcggc tttttttctc tgcattactc
     4501 agcacttcat aagcctcagc agcttcttta aatttttctt cggcttcttt attgcccgga
     4561 tttttgtcag gatgatattt aatagccatc tgacggtaag ctttttttat ttcagcttcc
     4621 gaagcactgc gtccaacacc taatatttcg taataatctc tcttcgacat tttgttttct
     4681 attaacttcc caccactacc ttggcatgac gcacaacttt atcattcagc atatagcctt
     4741 tttcaatttc atcaactacc aatccttttt tatcatcatc ggcaggaatg cttgtaatag
     4801 cttcgtgcag gtcggtatca aaaggtttgt ttattgattc aattgctgtc aacccttgtt
     4861 gggttaaggt gttgttaagc ttctgaaaaa ttaattgaat gccttctttt aaagcagtta
     4921 catctgaagt attttcctgt gctttcattg cacgctcaaa atcatctata attggtagca
     4981 gtgatgtaat cacatctgca cctgcagttt ttatcagttc aatacgctct ttcgctaatc
     5041 tgcgtttgta attctcaaag tcgctgtaca atctcagata tttgtcattg gcaacagaca
     5101 actcttcttt caatagttca agaggatttt gtatttcctc acttacagaa gtttcatcta
     5161 taacttcttg tttcggcatt atttcctcag ggtttgagga ttcttcatta gatataattt
     5221 cgttttcctt tttcattttt ctacgcttaa acaatttcat cgctgatttt caaaaacatt
     5281 gccatttaac aattggggtc aaaatggcag gcaaagatag cttttacccg gctgcaactg
     5341 tcaaacagga ggtaaaatga caacagccgg aatttccggc tgttgtcatt cttcgataat
     5401 aaaatattat ttattgtgcc aataattttt cgattgctgc atctaaagca ggccctctta
     5461 aattactacc cacaatatat ccgtttccat caataagcca gttggcagga attgagttaa
     5521 cacgataaat ttgcgctgct ttactattcc atcctgcaag atcactcaca tggctaggcc
     5581 agtctaattt gtcttgctta atagcattca tccaacgatt tttatcctga tcgagcgata
     5641 cgccataaac agtaaaacct ttggcacctt taaatttttt gtctttatat ttattatagg
     5701 cagctaccac attaggattt tccatacggc atgggccgca ccatgatgcc caaaaatcta
     5761 ttagcacaat ttttcctttc agtgatgaaa ggggaatagt ttttccttct ggtgatgtaa
     5821 atgccagctc cggtgctttg ttgccaatgg caatgccttc aaccggcttt tgtgcctgtg
     5881 caaaaaaatg cagtgccgat gattgccagc gataagaatg ttgctttaag tgtagttgtc
     5941 atatttttaa ttttgtttat ggctgccaac tat
//
LOCUS       DAOOOL010000138         5939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1404_length_5939_cov_66.808464, whole genome shotgun sequence.
ACCESSION   DAOOOL010000138 DAOOOL010000000
VERSION     DAOOOL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_5939_cov_66.808464"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2650
                     /locus_tag="PKU82_09980"
     CDS             <1..2650
                     /locus_tag="PKU82_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOZ83241.1"
                     /translation="NKGVSVKKVTAILEDYKKNKWFSTYGNGMCLIDHGHFFTLTKDE
                     GLTDNNILCMKEDNEGNIWIGTPRGLCRYSGGRFITYTTENGFNDNRLLTAYTDHLNR
                     IWFGIVNGGLNYIHGNTVYTPPFENILARATIWSIAQDAAQNFWFGTSTGPALVSTDL
                     SKISFPFDLLNNKIIYAVMAHRNGSLWFGTDRGIYIYENNNLRVLNKLNGLQNDNIRY
                     LFQDRQGLVWVGTLQGMYFMHNETAVSLNDKLNLNKTPITSITQDIEGNIIFSTFDFG
                     VYIFSKKKGTTAIRHLEVGNGLFNNRILTVASDNNYLWMGTPLGIDRIDWNTYLKRDF
                     TNIIHFDKSNGFYGVETNAATYDKDGFLWFATVNGAVRYNPNSGYSKSTTPVVLLQSI
                     KAYMREVNWSEQGFSIDSITGLPVNPVFPYGYNNLNFHFTGIYFASPDALQYRWKLDG
                     FDDYWVPATNLNIANYSNLPPGNYTFQVQATANGHDWSPSLTYDFKVKSPVWRTNFFY
                     FIYFVALTLLILVILKLRTRSLRNAQLLLRQKINERTHQLRENNMQLAKLSIVASETD
                     NAVMIFDHNLNLEFVNEGYTRMHGYTMDEIIRKQGSKLTQLSYNENIKTIIKDCLEEH
                     KSVIYETKITHRNGQQLWTSSTLTPVFNDKDELSNIVVIDTDITLHKKMEEQIRESLD
                     EKNILLKEIHHRVKNNLQIIVSLFNLQSAYLQDKASHKILKEGQDRIRSMALIHDRFY
                     QSDGTSRIDFDDYIKRLCETIMQSQNANPQKIRLQIEAEKISLDIDTAVPCGLIINEL
                     VSNAIKHAFADDGGTIWVTFKKVDDHYLLSIADNGKGLPDGKDFETADTLGIQLVNVL
                     SDQIDGKVTVENSNGLKVIIKFKPSY"
     gene            2662..3816
                     /locus_tag="PKU82_09985"
     CDS             2662..3816
                     /locus_tag="PKU82_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ83242.1"
                     /translation="MDKIRILVVEDESIVAKDIQQTLIRLGYDVPATASSAQNAYARL
                     EEHEPDLVFLDIKLKGDLDGIHIAEHIKQKYDIPVIFLTSFVDKNTLDRAKITEPYGY
                     LVKPFNESDLQTTVEMALYKFQKDLQVRENERRFANALANVEEAILVADTEFRITFLN
                     PKAESMTGFGNESAQGMSLFKLIKIEGDEYEIVNADNLGKLMKQDEVLSIKQSNITIV
                     RDYSNIKADFTCSPIRDEKNQLLGYAVVIGSSKTEKSVAQPVIKPTEEKLVEAPSNPL
                     ENQVIQNSFFVKRGAMLVKVFLENIYWLQAMDNYVIIQTKEDQFVIHATMKDIETKLP
                     PEKFMRVHRSYIIALDKISHMDEGAVVINDKTIPIGKSYKEVFMNRINFL"
     gene            complement(3842..4585)
                     /locus_tag="PKU82_09990"
     CDS             complement(3842..4585)
                     /locus_tag="PKU82_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOZ83243.1"
                     /translation="MIKAVIVDDEQHCINRITTLLKDYENEVQLVATASTVKEGLNII
                     KKVQPDLLFLDVEIADKTGFDLLREIESPSFDVIFTTAHDKYAVMAFKFCAIDYLLKP
                     VDESDFAIAMKKILDSDDRQDMTRQLSELIKGAGLNKPRKIGVPTLNGIVFVLISDIR
                     LCRADGNYTEIFLNNNHKIVVSKTLKEFDDLLSDYQFFRVHNSNLVNLDYVKSYNKGK
                     GGFLILDDDTNVEVSSRRKDELLKKLSLI"
     gene            complement(4597..>5939)
                     /locus_tag="PKU82_09995"
     CDS             complement(4597..>5939)
                     /locus_tag="PKU82_09995"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ83244.1"
                     /translation="AIVYSEMNQHNKAIEFYQKALEINREIKNNFGIASNYINIGTDL
                     NHLNRFSEANHYMLLAEELFNKIGDQSSEAMAIKARGELILNAPDSVLKSLNIPKTQK
                     YTVSLELLKRASAMFAEIGDISNRSDICQDLSMIYEYQGDAKNSLAYYKQHISLRDSV
                     MLDNKKAEIERTELKFNFELKEKELNLINSKIASDLQLAEQKRKYLMMGSVLILLITG
                     GGFVSYKLRKEAQQKRKDAEFKLANLNAELKSLRLQMNPHFMFNSLNSIADFIDKNES
                     RIASNYTVKFAKLMRMILEYSEQQEITLEEELKLLELYLSLEALRINPNLSYVISVSP
                     EIDISNILIPPMMVQPFVENSILHGIIPLAKPGVIKIDVSVSIDELIVIVEDNGVGIN
                     SSMKSDNKKSMAMKITKDRIALFNEQHSAAASVNLINLNQGTRIELRLPVIHRF"
BASE COUNT         1992 a         1073 c          997 g         1877 t
ORIGIN      
        1 caacaaaggt gtttcagtta aaaaagttac tgccatcctt gaagattata agaagaataa
       61 atggttcagt acttatggta atggcatgtg cctgatagat catggtcatt ttttcacgtt
      121 gacaaaagat gagggtctca cagataacaa tattttgtgc atgaaagaag ataatgaagg
      181 aaacatttgg attggaacac ctcgtggttt gtgtcgttat tcaggtggac gctttatcac
      241 ctacacaaca gaaaacggat ttaatgacaa cagactactt actgcataca ccgatcactt
      301 aaacagaatt tggtttggca tcgtaaatgg cggattgaat tacattcatg gaaatacagt
      361 ctatacacct ccttttgaaa atattcttgc acgtgccaca atttggagta ttgcacagga
      421 tgcagcacaa aatttctggt ttggaacatc aaccggtccg gctttagttt caactgactt
      481 atctaaaatt tcttttccat ttgatttact taacaataaa attatttatg ctgttatggc
      541 acaccgtaac ggtagtttat ggtttggcac cgaccgtggt atttatattt acgaaaacaa
      601 caatctgcgc gttttaaata agctcaatgg cttacagaat gacaacattc gctatttatt
      661 tcaggacaga caaggcttgg tttgggttgg cactttgcaa ggcatgtact ttatgcataa
      721 tgaaacagca gtttcactta acgacaagtt gaatcttaat aaaacaccta tcacttcaat
      781 cacacaagat atagaaggaa atattatctt ttcaactttt gattttggtg tctatatttt
      841 ttcaaaaaag aaaggaacaa ctgccatcag acatcttgaa gttggaaatg gtttgttcaa
      901 caatagaatt ttaactgttg caagcgacaa caattatttg tggatgggta ctccacttgg
      961 cattgacaga attgactgga acacttatct gaaaagagat ttcacaaaca tcattcactt
     1021 tgataaaagt aatggatttt atggtgttga aaccaatgca gctacttacg ataaagatgg
     1081 ctttctttgg tttgctactg tcaacggtgc tgttcgatac aatccaaatt ccggttacag
     1141 taaaagtaca actccagtag ttctgctgca atccatcaaa gcctacatgc gcgaggtaaa
     1201 ttggtcagaa caaggttttt ctattgacag cattactggc ttacctgtga atccggtttt
     1261 tccgtatggc tacaacaatc ttaattttca ttttacagga atttattttg catcaccgga
     1321 tgctttacaa tacagatgga aacttgacgg ctttgatgac tactgggtgc ctgctaccaa
     1381 cttaaacatt gcaaattact ctaacctgcc acccggaaac tatacatttc aggtgcaggc
     1441 aacagccaac ggtcatgact ggagtccatc acttacctac gactttaaag taaaatcacc
     1501 tgtttggcgg acaaactttt tttattttat ctacttcgtt gcattaacat tactcatttt
     1561 agttattctg aaattaagaa caagaagttt acggaatgca caactactat tgagacagaa
     1621 aatcaatgag cgtacccatc agcttaggga aaacaatatg caacttgcga aactttcaat
     1681 agttgccagc gaaacagaca atgctgttat gatttttgac cataacctga atcttgaatt
     1741 tgtgaatgaa ggttatacac gcatgcatgg ctacactatg gatgaaatca tacgtaagca
     1801 aggcagtaag ctaacacagc tttcgtataa tgaaaatata aaaacaatca ttaaagactg
     1861 ccttgaggag cataagtcag ttatttatga aactaaaatt acacatcgca acggacaaca
     1921 actttggact tccagtacgc tgacaccggt atttaacgac aaagatgaat tgagcaatat
     1981 tgtggtaata gacacagaca tcaccctgca taaaaagatg gaggagcaaa ttcgtgaatc
     2041 gttagacgaa aagaatattc tgttaaaaga aatacatcac agggtaaaaa acaatttgca
     2101 gattattgta agcctgttca acttacaaag tgcttatttg caggataagg catcgcataa
     2161 aatcctcaaa gaaggacaag acagaatacg ttctatggct ttgatacatg atcgctttta
     2221 tcagagtgac ggaacatcac gaattgattt tgatgactac atcaaacgcc tttgcgaaac
     2281 cattatgcaa tctcaaaatg ccaatcctca aaaaatacgt ttgcagattg aggcagaaaa
     2341 aatatctttg gacattgata cagcagtacc atgtggattg ataattaatg aacttgtaag
     2401 caatgctatt aaacatgctt ttgcagatga tgggggaaca atttgggtga catttaaaaa
     2461 ggtagatgac cattatctgc taagtattgc tgacaatgga aaaggattac ctgatggaaa
     2521 agattttgaa actgcagaca cattaggtat tcaattagtg aatgtgttga gcgatcagat
     2581 tgacggaaaa gtaacagtag aaaacagtaa cggattaaaa gtgataatta aatttaaacc
     2641 atcgtattaa atttttcaat tatggacaaa ataagaattt tagttgttga agacgagagt
     2701 atcgttgcaa aagacataca gcaaacatta attcgtcttg gatatgatgt tccggccacg
     2761 gcatcatcgg cacaaaatgc ctatgcaaga cttgaagaac acgaacccga tttggttttt
     2821 cttgacataa agttaaaagg cgatttggat ggaattcaca ttgcagaaca cataaaacag
     2881 aagtatgata ttcctgtaat atttctcact tcttttgttg acaaaaacac gctggacaga
     2941 gctaaaatca ctgagcctta tggctatttg gtaaaaccat tcaacgaatc tgacttgcaa
     3001 acaactgttg aaatggcatt gtataaattt cagaaagatt tgcaggtgcg tgaaaatgaa
     3061 agacgctttg ccaatgctct tgcaaatgta gaagaagcca ttttggttgc cgatacagag
     3121 ttccgtatta catttttaaa tccaaaagca gaaagcatga caggcttcgg caatgaaagt
     3181 gcgcaaggca tgagtttgtt taaacttata aaaattgaag gtgatgaata cgaaattgtc
     3241 aatgccgaca acttaggtaa actcatgaaa caggatgagg ttttaagtat taagcagagt
     3301 aatataacta ttgttagaga ttacagtaat ataaaagctg actttacatg ctcccctatt
     3361 cgtgatgaaa aaaaccaatt actaggctat gctgttgtca ttggttcatc taagacagag
     3421 aaatctgttg cacagcctgt aataaaacct acagaagaaa aacttgttga agcaccttca
     3481 aaccctctag agaatcaggt tattcaaaac tcattctttg taaaaagagg tgcaatgctt
     3541 gtaaaagtat ttcttgaaaa catttactgg ctgcaggcaa tggacaatta tgtaattatt
     3601 cagactaaag aagatcagtt tgtgattcat gccacaatga aagatattga aaccaaactt
     3661 ccacccgaaa aattcatgcg cgtacaccgc tcctacatta ttgccttaga taaaataagt
     3721 cacatggatg aaggtgcagt agttatcaat gacaaaacca ttcctattgg caagagttac
     3781 aaagaagtgt ttatgaacag aattaatttt ctgtaaacaa aacttttcag atgtaattga
     3841 tttatatcag gcttaacttt tttagcagtt cgtcctttct gcgtgatgac acctcaacat
     3901 tagtgtcatc atcaagaatc agaaaaccgc ctttaccttt attataactt ttaacatagt
     3961 ctaagtttac tagatttgag ttatgtactc tgaaaaactg gtaatctgaa agtaaatcat
     4021 caaactcttt taatgttttt gaaacaacaa ttttgtgatt gttatttaaa aaaatctcag
     4081 tataatttcc atctgcacga cataatctta tatcagaaat caaaacaaac acaattccat
     4141 tcaatgtggg tacaccaatt tttctcggtt tatttagtcc tgctccttta atcaactctg
     4201 acagttgcct tgtcatatcc tgccggtcat cgctatcaag aatcttcttc atggcgattg
     4261 caaaatcgct ctcatcaaca ggtttcagca aataatcaat tgcacaaaat ttaaatgcca
     4321 taacagcata tttatcatgt gctgttgtaa aaattacatc aaatgatgga gactctattt
     4381 ctcttaataa atcgaagccg gttttatctg caatttcaac atctaaaaaa agcaaatcag
     4441 gctgaacctt tttaataata ttcaaacctt ccttaacagt actggctgtt gcaactaact
     4501 gcacctcatt ttcataatct ttaagcagtg tagtaattct gttaatacaa tgctgttcat
     4561 catctacaat tactgccttt atcatttacg attctattaa aatctatgaa ttacaggtaa
     4621 acgcaattct attcttgtac cttgatttaa attaattaaa ttaactgatg cagcagcact
     4681 atgctgttca ttaaaaagtg caattctgtc tttagtaatt ttcattgcca ttgatttttt
     4741 attgtcagat ttcattgagc tatttattcc aactccatta tcctcaacaa taacaatcaa
     4801 ttcatcaata gaaacagaaa catcaatttt gataacacct ggttttgcca atggaataat
     4861 accatgtaaa atactatttt caacaaaagg ctgaaccatc attggaggga tgagaatatt
     4921 acttatatca atttccggtg atacagaaat cacatagcta agattggggt tgattcgtaa
     4981 tgcctctaac gaaagatata actccaacaa tttcaattct tcttctaaag ttatctcctg
     5041 ctgttctgaa tattctaaaa tcatccgcat gagttttgca aatttaacag tataattaga
     5101 ggctattcgc gactcattct tatcaataaa atcagcaatt gaattcaatg aattaaacat
     5161 aaaatgtgga ttcatttgca gccttagcga tttcaactct gcattaaggt ttgccaattt
     5221 aaactctgcg tccttccttt tttgctgtgc ttcctttctt aatttatagg aaacaaatcc
     5281 ccctcctgta atcaacaaaa tcaagacact gcccatcatc agatattttc tcttttgctc
     5341 tgctaactga aggtcagatg ctatttttga attaatcaaa tttagctcct tctcttttaa
     5401 ttcgaagttg aattttagct ctgtcctctc tatttctgct ttcttattat caagcataac
     5461 actgtcgcgt aatgatatat gttgcttata ataggctaaa gaatttttag catcaccttg
     5521 atactcgtaa atcatgctta aatcctgaca tatatctgac cgattagata tatctcctat
     5581 ttctgcaaac atagccgatg cccttttcag aagttccagg ctaacggtat atttctgtgt
     5641 ctttggtata tttaaagact ttagtacaga atcaggtgca ttgagaatta attcacctct
     5701 tgcttttatg gccatagctt cagagctctg atcaccaatt ttattgaaca actcttcagc
     5761 cagcagcata taatggtttg cttcagaaaa ccgattgaga tgattaaggt cggttccaat
     5821 atttatataa ttactggcaa taccaaaatt attctttatt tcacgattaa tttctaatgc
     5881 tttctgataa aattcaattg ctttattatg ctgattcatt tcactgtaaa caattgcaa
//
LOCUS       DAOOOL010000139         5932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1406_length_5932_cov_42.376893, whole genome shotgun sequence.
ACCESSION   DAOOOL010000139 DAOOOL010000000
VERSION     DAOOOL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1406_length_5932_cov_42.376893"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PKU82_10000"
     CDS             <1..701
                     /locus_tag="PKU82_10000"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ83245.1"
                     /translation="TNAIIKIIPDMYDILSGSVKMNSIFGAPLIEIYPQLMPAWQQYA
                     KRLMDIIVSFVFLILLTPTFIITALIVILTSKGPVIFKQERIGLHGNPFNIYKFRSMY
                     LDAESGGPKLSSKDDSRITPWGKIMRKFRIDETPQFFNVLIGEMSLVGPRPERQFFID
                     QIMLHSPHYRHLHKVRPGITSWGQVKFGYAENVGQMVERLKYDIIYIENMSLALDFKI
                     LFYTVKIIFQGRGK"
     gene            767..1657
                     /locus_tag="PKU82_10005"
     CDS             767..1657
                     /locus_tag="PKU82_10005"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOZ83246.1"
                     /translation="MKHIAVIGSGTMGNCIAHVCAQYGNTVSLIDISEASLEKAIKTI
                     TGNFDRMVTKGIVTTADKDAAIQRITTYTKLSDGVKNADLIIEAASENESIKLGIFKE
                     LDSVCKADAILATNTSSISITKIASVTKRPDKVIGMHFMNPVPVMKLIEVIRGYKTSD
                     EVTKIIFDLSGKLNKIPVEVNDYPGFIANRILMPMINEAIYSLYEGVAGVHEIDEVMK
                     LGMAHPMGPLQLADFIGLDVCLAILRVLHDGFGNPKYAPCPLLVNMVTAGNLGKKSGV
                     GFYDYSSGGKDLVVAATFQK"
     gene            1658..2830
                     /locus_tag="PKU82_10010"
     CDS             1658..2830
                     /locus_tag="PKU82_10010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOZ83247.1"
                     /translation="MALPYPEVRLKKGKEKSVLNFHPWIFSGALEKMPAQLNNGDAVT
                     LLSHQGEILGTGLFHHSSIAVRLLAFSKVELNAAFWFQKLSNALQLRKNIHLFNNKQT
                     TAYRLVHGEGDGQSGLIIDIYGDCAVIQCHIKGMFRHRVMIAEALDKIFNQSLNTIYD
                     KSEDSFFENNEDRFLKGEKQSEVIRENGHQFYVNWFEGQKTGFFIDQRENRKLLGNYC
                     DGKNVINLFAYSGGFSIYALKSGAAMVHSVDSSIRAENWANQNVQLNDAINHQFFCND
                     VFTFLKETKSTYQVWVVDPPAFAKRLDAVRNAMIGYRNLNTAVFRKAPAGSIIFTFSC
                     SQAVDALTFRKVIFQSAKEAQRQIKILHILSQPADHPVSVFHPEGEYLKGLVLYVE"
     gene            2874..3674
                     /gene="kdsA"
                     /locus_tag="PKU82_10015"
     CDS             2874..3674
                     /gene="kdsA"
                     /locus_tag="PKU82_10015"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOZ83248.1"
                     /translation="MPQLKHTASENFFLMAGPCIVESEQMCMDIAGTVKEITDRLQIP
                     YIFKASFRKANRSRLDSFTGIGDVPSLEILRKVRERYQLPVVTDIHESHEAALAADYV
                     DVLQIPAFLCRQTELLVAAARTGKTVNVKKGQFLSPESMKFAIDKIRQSGNNQVMVTE
                     RGTMLGYGDLVVDMRGIPEMKKFNVPVVMDVTHSLQQPNQSSGVTGGKPELISTIARA
                     AMAAGADGIFIETHPNPAKALSDGANMLRLDLLEKLLVSLLKVQQAVR"
     gene            3834..4505
                     /locus_tag="PKU82_10020"
     CDS             3834..4505
                     /locus_tag="PKU82_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOZ83249.1"
                     /translation="MNSKLIIFIVISAVHLVAHLMAFQWLVFITKPLLMPILLWYFTE
                     GLSFGKLTLIQKRMITALTLAWIGDIVNLYSQINHWIFMAGVIIFLAMQIMYIIICFY
                     LIRFKQINQLYFIFSTLLFIAYGSALLWILLPHLNELTIPVLIYASALTLTGIASALT
                     KDQLPRNVFYCLLTGTLLYMTTDSLDAINNFKASFPYAAFWIALTYLSAQMLITIAVK
                     KIKTI"
     gene            complement(4727..5626)
                     /gene="nusB"
                     /locus_tag="PKU82_10025"
     CDS             complement(4727..5626)
                     /gene="nusB"
                     /locus_tag="PKU82_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOZ83250.1"
                     /translation="MQVLYTYFQSEGYNVTKAEKELVEGTSRLYELYLALLQLLIELA
                     EQEQLYRIDVASKFIVNKREFVRSFEKTEFISWLKNDEQFLDNCKKYKISWQADMEAV
                     GKCFYKLRQHPAYREFTMTNTEVVEMDWLLKLYKEEVQQSESINGVIEEKNIWWAESL
                     ELAHVMVVKTIRQFYTTDQQAILPLFKDEADDRDFMIRLLKETIKNDKEWTSIIAERT
                     KNWEVDRIALSDMIMLKMAVTEVLKFDQIPVKVTLNEYIDISKDYSTPQSKVFINGVL
                     DKIVEELRKQDKVKKTGRGLVEN"
     gene            complement(5707..>5932)
                     /locus_tag="PKU82_10030"
     CDS             complement(5707..>5932)
                     /locus_tag="PKU82_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HOZ83251.1"
                     /translation="MDKGILWAPDFLINAGGLINVYIELKKFSRAEALKQTEHIYNVT
                     LEIFKKSKTEKIPTLVAAMQMAEKRIYGGK"
BASE COUNT         1886 a         1094 c         1053 g         1899 t
ORIGIN      
        1 acactaatgc cattattaaa attatacctg atatgtatga tattctttca ggttcagtaa
       61 aaatgaattc tatttttggt gcaccactca tagaaattta tccgcaactg atgcctgcat
      121 ggcaacaata tgccaagcga ttgatggata ttatagtatc ttttgtattc ttaatcctac
      181 tcaccccaac atttatcatc actgcactca tagtaatact gacaagcaaa gggccagtaa
      241 ttttcaagca agaacggatt ggactgcacg gtaatccttt caacatttat aaatttcgca
      301 gcatgtatct tgacgctgaa tccggtgggc ctaaactttc aagtaaggac gacagcagaa
      361 ttacaccatg gggaaaaatt atgcgcaagt tcaggattga tgaaacacca cagtttttca
      421 atgttctcat tggagaaatg tcgcttgtgg gtccacgtcc tgaaagacaa ttttttattg
      481 atcagataat gcttcactct ccgcattaca gacacttgca taaagttcgt cccggaatta
      541 catcatgggg tcaggtaaaa tttggctatg cagaaaatgt tggtcagatg gttgaacgat
      601 taaagtatga tataatttat attgaaaaca tgtcgctggc acttgacttt aaaatacttt
      661 tttacactgt aaaaattatt tttcaggggc gcggtaaata atgctaataa caatttaaca
      721 tcaaaaaata ttttcaaact acaaacctta aataaaaaat ttcatgataa agcatattgc
      781 agtaatcgga tcgggaacaa tgggaaattg tatagcgcat gtatgtgcac aatatggaaa
      841 caccgtttcg ctgatagaca tttctgaagc gtcattagaa aaagctatca aaaccattac
      901 aggaaacttc gaccgtatgg taacaaaagg aattgtgact acagcagata aagatgcagc
      961 aatacaacgc ataactactt acaccaaatt gtctgatgga gttaaaaatg cagaccttat
     1021 tattgaagca gcttcagaaa acgaaagcat aaaacttggc atatttaaag agttagacag
     1081 cgtttgcaaa gccgatgcta ttttagcaac aaacacttct tcaatttcaa taacaaaaat
     1141 tgcatctgta accaaaagac ctgataaagt tattggaatg cattttatga atcccgtacc
     1201 tgtgatgaaa cttattgaag ttattcgtgg gtataaaaca agtgatgaag taacaaagat
     1261 aatttttgat ctgtcaggta aactcaataa aattcctgtt gaagtaaatg actatcccgg
     1321 atttattgcc aacagaattt taatgccaat gattaacgaa gctatctatt cactttacga
     1381 aggtgttgcc ggagtgcatg aaattgatga ggtaatgaaa ttaggtatgg cacatcctat
     1441 gggcccgttg caattggccg attttatagg ccttgatgtt tgccttgcta ttttacgtgt
     1501 attgcatgat ggttttggaa atcctaaata tgcaccatgc ccattattag taaatatggt
     1561 tacagcaggt aacttaggta aaaaatcagg tgtaggtttt tatgattact catcaggtgg
     1621 aaaagacctt gttgtagctg caacatttca gaaataaatg gcactaccct atcctgaagt
     1681 ccgtttaaaa aaaggaaaag aaaaaagtgt attaaatttc catccctgga ttttttctgg
     1741 tgctttggaa aaaatgcctg ctcaacttaa taatggtgat gccgtgacgc tactttcaca
     1801 tcagggtgaa atacttggca caggactgtt tcatcattca agtattgctg tcagacttct
     1861 tgctttttct aaagttgaat tgaatgctgc attttggttt caaaaattat ccaatgcctt
     1921 gcaactaagg aaaaacattc atttgtttaa caataaacaa accacagcat atcgtttggt
     1981 acatggcgaa ggcgatggac aatcaggatt aattattgac atttatggcg attgtgcagt
     2041 gatacaatgc cacataaaag gaatgtttag gcatcgtgtt atgatagctg aagcgttaga
     2101 taaaattttc aatcaatcac taaacaccat ttatgataag agcgaagatt cattttttga
     2161 aaacaatgag gacagattcc ttaaaggcga aaagcaatcg gaagtaataa gagaaaacgg
     2221 acatcagttt tatgtaaact ggtttgaagg tcagaaaaca ggatttttta ttgatcagcg
     2281 tgagaacaga aaactgttag gtaattattg tgacggaaaa aatgtaatca acctttttgc
     2341 ttattcagga ggtttctcca tttatgcatt aaagtcaggt gctgcaatgg ttcattctgt
     2401 tgacagttct attcgtgcag aaaattgggc aaatcaaaat gttcagttaa acgatgctat
     2461 taatcatcaa tttttttgta acgatgtatt tacatttctg aaagaaacaa aaagcactta
     2521 tcaggtttgg gtagttgacc cacctgcatt tgccaagcgg cttgatgctg ttagaaatgc
     2581 tatgataggc tatcggaatc tgaacactgc tgtttttcgc aaagcacctg caggaagtat
     2641 cattttcacc ttctcttgct cgcaggctgt tgatgcatta accttcagga aagtaatttt
     2701 tcagagtgca aaagaagcac aaaggcagat aaaaatcctg cacatacttt ctcaacctgc
     2761 cgaccatcct gtttcagttt ttcatcccga aggcgaatac ctaaaaggtt tagttttgta
     2821 tgtagaatag caccatttta atttaatcac attaagtcaa tatgcttgag aatataccac
     2881 agttaaaaca tacagcttca gaaaattttt ttcttatggc cggaccatgt attgttgaat
     2941 cggaacagat gtgtatggac attgcaggaa cagtgaaaga aattactgac agacttcaga
     3001 ttccatatat ctttaaagca agtttcagaa aagccaaccg ctcaagactt gattcattta
     3061 ccggcattgg cgatgttcct tctcttgaaa tactgcgtaa agtccgggag cgttatcagc
     3121 tacctgttgt taccgatatt cacgaaagcc acgaggctgc acttgcagca gattatgttg
     3181 atgtactaca gattccagca tttctttgtc gccagacaga attgttggtg gcagcagcac
     3241 gaactggaaa aactgttaac gtaaaaaaag gacaatttct ttctcccgaa tctatgaagt
     3301 ttgccataga taaaattcga caatcaggca acaatcaggt aatggttacc gagcgcggca
     3361 ccatgcttgg ctatggagat ttagtggttg acatgcgtgg tattcctgaa atgaaaaaat
     3421 ttaatgtacc ggtcgtaatg gacgttactc attcattgca acagcccaat cagtcatccg
     3481 gtgtaacagg aggtaaacct gaacttatca gcactattgc ccgtgctgca atggctgccg
     3541 gtgccgatgg aatttttatt gaaacgcatc caaacccggc caaagcattg agtgatggtg
     3601 ccaatatgct acgtctcgac ctgcttgaaa aacttttagt ttcattacta aaagtgcagc
     3661 aggctgttcg ctgatgtgat atgactgcat aacacattat ctttttgtct tttttccaat
     3721 cttagatttg gacaattgta tttttatttc atactttaca tcgccattta tacaatagat
     3781 taagaaaaag tggcaagtga aagtaaaaca atgatcatat caaaacagat acaatgaaca
     3841 gcaagctgat tatatttatt gttatatcag cagttcatct tgttgctcac cttatggcct
     3901 ttcagtggtt ggtatttatc accaagccat tattgatgcc catattgctt tggtatttta
     3961 cagaaggttt aagttttggc aaattaacac tcattcaaaa acgcatgatt acagcgctca
     4021 ctttggcatg gattggcgac attgtaaatc tttacagtca aatcaatcat tggatattta
     4081 tggcaggtgt aattattttt cttgcaatgc agattatgta tatcattatt tgcttttatc
     4141 ttattcgctt taaacaaatc aatcagctat atttcatctt cagcacctta ttatttattg
     4201 cttacggcag tgcgttgctc tggatattac tgccacatct gaatgagctt accattccgg
     4261 tattaattta tgcatctgct cttacattaa ccggaattgc ttcagcattg acaaaagatc
     4321 aactacctcg aaatgttttt tattgtttac tgacaggcac attactttac atgactactg
     4381 acagtttgga tgccatcaat aattttaaag cctctttccc ttatgcagct ttctggattg
     4441 cactcaccta tctttcagca caaatgttga ttacaattgc agtaaagaaa ataaaaacaa
     4501 tttagagtga tgaagcgtgg agtgaaaagt gatgaagtgt gaaggagcct agtgaatcag
     4561 ataatttgat aattccgaat tcatttatgt gatgaggcaa ttatttgaaa ttaggtgaaa
     4621 ttaggtgatg aaaaaaaact taggctttag catgaagaat agaagtgcaa tctctccacc
     4681 aaaaactctt ttcaattgat aattctgaac tctacattca gaattatcaa ttctctacaa
     4741 ggcctcgtcc ggttttcttt actttatctt gcttacgtag ctcttcaaca attttatcca
     4801 aaacgccatt aataaatacc ttgctctgtg gtgtactgta atctttagaa atatcaatat
     4861 attcgttgag ggtaactttt acaggtatct gatcaaattt caacacttct gtaacagcca
     4921 ttttaagcat aatcatatcg cttaatgcaa tgcggtcaac ttcccaattt ttggtgcgct
     4981 cagcaataat agaagtccac tctttatcat tcttaatcgt ttccttcaga agtctgatca
     5041 taaaatcgcg atcatcggcc tcgtctttaa ataatggtaa aattgcctgt tgatctgtag
     5101 tataaaattg tctgatagtt ttcaccacca taacatgtgc cagttcaagg ctctctgccc
     5161 accagatgtt cttttcttca ataacaccat tgatagattc cgattgctgc acttcttctt
     5221 tatacaattt cagcagccaa tccatttcta caacctctgt attggtcata gtaaactcac
     5281 gatatgccgg atgctgtctt aatttataaa aacattttcc aacagcttcc atatcggcct
     5341 gccaactgat tttatacttc ttgcaattat ctaaaaactg ctcatcgttt ttcaaccatg
     5401 atataaattc agtcttttca aaagagcgaa caaattctct tttattgact ataaactttg
     5461 atgctacatc aatgcgatac aactgttctt gctctgcaag ttcaatcaac aattgcaaaa
     5521 gggcaaggta tagttcataa aggcggctgg ttccttcaac aagttctttt tcagctttgg
     5581 tcacattata tccttcactc tgaaaataag tatataatac ctgcaatacc cgaatcctta
     5641 aatgacgtct ggctaacata aattattaaa tctaataaga acgaaaaagt ttttctttac
     5701 tataaattat ttacctccat aaatacgttt ctctgccatt tgcatagcag ctaccagtgt
     5761 agggattttc tctgttttag attttttgaa aatctctaag gtaacattgt atatatgttc
     5821 tgtttgtttt aacgcttctg cacggctgaa tttcttcaac tcgatgtaaa cattaataag
     5881 tccacctgca ttaattaaaa agtcaggcgc ccataatatg cctttgtcca ta
//
LOCUS       DAOOOL010000140         5916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1409_length_5916_cov_39.842177, whole genome shotgun sequence.
ACCESSION   DAOOOL010000140 DAOOOL010000000
VERSION     DAOOOL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_5916_cov_39.842177"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PKU82_10035"
     CDS             complement(<1..1468)
                     /locus_tag="PKU82_10035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOZ83252.1"
                     /translation="MSYFQKDIEFVKTVGPQRAAVLRKELEIHNVSDLLYHFPFRYVD
                     RSKFYTIAQLNDDLPYVQIKGVITSLTAFGNKRTTRLQAVFKDESGSMDLVWFQGITW
                     IRDSIKAGTSYVVFGKPALFNSKWSIIHPEIEELEKFTAQPFKGFQPFYYSTEKSKVK
                     GIDSKGIMRIMRMALKDLPPTIEETLSPQLIKTFNLMPRHDALIQIHFPENEDLLRKA
                     RFRLKFEELFYIQLKLLLNKQNRTATQNGRMFTEVGYLFNTFFNEHLPFELTQAQKRV
                     LKEIRKDTISGKQMNRLLQGDVGSGKTVIALFAMLLALDNKQQACLMAPTEILAAQHF
                     QTITSLLSSLPVNVELLTGSLPESKKKAVIERISSNETHILIGTHALIEDRVKFNDLG
                     IVIIDEQHRFGVEQRAKLWMRQQPPHVLVMTATPIPRTLAMTLYGDLDVSIIDELPAG
                     RKPIKTMHLFESSRLRVIGFMKEQIKLGRQIYIVFPLIE"
     gene            complement(1599..1793)
                     /locus_tag="PKU82_10040"
     CDS             complement(1599..1793)
                     /locus_tag="PKU82_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOZ83253.1"
                     /translation="MKTGKVKFYNNSKGFGFIVEDEGGRDLFVHQTGLIDRIADNDLV
                     TYEVVQGKKGLNAVNVKKQQ"
     gene            1894..3510
                     /gene="ade"
                     /locus_tag="PKU82_10045"
     CDS             1894..3510
                     /gene="ade"
                     /locus_tag="PKU82_10045"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOZ83254.1"
                     /translation="MTTSGNIIDIFNRRIFPGTVHFSKGIITEITEDNAVYDQYLLPG
                     FTDAHIHIESSMLVPYEFAKVALLHGTVATVSDPHEIANVCGLKGVEYMIENAKDAKL
                     KFNFGAPSCVPATTFETAGAVLNAEDVAQLLARPDIRYLSEMMNYPGVLFGDIEVMKK
                     IESAHRLNKVIDGHAPGLRGDDARKYIAAGISTDHECFTLEEALDKVNGGMKILIREG
                     SAARNFEALHTLITSHTAMTMLCSDDKHPDELLHGHLNQLVVRALNKGHNLFDVLQCA
                     CVNPVLHYSLSVGLLRVGDPADFIVINNPEVFLVSQTWINGECVANDGQTTLTNRSHA
                     PINNFNAWDVSVQDIEVKKNSESVKVIEALDGQLITNCIQAKPLEKDGLWVSNPENDI
                     LKIAVVNRYSKQKPVVGFIKNFGLKLGAIASTVAHDSHNIIVTGVSDADIVDAVNLLM
                     QSGGGLSAVSGTDKKVIALPVAGLMSDKSCEEIGRDYASIDSMAKQMGSALRAPFMTL
                     SFMALLVIPHFKIGDKGLFNAVKFELTSLE"
     gene            3557..3808
                     /locus_tag="PKU82_10050"
     CDS             3557..3808
                     /locus_tag="PKU82_10050"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOZ83255.1"
                     /translation="MWQEVNNSLERTFIFNDFKQAFAFMTHVAFIAEKMNHHPDWSNV
                     YNKVTIKLNTHDAGSVVTEKDHKLAAAIDVVFNQINKKV"
     gene            3805..4179
                     /locus_tag="PKU82_10055"
     CDS             3805..4179
                     /locus_tag="PKU82_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HOZ83256.1"
                     /translation="MSRFKKFFIWVVVLAIIGTALYFLAGSYTYSEGTQTGYLVKFSR
                     QGYVFKTYEGELNLGGMNAESNSVVNNLWHFSVDEDDKAAIDSLRVSEGRVIKVYYRE
                     IVRNYAWQGETPFFVYKVEKVK"
     gene            complement(4184..4753)
                     /locus_tag="PKU82_10060"
     CDS             complement(4184..4753)
                     /locus_tag="PKU82_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83257.1"
                     /translation="MQDIQPFYNWVDLYSSAEDELSPFYGRVYSEFEYSNTIYNYFIH
                     PQWDDFGSPTLYIKIIYCDYEKSFAIIELMGEWNDAINNDIMTLKRDVIENLMEHGIS
                     HFILIGENVLNFHNSDDCYYQEWFEEVNETGWIAAVNFRDHVLSEMQRANIDYYLLFG
                     GELDNLNWRTYRPSQLMAKIDSIIRHRIG"
     gene            complement(4806..5630)
                     /gene="murQ"
                     /locus_tag="PKU82_10065"
     CDS             complement(4806..5630)
                     /gene="murQ"
                     /locus_tag="PKU82_10065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOZ83258.1"
                     /translation="MNKKTESLRTTEKTSHYNHLEKMSFTVLLNNINKEDHTVPRQIK
                     KNIGAIEKLAKVITDRMKKGGRLFYIGAGTSGRLGIVDASECPPTYGIAHGKVIGLIA
                     GGDAAIRKAVEFAEDDYSGGWNDLKKYKINKKDVVVGIAASGTTPYVVGALEQCRKKN
                     IATGCIVCNSGSPVAMHADYPIEIITGPEFVTGSTRMKAGTAQKLVLNMISTAVMIKL
                     GHVKGNKMVDMQLTNNKLVSRGIRMIMDELGVPEKKATLMLKKYGSVRAAIKNHKS"
     gene            complement(5623..>5916)
                     /locus_tag="PKU82_10070"
     CDS             complement(5623..>5916)
                     /locus_tag="PKU82_10070"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HOZ83259.1"
                     /translation="KAKKYNSPIVPVYIDGRNSEWFYNLANFRKRIGIAANIEMLYLM
                     DEMYFQRGRTISITFGKPILPQSFSGTDDGKTAEKIKEHVYELKNNRQSFFHE"
BASE COUNT         1791 a         1095 c         1140 g         1890 t
ORIGIN      
        1 cttcaatcaa tggaaaaaca atataaatct gtcggccaag ttttatttgc tctttcataa
       61 atccaataac ccgaagtcgt gatgattcaa aaagatgcat ggtttttatt ggctttcttc
      121 ctgcaggaag ttcatcaatt attgaaacat caaggtcgcc ataaagtgtc atggctaaag
      181 tgcgcggaat gggtgtggct gtcatcacca gcacatgtgg aggttgttgc cgcatccaga
      241 gtttggcacg ttgctccaca ccaaaacgat gttgctcatc aatgattact atgccaaggt
      301 cgttgaattt tactctgtct tctatcaatg catgtgtacc aatgagaatg tgcgtttcgt
      361 tagatgaaat tctttctata acagctttct ttttcgattc cggtaatgag cctgtaagca
      421 attcaacatt cacgggtaaa ctactaagca atgatgttat ggtttgaaaa tgttgtgcag
      481 ctaaaatttc tgttggtgcc atcaggcagg cctgctgttt gttgtctaat gccaatagca
      541 ttgcaaaaag cgcaataacg gttttgccac tgccaacatc accttgcaac aggcggttca
      601 tttgttttcc gctgatggta tcttttctga tttctttcag tacccgtttt tgtgcctgtg
      661 tcagttcaaa aggaagatgt tcattaaaga aggtgttgaa gaggtagcca acttctgtaa
      721 acattcggcc gttttgagta gcagttctgt tttgcttgtt cagcaataat ttaagttgaa
      781 tataaaacaa ctcttcaaat ttcaaacgga agcgggcttt acgaagtaag tcctcatttt
      841 caggaaagtg aatctgaatc aatgcatcat gccgtggcat cagattaaaa gtctttatca
      901 gttgtggtga taaagtttct tcaatagtag gaggaaggtc tttaagtgcc atgcgcataa
      961 ttcgcatgat gcctttgctg tctatacctt ttactttcga tttttctgtg gagtaataaa
     1021 acggttgaaa ccctttgaaa ggttgtgcag taaatttttc aagctcttct atttcaggat
     1081 ggataatact ccacttgctg ttaaataaag caggcttgcc aaaaacaaca taactcgttc
     1141 cggcttttat ggaatcacga atccatgtta ttccctgaaa ccaaaccaaa tccatgctac
     1201 cgctttcatc tttaaaaaca gcttgcaggc gtgtagttcg tttgttgcca aaagctgtta
     1261 gtgaagtaat cacaccttta atctgcacat aaggcaggtc gtcattcagt tgtgcaatgg
     1321 tgtaaaattt tgaacggtca acatagcgaa acggaaagtg atacaaaagg tcagaaacat
     1381 tatgaatctc taactcctta cgaagcacag ctgcacgttg cggaccaact gtctttacaa
     1441 actctatgtc tttctgaaaa taactcactc ctgcctctga aaaaattaaa ttaattacat
     1501 ggctgaaaaa tacgggtaaa tgtgcaaaaa ataaaatccc cggctgaatt acaaccgggg
     1561 aataacatta aaaaattaca agtaaatcta tgtgattatt actgttgctt tttaacattt
     1621 acggcattta agcctttttt gccttgaact acttcatagg taacaaggtc attgtccgcg
     1681 atgcggtcaa ttaatcctgt ctgatgaaca aataggtcac gaccaccttc atcttcaacg
     1741 ataaatccaa atcctttgga gttgttgtag aattttactt ttccggtttt cattaaataa
     1801 agaattaaat ttgtgaatta atgctgcaaa tgtaaactaa tttgaatatc atttatttaa
     1861 aatacaaaat aatttattgt cggaacctga cgtatgacca caagcggaaa tattattgac
     1921 atttttaacc gaaggatttt ccccggaact gttcacttta gcaagggaat aattacagaa
     1981 attacagagg acaatgctgt ttatgaccaa tatttactac ccggttttac cgatgcccat
     2041 atacatattg aaagcagcat gcttgtgcct tatgaatttg ctaaagtagc cctgcttcat
     2101 ggcaccgtag caactgttag cgacccgcat gaaattgcca atgtttgtgg cctcaaaggt
     2161 gtggaatata tgattgagaa tgcaaaagat gccaagttga aatttaattt tggtgcacca
     2221 tcatgcgtgc ctgccaccac ttttgaaaca gcaggtgcag tgcttaatgc cgaagatgtg
     2281 gcacaacttc ttgcacgacc cgatattcgc tatctgagcg aaatgatgaa ctaccccggt
     2341 gtactttttg gcgatataga ggtgatgaaa aaaattgagt cggctcacag actgaataaa
     2401 gtgattgatg gtcatgcacc cggtttaaga ggtgatgatg cccgaaagta tattgctgca
     2461 ggcatatcta ccgaccatga atgttttacg cttgaagaag cattggataa ggtaaatgga
     2521 ggaatgaaaa ttctgatacg tgaaggctct gctgcacgga attttgaagc cctgcatacg
     2581 ctcattactt cacatacagc aatgactatg ttgtgtagcg atgataaaca tcctgatgaa
     2641 ttgcttcatg gacacctgaa tcagttagtt gtccgcgctc tcaataaagg acacaatctt
     2701 tttgatgttt tgcaatgtgc ttgtgttaat cctgtgttgc attatagttt atccgtagga
     2761 ttacttcgtg ttggtgatcc ggcagatttt atcgttatca ataacccgga agtgttttta
     2821 gtctcgcaaa catggataaa tggagaatgt gttgccaatg atggccaaac aacattgaca
     2881 aaccgtagtc atgctccaat aaataatttt aatgcatggg atgtttcggt tcaggatatt
     2941 gaagtgaaaa aaaattccga atcggttaaa gttatagaag cattggatgg gcagttaatt
     3001 accaactgca ttcaggcaaa gccattagaa aaagacggac tgtgggttag caatccggaa
     3061 aacgatattc ttaaaattgc agtcgttaac cgttatagca aacaaaagcc tgttgtagga
     3121 tttattaaaa attttggatt aaagttagga gctattgcat caaccgttgc acatgactcg
     3181 cacaatataa ttgtcaccgg tgttagtgat gctgatattg ttgatgctgt aaatttactt
     3241 atgcaatcgg gtggaggact gagtgctgta tcaggaacag acaaaaaagt tatagccttg
     3301 cctgtagcag gactgatgag tgataaaagt tgcgaagaaa ttggtcgcga ttatgcaagt
     3361 attgatagta tggcaaaaca aatgggatct gcactaagag caccttttat gacactatca
     3421 tttatggctt tattggtaat tccacatttt aaaattggcg ataaaggatt attcaatgct
     3481 gtaaagtttg aactaacttc actggaataa tttatttgtg tttagcaatg aagtactact
     3541 tttacaaaaa aaatgtatgt ggcaagaagt aaataattca ttggaacgaa catttatttt
     3601 taatgatttt aaacaagcat ttgcatttat gacacatgta gcttttattg ctgagaaaat
     3661 gaatcatcat cccgactgga gcaatgttta taataaagtc actattaagt taaacacaca
     3721 cgatgcagga agtgtggtta cagaaaaaga tcataaactt gcagcagcaa tagatgttgt
     3781 gttcaatcaa ataaataaaa aagtatgagc cgttttaaaa agttttttat atgggtagtt
     3841 gttcttgcca taatcggaac agcattgtat tttcttgcag gaagttatac ctattccgaa
     3901 ggaacacaaa caggctactt agtaaaattt tcacgtcagg gttatgtttt taaaacttat
     3961 gaaggtgaac taaatcttgg tggtatgaat gccgaaagca attctgttgt aaataacctc
     4021 tggcattttt ctgttgacga agatgataag gctgctattg acagtcttcg tgtcagcgaa
     4081 gggcgtgtga ttaaagttta ttaccgtgag attgtacgca attatgcatg gcagggcgag
     4141 acaccttttt ttgtttacaa agtagaaaaa gtaaaatagg tttttatcct atacgatgcc
     4201 tgataataga atctatttta gccattagtt gcgaaggcct gtaggtgcgc cagttaagat
     4261 tatcaagttc accgccaaac aataaatagt aatcaatatt ggcacgttgc atttcagaca
     4321 aaacatgatc tctgaaattg acagcagcaa tccatcctgt ttcattcact tcttcaaacc
     4381 attcctgata gtaacaatcg tctgaattgt gaaagttgag tacgttttca ccaataagaa
     4441 taaaatggct tataccatgc tccatcagat tttctataac atctcttttt aaggtcatga
     4501 tgtcattatt gatggcatcg ttccattcgc ccatcaattc aataatggca aatgactttt
     4561 catagtcgca ataaataatt tttatataaa gcgttggcga gccaaagtca tcccactgcg
     4621 gatggataaa atagttgtaa atggtattgc tgtattcaaa ctcactgtaa acccttccat
     4681 aaaaaggact caactcatct tctgcagatg agtatagatc tacccagttg taaaaaggct
     4741 gtatatcctg cattgtttga taataaaatg caaaattaca acagacaaat tcaaactaca
     4801 acagtttatg atttatgatt ttttattgct gcccgcacgc tgccgtattt tttcagcatc
     4861 agagtagctt ttttttcggg tacgccaagt tcatccatta tcattctgat gccacggctg
     4921 actaatttat tattggtaag ctgcatgtca accattttat tgcctttaac atgaccaagt
     4981 tttatcatca ctgccgtact tatcatattc agaactagct tctgtgcagt tcctgctttc
     5041 atgcgagtgc tgccggttac aaattcaggc cctgtaataa tttctatagg ataatcagca
     5101 tgcatggcaa caggagagcc gctattacaa acaatgcatc ctgtagcaat atttttcttg
     5161 cggcattgtt caagagcacc tactacatag ggcgtagttc ccgaagctgc aatgcctact
     5221 acaacatctt ttttatttat tttatatttt ttaagatcat tccatccccc tgaataatca
     5281 tcttctgcaa actcaacagc cttgcgaatg gcagcatcac ctccggcaat aagtccaata
     5341 acttttccat gcgcaatacc ataggttggt ggacactcag aagcatcaac aattcctaat
     5401 ctgccactgg tgccggcacc aatataaaat aaacgtccac cttttttcat tctgtcagta
     5461 atgacttttg ctagtttttc aattgcccca atattttttt tgatctgtcg cggaacagta
     5521 tggtcttcct tattgatgtt attcagcaat actgtaaaac tcatcttttc gagatgattg
     5581 taatgcgaag ttttttcagt agtccgcagg ctttctgttt tcttattcat gaaaaaaaga
     5641 ttgtcggtta ttttttagct cataaacatg ctccttgatt ttctctgcag tttttccgtc
     5701 atcagttcct gaaaagcttt gtgggagaat aggttttccg aatgtgatgg agatggttct
     5761 gccacgctga aaatacattt catccatcag gtaaagcatt tcaatgtttg ctgcaatacc
     5821 aattcgcttt ctgaagttgg ctaaattgta gaaccattct gagtttcgtc catcaatata
     5881 aaccggcaca ataggactgt tgtatttttt tgcttt
//
LOCUS       DAOOOL010000141         5841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1440_length_5841_cov_54.837885, whole genome shotgun sequence.
ACCESSION   DAOOOL010000141 DAOOOL010000000
VERSION     DAOOOL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_5841_cov_54.837885"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKU82_10075"
     CDS             <1..361
                     /locus_tag="PKU82_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83260.1"
                     /translation="LSYSLDSLFRTSNGKSDFVIRMLKVFISSNQSLIEKSIHFAKES
                     DFQQSAAAVHRMIPGFRQLDLDTFASILKNIEWLMLEGTEKNAATELLQQCAEQFEKL
                     ISAMQADIDRLEKEATT"
     gene            complement(354..1823)
                     /locus_tag="PKU82_10080"
     CDS             complement(354..1823)
                     /locus_tag="PKU82_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83261.1"
                     /translation="MDILNQLITGLNKEEVRFFKMYINRVEKTDNRKDVQLFDYIRKS
                     GDKFSDAIIFKKLYQPQEKNSYYRLKNRLIREVNKSLTLQHFDDDMVVYIFRLLALVK
                     FYLNKNALKPAYFFLRKAEQKANDTESLELLDIIYGEYIRLSREMTSINPEIYIHRRK
                     QNSEKLNQLRVIDDILAVVTYRLKITQNLSSKDDTVISLLQKTTDDFIGDPKIIKSPL
                     FRFRIYNAISQLLLQSHQYVLLEDYLLKTLKIFNQEKLFTKNNHETKLQLLVYLVNSL
                     YKNNKLKKSLEYINMLHQEMKAYNHLFYDKFLFYYYNALVINYSKTDKQKEIVTLQEM
                     EQNEKINANLFYRQYILLNFALAYFDQKEFSMSAKSFTRLQMIPDYKETDESLRMKVA
                     VAELMTRYELNDYNTFEYKLKQFNKDFRSGLNKPENEPEKIMSEILKQMMSVSNRKNR
                     VIELRIAQLKTLSKDEDKSELINYSDWLQEKKLLKHKIR"
     gene            1923..2621
                     /locus_tag="PKU82_10085"
     CDS             1923..2621
                     /locus_tag="PKU82_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83262.1"
                     /translation="MKRVQIIVLFFSLLFCGTASAQIDSLGVIAISPQYPQCPDSAFM
                     QQPYFNIQVSIKNYNANSTFTGGLMLKLQSDTLPEDSIVLNAGTVYTIQPNGSINITI
                     QNPYFFNVASYKLGSNVVVVWPVSTQGGTVVKYNPYLACVLFLPFVGINEYDRDKLSV
                     MPNPSNKFIDLNIPSNIGLKKVRIFNPTGEEVRNYSVDLSNRITVEQLASGIYFLEVI
                     TTDDHVYNTKFIKN"
     gene            2651..3322
                     /gene="rsmI"
                     /locus_tag="PKU82_10090"
     CDS             2651..3322
                     /gene="rsmI"
                     /locus_tag="PKU82_10090"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOZ83263.1"
                     /translation="MLYIVPTPVGNLEDITLRALNILKSCDIILVEDTRNASVLLNHF
                     GISKKVFTHHQHNEHAAVNELIRLLKDEKKTIALISDAGTPGISDPGFLLTRACIEHE
                     IELTCLPGATAFVPALVMSGIPCNSFVFEGFLPAKKGRQTRLKLLQEETRTMVFYESP
                     HKLIKSLHDFITNFGADRKVCVVREISKMFEEKKRGTLAEVCEYYTNHPPKGEIVIVV
                     AGKEE"
     gene            complement(3294..3923)
                     /locus_tag="PKU82_10095"
     CDS             complement(3294..3923)
                     /locus_tag="PKU82_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOZ83264.1"
                     /translation="MLFVQPSYLIRLFYSGLLWRMPTRQPIVYLTFDDGPEPEATPFV
                     LSQLKKWNALATFFCIGNNIEKHQDLFSRVVSAGHQIGNHTQHHLNGWKNNLNTYLAD
                     VEQCSTHISSGLFRPPYGKITQKQIRSLRSQWQIVMWDVLSYDFETNVSPEKCIQNVM
                     QHVRPGSIIVFHDSKKAFKNLSETLPVILEKLSAEGYSFKAIPPYRPLQ"
     gene            complement(4032..5174)
                     /locus_tag="PKU82_10100"
     CDS             complement(4032..5174)
                     /locus_tag="PKU82_10100"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOZ83265.1"
                     /translation="MHNKVQLKFSLALLLTCFSVSDTFSQCCAGGSGSPIAGGTSQGV
                     LQEKQVEISTNFQFISTDKFYKKNKVAPDSVRTFDGFNSTYQYFRLAYGITKSLTFSV
                     ESGYYYQKKETGLHKDAATTYNAKGFGDLILFPRYDIINWTEEKHRTEVTVGLGFKIP
                     LGSYNDSTGNVEPFSGYTYYVTNPTSVQLSSGAHDIIFYTFLYRGFTEKNFRVFANAL
                     YIKKGYNPNGEKLGDFASVGIFAGKTFFNNLGVTLQARYEWMDRMKVNESISLFGKPS
                     NYFPKATGYKKIFISPQLSYTKGKFTVYGSYDIPVYQYLNTSEHYTQMGSQHQATLGV
                     TFRFMALNSYLKEKSGVTYVCPMHPNVISDKPGKCPDCGMDLVKKK"
     gene            complement(5223..>5841)
                     /locus_tag="PKU82_10105"
     CDS             complement(5223..>5841)
                     /locus_tag="PKU82_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83266.1"
                     /translation="PCASCTGPSLTTYNVVQSFQASNPNKVFMYTCDDFANTNCTSLN
                     SWANGIGVMQQSWSLRFSNAAINMTHYGSPGMPKIVVLGPNHAVFYNSNNTVNATNLQ
                     NAINNALATTGLNESNLPASSLSVLPNPAQNEAKIKLNLLNKADVILECYNLEGKKVQ
                     EIFSGNLTKGEHTIPVNLEQFTDGMYLIKFSDGTQTQFINLIKSN"
BASE COUNT         1835 a          994 c         1014 g         1998 t
ORIGIN      
        1 gttaagttat tctctcgata gcttatttag gacatcaaat ggtaaaagcg attttgttat
       61 cagaatgttg aaggttttta tttcaagtaa tcaaagtttg atcgaaaaga gtattcattt
      121 tgctaaggaa tcagattttc agcaatcggc agcggccgtt cacagaatga ttccgggctt
      181 cagacaactc gatttagata cctttgcatc aatcttaaaa aatatagaat ggctgatgct
      241 tgaggggact gaaaaaaatg ctgcaactga attgttacaa caatgtgcag aacagtttga
      301 aaagcttatc tctgccatgc aggcagatat tgaccgcctc gaaaaggaag caactacctg
      361 attttatgtt tcaataattt tttttcctgc agccaatcgg aataatttat caattcactt
      421 ttgtcttcat ctttcgatag cgttttgagt tgtgctatcc gcaattcaat tactctgttt
      481 tttctgttac ttacagacat catttgtttt aatatctcac tcattatttt ttcaggttcg
      541 ttctcaggtt tatttaagcc acttctaaag tctttattga attgtttgag tttgtattca
      601 aacgtgttat agtcgtttaa ctcatagcgt gtcataagtt ctgcaacggc aactttcatt
      661 cgcagcgatt catcagtttc tttatagtca ggtatcattt gtaatcgggt aaaacttttt
      721 gctgacatac taaattcttt ctgatcaaaa tatgccaaag caaaattcaa cagaatatat
      781 tgacgataaa ataagttagc attaatcttt tcattttgtt ccatctcttg caaggtcact
      841 atttcttttt gtttatcagt tttagaatag ttaatcacaa gcgcattgta gtaataaaat
      901 aaaaatttat catagaaaag atgattatat gctttcatct cctgatgtaa catgtttatg
      961 tattcaagcg acttttttag cttgttgttt ttatagagac tattaaccaa atagaccaac
     1021 agttgaagtt tggtttcatg attgttttta gtgaaaagtt tttcctgatt aaatattttt
     1081 agtgttttta acaggtaatc ttctaacaaa acatattgat gcgattgtaa aagcaactga
     1141 ctaattgcat tataaatcct gaaacggaag agcggacttt ttataatttt cggatcaccg
     1201 ataaagtcat cagtagtttt ctgaagcagt gaaattaccg tatcgtcttt tgatgataag
     1261 ttttgagtaa tttttaaacg ataggtaact actgccagta tatcatcaat gacacggagt
     1321 tgattgagct tctctgaatt ttgctttctg cgatgaatgt aaatctcagg attaatactt
     1381 gtcatctcgc gtgaaagcct gatgtattca ccataaatga tatcaagcag ttctaaactt
     1441 tctgtatcat tggccttctg ttctgcttta cggagaaaaa aataggccgg ttttaaagca
     1501 tttttattta gataaaattt taccaatgcc agcaacctga aaatatatac aaccatgtcg
     1561 tcatcaaaat gctgtagggt cagactttta tttacctccc tgatgagtct gttttttaag
     1621 cggtagtaag aatttttttc ttgtggttga tataattttt taaagataat tgcatctgaa
     1681 aatttatctc ctgatttacg gatgtaatca aacaattgta catctttacg gttgtcagtt
     1741 ttctcaacac gattgatgta catcttaaaa aaacgtactt cttctttatt aagtccggtt
     1801 atcagttgat ttaaaatatc cattatagcc tttttgggca ggtaagataa tacaaaaata
     1861 agtatcttgg attaaaaaac aatttatact tttattaagt ctttagttta aaatacttca
     1921 tcatgaaaag ggttcagata atagtgttgt ttttcagttt gttgttttgt ggcactgctt
     1981 cagcacaaat agattcatta ggcgttattg ctatttctcc gcagtaccct caatgtcctg
     2041 attcggcctt tatgcaacag ccttatttta atattcaggt ttccattaag aattataatg
     2101 ccaattccac ttttacaggt ggattgatgt tgaaacttca gagcgacaca cttcctgaag
     2161 attctatagt tcttaatgcc ggaacggtat ataccataca acctaatggc agtataaaca
     2221 ttactataca aaatccttat tttttcaatg tggctagtta caaattaggc agtaatgttg
     2281 tagtggtttg gcctgtaagt acacaaggcg gcacggttgt caagtataat ccttatttag
     2341 cttgtgtatt atttctgcca tttgttggta taaatgaata tgacagagat aaactaagtg
     2401 ttatgcctaa cccttcaaac aagtttattg acctgaatat accatcaaat attggtctta
     2461 aaaaggtaag aatttttaat ccaaccggtg aagaagtaag aaattattct gttgacttat
     2521 caaatcggat aacagtggaa caactggcaa gcgggattta ttttttagag gtcatcacta
     2581 ccgatgatca tgtttataac acaaagttta ttaaaaactg aaataaagtt ctattggtta
     2641 cttttgatga atgttataca tcgttcccac gcctgtaggt aacctggaag atattacact
     2701 tagggcatta aatattctaa agtcatgcga cattattctt gttgaagaca cacgcaatgc
     2761 atcagtactt ctcaatcatt tcggaatttc aaaaaaagtt tttacacatc atcagcacaa
     2821 tgaacatgca gcagtcaatg aattaatacg tttacttaaa gacgaaaaaa agacaattgc
     2881 cttaatcagt gatgccggga cacctggtat atcagaccct gggtttttac ttacacgtgc
     2941 ctgtattgaa catgaaatag aacttacttg tctaccgggt gccacagctt ttgttccggc
     3001 cttggtaatg tcaggaattc cttgcaacag ttttgttttt gaaggctttt taccagctaa
     3061 gaaaggacga caaactcgcc ttaaactcct tcaggaagaa acgcggacaa tggtttttta
     3121 cgaatcgcca cataagctta ttaaatccct acatgatttt ataaccaact ttggtgctga
     3181 cagaaaggtt tgtgtggtgc gtgaaatttc aaaaatgttt gaagaaaaaa aacgaggtac
     3241 tttagcagag gtctgtgaat attataccaa ccatccacct aaaggcgaaa ttgttattgt
     3301 agtggccggt aaggaggaat agctttaaaa gaataacctt ctgctgaaag cttttccaaa
     3361 ataacaggta gtgtttcact taaattttta aatgcttttt tactatcgtg aaaaactatg
     3421 atagaccctg gacgcacatg ttgcatcaca ttctgaatac atttttcagg cgatacattt
     3481 gtttcaaagt cgtaagacaa aacatcccac ataacaattt gccattgtga acgcagtgaa
     3541 cgaatttgtt tttgtgttat tttaccatat gggggtctga ataaaccgga tgaaatatgt
     3601 gtgctgcatt gttcaacatc tgccaaataa gtattgaggt tattcttcca tccattcaaa
     3661 tgatgctgtg tatgattccc tatctgatgc cctgcagaaa caacccgact aaacagatcc
     3721 tgatgtttct cgatattgtt tccaatacaa aagaatgttg caagagcatt ccatttcttt
     3781 aactgcgaca atacgaaagg tgttgcctca ggctcaggac catcatcaaa tgtcaggtaa
     3841 actattggtt gtcgcgttgg catacgccat aacaatcctg aataaaaaag tctgattaaa
     3901 tatgatggtt gaacaaataa catttatgaa tactattcct tctttttgcc aaaaataaac
     3961 aacctgatca tgtctttgtt ggatgaatag ttatatgtga aagtataaaa tcagaataat
     4021 aaaaagtaac tctatttttt cttaaccaaa tccattccgc aatcgggaca cttaccgggt
     4081 ttatctgaaa taacattcgg atgcatgggg catacatagg taactcctga tttttctttt
     4141 agataactat ttaaagccat aaagcggaaa gtaacaccaa gtgttgcctg atgttgtgaa
     4201 cccatttgtg tgtagtgttc tgatgtattt aagtactgat aaacgggaat gtcataagaa
     4261 ccataaaccg tgaacttacc tttggtatag ctgagttgtg gtgatataaa aatcttttta
     4321 taacccgtag ctttgggaaa ataattcgat ggttttccaa acagagaaat agattcatta
     4381 actttcattc tgtccatcca ttcatatcgt gcctgcaagg ttacacctaa gttgttgaag
     4441 aatgttttac ctgcaaaaat tcctacactg gcaaagtcac ccaatttttc tccattaggg
     4501 ttatatcctt ttttaatgta caaggcatta gcaaaaactc tgaaattttt ttctgtaaat
     4561 cctctgtaaa gaaaagtgta aaaaataata tcgtgtgcgc ctgatgaaag ttgtaccgat
     4621 gtggggttgg taacataata ggtgtatccc gaaaatggct ctacattgcc tgttgaatca
     4681 ttatagcttc cgaggggaat tttaaatcct aaaccaacgg taacttcagt acgatgcttt
     4741 tcttccgtcc agttaataat atcataacgc ggaaaaagaa ttaaatcacc aaaacctttt
     4801 gcattgtatg ttgtggctgc atctttatgc agacctgttt ctttcttttg ataataatag
     4861 ccgctctcaa cagaaaatgt taggcttttt gtaataccat aagccaatct gaaatattga
     4921 taggtactat taaaaccatc aaatgttcgt actgaatcag gtgcaacttt atttttttta
     4981 taaaatttat cggtgctgat aaattgaaaa ttagtactta tttcaacttg tttttcctga
     5041 agaacacctt gtgaagttcc tccggcaatt gggctgccac ttccacctgc acaacattgc
     5101 gaaaaggtat cagaaacgga gaagcaagtt aataacagag ccaaagaaaa tttcagttga
     5161 actttattat gcatagatat ttaatttaca aaatttactc caactgtaaa actattattc
     5221 tgttagttgg attttatcag attaataaac tgtgtttgtg taccgtcaga aaatttgata
     5281 agatacatgc catcagtaaa ttgttcaaga tttacaggaa tagtgtgttc tcctttagtc
     5341 aggtttcccg aaaaaatttc ctgtactttt ttcccttcaa gattatagca ctccagaatt
     5401 acatcagctt tattcagaag gttaagttta atttttgctt cgttttgtgc tgggtttggc
     5461 agtacagata atgatgatgc aggtaaattg ctttcattta aacctgttgt tgccaaagca
     5521 ttgttaatag cattttgcag gttagttgca ttaacagtat tgttcgaatt ataaaataca
     5581 gcatgattag gtcctaaaac tacaatcttt ggcattcccg gtgaaccata atgcgtcatg
     5641 ttaattgctg cattcgaaaa tcgtaacgac cagctttgtt gcataacacc aattccgttt
     5701 gcccaactgt ttaaagaagt acaattggtg tttgcaaaat catcacaggt gtacataaaa
     5761 actttattag gattggaagc ctgaaaactc tgaacaacat tataggttgt taaagaaggt
     5821 ccggtacaag atgcacaagg c
//
LOCUS       DAOOOL010000142         5835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1442_length_5835_cov_112.752422, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000142 DAOOOL010000000
VERSION     DAOOOL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_5835_cov_112.752422"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKU82_10110"
     CDS             <1..735
                     /locus_tag="PKU82_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOZ83267.1"
                     /translation="TSGYPLNSSVFEYNPINNVWFSKSGFPGNPRQSATSFVVNDTAY
                     MVSGYLTSSPSNELWRYNQLSDTWTMMQSLPNNPRYGAVGFELSGLGVITTGGNGTYY
                     FTDGYAYSPPINQWLNIPQFTGAIRRHSASFTINNKGYVSCGISATGSTLNDLWELSD
                     VTALPEAASTQPKVYYNQSYSTLTVTGLANKKSTLKIFSLSGQMVYSQQCYSDMEQIP
                     LKLNKGIYSYNIEGIGKMMNGKFEKL"
     gene            759..2375
                     /locus_tag="PKU82_10115"
     CDS             759..2375
                     /locus_tag="PKU82_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83268.1"
                     /translation="MLFILLSTVNAEGQMWQWAKSDGSLNNDAAISVSTDYAGNVFVA
                     GIFRDSIFSGANKLYSYGLDDIYVVKYSPNGNILWTKCFGGTGNDGYGLYIKADSIGN
                     CYVTGAYSYSAQFDLITLNSGFVNNMFLVRMNPTGYILFANDFQGTNGSVGYALEVDR
                     NQNCYLTGYFYDQLFAAPNTVYSNGSHDIFLAKIDGLGNVLWSRRAGGSSYDSGGGVA
                     LDDFGNIYITGYFSSTADFDIATLISSGGDDAFVAKYDNSGNFKWVSRTFGSGVYDIG
                     VKIRIQQDKICVVGQFEDTMTIGLQTLISYGGMDGFITLLDSSGNFLWAKQLGSVGND
                     RFSDVAFVSNSEIVASAWINQTAFLNNTLINVFNKSGLVSWFDYNGNIISYKQASNIA
                     KNSFNALCTNISNEILVAGSMEDTLQLDSIKLASVGLADMIIGKIVKRETAVTEVQDN
                     EFLVQNNLFSDETRPSIIFSEQVQIKSVEIFDMNGNCVVKYFPQRKLDVGNKFTINNP
                     VMSQLSQGVYLVRITTGISDKIVLIKIIKV"
     gene            2409..2741
                     /locus_tag="PKU82_10120"
     CDS             2409..2741
                     /locus_tag="PKU82_10120"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HOZ83269.1"
                     /translation="MRIIGFIFSILFIVSCTNKPAEVKTPAPLINNVTVQTYANDSTL
                     GGYGYDILVDGKIMVHQPNIPAVMGNRGFATEEDASKTATLVSFKLKNNIMPPSVTLE
                     ELDSIGIK"
     gene            2761..3297
                     /locus_tag="PKU82_10125"
     CDS             2761..3297
                     /locus_tag="PKU82_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83270.1"
                     /translation="MEELGIGSRIRHPEFGIGVVINVKPRTYITVFTERGKIEVAKTY
                     QNLEVIDAQPPVGDPVSFADMERVFTNIIKKYSDVQETVPLHGRWIGGKVVLHPNNPS
                     QKPKEIPMDVFFNKIIMIRDRLRVMEQRINAHDKLTEEDKINLQQYLTRIYGSLTTFN
                     VLFKDEEDVFVGEKGNKE"
     gene            3297..3677
                     /locus_tag="PKU82_10130"
     CDS             3297..3677
                     /locus_tag="PKU82_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HOZ83271.1"
                     /translation="MNKKILIFAALSGLCAVALGAFGAHKLKELLSPQMLSAFETGVR
                     YQFYHTLALLFVALAANENKMLNRAATFFMTGIILFSGSIYGLALSSISGNTWIWLGP
                     VTPIGGLCFMTGWIFLIIYAFKKQ"
     gene            3677..5170
                     /locus_tag="PKU82_10135"
     CDS             3677..5170
                     /locus_tag="PKU82_10135"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOZ83272.1"
                     /translation="MSELYQQYVTHLQKIADINYSSAVLQWDQEVYMPVKGAHYRAQQ
                     LSTLAGYAHELITDKRYEEIIEKLHDDTSKLSPVQCANVLESYRIFKQQKKYTTEFVV
                     ALNQCISKSFNAWQRAKQENNFKLFEPHLQELIKFKRQESELLGYEQHPYDAHLNLYE
                     TGLKTSFLETIFNQVRKKLVPYIQQLVQKPQPDDNFLFKSYNKDKQWHFGLDVLKQMH
                     FDFESGRQDISTHPFTTSFSANDVRVTTRISEHDLFEMIGSCIHEGGHALYEQGLPAD
                     DYGLPSGEYLSLSIHESQSRLWENHVGKSQYFWNHNFKTLRNIFPEQLHDVSERSFYK
                     AINRIKTSLIRTNADELTYHLHVLIRFEIEKDILSGALQAAQLPEVWNEKYKSYLNID
                     VPSDAKGVLQDVHWSHGSFGYFPTYSLGSFYASQFFHQANKEIPALMHHIQNGDTMPL
                     LMWLRSNIHRHGRTILADELCKNVTGEVLNFNYFFNYAKTKYDELYA"
     gene            5154..5606
                     /locus_tag="PKU82_10140"
     CDS             5154..5606
                     /locus_tag="PKU82_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83273.1"
                     /translation="MNYMLNRMNCCALVLIVAMAGSCKTNKNTTAETPKQEPAATNPR
                     VDLPDSMYRVIVSFISIGAGTDGAAKEKLEQVVQQYQTEVGMPVAHEEIAWGREGEVD
                     YCFRLLGQDKKSKDSFVQHLRDTFKGNSLVQITENTPALHQARPSFNK"
BASE COUNT         1866 a          950 c         1204 g         1815 t
ORIGIN      
        1 acctctggat atcctctgaa tagttctgta tttgaatata atccaataaa taatgtttgg
       61 ttttcaaaat ccgggtttcc gggtaaccca cgtcaaagtg caacttcctt tgtagtcaat
      121 gatactgctt atatggtgtc tggttatctg acttcaagtc cttcaaatga attatggcga
      181 tataatcaac tttcagatac ttggacaatg atgcagtcat taccaaataa tccaagatac
      241 ggtgctgttg gttttgagtt gtcaggttta ggtgttatca caactggagg aaacggtact
      301 tattatttta ccgatggata tgcatactcg cctccaataa atcaatggtt aaatattccg
      361 cagtttactg gagcaataag aagacatagt gcttcattta ctattaataa taaaggatat
      421 gtttcatgtg ggatttctgc aactggaagt actttgaacg acctctggga attatctgat
      481 gtaacggcat tgcccgaagc ggcaagcact caacctaaag tttattataa tcaatcatac
      541 tcaacactaa cggttacggg attggcgaat aagaaatcaa cattaaaaat attttctctg
      601 tcggggcaga tggtttattc gcaacaatgc tattcagata tggagcaaat accgctaaaa
      661 ctcaataaag gaatttattc ttacaacatt gaaggaattg gtaaaatgat gaatgggaaa
      721 tttgaaaagt tatgagaatt aagaatataa tttccgcaat gttatttatt ttactgagta
      781 cagttaatgc agaggggcaa atgtggcaat gggctaaatc agatggatcg ctaaataatg
      841 atgctgctat ttctgtctca acagactatg caggtaatgt ttttgttgcc ggaattttca
      901 gagattcaat ttttagtgga gcaaataaat tgtatagtta tggtcttgat gatatttatg
      961 tagttaagta tagtccgaat ggaaacattc tctggactaa atgttttggt ggaactggaa
     1021 atgatggtta tggtctatat attaaggctg attcaattgg aaattgttat gtaacgggtg
     1081 cttacagtta ttcagcacaa tttgatttaa tcacattgaa ttcggggttt gtaaataaca
     1141 tgtttttagt aaggatgaac cctaccggat atattttgtt tgcaaatgat tttcaaggaa
     1201 caaatggcag tgttggttat gcattggagg ttgacaggaa tcagaactgt tacctaacag
     1261 gctattttta tgatcaatta tttgcagccc cgaatacagt atattcaaat ggtagtcatg
     1321 atatttttct tgccaaaatt gatggcttag gaaatgttct ttggtctagg cgggcaggag
     1381 gtagcagcta cgattcagga ggaggtgttg cattagacga tttcggaaat atttatatta
     1441 ccggatattt ctcatcaact gcagattttg atattgcaac attaattagt agtggaggcg
     1501 atgatgcttt cgtagcaaaa tatgacaata gcggtaattt caaatgggtt agcagaactt
     1561 ttggaagtgg agtttatgat ataggtgtaa agattagaat ccaacaagat aaaatttgcg
     1621 ttgttggaca gtttgaagac acaatgacaa ttggactaca aacattgatt agttatggag
     1681 gcatggatgg atttataaca ctactggata gttccggtaa ttttttatgg gctaagcaat
     1741 taggtagtgt aggaaatgat cgtttttctg atgttgcctt tgtttccaat agtgaaatag
     1801 ttgcatcggc ctggattaat caaactgcat ttttaaataa tactttaatc aatgtattca
     1861 ataaaagtgg gttggtaagt tggtttgatt ataatggtaa cataatttct tataaacaag
     1921 catccaatat tgcaaaaaat tcattcaatg ctttatgtac taatattagc aatgaaattt
     1981 tagttgcagg ttctatggaa gatacactgc aattggatag tattaaatta gcttctgttg
     2041 gattagctga tatgataatt ggtaaaatag tgaaaagaga aacagcagtt actgaagtac
     2101 aggacaatga atttttagtt cagaataatt tattttcaga tgaaactcga ccatcaatta
     2161 ttttttcgga gcaagttcag attaaatctg tagagatatt tgatatgaac ggcaattgtg
     2221 tggtaaaata ttttcctcaa agaaaacttg atgtagggaa taaattcaca attaacaatc
     2281 ctgtaatgtc acagctttca caaggtgttt atttagtcag aattacaacc ggtatttccg
     2341 acaaaattgt gttaatcaaa attattaaag tttgagttct tttatataaa accatttatt
     2401 tttgagacat gagaataata ggttttatct tttcgatact attcattgta tcttgcacta
     2461 ataagccggc tgaagtaaag actccagccc ccttaatcaa caatgtcact gttcagactt
     2521 atgcaaacga tagcacatta ggcggctatg gctacgatat tttagttgat ggaaaaatta
     2581 tggtacatca acctaacatc cctgctgtaa tgggtaatcg tggctttgcc acagaagagg
     2641 atgcctcaaa aacagcaaca cttgtatctt ttaaactaaa aaacaatatc atgccgccct
     2701 ctgttactct tgaagagtta gacagtatag gcattaaata atacttacta aatattttat
     2761 atggaagaat taggaatagg ttcacgcatc agacatcccg aatttggcat tggtgtggtc
     2821 atcaatgtaa aaccacgaac ctatattacg gtatttaccg agcgtggtaa aatagaagtt
     2881 gctaaaacat atcaaaacct ggaagtaata gatgcgcaac cacccgtggg cgaccctgtg
     2941 agttttgctg acatggaacg tgtgtttaca aacatcatta aaaaatattc agacgtgcag
     3001 gagaccgtgc ctttgcatgg tcgctggatt ggaggtaagg ttgtgttgca tcccaacaat
     3061 ccttcacaaa agcctaaaga aattcctatg gatgttttct ttaataaaat aattatgatt
     3121 cgcgaccgac tgcgcgttat ggaacagcgc atcaacgcac atgataaatt aaccgaagaa
     3181 gataaaatca accttcagca atatctgaca cgtatttatg gcagcctcac cacctttaat
     3241 gttttgttta aagatgagga agatgttttt gtaggcgaga aaggaaataa ggaataatga
     3301 ataagaagat tttgattttt gccgccctat caggcctttg cgcagtggca ttgggtgcct
     3361 ttggtgcaca taagcttaaa gagttgttgt cgccacagat gttgtcggcc tttgaaacgg
     3421 gtgtgcgcta tcagttttat cacacacttg ccttattgtt tgttgctctt gccgcaaatg
     3481 aaaataaaat gcttaaccgt gctgcaactt tttttatgac aggtataata ctttttagtg
     3541 gctctattta tggattggca ttgtcatcta ttagtggcaa cacctggata tggcttggac
     3601 ctgttactcc tataggcggg ctttgtttta tgacgggatg gattttcttg attatttatg
     3661 catttaaaaa acaataatga gtgaactata tcaacaatat gtaacccacc ttcagaagat
     3721 tgcagacatc aattacagca gtgctgtatt gcagtgggat caggaagtgt atatgcctgt
     3781 aaaaggcgca cattaccgcg cacagcaact gtcaaccctg gcaggttatg cgcatgagtt
     3841 aataactgat aaacgttatg aagagattat cgaaaagctg catgacgata cttccaaact
     3901 tagtcctgtg caatgcgcca atgtgctgga gtcgtacaga atttttaaac agcaaaaaaa
     3961 atatacaaca gaatttgtag ttgcactcaa tcaatgtatc tcaaagagtt ttaatgcctg
     4021 gcaacgggca aagcaagaaa ataattttaa gttgtttgaa ccacatctgc aggaactgat
     4081 aaagtttaaa agacaggagt cggagttgtt gggttatgag caacatccgt atgatgcaca
     4141 tctcaacctt tatgaaacag gattgaagac aagctttctg gaaacaattt tcaatcaggt
     4201 aaggaaaaaa cttgtgccct acatacagca attggtgcag aagcctcagc ccgatgacaa
     4261 ttttttattt aaaagctaca ataaagataa acagtggcat tttgggcttg atgtgttaaa
     4321 gcagatgcac tttgattttg aaagtggcag gcaggacatc tctacgcatc ctttcaccac
     4381 ttccttttct gcaaacgatg ttcgtgtaac tacacgcatc agtgagcatg atttgtttga
     4441 aatgattggc agctgtattc acgaaggcgg acatgcactg tatgaacagg gattgcctgc
     4501 agatgattat ggattgccat caggcgagta tctcagttta agtattcatg agtcacagtc
     4561 gcgcctgtgg gaaaaccatg taggcaaatc gcagtatttc tggaatcata atttcaaaac
     4621 actaagaaat atttttcctg agcagttgca tgatgttagc gaaagaagtt tttacaaggc
     4681 aataaaccgc atcaaaacat cacttatacg taccaatgcc gatgaactta cctatcatct
     4741 gcatgtgtta attcgttttg agattgaaaa agatattttg tcgggagcat tgcaggctgc
     4801 acaacttcct gaagtatgga atgaaaagta taaatcctat ctgaatatag atgtgccttc
     4861 tgacgcaaaa ggggtgttgc aggatgttca ctggagtcat ggtagctttg gttatttccc
     4921 gacatattct ttagggagtt tctatgcatc acagtttttt catcaggcaa ataaagaaat
     4981 tccggcattg atgcatcata ttcaaaatgg tgacaccatg ccgctactga tgtggttaag
     5041 aagcaatata catcgtcatg gaagaacgat actggcagat gagttatgta aaaatgtgac
     5101 gggagaagta cttaacttca attatttttt taattatgca aaaacaaaat acgatgaact
     5161 atatgcttaa caggatgaat tgttgtgcct tggtgctgat agttgccatg gcaggctcgt
     5221 gtaaaacaaa taaaaataca actgcggaaa cgcctaaaca ggagcctgca gcaacaaacc
     5281 cacgtgtaga tttgcccgac agcatgtaca gggttattgt ttcattcatc agcattggtg
     5341 caggaacaga tggtgctgca aaagaaaagt tagagcaggt ggttcagcaa tatcagactg
     5401 aagttggaat gcctgttgca cacgaagaaa ttgcatgggg tagagaagga gaagtagatt
     5461 attgttttag acttcttggt caggataaaa aatcaaaaga ctcttttgta cagcatttaa
     5521 gagatacttt taaaggtaac agcttagttc agattactga aaacactcct gcattgcatc
     5581 aggcaagacc atcattcaac aaataatatt tacgcactta taaaaaaagc cgctatgttt
     5641 tgcaacacag cggctttttg ttttggtttt aagattaatg taccacagaa attttctgcg
     5701 atttaaataa tttttctcct gacatctgta aggtataaat gccttctgcc attcctttca
     5761 ggctaaccga ttctgaaacg gtgttgcccg aaaactgatt ctgataaact atttgtccaa
     5821 cggtgttgaa taatt
//
LOCUS       DAOOOL010000143         5828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1450_length_5828_cov_44.976269, whole genome shotgun sequence.
ACCESSION   DAOOOL010000143 DAOOOL010000000
VERSION     DAOOOL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_5828_cov_44.976269"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKU82_10145"
     CDS             complement(<1..639)
                     /locus_tag="PKU82_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83274.1"
                     /translation="MKSNKWSFKIDKKLYVIFIMLIVITLANAFISTYIIDKSKNITI
                     EIAEVTTPSQSAISDLTQLVNNSWLQATNVAFRKNKTASISELQFINETQYPALKKEL
                     KRLSESWQQKEQLALIGLFEKYDRLIYFELQLIEKARVSTENNVEILETQITPQVQEI
                     TALLSDFNIKKRAETTAKQKEMIQSFIVLMAVVLGLAMVIINFIIVITLFLNK"
     gene            complement(742..812)
                     /locus_tag="PKU82_10150"
     tRNA            complement(742..812)
                     /locus_tag="PKU82_10150"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(778..780),aa:Cys,seq:gca)
     gene            complement(915..1265)
                     /gene="folB"
                     /locus_tag="PKU82_10155"
     CDS             complement(915..1265)
                     /gene="folB"
                     /locus_tag="PKU82_10155"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOZ83275.1"
                     /translation="MHQLILSGIECYAHHGCLSQETVIGGRYRVDITFYADLSIAANS
                     DNLTQTIDYVEVNDMVKIQMAIPSKLIEHVGSRIAQKMKERFTICNKIKVAITKYNPP
                     VQGFIREATFVIEI"
     gene            1350..2885
                     /locus_tag="PKU82_10160"
     CDS             1350..2885
                     /locus_tag="PKU82_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOZ83276.1"
                     /translation="MKIRLVLILTFINFSLLMAQQPITLNVPQPDQDWPNSVTYHIFV
                     RSFCDGNGDGIGDLKGITARWEYLRKLGAEAVLISPIFASPSYHKYDVTDYYKLSPEY
                     GNIESFQYFIKDAHAEGFKVILDIPLNHCSNKHPWFIEAAKNPASTMRNYFVWSNNPP
                     ATEKDKWHLPKDEKGNTIGKTYYYGFFSPEMPDWNYDNAQVVEEAQRILKFWIDLGVD
                     GFRLDAAQHIFKDHNKNIGFWNMISAYVKSINKHTFLVGEVDNSSDMVAPYLKPLDAC
                     FDFDLSREVVQSLKNENASNFPMRMEKIITHYKAVNKDYKDAIFLTNHDQNRVMSEVG
                     NNVDKAKLAANILFTLPGIPYIYYGEEIGMKGEKPDEFIREPMLFAPEKEDEMRPKWM
                     KPKYSTDKTVESAMVQIKEDQSLWNHYSRLISMRKENRALQYGQFENSSLSSKSVLAY
                     YRIYNQMRVLIINNVSATAVTLTKEEKDNTLINPIYQTSKQTTVKGNKITLAPYSTII
                     LTN"
     gene            complement(2992..4530)
                     /gene="gltX"
                     /locus_tag="PKU82_10165"
     CDS             complement(2992..4530)
                     /gene="gltX"
                     /locus_tag="PKU82_10165"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOZ83277.1"
                     /translation="MQRRIRTRFAPSPTGPLHMGGVRTALYAWLFAKKNGGDFLLRIE
                     DTDQNRFVPGAEQYIIDALHWCGITIDEGVSVGGTHAPYRQSERKEVYRSYAMQLVAS
                     GNAYYAFDTAEQLEQQRKNAEEKKETFSYGSATRMSLNNSLALTVEEVKELISKNVPY
                     VIRLKVQENETITFTDLIRGEVSFDSNLVDDKVLLKSDGMPTYHLAHIVDDYLMEITH
                     AIRGEEWLPSAPAHILIYRYLGLTAQMPHYAHLPLLLKPDGKGKLSKRDGDRLGFPVF
                     PLEWKDPTTGEVSSGYRENGYYPEAFVNMLAMLGWNPGTEQEIFNMNELIAAFSIDHV
                     HKAGARFDPEKTKWYNTQWMLRQSDEDIAKRIKPMVASLTGITTDDVRLSDSFLTACV
                     ALLKPRVQFEKEMPEKGLYLFIEPTEYDQTVISKKWKPEFKDFFKMLSDEFTASNEWN
                     SQIAEQTFKAAAEKNNLKPGEVLQLFRVFVSGQAQGVDLFPMIQLLGKNQVLKRLESA
                     LKLV"
     gene            complement(4576..>5828)
                     /locus_tag="PKU82_10170"
     CDS             complement(4576..>5828)
                     /locus_tag="PKU82_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOZ83278.1"
                     /translation="SKGGRVLHMLRKYVGDDAFFASLKKYLNDNKFSPVEAHNLRLAF
                     EAVTGQDLNWFFNQWFFAKGHPIIDISYQWNDTLKKQTVIIEQKQNLQETPLYKLPLA
                     IDIYENGKVRREQVTLTQQKEYLFFDCNSKPQLINVDAEKQLLCAKKDNHTSDEWRYQ
                     YQHAPLFLDRYEAIDALAKGYEGNSAEAKIIISALDDKNPSIRALALRNIGAAAKADS
                     TVKNKLQQMVTKDAKAHVRESALSTLYKYFDDSSLDNIAINDSSYNVMTTALEHYITK
                     NPKQLSSLKKYESDNNNNLLYTLSGLYAQYGSDENYDYMINALNVASGFGKMGIIQNL
                     GTFLEHSSPAVTEKGVDALTDAAKNSSSKFMNMQTSNTLSKLKKFVTNKIKELETKPE
                     YFDQLSRYKKIEEKLTSSITSLKK"
BASE COUNT         1775 a         1023 c         1120 g         1910 t
ORIGIN      
        1 tttgttcaga aacaaagtaa ttacgataat gaaattaatt atcaccatgg ctaaacctaa
       61 aacaactgcc atcaaaacaa tgaatgactg aatcatttct ttttgtttgg ctgtagtctc
      121 agcacgcttt tttatgttga agtcgcttag tagtgcagta atttcctgta cctgtggggt
      181 aatttgcgtt tctaaaattt ctacattatt ctctgtacta acacgagctt tctcaatcaa
      241 ctgcaattca aaataaatta aacggtcgta tttttcgaat agaccgatta gggcaagctg
      301 ttctttttgt tgccaagatt cagaaagacg tttaagttct ttttttaatg caggatattg
      361 tgtttcatta ataaactgaa gttcgcttat ggaggcagtt ttgtttttgc gaaaagctac
      421 attggttgcc tggagccatg aattgtttac caattgtgtt aagtcggaaa ttgccgattg
      481 tgagggtgta gttacttctg caatttcaat ggtgatgttt ttgcttttgt caataatata
      541 ggtgcttata aaggcattgg ctagggtgat aacaataagc atgataaaaa tgacgtaaag
      601 ctttttatca attttaaaag accatttatt ggatttcatt gctccggtta taaaaaatat
      661 taaaaattat acgaggatac ggggtaaatg gttacctgaa taaaacaaaa aatcccgcta
      721 agagaggcgg gattttgagt gaggtcgcga gcagattcga actgctgtac aaggttttgc
      781 agacctctgc ctagccactc ggccacgcga ccctatttac tggcggcaaa tgtaaaaata
      841 ttttgcttgc ggcaatagga atgaaaatgc tgaaataaat ggttttaaac gttaaataaa
      901 caaaaccggt tacattaaat ttcaattaca aatgttgctt cacgtataaa accctgtact
      961 ggaggattgt atttggttat agccactttt attttattac agattgtaaa acgttctttc
     1021 attttttgtg caatgcggct gccaacatgt tctattagtt ttgaaggaat tgccatttgt
     1081 attttaacca tgtcattgac ttcgacataa tcaatagtct gtgtcagatt atcggagttt
     1141 gcggcaatac tcaggtcggc ataaaaagtt atatccacac gatagcggcc accaataacg
     1201 gtttcttgtg ataaacagcc atggtgagca taacattcaa ttccggataa aataagttgg
     1261 tgcataaagc aaaattttgg atacaaaagt aattcattgg caaatgattc tttttattta
     1321 atatcgcctt ttatttatga aatatagaaa tgaaaattcg tcttgtttta atactcactt
     1381 ttataaactt tagtctcctg atggctcaac aacctataac actaaatgtg cctcagcctg
     1441 atcaagactg gcctaacagt gttacctatc atatttttgt ccgctcgttt tgtgatggca
     1501 acggtgacgg cattggcgac cttaagggca ttactgccag atgggagtac ctcagaaagt
     1561 taggtgcaga agcagtatta ataagtccga tttttgcttc accatcgtat cataagtacg
     1621 atgtaacaga ttactataaa ttatcaccgg aatatggaaa catagagtcg tttcaatatt
     1681 ttataaaaga tgctcatgcc gagggattta aagtaatttt agatatacca ttaaatcatt
     1741 gcagcaacaa acacccctgg tttatagagg cagcaaaaaa tccggcaagt acaatgcgaa
     1801 actattttgt gtggagcaac aatccgcctg caacagaaaa agacaaatgg catctgccaa
     1861 aagatgaaaa aggaaataca ataggaaaaa catactatta tggttttttc agtcctgaaa
     1921 tgcctgactg gaactatgac aatgcgcagg tagtggagga agctcaacgc attttaaaat
     1981 tttggattga tttgggagta gatgggttca gattagatgc tgcacagcat atttttaaag
     2041 atcacaataa aaatattggc ttttggaata tgatttcggc ctatgtaaaa agtatcaaca
     2101 agcatacatt tttagtaggt gaggtggata attcttcaga tatggtagca ccctatctta
     2161 aaccactcga tgcctgcttt gattttgatt tatcgcgtga agttgttcaa tcactgaaga
     2221 atgagaatgc aagtaacttc cctatgagaa tggaaaaaat tatcacacac tataaagcag
     2281 tgaataagga ttataaggat gctatttttc ttactaatca tgatcagaac agggtgatga
     2341 gtgaggttgg aaataatgtt gacaaagcaa agcttgctgc caatatttta tttacgcttc
     2401 caggtattcc atacatttat tatggagaag aaatagggat gaaaggtgaa aagcctgatg
     2461 aatttattcg tgagcccatg ttatttgcac ctgaaaaaga agatgaaatg cgccccaaat
     2521 ggatgaagcc taaatacagt acggataaaa ccgttgagtc ggcaatggtt cagataaaag
     2581 aagatcagtc gctatggaat cattattcaa gacttatttc tatgcgcaaa gaaaacagag
     2641 cattgcaata cggtcaattt gaaaactcat ctttgagtag taaaagtgta ttggcttatt
     2701 atcgtatcta taatcagatg cgtgttttga taattaacaa cgtaagtgcc acagccgtta
     2761 cactaacaaa agaggaaaaa gataacacgc ttataaatcc gatttatcag acatcaaaac
     2821 aaactacagt taaaggaaac aaaattacgc ttgctccata cagtacaatt attctcacca
     2881 attaaaagat atttaaaact tatattagtc ctgaaaggat gtaatttaca agagtagcgt
     2941 gaagtagtgc cctttcaggg tcaagataat atattacaat ttccgctatt attagactaa
     3001 cttcaaagca gattctaatc ttttaagcac ctggtttttt ccaagcagct gtatcattgg
     3061 aaaaaggtca acgccttgtg cctgtccact aacaaaaaca cgaaagagtt gaagtacttc
     3121 accgggttta agattatttt tttcggcagc ggctttaaaa gtctgctctg caatctgact
     3181 gttccattca tttgatgcag taaattcatc agaaagcatt ttgaaaaaat ctttaaattc
     3241 aggtttccat tttttagaga taactgtctg atcatattcc gttggctcta taaaaagata
     3301 caatcctttt tcaggcattt ctttctcaaa ctgaacacga ggttttagca atgccacaca
     3361 tgctgttaga aaactatcag agaggcgcac atcatccgta gtgattcctg tgagtgatgc
     3421 aaccataggt ttaatacgtt ttgcaatgtc ttcatcggat tgtcgcagca tccattgagt
     3481 attgtaccat tttgtttttt cagggtcgaa gcgtgctcct gctttgtgaa catgatctat
     3541 agaaaatgca gcaatgagtt cgttcatatt aaaaatttcc tgctctgttc cgggattcca
     3601 tcccaacata gcaagcatgt taacaaatgc ttcggggtag taaccatttt cgcggtagcc
     3661 gctagacacc tcacctgttg tagggtcttt ccattcaaga ggaaagacag gaaatcctaa
     3721 cctgtcgcca tcgcgcttgc tgagttttcc ttttccatca ggtttcagca ataaaggaag
     3781 gtgtgcatag tgaggcattt gcgcagttaa tccgagataa cgataaatta aaatatgtgc
     3841 aggtgcagaa ggcaaccact cttcgcctct gatagcatgt gttatttcca tcaggtagtc
     3901 atcaacaata tgtgctaaat gataggtagg catgccatca cttttaagca acactttatc
     3961 atcaactaaa ttagagtcaa aactgacttc accacgaatg agatctgtaa aagttattgt
     4021 ttcattttcc tgaactttca gtcggataac ataaggaaca ttctttgaaa ttagttcttt
     4081 aacttcttca acagttaatg ccaatgaatt attaagactc attcttgtag cactgccata
     4141 agaaaaagtt tctttttttt cttctgcatt tttgcgctgt tgttctaatt gttctgcagt
     4201 atcgaatgca taataggcat ttccggatgc gacaagttgc atggcatagc ttctgtaaac
     4261 ttctttccgt tcactctgtc tgtatggtgc atgtgtgccg ccaacagaaa cgccttcgtc
     4321 aatggtgatg ccgcaccagt gcagtgcatc aatgatatat tgttcggcac caggaacaaa
     4381 gcggttctga tcggtatctt ctatacgtaa taaaaaatcg cctccatttt tttttgcaaa
     4441 cagccaggcg tataatgctg tgcgcacacc gcccatgtgt agtggcccgg tggggctggg
     4501 ggcaaatcgt gttcttattc ttcgctgcat aaaataaatt ctgctgcaaa gataatcgag
     4561 aaaaagttgt gtggtttatt tctttagtga agttatactt gaagtgagtt tttcttcaat
     4621 ttttttgtag cggctcaact gatcaaaata ctcaggcttt gtttccagtt cttttatttt
     4681 gttagtgaca aattttttga gcttactaag tgtattggaa gtttgcatat tcataaactt
     4741 tgagctgctg ttttttgcag catcagtcaa agcgtcaaca cctttttctg tcacagcagg
     4801 tgaactgtgc tcgaggaaag ttccgaggtt ttgaataatt cccatctttc caaatccgga
     4861 tgctacattt aatgcattta tcatgtaatc gtaattttca tcactgccat attgtgcata
     4921 aagtcctgag agtgtataaa gaaggttgtt gttattatcg gactcatatt tttttagtga
     4981 ggacaattgc tttggatttt ttgtgatgta atgttccaat gcagtagtca ttacattgta
     5041 tgaagagtcg tttatagcaa tattatctaa agaagaatca tcaaaatatt tatataaggt
     5101 tgacaaggca gattctctta catgtgcttt agcatcttta gttaccattt gttgtaattt
     5161 gttttttact gtactgtcag cttttgctgc tgcaccaata tttcttaaag caagtgctct
     5221 gatagaagga tttttgtcgt ctaatgcgct gataataatt tttgcttctg cactattgcc
     5281 ttcataacct ttggctaatg catcaatagc ttcgtaacgg tcgagaaaca atggcgcatg
     5341 ctgatactga tacctccatt catcgctggt gtggttgtcc tttttagcgc ataacaattg
     5401 tttttctgca tctacattta tcaattgtgg cttgctgttg caatcaaaaa ataaatattc
     5461 tttttgttgt gtaagtgtaa cctgttcgcg tctaactttt ccgttttcat aaatatctat
     5521 tgcaagagga agtttataga gtggtgtttc ctgaagattt tgtttttgtt cgatgatgac
     5581 ggtttgtttt ttcagagtat cgttccactg ataactgata tcaattatgg gatgaccttt
     5641 agcaaaaaac cactgattga aaaaccagtt gaggtcttgt ccggtaacag cttcaaaagc
     5701 caaacgcaga ttatgtgctt caacagggct gaatttattg tcgtttaaat atttttttaa
     5761 tgatgcaaaa aaggcatcat cgccaacata tttacgaagc atgtgtaaca cccgaccccc
     5821 ttttgaat
//
LOCUS       DAOOOL010000144         5798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1465_length_5798_cov_80.030994, whole genome shotgun sequence.
ACCESSION   DAOOOL010000144 DAOOOL010000000
VERSION     DAOOOL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_5798_cov_80.030994"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..599
                     /locus_tag="PKU82_10175"
     CDS             78..599
                     /locus_tag="PKU82_10175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOZ83279.1"
                     /translation="MSNEITIRQETPDDYLDVFKLNQVAFGQEYEAKLVDALRSNTDV
                     FIPELSIVATINNKIVGHILFTKIAIKDDSGNLNESLALAPMAVLPEFQKTGIGGQLI
                     SEGLAVAKGLKFRSVIVLGHEHYYPKFGFQPAIKWNIKAPFDVPSNAFMAIELEQGGL
                     RNISGTVIYPKEF"
     gene            complement(621..2348)
                     /locus_tag="PKU82_10180"
     CDS             complement(621..2348)
                     /locus_tag="PKU82_10180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOZ83280.1"
                     /translation="MSLTTENIGKANRHFLKSDFSVTSWDDLKPYYENLISRTINSKE
                     DLIEWLQDRSELESALQENLAWRYIRMSCDNTNEKLQQDYNYYVREIDPNISPEDNKL
                     NEKFIASPFSELLSETDYRNYLRNVKQRISLFRKENIPLLAELAEKQQIFGQISGAQT
                     ITYNNQELTLQQASVWLKNTDRSIRETVYKKITARRLEDKKQLDDLYTNLIQLRHQVA
                     LNAGFKNFRDYSFAALERFDYSVEDCYRFHESIESEIKPVINQIEEYRKTKLGVAHLM
                     PWDLAVDPTGKPPIKPFNNTQEMVEKTISCLKEIDSEFANCITLLDEMNRFDLDSRKG
                     KSPGGYNYPLYETGVPFIFMNSTGQLRDLVTMVHESGHAVHAVLAHNLPFHEMRSCPS
                     EVAELASMSMELLTMEHWHHFISDEKELKRAKLEHLTSLIDILPWIAIVDAFQQWVYM
                     HPEHSVAEREQEFVNLYNRFSGSVVDWQGNEKVKENLWQKQIHIFDSPFYYIEYGMAQ
                     LGAIALWKNYKTDATTTISNYKKALSLGYTKSIGEIYAAAGVRFDFSATYIKELSQFV
                     QSEIDALQK"
     gene            complement(2352..2870)
                     /locus_tag="PKU82_10185"
     CDS             complement(2352..2870)
                     /locus_tag="PKU82_10185"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOZ83281.1"
                     /translation="MSLKKIMFISGMPGLYQLVAQSRNGFIAESLADKKRLHVSNNQQ
                     VSMLSDISIFTTADDVKLAEVFRKMTALDEKLTKIDLNADGSVILSSFEKILPEFDKD
                     RVYTSDIKKVFKWFNLLKDKIDLAAKEETADDAATENTAPAEAEKTEIVEETEAKPKA
                     KRKTKKAENDKA"
     gene            3045..5006
                     /locus_tag="PKU82_10190"
     CDS             3045..5006
                     /locus_tag="PKU82_10190"
                     /inference="COORDINATES: protein motif:HMM:NF026223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-repeat neuraminidase N-terminal
                     domain-containing protein"
                     /protein_id="HOZ83282.1"
                     /translation="MFKRKKLLSQTPSMRTNLRMAMYIGAASLSGLVVLLLIVFNISK
                     REISKAAYSGMQVINAEVFQNTDAVYRGSINNPIIGILIDVKGYKTAVKLNSITFSLK
                     GTTQPYFKQVENIKVWSSGQTKAFSGLTQLYQSVAPSDGNVRIDINSMLSPGLNYLWI
                     STDIKASAMPTAVIDAELTALTVNGSPLLPLLASAPGSKKIKNNTALFSVRSGNFNDL
                     SLWNTQRDGKGSAPATISPNTCFFIQGGHEFTLSESDTSSYAVVESKGKLNGDKHYGL
                     KEVCVEQGAIFQLNENENGAGNIVHLILKDGSNYIQNSSGSFASDNAQLSNSSQVVFM
                     KYSNHSFGKDIAWGNVMFDAQAGVDANIGQAFRNVYGNLELRNTGKGSLYTEGVSIMS
                     VKGNFIVSGGRFEGVREVNSKLVLNVGGNFMVQAGSVYDVANNSTPGARTTLNLYGDV
                     MLLGGKVMLNNADDDGSFINMMGFENPQVRWMQTDKAQVELCNVAIKPEKEVFLKGNK
                     LGEIPAGRLLSVERNAKLWCATYSVTGQGRFSLADMATLGIGHPKGINSGLNEGNILT
                     AERQFNSGASYVYYLSTTPQEMGQFATFPKEKTIRSLTIKKDSPVQQVNLSTDLFVSD
                     QVKVSMGEFNRSGFKLQLSDVSETSGKFH"
     gene            5146..5727
                     /locus_tag="PKU82_10195"
     CDS             5146..5727
                     /locus_tag="PKU82_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83283.1"
                     /translation="MKKLICAALIAAGMTTMATTTKAQGGDPPFVKGSNTIGLSIGFG
                     DYYDYGSVYYGSSWTVLPSINLHYDHGTIAEVGPGTIGIGGIIGIKNRYYKYYNGDKY
                     TDNSVIIGVRGTYHLTLLADKNNKFDPYGGVLLGARIRTRNDHYYDDKRNNVYPTTGL
                     FVGAKYNFVENFGAFAELGYDISFFKVGINLNF"
BASE COUNT         1779 a         1012 c         1130 g         1877 t
ORIGIN      
        1 ttggaatctg ctttttacat atttttgaag agatatgtat atgtgaaggt tagtagctat
       61 tatatacaaa tgaaaccatg agtaatgaga taaccattcg ccaagaaaca cctgatgatt
      121 atttagatgt ttttaaatta aatcaagttg ccttcggtca ggagtatgag gctaaattgg
      181 tggatgcatt gagaagcaat accgatgttt ttattcctga actttctatt gtagctacta
      241 tcaacaataa aattgtcgga catattttat ttactaagat tgcgataaaa gatgattctg
      301 gaaacctcaa tgaaagttta gcattggcac ctatggcagt gttgcctgaa tttcagaaaa
      361 caggcatcgg tgggcaactt atcagtgaag gattagctgt agctaaaggt ttaaagttta
      421 ggtctgtaat agtactcggt catgaacact attatcctaa atttggtttt caacctgcaa
      481 ttaaatggaa tattaaagca ccatttgatg ttccttcaaa tgcttttatg gcaatagaac
      541 tggagcaagg cggactaaga aatatatcag gcacggtaat ttatccaaag gaattttaag
      601 aaaaaatcag ctctaaaatc ttacttctga agagcatcaa tttctgattg tacgaactgc
      661 gaaagttcct taatgtatgt tgcagaaaaa tcaaagcgta caccggcagc agcataaatt
      721 tcaccaattg attttgtata accaagtgac aatgcttttt tgtaattgct gatagtagta
      781 gtagcatcag ttttataatt cttccataat gcaatagcgc ctaattgcgc cattccatat
      841 tcaatgtaat agaaaggtga atcaaaaatg tgaatttgtt tctgccacaa attttctttt
      901 actttttcat tgccctgcca gtcaacaaca ctgccactaa aacgattgta taggtttaca
      961 aactcctgtt cgcgttccgc cacactatgt tcaggatgca tatataccca ctgttgaaat
     1021 gcatctacaa tggctatcca tggcagaatg tcaattaggc tggtaagatg ttcaagtttg
     1081 gcacgtttca attctttttc atcagaaata aaatgatgcc aatgctccat tgtcaacaat
     1141 tccatggaca tagaagcaag ttctgcaact tcagaagggc agcttcgcat ttcatgaaaa
     1201 ggaagattgt gtgccagcac agcatgtact gcatgtccgc tttcatgtac cattgtcacc
     1261 agatcgcgca actgtcctgt gctgttcatg aaaataaatg gcacacctgt ttcgtacaaa
     1321 ggatagttgt atccccctgg tgatttgcct tttcgcgaat ccaaatcaaa acgattcatc
     1381 tcatcaagca gtgtgatgca atttgcaaac tccgaatcaa tttcttttaa acatgaaata
     1441 gttttctcaa ccatctcctg cgtgttattg aaaggcttta ttggaggttt tcctgtaggg
     1501 tcaacagcca aatcccaagg cattaagtgc gcaacaccaa gtttagtttt tctgtattct
     1561 tcaatctgat tgataacagg ttttatttcc gattctatac tttcgtgaaa ccggtaacaa
     1621 tcttcaacgc tgtagtcaaa tcgctctaat gctgcaaagg aataatctct gaaatttttg
     1681 aagcctgcat tgagtgccac ctgatgccga agctgaatta aattggtgta taagtcatct
     1741 aactgctttt tgtcttctaa tcgtctggca gtaatttttt tgtaaacagt ctcacgaata
     1801 cttctgtcag tattttttaa ccataccgat gcttgttgta aagtaagttc ctgattgtta
     1861 taagtgatgg tttgcgctcc cgaaatctga ccgaatattt gttgcttttc tgctagctct
     1921 gcaagcagtg gaatattttc ttttctgaat aaagaaatac gttgctttac attgcgtaga
     1981 tagtttctgt agtctgtttc agaaagcaac tctgaaaaag gactggcaat aaatttttca
     2041 ttcaacttat tgtcttccgg agaaatgttc gggtctattt ctctaacata ataattataa
     2101 tcctgttgta gtttttcatt cgtattgtcg cagctcattc tgatataacg ccacgccaaa
     2161 ttctcctgca atgccgattc taattcgctt ctgtcctgca gccattcaat taaatcttcc
     2221 tttgagttta tggtgcgcga aatcaggttt tcatagtacg gttttaaatc atcccacgat
     2281 gttacactaa aatcactttt aagaaaatgg cggtttgcct ttccaatatt ttctgttgtc
     2341 agagacataa atcaggcttt atcgttttct gctttttttg ttttgcgctt tgcttttggc
     2401 tttgcttcag tttcttcaac aatttctgtt ttctctgctt cagcaggagc agtattttct
     2461 gtagctgcat cgtctgcggt ttcttctttt gctgctaaat caattttgtc tttaagtaag
     2521 ttaaaccact taaacacctt ttttatgtct gatgtataaa ccctgtcttt atcaaactca
     2581 ggtagaattt tttcaaatga cgataaaatt accgacccat ctgcattcaa atctatctta
     2641 gtcaatttct catctaatgc agtcatcttt ctgaaaactt ctgccaattt aacatcatca
     2701 gcagtagtaa aaatactgat gtcggaaagc atacttactt gttggttgtt gctgacatgc
     2761 aatcgttttt tatcagcaag cgattctgca ataaaaccat tacggctttg tgcaactaat
     2821 tggtacaaac cgggcatgcc ggagataaac atgatttttt tcaaactcat tttctataat
     2881 attctgatta ataaggcgac aaagttaaaa ttttataact gcctgagagc ctgatttttt
     2941 ccacattcat ataacctgca ttcaggcaat tatgagttta tgctattgaa acctatttaa
     3001 ttatatacag tataaggtca ggttaagatg ttaatattct aatcatgttt aagcgaaaaa
     3061 aattacttag tcaaactccg tctatgcgca ccaacctgcg tatggccatg tatattggag
     3121 cagcttccct gtcgggcctt gtggtgctgt tactcatagt ttttaatatt tcgaaacgcg
     3181 aaatatcgaa agcggcttat agtggcatgc aggttattaa tgcagaagtt tttcagaata
     3241 ccgatgctgt ttaccgtgga agtattaaca atcctataat cggaattttg attgacgtaa
     3301 aaggatataa aactgcggtt aaacttaact caataacttt ttcattaaag ggaacaacac
     3361 agccatattt caagcaggtc gaaaatataa aagtctggtc atccggacag acaaaagcat
     3421 tttcaggact cacacaactt tatcagtcgg tagcaccttc tgatggcaat gtgcgaatag
     3481 atattaacag tatgctttca ccgggtttaa attatttgtg gataagtaca gatattaagg
     3541 cttcggcaat gcccactgcg gtaattgatg cagagcttac tgcgctaact gtaaatggtt
     3601 ctcctttgtt accattgctt gcatcagcac ccggaagtaa aaaaataaaa aataatacag
     3661 cacttttttc tgtgcgttca ggtaatttca acgacctttc cttgtggaat acacaacgcg
     3721 atggtaaagg ttcagcgcct gcaactattt ctccaaacac atgctttttt attcagggcg
     3781 gacatgagtt tacattgagt gaaagtgaca catcttccta tgctgtagta gagagcaaag
     3841 gaaagttgaa tggcgataag cattatggat taaaggaagt ttgtgtagag caaggtgcca
     3901 ttttccaact caatgaaaat gaaaatgggg caggcaatat tgtgcattta attttaaaag
     3961 atggctcaaa ttatattcaa aacagctcag gcagttttgc ttctgataat gcacaattat
     4021 caaattcatc acaagtagta tttatgaagt atagtaacca ttcttttgga aaagatatag
     4081 cttggggtaa tgtaatgttt gatgcacagg caggtgttga tgccaatatt gggcaggcat
     4141 tcagaaatgt atatggaaac ctcgaactgc gtaataccgg aaagggttct ttatatacag
     4201 aaggcgtaag cattatgtcc gttaaaggaa attttattgt ttccggagga aggtttgaag
     4261 gtgtaagaga ggttaacagt aaattagttc tcaatgtagg tggcaacttt atggttcagg
     4321 caggtagtgt ttatgatgtt gcaaacaata gcacgccagg tgcacgcaca actttaaatc
     4381 tttatggcga tgttatgtta cttggcggaa aagttatgtt gaacaatgct gatgacgatg
     4441 gttcatttat taatatgatg ggttttgaaa atccgcaggt tcgttggatg cagacagata
     4501 aagcgcaggt tgaattgtgt aatgtagcca taaaacccga gaaggaagtt tttctgaaag
     4561 gaaataagtt gggtgaaatc cctgcaggta gattattgtc tgttgagcgc aatgcaaaat
     4621 tgtggtgcgc tacctacagt gtaacaggac agggtaggtt tagccttgcg gacatggcaa
     4681 cattgggtat aggccatcca aaaggaatta attccggttt aaacgagggg aatattttaa
     4741 cggcagaaag acaatttaat tcaggcgcaa gttatgttta ttatctgtca acaacacctc
     4801 aggagatggg gcagtttgcc acattcccaa aagaaaaaac catacgcagc ttaacgatta
     4861 aaaaggacag tcctgtgcaa caagtaaatt tgtcaaccga tttatttgtg agcgatcagg
     4921 taaaggtaag catgggcgag tttaaccgtt caggatttaa gctgcaatta tccgatgtca
     4981 gcgaaacatc aggtaagttc cattaaaaaa attctgttaa tagtttttta agtcggcaaa
     5041 aatgccggct tttttatttt ttatggttaa taaattcatt gaaaggtaat ttaagtaatc
     5101 aaacttctat ttaagtttgt gccataaaac aaagaaaata gaaaaatgaa aaaactgatt
     5161 tgcgctgcct tgattgcagc aggaatgaca acaatggcaa caacaacaaa agctcagggt
     5221 ggtgacccac cttttgttaa aggctcaaac actattggct tgtcaatagg ctttggcgac
     5281 tattacgatt acggaagtgt gtattatgga agcagttgga cagtgcttcc atcaataaat
     5341 ttacattatg atcatggaac tattgcagaa gtaggtcccg gtactatagg tattggtgga
     5401 ataatcggaa taaaaaaccg ctactataaa tattataatg gtgataaata caccgacaac
     5461 agtgttataa ttggtgttcg cggtacctat caccttacat tattggcaga taagaacaat
     5521 aaatttgatc cttatggtgg tgtattgttg ggagcgcgta tcagaaccag aaacgaccat
     5581 tattacgatg acaaacgaaa taatgtatat ccaacaacag ggttgtttgt tggtgctaaa
     5641 tataatttcg tagaaaactt tggcgccttt gccgagttgg gttatgatat ttcattcttt
     5701 aaagttggta ttaatctcaa cttttaatta aaattaaaat ttgaaaaacc ggtagttgat
     5761 ttttagctgc cggttttttt attgacgtaa gtttagaa
//
LOCUS       DAOOOL010000145         5772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1472_length_5772_cov_49.979010, whole genome shotgun sequence.
ACCESSION   DAOOOL010000145 DAOOOL010000000
VERSION     DAOOOL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_5772_cov_49.979010"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..236
                     /locus_tag="PKU82_10200"
     CDS             42..236
                     /locus_tag="PKU82_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOZ83284.1"
                     /translation="MSSVTCLLLWSLGGSEILLIVLVLLLFFGGKKLPELARGLGKGI
                     KEFKDASSGVEKDEEKKENK"
     gene            340..1302
                     /locus_tag="PKU82_10205"
     CDS             340..1302
                     /locus_tag="PKU82_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOZ83285.1"
                     /translation="MKNLHLIIVAFWCLVSSCDNTSKNIPSIGFLDIAEDATLAKART
                     GFLDALSKNGFSEKDNTLKIYFSNAQGEIAVLNQACDYLISKNVKLIAANSTLSTIAA
                     VQHSKDIPVCMMVAPRPDLAGLADAQGNYPKNLFGVFETLAYIDSSVALIKRFFPDAV
                     NVGTIYNSSEPQSRDALEELQKAATTSGMKIIALPVTNSSETQLVTQSLIAKKPDVFF
                     ALPDNVIFASFETVVKACNEAQLPVFTSEAGLVSRGAIASYGADFYQWGFQAGVEAAN
                     YLKSNTATIPKPELVQVRQYVFNNKAATRFNLVAPNGFIVIDSH"
     gene            1302..2153
                     /locus_tag="PKU82_10210"
     CDS             1302..2153
                     /locus_tag="PKU82_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83286.1"
                     /translation="MEVIIPAIIQGLCYAAMAFGIYLTLRIFNIPDITTDGSYTLGGV
                     VAAILISNHWHPAVALPIVLAAGALAGVATGLIHTQLKLHPLLAGILVMTSLYSINLF
                     IMGRPNIPLINEASIFNGSTSFNMLLAAMFSIILLLLLFWLMKTDFGIAMRATGSNEL
                     MVKAMGININLMKIAGLAIANALTALSGYLMVQYQGFADINMGIGIVIAGLAAVILGE
                     SFAVFFKSSSIFSALITVLLGSVLFRLLLAFALLAGVPPQMLKLLTAVLVLLAAGVFK
                     FIFKPAQ"
     gene            2150..2896
                     /locus_tag="PKU82_10215"
     CDS             2150..2896
                     /locus_tag="PKU82_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ83287.1"
                     /translation="MIEVSQISKKFFDGEKPVIALQPTSLQIKVKEFVIIVGANGSGK
                     STLLNIIAGTILCDAGKIAVNGKDITALKEHERSRFIARVFQNPQSGTASDLTIIENF
                     RLAALRSHSKKLSIGIDKKFVDFIRERVSWLGLGLEDQLNRKMGALSGGQRQALTVVM
                     STMDDFKLLLMDEPTAALDPKSAELIMQLTTRILSQMPVSVLMVTHQTKQVIDYGNRV
                     IQMSSGQIIRDLDKASKEKLTLNEIIHWFD"
     gene            3031..4962
                     /gene="dxs"
                     /locus_tag="PKU82_10220"
     CDS             3031..4962
                     /gene="dxs"
                     /locus_tag="PKU82_10220"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOZ83288.1"
                     /translation="MEIKAGELLSSINTPDDLRKIKPEQLEQVCKELRQFIIDIVSVN
                     GGHFGASLGVVELTVALHYVFNTPNDQLIWDVGHQAYGHKILTGRRDVFHTNRIYKGI
                     SGFPKRSESVYDTFGVGHSSTSISAALGMSVASRLKDDKNRQHIAVIGDGAMTAGMAF
                     EALNHGGVENSNLLVVLNDNCMSIDPNVGALKEYLTDITTSPTYNKVKDEIWKALGVI
                     SKFGPNAQAIAQKIENAMKSAVLRQSNLFESLRFRYFGPIDGHDVNHLTKVLEDLKNI
                     PGPKILHCLTVKGKGFKLAEQDQTKWHAPGLFDKITGEIFKPQNNTPQPPKYQDVFGH
                     TMVELAEKNSKIVGVTPAMPSGCSLNIMMKAMPDRAFDVGIAEQHAVTFSAGMATQGL
                     IPFCNIYSSFMQRAYDQVVHDVALQKLNVVFCLDRGGLAGADGPTHHGAFDIAYMRCI
                     PNMIVSAPMNEEELRNLMYTAQHDNLGPFCIRYPRGNGVLTEWRTPFKAIPVGTGRKL
                     RGGEQVAILTFGHPGNFAVAACDELAKENIYPAHYDLRFVKPLDQMLLHEVFANFKKI
                     ITVEDGCLQGGMGSAVAEFMIDNGYAAEIVRLGIPDQFIEHGEQSELYAECHFDTQSI
                     MKTVRQLIAASHPGIKSLK"
     gene            complement(5117..>5772)
                     /locus_tag="PKU82_10225"
     CDS             complement(5117..>5772)
                     /locus_tag="PKU82_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83289.1"
                     /translation="ISRTEVSNGSSAVIATVPASTLYYFDSTMICPDDYSYRIKAYDL
                     CGTTYTSYSDTAAAKPDYNLQEQQAIIVRSTVVNNNFVLTEWSEPRLHPERVMQYNIY
                     RSTDTTGNFYSLLATVSANVTSYEDFAVNVNEQNYYYKIEVISDCNMAGQLSSNSSSI
                     LLQSKWEYANSRLWWTKYDKWDTGVERYEIEKYNWGTGQWEKVKTVPGNATETEVDE"
BASE COUNT         1715 a         1019 c         1190 g         1848 t
ORIGIN      
        1 acattataat ttcttgtata tttgtaccat atatttaaag aatgagttct gttacttgtt
       61 tactgctttg gagtttaggt gggtctgaaa tactgctgat tgttttagtg ttgttgttgt
      121 ttttcggagg aaaaaaatta cctgaattgg caagaggact tggtaaaggg ataaaagaat
      181 ttaaagatgc atcatccggt gttgaaaagg atgaagaaaa aaaagaaaat aaataaacaa
      241 cgatagtttt catggcttcg ggacgtttgc gcaagcagcg tcccgttttt catttctgat
      301 agtttagatt ttcttagttt tataccttca ttgttcatta tgaaaaacct gcatttaatc
      361 attgttgcat tttggtgctt agtttcatcg tgcgataaca cttcaaaaaa tattccgtca
      421 attggatttt tagatattgc agaagatgca actttagcaa aggcacgaac aggattttta
      481 gacgcattgt caaagaatgg cttttcagaa aaagataata ctctaaaaat ttatttttca
      541 aacgcacagg gtgaaattgc tgttttgaat caggcatgtg attatttgat ttcaaaaaat
      601 gtaaaactca ttgcagccaa ttcaacgctt tcaacaatag cggcagtgca gcattcaaaa
      661 gatattcctg tttgtatgat ggtagcacca cgccctgatc ttgcaggact cgcagatgcc
      721 caaggaaatt atcctaaaaa tctctttggt gtttttgaaa cattggcata tattgacagc
      781 tcagtagcat tgataaaacg tttctttcct gatgcagtga atgttggcac catttacaat
      841 tccagcgaac cacaatcgcg tgatgcgctt gaagaattac aaaaagcagc aacaacatcg
      901 gggatgaaaa ttattgcctt gccggttaca aattcttcag aaacacaact ggtaacacaa
      961 tctctcattg caaaaaagcc tgatgtgttt tttgcgctcc ccgacaatgt catttttgca
     1021 tcattcgaaa cggtggttaa ggcatgtaac gaagcacaac ttccggtttt taccagcgaa
     1081 gctggcttgg ttagtcgtgg agctattgca tcctatggtg ccgattttta tcaatggggc
     1141 tttcaggcag gtgtggaagc agcaaattat cttaaatcaa acactgcgac aatccccaaa
     1201 ccggaacttg ttcaagtcag gcaatatgta ttcaacaata aagcagcaac tcgtttcaat
     1261 cttgttgcac ccaatggttt tatagtaatt gattcacatt aatggaagta attattcctg
     1321 caattattca gggcttgtgc tatgctgcta tggcatttgg catttatctg acattgcgga
     1381 tttttaatat accggacatt accaccgatg gcagttatac attaggcggt gttgttgctg
     1441 caattttaat ttcaaatcat tggcatcctg ctgtagcatt gcccatagta cttgctgcag
     1501 gtgccttggc aggagttgca acaggtctca ttcacacaca actgaagttg catccgttgc
     1561 ttgcaggtat tttagtaatg acatcactct attccatcaa cctgtttatt atgggaagac
     1621 ctaacattcc attaatcaat gaagcaagta tttttaatgg gagcacaagt tttaatatgt
     1681 tactggcagc aatgttttca attattttat tgttgttatt attctggctg atgaaaaccg
     1741 atttcggcat tgcaatgcgt gcaacaggca gcaatgagtt aatggttaaa gcgatgggca
     1801 taaatattaa tttaatgaaa attgcaggat tggcaatagc caatgcactg acagcattaa
     1861 gtggttattt aatggtgcaa tatcagggat ttgctgatat taatatgggt attggaattg
     1921 taatagccgg tttagcggct gttattctgg gcgaatcgtt tgcagttttt ttcaaatcat
     1981 cctctatatt tagtgcatta ataactgtct tattaggctc tgttttattt cgattgcttt
     2041 tggcatttgc attacttgcc ggtgtgcctc cgcaaatgct taaattactt acagcagttc
     2101 ttgtgttgct ggcagcagga gtttttaaat ttatttttaa gcctgcacaa tgattgaagt
     2161 ttcgcaaata agtaaaaagt tttttgatgg tgaaaagcct gtcattgctt tgcaacctac
     2221 atcactacaa attaaagtaa aagaatttgt gattattgtt ggtgccaatg gctccggaaa
     2281 atctacgttg ctcaatatta ttgcaggaac tatattatgc gatgcaggta aaattgctgt
     2341 taatggcaag gacattacgg cacttaaaga acatgaaaga agtcgtttta ttgccagagt
     2401 ttttcaaaac ccgcaatcgg gtactgcttc agatcttaca attattgaaa acttccggtt
     2461 agcggctttg cgaagtcatt caaagaagtt aagtataggc attgataaaa aatttgttga
     2521 tttcatcaga gaaagagttt cttggctggg actaggtctc gaagatcagt taaaccgaaa
     2581 gatgggagca ctgtccggag ggcaacgtca ggcactgaca gtagtgatgt caacaatgga
     2641 cgattttaaa cttctgctaa tggatgaacc cacggcagct cttgacccta aaagtgcaga
     2701 actcattatg cagctaacta cacgaatttt aagtcaaatg cctgtttcag tactaatggt
     2761 tacccatcag acaaaacagg ttattgatta cggaaacaga gttattcaga tgtcatcagg
     2821 acagataata agagatttag ataaagcatc aaaggagaaa cttaccctga atgaaattat
     2881 acattggttt gattaagcag aatagtccgg taataaaaaa tcgtctttag cgtagtgttt
     2941 ttcaaccttt cgccaaatta tatgttgttt tcattggtaa tttggggtag ttccattcat
     3001 tatttgtaag tttgcatcac tatttgctgt atggaaatta aagcaggaga gcttcttagt
     3061 agtattaaca cacctgacga tttaaggaaa attaaaccgg agcagcttga gcaggtttgt
     3121 aaggagttac gacagtttat tattgatatt gtttctgtta acggaggaca ctttggcgca
     3181 agccttggtg tggttgaatt aacggttgca cttcactatg tttttaatac acccaacgat
     3241 caattgatat gggatgtagg tcatcaggcc tatggtcata aaatcctgac cggcagacgc
     3301 gatgtttttc ataccaacag aatctataaa ggtattagcg gttttccaaa acgcagcgaa
     3361 agtgtttacg atacctttgg tgtaggtcat agctcaactt caatttctgc tgcgcttgga
     3421 atgagtgttg caagcagatt aaaagatgat aaaaaccgtc agcacattgc tgtaattggt
     3481 gatggtgcca tgacagcagg tatggcattt gaagcactta atcatggtgg tgtagaaaac
     3541 agtaaccttc tagttgtgct taacgacaac tgcatgagca ttgaccccaa tgttggtgca
     3601 ctgaaagaat accttactga cattaccaca tcaccaacat ataataaagt taaagacgaa
     3661 atctggaaag cattaggtgt catcagtaag tttggtccca atgcacaagc cattgctcaa
     3721 aaaatagaga atgcaatgaa atcggctgtg cttagacaaa gcaacttatt cgaatcgctt
     3781 cgctttcgtt atttcgggcc aatagatggg catgatgtga accatctgac caaagtactt
     3841 gaagatttaa aaaatattcc cggccctaaa attttgcact gtcttacagt aaaaggaaaa
     3901 ggatttaagc ttgcagagca agatcaaacc aaatggcatg caccggggtt gtttgataaa
     3961 ataaccggag agatttttaa gccgcaaaac aacacaccgc aaccaccaaa atatcaggat
     4021 gtatttggcc atacaatggt ggaacttgca gaaaaaaatt caaaaattgt tggtgtaaca
     4081 cctgctatgc catcggggtg ttcattaaat attatgatga aagcaatgcc tgatagagca
     4141 tttgatgtgg gtattgccga acaacatgct gtaacattta gtgcaggcat ggccacacaa
     4201 ggtcttattc cgttttgtaa tatttattcc tcatttatgc agcgtgccta cgatcaggta
     4261 gtgcatgatg ttgctcttca gaaattaaat gttgttttct gtctcgacag aggaggcctt
     4321 gccggtgctg acgggcctac acatcatggt gcatttgata ttgcctatat gcgatgcata
     4381 cctaatatga ttgttagcgc tccgatgaat gaagaagagt tacgcaatct gatgtacact
     4441 gcacaacatg ataacttagg accattttgt attcgttatc cgagaggtaa tggtgtgttg
     4501 acagaatggc gtacaccttt caaagcaatt cctgtaggaa caggtcgcaa attaagaggt
     4561 ggagaacaag ttgctatttt aaccttcgga catccgggta attttgcagt agcagcatgc
     4621 gatgagcttg ctaaagaaaa tatttatcct gcacattacg atttgcgttt tgtgaaacca
     4681 cttgaccaaa tgttgcttca cgaagtcttt gctaatttta aaaagataat tactgttgaa
     4741 gatggttgtc tgcaaggagg aatgggaagt gcagttgcag agtttatgat tgataatggc
     4801 tatgctgcag aaattgtcag actgggaatt cccgatcagt ttattgagca tggtgaacag
     4861 tctgaattat atgcagagtg tcatttcgat acgcaatcaa taatgaaaac tgttagacag
     4921 ttaattgctg cctcacatcc cggtattaaa tctctcaagt aatttttttt tctgatagtt
     4981 gagaaagcca ttcaatgttt cttgttgaag tatgtcgaat tttattttag tcatcatagt
     5041 tatctgtatg atgtaaataa agaaacccga cttctcaagt cgggtttcgg ggggacatac
     5101 acaatgaata atttaattac tcatcgactt ctgtttctgt tgcattgccg ggaaccgttt
     5161 ttactttttc ccattggcct gtgccccagt tatatttttc tatttcatat cgttcaactc
     5221 ctgtatccca tttatcatat ttagtccacc acagtctgct gtttgcatac tcccatttcg
     5281 attgtaacaa tatagaagaa ctgttgctgc ttaattgacc tgccatgtta caatcactga
     5341 ttacttcaat cttatagtaa tagttttgtt cattaacatt tacggcaaag tcttcgtagc
     5401 ttgtaacatt tgcagatacg gttgcaagca gtgagtagaa attgcctgtt gtgtccgtac
     5461 tacgataaat gttgtattgc attactcttt caggatgtaa tcttggctca gaccattctg
     5521 taagtacaaa gttgttgtta actactgttg aacgtacaat gattgcttgt tgctcctgca
     5581 aattgtaatc tggttttgca gctgctgtat cactgtatga tgtgtaagtt gttccacaaa
     5641 gatcataagc ttttattcta tagctgtaat catcaggaca tatcattgtt gaatcaaagt
     5701 aatacaatgt tgatgcaggg acagtggcta tcaccgcact gcttccattg ctcacttctg
     5761 ttctgctgat tt
//
LOCUS       DAOOOL010000146         5684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1519_length_5684_cov_65.898739, whole genome shotgun sequence.
ACCESSION   DAOOOL010000146 DAOOOL010000000
VERSION     DAOOOL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_5684_cov_65.898739"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3956)
                     /locus_tag="PKU82_10230"
     CDS             complement(<1..3956)
                     /locus_tag="PKU82_10230"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ83290.1"
                     /translation="MQKIFFALLLLFSLSRNAQSQCPTASFSLPDTVCSGIPLPLTNT
                     STGNGLTYQWDFCPQEIHGFTSYQYFRYNNYVLYTMTDMDLLKVGSGYYLITMDSIYN
                     NFVVGNIGNHLSNRPFDAIVHGNFTKLDCFDFIVEGDTNVYGLAAYSPTSTLYLMTFP
                     GGIQSTPVLTPLSSFSSVANPADIKIVHADNIYYAFIASKGNGNVTCLSFGNSMSNLP
                     LELYSFNITGSINLSDIDIVTTCAPPVGFVTDSKNGDLTKLSFSSGFNNAPSLSVYSG
                     TGSIGNNSVTITQEFESYFVCFTDTANKQLKWLQFNENNFANTPTVYTSPAFYFRPNQ
                     AESLTDSSITRFIVSDVPTLNFVDMLYFDSCYTKYSEDQNPSFSYLNTGWNKLSLTVS
                     DSNGYKSIAYDSVFVITGPKSQFNFTGNTCFNPNDSISFTDQSTSPSGAITGWLWDFG
                     DGTTSTQQNPQHAYTASGTYQVKLTITAGCEKDTIIPITIHALPIINFTYNLSCANSA
                     TTLTDQSSSAAGLQSWTWNFGDGSSSSQLQNPDHIFQTGGNYNVQLTVTDSSGCVDSL
                     VNIVQVKATPSAAFMALQTCLGDTVHLTNISTISDSTALAYFWDFGSGITSTQTDTVI
                     YFTSAGSYPTTLIAYSSQGCSDTLNQNIVISTPPSVNFGFPSSNCQYNAINFSDSTVG
                     FNLSSWQWDFGDGDTSSLQNPTHVYNNAGNYTVQLTVSSGNDCAASYSQTINILAGPS
                     AAFLTGNGCQGVSTNFTDQSTVSSGSSIVAWQWYFGDGDSSQTQNPSHIYAVPGSFNA
                     LLRVTSNNGCQDTTSQPIQVFENPIAGFYVQPLRCANTEIYFLDTSYIGNGNITQWQW
                     QFSNGTTSSLPNPFALFNNAGNATATLTVITSDGCSNSKTFNFTVRPQPDFTISHNGV
                     CKGNQTAFNYYPNGNISAYAWTWYFGDNTYAFTPNATHLYTTIGTYPISMAVTDSFGC
                     QKTIYDTLTIYPVPNTAFITNGICQNSPVDFVNQTDSVSTQISGWQWSFGDGNTSSQH
                     SPQHIYNGTGNYTVSLIAYAAGGCNDTIVSVVNMKPVPVIAFTIVPPTGAPGNTIQVL
                     NNTTGATSYSWDFGDGTAIDPNESPSHVYSDTGSYFITLTASSIFGCEVQMQKPFDVI
                     VPYVDLWLKSLSYNINDNYLNLSALLSNAGNTTVNNFELQIQSEGKGSFIENANETIV
                     YGTDKTYVLHTSISVNPLKLPDYICVTIKNVNGSSDKNPDNNEKCISLMPENGSLTVS
                     PNPIDDLLSIHLNLPVSSEIKLVLYDAAGKKLTTLYNGPSDAGLKIFSFKLPYLSKG"
     gene            4008..4592
                     /gene="rdgB"
                     /locus_tag="PKU82_10235"
     CDS             4008..4592
                     /gene="rdgB"
                     /locus_tag="PKU82_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOZ83291.1"
                     /translation="MTELIFCSNNEHKLQEIRKMLPFGYILQSLSEAGIQSNIDETGT
                     TFLENAFLKANAVSLLTKKNCFADDSGLCVAALNNAPGVYSARYAGNEVTDADNRRKL
                     LSDLSGIQQRNAYFIAVICLIWKGKAFYFEGRIDGMITHIEQGNSGFGYDPVFIPDGY
                     KITFAEMSPDEKNKISHRQIAVSKMIAFLRNSKV"
     gene            complement(4582..>5684)
                     /locus_tag="PKU82_10240"
     CDS             complement(4582..>5684)
                     /locus_tag="PKU82_10240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ83292.1"
                     /translation="AYIQSGDIEYINRRRKRDGQKALEPIYEIEDVEKAMKLFAPVAV
                     NKRFHYDDEISFEFTDAGHILGSVSVHLFITEKGNTKQITFSGDVGRFNDLILKAPAS
                     FSQADYILCESTYGNRLHDQSTDARQKLLQIVNKTCVEKKGKLIIPAFSLGRTQEIIY
                     TLDRFKTENKLPSIPVFVDSPLAIDATNIMRKHSEFFNSDLVNYLHVDDDPFGFSNLK
                     YVRKVEESKKINDLKGPCIIISASGMIEAGRIKHHIKNNINDSRSTILIVGYCTPESI
                     GGHLMRGDKTIKIFGNEYLVKASVEVISSFSAHADYLELIKYLSCQDTSAVKKLFLVH
                     GEYEVQKEFKEKLNEVGFKNVEIPEELQSFKL"
BASE COUNT         1774 a          980 c         1104 g         1826 t
ORIGIN      
        1 cccttgctca gataaggaag tttaaaactg aaaattttta aacctgcatc agaaggacca
       61 ttatataatg ttgtcagttt tttgcctgca gcatcgtaaa gtaccaattt aatttctgaa
      121 cttacgggta aatttaagtg aatagaaagt aggtcatcaa ttggattggg gctcacagtc
      181 agacttccat tctccggcat tagtgaaata catttttcat tattgtctgg gtttttatcg
      241 ctgctcccat ttacattttt aattgtcaca caaatgtagt cgggcagttt tagcggattt
      301 acagatatgc ttgtgtgcaa tacataggtt ttgtcagttc cataaacgat tgtttcatta
      361 gcattttcga taaaagaacc tttgccttcc gattgaattt gtaattcaaa attatttacc
      421 gttgtatttc ctgcattaga aagcaatgca gatagattta aataattatc gttgatatta
      481 taactcaaag attttagcca aaggtcaaca taaggaacaa taacatcaaa aggcttttgc
      541 atttgcacct cacacccaaa aatactggat gcagttaatg tgataaaata acttcctgta
      601 tcactataaa catgcgaagg gctttcatta ggatctattg ctgtgccatc gccaaaatcc
      661 caggaataag aagttgcacc tgttgtattg tttaaaacct gaattgtatt cccaggagct
      721 cctgttggcg gcacaatggt aaatgcaatt accggtactg gtttcatatt aactactgaa
      781 acaatggtgt cgttacatcc tccggcagca taggcaatca aagatactgt gtagttgcct
      841 gtaccgttat aaatatgttg aggtgaatgt tgtgaagatg tattgccatc gccaaaactc
      901 cattgccatc cgctaatctg tgtactaacg ctgtctgttt ggtttacaaa atcaaccgga
      961 gaattctgac aaattccatt ggtgataaat gctgtattcg gaacaggata aattgtcaaa
     1021 gtgtcgtaaa tagttttctg acaaccgaaa gaatctgtta cagccattga tataggatat
     1081 gttcctattg ttgtatataa gtgtgttgcg tttggagtaa aagcataggt gttgtcacca
     1141 aaataccatg tccatgcata ggcagatata tttccattcg gataataatt aaatgcagtt
     1201 tgatttcctt tacaaactcc attatgcgaa atggtaaaat caggctgtgg tcttacagta
     1261 aaattaaatg ttttgctgtt gctgcaacca tctgatgtaa tcactgttaa tgttgcagtg
     1321 gcatttcctg cattgttaaa taaggcaaat ggattgggaa gactgcttgt tgtgccattg
     1381 ctgaattgcc attgccattg agtaatatta ccattgccaa tatagctggt gtcaagaaaa
     1441 taaatttctg tattggcaca acgtagcggt tgtacataga agcctgcaat tggattttca
     1501 aaaacctgaa taggttgtga tgttgtgtct tgacaaccat tgtttgaagt tacacgcagt
     1561 aatgcattaa aactaccggg aactgcgtaa atgtgtgaag ggttttgtgt ttgcgaagaa
     1621 tcgccatcac caaaatacca ctgccacgcc acaatactgg aaccactgct tacagttgat
     1681 tgatctgtaa agttagttga tacaccctga cagccattgc ctgttaggaa tgctgctgaa
     1741 ggtcctgcaa gaatattgat agtttgtgaa taggatgctg cacaatcgtt acctgaagaa
     1801 actgtaagct gaacagtata attacctgca ttgttgtaaa cgtgcgttgg gttttgaaga
     1861 gaagaagtat caccatcacc aaaatcccat tgccatgatg aaaggttaaa accaacagta
     1921 ctatcactaa aattaatggc attgtattga cagttgcttg atggaaaacc aaagtttaca
     1981 gaaggcggtg ttgaaataac tatattctga ttgagtgtgt ctgaacaacc ttgtgatgag
     2041 tatgcaatta atgttgtcgg gtagctacct gccgaagtaa aataaattac agtatcagtt
     2101 tgtgttgaag taataccact gccaaaatcc caaaaatagg cgagtgcagt actatcacta
     2161 attgttgata tgttagtgag atgcaccgta tcgccaagac atgtttgcaa tgccataaat
     2221 gcagcagagg gtgttgcttt tacctgcaca atgttgacta atgaatccac acatcctgag
     2281 ctgtcagtta ctgtgagttg cacattataa ttaccccctg tctgaaatat atgatcagga
     2341 ttttgcagtt gactgctact gcttccatca ccaaaattcc atgtccacga ctgcaaacct
     2401 gctgccgatg aagactgatc tgttaatgtt gttgcagaat tggcacacga taaattataa
     2461 gtaaaattta taatcggtag tgcatgaata gttattggga ttatcgtatc cttttcgcat
     2521 cctgcagtga tggttagttt aacctgatag gttcctgatg ctgtatatgc atgttgtggg
     2581 ttttgttgtg tggaggttgt tccatcacca aaatcccaca accatcctgt aattgctccg
     2641 gagggcgagg ttgattgatc tgtaaaagaa atactatcat taggattaaa gcatgtattg
     2701 cctgtaaagt taaactgtga ttttggaccg gtgataacaa atacactatc ataagcaatg
     2761 gatttatatc cgtttgaatc agaaacagta agtgataatt tattccatcc tgtatttaaa
     2821 tagctgaaag aaggattttg atcttcactg tattttgtat aacaactgtc aaaataaagc
     2881 atgtcaacaa agttcagtgt tggaacatcg ctcactatga atcgggtaat tgatgaatcg
     2941 gtcagagatt cagcctgatt tggacgaaag taaaaagcag gtgatgtata aacggttggc
     3001 gtattggcga agttattttc attaaactgc agccatttca attgtttatt tgctgtgtct
     3061 gtgaaacaaa caaagtaact ttcaaattcc tgggtaattg taacagagtt gttaccaatt
     3121 gaacctgtac cggagtaaac agacagagaa ggggcattat taaatccact actaaatgat
     3181 agtttggtta gatcaccatt tttggaatct gtaacaaatc ctacaggtgg agcgcaagta
     3241 gttacaatat caatgtcaga taaattaatg cttcctgtta tgttgaagct gtataattct
     3301 aagggtaaat tcgacattga gttgccaaat gaaagacagg taacattccc gttcccttta
     3361 cttgctataa atgcatagta aatattgtca gcatgaacta ttttaatatc tgcagggttt
     3421 gctactgagc tgaatgatga caaaggtgtt aataccggtg ttgactgaat tcctccgggg
     3481 aaagtcatca agtataaagt gctggtagga gagtaggcag ctaatccata aacattggtg
     3541 tcaccttcaa caataaagtc aaaacagtca agcttggtaa aattgccgtg tacaattgca
     3601 tcaaaagggc gattagataa atggttgccg atattaccaa ctacaaagtt gttgtaaatg
     3661 ctatccatcg taatcagata atatccactt ccgactttta gtaagtccat atcagtcata
     3721 gtataaagca cataattgtt gtatcggaaa tattgatacg atgtgaaacc atgtatttcc
     3781 tgaggacaaa aatcccattg ataggtcagt ccgtttcctg ttgaagtgtt tgttaaagga
     3841 agtggaatgc cgctacagac agtatcaggt aatgaaaaag aagctgtagg gcattgcgat
     3901 tgtgcatttc tgcttagact gaataacagc aataaagcaa aaaatatttt ctgcatttta
     3961 aggtggtttt taaaacaaag taaatgtata tatttttgac ttaaaaaatg actgagttaa
     4021 tattttgttc caacaatgag cataaattac aagagatacg taaaatgctg cctttcggtt
     4081 acattttaca gtcgctttca gaggcaggaa ttcaatccaa tattgatgaa accggtacaa
     4141 cctttcttga aaatgccttt ctgaaagcta atgccgtttc tttattgact aagaagaatt
     4201 gttttgcaga tgacagtggt ttatgtgttg ctgcattgaa caatgcaccg ggagtttatt
     4261 cggcacgtta tgcaggaaat gaagttaccg atgctgataa cagaagaaag ttgttgtcgg
     4321 acctttcagg aatacaacaa agaaatgcat attttatagc agttatttgt ttgatttgga
     4381 aaggtaaagc tttttacttt gaaggcagaa tagatgggat gataacccat atcgaacaag
     4441 gtaattcagg atttggctac gaccctgttt ttattcctga tggatataaa atcaccttcg
     4501 cagaaatgtc tcctgacgaa aaaaataaaa tcagtcacag gcaaattgcc gtaagtaaga
     4561 tgatagcttt tttgagaaat tctaaagttt aaatgattgc agttcttccg gaatttcaac
     4621 attcttaaaa ccaacctcat ttagtttctc tttaaactct ttttgtactt catattcgcc
     4681 atgtaccaga aaaagttttt taactgctga tgtatcctga caagaaagat attttatcaa
     4741 ctctaaataa tcggcatgtg cactaaagga tgaaattact tcaacacttg cttttacaag
     4801 gtattcattt ccaaaaattt taattgtttt atctccacgc atcaaatgcc cgccaattga
     4861 ttcaggtgtg caataaccaa caataagaat ggtgcttcga ctatcattga tgttgttttt
     4921 aatatgatgt tttattcttc cggcttcaat catacctgat gcagaaatga tgatgcaagg
     4981 tcctttcaga tcattaatct ttttcgactc ttcaactttt cgtacatatt taagattact
     5041 aaagccaaag ggatcatcat caacatgcag gtagttcact aaatcggaat taaaaaactc
     5101 actgtgtttg cgcatgatat ttgtggcatc aattgcaagt gggctgtcaa caaaaacagg
     5161 tatagaaggc agtttgtttt ctgtcttaaa acggtctaat gtgtatatta tttcctgagt
     5221 ccggccaagg ctgaatgccg gaattatcaa tttgcctttt ttttcaacac aagttttatt
     5281 gacaatttgt aataatttct gtcgtgcatc tgttgactgg tcgtgaagtc tgtttccata
     5341 agtagattca cataaaatgt agtctgcctg cgaaaatgat gcaggcgctt ttaggattaa
     5401 atcattaaaa cgacccacat ctccactaaa agtaatttgt tttgtattgc ctttttcggt
     5461 aatgaataaa tgcacactga cactacctaa aatatgtcct gcatctgtaa attcgaatga
     5521 aatttcatcg tcataatgaa agcgtttgtt gactgccaca ggggcaaaga gtttcatagc
     5581 tttttctaca tcttcaattt cataaatagg ctcaagtgct ttttgtccat ctctttttcg
     5641 tcttctgttg atatattcga tatcaccact ttgaatatag gcac
//
LOCUS       DAOOOL010000147         5678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1521_length_5678_cov_133.649475, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000147 DAOOOL010000000
VERSION     DAOOOL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_5678_cov_133.649475"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1404)
                     /locus_tag="PKU82_10245"
     CDS             complement(139..1404)
                     /locus_tag="PKU82_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOZ83293.1"
                     /translation="MSELPDNSVHLAITSPPYWQLKDYGTENQIGFHDSYENYINNLN
                     LVWKECYRVLHDGCRLCVNIGDQFARAVYYGRYKVIPIREEIIKFCENIGFDYMGAII
                     WQKVTTSNTTGGGVQMGSYPYPRNGILKLDYEFILVFKKLGDSPKPTKEQKELSKMSA
                     EEWNTFFAGHWNFAGARQNNHIAMYPEELPRRLIKMFSFVGETILDPFVGSGTTALAA
                     KNTERNSVGFEINPAFIQIIKEKLEVHQLDLNATTYEFVKQKKINLDFEKEIQKLPYI
                     FKDPHILDKKIDVKKLQFGSKIDKDSKTKREEMFTVKEVLSPEKIRLNNNMTVKLIGV
                     KEDPNINGKATSFLQEKIKGKRVFLKYDNVKHDSENNLLCYLYLENKTFINAHLIKSG
                     FVKVDIGVEFKYKEKFQSLFNEVHAQADK"
     gene            complement(1648..2952)
                     /gene="sufD"
                     /locus_tag="PKU82_10250"
     CDS             complement(1648..2952)
                     /gene="sufD"
                     /locus_tag="PKU82_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOZ83294.1"
                     /translation="MNSSVDTLSVTNETSIAEDNIPVDGLQKLRLAALNKFLEKGFPD
                     SKHEEWKYLNLKPISETKYNHRVVSSPLKASDLEQFYDAGNDTVNLVFINGRYCSEFS
                     GHQSHKHPFSIYSLESREAELYLETLRNSEDEPFHALNTAFHQGGIMIHVPDDCRMPM
                     TIHLLYVNDSQNEAVAAHPKCIVVAGKNSAFQVIATYHSLNNFPSLTNTVTEFLIGEN
                     AVVEFDIKQNENSNANHISRVYARVQRKAVFDIFTVTMGGALVRNNLEIILSEPNACA
                     HLNGLTVADGYQVVDNHTVVDHASPNCESNQLYKNILDDHSQGVFNGKIFVRKDAQKT
                     NAFQSSKNVLLSNTAVMNAKPQLEIFADDVKCSHGATTGQLDEDALFYFRSRGIGERD
                     ARALLNYAFASDVIEKINNDALKSNLLKLLSQKLNTAVDFEE"
     gene            complement(2964..3716)
                     /gene="sufC"
                     /locus_tag="PKU82_10255"
     CDS             complement(2964..3716)
                     /gene="sufC"
                     /locus_tag="PKU82_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOZ83295.1"
                     /translation="MLNIKNLHASVEGREILNGLNLQVNPGEVHAIMGPNGSGKSTLA
                     SVLAGREAYEVNDGEILYHGENLIELSPEERARKGIFLAFQYPIEIPGVSNANFLKTA
                     VNEVRKSKGMDVLDSKDFLKLMKDKMQLVKMDKSMTQRSVNEGFSGGEKKRNEIFQMA
                     MLEPELCVLDETDSGLDIDALRIVSDGINALRDGKRSFIVITHYQRLLDYIVPDFVHV
                     LYKGKIVKSGPKELALELEEKGYDWIKEEVEI"
     gene            complement(3762..5207)
                     /gene="sufB"
                     /locus_tag="PKU82_10260"
     CDS             complement(3762..5207)
                     /gene="sufB"
                     /locus_tag="PKU82_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOZ83296.1"
                     /translation="MSEDLKIIDEVTSGEYKWGFVSNIESENAPKGLSEDIVRFISAK
                     KKEPEWMLEFRLKAFRHWQKMQEPQWANVNYPKVDFQDIIYYSAPKPKKQLNSLDELD
                     PEIKATFDKLGISLLEQKRLAGVAVDAVIDSVSVKTTFRQELSKLGIIFSSFSEAVQE
                     HPELVKKYMGSVVPYTDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINSAGTG
                     QFERTLIIADEGAYVSYLEGCTAPKRDENQLHAAVVEIYAHTDAQVKYSTVQNWYPGD
                     KDGRGGIFNFVTKRGLCAGERSKISWTQVETGSAVTWKYPSVILKGDYSTGEFYSVAL
                     TNNYQQADTGTKMIHIGKHTRSTIISKGISGGRSNNSYRGLVRIGKQAEKARNFSQCD
                     SLLLGDKCGAHTFPYLECANPTAKVEHEATTSKVGEDQIFYCNQRGISTEDAIGLIVN
                     GYCKEVFNQLPMEFAVEAQKLLAVSLEGSVG"
     gene            complement(5279..5605)
                     /locus_tag="PKU82_10265"
     CDS             complement(5279..5605)
                     /locus_tag="PKU82_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOZ83297.1"
                     /translation="MITVTEKAKSKALSLMQAENKPADAFIRVGVEGGGCSGLTYMLV
                     FDTELKPEDKVFEDKGIKIVCDRKSFLYLVGTELDYTDGLNGKGFQFNNPNASRTCGC
                     GESFAV"
BASE COUNT         1662 a         1178 c          872 g         1966 t
ORIGIN      
        1 ttttatacct cgtaattgtt gactataaca gcaattaaac ctatctttgc accaccttta
       61 ttcggccttg ataattgata ggtctgtgtt gtataattaa gaaccttttc ctttttaccg
      121 aattccttcg agaaacgttt atttatcggc ttgggcatga acttcgttaa ataagctttg
      181 aaatttctct ttgtatttaa attctacccc gatatccacc ttcacaaaac cacttttaat
      241 taaatgcgca ttgataaaag ttttgttttc aagatataga taacacagta agttattttc
      301 actatcgtgc tttacattat catatttcaa aaacacacgt ttccctttta ttttctcttg
      361 taagaaagaa gttgctttac cattaatgtt tgggtcttcc tttactccaa taagctttac
      421 agtcatgtta ttgtttaatc gaattttttc aggactaaga acttctttaa ctgtaaacat
      481 ttcttctcgt ttagttttac tgtccttatc aatcttagaa ccaaattgaa gtttttttac
      541 atctatcttt ttgtcaagaa tatgaggatc tttaaaaata tatgggagtt tttgaatttc
      601 tttttcaaaa tctaagttga tttttttttg cttaacaaac tcataagtag tagcatttaa
      661 atctaactga tgtacttcca atttttcttt tatgatttga ataaatgcag gattaatttc
      721 aaaaccaaca gaatttcttt cagtattctt tgctgccaaa gcagttgttc cacttcctac
      781 aaaagggtca agaatggttt cgccaacaaa cgaaaacatt ttaattaatc ttcttggtaa
      841 ttcttcgggg tacattgcaa tatgattgtt ttgtcgcgct cctgcaaaat tccaatgccc
      901 ggcaaaaaag gtattccatt cttcagctga cattttagaa agttcttttt gctccttagt
      961 aggttttggg ctatcgccca attttttaaa aacaagtata aactcgtaat ctagttttaa
     1021 aattccattt ctcggataag gataagaacc catttgaaca ccaccacctg ttgtgttcga
     1081 agtagtgact ttctgccaga taattgcacc catgtaatca aatcctatat tttcacagaa
     1141 cttaataatc tcctcccgaa ttggtatgac tttatatctt ccataataaa cagctcttgc
     1201 aaattggtca ccaatgttca cacataacct acaaccgtca tgcaaaacac gataacattc
     1261 tttccatact aaattcaggt tattgatata attttcataa ctatcatgaa agcctatctg
     1321 attttctgta ccatagtctt ttaactgcca ataaggtggt gatgttattg ccaaatgaac
     1381 agaattatca ggcaactcag acatgtgtct gctatctccg ctaattattt tatgattcgt
     1441 cttcaagttt ctaacttaca agtttatctt ggtcaaattt aaacaaaatg ttaagctaaa
     1501 cttataagtt ttcttaaatt ttctgcctga atcgtaatag attcacttca taacaatcaa
     1561 atggaaatta tacaaacatt tctacactaa atgtaagttc ttatttgatg ccaatagtcc
     1621 cgtttaaatt acactaataa acttaaacta ctcctcaaaa tcaaccgctg tatttagttt
     1681 ttgcgataac agttttaaca agtttgattt aagtgcatcg ttattgattt tctcaataac
     1741 atcactggca aatgcataat tcagcaaagc acgagcatca cgttcgccaa taccacgcga
     1801 acggaaataa aacaatgcat cttcgtccaa ctgacctgtg gttgctccat gtgaacattt
     1861 tacatcatca gcaaaaattt caagttgtgg ctttgcattc atcacagcag tgttactcaa
     1921 caacacattc ttactgctct gaaaagcatt ggtcttctgt gcatctttcc gaacaaatat
     1981 ctttccgtta aaaactccct gcgaatgatc gtctaagata tttttgtaca attgattgct
     2041 ctcgcagttt ggacttgcat gatctaccac tgtgtgatta tctaccacct gatagccatc
     2101 agcaacagtg agtccgttaa gatgagcaca ggcatttggt tcacttaaaa taatttcgag
     2161 gttgttacga actagtgcac cacccatcgt aacagtaaaa atatcgaaca cagctttacg
     2221 ttgtacacgg gcataaactc tgctaatatg gttggcatta ctgttttcat tctgcttaat
     2281 atcgaattca acaactgcat tttcaccaat aaggaactct gttactgtat tggtgagtga
     2341 tggaaaatta ttgagtgaat gataagttgc aattacctga aatgctgaat tttttcctgc
     2401 aacaacaata cattttggat gtgctgcaac ggcttcattc tgcgagtcgt taacatatag
     2461 gagatgtatt gtcattggca tacggcaatc atcaggaaca tgaatcataa taccaccttg
     2521 atgaaaggct gtatttaatg catgaaaagg ttcgtcttct gaattacgca aagtctctaa
     2581 atacagttct gcttctctgc tttcgagcga ataaatactg aaaggatgtt tgtgactttg
     2641 atgtcctgaa aactcagaac aataacgtcc gtttataaaa accaaattca cagtatcgtt
     2701 gcctgcatcg taaaattgct ccaagtctga agctttcagt ggcgatgaca ctacacggtg
     2761 attatattta gtttcactta taggtttcaa attaagatat ttccactctt catgctttga
     2821 atcaggaaat cctttctcta aaaatttatt taatgctgcg agtcgcaatt tttgcaatcc
     2881 gtcaacaggg atattatctt cggcaatgct tgtttcgtta gtcacagaaa gtgtatcaac
     2941 agatgagttc acaataatca ggtttaaatt tcaacttctt cttttatcca atcatatcct
     3001 ttttcttcca gctcaagagc aagttctttt ggtccgctct taacaatttt tcctttatac
     3061 aaaacgtgta caaaatcggg aactatgtaa tcgagcaatc gctgatagtg tgtaataaca
     3121 atgaaagaac gtttgccgtc acgcaatgca ttgataccat cggatacaat cctaagtgca
     3181 tctatatcta gacctgaatc ggtttcatcg agtacacata actcaggctc aagcatggcc
     3241 atctgaaaaa tttcattgcg tttcttctct cctccgctga atccttcatt aacactgcgt
     3301 tgtgtcatgc ttttatccat cttcacgagc tgcattttat ctttcatcaa ctttaaaaag
     3361 tcttttgaat cgagtacatc catacctttc gacttacgca cttcgttaac tgctgttttc
     3421 agaaagttgg cattactcac accgggaatt tcaatcggat attgaaatgc cagaaatatt
     3481 cctttacggg ctctttcttc aggagaaagt tctattaggt tctcaccatg atacaatatt
     3541 tcaccatcat taacttcata cgcttcgcgt cctgcaagta ccgatgcaag cgtacttttt
     3601 cctgaaccat taggtcccat aatggcatgc acttctcccg gatttacctg aagatttaat
     3661 ccgtttaata tttctctacc ttcgactgag gcatgtagat ttttaatgtt taacatactt
     3721 tctaaaaaac tttattcttc ttatctaact atatttaatt tttatcctac tgatccttct
     3781 aatgaaaccg ctaatagttt ttgtgcttct acagcaaact ccatgggcaa ctgattaaac
     3841 acttctttgc aataaccatt tactataagt ccaattgcat cttctgtaga gatacctcgt
     3901 tgattgcaat aaaatatctg atcttctcca acctttgatg ttgtagcctc atgctcaact
     3961 ttagctgtcg ggtttgcaca ctccaaatat ggaaatgtat gtgcaccaca tttatcaccc
     4021 aacaacaaag aatcgcattg tgaaaaattt cttgctttct cagcctgttt accaatacgt
     4081 accaatccac gataactatt gttactgcgg cctccgctta ttcctttact gatgatggtg
     4141 ctgcgtgtat gtttaccgat atgaatcatc tttgtaccgg tgtcggcctg ctggtagttg
     4201 ttggttaatg caacagaata gaattcacca gttgaatagt caccttttaa tatcacactt
     4261 ggatatttcc atgtaacagc actacccgtt tcaacctgtg tccaactgat tttagaacgt
     4321 tcgcctgcac aaagtccacg cttggtaaca aaattaaaaa tacccccacg accatctttg
     4381 tcaccgggat accagttttg tacagtgcta tactttacct gtgcatcggt atgtgcataa
     4441 atttctacta ctgcagcatg caattgattc tcgtcacgct ttggtgctgt gcaaccttca
     4501 agatagctaa cataagcacc ttcatcggca atgattagtg ttctttcaaa ctgacctgtg
     4561 cctgcagagt ttatacggaa ataggtactc aactccattg ggcaacgtac acctttgggt
     4621 atataacaaa aagagccgtc actgaaaacg gcactgttta atgcagcata aaaattgtcg
     4681 gtatagggta ctacactgcc catatatttt tttaccagtt ccggatgttc ctgaacagct
     4741 tcactgaatg atgaaaatat tattcccagc ttacttagct cctgacggaa agtagttttt
     4801 acgctaacac tgtctatcac tgcatcaaca gctacacctg caaggcgttt ttgttctagt
     4861 aatgaaattc caagtttatc aaacgtagct ttgatttcag gatcaagctc gtctaagctg
     4921 ttgagttgct tcttgggctt gggtgccgag taataaatga tatcctgaaa atcaactttc
     4981 ggatagttga cattagccca ctgtggttcc tgcatttttt gccagtgacg gaatgctttc
     5041 agtcggaatt caagcatcca ctcaggttct tttttcttgg ctgaaataaa tcttacaata
     5101 tcttcagaca atcctttggg ggcattctct gattcaatgt tacttacaaa tccccactta
     5161 tactcgccac tcgttacttc atctattatt tttaaatctt cgctcacttt agtttaaatt
     5221 tggttattta gactttttct aaaaaaaatt attagtcaaa aaaaattatc tactcatttt
     5281 agaccgcgaa actttctcca cagccacagg ttcttgatgc atttgggtta ttaaactgaa
     5341 accctttgcc atttaaacca tctgtataat caagttctgt acctactaaa tacaaaaacg
     5401 attttctgtc gcacactatt tttatccctt tatcttcaaa cactttatct tccggtttta
     5461 gttctgtatc aaaaaccaac atataggtta atccagaaca tccgcctcct tctacaccaa
     5521 cgcgaataaa tgcatcggca ggtttgtttt ctgcctgcat taaacttaag gcttttgatt
     5581 tggctttttc agttactgtt atcattttca agtacttata aaagtggtgc aaagataggt
     5641 ttaattgctg ttatagtcaa caattacgag gtataaaa
//
LOCUS       DAOOOL010000148         5662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1526_length_5662_cov_37.226859, whole genome shotgun sequence.
ACCESSION   DAOOOL010000148 DAOOOL010000000
VERSION     DAOOOL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_5662_cov_37.226859"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..2256
                     /locus_tag="PKU82_10270"
                     /pseudo
     CDS             197..2256
                     /locus_tag="PKU82_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
     gene            complement(2301..2684)
                     /locus_tag="PKU82_10275"
     CDS             complement(2301..2684)
                     /locus_tag="PKU82_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOZ83298.1"
                     /translation="MKKSYIIAMVLIAVAFAAIISTVSDSGTYANFTEASSHEGKVFH
                     VVGKLNRDKPYEYHPEKNANLFTFYLYDNNGNERKVLLNKAKPQDFDKSEQIVIIGKM
                     KGEEFIASDILLKCPSKYADEAQKS"
     gene            complement(2694..2921)
                     /locus_tag="PKU82_10280"
     CDS             complement(2694..2921)
                     /locus_tag="PKU82_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOZ83299.1"
                     /translation="MEFSRKLKILFSVLMLVATNVFAQTESALDETFRSNGKIYVVVA
                     VLAVVLTVLFIYLISIDRKLKKTEDKLKNKN"
     gene            complement(2900..3556)
                     /gene="ccsA"
                     /locus_tag="PKU82_10285"
     CDS             complement(2900..3556)
                     /gene="ccsA"
                     /locus_tag="PKU82_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOZ83300.1"
                     /translation="MHNVWKIACVALLYFTLFAGFLMPVPELPILNESIRNLYFHVTM
                     WFAMIIHMVVTIVYSIRYLSGGKIQYDLKAEQYAITGMVFGIAGLITGMIWARFTWGA
                     FWVNDTKLNGAAAAMLMYAAYFILRQSTEDETKRAKLSAVYLVFAFPLMMAFIYILPR
                     MTDSLHPGNGGNPAFGQYDLDNQMRLVFYPAIIGWTLLGVWITSIRIRIKKLWNSHEN
                     "
     gene            complement(3593..4249)
                     /locus_tag="PKU82_10290"
     CDS             complement(3593..4249)
                     /locus_tag="PKU82_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOZ83301.1"
                     /translation="MFNQIGLIIYKEWKLEWRNRAAMAGLMVFILSGVYISYLSFKKI
                     VDVSAWNALFWLLILFMAVNALAKSFMAESRGLQLFYYSFLNPYAVILGKIIYNGLML
                     FLFGIITFFIYGILLGNPVQDIGMFILTVLLGCFGISSVLTMISAIASRAGHQSSLMA
                     ILGFPVLLPLLIVLIHLSKNAIDGIDFSVNLKYLTMLTALNLLVPSLAYILFPYLWRE
                     "
     gene            4465..>5662
                     /locus_tag="PKU82_10295"
     CDS             4465..>5662
                     /locus_tag="PKU82_10295"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ83302.1"
                     /translation="MKKVLLTLLAATTIISANAQRKAGINIKEQPTMTQRQCGTGELP
                     LEYETWMAAKMKEPNANKMQVVYNIPVVVHVIHNGTNVGVNQNISDAQINSQIAVLNE
                     DYRRQNPDTTSTPSVFGAVAADCEINFCLAQRDPSGNPTNGIDRINRNTQGWTAPPYT
                     KTYIDGTIKPATIWDPTQYLNIWVVPDYSASGNQLLGHATFPLNSTLTGLTGSYGTAT
                     NDGVVVWYKSFGRVGTLDALYNKGRTATHEIGHWLGLRHIWGDANCGNDYVSDTPTQQ
                     TANYGGQNPPYPCPSFPLVTCNNGPNGDMWMNYMDYCYDKCLNMFTAGQKSRMQIAMA
                     NGTYRAPLATSLGCSAPSAGPTAQFTANTTTIAPGGSVNFTDQSSNSPTSWSWSFPGG
                     TPSSSTA"
BASE COUNT         1884 a         1054 c         1054 g         1670 t
ORIGIN      
        1 aaagcactta tgagcagttt atagacaaat atccttcaag cgtattcatt gctgaagcac
       61 gaaggcatta cagattactc agaggtgaca aaataaactg actttgttac aaaacttaca
      121 ttccacaatg ctttgatttt gttattttgt gccacattta aaaccgggct ttaatccggt
      181 gaagttaaat ttgactatgc aacaaggttt gagaattatt ttaactgcca tcatattatt
      241 tttcggcaca ggcagtaatg cgcagatttt tgaaggtgcc aagtggagtt tcagagttga
      301 acagacagac agttgtgaag ctacgctgat attaaaagct gtaacggtag ataaatttca
      361 tatctattct caaaccatca atggtgctga tgggcctata cccacaagtt ttacttttaa
      421 tcattctcct gaatacacta cagttggcaa agtaaaagaa ggtcatgccg aagaagtata
      481 tgatcagcag tttcagatga aactgaaatt ttttcatggt gaaacagagt ttaagcagaa
      541 aataaaatca acacaagaaa aaaattcagc attacaggtt ttgtaacctt tatggcttgt
      601 gatgatgaaa aatgtctgcc accaacagat gttgacttta aatttgattt gaatgatggc
      661 aagaaaaaat cgtgtgctac aactgaactt gcaattcctg taaacaatac aacacctgtg
      721 gttacagaca ctgcacaggc agtgatgcca ccggaacata ttgattcagc aacggcagca
      781 gcacaaaacg acagtgtatt tacagaagta gcacaagtat cagaacttga accgggctgc
      841 ggtgatggct caacatcaac tgccacagat aaaagcatgc tcggaatttt tattgcagga
      901 tttttaggtg gctttttagc tttacttaca ccttgcgttt ttccgatgat acctatgaca
      961 gtaagttttt ttacaaagcg cagtggttca cgtcagaagg ggttgatgaa tgcattaatc
     1021 tactctattt ctatcattat tatttatgta ggtcttggat ttttagtcac ggttacatta
     1081 ggtagtgatg cactcaacga tatggcaagc aatgtctttt ttaatatgac attttttatt
     1141 gtgttcctga tttttgcttt ttcattcttt ggtgcatttg aaattgtatt accaagctgg
     1201 ttagttaaca aagccgacag cgcctccgac cgtggcggat tgataggaat tttctttatg
     1261 gcatttactt tgacattggt ttcattctca tgtacaggac caattatcgg cacactgtta
     1321 gtagaagctg cacacaatgg aagttattta ggtccaatta taggaatgac aggatttgct
     1381 ttggcacttg cattgccatt tggacttttc tctgcatttc cggggtggtt aaactccatg
     1441 cctaaatcag gtggatggct taatactgta aaagttacac tcggttttat tgaattggca
     1501 ttggcattta aatttttatc gaatgttgac ctcgcttatc actgggaatt tttgaagcgt
     1561 gaaattttta ttgccatctg ggtaatcatt tttggtatgc ttggctttta tttattaggc
     1621 aaattaaaat tttcgcatga cagcgatgta aaacatatca ctgtaccccg actgatgttt
     1681 gccatcatat cattaacatt tacattgtat ctgattccgg gaatatgggg cgcaccatta
     1741 agaatgatca gcggctttcc tccacctgaa ttttataaag aatgggtaac agaaaaatca
     1801 agcaatcatt gtccacataa cttaaactgc tatcatgatt acgatgcagg tatggcatac
     1861 gctaaaactg taggcaagcc ggtgatggtt gattttacag gatggagttg tgttaactgc
     1921 cgtaaaatgg aagacaatgt gtggagtgat cctgaagtgc tgaaaaggct ttcggataaa
     1981 tatgtgctca tttcacttta tgttgatgac aaggagcaac taccggaaga caaacaatat
     2041 gtttctgcaa caacaggtaa aaaaataaga actatcggta ataaatggag cgatttacaa
     2101 accaatgttt acaaaactaa ctcgcagcct tactatgttt tattagatca tcagggaaaa
     2161 attcttgctg aaccacgtgg ttatacacct gacataaaaa cctacactaa atatcttgat
     2221 gaaggtttat gccgtttcga aaatcggaaa aaataaataa gattgttgct tatctaaagt
     2281 tatgttctgc taaaaacaac ttaactcttt tgtgcttcgt cagcgtactt gcttggacac
     2341 ttcaacaaaa tatcagaagc aataaactct tcgcctttca ttttaccaat aattacaatc
     2401 tgctctgact tatcaaagtc ttgcggcttg gctttgttga gcaacacttt tctttcattg
     2461 ccattgttat cataaagata aaaagtaaaa agattggcat tcttttcagg atggtattca
     2521 taaggcttat cgcggttaag tttaccaacc acatgaaata ccttaccttc atgtgatgag
     2581 gcctcagtga aattggcata ggtgccggag tcgctcactg tgctgataat agcagcaaat
     2641 gcaactgcaa tgagcaccat tgctatgata taagattttt tcatgtctgt tttttaattt
     2701 ttattcttca atttatcttc tgtctttttc aatttcctgt caatactgat taagtaaata
     2761 aacaatactg ttaacaccac agcaagcact gcaactacca cataaatctt tccattgctg
     2821 cggaaagttt cgtcaagtgc actttctgtt tgtgcaaaca catttgttgc aaccaacatt
     2881 aaaacagaaa aaagtatttt tagttttcgt gagaattcca taatttttta attcttattc
     2941 gaatagatgt tatccatacg ccaagcaatg tccatccgat aattgcagga taaaaaacca
     3001 gtcgcatctg attgtcaaga tcatattgtc caaaagcagg gttgccacca ttgccgggat
     3061 gaagtgaatc tgtcatgcgt ggcaggatgt atatgaaagc catcatcagt gggaatgcaa
     3121 aaactaaata tactgcactg agtttggcac gtttggtttc gtcttctgtt gattgtctga
     3181 gaataaaata agctgcatac atgagcatgg cagcagcagc accattgagt ttggtatcgt
     3241 taacccaaaa agcaccccaa gtgaaacgtg cccaaatcat tccggtaatt aaacctgcaa
     3301 taccaaacac cattcctgta atggcatact gttcggcttt tagatcatac tgaatctttc
     3361 ctcctgataa atagcgtatg ctgtaaacaa ttgttactac catgtgaata atcatggcaa
     3421 accacatagt tacatggaaa tataaatttc tgatagactc attaaggata ggcaactcag
     3481 gtacaggcat aaggaagcct gcaaaaagtg taaaatagag caatgctaca caagcaattt
     3541 tccagacatt atgcatgatg aaaaaagaac tttaatgtca acatgaagat aattattcgc
     3601 gccaaaggta gggaaataaa atgtaggcta aagaaggtac gagtaaattc aaagctgtta
     3661 gcatggttag gtattttaaa ttcactgaaa aatcaatacc gtcaatagca tttttagaaa
     3721 gatgaattaa aacaatcagc aaaggcaata aaacaggaaa acctaaaatt gccatgaggc
     3781 ttgactgatg ccctgcacgg gatgcaatgg cggaaatcat agtcagcaca gatgaaattc
     3841 caaagcaccc taataaaaca gtaaggataa acatgcctat gtcctgcaca ggattaccca
     3901 gtaaaattcc atagataaaa aaagtgatga tgccgaaaag aaacaacatc aaaccattat
     3961 aaattatctt tcctaaaatt acggcataag gattcagaaa tgaataatag aataactgaa
     4021 gaccacgcga ttctgccatg aagctttttg caagtgcatt tacagccata aacaaaataa
     4081 gcagccagaa caaagcattc caagccgaaa catctacaat tttcttaaaa ctgagataac
     4141 tgatataaac acctgataga ataaaaacca taagaccggc catagcggct cggttgcgcc
     4201 actccaattt ccattcttta taaattatta aacctatctg attaaacatg gcacaaagtt
     4261 gagaaaaaca agccttaaaa tgaagaaaac gaccataaat gtttaattga aagtcagtag
     4321 taaacaacaa aatgatgaaa aaaatttatt gaaaagataa ttcgaaataa attcaaattt
     4381 tgtttttgaa tttgctgcac gaatttgatt tttttttaga tttaccgtct caatttttat
     4441 ttgaaaatta taaaatcatt attaatgaaa aaagttttac taacattatt agccgcaaca
     4501 acaattattt cggctaatgc acaaagaaaa gccggcatca atatcaaaga gcagccgaca
     4561 atgacacaac gtcaatgcgg aaccggtgaa cttccattgg aatatgaaac atggatggcc
     4621 gctaaaatga aagagccgaa tgcaaataaa atgcaggtgg tttacaacat tcctgttgtt
     4681 gttcacgtaa ttcataatgg aaccaatgtg ggtgtaaacc aaaacatcag cgatgcacaa
     4741 attaattctc agattgcagt tttgaatgaa gattaccgca gacaaaatcc tgacaccaca
     4801 tcaactcctt ctgtgtttgg tgcggtagct gcagattgcg aaatcaactt ctgtttggca
     4861 caacgtgatc caagtggtaa tcctacaaat ggaattgatc gtattaacag aaatacacaa
     4921 ggctggactg cacctccata tacaaaaacc tatattgacg gcaccataaa accggcaacc
     4981 atttgggatc ctacacaata cttaaatatt tgggttgttc ctgattattc cgcttcaggg
     5041 aatcaattgc ttggccatgc tacatttcct ttaaacagta cattaacagg tttgacagga
     5101 agttacggta cggccaccaa tgatggtgta gtagtttggt ataaatcttt tggtcgtgtt
     5161 ggtaccttag atgctttata caataaaggc cgtacagcaa cgcatgaaat aggtcactgg
     5221 ttaggtctgc gtcatatttg gggcgatgct aattgtggaa atgactatgt atctgacacg
     5281 ccaacacaac aaactgcaaa ttatggtgga caaaatccgc cttatccatg tccttctttt
     5341 ccattagtaa catgtaataa tggtccaaat ggagatatgt ggatgaacta tatggactac
     5401 tgttatgaca aatgtctgaa tatgtttact gcaggacaaa aatcaagaat gcaaattgca
     5461 atggctaacg gaacataccg tgcaccctta gcaacatcac taggttgcag cgcaccttca
     5521 gctggaccaa cagcacaatt tactgcaaat acaacaacta ttgctcccgg tggcagtgta
     5581 aactttactg atcaaagcag caattcacca acatcgtggt catggtcatt cccaggtgga
     5641 actccatcat cctcaacagc ac
//
LOCUS       DAOOOL010000149         5605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1542_length_5605_cov_84.931532, whole genome shotgun sequence.
ACCESSION   DAOOOL010000149 DAOOOL010000000
VERSION     DAOOOL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_5605_cov_84.931532"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKU82_10300"
     CDS             <1..847
                     /locus_tag="PKU82_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOZ83303.1"
                     /translation="TYPAGVEVSIPKIGEKKHLISLSEKNARYYQMEKLAQYDKLNPE
                     HKTDRLMEKMKTDLRMMEQPRRIECFDNSNFQGTNAVAAMSVFINGKAAKKEYRHFNI
                     KTVEGPDDFASMQEVIFRRYKRVLDEKQPLPQLIIIDGGKGQLNAALTGLEKLGLNKK
                     IQTIGIAKKLEEIYYPGDSVPMYLDKKSETLKIIQQLRDEVHRFGITHHRNKRSHNFL
                     QSEILQIPGIGNATFEKLIIQFKSVKKIKEATTEQIAEVIGKQKAAIVVNYFNKEKQQ
                     ADEVN"
     gene            834..1802
                     /locus_tag="PKU82_10305"
     CDS             834..1802
                     /locus_tag="PKU82_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83304.1"
                     /translation="MKSTKIFYLLVVYVLLQLGWWSYLLVKLNTELGNEKLRYLETLP
                     QTEATMLQEKAIRKKLNEKYLMVAGEGLVFLSLLGIGIYYARRSFRKEFQIALQQKNF
                     MMSITHEFKSPIAGIRLSLETLLRRDVEKQQRDKILSRALLETDRINFLVENILTATR
                     IEAANMDFQFTDINLSETLSDLMAILQQSASSHNFKLKIENNIHITADKNAIHSLILN
                     LIENAQKYTPANSDIEVKLFQIDKDVFIQICDNGPGIIDIEKRKIFEKFYRIGNEETR
                     TTKGTGLGLYICRFIALKHKGRIAVQDNYPQGCIFEVVIPSLNNKT"
     gene            1805..3328
                     /gene="gpmI"
                     /locus_tag="PKU82_10310"
     CDS             1805..3328
                     /gene="gpmI"
                     /locus_tag="PKU82_10310"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOZ83305.1"
                     /translation="MNKKKVVLIIMDGWGEGRKDKSNAVYEANTPFIDSCYDKYPHSE
                     LLTSGLNVGLPDGQMGNSEVGHLNIGAGRIVYQELALINKAVEEKTLHSNAVILNCLD
                     YVKRNGKKLHLIGLVSNGGVHSHINHLKAICDIAKDNNVKDVFIHAFTDGRDCDPKSG
                     LGFIKDLENHLKTSSGKIASVVGRYYAMDRDKRWERIKEAYDLMVNGTGTNFGNAVSG
                     IEASYKNNVTDEFIKPICITENNKPVATIQDGDAVICFNFRTDRCREITMALTQQDFH
                     EQNMHKLNLEYVTMTVYDDKFTGVKTIFSKDNLHNTLGEVLQNANKSQIRTAETEKYP
                     HVTFFFSGGRENEFRGEKRILIPSPKVATYDLQPEMSALPLKDALVKELKKGETDFIC
                     INFANADMVGHTGVFSAAVKAVETVDACVKEIVQCGLEQGYSFLITADHGNADYMIND
                     DGSPNTAHTTNPVPLFLIQPQHEFNLKNGILADLAPTILQLMQVKQPSEMTGKSLLS"
     gene            3436..4341
                     /locus_tag="PKU82_10315"
     CDS             3436..4341
                     /locus_tag="PKU82_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83306.1"
                     /translation="MKKITLLLLLLSVNQLYGQSRTFFSLSGGILYYNGDLNDKSPVP
                     STKIFRNYGNAAIGIHLTRHVNLQLNYYHGSIEGADSLTKERDNKQRNLSFETRIDEI
                     SFQLSASLFSNRTKKLAIPYVFAGGGLFFFNPRARYYGSNPDYYGKLVSLQPLGTEGQ
                     YITEGDYPKPYKLYQAVFPIGIGTYIRINPKWRLKVEFSEHFTLTDYLDDASGKYPNM
                     EALSLTPKGELASYFADRRLNRSQDPSGRNRSNATKNDNYIHFGIGLVYNPQAQHPTR
                     YRGPGFWKKLFSGRKGWWGNAGNGL"
     gene            4420..5241
                     /locus_tag="PKU82_10320"
     CDS             4420..5241
                     /locus_tag="PKU82_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOZ83307.1"
                     /translation="MKILVPVDFTDITEKGFKYALSLPFANEIILFHAVDDIKKSKEA
                     QQQLSNITNKYSIPAHLTVQGKVKQGNIFETLGDVSKEVNADLIVMATHGIKGIQHFL
                     GSHAMKLITHSKTPFIVVQQKDYSPLKKMLVPVDFTKEVKQELPAVIQYAQLFNASVL
                     LLKQNNHDPYIQKRSDLNTGYFEDELDNAKIKYELIEQTFSLDDKYEVITKQAAAHNA
                     DIIVTTIEPDLDVADYVIGVEEQKIVANDLQLPVLCVNIRHFTIAGDQFSVTVQQ"
     gene            5387..>5605
                     /locus_tag="PKU82_10325"
     CDS             5387..>5605
                     /locus_tag="PKU82_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83308.1"
                     /translation="MSLVTCKEFNNLSHDEQTWYIWNGAIHLHVREVYGYRVNLFYFN
                     EFYIELWYSINENSIKKIKSFDNSALLAP"
BASE COUNT         1983 a         1012 c         1045 g         1565 t
ORIGIN      
        1 cacctatcct gcgggtgtag aggtgagtat tcctaaaata ggcgaaaaaa aacatctgat
       61 aagtctctcc gaaaaaaatg cgcgttacta tcagatggaa aaattagcac agtatgacaa
      121 actgaatcct gaacataaaa ctgacaggtt gatggagaaa atgaaaactg atttaaggat
      181 gatggagcaa ccacgcagga ttgaatgttt tgacaactca aattttcagg gcaccaatgc
      241 cgttgcagca atgagtgttt ttatcaatgg caaggcagca aaaaaagaat acagacactt
      301 caacattaaa accgttgaag ggccggatga ttttgcttcc atgcaggaag taatcttcag
      361 acgctataaa cgtgtcttag atgagaaaca acctttgccg caacttatta ttattgatgg
      421 tggcaaaggg caacttaatg ctgcattgac agggcttgaa aaattggggt taaacaaaaa
      481 gattcaaacc ataggtattg caaaaaagtt agaggagatt tattatcccg gagattctgt
      541 accaatgtat cttgacaaaa aaagtgagac cttaaaaatc attcagcaac tgagagatga
      601 agtgcatcgt ttcgggataa cacatcacag aaataaaaga agtcataatt ttctgcaatc
      661 agaaatatta caaatacccg gaataggaaa tgctacattc gagaaactga ttatccaatt
      721 taaaagtgta aaaaaaataa aagaagctac tacagaacaa attgcagaag taatagggaa
      781 acaaaaggct gcaatagttg taaattattt taacaaagaa aaacaacaag ccgatgaagt
      841 caactaaaat attttatctg ttagtcgttt atgtgctgct gcaattaggc tggtggtcgt
      901 acctcttagt gaaattaaac actgagttgg gcaatgaaaa acttcgttac ttagaaacac
      961 ttccgcaaac ggaagccacc atgcttcagg aaaaagccat tcgtaaaaaa cttaacgaaa
     1021 aatatctgat ggtagccggt gaaggtttgg tgtttctttc actgcttggc attggaattt
     1081 attatgcacg aaggtcgttc agaaaagaat ttcagatagc cttgcaacag aaaaatttta
     1141 tgatgagcat tacacatgaa tttaaatcac ctattgcagg tatcagacta agtttagaaa
     1201 cattactaag gcgtgatgta gaaaaacaac aacgcgataa aattctcagc cgtgccttac
     1261 ttgaaaccga cagaataaat tttttggtag aaaatattct tacggcaaca cgtattgaag
     1321 cagcaaatat ggattttcag tttactgata ttaatttatc agaaacctta tcagatctta
     1381 tggctatatt acagcaatct gcaagcagtc ataatttcaa attgaaaatt gaaaataata
     1441 ttcatattac agctgataaa aatgccattc attcactgat acttaacctt attgaaaatg
     1501 cacagaaata tacaccagca aattcagata ttgaagttaa gttatttcag attgacaaag
     1561 atgtatttat tcagatttgt gacaacggcc cgggcattat tgatattgaa aaaagaaaaa
     1621 tttttgagaa attctaccgt ataggtaacg aagaaacacg tactacaaaa ggtacaggtt
     1681 taggacttta catctgccgc tttattgctt taaaacataa aggtcggata gctgttcagg
     1741 ataattaccc gcaaggatgt atttttgaag tcgtaattcc atcattaaac aataagacat
     1801 gaatatgaac aagaaaaaag ttgtgctgat tattatggat ggctggggcg aaggccgcaa
     1861 agacaaaagc aatgcagtct atgaagcaaa tacgccattt atagattctt gttatgataa
     1921 atacccacac agcgagttac taacttcggg acttaatgtt ggtctcccag acggacagat
     1981 gggaaacagc gaagtaggac acctaaacat tggtgcagga agaatagtct atcaggaatt
     2041 agctctcatc aacaaagctg ttgaagaaaa aacattgcat agcaatgctg taattctcaa
     2101 ctgtcttgac tatgtaaaac gcaacggaaa aaaactgcac cttatcggct tggtgagtaa
     2161 tggtggtgtg cactcacaca ttaatcatct taaagctatt tgtgatattg caaaagacaa
     2221 taatgttaaa gatgtcttta tccatgcatt tacagatggt cgtgattgcg acccaaaaag
     2281 cggattgggt tttattaagg acttagagaa tcatctgaaa acatcttcag gaaaaattgc
     2341 ttctgttgtg ggacgatatt atgcaatgga cagagacaaa cgctgggaac gcattaaaga
     2401 agcatacgat ttaatggtaa atggaacagg caccaacttc ggcaatgccg ttagcggaat
     2461 tgaagcatct tacaaaaaca atgtcaccga tgaatttata aaacccattt gcattacaga
     2521 aaacaacaaa cctgttgcaa ccatacaaga tggtgatgca gtaatctgtt tcaatttccg
     2581 tactgaccgc tgccgtgaga taactatggc acttacacaa caagatttcc atgaacagaa
     2641 tatgcataag ctaaatctgg agtatgttac catgactgta tatgatgaca aattcacagg
     2701 tgttaaaacc attttttcca aagataattt acacaataca ttaggcgaag ttttgcagaa
     2761 tgcaaataaa tctcagatac gaactgccga aacagaaaaa tatccgcatg ttactttctt
     2821 tttttcagga ggacgagaga atgaattcag aggagaaaaa cgcatcttga ttccatcacc
     2881 taaggttgcc acttacgacc ttcaaccgga gatgagtgct ttaccattaa aagatgcctt
     2941 agtgaaagaa ctaaaaaaag gcgaaacaga ttttatctgc atcaactttg ccaatgcaga
     3001 tatggttggg catactggcg tattttccgc agctgtaaaa gcagtagaaa cagtagatgc
     3061 ctgtgtaaaa gaaattgtgc aatgtggtct ggagcaagga tattcatttt taattacagc
     3121 agatcatggt aatgccgatt atatgattaa cgatgatggc tcaccaaata ctgctcacac
     3181 aacaaatcct gtacctctgt ttcttatcca accacaacat gaattcaatt taaaaaacgg
     3241 cattctagcc gaccttgcac ctactattct gcaactgatg caagtgaagc aaccctctga
     3301 aatgactgga aaatcgttgc tttcatgaac aacattactt aacataacgc tgtgtaaaac
     3361 ataagtataa cagtgggcgt tttagaattg tagttttatg taagagacta cttttgcatt
     3421 caatcgggag tgagcatgaa aaaaattacc ctgttacttt tactactatc tgtaaatcaa
     3481 ctttacgggc aaagccgcac cttcttttca ctgtcaggag gtattcttta ttacaatggc
     3541 gaccttaacg ataaaagccc agttccatca acaaaaattt tcagaaacta tggtaatgct
     3601 gccataggta ttcatctgac aaggcatgta aacttacagc taaactacta tcatggaagc
     3661 attgagggtg ctgactcttt aactaaggag cgtgataaca aacaacgtaa cctcagcttt
     3721 gaaacacgca ttgatgaaat ttcttttcaa ctgagtgcaa gtttattctc taaccgcaca
     3781 aaaaaacttg ccatacccta tgtatttgcc ggtggtggtt tatttttctt taatccaaga
     3841 gcacgctatt atggcagtaa ccctgattat tatggaaaac tagtttcatt acaaccttta
     3901 ggcacagaag gacaatacat cactgaaggc gattatccta aaccatacaa actttatcag
     3961 gctgtatttc caataggcat tggcacgtat atcagaatca accctaaatg gcgtttgaaa
     4021 gtagaatttt ctgaacattt tacactgacc gattacttag atgatgccag tggtaaatat
     4081 cccaacatgg aagccttatc actaacacca aaaggtgaat tggcttctta ttttgcagac
     4141 agaagattaa acaggtctca ggatccttca ggaagaaacc gctcaaatgc aaccaagaac
     4201 gacaactata ttcattttgg tataggtttg gtttacaacc cacaggctca acatccaacc
     4261 cgctacagag gtcccggatt ctggaaaaaa cttttcagcg gacgcaaagg ctggtggggc
     4321 aatgccggca atggccttta agtaaaacaa tcatcctttc gtcatttatt gctttaactt
     4381 ttcatgttgt ggagtgttat cattctaaat aacatgtata tgaaaatttt agttccggtt
     4441 gactttaccg atattactga aaaaggattt aagtatgcat tgtccttacc ttttgcaaat
     4501 gaaataatcc tttttcacgc tgttgatgac ataaaaaagt ctaaagaagc acagcagcaa
     4561 ctcagcaaca ttacaaacaa atattccatt cctgcgcatc tgacggtaca gggaaaagta
     4621 aaacaaggaa acatttttga aacattaggg gatgtttcaa aagaggttaa tgccgattta
     4681 attgtgatgg ctacacatgg tataaaaggt attcaacatt tcttaggcag ccatgccatg
     4741 aagttgataa cacattcaaa aacacctttc attgtggtgc aacagaaaga ttacagtcca
     4801 ctgaaaaaaa tgcttgttcc ggttgatttc accaaagaag taaaacaaga actacctgca
     4861 gtaattcaat atgcacagct ttttaatgca agcgtattgc tactcaaaca aaacaaccac
     4921 gatccttaca ttcaaaaaag aagcgatctc aataccggtt attttgaaga cgaactcgat
     4981 aatgcaaaaa ttaagtacga attgattgaa cagacatttt cacttgatga taaatatgag
     5041 gtgattacca aacaagctgc tgcgcataat gccgatatta ttgtaaccac tattgagccc
     5101 gacttagatg ttgccgatta tgtaataggt gttgaagaac aaaaaattgt agctaacgat
     5161 cttcaattgc ctgttttatg tgtaaacatc aggcatttta caattgccgg tgatcagttc
     5221 agtgttactg tacagcagta atgtttaagt accattcgtt caacttcgtt ccattgacat
     5281 tagccaatta aaaaacgatt ttgaccaaaa ggtttatcca tctgcaagaa taagactgca
     5341 tattggttta acaattcaac aaaaaacaat cgttaaacta agaagtatgt cgttggtaac
     5401 ctgcaaagaa tttaataacc tgtcacatga tgaacaaacc tggtatatct ggaatggtgc
     5461 aattcatctt catgtaagag aagtttatgg ctacagagta aatctgtttt atttcaacga
     5521 attttacatt gaactttggt acagcattaa tgagaacagc attaaaaaga taaagtcgtt
     5581 tgacaattcc gcactgttgg cccct
//
LOCUS       DAOOOL010000150         5548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1558_length_5548_cov_46.290552, whole genome shotgun sequence.
ACCESSION   DAOOOL010000150 DAOOOL010000000
VERSION     DAOOOL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1558_length_5548_cov_46.290552"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="PKU82_10330"
     CDS             complement(<1..1889)
                     /locus_tag="PKU82_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ83309.1"
                     /translation="MSLAQQTNIYTDVEWRYKSALDLLRKEKFSAAREEFTKALEEPI
                     SEAARQDAMYYIGLCAAELYHPDAEFLLTDFISKYPQSINVSLAQFQLGRIYYKQRKY
                     RNAITHFEAVDVAYLKNDEVIEYYFKKGYSYFTRKDYDKAGKSFSQIINVESKYQTAA
                     QYYNAHVEYSRNNNKAALEQFMKLKNSESFGPLVPLYITQIYFDQQKYDEALDYAIPA
                     LKVESTQNKTEITRIVAESYYRKGNYAKAISYFEDYSKNTPQTTRQEKYQVGFCYYML
                     KDYTKAIDYLKQVLNPKDKLAQNAYFHLADCFLKTDDKQGALTAFESASKMSYDPQIQ
                     EEAMFNYAKLSVDLNMQSSTIRVMNDFMTKYPKSEHKDEITELLATLYMQTRNYKEAL
                     AALDKIRNRTATGNAAYQKVAYFRAVELFNDRDYEQSIRLLNKAITTNIDPLIQAQAI
                     YWKSEALYNQGKYDDAIKEYRIFIFTPAALKLSFYNRSNYDMGYCYYKKGNYKEAATW
                     FRKYIASRGETDNNRYNDALVRIGDAYFMQRDFANAENFYSQAVGSKASSSDYSLFQR
                     GIILGINGNNRAKADAMQQLITTYPKSNYADDAYFERAKALMVLDQDDEAELAYNKLI
                     SSYPT"
     gene            2176..3027
                     /locus_tag="PKU82_10335"
     CDS             2176..3027
                     /locus_tag="PKU82_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83310.1"
                     /translation="MNKSVIYGIVALAIVMSISSCKKDKSDADYDYSMATDMSLAENT
                     MEEVTKMADQAGDYGQLMTYRNSIPATVTVIGCATVTIENVTASIKKMTLDFGVTDVT
                     CIDGKRRRGKIFVQFEGNYRDANHEHTISFENYFVNDYKIEGTRKITNTGKNNQNQSV
                     FSIEADISISNPNGKTMTWQSQRTRTWITGENTTFNTDSINGVKDDIYSVTGSGSGKS
                     FNGTSFTANITEPLTVSLQCRFITKGKVELTPGEQAIRYIDYGDGTCDAIAAITINGV
                     TYYREIP"
     gene            complement(3087..4313)
                     /locus_tag="PKU82_10340"
     CDS             complement(3087..4313)
                     /locus_tag="PKU82_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOZ83311.1"
                     /translation="MTTVSKSGGMFSSESVLLPRESMLEVQKTKREVLIGIPKEISFQ
                     EHRIALTPDSVAVLTSNGHQVMIETGAGDSANFTDTDYSEAGARIVYSAREVYTADII
                     IKIAAPTNDELDLMHDKQTLFSVLQVNMQSKDFFLKMAKKKITAIGYEYMKDDSSLMP
                     VIQAMSEIVGRTSILIASELLSNTTGGKGELLGGIAGVPPSEVVILGAGTVGENAART
                     AMGLGADVKIFDDNMYKLRRVIRNLGEHVFTSTLTQSTMLKALRTCDVAIGALHARTG
                     RTPIVVTEEMVSEMKFGSVIIDVSIDQGGCFETSKPTNHTQPTFREYGVVHYCVPNIA
                     SRVAKTASHALSNIFTPILLNVGEHGGLESLLWKDASFRSGVYLFRGTVTNKYVSDLY
                     RLPFRDLNLLMASFTF"
     gene            complement(4310..4729)
                     /gene="tsaE"
                     /locus_tag="PKU82_10345"
     CDS             complement(4310..4729)
                     /gene="tsaE"
                     /locus_tag="PKU82_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOZ83312.1"
                     /translation="MNEHVFVCNSQDELSNVAKQILAVVGDSKIVLFKGEMGAGKTTL
                     IRAICQQLKVTDNVSSPTFSIVNEYHASDEVIYHFDFYRINNLREVYDMGYEEYFFSG
                     HRCFIEWPEKIEGIINFDYTLVQVEAKDEIRTIIVTI"
     assembly_gap    4876..4885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4886..>5548)
                     /locus_tag="PKU82_10350"
     CDS             complement(<4886..>5548)
                     /locus_tag="PKU82_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOZ83313.1"
                     /translation="VISELFRVTEEDSYFSILPTATQYARNSLFAGLMPADIEKMHPK
                     WWVNDDDEGGKNLHEEDLLKDQISRLRKNIKSSYTKITNLDAGKELVDNIPNLFGNKL
                     NVIVYNFVDMLSHARTDMQVIRELADDEPAYRSITLSWIEHSPLLEALKRIAQKNVKL
                     IITTDHGTIRVKEPSKIIGDRNTNTNLRYKQGKNLNYEKRDVFEIKNPADALLPKLNV
                     SS"
BASE COUNT         1679 a         1112 c          962 g         1785 t
ORIGIN      
        1 cttgtaggat atgaactgat gagtttattg tatgctaact ccgcttcatc atcctgatcg
       61 agtaccatca atgctttggc tctttcaaaa taagcatcat ctgcataatt tgattttgga
      121 taagtagtaa tcaattgctg catagcatct gcttttgctc tgttgttacc attaatgcca
      181 agaatgattc ctcgctgaaa caatgaataa tcagaagaag aagcctttga tcctactgcc
      241 tgcgaataaa aattttctgc attagcaaaa tcgcgttgca taaaataagc atcgccaatg
      301 cgcaccaatg catcattata acgattatta tctgtctctc ctcttgatgc tatgtactta
      361 cgaaaccatg ttgctgcttc tttgtaattt cctttcttgt aataacaata gcccatgtcg
      421 taatttgagc gattgtagaa actaagcttc aatgcagcag gtgtgaaaat aaaaatgcga
      481 tactccttta tggcatcatc atacttgccc tgattataca atgcttccga tttccaataa
      541 atagcttgtg cctgaatgag tggatctatg tttgttgtaa tagctttatt gagtaatctg
      601 attgactgtt catagtcgcg gtcgttaaat aattcaacag ctcggaaata tgcgaccttc
      661 tggtaagctg catttcctgt tgcagttctg tttctaattt tatccaatgc cgccaatgct
      721 tctttatagt ttcttgtctg catgtagagt gttgcaagta attctgttat ttcatcctta
      781 tgttcagact ttggatattt ggtcataaaa tcattcatca cacggatggt tgatgattgc
      841 atgttaaggt caacagaaag ttttgcataa ttaaacattg cttcttcctg tatctgcgga
      901 tcataactca tcttcgatgc agactcaaaa gctgttaatg caccttgctt atcgtcagtc
      961 tttaaaaaac aatctgcaag atgaaaatag gcattctgtg cgagtttgtc tttggggttg
     1021 agcacctgct tgagataatc aattgctttt gtataatctt taagcatgta gtagcaaaaa
     1081 cctacctgat acttttcctg ccgtgttgtt tgaggggtat ttttggagta atcttcaaaa
     1141 taacttatag cctttgcgta gttgccctta cgataataag actctgcaac tattctggta
     1201 atctctgtct tgttttgtgt actctctact ttcagtgccg gtattgcata atcaagtgct
     1261 tcatcatact tctgctgatc aaaataaatt tgtgtgatat acaatggcac taaaggaccg
     1321 aatgattctg aatttttcaa cttcatgaat tgctccaatg ctgccttgtt attatttctt
     1381 gaatactcta catgcgcatt gtagtattgt gctgctgtct gatattttga ttctacattg
     1441 atgatctgcg aaaaactttt tcctgcctta tcatagtctt tgcgtgtgaa atagctatac
     1501 cctttcttaa aataatactc aatcacttcg tcatttttca ggtaggcaac atctactgct
     1561 tcaaaatgag taatagcatt gcgatatttc cgctgtttgt aataaatacg tccaagttga
     1621 aattgtgcca atgaaacatt aatgctctgc ggatacttac tgatgaaatc tgtcagcaaa
     1681 aattctgcat cagggtgata taattctgct gcacataaac caatataata cattgcatcc
     1741 tgacgtgccg cttcagaaat tggctcttcc aatgcttttg taaactcttc gcgtgctgca
     1801 ctgaattttt cttttcgtaa caaatccagt gctgatttat aacgccactc cacatcggtg
     1861 taaatgttgg tttgctgtgc caacgacatc agtggaaaaa acaataacat taaatatata
     1921 cttcgcctga ccattctaac catgtttaat tgctgattta agataaaatg caaaattatt
     1981 gatgtgttta gggcgctaat gattttacgc ctgtttattt gtctttttat gaacagaaaa
     2041 atgaggataa ctaagttgtt ttcagtcata aaatttctga ttgctgatga ataatcaccc
     2101 caaaatcatg ataacaaaaa atcattgaaa ccatttaccg gaattaacgt ataggcattt
     2161 aaaaaattat ccaaaatgaa caaatcagtt atttacggaa tagttgcact tgcaatcgta
     2221 atgagtattt catcttgcaa aaaagataaa agcgatgctg actatgatta ctctatggca
     2281 acagatatgt ctttagcaga aaatacaatg gaggaagtca ctaaaatggc tgatcaggcc
     2341 ggagattacg gtcagttgat gacatacaga aatagcattc ctgcaacagt tacagttata
     2401 ggttgcgcaa cagtgaccat tgaaaatgta actgcatcta tcaaaaaaat gactcttgac
     2461 tttggagtaa ctgatgttac gtgcatagat ggcaaaagaa gacgcggaaa aattttcgtt
     2521 cagtttgaag gaaattatcg agatgccaat catgagcaca ccataagctt cgaaaattat
     2581 tttgtaaacg actataaaat tgaaggcaca agaaaaataa ccaataccgg aaaaaacaat
     2641 cagaatcaat ctgtattttc tatagaagca gatatcagta tcagtaatcc taatggcaaa
     2701 acaatgacat ggcaaagtca gcgtacacgc acatggatta ccggagaaaa cacaactttc
     2761 aataccgaca gtatcaatgg tgttaaggat gatatctact cagttaccgg aagtggcagc
     2821 ggaaaatctt ttaacggtac ctctttcact gcaaatatta ctgagccttt gactgtttcc
     2881 ttgcaatgtc gttttatcac caaaggtaaa gtagaactga ctcccggtga gcaggctatc
     2941 cgatacattg attacggtga tggcacttgc gatgctattg cagcaataac tatcaacgga
     3001 gtaacatact atagagaaat accttaatga tttttttgaa acaaaatatt caacccgcca
     3061 ttgtgcgggt tttttgttgc ctgaatttaa aatgtaaaac ttgccataag caaatttaaa
     3121 tcacgaaaag gcaaacgata caaatcactg acgtatttat tggtcaccgt acccctgaac
     3181 agataaaccc ccgaacgaaa agaagcatct ttccacaata aactttcaag tccgccatgt
     3241 tcaccaacat tcaacagaat gggagtaaaa atgttgctca atgcatgaga agctgttttg
     3301 gctacacggc ttgcaatatt aggaacacaa taatgcacca caccatactc tctgaaagta
     3361 ggttgtgtgt gattggtggg ctttgatgtt tcgaaacatc caccctgatc aatactgaca
     3421 tcaataatta cagaaccaaa tttcatttcg ctaaccatct cttctgtaac tacaattggt
     3481 gtacgtcctg tgcgtgcatg taaggcacca atggcaacat cgcaagtgcg caacgccttc
     3541 agcatggtgg actgtgttaa tgtactggta aaaacatgct cgccaagatt tctgattaca
     3601 cgtctgagtt tgtacatatt gtcatcaaaa attttgacat cagcaccaag tcccatagct
     3661 gtacgtgcag cattttctcc tactgtaccg gcaccaagta taacaacttc cgaaggtggc
     3721 acccctgcaa tgcctccaag caactctcct tttccgccag tagtgttact caataattct
     3781 gatgcaataa gtattgaagt gcgacccaca atttcactca tggcttgtat taccggcatc
     3841 agcgaagaat catccttcat atactcataa ccgattgctg taattttctt tttggccatt
     3901 ttcagaaaga aatctttgct ctgcatatta acctgcaata cagaaaacaa agtctgttta
     3961 tcatgcatta aatcaagctc atcattggta ggtgctgcga ttttaattat gatatctgcc
     4021 gtataaacct ctctggcact gtaaacaatt cgggcacctg cttcgctgta atctgtatca
     4081 gtaaagtttg cggaatcacc ggcaccggtt tcaatcatta cctgatgccc attacttgtc
     4141 agcacagcca ccgaatcggg agttaatgca atacgatgct cctgaaatga aatttctttt
     4201 ggaataccaa tcagcacttc tctttttgtc ttttgcactt caagcatgct ttcgcgtgga
     4261 agcaaaaccg attccgaaga aaacattcct ccgctctttg acacagttgt catatcgtaa
     4321 caattatggt gcgaatttcg tctttcgcct ccacttgcac aagtgtatag tcaaaattta
     4381 taataccttc aatcttttct ggccattcaa taaaacaacg gtgtccacta aaaaaatatt
     4441 cttcatagcc catatcataa acttctctca ggttattgat tctgtaaaag tcgaaatgat
     4501 aaatcacttc atcggaagca tgatactcgt ttacaattga aaaagtaggg ctgctgacat
     4561 tgtcagtcac ttttaattgc tggcaaattg cccttatcaa tgttgtctta cctgccccca
     4621 tctcgccctt aaacaaaact attttactat ctccaacaac agcaagaatc tgctttgcaa
     4681 cattgcttaa ttcatcttgc gaattacaaa caaaaacatg ctcattcatt accttcattt
     4741 gttaaccaat gaaataaaag gcacaatcat ctcttcaagc gaaatacccc catgctgaaa
     4801 tgtgtttctg taatggttta cataataatt gaagttgtta ggatatgcaa aaaatttatc
     4861 ttgtttggca aataannnnn nnnnntgcta ctcacattta attttggcaa caatgcatca
     4921 gcaggatttt ttatttcaaa gacatcacgt ttttcataat tcagattttt tccttgctta
     4981 taacgcagat tggtattggt atttctgtcg ccaataattt ttgatggctc tttaactctg
     5041 atagtgccat gatcggttgt gattatgagt tttacatttt tctgtgcaat acgtttcaat
     5101 gcctccagca atggcgaatg ttcaatccag gaaagtgtga tggaacgata tgcaggctcg
     5161 tcatctgcaa gttcgcgaat aacctgcatg tctgtacgtg catgagaaag catatccaca
     5221 aaattgtaaa ctataacatt gagtttgttg ccaaacaaat tcggaatatt atcaaccaat
     5281 tctttccctg catcaaggtt agtgatttta gtataggatg attttatatt ttttcttaaa
     5341 cggctgatct gatctttcag taaatcttcc tcatgtaaat tttttccacc ttcatcgtca
     5401 tcatttaccc accattttgg atgcattttt tcgatgtcgg caggcatgag tcctgcaaac
     5461 agactgttac gtgcatactg tgttgcagtt ggcagaatac tgaaataaga atcttcttcc
     5521 gttactctga aaagttcact gatgacag
//
LOCUS       DAOOOL010000151         5531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1562_length_5531_cov_88.858656, whole genome shotgun sequence.
ACCESSION   DAOOOL010000151 DAOOOL010000000
VERSION     DAOOOL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_5531_cov_88.858656"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKU82_10355"
     CDS             complement(<1..340)
                     /locus_tag="PKU82_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83314.1"
                     /translation="MNSLFNKQDNDRIIERIAALQPGQRALWGTMSVEQMLLHCQQPF
                     KVVFEELKLKRSLVAKLFGGIVKKSVMDDKLFKENLPTAAEFKTNHLSPQFETEKKNL
                     IAYIQKFQSAG"
     gene            549..1823
                     /gene="purD"
                     /locus_tag="PKU82_10360"
     CDS             549..1823
                     /gene="purD"
                     /locus_tag="PKU82_10360"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOZ83315.1"
                     /translation="MNILLLGSGGREHAFAQKLSESALLKHLYIMPGNAGTLQLGTNI
                     SGSVNDFDHIKKTVLEKKIDMVIVGPEEPLVNGIHDFFLNDSQLKSVPVIGPESSGAR
                     LEGSKDFSKAFMLRNNIPTARYETFESSQLQQGYDFLKTLSAPYVLKADGLAAGKGVI
                     ICETETEAREQLHHLLINQPFGKAGNKVVIEEFLKGIELSVFVLTDGNNYCLLPEAKD
                     YKRIGDNDTGLNTGGMGSVSPVPFADAAFMQKVEDKIIRPTINGLKHENISYTGFLFI
                     GLMNVNSNPFVIEYNCRMGDPETEAVFPRIKTDFIELMNSVANKKLHEVQLKTDDRTS
                     FTVMMVSGGYPETYEKGKIISGLEKVKGVNVFHAGTIIHDGKVLTNGGRVLAVNALGK
                     NLAEAREKVYGQLTEITYEKAFYRKDIGKDLM"
     gene            1999..2202
                     /gene="rpsU"
                     /locus_tag="PKU82_10365"
     CDS             1999..2202
                     /gene="rpsU"
                     /locus_tag="PKU82_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOZ83316.1"
                     /translation="MIIVQIKDNEPLDKALKKFKKKFEKTGVVKQLRSRQAFEKPSVA
                     RRTEVKKAIHRTKLQRLEAEGAM"
     gene            2298..3185
                     /locus_tag="PKU82_10370"
     CDS             2298..3185
                     /locus_tag="PKU82_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOZ83317.1"
                     /translation="MPYAKFLRFLQYEKRFSQHTLTAYGTDLKQFFDFMGQTYQAQKP
                     EDITHFFIRSWIVSLLDRKIDSRTVNRKISSLKTYFKFLMREKTISQNPMLKIVSPKT
                     AKKLPVIVDRQKMDQLLDGVAFKEGFEGIRDKLIINMFYQTGMRLAELIGLKDKNVDL
                     YNLTLKVLGKRNKERIIPITPEMKEAIVVYLNERNNIIDEKIKSEYFFIKGDGNKLYP
                     KFVYKVVNVMLSQVTTNKKRSPHVLRHTFATEMLNSGAQINAIKEILGHSSLAATQVY
                     THNTIDKLKNIYKQAHPKA"
     gene            3300..3599
                     /gene="raiA"
                     /locus_tag="PKU82_10375"
     CDS             3300..3599
                     /gene="raiA"
                     /locus_tag="PKU82_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOZ83318.1"
                     /translation="MKIKVQSIHFDADKDLIRFVEEKVDKLKQFYEHIIDGEVFLRLD
                     KNVTQENKIAEIKISTPGKVLFASEQCATFEQAADKSVEALKRQITKHKEKQRGS"
     gene            4180..5427
                     /locus_tag="PKU82_10380"
     CDS             4180..5427
                     /locus_tag="PKU82_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ83319.1"
                     /translation="MTDLFDKISKNRGPLGMHAKESHGYFTFPKLEGEISNRMIFRSK
                     EKLVWSLNNYLGLANHPEVRKIDGEASVHYGLGTPMGARMMSGETDRHTLLEQQLAAF
                     EQKEDAILLNYGYQGMVSAIDCLVDRHDIIVYDSESHACIIDGVRLHMGKRFVYPHND
                     IDNLVKQLERATKLAEETGGAILVITEGVFGMSGDQGKLKEIVALKSKFNFRLFVDDA
                     HGFGTMGKTGAGTGEEQGVQDGVDIYFGTFAKSMALIGGFIASDAQIIEYFKYNMRSQ
                     IFAKSLPMPLVEGAIKRLELLKNQPQLKNNLWKIVNALQNGLKQAGFDIGKTQSPVTP
                     VYLNGTIPEATKLIYDLRENYDIFCSMVVYPVVPKGIIMLRLIPTAVHTLEDVKYTIE
                     SFGKIKSKLLNGEYKAEKIVDVN"
BASE COUNT         1915 a          953 c         1008 g         1655 t
ORIGIN      
        1 gtcctgcaga ctgaaatttc tgaatataag ctatcagatt tttcttttct gtttcaaact
       61 gcggactcag atgattggtt ttaaattctg ctgccgtagg caggttttct ttaaatagtt
      121 tatcatccat gaccgacttt tttactatac ctccaaatag tttggccact aagctgcgtt
      181 taagtttcaa ctcttcaaac accactttaa atggttgctg gcaatgtagc aacatctgtt
      241 ctaccgacat agttccccaa agagctcttt gtccaggctg aagtgctgct attcgttcta
      301 ttattctatc attatcttgt ttattaaaaa ggctattcat cagttgttgt ttgcttttat
      361 aatatagttt ttcctttttc taatcagaat tttctacggc ataaaaataa agtaaaaaaa
      421 tcagttattg gcaccttcct gttttaatat agaaacatgt ccgttcagca ctgtattcgg
      481 gctgtcaatt aacaaacaaa atgctacggc acgccatatt tatttatcat tgcagaaaga
      541 aaattagcat gaatatttta ctgcttggat caggtggacg cgaacatgca tttgcgcaga
      601 aattgagtga gagtgcattg cttaaacact tatatattat gccgggtaat gcaggcacat
      661 tacagttggg aacaaatatt tcaggcagcg tgaatgactt cgaccacatc aaaaaaactg
      721 tgcttgaaaa gaaaattgat atggtaattg tgggccccga agagcctttg gttaatggca
      781 tccatgattt ttttcttaat gacagtcagt taaaatctgt tcctgttatt ggtccggaaa
      841 gttccggtgc cagacttgaa ggcagtaaag atttttctaa agcttttatg ttgcggaata
      901 atattcctac agcacgttat gaaacttttg aatcatcgca actgcagcag ggttatgatt
      961 ttctgaaaac actatctgca ccctacgtac taaaggctga tggtttggct gccggcaaag
     1021 gtgttattat ttgcgaaaca gaaactgagg caagagagca attgcatcat cttctcatca
     1081 atcaaccttt tggaaaggca ggaaacaaag tagtgataga agaatttctg aaaggtattg
     1141 aactctctgt ttttgttttg actgatggca acaattattg tctgctgccg gaagcaaaag
     1201 attataaacg cattggcgac aatgacaccg gactcaatac aggtggaatg ggatcagtat
     1261 caccggttcc gtttgcagat gctgctttta tgcaaaaagt agaagataaa attatcagac
     1321 caaccataaa tggattaaag catgaaaaca tttcatacac cggattttta tttatcggac
     1381 tgatgaatgt taatagcaat ccctttgtta ttgaatacaa ctgccgcatg ggcgacccgg
     1441 aaacagaagc cgtttttccg agaataaaaa ctgattttat tgagttgatg aatagtgtag
     1501 caaataaaaa acttcacgag gttcagctaa aaacagatga cagaacatcg tttactgtta
     1561 tgatggtttc gggtggatac ccagaaactt atgaaaaagg aaaaattatt tcagggcttg
     1621 aaaaagttaa aggggtaaac gtgtttcatg caggcaccat catacatgat ggcaaagtac
     1681 taaccaatgg cggacgtgta cttgctgtaa atgcattggg taaaaatctg gcagaagccc
     1741 gcgaaaaagt ctatgggcaa ttgacagaaa ttacctacga aaaagccttt tacaggaagg
     1801 atattggcaa ggatttgatg taatttccac ctgctttgtc ataaaaacag aaaagaaata
     1861 tactccatca tattaataat caatagttaa ggtcaaatac aggcaggtga gtactttctt
     1921 aaaaattttc tattgtattg attattatgc caattattta cttacctttg cgctcccaaa
     1981 acattttgta tttataatat gattattgtt caaattaaag acaatgagcc tttggataag
     2041 gctttaaaga agtttaaaaa gaagtttgaa aaaacaggtg ttgtaaaaca actgcgttca
     2101 cgtcaggcat ttgaaaaacc atcagtagcc cgcagaacag aagtaaaaaa agcaatacac
     2161 cgcacaaaac ttcagcgctt agaggccgaa ggcgcaatgt aattcttaac aaaaggcttt
     2221 ttataattca tcttttctaa gccttgttat gaactgcact tattgaatat gcctttatat
     2281 ttggcataaa caataatatg ccttacgcaa agtttcttcg atttcttcaa tacgaaaaac
     2341 gtttttcgca acatacatta actgcttatg gtaccgacct taagcagttt tttgatttta
     2401 tgggccagac ctatcaggct cagaaacccg aagatattac ccactttttt attcgcagtt
     2461 ggatagtttc tttattagac cggaaaatag attcacgaac cgtaaaccgg aaaatatcat
     2521 cactgaaaac ttatttcaaa tttttgatgc gcgaaaaaac catcagccaa aatccaatgc
     2581 tgaaaattgt ttcgccaaaa actgccaaaa aactacctgt aattgttgac cgccaaaaaa
     2641 tggatcagtt actcgatggt gttgcattta aagaagggtt tgaaggtatt cgcgacaagc
     2701 ttattataaa tatgttttat cagacaggta tgcgacttgc cgaattgata ggactaaaag
     2761 ataaaaacgt tgacctctac aatcttactt taaaagtttt gggtaaaaga aataaagagc
     2821 gcatcattcc catcacaccg gaaatgaaag aagctatagt tgtatatcta aatgaacgaa
     2881 acaacatcat tgatgaaaaa atcaagtcgg aatacttttt tattaaagga gatggtaaca
     2941 aactataccc aaaattcgtt tataaggtag ttaatgtaat gctcagtcag gtgaccacca
     3001 acaaaaaaag aagcccgcat gttttgcgcc acacttttgc aacagaaatg ctcaacagcg
     3061 gtgcgcagat aaatgccatc aaagaaattt taggacatag tagccttgcc gctactcagg
     3121 tctatacaca caacacaata gataaactga aaaatatcta caaacaggca catcctaagg
     3181 cctgatttcg tatatttact ttttataatt gaataaacac atgcccatct gatatttaaa
     3241 accacaaaac tccggcacaa gggcaatgtt tgggttttgt actaaaaaca aaatagagta
     3301 tgaagattaa agtgcagtcc atccattttg atgcagacaa agacctgatt cgttttgtag
     3361 aagaaaaggt tgacaaacta aaacagtttt acgagcacat tatagacggt gaagtatttt
     3421 tacgtttaga taaaaacgtg acgcaggaaa ataaaattgc cgaaataaaa atcagcacac
     3481 cgggcaaagt actttttgct agcgaacaat gcgccacctt tgagcaggct gccgataaat
     3541 ctgtagaagc attaaagcga cagattacaa aacataaaga aaaacaaaga ggttcttaaa
     3601 aaaacaaata gcatacagcc gcttccgata acgaagcggc ttttttgttt tataaataca
     3661 ctacatctgt ttttagaact atcatgaaac acactttcag taatcattta cctaaaaaca
     3721 atcttgcatt ttccgaaaat ttaatcgtaa aatattttta ctcatcaaca caaaaactga
     3781 attggttttt tgtttgcatt aacacagatt ttgatcaaaa cttttgcaca caattaccat
     3841 tttaagaatt ttttttgtgc tcaggttgtt gacatattaa caaacacact ccatttcatt
     3901 ttgcgcatca ctataaaaaa tcaatgctta ttaaaaatat tttttttaat gattacaaca
     3961 acaactttta ttttctcctc tgaaaaattg aaacaaaaag ttttttattt tttttcttcg
     4021 attacttttt tttcaaaaaa acttttctta tattcaaaaa agcagttaac atttttctgt
     4081 tgaaaaatat taagtagcat ggttatcaaa aataaaattg tggtgatatt tcggaaacaa
     4141 ttttaaaaag caaaagcgaa aaaaattttt agcattttta tgacagattt gtttgacaag
     4201 attagtaaaa accgtggccc gttaggtatg catgccaaag aatcgcacgg ctattttact
     4261 tttccaaaac ttgaaggtga gatttcgaac agaatgattt ttcgcagtaa agaaaagtta
     4321 gtttggagtt taaataatta tcttggattg gccaatcatc cggaagttcg caaaattgat
     4381 ggcgaagcat cggtgcatta cggattagga acaccaatgg gtgcccgcat gatgagtggc
     4441 gaaaccgaca gacatacatt attagaacaa caacttgccg ctttcgaaca aaaagaagac
     4501 gctatattat taaactacgg atatcaggga atggttagtg ctattgactg cttagttgac
     4561 cgtcatgata taatagtata tgattccgaa tcgcatgcct gcatcattga tggtgttcgc
     4621 ctgcacatgg gaaaacgttt tgtttatcca cataacgaca ttgacaacct agttaagcaa
     4681 cttgagcgtg ctaccaaact tgcagaagaa accggtggtg ctatattagt aattacagaa
     4741 ggtgtttttg gcatgagtgg cgatcagggt aagttaaaag aaattgtagc cctgaaaagt
     4801 aagttcaact tcagactttt tgttgatgat gcacacggtt ttggtaccat gggaaaaaca
     4861 ggtgccggaa caggagaaga gcaaggtgtt caggatggag tagatattta ttttggaact
     4921 tttgcaaaat caatggcact cattggtggc tttattgcca gcgatgcaca gataatagaa
     4981 tattttaaat acaacatgcg ttcgcagatt tttgcaaaaa gcctgcctat gccgttagta
     5041 gaaggtgcaa ttaaaagact tgagctacta aaaaatcagc cacagctaaa aaacaatctt
     5101 tggaaaattg ttaatgccct gcagaacgga ctcaagcaag caggatttga tattggaaaa
     5161 acacaatcac cggtgacacc ggtttatctt aatggaacta ttcctgaagc tacaaaactt
     5221 atatatgact tgcgtgagaa ctatgatatt ttctgctcga tggttgtata tcctgtagta
     5281 ccaaaaggaa ttattatgct taggttaata cctacagcag ttcatacatt agaagatgta
     5341 aaatatacaa ttgaatcttt cggaaaaata aaatcgaaat tattaaacgg tgaatataag
     5401 gcagagaaaa ttgttgacgt aaactaaaaa tgtaacttat cagcaaactc tgagtataac
     5461 aatatcaata accaataaat aaataatcat catgatgaac aattcaatga ttgaaagcac
     5521 tacaggtgct g
//
LOCUS       DAOOOL010000152         5474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1588_length_5474_cov_37.680568, whole genome shotgun sequence.
ACCESSION   DAOOOL010000152 DAOOOL010000000
VERSION     DAOOOL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_5474_cov_37.680568"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="PKU82_10385"
     CDS             <1..2152
                     /locus_tag="PKU82_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ83320.1"
                     /translation="LFAADQVLFIKDKSNGKPVNEAVVVWIMPSGKSQILLPDNNGRV
                     KLPKTEEKNFSIEVRCLGYQSQKIEKENSGDTIWLSRNEVSLNEVAVTASYAPAPAMQ
                     SAYAIKIISKESFDNMAAVNVADVLSKQLNMSTGYDQAFGSTLTMMGMDMKNIKVLID
                     GVPVIGRMNGNIDLNQLNLAAVERIEVVEGPMSTAYGTDAIAGAINLITKPQSLKKTT
                     AGASAYYENIGTYNTDLNVATNFKHAGIKITAGRNFFEGWNLTDDRRVLEWKPKTQYF
                     GTALLQYTFRKVKVAYQLGLFDEKITDKGPVTITPFSAYAYDNYFRTFRNNHSASYDW
                     FLSGKNKLNGHVAFSGYDRNKETCLKDMVALEESELTGPDFSDETKFYSWNIRNAFSH
                     VDENKNFNYQGGIDINIEKGTGKRINSTASMQEYAAFASAEYKPFSKLLIRPGLRYAL
                     HSEFTIPLIPSLNLKYDITNIQTVRLSYSRGFRTPGIKELYLDFIDINHNVQGNSNIN
                     PESSDNFQIVYSLLPEIKKVKLNSEVKLYYNKVKNLITLANYNNTSNLYTYINIGHFT
                     SQGVEWSNTIEVRSFSATAGVSYTGTSSFNESLVNDLSWYPQANGSVSYNYKPFGLTT
                     SVYYKYNGKVPVYVLDSNGNSLKQTNEDYQLLDVTVSKKLWKERVEIGGGLKNIFNVT
                     DVRRFSAGEIHNGGSSSSSLLPGRLFFLKLSVNI"
     gene            2154..3182
                     /locus_tag="PKU82_10390"
     CDS             2154..3182
                     /locus_tag="PKU82_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HOZ83321.1"
                     /translation="MRNKVSLFFFILPIAAMLFASCEKDNDKAIVLPPIDTTLLTMQV
                     NIGVNYDTVVFVSLTSGVVKSSAVKDFDLAFDAAPESKNIFLNTGKYMFAWRTQCTDM
                     MNTDTTGLNWLTDSDNMLGDSTVFGNNMISNYNANPNIVVIDRGKFNHIGSDRYRKVQ
                     VVSIDSVSYTIRYSKLNNTDYHEFVVMRDNAYSQVYFSFDNGGKMVNIAPLKNQWDMM
                     FTRYIHTYWDEPLQFRYYSVNGVITNRWNNTTCAILKKDSLPNYMPFENFNHNNISAY
                     PFHNTGNIIGFEWKFFNFNDNKYYITPDMYYIVKDKDEQYYKLKFYDFYNSTGVRGYP
                     AFCYQRIL"
     gene            3202..4440
                     /locus_tag="PKU82_10395"
     CDS             3202..4440
                     /locus_tag="PKU82_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83322.1"
                     /translation="MIRISTSIKLLIAFILPISTTAQVSETVTINPGYTNQTFYSMAN
                     ETISTIDNTDWDLAFQISGFEAAILINSKNNVKLFKANKDASEWSTMTAADTTGMYYS
                     ELLNNEAHIFNGAFNTTADTSDMFDLGWGEYDLGSHAVLGDSVYFIKLNNNTVKKIWI
                     ESLINATYNFRIADLDGSNEIQRSLQKLSYPAKNFGYYSIANDQFLDREPTKDSWDIC
                     FQQYVDHFINYKVTGVLTNDSVQSVKAYPVNDVTTATDAGYGYSYDNNLIGYDWKSFN
                     GSTFVIEDSVAYFVIDKNNHKWKMVFTGFGGSANGNFYFDKYDMGPLSVLENNSIETI
                     ALYPNPTTGSSQLIINSDNATTVTVDVTDLNGRLISSFAKKVNGLSNITIPSENFAKG
                     IYIVKINNGKQLQQLKLVVQ"
     gene            complement(4489..>5474)
                     /locus_tag="PKU82_10400"
     CDS             complement(4489..>5474)
                     /locus_tag="PKU82_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOZ83323.1"
                     /translation="YNVTINVGAFTHFNDWHINNNDTLTLDYYVKSYNLEKAKKQFEQ
                     VKPMLNCFEKYFGKYPFYRDGYKLVETPYLGMEHQSCIAYGNKYKTGYMGTDFSGIGL
                     DFDYIIIHETAHEWWGNNVTTKDIADMWVHEGFGSYAEVLYVECMYGKDKALNYVNAQ
                     RKKVGNKTPVIGPYDVNTEGSGDMYPKGSLMLHTIRHYIDNDSLFFSILMNIQKTFAY
                     QTITSADVEALMTKQSGKNLKPIFDQYLRHAAIPVFEYEVVEEKPFQLRYRWKADVAA
                     FDLPVRIDCNNENKRLNVTTQWQETTLAGFDSTCLIIRDDLGYFKVTKLNK"
BASE COUNT         1780 a          998 c          987 g         1709 t
ORIGIN      
        1 gctttttgct gccgatcagg tattatttat caaagacaaa tccaatggca agccggtaaa
       61 tgaggctgtt gtggtttgga taatgccttc cggaaagtca cagattttat tacccgacaa
      121 caatggtagg gttaaattac ctaaaacaga agagaagaat ttttcaattg aagtgcgttg
      181 tcttggttat cagagtcaaa aaattgaaaa ggaaaattcc ggtgacacca tttggctatc
      241 cagaaatgag gtcagcctca atgaggttgc cgtaactgca tcttatgctc ctgctcctgc
      301 catgcagtct gcctatgcta taaaaattat cagcaaagaa tcgtttgaca atatggctgc
      361 agttaatgtt gccgatgttc tcagcaaaca actcaacatg agcacaggtt atgatcaggc
      421 tttcggcagt acattaacca tgatgggcat ggatatgaaa aatattaaag tgttgattga
      481 tggtgttcct gttattggaa gaatgaatgg aaatatagac ctgaatcagt taaaccttgc
      541 tgctgttgaa agaatagaag tggtagaagg gccaatgagt actgcctatg gtaccgatgc
      601 cattgcaggc gctataaatc tgataaccaa accgcagtcg cttaaaaaaa ctactgcagg
      661 tgcttctgca tattatgaaa atattggaac ctacaatacc gatttgaatg ttgcaactaa
      721 ctttaaacat gcaggaataa aaataactgc cggaagaaac ttttttgaag gatggaattt
      781 aacagatgac agaagggtgc ttgaatggaa acccaagaca caatattttg gtacagcatt
      841 gctgcaatat actttcagaa aagtgaaggt tgcttatcag ttgggtttat ttgatgaaaa
      901 aattactgat aaaggtccgg taaccatcac accattttct gcctatgcct atgataatta
      961 ttttagaaca tttcgcaaca atcactcagc aagctacgat tggtttcttt ccggaaaaaa
     1021 taaattgaac ggccacgttg ctttttccgg ctatgacaga aataaagaaa cttgtctcaa
     1081 agacatggtc gcgcttgaag aatcagaatt aacaggccct gattttagtg atgaaacaaa
     1141 attttacagc tggaacatca gaaatgcatt cagtcatgtt gatgaaaata aaaatttcaa
     1201 ttatcaggga ggcatagata ttaatattga aaaaggaacg ggcaagcgca tcaacagcac
     1261 agcatccatg caggaatatg cagcttttgc aagtgcagag tataaaccat tttctaaact
     1321 acttatccgt cccggattac gctatgcttt acattcagaa tttacaattc ctttaatacc
     1381 atctcttaat ctgaaatatg atataacaaa cattcagacg gtacgtcttt catacagccg
     1441 tggttttcgt actcccggaa ttaaagaact gtatctcgat tttattgaca tcaatcataa
     1501 tgtgcaagga aatagtaaca ttaaccctga gtcgtcagat aattttcaga tagtatattc
     1561 tttattgcct gaaattaaaa aagtgaaatt aaactctgag gtaaagttgt attataacaa
     1621 agttaaaaac ctgattacat tagcaaacta caacaacact tccaatctct acacctacat
     1681 caatatcggg cattttactt cgcagggagt tgaatggtcg aatactattg aggtaagaag
     1741 tttctctgct actgccggtg tttcctatac gggtacatca tctttcaacg aatcgttagt
     1801 taatgattta tcgtggtatc cacaggcaaa tggttctgtg agttataact ataaaccttt
     1861 tggactgact acaagtgttt attataagta caatggaaaa gttccggttt atgtgttaga
     1921 cagcaatggc aacagcctca aacaaaccaa cgaagattat caactgcttg atgtaacagt
     1981 ttcaaaaaaa ctttggaaag aaagagtcga aattggagga ggacttaaaa acatatttaa
     2041 tgttaccgat gtaagaaggt tttctgcagg cgaaattcat aatggtggct catcatcatc
     2101 ctctttactt cccggacgat tatttttctt aaaactttca gtcaatattt agcatgagaa
     2161 acaaagtcag cttattcttt ttcattttac cgattgcagc aatgttattt gcctcttgcg
     2221 aaaaagataa tgataaagcc atagtattgc cgcctattga tactacattg ttgacaatgc
     2281 aagtaaatat tggtgttaac tacgatacgg ttgtattcgt tagtcttaca tcgggagtag
     2341 ttaaaagcag tgctgtaaaa gattttgatt tggcatttga tgcagcacct gagtcgaaaa
     2401 atatttttct gaacaccggt aagtacatgt ttgcttggcg cacacaatgt actgatatga
     2461 tgaatactga tacaacagga cttaactggc tcacagattc tgacaacatg cttggtgaca
     2521 gcacggtgtt tggcaataac atgatttcaa attataatgc gaacccaaat attgtagtaa
     2581 ttgacagagg taaattcaat cacatcggca gcgaccgata tcgtaaggtt caggtagtga
     2641 gcatagacag tgtttcttat acaatccgat acagtaaact aaacaacacc gattatcatg
     2701 agtttgttgt aatgagagac aatgcctatt cgcaggttta tttttcgttt gataatggtg
     2761 gtaaaatggt aaacattgct ccgctaaaaa atcagtggga tatgatgttt acccgttata
     2821 tccataccta ttgggacgaa ccattgcaat tcagatatta ctctgtaaat ggtgtaatta
     2881 ccaacagatg gaacaataca acttgtgcta ttttaaagaa agattcgtta cctaattaca
     2941 tgccattcga aaattttaac cacaataata tttcagcata tccatttcat aacacaggaa
     3001 acatcattgg ttttgaatgg aagtttttca attttaacga caataaatat tatatcactc
     3061 ccgacatgta ttatatagtt aaagacaagg acgaacagta ctacaaacta aagttctatg
     3121 acttctacaa tagtacaggt gtcagaggtt atcctgcatt ttgttatcaa cgtattttat
     3181 aaattttaaa tatacaatca aatgattcgc atttctacat caattaaatt attaatagca
     3241 tttattctgc ctatttcaac aacagcacag gtcagtgaaa cagtaactat taatcccggc
     3301 tacaccaatc agacttttta cagtatggct aatgaaacaa tatcaaccat tgacaatacc
     3361 gattgggacc ttgcatttca gatttctgga tttgaagcag ccattctcat caattcaaaa
     3421 aacaatgtaa aactatttaa agccaataaa gatgcttcag aatggagcac tatgactgcc
     3481 gcagatacaa caggcatgta ttattccgaa ttgctgaata atgaagcaca tatttttaac
     3541 ggtgcattca atactactgc cgacacatcg gatatgtttg atctcggatg gggcgaatat
     3601 gatttaggct cacatgctgt attgggcgac tcggtttatt ttataaaact aaataacaac
     3661 acagttaaaa aaatctggat tgaatctctt atcaatgcaa cttataattt tcgtattgcc
     3721 gatttagatg gcagcaatga aattcaacgc tcacttcaaa agttaagcta tcctgctaaa
     3781 aatttcggtt actactcaat tgcaaacgat cagtttttgg accgcgaacc aacaaaagac
     3841 agttgggata tttgttttca gcaatatgtt gatcatttta tcaactacaa agttactggc
     3901 gtgctcacca acgacagtgt acaatctgtt aaagcctatc ctgtgaatga tgtaactact
     3961 gctaccgatg caggttatgg ttattcatac gataacaacc ttattggcta tgactggaaa
     4021 tctttcaacg ggtcaacatt cgttattgaa gattctgttg cttactttgt cattgacaaa
     4081 aacaatcata agtggaaaat ggtattcacc ggatttggtg gctctgccaa tggtaatttc
     4141 tatttcgata agtatgatat gggtcctttg tctgttttag aaaacaatag cattgaaacc
     4201 atagctctct atcctaatcc cacaacagga agctcgcaac ttattataaa cagcgacaat
     4261 gccacaacag ttactgttga tgttactgat ttgaatggcc gtttgatttc atcattcgct
     4321 aaaaaggtaa acggactaag caacataact attccttcag aaaattttgc aaaaggaatc
     4381 tatattgtaa aaattaacaa cggcaaacaa ctgcaacaac ttaaattggt tgtacaataa
     4441 ttttgagttg attttaataa tcaggctgtc tgttattggc agcctttttt atttattcag
     4501 ttttgtaact ttaaaatagc caaggtcatc tctaatgatt aagcaggtgc tgtcaaaacc
     4561 tgcaagtgtt gtttcctgcc attgagtagt tacattcaat cttttgtttt cgttattaca
     4621 atctattctt acaggcaaat caaatgcagc aacatctgcc ttccagcggt aacgtagttg
     4681 aaaaggtttt tcttccacta cttcatactc aaaaacagga attgctgcat ggcgcaggta
     4741 ttgatcaaat attggtttaa gatttttgcc tgactgttta gtcatcagtg cttcaacatc
     4801 tgcagaagtg atggtttgat aggcaaaagt tttctgaatg ttcatcagaa tagaaaaaaa
     4861 caaagagtca ttatcaatat agtgtctgat ggtgtgtaac atcaaacttc ctttcgggta
     4921 catatcgcca ctgccttctg tgtttacgtc ataaggacca atgacgggag ttttattccc
     4981 tacctttttt cgttgcgcat tgacgtagtt taaggctttg tcctttccat acatacattc
     5041 tacatagagc acctctgcat aactgccaaa accttcatgc acccacatat cagcaatatc
     5101 ttttgtagtc acattatttc cccaccactc atgcgcagtt tcgtgaataa ttatgtaatc
     5161 aaaatctaat cctatgcccg aaaaatctgt tcccatatat ccggttttgt atttgtttcc
     5221 gtaggcaatg caactctgat gctccatacc caagtaaggt gtctcaacta acttatagcc
     5281 atcgcggtaa aaaggatatt tcccaaaata tttttcaaag caattcaaca tgggtttcac
     5341 ttgctcaaat tgttttttgg ccttctctaa gttgtatgat tttacgtagt agtcaagagt
     5401 taaagtgtcg ttgttgttta tgtgccaatc attaaaatgt gtaaaggcgc ctacatttat
     5461 tgtcacattg taag
//
LOCUS       DAOOOL010000153         5448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1608_length_5448_cov_93.692935, whole genome shotgun sequence.
ACCESSION   DAOOOL010000153 DAOOOL010000000
VERSION     DAOOOL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1608_length_5448_cov_93.692935"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKU82_10405"
     CDS             <1..624
                     /locus_tag="PKU82_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOZ83324.1"
                     /translation="LARSTKIAVDALALIINKENIDSLLTLSQFESIITGKTNNWNAI
                     NNKNNLGEVAVVFDKSGSSNVRFLKEKFLHNGNFPANCYALNSNKEVMDYVINKKNAI
                     GIIGVSWISDSNDSTAMTFLNQIKVVALKNDTSTEYTDYYQPYQAYIALKQYPLTRDV
                     FLINRQSRNGLGTGFASFVAGDQGQRLVRLKGLLPATMPVRIVRTGN"
     gene            658..2244
                     /locus_tag="PKU82_10410"
     CDS             658..2244
                     /locus_tag="PKU82_10410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ83325.1"
                     /translation="MKKTVFFLSLMFAQAVHAQTLKEAIAMTDNEQYEISGDMFKSLL
                     QKEPANGTNWFYYGENYFKSDNLDSAAIAYSKGLQAEPGNPINLVGTGKIKLEKGLNS
                     EARADFDKALTMSGSKTSLVQSEVAESFIRTKNKDLSYAIKLLNNAIAVDSKNAELYI
                     LLGDAYSEQNNGTLAAENYNRALDLDKKAVKAIVQKGVLYKRSTNYEGAAAEFQSAIK
                     LNPDFAPSYRELAETYFKMRKLDEAKQSYRKYLELSKNNSMARMRYASFLFMSGDYAD
                     AQTEINQLSSIDSSNIGMLRLSSYNAFEMNDIPKAKMLMEKIMNTTIENQRTAMDYEY
                     NGKILLKEGNDSLAAENFEKSYQIDTTKTEILQDMGSMYMKLKKYSEAVNAYERRVNN
                     GKGLTSTDYFNLARAYYFNKDYTHADTMFAKVIELLPTWPNGILWRAQTNAQLDPDSK
                     EGKAKPYYEKYIEVALTDTANSDKYKTGLIESYKYLGSYYYLIDKKPDECRSYWKKVL
                     EIAPDDKQAKQVMDGLNQKK"
     gene            complement(2320..4035)
                     /locus_tag="PKU82_10415"
     CDS             complement(2320..4035)
                     /locus_tag="PKU82_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOZ83326.1"
                     /translation="MFRFLLLFLFLDNVCLSQDVTDYYLHNRTPQWHEVIEYYKKLDK
                     QFPQAKLFTEGTTDVGKPLHLFVISSDGDFNPVSLHQKKRLIWLINNGIHPGEPDGID
                     ASMIFAKNVLSKISSEEYKNVVICIIPIYNIDGALNRGCCSRVNQDGPLEYGFRGNYQ
                     NLDLNRDFVKCDTKNTESFIRIFQKWNPDLFLDTHVSNGADYPYNITLIATQKDKLTP
                     PLGKYLSEKMLPELYRNMESKSEKMIPYVNTIKDTPDSGLEGFLETPRYASGYTALFN
                     TIGFISETHMLKPYSVRVKATITFMETLRQYAARNAAEILQKRVEAINFTKATHEFPV
                     QWQLDTLSKDSIRFDGYTAVYSTSKITGLQRLSYDKSRPYSKFIPYQNNFKPQVVIKQ
                     PFAYIIPQCWSHVIERLQLNGVEMQTITADTIIEVECYKIGQYKTTSKPYEGHYLHSN
                     VTVEKQVRKVKFFKGDRLIKVNQLANRYIVETLEPQGTDAFFAWGFFDGILQQKEWFS
                     DYVFEDIAQKMIQENPALMDSLNDAIKSDSTLKNNHYMQLNMLYRLSPYFEKSYMIYP
                     VYRLM"
     gene            complement(4041..4964)
                     /locus_tag="PKU82_10420"
     CDS             complement(4041..4964)
                     /locus_tag="PKU82_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOZ83327.1"
                     /translation="MKKFTIAIHGGAGTISPSTMTPQKETAYKQALQTALTAGYKVLE
                     ARGSALDAVTQAVIELENTPLFNAGRGSVFTYEGKHEMDASVMNGKDGMAGAVAGVRN
                     VMNPILLAKAVMQHTDFVFLHGNGAEELARAQQLTFCDDQYFFDDFRYQQWQEVKGSD
                     ATKLDHSSEKKFGTVGAVAKDLYGNLAAATSTGGMTNKRYARIGDTPIIGAGTYANNN
                     TCAVSCTGHGEYFIRSVVAYDIHCLMEYKGLSLKEACQLVVNDKLVKTGGEGGLIAVD
                     SSGTPQLVFNSEGMYRGYFSSDEQPVTEIYR"
     gene            complement(4977..>5448)
                     /locus_tag="PKU82_10425"
     CDS             complement(4977..>5448)
                     /locus_tag="PKU82_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOZ83328.1"
                     /translation="ISVGIIATKTTIASGTYQEKFSRRKPQLLFHPLATPLLASMIEE
                     GFYNDNISSAVLEEYLSYEPFKSIDGLVLACTHYPLIKKEINHFFQSKVKLFDSAEVV
                     ASKLKKILEAESLISLERKKTNRFYVSDFTQSFEHSTKIFFGKQVNLELMNLWK"
BASE COUNT         1831 a         1016 c          927 g         1674 t
ORIGIN      
        1 ctggcacgct caactaaaat tgctgttgat gcccttgcat taattatcaa taaagaaaac
       61 atagattcac tgctgacatt atcacaattt gaatccataa taacaggaaa aacaaataac
      121 tggaacgcta taaataataa aaataacttg ggtgaagtag ctgtagtgtt tgacaagagt
      181 ggctcaagta atgtacgttt tctgaaagaa aaatttttac acaatggaaa ttttcctgcc
      241 aattgttatg cactcaactc aaacaaagag gttatggatt acgttattaa taaaaagaat
      301 gcaattggta ttattggtgt tagttggata agcgacagca atgattccac tgcaatgact
      361 tttctgaatc agattaaagt tgttgcactt aaaaatgata catcaacaga atatacagat
      421 tattatcaac cttatcaggc atatatagca ttgaagcaat atccgttaac cagagatgta
      481 tttttgatta accgccagtc aagaaacgga cttggcaccg gctttgcatc atttgttgca
      541 ggcgatcagg ggcagcgtct tgtaagatta aaaggactgc tgccagctac gatgccggtt
      601 agaattgtaa gaacaggaaa ttgacagaac taaattataa ttaccaattt taatagaatg
      661 aaaaaaacag ttttttttct aagcctgatg ttcgcacagg ctgtacatgc acaaacactt
      721 aaagaggcta ttgcaatgac agataatgag caatatgaaa tttccggtga catgtttaaa
      781 tcattattgc agaaagagcc tgccaatggc accaattggt tttattatgg cgaaaattat
      841 tttaaaagtg acaacttaga ttctgctgct attgcctact caaaaggatt gcaggcagaa
      901 cccggcaatc caataaattt agtagggaca ggaaaaataa aattagagaa aggtcttaac
      961 agtgaagcac gtgccgattt cgataaagca ttaacaatgt caggctctaa aacatcgttg
     1021 gtgcaaagcg aagtcgcaga aagttttatc agaacaaaaa ataaagacct tagctacgca
     1081 attaaattac taaacaatgc cattgccgtt gattcaaaaa atgctgaatt gtacatcctt
     1141 ttaggcgatg cctattccga acaaaacaat ggcacattgg ctgcagaaaa ttacaaccgt
     1201 gcattggact tagataaaaa agctgtaaaa gctattgttc aaaaaggggt attgtataaa
     1261 cgctctacaa actatgaagg tgctgcagca gaatttcaat cagccataaa actgaacccg
     1321 gattttgcac catcttatcg cgaacttgct gaaacttatt tcaaaatgcg caagctggat
     1381 gaggcaaaac aatcctacag aaaatattta gagttgagta agaataacag tatggcaaga
     1441 atgcgttatg catcctttct gtttatgagt ggtgattatg cagatgcaca aacggaaatt
     1501 aatcaactgt cttcaattga ttcatcgaat attggtatgc ttcgtctgtc ctcatataat
     1561 gcatttgaga tgaatgatat tccaaaagca aaaatgctga tggaaaaaat catgaacact
     1621 actatagaaa accaacgcac tgcaatggac tatgaataca atggaaaaat attacttaaa
     1681 gaaggtaatg actcattagc tgctgaaaat tttgaaaagt catatcagat tgacactacc
     1741 aagacagaaa ttcttcagga catgggtagt atgtatatga aattaaaaaa atacagcgaa
     1801 gctgttaatg cctacgagcg aagagtaaac aatggtaaag gcttaacttc taccgactac
     1861 tttaaccttg caagagcata ttattttaac aaagactata cacatgcaga taccatgttt
     1921 gcaaaagtta ttgaactgtt gccaacatgg cctaatggta ttttatggcg cgcacaaacc
     1981 aatgcgcaac tcgaccccga ctctaaagaa ggtaaagcaa aaccttacta tgagaaatac
     2041 attgaagtag ctctaacaga tactgccaat tcagataaat ataaaaccgg acttattgag
     2101 tcgtataaat atcttggtag ttactattat ctgatagata aaaaacctga tgagtgcaga
     2161 tcatactgga aaaaagtact tgaaattgct cctgacgata aacaggcaaa gcaggttatg
     2221 gacggattga atcagaagaa gtaagttatt tcgataataa catttggcca ccaatcgaac
     2281 agtaaatgtg aatttactgt tcgatgtcgt tttaaccaat tacatcaatc tatacacagg
     2341 ataaatcata tatgatttct caaagtacgg tgacagtctg tacagcatat tcaactgcat
     2401 gtaatgattg ttctttaaag ttgaatctga tttaattgca tcattcaaac tatccatcaa
     2461 tgcaggattc tcctgaatca tcttttgagc aatatcttca aaaacataat cgctaaacca
     2521 ttctttttgt tgtaagatac catcaaaaaa tccccatgca aaaaatgcat ctgtgccttg
     2581 aggttccaaa gtttcaacaa tataacgatt ggcaagttga ttaactttaa tcaatctatc
     2641 acctttaaaa aatttcacct tacggacttg cttttccact gtaacattcg aatgcagata
     2701 atgtccttca taaggttttg aagtagtttt atattgaccg attttataac actcaacttc
     2761 tatgatagta tctgctgtta tggtttgcat ttctacacca ttcaactgca acctttcaat
     2821 cacatggctc caacattgtg gaatgatata ggcaaaaggt tgtttaatta ctacttgcgg
     2881 tttgaaatta ttctgatatg gaataaactt tgagtaaggt ctgcttttat cataactcag
     2941 tctttgaagc cctgtaattt tacttgtact gtaaacagca gtgtagccat caaaacggat
     3001 actgtctttt gaaagtgtat caagttgcca ttgaactgga aattcatgag ttgcctttgt
     3061 aaaattaatt gcctcaacac gtttttgtaa aatttctgct gcattacggg ctgcatattg
     3121 ccgaagtgtt tccataaatg taatagttgc ctttactctg acagaatatg gcttgagcat
     3181 gtgcgtttca gaaataaaac caatagtatt aaacaatgca gtatagccac tggcatatcg
     3241 aggtgtttcc aaaaatccct ctaaacctga atcgggagtg tccttaattg tattaacata
     3301 aggaatcatt ttttcagact tactttccat gttccgatat agttccggaa gcattttttc
     3361 agaaagatat tttcctaatg gcggtgttag tttatctttc tgtgtggcta ttaaagtaat
     3421 gttgtaagga tagtcggcac cattacttac atgagtgtct aaaaacaaat caggattcca
     3481 tttttgaaaa atacgaataa atgattctgt attcttagta tcgcacttca caaaatcacg
     3541 atttaaatcg aggttctgat aattacctct gaaaccatat tccaatgggc catcctgatt
     3601 gacgcgactg caacaacctc tgttcagtgc accatcaatg ttatagatag gaattataca
     3661 aataacaaca tttttatatt cttcagaact tatcttactc agcacatttt ttgcaaaaat
     3721 cattgatgca tcaataccat caggctcacc gggatgaata ccgttattga ttaaccatat
     3781 caatctcttt ttctgatgta acgaaacggg attaaagtcg ccatcagaag aaattacaaa
     3841 caaatgtaat ggcttaccga catcagtagt tccttctgta aaaagtttgg cctgagggaa
     3901 ctgtttatcc aacttcttat aatattcaat tacttcatgc cattgtggtg tacggttgtg
     3961 taaataataa tctgtaacat cttgtgataa acaaacattg tctaaaaata aaaacaacaa
     4021 taaaaaacga aacatataca tcagcgatat atttctgtta ccggttgttc atcagaacta
     4081 aaatatccgc gatacatccc ttctgaatta aatactaatt gtggagtacc gctactatca
     4141 actgcaatca aaccaccctc ccctccggtt ttaacgagtt tatcattcac taccaactga
     4201 catgcttctt ttagagaaag gcctttatat tccatcaggc aatgaatatc ataagccaca
     4261 actgaacgaa taaaatattc accatgacct gtacaactca ccgcacaagt attgttatta
     4321 gcataagtac ctgcgccaat aattggggta tcgccaatgc gtgcatatcg tttattagtc
     4381 atacctccgg tagaagtggc agcagcaaga tttccatata aatcttttgc aactgcgccc
     4441 actgttccga atttcttttc actgctgtga tcaagttttg ttgcatcaga ccctttcact
     4501 tcctgccatt gctgatacct aaagtcatca aaaaaatatt ggtcatcaca aaatgtcaac
     4561 tgttgtgccc ttgcaagttc ttctgcaccg ttgccatgca aaaaaacaaa gtcagtatgc
     4621 tgcatcactg ctttggcaag taaaatagga ttcatgacat ttcgaactcc tgcaactgca
     4681 cctgccattc catctttacc attcattaca gatgcatcca tttcatgctt accttcatag
     4741 gtaaaaacac tacctctgcc tgcattaaac aaaggcgtgt tctccaattc aattacagct
     4801 tgtgtaacag catctaaggc gcttcctctt gcttccaaca ctttgtatcc tgctgttaat
     4861 gcagtttgca atgcttgttt gtatgccgtt tctttctgag gtgtcatggt agatgggctg
     4921 atagtgcctg caccgccatg aattgctatt gtaaattttt tcatttgctg aaaatattat
     4981 ttccaaaggt tcatcaattc aagattcacc tgcttaccaa aaaatatttt agtgctatgt
     5041 tcgaatgatt gggtgaaatc tgaaacataa aatctattgg tctttttccg ttctaaacta
     5101 attaaagatt ctgcctccaa tatttttttc aattttgaag caactacttc agcagagtca
     5161 aacaacttaa cttttgattg aaaaaaatga tttatctcct tcttgattag tggataatgt
     5221 gtacatgcaa gtaccaatcc atcaatggac ttaaatggct cgtatgagag atattcttcc
     5281 aatacagcag aagaaatatt atcattataa aatccctctt caatcattga agcaagcaat
     5341 ggtgtggcta aaggatgaaa aagtaattgt ggctttcgtc ttgaaaattt ttcctgataa
     5401 gtacctgatg caatagttgt tttggttgca atgataccta cacttatt
//
LOCUS       DAOOOL010000154         5413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1627_length_5413_cov_69.975364, whole genome shotgun sequence.
ACCESSION   DAOOOL010000154 DAOOOL010000000
VERSION     DAOOOL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_5413_cov_69.975364"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2100)
                     /locus_tag="PKU82_10430"
     CDS             complement(<1..2100)
                     /locus_tag="PKU82_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83329.1"
                     /translation="MPRIIAFCVLYFVANVTLAQDIFMSNGVVSGCSGTFYDSGGDST
                     NYSNNENFVYTICSDQPGKCVQLSFQSFAIEKDFDFLFIYNGPSTSSPLLGTFTGTNS
                     PGTITATSGCLTIRFSSDYTVNRGGWKAYVNCINCPVNGCPTCNGGAPPPNDACSGAQ
                     NLGLLPQPAACPNGIGAVNLTNTSNLCATAEIPYQSLQGCKPIGSMSNPATDVWYKFS
                     LTAPVLDITINGLITPQVGLYSGTGCGNLVPRGCAIGGGGYLHTTFGGLAAGTYYLQV
                     SGGNINDQCNFSLSLQNNFDCSGCVMQSGLTVSPPPSNGFYLSGQQVTFCLNVTDFNP
                     TSANWLHGIVPKFGPGWDTTTIIALPPASCSNNGFWSWYSNPIISSANNKPYGPGFFY
                     ETSAGNSSNTQDNNPGNNFGDNLGQNCNLNFCWKINTLPQSLCNFGTNLNVFIETYTD
                     GESGSWTSAACANDPVHDLYATLACCIPPVVAITQPVCFGTQGSAIGTAQGSGPWTFI
                     WKNSSGVIIQQNGPVNGIDLISNLNPGTYQLITEDVNGCQSTVSFAVTQPQPLTATLA
                     VSATKCGLVNGSINITASGGTAPYSYSINNGVSFQGSPQFSNLNSGNFIITIKDAHNC
                     TVSVPAAVNGSTVPVINSLTSQNVTCYNGADGEVTVTASSGIAPYVYELKKSGLTLSQ
                     SSSIIQNVPAGTYQLTVT"
     gene            complement(2222..3136)
                     /locus_tag="PKU82_10435"
     CDS             complement(2222..3136)
                     /locus_tag="PKU82_10435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOZ83330.1"
                     /translation="MIKGKWQHSDPLLKMLLFCGIVMTCFGLISVLNIPLVKIFFGKS
                     LPEVMSGFSSGDTTYINAMKFIQIFSQLGLMLVPAWLCGWLFSDNVIQFFQFKKINFI
                     QIVVIVVVTICLSPLINLLLDWNSHLQLPASLAAIEEMMRNMETEAEKLTESFLVMHT
                     NFDLIINLLMIALLPAIGEELLFRGVLQKLIQQATKNKHWAVIITGIIFSAIHMQFFG
                     FLPRFMLGIFLGYLLVWTGSIWAPILAHFINNGSAVLLSYYEQKNAINIDEEKLGIRE
                     GEYWMVFASVIITVSACYILYRSKQKSS"
     gene            3191..4177
                     /gene="dusB"
                     /locus_tag="PKU82_10440"
     CDS             3191..4177
                     /gene="dusB"
                     /locus_tag="PKU82_10440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOZ83331.1"
                     /translation="MVNIGSVKLGEFPLLLAPMEDVSDPPFRFVCKQHGADLMYTEFI
                     SSEGLIRHAAKSTHKLDIFEYERPIGIQIFGSDVESMRQAAIIAEQAHPDLIDINYGC
                     PVKQVACKGAGAALLQDIPKMVSMTKAIVDAVKLPVTVKTRLGWDDKTKNILEVAERL
                     QDIGIKALTVHARTRSQMYKGSADWGLVGELKNNPRLTIPVFGNGDVDSPQKALEMKN
                     RYGVDGIMIGRASIGYPWIFNQIKEYLAGNCAIAVPGIAERVEVCRTHLIKSVEWKGT
                     IVGILEMRRHYTNYFKGMDHFRDFRVKLVTSNSLNELLDLLEQIKIKYKEAA"
     gene            4174..4575
                     /locus_tag="PKU82_10445"
     CDS             4174..4575
                     /locus_tag="PKU82_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOZ83332.1"
                     /translation="MTVNQPIGIFDSGIGGLTVAAAIGRLLPQEQLIYFGDTAHVPYG
                     DKSVELIGSYAERITEFLLHEQKCKAIVIACNTASAAAYSLLRDRYKGSVPVINVIDP
                     MIEAVIADDDIISVGIIATKTTIASGTYQQP"
     gene            4547..>5413
                     /locus_tag="PKU82_10450"
     CDS             4547..>5413
                     /locus_tag="PKU82_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOZ83333.1"
                     /translation="MHQVLISNLSPGVYNVVVEDGNGCMIKDTAIVNTINAPNIDSVK
                     SQSLTCYRTQDGAINIFGSGGIGTLAYSINNGSTYSATNSFNTLIAKTYTVVIKDNNN
                     CRVYQLAAVKEPPPLIIRGDIIATSCGLNNGSVNLHTDNGILPLEYSTDGGLTWSTNY
                     YYGQLPTGTITFTVRDANGCTSTRPYQIAASTAPSFDNVSVQSLKCFNTNTGSITLNS
                     INGIPPITYSIDSGATYQPTNQFQNLISGNFYLSIKDNKGCRADTLVQLSQPIPLSFT
                     SAVTNTICSYPNG"
BASE COUNT         1768 a         1026 c         1052 g         1567 t
ORIGIN      
        1 tgttacagtt agctgataag ttccggcagg aacattttga atgatgcttg aactttgtga
       61 aagtgtaagc ccgctctttt ttagttcata aacataaggt gctattccac ttgaagctgt
      121 aactgttact tcaccatctg caccattata gcatgttaca ttttgtgatg taagactatt
      181 aatcaccggt actgtgctgc cgttaacagc agcaggaact gatactgtac aattatgcgc
      241 atctttaatt gtaattataa aatttcctga attcaaatta gaaaattgcg gactaccttg
      301 aaacgaaacg ccattgttaa tgctgtaact atatggtgca gttcctcctg atgctgttat
      361 atttattgag ccatttacca atccacattt tgttgctgaa acagctaagg tggcagttag
      421 tggttgaggc tgtgttactg caaagcttac cgtactttga caaccattaa catcttctgt
      481 tatgagttga taagttccgg gattcaggtt actgattaaa tcaattccat tcaccgggcc
      541 attttgctga atgattactc cggaagaatt tttccatata aatgtccacg ggcctgaacc
      601 ttgtgcagta cctattgcag aaccttgcgt accaaagcaa acaggttgcg taattgccac
      661 caccggtgga atacaacatg caagggttgc atataaatcg tgaacaggat cgttggcaca
      721 ggcagcagat gtccacgagc cactttctcc atcggtatag gtttcaataa aaacattcag
      781 gttggtgcca aagttgcata atgattgagg tagtgtattt atcttccagc aaaaattgag
      841 attacaattc tgtccaaggt tatcaccgaa attatttccg ggattattat cttgagtatt
      901 gctgctatta cctgcagaag tttcataaaa aaatcccgga ccgtaaggtt tgttgttggc
      961 tgatgagatt attggatttg agtaccatga ccagaaccca ttgttggaac atgatgccgg
     1021 tggcaatgca attattgtcg tggtgtccca tcccggacca aatttaggta caatgccatg
     1081 aagccagttt gcagaagtag gattgaagtc ggtaacattc aaacaaaatg ttacttgttg
     1141 ccctgaaaga taaaatccat tagaaggtgg aggtgaaacc gtcagtccac tttgcattac
     1201 acagccacta cagtcgaaat tattttgaag agataaagaa aaattacatt gatcattgat
     1261 gttgccacca ctgacctgaa gataataggt gcctgcagca agaccaccaa aagtggtatg
     1321 caaataacca ccaccgccaa tagcgcaacc acgtggaact aaatttccgc atcctgttcc
     1381 ggaatataat ccaacctgtg gtgttattaa accattgatg gttatatcaa gtacaggtgc
     1441 cgtcagactg aatttgtacc aaacatcagt agccggattt gacatactgc caattggttt
     1501 acaaccctga agcgactgat atggaatttc tgcagttgca caaaggttag atgtatttgt
     1561 aagattaact gcgccaatgc cgttggggca tgccgcaggt tgtggcaata atccaagatt
     1621 ctgtgcacct gaacaagcat cgtttggcgg aggagcacca ccgttacatg tgggacaacc
     1681 attaacagga caattaatac aattaacgta tgctttccat ccacctctgt taacagtgta
     1741 atcagaagaa aatctaattg ttaaacaacc tgatgttgct gttattgtac caggagagtt
     1801 ggttcctgta aatgtaccta aaagaggaga tgaagtagaa gggccattat agataaaaag
     1861 aaaatcaaaa tctttttcaa ttgcaaatga ctgaaagctt aactgtacac atttgccggg
     1921 ttgatcactg caaatggtat aaacgaaatt ttcattatta ctgtagttgg ttgagtcacc
     1981 accgctgtca tagaaagttc cgctgcaacc gctcaccacg ccattggaca taaaaatatc
     2041 ctgcgccagc gtaacgtttg caacaaaata caatacgcaa aaagcaataa ttctcggcaa
     2101 tttggaaata ccttttaaat gaagcgtaaa gctaacaata aattgttaat aacttacacc
     2161 tgtgggaact tcaacagcac tcatgtaata agtgacaata tagagtaatt actacaacaa
     2221 cttaagagga tttttgcttg cttcgatata gtatgtaaca tgctgatact gttataataa
     2281 cagatgcaaa aaccatccag tattcacctt ctctgattcc taacttttcc tcatcaatat
     2341 tgatggcatt tttttgttca taataactga gcaacacagc cgaaccattg ttaataaagt
     2401 gagccagtat gggtgcccaa atagagcctg tccaaaccaa aagatagcct aaaaatatac
     2461 ctaacataaa cctgggcaag aacccaaaaa actgcatgtg aatagcgctg aaaataattc
     2521 cagttataat tacagcccaa tgtttgtttt ttgttgcctg ttggatgagc ttttgtaaaa
     2581 cgcctcgaaa taataattct tctccaattg caggaagcaa tgcaatcatc aaaaggttaa
     2641 tgatcaaatc aaaatttgta tgcattacta aaaaagattc agtcagtttt tctgcttccg
     2701 tttccatgtt gcgcatcatc tcttcaatcg cagctaatga agcaggtaac tgaagatgac
     2761 tgttccagtc aagtaataaa ttaattaaag gcgataaaca tattgtaaca acaacgatca
     2821 ccacaatttg aataaaatta atttttttaa attgaaaaaa ctgaatcaca ttgtctgaaa
     2881 aaagccagcc acataaccag gctggaacaa gcatgagtcc caattgtgaa aatatctgaa
     2941 taaacttcat ggcattgatg taggttgtat caccactact gaagccgctc atcacctccg
     3001 gtaaactttt gccgaaaaaa atcttaacca agggtatatt tagcaccgaa atcaagccaa
     3061 aacatgtcat cacaatgccg caaaagagta acattttcaa taaaggatca ctatgttgcc
     3121 atttgccttt tatcatcttt attaattttg cacgaaagta agcatccttg caattgttgc
     3181 agataagact atggtgaata ttggttcagt aaaattaggt gagtttccgt tgttacttgc
     3241 tcctatggag gatgtgagcg acccgccttt tcgctttgta tgtaaacagc atggcgctga
     3301 tttaatgtac acagagttta tcagctccga aggacttatt cgtcatgcag caaagagcac
     3361 acataagctc gatatttttg aatatgaaag accaatcgga attcagattt ttggttctga
     3421 tgttgaatcc atgagacagg cagccatcat tgcagagcag gcacatcccg atttgattga
     3481 tattaattac ggatgcccgg taaagcaagt agcatgcaaa ggcgcaggtg cagcattgtt
     3541 gcaggatatt cctaaaatgg tgagtatgac aaaggcaatt gttgatgcag ttaaacttcc
     3601 tgttactgta aaaacaagat taggctggga tgataaaaca aaaaatattc ttgaagttgc
     3661 agaacggctt caggatatag ggataaaagc attaactgtt catgcacgaa cacgctcaca
     3721 aatgtataaa ggcagtgccg actggggcct tgttggagag ttgaagaata atccacgact
     3781 gacaattcct gttttcggaa atggtgatgt tgactcacca cagaaagcat tggaaatgaa
     3841 aaaccgatat ggagttgatg gcataatgat tggcagagcc agtattggtt acccttggat
     3901 atttaatcag attaaagaat atcttgccgg taactgcgcc attgccgtac ccggtattgc
     3961 agaaagagta gaagtatgcc gaacacatct gataaagtct gtggaatgga aaggtaccat
     4021 tgtcggtatt cttgaaatga gaaggcacta tacaaactat tttaaaggga tggatcattt
     4081 tcgcgatttc agagtaaaat tggtgacttc aaacagctta aacgaactgc ttgatttgct
     4141 tgagcagatt aaaataaaat acaaggaagc ggcatgacag ttaatcaacc tataggtatt
     4201 tttgattccg gcattggcgg acttactgtt gctgcagcaa taggcagact gttgccacaa
     4261 gaacaactta tttattttgg cgatactgct cacgttcctt atggggataa atcggttgag
     4321 ttaattggca gttatgcaga aagaattaca gaatttttgt tgcacgaaca aaaatgtaaa
     4381 gccatagtca ttgcctgcaa cacagcatca gccgcagcat attctttatt gcgcgatcgc
     4441 tataaaggaa gtgttccggt tatcaatgtc attgacccga tgattgaggc agttattgct
     4501 gatgatgata taataagtgt aggtatcatt gcaaccaaaa caactattgc atcaggtact
     4561 tatcagcaac cttagtccgg gagtttataa tgtagtagtt gaagatggta acggttgtat
     4621 gataaaagat acagccattg taaacactat aaatgcacct aatattgata gtgtaaaatc
     4681 acaatcccta acttgttatc gtacgcaaga tggtgcaata aatatttttg gttcaggtgg
     4741 tataggtaca ttggcttata gcatcaataa tggctcaaca tattcggcaa caaactcctt
     4801 taatacactt atagcaaaaa catatacagt agttattaaa gacaacaata attgcagagt
     4861 atatcaatta gcagcagtaa aagaacctcc accattaata atcagaggtg atataattgc
     4921 aacaagctgc ggacttaata atggttcagt caacctacat actgacaatg gcattttacc
     4981 tttagagtat tcaacagatg gtggtttgac atggtcaaca aattactatt atggacaact
     5041 tccaacagga actatcacct ttacagtacg tgatgcaaat gggtgcacat ctacaagacc
     5101 ttatcaaatt gcagcatcaa ctgcaccttc ttttgataat gtttctgttc aatctttaaa
     5161 atgtttcaac accaatacag gaagtataac tctgaattcg attaatggta tccctccaat
     5221 tacatatagt attgacagtg gtgccaccta tcaaccaaca aatcagtttc aaaatcttat
     5281 ttcaggaaat ttttatttgt caattaaaga taataaagga tgcagagccg atactttggt
     5341 acaattatca caacctattc cattgtcatt tacctctgca gtaaccaata caatatgcag
     5401 ttatccaaat ggc
//
LOCUS       DAOOOL010000155         5410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1628_length_5410_cov_48.759664, whole genome shotgun sequence.
ACCESSION   DAOOOL010000155 DAOOOL010000000
VERSION     DAOOOL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_5410_cov_48.759664"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1170
                     /locus_tag="PKU82_10455"
     CDS             181..1170
                     /locus_tag="PKU82_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOZ83334.1"
                     /translation="MKEAINRIHNNKISIAMYSTIKKIWIMSVLLTSICSIKANAQYD
                     PLFTQYMFNEMFINPAYAGSRDQISATALYRNQWVGMDGAPKTQTVSLHGPVKNKKVG
                     LGISFMNESIGVEKKVGFFTNYAYRLQTSENGRLAFGLQAGLIHMKENYLDVVTHDPN
                     DIQFSQNVSKLVPNAGFGIYYNTKRFYAGFSIPRLIENYIDSVNGGDIKNKFTHQNFH
                     YFLTGGYVFDVSPEIKLKPMTMIKASDGATIQADVTLAAYFNEIFWIGLTYRTGAAAS
                     VFAQVNLTKQLRLGYSYDYTTNQLNKYNSGTHEITLGYDIPLKRTGVLSPRFF"
     gene            1190..3346
                     /locus_tag="PKU82_10460"
     CDS             1190..3346
                     /locus_tag="PKU82_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ83335.1"
                     /translation="MIISHIKKIVFVFAALLVATASFAQQDDLVLADQLYSKYSFGKA
                     IPLYEKVLSKEYNHGTASKLADCYRKNNQYKEAEKWYEKITADTNATNNEHLQYAKVL
                     ISLDKKDKAEMHLEKYLAKNPNDKLVAEQLNALKNNTFGQQQGSWIVKRLLINSVNGD
                     MCAVPYEDGIVFASSREDGKSKTQDWTSKPYLSLWFSKGKENNFSTPVLLELADKSKY
                     NDGPVSFSSDGNEMYITRNYEENGKIVRDQNRIVRLKILSATKVNGKFDAFKEFKYNN
                     KTYSCAHPFISKDGNKLYFASDMPGGKGGMDIWVCNRVNGDWDKPVNLGAEVNTTENE
                     VFPYIDHEGNLFFSSNGRSGMGGLDIFKCEFENGKFKTAENIGSPLNSTFDDFAYVYD
                     KKNMCGYLTSNRDTKNENDDLYMFKKNCVPLDGLVYDKETNLPLANAKVVINEGGNKF
                     AEVQTDDKGKFKSCLSIGQNYDFIASKDEYKENKVSINGVNDEPQVIKIPLSKIPAFT
                     LEGHVYLEEDKSALPNHPVKLMNLKTNKETETLTDANGNYHFDLEPETNYKVMTFREN
                     CADNFADRSTIGLTKSTVLKADFGFFCQGDVIRVDNIYYDLAKWNIRPDAAKELDKLV
                     DIMKKYPKMQIELGSHTDCRASFKYNMDLSEKRAKSAVQYIVKKGVEASRMTYKGYGE
                     SKLINHCECEGTRVVPCTEEEHQQNRRTEFKVLNVK"
     gene            complement(3414..3587)
                     /locus_tag="PKU82_10465"
     CDS             complement(3414..3587)
                     /locus_tag="PKU82_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83336.1"
                     /translation="MLIFYNSDGSILKMAKIQQIGEGSLLVYGSNLSSGQYSYSLVVN
                     NTTIKTKQMVKVK"
     gene            complement(3844..4734)
                     /locus_tag="PKU82_10470"
     CDS             complement(3844..4734)
                     /locus_tag="PKU82_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83337.1"
                     /translation="MDNLGDYNSIVGFTAGMYLTTGIENTFMGLRSGECNTTGSYNTF
                     YGSRAGIGQNPSTPIVGRNNSFFGYASGQAIKDGNDNIFAGSHSGNSNSSGSYNTFIG
                     GNAGYNNEKGTYNTYIGNNAGSPVSYNLHYASAIGTNATVMNDKVMILGDNDINVGIG
                     MSGVLGGPLTKLQILSKNVPQFRISYDYGNNIHTDFQTTASGDLKINPTGHFTGFDLS
                     VSPANVVDINSSSSAISGLRLRNLSTTSNSNGKVLSVDNSGDVILVRDNSVELIAMIK
                     QLQQQVNSLQQKLEATLNQH"
     gene            complement(4780..5292)
                     /locus_tag="PKU82_10475"
     CDS             complement(4780..5292)
                     /locus_tag="PKU82_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83338.1"
                     /translation="MKNKFKYIIVLSLILCNVSLIFAQSTSATFNVIIPNRPNIAGND
                     YVGWDNTVNVPLEIRHNANQSIIFYTTGIQRATILGNGRVGIGINNPLWQLDVADEIN
                     ISQPRNYYMIGGETVLHNAGTENIFTGVNCGKDILAGSASGNMNVFNGYSAGEFASGN
                     DNVFIGHSTG"
BASE COUNT         1816 a          946 c         1017 g         1631 t
ORIGIN      
        1 acagatgggg taacttggtt tataaaaaac gagactacga caattcgtgg gacggtactt
       61 caaacgttgc cggtgtttat ctcggacagc aattaccaaa cggaacatac tatttcatac
      121 tcgacctgaa tgatggcaag aagccaatgc agggatatct gacactaaaa cgataagaat
      181 atgaaagaag caataaacag aattcacaac aacaaaatca gtatagcaat gtattcaaca
      241 ataaaaaaaa tatggattat gtcagtatta ctgacctcta tctgcagcat taaagctaat
      301 gcacagtacg atccattgtt tacacaatac atgtttaacg aaatgtttat caacccggct
      361 tatgcaggct cgcgcgatca gatatcggca actgcattgt acagaaacca atgggttggt
      421 atggatggag ctccaaaaac acagactgtt tcactacatg gtcctgtgaa aaataaaaaa
      481 gtgggcttag gaatttcttt catgaatgaa tctatcggtg tagaaaagaa agtaggtttc
      541 tttaccaact atgcatatag attacaaacg agtgagaatg gccgtttagc ctttgggttg
      601 caggccgggc ttatccacat gaaagaaaac tatcttgatg ttgttacaca cgacccgaac
      661 gatattcagt tcagtcagaa tgtttcgaag ttggtgccta atgcaggatt cggaatatat
      721 tataacacca agcggtttta tgcaggtttt tctattccaa ggttgattga aaactacatt
      781 gactcagtga atggaggaga tattaaaaat aaatttacac atcagaactt tcattacttc
      841 cttaccggtg gttatgtatt tgatgtgagt ccggaaataa aattgaagcc aatgaccatg
      901 ataaaagcat ctgatggtgc aaccattcag gctgatgtaa ctttggcagc atacttcaat
      961 gaaattttct ggattggatt aacatacaga acaggtgccg ctgcatcggt ttttgcacaa
     1021 gtcaatttaa ccaaacaatt gcgccttgga tattcttatg attacacaac aaaccaatta
     1081 aacaaataca actcgggcac acatgagata acacttggct acgacattcc gcttaaacgt
     1141 acaggtgtac ttagtccacg tttcttctaa ccaatttaaa aactgtatta tgataatatc
     1201 gcatataaag aaaatagttt ttgtttttgc agcactttta gttgcaacgg caagctttgc
     1261 acagcaggat gatttggttc ttgcagacca actttattcc aagtacagct ttggaaaagc
     1321 aataccgctg tatgagaaag ttctctctaa agaatacaat catggtacgg catcaaaact
     1381 tgccgactgc tacagaaaaa acaatcaata taaagaagca gaaaagtggt atgagaaaat
     1441 tacagcagat accaatgcaa ccaacaatga gcatctgcaa tatgctaaag tgctgataag
     1501 tcttgataaa aaagacaaag ccgaaatgca tcttgaaaaa tacctagcaa aaaacccgaa
     1561 tgataaattg gtggcagagc agctaaatgc tttaaagaac aacacctttg gtcagcagca
     1621 gggatcatgg attgttaaac ggttactgat aaactctgtt aatggtgaca tgtgtgctgt
     1681 gccttatgaa gatggaattg tctttgcatc atcgcgtgaa gatggtaaaa gcaaaacaca
     1741 ggattggaca tcaaaaccat atctgtcttt atggtttagt aagggaaaag aaaataattt
     1801 cagcactccg gtcttattag aacttgccga caaatcaaaa tataatgatg ggcccgtttc
     1861 attttcttct gacggcaacg aaatgtacat tacccgaaac tatgaagaga atggaaaaat
     1921 tgtacgtgat caaaacagaa tagtaagact aaaaatacta agtgcaacaa aggttaatgg
     1981 aaaatttgat gcatttaaag aatttaaata caacaacaag acctactcat gtgctcatcc
     2041 atttatttct aaagatggca acaaacttta ctttgccagt gatatgcccg gtggaaaagg
     2101 cggcatggat atttgggtct gcaacagagt aaatggtgac tgggacaaac ctgtaaatct
     2161 tggtgccgaa gtaaacacta cagaaaatga agtatttcct tatattgatc atgagggcaa
     2221 tctgtttttc tcttcaaatg gtcgcagtgg tatgggtgga ttagatattt ttaaatgtga
     2281 gtttgagaat ggaaaattca aaactgcaga gaatatcggc agcccactca attctacctt
     2341 tgatgacttt gcttatgttt acgataagaa aaacatgtgt ggctacttga catcaaaccg
     2401 tgacactaaa aatgagaatg acgatttgta catgtttaaa aagaactgtg tgcctttgga
     2461 tggtttggtg tatgataaag aaaccaacct gccacttgcc aatgctaaag ttgtaatcaa
     2521 tgaaggaggt aataagtttg ctgaagtaca aactgatgac aaaggaaaat tcaaatcatg
     2581 tctatcaatc ggacagaatt acgatttcat tgcatcgaag gatgaataca aagaaaataa
     2641 agtatctatc aatggcgtaa atgacgagcc gcaggtaatt aaaattccat tatcaaaaat
     2701 ccctgcattt actcttgaag gtcatgttta tcttgaagaa gacaaatcag cattacctaa
     2761 ccacccggtt aaactgatga atttaaaaac aaataaagaa actgaaacac tcactgatgc
     2821 aaatggaaat tatcattttg atttagagcc tgagactaac tacaaagtga tgacctttag
     2881 agaaaattgt gcagacaact ttgctgaccg ctctactatt ggattaacaa aatcaactgt
     2941 tctgaaagca gactttggtt tcttctgtca gggtgatgta attcgtgtgg acaatattta
     3001 ttatgacttg gctaagtgga atatcagacc tgatgctgca aaagaattgg ataagttggt
     3061 tgacattatg aaaaagtatc ctaagatgca gatagaatta ggctcacata cagactgccg
     3121 tgctagcttc aagtacaaca tggatctttc tgaaaaacgt gctaagtctg ctgtacaata
     3181 tatcgttaaa aaaggtgttg aagcgtcaag gatgacttac aaaggctatg gagaatctaa
     3241 gctgattaat cattgcgaat gtgaagggac acgtgttgtg ccttgtacag aagaagaaca
     3301 tcagcaaaac agacgtacag aatttaaagt gttgaatgtg aaataagctg attatgcaat
     3361 aaaacaaagg aggcggctgc gaatgcagct gcctcctttg tttttaaaat gccctatttt
     3421 acctttacca tttgtttggt tttaatggtt gtattattga ccactaaaga ataactatac
     3481 tgacctgatg aaaggttgct accatatacc aacagagagc cttctcctat ttgctgaatc
     3541 ttagccatct ttaaaatgct gccatcggaa ttgtaaaaaa tcaacatggc atttaatggt
     3601 tcctgttgtg gtagtatgtt ccagctaatg gtggtgttgc accataaaac attgcataat
     3661 cttttatctc cttagtttta ttatgtatgg tgagaagtcc ccatttgggg atttaggggc
     3721 actctaaata tcattgattg aaaattgcct gtatattgtt agcatcaaag ctaaccagaa
     3781 aaggaaattt aaaaatttat tgtaatgtgt atggttaaag tcaatccgat ttctggataa
     3841 ttattagtgt tgatttaatg ttgcttctaa tttttgttgt aatgaattta cttgttgttg
     3901 aagttgtttt atcatagcaa ttagttctac actgttatcc ctcaccaaaa taacatcgcc
     3961 actattgtca acactaagta ctttaccatt tgaattgctt gttgtactta aatttcttaa
     4021 acgcaatccg ctaattgctg atgaggaaga attaatatca acaacatttg caggcgaaac
     4081 agacaaatca aatcctgtaa agtgtcctgt tggattgatt ttcaaatcac cacttgctgt
     4141 tgtttgaaag tcggtgtgta tattatttcc ataatcataa gaaatacgaa actgtggtac
     4201 atttttactt aaaatttgta gtttggtaag agggccgcct agaacacctg acatgcctat
     4261 gcccacgttt atatcattat ctcccaatat cattacttta tcattcataa cggtggcatt
     4321 ggtgcctatt gctgatgcat aatgaagatt ataacttaca ggcgaccctg cattatttcc
     4381 aatataagta ttataagttc ctttttcgtt attatatcct gcatttccac caatgaaagt
     4441 attatatgaa cctgaactat tgctatttcc agaatgactt ccggcaaaaa tattatcatt
     4501 accatcttta attgcctggc cactggcata accaaaaaaa gaattgttgc ggccaacaat
     4561 tggtgttgat ggattttgac caatgcctgc tcttgaacca taaaaggtgt tataacttcc
     4621 tgttgtatta cattcaccag agcgtaatcc cataaatgta ttttcaattc ctgtagttaa
     4681 atacattcca gctgtaaaac caactattga attataatca ccaagattgt ccataccggc
     4741 agaagagcct ataaatatat tggatcggcc tttactatat catcctgtac tatgacctat
     4801 aaaaacattg tcgttgccgg aagcaaattc accggcagaa tatccattga atacattcat
     4861 gtttcccgag gcacttccag ccaaaatatc ttttccacaa tttacaccgg taaaaatatt
     4921 ttctgttccg gcattatgaa gtactgtttc gccacctatc atataatagt tgcgtggctg
     4981 agatatgtta atctcatcgg ctacatctaa ttgccacaac ggattattga ttcctattcc
     5041 tactctgccg ttgcctagta tggtggcgcg ttgtattccg gttgtataga atattattga
     5101 ttggtttgca ttatgtcgaa tctcaagggg cacatttacc gtattgtccc aaccaacata
     5161 atcgttacct gctatgttgg gccgattagg aattattaca ttaaatgttg cagaggttga
     5221 ttgagcgaat attaaactta cattacataa tatcagactc aatacaatta tatatttgaa
     5281 tttatttttc ataattttca atttaaaagt gggaaaattt atttatgtaa ataccggttg
     5341 aagtgtaaat cttaaataga aaaatgccag aactaaaaga ggaaaagata ttttggctgg
     5401 aagtgcctcg
//
LOCUS       DAOOOL010000156         5350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1653_length_5350_cov_188.740699, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000156 DAOOOL010000000
VERSION     DAOOOL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_5350_cov_188.740699"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="tgt"
                     /locus_tag="PKU82_10480"
     CDS             <1..968
                     /gene="tgt"
                     /locus_tag="PKU82_10480"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOZ83339.1"
                     /translation="IILGNTYHLNLRPGIEVLHQAGGLHKFMNWSLPILTDSGGYQVF
                     SLSETRKITEEGVKFNSHIDGSKHFISPEVSMDIQRFIGADIIMAFDECTPYPCEHRT
                     AKKSMEMTHRWLQRCISHLEKSPAKYGYEQTLFPIVQGSVFKDLRTESSKVIADAGAK
                     GNAIGGLSVGEPAEIMYEITDVVTDILPVEKPRYLMGVGTPANILESIALGIDMFDCV
                     MPTRNARNGMLFTAEGIINIRNEKWKNDFSPIDESGNCFADTDYSKAYLRHLFISKEM
                     LAAQLATLHNLSFYLWLVTEARKRINDGSFLSWKNLMVKKVSQRL"
     gene            1090..1389
                     /locus_tag="PKU82_10485"
     CDS             1090..1389
                     /locus_tag="PKU82_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOZ83340.1"
                     /translation="MKKAWEKVPDFLKNKYAICIIAFVVWLAFFDKNDFYAQYRFRQQ
                     LNTLKNDKSYYADELEKSKSDLNELISSPANLEKFAREKYLMKKDDEDIFVFVEK"
     gene            complement(1424..4042)
                     /gene="clpB"
                     /locus_tag="PKU82_10490"
     CDS             complement(1424..4042)
                     /gene="clpB"
                     /locus_tag="PKU82_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOZ83341.1"
                     /translation="MDINKLTIKSQEVLQQAQFLAKSFGQQTVETGHLLKSMLEVDEH
                     VVPFLLKKASVNINSLTQKLDNIIQSYPKVSGGQVYLSTDVNDAMQRAFAALKVNKDE
                     FVSVEHLLLALLSGKDKVAKLLKEEGCNEKDLQNAINELRKGERVTSASAEETYNSLN
                     KYARNLNELARNGKLDPVIGRDDEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIINGDVPENLKSKVIYSLDMGALIAGAKYKGEFEERLKAVVKEVISGEGEIILFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAVGATTLNEYQKYIEKDKALERRFQKV
                     MVEEPDTESAISILRGLKERYETHHKVRIKDEAIIAAVELSQRYINDRYLPDKAIDLV
                     DEASSKLRLEMDSVPEELDELNRRIMQLEIEREAIKRENDTKKVNELSEEIAQLNEQR
                     NAVKARWQSEKEVVEKIQDIKEKIENLKLEAEQAERSGDYGRVAEIRYGKVREAEETL
                     SKLMEQLNEMHSKGAMMKEEVDSEDIAEVVSRWTGIPVNRMLQSEREKLLHLEAELHK
                     RVVGQDDAIEAVADAVRRSRAGLQDMRKPIGSFIFLGSTGVGKTELAKALAAYLFNDE
                     NNMTRIDMSEYQEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVILLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTANFKNTIIIMTSNIGAHLIQENFENIDERNRDEVIA
                     KTKNNVFDLLKKTIRPEFLNRIDEVIMFNPLTRNQIEEIVRIQFNQAVDMLAKNDIKL
                     SITDRAIEWVAQLGYDPQFGARPLKRVLQKKILNELSKQILAGTINKEDVILIDLDKK
                     KNFIFENEKTHTEIKA"
     gene            complement(4386..>5350)
                     /locus_tag="PKU82_10495"
     CDS             complement(4386..>5350)
                     /locus_tag="PKU82_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOZ83342.1"
                     /translation="SLQKAEVILRQYKIEKLPVVDRSGKLIGLVTYKDIIKFKSRPNS
                     CKDKLGRLRVAAGIGVTADMLDRVASLVNAGVDAIVIDTAHGHSKGVITALKSVKKKF
                     SGLDVVVGNVGTAEGAKLLVKNGADGVKVGIGPGSICTTRVVAGVGVPQLTAVADAAG
                     AIKGSGVPIIADGGIRFTGDIPKAIAAGANSIMAGSMFAGVEESPGETIIFEGRKFKS
                     YRGMGSIEAMREGSKDRYFQDAEDDIKKLVPEGIVGRVPYKGTLAETVYQLVGGLRAG
                     MGYCGAKNIAELQKAKFIRITQSGVRESHPHGITIVKEAPNYTR"
BASE COUNT         1580 a         1111 c          869 g         1790 t
ORIGIN      
        1 agattattct tggcaacacc tatcatctga atttacgtcc cggcattgaa gttttgcatc
       61 aggcaggtgg actgcataag tttatgaatt ggtcgctacc aatattaacg gatagtggcg
      121 gataccaggt tttttcttta agcgaaacaa gaaagataac agaagaaggg gttaaattca
      181 actcacatat tgatggaagc aaacatttca tttctcctga agtgagtatg gacatacagc
      241 gttttattgg tgctgatata atcatggcat ttgatgaatg tacgccctat ccatgcgagc
      301 atagaacggc aaaaaagtca atggaaatga cacatcgctg gctacaacga tgcatatctc
      361 atcttgaaaa atctccggca aagtatggtt acgaacaaac actctttcct attgttcaag
      421 gatctgtttt taaagacctg cgcacagaaa gttcaaaagt aattgctgat gccggtgcaa
      481 aaggcaatgc aattggtggt ctatccgttg gcgagcccgc tgaaattatg tatgaaatta
      541 ctgatgtggt tacagatatt ttacctgttg aaaaaccgcg ctatctgatg ggtgtaggaa
      601 ctccagcaaa tattcttgaa agtattgctt taggtataga tatgtttgat tgtgttatgc
      661 caaccagaaa tgcacgtaac ggtatgcttt ttacggctga gggtatcatt aatatcagaa
      721 acgaaaaatg gaaaaatgat ttttcgccca tagatgagtc aggaaattgt tttgcagata
      781 cagattattc caaagcttat ttaaggcatt tatttatcag caaagaaatg cttgccgcac
      841 aattagcaac acttcacaac ctgtctttct atctttggtt agttacagaa gcaagaaaaa
      901 gaattaatga tggaagtttt ctttcatgga aaaatttaat ggtgaaaaaa gttagtcagc
      961 gactgtaata ctttattgaa aaacaaacca tttgtcagct caatttgaaa taacgctttt
     1021 aacaaattgg ttctgataac ttgtaaataa caaggttatt taagttaaat gaacaagtaa
     1081 ctttaggtca tgaaaaaagc gtgggaaaaa gtacccgatt tcttgaaaaa caaatatgca
     1141 atttgcatta tagcatttgt ggtgtggctt gctttttttg acaagaacga tttttacgca
     1201 caatatcgtt tcaggcaaca gttgaatact ttaaagaatg acaaaagtta ttatgctgat
     1261 gaacttgaaa aaagtaaatc tgatttgaac gaattgattt catctcctgc aaaccttgaa
     1321 aagtttgcca gagaaaaata tctgatgaaa aaagatgacg aagatatttt tgttttcgtt
     1381 gaaaaataaa agccggaact tttaatcccg gctattataa ttgttacgct tttatttctg
     1441 tatgtgtctt ttcgttttca aaaataaaat tcttcttttt gtcaaggtca atcagaataa
     1501 catcctcttt attgattgtt cctgcaagaa tctgtttcga tagttcgttg agaattttct
     1561 tttgcagtac acgcttcaat ggtctagcac caaactgagg gtcgtatcct aactgagcta
     1621 cccactctat tgcacgatct gtaattgata acttaatgtc gttctttgcc aacatatcaa
     1681 cagcctgatt aaactgaata cgcactatct cctctatctg atttcttgta agcggattaa
     1741 acattatcac ttcatcaata cgattaagaa attcaggtct gatggttttc ttaagcaaat
     1801 caaacacatt gttctttgtc tttgcaataa cttcatctct gtttctttca tcaatgtttt
     1861 caaaattttc ctgaatcaga tgtgcaccaa tgttagatgt cataatgatg atggtgtttt
     1921 tgaaattagc agtacggcct ttattgtctg tcagcctgcc atcgtcaaga acctgcaaca
     1981 gaatattaaa tacatcagga tgtgcttttt caatttcatc aagtaatatc actgaatagg
     2041 gtcttctgcg aacagcttca gtcaactgac caccttcatc atagccaaca tatccgggag
     2101 gcgcaccaat taatctcgaa acagtatgtt tctcctgata ctcactcata tcaatcctag
     2161 tcatgttgtt ttcgtcatta aacagataag cagctaatgc ttttgcaagt tctgtttttc
     2221 ctacacctgt tgacccaaga aatatgaatg aacctattgg ctttcgcata tcttgtaatc
     2281 ctgcacgact tcttctaaca gcatcagcaa ctgcctcaat agcatcatct tgtccaacta
     2341 cacgtttatg cagctctgct tcaagatgca agagtttctc acgctcactc tgtaacattc
     2401 tgttcaccgg aatgcctgtc cagcgtgaaa caacttcggc aatatcttca ctgtccactt
     2461 cttctttcat catggcacct ttgctatgca tttcattgag ttgttccatc aattttgaaa
     2521 gtgtttcttc tgcttcacga accttgccat aacggatttc tgctactcta ccataatcac
     2581 cggagcgctc tgcttgttct gcttctaatt taagattttc aattttctct tttatatcct
     2641 gaattttttc aaccacttct ttttcactct gccagcgtgc ctttacagca tttcgctgtt
     2701 cgttgagttg tgcaatttcc tctgataact catttacttt tttagtatcg ttctcgcgtt
     2761 tgatggcttc tctttcaatt tccagctgca tgattcttct gttcaactca tctaattctt
     2821 caggaacaga gtccatttca agtcttagtt ttgaagatgc ttcatcaaca aggtcaattg
     2881 ctttatcggg aagataacgg tcgttaatat aacgttgcga aagctcaaca gcagcaataa
     2941 ttgcttcgtc ctttattctg actttatgat gtgtttcata acgttcttta agccctcgaa
     3001 gaatagaaat tgccgattct gtatcaggct cttcaaccat cactttctga aaacgtcttt
     3061 ctagcgcttt gtctttttca atgtatttct gatactcatt taaagttgtt gcaccaacag
     3121 ccctcagttc gccacgtgcc aatgcaggtt tcagaatgtt ggcagcatcc attgcaccct
     3181 cgccaccacc ggcacctaca agtgtatgta tttcgtcaat aaacaaaata atttctcctt
     3241 cgccactgat gacttctttt actacagctt ttaatcgttc ttcaaattcg cctttgtatt
     3301 ttgctcctgc aattaaagca cccatgtcca atgagtaaat aaccttcgac tttaaatttt
     3361 caggtacatc accattgata attctatgtg ccaaaccttc tgcaatagca gttttaccta
     3421 caccgggttc tccaatgagg ataggattgt tttttgttct tcttgacaaa atctgaagca
     3481 ctcttcttat ttcatcatca cgtccaatga ctgggtcaag tttaccatta cgtgctaact
     3541 cattcaggtt acgtgcatat ttattcaacg aattgtatgt ttcttctgca cttgctgaag
     3601 tcacacgttc accttttcgc aattcgttaa ttgcattttg taagtccttt tcattacaac
     3661 cttcttcttt tagaagcttt gcaactttgt ctttgcctga aagtaatgcc agtaataaat
     3721 gttcaacaga aacaaactca tctttattta cttttaatgc agcgaatgct ctttgcatag
     3781 catcgttaac atcggttgat aaataaacct gaccaccact aacctttgga tatgattgaa
     3841 tgatattgtc aagtttctgt gtcaatgagt tgatatttac acttgctttt tttaatagaa
     3901 aaggaacaac atgttcatct acctcaagca tggattttag aagatggcct gtctctacag
     3961 tttgctgtcc aaatgattta gccagaaact gtgcttgctg caatacttcc tgcgatttga
     4021 ttgttaattt atttatatcc attttaatcc ttttgttgaa tcactatcac aaatttaacc
     4081 aatactgaaa tgcgaatata aaacagtcaa ttcgtcagaa acgaattcat acaacagtca
     4141 tttttacata aacgtaaatt aaaacaagac aaattttcct gaaatagaat tttaattctg
     4201 ttacgaaaat actctgaaaa tacagagaga aagcacgata aaaaaccaat ttaaaaattt
     4261 catgttagtt cttagtgctt tagtaaataa acttctttat tttttccctt gcactgtcaa
     4321 ttaataaaac aaagttaatt gtactgcgaa caaattagtc cacatcaaac ctgataacaa
     4381 aaaaactatc tagtgtaatt cggtgcttct tttacaattg taataccatg tggatggctt
     4441 tctctgacac cgctttgtgt gatgcgtata aatttagctt tctgtaattc tgcaatattc
     4501 tttgcaccac agtaacccat acctgccctt agtccaccca ccaactgata aaccgtttca
     4561 gcaagtgttc ctttgtatgg aactcgacct acaatccctt caggtactaa ttttttaata
     4621 tcatcttctg catcctgaaa gtaacgatct tttgaacctt cacgcattgc ttcaatggag
     4681 cccatgccac gataagactt aaattttctt ccttcgaaaa tgattgtttc tcctggtgat
     4741 tcttcaacac ctgcaaacat tgaacctgcc atgatgctgt ttgctcctgc tgcaatggct
     4801 ttaggaatat cgcctgtaaa acgaatacca ccatcagcaa taataggaac accggaacct
     4861 tttatagcac ctgccgcatc agcgaccgct gttagctgcg gcactccgac acctgctaca
     4921 acacgagttg tgcagataga tccgggacca atacccactt taacgccatc agcaccattt
     4981 ttaacaagta gtttggctcc ttcagcagtt ccaacattgc ctacaacaac atctaatcct
     5041 gaaaactttt tctttacaga tttcaatgca gtgataacac cttttgaatg tccatgggca
     5101 gtatcaataa ctatggcatc aacgcctgca tttaccaatg aggcaacacg atcaagcata
     5161 tctgctgtaa caccaatgcc tgctgcaact ctcaatcgcc ctaatttatc cttgcatgaa
     5221 ttgggtcgtg acttaaattt tatgatgtct ttataagtaa ctaatcctat taattttccc
     5281 gaacggtcaa caacaggtaa tttttctatt ttatactgac gcaaaatcac ttcagctttt
     5341 tgtaaagaaa
//
LOCUS       DAOOOL010000157         5294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1677_length_5294_cov_88.859324, whole genome shotgun sequence.
ACCESSION   DAOOOL010000157 DAOOOL010000000
VERSION     DAOOOL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1677_length_5294_cov_88.859324"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PKU82_10500"
     CDS             complement(<1..981)
                     /locus_tag="PKU82_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOZ83343.1"
                     /translation="MTISSFIAAIELNKEEKMRKFLNYYKSLFFVLLSLPSINTAQAQ
                     MQQMMQRGMNAGRLYGKVVDSKTNKPVEFAAVQISVFKKDSTGTLKENILNGQLTQGN
                     GDFSLDQLPVMGELTFKIAAMGYKPYEQKVKFEIKMPQGGGQGNNWQQALNAIDKDLG
                     NIKLEPNVVALQEVQIDGTAPVLELRPDKKVFNVEKNPIAAGGTAEDVLKQVPSVNVD
                     IDGNVTLRNAAPQIFVDGRPTTLTVDQIPADAIQEVEIITNPSAKYDASGGMAGILNI
                     VLKKNRRIGYNGMVRAGADSRGRINLGGDFNVREGKVNVFLSGNLNQRKSK"
     gene            complement(1177..1764)
                     /locus_tag="PKU82_10505"
     CDS             complement(1177..1764)
                     /locus_tag="PKU82_10505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOZ83344.1"
                     /translation="MKRCFDIIVSLIAIIILLPILLLIALWIKIDSRGSIIYKQIRVG
                     KGGRDFGIFKFRTMHPDSDIKGLLTVGGRDPRITNAGYLLRKYKLDELPQLFNVLKGD
                     MSLVGPRPEVRKYVSRYSDEQLKVLSVKPGITDFASIHYSNENEILAKSANPEQTYVQ
                     EIMPHKLALNLKYIQEASLMTDLKIIAATVMKIVS"
     gene            complement(1839..3179)
                     /locus_tag="PKU82_10510"
     CDS             complement(1839..3179)
                     /locus_tag="PKU82_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOZ83345.1"
                     /translation="MAKKQDRKVFVLDTSCILFDHNAITNFKEHNVAIPITVLEEIDR
                     FKKGNDVINFEAREFIRSLDKLSSGASLGDWIPLNGPTKGKFKVLMDESGNGVDANKV
                     FGEKKADHNIINAALALKHETDERNIVLVSKDINLRLKAKSLGLQAEDYMTGKIKDIE
                     GLYTGKSELNNVSKKLIDALYHDGFCKWKDLVKSRPVANHYYILKSKAEGSSALAFYN
                     PITDRIERIHKDHAYGIKPRNAEQAFAMHAVMNPNVLLVTVQGVAGTGKTLIALASAM
                     QQKMNYRQIYLARPIVPLSNKDIGYLPGDIKSKINPYMEPLWDNLKYIQSQYRETDKE
                     YQKISDMVENEKLHITPLAYIRGRSLSNICFIIDEAQNLTPHEVKTIITRAGEGTKII
                     FTGDVFQIDTPYLDAHSNGLSNVIDKMKNSHLYSHITLEKGERSELANLAGELL"
     gene            complement(3323..4108)
                     /gene="truA"
                     /locus_tag="PKU82_10515"
     CDS             complement(3323..4108)
                     /gene="truA"
                     /locus_tag="PKU82_10515"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOZ83346.1"
                     /translation="MQRYFLELFFRGTHFSGWQIQKNAAAVQQKINEALGLLINGSAL
                     KPIETTGCGRTDTGVHATQFFAHFDAPVIEDHRNFLYQLNGILPGDITILNLHVVDSD
                     CHARYDAVSRTYQYYIHTYKNGFLEEFSGCYYRRFDIALMQKAADILLKHDDFNSFCK
                     SRAQSKTSLCKITFAKWSESKGKLMFEITADRFLRGMVRALVGTMTDVGIGKISLNDF
                     ENIIAARNRRKAGHAAPACGLYLTKIVYPFLNVKSKNHLPHLL"
     gene            4250..5236
                     /locus_tag="PKU82_10520"
     CDS             4250..5236
                     /locus_tag="PKU82_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ83347.1"
                     /translation="MFDIREINEKIQRESEFIDLLQLELSKVIVGQKYMVERLLIGLL
                     SNGHLLLEGVPGLAKTLAIKSLASAIDARFSRIQFTPDLLPADLVGTLMYNQKKEEFA
                     VRKGPIFANFILADEINRAPAKVQSALLEAMQEKQVTIGDETFKLEEPFLVLATQNPI
                     EQEGTYPLPEAQLDRFMLKVVIGYPTREDEKLIIRQNLSQEKTNITPIIRTADILKAR
                     QLVREVYMDEKIEKYIIDIVFATRFPNDNKLGKIAQLINYGGSPRASINLALAAKTYA
                     FIKRRGYVIPEDVRAICHDVMRHRIGLTYEAEAENYTTDMVINEVLNVVEVP"
BASE COUNT         1635 a         1082 c          845 g         1732 t
ORIGIN      
        1 ttttgattta cgctgattga gattgccgct taaaaacaca tttacttttc cttcgcgcac
       61 attaaaatct ccccccagat ttattcttcc tcttgaatct gcaccggcac gaaccattcc
      121 gttatagcca atgcgtctgt tctttttcaa aacaatgttt aatataccgg ccatccctcc
      181 gctggcatcg tactttgctg atggatttgt aataatttca acttcctgaa tggcatcagc
      241 aggaatctga tctacagtta aagttgttgg acggccatct acaaatattt gcggtgcggc
      301 attccgcaag gttacattac catctatatc aacattcaca ctaggaactt gctttagcac
      361 atcttctgct gtacctcctg ctgcaattgg atttttttca acattaaaaa ctttcttgtc
      421 gggacgcagt tccaacaccg gagctgtacc atcaatctga acttcctgaa gtgcaaccac
      481 attaggttca agctttatgt tacctaaatc tttatctata gcattgagtg cctgttgcca
      541 attatttccc tgaccaccac cttgaggcat ttttatttca aattttactt tttgctcata
      601 aggtttatat cccattgcag caattttaaa agtcagttca cccattaccg gcaactgatc
      661 caaactaaaa tctccattgc cctgcgttaa ctgtccattc agaatatttt cctttaatgt
      721 gcccgtactg tcctttttaa aaacagaaat ctgaactgca gcaaattcaa caggcttatt
      781 ggttttggaa tctacaactt ttccatagag ccgaccggca ttcataccgc gttgcatcat
      841 ctgctgcatt tgtgcttgcg ctgtgtttat acttggcaaa ctcaacaaaa caaaaaacaa
      901 gcttttgtaa taattcaaaa atttacgcat cttctcttct ttatttaatt caatggcagc
      961 tataaaacta cttatagtca atttaattct ttactgttca attcaattgc aacaataaca
     1021 cttttttcct ctttaaagtt taagcggatt taaaacttat ttcatcaact gaaaactcat
     1081 tttaaaatca tttactctca ctcaaacaat agaaaaatta acacccatta acacagtttt
     1141 aaaaacaaat taaaggtcta taaataaact tattattcaa ctgacaattt tcatgactgt
     1201 agcggcaata atcttcaggt ctgtcattag gcttgcttct tgaatatact ttagattgag
     1261 tgctaactta tgtggcataa tttcctgtac ataggtctgt tcagggtttg ctgacttcgc
     1321 taaaatttca ttctcattgc tgtaatgaat gcttgcaaaa tcagtaattc ccggttttac
     1381 gcttaatacc tttagttgtt catcggaata acgtgaaaca tacttgcgca cttccggtct
     1441 tggaccaaca agactcatat cgcctttcaa cacattaaac aattgcggta attcatcaag
     1501 tttatactta cgaagtaaat accctgcatt ggttattctt ggatcacgtc cacccacagt
     1561 cagcaatccc tttatgtcag agtcagggtg catcgttctg aatttaaaaa taccaaaatc
     1621 tctacctccc ttacctactc ttatttgttt gtaaataata ctacctctgc tatcaatttt
     1681 aatccataat gcaatgagca atagtattgg caaaagaata attattgcta ttaatgatac
     1741 aataatatca aagcatcgtt taatcatgtc gcaaagataa cttctgtatc tctacctacc
     1801 ttgaataaaa attatattta aaaaatccgg ttacttgttt aaagtaattc accggcaaga
     1861 tttgccaact cacttcgttc tcctttttcc aaagttatgt gcgaatagag atgagaattt
     1921 ttcatcttat caattacatt gcttagtcca ttgctatgtg catcaagata tggtgtatca
     1981 atctgaaaca catcacctgt gaaaattatt tttgttcctt caccagcacg ggtaatgata
     2041 gttttaactt catgtggtgt cagattttga gcttcgtcaa taataaaaca aatattactt
     2101 aagctacggc cacgtatata tgccaatggt gtgatgtgca atttttcgtt ctctaccata
     2161 tctgaaattt tctgatactc tttatctgtt tcgcgatatt gactttgaat gtatttaaga
     2221 ttatcccaca atggctccat gtatggatta atctttgact ttatatctcc gggcaaataa
     2281 ccaatatctt tattgctgag cggaactatc ggacgcgcaa gatatatctg acggtaattc
     2341 attttttgtt gcattgccga tgctaatgca attaaagttt tacctgttcc ggccacaccc
     2401 tgaaccgtta ccaacaaaac attcggattc atgactgcat gcatggcaaa agcctgttcg
     2461 gcattacgag gttttatacc ataagcatga tctttatgaa tacgttcaat tctatcggtt
     2521 ataggattat aaaacgccaa tgcagaagag ccttctgcct tacttttaag aatataataa
     2581 tgattggcaa ccgggcgtga ttttaccaaa tctttccact tacaaaaacc gtcatgatac
     2641 aatgcatcaa ttaatttttt agatacattg ttcaactcag atttaccggt ataaagtcct
     2701 tcaatatctt taatttttcc ggtcatataa tcttctgcct gtaggccaag cgatttagct
     2761 ttcagtcgca gattaatatc tttacttacg agaacaatat ttcgttcatc ggtttcatgc
     2821 ttcaacgcca gtgctgcatt aataatgtta tggtcagctt ttttctcacc gaaaacttta
     2881 ttggcatcta caccatttcc gctttcatcc atcagcacct taaactttcc tttggtgggg
     2941 ccattgagtg gaatccaatc gccaagtgat gcgcccgaac ttaacttatc gagtgatctg
     3001 ataaactcac gtgcttcgaa attgattacg tcatttccct ttttaaaacg atctatttct
     3061 tcaagtaccg ttatagggat ggccacattg tgttctttaa agttagtaat tgcgttgtgg
     3121 tcgaaaagga tgcaagaggt atcgagtacg aatactttgc gatcttgttt ttttgccatg
     3181 ctaaaatttg tttggtctat gacaaatata tagccggcat cacaggttga aaacttaaac
     3241 cattgcgtat attacacaaa ggtttgttaa taacagatag ccttgccgca attcatttta
     3301 tctctgtaat agcagcagta ttttataaaa ggtgtggtaa atgatttttg cttttaacat
     3361 taagaaacgg atacacaatt tttgtaagat acaatccaca tgcaggtgct gcatgccctg
     3421 cctttcttct gttgcgtgca gcaataatat tttcaaaatc gttgagacta atctttccaa
     3481 tgccaacatc agtcattgta ccaactaatg cacgcaccat acctcttaaa aatctgtcgg
     3541 cagtaatttc aaacattaac ttccctttgc tttctgacca tttagcaaaa gtgattttac
     3601 acagacttgt cttactctga gcacgtgatt tacaaaaact attaaagtcg tcatgtttta
     3661 aaagtatatc agctgctttt tgcatgagag caatatcaaa tctacggtaa taacatcctg
     3721 aaaactcctc taaaaatcca tttttataag tgtggatata atactgatat gtacggctca
     3781 ctgcatcata tcgggcatga caatcgctat caacaacatg cagatttaaa attgtaatat
     3841 caccgggcaa aattccgttt agttgataaa gaaaatttct atggtcttca ataacaggag
     3901 catcaaaatg tgcaaaaaac tgtgtggcat gaacacctgt atctgtacga ccgcagccag
     3961 tagtttcaat tggttttaaa gcagagccat tgattagcag acccaaggct tcgttaattt
     4021 tctgctgtac agcagctgcg ttcttctgta tctgccagcc actgaaatgc gtacctctaa
     4081 aaaataactc aagaaaatat cgttgcatag cgtacaaaaa taaactatct gattatgctc
     4141 tgaatagtat atttgccaat tctattactt aacacaaatt aacaatctat ttcacattct
     4201 tttcacagct aaacttttaa acttgcatcg caaataatca tcaatattta tgtttgatat
     4261 cagagaaata aacgaaaaaa tccaacgcga aagtgaattc attgacttgc tgcagttaga
     4321 actgagcaag gttattgttg gacagaaata catggtagaa agactactca ttggcttact
     4381 ctccaacggt cacttattac ttgaaggtgt tcccggtctt gcaaaaacct tagccattaa
     4441 atcacttgcc tctgcaattg atgcacgttt cagccgcatt cagtttactc ctgatttatt
     4501 acctgccgat ttagtaggga cgctaatgta taatcagaaa aaagaagaat ttgcagttcg
     4561 taaaggaccc atttttgcaa attttattct tgcagatgaa ataaaccgtg cccctgcaaa
     4621 agtgcaaagt gccttgcttg aagcgatgca ggaaaaacaa gtcactattg gtgatgagac
     4681 ttttaaactt gaagaacctt tcttagtact tgccactcag aatcctattg aacaggaagg
     4741 aacctatccg ctacccgaag cacaattaga tcgttttatg ctaaaggttg taataggata
     4801 tccaacaaga gaagatgaga aattgattat ccgtcagaat ttatcacagg aaaaaacaaa
     4861 catcacgcct ataatccgca ctgctgatat tcttaaagca agacaattgg ttcgcgaagt
     4921 ttatatggat gagaaaatcg aaaaatatat tattgatata gtttttgcca cacgttttcc
     4981 gaatgacaac aaactcggaa aaattgcaca actcatcaac tatggaggat caccacgtgc
     5041 aagcataaac cttgcgcttg cagcaaaaac ttatgcattt ataaaacgca gaggttatgt
     5101 tattcctgaa gatgtgcgtg ctatttgtca tgatgtaatg cgtcatcgta ttggcttgac
     5161 ttatgaagca gaagcagaaa attacacaac tgatatggtg attaacgaag tacttaacgt
     5221 ggttgaagtg ccttagagat taaaaacatt caacaaattt aattcaacag aatcaataaa
     5281 caattgccaa cgct
//
LOCUS       DAOOOL010000158         5215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1717_length_5215_cov_62.917636, whole genome shotgun sequence.
ACCESSION   DAOOOL010000158 DAOOOL010000000
VERSION     DAOOOL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1717_length_5215_cov_62.917636"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1811)
                     /locus_tag="PKU82_10525"
     CDS             complement(261..1811)
                     /locus_tag="PKU82_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83348.1"
                     /translation="MKKYILFILLIAVKISTAQQGINNNWLMGYSSFGGLPFGNTKFD
                     FFNGIPIINYDSLEMDFRHTHANISDAQGNLLFYTNGYYIADASNDTMQNGSGINPGA
                     YADQFSDGFQIPQGALIIRQPGSSSIYYMFHNTLDNHPVPGAYSLYFYLTTIDMSLNG
                     GLGTVISKNQILLTDSMVTGKITACKHANGRDWWVMTHQINTNKFFRFLVTTNNLILG
                     PYSQNIGAIRPQDAGQAWFSPDGSRFAYFYLNGGLDIFDFNRCTGQLSNPVHIAISFV
                     GGYNVGLAFSPNSDVLYVSNVFYVYQFDLTAANIAASVQTVATYDGFMSVPPNCPTCP
                     SLQTLFSNAGLAPDGKVYISTGNGTVHWHTIDNPDVVGMGCNVNQHSVQVPAYYFNTV
                     PNHPNYFLECDSTLGCLCAPTVGLPTPQPPKGGVAAMPNPSDGNFTLQFDVKSMSGEL
                     EIYDVMGKLVYKDYVSAWSQFKRVSLTPRDEVGGLTPLQGLGLSGGIYNCILKRGSEI
                     ERVKVIVN"
     gene            2014..2727
                     /locus_tag="PKU82_10530"
     CDS             2014..2727
                     /locus_tag="PKU82_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOZ83349.1"
                     /translation="MIIEIWSDIMCPFCYIGKRHYENALKQFAGKNNIELKWKSYLLD
                     PETPLEFEKPLNVYEYLAERKGISLAQSIKMHEHVVAMAKQAGLDYQLDKLVIANMVK
                     AHRVIQFAKKKGLADLIEENMFKAYFTEGKNLADDDVLVAIGIASGLTKEEVITALTD
                     DLYAYEVDQDIQEARAIGLTGVPFFVFDRKYAISGAQPTEAFLNTLNKSYKEWLASNP
                     QPGLQITSGESCSTKGCDN"
     gene            complement(2745..3674)
                     /locus_tag="PKU82_10535"
     CDS             complement(2745..3674)
                     /locus_tag="PKU82_10535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ83350.1"
                     /translation="MKDISIIIPIYNEENNIHVLHERLTAVVSQMSNSYELIFINDGS
                     RDNSLALVKALAANDNHVYYINFSRNFGHQIAVSAGLDFCTGKAVAIIDADLQDPPEL
                     IQDMYEKMKEGFEVVYAKRRSREGENFMKKFTARIFYRLLARITSVEIPVDTGDFRIM
                     DRKIVDVLKHMPEQQKYLRGQIPWIGFRQTYVEYDRKERLAGETGYTYRKMMRFAIDG
                     ITSFSTAPLKFATASGFVVSFISFIWILYALYSRFIIKDYVPGWTSIMIAVLFIGGVQ
                     LICIGIIGEYISRMSANIRNRPLYVVHDSNIQE"
     gene            complement(3703..4284)
                     /locus_tag="PKU82_10540"
     CDS             complement(3703..4284)
                     /locus_tag="PKU82_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HOZ83351.1"
                     /translation="MFKLKLPTDPRWVNIVETNILEILADHAWCEQKAASNAISLIVR
                     YPEYTDMVKVLCDIAREEMEHFRMVVEKMEQRGWTLGFDRKDDYVNRIYQFQRKGGTR
                     NQRMVDHLLFAAMIEARSCERFKVLSENIKDNDLAAFYRELMISEANHYTTFINFARQ
                     YATDDDVEKRWEDYLEFEAEVIAQYGKKETVHG"
     gene            complement(4291..>5215)
                     /locus_tag="PKU82_10545"
     CDS             complement(4291..>5215)
                     /locus_tag="PKU82_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83352.1"
                     /translation="PCYIRSNDSSVIYCENGWYNTTTDKAFFSKNAYVQNKNQILKGD
                     SMFFDNKTSAGEAFKNVSITDTTEKVVVNADYGNYNGQKGKSFMTGNLLMTRMFEKDS
                     LFMHGDSLISLFDSTGKAISYAVYHNVRMYKKDLQATCDSLIYTAADSLLFLYHNPVL
                     WNEANQLTADTISMLISSDGIQKLYLNQSAFITSKEDSIRFNQLRGKTMVGYFADNML
                     SKIDVFGNGQSIYFARNKKNELTGVNRADCSNMIIIIKDSKISKLKLIEKPDATFYPI
                     GELSASELMLKGFNWQESKRPVDKNDIFRKN"
BASE COUNT         1580 a         1126 c          883 g         1626 t
ORIGIN      
        1 tctgaattat caaattatca aattacctaa ttttcaaatt atctaagtac ttcatcattc
       61 agaaaaaatc tacattcctc cgctcacccc caacccctga aggggagtca acccgctaaa
      121 catacttcta tccatagcat taaatctttt ctacttaaaa aattatttgt gtgcaggaac
      181 cctttaggca ttttgcaaag agcaacgcct cttttggcta cggatgcgtg taagcattct
      241 taattcttaa ttcttaattc ttaattcact atcaccttca ctctttctat ttcactccct
      301 cgttttaata tgcaattata aattcctccg ctcaacccca acccctgaag gggagtcaac
      361 ccacctactt catctcttgg agtgaggctc accctcttaa actgactcca cgcactcaca
      421 taatccttat acaccaactt acccattaca tcataaatct ctaactcacc gctcatgctt
      481 tttacatcaa actgcaaggt aaaattccca tcactcggat ttggcatggc agcaactccc
      541 cctttaggcg gctgaggggt aggcagccct accgttggcg cacacaagca cccaagcgtg
      601 ctgtcgcact ccaaaaaata attagggtgg ttaggcactg tattaaagta ataagcgggc
      661 acctgcacac tgtgttggtt aacattgcaa cccatgccca ccacatcagg gttatctatc
      721 gtatgccagt gcaccgtgcc atttcctgtg cttatgtaaa ccttgccatc aggagccaag
      781 cctgcattgc tgaataaagt ttgtaaagaa ggacaagtcg ggcaattagg tggaacagac
      841 ataaatccat catacgtggc caccgtctgc acgcttgcag caatgttggc tgctgttaaa
      901 tcaaactgat atacataaaa tacatttgat acatacagca catctgagtt tggtgaaaat
      961 gccaagccca cattataccc tccgacaaac gatattgcaa tatgtaccgg attgctcaac
     1021 tgccctgtgc aacggttaaa gtcaaatata tccaaccctc catttaaata aaagtatgca
     1081 aaccgactgc cgtcaggcga aaaccatgcc tgtccggcat cttgtggtct aattgcccct
     1141 atattttgtg aataaggtcc cagaatcagg ttattggtgg ttacaagaaa gcgaaaaaat
     1201 ttattggtgt ttatttggtg tgtcataacc caccagtccc gtccattggc atgtttgcag
     1261 gctgttattt ttccggtaac cattgaatcg gtaagtaaaa tctgattttt agatattact
     1321 gtacccaaac ctccgtttaa gctcatgtca atggtggtta ggtaaaaata aagagagtaa
     1381 gcaccgggta cagggtgatt atccagcgtg ttatgaaaca tataataaat tgaactgctt
     1441 ccgggttggc gtataatcag tgcaccttgc ggtatttgaa acccgtctga aaactggtct
     1501 gcatacgcac ccggatttat cccgcttcca ttctgcatgg tatcattgct ggcatcggct
     1561 atgtaatagc cgttggtgta aaacaataaa ttaccttgtg catcacttat attggcatgg
     1621 gtatgtctaa agtccatttc caaactatcg taattaataa ttggtattcc attaaaaaaa
     1681 tcaaacttag tgttgccaaa aggcaatcct ccaaagctgg agtatcccat cagccagtta
     1741 ttatttattc cctgttgggc agtactaatt tttactgcaa tcagtaaaat gaataaaatg
     1801 tattttttca ttttataaaa atgcccggtt taaaatgagt tattattagt tccttatcac
     1861 aaaattgaat tgtaaaaata caatgtaatc aagaataaaa caatgcagaa tgtagatttc
     1921 agaattatca aacttatcag ttgtggctga atactaaaaa aactattttc acaattcatt
     1981 gtttatccgt cttaataaaa ttctaaaata gaaatgataa ttgaaatctg gagcgacatt
     2041 atgtgtccat tttgctacat aggcaaacgc cactacgaaa atgcattaaa acaatttgcc
     2101 ggtaagaata atatagagtt gaagtggaaa agttatttac ttgatcccga aactcctctt
     2161 gaatttgaaa agccgcttaa tgtctatgaa tatcttgccg aaagaaaagg catcagcctt
     2221 gcacagtcca taaaaatgca tgaacatgtt gtggcaatgg ctaaacaagc aggattggat
     2281 tatcaattag acaaattagt aattgccaat atggttaaag cccatcgcgt tattcagttt
     2341 gctaaaaaga aaggacttgc cgacctgatt gaagaaaata tgttcaaagc ctattttaca
     2401 gaggggaaaa accttgctga tgatgatgta ttagttgcta ttggcattgc atcaggttta
     2461 acaaaagaag aagttattac tgcattgaca gatgatcttt atgcctatga agtagatcag
     2521 gatattcagg aagcaagagc gattggatta accggtgttc cgttttttgt ctttgacaga
     2581 aagtatgcta tttcaggtgc gcaacccaca gaagcatttc tcaacacatt aaacaaatcc
     2641 tacaaggaat ggcttgcatc taatccacaa cccggcttac aaataacctc cggagaaagt
     2701 tgcagcacaa aaggttgtga caattaaaac ttttcttcaa gcaatcactc ttgtatgttg
     2761 ctgtcatgca ccacatacaa aggtctgttt ctgatattgg cactcatgcg gctgatgtat
     2821 tctcctatta ttccgataca aataagttgc acacctccaa taaataaaac agctatcata
     2881 atagatgtcc atcccggaac ataatcttta atgataaacc gcgagtaaag cgcatacaat
     2941 atccagataa aagaaataaa agaaactaca aaaccactgg cagtagcaaa ttttaatggc
     3001 gctgtcgaga aagatgtaat gccatcaata gcaaagcgca tcatctttct gtaagtatat
     3061 cctgtttcac ctgcaagccg ctctttgcgg tcatattcta cataggtctg ccggaaacca
     3121 atccacggaa tctgtccgcg caaatatttc tgctgctcag gcatgtgttt tagcacgtca
     3181 acaattttac ggtccataat gcgaaaatcg cctgtgtcaa caggaatttc aacagaggta
     3241 attcgggcaa gaagtctgta aaaaatcctt gccgtaaatt ttttcataaa gttctcccct
     3301 tctctgcttc ttctttttgc ataaacaacc tcaaagcctt ctttcatttt ctcatacatg
     3361 tcctgtatca actccggtgg gtcttgcaga tcggcatcta ttatggcaac agctttaccc
     3421 gtgcagaaat caagacctgc actaactgca atctgatgtc cgaagttgcg gctgaaattt
     3481 atatagtaaa cgtgattgtc attagccgca agtgctttta ccaatgcaag gctgttatcg
     3541 cggctgccat catttataaa tatcagctca taactgttgc tcatttgcga aactacagca
     3601 gtgaggcgct catgcaacac atgtatgttg ttctcctcat tataaatagg aataataatc
     3661 gaaatatctt tcataacact acttgtctta ctgtcatttt atttacccgt gtacagtttc
     3721 ttttttacca tattgagcaa tcacctctgc ctcaaattca agataatctt cccaacgttt
     3781 ttctacatcg tcatctgttg catactgcct tgcaaaattt ataaatgttg tgtagtgatt
     3841 ggcttcactt atcatcagct ctctgtaaaa tgcagccaaa tcattgtctt taatattttc
     3901 gctaagcacc ttgaatcgtt cacagcttct tgcttctatc attgctgcaa aaagtaaatg
     3961 gtcaaccatt ctttgattac gggttccgcc tttgcgctga aactgataaa tacggttcac
     4021 ataatcatcc tttctatcaa agcctaatgt ccatccacgc tgttccattt tctcaaccac
     4081 cattctgaaa tgctccattt cttctcttgc aatatcacat agcactttca ccatgtctgt
     4141 atattccgga taacgaacta tcagcgatat ggcattgctt gcagcctttt gctcgcacca
     4201 ggcatggtca gcaagaattt caagaatgtt tgtctcaact atatttaccc atcgcgggtc
     4261 tgttggcaat tttagtttaa acattatact ttaatttttt ctaaagatat cattcttatc
     4321 cacaggtctt ttactctcct gccagttaaa accttttagc atcaactccg aagccgataa
     4381 ctcaccaata gggtaaaacg ttgcatcagg tttttctata agtttaagtt tgcttatttt
     4441 gctgtcttta ataattataa tcatgttgct gcaatcggca cggttgacac ccgtaagttc
     4501 gttcttttta tttcttgcaa aataaatgct ctgtccattg ccaaaaacat caattttact
     4561 caacatgttg tctgcaaaat atccaaccat agtttttcct ctaagctgat taaacctaat
     4621 gctgtcttct tttgaagtga taaatgccga ctgattaaga tataattttt gaataccgtc
     4681 agatgaaata agcattgaaa tagtatcggc agttagctga tttgcttcat tccacaacac
     4741 cggattgtga tataaaaaca acaaactgtc ggcagcagtg taaatcaacg aatcgcaggt
     4801 tgcctgcaaa tcctttttat acattcttac gttgtgataa acagcatacg aaattgcttt
     4861 acctgttgaa tcaaacaatg agataagact gtcgccatgc ataaacaaac tgtctttttc
     4921 aaacattctt gtcatcagca ggtttcctgt cataaaagac ttaccttttt gtccattata
     4981 gtttccatag tcagcattaa caacaacttt ttctgttgta tctgtaatgc ttacattttt
     5041 aaaagcttct cctgctgagg tcttattgtc aaaaaacatg ctgtcgcctt tcagaatctg
     5101 atttttattc tgcacataag catttttact gaagaaagct ttatcggtag ttgtgttgta
     5161 ccatccgttt tcgcaataaa taacagaact gtcgtttgag cggatgtagc aaggg
//
LOCUS       DAOOOL010000159         5197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1722_length_5197_cov_61.428044, whole genome shotgun sequence.
ACCESSION   DAOOOL010000159 DAOOOL010000000
VERSION     DAOOOL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1722_length_5197_cov_61.428044"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKU82_10550"
     CDS             complement(<1..365)
                     /locus_tag="PKU82_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HOZ83353.1"
                     /translation="MAEIVRMPKMSDTMTEGVVAKWHKKIGDQIKSGELVAEIETDKA
                     TMEFESYQNGTLLYIGVEEGKAAPVDSILAILGKPGEDFKPLLEAESKAKTATAAVPE
                     KTEAKPAAKVVAETPVKTVA"
     gene            complement(394..1392)
                     /gene="pdhA"
                     /locus_tag="PKU82_10555"
     CDS             complement(394..1392)
                     /gene="pdhA"
                     /locus_tag="PKU82_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOZ83354.1"
                     /translation="MAKQKFTKETYLFWYEQMLLMRRFEERSGQLYGMQKIKGFCHLY
                     IGQEAVVAGAMSVIKPTDNMITAYRDHAHALGKGTSARAVMAELFAKATGCSKGKGGS
                     MHLFDAANRFFGGHGIVGGQIPLGAGIAFAEKYKGTDSVTLCYMGDGAVRQGAFHEAL
                     NMAMTWKLPVVFIIENNNYAMGTSVERTSNVTDLYELGTAFNMPSEPVDGMMVETVHE
                     AMDKAVSHCRKGNGPYLLEMNTYRYKGHSMSDPAKYRSKEELEKYKAQDPIEEVLKVI
                     QKNKLATQEEIDAIDKKVHDIVEDSVTFAEESPFPDVDELYKDVYEQSDYPYIIED"
     gene            complement(1433..1927)
                     /gene="cdd"
                     /locus_tag="PKU82_10560"
     CDS             complement(1433..1927)
                     /gene="cdd"
                     /locus_tag="PKU82_10560"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOZ83355.1"
                     /translation="MMQKIQLTSSYEEYNSLNELPADYKSLMLEAHEAAETAYAPYSD
                     FHVGAAVLLANGEIIKGSNQENAAYPSGLCAERVAVFASAVQYPGVKIIAIAVTAMPG
                     SQEEPVMASPCGACRQVMAEYEHLYHQDIKFIMYGSENKIIVLDSIKALLPFSFNSDQ
                     LSGK"
     gene            complement(2042..3388)
                     /locus_tag="PKU82_10565"
     CDS             complement(2042..3388)
                     /locus_tag="PKU82_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83356.1"
                     /translation="MKALAGYFSLWLKKNTFLLWALAVIHLIFFLLALRNGNIAFSVD
                     STEYLNQAENIREHASWYAGSSSTPHDDFLESRRPPLYGFFIFLVHIVSSNNFFLLAL
                     QNLMSIAVLILTAFMFWLLTGKRIANQWLVILPLLLFPTQFIYANMIMADVLFQFLII
                     SGFLFFIQSVRRPDSFLYFCIFISLAILTKPVFYAFCLIVPVIALLFYTKGIYKLSNV
                     YISLIPLLTVATISTINYSKTGYFHYSSVNEKFISEYGAYLAVGDKGDISAQQKIDSL
                     LSEANKQPDFKSYCKYINKESLFLIKENKARFVMMQLKGMANFFIDHGRFDLMAFFVN
                     PDYVQTEGWKQKYEEQGWQGVFSYLKTFNPFLLLFLILASVFNLIMVVVLVRFLFAHK
                     IAILFRLLLLLFVIYMVVFTGVVGCSRYRMAIFPIIWMAFMWFLTERKSVHHKTVV"
     gene            complement(3408..>5197)
                     /locus_tag="PKU82_10570"
     CDS             complement(3408..>5197)
                     /locus_tag="PKU82_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83357.1"
                     /translation="KFGNINQPPYYDSCQATVNGSQGFFAFATDSAYSVGGNTVYRNL
                     DSLANFINRIPTGNYVVLYSVTEHFMSKSWSGQFRFALSQQGINNVDTIPDTKHFIFF
                     FKKGFPGTAQTVVAATDSTKNITFTTMLGGNWYKGYMMSEIIGPAIQWNSLHWANNSV
                     EAINTDTLALDIVGVDTLNSEHVLYSAIQPSQIDFILNINPFIYPRIYLRAYMEDSVY
                     RTPPQLNRWQIYYQEVPEAVLNPVGSSFQSNSVSQGDTIHWKMPVENVSLSDMSDLLV
                     DYYLFDKDNIRRNITSTRFHPLLHGDTLNAAVKFSTAAYPGLNSLWMEVNPRNDQPEQ
                     YHFNNYARIDFNVNRDVTNPILDVTFDGQHILNGDIVSAKPQILIKLKDENRYLALND
                     TSKFKVYVKSPDGVESQIYFETSANSALSEKLLRWTPAQLPDNTFKIEYNPIFNVDGI
                     YELRAQARDESGNLSGSNDFRISFEVINKSSITNVFNYPNPFTTRTQFVFTLTGSEVP
                     TYFKIQIYNISGKVVKEITQQQLGNIHIGRNITEYAWDGKDEFGDQLAKGVYLYRVIT
                     QLDGDDIEHRNTTADKYFRKGWGKMYLLK"
BASE COUNT         1619 a         1101 c          834 g         1643 t
ORIGIN      
        1 gcaacagttt ttaccggagt ttcagcaaca accttcgctg caggttttgc ctcagtcttt
       61 tccggcacag cagctgttgc cgtttttgct tttgactcag cttcaagcaa tggcttaaaa
      121 tcttcacccg gcttacctaa tattgctaat atactatcaa caggcgcagc ttttccttct
      181 tcaacaccaa tataaagtaa tgttccgttt tgataacttt caaactccat agtggcttta
      241 tcggtttcaa tctctgcaac caattcgcca gactttatct ggtcaccaat ttttttatgc
      301 catttagcaa caacgccttc tgtcatggtg tcgctcattt tgggcattct aactatttct
      361 gccatgtttg attttacttg taatgcttgt taattaatct tcaataatgt aaggataatc
      421 ggattgctca tagacatctt tatacagttc atctacatca ggaaatggtg attcttctgc
      481 aaaggtcaca ctgtcttcaa caatatcgtg aacttttttg tcaatcgcat ctatctcttc
      541 ctgtgttgca agtttatttt tctgtattac tttcagtact tcttctatgg gatcttgtgc
      601 tttatatttt tccagctctt ccttacttct gtattttgca ggatcactca tagagtgtcc
      661 tttataacga tacgtattca tctctaacaa atacggacca tttccttttc tgcaatgact
      721 tactgcttta tccatagcct catgcacagt ttctaccatc ataccatcaa caggttcgct
      781 gggcatatta aatgcagtac ctaattcata taaatcggta acattactgg ttcgttctac
      841 agaagtaccc atagcataat tattattctc gataataaaa actaccggta gtttccatgt
      901 cattgccata ttcaatgctt catgaaatgc accttgacgc actgcaccat cacccatata
      961 acataaagtc acactatctg tacctttata tttttcggca aatgcaatgc cagcacctaa
     1021 tggaatttgt ccgccaacaa taccgtgtcc gccaaaaaaa cgatttgcag catcaaacaa
     1081 gtgcatactt ccacccttac ctttactgca tccggtagct ttagcaaaca gctctgccat
     1141 gactgctctt gccgaagttc cttttcctaa agcatgtgca tgatcacgat atgctgtaat
     1201 catattgtcg gtaggtttga taactgacat tgcccccgca acaacagctt cctgtccaat
     1261 atacaaatga caaaatcctt ttatcttttg cataccatat aactgtcctg aacgttcttc
     1321 gaacctgcgc atgagcaaca tttgttcata ccaaaaaaga taggtttcct ttgtaaattt
     1381 ctgtttagcc atattcttta taatgctaca aaaatagtta aatactgttt gcctatttcc
     1441 ctgatagttg atctgaatta aacgaaaaag gcaatagggc ttttatactg tcgagtacaa
     1501 ttattttgtt ctcagaacca tacatgataa atttaatgtc ctgatgataa aggtgttcat
     1561 actctgccat tacctgacgg catgctccac aagggcttgc catcacaggc tcttcctgac
     1621 ttccgggcat agctgttaca gcaattgcta taattttaac accgggatac tgaacagctg
     1681 atgcaaaaac agccactctc tctgcacaca ggccggaagg atatgcagca ttttcctgat
     1741 ttgagccttt aattatttct ccatttgcta acaacaccgc tgctcctacg tgaaaatcag
     1801 agtaaggcgc ataagctgtc tctgccgctt catgtgcttc caacatcaga cttttataat
     1861 ctgccggcaa ttcgtttaac gaattgtatt cctcgtaact gcttgttaac tgaattttct
     1921 gcatcataaa tattttctaa tcagatggct aaattaagca atcgctgaaa ttaaaagctg
     1981 tcattttatt ggtttgaaag taacctaaaa aatcaggtct aaaaaaatca tcatcttggt
     2041 tttaaactac tgttttatga tgtacagatt tcctctctgt aagaaaccac ataaatgcca
     2101 tccagattat tggaaatata gccattcgat agcgactgca tccaacaact ccggtgaaca
     2161 ctaccatata aattacaaac agcaatagaa gtaaacggaa cagaatagct attttatgtg
     2221 caaataaaaa tctgactaat actaccacca taatcaggtt aaagacacta gccaaaatca
     2281 gaaacagcaa taaaaaagga ttaaatgttt taagataact gaacacacct tgccatcctt
     2341 gctcctcata cttctgcttc cacccttctg tttgcacata atccggattc acaaaaaatg
     2401 ccataaggtc aaacctgcca tgatcaataa aaaagttagc catacccttt aattgcatca
     2461 tcacaaaacg tgccttattc tctttaatca gaaacaaact ttctttatta atatacttac
     2521 aataactttt aaaatcaggt tgcttattgg cttcactaag caaagaatca atcttttgct
     2581 gtgcacttat atctccttta tctcccactg ccagataggc accatattca gagataaact
     2641 tttcatttac gctgctgtaa tgaaaatacc cggtcttgga atagttaatt gttgaaattg
     2701 tggctacagt caataatgga atcagtgata tataaacatt gctcaactta taaatgccct
     2761 ttgtataaaa caacagtgct atgactggaa ctatcaaaca aaaggcataa aaaaccggct
     2821 ttgttaaaat agccaacgat ataaaaatac agaaatacaa aaatgaatca ggccttctta
     2881 ccgattgaat aaagaacaaa aatccgctga taatcaaaaa ctgaaacaag acatcagcca
     2941 ttatcatgtt tgcataaata aattgtgtcg gaaacagcaa taagggtaat attaccaacc
     3001 attgattggc aatccgcttt ccggtcagca gccaaaacat aaaggctgta agtataagca
     3061 ctgcaatgct cattaaattc tgcaatgcca gtaaaaagaa attatttgat gaaactatat
     3121 gcactaaaaa aataaagaag ccatataacg gaggccttcg gctttctaaa aagtcatcgt
     3181 gtggtgttga tgaactacca gcataccatg aagcatgttc gcgtatgttt tctgcctgat
     3241 ttaaatattc tgtactgtca acagaaaagg ctatgttgcc atttcgcaaa gccagaagaa
     3301 aaaatatgag atgaatcaca gccaaagccc aaagaagaaa tgtgttcttc ttcaaccata
     3361 atgaaaaata accggcaagg gctttcatag gtctttaaaa ggttgcctta ctttaagaga
     3421 tacatttttc cccatccctt tctaaagtat ttgtcagcag tggtattccg atgctcaata
     3481 tcatcaccat caagttgtgt tattactctg taaagataaa caccttttgc caactgatca
     3541 ccaaattcgt cttttccatc ccatgcatat tctgtaatgt ttcgaccaat gtgaatgttt
     3601 cccaattgct gttgtgttat ttctttcacc actttgccac tgatattata gatctgaatt
     3661 ttaaaatagg taggcacttc cgatccagtg agtgtaaaca caaactgtgt tcgtgttgta
     3721 aatggatttg gatagttgaa cacattggta atgctactct tattaattac ctcaaaagaa
     3781 attcgaaaat cgttgctgcc gctaaggtta ccactttcat ctcttgcctg agcacgaagt
     3841 tcgtaaatgc cgtcaacgtt gaaaatcgga ttgtactcta tcttaaatgt attatcagga
     3901 agttgcgcag gtgtccaacg tagtaatttc tctgacaatg ctgaattcgc tgatgtctca
     3961 aaataaatct gactctctac accatcagga ctttttacat acactttaaa ttttgaagtg
     4021 tcatttaaag ccaaatacct gttttcatct tttagtttaa tgagtatttg cggctttgca
     4081 gaaacaatgt cgccattaag aatatgttgt ccatcaaagg taacatcgag aattggattt
     4141 gtcacatcac ggtttacatt aaaatctatt cttgcataat tattgaaatg atattgctcc
     4201 ggttgatcat ttcgcggatt tacctccatc cacaatgagt ttaatcccgg ataagctgct
     4261 gtgctgaact ttaccgcagc attcaatgtg tcgccatgta acaaaggatg aaagcgtgta
     4321 gaagttatat ttcgtcttat attatcttta tcaaacagat agtaatccac aagcagatca
     4381 ctcatatcag aaagacttac attctctaca ggcattttcc aatgaatggt atcgccttgt
     4441 gaaacactat ttgattgaaa agaagaacct acaggattca acacagcttc cggaacttcc
     4501 tgataataga tttgccaacg attgagttgc ggtggtgtac gataaacaga atcttccatg
     4561 tatgcacgca aataaattcg cggataaata aacggattaa tattcagaat aaaatctatc
     4621 tgcgatggct gaatagcgct gtacaaaaca tgttctgaat ttaatgtatc tacaccaaca
     4681 atatcaagtg ccagcgtatc tgtattgata gcttccacac tgttgtttgc ccaatgcaaa
     4741 ctgttccatt gaatggcagg accaataatc tctgacatca tatagccttt ataccaattt
     4801 ccaccgagca ttgttgtgaa tgtaatattc tttgtactgt ctgtagctgc aacaactgtt
     4861 tgtgccgtac ccggaaatcc ttttttgaaa aagaaaataa aatgtttagt atcaggaatt
     4921 gtatctacgt tattaattcc ctgttgcgat aatgcaaagc gaaactgccc tgaccatgac
     4981 ttgctcataa aatgctcagt cacgctgtat aataccacat agtttcctgt tggaatacga
     5041 ttgataaaat tcgcaaggct gtcgagattg cgatatacag tgttgccacc aacgctatat
     5101 gcagagtctg ttgcaaaagc aaaaaatccc tgactgccat ttactgttgc ctgacaagaa
     5161 tcgtaataag gaggttgatt gatgttacca aattttc
//
LOCUS       DAOOOL010000160         5184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1728_length_5184_cov_54.894131, whole genome shotgun sequence.
ACCESSION   DAOOOL010000160 DAOOOL010000000
VERSION     DAOOOL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_5184_cov_54.894131"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKU82_10575"
     CDS             complement(<1..682)
                     /locus_tag="PKU82_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOZ83358.1"
                     /translation="MIVLLLNFCTTQQKQTLPLLSSYASLNDTVKYVGKETCRSCHAD
                     IYNTFMQTGMGKSFDVASKQKTSAKFSHAVIYDKYENFYYHPYWQNDSLHLLEFRLEN
                     GDTVYKHNEVISYIIGSGQHTNSHIINTNGYLNQAPATFYTQRGEWHLPPGFEDGNNT
                     RFSRIIGLECMSCHNSYPDFVLGSENKYNKIESGINCERCHGPGEYHVQQKQAGILVD
                     TAKSIDFSI"
     gene            complement(767..1285)
                     /locus_tag="PKU82_10580"
     CDS             complement(767..1285)
                     /locus_tag="PKU82_10580"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HOZ83359.1"
                     /translation="MEELRYPIGKFQLPENITKSQFDSWKNDIAGLPKKVQQLTDSFK
                     SHHWSQTYRQEGWTALQLVHHLADSHINCFIRFKWALTEENPTIKAYDEKAWANLPDV
                     TQSSPVISIQLLHALHQRWINLINNMTEQDFEKTFFHPIQQKTHSLKGAVGLYAWHGN
                     HHLAHLKIIADK"
     gene            1434..3221
                     /gene="lepA"
                     /locus_tag="PKU82_10585"
     CDS             1434..3221
                     /gene="lepA"
                     /locus_tag="PKU82_10585"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOZ83360.1"
                     /translation="MENIRNFCIIAHIDHGKSTLADRLLEYTNTVSKQQMQAQVLDDM
                     DLERERGITIKSHAIQMDYTLDGKKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQGIQAQTISNLYLALEHDLTIIPVLNKIDLPSAEPELVKDQIVDLLGCRREEIMAA
                     SGKTGMGVHDILREIVHRIPAPKGNPKAPLKALIFDSVYNSFRGIIAYFKVIEGEMKS
                     GDKVKFVATNSNYIAEEIGILKLDKIPAKTIGTGNVGYIITGIKDAKEVKVGDTITTL
                     ENPCSEAISGFEDVKPMVFAGIYPVDTEDYEELRSSMEKLQLNDASLVFEPESSAALG
                     FGFRCGFLGMLHMEIIQERLEREFNMTVITTVPNVSYVSYLTNGDVYEIHNPSDFPDP
                     SKMDHIEEPYIKAQIITKAEYIGAIMTLCINKRGVIIGQNYLTTDRVELMFEMPLAEI
                     VFDFYDKLKSVSKGYASFDYHPIGYKTSDLVKLDIRLNGEPVDALSALIHRDHSYTFG
                     KKICEKLKELITRQQFEIIIQASIGAKIIARETVKAIRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQVGSVEIPQQAFLAVLKLD"
     gene            complement(3252..3908)
                     /locus_tag="PKU82_10590"
     CDS             complement(3252..3908)
                     /locus_tag="PKU82_10590"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOZ83361.1"
                     /translation="MENRKISNKGSATLFQNRLLERLTRTTFYFPVILFLLVGVATAL
                     YCYLSLKINVFIIVALFVAGLFAFTLVEYLIHRFVFHFNASTPGQELLKYKIHGVHHH
                     FPKDKDRLAMPPFMSLTLAVLFFLLYKWLFGNYGIALFSGFVSGYSVYLIIHYAVHRY
                     RQPRNFLSILWKHHSLHHYRSDNSAFSVSNPMWDYIFGTMPDKKSIQKGDKEKLPDSL
                     "
     gene            complement(3928..4821)
                     /gene="speB"
                     /locus_tag="PKU82_10595"
     CDS             complement(3928..4821)
                     /gene="speB"
                     /locus_tag="PKU82_10595"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOZ83362.1"
                     /translation="MKALSTKHNFLGIEDEKYYNYESSKYVIQQVPYEHTSSYLAGSD
                     KGPAAIVKASQYVELYDEELDAETYKTCGIATLKALDFKNKTNKKAVDYIEKETLALL
                     NDNKFVVSLGAEHTVTLGFVKAHIQKFKNVSVLQIDAHSDLRESYHNNIYSHASVMKR
                     IFDLGLNIVQAGIRAQCIEEAQLIKKSKRLHTYYAHNIRKDAKWIDKAVKDLSENVYV
                     TIDADGFDPSVIPAVGTAEPNGLFWNETLELLKKTAKEKNIIGFDIVECAPTKGNILS
                     EYTLAKLAYRIIGYIEAARKE"
     gene            complement(4828..>5184)
                     /gene="sucC"
                     /locus_tag="PKU82_10600"
     CDS             complement(4828..>5184)
                     /gene="sucC"
                     /locus_tag="PKU82_10600"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007924.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HOZ83363.1"
                     /translation="MATMDIIKLAGGEPANFLDIGGTANAARVEQAFRIILKDPNVKA
                     ILVNIFGGIVRCDRVAQGIVDAYNNIGQIPVPIIVRLQGTNAEEAKKIIDQSGLKVFS
                     AILLKEAAQLVKEKLG"
BASE COUNT         1617 a          982 c          951 g         1634 t
ORIGIN      
        1 caatagaaaa atctattgat ttagcggtat caaccaatat accggcctgt ttttgctgaa
       61 catgatattc gcccgggcca tgacaacgct cgcagttaat gccactctct attttattgt
      121 atttattttc tgaacccaaa acaaaatcag gataactgtt atgacaactc atacattcca
      181 aaccgattat tcgtgaaaaa cgtgtgttgt tgccgtcttc aaatccggga ggcaaatgcc
      241 actcacctct ttgtgtataa aaagttgcag gcgcctgatt caaataaccg ttggtgttga
      301 tgatatgtga gttggtatgc tgacccgacc ctataatata actaataact tcattgtgct
      361 tataaacagt atcgccattt tcaagtctga attctagcaa gtgcaatgaa tcgttctgcc
      421 agtagggatg gtagtaaaag ttttcatact tatcataaat tactgcatga ctaaatttag
      481 ctgatgtttt ctgtttggaa gcaacatcaa atgatttgcc catgcccgtt tgcataaacg
      541 tattgtaaat atcggcatgg catgaacggc aggtttcttt gccaacatat tttactgtat
      601 cgttcagact tgcataggat gaaagcaatg gcagtgtttg cttttgttgc gtagtgcaaa
      661 aattcaacaa taacactatc attaaaagaa tggaaaaaga aatgagtttt tgcctcataa
      721 acacaaataa aaaccaaaaa tcatcagcaa taaaccatta acaaatttat ttatcagcaa
      781 taattttcaa atgtgccagg tgatgatttc catgccatgc ataaagccca actgcgcctt
      841 tcaggctatg tgttttttgt tgaatgggat gaaaaaaagt tttttcaaaa tcttgttcgg
      901 tcatattatt gataagattt atccatctct gatgcaatgc atgaagtagt tgaatagaaa
      961 tcaccggtga tgattgagtc acatcaggaa ggttagccca tgctttttca tcataggctt
     1021 taattgtagg attttcttca gtcaatgccc acttaaaacg gataaaacaa tttatatggc
     1081 tatcagctaa gtgatgcacc aactgtaagg ctgtccaccc ttcctgtctg tacgtttgcg
     1141 accaatgatg cgatttaaaa ctatctgtta actgttgcac cttttttggc aaaccggcaa
     1201 tatcattttt ccagctatca aactgcgact tagtaatatt ttcgggcaac tgaaattttc
     1261 ctatcggata acgtagttct tccataattt gtcttatttt aatcttgatt tagcattatt
     1321 gaatattctg acaaaagtct gaaaacagaa gcatctttca tttgtaaaaa tatgtcttcc
     1381 aaactttaaa aaaccatggt atgaaggctt aaaaaagcta tttttgccgc ccaatggaaa
     1441 atatcagaaa tttttgcatc attgcccata ttgatcatgg caaaagcacc cttgcagacc
     1501 gtctgcttga atataccaat actgtaagca agcagcagat gcaggcacag gtgcttgatg
     1561 atatggatct ggaacgcgaa cgcggcatca ccataaagag ccatgccatt caaatggact
     1621 atactttaga tgggaaaaag tatgtactga atctgataga tactcccggc cacgttgact
     1681 tttcttatga agtatcaaga agtattgctg cctgcgaagg tgcattatta attgttgatg
     1741 ctgcacaggg tattcaggcg caaaccatca gtaatcttta tttagcgctt gaacacgacc
     1801 ttacaattat ccctgtttta aacaaaattg accttccgag tgcagagcct gaattggtga
     1861 aagatcagat agtagatttg ttaggttgca gacgtgaaga gattatggca gccagtggaa
     1921 aaacaggtat gggcgttcat gatattttgc gtgaaattgt acatcgtatt cctgcaccaa
     1981 aaggcaatcc aaaagctcct cttaaagcac tgatttttga ttctgtatat aattctttca
     2041 gaggtatcat cgcatatttt aaagttattg aaggcgaaat gaaatcgggt gacaaagtaa
     2101 aatttgttgc taccaacagt aattatatag ctgaagaaat tggtattctg aaacttgata
     2161 agatacctgc aaagactata ggcacaggta atgttggcta tatcattaca ggaataaaag
     2221 atgccaaaga agtaaaagtt ggtgatacca tcacaacttt ggaaaaccca tgttcagaag
     2281 ctatttccgg ttttgaagat gttaagccta tggtatttgc cggaatatat cctgtagata
     2341 ctgaagacta tgaggagttg cgctcatcaa tggaaaaact tcagttgaat gatgcctcat
     2401 tggtttttga acccgaatcg tcagcagcat taggcttcgg tttccgttgt ggctttttag
     2461 gaatgcttca catggagatt attcaggaaa gattggagcg cgagtttaac atgacagtga
     2521 ttacaaccgt tcctaacgtt tcttatgtat cgtatcttac caatggtgat gtgtatgaaa
     2581 ttcacaaccc aagcgacttt ccggatccca gtaaaatgga tcatattgaa gagccttata
     2641 ttaaagcaca gattattacc aaggcagaat atattggtgc catcatgact ttatgtatta
     2701 acaaacgtgg tgtcattata ggtcaaaact accttacaac agaccgtgtg gagctgatgt
     2761 ttgaaatgcc cttggcagaa attgtttttg atttttatga caagttgaaa tctgtttcaa
     2821 aaggatatgc ttcgtttgat tatcatccaa taggatacaa aaccagtgat ttggtaaagc
     2881 tcgatatcag acttaatggc gaacctgttg atgcactttc tgcattgatt caccgcgacc
     2941 actcatatac attcggcaaa aagatttgcg aaaaattaaa agagctgatt acccgtcagc
     3001 aatttgaaat tataattcag gcaagtatag gtgctaaaat tattgcacgc gaaacagtaa
     3061 aagctatcag gaaagatgtt actgcaaaat gttatggtgg tgatatttcc agaaagagaa
     3121 aacttcttga aaaacaaaaa gagggaaaga aacgtatgcg tcaggtaggt agtgtagaaa
     3181 ttccacagca ggctttctta gctgttttaa aacttgacta atttcctttt ctaaacattc
     3241 agaaaattac tttacaagga atcaggaagt ttttctttat cccctttctg aatgcttttc
     3301 ttatccggca tagtgccgaa aatataatcc cacatagggt ttgaaacact gaaagctgaa
     3361 ttgtctgaac ggtagtgatg caggctatga tgtttccaaa gtatgcttaa aaaatttcgt
     3421 ggctgcctgt aacgatgaac agcataatga atgataagat agactgaata gccacttaca
     3481 aatccggaga acagcgcaat accataattt ccaaatagcc atttgtacaa taaaaaaaac
     3541 agcactgcca atgttagcga catgaatggt ggcatagcta aacggtcttt atcttttggg
     3601 aagtggtgat gcacgccatg aatcttgtac ttcagcaatt cctgacccgg tgtgctggca
     3661 ttgaaatgaa aaacaaaacg atgtatcaga tattcaacaa gtgtaaatgc aaataagccg
     3721 gcaacaaaaa gtgcaacgat gatgaataca tttatcttta aacttagata acaatataac
     3781 gcagttgcaa ctccgacaag caaaaataaa attaccggaa aataaaaagt tgtacgtgtc
     3841 agtctttcaa gtaaacggtt ctgaaataat gtggcagaac ccttgtttga tattttcctg
     3901 ttctccatac actattgttt taattaccta ctctttcctt gccgcttcta tatagcctat
     3961 tatcctgtag gcgagttttg ctaatgtata ttctgataaa atatttccct tagtgggtgc
     4021 acattcaaca atatcaaagc cgataatgtt tttctctttg gcagtttttt taagaagttc
     4081 taatgtttca ttccaaaata agccatttgg ctctgcagtt ccaacagccg gtattacaga
     4141 tggatcaaag ccatctgcat caatggtcac ataaacgttt tcacttaaat ctttcactgc
     4201 tttatcaatc cacttcgcat cttttctgat gttgtgcgca taataagtat gcagtcgttt
     4261 tgactttttt attaattgtg cttcttcaat acactgtgct cttatacctg cctgcactat
     4321 attcaatccc agatcgaaaa ttcttttcat aacactggca tgagaatata tattgttgtg
     4381 ataagattcg cgcaagtcac tatgggcatc aatctgaagc actgaaacat ttttaaattt
     4441 ctgaatatgt gctttcacaa atcctaaagt aaccgtatgc tctgcaccaa gtgaaaccac
     4501 aaacttattg tcgttaagca gcgcaagcgt ttccttctca atataatcaa ctgctttttt
     4561 attggtcttg tttttaaaat caagagcttt aagtgttgct atgccgcatg ttttatatgt
     4621 ttctgcatct aactcttcgt catacaattc cacatactgc gaagccttta caatagcagc
     4681 cggccctttg tcggagcctg ccagatagga acttgtatgt tcgtaaggca cttgctgaat
     4741 aacatatttg gaagattcat agttgtaata cttttcatct tcaataccca aaaaattgtg
     4801 cttggttgac agggctttca tattctatta tccaagcttt tcttttacca attgtgcagc
     4861 ctcctttaat aaaattgctg agaacacttt tagtcccgac tgatcaatta tcttttttgc
     4921 ttcttcggca ttggtgccct gcaagcgaac aataatcgga acaggaatct gtcctatatt
     4981 attataagcg tcaacaattc cctgagccac tctatcgcat ctcacaatac ctccaaaaat
     5041 atttaccaat atagctttta catttgggtc tttcagaatg atacggaatg cctgttctac
     5101 acgggctgca tttgcagttc ctccaatatc aagaaagttt gccggttcgc cacctgctaa
     5161 tttaataatg tccatagttg ccat
//
LOCUS       DAOOOL010000161         5184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1729_length_5184_cov_37.664262, whole genome shotgun sequence.
ACCESSION   DAOOOL010000161 DAOOOL010000000
VERSION     DAOOOL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_5184_cov_37.664262"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PKU82_10605"
     CDS             <1..937
                     /locus_tag="PKU82_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOZ83364.1"
                     /translation="YKNAGATLGDKAQVYGSDVVLKVNAPQPAEIAQMKKGATLISFM
                     WAATNADLVEACKQAGINAFSMDAIPRISRAQKMDALSSQANLAGYKAVVLCADTLGK
                     ILPMMTTAAGTIRPSKVVIFGAGVAGLQAIATAKRLGAVVEVSDIRPETKEQVQSLGG
                     KFIEVKGDDSIKIEGGYVKGVSEEFLKRQQELVAKHIKEADIVITTALIPGKKAPLLV
                     TEEMVKSMKNGSVILDMAVEQGGNVTGSELNQTVQKNGVTIIGESNIPSLLPMNASDL
                     YAKNIQTFLLHLTTKDGFKWEMEEEITKGSLIVKS"
     gene            1181..1486
                     /locus_tag="PKU82_10610"
     CDS             1181..1486
                     /locus_tag="PKU82_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOZ83365.1"
                     /translation="MTEIINFIGTHQEIIFFIILSVFVGIEVIGGVPTVLHTPLMSGA
                     NAIHGVVIVGAIIVMLNVDANDYVALALGFLAVVLGTLNVVGGFVVTDRMLEMFKKK"
     gene            1518..2954
                     /locus_tag="PKU82_10615"
     CDS             1518..2954
                     /locus_tag="PKU82_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOZ83366.1"
                     /translation="MTPEVLYNIRTITYIIASATFIIGLKRMGNAKTARNGNLIAAVG
                     MTLAIIASIFIHVNKTQTATGVTFEQVETPGFIYTLIFAAIIIGTIIGWLTAKKVQMT
                     KMPELVSMFNGMGGACAALIGLMEFHHNTGNTGALIAIVLGLIIGSVSFSGSVIAFLK
                     LNGTMNKPVRLPSYNIINTLVMIGVFAFGAYMIFSGTTSATLLYLLFAAALIYGILFT
                     IPIGGADMPVVISLLNSFTGLAAAFGGFLYDNKVMLTGGILVGSAGTLLTLVMCKAMN
                     RPLSNVIFGAFGGGGASAGDGPDVKGSIKDMSVADAAVLLNYSKKVVIVPGYGLAVAQ
                     AQHIIHELEMLLEERGVEVKYAIHPVAGRMPGHMNVLLAESNVDYGKLVEMEEINPEL
                     EQTDVVLVVGANDVVNPAAKTDPSSPIYGMPILEVDKAKHIIVNKRSMNAGYAGIDNL
                     LFYDPKTSMLFGDAKKALTELVAELKTM"
     gene            complement(2982..5129)
                     /locus_tag="PKU82_10620"
     CDS             complement(2982..5129)
                     /locus_tag="PKU82_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOZ83367.1"
                     /translation="MLSKVKLIKVFLRLSAFICLFFSSDFAVANKVDTLSFFGSKSPK
                     RDTVFSINALSNFEWNVEQYNKNGLTKKYYTDQVDTIQIQNKIPEGALLTTVFYVDSS
                     MLNKVQALFYNTNGSVKISINNEVVALCGVFDGNKHYTDVKSEQKDYKYFILKKNKNV
                     LQVQYLPALKRHNLNIRLGDAKWAAGNLKDKKDGEIRSYAVATFYFSFAIVLILFYFF
                     IKNQEYLFFGLYCLATFLQYLSEVLTQTPTIKVLSAYSFLISLELLAIFMALAINKIH
                     KTKIPLFLMIAVLIFSLIFPDDFIVTIKFVKNIEIPVIRFLSYVIFLSYSLFSFAYHW
                     IQGFGHKNWDAKLLTYGTGIGILLFLLPIGYAMNKSFDQQNISDNILNTLFTISAGVY
                     PITVAIVLARRYGKNQTQLIEQVQTIKKLGEENLLQETEKKNILAQQNILLENKVTER
                     TFELEEKNKEILNKNKEITDSLIYAKRIQAAVLPNLNTVYKILDQSFILYLPKDIVSG
                     DFYEFTQKENKHIIAAADCTGHGVAGAFMSIIGSSLIKSIINEKNITAPSGILDALNE
                     GIINSLKQHESETHDGMDISICSFDTKNMSLEFAGANRPLWLIRNDELIVYAADKFPI
                     GGLQIKHNNQFTNHQIKIQPKDTIYLFSDGYADQFGGEHGKKLMTKKLKEILVSIQHL
                     SMNEQKIYLQNYFNEWKGNNEQVDDVLIIGIRI"
BASE COUNT         1696 a          799 c         1008 g         1681 t
ORIGIN      
        1 gtataaaaat gcaggtgcaa cacttggcga caaggcacag gtatatggta gtgatgtagt
       61 gttgaaggtt aatgcaccgc aacctgcaga aattgctcag atgaaaaagg gtgctacact
      121 catttcattt atgtgggctg ctaccaatgc agatttagtg gaagcgtgca agcaagcagg
      181 catcaatgcg ttttctatgg atgctattcc acgtatttca agagcgcaga aaatggatgc
      241 gctaagttcg caggcaaacc ttgcgggata taaagctgtt gttttatgtg cagatacatt
      301 aggtaaaatt ttacccatga tgactactgc tgcaggaacc attcgaccct caaaggttgt
      361 aatatttggt gcaggtgtag caggcttgca ggcaattgct actgcaaaac gtttgggtgc
      421 cgttgttgaa gttagcgaca tcagacctga aactaaggaa caagttcagt cactcggtgg
      481 aaagtttatt gaagtaaaag gtgatgacag tataaaaatt gaaggtggtt atgtgaaagg
      541 tgtttcagaa gagtttttga agaggcaaca ggaattagta gccaaacata tcaaagaagc
      601 agacattgta attactactg cattaatacc gggtaagaaa gctccattgt tagttacaga
      661 agagatggtg aagagtatga aaaacggttc agtaatttta gatatggctg ttgagcaggg
      721 aggaaatgtt accggaagtg agttaaatca aaccgttcag aaaaatggtg taacaattat
      781 tggagagagt aacataccaa gtttgttgcc gatgaatgca agtgatttat atgcaaagaa
      841 tattcagaca tttttacttc acctaacaac taaagacgga tttaagtggg aaatggaaga
      901 agagataaca aaaggaagct taatagttaa aagttagaga atttttgaat aaaagaattg
      961 tggagttgat gatttaggaa attgtatagt tttttgtgaa tggaagtaac attaaataaa
     1021 aaaacaaaaa aagaccaatt ttgcagagga attaaaaagg cgaactgaaa tattttcaac
     1081 atcaattata aaattcacaa aatcatttcc ccaaaagtag aattcaatag ctcaaaagaa
     1141 aaaacaactc aattattcag aaaattaata attaaacaac atgacagaga taattaattt
     1201 tataggaaca catcaggaga ttatattttt tataattctt tctgtatttg tgggtataga
     1261 agttattggc ggagtaccta cggtattaca cacacctctg atgtccggtg caaatgccat
     1321 acatggagta gttattgtag gagccataat tgtaatgctc aatgttgacg ctaacgacta
     1381 tgttgcactt gcattaggct ttcttgcagt agtgttaggt acattgaatg tggtaggtgg
     1441 atttgttgta acagaccgca tgttggaaat gtttaagaaa aaataaaacc acaaagaaga
     1501 attaaagata tcagcgcatg acacctgaag tattatataa cattcgtaca attacataca
     1561 tcatagcatc tgctacgttc ataataggtc ttaaacgtat gggtaatgcc aaaactgcac
     1621 gtaacggtaa tcttattgcc gcagtaggta tgaccttggc aataattgca tcaatattta
     1681 ttcatgtaaa caaaacacaa actgcaaccg gtgtaacatt tgaacaagtt gaaacaccag
     1741 ggtttattta cacattaatt tttgctgcaa tcattatcgg aacaatcatt ggctggctta
     1801 ctgctaaaaa agtgcagatg acaaaaatgc ctgaactggt ttctatgttc aatggtatgg
     1861 gtggtgcctg tgcagcgctt attggtctca tggagtttca tcataataca ggaaacaccg
     1921 gtgctttaat tgctattgtg cttgggttga taatcggtag cgtgtctttt tcaggaagtg
     1981 ttattgcatt tttaaagttg aatggtacaa tgaacaaacc tgttcgctta ccatcataca
     2041 acatcattaa cacattagtc atgataggtg tatttgcttt tggtgcatac atgattttta
     2101 gtggaaccac aagtgcaaca ttgttgtatt tactttttgc agcagcattg atatatggaa
     2161 ttttgtttac cattccaatc ggtggtgccg atatgcctgt tgttatttca ttgcttaact
     2221 cattcacagg gttggcagct gcatttggcg gtttccttta cgataataaa gtaatgctta
     2281 ccggtggtat tcttgttggc tcggcaggaa ccttgttgac acttgtgatg tgtaaggcta
     2341 tgaatcgtcc tttgagtaat gtaatttttg gagcctttgg cggtggtggt gcttcagcag
     2401 gtgatggacc tgatgttaaa ggaagtatta aagatatgtc tgttgctgat gcagccgtct
     2461 tacttaatta ctctaagaaa gtagttattg ttcccggtta tggattggca gttgcacaag
     2521 ctcaacacat cattcacgaa ctggaaatgt tgcttgaaga acgtggtgtt gaagtaaaat
     2581 atgctattca cccggttgca ggtcgtatgc ccggacacat gaatgtgtta cttgccgaaa
     2641 gtaatgttga ttatggaaag ttggtggaaa tggaagagat aaatcctgaa ttggaacaaa
     2701 cagatgtagt gttggttgtt ggtgcaaatg acgttgtaaa ccctgctgct aaaacagatc
     2761 catcatcacc aatttacggt atgcctattc tggaagttga taaagccaaa catattatag
     2821 ttaacaagcg atcaatgaat gccggctatg caggaattga taatttactg ttttacgatc
     2881 cgaagacaag catgttgttt ggtgatgcta aaaaagcatt gactgaattg gtggctgaat
     2941 taaaaacaat gtaagaacag tttttcttgg taaaggaatt tttatattct gataccgatt
     3001 atcaaaacat catcaacttg ttcgttgttg cctttccact cattaaagta attctgtaaa
     3061 tatattttct gttcattcat gctaaggtgc tgaatagaaa ctaaaatttc ttttagtttt
     3121 ttagtcatca attttttacc atgttcacca ccaaactgat cggcatagcc atctgaaaaa
     3181 agataaatgg tatcctttgg ctgtattttt atttggtgat ttgtaaactg attgttgtgt
     3241 tttatctgta gtccaccaat cggaaatttg tctgctgcat aaacaattag ttcatcattt
     3301 ctgatcagcc acaatggtct gttggcaccg gcaaattcta agctcatatt ttttgtatca
     3361 aaagaacaga tagaaatatc cattccatca tgtgtttcgc tttcatgttg tttaagcgaa
     3421 ttgataatac cttcgttcaa agcatctaaa atccccgatg gcgcagtaat atttttttcg
     3481 ttgatgatag atttaattaa tgagcttccg ataatactca taaaagcacc tgccacacca
     3541 tgaccggtac aatctgctgc ggcaataata tgtttgtttt ctttttgtgt gaactcataa
     3601 aaatcgccac tgacaatatc ttttggcaga tagagaataa atgactggtc aagtatttta
     3661 taaacagtat ttaagtttgg taggactgcg gcctgaattc tttttgcata aatcagactg
     3721 tcagtaattt ctttgttttt gttaaggatt tctttgtttt tctcttcaag ttcaaaggtt
     3781 ctttcagtta ctttgttttc aagcaaaatg ttttgttgtg ccaaaatatt tttcttttcg
     3841 gtttcttgta ataaattttc ttcgcctaat ttttttatgg tttgaacctg ttctattaac
     3901 tgtgtttgat ttttcccata gcgccttgcc aatacaatag caacagttat gggataaaca
     3961 ccagcactga ttgtaaaaag tgtatttaaa atattatctg aaatgttttg ttgatcaaaa
     4021 cttttattca ttgcataccc aatagggagt agaaaaagta aaattcctat ccctgtgcca
     4081 taagttaaaa gttttgcatc ccagtttttg tgaccgaaac cttgtatcca atgatatgca
     4141 aaagaaaata atgagtaaga gagaaaaatt acataactca aaaacctgat aaccggtatt
     4201 tcaatattct taacgaattt aatggttaca ataaaatcat caggaaaaat taagctgaat
     4261 attaaaactg caatcattaa aaataatgga atttttgttt tatgtatttt gttaatggcc
     4321 aaagccataa aaatggcaag cagttcaagt gaaattaaaa acgagtaggc agaaagaact
     4381 ttaattgttg gtgtttgtgt caggacttca gatagatatt gcaagaatgt agctaaacag
     4441 taaagaccaa agaataaata ttcctggttt ttgataaaaa agtaaaaaag tatcaatact
     4501 atggcaaaag aaaaatagaa agtagccaca gcataacttc tgatttcacc atccttctta
     4561 tcttttagat taccggcagc ccattttgca tctccaagtc taatgttgag attatgcctt
     4621 ttcagagcgg gcagatattg tacttgtaaa acatttttat tttttttgag tataaaatat
     4681 ttataatctt tttgctctga tttcacatca gtataatgtt tattaccatc aaacacgcca
     4741 cataatgcca ctacttcatt attgatagaa atttttacag atccatttgt gttataaaaa
     4801 agtgcctgaa ctttatttaa cattgaacta tcaacataaa aaacagtagt cagtagagca
     4861 ccttcaggta ttttgttttg aatctgaata gtatcaacct gatcggtata atattttttc
     4921 gttaagccat ttttattgta ttgttcaaca ttccattcaa aattacttaa tgcatttatg
     4981 ctgaaaacag tatcgcgctt tggagattta gatccgaaaa aagaaagcgt gtcaactttg
     5041 tttgcaacag caaagtcaga gctgaaaaac aaacaaataa atgccgataa gcgtagaaaa
     5101 actttaatca gtttaacttt tgaaagcata tattaagatt gactgggcga aggtaaagta
     5161 taattattta atcggatata taac
//
LOCUS       DAOOOL010000162         5007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1832_length_5007_cov_53.558966, whole genome shotgun sequence.
ACCESSION   DAOOOL010000162 DAOOOL010000000
VERSION     DAOOOL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_5007_cov_53.558966"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKU82_10625"
     CDS             complement(<1..283)
                     /locus_tag="PKU82_10625"
                     /inference="COORDINATES: protein motif:HMM:NF026155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOZ83368.1"
                     /translation="MPVREKTVNAIWENRALLTDESNKKVIRSVIEDLDKGNIRVAEQ
                     MNNSWRVNEWIKKDVILYFPIQKIEVHHAGPREFYDKIDVKKGYENLGVS"
     gene            369..797
                     /gene="ruvX"
                     /locus_tag="PKU82_10630"
     CDS             369..797
                     /gene="ruvX"
                     /locus_tag="PKU82_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOZ83369.1"
                     /translation="MPRILAVDYGNKRVGLAVTDPMQIIATALTTVHSSKAVEFICNY
                     FNSEEVEEIVVGKPVQMDNTPSEIEKNILLFIKKLIDKCPSAKVIRYDERFTSVMAQR
                     TLLEAGLKKQQRQNKALIDKTSAVILLQSYLEHKRNFLKI"
     gene            794..1354
                     /gene="def"
                     /locus_tag="PKU82_10635"
     CDS             794..1354
                     /gene="def"
                     /locus_tag="PKU82_10635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOZ83370.1"
                     /translation="MILPIIAYGDPVLKKVGSSIDKDYKGLTQLIENMFETMYAASGV
                     GLAAPQVGISIRLFVVDTKPFAEDEPELENFKKVFINPQIIEEVGDEWKFSEGCLSIP
                     GIREDVMRKKIIHLRYQDEEFKTHETVFEGLAARVLQHEYDHVDGKLFVEKLSPFKKQ
                     LIKGKLRDITIGKVDVDYKMRFPIKK"
     gene            1360..1872
                     /locus_tag="PKU82_10640"
     CDS             1360..1872
                     /locus_tag="PKU82_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOZ83371.1"
                     /translation="MLIFLIGFMGAGKSSQAKKLAARLNFESIDLDELIEKTVKQSIS
                     EIFKISGEQHFRKIESETLKTLSEKQNAVIATGGGTPCFFDNMQWMNEKGITVYLKIH
                     PGTVYHRIAPSKSKRPLISHMNDADLMEYIMETMIERAPFYNQAKIQISGLNLKTEDL
                     VSALKPFLKD"
     gene            1932..2441
                     /locus_tag="PKU82_10645"
     CDS             1932..2441
                     /locus_tag="PKU82_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83372.1"
                     /translation="MKTLLCFLLLPTILVAQTFQKNQSEGNEDFIKRIAPHSANEVKY
                     LLSDSFNTSTEKIVYAYSIFEHGNLITNNDSTLTYFLCILVPTSVGALDYNMQQFRMV
                     ASYKKKARIESAEIIKSGREKKLQVYIAEMVRGPGGVPRDVKRTFVYKQEKESRMFTD
                     TFREVLGDY"
     gene            2617..>5007
                     /locus_tag="PKU82_10650"
     CDS             2617..>5007
                     /locus_tag="PKU82_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HOZ83373.1"
                     /translation="MASQFIPVDFNPFEEVIPDNFFTTSEEQREIWTSVQIGGDSASL
                     AYNESVTLFLKGLCNHKHLSEACKNVVQRHQSLRSTFNTSGETFQVHEHVNLEIPYID
                     LTDLSPSDKDARILHFSQREVSIPFDLLNEPVIRFAIFKISNSETALVITAHHIVCDG
                     WSIGIIMQDISKFYSALVNNTKPDLEPVVPFSTYVKRQEERKLNGEYKRTEDYWLNLY
                     SADVPTVDLPVNKARPAARTFSAKRFDITIDAGLVSDLRKLGAKNGSSYVNTLFAAFE
                     VFLFRLTSNPSLVLGLPAAGQSASGMYAMVGHCVNLLPIKSLVDENITFGEYLKARKP
                     LMFDAFDNQEFTMGSLLGKLPLQRDPSRIPLVPAVFNVDLGMTQGVSFEGLNYEYTSN
                     ARKFENFELFVNATGMGDKLIIECTYNTDLFEDEMMQLRMQEFVVLLNGLVEYVDSAI
                     KNLPLLTDDEKRKLFIEWPVVKENKLSNECIHSLIEKQASAHPDTNAIWFNGQTITYR
                     QLNQRANQLAHYLIKSGVKKETLVGICHDRTDDLIVAMLAVLKAGGAYVPLDPNYPAD
                     RIAYILSDAKAPLLISSSKYIDQLGETGTKLVCLDKEVTEISKEKITNPDVEITPDQL
                     AYIIYTSGSTGKPKGVAIEHRNAIALIDWAQTVYSSEELAGVLASTSICFDLSIFEIF
                     FTLSNGGRIVLVKDAMALNALPENAGVTLVNTVPSAIAELLKLKDGVPSSVKTINLAG
                     EPLPVELVQKIYSQTTVKKVYDLYGPSEDTTYSTFTLRKADGPYTIGKAISNSQVYI"
BASE COUNT         1629 a          831 c          957 g         1590 t
ORIGIN      
        1 aactaacacc taagttttca taaccttttt taacatctat tttatcataa aactccctcg
       61 gtcctgcatg atgtacttct attttttgaa tcggaaaata taagatgaca tcttttttta
      121 tccactcatt tacgcgccaa ctattattca tttgctctgc aacccgaatg ttccctttat
      181 ctaggtcttc gataactgag cggataactt tcttgtttga ttcatcggta agtaacgctc
      241 tgttttccca aatagcattg actgtttttt ctcttacagg cataaatatt atttttcagc
      301 tacaaatata accaccttaa tgcattcatt gcccgttgtt ttttattttt gcatctttaa
      361 attcagatat gccgcgcata cttgcagttg attacggaaa taaaagagta ggtcttgcag
      421 ttacagatcc aatgcagatt attgctactg ctttaactac tgttcattcc tctaaagcgg
      481 ttgaatttat ttgtaattat tttaattctg aagaagtaga agaaattgtt gttggtaaac
      541 ccgtgcaaat ggataataca ccatcagaaa ttgagaaaaa tattttgttg ttcatcaaaa
      601 agcttattga taaatgtccg agtgcgaaag tgataagata tgatgaacgt tttacctctg
      661 taatggcaca gcggacattg ttggaagccg gactgaaaaa gcaacaaaga caaaataaag
      721 cattgattga taagacaagc gctgttatat tattacaatc gtacttagaa cataaaagaa
      781 actttttgaa aatatgatat taccaattat tgcctatggc gacccggttt tgaaaaaggt
      841 gggctcatca attgataaag attataaagg tcttacacaa cttatagaaa acatgtttga
      901 gactatgtat gcagcatccg gtgttgggtt ggctgctccg caggttggta tttccatcag
      961 actatttgtt gttgacacca aaccatttgc agaagatgaa ccggagctgg aaaatttcaa
     1021 gaaagtattc attaatccac aaataattga agaggtaggt gatgaatgga agtttagcga
     1081 aggttgtttg agcattcccg gaataagaga ggatgtgatg cgtaaaaaaa taattcatct
     1141 gcgctatcag gatgaagaat ttaaaacaca tgaaacagtt tttgaaggat tggcagcacg
     1201 ggtactccaa catgagtacg atcatgttga tggtaaatta tttgttgaga aattatcacc
     1261 ttttaaaaag caattgataa aaggcaaact tcgtgacatt accataggca aggttgatgt
     1321 tgattacaaa atgcgtttcc caataaagaa atagcagcaa tgttgatatt tctgattgga
     1381 ttcatgggag ccggaaaatc atctcaggca aagaaattag ctgcaagatt aaattttgaa
     1441 agcattgatc tggatgaact cattgaaaaa acagttaagc aatcaatttc agaaattttt
     1501 aagataagcg gagagcaaca ttttcgtaaa attgagagtg aaactttaaa gacattatct
     1561 gaaaaacaaa atgcagtgat tgcaacgggc ggtggcacac catgcttttt tgataatatg
     1621 caatggatga atgaaaaagg cattactgtt tatctgaaaa tacatcccgg cacagtttat
     1681 caccgcattg caccttcaaa atctaaacga cctttgatta gccacatgaa cgatgcagat
     1741 ttgatggagt acattatgga gacgatgatt gaaagggcac ctttctataa tcaggcaaaa
     1801 attcagattt cgggactaaa tcttaaaaca gaagatttgg taagtgcact taagccattt
     1861 ttgaaagatt aaaacaatag catttcagat tgactatttt tttttatgtt tacctttcaa
     1921 aataaaatat tatgaaaaca ttattatgct ttttgctgct tccaaccata ttggttgcac
     1981 aaacatttca aaaaaatcaa agtgaaggca atgaggattt tatcaaacgg attgctccac
     2041 attcggctaa tgaagttaaa tatcttctga gtgattcttt taatacttca acagaaaaaa
     2101 ttgtttatgc ctacagcatt tttgagcatg gcaacctgat aacaaacaac gatagtactt
     2161 tgacctattt tttatgcatc cttgttccta cttctgtagg agcattggat tacaacatgc
     2221 agcagtttcg tatggtggca agctataaaa agaaagcaag gattgagagt gcagagatta
     2281 ttaagtcggg acgggaaaag aaacttcagg tatatattgc agaaatggta cgaggccccg
     2341 gaggtgtgcc aagagatgta aagcgcactt ttgtttataa gcaggaaaaa gaaagcagaa
     2401 tgtttactga tactttcaga gaggttttgg gtgactatta aaaacagttg tccttaatat
     2461 gctgatttat tgctgacgtt ctttttttca gattttcata gttgtgaatg gctacattca
     2521 cccttatttt catatcgctt aacaatttat tgctaaagga aactatttta taatattttc
     2581 gtattgtatt tattaaacca ccaatgacaa aggaagatgg catcgcaatt tatcccggta
     2641 gattttaacc cttttgaaga agttattccg gacaattttt ttaccacatc agaagagcaa
     2701 cgtgaaatct ggacatccgt tcaaatagga ggtgattctg catcattagc ctataacgaa
     2761 agtgttacac tttttctgaa aggactgtgt aatcacaagc acctttcaga agcttgcaag
     2821 aatgttgttc aaaggcatca gtcacttcgc tctactttca atacttcagg tgaaacattt
     2881 caggtacatg aacatgtcaa tcttgaaatt ccatatattg atttaaccga cttaagtccg
     2941 tcagataaag atgcacggat acttcatttt tcgcagcgtg aagtgtccat acctttcgat
     3001 ctattgaatg aacctgtaat aagatttgcc atttttaaaa tttcaaattc agaaacggca
     3061 ttggtcatca cagcacatca cattgtctgt gatggatgga gtattggtat catcatgcag
     3121 gatattagta aattttattc tgctttagtg aataatacga aacctgatct tgaacctgtt
     3181 gtacccttca gcacttatgt aaagcgtcag gaagaaagga aattgaatgg ggaatataaa
     3241 cgtacagaag attattggct caatttatat tccgcagatg ttcctaccgt tgaccttccg
     3301 gttaacaaag ctcgccctgc tgcacgtacc ttcagtgcaa aacgatttga tataactatt
     3361 gatgccggtt tggttagtga tttaagaaaa cttggtgcaa aaaatggaag cagttatgta
     3421 aatacgttgt ttgctgcatt tgaagtgttt ctttttcgtt taacatccaa tccttcattg
     3481 gtattaggac ttcctgctgc aggacagtca gcgtccggaa tgtatgctat ggttggtcat
     3541 tgtgttaatc ttttaccaat taaaagtctt gttgatgaga acatcacttt cggtgaatat
     3601 ttgaaagcca gaaaaccttt gatgtttgat gctttcgata atcaggaatt tacaatggga
     3661 agtttgttag gaaaacttcc actgcaacgc gacccgtcaa gaattccttt agtgcctgct
     3721 gtgtttaatg ttgacttggg tatgactcaa ggagtttctt ttgaaggatt gaactatgaa
     3781 tatacttcaa atgccagaaa gtttgaaaac tttgaattgt ttgtgaatgc aaccggtatg
     3841 ggcgataaac tcattattga atgtacttac aataccgacc tttttgaaga tgagatgatg
     3901 caattgagaa tgcaggagtt tgtagtgctt cttaatggtt tggttgagta tgttgatagt
     3961 gcaataaaaa atttgccatt actgactgac gatgaaaaaa gaaagttgtt tatcgaatgg
     4021 ccagttgtaa aagagaataa acttagcaat gagtgcattc atagcttaat tgaaaagcaa
     4081 gcatcggcac atcctgatac aaatgcaatt tggtttaatg gacaaacaat tacttacaga
     4141 caacttaatc aacgagccaa tcagttagcg cattatttaa taaagtctgg tgttaaaaag
     4201 gaaacattag ttggaatttg tcacgacagg acagacgatt taattgttgc catgcttgca
     4261 gtattaaaag caggtggtgc ctacgtgcct ttagatccaa attatcctgc agatagaatt
     4321 gcctatatct tgagcgatgc aaaagcacct ctcttgattt catcgtcaaa atacatagat
     4381 cagttaggtg aaacaggaac aaagttagtt tgtttagata aagaagtaac tgaaatttct
     4441 aaagagaaaa taacaaatcc tgatgttgaa attactcctg atcagttagc atatattatc
     4501 tacacatccg gatcaacagg aaaacctaaa ggtgtagcta tcgaacaccg caatgcaatt
     4561 gccttaattg attgggcaca gacagtttat tcttcagagg aacttgcagg tgtgcttgct
     4621 tctacatcta tctgttttga cttatccatt ttcgaaatat tttttacatt aagcaacggt
     4681 ggacgcattg ttttagttaa agatgccatg gctttaaatg ccttacctga aaatgcaggt
     4741 gttacattgg ttaacacagt gccttctgca attgcagaac tactcaaact taaagacgga
     4801 gttccttcaa gcgttaaaac aataaatctt gcaggtgagc ctttgcctgt tgaattggtt
     4861 cagaaaattt attcgcaaac tactgtcaag aaagtttatg atttatatgg cccttctgaa
     4921 gatactacct acagtacatt tacattgcgt aaagctgatg gcccatatac aataggaaag
     4981 gcaatttcaa attcgcaggt ttatatt
//
LOCUS       DAOOOL010000163         4914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1876_length_4914_cov_220.336695, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000163 DAOOOL010000000
VERSION     DAOOOL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_4914_cov_220.336695"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKU82_10655"
     CDS             complement(<1..715)
                     /locus_tag="PKU82_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ83374.1"
                     /translation="MIAIENISKSFGNKKVLEDVSCEFDKGKINLIIGGSGQGKTVLM
                     KCAVGLFEVDKGKVLYDGRNFSEMTFGERKPIRQEIGMLFQGGALFDSLTVEQNVTFP
                     LKMFTNMTNAEKRDRANFCLHRVNLENVNHLLPSAISGGMKKRVAIARAIAMNPRYLF
                     CDEPNSGLDPKTSILIDELIKEITEEYDITTVINTHDMNTVMSMGEKIIFIYKGKKFW
                     EGNNEDIMRSENKELNDFVF"
     gene            complement(712..1455)
                     /locus_tag="PKU82_10660"
     CDS             complement(712..1455)
                     /locus_tag="PKU82_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83375.1"
                     /translation="MKGTLFEIGRYFGFIKNLFYKPEKLSIYVRQVFREMVNLGIGSL
                     WLVALTSAFMGAVITIQSAYNLVNPMVPLWAVGIVARDSIIIEFSPTIIMLVLAGKVG
                     SSVASEIGTMRVTEQIDALEIMGINSSGYLVLPKIIAALLICPVVVIISMIVGITGGW
                     VAGDLSGVVPSADYLKGIQNQFLPFNVTFAMIKTVVFAFIITSVPAYHGYYTSGGALE
                     VGQSSTKGVVYSSILILIFDYLLTQLILA"
     gene            complement(1480..2526)
                     /locus_tag="PKU82_10665"
     CDS             complement(1480..2526)
                     /locus_tag="PKU82_10665"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOZ83376.1"
                     /translation="MLKKILITAWIISMATGLQAQQKKSYQVGVIAFYNLENLFDTIN
                     QPEVNDEEFTPTGSNNYTGKVYLDKLGKLSDVLSLIGKDESPDGFSVLGCAEVENESV
                     LKDLVNEPKLKSRNLKIVHYDSPDKRGVDVALLYNPKYFTPKFSEPLFIDLRTPENPE
                     YYTRDILYVYGLYLGEPVHFLVGHWPSRRGGEEASAPSRAKAAAVCKNKIDSITKINA
                     DAKIVLMGDLNDDPLSPSVTKVIKAVGSKDKVERGGMYNPWVDFYTKGIGTLAYNDAW
                     NLFDQIMISSGWLNKKQDGFFFKDAHIFKREFMLQKSGRYKGYPLRTFDFSRYIGGYS
                     DHFPTYLVFLKEVK"
     gene            complement(2598..>4914)
                     /gene="rnr"
                     /locus_tag="PKU82_10670"
     CDS             complement(2598..>4914)
                     /gene="rnr"
                     /locus_tag="PKU82_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOZ83377.1"
                     /translation="ILQSNENMSKKNIKFTKTQLEKLKQELLQVFLQHPRATFNPKQV
                     WRKAEINLPEEYISVVLNADKHYIDETIQSLILELFQEGELLEVQQFRYKLLPAERFI
                     EGNIQVTSKGEGYVMNEGFEEDIQIPAHLMANALNGDKVRISLLAHREGRRQQGEVVE
                     VLSRSRTQFAGTIQHSGSYAFVVCDDPKMHVDIFIPGKQLNGARNGDKVIAEITLWSA
                     DASNPEGKIVRVLGAPGEHTAEMNAIIVEYGLPEAFPDEVEKETLKISDVISKEEIKK
                     RRDFRKVTTFTIDPVDAKDFDDALSIQQLPNGNWEMGVHIADVSHFLKLGSAMDEEAF
                     NRATSIYLVDRVIPMLPEKLSNMVCSLRPNEEKLCYSAVFEMNEKAEVLNEWFGRTVI
                     FSDHRFTYEEAQQVIETNEGPFVKEIGVFHRLAQQLRKERHRKGAISFEKTEVKFRLD
                     EKGNPIGIFLKESKDSNKLIEEFMLLANRRVAEFIGKGVAKGKKDKTQKTFVYRIHDV
                     PVEEKLSDFKTFAAQFGYRISTASGKETAHSLNKMLKDVEGKKEQSMIEQLAIRSMAK
                     AVYSTENIGHYGLAFDFYTHFTSPIRRYPDVMVHRLLDYYMSGGKSVSQQEYEEKCRH
                     CTEREINAADAERSSIKYKQVQYLAARKDEIFKGVISGVTEWGLYVELEDNKCEGMIR
                     LRDLNDDYYEFDQKNFCIVGHRSRKKYSLGDELMVVIKSTDIIKKQVDFMLYDANRLH
                     EVSPFKRSDKKRFPTSGKFRGDKKKGGKRRR"
BASE COUNT         1433 a         1047 c          828 g         1606 t
ORIGIN      
        1 caaaaacaaa atcgtttagt tctttatttt cagagcgcat gatgtcttcg ttgttgcctt
       61 cccagaattt tttgccttta tagataaaaa taattttctc tcccatactc atcactgtat
      121 tcatgtcatg tgtgttgatg acagtagtaa tgtcatattc ctccgtaatt tcttttatga
      181 gttcgtcaat gagaatggat gttttagggt cgaggccaga gtttggttca tcgcaaaata
      241 aatatcttgg attcattgca atggcgcgtg caatggctac acgttttttc atgccaccac
      301 tgatggcact tggcaaaaga tgattaacgt tttcgaggtt tactctatgc agacaaaagt
      361 tggcacgatc gcgcttttct gcattggtca tgttagtaaa cattttcaaa gggaaagtta
      421 cattttgttc aacggttaat gaatcaaaca atgcaccacc ctgaaacaac atgccaattt
      481 cctgacgaat gggtttacgt tcgccaaaag tcatctccga aaaatttctg ccatcgtata
      541 aaactttccc cttatcaact tcaaataaac caacagcaca tttcatcagc acagtttttc
      601 cttgtccact accgccaata atcaggttaa ttttaccttt gtcaaattcg catgaaacat
      661 cttcaagcac ttttttattg ccgaatgatt tggatatgtt ttcaatggca atcatgcaag
      721 aatgagttgg gttaataagt aatcaaaaat cagaattaaa atactgctgt aaactacacc
      781 tttggtactt gactggccaa cttcaagtgc gccaccgctt gtataatagc catgataggc
      841 aggaacagaa gtgataataa aagcaaacac tactgtcttt atcatagcaa aggttacatt
      901 gaatggtaag aactggtttt gtattccttt taaataatct gccgaaggca caacgcccga
      961 aagatcacct gcaacccatc cgcctgtaat accaacaatc atactgatga ttacaactac
     1021 gggacaaatt aataatgctg caataatttt aggaagcacc aaataccctg atgagtttat
     1081 gcccattatc tcaagtgcat ctatctgttc ggtaacacgc atggtgccaa tttctgatgc
     1141 aacactgcta cctacttttc ctgcaagtac aagcattatg atagttggag aaaactcaat
     1201 gatgatacta tcgcgggcaa caatacctac tgcccacaaa ggcaccattg ggttcacaag
     1261 gttgtaagca ctttgaattg ttataacagc acccatgaaa gcagaagtaa gtgccaccag
     1321 ccacaatgaa ccaatgccta agtttaccat ttcgcgaaaa acctggcgta cataaattga
     1381 caacttttca ggtttataaa aaagattttt tataaaaccg aaatagcgtc caatctcaaa
     1441 taatgtacct ttcatcagta gctaagatat taaaactatc tattttactt cttttaaaaa
     1501 tacgagatag gttggaaagt gatcgctgta gccgccaata tatctgctga agtcgaatgt
     1561 gcgtaaggga tagcctttat aacgtccgct cttttgcaac ataaattcgc gtttaaaaat
     1621 atgggcatct ttaaaaaaga atccatcttg ctttttgttt aaccagcctg atgaaatcat
     1681 aatctgatca aaaagattcc atgcatcgtt ataggcaagt gtacctattc cttttgtata
     1741 aaaatcaacc catggattgt acatgcctcc gcgttctact ttgtctttac tgcccactgc
     1801 cttaattact tttgtgacac ttggacttaa agggtcatca tttaaatcgc ccatcagcac
     1861 aatctttgca tcggcattta ttttagttat agaatcaatt ttgtttttac aaacagcggc
     1921 agccttagcg cgtgatggtg cagaagcctc ttcgccccct ctgcgcgatg gccagtgacc
     1981 aacaagaaaa tgtacaggct ctccaagata caaaccataa acatataata tgtcgcgggt
     2041 gtagtattcg gggttttcag gagtacgcaa gtcaatgaat aaaggctcac taaacttagg
     2101 agtaaaatat tttggattat agagcaatgc tacatcaaca ccgcgtttgt cagggctgtc
     2161 gtaatgaaca attttcagat tgcgtgattt tagtttgggt tcattaacca aatcttttaa
     2221 aacactttca ttctctactt ctgcacatcc caaaacagaa aatccatctg gtgactcgtc
     2281 ttttccaatc agtgataata catcagagag tttacctaac ttatcaagat atactttgcc
     2341 tgtgtaattg ttagaacctg tcggagtaaa ctcttcatca ttaacttcgg gttgattgat
     2401 ggtatcgaac aggttttcta aattgtagaa agcaataaca cctacctgat agcttttctt
     2461 ttgttgtgcc tgaaggcctg tagccatgga aattatccag gctgtaataa gtatcttttt
     2521 taacatggca caaatctaaa aaacgtcaca atgctgtggc ttgaaattat gatgattaaa
     2581 tttaattttt aaaaagatta tcttcttctt ttgccaccct tctttttatc gcccctgaac
     2641 ttgcctgatg tagggaatct ttttttatca cttcgtttaa aaggagaaac ttcatgcaaa
     2701 cgatttgcat cataaagcat aaaatcaact tgttttttaa tgatgtcggt actcttaatg
     2761 acaaccatca gctcatcgcc caaagaatat tttttgcggg aacgatgtcc tacaatgcaa
     2821 aagtttttct gatcaaattc ataatagtca tcattcaggt cgcgcagcct tatcatacct
     2881 tcacacttat tatcctccaa ctccacataa agaccccatt ccgtaacacc ggaaattaca
     2941 cctttaaata tttcatcttt acgtgctgca aggtattgaa cctgtttgta ttttattgaa
     3001 gagcgctctg catcggcagc attaatttca cgctccgtac agtgacggca tttttcttca
     3061 tactcttgtt gcgaaacact ctttccacca ctcatataat aatccagcaa tcggtgcacc
     3121 atcacatcag gatagcgcct tatgggcgaa gtaaagtgtg tatagaaatc gaatgccaat
     3181 ccatagtgac ctatattttc ggtagaataa acagccttag ccatactgcg tatagccaat
     3241 tgctcaatca tactctgctc ttttttacct tccacatctt tcagcatttt attgagtgaa
     3301 tgagctgttt cttttcctga tgcggtgcta atcctatagc caaactgtgc tgcaaaagtt
     3361 ttaaagtcgc taagtttttc ttcaacaggc acatcgtgga tacgataaac aaaagttttt
     3421 tgtgttttat ctttcttccc ttttgcaaca cctttgccaa taaactctgc cacacgtctg
     3481 ttggcaagta acataaactc ttcaatcagt ttattggagt ctttgctctc ctttagaaat
     3541 attccaattg gatttccctt ttcatcaagc ctgaatttta cttctgtttt ttcaaaactg
     3601 attgctccct tacggtgacg ctctttgcga agttgctgtg ccagccggtg aaacacacca
     3661 atctctttta caaaagggcc ttcgttggtt tcgattacct gctgtgcttc ttcataagta
     3721 aagcgatgat ctgaaaaaat tacagttctg ccgaaccatt cattcaggac ttctgctttt
     3781 tcattcatct caaaaacggc agagtagcaa agtttttcct cattaggtct tagagagcaa
     3841 accatattcg acagtttttc cggcaacata ggaataactc tgtcaaccaa ataaattgaa
     3901 gttgctctgt tgaaggcttc ttcatccatg gcactaccca gttttaagaa atgtgaaaca
     3961 tcggcaatgt gtacacccat ttcccagttg ccattaggca actgctgaat gcttaatgca
     4021 tcatcaaaat cttttgcatc aaccgggtca atagtaaagg tggttacttt tctgaagtct
     4081 ctgcgttttt taatttcttc tttcgaaatg acatcagaaa tttttaatgt ttctttttca
     4141 acttcatcag gaaaggcttc cggaagacca tactcaacaa ttatggcatt catctctgca
     4201 gtatgttcac cgggagcacc taaaacacgc actatttttc cttcgggatt actggcatcg
     4261 gcagaccata aggttatctc tgcaattact ttatcgccat tgcgggcacc attaagttgt
     4321 tttccgggaa taaaaatatc tacatgcatt ttaggatcat cgcaaactac aaatgcataa
     4381 ctgcctgaat gttgtattgt gccggcaaat tgtgttctgc ttcgactgag aacttcaacc
     4441 acttctcctt gttgtcttct gccttcgcga tgcgctaata aacttattct gactttgtcg
     4501 ccattcaatg cgtttgccat taaatgtgca ggaatttgta tatcctcttc aaacccttca
     4561 ttcatcacat atccttcgcc tttggaagtt acctgaatgt ttccttcaat aaacctttct
     4621 gcaggtaata acttataacg aaactgctga acctccagca actctccttc ttgaaacagc
     4681 tctaaaatta aagattgaat tgtttcgtct atataatgtt tatcagcatt cagcactact
     4741 gaaatatatt cttcgggcaa atttatttct gctttgcgcc aaacctgttt gggattaaaa
     4801 gtagcacgcg ggtgttgcag aaaaacctgt agcaattctt gttttaattt ttcaagttgt
     4861 gtttttgtga attttatatt cttctttgac atgttttcgt ttgattgtaa aatt
//
LOCUS       DAOOOL010000164         4896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1889_length_4896_cov_139.969015, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000164 DAOOOL010000000
VERSION     DAOOOL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_4896_cov_139.969015"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKU82_10675"
     CDS             complement(<1..679)
                     /locus_tag="PKU82_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOZ83378.1"
                     /translation="MIAVLLAGGKGTRLAPFTISLPKPLMPVGNRPILEILIKQLKDA
                     GVTKVVICVGYLESLIRSYFGDGSKFGVEIIYSSETEPLGTAGPMKLIESYLTDSFFF
                     MNGDLFTDINLEEMFRFHKDNKAVATIGLTRRTVDIDFGVIKIDQTNEFTEWKEKPTL
                     EYLVSMGIYVFEPSVLKYLPDGFFNVPDLIIKLHEAKESVKGYQYKGNWLDIGRLEDY
                     QRACQQME"
     gene            complement(689..1663)
                     /locus_tag="PKU82_10680"
     CDS             complement(689..1663)
                     /locus_tag="PKU82_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOZ83379.1"
                     /translation="MKNVLITGAGGFIGSHLTEQCVEAGYNVKAFVRYNSKSNYGWLE
                     GSKYASQIEFITGDIRDYDSVHHAVKGCDTVFHLAALIGIPYSYVSPMAYIKTNVEGT
                     YNIVQSCREHGTENVLVTSTSETYGTAQYIPIDEKHPLVGQSPYSASKIGADQIALSY
                     FLSFNTPVKIVRPFNTYGPRQSARAVIPTIITQLLSGKSDIKLGHITPTRDLTFVKDT
                     ANGFITIAQSSKFTGSVTNIGMNNEISVGDLAKLIAEMMGKKINIITDAQRVRPDNSE
                     VERLYCDNTKLITNSNWKPAYNIQSGLKETIDFLEKHLHLYKPDIYNV"
     gene            complement(1675..2997)
                     /locus_tag="PKU82_10685"
     CDS             complement(1675..2997)
                     /locus_tag="PKU82_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83380.1"
                     /translation="MLDFYLTIASYLGLAVIVIVGLFFNRHFFLTLFIVKPFIDMTVN
                     VSVIGDFNALEVSGVFSALILLIKYLNKPIAYPTFNEIWIWIFIGLQLLAFFLNVSAD
                     TRSIFISTKVYLKMLNSYLVYFLAAIEIFENFKQRVNLVKIVWLTALFAGLITILVFA
                     LGLSNFDTTKGVVRYNGLYNDPGTPSYLSVICLLFCNLYFKIAQKKPNHFFQFLRILT
                     LAVTAVILVITMTKSALLMFLVYVIMWYGIYERHLFLIVPALLISIYFSFSLSDDLNT
                     RFETEINYVDSGGDAEAAKSMGTGRVNRWETLLDVYFNELGPMNQLFGTSKHWMAHNQ
                     YIAYLMQVGIVGLTVFLIFLIRFIVKLYSIYHRTRNPSVYAAFTLLIMYVFYAFTGHP
                     FDYTTLLWYLMILLSMINVYEVHQMRNKQQKLKAITDKNFLLNKTQLN"
     gene            complement(2990..4135)
                     /locus_tag="PKU82_10690"
     CDS             complement(2990..4135)
                     /locus_tag="PKU82_10690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ83381.1"
                     /translation="MREVKKVLIVTNVPQDYRIAMFNALNLLLQKENIQFKVAFGAAG
                     YKRRKSKLDFNKMKFDYTILESTKFTFGSTENTMFTYPGLLKLHKEYRPDMTFVIGYS
                     PATIKLWFYSFFMNINYFIWSGSVNFKGRKDSTLRKILRKILVKRAKGFIAYGTKAKE
                     YLVYLGAPENKIHIAINTVDTHFFNEIAKKRLNRKVNTQKQLLYLGYLTPGKKIDDLI
                     KTINELKNLRNDFVLRIIGDGPAIENLKSLTSELHLTDIIKFEGFKQKNDLPPFMEQT
                     DCFLFQTGCDIWGLVLNEAMAAGLPCIVSPNAGAARDLVFDTKNGLILDFGSHKIAAS
                     RINELLNDEVLLQKMGYSANEFIESNVTAEKSAQGFISAIKQYCSHA"
     gene            complement(4128..>4896)
                     /locus_tag="PKU82_10695"
     CDS             complement(4128..>4896)
                     /locus_tag="PKU82_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOZ83382.1"
                     /translation="NHLKYIHNYKTFDKFSKLPTSIKKLVSGTFSLINSNSPLSEMLS
                     RAAQNQEFYWSSARSFKESVKSRFLHPDFRNRHQSLNSYSIIKAFKEEYNAIKKPFHD
                     HIDWMCFMGFKLTDPHWYLYRGDKLGMAHSIELRSPFLDYEFVNFALSIPGSMKVKNN
                     EPKYILKKSLEAILPDEVLYRKKMGFCLPLKQWGGEIMIDYVESNYVEFCKNFNLLNQ
                     DGIKQILNNVKGGNERSVNDLWTAYFLISWFKRWMHA"
BASE COUNT         1610 a          859 c          793 g         1634 t
ORIGIN      
        1 cttccatctg ttggcaggca cgttggtaat cttccaagcg gccaatgtct aaccaatttc
       61 ctttatattg atatcccttt acactttcct ttgcctcatg cagttttata atcaaatcag
      121 gaacgttaaa gaagccatca ggcaaatatt ttaacacgct aggttcaaaa acatagatcc
      181 ccatacttac taaatattca agagtgggtt tttctttcca ttcagtaaat tcattagtct
      241 gatcaatttt aattactcca aaatcaatat caactgttct gcgagtaaga ccaatagtag
      301 caacggcttt attgtcttta tgaaatctga acatttcttc aagattaatg tcggtaaaca
      361 aatcaccatt catgaaaaag aatgagtctg ttaaatatga ttcaataagt ttcattggac
      421 ctgctgtacc taaaggttct gtttcacttg agtagattat ttcaacgcca aactttgagc
      481 catcaccaaa ataagaacgt attaatgatt caaggtaacc aacacaaata acaaccttag
      541 tcacacctgc atctttaagt tgtttgatta atatttctaa aataggtctg ttgccaacag
      601 gcattaaagg ctttggtaaa ctaatagtaa aaggcgctaa gcgtgtgcct tttcctcctg
      661 ctaataaaac tgctatcata tcttgaattt aaacgttgta aatgtcaggt ttgtaaagat
      721 gcaaatgctt ttcgagaaag tcaatagttt cttttaagcc tgattgaata ttgtatgccg
      781 gtttccagtt actattggta atgagtttgg tattgtcgca gtaaagacgc tctacttcac
      841 tgttatcagg acgaacacgc tgagcatcgg taataatatt tattttcttt cccatcattt
      901 ctgcaattag tttagccaaa tcaccaacac tgatttcatt gttcatgcca atattagtaa
      961 cagatccggt aaatttggat gattgggcta ttgtgataaa tccgtttgca gtgtctttaa
     1021 caaatgtaag gtctcttgta ggagtgatgt gccctaactt aatatcactt ttaccactta
     1081 ataattgcgt gatgattgta ggaattacag ctcttgcact ttgacgtggg ccataagtat
     1141 taaagggtct tacaattttt acaggcgtgt tgaatgaaag aaagtaactt aatgcaattt
     1201 ggtcggcacc aatttttgaa gcagagtagg gtgactgtcc aaccaatggg tgtttttcat
     1261 caattggaat atattgtgca gtgccataag tttcactcgt agatgttaca agaacatttt
     1321 ctgtgccatg ttcacggcaa gattgaacaa tattatacgt gccctcaaca tttgttttta
     1381 tataggccat tggagaaaca tagctgtaag gaatgccaat taatgctgct aagtgaaaga
     1441 cagtatcgca gcctttaaca gcatgatgta cactgtcata gtctctgata tcgccagtta
     1501 taaattcaat ttgactggca tatttagagc cttcaagcca gccataattt gactttgaat
     1561 tatatctgac aaatgccttg acattatatc cggcttcaac acattgttct gtgagatgtg
     1621 aaccgataaa tcctcctgca cctgtaatga gtacattttt catctgatat tttattaatt
     1681 tagttgtgtt ttgttaagta agaaattttt atctgttatc gctttcagtt tttgttgttt
     1741 gtttcgcatc tgatgtactt catatacatt aatcatagaa agtagaatca ttaaatacca
     1801 aagtaatgta gtataatcaa atggatgacc agtaaaggca taaaatacat acatgattaa
     1861 aagtgtaaat gcagcataaa cagaaggatt gcgtgtgcgg tggtaaatac tgtatagttt
     1921 tactataaac cgtattagaa atattagaaa gactgttaaa ccaacaatgc cgacctgcat
     1981 taaataggcg atatactgat tgtgtgccat ccaatgtttg gatgttccga aaagttggtt
     2041 cattggcccc aattcattaa aataaacatc aagtaaagtt tcccaacggt taactctacc
     2101 tgtgcccatt gatttggctg cttcggcatc tccacctgaa tccacatagt taatttctgt
     2161 ttcaaatcgg gtattcaggt catcagataa gctgaacgaa aaatatatag aaattagcag
     2221 cgcaggaaca attaaaaata aatgcctttc gtagattccg taccacatta taacgtaaac
     2281 taaaaacatg agtaaagcac ttttagtcat tgtaataaca agaataactg cggtgactgc
     2341 aagagttagt attcttagga attgaaaaaa atgattgggt ttcttttgtg caattttaaa
     2401 atacaaattg caaaataata agcaaattac agaaagatag gaaggggttc caggatcatt
     2461 atacagtcca ttatatctta ctacaccttt cgtagtatca aagtttgata atcctaaagc
     2521 aaaaaccaga attgtaatca atcccgcaaa tagagcagtt aaccaaacta tttttactaa
     2581 atttactcgt tgcttaaagt tctcaaatat ctcaattgct gctaagaagt aaaccagata
     2641 ggagttcagc atcttcagat atacttttgt acttataaag atggagcgtg tatcggcaga
     2701 tacattcaaa aagaaagcaa gcaactgcaa gccaataaaa atccaaatcc aaatttcatt
     2761 aaatgtagga taggcaatag gcttatttaa gtacttaatt aataaaatta aagcactaaa
     2821 cactccggaa acttccaatg cattaaagtc tccaattaca ctgacattaa ctgtcatgtc
     2881 tataaatggt ttgactatga atagtgttaa aaagaaatga cgattgaaga aaagcccgac
     2941 aatcacaata acggccaagc ctaaataaga agcaatggtt aaataaaaat caagcatggc
     3001 tacaatattg ttttattgct gaaataaatc cttgagcact tttctctgct gtgacgttac
     3061 tttctataaa ctcattagcg ctatatccca ttttttgaag aagcacttca tcgtttaaca
     3121 gttcgtttat tcgacttgct gcaatcttat gtgatccaaa atctaaaatc agtccattct
     3181 tagtgtcaaa aactaaatca cgagcagcac ctgcattagg cgaaactata caaggtagtc
     3241 ctgctgccat tgcttcattt aaaaccaaac cccaaatgtc acagcctgtt tgaaataaaa
     3301 aacaatctgt ctgttccata aacgggggca agtcgttttt ttgtttgaaa ccctcaaatt
     3361 taatgatgtc tgttaaatgc aattcagaag ttagtgattt taaattttca attgcaggtc
     3421 catcacctat gatacgtaaa acaaaatcat tgcgaagatt ttttaattca ttaatcgtct
     3481 taatgaggtc atctattttt tttccgggcg ttaaataacc taaatacaat agttgttttt
     3541 gagtattaac ttttctgttt aagcgtttct ttgcaatctc attaaaaaaa tgtgtgtcaa
     3601 cggtgtttat tgctatatgt attttgtttt caggtgcgcc taaataaact aaatattctt
     3661 ttgctttggt gccataagca ataaaccctt ttgctctctt aactaatatt tttcttagta
     3721 tttttctaag agttgaatct tttcgacctt taaaattaac cgagcctgac catataaagt
     3781 agtttatatt cataaaaaac gaataaaacc aaagtttaat ggttgcaggg ctatacccga
     3841 taacaaatgt catgtccggt ctatactctt tatgtaattt taaaagtccg ggataagtga
     3901 acatggtatt ctctgtagaa ccaaatgtaa acttagttga ctcaagaatt gtgtaatcga
     3961 atttcatttt attgaaatcg agtttggatt ttctccgttt atatccggct gcaccgaatg
     4021 caactttaaa ttgtatattc tctttctgca gtaataagtt taatgcattg aacatagcaa
     4081 ttctataatc ctgtggcaca ttggtaacta taagaacttt ttttacctca cgcatgcatc
     4141 catcttttaa accatgatat taaaaaatat gctgtccata aatcatttac agatctttca
     4201 ttgcctcctt ttacattatt caatatttgt ttgattccgt cctgatttag taaattaaaa
     4261 tttttgcaaa attctacata gtttgattct acataatcaa tcatgatttc accaccccac
     4321 tgctttaaag gtaaacaaaa gcccatcttc tttctgtaaa gaacctcgtc aggcaaaatg
     4381 gcttcgagtg acttttttag aatatatttc ggctcattat ttttaacttt catacttcct
     4441 ggaatagaaa gagcgaagtt aacaaattca tagtcaagaa atggagaacg aagttctata
     4501 gaatgagcca tacctaactt gtcaccacga taaagatacc aatgagggtc agttagttta
     4561 aaacccatga aacacatcca atcaatatga tcgtgaaaag gttttttgat agcattatac
     4621 tcctctttaa aagctttaat gattgaatag gaatttagtg actgatgtct gttcctgaaa
     4681 tcagggtgca gaaacctact ttttactgat tctttaaaac ttctggcact actccaataa
     4741 aattcctgat tttgagctgc acgacttaac atttcgctta atggagaatt cgaatttatc
     4801 aatgaaaaag ttccggaaac aagcttttta atgcttgtag gtaacttact gaacttatca
     4861 aaagttttgt agttgtgaat atattttaaa tggtta
//
LOCUS       DAOOOL010000165         4818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1927_length_4818_cov_107.947722, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000165 DAOOOL010000000
VERSION     DAOOOL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_4818_cov_107.947722"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKU82_10700"
     CDS             <1..495
                     /locus_tag="PKU82_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83383.1"
                     /translation="KNIQDTLKSKKQVQFTTPSTKLSDKNVKTEESVFHYYQSTPKRV
                     SVEITPWHDGKRELNFFDQFGNKTYSINDIRRSYSSTTELKEFHKNGACSLVKTSVNP
                     DASMYWYETEIIFDENNNPQWKTELQFPQESTEQNLNNKWWWNTRTNSWVKQEIIREQ
                     PVPH"
     gene            629..2602
                     /locus_tag="PKU82_10705"
     CDS             629..2602
                     /locus_tag="PKU82_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83384.1"
                     /translation="MINRIIYITIFLLISVNQNILSQSNSARLFPQEKINEALNKFVN
                     SAQWSKQNKQSSQSPTGFVSSPSSVLFKKVFDNNEQALMAADTIIVGAQVNDTLVITG
                     NFTHTGPILVFNSGVLIFNQATVIDTGEIYVLGNGKLFADSTTFTFPQQYIYERSLIV
                     AQNGFVQVKNSSFNYSGMSHNLYLADSAKVELTNIHQNDFTTCGLYGNANININGCNI
                     TGEYILTDQCQATFKNADTLILWHHFPDAAVVNYSFPPGDTVYNYICNNNTPGINGIG
                     YQVSADSCHNVMWALMPVNGSDVTISNSNLRLIGCWFERGDLATVSGIFNNSNYSNYT
                     APLSDRNLHLINTDVQTWSFYVFDSSQVDIDSCQLGEVGTQQSALVQANNLLLDGSGG
                     YFWATDSSGILALGTTIYTMARSEKKGIFILAYGWQPFMAPTALSNSLMVCVQSTLGQ
                     DPVPYDGAVAWMQKISSPDTAIINTVVPINGSAWIDQGPSGGGMDFDKYSLYWSASTS
                     NWIPIVTNVITEVHNNGLLGSWNTNGFTAGNYFLRLMIKNNLGDSLEAIKPVYLKQLV
                     SVNELSSIQSLIIYPNPAHDKISIEMSDIPESSEYNVEVLSATGSEVYTSTFANSTNH
                     FNLDVSNLQQGLYYVLIKTNNGFLKKSKFVILK"
     gene            2840..>4818
                     /locus_tag="PKU82_10710"
     CDS             2840..>4818
                     /locus_tag="PKU82_10710"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83385.1"
                     /translation="MIRAGIAIWAVMLCMLTANAQNVVTLAGQSGISGAVNGSGTSAT
                     FNNPHGMVCDVAGNLFVADRNNHLIRKITPAGVVSTFAGSGSAGSSDGIGTAASFFEP
                     WGIAIDQSGNLYVADTKNYLIRKITPTAVVTTVAGAGTSGVTDGPASNAQFGFPTGIA
                     VDASGNIYVSEFMTHVIRKISNGSVTTFAGTAFQSGDANGTGINAKFDHPHSIAIGQS
                     GNLFVTDTWNNKIRKITPAGVVSTYAGSTAGYVDGAASVSKFDNPLGICADASGDIFV
                     GDVNNFCIRKISNGTVNTFAGTAGTAGAANGPLLQASFNSPSCMAYHSSDKYFIGDEG
                     NELIRLINMNQSSNPLTLSTLNNVSVFCPSSTSVITASPQGLSSYTFKEGSTVLATNT
                     TGILSLGTLSAGTHLISCTATGLLGNYATTTALSITIVDASTPVISPSGPISICSGGS
                     ATLTASPGIAYLWTNGATTSSINATLPGTYAVSITYTGGCSYQSNSVTVNLNGSFSAS
                     ISPSAAQVICPGDSVKLTATAGTSYLWSNGKTTQSIYAKTAANYTVAVTNSFGCTSVS
                     PPVNVSLKSMPATIDASSDSSCSGEGVLLTVVQQNNTTYSWFDQATGGTALATGSSYA
                     TAPLAESTTFYVQLNSNGCTSISRFPVLAYIYPAL"
BASE COUNT         1603 a          850 c          919 g         1446 t
ORIGIN      
        1 aagaatatac aagatacatt aaaatcgaag aagcaagtac aatttactac gccttcaaca
       61 aaactatcag ataaaaacgt aaagaccgaa gaatctgttt tccattatta tcaatcaaca
      121 cctaaaagag tatcagtaga aattacaccc tggcatgatg gtaaacgcga actgaatttt
      181 ttcgaccaat ttggaaataa gacttattcg ataaacgata taagaagaag ttattcttcc
      241 acaacagaac tcaaagagtt tcataaaaat ggcgcatgtt ctctggtaaa aacttcggtt
      301 aatcctgatg caagtatgta ttggtacgaa acagaaataa tttttgatga aaacaataac
      361 ccgcaatgga aaacagaatt acaatttcca caagagtcaa cagaacaaaa cctcaataac
      421 aaatggtggt ggaatacaag aacaaatagt tgggtgaaac aggaaataat tagggaacag
      481 cctgtgccgc attaatcttg gttttatttt agttttcttg aattcaaatg atttctgtta
      541 ttatttttac atttgataaa tagcaggaac aatcatgctt agttttatta caaagctcaa
      601 gttgagatat gtttaaaaac atcaaccaat gataaatcgc ataatttaca ttacaatatt
      661 tttgttgatt tctgtaaatc agaatattct ttctcagagt aattcagcaa gattatttcc
      721 gcaggaaaaa ataaatgaag cattaaataa atttgttaac tcagcacaat ggagcaagca
      781 gaacaagcag tcaagtcaat caccaaccgg ttttgtttca tcaccctcat ctgtactttt
      841 caaaaaagtg tttgataata atgaacaagc ccttatggca gctgatacca taattgtagg
      901 tgcacaggta aacgacacat tagtgattac aggaaacttt acacacacag gccccatttt
      961 agtttttaat agcggtgttt taatttttaa tcaggcaaca gttattgata caggtgaaat
     1021 ttatgttctt ggtaatggaa aattgtttgc tgactccaca acatttactt ttccacaaca
     1081 atatatttat gagcgaagtc ttattgtagc acaaaatggt tttgttcaag taaagaattc
     1141 atcatttaac tatagcggta tgtcacacaa cttatattta gcagactccg caaaagtgga
     1201 acttaccaac attcatcaaa acgattttac aacatgcggt ttatatggta atgctaacat
     1261 aaacatcaat ggctgtaaca taaccggtga atatatttta accgatcaat gccaggctac
     1321 atttaagaat gcagatactt taattctttg gcatcatttt ccggatgctg cagtagtaaa
     1381 ttattctttc cctcccggtg atacggttta caattatata tgcaacaaca acactcccgg
     1441 aataaatgga ataggatatc aggttagtgc agacagttgt cataatgtaa tgtgggcttt
     1501 aatgcctgtt aatggttctg atgttactat atcaaattca aacttacgat taattggatg
     1561 ttggtttgaa cgaggtgatt tagccacagt ttcgggcatt tttaataatt caaattatag
     1621 caattatact gcaccattat cggacagaaa tttacattta attaatacag atgtacagac
     1681 atggagtttt tatgtttttg attcatcaca ggttgatata gacagttgtc agttaggtga
     1741 agtgggcaca cagcaaagtg cacttgtgca ggcaaacaat ttattacttg atggaagtgg
     1801 cggatatttt tgggcaacag acagcagtgg cattcttgct ttaggcacaa ctatttatac
     1861 tatggcaaga agtgagaaga aaggaatttt tattcttgct tatggctggc aaccttttat
     1921 ggcaccaact gcattaagca acagtttgat ggtttgtgtt caaagcactt taggacaaga
     1981 ccctgttccg tatgatggag cagttgcatg gatgcaaaag atttcatcac ctgatacagc
     2041 tataatcaat actgttgttc cgattaatgg atcggcttgg attgaccaag gaccatcagg
     2101 tggaggaatg gactttgata aatacagttt gtattggagt gccagtactt caaattggat
     2161 accaattgtt acaaatgtaa tcactgaagt tcacaacaat ggattactcg gaagttggaa
     2221 taccaatgga tttactgcgg gaaattattt tttgcgtttg atgataaaga ataatttggg
     2281 tgacagcctt gaagcaatta aacctgttta cttaaagcaa ttagtttcag ttaacgaact
     2341 atcatctata caatcattaa ttatttatcc taacccagcg catgacaaaa ttagtattga
     2401 aatgagtgac attccagaga gtagtgaata caatgttgaa gtgttaagtg caaccggtag
     2461 tgaagtttat acaagcacat ttgccaattc aaccaatcat tttaatcttg atgttagcaa
     2521 tttacaacaa ggattatatt atgtccttat caaaacaaat aacggttttt taaaaaaatc
     2581 gaagtttgtt attttgaaat agtatgaatt gattttattt ttcgatcatt ttatatacag
     2641 attcatcaaa atacatttca cataagtata aagttacatt gacaatttag tcaaacagtt
     2701 gatcttgtcc gtaaattgtt gtttcagagt tcctaatcac attttcaaag agtaattttt
     2761 ttctgatgta acgcttcaat ggtgtaacct tactttaaat agaaagtata aaagggagtt
     2821 cacttttgaa gaaaaacgaa tgataagagc cggaatagca atatgggctg tcatgttatg
     2881 catgttaaca gccaatgctc agaatgtagt tacactggca gggcagagtg gcatttcagg
     2941 tgctgtcaat ggctcgggta cttctgcaac gttcaacaat ccacatggaa tggtttgcga
     3001 tgtggcagga aacctatttg ttgcagacag aaataatcac cttatcagaa aaataacacc
     3061 tgcaggagta gtatcaacat ttgcaggatc tggtagtgca ggaagttcag atgggatagg
     3121 aacagcagca tcattttttg aaccttgggg aattgccatt gatcagtcgg gaaaccttta
     3181 tgttgccgac acaaaaaatt atttaataag aaaaattacg ccaacagcag ttgttacaac
     3241 agttgccgga gcgggaactt caggcgttac tgacgggccg gcaagcaatg cacagtttgg
     3301 atttccaaca ggtattgcag tagatgcttc aggaaatatt tatgtgagtg agtttatgac
     3361 acatgttatc cgcaagataa gtaacggtag tgttaccact tttgcaggaa cagcttttca
     3421 gagtggcgat gccaatggta ccggaattaa tgcaaagttt gatcatcctc atagtattgc
     3481 tattgggcag tcaggaaatt tatttgttac agatacctgg aataataaaa tcagaaaaat
     3541 tactcctgca ggtgtagtga gcacctatgc aggaagtact gcaggatatg ttgatggtgc
     3601 cgcttctgtg agtaaatttg ataacccttt gggcatttgc gcagatgcat caggcgatat
     3661 atttgttggt gatgtaaata atttctgtat ccgtaaaatc agcaatggca cagtaaacac
     3721 ttttgcagga acagcaggaa cagcaggcgc ggctaatggc cctttgcttc aggcctcatt
     3781 caattcacca tcatgtatgg catatcattc atcagacaaa tattttattg gtgatgaagg
     3841 aaatgaattg atccgactca tcaacatgaa tcagagtagt aatccactaa cactttcaac
     3901 attaaataat gtgagtgtat tttgtccaag ttcaacttct gttattacag cttccccaca
     3961 aggactaagc agctatactt ttaaagaagg aagtacagta ttggcaacaa atacaacagg
     4021 gatattgtct ttaggaacat tatcagcagg tacacatctt atcagttgta ctgcaactgg
     4081 actattagga aactatgcaa caacaacagc attaagtatt acaattgttg atgcaagcac
     4141 acctgtaatt tctccttcgg ggccaatatc aatttgctca ggtggaagtg ctacattgac
     4201 agcttcaccc ggtatagcat acctgtggac aaatggagca acaacatctt caatcaatgc
     4261 caccctgcca ggcacttacg cagtgagcat aacttataca ggtggctgca gttatcaaag
     4321 taattcagtt actgtaaact taaacggatc attcagtgca tctatatctc catcagcagc
     4381 acaggttatt tgccccggtg actcggtaaa attaacagct actgcaggaa catcatactt
     4441 gtggtctaat ggaaaaacaa cacaaagcat ttatgctaag acagccgcaa actacaccgt
     4501 agctgtgaca aacagttttg gatgtacttc tgtttctcca ccagtaaatg tttcgttgaa
     4561 atctatgccg gcaacaattg acgcaagcag cgattcatca tgcagcggtg aaggtgtttt
     4621 gttaacagta gttcagcaaa acaacacaac ctatagttgg ttcgatcagg caacaggagg
     4681 cacagcactt gcaacaggca gcagctatgc tactgcacct cttgcagaaa gcaccacatt
     4741 ttatgttcaa ctaaattcta atggctgcac aagcatttca agatttcctg tacttgcata
     4801 catctatcct gctttaca
//
LOCUS       DAOOOL010000166         4787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1948_length_4787_cov_46.941674, whole genome shotgun sequence.
ACCESSION   DAOOOL010000166 DAOOOL010000000
VERSION     DAOOOL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_4787_cov_46.941674"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKU82_10715"
     CDS             complement(<1..557)
                     /locus_tag="PKU82_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83386.1"
                     /translation="MSTNVVWQTKSPVVLESFKQKKITSIIKGGKVYSFQAAMALAMK
                     YNVIIDENAIKHDNQTILKYFLKHRANTPKADVTVKEIYPVVVGAVNKKSKNVALIHH
                     IDQVHGGQSFGHKVFFMELFRKLLKMDLVITVSEFWKDYLEKKGCKNVKVIYNSFIPE
                     DYMIQKEAVAEFRKKYSIPDKPIVY"
     gene            complement(670..1641)
                     /locus_tag="PKU82_10720"
     CDS             complement(670..1641)
                     /locus_tag="PKU82_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83387.1"
                     /translation="MKYKLIDKPHFVSREVYTEVTNKLIDKLRPNKSIVSVYTIGHVG
                     NPGISDIDMLIIAKDNCLLSENYRAEMSLQEKYLFLHQPIACSESGFSKAQIFSFFHN
                     YNLVHGKDIRVNSDLNANDINILKKQTALEFLLKMYMQLYIQKKYKILRVRDLLLHGK
                     ALIYDLEFLNITSGNLFSLVQTVIEWRNKWFIETPADHEIIEWFENLLSELHLQFTKN
                     SLFREIAFPIAKTYSIAKNIKLNSGGNSFNVKHSGFNFPLASSLSSKKGVKLLNKFNN
                     FKFEVPSDIKSLPTILSERFSVEGQLRSYGKEFLPNFMPMTSSLHLN"
     gene            complement(1622..2647)
                     /locus_tag="PKU82_10725"
     CDS             complement(1622..2647)
                     /locus_tag="PKU82_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83388.1"
                     /translation="MTTKNSKHFEVVRKYFKNGCLNQSDVLLSLIHYRLPYLKNAGSI
                     LKLYNSYSTEVSSCSKSILVSNYQINYSHALAMKQYLNEKLNKFILGAFLHGSLGTNE
                     LVHYSDFDALVILNHSVFQSKQTLNKAAIALKKSEKIMQKMDPLQHHGWFVITEKELD
                     NFPINYFPPSLFEHAKCMIGENTFQIKYNNLSALNQLQSFNSYCSHLLNNIKRIEQNL
                     NYYEVKSLLSGFMLLPSIYLQLKNPEGIFKKESFSLINKSLGDKEYEVMNQVSDIRAK
                     WDYQPSLLYKILLSLNLTSLNYFYKKKASGRIPVSLKNSLIDMRPAMTKFIQTLLSKA
                     KHEVQTD"
     gene            complement(2656..3804)
                     /locus_tag="PKU82_10730"
     CDS             complement(2656..3804)
                     /locus_tag="PKU82_10730"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ83389.1"
                     /translation="MHKRILEKLICPNTNLQPLQLYALEIKRKQELLSNLDNTSILPN
                     DDIKTGILYTQDKRNLYFIYEFIPILLNDSDQDLIHIKEIHQKVRSLIPTEFIQAIEQ
                     NILRIENLNQSSDGNWNREEMKYYDAAVETAQLRDKMLQSIQNDNLWRIFIPRKEKMI
                     SHLEVNCNGQWIFEIGCGNARTVARMFNPATYKYNYIGTDISFKRLMVAKMAIPESDF
                     IQASALNIPLLNNTFQGAISFGMLHHLPRPAEAVTEVNKKLSQGALFTYHEPVYTSRQ
                     FSEKQSAMVRKIFRTYDHSDHDNKINLKESLAELKNVNHQIISIVPYNSVFRVVFEAI
                     INRFYKSWQKSKLVMKLVLVIDNVFLKTIVRLSNKLGPQAIIVVSKKL"
     gene            complement(3876..>4787)
                     /locus_tag="PKU82_10735"
     CDS             complement(3876..>4787)
                     /locus_tag="PKU82_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83390.1"
                     /translation="ALANALKKENQITIHTRNKQVISDINTQRKNISYFPNKILSKKI
                     KATSDITFANEADFLFLCIPSHAVEGYVKTLNLNEDCIIINGSKGFGTYNKLIPELLG
                     DLLPNEIASLKGPSFANELIFEIPTSFTIATAYEKDFNRIKELFRSELTVFDFTTDIR
                     GVEFLSILKNIYAIIVGITDAYYNSPNVRFLVFTKSLNEIKKITEVLAIPFDSLFNYA
                     GIGDFGLTALNDLSRNRTLGLLIGKGFFGKDLGNPIVLEGFKAIDIIIKMFDEQQIDQ
                     LKYIRLLQDLFNNKINVKDFVNKVIYT"
BASE COUNT         1543 a          720 c          741 g         1783 t
ORIGIN      
        1 atataaacta taggcttgtc aggaatagaa tatttttttc tgaattctgc gacagcttct
       61 ttttgtatca tgtaatcttc tggaataaaa gaattgtaaa tgactttaac atttttacac
      121 cctttttttt caagatagtc tttccaaaat tctgaaacag taataactaa atccattttc
      181 agaagcttcc tgaaaagttc cataaagaaa actttatgac cgaatgattg tccaccgtga
      241 acctgatcta tatgatgaat caaggctaca ttctttgatt tcttatttac tgcgccaact
      301 actaccggat aaatttcttt gacagtgaca tcagcctttg gtgtatttgc tctatgtttt
      361 aggaaatatt ttaatattgt ttgattatcg tgctttatgg cgttttcgtc aataataaca
      421 ttatacttca ttgctaaagc catggctgct tgaaaactat atactttacc acccttaatt
      481 attgaagtta tcttcttttg cttaaaagac tctaacacaa ctggcgattt ggtttgccaa
      541 acaacattag tggacatcaa aaaaaagtga aaattttaat gcaaatatta aggtcgcaaa
      601 tttacacttt taaaggattc attaagcctt tttaatagac tgtaaatgag tgctataaat
      661 gatatggaat tagtttaagt gcaggctgga ggtcataggc ataaaattgg gaagaaattc
      721 tttgccataa cttcgcaatt ggccctcaac agaaaatctt tcagaaagta ttgtaggtaa
      781 tgattttata tctgacggta cttcaaattt aaaattatta aacttattca aaagtttaac
      841 acctttcttt gatgagagcg aacttgctaa tggaaaatta aatccgctat gtttaacatt
      901 aaaagaatta cctccggaat ttaatttaat atttttagct attgagtaag tttttgcaat
      961 tggaaatgcg atttcacgaa acaaactatt ctttgtaaat tgtaaatgta attcacttag
     1021 caaattttca aaccattcaa ttatttcatg atcagcaggt gtctcaataa accatttatt
     1081 acgccattct attactgttt gaactaatga aaacaagttt ccggaagtaa tatttaaaaa
     1141 ttcaagatca taaattaaag ctttgccatg aagtaataaa tcacgaactc tcaatatttt
     1201 atattttttc tgtatataca gctgcatata catttttaga agaaattcca gtgctgtttg
     1261 tttctttaga atattaatat cattagcatt taaatctgaa ttgactcgaa tatctttacc
     1321 atgtacgaga ttgtaattat gaaagaaaga aaatatttga gctttactaa atccggactc
     1381 tgagcaggca ataggttgat gaagaaacag atatttttcc tgtaaagaca tttctgccct
     1441 gtaattttca gataataggc agttgtcctt agctataatc agcatatcta tgtcagaaat
     1501 tccgggattt ccaacatgtc caatagtgta cactgataca atacttttgt ttggtcttaa
     1561 tttatcaatg agtttattag ttacttctgt ataaacttca cgtgatacga aatgtggttt
     1621 gtcaatcagt ttgtacttca tgttttgctt ttgatagaag tgtttgaata aattttgtca
     1681 ttgcaggtct catgtcaatt agactgtttt tgagtgagac agggattctg cccgaagctt
     1741 tctttttgta gaaataattt aaggaagtta ggtttaaaga caataaaatt ttatataata
     1801 aagaaggttg ataatcccat ttagctctga tatctgaaac ctgattcatg acctcatatt
     1861 ctttgtcgcc taatgattta ttaattaagc taaaactttc ttttttaaaa atcccttctg
     1921 gattttttaa ttgtaaataa attgaaggta gaagcatgaa tccgctcaat aaacttttta
     1981 cttcgtaata gttaagattt tgttcaatcc ttttgatgtt gttcagtaaa tggctgcaat
     2041 atgaattgaa tgattgtaat tggtttaatg cggataaatt gttgtatttt atctgaaaag
     2101 tattttcacc gatcatacat tttgcatgtt caaacaagga tggaggaaag tagttgattg
     2161 gaaaattatc caattctttt tctgtaatta caaaccatcc atgatgttgt aatggatcca
     2221 ttttctgcat tattttttca gactttttca atgcaatagc agccttattt aatgtttgct
     2281 ttgattgaaa tactgaatga ttaagaatta ccaatgcatc aaagtcacta taatgaacca
     2341 actcattagt gcctaaacta ccatgtaaaa atgcacctaa tataaattta ttcaattttt
     2401 cattgaggta ttgtttcatt gcaagagcat gactgtaatt tatctgataa ttactaacca
     2461 aaatgctttt tgaacaagat gatacttcag tagagtagct gttgtacaat tttaatattg
     2521 atccggcatt ttttaaatag ggcagacggt agtgaattaa acttaataaa acatcagact
     2581 gatttaggca accatttttg aaatattttc tgactacttc aaagtgcttg ctgttttttg
     2641 ttgtcatatt ggaaattaca gcttttttga taccacaata attgcctgag ggcctagttt
     2701 atttgacagc cgaacaattg tttttagaaa aacattgtca atgaccaaga ccaatttcat
     2761 tacaagtttg cttttttgcc atgacttgta gaaacgattt attattgctt caaaaacaac
     2821 tctgaaaacg gaattataag gtacaataga aataatttga tgatttacat tttttaactc
     2881 tgccagtgat tccttcagat tgattttatt gtcatgatcg ctatgatcat aagttctgaa
     2941 aattttgcga accattgcac tttgtttttc tgagaattgt ctggaagtat aaactggctc
     3001 atgataggta aataatgcac cttgcgatag ttttttattt acctctgtga cagcctctgc
     3061 cggtcttggt aaatgatgta acatgccaaa actaattgca ccctgaaaag tattattgag
     3121 taaaggaata tttaaagcac ttgcctgaat aaagtcagat tccggaatag ccattttagc
     3181 taccattaat cgtttgaaag aaatatcagt gccaatgtaa ttgtatttgt atgtagcagg
     3241 gttaaacatt cttgcaacag ttcttgcatt tccacatcct atttcaaaaa tccattggcc
     3301 attacaatta acttcaagat gactaatcat tttctccttt cttggaataa atatccgcca
     3361 cagattatca ttttgaatag attgcaacat tttatctctt aattgcgcag tttcaacggc
     3421 tgcgtcataa tatttcattt cctcacgatt ccagttgcca tcagaagatt gatttagatt
     3481 ttctattctt aaaatatttt gttcaattgc ttgtataaat tcagtaggaa ttaaagacct
     3541 tactttttga tgtatttctt ttatatggat taaatcctga tcggaatcat ttaacaagat
     3601 cggaataaat tcatatataa agtatagatt tcttttgtcc tgtgtatata agattcctgt
     3661 cttaatatca tcattaggca atattgatgt gttgtcaagg tttgataata gctcctgctt
     3721 tcgtttaatt tcaagagcat aaagttgcaa cggttgcaaa ttggtatttg gacaaattag
     3781 tttttcgagt attcttttgt gcatttcaga tgtttactga tgagtgtatt gagataatta
     3841 attgagttgt tgaatgaata ttaaattcag ttgttctaag tataaatgac cttatttaca
     3901 aaatctttta cattaatttt attattaaac aaatcttgaa gtaaacgtat atattttaac
     3961 tgatcgattt gttgttcatc aaacatcttt ataataatat caatggcttt gaagccttct
     4021 aatactattg gatttccaag atcttttcca aaaaagcctt ttcctatcaa taagcctaaa
     4081 gttctgtttc tgcttaaatc atttaatgca gttaatccaa aatctccaat gccggcataa
     4141 ttaaaaagtg agtcgaatgg aattgccaat acttcagtaa tctttttaat ttcatttagg
     4201 gactttgtga ataccaaaaa gcgaacattt ggagagttgt agtatgcatc ggtaattcca
     4261 acaattattg catagatgtt ttttagaata cttaaaaatt caacacctct gatatctgtt
     4321 gtgaaatcaa aaactgttaa ttcgcttctg aaaagttcct tgattctatt aaaatctttt
     4381 tcataagctg ttgctattgt aaaagaagtt ggaatttcaa atatcaactc attggcaaat
     4441 gatggcccct ttaatgatgc aatttcgttt ggcagtaaat caccaagcag ttcaggaata
     4501 agtttgttgt atgtgccaaa tccttttgaa ccattaatga taatgcaatc ttcattcagg
     4561 tttaaagttt ttacatagcc ttcaacagca tgtgatggaa tacataaaaa aagaaagtca
     4621 gcttcattgg caaatgtaat atcgcttgtt gctttaattt tcttgctgag tatcttatta
     4681 ggaaaatagg aaatattttt acgttgtgta ttgatatcag aaattacctg cttatttctt
     4741 gtgtgaatgg ttatttgatt ttcctttttc aaggcattgg caagagc
//
LOCUS       DAOOOL010000167         4776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_1953_length_4776_cov_73.917390, whole genome shotgun sequence.
ACCESSION   DAOOOL010000167 DAOOOL010000000
VERSION     DAOOOL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1953_length_4776_cov_73.917390"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2672)
                     /locus_tag="PKU82_10740"
     CDS             complement(24..2672)
                     /locus_tag="PKU82_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83391.1"
                     /translation="MNRIVVSSIISIVIIGLMAVGYYYFSAYREKFSATEDALPSDAA
                     IVIRGSMGDLYASLEKVGLWNERDSLTVMYRFKKEMHTLLSNTTSMFDVADLLESPQT
                     TIAVLATGARNAELLYLVPSRKELNIDALQKALTGQLSENKSRTFAGVSIYELQFSNF
                     SSSFTFAFANGIFIGSFNAGLVEDAIRQQATGKPFGGNVSAQKLYKEIAVSSGTILGL
                     NYPALKKLFSVAISSDNVIELNNLEKWTEWSFHNLTFDGNSINANGSVLVSDSTQQIA
                     DFGNVRSSTDLLWSVLPDDVSALQIFSFKDALIWSDKNQRRNADNEVIINYIKQVSVT
                     ETKLRVEIRKDFSTIVGEIGAIAINGNPTSLLENNSVAILQLKDQKKSLTQLLALSKK
                     LSKNKDVIAVEKFRNHEIRFLNCDGLLPAMFGNAFNVIRKNYFTTHNGFVIFANKPSQ
                     LRGFIDDVEDNEMLNKNEVYQQNKSVLSESLSYALFLRPTNLNSYFQSVANSNTLKAL
                     AASDLMYKFSSFYYYIQPGISGKVDCNFRIVIQQQKQQKLSEPKWSFFTDSAIHCGPY
                     IFTSDNGISILFQDSTKSLICLDGSGNVSWKTTISGFINGEISQTINDQSGMIQFICN
                     TNDSMYMFNQNGVMPAHYPFKLPVHSRYGIVASPNTNVLFVAGENNLMYAFDPGSRPS
                     QNWNTVQFEGNLFNASIDALGRFVYIMDGDKLRLYSTDGKLILSKPSTEFRVNSMQWV
                     NDSVPSLFMLNNDGKIVVWNIDGTYKIASSDSTFDFAETCGSGTARIWYTIKGRELNI
                     LDYTFKAVENVDLSKVEFNNMQLFNTGNEILCILKGSENITIYRGDLKIVPQVIPAAD
                     FAVKTFQGKTYLISRSGNGKLTFYVL"
     gene            complement(2775..4685)
                     /gene="acs"
                     /locus_tag="PKU82_10745"
     CDS             complement(2775..4685)
                     /gene="acs"
                     /locus_tag="PKU82_10745"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOZ83392.1"
                     /translation="MYPYQITSAEQYTSVYRNSIENPEKFWANIAQYFTWQKAWDKVL
                     DADFEKAEVKWFAGAKLNITENCLDRHIEKLASQPAIIWEPNDPTEHHRVLTYKELLF
                     KVKQFANVLKNNGVKKGDRVCIYMGMIPELAIAVLACARIGAIHSVIFGGFSARSIAD
                     RLQDADARFIVTCDGAFRGAKDIPLKAIIDDALESCPFVQRVIVCTRTHTPVSMIKGR
                     DVWWEDEIKIVETQGNPDCIAEVMDAEDVLFILYTSGSTGKPKGVVHTVAGYMVWTNY
                     TFVNVFQYKPGQIYFCTADIGWITGHSYLLYGPLSAGATTMMFEGIPTFPDAGRFWDI
                     TQKHKVDILYTAPTAIRSLMAYGTSYLEGKDLSSLKVLGTVGEPINEEAWYWYYENVG
                     KSKCPIVDTWWQTETGGIMISNLAGITPHKPTYATLPMPGVLPCLVDETGNELIDNGV
                     SGNLCIKQAWPGMIRTTYGDHERCRQTYFSTYKNMYFTGDGCLRDKDGNYRITGRVDD
                     VLNVSGHRIGTAEVENAINMHQGVVESAVVGFPHDIKGQGIYAFVIFNGMHVDEEHTR
                     QDVIQTVSRIIGAIAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGELNNLGDTTTLLD
                     PGVVEEIKSGRL"
BASE COUNT         1517 a          975 c          748 g         1536 t
ORIGIN      
        1 tacaagttta ccaaacaaaa taattaaagt acataaaagg taagtttacc atttcctgaa
       61 cgagaaatca aataggtttt cccctgaaat gttttaacag caaaatccgc tgcaggaatt
      121 acctgtggta ctatttttaa atcacctctg taaattgtaa tattttcaga tcctttaagg
      181 atacataaaa tttcatttcc tgtattaaaa agttgcatgt tgttaaactc aactttactt
      241 aaatctacat tttcaactgc tttgaatgtg taatccaata tattcaattc cctaccttta
      301 atagtgtacc aaattcttgc tgtacctgaa ccacatgttt ccgcaaaatc aaaagtactg
      361 tcgcttgatg caattttata agtaccatct atattccaaa caacaatttt tccatcatta
      421 ttcagcataa agagtgatgg cacagagtca ttgacccatt gcattgaatt tactctaaat
      481 tcagttgacg gcttcgataa aatcagtttt ccatctgttg aatatagtct taacttatcg
      541 ccatccatta tgtaaacaaa cctacctaat gcatcaattg atgcattaaa taagttgcct
      601 tcaaattgaa ctgtattcca attttgtgaa ggtctgctgc ccggatcaaa cgcatacatt
      661 aagttattct cacctgctac aaaaagaaca ttagtatttg gtgatgctac aataccataa
      721 cgtgaatgaa caggcaattt aaacggataa tgtgccggca taacaccatt ttgattaaac
      781 atatacatgc tgtcgttagt attacaaata aattgtatca tacctgactg gtcatttata
      841 gtttgagaaa tttctccgtt aataaatccg cttatagttg ttttccaact tacattacca
      901 ctgccatcca gacagattaa actttttgtt gaatcctgaa acaagattga aatgccattg
      961 tcgctggtaa aaatatatgg tccgcagtgt attgcagaat ctgtaaaaaa cgaccacttt
     1021 ggctcagaca atttttgttg tttttgttgc tgtataacaa ttctaaagtt acagtcaact
     1081 ttcccggaaa tacccggttg aatgtaataa taaaaactac tgaacttata catcagatct
     1141 gatgccgcca atgcttttaa tgtattactg tttgcaacag attgaaaata tgaattaaga
     1201 ttagttggtc ttaagaataa agcataagac aagctttcag ataaaactga cttattctgc
     1261 tgatacactt cattcttatt aagcatttca ttatcttcaa catcatcaat aaacccacgt
     1321 aactgacttg gcttattagc aaatattaca aaaccgttat gagttgtaaa ataatttttt
     1381 ctgataacat taaaagcatt tccaaacatt gccggtaaga gtccatcaca atttaaaaag
     1441 cgtatttcat gattacgaaa tttctctaca gcaataacgt ctttattttt tgaaagcttt
     1501 ttgcttagtg ccagtaattg agtcaatgac tttttctgat cctttagctg caatattgcc
     1561 actgaattat tctcaagcaa tgaagttgga ttaccattaa tggcaatagc tccaatttct
     1621 ccaactatag tcgaaaaatc ttttcttatt tccaccctaa gctttgtttc tgttacagat
     1681 acttgcttta tatagttgat gattacttca ttatctgcat tccttctttg atttttatca
     1741 ctccaaatta gagcatcttt aaaggaaaaa atctgtaatg cagaaacatc atcaggcagc
     1801 actgaccaca acaaatcagt tgaagaacgc acatttccaa aatctgcaat ttgttgtgtt
     1861 gaatctgata caagcactga gccattggca tttattgaat tgccatcaaa tgtcagatta
     1921 tgaaaagacc attctgtcca tttttcaaga ttatttaact caataacatt gtctgaactt
     1981 attgcaacac taaaaagttt tttcaatgca ggataattga gaccaagtat tgttcctgat
     2041 gaaacagcta tttctttgta cagcttttgt gccgaaacat taccaccaaa gggcttacct
     2101 gttgcctgtt gcctgatagc atcttcaacc aaacctgcgt taaagcttcc aataaaaatg
     2161 ccattggcaa aagcaaatgt aaatgatgaa ctaaaattac taaactgaag ttcgtaaatg
     2221 ctgacaccgg caaatgttct tgatttgttt tcgctcagct gacccgttaa tgccttttgt
     2281 aatgcatcaa tatttaattc cttacgtgaa ggaacaagat aaagcagttc tgcattacgt
     2341 gctcctgtag ctaaaactgc aatggttgtt tgtggtgact caagcaaatc ggcaacatca
     2401 aacatacttg ttgtgttact cagcaaagtg tgcatctctt ttttaaaccg atacattact
     2461 gttaatgaat cccgttcatt ccacaaccct actttttcta aagaagcata caaatcgccc
     2521 atactacctc tgattactat ggcagcatca ctaggcaatg catcttctgt agctgaaaat
     2581 ttttccctat aggcagaaaa ataataatag cctacagcca tcaggcctat gatgacaatt
     2641 gaaattatgg aagaaactac aatcctgttc acaaataaaa cttgtgagtc aaaactatag
     2701 cttcactact cggagaaaca ttagtagtgt ttaatgttat ttacaagata atgttaattg
     2761 acaacaagca ctgtttacaa tcttccggat ttaatttctt caacaacacc cggatcgaga
     2821 agtgttgtag tatcgccaag attatttaat tctccttcag caattttgcg taatattctt
     2881 cgcataattt ttccgctgcg tgttttagga agtcctgaaa caaactgaat tttatcaggc
     2941 ttagcaatgg caccaataat tcttgacact gtctgaatta catcttgcct tgtgtgttcc
     3001 tcatcaacat gcattccgtt gaaaataaca aaggcataaa taccctgccc ttttatatca
     3061 tgtggaaaac ctactactgc actttcaaca actccctgat gcatgttaat tgcattctct
     3121 acttcggcag tgcctatacg gtgaccactg acattaagca catcatcaac acgtcctgta
     3181 attctgtagt taccatcttt atcgcgcaga cagccatcac ctgtaaagta catgttttta
     3241 taagtcgaaa aataagtctg acggcaacgt tcatgatcac cataagtggt acgaatcatt
     3301 ccaggccatg cctgcttaat acataaatta ccgctgacac cattatcaat aagttcattg
     3361 ccggtttcat caacaagaca tggaagcaca ccgggcatcg gcaatgtggc ataagtaggt
     3421 ttgtgaggcg ttatgcctgc aagattcgaa atcattatgc caccggtttc agtttgccac
     3481 caggtatcaa caattggaca tttacttttg cctacattct catagtacca ataccaagcc
     3541 tcttcattga ttggctcacc aacagtacca agcactttaa gtgaacttaa atctttccct
     3601 tcgagataag aagttccata agccattaag cttctaatag cagtaggtgc tgtatataaa
     3661 atatctactt tgtgtttctg cgtaatatcc caaaaacgac ctgcatcagg aaatgtcggg
     3721 attccttcaa acatcatggt tgttgctcct gcacttaaag ggccatagag cagataacta
     3781 tgtcctgtaa tccaacctat gtcagcagta cagaaataaa tttgtccggg tttgtattga
     3841 aacacattga caaaagtgta gtttgtccac accatatatc cggctactgt atgtactact
     3901 cctttaggct ttcctgttga acctgaagta taaagtataa acaacacatc ttcagcgtcc
     3961 atcacttcgg caatgcagtc ggggttgcct tgtgtttcaa caatttttat ttcatcttcc
     4021 caccatacat ctctgccttt aatcatactg acaggagtat gtgtgcgtgt gcacacgatt
     4081 acacgttgta caaacggaca actttcaagt gcatcgtcaa tgattgcttt caacggaata
     4141 tcctttgcac cacgaaaagc tccatcgcag gtaacaataa aacgggcatc ggcatcctgt
     4201 aagcggtcgg caatagaacg tgcagaaaag cccccaaaaa ttacagaatg aattgcgcca
     4261 attcgtgcac atgcaagaac tgcaatagcc agttcaggaa tcatgcccat atagatacaa
     4321 acacggtcgc ctttttttac gccattattt tttagaacat tagcgaattg ttttacttta
     4381 aacagcagct ctttgtaagt taaaacacga tgatgttctg tagggtcatt gggctcccag
     4441 ataatagcag gttgtgatgc taatttttca atgtgcctgt caagacagtt ttcagtaata
     4501 ttaagttttg ctcctgcaaa ccatttcact tctgcttttt caaaatcagc atctaaaact
     4561 ttatcccatg ctttctgcca tgtaaaatac tgtgcaatgt ttgcccaaaa tttttcagga
     4621 ttttctatac tatttcgata aacgctagtg tattgttctg ctgatgttat ctgatatgga
     4681 tacatttgaa taaaacttta aaaaatcaca gccacgaata taatacaaat tggaacttga
     4741 ggttcaattc aattgatgaa tcacttaaaa gtaaaa
//
LOCUS       DAOOOL010000168         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2018_length_4686_cov_74.879940, whole genome shotgun sequence.
ACCESSION   DAOOOL010000168 DAOOOL010000000
VERSION     DAOOOL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2018_length_4686_cov_74.879940"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1555
                     /locus_tag="PKU82_10750"
     CDS             176..1555
                     /locus_tag="PKU82_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOZ83393.1"
                     /translation="MITVIIPTLNEEENIASVIKFAKQNSLVSEVLVVDDKSIDNTVR
                     IAQDNDARVITSTKIGKGASMREGVMFSKNEIIVFLDADINPYPHFTIKLLTDPIIHS
                     GAGFVKSSFNRNAGRVTELVAKPMLSIFFPDLLRFNQPLSGMIASKKSLLQQLDFRDD
                     YGVDIGLLIDMHLIHVNISEVEIGYIENKSKPWQALGKMSKEVAQTIIIKASSSGNPH
                     YNFEEFGVLSEIRSQMEFALNKQQETFNKLLIFDMDNTLLRGRFIDTCAERYGFKKQL
                     MDIRSSETDSTIITKRIAKLLKGRTIDELIAIADGISIVDDTEKIITKLKKRGYVVGI
                     ISDSYDCITNHVRNKLRMDFSLSNELEFSKSVCTGEVKIPSLFVNNESSSCKHVLCKT
                     NALQKLLEKYDIQKENSIAIGDSMNDLCMIKEAGLGIAFCSKDELLNHHADVIIRKPD
                     FSALLNIAK"
     gene            complement(1644..2240)
                     /locus_tag="PKU82_10755"
     CDS             complement(1644..2240)
                     /locus_tag="PKU82_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83394.1"
                     /translation="MKTFLLASALFLSTSTFAQDKPELGNNIFSLTPMAIIGNESMDN
                     NTDVAVGLNYERILNEGLVSIKIPVNISLRNSGFFYIMPTIKLYPTRQGVVRYAVGPQ
                     FYIASGQINHHDYNTVWNGNQYITVNNKYSDNHTQLGFMINNSLNVSIAKTLYIGMEL
                     GLGIKYYDSNEENEYENSFDTTPVEPNVQFSFAMGYRF"
     gene            2379..>4686
                     /locus_tag="PKU82_10760"
     CDS             2379..>4686
                     /locus_tag="PKU82_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ83395.1"
                     /translation="MSDQTQIMQKKELSFEDFRQVVLNDYRLGRISRETSLLGRREVL
                     TGKAKFGIFGDGKEVAQIAMAKVFQNGDFRSGYYRDQTFMMAAGLLTVQEFFAQLYAH
                     PSVMAEPATAGRAMNGHYATRLLNDDGSWKNQTQSKNSSADISPTAGQMPRLLGLALA
                     SKFYRNNPELKSDKFKNFSVDGNEVAFGTIGDASTSEGLFFETINAAGVLQVPMVISI
                     WDDGYGISVPNKYQTTKQSISEALQGFARTQEKPGYEIIRAKGWDYAELCDAYEQAAN
                     IARTEHVPVMVHIQQMTQPQGHSTSGSHERYKSKERLLWEEEFDCLRKMREFMVQSAI
                     ATEQELDAIDAEAKKFVNDGKKQAWDSFTSEINTEKQQALSLAEAAISKSSNGVFLNK
                     AVEEIKNITNCERRDIAVFIQQVLFTLRADNSAEIEALKQWYRQWHQLNFDRYSSQLY
                     AEGNGSALGIAEVKAVYSAEANMVDGREVLLANHDKLFSQYPEYIAFGEDVGTIGGVN
                     QTFAGMQAKYGEHRVFDVGIREATIAGQGIGMAVRGLRPLAEIQYLDYLAYAIQILSD
                     DLACLRYRTKAGQKAPLIVSTRGHRLEGIWHSGSPMQFILGALRGMLVLVPRNMTQAA
                     GFYNLLLQCDDPALVIEPLNGYRLKEKLPENLGAFTVPVGVPEILEEGNDVTLVTYGS
                     CVRVAQDAMKQLQQCGISVELIDVQSLIPFDIHHIIAESIQKTNKVVFFDEDVPGGAT
                     AYMMQQVIENQKAFEFLETPPVTLAAKEH"
BASE COUNT         1527 a          763 c          972 g         1424 t
ORIGIN      
        1 cggatatata acttattcag atagttttta aattatactt atttttacat cagatttcct
       61 cagtgtattt ttcaaatacc aaatcggcac attcgtgttc agatagatga gattagtttt
      121 attataaaac taaaataaaa ataaacataa aaactaaaaa atttgtttaa taaagatgat
      181 tacagttatt ataccgacac ttaatgaaga ggagaatatt gcaagtgtta taaaatttgc
      241 aaaacaaaat tcattggtat cagaagtatt ggttgttgat gacaagtcta ttgataatac
      301 tgtaaggatt gcacaggata atgatgcaag ggtaattacc agtacaaaaa taggaaaagg
      361 agcatctatg cgcgaaggtg tcatgttttc aaaaaatgaa attattgtat tcttagacgc
      421 agatatcaac ccttatccgc atttcactat taaactttta acagatccaa ttattcatag
      481 tggtgccgga tttgtaaaat cgtcatttaa ccgaaatgca ggaagagtaa cagaattggt
      541 agcaaaacca atgttaagca tcttttttcc agacttatta agattcaatc agccattaag
      601 cggaatgatt gccagtaaaa aatccttgct tcaacaattg gactttcgtg atgattatgg
      661 tgtagatatt ggattgctga ttgatatgca tctgatacat gtaaatatca gtgaagtaga
      721 aataggttat attgaaaaca aaagtaaacc ctggcaagct ttaggaaaaa tgagtaaaga
      781 agttgcacaa actataatta ttaaagcatc atcatcaggt aatccacatt ataattttga
      841 agagtttggt gtgctgagtg aaattcgttc acaaatggaa ttcgcattaa ataagcaaca
      901 ggagactttc aataagttat tgatatttga catggataac acacttctgc gtggtagatt
      961 tattgatacc tgtgcggaaa ggtatgggtt taaaaagcag ctaatggaca ttcgttcttc
     1021 agaaacggat agtaccataa ttaccaaaag aatagcgaag ttgttaaagg gaaggacaat
     1081 agatgaactc atagctatag cagatggtat ttccattgtt gatgacacag aaaagattat
     1141 cacaaaattg aaaaaacgtg gctatgttgt tggaattatt tctgacagtt atgattgtat
     1201 aaccaatcat gttagaaata aattgcgaat ggatttttca ctttctaatg aattagagtt
     1261 ttcaaaaagt gtttgcactg gagaggttaa aataccctca ctgtttgtga ataatgaaag
     1321 cagtagttgt aaacatgttt tgtgtaaaac caatgcatta caaaaattac ttgaaaaata
     1381 cgatattcaa aaagaaaata gcattgccat tggagacagc atgaatgatc tttgtatgat
     1441 aaaagaagcc ggcttgggaa tagctttttg ctctaaagat gaattgttaa atcaccatgc
     1501 tgatgtaata attcgtaaac ctgattttag tgcattgctg aacattgcaa aataattttg
     1561 tcttttttga tttagataaa tagaataatt gaaattcttt aatctatctt gttccgaaac
     1621 cgacaaagtg tttaaagaaa agactaaaac cgatatccca tcgcaaaact aaactgcaca
     1681 ttaggttcta caggtgtagt gtcaaacgag ttctcatatt cattttcttc gttggaatca
     1741 taatatttaa ttcctaatcc cagttccata ccaatatata gcgttttagc gattgataca
     1801 ttcaggctgt tattaatcat aaaacccaac tgtgtatgat tgtctgaata tttgttatta
     1861 actgtgatat attgattgcc attccaaaca gtattgtaat catgatgatt aatttgtccg
     1921 ctggcaatat aaaactgtgg ccctacagca taccttacca caccttgtct tgtagggtag
     1981 agtttaatcg tgggcatgat gtaaaaaaat cccgaattac gtaatgaaat gttaacgggg
     2041 attttaatac tcaccaatcc ttcgtttagt attctctcat agttaagtcc cacagctaca
     2101 tcggtattat tatccataga ctcattgcca ataatagcca ttggtgtaag agaaaaaatg
     2161 ttatttccca attcgggttt gtcctgggca aatgttgatg tagataaaaa tagtgcagat
     2221 gctaagagaa aggttttcat atttttatgt tattttaaaa gctaaagtag tgtttttaag
     2281 acttcaaaaa aacggcattt aaattcaaaa attgtatggt tggcactttc agaatttcag
     2341 tacattcgtt acctttgtaa ctattaaaaa gcggaattat gtcagaccaa acccaaataa
     2401 tgcagaagaa agagctttca tttgaagatt tcagacaggt ggtgctaaac gactatcgtt
     2461 tagggcgtat cagccgagaa accagtttgt tgggacgcag agaagtgctg acaggcaagg
     2521 caaagtttgg catttttggc gatggaaaag aggtggctca gatagctatg gccaaggttt
     2581 tccaaaatgg cgatttccgc tccggttact atcgcgatca gacatttatg atggcagcag
     2641 ggttgctcac agttcaggaa ttttttgctc agctttatgc gcatccttct gtaatggcag
     2701 agcctgcaac agcaggtcgc gccatgaatg gacactatgc tacacgatta ctgaatgatg
     2761 atggtagctg gaaaaatcag acacaatcaa aaaattcttc tgccgacata tcgccaacgg
     2821 caggccaaat gccacgattg ctcggacttg ctttagcttc taaattctat cgcaataatc
     2881 cggaacttaa gtcggataag tttaaaaact tttctgttga tggtaatgaa gttgcttttg
     2941 gaactattgg tgatgccagc acttctgaag gtttgttttt tgaaaccatc aatgcagcag
     3001 gtgttttgca ggtgccaatg gttatcagca tttgggatga tggttatggt atttctgtgc
     3061 ccaataaata tcagacaaca aaacaaagca tcagtgaagc attacaaggt tttgctcgta
     3121 ctcaagaaaa acccggttac gaaattatta gagcaaaggg atgggattat gcagaattgt
     3181 gtgatgctta cgaacaggct gctaacattg cacgtacaga gcatgtgccg gttatggtgc
     3241 atattcagca aatgacacag ccgcaaggtc actctacatc aggatcgcat gagcgttaca
     3301 aatctaaaga gcgcttgttg tgggaagaag aatttgactg tctcagaaaa atgcgtgagt
     3361 ttatggtgca gtctgccatt gcaacagaac aagaactcga tgctatagat gctgaagcca
     3421 aaaagtttgt taatgatgga aagaagcagg catgggatag ttttacttct gaaattaata
     3481 ctgaaaaaca acaggcactt tcactggcag aagcagcaat cagcaaaagt tcaaatggtg
     3541 tttttctgaa taaagcagtt gaagagatta agaatatcac caattgcgaa agaagagata
     3601 ttgctgtttt tatacagcaa gttttgttta cgcttcgtgc tgataatagt gctgagatag
     3661 aagcactgaa gcaatggtac cgccaatggc atcagttgaa ttttgatcgt tacagctcac
     3721 aactttatgc agaaggtaac ggcagcgcat tgggcattgc cgaagttaag gctgtatatt
     3781 ctgcagaggc aaacatggtt gatggtcgtg aagtacttct tgcaaatcat gataaacttt
     3841 ttagtcaata tcctgaatac attgcctttg gagaagacgt tggaacgatt ggtggcgtta
     3901 accaaacttt tgcaggcatg caggccaagt atggcgagca tcgtgttttt gatgtaggta
     3961 tccgtgaggc aacaattgca ggacagggaa taggaatggc tgtgcgaggc ttgcgaccac
     4021 ttgcagaaat tcagtatctc gattatttgg cttatgcaat acagatttta tcagacgatt
     4081 tagcttgttt gcgttatcgc accaaggcag gacagaaagc accactcatt gttagtactc
     4141 gtggacatcg gttagagggc atctggcatt ctggttcacc tatgcagttt attcttggtg
     4201 ctttgcgtgg tatgcttgtg cttgttcccc gcaacatgac acaggcagca ggattttata
     4261 atttgctatt gcaatgtgat gatcctgcat tggtgataga accattgaat ggctacagac
     4321 taaaagaaaa actccctgag aacctaggtg cttttactgt gcctgtcggt gtgcctgaaa
     4381 ttcttgaaga aggtaatgat gttactctgg taacttacgg ttcatgtgtt agggttgctc
     4441 aggatgcaat gaaacaactg cagcaatgtg gcattagtgt agagttgata gatgtacagt
     4501 cactcatacc atttgacatt catcatatca tcgctgaatc tattcaaaaa acgaataagg
     4561 ttgttttctt tgatgaagat gttcccggtg gtgcaactgc atacatgatg caacaggtta
     4621 ttgaaaatca aaaagcattt gagtttcttg aaacaccgcc tgtaactctt gctgcaaaag
     4681 aacatc
//
LOCUS       DAOOOL010000169         4609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2066_length_4609_cov_47.518665, whole genome shotgun sequence.
ACCESSION   DAOOOL010000169 DAOOOL010000000
VERSION     DAOOOL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2066_length_4609_cov_47.518665"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /gene="sprA"
                     /locus_tag="PKU82_10765"
     CDS             complement(<1..2090)
                     /gene="sprA"
                     /locus_tag="PKU82_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOZ83396.1"
                     /translation="MLRYSVLSLFAFPLMYVNGGGSPSSFSDYSDLTGTRTIPLPVDT
                     PVNLPYPFNDRITDPYSNPYDHSPLYGTTPSNVNTSVEYNPETREYDISEKIGKDFFR
                     NPSYMTFEEFKEDQFRKSTQQYWKERAEGEDAITRKPLIPKIYVAGETFDRIFGGNTI
                     DIRPQGSAELTFGVNINRTDNPQIPEKQRRLTSFDFNEKIQMNVIGNIGEKMKLSVNY
                     NTEASFDFENQMKLEYTGYEDEIIRKIEAGNVTLPLTGTLIKGSQSLFGIKTQLQFGR
                     LGVTTIFSQQKGQASTINVPPGGGQVTNFDIDGDQYEANRHFYISQYFREHYDEAVSK
                     LPTIVSPISITKIEVWITQVNFNSQDQTRNLVAMQDLGEYNAYASSFVGQGTSVYPSD
                     QLSNNLYQKLTTTYSGIRNFQSASSVLDPLSSVYNFSPQQDYELITNAKKLSSTDFTY
                     NEKLGFISLNSELRNDQALAVAFEYTVNGKVYRVGELTTSGIDPSQPLIVKLIRGRNF
                     NTTLPSWKLMMKNVYNLNAFQISSEKFRLDVLYFDNTAGSYVRQLPEPNEPNIANKSL
                     IRVLNADNLNSTGDATQSGDGIFDYVEGITVNSANGRIILPAVEPFGRYLESKFSNST
                     TAAKYVFKELYDSTKTIALAQGKNKFKLKGSYQSSSGSEISLNAINIPQGSVKVTAGG
                     TELVENQDYTVDYNL"
     gene            complement(2268..2852)
                     /gene="ruvA"
                     /locus_tag="PKU82_10770"
     CDS             complement(2268..2852)
                     /gene="ruvA"
                     /locus_tag="PKU82_10770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOZ83397.1"
                     /translation="MYDYISGKITHATATYLIVEANGVGYQLQVSLQTYSKLKDAQSA
                     KVYTHLSIKEDAHLLFGFVDEDERQLFRHLISVSGVGPGTARVMLSSLSTSELQSAIT
                     TGNVAVIKKIKGIGDKTAQRIVIDLKDKLGKAGTSAPVLLTQGNTIKEEALSALLTLG
                     FNRNVAEKELNKVLAGSGEVQSVEVLLREALKNL"
     gene            complement(3233..4432)
                     /locus_tag="PKU82_10775"
     CDS             complement(3233..4432)
                     /locus_tag="PKU82_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOZ83398.1"
                     /translation="MKNLLTLLLLLSILNLRADYWTQKANFPGIAREAPFSFSIGNSG
                     FVGGGRDAFSVFHNDLWEYYPTTDSWTQKANFGGTPRYAAIGFAIGNKGYVGVGFDFL
                     GVLKQDFWEYNPSANVWTQKANFGGGNRYLAIGFSINNFGYCGTGYSTITTISNDFWE
                     YNPINDTWLQKTNMTGYYRAGANSFVIGNKGYVVGGFDTTGSVFYSDLWEFNQSLNSW
                     SQKSNLPSGGRCDASSFSICGKGYLGVGLDNGIVLNDLWQYNSVSNTWVSKANFPGLN
                     RYMAAFFSIGDKGYIGLGANGLFQNDFYEYTPDSLCATNIKEENNFNKSMFVFPNPIN
                     NELNISSNDKVQKEIILYDVSSKKLLQQKFTNSISLNTEGLAKGIYIYEVRDKNGSFK
                     KGKVVKD"
BASE COUNT         1390 a          863 c          828 g         1528 t
ORIGIN      
        1 agattatagt caaccgtata atcctgattc tcgaccaatt ctgttccacc ggcagtaact
       61 ttaacagagc cttgcggaat gtttatggca ttgagagaaa tttcagatcc tgaagagctc
      121 tgatatgagc ctttaagttt aaatttattt ttaccttgtg ctaatgcaat tgtttttgtt
      181 gaatcgtaaa gctctttaaa cacatacttt gctgccgttg tagaatttga aaattttgat
      241 tccaggtatc gtccaaatgg ttctacagcg ggcagaataa ttcttccgtt agcactgttt
      301 accgttattc cttccacata atcaaaaata ccgtcaccgg attgtgtagc atcaccggta
      361 gagttcaggt tatcggcatt aagcactctg ataaggcttt tattggcaat gttgggctcg
      421 ttaggctcag gcaactgacg cacataactt cctgcagtat tgtcgaaata aagaacatct
      481 aatctgaatt tttcgcttga aatctgaaaa gcattcaggt tataaacgtt tttcatcatc
      541 agtttccatg atggaagtgt ggtgttgaag tttcttcctc tgataagttt tacaataagt
      601 ggttgcgatg ggtcaatacc cgaggtagta agttcaccta ctctgtaaac tttaccgtta
      661 acggtatatt caaatgcaac ggctaatgcc tgatcatttc tcaactcgga gttgagcgaa
      721 ataaatccta acttttcatt gtaggtaaaa tctgtactgc ttaatttttt tgcattcgtg
      781 attaactcat aatcctgctg tggcgaaaaa ttataaactg aagataatgg gtcgagtaca
      841 gaagatgccg actgaaaatt acgaataccg ctataagttg ttgtcagttt ctgatacagg
      901 ttgtttgaca actgatctga tggataaacg gatgtgcctt gcccgacaaa tgaagaagca
      961 taagcattat actctcctaa atcctgcatg gctacaaggt ttcttgtctg atcttgtgag
     1021 ttgaagttta cctgtgtaat ccagacttct atcttggtga tgctgatggg tgaaactata
     1081 gtaggcagtt tgcttactgc ttcatcataa tgctctctga aatattgaga aatataaaag
     1141 tgcctgttgg cttcgtactg atcaccatca atatcgaaat tagttacctg gccgccaccg
     1201 ggaggtacat tgatggtact agcctgacct ttttgctgag agaaaattgt tgtaacacca
     1261 agtcttccga actgcaactg tgtttttata ccgaacaaac tctggctgcc tttgattaat
     1321 gttcctgtta gtggtaaagt aacattacct gcttctattt ttctgataat ctcatcttcg
     1381 taacctgtgt attcaagttt catctgattt tcgaaatcga aacttgcttc tgtgttgtag
     1441 ttaacactga gtttcatttt ttctccgatg tttccgatta cattcatttg aattttctcg
     1501 ttaaaatcga aagaagtaag gcgtctttgt ttttcgggta tctgcggatt gtcggtgcgg
     1561 tttatgttaa caccaaaagt caactctgca gaaccttgcg gacggatgtc aatggtgttg
     1621 ccaccaaata ttctgtcgaa tgtttcgcct gcaacataaa ttttaggaat taatggtttg
     1681 cgggtaatgg catcttcacc ttcggcacgt tctttccaat attgctgtgt tgacttgcga
     1741 aactgatctt ctttaaactc ttcgaacgtc atgtaggaag ggttgcggaa aaagtcttta
     1801 cctatttttt cgctgatgtc gtactctctg gtttcaggat tgtattcaac tgaggtgtta
     1861 acattggaag gcgtagttcc gtaaagcgga ctatggtcgt aagggttgga atatggatcg
     1921 gttatcctgt cgttaaaagg ataaggaaga ttaacaggtg tgtcaacagg cagtggtatg
     1981 gtgcgggttc ctgtaaggtc tgaatagtct gaaaaagaag atgggctgcc tccgccattg
     2041 acatacatca acggaaatgc aaacaaactt aatactgaat agcgaagcaa ttttagtcct
     2101 tgcaaaatat ctgatatttg ggggtcctac aaatgagtgt taaaaaattt tccggctaaa
     2161 aataaaacaa aaaatcaaac ggtttaaagg taaaaatcaa cagttacgga cacttttttt
     2221 tcaacttcat ttttgttaaa atgaagcact tttttgaacg tccgtattta taaatttttt
     2281 aaagcctctc tcagtaaaac ctcaacagac tgaacctcac cactaccggc cagcacttta
     2341 ttcagttctt tttcggccac attacggtta aacccaagag taagcaaagc acttaacgcc
     2401 tcttccttaa ttgtattgcc ttgtgttaat aaaactggtg ctgaagtgcc ggcctttccc
     2461 aacttatctt taaggtctat gactatgcgt tgcgctgttt tgtcgcctat gcctttaatt
     2521 tttttgatta cagccacatt gcctgtcgta atggcacttt gcaattctga agtactgagc
     2581 gatgaaagca taactcttgc agtgccgggg ccaacacccg atactgaaat aagatgacgg
     2641 aaaagctgcc tttcgtcttc atcaacaaaa ccaaaaagca gatgtgcatc ttctttaata
     2701 gatagatgag tataaacttt ggcactctgt gcatctttaa gttttgaata ggtttgtagt
     2761 gaaacctgaa gctgataccc tactccattg gcttctacaa taaggtaggt ggcagttgca
     2821 tgggttattt ttcctgaaat ataatcgtac atctttttta aaaatttttt ctgctgctat
     2881 aaaaatgcga cctcaaactt acggaaagtt ggtgagcttc tgagttggag atcagccacg
     2941 gctgacaggt ttgctgagtt gatgagttgg aagaatttat tattagataa ttaaaaaaat
     3001 ttgaaaattt tgtatgttat atggtttgat attttctcag agtttccaaa tacaaaatag
     3061 tagaggtact atgtggacta agaaattaaa tatcactttt aaatcaaact gtaaggggct
     3121 acacatcatg tcaggtgaaa tattgatttt aacgctactc aactactgca attaaaattt
     3181 caatcactaa tatttacagg ggaatctttc tgttatcagc cgtctctctc tctcaatcct
     3241 tcacaacttt tcctttcttg aacgaaccat ttttgtcgcg cacttcgtaa atataaattc
     3301 cttttgcaag tccttcagta tttaatgaaa tcgagtttgt aaatttctgc tgcaataatt
     3361 tctttgaaga aacatcatat agaataattt ccttttgcac cttgtcatta gaactaatat
     3421 tcaattcatt attaattgga tttgggaaaa caaacattga tttattaaaa ttattttctt
     3481 ctttaatatt tgttgcacat aaactgtcag gcgtatattc atagaaatca ttttgaaaaa
     3541 gtccatttgc ccccaatcca atatatccct tgtctccaat tgaaaaaaaa gctgccatat
     3601 atctatttaa acctggaaaa tttgcttttg agacccatgt gttgctaaca gagttatatt
     3661 gccataaatc atttaatacg attccattgt ccaacccaac tcctaaatat ccctttccac
     3721 aaattgaaaa tgaagaagca tcacaacgtc caccagaagg taaattagat ttttggctcc
     3781 aactatttaa agattgatta aactcccata agtcagaata gaatactgaa cctgttgtat
     3841 caaagccacc aactacatat cctttattgc caataacaaa cgaattagca cctgctcgat
     3901 aatatcctgt catattcgtt ttttgcaacc atgtatcatt aataggatta tattcccaaa
     3961 aatcattgct tatggtggtg attgtgctat aacctgttcc acaatagcca aagttgttta
     4021 ttgaaaaacc aattgcaagg tatctattcc ctcctccaaa atttgccttt tgtgtccata
     4081 catttgctga tggattatac tcccagaaat cttgcttcaa aacgccaaga aaatcaaatc
     4141 cgactcccac atatcctttg ttgccaatag caaatccaat agccgcatat cttggtgtgc
     4201 cgccaaaatt tgctttttgt gtccatgaat cagtcgttgg gtaatactcc cataaatcat
     4261 tgtgaaacac agaaaaagca tctcttccac cgccaacaaa gccgctgttt ccaatggaaa
     4321 aagaaaaagg tgcttctctt gcaattccag gaaaatttgc tttttgtgtc caataatctg
     4381 cccttaaatt tagaatggat aatagtaata aaagagtcaa aagattcttc atagttgtaa
     4441 tatttgaata tatactaaca tttgttctgt ttattaaaaa gattaaaaac gtcagtctgt
     4501 caaatgaata tcgttaaaat agctatctga attaaatcta tacatcagtt caaaagtaaa
     4561 gagaaaagta attagattgt tgagaattaa taagtgaata gagagaagt
//
LOCUS       DAOOOL010000170         4588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2086_length_4588_cov_51.910876, whole genome shotgun sequence.
ACCESSION   DAOOOL010000170 DAOOOL010000000
VERSION     DAOOOL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_4588_cov_51.910876"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..2344
                     /locus_tag="PKU82_10780"
     CDS             89..2344
                     /locus_tag="PKU82_10780"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83399.1"
                     /translation="MTANGGKGGKVGYFWAQYGAGGASTAGTHSGGAGAAGASGGSGG
                     GGGGAGSTGNGGNASGTTGGTGGTGGGGTGANGRTSNGDGSDATALSAGGGGGRSNAS
                     NTDRIGGDGFRGQIKVTYTCVSYSLTATSGTTPICASAGTSVISLTGTAATLPVGDYV
                     VTYDRSLPSASNLTVSMAVVTAGTGSFTATGLTSGGNSTITVTKLTSSYCNNNITLNN
                     TTVIAVAASIPPQPDVIAGAPVDVCPPTASYTLSTNSTGADSYNWHLGVGVTGVTFLS
                     ANGNNTMDVQFGATANSTYVIRVDATNACGTSAYRPVMIRRATSVPAAITGLKTACAS
                     TSDTYTATAVAGATSYEWSISGDATVTGTTNTATVNFGPTWTGGTLCVASKVGCFTSA
                     TKCMTISTSATVLSTVLGGTFKACPNSSLNFNITASPGVATYNWTVPANASINSGQGT
                     NTITVDFGPSYNNTGNICMTATSICGVVAPTKCKSVSPGLPSVPASITGGTNGLCNQT
                     MPFSTPTMAGVTSYNWTAPGTVNNNGNNAVTIDFGALTTGQVCVSATNSCGTSNTRCT
                     AVKGAPNTPASITATPSTWCPNTAGVEFNANIANVTGAYTLSWTYPAASANYISGGGN
                     SSSLILDWTTGNGAVMITATNACGSGTKVFDVVLDNCREAGIETVKQMDVYPNPANDL
                     VNISYQSAATELIKVSLMDYSGKLVVNQNVQVNSGMNNYSLDVSKLAPGVYMLNVQSE
                     NGNMQRKLIKQ"
     gene            complement(2434..3318)
                     /locus_tag="PKU82_10785"
     CDS             complement(2434..3318)
                     /locus_tag="PKU82_10785"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOZ83400.1"
                     /translation="MQVTIDSNSGFCFGVVYAIQQAEEELEVNGHLYCLGDIVHNNME
                     VDRLKGKGLEIINHEQLQQLHDCKVLIRAHGEPPSTYQTAIKNNIELIDASCPVVLKL
                     QNRVRGSYDEITDTGNGQVVIYGEKGHAEVNGLVGQTGGKAIVVKTEEDLDQIDFNKP
                     VHFFSQTTKSTLGFEQMQKTIQQRIVKQKGSLEETDFITNDTLCRQVSNREPQMRRFS
                     SQHDVVVFVSGKKSSNGKALYSVCKATNDKSYFISSAEELDATWFQGINSVGICGATS
                     TPLWLMKDVKNAIEKMTG"
     gene            complement(3379..4143)
                     /locus_tag="PKU82_10790"
     CDS             complement(3379..4143)
                     /locus_tag="PKU82_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ83401.1"
                     /translation="MKITFLGTGTSQGVPVIACNCNVCQSTDLRDKRLRTSIHIQHKN
                     SSFVIDSGPDFRQQMLRAQIKNLTALIFTHEHKDHVAGMDDIRAFNYVLQKKIDIYAT
                     LRVQEALVREFPYVFHDFKYPGVPEVNMITIDEHPFNIEGLEFIPIEVLHYKLPVNAY
                     RVGDFTYITDANFISEKEKEKIKGSRIVVINALRREKHVSHFNLEEALELIKELKPEK
                     AYLTHISHQLGKHSDVEKELPPNVFLAYDGFEIEMS"
     gene            complement(4140..4469)
                     /locus_tag="PKU82_10795"
     CDS             complement(4140..4469)
                     /locus_tag="PKU82_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83402.1"
                     /translation="MALRTSKNDMAKFTPQYLKKLEEHLLENGYDVRYEKGNFKNGYC
                     LLESKKLVMVNKFSAMETRCSALLEIINLLESQQLLSNKRQELFAAMENPEPQVVTIP
                     HSSESEH"
BASE COUNT         1349 a          904 c          935 g         1400 t
ORIGIN      
        1 ccgatgtaaa agaaaaacca tcataccaaa caataaaaat gctgttgcct gatgaggtaa
       61 tgggggcaat tcatcgtttg gaggttcagt gacagctaat ggtggtaaag gtggtaaagt
      121 aggttatttt tgggcacaat acggtgctgg aggtgcctca acagcaggaa cacacagtgg
      181 gggtgccggt gcagcaggtg ccagtggtgg ctcaggaggc ggaggcggag gtgccggttc
      241 aacaggaaat ggtggcaatg catcaggaac aacaggtggt acaggtggta caggtggtgg
      301 aggtaccggt gcaaatggta gaacaagtaa cggtgatgga agtgatgcaa ctgcactttc
      361 agcaggtggt ggcggtggcc gctctaatgc tagtaatact gatagaatag gtggtgatgg
      421 atttaggggg cagataaaag taacctatac ctgtgtatct tatagcctta ctgctacaag
      481 tggtacaaca ccaatttgtg cttctgcagg tacttcagta atttctttaa caggaacagc
      541 agcaacattg cctgttggcg attatgtcgt tacctatgac agaagccttc cttcggcaag
      601 taatttaaca gtttctatgg cagtggttac agcaggtaca ggttcgttta cagcaacagg
      661 tttaacttca ggaggtaaca gcacaattac agttactaaa ctcacatcat cttattgtaa
      721 taacaatatc actttaaata atacaactgt tattgcagtt gctgccagta ttcctcctca
      781 acctgatgtt attgcaggtg cacctgttga tgtttgtcct ccaacagcaa gctacacact
      841 tagcacaaac agcacaggtg ccgattcata taactggcat ttaggtgttg gtgtaaccgg
      901 tgttactttt ttaagtgcta acggaaataa tactatggat gtacagtttg gagcaacagc
      961 taactcaacc tatgttattc gtgttgatgc aaccaatgca tgtggtactt cagcttatcg
     1021 tcctgtaatg ataagaagag caacctctgt tccagctgca attaccggac ttaaaactgc
     1081 ctgtgccagc acatctgata cttatacagc aactgcagtt gcaggtgcaa catcgtatga
     1141 gtggtctatt agcggagatg caactgtgac gggaactact aacacagcaa cagtaaactt
     1201 cggtccgacc tggacaggtg gaacactttg tgtagcttca aaagtaggtt gctttaccag
     1261 tgctactaaa tgcatgacta tttcaacatc tgcaacagtt ttaagtactg tgcttggtgg
     1321 aacatttaaa gcatgtccaa actcatcgct taactttaat attacagcat cacccggtgt
     1381 ggctacttac aactggactg ttcctgccaa tgcatctata aacagtgggc agggaaccaa
     1441 tactatcact gttgattttg gtccaagtta taacaacaca ggaaatattt gtatgacagc
     1501 aaccagcatc tgcggtgttg ttgcaccaac aaaatgtaag agtgtttctc cgggattgcc
     1561 ttctgtacct gcaagcatta caggtggtac caatggtttg tgtaatcaaa ctatgccatt
     1621 ttctacacca acaatggcag gtgtaaccag ctataactgg acagcacccg gaactgtaaa
     1681 taacaatgga aataatgcgg taacaattga ttttggtgct ttaacaacag gacaggtttg
     1741 tgtgagtgca accaatagct gcggaacaag caacacgcgt tgtactgctg ttaaaggtgc
     1801 acctaataca cctgcttcaa ttactgcaac accaagtaca tggtgtccta acactgcagg
     1861 agttgagttt aacgccaata ttgctaatgt aacaggtgct tatactttaa gctggacata
     1921 tcctgcagca tctgcaaatt atatttcagg tggtggaaac tcaagttcat taattcttga
     1981 ttggacaaca ggcaacggtg cagtaatgat tacggcaact aatgcatgtg gatcgggtac
     2041 aaaagtgttt gatgtagtat tagacaactg ccgcgaagca ggaattgaaa cagtaaaaca
     2101 aatggatgtg tatcctaatc ctgcaaatga tttggtaaac atttcatatc aatcagcagc
     2161 aacagagttg ataaaagttt cgttgatgga ttactccggt aaacttgtag taaatcaaaa
     2221 tgtccaagta aactcaggca tgaacaacta ttcattagat gtttctaaac ttgctcctgg
     2281 ggtttatatg ttaaacgtac aatcagaaaa cggcaatatg caacgtaagc tgataaaaca
     2341 ataattaatt attttaaaat taacaaggct gctttggtta cgaagcagcc tcgttttttt
     2401 tagtttactt ttcaaaaaaa ctaaaccaat cttttagcct gtcatttttt caatggcatt
     2461 tttcacatcc ttcatcaacc atagtggtgt tgaagtagct ccacaaatac caacagaatt
     2521 aattccttga aaccaagtgg catccaactc ttcagccgaa gaaataaaat aacttttgtc
     2581 gttggtagct ttacagacac tgtataaagc ttttccgttt gatgattttt ttccgcttac
     2641 aaaaactaca acatcatgtt gtgatgaaaa tctgcgcatc tgtggttcgc ggttgctaac
     2701 ctgacggcat aaagtgtcgt tggtaataaa atcggtttct tcaagcgagc ctttttgttt
     2761 tactattcgt tgctgaatgg ttttttgcat ttgttcaaaa ccaagtgtgc tttttgttgt
     2821 ctgagaaaaa aaatgaaccg gtttattgaa atctatctga tcaagatctt cttctgtttt
     2881 aactacaatg gcttttccac ctgtctggcc caccaaacca ttcacttctg catgaccttt
     2941 ctcgccataa atcaccacct gaccatttcc cgtatctgta atttcatcat aactgcctct
     3001 gacacgattt tgtaacttca gcacaacagg acaagatgca tcaataagct caatattgtt
     3061 tttaattgcc gtttgatatg ttgatggtgg ttcgccatgt gctctgatta gtactttgca
     3121 atcgtgcaac tgttgcaact gttcatgatt gataatttcc aaacctttac ctttaagtct
     3181 gtcaacttcc atgttgttgt gtacaatgtc gccaagacaa tataagtgac cgttaacttc
     3241 aagctcttct tcagcctgct gtatggcata aactacacca aagcaaaagc ctgaattgga
     3301 atcaatagta acctgcatag tgaacaaatt atgtgcaaaa gtaagacaat aacgttttaa
     3361 acgccctatg gtttaatctt atgacatttc tatttcaaaa ccatcgtaag ctaaaaacac
     3421 atttggcggt aattcctttt ctacatcact gtgttttcca agctgatggc tgatgtgtgt
     3481 gaggtaggct ttttcgggct tcaattcttt aatcaactca agtgcttctt caagattaaa
     3541 atgagaaaca tgtttttcgc gtcttagcgc attgataact acaatacgac tgccttttat
     3601 tttttctttt tctttttctg aaataaagtt agcatctgtt atgtaggtaa aatcaccaac
     3661 cctataagca ttgacaggta atttgtaatg cagcacttca atgggaataa actccagtcc
     3721 ttcaatatta aaagggtgct catcaatggt aatcatattc acttcaggca cacccggata
     3781 tttaaagtca tgaaaaacat aggggaactc tctaactaac gcttcctgaa cacgcaaagt
     3841 agcatagatg tcaatttttt tctgcaacac atagttgaat gccctgatgt cgtccatacc
     3901 tgcaacatga tctttatgct cgtgggtaaa aattaatgca gtaaggtttt ttatctgcgc
     3961 ccgcaacatt tgctgcctga aatcggggcc ggagtcaatg acaaaactgc tgttcttatg
     4021 ctgaatatga atggaagtgc gcaaacgttt gtcgcgcaaa tcagtactct ggcatacatt
     4081 gcaattgcag gctattaccg gaacgccttg cgaagtgcct gtacccaaaa aagttatttt
     4141 caatgttcag attctgatga atgaggtatg gtaactacct gtggttcggg attttccatt
     4201 gctgcaaaca actcctgcct tttgttgctg agtaattgtt gcgattcaag aaggttgatg
     4261 atttcaagta gtgcactgca tcgtgtttcc atggcagaaa atttatttac catcactaac
     4321 tttttggatt ccagcagaca atagccattt ttaaagttgc ctttttcgta gcgtacatcg
     4381 tagccgtttt ccaataaatg ctcttctaat ttttttaaat actgtggtgt aaacttagcc
     4441 atatcgtttt tagaggttct tagtgccaaa attattgagt atgatgttca ctattttgct
     4501 ttggcaggga gttttattaa caacggacaa cgatgataag agattgcata taagccgaaa
     4561 attattttct ttgcaccgct ttctgata
//
LOCUS       DAOOOL010000171         4538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2125_length_4538_cov_101.544948, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000171 DAOOOL010000000
VERSION     DAOOOL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2125_length_4538_cov_101.544948"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1612
                     /gene="atpD"
                     /locus_tag="PKU82_10800"
     CDS             110..1612
                     /gene="atpD"
                     /locus_tag="PKU82_10800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOZ83403.1"
                     /translation="MNKGKITQVIGPVVDVSFEGENTKLPNIMEALEIKRPDGTSVIL
                     ECQQHIGEDTVRAISMDATDGLVRGMEVVATGDAIKMPTGDAIKGRLFNVVGDAVDGM
                     KAVSKEKGLPIHREAPKFEDLSTASEVLFTGIKVIDLLEPYSKGGKIGLFGGAGVGKT
                     VLIMELVNNIAKAYSGLSVFAGVGERTREGNDLLREFIESGVINYGDDFKHSMEQGGW
                     DLSKVDYNKLTDSKATLVFGQMNEPPGARARVALSGLTVAEHFRDGDGTGSGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILTEEVVGKEHYET
                     AQKVKQLLQRYKELQDIIAILGMDELSDEDKMAVHRARRVQRFLSQPFHVAEQFTGLK
                     GVLVPIEETIKGFNMIMNGEVDEYPEAAFNLVGTIDDAIEKGKKLLAEAK"
     gene            1683..1922
                     /gene="atpC"
                     /locus_tag="PKU82_10805"
     CDS             1683..1922
                     /gene="atpC"
                     /locus_tag="PKU82_10805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOZ83404.1"
                     /translation="MFLEIITPDKKVFEGEVTSVSVPGADGKFQMLNNHAPIISTLGK
                     GKVKVKTAKETLEFNVKGGVVEMLKNKVVVLAESV"
     gene            2539..2832
                     /locus_tag="PKU82_10810"
                     /pseudo
     CDS             2539..2832
                     /locus_tag="PKU82_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
     gene            2902..3135
                     /locus_tag="PKU82_10815"
                     /pseudo
     CDS             2902..3135
                     /locus_tag="PKU82_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
     gene            3303..4415
                     /locus_tag="PKU82_10820"
     CDS             3303..4415
                     /locus_tag="PKU82_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HOZ83405.1"
                     /translation="MNRLISTYNWQHKNWPSFDYNTEVVSEITPMISNYIGEMSGIMQ
                     SLTDKDQTETLIRLITTEAISTSAIEGEILNKQDLMSSIKNNLGVNTKAESVKDKKAK
                     AIAELMISVRNEYSNKLTITSIKKWHKLLFLNSQAINAGKWRSGKEPMQVVSGRIGKE
                     IVHFEAPPSERVAKEMKLFTEWYNAYKITDNMTNAIVKSAITHLYFESIHPFEDGNGR
                     IGRAIAEKCFSQSLGRPVYLSLSSIIERNKKQYYLALKEAQGSLHINNWIVYFANIIL
                     QSQKEAIRTAQFALKKIIFLNNHALNLNEREQKVVNKMLEAGWDGFEGGMTAKKYMKI
                     TKSSKATATRDLQHLTELGVFNSKGNGRNIHYELTI"
BASE COUNT         1569 a          766 c          850 g         1353 t
ORIGIN      
        1 gttaggcagt tgttgacaac attcgttttt atttttcaaa gcaattaaaa ttaatgctac
       61 tttcgcgcct tgaaaaatca gttttcagaa cgaataaaaa gtataattca tgaataaagg
      121 taaaataact caggtaatcg gtcctgttgt tgacgtatca tttgaaggcg aaaacaccaa
      181 acttcctaat attatggagg ctttggaaat taaacgccct gacggaacaa gtgtaatttt
      241 agagtgccag cagcatattg gcgaagacac cgttcgtgcc atctctatgg atgccaccga
      301 tggtttggta agaggtatgg aagtagttgc tacaggcgat gccattaaaa tgccaacagg
      361 cgatgccatt aaaggacgtt tatttaatgt tgttggtgat gcagttgacg gcatgaaagc
      421 ggtttcaaaa gaaaaaggct tgcctattca ccgtgaggca ccaaaatttg aagacctttc
      481 tactgcctct gaagtacttt ttacaggtat taaagtaatt gaccttttgg agccatacag
      541 taagggtgga aaaattggtt tgttcggtgg tgcaggtgta ggtaaaaccg tattaattat
      601 ggaattggta aacaacattg ccaaagcata tagcggactt tctgtatttg ccggtgttgg
      661 tgagcgtaca cgtgagggta atgacttatt gcgtgagttt attgaatctg gcgttatcaa
      721 ttatggtgac gattttaaac atagtatgga acaaggcgga tgggatttga gtaaggttga
      781 ctacaacaaa cttaccgact caaaagcaac acttgttttc ggacagatga atgagcctcc
      841 cggagcgcgt gcacgtgtag ccctgtcggg acttactgtt gccgagcatt tccgcgatgg
      901 tgatggcaca ggtagtggcc gcgacatcct tttctttatt gacaacattt tccgtttcac
      961 gcaggcaggt tcagaagtat cggcactttt aggtcgtatg ccttctgccg taggttatca
     1021 gcctacactt gccacagaaa tgggactcat gcaagagcgt attacttcaa caaaaagagg
     1081 atctatcaca tcagtacagg ccgtatatgt acctgctgat gacttgactg acccagctcc
     1141 tgctacaaca tttgcccacc ttgatgcaac aacggtattg agccgtaaaa ttgcagagct
     1201 tggaatttat cctgctgtag atccattgga ttctacctct cgaattctta cagaagaagt
     1261 tgtgggtaaa gagcattatg aaactgcaca aaaagtaaaa cagttgttac aacgctacaa
     1321 agaacttcag gacatcattg ccattcttgg tatggacgag ttgagtgacg aagataaaat
     1381 ggctgttcac cgtgcaagaa gggtacagcg attcctatca cagccgttcc acgttgccga
     1441 gcagtttaca ggattaaaag gtgttcttgt gcctattgaa gaaactatca aaggctttaa
     1501 catgattatg aatggcgaag ttgatgagta tcctgaagca gcatttaacc ttgttggtac
     1561 tattgacgat gctattgaaa aaggcaaaaa actgttagct gaagcaaaat aatttaaatt
     1621 agataattgc gaaagtgagg ttatgtcatg tcattctcaa attcgtacaa attcgtaaat
     1681 aaatgttttt agaaattatt actcccgata aaaaagtgtt tgaaggcgaa gtaacgtctg
     1741 tctctgttcc cggtgctgac ggtaaatttc agatgcttaa caaccacgct cctattattt
     1801 caacacttgg caaaggcaag gttaaagtaa agacagcaaa agaaacgctt gagtttaatg
     1861 taaaaggtgg agtggtagag atgcttaaaa acaaagttgt tgttttagct gaatcagtgt
     1921 agattaaaaa tacaaattat aaaaaaagct acctcacaag ggtagctttt tttgtggggt
     1981 tgagttgtaa agtacgctat tacactaact attgtaaagc attaaaacat gctaatcatt
     2041 ttaaaattcc aatataaaat tggtcaaaat tttaaattca ttgaatgctt gaaaactgcc
     2101 ggcaaatgct tacttacaat cttaggtaac ggtttgacct ttgatagttt cttgatatag
     2161 ctcacttttt acataagaat agaacaaaat caatcatttt tgagcttaaa accaatcata
     2221 aatgagctta aagcataaag ataattatta agaaacgcct accaaataac atttttagta
     2281 gaaataatgg aagaagttct tcaaaagata tttttgaata atcaggcatt aaagtcaaat
     2341 atgattcttt taggcgcaat tttataaaat tgttatctac taattattta cttactaaag
     2401 gttatgctaa gtgtataaaa caaattattt ctctaatact tgaattaatt aaagctcaca
     2461 tccacactta tcttgatatg ttgtttgttt atctttaagc caaaaacaaa taaataccta
     2521 agtacaaaaa ctataataat gcgactatca aacaacatag ataaatttgg aactgccgga
     2581 cttttcctga cagctatatt ttctccctgt tgttttccac tatttgcatt tacagcatca
     2641 gcacttgggc tcggtagttt tgagttgttt ggcgggtgga cgatgtggat ttttcaggca
     2701 atggttttaa tttctgtgag cggactttat ttctcatatc gtaaacatca ttgtctgtat
     2761 cccttgttga ctgcaatcat aagcggactc ttaatatttt acgcatatta tattaacgac
     2821 agcgattatt ggataaatct actttacatt ggcatgttcg gacttttact ggcaacagta
     2881 tggaactaca aaagaaataa aatgcatgga acttgtaata cttgcataac ttataacggc
     2941 aaaacagtag aaacacaatc cacactgact tgtccaaact gcggacataa aaaaactgaa
     3001 actatgccaa ctgatgcatg tgtttatttt tatgaatgtg aaaattgtaa aacaagatta
     3061 aagcccttac aaggcgattg ttgtgtttat tgcagctacg gtacagttaa atgtccacca
     3121 tacaggcagg agagaattgt tgttaatata catgagttta ttaaacatcc tctagttttc
     3181 attgatgaaa aatcacaaac atgcaattgt tacaacttta ttattttcaa aaaaacattt
     3241 gctgagctat tttaaataat tattcggctc atatatttcg tatatttgag ccgaataatt
     3301 taatgaatcg gctcatttca acatataact ggcaacacaa aaactggcca tctttcgact
     3361 acaatactga agttgtatct gaaatcacac caatgatttc aaattacatt ggtgaaatgt
     3421 caggaataat gcaaagcctg actgataaag atcagacaga aactcttatt cgattaataa
     3481 caacagaagc aattagcact tcggcaatag aaggagagat attaaacaaa caggatttaa
     3541 tgtcttctat taagaacaat ttaggggtta acacaaaagc tgagtcagta aaagacaaaa
     3601 aagcgaaagc cattgcagag ctaatgatta gcgtaagaaa tgaatacagc aataaattaa
     3661 caataacatc aataaagaaa tggcataaac tcttattttt aaattctcaa gcaatcaatg
     3721 caggtaaatg gcgttcaggc aaagaaccta tgcaggttgt ttcaggtaga ataggcaaag
     3781 aaatcgtaca ctttgaagct ccaccatctg aaagagtagc taaagaaatg aaacttttta
     3841 ctgaatggta caatgcatat aagattacag acaatatgac aaatgccatt gtaaaatcag
     3901 ctattacaca tttatatttt gaaagcatcc acccttttga agatggcaat ggcagaatag
     3961 gaagagcaat tgctgaaaaa tgtttttcac aatcattagg caggccggtt tatttaagtt
     4021 tgtcaagtat aattgagcga aataaaaagc aatattattt ggcattaaag gaagctcaag
     4081 gctcactgca catcaacaac tggattgttt acttcgcaaa catcatatta caatcacaaa
     4141 aagaagcaat tagaactgca caatttgctc ttaaaaaaat tatttttctt aataatcatg
     4201 ctttaaacct aaatgaacgt gaacagaaag tggtgaataa aatgcttgaa gccggatggg
     4261 atggctttga aggtggtatg acagcaaaaa agtatatgaa aataacaaaa tcttcaaaag
     4321 ctactgccac acgtgactta caacatttaa ctgaacttgg tgtgtttaat tcgaaaggaa
     4381 atggaagaaa tattcattat gaactgacaa tctaagccaa tctcatttct ttactcaaag
     4441 actcaataaa tcaaaaagtt tccaattaaa taaacctaca gctttccaaa ccttttaact
     4501 ttctaaacct tctaaactta cgtcaataaa aaaaccgg
//
LOCUS       DAOOOL010000172         4511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2149_length_4511_cov_75.697038, whole genome shotgun sequence.
ACCESSION   DAOOOL010000172 DAOOOL010000000
VERSION     DAOOOL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_4511_cov_75.697038"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PKU82_10825"
     CDS             <1..1435
                     /locus_tag="PKU82_10825"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83406.1"
                     /translation="YMRIRYTLLSVIFILFTSNAFAWWNDILVYNGKPIAQHSMEAKS
                     NGTLYLAVPDSSAGMYSIQLLKSTNDGNTWLPVSNTTGPMATAITKTKMVHTGSDSIY
                     CLYSTIAGVYLLNVETGVIGSFTYSAVEDFDAAGSPAGNVIYLFVNEPINFTIKRYTT
                     LDGGLTWGGSTATVTTSGQTPRVYMSGTRLILNYYGPVSSDPSTSKIRAATYDESVPG
                     TIGATAFIDIATNTFVKKKQFKSVYNYNTVWFFFTEGDTSQVIKCRISTDNGDNYGPE
                     QIVAGNNGYSNYNFDACHYANFGAWGCYLTYYNSNATLPPTPFPMMFTNASLNTPTVF
                     STPVAYSDFNVVDTPSYHLPVITAFNGTTNDVGVAWVENSGNGSWVYFDRLSAATGMN
                     EVSQDGFISGIIFNAAELNIRFVESPKNKTTIQLSDVTGKIISQTTSVLQENLISVKM
                     PSLTSGTYLLHISDGRHSATKKLILSK"
     gene            complement(1442..2056)
                     /locus_tag="PKU82_10830"
     CDS             complement(1442..2056)
                     /locus_tag="PKU82_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83407.1"
                     /translation="MIVFVGYLASAFLAVSLLMSNAFKFRWYNLAGQIAFIIYGYLIN
                     AMPVIIANVILMIINIYQIIQLYKSKEAFDVIPIKESDPLAERFIERNKKEILEYFPK
                     FEININSGFAIMVFRDMTVANIFIADVAANGDAIVRLNYTIPKYRDYKVGKYLFELEK
                     EFLITNGLKKITYNNLNHAGHIRFINALGFSKGNDAGNYFKILN"
     gene            complement(2183..3223)
                     /locus_tag="PKU82_10835"
     CDS             complement(2183..3223)
                     /locus_tag="PKU82_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOZ83408.1"
                     /translation="MESTATTPTPLFTSKDLVTDQDIRWCPGCGDYSILKQVQTVIPE
                     LGLPREQIVFISGIGCSSRFPYYMETYGMHSIHGRATAIASGLKAARKDLSVWIVTGD
                     GDSMSIGGNHFIHLLRRNFNVNVLLFNNQIYGLTKGQYSPTSESGKVTKSTPFGSIDH
                     PFNPAALAMGADGTFVARSMDRDPVHLKEMLRQAAQHKGTSFLEIYQNCNVFNDGAFE
                     VFTEKASKKQEALFVEHGKPLVFGQNGELGIKLDGFKPVIVNHAEKGISTNDLWIHDM
                     YDRVKSGILARFFDNPANEGHFPRPFGVFYKEERSCYEDDLLQQVEAVISTKGKGDLN
                     KLLRGNEVWEIQ"
     gene            complement(3267..>4511)
                     /locus_tag="PKU82_10840"
     CDS             complement(3267..>4511)
                     /locus_tag="PKU82_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOZ83409.1"
                     /translation="DANIKVLKAGYHYGDTTETFRTRYTVQPAKMKPGVYRNIMGNQA
                     TAIGLVAASQKAGIDLFFGTYPITPASDILHELSKYKSFGIKTFQAEDEIAAVCSAIG
                     ASFGGALGVTSSSGPGIALKGEAMGLATMLEIPLVVINVQRGGPSTGLPTKTEQSDLM
                     QALYGRNGEAPMPIVSASSPADCFNAIYEACRIAIEYMTPVIFLSDGYIANGAEPWQF
                     PTADQLREFKVKYATETNGEHFLPYKRDEKLSRPWAIPGTKGLEHRIGGLEKQHETGN
                     ISYDNDNHQFMVKMREAKVEKVADSIPMQTLDSGPEKGKLLVVGWGSTYGAIKTACQM
                     ARKEGYEVAHAHVTYLKPFPKNFGEMLFKYDKVLMPEMNMGQLSRVVRDKFMIPAIAM
                     NKVMGIPFSATEIKEKIVELMK"
BASE COUNT         1404 a          887 c          767 g         1453 t
ORIGIN      
        1 gtatatgaga atacgttata cattactatc agttatattt attctattca catcaaatgc
       61 atttgcctgg tggaacgaca ttttggttta caatggcaaa cctattgcac aacatagtat
      121 ggaagcaaaa agtaatggta cattatatct tgctgtgcca gacagcagtg caggcatgta
      181 ttccattcaa ttattaaaat caaccaacga tggaaacaca tggcttcctg taagtaatac
      241 aaccgggcca atggcaactg ctattacaaa aactaaaatg gtgcacacag gcagtgatag
      301 tatttattgt ttatatagca ctattgcagg tgtatattta ttgaatgttg aaaccggtgt
      361 tataggctca tttacatatt cggctgttga agattttgat gcagcaggtt caccggcagg
      421 caatgtaatt tatttatttg ttaacgaacc aataaatttc acaataaagc gctacacaac
      481 attagatgga ggtttaacct ggggtggaag tacagcaact gtaacaacgt caggacaaac
      541 gccaagagta tatatgtcag gcactcgatt gatattaaat tattatggtc cagtaagtag
      601 cgatccttct acttctaaaa taagagctgc cacttatgat gaaagcgttc ctggcacaat
      661 aggtgctaca gcttttattg atattgccac taatacattt gtaaaaaaga aacaattcaa
      721 atcggtttat aactataata cagtttggtt tttctttact gaaggcgaca cttcacaagt
      781 aataaaatgt cgaatcagca cagataatgg agacaattat ggacctgaac aaattgttgc
      841 aggcaacaat ggctatagca actacaattt tgatgcctgt cattatgcaa attttggtgc
      901 atggggttgc tatttaactt attataattc caatgccaca ttaccaccta ctccatttcc
      961 aatgatgttt accaatgcct cacttaatac accaactgta tttagcacac cagttgcata
     1021 cagtgatttt aatgttgttg atacaccttc ttatcacttg cctgttatca cagcctttaa
     1081 cggaactacc aatgatgttg gtgtggcatg ggttgaaaac agtggaaacg gaagttgggt
     1141 ttactttgac cgattgagtg ctgcaacagg aatgaacgaa gttagtcaag atggatttat
     1201 ttcaggaatc atctttaatg ctgctgaatt aaatattcgt tttgtagagt caccaaaaaa
     1261 taaaactaca attcaattat cagatgttac cggaaaaata atttctcaaa ctacttcagt
     1321 gttacaagaa aatttaattt ctgtaaagat gccatcatta acatctggaa cttatttact
     1381 tcatattagc gatggcagac attcagcaac aaaaaaactt atattaagta agtaatcaga
     1441 attagttcaa aattttaaaa tagtttcctg catcattgcc tttgctgaag ccaagcgcgt
     1501 taataaaacg aatatgtccg gcatgattaa ggttattata agttatcttt tttaatccat
     1561 ttgtgattaa aaattctttt tctaattcaa ataaatattt tccgacctta taatctctgt
     1621 acttagggat ggtataattg agtcgtacaa ttgcatcacc atttgcagcc acatcggcta
     1681 taaaaatatt ggctactgtc atatcgcgaa aaaccataat ggcaaagccg gaatttatat
     1741 tgatttcaaa ctttggaaaa tattccaata tctctttttt gtttcgttca ataaaacgtt
     1801 ctgctaatgg atcgctttct ttaataggaa tcacatcaaa tgcttctttg cttttgtaaa
     1861 gttgtattat ctgataaata ttgatgatca tcagaataac attagcaata ataacaggca
     1921 ttgcattgat gagataccca tatattataa atgcaatttg tcctgccagg ttataccatc
     1981 tgaatttaaa cgcattgctc atcagcaatg aaacagccaa aaatgctgat gccagatatc
     2041 ctacaaatac aatcacagaa tcaaatttaa gttataaaaa caattgttgc ttaaaaaccg
     2101 gttacgtata gccactacaa ctgttatcat gaaataaaaa aataacagtt taaaattatg
     2161 gcaataaaat attattcagg atctattgaa tttcccaaac ttcatttcca cgcaaaagtt
     2221 tattcaaatc acctttgcct tttgttgaaa taactgcttc aacctgttgt agtaaatcat
     2281 cttcatagca acttctttct tctttataga agactccaaa cgggcgtggg aagtggcctt
     2341 cgtttgcagg gttatcaaag aacctcgcta aaattcctga cttaactcta tcgtacatgt
     2401 catgaatcca aaggtcgttt gtgctgatac ctttttctgc atgattaaca atgacaggtt
     2461 taaatccatc aagcttaatt cccaattcac cattctgccc gaacaccaat ggtttgccat
     2521 gttcaacaaa gagtgcttct tgttttttgg atgctttctc tgtaaatact tcaaatgcac
     2581 catcattaaa tacgttacag ttctgataaa tctcaagaaa ggaagttcct ttatgctgtg
     2641 ctgcctgacg gagcatttct tttaaatgca caggatcgcg atccatgctt cgtgccacaa
     2701 aagttccatc ggcacccata gctaaagcag caggattaaa aggatgatca attgaaccga
     2761 aaggtgttga cttagtcact tttcctgatt ctgatgttgg cgaatactga ccttttgtaa
     2821 gtccgtatat ctgattgtta aacaacagaa cgtttacatt aaaatttctg cgcaacagat
     2881 gtataaagtg gtttccacca atagacattg aatcgccatc acctgttaca atccaaacac
     2941 taaggtcttt gcgtgcagct ttgagtccac ttgctatggc tgttgctctt ccgtgaatag
     3001 agtgcatacc atacgtctcc atgtaatatg ggaaacgtga agagcatccg attcctgaaa
     3061 taaaaacaat ctgttcgcgt ggcagaccca actcaggaat aacggtctgt acctgcttta
     3121 atattgaata atcgccacat cccggacacc aacgaatatc ctgatcggtt accaaatctt
     3181 ttgatgtaaa taacggtgtg ggtgttgttg cagttgattc cataaaaata ttatctatta
     3241 aaaactgatt taaatttttc ttttggttat ttcattaatt caacaatctt ttcttttatc
     3301 tctgttgctg aaaaaggtat tcccataact ttattcattg ctatggcagg tatcataaac
     3361 ttatccctca ccactcttga aagttgtccc atattcattt caggcatcag cactttgtca
     3421 tatttgaata acatttcacc aaaatttttt ggaaatggtt tcagataagt aacatgagca
     3481 tgtgctactt cataaccttc tttgcgggcc atctgacaag ctgttttaat agcaccatac
     3541 gtacttcccc aaccaacaac gagtaatttt cctttttcag gaccgctatc taatgtttgc
     3601 attggaatac tatcggctac tttttccact ttagcttcac gcattttcac cataaactga
     3661 tggttgtcgt tatcgtaact gatgttgcct gtttcatgtt gtttttcaag accgccaatt
     3721 cgatgttcta acccttttgt tccgggaatt gcccatggac gagaaagttt ttcatcacgt
     3781 ttataaggaa ggaagtgttc tccatttgtt tcagttgcat atttcacttt aaattctctt
     3841 agctgatcgg cagttggaaa ttgccaaggc tccgcaccat ttgcaatata accatcagaa
     3901 agaaaaataa caggcgtcat atattcaata gcaatgcggc aggcctcata aattgcatta
     3961 aagcaatcag cgggtgaaga agctgaaaca attggcattg gtgcttctcc atttctgcca
     4021 taaagcgcct gcattaagtc actctgttct gtcttagttg gcaatcctgt tgaagggcct
     4081 ccacgctgaa catttataac caccaatgga atttcgagca tagtagctaa gcccattgct
     4141 tcacctttca aagcaatacc cggacccgat gatgatgtta cacccaaggc accaccaaat
     4201 gaagcaccaa ttgctgaaca aactgctgca atttcatctt ctgcctgaaa agttttaatt
     4261 ccaaaacttt tgtacttaga aagctcgtgc aatatgtctg atgcaggagt aattggatag
     4321 gttccaaaaa acaaatctat tcctgctttt tgtgatgccg caaccaaacc aattgctgtt
     4381 gcctgatttc ccatgatgtt tctatataca ccgggtttca tttttgcagg ctgtacagta
     4441 tatctggtgc ggaacgtttc tgttgtatca ccatagtgat aacctgcctt caacacttta
     4501 atgtttgcat c
//
LOCUS       DAOOOL010000173         4503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2152_length_4503_cov_30.270234, whole genome shotgun sequence.
ACCESSION   DAOOOL010000173 DAOOOL010000000
VERSION     DAOOOL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_4503_cov_30.270234"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKU82_10845"
     CDS             <1..357
                     /locus_tag="PKU82_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83410.1"
                     /translation="VLVFPGEGGAGRVVTLTNENTTLIEGLALAGGISQNGIAKKIKL
                     IRGDTRNPQVTIIDLSTIEGMKESNLLLQANDIIYVEPRKRISQGILSELAPIVAIFS
                     GIASIFIAYDVIKRNQ"
     gene            364..2796
                     /locus_tag="PKU82_10850"
     CDS             364..2796
                     /locus_tag="PKU82_10850"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOZ83411.1"
                     /translation="MPKKQITPLNEEFDFKLFVTIAQRNVEWFLMFMTLSIIVWYVIV
                     RYTPPMYESAALMKLNTDNNVTKALNITRDVYQEQNNEVAGNLELIRSSIIVGKAIAT
                     LPMRVTYISKGTLLENEMYKSCPFEVNFLVKDSLWMDRPFYLQFNNEKTFVLSYTPVG
                     QRDQVDEEYEINKWIHLNGISLQINIIDFQRILKYQSQLKKNTFKFIINSDKALIKDF
                     SSKLSVIMANPDAKTINIKIKEKNATKAADMSNAISQEFLKFDVLRSREATDRILEFI
                     DTTLYRVNNDLSESEMNLERFKSNNRIITPELLASDLSDKMSQLELRLTDYTLNLLAI
                     SELKKTIESEKKVDEKMLSLTGIYKTDEIKDVLSNLNILLDKREQALIMVTDKAEYVQ
                     MLSGRIQRQKDYLLQSVAYEENGIKQKKAELESQYRKLNSNYLGIPEQQTEFERLQRI
                     FNVNEKFYSLLLEKKAEFSITRAGYVSKNIILEAARPAPAPYYPNKSLILSSCIMVGF
                     VASFLLIFIKYLFYNQIGSLEEIAQYTDAPLLGIVPRYKREIPISQLLVDKSPKSVIA
                     EAFRSIRTNLQFINNDDKPQLMALTSTISGEGKTFNAINLAGVISFSGKKVIILDLDM
                     RKPKIHLGFNVENNRGMSTILIGKDTIENCINHSALENLDFITAGPIPPNPSELIISP
                     KMDELLEYLKVRYEVIIADTPPVGVVSDGIPMIQKADFPIYILRANYSRKMFIHNINK
                     IISENKVPRLSVVLNSVDQTKLKYGYGYGYGYGYGYGYGYSYGYGYGYYSEDDKKPTG
                     FWNRIISFFIRK"
     gene            2803..3345
                     /gene="rfbC"
                     /locus_tag="PKU82_10855"
     CDS             2803..3345
                     /gene="rfbC"
                     /locus_tag="PKU82_10855"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOZ83412.1"
                     /translation="MKIIKTALNGICILQADVFSDERGYFLESYHKEKLKNLGIEAEF
                     YQDNFSLSAKNVLRGLHFQNPPFDQGKLIKVISGSVLDVVVDIRKSSATYGKNFTIEL
                     NSEDHLMVWIPPGFAHGFVSLAENTLFYYKCTKPYNKDSEAGIIYNDPALNINWGVNQ
                     PVLSEKDKILPNFSDATILF"
     gene            3445..>4503
                     /locus_tag="PKU82_10860"
     CDS             3445..>4503
                     /locus_tag="PKU82_10860"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOZ83413.1"
                     /translation="MKDIVNHNYHLFIYAVLFLGSLAFSFLINSLFLRFFFTLGIRNT
                     NDGTVIRWGSMSKPAVGGLSFYIVFLLSLATYSVFFNNNDALYNSQFMGFIMAMAIGF
                     LIGLADDAYNTKPVLKFLAQVSCAFSFIFTGTYIDLFDNMILNYLLTIFWVVGIMNSV
                     NMLDNMDGITSTVSLGVIISAVVVITMSNDINNLYLASIIGLSAGLLGFLYFNWHPSK
                     MYMGDTGSQFLGVFLAGIGIMFFWNNPYHITESLPIKKILTVAIAFLLPLVDTTVVVV
                     NRISKGKSPFIGGKDHTTHSLAYLGLKDNQVAWVYAAISLISVILVYVLNFLITTWDI
                     LYTVIFAGYFLIVLAVFFY"
BASE COUNT         1477 a          717 c          812 g         1497 t
ORIGIN      
        1 gtattggttt ttccaggaga aggtggtgcc ggtagagtag ttacattaac caatgagaac
       61 acaactctta ttgaaggact tgcacttgca ggtggtattt cacaaaatgg tattgcaaaa
      121 aagataaaat taatcagagg tgatacaaga aacccacagg taacaattat tgacctttca
      181 acaattgaag gcatgaaaga atctaacctt ttattgcagg ctaatgatat tatttatgtt
      241 gagccaagaa aaagaatttc acagggaatc ttgtctgaac ttgctccaat cgtagctatc
      301 tttagtggaa ttgcaagtat ctttattgct tatgacgtaa ttaaaagaaa tcaataatcg
      361 ctgatgccta aaaaacaaat aacccctcta aacgaggaat ttgattttaa gctttttgta
      421 accattgctc agcgcaatgt ggagtggttc ttaatgttta tgacactttc cataattgtt
      481 tggtatgtca tagttcgtta tacaccgcca atgtatgaat cggcagcatt gatgaagtta
      541 aataccgata acaatgttac caaggcatta aatattacgc gcgatgttta tcaggaacaa
      601 aacaatgaag tagccggtaa ccttgaactc attcgttcaa gtataattgt tggtaaagca
      661 atagccacat tgcctatgag ggtaacctac atttccaaag gcacattgct tgaaaacgaa
      721 atgtataaat cgtgtccatt tgaagtaaac tttttggtaa aagactcttt atggatggac
      781 cggccatttt accttcagtt taacaatgag aagacatttg ttttgtctta tacacctgtt
      841 gggcaaagag accaagttga tgaagaatat gaaattaata aatggattca tctgaatgga
      901 atttcacttc aaataaatat tattgatttt cagcgaatat taaaatatca atcacaacta
      961 aaaaagaaca cttttaagtt tattattaat tccgataagg cacttattaa ggatttcagt
     1021 tctaagcttt cagtcatcat ggctaatcca gatgccaaga caataaatat caaaatcaaa
     1081 gaaaaaaatg ctactaaggc agctgatatg tccaatgcca tcagccaaga gtttttgaag
     1141 tttgatgtat tgagaagcag agaagctaca gaccgtattc ttgaatttat tgatactaca
     1201 ctttacagag taaataatga tctctccgag tcagagatga atctcgaacg atttaaaagt
     1261 aataacagaa tcataactcc ggaattgctt gcatctgact taagtgataa gatgagtcag
     1321 ctggagctca gattaacaga ttatacttta aatctacttg ctatatctga acttaaaaaa
     1381 acgatagaga gtgagaagaa agttgatgaa aaaatgctca gtttaaccgg aatttataaa
     1441 accgatgaga taaaggacgt gctaagtaat ttaaatattt tacttgataa acgcgaacaa
     1501 gctttgataa tggttactga taaagcagaa tatgttcaaa tgctttccgg aagaattcaa
     1561 agacagaaag attatttact gcagtctgta gcttatgagg aaaatggcat taagcagaaa
     1621 aaagcagaac tggaatcgca atataggaaa ctgaacagta attatttggg tattcctgag
     1681 caacaaacag aatttgaacg gttacagcga attttcaatg taaatgaaaa gttctattca
     1741 cttttacttg aaaagaaggc agagttttca ataactcgtg ccggatatgt ttctaaaaat
     1801 attattcttg aagcggcacg tcctgcacct gcaccatatt atcctaataa aagcctgata
     1861 cttagttcat gtattatggt tgggtttgtt gcaagttttt tattgatatt tattaaatat
     1921 ctgttttata atcaaattgg gtctcttgaa gaaattgcgc aatataccga tgcgccatta
     1981 ttaggaattg taccaagata taaaagagaa attcctatct cgcaattatt ggttgacaaa
     2041 agtccaaaat ctgtgatagc agaagctttt cgttctattc gaacgaatct tcagtttatc
     2101 aataatgatg ataagccgca attaatggcg ctgacatcaa ccatttcagg tgagggaaaa
     2161 acatttaatg caattaacct cgcaggtgtt atttcatttt ccggaaagaa agtaatcatt
     2221 ctcgacttgg atatgcgtaa gcctaaaatc cacctgggat ttaatgtgga aaacaacaga
     2281 ggaatgagta ctatactaat tggtaaggac accattgaaa actgtattaa tcacagtgca
     2341 ctcgaaaatc ttgattttat cactgcggga cctattccac caaatccatc tgagttgatt
     2401 attagcccta aaatggatga attgcttgaa tatttgaaag tacggtatga agtaattatt
     2461 gccgatacac ctcctgttgg tgttgttagt gatggaattc ctatgattca gaaggcagat
     2521 tttccaatat atattcttcg tgcaaattac agtcgtaaaa tgtttattca taacatcaat
     2581 aaaattattt ctgaaaataa agtaccacga ctttctgtag ttttaaatag cgttgatcaa
     2641 accaaactca agtatggata tggttatggt tacggctatg gctatggtta tggctacgga
     2701 tacagttatg gctacggcta tggttattat tccgaagatg ataaaaagcc aacaggcttc
     2761 tggaacagaa ttattagttt ttttattagg aaataacctc caatgaaaat tattaaaact
     2821 gctttaaacg gcatttgtat tttgcaggct gatgtttttt cagatgagcg gggatatttc
     2881 ttggaatcat atcataaaga gaaattaaaa aaccttggta tagaagcaga attttatcag
     2941 gacaactttt cattgtcagc caaaaatgta cttcgcggcc tgcattttca aaaccctcca
     3001 tttgatcagg gtaaactgat aaaagttatt tccggttctg tgttagatgt tgtagttgat
     3061 attcgaaaat cttctgctac ttatggaaag aattttacaa tagaattgaa ttcagaagat
     3121 catttgatgg tgtggattcc accgggtttt gcgcatggtt ttgtttcttt agcagaaaac
     3181 accctttttt attataaatg cacgaagcca tataataaag attctgaagc aggaataatt
     3241 tataatgacc ctgcattaaa tattaattgg ggtgtaaatc agccggtttt atcagaaaaa
     3301 gataaaattt taccaaattt tagtgatgca actattttgt tttaaatttc tatactatac
     3361 ttttgctcta attaagtata acaataaccc caccaacaca tctcatgcag gcgtagcatc
     3421 aatggttggt ttaattcaaa caaaatgaaa gacattgtaa accacaacta tcatttattt
     3481 atttatgccg tattgttttt aggatcactg gcattttcat ttttaattaa ctcattgttt
     3541 ttacgtttct tttttacttt aggaattcga aatacaaatg acggaactgt aatccgatgg
     3601 ggctccatgt ccaagcctgc cgtaggtgga ttgtcattct atattgtatt cttgctttcg
     3661 ttggcaacat actctgtttt ttttaataat aatgatgcat tatacaactc tcagtttatg
     3721 gggtttatca tggcaatggc aattgggttc ctgattggcc ttgctgacga tgcttataac
     3781 acaaaacctg tacttaaatt tcttgctcag gtatcatgcg catttagttt tatttttacc
     3841 ggcacctata ttgacctttt cgataacatg attctcaatt atcttttaac aatattctgg
     3901 gtggttggaa tcatgaattc tgtaaatatg ctcgataata tggatggtat tacttcaact
     3961 gtcagtttgg gtgtaataat ttctgcagtt gtagtcatca caatgagtaa tgatattaat
     4021 aacctttatc ttgcttcaat catcggactt tcagcaggac tgctaggctt tttatatttt
     4081 aactggcatc cttcaaaaat gtatatgggc gacacaggca gtcagtttct tggagtattt
     4141 cttgccggta taggaatcat gtttttctgg aacaatccat atcatataac agagtcgctt
     4201 cctatcaaaa aaattctgac tgtagccatt gcatttttac ttcctttagt tgataccact
     4261 gttgttgttg taaacagaat cagtaaaggt aaaagtccct tcataggagg taaagatcat
     4321 acaactcata gtttagccta tctgggatta aaagataatc aggtagcttg ggtttatgct
     4381 gcaatttcat taatatctgt aatcttagtt tatgtgttaa atttcctgat aaccacttgg
     4441 gatattttat ataccgtaat ttttgccgga tattttctta tcgttctggc tgtgtttttt
     4501 tat
//
LOCUS       DAOOOL010000174         4483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2164_length_4483_cov_38.483966, whole genome shotgun sequence.
ACCESSION   DAOOOL010000174 DAOOOL010000000
VERSION     DAOOOL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_4483_cov_38.483966"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..652)
                     /locus_tag="PKU82_10865"
     CDS             complement(248..652)
                     /locus_tag="PKU82_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOZ83414.1"
                     /translation="MTTINHVPIVIGTGSTPIAIGAGGGAKPKVKPSAFLFITMVYSV
                     YILYSIKLGVHYIGTTNDVARRLEQHNKQEFADSFSAKGIPWELKFVIGELNSTQAFA
                     IEKHIKKMKSVVYIENLIRFPEIVEKLKHKYK"
     gene            complement(722..795)
                     /locus_tag="PKU82_10870"
     tRNA            complement(722..795)
                     /locus_tag="PKU82_10870"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(759..761),aa:Asn,seq:gtt)
     gene            complement(959..2497)
                     /locus_tag="PKU82_10875"
     CDS             complement(959..2497)
                     /locus_tag="PKU82_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOZ83415.1"
                     /translation="MLVKTYGCAVYGINATPITVEVNVDQGTKFFHVGLPDNAVKESH
                     QRIEAALKNTGFKMPGKKVVVNMAPADIRKEGSAYDLTIAMGILSASQQIVAENIEQY
                     IIMGELSLDGSLQPIKGALPIAIEARKHGFKGFFLPQQNAREAAIVDSLDVYGISNIK
                     EVIDFFTGEGTLQPVVINTRDEFYSAQTKSEFDFCDVKGQENIKRSLEIAAAGGHNVI
                     LIGPPGAGKTMLAKRLPTILPPLTLHEALETTKIHSVAGKMGKQASLISIRPFRSPHH
                     TISDVALVGGGGIPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEERRVTISRAK
                     FSVEYPASFMLVASMNPCPCGFFNHPEKECVCAPGIVQRYLSKISGPLLDRIDLHVEV
                     TPVSFSELSKKRVSEKSEDIRKRVVEARVIQEERYKEQEGVYCNAQMSSKMLHDICRI
                     SDEGNQLLKTAMEKLGLSARAYDRILKVSRTIADLAASADIKTEHLAEAIHFRSLDRE
                     GWAG"
     gene            complement(2612..3028)
                     /locus_tag="PKU82_10880"
     CDS             complement(2612..3028)
                     /locus_tag="PKU82_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HOZ83416.1"
                     /translation="MPYPEQLCAPMRQELTNVGFEELKTTSDVESSLPNSKGTVLLVV
                     NSVCGCAAGTARPGVTLSLRGDKKPGKLVTVFAGQDVEATAQARKYTLPYPPSSPCIA
                     LFKDGELVHFVERHHIEGRSAEMIAENLKAAYEEFC"
     gene            3307..3660
                     /locus_tag="PKU82_10885"
     CDS             3307..3660
                     /locus_tag="PKU82_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83417.1"
                     /translation="MAEHKTSTSKYENNMFPVIKLDYSGKIIYHNVAAMPILSYWQCK
                     LNAPVNNQVKAIYPEIFNVKTPVPHDASVQYLEYLFHFSVIPYPEAGYIGIYGHCIEE
                     ALVAVTNSTEQHHSK"
     gene            3657..4178
                     /locus_tag="PKU82_10890"
     CDS             3657..4178
                     /locus_tag="PKU82_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOZ83418.1"
                     /translation="MIRTVTAIPEGNFNSVDFSEVIRQSIDRLRYTEGIDKVNFDIKV
                     DMKAPFAGNNKLLEVVFNNLISNGIKYRKITGDPSSMRLHVYCDEQKAIINIQDNGLG
                     IAPDEVGQIFNLFQKEKQPEPGRGIGLYMAKEIIEKLGGNITVESVLNTGTAFIIEIP
                     NKLNTKPLNNYFE"
     gene            4183..>4483
                     /locus_tag="PKU82_10895"
     CDS             4183..>4483
                     /locus_tag="PKU82_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ83419.1"
                     /translation="MANKYHSVLVVDDSEAEQVMIKQIITSEPFCDQLNVYADARKAL
                     DFIKATPIDQLPEIIFLDIVMPEMDGFQFLNEFEKLSESVRQKCKIVLLSSSDSFK"
BASE COUNT         1384 a          905 c          778 g         1416 t
ORIGIN      
        1 aaatgagata aaaaatagtt tgggattttt ttatcagaat aagagaatga ataaatgtta
       61 gcttgattca atgttatgct attctaatct tcttaaaaca tcattagttg ttccaatata
      121 atgtacacca agttttattg agtaaagaat ataaacagaa taaaccatag taataaataa
      181 aaaagccgaa ggctttacct tcggctttgc tccccctcct gtcccgatag ctatcgtggt
      241 cgaaccatta tttatattta tgtttaagct tttcaacaat ctccggaaac cgaatcagat
      301 tttcaatgta cacaacagat ttcatcttct taatatgttt ttcaattgca aaagcttgcg
      361 tactatttaa ttctccaata acaaatttta attcccatgg aatccctttt gctgaaaacg
      421 agtctgcaaa ctcttgcttg ttatgctgtt ctaatcttct tgcaacatca ttagttgttc
      481 caatataatg tacaccaagt tttattgagt aaagaatata aacagaataa accatagtaa
      541 taaataaaaa agccgaaggc tttaccttcg gctttgctcc ccctcctgcc ccgatagcta
      601 tcggggtcga accagttccg attactattg gaacatgatt aatagttgtc atttcttcaa
      661 agtacttact gattttttta aatcaaaatt aaaaaagccg aaggctttac cttcggcttt
      721 gctccccctc ctgggctcga accagggacc ccatgattaa cagtcatgtg ctctaaccag
      781 ctgagctaag gaggaattta cctcttaaga atttctgccc taagacagaa aaatgttttt
      841 cttaactcta accattccga taacaatcgg aactaaagca cgtgtaatca cgaacacgtt
      901 tgtaaaggga cggcaaaaat agcttttttt ggttaaaaaa aaattttaac cctatttttt
      961 atcctgccca gccttctcta tctaaacttc tgaagtgtat tgcctcggcc aaatgctcgg
     1021 tttttatatc agcactggca gctaaatcgg ctatagttcg agatactttt aatattctgt
     1081 cataggctcg tgccgaaaga cctaatttct ccattgcagt ctttagcagt tgattaccct
     1141 catcagaaat acgacagatg tcgtgcagca tcttactgct catctgtgca ttacagtaaa
     1201 cgccttcctg ctctttatat ctttcttcct gaattacccg tgcttcaaca accctttttc
     1261 tgatatcttc gcttttttcg gagacacgtt ttttcgatag ctctgaaaaa gaaaccggtg
     1321 taacttcaac atgtaggtca atcctgtcga gcaatggccc tgaaatttta ctgagatagc
     1381 gctgcacaat acccggagca caaacacatt ctttttccgg atgattaaaa aatccacaag
     1441 gacaggggtt catagaagct acaagcataa aactggcagg gtattctaca gaaaatttag
     1501 ctctggaaat ggtcaccctg cgttcctcca gcggctgacg catcacttca agtactgttc
     1561 gtttaaattc aggcaactcg tctaaaaaca atacgccatt atgtgctagt gatatttctc
     1621 ctggctgtgg aattccgcct ccgccaacta atgcaacatc gcttatggta tgatgaggtg
     1681 accggaacgg acgtatagaa attaatgatg cctgtttacc cattttgccg gcaacggaat
     1741 ggatttttgt tgtttctaat gcctcatgta atgtcaatgg tggcaatatg gtaggaagtc
     1801 gctttgccag catggtctta cccgcaccgg gcggaccaat caatattaca ttgtgccctc
     1861 ctgctgctgc aatttccaga gagcgtttaa tgttttcctg acctttaaca tcacagaaat
     1921 caaactcact ttttgtctga gcagaataaa actcatcgcg tgtattgatc acaacgggtt
     1981 gcaaagtgcc ttcaccggtg aaaaaatcta taacctcttt aatgttactg ataccataca
     2041 catcgagcga atctacaatg gccgcctcgc gtgcattttg ttgcggcaga aaaaaacctt
     2101 tgaatccatg tttgcgcgct tcaatagcta ttggcaatgc cccttttata ggctgcaggc
     2161 tgccatcgag cgaaagctca cccatgataa tatattgctc aatgttttcg gcaactatct
     2221 gctgacttgc agaaagtatt cccatagcaa tagttaaatc ataagccgag ccttctttgc
     2281 gaatatccgc tggggccatg tttactacta ccttttttcc gggcatttta aagcctgtat
     2341 ttttcaatgc tgcttcaatt cgctgatggc tttcttttac agcattatcg ggtaagccaa
     2401 catgaaaaaa tttggttccc tgatcaacat ttacttctac cgttattggt gtggcattga
     2461 ttccatagac tgcacagccg taagttttaa caagcatata aaataataat ggctaattct
     2521 gtttttctat tcgataaaca aaaatagcca ttaataactt taaaaaccta aaacatcatc
     2581 cgcaaaggtt ttttttctgc ggaaaatgtg gctaacaaaa ttcttcgtaa gcagctttca
     2641 gattctctgc aatcatttca gccgagcggc cttcaatatg atgacgttct acaaaatgta
     2701 ccaactcacc gtctttaaac aatgcaatgc atggtgaaga tggcggatat ggcagggtgt
     2761 attttctggc ttgtgccgtt gcttcaacat cttgtcctgc aaatactgta accaattttc
     2821 cgggtttttt atcacctctt aaagaaagtg ttactcccgg acgtgccgtt cctgcagcgc
     2881 atccgcatac tgagtttaca accaacagca ctgttccttt gctgtttggt aatgatgatt
     2941 caacatctga agtggttttt aattcctcaa aacctacatt ggttaattcc tggcgcatag
     3001 gtgcgcataa ttgttccgga tatggcattt ttaaatattt ttttgcaaaa gtatataaat
     3061 actcttattt gttattcagt tcagataaca gattttaaag cacattgttt aagtttctta
     3121 caatattttt tgacaccaca gaattcctga attttttctt ttctcatggg caattcaatt
     3181 ttaattgaat aaaaagttga ataatttaag caaaatctta catctgtaag cataaccacc
     3241 catagcagtg aagcatttct gaaaaactgt cgttaaacta ctgtttaatt taaagaaatc
     3301 tccacaatgg cagaacacaa aacctctact tcaaagtacg aaaacaacat gtttcctgta
     3361 ataaaattag attacagcgg aaaaattatt tatcacaatg tagcggcaat gcctatacta
     3421 agttattggc aatgcaaact aaatgctcct gtaaacaatc aggtaaaagc tatttatcct
     3481 gaaattttta atgtaaaaac gcctgtgcca catgatgctt cagtgcaata tcttgaatac
     3541 ctatttcatt tctctgtaat tccatatccc gaagcaggat atatcggaat ttatggacac
     3601 tgtattgaag aagcattagt tgctgtaaca aattcaacag agcaacatca ttcaaaatga
     3661 tcagaacggt tactgcaatt cccgaaggca attttaattc tgttgacttt agcgaagtta
     3721 ttcgtcagtc aatagacagg ttgcgctata cagaaggaat tgataaagta aattttgaca
     3781 ttaaagtaga tatgaaagcc ccttttgctg gcaacaataa attgcttgaa gttgttttca
     3841 ataatttaat ttcaaacggc attaaataca gaaaaattac gggtgaccca tcttctatgc
     3901 ggttacatgt ttattgtgat gaacaaaaag ccatcatcaa tattcaggat aacggactgg
     3961 gaattgcacc tgatgaagta ggacaaattt ttaatctgtt tcagaaagaa aaacagcctg
     4021 aacccggaag aggaattggc ttgtatatgg ccaaagaaat tattgaaaaa ctaggtggaa
     4081 acatcaccgt tgaatctgtt ttaaataccg gaacagcatt catcattgag ataccgaata
     4141 aattaaacac caaaccatta aataactatt ttgaataagc ttatggccaa taaataccac
     4201 tctgttttag ttgtagatga tagtgaagcc gaacaggtga tgataaaaca aatcattaca
     4261 tcggagcctt tttgcgatca gctaaatgtt tatgctgatg cccgtaaagc acttgatttt
     4321 attaaagcaa cacctataga tcagttgccc gaaataattt tcttagatat agttatgcct
     4381 gaaatggatg gctttcagtt tctgaatgag ttcgagaaac tttctgaatc ggtgcgtcag
     4441 aaatgcaaga ttgttttatt gtcgagttca gacagtttta aag
//
LOCUS       DAOOOL010000175         4455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2181_length_4455_cov_42.650227, whole genome shotgun sequence.
ACCESSION   DAOOOL010000175 DAOOOL010000000
VERSION     DAOOOL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_4455_cov_42.650227"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PKU82_10900"
     CDS             <1..407
                     /locus_tag="PKU82_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ83420.1"
                     /translation="QQNMYVTSEDGRIEYVAALPQIVEDEILKLFEDIDLLIKTNLSL
                     GKVFYYAAMIHLVFVKIHPWNDGNGRSARLIEKWFLSEKLGAKAWFMQSEKMYYSRHS
                     EYYNCLRQLGLEYENLNYQKALPFVLMLPDCV"
     gene            514..1062
                     /locus_tag="PKU82_10905"
     CDS             514..1062
                     /locus_tag="PKU82_10905"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOZ83421.1"
                     /translation="MKRSDFIKNLIGIGSSALVLPSYAWIKQYDKFYLLQNFVRGFRY
                     YCGPQLLHQMKEGDMLELVREPANEYDSFAIALHFNKQKIGYVPAESNEVLSKLLDIQ
                     LIELTAEITHLKEEAAAWENVAVAIYVLKEKKPGDILSIEAKELTVLETPDYYSIKNN
                     GERITRFYLDEFDAHDYSTVKL"
     gene            1393..2313
                     /locus_tag="PKU82_10910"
     CDS             1393..2313
                     /locus_tag="PKU82_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HOZ83422.1"
                     /translation="MENNPLLCNPDSGICEMPTNKATATQDGKIKSEKRPIKIVYYTD
                     PICSSCWGIEPQLRKLKLEYSSNIEVEYRMGGLLPDWSYNSGGISKPSDVAHHWDEVS
                     VYYDMPIDGDVWLEDPLQSSYPPSIAFKAAQMQDQDKAILFLREIREMVFLKKKNIAK
                     WEHLESAAKSVGLDVGKFREDYNGKAKELFNEDLALGKQLGVRGFPTMFFTDTSGKTE
                     TVYGSKPYAAFENAIIKLQPDVQKFNYDKSVAAVFANYHSLTAKEYSELTGTARQQSE
                     QVLNDLVANGTLEKLTTKNGAIWKLKNAGQ"
     gene            complement(2461..4212)
                     /gene="rho"
                     /locus_tag="PKU82_10915"
     CDS             complement(2461..4212)
                     /gene="rho"
                     /locus_tag="PKU82_10915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOZ83423.1"
                     /translation="MYDILELSDKLVAELRDIAKELNVENYEGLKKQDLIYKILDHQA
                     IHPELAKSKKADKPKDEKPRHEKPRHEKKEQKPAAAPTVTEKPLEEPASVIATSEVSA
                     EAKTQDDADKPRRKRIIPQGAPAKNELPQPNVIKAPEVTSAPQEAETPARPARVHTED
                     RPQQQPQQQQQQQNNNNNNQNQQNDFQNRNRERRFPDNQQQNNFRQQNDYFEFDNAIT
                     AEGVLEIMPEGYGFLRSSDYDYLSSPDDVYVGQSQIKAFALKTGDTVKCKVRPPREGE
                     KFFPLTKVELINGLEPTVVRDRIPFDYLTPLFPFEKLNLAGENSTMSTRIMDMFSPIG
                     KGQRGLIVAQPKTGKTVLLKEVANAIAANHPEVYLIVLLIDERPEEVTDMARSVKAEV
                     ISSTFDEPADKHVKIANLVLEKAKRLVECGHDVVIVLDSITRLARAYNTTAPASGKIL
                     SGGVDSNALHKPKRFFGAARKIEKGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEL
                     QLDRKLSNKRVYPAIDLVASSTRREDLLLDRKMLERMWILRNHLADMNPMEAMEFLLD
                     RLRGTKSNEEFLISMNS"
BASE COUNT         1363 a          787 c          855 g         1450 t
ORIGIN      
        1 ggcaacaaaa tatgtacgta acctctgaag acgggcgcat agagtatgta gctgcattac
       61 ctcaaattgt agaggatgaa atattaaaac tttttgaaga cattgacttg ttgattaaaa
      121 ccaatttatc tttagggaaa gtattttatt atgctgctat gatacattta gtctttgtaa
      181 aaatacaccc ctggaatgac ggcaatggca ggagcgcccg gctgattgaa aaatggtttt
      241 tatcagagaa gttgggtgca aaggcttggt ttatgcagag tgaaaaaatg tattattcac
      301 gacacagtga gtattataat tgccttagac aacttggttt ggagtatgaa aatctgaatt
      361 accaaaaagc acttcctttt gtattaatgc tcccggattg tgtctgattt aaataactcc
      421 tgaactgcat ttagaattat ttaatagggt ctttttatgt tttaagaaat gatgaattct
      481 cagatttata attgaaattg tattttcgta gttatgaaac gcagcgactt tattaaaaat
      541 ctaattggca ttggatcttc ggcattggta ctaccatctt atgcgtggat taagcaatac
      601 gataaatttt atttacttca aaactttgtc cgagggttca gatattattg tggcccgcaa
      661 ttattgcatc aaatgaaaga aggcgatatg cttgaactgg tacgtgagcc tgcaaatgaa
      721 tacgacagtt ttgccattgc tttgcatttc aacaaacaaa aaattggcta tgtgcctgca
      781 gagagtaatg aagttttaag caagctcctc gatatacaac ttatagaatt gacagcagaa
      841 ataactcatc ttaaagaaga agctgcagcc tgggagaatg tagcggtggc tatttatgtt
      901 ttgaaagaaa aaaaacctgg cgatattttg tcaatagagg ctaaggaact caccgtgttg
      961 gaaacacctg attattattc aataaaaaat aacggtgaaa gaattacacg attttattta
     1021 gacgagtttg atgcgcatga ttatagtacg gttaaactct gaaaacaata aaagactttt
     1081 ttcctgattt gaaaggattt gttttttata aaacacgaat caactatcct gtttttcatc
     1141 ccttactttt ttaatctaca attcattaaa tcaagaaata tataattctc caattctcca
     1201 agactccaac tttccaaact tttaaacttt gcaactttct aaactcatta ccccattgca
     1261 aagtatgtat tttgttctga cctttgcact aagattaaaa atagtaatag gcaatagagt
     1321 ataatgaatt ggacttattt aacagtattt atattcggca ataccaactg cttagcacaa
     1381 acaaaaaaga aaatggaaaa caatccttta ttatgcaatc ctgatagtgg tatttgcgaa
     1441 atgccaacaa acaaggcaac tgcaacacaa gacggcaaaa taaaatcgga aaaaaggcca
     1501 ataaaaattg tttactatac agatccgatt tgctcttctt gttggggtat tgagccacaa
     1561 ttacgcaaat taaaattgga atacagcagc aatattgaag ttgaatacag aatgggtggg
     1621 ttactgcccg attggagcta caatagcggg ggcataagta agccatctga tgttgcccat
     1681 cactgggatg aggtaagtgt ttattatgat atgcctattg atggtgatgt ttggttagaa
     1741 gatcctttgc aatcctcata tcctccttcc atagcattta aagctgctca gatgcaagat
     1801 caggataagg ctattctgtt tttaagagag ataagagaaa tggtttttct gaagaaaaag
     1861 aacattgcaa agtgggagca tttagagtcg gcagctaagt cggttggatt agatgtaggt
     1921 aagtttaggg aggattacaa tggaaaagct aaagagcttt ttaatgaaga cttagcatta
     1981 ggcaagcagt taggtgtaag aggttttccg actatgtttt ttacagatac atcaggaaag
     2041 acagaaactg tatatggttc aaaaccttat gctgcttttg aaaatgcaat tattaaactt
     2101 cagcctgatg tacaaaaatt caactatgat aaaagtgtgg cagcagtttt tgcaaactat
     2161 cattcattaa ctgctaaaga atattcggaa ttaacaggaa cggcaagaca gcaaagcgaa
     2221 caagtcctta atgatttggt tgcaaatgga acattagaaa aactgacaac taaaaacgga
     2281 gcaatttgga aattgaaaaa tgcagggcaa tagtaagtgg gatattaatt gtctaaacta
     2341 tggtgcaaca ctcatgtaac agtaaaaaga gatacatttt tgcattccac attcttcaaa
     2401 tctctaattc tccaattcca caaccttcca aaccttccag ctttttaact ttccaaactt
     2461 ctagctgttc atagagataa gaaactcttc gtttgattta gttcctctta atcggtcaag
     2521 taaaaactcc attgcttcca ttgggttcat gtcggctaag tgatttctta aaatccacat
     2581 acgctccaac attttgcggt caagtaataa atcttcacgt cttgtacttg aagcaactaa
     2641 gtcaattgcc ggataaacac gtttgttgct taacttgcgg tcaagctgca actccatgtt
     2701 acctgtgcct ttaaattctt caaagataac ttcgtccatg cgcgaaccgg tgtcaattaa
     2761 agcagttgca atgatggtta atgaaccacc tttttctatt ttacgtgctg caccaaaaaa
     2821 tctttttggt ttgtgcagtg cattagagtc aacaccaccg gataatattt ttcctgatgc
     2881 aggagcagtg gtgttatagg ctcgtgccaa tcgggttata gaatccaata caatcactac
     2941 atcatgtccg cactcaacca aacgttttgc tttttccaaa acaaggttcg caattttgac
     3001 atgcttgtct gcaggctcat caaaggtcga agaaataact tcggctttta cactgcgtgc
     3061 catatctgta acctcttcag gtctttcatc aataagcaat actatcagat aaacttccgg
     3121 atggttggca gcaatggcat tggcaacttc ttttaaaagt actgtcttac cagtttttgg
     3181 ctgtgccaca attaacccac gctggccttt tccgattggc gaaaacatat ccataattct
     3241 tgtgctcatg gtgctgttct ctcctgcgag attaagtttc tcgaatggga aaagtggtgt
     3301 cagataatca aatggaatac gatcgcgaac aactgtaggt tcaagtccgt ttatcagttc
     3361 aacctttgta agcgggaaaa atttttctcc ttcacgtggt ggacgaactt tacattttac
     3421 tgtatcacca gtttttaatg caaatgcttt tatctgcgac tgtccaacat acacatcatc
     3481 cggtgaactt aaatagtcat aatccgatga gcgtaaaaaa ccatatcctt caggcataat
     3541 ttccaaaaca ccttctgctg taatggcatt gtcaaactca aaatagtcgt tctgctgacg
     3601 gaaattattc tgttgttgat tgtccggaaa tctgcgctct ctgtttctgt tctgaaaatc
     3661 gttttgttgg ttttgattat tattattatt attttgttgt tgttgttgtt gctgaggctg
     3721 ctgctgaggt ctatcttcag tatgtactct tgcaggcctt gcaggcgttt cagcttcctg
     3781 cggtgcagaa gtaacttccg gggcttttat gacattaggc tgaggcaatt catttttagc
     3841 aggtgcacct tgtggaataa ttctttttct tcttggctta tcggcatcgt cctgagtttt
     3901 tgcttctgca gatacttcag aggttgcgat tacagatgca ggttcttcta aaggcttttc
     3961 tgtaacagtt ggtgcggcag caggtttctg ttcttttttc tcatgacgcg gcttctcatg
     4021 acgtggtttt tcatctttgg gcttatcggc tttttttgat tttgccagtt caggatgaat
     4081 ggcctgatgg tcgagaattt tataaataag gtcttgcttt tttaagcctt cgtagttttc
     4141 aacattaagc tctttggcaa tgtcgcgtag ctcggctaca agcttgtcac taagttcgag
     4201 aatatcgtac atgaatttgt atgtataagg taaaaagaat tatgttcttg tggatgataa
     4261 aatattattg gaatcggatt tttgaacggg gaaaaatgct ttggctttcg ttcaaattct
     4321 gcgcaaacct ataacaatta tttttctttt gcaaaaaaga ttctttaaaa agcataattt
     4381 caccacataa cctatttttg gcacattaaa atttcagaaa catgattcaa aggattcagt
     4441 cagtctatct tctat
//
LOCUS       DAOOOL010000176         4429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2200_length_4429_cov_98.497028, whole genome shotgun sequence.
ACCESSION   DAOOOL010000176 DAOOOL010000000
VERSION     DAOOOL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2200_length_4429_cov_98.497028"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..846
                     /locus_tag="PKU82_10920"
     CDS             451..846
                     /locus_tag="PKU82_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83424.1"
                     /translation="MNIHASQNTPYYSRSPRAIGSVAAYIKLLLNVPLLFAVVFLVSR
                     MAFSFTQNFHNKKRPNRFALINEVQARLQTVSFIEMVQQQTKGKNLLATKSIKTHRQQ
                     NTAVKKYLLQSAFYNGGYIDPPKIETKFS"
     gene            964..>4429
                     /locus_tag="PKU82_10925"
     CDS             964..>4429
                     /locus_tag="PKU82_10925"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83425.1"
                     /translation="MLWTTGAQAQTTVTIGSGTGTSTYWPLYYFYDFSYTQQIIYASD
                     ITSGGWGGGSGLITKIRFKPKNNVASTANWKDWVIYMGNTTKTTFTSTTDWIMPGSMT
                     TVFNASIPSSLTANVWMELTLSTPFLWDGTSNLVVAVDENVDAYGSSPNWAGYAPGAN
                     RGIYFYRDNVDVLPASPPAGTRNGAATNVAQMQLDITSSVPCAGTPTPGTASSSVNPV
                     CQNNATNLVLTGYSSGLGITYQWQTSTTPGGPYTDISGATTTTYSYSPATAGTEYIVC
                     KVTCTNSSMDALSNEVTLGVNASPSGTATGPATLVTYQNGTFSVTGYPVGTTFQWQSS
                     TASCATGFSNIPAATADNVLLNSSAGGTFYIRCMLTNAGCSAPSNCVTLVVTVAGNNV
                     CSANTLNVGVNGPYTNVGATVETGEVAPPAAGCSVQNGWCNSTLSNTVWFTFTPPASG
                     QYSFRLDPSRTLWDTQFALWNASSCSPFGGFTMIAANDDSLNSPFNSYIAPVCLNAGQ
                     TYYFQFDGYSGTNNNWGILVTQVNTVTAGISGSQTGCSAVNLTATGGSTYLWSGGLTP
                     NTDANTFTASGPYTVTVTSAQGCTSQATANVVINVPSTDPGSATSNAPFDNICAGGNV
                     TLSVGGGTLGTGAQWQWYSGSCGGTPVGSGSSITVSPSVTTNYFVRAEGVCGNTMCVF
                     ITITVNTGIPAQAVTIPIVGLPAYACNATAATLSIPAVANATFYTWDGPTGTTFDGFP
                     SPYSSLAPTVNVVFGNPNGSGYRIGVQAGNACGSSARKSEWLRGIVSTPAAVSGVTTT
                     CASTSGTYTTGPVDGATSYLWTITGDASVIGTGTSVTVNFGPAWAGGTLCVRAQTPCY
                     TSPSKCLVIGTSASPLNTISGPLSACPNDVLTFSVPASSGAASYAWTVPANSSISGGA
                     GTNSIQVTFAPNYNNLGNICVNVTSICGVTSATKCRTVAPGVPSVPASITGATNGLCG
                     QSISYSTPFSPGTTYNWTAPGTITGNGNNAVSIAYGSFTTGQVCVTASTGCGTSAARC
                     VAIKGAPNSPIAITTSPASWCANTSGVEFNANVSNTAGSYTLSWTYPSMPVANYVSGG
                     GNTGQLILDWGTGNGNITVTATNSCGAGSKAYVATIGCREGQLATANSLNVYPNPTAG
                     VLNVEYTAE"
BASE COUNT         1348 a          921 c          937 g         1223 t
ORIGIN      
        1 taaacttata tcaggattaa ttaatcaaac tgtaaacttt ttttaggacg agtcatgctg
       61 ttcgaagcac agcttctata cataaggccc acgaagcagg ggctttgcgg gtacggtgtt
      121 gttcattgct gttcgaagca tagcttctat acataaggcc cacgaagcag gagcttcgcg
      181 ggtacggagt tcattgctgt tcgaagcaca gcttctatac ataaggccca cgaagcagga
      241 gcttcgcggg tacagccact atgccaaact tttctttaaa actttatatc acacaaaatc
      301 agccaattaa aatttttggc tataaattat tgaatgccga tgaaataaaa aagttgctta
      361 attaaaattt catactatat ttgaaaacga aaagaagtaa gaagtctttt cttagaagta
      421 aacgcaacag aataaccaaa accgaattgc atgaacattc atgcttcaca aaacactcct
      481 tattacagcc gcagcccaag ggcaattggc agtgtagcag catatataaa gttgttgctg
      541 aatgtgcctt tgttgtttgc cgtagttttt ttggtaagcc gcatggcttt ttcttttaca
      601 caaaacttcc acaacaaaaa acgacccaac cgctttgccc ttattaacga agtgcaggcg
      661 cgtttgcaaa cagtatcttt tatagaaatg gtgcagcagc aaacaaaagg caaaaacctg
      721 cttgcaacaa aaagtattaa aacgcacaga cagcaaaaca cagcagtaaa aaaatattta
      781 ctgcaaagtg cattttataa tggcggctat attgacccgc caaaaattga aacgaaattt
      841 tcataaattt ttttaatgta aataaaatat taaccaatta aattataaca gatgataact
      901 aaaaatcaaa caaaaaaacg aacgtcttta acaagactat ttctttgtct gacagcttta
      961 attatgctgt ggacaacggg cgcacaggcg caaactactg ttaccattgg tagcggaaca
     1021 ggcacaagta cttattggcc actttattac ttttatgact ttagctacac gcaacaaatt
     1081 atttatgcct ctgacattac ctcaggtggc tggggtggtg gatctggatt gattaccaaa
     1141 attcgtttca agcctaaaaa caatgttgca agcactgcaa attggaaaga ctgggtgatt
     1201 tatatgggta atacaactaa aactaccttt acttctacta ctgattggat tatgcctgga
     1261 agcatgacaa cggttttcaa tgcttcaatt ccatcaagtt taactgcaaa tgtatggatg
     1321 gaactgactt tgagtactcc ttttttatgg gatggaacaa gtaatttagt tgttgcagtt
     1381 gatgaaaatg ttgatgcata tggtagcagt cctaattggg caggttatgc gccaggagca
     1441 aataggggga tttattttta cagggataat gtagatgttc ttcctgcatc accccctgca
     1501 ggtacgagaa atggagcagc aacaaatgtt gcacaaatgc aattagatat tacttctagc
     1561 gtaccttgtg ccggtacacc tacacccggc acagcaagtt cctctgttaa ccctgtatgt
     1621 cagaataatg caaccaacct tgttctaact ggttattctt ccggacttgg aatcacatac
     1681 caatggcaaa catcaacaac accgggtggc ccttatacag atattagtgg tgctactaca
     1741 actacttatt cttattctcc tgcaactgca ggaacagaat atatagtttg taaagtaacc
     1801 tgtactaata gcagcatgga tgcattgtca aatgaagtaa ccttaggggt aaatgcttca
     1861 ccaagtggca cggcaacagg tcctgcaacg ttagttacct atcagaacgg tactttttct
     1921 gttacaggtt atccggttgg aacaacattc caatggcaat cgtctacggc aagctgtgca
     1981 acaggtttta gtaatattcc ggctgcaact gcagataatg tattgctaaa ttcaagtgca
     2041 ggtggcacat tctacatccg ttgtatgcta accaatgcag gatgcagtgc accttctaat
     2101 tgtgttaccc tggtagtaac agttgcagga aataatgtat gttctgcaaa tactttaaat
     2161 gtaggtgtta atggcccata taccaatgtg ggtgccactg tagaaacagg tgaggttgca
     2221 cctcctgcag ctggttgcag cgtgcaaaac ggatggtgta actcaacatt gagtaataca
     2281 gtatggttta cttttacacc acctgcaagt ggacagtact cattccgttt ggatccatca
     2341 cgcacgcttt gggatacaca gtttgctttg tggaatgcat cttcatgctc gccatttgga
     2401 ggctttacga tgattgcagc taatgatgat tccttaaatt caccattcaa ttcatatata
     2461 gcacctgttt gtttaaatgc aggacagacc tattactttc agtttgatgg ttatagtgga
     2521 acaaacaaca actggggcat tttagtaaca caggtaaata cagttactgc aggcatcagt
     2581 ggctcgcaaa caggctgcag cgctgttaac ttaacagcta caggcggcag cacctatttg
     2641 tggagtggtg gtttaacacc aaatacagat gccaacactt tcacggcttc aggtccctat
     2701 acagtaacag ttacttcagc tcagggatgt acatcgcagg caactgctaa cgttgtaatc
     2761 aacgttcctt caacagaccc cggatcagcc accagcaatg caccgtttga taacatttgt
     2821 gcaggaggca atgtaacctt atctgttggt ggtggtacat taggtaccgg agcccagtgg
     2881 caatggtaca gtggttcttg tggaggcaca cctgtaggtt caggcagcag tataaccgtt
     2941 tctccttctg ttactacaaa ttattttgta cgtgcagagg gtgtttgtgg caacaccatg
     3001 tgtgtcttta ttaccattac tgttaataca ggcataccag cacaggcagt aacaattcca
     3061 attgttggat tacctgctta tgcctgcaat gctacagcag ctacattaag tattcctgca
     3121 gttgccaatg caacattcta tacatgggat ggccctacag gaacaacatt cgatggtttt
     3181 ccatcacctt attcttcttt agcaccaaca gtaaatgttg tatttggtaa tccaaacggc
     3241 tcaggttata gaataggcgt acaggcaggt aatgcatgtg gaagttcagc gcgtaagagt
     3301 gagtggctgc gtggtattgt tagcactcct gcagcagtaa gcggagtaac aacaacatgt
     3361 gcaagcactt caggaaccta tacaacagga ccggtagatg gagcaacatc atatctgtgg
     3421 actattacag gcgatgcctc agtaattggc accggtacca gtgttacagt aaactttggt
     3481 cctgcatggg ctggtggtac actgtgtgta agagcacaaa caccatgcta cacatcacca
     3541 tctaagtgtc ttgttattgg aacttctgca tcaccattaa ataccatcag tggcccattg
     3601 tcagcatgtc caaatgatgt attgaccttt agtgtacctg catcatcagg cgcagcaagc
     3661 tatgcatgga cagtacctgc aaactccagt atttcaggtg gagcaggaac aaattctatt
     3721 caggtaactt ttgcaccgaa ctataacaac ttaggcaata tttgtgtgaa tgtaacaagc
     3781 atttgcggtg ttacatcagc aaccaagtgc agaacggtag cacctggagt tccatcagta
     3841 cctgccagta taacaggagc aacaaatggt ttgtgtggtc aaagtatcag ctacagcaca
     3901 ccatttagcc ccggaacaac ttataactgg acagcacccg gaacaattac cggaaatggt
     3961 aataatgcag taagcattgc ttatggttca ttcactacag gtcaggtatg cgtaacagca
     4021 agcacaggtt gtggcacaag tgctgcacgt tgtgtagcta ttaaaggagc accaaactca
     4081 ccaatagcaa ttacaacatc acctgcaagc tggtgtgcaa acacatctgg cgtagagttt
     4141 aatgccaatg taagcaacac agccggttct tataccttgt catggacata tccatcaatg
     4201 cctgtagcaa attatgtttc aggaggtgga aacactggac agttaatcct tgattgggga
     4261 acaggaaacg gaaacattac cgttactgca actaacagtt gcggagccgg ttctaaggct
     4321 tatgtagcta caataggttg tagagaagga caattggcta cggctaatag tctgaatgta
     4381 tatccaaatc caacagcagg tgtgttgaat gtagaataca cagcagaga
//
LOCUS       DAOOOL010000177         4424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2208_length_4424_cov_34.626001, whole genome shotgun sequence.
ACCESSION   DAOOOL010000177 DAOOOL010000000
VERSION     DAOOOL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2208_length_4424_cov_34.626001"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKU82_10930"
     CDS             <1..403
                     /locus_tag="PKU82_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ83426.1"
                     /translation="LTGIFGLPNVSGVGISFGIDRIYDVMLEMNLFDQLKLSETTSQV
                     LLANFGREEEQFSLKILNELRQAGINAEIFPEYSAKMKKQMSYADSKKIPFVVLIGKD
                     EMQSGSLTIKDMATGQQEKLTLSKIISKLSK"
     gene            438..1934
                     /gene="hutH"
                     /locus_tag="PKU82_10935"
     CDS             438..1934
                     /gene="hutH"
                     /locus_tag="PKU82_10935"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOZ83427.1"
                     /translation="MKHQISSKHLDFNTLKLIIDNKATLELSEDAVKRITHCRSYLDN
                     KVSSLAEPVYGINTGFGSLYNKSIDTADLGKLQENLVMSHACGCGEEVPAAIVKLMLF
                     LKVQGLSYGNSGVQLQTVQRLIDFYNHDIFPVVYTQGSLGASGDLAPLAHLCLPLIGM
                     GEVYFKGVKVKSAEVLKKFGWQPIKLQSKEGLALLNGTQFMSAYGTHAVMQSQRMMLL
                     ADIIGALSLDAYDGRIEPFHHLIHQIRPHKGQISTALRYRQLLADSEMIKRKKAHVQD
                     PYSFRCIPQVHGASKDALDHVSYVIFNEINSVTDNPTVFPDEDLVISAGNFHGQPLAL
                     VLDFLAIALSELANISERRTYQLISGQRGLPSFLVADPGLNSGMMIPQYTAASIVSQN
                     KQLCMPASVDSIVSSNGQEDHVSMGANAATKLYKVLCNTEKVLAIELLNAAQAFEFRR
                     PLKSSAPLESFMSLFRKEVSFMNTDRILYTDIQSSVNFLQSVNVNQYE"
     gene            1971..2387
                     /locus_tag="PKU82_10940"
     CDS             1971..2387
                     /locus_tag="PKU82_10940"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOZ83428.1"
                     /translation="MAIKRNATAFWNGSGKEGKGTVSTQSTVLKQSQYSYNTRFEDGV
                     GTNPEELIAAAHAGCFSMKLSFVLGAAGFTPDAIETKCEITLDNGAVTNSHLILNAKV
                     AGISKEKFEECANEAKANCPISKLLNTNITLSATLA"
     gene            complement(2417..3121)
                     /locus_tag="PKU82_10945"
     CDS             complement(2417..3121)
                     /locus_tag="PKU82_10945"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOZ83429.1"
                     /translation="MELFYSTNIDDNIISLNEVESAHCLRVLRHKIGDSVEVTDGKGN
                     RYSGIISVASKICKIEIAEKHFEKPAITQLHIAIASPKSIDRFEWFLEKATELGIETI
                     TPLLCRFSERKHINAERCGKILLSAMKQSNRVYLPVLNEMMSFEKFIAIPSTVKKLIA
                     HCEESEKLNLEKSISAEQVIVLIGPEGDFAQHEIKLANDAGYENISLGEKRLRTETAA
                     LHVCSAFALKNRSGQS"
     gene            complement(3121..3792)
                     /locus_tag="PKU82_10950"
     CDS             complement(3121..3792)
                     /locus_tag="PKU82_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83430.1"
                     /translation="MNYYRQNKEIQKPIRIPSAVRKMLDAGESEHLDFKKEITSVHKI
                     AKTISSFANRNGGKLLIGVNDDRTICGITTEEEKFMIDEAATNYCSPIVTVAFKEWQC
                     SDKIVLEVSVPESEQKPVFAKDENEKWWVYLRINDQTLLTTKVMVDVLRKKTDSRSTL
                     IEYSEKEKALMEYLVVHKRITLKQYCKLINISRWRATRIIVNLISIGVLQANHSEKEE
                     FYTLA"
     gene            complement(3770..>4424)
                     /gene="mqnB"
                     /locus_tag="PKU82_10955"
     CDS             complement(3770..>4424)
                     /gene="mqnB"
                     /locus_tag="PKU82_10955"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOZ83431.1"
                     /translation="IIAATKQEISLFQIEKSEEIHIYKSHNVEILISGPGILATAFHL
                     TRKLKEKQYDLIINAGIAGSLDENIIPGDVVQVKSEQIADSGVEDHDNFLDLFNLGLI
                     DNNEFPFKEKWLSPKTITTPSIEKIKKVNAITVNKAHGNENSIRQLKKRTTGQIESLE
                     GAACFYVCMMLKTNVIQLRAISNMVEPRNRSNWKIELAITNLNATLNLLLDELLSSE"
BASE COUNT         1368 a          807 c          793 g         1456 t
ORIGIN      
        1 ccttacagga atttttggac tgcctaatgt cagtggtgta ggaatatctt ttggaattga
       61 cagaatttat gatgtaatgc ttgaaatgaa tttgtttgat caacttaaat tgagtgaaac
      121 aacttctcag gtattacttg caaacttcgg tcgggaagaa gagcagttta gtttgaagat
      181 tttgaatgag ctaaggcagg caggtatcaa tgcagagatt tttccggaat attcagccaa
      241 gatgaaaaaa caaatgagct atgccgactc aaaaaaaatt ccttttgtag tattaatcgg
      301 taaagacgaa atgcaaagtg gaagcctcac tataaaagat atggcaacag ggcaacagga
      361 aaagttaact ttaagcaaaa taatttctaa gctaagcaag taatagctat taatatacta
      421 agtaacaact gtcaaaaatg aaacatcaga tatcatcaaa acatctggat tttaatacac
      481 tcaaactaat cattgataac aaagctactc ttgaactttc tgaggatgca gttaaacgga
      541 ttacgcattg ccgcagttat ctcgataaca aagtcagctc acttgctgaa ccggtttatg
      601 gtattaacac aggctttggt tctttataca ataagagtat tgatacagct gatttaggta
      661 aacttcagga aaatttggta atgtcgcatg cctgtggttg tggcgaggaa gtgcctgctg
      721 ctattgttaa gttaatgctg tttctgaaag tacagggact tagttatggc aactccgggg
      781 tgcagttgca aacggttcaa cgtttgattg atttttataa tcatgatatt tttcctgttg
      841 tttatacaca ggggtcatta ggcgcatcag gtgatttggc accgttggca catctttgtt
      901 tgccgctcat aggtatgggc gaagtgtatt ttaaaggagt aaaagtaaag tcggcagaag
      961 ttctcaagaa gtttggatgg cagccaatca aactgcagtc aaaggaagga cttgcattgc
     1021 tgaatggtac acagtttatg agtgcctacg gcacacatgc agttatgcag tcgcaacgta
     1081 tgatgttgct tgcagatata atcggagcgc tttcattaga tgcttacgat ggcagaattg
     1141 aaccatttca tcatctcatt catcagattc gacctcataa gggacagata tcaacagcat
     1201 tgcgttatcg tcagttgttg gcagacagtg aaatgattaa aagaaaaaag gcgcatgtgc
     1261 aggatccata ttcattcaga tgtattccgc aagttcatgg cgcaagtaaa gatgcattgg
     1321 atcatgtaag ctacgtaatc tttaatgaaa taaattctgt tacagacaat cctacagttt
     1381 ttcctgacga agatttagtt atttcagcag gaaactttca cggacaacca ctagcattag
     1441 ttctcgattt tttagctatt gctttaagtg agttggcaaa tatttcagaa cgcagaacct
     1501 atcaacttat ttccggacaa cgtggtttgc catcttttct tgttgcagac cctggactga
     1561 attccggaat gatgattccg caatacacag cagcatcaat tgtaagtcag aacaaacaat
     1621 tgtgtatgcc tgcatcggtt gacagtattg tgtcgagcaa tggtcaggaa gaccatgtga
     1681 gcatgggcgc caatgcagca acaaaactgt ataaggtact ttgcaataca gagaaagtat
     1741 tagccattga gttactcaat gcagcacagg catttgaatt cagaagacca ttaaaatctt
     1801 ctgctccatt ggaatcattt atgagtcttt tcagaaaaga agtttcgttt atgaatacag
     1861 acagaatact ttatacagat attcaatctt cagtaaactt tttgcagtct gtaaatgtta
     1921 atcagtacga ataagtatca aattatataa ctcaaacatt aaaaatcatt atggcaatta
     1981 aaagaaatgc aacagccttt tggaatggct caggcaaaga aggtaaagga acagtatcaa
     2041 cacaaagcac tgttttaaag caatcgcaat actcctataa cacacgtttt gaagatggtg
     2101 taggcaccaa ccccgaagaa ctgatagcag cagcacatgc aggctgtttt agtatgaaac
     2161 taagttttgt tttaggtgct gcaggattta ctcccgatgc aattgaaaca aaatgtgaaa
     2221 ttacacttga taatggtgca gtaaccaact cgcaccttat tctgaatgca aaagttgcgg
     2281 gcatctctaa agaaaaattt gaagaatgtg ccaatgaagc taaagcaaat tgtcctattt
     2341 caaaattatt gaataccaac attactttaa gcgcaacatt agcttaaaaa ttatttggaa
     2401 gcaggacaat cgttttttac gattgtcctg atctgttttt gagtgcaaat gcactgcata
     2461 catgtaaggc tgcagtttct gtgcgcagtc ttttttcacc aagagaaata ttttcatatc
     2521 cagcatcatt ggcaagttta atttcatgtt gtgcaaaatc gccttcgggg ccaataagta
     2581 caattacctg ttctgctgaa attgattttt caagattaag tttttcgctc tcctcacaat
     2641 gtgcaatcag tttttttaca gttgaaggaa ttgcaataaa tttttcaaac gacatcattt
     2701 catttaatac tggcaaataa actctgttac tttgtttcat tgcagataat agtattttac
     2761 cacatcgttc agcatttata tgtttacgtt cgctgaagcg gcataacagt ggtgtaattg
     2821 tttcaatacc caattcagtt gctttttcta aaaaccattc aaacctgtca attgactttg
     2881 gagatgcaat agcaatgtgc agttgtgtta ttgcaggttt ttcaaaatgt ttttctgcta
     2941 tttcaatctt acagattttt gaagccaccg aaataattcc tgaatatcgg ttaccttttc
     3001 catcagtaac ttcaacagaa tcgccaattt tatgtcgtag aactcgcaag caatgtgcgc
     3061 tttctacttc gtttaatgaa attatattgt catcaatgtt tgtgctgtaa aataattcca
     3121 tcaggcaagt gtataaaatt cttctttctc ggagtgattg gcctgtaaaa caccaatact
     3181 aatcaggttt acaataattc ttgttgcccg ccaacgcgag atattgatga gcttacagta
     3241 ttgtttcaat gttattcttt tatggacaac taaatactcc atcaaagctt tttctttttc
     3301 agaatattca atcaatgttg acctgctatc ggtctttttt cgtaatacat caaccatcac
     3361 cttagttgtc agcaacgtct gatcattaat tcgtaaataa acccaccatt tttcgttttc
     3421 atcttttgcg aagacaggtt tttgttcact ctccggtaca gaaacttcca gaacaatttt
     3481 gtccgagcat tgccactctt taaatgcaac tgtaacaata gggctacaat aatttgtagc
     3541 agcctcatca atcataaatt tttcttcttc tgttgtaatg ccacaaatag ttctatcatc
     3601 atttacacct atcaataatt ttcctccatt acgatttgca aaagagctta ttgtttttgc
     3661 aatcttatgc acactggtga tttctttttt aaaatctaag tgctcactct caccggcatc
     3721 aagcatcttc cttactgccg atggaatcct tattggcttc tgtatttctt tattctgacg
     3781 atagtaattc atcgagtaaa agatttaaag tagcatttaa atttgtaatt gctaactcaa
     3841 ttttccagtt gctgcgattg cgaggctcaa ccatgttact gatagctcta agctgaataa
     3901 catttgtctt taacatcata caaacataaa aacatgcagc accttccagc gattcaatct
     3961 gccctgtagt tctttttttc agttgcctaa tactgttttc gttaccatgt gctttgttga
     4021 cagtaatagc attaactttt ttaatttttt ctattgaagg tgttgtaatt gttttcggag
     4081 aaagccattt ttctttaaaa ggaaattcat tattgtcaat caatcctaaa ttaaaaaggt
     4141 caagaaaatt atcatgatct tcaactccgc tatctgcaat ctgctcagat ttaacctgaa
     4201 caacatctcc gggaataata ttttcatcta aacttccggc aataccggca ttaataatca
     4261 ggtcatattg cttttctttc agttttcttg tcagatgaaa ggcagttgcc agaattcctg
     4321 gccctgatat caatatttct acattatgac tcttataaat atgaatttct tcagattttt
     4381 ctatctgaaa tagacttatt tcttgttttg ttgctgcaat tatg
//
LOCUS       DAOOOL010000178         4411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2219_length_4411_cov_35.993572, whole genome shotgun sequence.
ACCESSION   DAOOOL010000178 DAOOOL010000000
VERSION     DAOOOL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2219_length_4411_cov_35.993572"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1373)
                     /locus_tag="PKU82_10960"
     CDS             complement(234..1373)
                     /locus_tag="PKU82_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOZ83432.1"
                     /translation="MEFAATENQQMIAQTVKDFAEKHIRPNVMTWDEAQDFPVDVFKK
                     MGDIGLMGLLVPEQYQGAGLGYFEYVTAIVEVARVCGSVGLSMAAHNSLCTGHILAFG
                     NEEQKKKWLPKLATGQWIGAWGLTEANTGSDAMRMQCVAKQDGDSWIINGAKNWITHG
                     KSGDVAVVLARTGELLDSNGITAFVVERGTPGFKAGKKENKLGMRCSETAEMIFEDCR
                     IPASNILGKVGDGFKQAMKILDGGRISIAALSLGIAKGAFDASVKYAKERQQFGQPIA
                     NFQAIGFKLADMATKIEASELLILQAADLKNRGEKVTRQSAYAKYYASEVAVYVSTEA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVIARELLK"
     gene            1537..2823
                     /locus_tag="PKU82_10965"
     CDS             1537..2823
                     /locus_tag="PKU82_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ83433.1"
                     /translation="MMHATIEKGAYTASDMAETLIGSEIIKLAGEVNEQIKKGNKIFN
                     LTIGDYDPAVFPIPDALKNEIIKAYQNGYTNYPVANGQPELRNAVAGYINKLQKLQYS
                     SNEILISGGARPLIYALYKTVVNPGERVVFPIPSWNNNHYCHLSQAEAVAVETQPQNN
                     FMPLASELKSHLSSAALVALCSPLNPTGTVFSSSQLMEICELILQENKRREGKKPLYL
                     MYDQIYMALTFGKTKHVDPVSLLPEMRPFTIYIDGLSKAFSATGLRVGWAFGPVGIID
                     KMKSILSHVGAWSPKAEQVATAHFLTQEKEVDVFLSHYKSEVEKRLNAFYDGFIKMKN
                     DGLPVNAIDPQAAIYLTVQFDILNKVMPDGKRINNSNDITSFLINYAKVALVPFSAFG
                     ATTTSNWFRLSIGTARMEDIEQMFVSLRKALALLGD"
     gene            complement(2803..3672)
                     /locus_tag="PKU82_10970"
     CDS             complement(2803..3672)
                     /locus_tag="PKU82_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ83434.1"
                     /translation="MHISILLWGFTGIFGKAIEMNAIMIVWYRMIISAVALIPFMVKH
                     KQFILPDKKRLASIIITGFVVCLHWLTFYASIKASNVSIALSCFASVSLFSALLEPLF
                     YRKKINKQNIILALFVLVGIYIIFAFQQLYAMGIVLALISALLGALFTILNKIFVSND
                     EPAPVTFIELISGFVFLSVLLPLILPAFNVSFQIPSKIDWVWLLLLSVVCTSIAFTLS
                     LEALKKVSAFTMNLSVNLEPLYSIVLAIILFKENELLNNGFYIGAVIVISSVFIHSLM
                     QYRVRDKSVTEQG"
     gene            complement(3697..4269)
                     /locus_tag="PKU82_10975"
     CDS             complement(3697..4269)
                     /locus_tag="PKU82_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOZ83435.1"
                     /translation="MNHLKQKIILASRSPRRQQLLQMMGIEFEVRTKETVEDYPEHLQ
                     NEQIVLYLASKKAIAFENEIQENELLISADTIVVHQNKVLNKPADFNEAIEMLTTLKG
                     NMHEVYTGVCIASIHKKELLYDRSEVYFRNYTTEDMHYYISNFKPYDKAGSYGIQDWF
                     GLTCIEKINGCFYNVMGLPTRKVYQQLINF"
BASE COUNT         1357 a          874 c          799 g         1381 t
ORIGIN      
        1 gtaaaaaata catgggtaga gtctgtgcct gatgctaatg gtgtaagcac tattactgca
       61 ggacaaacta ctacaacaaa gagtaatcct tcaatgaaac aaggaaagga ttcaggcttt
      121 ggtccgaatg ttactgctaa aataaaatta ggttggttgt tctgatagga aaatcaccta
      181 aacaaaaaag gctgctcaat tgagcagcct tttttgtttg tgctttaatc ctattacttc
      241 agcagttctc tggcaattac taatttttga atttcggaag tgccttctcc aatggtgcaa
      301 agtttactgt cgcgataaaa tttctctacc ggaaaatctt tggtataacc atagccgcca
      361 aaaatctgca cagcttctgt gcttacataa acagccactt ccgaagcata atattttgca
      421 taagccgact gacgggtaac tttttcacca cggtttttta aatcagccgc ttgtaatatt
      481 aaaagttctg atgcttcaat ctttgttgcc atgtcggcca acttaaaacc tattgcctga
      541 aagttggcaa tgggctgtcc gaactgttgt ctttcttttg catatttaac agatgcatca
      601 aatgcaccct ttgcaatacc taatgatagt gcagcaatag aaatgcgtcc gccatccaat
      661 attttcattg cctgtttaaa tccatcgcca accttaccta aaatatttga agccggaatt
      721 ctgcaatctt caaaaatcat ttcagcagtt tcgctgcatc gcattcctag tttattttct
      781 tttttgcctg ctttaaatcc cggtgttccg cgctccacca caaatgctgt aatgccattg
      841 gagtccagca attcaccggt gcgtgcaagc actactgcca catcaccgct tttgccatga
      901 gtaatccagt tttttgcacc attaattatc caactgtcgc catcttgctt tgccacacat
      961 tgcatgcgca tggcatcgct gccagtattg gcttctgtta gtccccatgc acctatccat
     1021 tgtccggtag ccaatttagg taaccatttc tttttctgct cttcgtttcc aaaagctaaa
     1081 atgtgccctg tacataatga gttatgcgca gccattgaca agcctaccga tccgcaaact
     1141 cttgctacct ctacaatggc agtaacgtat tcaaaataac ctaaacctgc accctgatat
     1201 tgctccggta ctaatagtcc cataagcccg atatcgccca tctttttaaa aacatcaaca
     1261 ggaaaatcct gcgcctcatc ccacgtcatc acattgggtc tgatatgttt ttcggcaaaa
     1321 tcttttaccg tttgtgcaat catctgctga ttttctgtag ctgcaaattc cattctctaa
     1381 ggtattttta ttaattgaat tttttttttc agaagtgtgc aaagataaaa aaacaaaccc
     1441 atttttaggg tgatatttta ttattccggt aaatgagtaa acttgcattg ttaactcata
     1501 ttttctgaat actttcggag caaatttttg aatgaaatga tgcatgcaac aatagaaaaa
     1561 ggagcatata ctgcctctga tatggcagaa actttaattg gttcggaaat cataaaactc
     1621 gctggcgaag taaacgaaca aattaagaaa ggaaataaga tttttaatct gactattggc
     1681 gattatgacc ctgccgtttt tcctattccc gatgcgctaa agaatgaaat tattaaagcc
     1741 tatcagaatg gatataccaa ttatcctgtt gccaacggcc agccagagtt gcgtaatgct
     1801 gttgcaggtt atatcaataa attgcaaaaa ttacaataca gtagcaatga gattttaatt
     1861 tccggtgggg cacgaccact tatttatgca ttgtacaaaa cagttgtaaa ccccggtgag
     1921 agagttgttt tccctattcc atcatggaac aataatcact attgtcattt gtcgcaggca
     1981 gaagctgttg ctgtagaaac acagccacag aataatttta tgccgcttgc atcagaactc
     2041 aaatcacatc tgtcatcggc agcattggtt gcactttgtt ctcctctaaa tcctacaggt
     2101 acggtcttta gcagtagtca gttaatggaa atatgtgagt tgatattgca ggaaaacaaa
     2161 agaagagaag gtaagaagcc attgtatctt atgtacgatc agatttatat ggctttgact
     2221 tttggtaaaa caaaacatgt tgacccggta tcactgcttc ctgaaatgcg acctttcaca
     2281 atatatattg atggtctttc caaagctttt tctgcaacag gtttgcgtgt aggctgggca
     2341 tttggtccgg taggaattat tgataaaatg aaatctattc tctcgcatgt aggcgcatgg
     2401 tcgcctaagg cagagcaggt ggcaacagca cattttctga cgcaagaaaa agaggtagat
     2461 gtatttttaa gtcattacaa atctgaagta gaaaaaagac tgaatgcatt ttatgatggc
     2521 tttataaaaa tgaaaaatga tggattgcct gtcaatgcca ttgacccgca agcagccatt
     2581 tacctcaccg ttcagtttga tattttaaat aaggtgatgc ctgatggtaa acgtataaat
     2641 aattcaaacg acattacatc gtttctcatt aactatgcca aggtagcatt agtacccttt
     2701 tctgcatttg gtgcaacaac aacttcaaac tggtttcgac tttctatcgg gactgcccgt
     2761 atggaagata tagaacagat gtttgtttct ttacgcaaag ctttagcctt gctcggtgac
     2821 tgacttatct ctgacccggt attgcatcag actgtgaata aaaacagaac tgatgacaat
     2881 gactgcgcca atgtagaaac cgttattgag caattcattt tctttgaata aaattattgc
     2941 aagaacaatg ctgtaaagcg gttctaaatt tactgagagg ttcatggtaa aagcactcac
     3001 tttttttaat gcctccaatg aaagtgtaaa ggcaatagag gtgcaaacta cactcagcaa
     3061 caacagccac acccaatcta tctttgatgg aatttgaaaa ctaacattaa aagccggaag
     3121 gatgagtgga agaagaacag ataaaaacac aaatccactg attaattcta tgaatgttac
     3181 cggtgcaggc tcgtcattgc tgacaaatat tttattcaga atagtaaaaa gggcaccaag
     3241 caatgcactg attaatgcta gaacaatgcc catggcatat aactgctgaa aagcaaaaat
     3301 gatatagatg ccaacaagta caaacaacgc taagatgatg ttttgtttat ttattttttt
     3361 gcgataaaac aatggctcca gcaatgcaga aaatagtgaa accgaagcaa aacaactaag
     3421 tgcaattgac acattagaag cttttatgga ggcatagaag gtgagccaat gcagacagac
     3481 tacaaaacct gtgatgatga tggatgctaa tcttttttta tcaggcagta taaactgttt
     3541 gtgttttacc ataaatggta tcaatgccac tgcactgatg atcattctgt accatacaat
     3601 catgatagca ttcatctcaa ttgccttacc gaatattccg gtaaatcccc acaacaaaat
     3661 ggagatgtgc atttgcagat aagcctttcg catgaactaa aaatttatta attgctgata
     3721 aacttttctt gttggcaagc ccataacatt ataaaaacat ccgttaattt tttctataca
     3781 tgtcaatcca aaccaatcct gaattccgta agatcctgct ttgtcatagg gcttaaaatt
     3841 actaatgtaa taatgcatgt cttctgtagt gtagttgcga aaataaactt cactcctgtc
     3901 atagagcaat tcttttttgt gaatggaagc aatgcaaacg cctgtataaa cttcatgcat
     3961 gttgccttta agagtagtga gcatttcaat agcttcgtta aagtctgccg gtttattcag
     4021 cactttgttc tgatgtacaa caatggtatc tgcgctgatg agtaattcat tttcctgaat
     4081 ctcattttcg aaagcaatag ctttttttga agcaagatat aagactatct gttcgttctg
     4141 caagtgttcc ggataatctt caacagtttc ttttgttctt acttcaaatt caatacccat
     4201 catttgcaaa agctgctgtc tgcgtggtga acgtgaagcc agaattattt tttgtttgag
     4261 atgattcatt gcatcaggat tgatgtgcaa aggaaatggt tttatgcttg catcggaatt
     4321 tatactctta tgtgtattta cttaatcaac aattttttta aatctttttt gcttccgtta
     4381 aacacattgg catcataaga agaacttgaa c
//
LOCUS       DAOOOL010000179         4348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2266_length_4348_cov_145.254601, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000179 DAOOOL010000000
VERSION     DAOOOL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_4348_cov_145.254601"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKU82_10980"
     CDS             <1..334
                     /locus_tag="PKU82_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83436.1"
                     /translation="KQIDYDGKFEYSFVKTAMIGDLKNVILSAAPNPYRGSTKINYSI
                     PTDGSVVILITDMTGRILKNIDAGMQTKGSYSIPFSAIADGHPSGVYNVTLFFNDKPY
                     RLKISEYR"
     gene            complement(340..3327)
                     /locus_tag="PKU82_10985"
     CDS             complement(340..3327)
                     /locus_tag="PKU82_10985"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ83437.1"
                     /translation="MRRIVLLVFGFVCIVKIAIGEKPYFQQRVDYKISVSLDDKKNFL
                     SGFEELIYRNNSQDTLRELFFHLWPNAYKDRSTALCKQLVSQGNMSLYFANDEQRGFI
                     DSLNFRVDDKVVSLVYDSINIDICKIVLNKPLLPNDTILITTPFRIKIPDAKFSRLGH
                     VGQSYMISQWYPKPAVYDKDGWHTMPYLSQGEFYSEFGSFLVDITVPANYVVAASGYL
                     KTPSEVEFINKKIKTESNQINFDKQNTFPESESSSKSIRFALDSVHDFAWFADKRFRI
                     VSKVIKLASGHEVECRAYYTPANSYVWHRAADYVAEAVLFYSKYVGEYPYGVCTAVDG
                     TIAAGGGMEYPTITVIGSTGNSMVLNQSIAHEVGHNWFYGILASNERKLPWMDEGINS
                     FYEQLYMIQYYARESAAAYQLTGVPLKIAKTFGADKMSFAGFNKISYLLTARTNDDQS
                     SGLTSEYFSNLNYGADIYSKIPLCFSQLRDFLGHEKYDSCMQTYFNTWKFRHPDEAAI
                     QNVFETTSGKSLGWFFNGMIDSNSKSDAKICKVNTDSGGTDVLLKNRGNLAMPVNVSF
                     YNKEKLIASQWTGVFDGKFKLSSSVSGADKIVLDTYDQSLDLNPFNNSMKTKGVFRKW
                     KPLQLRFLTMLENPERTQLFYLPAVAYNNYNGWMAGVILHNYSLLSKKFEFGIVPMYA
                     FKSKSVAGVATASYQFNIYNGWFDRIIAKISPTLFDEGLSENRSIFKGYFSVPASLSF
                     RIRKSQYKPYSEKYVNIIHHYINQTFSTQNNSSATNEINITQLEFASFNYSKTDPGSL
                     SFILEKGGHYAKLTGTWKKEITLTRRKKDLSLRFFAGTFLDNSDYGGEANFRSSAWRG
                     TDDYSYSGLWFGRNEYDGFWAHQMQRADGAMAAYYPVRTDHWLAALNVSVKTPVSGLR
                     LFADGITFYNANKVSPGATVVRYDAGIMLSIVHDAFEIYWPLIVSKEVRDYYDLNDIK
                     LADRIRFVFNINKLNPVLLRKMR"
     gene            complement(3331..4287)
                     /locus_tag="PKU82_10990"
     CDS             complement(3331..4287)
                     /locus_tag="PKU82_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ83438.1"
                     /translation="MGLFFALITTLSWSIGIFPFTEASRKLGVNSLNHFRLFLAVALL
                     FATALLINHSEFINLFNEQYVQAWIWFGLSGVVGLALGDYFYFGLYAIMGARIGSIFS
                     TLSPAAALVTSYFFVDERINFIGVIGIFLTMAGVIFISISKSENLSAEEGRYGSDVKG
                     VLFGVLAAFCQGVGLVLAKKGFVVLEQEGKFIHPINATFVRMVVSLATLILITTIFRK
                     WKEVLRPLKSNSAGLKYAAVGTFFGPVFGVCMSLYTIAVLEATVAQTIFSLVPVVAAL
                     IARYFYKEKFTLASVIATIIAVAGVVLLIRRNEVLECLYKSN"
BASE COUNT         1486 a          875 c          633 g         1354 t
ORIGIN      
        1 gaagcagatt gattatgacg gtaagtttga atatagtttt gtaaaaactg ctatgattgg
       61 tgacttgaaa aatgttatac tgagtgctgc acctaatcca tatcgtggta gcacaaaaat
      121 taattactca attccaactg atggcagtgt tgtaattcta attactgata tgacaggtag
      181 aatattgaaa aatattgatg caggaatgca aactaaaggc agctacagca ttccgttcag
      241 tgctattgct gatggacatc cttcgggagt ttataatgta acactattct tcaatgataa
      301 accgtataga ttgaaaattt cggaatacag ataaaaatct cagcgcattt tgcgtagcaa
      361 gacaggattc agtttgttaa tattaaaaac aaaccggatc ctgtctgcta gttttatatc
      421 attcaagtca tagtaatctc ttacttcctt tgaaacaatc agtggccaat aaatctcaaa
      481 tgcatcatga actatactta acatgatacc ggcatcgtaa cgaactaccg ttgcaccagg
      541 acttactttg tttgcattat aaaatgtaat tccatcagca aataatctca agcctgatac
      601 cggtgtttta actgaaacat ttaatgcagc aagccaatga tctgtacgga caggataata
      661 tgctgccatt gcaccatctg ctctttgcat ttgatgcgcc cagaatccat cgtattcatt
      721 cctgccaaac cacaaacctg aataagaata atcatcagta ccgcgccatg cacttgaacg
      781 gaaattagct tctcctccat aatcagaatt atctaaaaat gttcctgcaa aaaaacgcaa
      841 tgacaaatct ttttttctcc gcgttaatgt tatttccttt ttccatgttc ctgttaactt
      901 agcatagtga cctccttttt cgagaataaa tgacaatgaa ccggggtctg ttttactgta
      961 attaaaactt gcaaactcaa gttgtgtaat attgatttca ttggttgctg acgaattatt
     1021 ttgtgtactg aatgtttggt ttatataatg atgaataatg tttacgtact tttctgaata
     1081 tggcttatac tgtgacttac ggattctaaa actcaatgat gcaggaacag aaaagtatcc
     1141 tttaaagata cttctatttt cagataaccc ctcatcaaat agtgtaggtg atatcttagc
     1201 aattattctg tcaaaccaac cattgtaaat attaaactga taacttgcag ttgcaacacc
     1261 tgcaacagat tttgatttga atgcatacat tggtacaatt ccaaattcaa attttttact
     1321 caacaatgaa tagttatgca gaataacgcc tgccatccat ccgttgtaat tattatatgc
     1381 aacagcaggc agataaaaca actgtgtcct ttcaggattt tcgagcattg ttaaaaaacg
     1441 aagttgtaat ggtttccatt ttctaaacac accttttgtt ttcattgaat tattaaatgg
     1501 atttaaatcc aaactttgat cataagtatc taacacaatt ttatctgcac ctgaaacaga
     1561 acttgacaac ttgaatttgc cgtcaaaaac tcctgtccat tgcgaggcaa ttaacttttc
     1621 tttattgtaa aaagacacat taacaggcat agcaagattt cctctattct ttagcaatac
     1681 atcagtccct cctgaatcag tattcacttt acagatttta gcatctgatt tcgaattact
     1741 atcaatcatt ccgttaaaaa accaacctaa cgattttccg gaagtagtct caaacacatt
     1801 ttgaattgct gcttcatcag ggtgtctgaa cttccaagta ttaaaataag tctgcataca
     1861 ggaatcatat ttctcatgcc ctaaaaaatc tctgagttgt gaaaaacata aaggtatttt
     1921 agaataaata tcagcaccat agtttagatt cgaaaaatac tcacttgtaa gtccactact
     1981 ttggtcatca ttggttctgg ctgtgagtaa atagctaatc ttattaaacc ccgcaaacga
     2041 cattttatct gcaccaaaag tctttgcaat ttttaatggc actccagtca actgatatgc
     2101 tgcagcactt tcccttgcat aatactgtat catatacaat tgctcataaa acgaatttat
     2161 accttcatcc atccaaggca actttctttc attgcttgca agtatgccat aaaaccaatt
     2221 atgccccact tcatgggcaa tactttgatt caagaccata ctattaccag tactcccaat
     2281 taccgttatt gttggatatt ccattcctcc gcctgcggca attgttccat caacagcagt
     2341 acaaacacca taaggatatt ctccaacata tttagaataa aacaaaactg cttcagcaac
     2401 ataatcagca gcacgatgcc agacataaga attagcaggt gtataatatg cacgacattc
     2461 cacctcatgt ccggaagcta actttataac ttttgataca atacggaatc gcttatctgc
     2521 aaaccaggca aaatcatgca cgctgtcaag tgcaaaacga attgacttac ttgaactttc
     2581 tgattcagga aaagtatttt gtttatcaaa attaatttga ttgctttctg tctttatttt
     2641 tttattaata aactcaactt ctgaaggagt ttttaaatag cctgaagctg ctacaacata
     2701 atttgcaggc acagttatat ccaccagaaa agaaccgaac tcagaataaa attctccctg
     2761 agataaataa ggcatagtat gccatccatc tttatcataa actgcgggct taggatacca
     2821 ttgcgaaatc atgtaagact gtccaacatg accaagtctt gaaaactttg catcaggaat
     2881 ttttattctg aatggtgttg taattaagat tgtgtcgtta ggcaataatg gtttattcag
     2941 aacaatttta catatatcaa tattaatgga atcataaact aatgacacaa ctttatcatc
     3001 aactctaaaa ttcagactat caataaatcc tctttgctca tcatttgcaa aatacagact
     3061 catattcccc tgacttacca attgcttaca caatgcagta ctcctgtctt tataagcatt
     3121 cggccaaagg tgaaaaaaca gctcacgtaa agtatcttgc gaattattac gataaatcaa
     3181 ttcttcgaat ccggaaagaa aatttttctt atcatccaat gaaactgaga ttttataatc
     3241 tacacgttgc tggaaataag gtttttcacc aattgcaatc ttaacaatac aaacaaaacc
     3301 aaaaacaagt aaaactattc ttctcatcac tcaattagat ttatacaaac attcaaggac
     3361 ttcatttcgc cttatcaata acaccactcc ggcaacagca attattgttg caattacaga
     3421 tgctaaagta aatttttctt tatagaaata acgggctatt aatgctgcca caacaggtac
     3481 taatgaaaaa attgtttgtg caactgtggc ttcaagtaca gcaattgtat aaagcgacat
     3541 acagacacca aaaacaggtc cgaaaaaagt tcctactgct gcatatttta gccctgcact
     3601 atttgatttt agtggtcgca aaacctcctt ccattttcta aatattgtag ttatcaaaat
     3661 taaagttgcc aacgaaacaa ccattctgac aaatgttgca ttaattggat gaataaattt
     3721 cccttcctgt tctaatacaa caaaaccttt cttggcaagt accaatccaa ctccctgaca
     3781 aaaagctgca agaacaccaa acaaaacacc tttaacgtca cttccatacc tgccttcttc
     3841 tgctgacagg ttttcacttt ttgaaataga aataaatatt actcctgcca tggtgagaaa
     3901 tattcctatt acaccaataa aattaatacg ttcgtcaaca aaaaaataag atgttactaa
     3961 tgctgctgcc ggtgaaagtg tagaaaaaat acttcctatc ctggctccca ttattgcata
     4021 gagtccaaaa taaaaatagt cacctaatgc taacccaaca acacctgaaa gtccaaacca
     4081 aatccaagcc tgaacatact gctcattgaa aagattgata aactctgaat gatttattaa
     4141 taatgctgtt gcgaaaagta aagctacagc aagaaataat ctgaaatgat taagtgaatt
     4201 tactccaagt tttcggctgg cttctgtaaa tggaaaaata ccgatagacc aggaaagtgt
     4261 tgtaataagt gcaaagaaca atcccatata actatctgct tccaaacaat gctgaaccta
     4321 ttcgaatcat attacttcct tcatctaa
//
LOCUS       DAOOOL010000180         4205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2376_length_4205_cov_86.666747, whole genome shotgun sequence.
ACCESSION   DAOOOL010000180 DAOOOL010000000
VERSION     DAOOOL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_4205_cov_86.666747"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKU82_10995"
     CDS             complement(<1..439)
                     /locus_tag="PKU82_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83439.1"
                     /translation="MSFGKNRVQYTDFYWTFFRFKNYDVYNYVGGKELATYVAEQAQN
                     EMDVMERTLDYRSSSRFQIVIFNKLSDLKQTNIGLELDDQAYNIGGMTKVIGNKVLIY
                     FDGNHQHLQEQIRAGMARALLEQLFYGGKSLEKEKVYSICACER"
     gene            complement(646..2892)
                     /locus_tag="PKU82_11000"
     CDS             complement(646..2892)
                     /locus_tag="PKU82_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOZ83440.1"
                     /translation="MRKLLTLSALFVILSGTQSLASSFWTAKSETQEMLSGKRQIVPD
                     KYKVYTLDVQGLKNQLLNAPMEFTMAAKQSPVVITLPMPDGSWNSFSMVESPVMQPKL
                     AAKYPFIKTYSGSGINNQPSWVRIDFTQWGFHALIRTAENDIYIDPYSMQTTSDYLVY
                     FRKDFTKSQSSMVCEFDDLQQGQSANKTLTNSMPSVNRSIGTNLRTYRLALACTGEYA
                     AYYGGTKAGALSGMVTSVNRVTGVYESEVDVRLNLIANTDTLIFLNANTDPYTNNSGG
                     TMLGENQSVVNFFIGTPNYDIGHVFSTGGGGIAYLGCICKTADKAKGVTGSAAPINDP
                     FDIDYVAHEMGHQFGGNHTFNSVTGSCQGNREAGAAYEPGSGVTIMGYAGICGSDDLA
                     AHSIAYFHTKSFDEIVDYSTLSTGNSCPVNTITFNNAPTVSGGGIFNVPVGTAFKLNG
                     YGSDPDGDSITFSWEQFDLGPAGAWSLPTGNAPMYRSYAPSTSALRLFPKLSVLLNQV
                     NAKGELITSYARSLHFRLTARDNKPNGGGVTYNDTVTTVNVVQTSAPFAITYPNVTGI
                     SWPALSTQTITWEVGGTDAAPFNEQFVNIYLSINNGTTFPITIATNVANNGSYTFTVP
                     NNPSNICRMWVEGASTGSIFFDINNKVFTITAPVGINEVNSLSQLTTYPNPADNELLV
                     YLGNNYHGQLNISLYSVNGQLVLSQTIAAKNATAQVALNVEQLPAGLYFLKAETENGT
                     AERKVMIK"
     gene            complement(2974..3726)
                     /locus_tag="PKU82_11005"
     CDS             complement(2974..3726)
                     /locus_tag="PKU82_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83441.1"
                     /translation="MLKRFVCITISLLAFIIVNAQDNKTEKKDSIEHRILLIPYDPRF
                     YLSDADKDIAEETRKDPKTIRNYFHNRSEWFTSRELKRHYPTISLLQNDTVPAYIQSA
                     GDIFSQTAFDYATPVTRSPESMMNATSDKTLTDSRTATNYLNTDVHNRYMKADIQNKK
                     VLADLSERFNTDIFVFLTQFEIKTNYKNCLDIANKIYEREIRLHFTIYDKTGKLINGN
                     YAIAVIPSNVNQMDEIILKCFPLLAKGIAASL"
BASE COUNT         1304 a          800 c          764 g         1337 t
ORIGIN      
        1 cccgttcgca tgcacaaata gaatatacct tttccttttc taaagattta cctccataaa
       61 acaattgttc aagtaaggct cttgccatgc cggcacgaat ctgttcttgt aaatgctgat
      121 gattgccatc aaaataaata agcactttgt tgcctattac ttttgtcatt cctccaatat
      181 tgtaagcctg atcatctaat tcaaggccaa tattggtttg tttgaggtca ctgagtttat
      241 taaatattac aatctgaaat cgtgacgaac tccgataatc caaagtgcgt tccataacat
      301 ccatttcatt ctgtgcttgt tcagcaacat aggtggctaa ttcttttccg cctacgtaat
      361 tataaacatc gtaattttta aatctgaaaa atgtccagta aaaatctgtg tattgtactc
      421 tgtttttgcc aaacgacatc tgcgagccct gataaaactg tgcatcagca gaatgtagaa
      481 ttataaaagt aaataagagc gtgaatacgg ctttgaaaaa tggcttcacc ttttttgttt
      541 tatgtgatat gcgaaagtag ttaatttgaa ctttatttaa accgaaaatt aaaacataaa
      601 aaaagggctg tttattcaac aaccctttca tgaattatcg ggatattatt ttatcatcac
      661 tttacgctct gctgtcccgt tttctgtttc tgctttcaga aaatataaac ctgcggggag
      721 ttgctctaca ttcaaagcta cctgtgctgt agcattttta gctgctatag tttgtgacaa
      781 cacaagctgt ccgttaacac tataaagact tatgtttagc tgaccatgat aattgttgcc
      841 taaataaacc aacagctcat tgtctgctgg atttggatag gtagtaagct gactaaggct
      901 attgacttca ttaattccaa caggtgctgt aatggtaaat actttgttgt taatatcaaa
      961 aaatatagaa cctgtgctgg caccttcaac ccacattctg caaatatttg atgggttatt
     1021 tggaacagta aaagtatagc tgccattgtt agcaacatta gttgcaattg ttatggggaa
     1081 tgttgttccg ttatttatcg aaaggtaaat gtttacaaac tgttcgttaa atggtgcagc
     1141 atcggtgcca cccacttccc atgtaattgt ttgtgtactt aatgcgggcc atgatatacc
     1201 cgttacattt ggataagtaa ttgcaaaagg tgcagaagtc tgaacaacat ttactgttgt
     1261 aactgtatcg ttataagtta cgccaccacc attgggtttg ttatctcttg cagtaagtct
     1321 gaaatgtaat gagcgtgcat aagatgttat caactcacct ttagcattta cctgattgag
     1381 caatacagac aattttggaa aaagacgaag tgcagaagtt gaaggagcat aactgcgata
     1441 cataggtgca ttacctgttg gcaaactcca tgctcccgca gggcctaagt caaactgctc
     1501 ccaactgaaa gttatagaat caccatcagg gtcgctgcca tagccattta atttaaatgc
     1561 tgtgcctaca ggaacattaa atatgcctcc gccactaaca gtaggtgcat tgttaaacgt
     1621 gatggtattt acaggacaag agttgccagt tgacaaggtt gaataatcaa ctatttcatc
     1681 aaaacttttt gtatgaaaat aagctatact gtgtgcagcc aaatcatcag agccacaaat
     1741 tcctgcataa cccataatgg ttacaccact accgggctca taagctgcac cggcttcgcg
     1801 gttaccctga caacttcctg ttacgctgtt aaaagtgtga ttgcctccaa actgatgccc
     1861 catttcatga gctacataat caatatcaaa cgggtcattg attggtgcag cagatccagt
     1921 tacacctttt gccttatctg cagttttaca gatacaacct aagtaggcaa tacctccacc
     1981 accggtacta aaaacgtgac caatatcgta attgggagta ccaataaaaa agtttacgac
     2041 tgattgattt tctcccagca tggttccgcc actgttattg gtataagggt cggtgttggc
     2101 attaagaaaa attaaagtat cagtgttagc aattaaatta agtcttacat ctacttccga
     2161 ttcataaaca cctgtcacac gatttacaga tgtaaccatc cctgacaacg ctcctgcttt
     2221 tgttccacca tagtatgcag catactcacc ggtgcaggct aatgccaaac gataggtacg
     2281 cagatttgta cctatagaac gattcactga aggcattgag tttgttaagg tcttatttgc
     2341 actttgacct tgttgtaagt cgtcaaactc acaaaccata ctgctctgcg actttgtaaa
     2401 atcttttctg aagtaaacta aataatcaga ggtagtttgc atggaataag ggtcaatata
     2461 aatatcgttc tccgctgttc ttatcaatgc atgaaatccc cattgtgtaa aatctatacg
     2521 aacccatgaa ggttgattgt ttataccgct gcctgagtat gttttaataa atggatattt
     2581 agctgccagt ttgggttgca taacaggcga ctctaccata gaaaaactat tccaactacc
     2641 atcgggcata ggtaatgtaa tcacaaccgg tgattgtttt gctgccatgg taaattccat
     2701 tggcgcattg agcagttggt ttttcagccc ttgtacatca agagtataaa ccttgtattt
     2761 atcaggaaca atctgacgtt tgcctgataa catttcttga gtttctgact tagcagtcca
     2821 aaaactactt gccaacgatt gtgtccctga cagaattaca aataatgcgc ttagtgttaa
     2881 aagttttctc atgtttgaat aaattgaagt tgcgaatgta aactaaaggt aggaaaagat
     2941 agcatccggg taacaaataa ttaaattttt acctcataat gatgctgcaa tcccctttgc
     3001 caacagagga aaacacttta aaataatttc gtccatctga tttacgtttg atggaataac
     3061 tgcaatggca tagtttccat tgattagctt tccggtttta tcatagatgg taaaatgaag
     3121 tctgatttct cgctcatata ttttattggc aatatctaag cagtttttat aattggtttt
     3181 aatctcaaat tgagtaagaa aaacaaaaat atctgtatta aatcgctcgc tcaaatcggc
     3241 taatactttt ttattctgaa tatcggcttt catgtagcgg ttgtgtacat ctgtattcag
     3301 ataattggtt gctgtacgcg aatcggtcag tgttttatct gatgttgcat tcatcatact
     3361 ttcgggtgat cgtgtcacag gtgtagcata gtcaaatgct gtttggctga atatatcacc
     3421 tgctgactga atataggcag gcaccgtatc gttttgtaaa agtgaaatag taggataatg
     3481 tctttttagt tcgcgcgagg taaaccattc gctgcgattg tgaaaataat tacggattgt
     3541 tttagggtct tttcgggttt cttctgcaat gtctttatca gcatctgaca aataaaatcg
     3601 tgggtcgtag ggaatgagta atatacgatg ttcaatactg tctttctttt cagtcttatt
     3661 atcctgtgcg ttcacaataa taaaagccaa tagtgatatt gttatacaaa caaatcgttt
     3721 caacataatt ttcttttgtc ttacaaaaat aaagagtggt gttatgcctc atagatgttt
     3781 attgacaact tattaaaatg ctactgttaa ctcaaaaagg tagtataata tgagtagcta
     3841 aggtattctt tgcattaaaa gaaattctct cgctaagaca taaagaaact gaacacgcta
     3901 agagaattcg ccctttgcga tctttgcgtg aaaaaaaaac tacacttgct acaaatactc
     3961 ctttgcgttc tttgcatgaa aaaaaagttt ctcgccaagg cacaaagaaa taaaacacgc
     4021 taaggccact aagaaaaaca actttgccta atttgcgtga aataaaaaaa gtctctcgct
     4081 aagacgcaaa gaaataaaac acgctaaggc cactaagaaa aaaacacctt tgcgagcttt
     4141 gcgtgaaaag aaaactacac ttgctacaaa tactcctttg cgttctttgc atgaaaaaaa
     4201 agttt
//
LOCUS       DAOOOL010000181         4193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2387_length_4193_cov_31.103673, whole genome shotgun sequence.
ACCESSION   DAOOOL010000181 DAOOOL010000000
VERSION     DAOOOL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_4193_cov_31.103673"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PKU82_11010"
     CDS             complement(<1..1226)
                     /locus_tag="PKU82_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOZ83442.1"
                     /translation="MYVIKRDGKKESVKFDKITARIQKLCYGLDPHHVSSLNVAQRVI
                     EGVYDGVTTSELDNLAAEVAATMTVKHPDYALLASRIAISNLHKNTKKSFSETMKDLY
                     TYIDPKTGKQAPLLADDVWAVIEKNAELLDSTIIYDRDFGYDFFGFKTLEKSYLLKLN
                     GKVVERPQHMLMRVSVGIHKEDIDGAIETYNLMSERWFTHATPTLFNAGTPKPQLSSC
                     FLLTMKGDSIDGIYDTLKQTALISQSAGGIGLSIHNVRATGSYIRGTNGTSNGIVPML
                     RVFNDTARYVDQGGGKRKGSFAIYLEPWHADIFEFLDLKKNHGKEELRARDLFYAMWI
                     PDLFMKRVEAEGEWSLFCPNECPGLADTYGAEFEMLYTKYESEGRQRKTIKARELWSA
                     ILESQVETGTPYMLYK"
     gene            complement(1354..2325)
                     /locus_tag="PKU82_11015"
     CDS             complement(1354..2325)
                     /locus_tag="PKU82_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HOZ83443.1"
                     /translation="MMQDELLLRENKERFVLLPINYPKVWEMYKKHEQSFWTAEEIDL
                     HPDLVDWENKLNDDERHFIKHVLAFFAASDGIVNENLAVNFMSEVQIPEARCFYGFQI
                     MMENIHSETYSLLIDAYIKDPKEKHYLFNAIETVPCVKKKADWALRWIQNGSFAERLV
                     AFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSMLQNKM
                     TQEQVHTIIKDAVNCEHEFVTDALPVSLIGMNARLMCQYIEFVADRLLVSLGYSKIYN
                     AANPFDFMEMISLQGKTNFFEKRVGEYKKAGVAQSQESQKIRFDEDF"
     gene            complement(2721..3773)
                     /locus_tag="PKU82_11020"
     CDS             complement(2721..3773)
                     /locus_tag="PKU82_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83444.1"
                     /translation="MINRSNYEVWFIDYADGQLSPDQVAEMLLFLEENPDLKNEFSLF
                     EQVTLPVDTVEFAFKKSLKKTKPSKEVFDEMAVRFLENEISPAELEQYQQWINDFADL
                     KAEHSLFAQIKLHADESVVFRGKNKLKKKVGNVVLLPWLRYAAAACVVAMVLFFANRN
                     TPDNHQQAEHKSVSNPVQHNKPTIKVDVKPDAKNTIALSSNKNSTEPVGIIKHHNQKL
                     EIKESWKNPSIEIALIKTKEATLTSSVNPEIVPAANMENSYTLVMRNTDIQSEKKQDI
                     IKDIALQKFNSFTEDIAGLPVEKGKLKTLAVFGKIVNRLTFRRVNIETTYSDDGKLMA
                     YSVSAGNFNFEHEVVK"
     gene            complement(3776..4186)
                     /locus_tag="PKU82_11025"
     CDS             complement(3776..4186)
                     /locus_tag="PKU82_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOZ83445.1"
                     /translation="MKDEDDARDVVQDTFEKCWRNHENVQYEKAKSYLFTTAHHTMID
                     RIRKSSRQELLNEYHEEPSHSKHYSDLKNVLKLALDKLPSIQKSVILLRDYEGYDYKE
                     IGEITGLSESQVKVYIYRGRVALKEFIGSMSNVI"
BASE COUNT         1254 a          854 c          667 g         1418 t
ORIGIN      
        1 tctttataca acatgtatgg tgtgcccgtc tcaacttgag attcaagaat agcactccac
       61 agttcgcgtg cttttattgt cttacgctga cgaccttcgc tttcatactt tgtgtaaagc
      121 atttcaaact ctgcaccgta ggtgtcggca agacccggac actcattcgg gcaaaacaga
      181 ctccactcac cttctgcctc cacacgtttc ataaacaaat ctggaatcca catggcgtag
      241 aacaaatcac gtgcacgtaa ttcttcctta ccatgatttt ttttcaggtc tagaaattca
      301 aaaatatctg catgccaggg ctcaagataa attgcaaatg aaccttttct ttttccacca
      361 ccctgatcta cataacgggc agtgtcatta aaaacacgca gcattggaac aataccattg
      421 gatgttccgt ttgtacctct gatgtaagaa cctgttgcac gaacattatg tatactcaga
      481 ccaataccac cggcactctg tgaaatgagt gcagtttgtt taagtgtgtc atagattcca
      541 tcaatactgt cacctttcat cgtaagcaaa aagcaacttg aaagttgtgg ctttggtgta
      601 cctgcattaa ataaagttgg tgtagcatgt gtaaaccaac gctcactcat caggttgtag
      661 gtttcaattg ccccatctat atcttcttta tgaataccaa cactcacacg catgagcatg
      721 tgctgcggac gttcaacaac ttttccgttt aattttagca gataggattt ctccaatgtt
      781 ttgaaaccaa aaaaatcata tccaaaatca cgatcataaa taatagtact gtcaagaagc
      841 tctgcatttt tttctatcac agcccaaaca tcatcagcaa gtaatggtgc ttgttttcct
      901 gtcttgggat caatgtaggt gtataaatct ttcatggttt cgctgaaaga tttttttgtg
      961 ttcttatgca ggtttgaaat ggcaatacgt gaagccagca aagcatagtc aggatgcttc
     1021 accgtcatgg ttgcagctac ttctgcagca aggttgtcaa gttctgaagt tgtaacacca
     1081 tcataaacac cttcaattac acgttgtgca acattgagtg aacttacatg atgcggatca
     1141 agtccataac acagtttttg aattcttgct gtgatcttat caaactttac cgactctttt
     1201 ttgccgtctc ttttaattac gtacatgtga tttttcttgt taggtggttg tgttaaattg
     1261 tttgtagatt attgttattt caggtagcat aaattaacag ataaaaaagc cggcagaaaa
     1321 aaatattttc taccggcttt caacagttgg ttgttaaaag tcttcgtcaa acctgatttt
     1381 ctgtgactcc tgtgactgag ccactcctgc ctttttatat tcgcctacac gcttctcaaa
     1441 aaagttggtt ttaccttgta atgagatcat ttccataaaa tcgaaaggat tggcagcatt
     1501 gtaaattttt gaatagccta aagaaactaa taacctgtcg gccacaaatt caatatactg
     1561 acacataagt ctggcattca tacctatcag agaaaccggt aatgcatcgg taacaaattc
     1621 atgttcgcag ttaacagcat cttttataat tgtatgcact tgctcttgtg tcattttatt
     1681 ttgaagcata ctatataata agcatgcaaa atcgcaatgc agaccttcat cacgcgaaat
     1741 caattcatta gagaatgtaa gtccgggcat taaccctctt ttcttcaacc agaaaataga
     1801 acaaaaacta ccggagaaga aaatgccttc aactgcagca aatgcaacta acctttcagc
     1861 aaagcttcca ttctgaatcc aacgtaatgc ccagtcggct tttttcttta cgcaaggaac
     1921 tgtttcgatg gcattaaata aataatgctt ctctttaggg tcctttatat aggcgtcaat
     1981 caataaggag tatgtttcgg agtgaatgtt ctccatcatt atctgaaatc catagaaaca
     2041 acgtgcttca ggaatctgaa cctcactcat aaaatttacg gcaaggtttt cgttcacaat
     2101 gccatcgctt gcagcaaaaa atgcaagcac atgttttata aaatgtcgtt catcgtcatt
     2161 gagtttattt tcccagtcaa ccaaatcggg gtgcaggtca atctcttctg ctgtccagaa
     2221 actttgttca tgttttttat acatctccca aaccttcgga tagttgattg gcaacagcac
     2281 aaagcgttct ttgttttctc ttaaaagtaa ttcgtcttgc atcatatttt tttattggta
     2341 aaaaagttcc tccacagact attttttcat aatctgctat ttatttttaa tcgtaaatga
     2401 attattaatc tgaaatctaa gggtggtaat accgcttgca attccgttta caaatcaagt
     2461 taaaaataac attgaacagt gcttgaattt ttaaacaatc aaacacactt ttgaacaaaa
     2521 aacacgttga aaattttttt gtttgatttt atttgcagaa ttaacttttt tgatttttac
     2581 tctataaatg acagctaata ttttatctga attaaccaaa tattttatga agcaaaagat
     2641 aaaagcgaca tttaaaggta taatgcaatc aatgtcagca aagttgaact aatcaacagt
     2701 tgttcatttc tacagcgtag ttattttact acttcgtgct cgaaattaaa attgcctgca
     2761 ctaactgaat aagccattaa cttgccatca tcagaataag ttgtttcaat atttacccgt
     2821 ctaaaagtaa gccggttaac tatcttgcca aaaactgcaa gtgttttcag tttgcctttc
     2881 tcaacgggta gtccggcaat atcttctgta aatgaattaa acttttgaag ggcaatgtct
     2941 tttatgatat cctgcttttt ttcagattgg atatctgtgt ttctcatcac caatgtatat
     3001 gagttttcca tattagctgc aggcactatt tcaggattaa ctgatgaagt tagtgtagcc
     3061 tccttggttt ttatcaatgc aatttcaatt gatgggttct tccaactttc ttttatttct
     3121 aatttttgat tgtggtgttt tataattcca accggttcag tactattttt atttgatgac
     3181 aaagcaattg tattcttagc atcaggctta acatcaactt ttattgttgg tttattatgt
     3241 tgtacgggat tactaacaga tttatgttcg gcctgttgat gattatcagg tgtatttctg
     3301 tttgcaaaaa acaacaccat tgcaacaaca catgcagcag cagcataacg aagccatggc
     3361 aataaaacca cattgccgac tttcttcttt aacttattct taccccgaaa aacaacactc
     3421 tcatcagcat gtaactttat ctgtgcaaat aaactatgct ctgcctttaa atcagcaaaa
     3481 tcatttatcc attgttgata ttgctctaat tccgctggcg aaatttcatt ctctaaaaaa
     3541 cgaacagcca tttcatcaaa gacttcttta gaaggctttg ttttttttaa acttttctta
     3601 aaagcaaact caaccgtgtc aacaggcaat gttacctgct caaataagct aaactcattt
     3661 tttaagtcag ggttttcttc gagaaaaagc aacatttcgg caacctgatc ggggctgagt
     3721 tgtccgtcag cataatcaat aaaccaaacc tcatagttgc ttctgttaat catctctata
     3781 ttacattact catgctaccg ataaactcct ttaatgctac tctacctctg taaatataaa
     3841 ccttcacctg cgattcgcta agtcctgtta tctctccaat ttctttataa tcataacctt
     3901 catagtcgcg caataaaatc actgactttt gtatggaagg caatttatcc aatgctagtt
     3961 tcaacacatt ttttaagtca gaataatgtt tgctgtgcga aggctcctca tgatactcat
     4021 ttaataattc ctgacggctc gattttctga ttctgtcaat catagtatga tgtgcagtgg
     4081 taaacaaata agattttgcc ttttcatatt gaacattttc atgattgcgc cagcattttt
     4141 caaaggtatc ctgcaccaca tcacgcgcat catcctcatc ttttatatta tac
//
LOCUS       DAOOOL010000182         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2418_length_4154_cov_42.106611, whole genome shotgun sequence.
ACCESSION   DAOOOL010000182 DAOOOL010000000
VERSION     DAOOOL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_4154_cov_42.106611"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..685
                     /locus_tag="PKU82_11030"
     CDS             158..685
                     /locus_tag="PKU82_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOZ83446.1"
                     /translation="MDTISFDKLLLKSAFCCMASDGHIDNREIALIKTMCENSQLFKD
                     FNLEEEINSLVSKINTRGKEFISYYFDLLVKSTLTETEELTLIDFAIQTIKADEQIEY
                     SEIRFFKNIRHRLKISNENILAVFPDIEQFLEEDIITESFLDKITSQYLKSAELPQFE
                     LIILQTSSTEDLDNK"
     gene            693..1229
                     /locus_tag="PKU82_11035"
     CDS             693..1229
                     /locus_tag="PKU82_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOZ83447.1"
                     /translation="MSKRVEAEITCPNCSNQFDFTLYRSIWGEYPENRELVMTNQINV
                     ASCPSCNHSTKLQYPFIYTNANQHFAVWWEPEFDAQIDSDSIGYAKMLGEGNYLATAP
                     RIKSWSEFKNTILKFENGELKGKPGAIGKEMNDQMQGFMKHLKDQNKKKSSGCLGAAL
                     ILFVFSSSLLYGMAKIIF"
     gene            1229..1528
                     /locus_tag="PKU82_11040"
     CDS             1229..1528
                     /locus_tag="PKU82_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83448.1"
                     /translation="MTIVIKISLAVLFFLCLFDMPYGYYQIARFSGLIGFAILAYQAH
                     EQGRQTEMFIYVGLALLFQPFFKIALGREIWNIVDVVLGIGLLISIYMKPKESPR"
     gene            complement(1798..2157)
                     /locus_tag="PKU82_11045"
                     /pseudo
     CDS             complement(1798..2157)
                     /locus_tag="PKU82_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            2511..2876
                     /locus_tag="PKU82_11050"
     CDS             2511..2876
                     /locus_tag="PKU82_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83449.1"
                     /translation="MIIRYTVTVRNYHFNPPSQITKTEYDYYKQLVESNPKAKLNVKL
                     QENPIKKGLKFAVLAPFKAISFLKPGVAEGEIKSAINKSKAAKAENEFYESLKECVIE
                     SESFIEFCELVKRKFPYYQ"
     gene            2937..3302
                     /locus_tag="PKU82_11055"
     CDS             2937..3302
                     /locus_tag="PKU82_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83450.1"
                     /translation="MSDRVKLVRDFNRAAKSAFINGQAGTLLEGKITWGDSSYKHAFS
                     KFMGSGFRIKAMSGNPMARHELIEVAKIILNNEMLVRKLISLGWDTLEVHDNHGINGC
                     KWALKDYGNMGGFLTASTQ"
     gene            3299..3721
                     /locus_tag="PKU82_11060"
     CDS             3299..3721
                     /locus_tag="PKU82_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83451.1"
                     /translation="MKIIFFLLQIDTTNVATLKVSNSIWTSWWFWIAVLEFAIIILLL
                     MRKKQNSNLAFSDLKKNTIKNSKKADIDMDNLMQSIHGSGELYKELSRRCHPDRFVNS
                     PKQKIAEEIFQEISNNRRDFEKLSLLKQRAIDELNINF"
     gene            3734..3976
                     /locus_tag="PKU82_11065"
     CDS             3734..3976
                     /locus_tag="PKU82_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83452.1"
                     /translation="MEDSKDIILILTFILRVVGAIVCSNKAKSLNRSSGSWGFFGFVF
                     PILAMIWIQFMKPIIAWEKNVNIETEKVSGESNIGP"
BASE COUNT         1456 a          701 c          797 g         1200 t
ORIGIN      
        1 aaacaataga atttataatt aaaaatcaaa gaccaacagt tcctctatca gaattaaaaa
       61 aatacgaaga gatgcgaaag aaaattgaag aggaaattga tgagagtaaa tcaaatgaaa
      121 gaccaagaat tggttttaaa tcttaataca aaaaaatatg gacacaatta gctttgacaa
      181 attattgctt aaatcagcat tttgctgtat ggcatctgat gggcatatcg acaatagaga
      241 aattgcccta atcaaaacaa tgtgtgagaa ttcgcaattg ttcaaagact tcaacttgga
      301 agaggaaata aactcccttg taagcaagat taatacaaga ggtaaggaat ttatatccta
      361 ctattttgac ttgttagtca aatccacatt aacggaaaca gaagaattga ctttaattga
      421 ttttgcaatt cagacgataa aagcagatga gcaaattgaa tattctgaaa ttaggttctt
      481 taaaaatatt cgccacagac tgaaaataag taatgaaaat attttagcag ttttccctga
      541 catcgaacag tttttagaag aagacattat aacggaatca tttcttgata aaattacaag
      601 ccaatatttg aaatcggcag aattaccaca gtttgagtta atcatccttc agaccagttc
      661 gaccgaagac ttggataata aataataaaa ttatgagcaa aagagtagaa gccgaaataa
      721 cttgtccaaa ttgtagtaat caatttgatt tcacattgta tcgttctatt tggggggaat
      781 atcctgaaaa cagagaattg gtgatgacaa accaaataaa tgttgcgtct tgtccctctt
      841 gcaatcattc tacaaaactt caatatcctt tcatttatac aaatgctaat cagcatttcg
      901 ctgtatggtg ggaaccagaa tttgatgctc aaatagatag cgatagtata ggatatgcaa
      961 aaatgttggg tgaagggaac taccttgcta ctgctccaag aatcaagagc tggagcgagt
     1021 ttaaaaatac aattttgaaa tttgaaaatg gtgaattgaa aggaaagcca ggtgcaatag
     1081 gcaaagagat gaacgaccaa atgcaaggat ttatgaagca tctaaaagac caaaataaaa
     1141 agaaatcaag cggctgcctt ggcgctgctt taattctatt tgtatttagt tctagtttgt
     1201 tatacggaat ggcaaaaata attttctaat gacaatagta attaaaatca gtctcgcagt
     1261 tttgtttttt ttatgcttat ttgatatgcc ttacggctat tatcaaatag caagattttc
     1321 aggccttatt ggctttgcaa ttttagcata tcaagcacac gaacaaggca gacagacaga
     1381 aatgtttatt tacgttggac ttgctttgtt atttcaaccg ttttttaaaa tcgcacttgg
     1441 cagagagatt tggaacatag ttgacgtagt tttaggaata ggacttttaa tatcaatata
     1501 tatgaaacca aaggaaagcc cacgctaaaa gcaagcacat ttgcaattcg cacaagccag
     1561 cgcacagacc taaaattgca aaagagcttg ctttgccaac gtttaaaaaa aataaaaata
     1621 caccgacaca cagctcgaca aaaacagcag acaatgacgc ggaaattgaa tattgatgag
     1681 gcatacagcg acacggcgga cagaaggaca gcaggtaatc gagtagcccg ccctacgcat
     1741 aattaagtag ggagaggctc tcacaccacc gtaaatgcgg acttcgcata cggcggttca
     1801 tgatgtaaaa gagcggccca tgaatgtttg ttcagggcgg gttttcatgt agtgatcgag
     1861 catagactcg tagccccgat tggctagtcg ttccagtgtg atcgtggtga tcagaatggg
     1921 actttgcact acagcccatc caccttttcg cgtgcggctc catgcgtagg catgatggta
     1981 atcgacgccg agacgcatca ggtttttcct tttgcgttca ggtttcttcc aatctttcca
     2041 gacgcagtaa cgaagtcggt ggcgcaccca tttgtcaagt gctcccattt tacctatcag
     2101 actacctaag agaaagcact ccagccaacc tcgttgaatg gttttgacct gttgaatgta
     2161 actgcaaacg ctcgctgttt agcttacttc agtactcacc taacgaacaa ccactcccgt
     2221 aaaaacatga ccggctttgc tatatgctaa actctgggtc gttactcccg cgttttaggg
     2281 actttcacca gttgggattc ttctttggaa gaactatatt tgacattcga ggcgctcaca
     2341 ccacattgcc aaaatattat tttttgactc tttttatttt tatatttgtt gtaaaaaaat
     2401 aatgcatttg gtaatgctaa acgttatgcg ccatgcttat gcttcgttgc aggcgacatc
     2461 agtaacttga aacattgata aaaataaatt aacagctaaa aaaattacag atgataataa
     2521 gatatactgt aacagtcagg aactatcatt tcaatccgcc aagtcagatt acaaaaactg
     2581 aatacgatta ctacaagcag ttggttgaat caaacccaaa ggcaaagttg aatgtaaaac
     2641 tgcaagagaa tccaattaag aagggattaa aatttgctgt acttgcacct ttcaaagcaa
     2701 tttccttttt aaaaccaggg gttgcagaag gagaaattaa atctgcaata aataaatcaa
     2761 aagctgcgaa agcagaaaac gaattttatg aaagtttaaa agaatgtgtt attgaaagcg
     2821 agtcgttcat tgaattttgc gaattagtca aaagaaaatt tccttattat caataaaccc
     2881 aaaaaacatt ttttatgtgg tataaaataa ttcaaatgat tgattggttt ggcgaaatgt
     2941 cagaccgtgt taaattagtt cgtgatttta atagagctgc aaaaagcgct ttcatcaacg
     3001 gacaagcagg aactttatta gagggaaaaa ttacttgggg tgatagttca tacaagcatg
     3061 cgttttcaaa attcatgggt agtggttttc ggataaaagc tatgtcaggt aaccctatgg
     3121 caagacacga attaattgaa gtcgcaaaaa ttattctaaa taatgaaatg cttgttcgga
     3181 aattaatttc ccttggttgg gacactcttg aagtccacga taatcacggt ataaatggat
     3241 gtaaatgggc attgaaagat tatggaaata tgggtggatt tttaactgca agtacacaat
     3301 gaaaataatt ttctttttat tacaaataga cacgacaaat gttgctactc tgaaagtttc
     3361 aaattctatt tggacatcat ggtggttttg gattgcggta ttagaatttg caataattat
     3421 tttattgctt atgcgcaaaa agcaaaattc aaaccttgcc ttttctgact taaaaaaaaa
     3481 cacaataaag aattccaaaa aagcagatat tgatatggac aacttgatgc aaagcatcca
     3541 tggttcggga gaactctaca aagagttaag tagaagatgc catccggaca gatttgtaaa
     3601 ttcaccgaaa caaaaaatcg cagaagaaat ctttcaagaa atttctaata acagaagaga
     3661 ttttgaaaaa ctttcattac tcaaacaacg agctattgac gaacttaata ttaatttcta
     3721 aaactgattc aaaatggaag acagcaagga cataatatta atattaactt ttattcttag
     3781 ggttgttggt gcaatagttt gttctaacaa agcaaaatct cttaaccgca gttcaggtag
     3841 ttggggtttt tttgggtttg tgtttccaat tcttgcaatg atttggatac agtttatgaa
     3901 accaataata gcttgggaga agaatgttaa tattgaaact gaaaaagtaa gtggcgagag
     3961 taacataggc ccataatcaa gtagcctggc ctacgcacaa ttacgtaagg agaggctctt
     4021 ggcgtagtca ccacaaagtg gcacaccacc cgttcatgct gacctcgcat acggcggttc
     4081 atgatgtaaa agagtagctc atgaatgttt gttcatggca gattatttca agtcgcccag
     4141 ataaaatgta tttt
//
LOCUS       DAOOOL010000183         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2488_length_4085_cov_35.002978, whole genome shotgun sequence.
ACCESSION   DAOOOL010000183 DAOOOL010000000
VERSION     DAOOOL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2488_length_4085_cov_35.002978"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKU82_11070"
     CDS             complement(<1..723)
                     /locus_tag="PKU82_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83453.1"
                     /translation="MKRLMLLATGMFLCLTAVEAQPYKQAGGENNLQLLFAPFGSTPL
                     SLNEGITYRRFLKDGKLAARLSISISSSKSTDVIMQADDTLAFPTTTTVFHAEGGGGY
                     IQLTTGMNPQADLVSKTSGFSFRPGIEKHFEGTDRLSPYIGAEIVFSSSKTKFTRDTI
                     LPTDYTSPVFYDTTSTTVIVSAPWTTKSLSGSSGSKTFGVNLIAGMDYYFSKNLSLGA
                     EFGFGFYSTKYDDLKSDYLDVKN"
     gene            complement(839..2224)
                     /locus_tag="PKU82_11075"
     CDS             complement(839..2224)
                     /locus_tag="PKU82_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83454.1"
                     /translation="MKNRVILFWLFLNICFLFFSCATPEKKESVQQVDSSTVVQTHFT
                     KIKLEPGKLLSNVKSESNSNVSFSVYAPSEYSDTTLLPIIYFFDPHADGNLPLEKYKS
                     IANTFNLILVGCNSIENGMPFEQSQQIANILFSDCQNRLPINTQQQIIAGFSGGSKVA
                     CNLAMNQHFFTGLIACSGALFENGKFDKNMSVVSVAGINDFNYHEMVALEKSVDVTHH
                     AFIETDSTHQWPGTSDMYDATLFTLMQMVKQHKLQMQSSVVENFYHTWLKKINDSANK
                     SSPGKQFDQLQFGIMCFDGLTDVTSLKSKLKEIENSDNLRKWRMRSQNMQYQEKEMQK
                     MLRDAFSNQNLAWWQKEITDLKTAATNSNDKMMAAMSNRLLGFIGIACYSFAKQLVNE
                     NAQESDKVLQIYQTVEPNNSEAWFLSAVYHANNKQPEKAMTDFNKAVQNGFNQTSRIN
                     EYPALQQIVAP"
     gene            complement(2214..2744)
                     /locus_tag="PKU82_11080"
     CDS             complement(2214..2744)
                     /locus_tag="PKU82_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOZ83455.1"
                     /translation="MALIKSVNGIIPKFGKNFYAAENATIVGQVVMGDDCSVWFNAVV
                     RGDVHSITIGNKVNIQDGAIIHCTYQKAPTVIGNNVSIGHNAIVHGCTLEDNVLIGMG
                     AIVMDHAIIKTNAIIAAGSVVLENTVVESGCIYAGVPAKKVKEVSPEHFKDLNERIAN
                     NYVMYSGWFKKDTDEK"
     gene            complement(2815..3939)
                     /locus_tag="PKU82_11085"
     CDS             complement(2815..3939)
                     /locus_tag="PKU82_11085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ83456.1"
                     /translation="MLFLLLIPALYAFLTFRWMIGFNAIADKKQTVQQYQINNFFSIV
                     IAVRNEQANLERLLHSLAELNYKKSDFEIIIVDDHSEDGTATMATQLLDLFKLNGTVV
                     SLQNSKGKKAAIAEGVKMCKAEVIITTDADCIFNPDWLVTINNEFNLSKPDMMLLPVV
                     LQGKGFLSNMQQTESLGLAALTFGSLAIGKPLMCNGANLVFKKEAYLQMNNTEMRHDV
                     ASGDDTFFMLSLFAKNKQSIRALVSGKVVVTSDAQTGLSELISQRIRWASKVKHYKQG
                     YIKTTGLFITLFSLVQYAAFLVIILFPVMSPVGLVSAFTIAVKWICDFIFIQNAASKL
                     NQKFYPGAFILMQLLYPVYTFVIGVLSLSDHYHWKGRQYH"
BASE COUNT         1285 a          768 c          656 g         1376 t
ORIGIN      
        1 attttttaca tctaagtaat cagatttcaa atcatcatat ttagtactgt aaaatccaaa
       61 accaaactca gcacctaatg aaagattttt gctgaaatag taatccatac cggcaatcag
      121 gtttacacca aatgttttgc tgcctgatga accacttaaa gattttgttg tccaaggtgc
      181 agaaacaatt acagttgttg atgtagtatc ataaaaaacc ggtgatgtat aatctgttgg
      241 caatatggtg tcgcgtgtga attttgtttt tgaactgctg aaaacaattt ctgctccaat
      301 ataaggtgat aacctgtctg tgccttcaaa atgtttttct atacccggac ggaaactaaa
      361 ccctgaagtt ttgcttacca aatctgcttg tggattcatc cctgtcgtta attgaatgta
      421 gccacctcca ccttctgcat gaaacacagt tgttgttgtt gggaatgcaa gtgtatcatc
      481 tgcctgcata ataacatcag tgctttttga actgctgata gaaatactca gtcttgctgc
      541 aagttttcca tcttttaaaa atctgcgata agtgattcct tcgttcaatg aaagaggtgt
      601 acttccaaaa ggtgcaaaca atagttggag gttgttttct ccacctgcct gtttgtaagg
      661 ttgagcctca acggctgtca gacacaggaa catgcctgtt gctaataaca ttagtctttt
      721 cattttattt ttcagttttt gtttggtgta aaagtattca ctgcaagtcc gataataaat
      781 tctcatccga tgaattataa acatgtcaat atgaataact cagaaatgaa aattcaaatc
      841 agggcgctac aatttgttgc agagcaggat actcattaat ccttgatgtt tgattaaaac
      901 cattctgtac agctttatta aagtctgtca ttgccttttc gggctgcttg ttgttggcat
      961 gataaactgc tgaaagaaac catgcctctg aattattcgg ttcaactgtc tgatagattt
     1021 gtagaacttt atctgactcc tgtgcatttt cgtttaccaa ttgtttggca aaagaataac
     1081 aggcaatgcc aataaatccc aacaggcggt tactcatggc tgccatcatt ttgtcattac
     1141 tattagtagc tgcagttttt aaatcggtaa tttctttttg ccaccatgcc aggttttgat
     1201 ttgaaaaagc atcgcgtaac atcttctgca tttccttttc ctgatactgc atattctgac
     1261 ttcgcatacg ccacttacgt aggttatcgg agttttcaat ttctttaagt ttacttttta
     1321 gacttgttac atctgtcaat ccatcaaaac acataattcc aaattgtaac tgatcaaatt
     1381 gttttccggg agaagatttg tttgctgaat cgttaatttt ctttaaccag gtatgataaa
     1441 aattttctac aacagaactc tgcatctgta atttatgctg tttaaccatt tgcatcagtg
     1501 taaagagcgt ggcatcatac atatcgcttg tgcccggcca ttgatgtgtg ctgtctgttt
     1561 caataaaagc atggtgtgtt acgtcaacag atttttctaa tgcaaccatt tcatggtaat
     1621 taaaatcatt tatacctgct actgaaacta cactcatgtt cttgtcaaac ttgccatttt
     1681 cgaacaaggc tccagaacag gctatcaatc ctgtaaaaaa atgttgattc attgcaagat
     1741 tgcaagctac tttactaccc cctgaaaatc ctgcaataat ttgttgttgc gtgtttatag
     1801 gcaacctgtt ttgacaatca gaaaaaagaa tgttggcaat ctgttgtgat tgttcaaaag
     1861 gcattccgtt ttcaatactg ttgcaaccaa ccaatatcag attaaaagta ttggcaattg
     1921 atttgtattt ttctaatggc aaatttccat cggcatgagg atcgaaaaaa taaattatag
     1981 gaagtaatgt tgtatcgcta tattcggatg gtgcataaac agaaaaagat acattactgt
     2041 tactctctga cttgacgttg gaaagaagtt ttccgggttc taattttatt ttggtaaaat
     2101 gtgtttgcac tactgttgaa gaatcaactt gttgtactga ttcttttttc tcaggtgttg
     2161 cacatgagaa aaaaagaaaa caaatgttta aaaaaagcca aaaaagtata actctatttt
     2221 tcatccgtgt cttttttaaa ccaaccggaa tacattacat agttattagc aatacgctca
     2281 ttcaaatctt taaaatgttc agggcttact tcttttactt tttttgcagg cactcccgca
     2341 tagatacaac ctgattctac cactgtgttt tccagcacaa cagaacctgc agcaattatt
     2401 gcatttgttt taatgattgc atgatccatt acaattgcac ccataccaat cagcacatta
     2461 tcttcaagtg tacagccatg aacaatggca ttgtgaccaa tagaaacatt atttccaata
     2521 actgttggtg ctttttgata tgtgcaatga ataatagcac catcctgaat atttacttta
     2581 ttgccaattg ttattgaatg tacatcaccc ctaacaacgg cattaaacca cacactgcaa
     2641 tcatcaccca tcaccacctg gccaactatt gtagcatttt cggcagcata aaaattcttt
     2701 ccgaattttg gtataatgcc attaacagat tttatcagtg ccatgaatta tttttcttca
     2761 aaactacaat taccattcaa taaaatgaaa ttgtctgtta acaaatttta ttcttcaatg
     2821 atattgcctt cccttccaat gatagtggtc agacaaactt aaaacaccaa taacaaaagt
     2881 ataaacagga tacaacaact gcattaatat aaaagcaccg ggataaaatt tctgattaag
     2941 ctttgatgct gcattctgaa taaaaataaa atcacaaatc cattttactg caattgtaaa
     3001 tgctgacact aaaccaacgg gactcattac tggaaaaagt attattacaa gaaatgcagc
     3061 atactgtact aaagaaaata gagtaataaa caaaccggtt gttttaatat aaccctgttt
     3121 gtaatgtttc actttagatg cccatctaat tcgttgactt atcaattctg acaatccggt
     3181 ttgagcatca gatgttacta caacttttcc ggaaactaaa gcacgtatgc tttgtttatt
     3241 ctttgcaaac aacgacagca taaaaaatgt atcatctcct gaggctacat catgtcgcat
     3301 ctctgtattg ttcatttgca aataagcttc tttcttaaaa accagattgg cgccattgca
     3361 cattaatggt tttccaatag ctaatgaacc aaaagttaaa gcagctaaac ctaatgattc
     3421 tgtttgttgc atatttgata agaaaccttt tccctgtaag acaacaggca atagcatcat
     3481 gtccggcttt gataaattaa attcattgtt tattgttaca agccaatccg gattgaaaat
     3541 gcaatctgca tcagttgtaa ttatcacttc tgctttacac attttaactc cctctgctat
     3601 agctgccttt ttaccttttg aattttgtag tgaaacgaca gtaccattta gcttgaataa
     3661 atcgagtaac tgtgttgcca ttgttgcagt tccatcttca gaatgatcgt caacaataat
     3721 aatttcaaaa tcagattttt tatagtttag ttctgccaac gaatgtagta atcgttctaa
     3781 gttggcttgt tcatttcgaa cagcaataac aattgaaaaa aaattattga tttgatactg
     3841 ttgaaccgtt tgttttttat cagctattgc attaaagcca atcatccatc tgaacgtcag
     3901 aaaagcatac aatgccggaa tgagtaacaa aaaaagcata ggcaaatgta aggataggaa
     3961 aaattatatt gccactttaa aatttaggca taaaaaaaac cgcattgctg cggttttaaa
     4021 tttttaaaag gaattttatc cgttgagtgc ttctgcacct gccacaatct caagaatttc
     4081 gtttg
//
LOCUS       DAOOOL010000184         4050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2512_length_4050_cov_52.986984, whole genome shotgun sequence.
ACCESSION   DAOOOL010000184 DAOOOL010000000
VERSION     DAOOOL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_4050_cov_52.986984"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1450..2145)
                     /locus_tag="PKU82_11090"
                     /pseudo
     CDS             complement(1450..2145)
                     /locus_tag="PKU82_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            2478..>4050
                     /locus_tag="PKU82_11095"
     CDS             2478..>4050
                     /locus_tag="PKU82_11095"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83457.1"
                     /translation="MKCLTTIIVCIVFFIQATAQNNCSNPLEVTICPSVALFNQTNAG
                     MGDDLAPPCNIVGEDVLYKVNITNGASQIFVQVINCTGPFKVFATRTCGSCQSLGTNL
                     ITTLPNYYFLANASNDTVLYVWVDAATTVTYDISFGSDTNVAFVNIPSTQGNLSFDGS
                     VCATPTFEPTKPFFQLKVNGVYQTAPLFFSPLNVVDTVCITTFFKNTTGVEGVKKFVF
                     DFPASGYLNPQIAPIIPGFYNAGNWVATPSNGGKTWTLQFIDAAGIGKGDFTGTPNSC
                     LAYEFCFTLTPLSNNPLYTNTKVNITSDGFGKGYLGTMHQGCCTSFATICNMNFMGGG
                     AFGGTHSFGFGFNDPGALPVELLSFTAKAEKNRVKLNWSTASEKNNSHFTIEKSVNGN
                     NWSKLATVNGQGNSNSIQNYSYTDNYPVSGTSWYRLMQTDFDGTSETFPAQRVYVKPY
                     HYSIFPNPVKDLLTIQFDSDETPVIQIFNPVGNKVFAPFEWSNGMVKINTSQLPTGLY
                     FLSIETYGIAEKEQIA"
BASE COUNT         1266 a          752 c          706 g         1326 t
ORIGIN      
        1 atcaatcatt aacttattgt ttgtaggagt aaatcgtata gtatgacttc caacatttag
       61 acctgtaaac tgaatagcct gttgtgagca agcatctaaa cgataccatg tccagttagt
      121 gtcagtaaca catccaatag attttgatgc accattgtca acagcaacac ctaaacctgt
      181 tgcagtacca tcagtacttc ttgcacgaac aaacaagtat aagttagcct gcgatgagct
      241 tcttggttga aacacatact ctaatggagt gaaggtggtt tgtgttgaag gaaacacaac
      301 tgcagtagta aagtttgaat aatctcctgc agcagcaccc tgaggttgaa tagtttttaa
      361 actatatgtt gcattggtat tatcatacaa ctctgattca atagtaaaat cttttgacca
      421 gaatgatgga tgcgattttt catgccactc atctaactga taaaatacag gagatgatga
      481 atttgtttta tcgtaaatat aagtacttcc ctgacgtctt actttgaatt ttacttgctg
      541 accatcaaga gaaatagttg caacactttc attctctgca ccaccaacca tacttgtatt
      601 tggctgaatg gttcctgtaa tcgcaaattt agattgtgta ttgtgttttc taactacaac
      661 taacttacgt aagtcaccgg cccagaaagt atatccgggt tttcctgaag gagcactgta
      721 atatgcaggt acatcaccaa gcataacact accattgcgc aagaattctt catagcggct
      781 tgcaacagct tgtgcataac ttggcattgc tgtttgccat gcatagcctg ctggatttgc
      841 aggtggcgga tttggaggat tgtaatttcc tgattcgtta aaataacctg tatagaaaaa
      901 ctcagcacca atcatgttaa gacatttcag caatcccata aactgtgctg gacgcatatt
      961 gttatcttca ttaacatccc aacctgctgc aacaaacgga gagaataaat tatcaccggc
     1021 ctgcaattca taataacgac tgtcaacaat ccactgccag ccatgccatg cagtaatcca
     1081 gttacgccag ttgtgtggcc aacgaggata aaaatccggt gtgctataac gctgactatt
     1141 gatttcagaa ttaactaaac gtgcttcgct gtatttctgg cgatattgag gaaaaccatc
     1201 aataccatac tctgtaaatt ttgtattcga caatgcagga atatccatga ttatatcacg
     1261 gtatgattgt atttcattct ctttaaattt tcttgcctga aaagtctgat aatccaaacc
     1321 ggaagcattc tttgcagcaa caactgcagg gtcggctgat aatgcagcat ctgtactcca
     1381 tgcaaagact tcaccattct cattaatcat ggttaatggt cttgtcaatg cattcaatac
     1441 atcctgaaat ttaagtgact caggcatttt ttctattgca taacgcaatg cagttctcgg
     1501 cattttggat ttatgtaaca taacaaaatc aaacacatct ttttcataat gtttacatgc
     1561 agccttcagc atccatccat agcctttctg tactaaatca tcatcatcgg tcagtaagat
     1621 atttgcaatg tcaaagatta aaggaagaaa taatcccttt tttgccggaa ttatcagaga
     1681 tacagcagca gcacgtctca gccaacggtt tgatgataca gcccatttct tcaataactc
     1741 agttttttca ggatacatca tcagataatc tcctactgtg tgattgcaga aagtatcaca
     1801 ggatgcccag ttgttgacat agttatttat ccaactttca aaaaccttaa tgtcaccaac
     1861 ttcaaatttt ttatgcattg attcagtgaa catacaggca atgacggatt cttccagata
     1921 ggatgattgc cataattctt cacagattac aaatatttct tttttgggca atgtccttac
     1981 ttgctgcaaa tattttttac caattgttcg aactattggt gattttacac catatacttt
     2041 ggccatttca ccgggtttaa aaaaacggga agcaataatg cgcacttctt cgtttgctat
     2101 attctttaat tcttttctta taccttgaat gatttcttta ttcacttttt taatcaatta
     2161 aatattgttg atgtttgatt ttgcaaagta aatagtaatg tgttttcttt tgttgaagaa
     2221 aaataatttt tatagctttc agttcatctg tgaacgattt ttgcgatgtg ctgatagtca
     2281 aaacaccaat tagcaattct caaattgccc gcatttcctt tacagtattt tattaagaaa
     2341 cagaatactt gttgaaaaat ttcattttta atttggtata atctttgaaa cggttggttt
     2401 agacatttat cagaagtgtg ctaaagcaag ttgatattgt ctgcaataag tataactcca
     2461 aagtaatagc caaccaaatg aagtgtctaa ccaccattat tgtttgcata gtatttttta
     2521 tacaagcaac agcacaaaac aattgtagta atcccctgga ggttactatt tgtcctagtg
     2581 tagcattatt taatcagact aatgcaggta tgggagatga ccttgcccca ccatgtaaca
     2641 ttgtcgggga agatgtgctt tataaagtaa acattactaa tggagcaagt caaatttttg
     2701 ttcaagtaat caactgtact ggtccattca aggtatttgc tacaagaact tgtgggtcgt
     2761 gccagtcact gggaaccaat ttaataacaa cattaccgaa ttactatttt ttagctaatg
     2821 cttcaaatga cactgtactt tatgtctggg ttgatgcagc aacaacagtt acttatgata
     2881 tttcatttgg aagcgatacc aatgttgcct ttgtaaatat tccgagtaca caaggtaacc
     2941 tttcatttga tggaagtgtg tgtgctacac ctacctttga gccaacaaaa ccattctttc
     3001 agttgaaagt taacggtgtt tatcaaactg cgccattatt tttctcacca ttgaacgttg
     3061 ttgacactgt ttgcataact acttttttta aaaatactac aggagttgaa ggcgtcaaaa
     3121 aatttgtatt tgattttcct gcatcaggat atcttaatcc tcagattgca ccaataattc
     3181 ccggatttta caatgcaggt aattgggtag caactccatc aaatggcggt aagacctgga
     3241 cattgcaatt tatagatgca gcaggcattg gtaaaggtga ttttacaggt acacctaatt
     3301 cttgtttagc ttatgagttc tgttttacat taacaccact ttcaaacaac cctctatata
     3361 caaatacaaa agttaatatt acttcagatg gttttggtaa aggttattta ggaactatgc
     3421 atcaaggttg ttgtacttca ttcgcaacaa tatgtaatat gaattttatg ggaggagggg
     3481 cttttggtgg aacacatagc tttggatttg ggttcaatga tccgggtgca ttacctgttg
     3541 aattattatc ttttactgca aaagcagaaa aaaacagagt gaagctcaac tggtcaactg
     3601 cttctgaaaa aaataacagt cattttacca ttgagaaatc tgttaatggg aacaattggt
     3661 caaaattagc aacggtaaat ggacagggaa atagcaattc aatacaaaat tacagttata
     3721 ctgataacta cccggttagc ggaacatcat ggtacagatt aatgcaaaca gattttgatg
     3781 gcacatcaga aacatttcct gcacaaagag tttacgtaaa gccttatcat tattctatat
     3841 ttcccaatcc tgtaaaagat ttattgacta ttcagtttga ctctgatgaa acacccgtaa
     3901 tacaaatatt taatccggtt ggcaataaag tttttgcccc ttttgaatgg agtaatggta
     3961 tggttaaaat taatacatcc caacttccaa cgggtttgta ttttttgagt attgaaactt
     4021 atggtatagc agaaaaagag caaatagctg
//
LOCUS       DAOOOL010000185         3970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2584_length_3970_cov_63.387484, whole genome shotgun sequence.
ACCESSION   DAOOOL010000185 DAOOOL010000000
VERSION     DAOOOL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2584_length_3970_cov_63.387484"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKU82_11100"
     CDS             <1..785
                     /locus_tag="PKU82_11100"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOZ83458.1"
                     /translation="IDKTPAGLEANLNFIAKKHKNNISSYCDYVFKKTVFADQKRTES
                     FIQSFTPNKLSKLLNDPAYTLIKQCSLAYASKVLPEMTKMTLEINNLQKVYMKGQIEM
                     AAENKKNIYPDANFTLRITYGKVEGYEPRDGIAYKYYTTLDGVAEKYDSTNYDFNVPE
                     KLLELHKKKDYGRYATDGKLVPCFLASNHTTGGNSGSPVLNGQGQLIGINFDRVWEGT
                     MSDIMFDPAKCRNITLDIRYVLFIVDKFAGAKWLTEEMNIVQ"
     gene            complement(789..2546)
                     /locus_tag="PKU82_11105"
     CDS             complement(789..2546)
                     /locus_tag="PKU82_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83459.1"
                     /translation="MKRILLSLAFTITGFLAFAQCSIDSTQTDPGIYPDTLLPATVNQ
                     PYTQDITFVMITDTMGITITNFNIANITGLPVGMTWQCNNFANGCNYDPSQSIYGCVN
                     LSGTPLIAGVYNATVTVIATLQVLGDQTISYTLPFTVLPDTVSNPGFSMTNSSGCLPL
                     SVTFVTNNPGQASYLWDFGNGIQSTLENPPIQTYNAPGDYVVTQTVTANGSPQYFLTD
                     ITVASIPDNYGAPVDVPDMYFYLYDSQGNQIYDSHPSVSNTNAPLSWTLPNIPLQNNN
                     YTLHVWDEDGGLFGADDDLGSVTFPGWSASGTATGTLSGVSGSLVVNYSIMQVPVATA
                     IHTDTVHVYAVPDTPMVSANIPLILCTGDTVVLTCNDTLQVQWYESGNLLIGETNSDL
                     VVTASGNFSAVVTNAHGCTATSVTISVQVNPNPPKPTFFVNGNTLNCVLTGYMLQWYF
                     NGSALTGETALQLTATQQGTYSVIATDSISGCSSVSDPLVFTPVGIDQLQSVISAILF
                     PNPADKSVVVQLTSGRNVFTTVQLLDISGWLLQQKDFMLQQGSTQLPFDLTDTSKGLY
                     LIKITQKNASKTLRLVISR"
     gene            complement(2636..3229)
                     /locus_tag="PKU82_11110"
     CDS             complement(2636..3229)
                     /locus_tag="PKU82_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOZ83460.1"
                     /translation="MSYKPNDYYARKAKQENFAARSIYKLEEIDLKHKLFRQRDNVLD
                     LGASPGSWLQYASKKAGEKGKVFGIDLKPVEVKLPNVFTVQDNIETFDLQTLFDRYGF
                     NDKFDVVMSDMAPNTTGSRFTDQARSFDLSMLALQTAKRFLRPGGHFVCKIFDGEDAM
                     TFRDEVKSCFGECFVLRPKSTRNSSKEIFVTGKYFKT"
     gene            3326..3712
                     /locus_tag="PKU82_11115"
     CDS             3326..3712
                     /locus_tag="PKU82_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83461.1"
                     /translation="MKRTFTFLSVATAFCVVTATVITPSCKSSKSTTSTTTAVSVPDK
                     PSYAVHVKPIVDAACGTKCHSPAKKADGLDLSFYEGVKDACEKHKLLASIKHESGVKA
                     MPRMAPQLSKEAIATIEKWINDGYAQ"
     gene            3744..>3970
                     /locus_tag="PKU82_11120"
     CDS             3744..>3970
                     /locus_tag="PKU82_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOZ83462.1"
                     /translation="MVDSLLLKGFRGSTGISVHQLEQTIKLFEEGATIPFIARYRKEK
                     TGGLSDENLLELKKLLKEVEDFNNRKQFIINT"
BASE COUNT         1314 a          759 c          738 g         1159 t
ORIGIN      
        1 atattgacaa aactcctgca ggtcttgaag ccaatcttaa ttttattgct aaaaaacaca
       61 aaaacaatat atcaagttat tgtgattatg tattcaaaaa aaccgtgttt gcagatcaga
      121 aacgaacaga gagttttatt cagtcattta caccaaacaa actcagtaaa ctcctaaatg
      181 accctgcata tactctcatt aaacaatgtt cattggctta tgcttcaaag gtgcttcctg
      241 aaatgacaaa gatgacctta gaaataaata atctgcaaaa agtatatatg aaaggtcaaa
      301 tagaaatggc ggctgagaat aaaaagaaca tctatccgga tgcaaacttt acacttcgca
      361 ttacctatgg caaggttgaa ggttatgagc cacgtgatgg aattgcttac aaatactata
      421 caactcttga tggagttgca gaaaagtacg attcaacaaa ctatgatttt aatgtaccag
      481 aaaaacttct tgaacttcat aaaaagaaag actatggccg ttatgctaca gatggaaaat
      541 tagtcccctg ttttctggca tccaatcaca caacaggagg taattcagga agtcctgttc
      601 tcaatggaca aggtcagtta atcggaatta acttcgatcg cgtttgggaa ggtacaatga
      661 gcgacattat gtttgaccct gcaaaatgca gaaacatcac attagatatt cgctatgttt
      721 tgtttatcgt tgacaaattt gcaggtgcca aatggctaac cgaagaaatg aatattgtgc
      781 agtaacactc aacggctaat cactagccgc aaagttttag aggcattttt ctgtgttatt
      841 ttaatcagat aaagaccttt acttgtatca gttaaatcga atggcagttg agtagagcct
      901 tgttgcagca taaaatcttt ttgttgcaac agccatccgc taatatctaa aagctgaact
      961 gttgtaaaaa catttctacc tgatgtaagt tgcactacaa cacttttatc tgcagggttt
     1021 ggaaataaga ttgctgaaat gacagattga agttgatcta ttccaaccgg agtaaacact
     1081 aatggatctg atacagagct gcatcctgaa atgctatcag tagcaattac agaataagtg
     1141 ccttgttgag ttgctgttaa ttgcaatgca gtttcaccgg tcaatgctga accattaaaa
     1201 taccactgta acatataacc ggttagcacg cagtttaaag tattaccatt gacaaaaaac
     1261 gttggttttg gtggattagg attcacctga acactaatag ttactgaggt tgctgtgcaa
     1321 ccatgagcat ttgtaacaac agcagaaaaa ttacctgatg cagttacaac taagtctgaa
     1381 ttggtctccc caatcagcag gttgccactc tcataccatt gaacctgcaa tgtatcatta
     1441 caagtcaaca caacagtatc gcctgtacac aaaatcaatg gaatgttagc acttaccatc
     1501 ggtgtatcag gaactgcgta aacatgcaca gtgtctgtat gaatagctgt agcaacaggt
     1561 acctgcatta ttgaataatt cactactaac gaaccggaaa caccagacaa tgtacctgtg
     1621 gctgttccac tggcactcca tcccggaaag gttacggagc ccaaatcatc atctgcacca
     1681 aacaatccac catcttcatc ccatacatgc aaggtgtaat tgttattttg taaaggtatg
     1741 ttgggcaatg tccaagataa gggtgcattt gtatttgaaa cagaaggatg gctatcgtaa
     1801 atctgattgc cttgagaatc gtacagataa aaatacatat caggaacatc aaccggtgca
     1861 ccataattgt cggggatact ggctacggta atatcagtca gaaaatattg tggtgaacca
     1921 ttggctgtaa cagtctgtgt tactacgtaa tcgccaggtg cgttatatgt ctgtatcggt
     1981 ggattttcca gtgtactctg aataccattt ccaaaatccc acaaatatga cgcctgaccg
     2041 ggattattgg ttacaaacgt tacactcaat ggcaaacacc cactgctatt ggtcattgaa
     2101 aagcccggat tgctaacagt gtcaggtaaa actgtaaatg gtaatgtgta gctaatagtt
     2161 tgatcaccta aaacttgtaa tgtggcaatg acggtgacag tagcattgta aacaccggca
     2221 attaatggtg ttccgcttaa attaacgcaa ccataaatag actgagaagg atcataatta
     2281 catccattag caaagttgtt gcattgccat gtcataccta ccggtagtcc ggtaatattt
     2341 gcgatgttaa aatttgttat ggttatgccc atggtatcag taatcatcac aaaggtgatg
     2401 tcctgagtgt aaggttgatt cacagtggca ggcaataaag tgtcgggata aattccggga
     2461 tcagtctgtg tactgtcaat gctgcattga gcaaatgcaa ggaaacctgt aatggtaaaa
     2521 gctaaactaa gtaaaattct cttcatatcc atttctttta gttgagtaaa aatagctaat
     2581 gatttgaagt gcaaaaacaa atttgcagaa acttattaat acacttacta aaatatcagg
     2641 ttttaaaata ctttccagtc acaaatattt cttttgagct gttgcgtgtg cttttaggtc
     2701 gtaaaacaaa acattcacca aaacatgatt ttacttcatc gcgaaaggtc attgcatctt
     2761 cgccatcaaa tattttacaa acaaaatgac ctcccggccg cagaaaacgt tttgctgttt
     2821 gaagggcaag catgctaagg tcaaaagaac gagcctgatc ggtaaaacga cttcctgtag
     2881 tatttggagc catatcactc attactacat caaacttatc attaaagcca tagcggtcaa
     2941 acaaggtttg cagatcaaaa gtttctatgt tatcctgaac agtaaacaca ttgggcaatt
     3001 tcacttctac cggcttcagg tctataccaa aaactttacc cttctccccc gctttttttg
     3061 aagcatattg caaccacgat ccgggtgagg cacctaagtc aagtacatta tccctttgtc
     3121 tgaataattt atgctttaaa tctatctctt ctagtttata aatacttcgg gctgcaaagt
     3181 tttcttgctt tgctttacgt gcataataat catttggttt ataggacaca atctgaattt
     3241 tcggtaaaat tacatgagat gagttaatct aactgttttt caattcaaga aaatctatta
     3301 aacttgcaaa acaaatttaa taattatgaa aagaacattc acttttttat ccgttgccac
     3361 agcattttgt gttgttacag ctactgtaat tacgccttcc tgtaagtcat caaaatctac
     3421 aacaagcacc actactgctg tttctgtacc tgacaaacca agctatgcag tgcatgttaa
     3481 accaatagtt gatgcagcct gcggaaccaa atgccacagc ccagcaaaaa aagcagatgg
     3541 acttgacctt agtttctatg aaggtgtgaa agatgcttgt gaaaaacata aacttttagc
     3601 gtctataaag catgaaagtg gtgttaaagc aatgcctcgc atggcaccac aactcagcaa
     3661 agaagctatt gcaaccattg aaaaatggat caatgatggc tatgctcagt aactttttgt
     3721 tttaagattt ttcatctgtt atcttggttg attctttatt gctaaaaggg tttagaggca
     3781 gcaccggtat atcagttcat cagcttgaac aaactataaa gctattcgaa gagggtgcta
     3841 caattccttt tatagctcgc tatagaaaag aaaaaaccgg agggctgagt gatgaaaatc
     3901 tgctggagtt aaaaaagtta ctcaaagaag ttgaggactt taacaacaga aagcaattca
     3961 tcattaatac
//
LOCUS       DAOOOL010000186         3942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2619_length_3942_cov_106.521739, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000186 DAOOOL010000000
VERSION     DAOOOL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2619_length_3942_cov_106.521739"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2973
                     /locus_tag="PKU82_11125"
     CDS             <1..2973
                     /locus_tag="PKU82_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ83463.1"
                     /translation="NGSSYNNITNYSNLYAGSYTLKIKDANNCEVQRAVSLSNILAPK
                     LIGSVLKQVKCFNANDGEIKILASGGTGVLTYSIDNGITTQISNTFSNLTAGTYSASV
                     IDQNNCRSDTTITLLQPDSLQLLLNITPETCSAGNGSITVSSTGGTSPYLFSSDSGQT
                     FYNVAGFAQLDSNIYYIIVKDSKNCRTLNQVNITNLTGPQIVSLTTTNVSCYGLQDGA
                     VQIATAGGNGTLSFSTNNFITQQTDTFFNALLPGNYTVYVSDTNNCKSNHFFSISQPT
                     VVQSNIVSTQPSCFGYNNGSLIVTANGGTPPYNILWSSGSTDFNLSGIVSGQYSFIIT
                     DAHNCNKSDSIYLSSPTVLAANHTTQNGTCSGNANGSALVSVTGGTPPYQYNWSPIAG
                     NTNYANALPAGIYTVDVTDKNNCTLTEQIQISTPAPIANTFAKNDVSCFGGSNGMITA
                     NALGGTAPYSYLWSYQQQTNDTILNLPVGSYSVTITDANGCDNFFTEQISSPQQIKPN
                     SIINNVSCNGFNDASVTLNVQGGVAPYSYQWNNGVTQSLNTNLAAGIYVVTVTDANNC
                     NRVKAFTITEPLPILLSIYPVDTICIGQQVLLNAIASGGTGTYNWLWNNQAAASTISV
                     TPSVTTNYFVSVTDSNNCPSKTDSILVIVFPPLGITATGYDTICAGQSAVLSSIANGG
                     NGGPYSYLWSNGSDSATVNVSPSVTTTYTVSITDNCTQMPATAMVPVVVNETPSLDVS
                     PMTEQGCMPLEVQFLNSSTYPQGTIFTWNFGDGQTTDSITPLHIYNADGIYTVSVQAI
                     TPDNCVSNIIISNPIEVYPLPVASFNISPESISILHPEINISDSSLLGSIWFYDFGDG
                     QTSTLQNPNHIYQDTGRFTVIQIVESEFGCRDTSKREAIVEGVFTVYIPNAFTPNNDG
                     SNDYFFANGYGITELTMQIFNRWGNKVFETPSVTGRWNGIDTSGKICPQGVYVYTAKI
                     KDQFGIYHTYNGQVTLIR"
     gene            complement(2966..>3942)
                     /locus_tag="PKU82_11130"
     CDS             complement(2966..>3942)
                     /locus_tag="PKU82_11130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOZ83464.1"
                     /translation="EAKPAAKVVAETPVKTVAAPKVEAKPVTQPVVASESDSRVKASP
                     LARSLAGQKGLDLTMIKGSGDNGRIVKRDVEWFKQGNVVAGSYTSAIRTQESFEETPV
                     SQMRKTIARRLSESKFTAPHFYLTLDIEMSRCMDAREAINAATGSKVSFNDFVIKACA
                     AALQQHPKVNSSWLGDTIRTNHHIHIGVAVAVDEGLLVPVVRFADSKTLSQINAEVKT
                     YAQKAKDKKLQPADWEGNTFTISNLGMFDIEEFTAIINPPDACILAVGAIKAVPVVKD
                     GSVIAGNVMRVTLSCDHRVVDGVTGAKFLQTVKGFLENPVLLLGQASI"
BASE COUNT         1225 a          825 c          706 g         1186 t
ORIGIN      
        1 aatggatctt catataacaa cattacaaat tactcgaatc tttatgcagg aagttatacc
       61 cttaaaatta aagatgctaa taattgcgaa gtgcagcgtg cagtttcact aagcaacatt
      121 cttgcaccaa agcttattgg atctgtatta aagcaggtta aatgttttaa tgccaatgat
      181 ggtgaaataa aaattctggc aagtggagga acaggtgttt taacttacag tattgacaat
      241 gggataacca cacaaatatc aaacacattt agcaacttaa ctgcaggcac gtactccgcc
      301 tctgtaatag atcagaacaa ttgcagaagc gatacaacca ttactttatt gcagcctgac
      361 tctttacaac tgttattaaa cattactcct gaaacatgtt cagccggtaa tggaagtatt
      421 actgtttcat caacaggagg cacttctcca tatttatttt catcagacag cggacaaaca
      481 ttttataatg tagcaggatt tgcacagctc gacagcaata tttattacat aatagttaaa
      541 gattcaaaga actgtcgcac tttaaatcag gttaatatta caaacctaac cgggcctcaa
      601 atcgtttctt taacaacaac gaatgtttca tgctatggtt tgcaggatgg tgcagtgcaa
      661 attgcaactg caggtggaaa tggcacttta agtttttcaa caaataattt tattacacaa
      721 cagacagaca ctttctttaa tgctttactt ccgggtaact atactgttta tgtaagtgat
      781 actaacaatt gcaaaagcaa tcatttcttt tctataagtc agcctacggt tgtacaaagt
      841 aacattgtct caacacaacc ttcctgtttc ggatacaata atggctcact cattgtaaca
      901 gcaaatggtg gaacaccacc ttataatatt ttgtggtcgt caggaagcac tgacttcaat
      961 ctttcaggta ttgtatccgg gcaatactcg tttattataa cagatgcaca taactgcaac
     1021 aaatcggaca gcatatattt atcaagtcca acagttttag ctgcaaatca cactacacaa
     1081 aacggaacat gttcaggcaa tgcaaatggc tctgcattag tgtctgtaac aggtggcaca
     1141 ccaccctatc aatacaactg gtcgccaatt gcaggaaaca ccaactacgc caatgcccta
     1201 cccgctggca tatacactgt tgatgttact gataaaaata attgtactct gactgaacaa
     1261 attcaaattt ctacacctgc accaattgca aatacttttg caaaaaatga tgtttcatgc
     1321 tttggaggaa gcaatggaat gataacagca aatgccttag ggggaactgc accttacagc
     1381 tatttgtgga gctatcagca acaaacaaat gataccatcc taaacctgcc tgtaggcagc
     1441 tattcagtga ccatcacaga tgccaacggt tgcgataatt tttttacaga acaaatttct
     1501 tcaccacaac agattaaacc taattcaata attaacaatg tttcatgtaa tggattcaat
     1561 gatgcatctg ttacactaaa cgttcaggga ggtgttgctc cttatagtta tcaatggaac
     1621 aatggagtta cacaatcatt aaatacgaat cttgcagcag gaatttatgt agttactgtt
     1681 acagatgcaa ataactgcaa cagagttaaa gcgtttacaa taactgaacc actgccaatt
     1741 ttactttcaa tttatcctgt ggacactatc tgtattggtc agcaggtatt gttaaatgca
     1801 atagcatctg gtgggacagg aacctacaat tggctttgga ataaccaagc cgctgcatca
     1861 acaatttcag tcactccatc agtaacaaca aactattttg tttctgttac agacagcaac
     1921 aactgcccat ctaaaactga tagtatttta gtaattgtat ttcctccgtt aggaattact
     1981 gcaacagggt atgatacaat ttgcgcagga cagtctgctg ttctgtcatc aattgctaat
     2041 ggtggaaatg gtggaccgta tagttatctc tggtcaaatg gaagtgatag cgcaactgta
     2101 aatgtgtcac catcggttac aacaacatat actgtaagca ttacagacaa ctgtacgcaa
     2161 atgccggcaa cagcaatggt accggttgtg gtaaacgaaa caccctcact tgatgtttct
     2221 ccgatgactg agcagggttg tatgccattg gaagtgcaat tcctcaatag ctccacctat
     2281 ccacagggga caatatttac ctggaatttt ggcgatggtc aaacaactga ctccattaca
     2341 ccattacata tttacaacgc tgacggcatt tacacagtaa gcgttcaggc aataactcct
     2401 gataattgtg taagcaatat tatcatttcg aatccaattg aagtttatcc gttgccggtt
     2461 gcctcattca acataagccc tgaaagtatt tctattttac atcctgaaat aaatatttct
     2521 gacagtagtt tgcttggctc catctggttt tatgattttg gagacggaca aacttcaact
     2581 ttgcaaaatc ctaatcacat ctatcaggat accggaaggt ttaccgtcat tcaaattgta
     2641 gaaagtgagt ttggctgtcg cgacacttca aaaagagagg ctattgttga aggtgttttt
     2701 actgtttata tcccaaatgc atttacgcca aataatgatg gcagtaacga ttatttcttt
     2761 gccaacggtt acggcattac cgaactgaca atgcagatat ttaaccgttg gggaaataaa
     2821 gtttttgaaa cgccatcagt aaccggccga tggaatggca ttgacacttc aggtaaaatt
     2881 tgtccgcaag gtgtttatgt ttatacggcc aaaatcaaag atcagtttgg catatatcac
     2941 acctacaatg gtcaggttac gttaatcaga tagacgcttg ccctaaaagc aatacaggat
     3001 tttctaagaa tccttttaca gtctgcaaaa acttagctcc tgtaacacca tcaactacac
     3061 gatgatcgca gctaagcgta acacgcatca catttcctgc aataacacta ccatctttca
     3121 caactggaac agctttaatt gcacctactg caagtatgca ggcatctggt ggattgataa
     3181 ttgctgtgaa ctcttcaata tcaaacatgc ccagattaga aattgtaaaa gtatttcctt
     3241 cccagtctgc aggttgtagt tttttatcct ttgctttctg cgcataagtt ttcacttcgg
     3301 cattaatctg tgaaagtgtt tttgaatctg caaaacgtac aacaggcaca agtaagcctt
     3361 catcaactgc cactgcaaca ccaatatgga tgtgatgatt ggttctgata gtatcgccca
     3421 accaagatga gtttacttta ggatgctgtt gcaatgcagc ggcacatgct ttaatcacaa
     3481 aatcattgaa tgaaactttg cttcctgtgg ctgcattaat agcctcgcgc gcatccatac
     3541 atcttgacat ttcgatatct aatgtaagat aaaaatgtgg tgcagtaaat ttactttcgc
     3601 ttaatcttcg tgcaatggtt ttacgcattt gcgaaacagg tgtttcttca aaagactcct
     3661 gtgtgcgtat tgccgaagta taacttcctg caacaacatt gccttgttta aaccattcca
     3721 catcacgttt cacaatcctg ccattatctc cactaccttt tatcattgtt agatcaagac
     3781 ctttctgccc tgcaagtgat cgtgctaatg gtgatgcttt tactctacta tcagattctg
     3841 atgctactac tggttgagta acaggttttg cttctacttt tggtgccgca acagttttta
     3901 ccggagtttc agcaacaacc ttcgctgcag gttttgcctc ag
//
LOCUS       DAOOOL010000187         3884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2690_length_3884_cov_119.308958, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000187 DAOOOL010000000
VERSION     DAOOOL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_3884_cov_119.308958"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PKU82_11135"
     CDS             <1..580
                     /locus_tag="PKU82_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83465.1"
                     /translation="GWCIQPFNTFLPYFIKHNIKFDFSVLGGFYLFSNAQYFDFSKAP
                     QKPIYQFENDITVEQNNGCFTEFNISSIYIPQQIKLFEKLFLKLYYKITGDHSFSRGE
                     GQIVVKIDKALATPQQEGHDILDSRWERIAIELMSAVKQGEYKKYLDANNYMHFISHP
                     KMLTRHNIRMFDKFLKFASDKYNLESDFRKMV"
     gene            613..1716
                     /locus_tag="PKU82_11140"
     CDS             613..1716
                     /locus_tag="PKU82_11140"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83466.1"
                     /translation="MKIGSYIFELLHQHDCVIVPQFGGFVARYKNATIHPVQHLFSPP
                     SKIVAFNSELTTNDGLLANYISQKLLLSYSDSCKLINDFVTLCKAQLEANQRIQIEKI
                     GSLFFDPEKNLQFIPADNENFLTDSFGLSTVQSPAIQRDRPFEIFSNRDAAVQPAPKR
                     TNHSKLILKIAAYTGVAALIAFAYFNPAINSKISKGLATIFPVTTEIDSQANHSSVNN
                     NQEQKSKEDLIYSESPVSEKNIYETEKPTVDSSASQITAPTPEKEIAAVEEEPKPEPI
                     VETKKIAETVPAVSNATRQYHIVGGCFSVYDNALNLDAELKSKGFSSALIGKNKNGLE
                     IVAIASVATKSEAEEALQQAQNSGYKDAWIMRK"
     gene            complement(2383..2907)
                     /locus_tag="PKU82_11145"
     CDS             complement(2383..2907)
                     /locus_tag="PKU82_11145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOZ83467.1"
                     /translation="MITLIAGTNNKNSHTEKLARIYYQAFLDAKVEVNYLSLTLLNQY
                     IINEDMYDKNDSKINQIGDKYFLNSDKLLFVVPEYNGSFPGILKLLIDAMDVKKYLYF
                     KKAAVVGVATGRAGNLRGIDHLTSILQHTKITVMPHILPVSSVHLEIDEAHEFKNEKT
                     KMALQQHLKQFLAF"
     gene            complement(2917..>3884)
                     /locus_tag="PKU82_11150"
     CDS             complement(2917..>3884)
                     /locus_tag="PKU82_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83468.1"
                     /translation="DLILEEGLNVKVVLFPDGDDPDSFARKNSTTQLMEYISKNAVDF
                     IGFKTGLLLKEVANDPVRKAELIRDIVESISRIPDGIKRSLYTSQCAAQMDVPEQVLI
                     TELNKIRRNLYKKDNEHQQAELVEKLIEMEYLPQQTILTETNAWYQEQEIIRLLLNYG
                     DKKFNFQGSEKPTTVREFIIEQILPDGFGFDNEIFAGVFSHLTSMSTTDFLSMLKTEN
                     SSELEKLRSKAHELLMNKYELSDWESKGIYVKTEENNFNEAIVNPINHIKLKRVQILK
                     RKNSLLLKEEASLSDERLVEILSQQKIYEEYIYAFSKLLGIVTLE"
BASE COUNT         1294 a          705 c          614 g         1271 t
ORIGIN      
        1 cggatggtgc attcaaccat tcaatacttt tttaccgtat tttattaaac ataatatcaa
       61 gtttgacttt agtgtattag gtggttttta tctgtttagc aatgcacaat attttgactt
      121 ttcaaaagca ccacaaaaac ctatctatca atttgaaaat gatattacag tagaacaaaa
      181 caatggttgt tttactgagt tcaatatttc gtccatttat attccacaac aaataaagtt
      241 atttgaaaaa cttttcctga aattatatta caagattacg ggtgaccata gtttttctcg
      301 cggtgaaggg cagatagtcg taaaaattga taaagcttta gctacaccgc aacaagaagg
      361 tcacgatata cttgactcaa gatgggaacg tattgcaata gaactgatgt ctgcagtcaa
      421 acaaggtgaa tataaaaagt atttagatgc taacaattac atgcacttta tctctcaccc
      481 aaaaatgctt acccgacaca atatcagaat gtttgacaag ttcctaaagt ttgcatcaga
      541 caagtataat ttagagtcag atttcagaaa aatggtctaa ggcataaagt tttttatata
      601 tttgcctgat aaatgaaaat cggcagttat atatttgaac ttctgcatca gcatgattgt
      661 gtgatagttc cacaattcgg aggctttgtt gcgcgttata aaaatgcaac gattcatccg
      721 gttcaacatt tattttctcc tccatcaaaa atagttgcat tcaacagcga attaactaca
      781 aacgatggtc ttctggcaaa ttatatcagt cagaaattat tgctgtcgta cagtgattcc
      841 tgtaagctga ttaatgattt tgtgacttta tgtaaagcgc agcttgaagc caaccaacgt
      901 attcaaattg aaaaaatcgg gtctttgttt tttgatccgg aaaaaaactt gcagtttatc
      961 ccggctgaca atgaaaactt tttgaccgat tcatttggat taagtactgt tcagtcacct
     1021 gccattcagc gcgacaggcc atttgaaatt ttcagcaata gagatgcagc agttcaaccg
     1081 gcaccaaagc gaacaaatca ctctaagttg attttaaaaa ttgctgcata cacaggagtg
     1141 gctgcattaa ttgcatttgc ctattttaat ccggctatca actcaaaaat aagtaaaggt
     1201 ttagcaacaa ttttccctgt tacaactgaa attgactcac aagcaaatca ttcttctgtt
     1261 aacaacaacc aagagcaaaa atccaaagag gatttaattt attctgaatc acctgtaagc
     1321 gaaaaaaaca tttacgaaac cgaaaaacca acagtagatt caagtgcaag tcagataact
     1381 gctcctacac ctgaaaaaga aattgctgct gttgaagaag aacctaagcc tgaacctatt
     1441 gtagaaacaa aaaaaatagc tgaaaccgta cctgcagttt caaatgctac aagacaatat
     1501 catattgttg gcggatgttt ttcagtttat gataatgcat tgaatttaga tgctgaactt
     1561 aaatcaaaag ggttttcgtc tgcgctaatt ggcaagaata aaaatggttt ggagatagtt
     1621 gctattgcat ctgttgcaac taaaagtgaa gctgaggaag ctttgcaaca ggcacagaat
     1681 tcaggttaca aagatgcctg gattatgcgg aaataaatta gtggaataaa gttcttttat
     1741 ttaagaacaa atctggcttg tgtttaagtt ttaatcccaa caactttacg agacaaaagt
     1801 tgatgttttg gctgcagcca taaaattatc attaatttac caaccaagaa ttgtgatttt
     1861 tataatacat aagtaaatta aggttgccat caattctttt attctgaaac cgaatcctat
     1921 tagacacagg aaacacgctg aaaatctttt tgaaaaaggc taaaattagc ataaatacga
     1981 taatcaacag aaaggcaaat gctcgaacta aagaaaataa atatctcatg ggaacaattc
     2041 ttaattcaat aaaaccaaaa aatataccag ataattatat taaaggatta acattctcat
     2101 tagcaaatac cattgccccg gcaaaccggg gctgatggcc tctttatata gacttatttt
     2161 taggtcggct ttcataattc agttttagta acccggctga taaaacccgg ggttattatc
     2221 tggcgattta ttcaattctc tactttcaaa aggttgatac aatattatac ctgatgataa
     2281 ttatctacaa aacttttagg atgaaatgca aataatgcat caaattttgt ctattttcag
     2341 gatgaaatgc actatagaca caaaccacct tgcttatatg ccttaaaatg ccaaaaactg
     2401 tttcaaatgc tgctgcagtg ccatttttgt tttttcgttc ttgaactcat gtgcttcatc
     2461 tatttcgaga tgtactgaag aaacaggcaa aatatgaggc attacagtga tttttgtatg
     2521 ttgcaaaata gaagtaagat gatcaattcc ccttaggtta cctgcacggc ctgtagctac
     2581 acctactact gctgcttttt tgaagtaaag atatttcttg acatccatag catcaattaa
     2641 tagcttgaga atacccggaa aacttccatt atactcaggc acaacaaaaa gtaatttatc
     2701 tgaatttaaa aaatatttat cacctatctg attaatctta ctgtcgttct tatcatacat
     2761 atcttcatta atgatgtatt gattgagcaa tgtaagtgac aggtaattaa cttcgacttt
     2821 agcatccaaa aatgcctgat aataaattcg ggctaatttc tcagtgtgac tgttcttatt
     2881 atttgttcct gcaattaggg ttatcatttt gcttaattat tcaagtgtaa cgattccaag
     2941 caatttagaa aacgcataaa tatactcttc gtaaattttc tgctgtgata atatttcaac
     3001 taatctttca tcacttaaag atgcttcttc tttcaggagc aaactgtttt ttctttttag
     3061 tatctgcaca cgttttaact tgatatgatt tatcggattc acaatggcct cgttaaaatt
     3121 attttcttcc gtcttaacat atataccttt cgattcccaa tcacttaatt cgtatttatt
     3181 catcagtaat tcgtgtgcct ttgaacgaag tttttccaat tcactgctat tttcagtctt
     3241 cagcatagat aaaaaatctg ttgtactcat agaagtcaaa tgactgaaaa cacctgcaaa
     3301 tatttcgtta tcaaaaccaa aaccatcagg taaaatttgt tctattataa actcacgaac
     3361 cgtagttggc ttttcgctac cctgaaaatt aaatttctta tctccataat tcaacagtaa
     3421 gcgaataatt tcctgttcct gataccaggc atttgtttca gttaaaatgg tttgctgcgg
     3481 caggtactcc atttcaatta acttctcaac caattctgcc tgctgatgtt cattatcttt
     3541 cttgtataag ttcctcctga ttttattcag ctcagtaatt agaacttgtt ccggaacatc
     3601 catctgtgct gcacactggc tggtataaag acttctttta ataccatcag gaattcgtga
     3661 aatgctttct acaatatcgc ggatgagttc tgccttacga acaggatcat tagcaacttc
     3721 ttttaaaagc agaccagttt taaatccaat aaaatcaaca gcatttttgc tgatatattc
     3781 catcaattgt gtggtgctgt tttttcgggc aaatgaatca ggatcatctc catccggaaa
     3841 taaaacaact tttacattaa gcccttcttc aagaataagg tcaa
//
LOCUS       DAOOOL010000188         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2697_length_3881_cov_117.990068, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000188 DAOOOL010000000
VERSION     DAOOOL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_3881_cov_117.990068"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKU82_11155"
     CDS             <1..385
                     /locus_tag="PKU82_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOZ83469.1"
                     /translation="FLHFPDIVLSMATVCGGQNCKADLLHLHNLIIKESDRVSAFQRE
                     AYKLNIKTDFCGGSKLKILEESNLKPSKRILKTYNDHRLVMAFAPLALKTGELFLDDI
                     SGVSKSFPDYFEQMKSLGFNIEEVF"
     gene            complement(386..1840)
                     /locus_tag="PKU82_11160"
     CDS             complement(386..1840)
                     /locus_tag="PKU82_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83470.1"
                     /translation="MAFSLSFTSCTWDKMDEVAPPTDSCSQYPPEITAIISNKCATAG
                     CHNTLSNSAASGLNLTNWASMIKGNDAGAVVIPGNADYSTLFSFCNIYSDLGFTGNEP
                     KMPYNETSLSHSEVSTIKNWIINGAKNACGQDIFPQDDNRKKVYISNQACDQVAVLDE
                     SSGLLMKWFDVGNIAGFTEAPHMIRLTPDEKYFLIAFFSSGIIQRYRTSDDSFVDQVD
                     FSNGMPGQWGTLVISPDSKRAYSVDWSASGRIAVLNLENFPMTSFTSLPIINLAYPHG
                     MAFNSTGDKLYITNQNGSTYYVVDTIGSPSDFPQWLSNIQTYSYGGGLNGHEIIFSPD
                     YSKYFISCDATNEVRVFDANTHTLLANIPVGTLPQEFSLSLKKPYLFVTCTEDIINGK
                     RGSVAVIDYNNLTLIKKIHTGTQPHGVAVDDDKGVVYVANRNVDANGEAPHHSGACAG
                     RNGNLTQIDMNTLELVPGYKCELGVNPYSVAYKK"
     gene            complement(1866..>3881)
                     /locus_tag="PKU82_11165"
     CDS             complement(1866..>3881)
                     /locus_tag="PKU82_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83471.1"
                     /translation="TFINYGSAGDIDYRYDPFYEKLFPQNMTNFCLLVDYDGDGREDI
                     FTYNRSVSGFDVYHNDSNTTGILTFSLQYQGLQTCYLNAGGNCFTTSLYVAPVNQPVL
                     FDINNDGDLDVLTFQISANFIEYHENMAKELFNRTDTLVLSEVNSKWGHMSLSSNANS
                     AILGLPRIPLTSGNADVLSNRHSGTCMIAFDDDGDGDADLLNGDLLGDNLLYLHNGAS
                     NNASDSIDWQDTSFPSNGGPVNYVTFPSAYLLDADNDGITDMIVSSCTESQSENLNNN
                     WYYHNAGSNTSPDFQYVKNKFLTDEMIDVGTASMPVFFDVDADGKTDIVIGNKGYFIT
                     QSGPAHYEAALSWYKNTSTGQCPEFTLVSNNFGSFQQYNYKNIYPAFGDLDGDGDADL
                     LLGNEDGTFHYYTNTAGAGNIPQYTLTTGGFNFQNLDVGSNSTPQLYDINNDGLLDLI
                     SGELFGKISYFQNTGTVNTPIFTLITPTFGNINVTNTAQTFYGYSVPHLFSDNGTLKL
                     LVGSESGDLFLYDSINTTAGSTFHLISNNAYLIHEPPRAAPAMADLNNDSSPEIIVGN
                     QAGGLSFYSNSLPGCPLAINNVLSDYQVNVYPNPANSHLIIDTDDANGLKKQITLTDL
                     SGRIIHSQNTFSRSTLLNTEKFANGIYLITIAAQSNNITKKIIIRHD"
BASE COUNT         1193 a          720 c          731 g         1237 t
ORIGIN      
        1 ttttttacat tttcccgata ttgtattaag tatggcaact gtttgtggtg gacaaaattg
       61 caaagctgat ttactacatc tacataattt aattattaaa gaatctgacc gtgtttctgc
      121 ctttcaaaga gaggcatata agttaaacat caaaacagat ttctgtggtg gttcaaaatt
      181 gaaaatcctt gaagaatcaa acctcaaacc ttcaaagcgt attttaaaaa cctacaacga
      241 tcaccggttg gtaatggctt ttgcaccatt agcattaaag acaggtgagc tttttctaga
      301 tgatatttcc ggtgtctcaa aatcgttccc agattatttt gagcagatga aatctttagg
      361 atttaatata gaagaagttt tttgattatt tcttgtaagc aacggagtat ggatttacac
      421 ccaactcgca tttatatccc ggaaccaact caagcgtgtt catgtctatc tgagttaaat
      481 ttccattacg tcctgcgcat gcgccactat gatgtggtgc ttcaccattt gcatcaacat
      541 ttctgtttgc tacataaaca acacctttgt catcatcaac agcaacacca tgtggctgcg
      601 ttcctgtatg tatctttttg attaaagtta aattattgta atctatcact gcaacagaac
      661 cgcgttttcc attaatgata tcctcagtac atgtaacaaa taaatagggt ttcttcaatg
      721 ataaagaaaa ctcctgtggt aaggtgccta ccggaatatt tgcaagcaat gtatgcgtgt
      781 tggcatcaaa aacacgcact tcattggtgg catcgcatga aataaaatat tttgaataat
      841 caggagagaa aattatttca tggccgttga gtccgcctcc ataagaataa gtctgaatat
      901 tactgagcca ttgcggaaaa tctgatgggc ttccgatagt atccacaaca taataagtgc
      961 ttccattttg attggtgata taaagtttat ctcctgtgct gttaaaagcc ataccatgcg
     1021 ggtaggctaa atttatgata ggtaaggagg tgaatgatgt catcggaaaa ttttcaagat
     1081 tcaacactgc aatcctgcct gatgcactcc agtcaacact ataagcacgt ttactgtccg
     1141 gtgaaataac caaggtgccc cactgcccgg gcataccatt actgaaatca acctgatcta
     1201 caaagctgtc atcagaagtt ctgtagcgtt gtatgattcc tgaactaaaa aaagcaatca
     1261 gaaaatattt ttcatccggt gtaagtctga tcatgtgtgg tgcttcagtg aagcctgcaa
     1321 tatttcctac atcaaaccat ttcatcaata atcctgatga ttcatcaaga actgcaacct
     1381 gatcgcaagc ctgattggag atgtaaactt tttttctgtt gtcgtcttgc ggaaaaatat
     1441 cctgaccaca tgcatttttt gctccattga ttatccagtt ttttatggtt gacacttcgc
     1501 tgtgagaaag tgatgtttca ttgtatggca tctttggttc atttcctgta aatcctaaat
     1561 cagaatagat attgcagaaa gaaaataatg tactgtaatc tgcattgccc ggaatcacta
     1621 cagcacctgc atcattacct ttaatcatag aagcccaatt ggtgagattt agtccgcttg
     1681 cagcagaatt agaaagcgtg ttgtggcatc ctgctgttgc acatttgttg ctgatgatgg
     1741 cagtaatttc cggtggatat tgcgagcaac tatcggttgg tggtgccacc tcatccattt
     1801 tgtcccatgt gcaagatgtg aacgacagcg aaaatgccat cagtgttgta aatagaaaaa
     1861 atctcttagt catgacggat gattattttt tttgtaatat tattggattg tgcagcaata
     1921 gttattaagt aaatgccatt tgcaaatttt tctgtattta ataacgtgct acgagaaaaa
     1981 gtgttttgtg aatgaataat tcttcctgat aaatcagtaa gggtaatttg ttttttcaag
     2041 ccattggcat catcagtatc aattatcaga tgtgagttag caggattggg ataaacatta
     2101 acctgataat ctgacaagac attattaata gcaagtggac atcccggcaa tgaatttgaa
     2161 tagaacgata atccacctgc ctgattgcct acaattattt caggagagct atcattattc
     2221 aggtcggcca ttgcgggtgc agcacgaggt ggttcatgaa ttaagtatgc attattacta
     2281 atcagatgaa aagtgcttcc tgcagttgtg ttgatgctgt cgtataaaaa caaatctcca
     2341 ctttctgaac caacaagaag ctttaaagtt ccattgtcag aaaataaatg tggtacgctg
     2401 tagccataaa atgtctgtgc tgtgttggta acattgatgt tgccgaatgt aggtgttatg
     2461 agagtaaaaa ttggtgtatt aacggttccg gtattctgga agtaacttat cttaccgaat
     2521 aattcgcctg aaattaaatc taagagtcca tcattgttga tgtcataaag ttgtggggta
     2581 ctgttgctac ctacatctaa attctgaaaa ttaaatccgc ctgttgttaa agtatattga
     2641 ggaatgtttc ctgcgcctgc tgtgttggta tagtaatgaa aagtgccgtc ttcattgcca
     2701 agtaataaat cggcatcacc atcaccatca aggtcgccaa aggcaggata aatgtttttg
     2761 taattatatt gctgaaaaga accaaagttg tttgacacca gtgtaaactc aggacactgt
     2821 cctgtgctag tatttttata ccaggataat gcagcttcat agtgtgcagg tccactttgt
     2881 gtgataaaat aacctttgtt gccaataaca atatctgttt ttccatcagc atcaacatca
     2941 aaaaatacag gcattgaagc agtgcctaca tcaatcattt catctgtaag aaatttattt
     3001 ttcacatatt gaaaatcggg agaagtattg cttcctgcat tatgataata ccagttgtta
     3061 ttcagatttt ctgattgcga ctctgtgcag gacgaaacaa tcatatctgt tatgccgtca
     3121 ttatctgcat ctagcaagta ggcacttgga aatgttacgt aatttaccgg gccgccattt
     3181 gacggaaatg atgtatcctg ccagtcaata ctgtctgaag cattatttga agcgccatta
     3241 tgcagataaa gtaagttgtc tcctaataaa tcaccattca gcaaatctgc atcaccatca
     3301 ccatcatcat caaatgcaat catacatgtt cctgaatgac gattggataa aacatcagca
     3361 ttaccacttg ttaaaggaat tcttggtaaa ccaagtatgg cactgttggc attactgctt
     3421 agcgacatgt gtccccattt tgaattgact tcagacaaaa caagtgtatc tgttctgttg
     3481 aataattctt ttgccatgtt ttcatgatac tcaataaagt ttgccgaaat ctgaaatgtc
     3541 aatacatcaa ggtctccatc gttgttgatg tcaaataata caggttgatt taccggtgct
     3601 acataaagac ttgttgtaaa gcaattgcca ccggcattca gataacaagt ttgcaatcct
     3661 tgatattgta aactaaatgt caatattcct gttgtgttag agtcgttgtg atatacatca
     3721 aaacctgaaa ctgaacgatt gtaagtaaaa atatcttcgc gaccatcgcc atcataatca
     3781 acaagaaggc aaaagtttgt catgttttgc ggaaaaagtt tttcgtaaaa aggatcatag
     3841 cgatagtcaa tatcccctgc actgccataa ttgataaaag t
//
LOCUS       DAOOOL010000189         3810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2764_length_3810_cov_31.070306, whole genome shotgun sequence.
ACCESSION   DAOOOL010000189 DAOOOL010000000
VERSION     DAOOOL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_3810_cov_31.070306"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="nusG"
                     /locus_tag="PKU82_11170"
     CDS             complement(<1..529)
                     /gene="nusG"
                     /locus_tag="PKU82_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOZ83472.1"
                     /translation="MTDTGKKWYVIRAISGKEKKVKQYIESEISRLGLSDYVAQVLIP
                     TEKVYQIKDGKKISKERSYLPGYVLIEANLVGEIPHIIENVTGVISFLGSKGEAPAPL
                     RQSEVNRILGKVDELADSEEVLNIPFVVGETVKVTDGPFNSFSGVIEEVHEDKKKLKV
                     MVKIFGRKTPLELSYM"
     gene            complement(541..732)
                     /gene="secE"
                     /locus_tag="PKU82_11175"
     CDS             complement(541..732)
                     /gene="secE"
                     /locus_tag="PKU82_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOZ83473.1"
                     /translation="MNKIRAYVRESYLELTTKVTWPTWAELQNSAIIVLVASLIIGLI
                     IFFMDFLSQGIMNILYKMF"
     gene            complement(835..905)
                     /locus_tag="PKU82_11180"
     tRNA            complement(835..905)
                     /locus_tag="PKU82_11180"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(871..873),aa:Trp,seq:cca)
     gene            complement(1007..2194)
                     /gene="tuf"
                     /locus_tag="PKU82_11185"
     CDS             complement(1007..2194)
                     /gene="tuf"
                     /locus_tag="PKU82_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOZ83474.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLANKGLAEL
                     RSFDSIDNAPEEKERGITINTSHVEYQTENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKIVVFMNKVDMVDDPELLDLVEMEIRELLSF
                     YEFDGNNTPIIRGSALGGLNADAKWVDKIMELMAAVDTYIPIPPRQVDLPFLMPVEDV
                     FSITGRGTVATGRIERGVINSNDTVEIIGMQDKKLTSTVTGVEMFRKILDRGEAGDNV
                     GLLLRGIEKTDIRRGMVVAKPGSITPHTEFKAEIYVLKKEEGGRHTPFHNKYRPQFYL
                     RTTDVTGEIVLPEGREMVMPGDNVTITVKLIVPVAMDKGLRFAIREGGRTVGAGQVTE
                     IIK"
     gene            complement(2414..2485)
                     /locus_tag="PKU82_11190"
     tRNA            complement(2414..2485)
                     /locus_tag="PKU82_11190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2451..2453),aa:Thr,seq:ggt)
     gene            complement(2621..2693)
                     /locus_tag="PKU82_11195"
     tRNA            complement(2621..2693)
                     /locus_tag="PKU82_11195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2658..2660),aa:Gly,seq:tcc)
     gene            complement(2714..2799)
                     /locus_tag="PKU82_11200"
     tRNA            complement(2714..2799)
                     /locus_tag="PKU82_11200"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2763..2765),aa:Tyr,seq:gta)
     gene            complement(2861..2934)
                     /locus_tag="PKU82_11205"
     tRNA            complement(2861..2934)
                     /locus_tag="PKU82_11205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2898..2900),aa:Thr,seq:tgt)
     gene            complement(3287..3763)
                     /gene="purE"
                     /locus_tag="PKU82_11210"
     CDS             complement(3287..3763)
                     /gene="purE"
                     /locus_tag="PKU82_11210"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOZ83475.1"
                     /translation="MKVAIIMGSKSDLEVMQQAAQVLADFQVEHHIQIVSAHRTPELM
                     VQFSKEAYDKDFRVIIAGAGGAAHLPGMVASLTALPVIGVPVKSSNSIDGWDSILSIL
                     QMPNGVPVATVALNAAQNAGLLAIQILATSDNALYRKLEAYKQSLRDKVIRSNEGL"
BASE COUNT         1108 a          846 c          672 g         1184 t
ORIGIN      
        1 gcatataact taactcaagt ggtgtttttc tgccgaaaat tttcaccata accttaagtt
       61 ttttcttgtc ttcatgaacc tcttcaatca cacctgagaa gctgttaaac ggaccatcag
      121 ttactttcac tgtttctcca actacaaacg gaatattcaa cacttcttcc gaatctgcca
      181 gttcatcaac tttacctaat attctgttta cttccgactg acgtaatggt gctggtgcct
      241 cgcctttaga acctaaaaag ctgataacgc ctgttacatt ttctataatg tgaggtattt
      301 ctcctacaag attggcttca atcaacacat aaccgggcag ataacttctt tctttactta
      361 tttttttacc gtctttaatc tgatacactt tttcagttgg gataagcacc tgagcaacat
      421 aatcagaaag gcctaaacga cttatttctg attctatata ttgcttcact ttcttttcct
      481 tgccgctgat agcacgaatc acataccact tctttcctgt atcggtcatc agattcacga
      541 ttaaaacatt ttatataata tattcatgat accttgtgaa aggaagtcca tgaaaaatat
      601 aatcagtccg ataatgagcg aagcaacaag tacaatgatt gcactatttt gcaattcggc
      661 ccatgttggc caggttacct tggttgttaa ttcaagataa gattcccgta cgtatgctct
      721 gattttattc atcagtttta ttgtttaaac tgctttacca gtttctgttc tatttttaaa
      781 acaatacata ttgtcaagta ttgcaatttc tattttacct tcaatcttcc tctgcacgag
      841 tggagagatt cgaactccca tcaacggttt tggagaccgc tattctaccc ttgaactaca
      901 ctcgtaattt atgatagata attaaaccgt tctcctgcga aagccgttgc ttccgtgtat
      961 tggttttcgc cagccccgca taacggggct gactgttcca atacaattat ttaattatct
     1021 cagtaacctg accggcacct acagtacgtc caccttcgcg gatagcgaaa cgaagacctt
     1081 tgtccattgc cacaggaaca attaatttaa ctgtgatagt aacgttatca ccaggcataa
     1141 ccatctcacg tccttcagga agtacgattt caccggttac atcagttgta cgtaagtaga
     1201 actgtggacg gtatttgtta tggaatggtg tgtgacgtcc accttcttct ttcttaagaa
     1261 cgtagatttc tgctttaaac tcagtgtgtg gagtaattga gcctggcttt gcaactacca
     1321 tacctctgcg gatgtcagtt ttttcaatac cgcgtaacaa caaaccaacg ttatcacctg
     1381 cttcacctct gtcaagaatc ttgcggaaca tttccacacc tgtaacagtt gaagtaagct
     1441 ttttatcttg cataccgata atttcaaccg tatcgtttga gttgataaca cctctttcaa
     1501 tacgaccggt agcaacagtt ccacgacctg taattgagaa cacgtcttca accggcataa
     1561 ggaatggaag gtcaacctga cgaggaggaa ttggaatgta ggtatctact gctgccatca
     1621 attccataat tttatctacc catttagcat cagcattcaa tccacctaat gcagaaccac
     1681 gaatgattgg tgtgttgtta ccatcaaatt catagaacga caaaagttcg cggatttcca
     1741 tttcaacaag gtctaacaat tcaggatcgt caaccatgtc aactttattc ataaaaacaa
     1801 caatcttagg tacacctacc tgacgtgcaa gcaggatgtg ctcacgcgtt tgaggcattg
     1861 gtccatcagt tgcagcaact acaagaatgg caccgtccat ctgtgcagca cctgttacca
     1921 tgttctttac atagtcagcg tgaccgggac agtcaacgtg tgcatagtga cggttttctg
     1981 tttggtactc aacgtgcgaa gtattgatag taataccgcg ttctttttct tcaggagcgt
     2041 tgtcgataga atcgaatgaa cgcaattctg ccagaccttt atttgccaac acagttgtga
     2101 ttgcagcggt caaggtggtc tttccgtggt caacgtgacc aatagttcca atatttacgt
     2161 gtggtttgga acgatcaaat ttttctttag ccattttttt atttaataaa caggttactt
     2221 aaataattga tttgtgagat atatataatt aataattgtt aactttaaat tcatttcaaa
     2281 gttgattaat gtgcgtctgt attttctatc tctataattc atagtatttg aaccgttgac
     2341 tcccgatagc tatcggggtt gaactcacac ccatattttt tggggcactc aaatcaacgc
     2401 tcaaacttct ttgagccgtt gatgagaatt gaactcacga cctcttcctt accaaggaag
     2461 tgctctacca ctgagctaca acggcttcta aattccttgt acataattcc caatattagg
     2521 tgagccatta taacttcaat tcgtaagttt caactcgaac ccagaccttt tgagcggaag
     2581 acggtcccga taactatcgg gatcgaaccc agaccttttg agcggaagac ggggctcgaa
     2641 cccgccacct atagcttgga aggctatcgc tctaccaaat gagctacttc cgcttgttat
     2701 ttgccggctt cattggtggg gagtggtgga ttcgaaccac cgaagtcata agacaacaga
     2761 tttacagtct gtcccatttg gccactctgg taactccccg ataatgttca gttttacctt
     2821 agccaattgt tgacaaactg aaaatgtata aataaaaaag agccgatgga gggattcgaa
     2881 cccccgaccc actgattaca aatcagtagc tctggccaac tgagctacat cggcctctta
     2941 aatatcaatg attcccaaac aaattactta tttactaatc tgcttcagaa gttattacgc
     3001 cattaatcca cgatgtcaat gaacacactt tacccttcaa aaaagggaac gcaaaaatag
     3061 aaaaggattt tttattagca aatggttatt gaaaatattt ttaattgtcc ttgaaactat
     3121 tcaatcatca tcttcaatct attgataatg cattagttaa taaagttata tttcaacaat
     3181 ttaaaaactt ataaccaata aaaaaatcag ttggaggtct atatagatgt atttcaatga
     3241 ccggaggctg caatcgaagc tcctgtcagc aaaattttat taaaaatcac agtccttcat
     3301 tggatcggat aaccttatct cttaacgact gcttataggc ctcaagtttt ctataaagtg
     3361 cattatcaga tgtcgctaaa atctgaatgg ctagcaaacc tgcattctgt gcggcattca
     3421 gggcaacggt agccacagga actccgtttg gcatttgcaa tattgaaaga atagagtccc
     3481 atccatcaat actattgctc gactttactg gaacaccaat gacaggaagt gctgttaatg
     3541 atgcaaccat acccggaaga tgagctgctc cccccgcccc tgctatgatg acacgaaaat
     3601 ctttgtcata agcttctttt gagaattgta ccattaactc cggtgtacga tgtgccgaaa
     3661 caatctgaat atgatgttct acctgaaaat cggcaagcac ctgtgctgcc tgctgcatga
     3721 cttcaaggtc tgacttgctg cccataataa ttgctacctt cataataaac cgattgaatt
     3781 ggaatgccac aaaattacat taaataaagc
//
LOCUS       DAOOOL010000190         3769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2805_length_3769_cov_60.353258, whole genome shotgun sequence.
ACCESSION   DAOOOL010000190 DAOOOL010000000
VERSION     DAOOOL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_3769_cov_60.353258"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKU82_11215"
     CDS             <1..874
                     /locus_tag="PKU82_11215"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ83476.1"
                     /translation="EKFPVIDGIPRFVELDNYANAFGLQWKSFAKIQLDSYNGSTISK
                     DRLVRCLGTSLAELQGKTILEVGCGAGRFTEHLVSSGALVHSVDLSAAVEANHDNISD
                     KPNYRIAQADVYKLPFNENSFDIVICLGVIQHTPSPEKTIHALWNMVKPGGTLVIDHY
                     TFDWMYLLKPIMFYRFFLSRMKPEKSKRIVESLVGFFFPLHWKYRSSKLMTMLLNRLS
                     PCYVYIKTFPKMDYDFHFHLTRLDTYDGLTDYYKHLRSKTQIENILKKFKPHSLSVWK
                     GGNGVEARAIKPIG"
     gene            916..1321
                     /gene="tnpA"
                     /locus_tag="PKU82_11220"
                     /pseudo
     CDS             916..1321
                     /gene="tnpA"
                     /locus_tag="PKU82_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1458..2510
                     /locus_tag="PKU82_11225"
     CDS             1458..2510
                     /locus_tag="PKU82_11225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOZ83477.1"
                     /translation="MKKKKLIFVFPNYSTFVQRDIELLQERYDVITFSFNPRNKIFIP
                     LAFIRQFFFLLFYLPSASVVVGQLAAYHTFLPVLMAKLFRKPSVIFLAGTDSAFFPSI
                     QYGNFCRPLLRWFTIASIRMATHLAPKHQSLIHFNYDYDASGAPVQGVKHFIKNFKTP
                     YTAIANGFDSNKFFPLQTERRKNTFITVAVGMGTGNINQLKGVDLILQIAPLFPDCAF
                     TLVGMSTTTLDTLPPNVKALPLQNEKQLQQLYSSHQFYMQLSLSEGFPNAVCEAMLCG
                     CIPVVSNVNALPEIAAEGFVLQHKNKDMLVALIQKALTSDSEKLSKAARLHIIKDYSL
                     EIRKERLFALLNLLKA"
     gene            2599..3321
                     /locus_tag="PKU82_11230"
     CDS             2599..3321
                     /locus_tag="PKU82_11230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ83478.1"
                     /translation="MTKSYKEVAEYYDELWADLEKQSMAGVNSRHRVILNKLKKAGFN
                     KNSTLLEIGCGVGTLSNFLATQVSSGSIDAVDISPASIEYAKKKYSEVKNLSFHVSDM
                     TDFKINKKFDFILFPDVLEHIPVEAHDNIFKTISGLVHANSQVAINLPDPRSIRWLAK
                     NKPELLQIIDQDIEANVLIDKMYSNGFFLFTMETYCLYFTMPDYGWFVFKVKKEYETT
                     EKIAQSTVLLNGIKLRLRNLFS"
     gene            3375..>3769
                     /locus_tag="PKU82_11235"
     CDS             3375..>3769
                     /locus_tag="PKU82_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOZ83479.1"
                     /translation="MKKILIAILLSSFNGYGQQLVWTQKADIPSSTRHSPIGFSINQY
                     GYIATGFNNGTCYNDMWKYDPSTNVWSQVASLPGSGRYGASVFVINSKAYAGIGWSSS
                     NQGLSDFYEYDPIANQWSARANFIGPQIYT"
BASE COUNT         1183 a          617 c          724 g         1245 t
ORIGIN      
        1 agagaaattt cctgttatag atggaattcc gcgttttgtt gagcttgata attatgctaa
       61 cgcttttggg ttacaatgga aatcttttgc taaaattcaa cttgacagtt ataatggcag
      121 cactatttct aaagatcgtt tagtacgatg tttaggtaca tcattagcag agttgcaagg
      181 taaaactata cttgaagttg gttgtggcgc aggacgtttt acagaacatt tggtgagtag
      241 tggtgccttg gttcattctg ttgatttgag tgcagcagta gaagcaaatc atgataacat
      301 tagtgataaa cccaattata gaattgcgca agctgatgtt tacaagctgc cttttaatga
      361 aaatagcttt gatattgtta tttgccttgg tgtgattcaa catacaccat ctcccgaaaa
      421 aacaattcat gcattgtgga acatggtgaa acctggtggt acattggtta ttgatcacta
      481 tacgtttgat tggatgtatt tattgaaacc tatcatgttt tatcgtttct ttctatcaag
      541 aatgaaacct gaaaaatcaa aaagaatagt tgaaagtctt gttggatttt tctttccgct
      601 acactggaaa tacagaagta gtaagttgat gactatgttg cttaatcgtt tgtcgccatg
      661 ttatgtttat attaaaacat ttccgaagat ggactacgat tttcactttc acctgacaag
      721 acttgacacg tatgatggac ttacagatta ttataagcat ctgcgaagta agacacaaat
      781 tgaaaatatt ttaaagaagt ttaagccaca ttccttaagt gtttggaagg gtgggaatgg
      841 tgttgaggca agagcaatta agcctatagg ctgatattcc acaaagtttt atcttcatat
      901 caaaccacat atagaatggc atttgtgaaa attattgtgc atgttgtttg gggtaccaaa
      961 aaacagacat ccttttctca ctaaagaagc acgtaaaaag ctaataaaac atatcagaga
     1021 aaatgccaag gtgaaaaata tttttattga cagaattaat ggccatacag atcatttgca
     1081 ctgtttgatg tggttgaatg ctgatatgag tattgctaaa gcgatgcagt tgataaaggg
     1141 cgaatcggct ttttgggcaa ataaagaaca acttacagaa tcaaaattcg aatgggctaa
     1201 tgaatattat gcagcatcag ttagcgaatc tgctcttgat gcagtaagaa agtatattga
     1261 caatcaggag gagcatcaca agaaaattcc ttttactcag gaatgtgatg agtttctttg
     1321 acctcacact aaagtgtgag gtaccaggca tcgtatagag taaccccatg cttcagcatg
     1381 gggttacagg actaaagtcc tgatgtgagg aagatcccca cctccaaaat ccttactttc
     1441 gcaatcaaat attttccttg aaaaagaaaa aactaatttt cgtttttcct aactattcaa
     1501 cctttgtaca aagggatatt gaattgctgc aagagcgtta cgatgtgatt actttttctt
     1561 ttaatccacg aaataaaatt tttattcctc ttgcattcat caggcagttt ttttttctgt
     1621 tattttatct gccttctgca tctgtagttg ttggtcaact ggctgcctac cacacttttt
     1681 tacctgtgtt gatggcaaag cttttccgca agccatcagt cattttttta gcggggacag
     1741 acagtgcctt ttttccttca atacagtatg gaaatttttg caggccgctg ttgcgatggt
     1801 ttactattgc aagtattcgt atggccacgc atttagcccc caaacatcag tcgctcattc
     1861 atttcaatta cgactatgat gcctccggtg ctcctgtgca gggcgtaaaa cattttataa
     1921 aaaattttaa aacgccatac actgctattg ccaatggttt tgacagtaat aaatttttcc
     1981 ctttacagac agaacgcaga aaaaacacat tcattaccgt tgccgtaggt atgggtacgg
     2041 gcaacattaa tcagctaaaa ggtgttgacc ttattttgca gatagcacca cttttccctg
     2101 actgtgcatt tacactcgtt ggcatgtcga caacgacttt ggatacattg ccaccgaatg
     2161 taaaagcatt gcccttgcaa aacgagaaac aactgcagca gctttattca tcgcatcagt
     2221 tttatatgca gctttcgtta agcgaaggtt ttcctaatgc tgtttgcgaa gccatgctgt
     2281 gtggttgtat tcctgtagtt tctaatgtta atgcactgcc cgaaattgct gctgaaggct
     2341 ttgttctaca gcataaaaat aaagacatgc tggttgcact cattcaaaaa gcactgactt
     2401 ctgattcaga aaaactatcg aaagcagcaa ggctacatat catcaaagac tattctcttg
     2461 aaataaggaa agaaaggctg tttgcattgc tgaatttact taaagcatag acctaactgc
     2521 tttattttag cacaaaggga aaggtttgaa tttttagact attttcgcag gcataattta
     2581 tttttaaatt gaattttaat gactaagagt tataaggaag ttgctgagta ttatgacgaa
     2641 ttatgggcag accttgaaaa gcagtcaatg gctggtgtca atagcagaca cagagtcatt
     2701 ttgaacaagt tgaagaaagc cggattcaat aaaaattcaa ccttattaga aattggttgt
     2761 ggtgttggaa cactctctaa ttttctcgct actcaagtaa gttcaggtag tattgatgca
     2821 gttgatatta gcccggcatc aattgaatat gcaaaaaaga aatacagtga agtgaagaat
     2881 ctttcatttc atgtttctga tatgactgat tttaaaatca ataaaaaatt tgattttatt
     2941 cttttccctg atgtattaga gcatattcct gtagaagcac atgataatat tttcaaaaca
     3001 attagtggtt tagttcatgc taattctcaa gtagccatta atcttcctga cccaagaagt
     3061 attagatggt tggcaaaaaa caaacctgaa ttattacaaa ttatagatca ggatattgag
     3121 gctaatgtgt tgattgataa aatgtattca aatggatttt tcctttttac aatggaaaca
     3181 tattgcttgt acttcacaat gcctgattat gggtggtttg tttttaaagt taagaaggaa
     3241 tatgaaacaa ctgaaaaaat agcacaatct accgtattac ttaatgggat aaaacttcgt
     3301 ttgcggaatt tattttcttg atttattgtt tttaatagtc tttaattata aatttgcttt
     3361 gaagcaatta ttatatgaaa aagattctca ttgcaatttt attatcttca tttaatggtt
     3421 atggccaaca gttagtctgg actcagaaag cagatattcc aagtagtact agacattcac
     3481 caataggatt ttcaattaat cagtatggtt atatagcaac tggttttaat aatggaactt
     3541 gctataacga tatgtggaaa tatgatccaa gcacaaatgt atggtcgcag gttgcttcac
     3601 ttcctggaag tggacgatat ggagctagtg tatttgtcat taatagtaaa gcttatgcag
     3661 gaataggatg gagttcttct aatcaggggt tgagtgattt ttatgaatat gatcccattg
     3721 ctaatcagtg gtcagcgcgc gcaaatttta ttggaccaca aatttatac
//
LOCUS       DAOOOL010000191         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2825_length_3751_cov_58.156115, whole genome shotgun sequence.
ACCESSION   DAOOOL010000191 DAOOOL010000000
VERSION     DAOOOL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2825_length_3751_cov_58.156115"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..66)
                     /locus_tag="PKU82_11240"
     CDS             complement(<1..66)
                     /locus_tag="PKU82_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOZ83480.1"
                     /translation="MSDIASRVKAIIVDKLGVDEGE"
     gene            complement(224..1219)
                     /gene="trpS"
                     /locus_tag="PKU82_11245"
     CDS             complement(224..1219)
                     /gene="trpS"
                     /locus_tag="PKU82_11245"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOZ83481.1"
                     /translation="MDTVVSGIRSTGNLHLGNYFGAIKNFTAMQHEYRCFFFIADYHS
                     LTTHPTPENLQQNVKAALVEYLACGLDPEVCTMYIQSDLPETAELYLLLNMNAYVGEL
                     ERVTSFKEKIRKHPDNINAGLLTYPVLMAADILIHKATKVPVGKDQEQHLEMSRTFGN
                     RFNRLYKTEYFGEPMAFNFGKTLVKVPGLDGAGKMGKSESEGNALFLKDDAATIRKKI
                     MRAVTDSGPTEPNSAKPEAIENLFNLIKLVSSPDTISHFETAYNNCTIRYGDLKKQLA
                     EDMVTFMAPITQKIIDIEKNHDYVKKVVHLGREKAKASAQKTIEEVRQIIGIRRF"
     gene            1314..2585
                     /locus_tag="PKU82_11250"
     CDS             1314..2585
                     /locus_tag="PKU82_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOZ83482.1"
                     /translation="MIPLAERMRPKSLNEYIGQEHLTQAGAVLAKAIEKNTIPSMILW
                     GPPGVGKTTLAFILANSLSRQFYHLSAINSGVKEVRAVIETARQHFETVQQPSILFID
                     EIHRFSKSQQDSLLGAVEKGIITLVGATTENPSFEVISPLLSRCQVYILKALTAEHLR
                     TILHAALTNDAVLKEHKVTIKEDDALLRFSGGDARKLLNLLELVIQVNQQNEICIDNK
                     MVEQTIHQKMALYDKTGEQHYDLISAFIKSLRGSDPNAAVYWLARMIHGGEDPLFIAR
                     RMLILASEDIGNANPNALLIANQCFDAAHKIGFPECRIILSQAAIYLACSPKSNSSYM
                     AIEEALELVAKTGDLPVPLHLRNAPTNLMKEIGYGKDYAYAHSYENNFVKLNYLPDQL
                     KGKKIYSPGKNIREAEFQQFLKIRWENEYDY"
     gene            2680..>3751
                     /gene="creD"
                     /locus_tag="PKU82_11255"
     CDS             2680..>3751
                     /gene="creD"
                     /locus_tag="PKU82_11255"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HOZ83483.1"
                     /translation="MENTSHSFFDRLNQWIKNSVMVKLLSMAILILLLLIPSEMVQSL
                     INERQQTHDSALDEISSKWGFEQHLSGPVINVPYQSFFVDADGKRKSQIQYAHFLPEE
                     LKIAGTLEPEMRHRGIYEAVVYKSDVSLKGYFRFPDFSEWNIAPSDIMWKETTLSLGI
                     SDLRGIKQRLTINLNGNSIEFNPGVENNDVHESGVSIRLPIADSLNKPDFKFDMSFKL
                     NGSKEMYFMPLGKETTVNIKSDWTNPSFDGAFLPDTHHISDKGFDAEWKIFHLNRNYG
                     QKFRGAPTGLQESSFGVKLLIPVDNYQKTYRSAKYAILLITLSFTLFFFIEVLNRKRI
                     HPFQYILVGVALCLFYTLLLSVS"
BASE COUNT         1163 a          685 c          710 g         1193 t
ORIGIN      
        1 ttcaccttcg tcaacaccta gtttgtcaac aataatggct tttactctgg atgcaatgtc
       61 tgacatttta tataaagttt tgaattagag cgcaaattaa atggttttgt aactgatttt
      121 caaaaatatt ttccccaaca taattaattg ttcttggttg aatcagaggc acatacacac
      181 ttatctggca ggtaacaatt ttttaacttt tcaaatcagg gtactaaaac cttctaattc
      241 ctatgatttg ccttacctct tctatcgttt tttgcgcact tgccttggct ttttcgcgtc
      301 caagatgaac cacttttttt acataatcgt ggtttttttc aatgtcaatg attttttggg
      361 ttatgggagc cataaaagtc accatgtctt cggcaagctg ctttttcaga tcaccgtagc
      421 ggatggtgca gttattatat gcagtttcaa aatgacttat tgtatcaggt gatgaaacca
      481 attttatcag attgaaaaga ttttctattg cctcgggctt agccgagtta ggctctgtag
      541 ggccgctgtc agttacagca cgcattatct tttttctgat ggtggctgca tcatctttca
      601 gaaacaaggc attaccttca ctttcagact tacccatttt tcctgcgcca tcaagcccgg
      661 gaacttttac caatgttttc ccaaagttaa atgccatagg ctcaccaaaa tattctgtct
      721 tatataagcg gttaaaacga tttccgaatg tgcggctcat ttcaagatgc tgctcctggt
      781 ctttgccaac aggaaccttt gttgccttgt gaattaatat atcggcagcc attaaaacag
      841 gataagtcaa tagtccggca ttaatattat caggatgttt tcttattttt tctttgaatg
      901 atgttactct ttccaactca cctacataag cattcatatt caacagcaga tagagctctg
      961 cagtttccgg caaatcgctt tgtatgtaca tggtgcaaac ttccggatct aaaccacagg
     1021 caagatattc aaccaatgct gcctttacat tttgctgaag attttcaggt gtaggatgtg
     1081 tggtgagcga gtgataatcg gcaataaaga aaaagcagcg gtattcatgc tgcatggctg
     1141 taaaattttt tattgcacca aaataattac ctaagtgcag gtttcctgtt gatcttattc
     1201 cacttactac tgtatccata ggcgcaaaaa tagattaatt gtattaacag gtttttattt
     1261 tagcataaca gcaaagaatt attatttaat ttgggttata aatattgatt gtaatgatac
     1321 cattagcaga gcgcatgcgg ccaaaatcgc tgaatgaata cattggtcag gaacacctca
     1381 ctcaggcagg agcagttctt gcaaaagcca ttgagaaaaa taccattccc agtatgattt
     1441 tatgggggcc accgggtgtg ggcaaaacaa cactggcttt tatattggct aactctttaa
     1501 gcaggcagtt ttatcatctg agtgcaataa acagtggtgt taaagaagtg cgggcagtaa
     1561 ttgaaactgc aaggcaacat tttgaaacag tacaacagcc atcaatttta tttattgatg
     1621 aaatacaccg ttttagcaaa tcgcagcagg attctttact tggcgctgta gagaaaggaa
     1681 ttattacact ggttggagca acaacagaaa atccatcgtt tgaagtaatc tcacccttgc
     1741 tatcgcgctg ccaggtttat attttaaaag ccctcactgc agaacatctc aggactattt
     1801 tacatgctgc attaacaaac gatgcagtac ttaaagaaca taaagttact attaaagaag
     1861 atgatgcttt actgcgtttc agtggtggcg atgccagaaa acttcttaat ctgctcgaat
     1921 tggtcattca ggtaaatcaa caaaacgaaa tttgtattga caataaaatg gtagagcaaa
     1981 ccattcatca gaagatggcg ctttatgata aaacaggaga acaacattat gatttgattt
     2041 cagcatttat aaaaagcctt agaggtagcg accccaatgc agcagtttac tggctagcaa
     2101 ggatgattca tggtggcgaa gatccgttgt ttattgcaag gcgaatgctc attcttgcat
     2161 cggaagatat tggtaatgca aaccctaatg ctttgcttat tgccaatcaa tgttttgatg
     2221 cagcacataa aattggtttt cctgaatgta gaatcatttt gtcgcaggct gcaatttatc
     2281 tagcctgttc gccaaaaagc aattcaagtt atatggccat tgaagaagct ttggaattgg
     2341 ttgccaaaac aggcgacctg cctgtaccac tgcatctacg aaatgcacct acaaacctga
     2401 tgaaagaaat tggttatgga aaagattatg cttatgcaca tagttatgaa aataattttg
     2461 tgaaattaaa ttatctgccc gaccaattaa aaggcaagaa aatttattca ccagggaaaa
     2521 atattcgcga agcagagttt cagcaatttt taaaaatacg gtgggagaat gaatatgatt
     2581 actgacaaaa aataaattgt ttaataaaag ttaaaatgta tgacatttaa aatatctttg
     2641 tttaaatccg tttaaaagaa aaataaccaa catcatttca tggaaaatac ttcacactct
     2701 tttttcgacc gactcaatca atggataaaa aattctgtca tggtgaaact cctcagcatg
     2761 gcaattttga ttttattatt acttatacca tcagaaatgg tgcaaagtct gattaacgaa
     2821 agacaacaaa cacatgacag tgcattagac gaaatttcat caaagtgggg atttgagcag
     2881 cacctcagcg ggcctgtgat taatgtacct taccaaagtt tttttgttga tgccgatgga
     2941 aaaagaaaat ctcaaattca atatgcacat tttttgcctg aagaattaaa aattgcagga
     3001 acgcttgagc ctgaaatgcg tcatcgcggc atttacgaag ctgtagtgta taagtcagat
     3061 gtttcgttaa aaggatattt tcgttttcct gatttttctg aatggaatat tgccccatca
     3121 gatattatgt ggaaagagac tacgctgtct ttaggcattt ctgatttgcg cggaatcaaa
     3181 caacgtctaa ccatcaacct gaatggaaat tctattgagt ttaatccggg agtagaaaat
     3241 aatgatgtgc atgaatcggg tgtaagcata cgtttaccaa tagccgattc gctcaataaa
     3301 ccggatttta aatttgatat gtcttttaag ctcaacggca gcaaggaaat gtattttatg
     3361 cctttgggaa aagagactac tgttaacatt aaatcggact ggactaatcc gagttttgat
     3421 ggcgcatttc tgcccgacac acatcacatc agcgataaag gctttgatgc agagtggaaa
     3481 atatttcacc ttaacagaaa ctacggacaa aaattcagag gagcgccaac aggattgcaa
     3541 gaatcttctt ttggtgttaa gctgcttatt cctgttgaca attatcagaa aacttaccgc
     3601 tctgccaagt atgctatatt gctaattact ttatcgttta cacttttctt ctttattgaa
     3661 gtgctaaaca gaaaacgtat tcatccgttt cagtacattt tagttggtgt tgctttatgt
     3721 ttgttttata ctttgttgtt gtctgtatcg g
//
LOCUS       DAOOOL010000192         3748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2828_length_3748_cov_86.626320, whole genome shotgun sequence.
ACCESSION   DAOOOL010000192 DAOOOL010000000
VERSION     DAOOOL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_3748_cov_86.626320"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2297)
                     /locus_tag="PKU82_11260"
     CDS             complement(<1..2297)
                     /locus_tag="PKU82_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOZ83484.1"
                     /translation="MKHYYFTFILIIFLSLNHPLKAQVLPPQFTDQLVSTGWDFVEGY
                     TEDSTGQKYVWEKAGLVWVVDTNGVKIPQPLIDISEEVGNWRDHGMNGFAIDPNFRTN
                     GYFYLYYVVDRHYLLHYGTGSYSPTTNEYDAATICRITRYTANSATNFTTTVPGSRLI
                     LLGETKKTGIPVLYDTHSAGTLLFATDGTLIVSAGDGASPATADSGQVASILSYWAQA
                     LADSIITPQQNVGAYRAQLIQSLNGKILRLDPATGDGIPSNPFYDSTDARSPQSRVWA
                     LGLRNPFRISLKPGTGESDPTAGNPGTFYVGDVGWQFWEDINICNSPGMNFGWPVYQG
                     YDLDPNYFAAKQFNFFAPNPLYGSGGCTQPYFTFHELVKNPTANGIVSFDNSCNPGQP
                     IPATVHTFIHARPVIDYYHGNRSRTGIFTGNNASTIDIDDPLSPVDGVRFGGFASVAG
                     PLYQGTKFPLDLQGSYFHADYGGEWIKQFKFNANDTATYVRDFASNMGPVVFLTENKK
                     DGCLNYVKYPDQIRKICYVGNVNNIPIAVALSDTTYGASPLVVQFNGSQSHDPENDSL
                     SFFWSFGDGFTSTLISPQHTYNSPGGVIQSYWAVLTVTDTAGNSAKDSVFISINNTPP
                     VVNITSFNDGDLYSMLQISTLPLQATVTDAEHVPGQLHYKWNVYFHHNQHEHPEPTDT
                     NKITSATILPAGCDGEIYYYRIRLTVTDDGGLSGYSEKSVYPNCTTDCNGVFNGTAFI
                     DSCNTCVGGNTGLQPCTQDCHGDWGG"
     gene            2538..>3748
                     /locus_tag="PKU82_11265"
     CDS             2538..>3748
                     /locus_tag="PKU82_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83485.1"
                     /translation="MKKTFTSSVVMFAVVFFSGNITVQAQSKSSHSHNHAVQMELPFQ
                     AEGSNICIPEILLEDIHYRNMSAATNCLIDSFYYSFYVNSEEQRAYYTYDAMNNLSIL
                     LTQQLSNGSTVWENFNRNTYTYDANKNVTSFLFQQWDNNTSVWKNSTLTTYSYDGNSN
                     RTNYLYQLWDNNTSAWKNSTKISYTYNSGNILTAQQSQQWDNNTSAWKNKTKGIYTYS
                     SNLNLIDYLSQQWDISTTAWKNYTKSTYTYDAHNNQTSNLTQRWDSSSTWKNSIKSTY
                     SYNANNKLISYVYQQWDNTANAWKNGSKSTYSYDANQNLINLVTQLWDNVSSSWKNNA
                     KNIYTYDSNNNKLSDLSQLWSSTSQTWASQYGYLYYYSCAPTSISEITPSSFTVYPNP
                     SNGIFVFETDKV"
BASE COUNT         1246 a          682 c          683 g         1137 t
ORIGIN      
        1 ccaccccagt caccatgaca atcttgtgta caaggttgaa gtcctgtgtt gccaccaaca
       61 caagtgttgc atgaatcaat gaaagcagtg ccattaaaaa caccattaca atcagtcgtg
      121 caatttggat aaacactttt ttcactgtaa ccgctgagcc ctccatcatc agtaactgta
      181 agtctgatgc ggtaataata aatttcacca tcgcagcccg caggtaaaat agttgcagaa
      241 gttattttgt ttgtgtcagt aggttccgga tgttcgtgtt ggttgtgatg aaaataaaca
      301 ttccatttat aatgtagttg accaggtaca tgttcagcat ctgtcactgt tgcctgaagt
      361 ggcaaagttg aaatctgaag catggaatac aagtcaccat cattaaaact tgtgatgttt
      421 acaacaggag gtgtattatt tattgaaata aaaacagaat cttttgcaga attaccagct
      481 gtgtctgtaa cggttaatac agcccaatag ctttgaataa caccgcccgg actattataa
      541 gtatgttgtg gacttatcag agtagatgta aagccatcac caaaactcca gaagaaagat
      601 aaagaatcat tttccggatc gtgtgactga cttccattaa actgaacaac taaaggtgat
      661 gcaccatacg ttgtgtcaga taatgcaaca gctatcggga tgttatttac attaccaaca
      721 taacatattt ttctaatttg atccggatat tttacataat tgagacagcc atccttttta
      781 ttttcagtca gaaaaactac aggtcccata tttgaagcaa aatctcttac ataggtagca
      841 gtgtcgtttg cattaaattt aaattgctta atccattcgc caccgtagtc agcatgaaaa
      901 tagcttcctt gcaaatccag aggaaattta gttccctgat aaagtggtcc tgcaacagaa
      961 gcaaatcctc caaaacgaac gccatctacc ggtgataaag gatcatcaat gtctattgta
     1021 gaagcattgt ttcctgtaaa tattcctgtt cgtgaacgat ttccatgata ataatcaata
     1081 acaggtcgtg catggatgaa agtatgtact gttgcaggaa ttggttgtcc cggattacag
     1141 gagttgtcaa aacttacaat gccattagcc gtgggatttt taaccaattc atgaaaagta
     1201 aaatagggct gtgtgcaacc acctgaacca taaagtggat ttggtgcaaa gaagttaaat
     1261 tgttttgcag caaaataatt tggatctaaa tcataacctt gatataccgg ccatccaaaa
     1321 ttcattccgg ggctattgca gatattaatg tcttcccaaa attgccaacc tacatcacca
     1381 acataaaatg ttccagggtt acctgctgtg gggtctgatt ctcctgtccc cggtttaagt
     1441 gaaatacgaa atggatttct taaccccaat gcccaaactc ttgattgagg agaacgtgca
     1501 tcggtactgt cataaaatgg atttgaaggg attccatcac ctgttgcagg atctaatcgt
     1561 aatatttttc cattcaagga ttgaattagt tgtgcccgat aagcaccaac attttgttgt
     1621 ggtgtgatga tactatctgc taatgcctgc gcccagtaac taagaataga agcaacttgt
     1681 ccgctatctg ctgtagcagg cgaagcaccg tcaccggccg atacaatcaa tgttccgtca
     1741 gtagcgaaca ataatgttcc agcactatgt gtgtcataca aaacagggat tcctgttttc
     1801 ttagtctcac ctagtaaaat gagtctgctt ccgggtactg ttgttgtaaa gtttgttgcg
     1861 gaatttgctg tatatcttgt tatgcgacaa atagtagctg catcatattc atttgttgtt
     1921 gggctatagc tccctgtgcc atagtgtagg agatagtgcc tgtcaacaac atagtataaa
     1981 taaaagtaac cgttagttct gaaattgggg tcaattgcaa aaccattcat tccatgatcg
     2041 cgccaattac ctacttcttc actaatgtca attaaaggtt gtggtatttt aacgccattg
     2101 gtatcaacaa cccaaactaa tcctgctttt tcccaaacat atttttgtcc tgttgaatct
     2161 tcagtataac cttctacaaa gtcccagcct gtgctgacaa gctgatcagt aaattgtgga
     2221 ggaagtacct gtgcttttaa tggatgattg agtgaaagaa aaataattaa tataaaagta
     2281 aagtagtaat gtttcatgat gtcggtttat aacgaaaaag cagtagcaaa tataaatgac
     2341 tgaaaaatat caggcatgta aaatctcttt aagttttcca cttgcaatcc atgagtatga
     2401 agaatgttaa attattctta gtcaacgata aattcgtttt ttaataagtg tacaatagaa
     2461 gtaaatgcaa ccttttttta gaaggtcagt ataaaaggaa gttcaaaact catgtattat
     2521 ttaaatattt aatggaaatg aagaaaacat ttacctcatc tgtagtcatg tttgcagttg
     2581 tatttttttc tggcaatata acagtacaag ctcaaagtaa atcaagtcat agtcataatc
     2641 atgcagtgca aatggagcta ccttttcaag ctgaaggcag taatatttgt attccggaga
     2701 ttcttcttga agatattcat tatcgtaata tgagcgcagc aactaactgt ttgattgata
     2761 gtttttatta ctcattttat gttaactcag aggagcaaag agcatattat acttacgatg
     2821 ctatgaataa tttgtcaatt ctgttgactc agcaattgag taatggctct acagtgtggg
     2881 aaaactttaa tagaaataca tacacttatg atgctaacaa aaatgtgaca agctttttgt
     2941 ttcaacaatg ggataacaat acttctgtat ggaaaaacag tacgcttaca acatacagtt
     3001 atgatggaaa tagcaaccgt acgaactatc tgtatcaact atgggataac aatacatccg
     3061 cttggaaaaa ctctacaaaa atcagttata catataactc gggaaatatc ttgaccgccc
     3121 aacaatcaca gcaatgggat aataacactt cagcatggaa gaataagaca aaaggtatct
     3181 acacatatag tagcaatttg aatctgattg attatttgag ccagcaatgg gatataagca
     3241 ccactgcatg gaaaaattac acaaaatcaa cttataccta tgatgctcat aacaaccaga
     3301 caagcaatct tactcaacga tgggatagct catcaacatg gaagaatagc ataaaatcga
     3361 cttacagtta taatgccaat aataaactaa taagttatgt gtatcaacaa tgggataata
     3421 ctgccaatgc atggaaaaat ggttcaaaga gcacctactc ttatgatgcc aatcagaatc
     3481 tgataaatct tgttacacag ttgtgggata acgttagttc atcgtggaag aataacgcta
     3541 agaatattta cacttatgat tctaataaca ataaattaag cgatttatct cagttatgga
     3601 gtagcacatc tcaaacttgg gcaagtcaat atgggtatct ctattattat agttgtgctc
     3661 cgactagtat atcagaaatt actccaagta gttttacagt ttatcccaat ccatcaaatg
     3721 gaatttttgt atttgaaaca gacaaagt
//
LOCUS       DAOOOL010000193         3715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2862_length_3715_cov_48.505464, whole genome shotgun sequence.
ACCESSION   DAOOOL010000193 DAOOOL010000000
VERSION     DAOOOL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_3715_cov_48.505464"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2218
                     /locus_tag="PKU82_11270"
     CDS             <1..2218
                     /locus_tag="PKU82_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOZ83486.1"
                     /translation="LHIEWIKHQLVKKCIDNNERIETSLLFDIKKDGENKSMPCTVFV
                     MPLFASGRVIGAIEIASLNKLTASGLNYLQRLIEPVSASIYAVYSNALTRELLEESIM
                     QSEELVAQKQELAQVNHNLTIKSEELEVSQKELEKQQEELKAANANLEIKAHLLEERS
                     LAVEEARQSLAFKATQLEQTNKFKSAFLANMSHELRTPLNSILILAKLLAGNKNKNLT
                     DKQIEHASVIHKSGSDLLMLINDILDISKIESGKLEFVFEKMNIETVANDINLLFKEF
                     ANEKQINFQVKVWENCYPEMVSDKLRLEQVIKNLISNALKFTDKNGQVSLDFRKATTE
                     DCKTHNLHFEEDIIAISVTDSGIGIPVEKQKQVFEAFKQADNSTSRKYGGTGLGLTIS
                     REIVQILGGEIVLESTPGKGSTFTVFIPFFASCESTHDTPVKKVITQAETEKQENIKH
                     ESPLISTIVSKFPDDDFTDDRNFITPFDKVILILEKDITFLKTLIDYCHIFNYKAIAS
                     QNGETGILLAKNYQPHVIIVDHNLPDIDGGTLLKNIRSEQKLSHIPIHIISELKPTEG
                     LINEFNITAYHHKPLNKKQLENVMNELQAPVNITKKETVVEHTFHESSLSGKTILMAD
                     DDLRNIYALTTLIENAGANLICAYDGKEAINKLEKNPHVDIVLMDVLMPVMNGVDAIA
                     EIRKTAQFRNIPVLAVLSGSDAQEKEKCLDAGATDTLSRPLNNDQILNKIKFLLYQ"
     gene            complement(2221..3009)
                     /locus_tag="PKU82_11275"
     CDS             complement(2221..3009)
                     /locus_tag="PKU82_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ83487.1"
                     /translation="MNLDLTNKKALVCGSTQGIGKAAAKELCLLGAKVVLLARNEQKL
                     KAVAAELNTFCKTTPEYIVADFSDPENLRQQVRAYINKNGGFNILINNAGGPPAGNIT
                     DAAAESFIQAFKEHLICNHYLALECIAGMKEMGYGRIINVISTSVKQPIKGLGVSNTI
                     RSAVGNWSKTMSIELAPFGITVNNVLPGATLTARLEQIIADKVQKTGKTEKEVIESLT
                     SEIPAKRFANPEETAAAIAFLASPAAGYINGTNVVVDGGRTACL"
     gene            3084..3371
                     /locus_tag="PKU82_11280"
     CDS             3084..3371
                     /locus_tag="PKU82_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83488.1"
                     /translation="MSNPIVDQVSKAANINQEQAAKAIEAVSAALKEKLPYLLHNQID
                     ILLTGGNLSDGVKQKFETLKDDLESSAKDLSTKAQEFTEEVSKKLNNLFQK"
     gene            3389..>3715
                     /gene="nbaC"
                     /locus_tag="PKU82_11285"
     CDS             3389..>3715
                     /gene="nbaC"
                     /locus_tag="PKU82_11285"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HOZ83489.1"
                     /translation="MAVSKPFNLQQWVEENRHLLKPPVGNKQVYIDNKDFIVMIVGGP
                     NSRKDYHFEDAEELFYQIEGDIVVKTIQDGKPVDIPIKQGEMFLLPPRVPHSPQRGAN
                     TIGLVVE"
BASE COUNT         1282 a          684 c          677 g         1072 t
ORIGIN      
        1 cttacatata gagtggatta aacatcagtt ggtaaaaaaa tgtattgaca ataacgaaag
       61 aatagaaaca agtttactct tcgacattaa aaaggacgga gaaaataagt ccatgccttg
      121 tactgttttt gtcatgccac tatttgcatc cggtcgtgtt attggtgcta tagaaattgc
      181 ttctttaaat aaactcactg cttccggatt aaattatctg caacgtctta ttgaacccgt
      241 atctgcaagt atttatgctg tatattcaaa tgcacttact cgcgaactgc ttgaagaaag
      301 tatcatgcag tcagaagagt tggtagcaca aaaacaagag ttggcgcagg taaatcataa
      361 cctgactatt aaatctgaag agttagaggt atcacaaaaa gaattggaaa agcaacagga
      421 agaattgaaa gctgccaatg ccaatcttga aattaaagca catctgcttg aagaaagaag
      481 ccttgccgtt gaagaagcgc gtcaatcact tgcctttaaa gcaacacagc ttgaacaaac
      541 caataagttt aaatcggcat ttcttgcaaa catgtcgcat gagcttcgca caccattaaa
      601 cagcattttg attcttgcca aactgcttgc aggaaataaa aataaaaacc tcaccgacaa
      661 acaaattgaa catgcaagtg tcatacataa gtcggggtct gatctattaa tgcttattaa
      721 tgatattctt gacatttcaa aaattgaaag tggaaagctg gaatttgttt ttgaaaaaat
      781 gaatatcgaa accgttgcta acgacattaa cctgttgttt aaagagtttg ccaacgaaaa
      841 acagataaat tttcaggtaa aagtatggga gaattgttat ccggaaatgg tgagtgataa
      901 gttgcgtctg gagcaagtca taaaaaatct tatctccaat gctttaaagt ttaccgataa
      961 aaacggacaa gtaagtcttg atttcagaaa agcaacaaca gaagattgta agactcataa
     1021 cctgcatttt gaagaagaca ttattgcaat aagcgtaaca gattcaggaa ttggaattcc
     1081 cgttgaaaaa caaaagcagg tgtttgaagc atttaaacag gccgataaca gtacaagcag
     1141 aaagtatggc ggaacagggc tgggacttac catcagtcgc gaaatagttc aaattcttgg
     1201 tggtgaaatt gttttagaga gcacaccggg taaaggaagt acgtttactg tgtttattcc
     1261 gttttttgca tcatgcgaaa gcacacatga cacccctgta aaaaaagtaa tcacacaagc
     1321 agaaacagag aaacaagaaa atataaaaca tgagtcacca ttaatcagta ccatagtttc
     1381 aaaattccct gatgatgatt ttaccgatga cagaaatttt atcacaccct tcgataaggt
     1441 aattcttatt ctcgaaaagg atattacatt ccttaaaaca ttaatagact attgtcacat
     1501 ttttaactat aaagccatag catctcagaa tggtgaaacc ggtattttgc ttgctaagaa
     1561 ttaccaacca catgtcatta ttgtagatca taatctgcct gacattgatg gtggaacctt
     1621 acttaaaaat atacgttcgg agcaaaagct tagtcatatt cctatccata tcatcagtga
     1681 gcttaaacct acagaaggtt taattaatga gtttaatata acggcctatc atcacaagcc
     1741 tttaaacaaa aagcagctcg aaaatgtaat gaatgagtta caagcgcctg taaatattac
     1801 aaagaaagaa acagttgttg agcatacttt tcatgagtca tcattaagcg gaaaaaccat
     1861 tttgatggca gatgatgatt tacgaaatat ctatgcatta actacattaa ttgaaaatgc
     1921 aggagccaat cttatttgtg cttatgacgg taaagaagct ataaacaaac ttgaaaaaaa
     1981 tccgcatgtt gacattgtat taatggatgt cctgatgccg gtaatgaatg gagttgatgc
     2041 tattgccgaa atacgtaaaa cagcgcagtt cagaaatatt cctgtgctgg ctgttctttc
     2101 aggctctgat gctcaggaaa aagaaaaatg tcttgatgcc ggtgctaccg atactttatc
     2161 aagaccatta aacaacgatc agattttaaa taaaattaag tttctacttt atcaataatt
     2221 ttacaggcat gctgtacggc caccatcaac aactacgttg gtcccgttga tataacccgc
     2281 agccggagaa gctaaaaatg caattgcagc ggccgtttct tccggattgg caaatcgttt
     2341 ggcaggaatt tctgatgtca gcgactcaat aacttctttt tctgtctttc ccgtcttctg
     2401 aactttatct gcaataatct gttccagtcg tgcagtgagt gttgcaccgg gcaaaacatt
     2461 gtttactgtt atgccgaatg gagccaattc aattgacata gtcttactcc agttaccaac
     2521 tgctgatcta atcgtatttg aaacacctag tcccttaatg ggttgtttca ctgaggtgga
     2581 gatgacatta ataattcttc cgtaacccat ctctttcatt ccggctatac actccagtgc
     2641 cagataatga ttgcaaatca aatgctcttt aaatgcctga ataaaacttt ctgctgcggc
     2701 atcagtaata tttcctgccg gaggaccacc agcgttgttt atcaaaatgt tgaagccccc
     2761 atttttattt atgtatgcac gaacctgttg tcttaaattt tcaggatcag aaaaatcagc
     2821 cacaatatac tctggcgttg tcttacaaaa agtgtttaat tccgcagcta cagcttttag
     2881 tttttgttca tttcttgcta atagaacaac ctttgcccca agaagacaaa gctcttttgc
     2941 cgctgcctta cctatgcctt gtgtgctgcc acaaacaagg gcttttttat tcgttaaatc
     3001 cagattcata ttgcaaatgt acctgactta atttgataaa cattttttta ccataacttt
     3061 ggaaaacaaa aaaacacaaa atcatgtcaa atccaatagt agatcaggta agtaaggctg
     3121 ctaatattaa tcaggagcaa gctgccaagg caattgaagc tgtatctgca gcactaaaag
     3181 aaaaattacc ttatttgtta cataatcaaa tagacatcct tctcaccgga ggtaacttat
     3241 ctgacggtgt aaaacaaaaa ttcgaaactt taaaggatga tcttgaaagc tcggcaaaag
     3301 accttagcac aaaagcacaa gaatttaccg aggaagtatc taagaaactc aacaatctat
     3361 ttcaaaaata aattcaatct taaaaattat ggcagtcagc aaacctttta atcttcagca
     3421 gtgggttgaa gaaaaccgtc atctgttaaa acctcccgta ggcaacaaac aagtttatat
     3481 agacaataaa gattttattg ttatgatagt cggtggtcct aattcaagaa aggattacca
     3541 ttttgaagat gcagaagaat tattttatca gatagaagga gatattgtgg taaaaaccat
     3601 tcaggatgga aagccggttg atattcctat caagcaaggc gaaatgtttt tattacctcc
     3661 acgagtacca catagtccgc aaagaggtgc aaacactatt ggtttggtag tagag
//
LOCUS       DAOOOL010000194         3711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2866_length_3711_cov_127.546225, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000194 DAOOOL010000000
VERSION     DAOOOL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_3711_cov_127.546225"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..519
                     /locus_tag="PKU82_11290"
     CDS             286..519
                     /locus_tag="PKU82_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83490.1"
                     /translation="MPFYRITIWLKNKRKPVSGIRFIEQSNIDIVNIQMQKQARIHYN
                     DSLIIDVEVAMLSKNSKAVKQHQKEILSKSGKT"
     gene            925..1224
                     /locus_tag="PKU82_11295"
     CDS             925..1224
                     /locus_tag="PKU82_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83491.1"
                     /translation="MCICKNQDEKDFYFWLLFGLWKAKHFHQLLTGSVIPFIRIDEFK
                     KEVATQAEHVSKQRTAYAKTIDKIKLLEQKEKQLQQYLAVIQDVKRATIYRYLKR"
     gene            1437..1670
                     /locus_tag="PKU82_11300"
     CDS             1437..1670
                     /locus_tag="PKU82_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83492.1"
                     /translation="MHKQLRIISSRRLSVCPFSRSFFPPSVPISPLAFSRHARRLPVH
                     YHPLPGVAVYHMPCGGWLWCFPSGRRQLSALPF"
     gene            complement(1973..2776)
                     /locus_tag="PKU82_11305"
     CDS             complement(1973..2776)
                     /locus_tag="PKU82_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83493.1"
                     /translation="MARQTGLVKYNGTMGGVRHFKIKGLAGDFAGMAGGPSAEQINND
                     PAFIRTRENMNEFGGSAAAAKSVRVALSQIIKQFSDPRLTGRLTAIMKQINLEDQSEA
                     RGRRAIEISTQRQYLEGLEFDANLSLSGLFNAPYSLSNTVARDSATFTIPAFNPANLV
                     NAPAGATHFRLVNAIAVVSDWVYNDTTGKYEPTDIALNELADVKYSGYLDLNAVTAAT
                     TVTTTLPGSPTMTATVSVLNCVGIEFYQQVGSNYYLFASGNALRVDEVF"
     gene            2873..3364
                     /locus_tag="PKU82_11310"
     CDS             2873..3364
                     /locus_tag="PKU82_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOZ83494.1"
                     /translation="MQFDAIAPYLIETHYKITRVLDGDSFMVQNIFSKAEKEIRLYGL
                     DAPENKRNRKLIEDEKKARVAGEFLIQLGRKSTDFVLTIAPPGTNITLITEEKYFFDF
                     YKRQLAYIILPDGSCLNEILITEGYAKALDEYYCSNLSKYQQLNFLAKQSAKGLYAFT
                     GIF"
     gene            complement(3444..3517)
                     /locus_tag="PKU82_11315"
     tRNA            complement(3444..3517)
                     /locus_tag="PKU82_11315"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3481..3483),aa:Asp,seq:gtc)
BASE COUNT         1160 a          775 c          689 g         1087 t
ORIGIN      
        1 acaaacctgc acacaggctt tccgtgtcat ggcatcccga tagcctgcac acataatcct
       61 ttcacattcg ggattgctca tgccactaca agccacccta tacatttgct ccattgcgtt
      121 cagtcattgc atttgcttgt accatccgca cagtacaact ggtatgcttt atttttttct
      181 gcggcatatt accccggcca gaacacaaat acaaaatccc gttccataaa aaaatgattt
      241 ttcaatttca tcctacccat cctaccttta ctaataatat tagcaatgcc tttctatcgg
      301 ataactatat ggttaaagaa taaacgaaag cccgtatcgg gcatccgttt tatagaacaa
      361 tccaatattg atattgtcaa tatccaaatg caaaaacaag caaggattca ttacaatgat
      421 tctttgataa ttgatgttga agtagccatg ttatctaaaa acagtaaggc agttaaacaa
      481 catcaaaaag agatattgag taagtcagga aaaacataaa tccctttctc ttttttaaaa
      541 ttccgtttga catacaaata aaaaaatgca gcaaagttag ttggctggca cagccccatg
      601 ctttaaaaaa aacaaggaac tacggcataa acacagcccc accgcacaaa gccatcattt
      661 attccgttcc tccttgtttt tttagccacg ccacctgata aaattgcttt cttttttctt
      721 ttttttttcg gttttttttc ttttttcttt tgggttatgt ataggcggag cgtagcggag
      781 caataattaa atgctcatac tatgcacagt ccaaagtttc aaacattcag cgtaaaaaat
      841 caaagcacac aatttgaatt ttacattctt tgcaagggtt taaatagtgg gaagccgctt
      901 gataaacctt gcccaaattc ctatgtgtgt atctgcaaaa atcaggatga aaaagatttt
      961 tatttctggt tgctgttcgg tttatggaag gcaaaacatt tccaccaatt actaacaggg
     1021 tcggttatcc catttatccg cattgatgaa tttaaaaagg aagttgcgac acaggcagag
     1081 catgtaagca aacaacgtac agcctacgca aaaactattg ataaaataaa actgctggag
     1141 caaaaggaaa agcaattgca gcaatatctg gcagtcatac aagatgtgaa aagggcaacg
     1201 atataccgct acctgaagag ataaaaacaa aatcctgctt ttgaaatacg cagcttgtgc
     1261 agtaaaaaat ttttatacgc aaaagtagca ggggcgggca accgcacaga cccgacaaaa
     1321 aaaacaaaaa gactacggca taaacacagc cccaacacac agccccttct tttattccgt
     1381 ttccttttgg ttttttaatc cgcccttgct gagagaggcg tataaaattt ttacttatgc
     1441 acaagcagct tcgtatcatt tccagcaggc gtttatcagt ttgtcctttc agtaggtcat
     1501 tctttccgcc atcagttccc atttcgcctc ttgcgttcag tcgtcatgcc aggcgtttgc
     1561 cggttcatta ccatccgctt ccgggtgttg cagtttatca catgccttgc ggtggttggc
     1621 tttggtgttt tccatcaggt cgccgtcagt tatcagcatt acccttttag gggtagtgct
     1681 tttttctttt gatggtaagc taccagctta cagggttccg accctgtaaa gcctttattt
     1741 gaccttacac acaggatttg aaacactgct tgacagcaat ttatttccta catattcccg
     1801 atatgagccg tatatgattg caccattttg gaaaaaaaat gcaaagcaaa gcaacgaatt
     1861 acagtgaaat ttagcgatga ttgctgcatg attgcagagg atgcagcgag gggataaggc
     1921 ataaaaaaag ccccaccttt ccggtaaggg cattttttgc agtggaaaat atttagaaaa
     1981 cttcatcaac tcttaacgca ttacctgaag caaagaggta atagtttgaa cctacttgct
     2041 gataaaattc gatgcctacg caatttaaaa cgcttactgt tgcggtcatt gttggtgagc
     2101 ctggcaatgt agtggttacg gttgttgcag cagttacagc gtttaaatca agataacctg
     2161 aatacttaac gtctgcaagc tcattcaatg caatatcggt tggctcatac tttccggtag
     2221 tgtcattata aacccaatcg gaaacaactg caattgcatt taccaaacgg aagtgtgttg
     2281 cacctgccgg agcattaaca aggttggcag ggttgaaagc tggaatagta aatgttgcac
     2341 tatcacgggc aactgtattt gaaagtgaat agggagcatt gaacaaaccc gataaactca
     2401 aatttgcatc aaactccaaa ccttccaaat actggcgttg tgttgaaatt tcaatagccc
     2461 ttctacctct tgcttctgat tggtcttcca gatttatttg tttcataata gccgtaagac
     2521 ggccagttaa acgagggtca gaaaactgtt tgatgatttg ggaaagtgca acccttacgg
     2581 attttgcagc agcagcagag ccaccaaatt cattcatgtt ttcacgggtt ctaataaacg
     2641 ccgggtcgtt gtttatttgc tccgcagatg gcccaccggc catgcctgca aagtcaccag
     2701 caagcccttt gattttgaaa tgacgaacac cacccatagt gccgttgtat tttactaagc
     2761 ctgtttgccg tgccataact ataaaaattt agttgtttgt atttgaaaaa ttttacagta
     2821 caaaaataaa tctaacttcg ttataagtat ttgactatca ataagtaaaa caatgcaatt
     2881 tgatgctatc gcaccatatt taatagaaac tcattataaa atcaccaggg ttttagatgg
     2941 tgacagtttt atggtacaaa acatcttttc aaaagctgaa aaagaaattc gtttatacgg
     3001 tcttgatgca cctgagaata aaagaaatag aaagctgatt gaagatgaaa agaaagcgag
     3061 ggtagcagga gaatttttaa ttcagttggg cagaaaatca acagactttg ttttaacgat
     3121 tgcaccaccc ggcacaaaca ttacattaat aacagaagag aagtattttt ttgattttta
     3181 caagaggcaa ttagcataca tcattttacc tgatggaagt tgcttgaatg aaatactaat
     3241 aacagaaggc tatgcaaaag cattggatga atactattgt tccaatcttt caaaatatca
     3301 gcagttgaat tttttagcga agcaatctgc aaagggtttg tatgctttta caggcatatt
     3361 ttgaattgtt taccctatgt ttaccccgat atgaaaaaag gcttgcaaat cattgattta
     3421 caagccttta gacattttaa aagcggaccg gacgggactc gaacccgcga cctccgccgt
     3481 gacagggcgg cattctaacc aactgaacta ccgatccaat atttttatta aagaactttt
     3541 ctactcgaac ccgtccccaa cacgttgggg atgacagggc ggcattctaa ccaactgaac
     3601 taccgatcca atatttttat taaagaactt ttctactcga acccgtcccc aacacgttgg
     3661 ggatgacagg gcggcattct aaccaactga actaccgatc caatattttt a
//
LOCUS       DAOOOL010000195         3705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2878_length_3705_cov_72.463836, whole genome shotgun sequence.
ACCESSION   DAOOOL010000195 DAOOOL010000000
VERSION     DAOOOL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_3705_cov_72.463836"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..2572)
                     /locus_tag="PKU82_11320"
     CDS             complement(311..2572)
                     /locus_tag="PKU82_11320"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOZ83495.1"
                     /translation="MPVNKEALDYHASGRPGKIEVIPSKPYSSQRDLSLAYSPGVAEP
                     CLEIEKDIDNVYKYTAKGNLVAVISNGTAVLGLGNIGPEASKPVMEGKGLLFKIFADI
                     DVFDIEINQTNVDDFVKTVKAISPTFGGINLEDIKAPECFEIESRLKNELNIPVMHDD
                     QHGTAIISGAALINALDIVNKKIDLVKIVVNGAGASAISCTKLFVSLGAQKKNITMLD
                     STGVIRKDREGLDANKREFATDRNINTLEEAMRGADVFIGLSKGNILSADMLKSMADK
                     PVVFAMANPNPEITYPDAKSAREDVIMATGRSDFPNQVNNVIGFPFIFRGALDVRATK
                     INEEMKLAAVHAIAQLARQPVPEMVNLAYNTKNLQYGPEYIIPKPLDPRLIYTVAPAV
                     ARAAMETGVAQVSITNWEHYVEDLKKRLNLDNKLIRAIVTRAKQNPQRIVFAEADNYK
                     ILKAAQQVKEEGIARPILLGNRERITQIVTENHIDITDIPIVDPRVDTEHLNTFGELF
                     FEKAKRRGFSVYEAKKVMRERNYYGAMMVETGQADALISGLTRNYADTIRPALRIIGV
                     QEHGTRVAGMYIMMTKKGPLFFADTTINVNPTSEDLVEITLLTAKAVQQFNIKPRIAL
                     LSFSNFGTSNDPSAIRIRKAVEILHDKFPGMIVDGEMQANFALNMQMLHDSYPFCELI
                     NGGVNTLIFPNLDAGNIAYKLLQSMEAAEAIGPVLLGMKKPVHILQLGSSVRDIVNMV
                     TIAVIDAQSRKQQ"
     gene            complement(2680..3183)
                     /gene="ribH"
                     /locus_tag="PKU82_11325"
     CDS             complement(2680..3183)
                     /gene="ribH"
                     /locus_tag="PKU82_11325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOZ83496.1"
                     /translation="MALYQENNSLYNPAEYPDASSVKVGIVTASWNNKITSKLFDGAA
                     DVLKKSSVKENNLITIQVPGSYELTAGAQWLFQQHAPDVVIALGCVIQGETRHFDFIC
                     NAVANGLNHVSLKFGKPVIFGVLTTDNEHQALERCGGTHGHKGCEAAFTALSMALLKT
                     PVAKIGF"
     gene            complement(3190..>3705)
                     /locus_tag="PKU82_11330"
     CDS             complement(3190..>3705)
                     /locus_tag="PKU82_11330"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ83497.1"
                     /translation="QQANAEKQMFMAEYYFKNDSLDKAIKGDGNFPGFEEIISSYGSS
                     KSANLAHYYLGVSLLRKGQYDDAITALSKYDAEDDITGAIALGCIGDAYMEKGNKEEA
                     MNFYKKASDYDQNQFTAPIYLMKQAMVLELNNDYKSAYDIYNRIKRDFPNSTEARQIA
                     TYMARAEAHMN"
BASE COUNT         1006 a          731 c          805 g         1163 t
ORIGIN      
        1 tataattatg aatgcagagt tcagaattca gtattatcaa ttagccacgg ctgatatggg
       61 tgagaaatgt aaaatgtaaa ataataaatc taaaatgtaa aagtgaaaag tgatgagagc
      121 aactaataca cagcaagaaa atgttttaca cataaacaat tagccctgat gatcccgata
      181 gcgatcggga ggaggttttg tgcgtgagcg gtgccatgca ccgaccccga taattatcgg
      241 agaaactatt gtaaaagtta ctgcagtttg tgttctaaaa aattatctac tcaaacaaga
      301 cgtttaacca ctactgctgt tttctgcttt gcgcatcaat aacagcaatt gttaccatgt
      361 ttactatgtc gcggacgctg ctgccgagtt gcagaatgtg taccggcttt ttcatgccaa
      421 gaagtacagg accaatagcc tctgctgcct ccatagattg cagaagtttg taggcaatgt
      481 ttccggcatc gaggtttgga aagatgagtg tgttgacacc accgttaatg agctcacaaa
      541 acggatagct gtcatgcagc atctgcatgt tgagtgcaaa gtttgcctgc atttcgccat
      601 ctactatcat acccggaaat ttgtcgtgta atatttctac agcttttctg atgcgaatag
      661 ctgaaggatc gtttgaagtg ccgaagttgg agaatgacaa gagtgcaatg cgcggcttta
      721 tgttgaactg ctgcacggct ttggctgtga gcaaggttat ttccaccaga tcttctgatg
      781 taggatttac attgatggtg gtgtcggcaa aaaacaaagg gccttttttt gtcatcataa
      841 tgtacatacc tgcaacacga gttccatgtt cctgcacacc gatgattcgt aatgccggac
      901 ggatggtgtc ggcatagtta cgtgtgaggc ctgagatgag tgcatcggcc tgtccggttt
      961 ctaccatcat ggcaccgtag tagtttcttt cgcgcattac ttttttagct tcgtaaacgg
     1021 agaagcctct gcgttttgct ttttcgaaaa agagttcgcc aaaggtgttg aggtgttcgg
     1081 tgtcaacacg agggtcaacg atgggaatgt ctgttatatc aatgtggttt tctgtgacga
     1141 tttgtgttat tcgctcgcgg ttgccgagca aaattggtcg tgcaattcct tcttctttga
     1201 cctgttgtgc agctttaaga attttgtagt tgtcggcttc tgcaaagact atgcgttgcg
     1261 gattttgttt ggctcgtgtt acaattgcac gaatgagttt attatcgagg ttgagacgtt
     1321 ttttgaggtc ttctacatag tgttcccagt tggtgatgct gacttgtgcc acacctgttt
     1381 ccatagcagc acgtgcaacg gcaggtgcaa cggtataaat caatcgtggg tcaagtggtt
     1441 tggggataat gtattcgggg ccgtactgca gattttttgt gttgtaagca aggtttacca
     1501 tttcgggaac tggctgtcgt gccaactgcg caattgcatg caccgctgcc agtttcatct
     1561 cttcgttgat ttttgttgca cgcacatcta aggctcctct gaaaataaat ggaaaaccaa
     1621 ttacgttatt tacctgattg ggaaagtcgc tgcggcctgt tgccatgatt acatcttcgc
     1681 gtgcactctt ggcatcggga taagtaattt ccggattggg attggccata gcaaaaacaa
     1741 caggtttgtc ggccatagat ttgagcatgt cggcactgag gatatttcct ttgctgagac
     1801 caataaatac atcggcaccg cgcatggctt cttcaagcgt gtttatgttt ctatcagttg
     1861 caaattctct tttgttggca tctaaccctt cgcggtcttt tctgataaca ccggtactgt
     1921 cgagcatggt gatgtttttt ttctgtgcac caagcgaaac aaaaagtttg gtacatgaaa
     1981 ttgcagaagc acctgcacca ttgacaacaa tttttacgag atcaattttt ttgtttacaa
     2041 tatctaatgc attgatgagt gctgcaccgc tgatgatggc agtgccatgc tgatcgtcat
     2101 gcattacagg aatgttcaac tcattcttta acctactttc aatttcaaaa cattctggtg
     2161 ctttaatgtc ttcgaggttt atgccaccga aagttggtga aatggctttt actgttttta
     2221 caaaatcgtc aacgtttgtt tgattgattt cgatatcgaa tacatcaatg tctgcaaata
     2281 ttttaaacag cagtccctta ccttccatga caggttttga tgcttcgggg ccaatgttgc
     2341 caagaccaag tacggcagtg ccgttagaaa ttacggcaac aagatttcct ttggcagtgt
     2401 atttataaac gttgtcaatg tctttttcaa tttcaagaca aggctctgca acaccgggcg
     2461 aataggctaa tgataaatcg cgctgtgagc tataaggctt actgggtatc acttcaattt
     2521 tgcccggacg acctgaggca tgataatcga gtgcttcttt attaactggc ataattattt
     2581 tttttacaaa gtgctaaagt aaagaataca acatcacgtc attgcagaaa ataaacagat
     2641 gagatactga atttaatcag cttaaaataa atttaaaatt taaaaaccga ttttggcaac
     2701 aggagttttg agaagtgcca tgctcaatgc agtgaatgcg gcttcacacc ctttatgtcc
     2761 gtgagtgcct ccacaacgtt caagtgcttg atgttcgttg tcggtagtga gtacgccaaa
     2821 aattacaggc ttgccaaatt ttaacgaaac atgattgagt ccattggcaa cagcattgca
     2881 gataaaatca aaatgacgtg tctcgccctg aatgacacaa cccagtgcaa taacaacatc
     2941 aggagcatgt tgttgaaaaa gccattgtgc acctgcagta agttcatagc tacccggcac
     3001 ctgaatagtg atgaggttat tttcttttac agaagatttt ttaaggacat cagcggctcc
     3061 gtcaaaaagt tttgaggtga ttttattatt ccaacttgct gtaacaattc caacttttac
     3121 cgaagatgca tcaggatatt cagcagggtt atagagagaa ttattttcct gatataatgc
     3181 catgaaaaat tagttcatgt gtgcttctgc acgcgccatg taggttgcaa tctgacgtgc
     3241 ttcagtagag tttgggaagt ctctttttat tctgttataa atatcgtagg cagatttata
     3301 gtcgttgttc aactctaaaa ccattgcctg cttcatcaga taaataggcg ctgtgaattg
     3361 attctgatca taatctgaag cctttttata aaagttcata gcctcttctt tattgccttt
     3421 ttccatgtac gcatcaccaa tacatcctaa agcaatagct cctgtaatat cgtcttctgc
     3481 atcgtattta gataaagcag taatagcatc atcatactgt cctttgcgca acaaacttac
     3541 accaaggtag tagtgtgcca gattagctga ctttgatgaa ccatagctgg aaataatttc
     3601 ttcaaagccg ggaaaatttc catcaccttt gatggcctta tccaaagaat cgtttttgaa
     3661 ataatattca gccatgaaca tttgtttctc ggcattagcc tgctg
//
LOCUS       DAOOOL010000196         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2919_length_3663_cov_53.011918, whole genome shotgun sequence.
ACCESSION   DAOOOL010000196 DAOOOL010000000
VERSION     DAOOOL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_3663_cov_53.011918"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKU82_11335"
     CDS             <1..221
                     /locus_tag="PKU82_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83498.1"
                     /translation="SGSSIDGTAIGLSQGTEAGSGNNDTSIDWDLTNEKNVPISSGVY
                     LIHVKAPGLGERTLKWFGVMRPISLDSY"
     gene            251..1303
                     /locus_tag="PKU82_11340"
     CDS             251..1303
                     /locus_tag="PKU82_11340"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOZ83499.1"
                     /translation="MTYKFKISAIAITSLLLSASAFAGNEDRVGQSGANELIINPWVR
                     SAGWMNCNTSSVRGLEAQYLNVAGTAFTKKTEIGFSHTNWLKGTGISINSFGITQHVG
                     ESGALTLGIMSMDFGNIEITTPESPEGGIGTYKPQFLNVGLSYAKAFSNSIYGGITIK
                     IISESVADVRASGIAFDAGIQYVTGFNKEKDNLRFGIALKNVGAPLNFKGDGLASKGF
                     TSDQTSLTISQRAERFELPSLVNIGAAYDFKFLEMHRITLSSTFTSNSFTRDQTAVGL
                     EYGFKKFFALRAAYSFERKGTSEDESLNVYSGFGAGITVEAPIGKSGKTIGLDYSYRP
                     TYTFDGTHAFGVNFKL"
     gene            1376..1849
                     /gene="greA"
                     /locus_tag="PKU82_11345"
     CDS             1376..1849
                     /gene="greA"
                     /locus_tag="PKU82_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOZ83500.1"
                     /translation="MMAITYLTEEGLKKLQDELTNLKTVQRPLISKQIAEARDKGDLS
                     ENAEYDAAKDAQGLLELKISKMEELLSSARIVDESKIDTSKVSILSKVKIKNLTNKQE
                     VTYTLVAENEADLKAGKISVTSPIGKGLLGKKKGEVAEITVPSGKLNFEIVSIDR"
     gene            1852..2247
                     /locus_tag="PKU82_11350"
     CDS             1852..2247
                     /locus_tag="PKU82_11350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOZ83501.1"
                     /translation="MATIFTKIINGEIPAHKIEENDNYLAFLDINPIATGHVLVIPKR
                     EVDYIFDLKDEELAGLMAFSKQISKALIQAVPCKRIGVTVIGLEVPHTHVHLVPINNV
                     DDLNFGKPKLKLTNEQLELIRLKIKSFLK"
     gene            2313..3104
                     /locus_tag="PKU82_11355"
     CDS             2313..3104
                     /locus_tag="PKU82_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83502.1"
                     /translation="MNKIKSLSLFSAIALAALSGCKKDDLTSDFKTSDPIQNGTAKIR
                     FIHVAPELGGVSVKYKDNSVFGDSLRYSQNSGYTEVALGSVDNGFNLDMTPIEDSTGI
                     FTPNETGVIATLAQSNYILAKDQRYTCFIIDTVKAEPVLNDLSYDGFTKIITENYPAT
                     GTDSVWVRLFNASYSSAIVDIEFTNNTSGAVTTISNVSFMQVPVYAGAGSGDYNVAVK
                     KFGITLASANNVNLQSGKAYTFYFRGVIGASGDTEPRLDYITNGE"
BASE COUNT         1220 a          574 c          714 g         1155 t
ORIGIN      
        1 attcaggttc atcaatagat ggcacagcca tagggttgag tcagggcaca gaggcaggca
       61 gtggcaacaa cgacacatca attgactggg acttgacgaa tgagaagaat gtaccaatca
      121 gcagtggtgt atatctgata catgtaaaag cacctggctt gggtgagcga acccttaagt
      181 ggtttggtgt aatgcgtccg attagtttag atagttatta ataaacctcc taaccaaaat
      241 atattgaaat atgacatata aattcaaaat atcagctata gcaatcacat cgttattgct
      301 atctgcatca gcttttgcag gtaatgaaga tcgtgttgga caatcgggtg ccaatgagtt
      361 aattattaac ccatgggtgc gtagtgcagg ttggatgaac tgtaacacat cttcagttcg
      421 cggattggaa gctcaatacc tgaacgtggc cggtactgct tttacaaaaa agacagaaat
      481 tggtttttca cacaccaatt ggttaaaagg aacaggtata agcattaact catttggtat
      541 tacacaacat gtgggtgaat ccggggcatt aactttaggt atcatgtcta tggattttgg
      601 taatattgag attaccactc ctgaatcgcc tgaaggtggc ataggaacat ataaacctca
      661 atttctaaat gttggtttgt cttatgcaaa agcgttttcg aatagtattt atggtggtat
      721 aactataaaa attatttcag aaagtgttgc cgatgtacgt gcttccggaa ttgcttttga
      781 tgcaggtatt caatatgtaa caggatttaa caaagaaaag gataaccttc gttttggtat
      841 tgcattaaag aatgttggcg cacctttgaa ttttaaaggt gatggattgg catcaaaggg
      901 attcacttct gatcagacat cattaactat ttcacagcgt gctgaacgtt ttgaacttcc
      961 atcattagta aatattggtg ctgcttatga ctttaaattt ttagagatgc atcgtataac
     1021 actgtcttca acatttacat ctaattcttt cacacgcgat cagacagcag taggtttgga
     1081 atatggattt aaaaagttct ttgctttacg tgctgcttac tcattcgaaa gaaaaggaac
     1141 aagtgaagat gaatcactga atgtttacag tggctttggt gccggtataa cagttgaagc
     1201 tcctataggt aaaagcggaa agacaattgg attggattat tcttatcgcc caacttatac
     1261 gtttgatggt actcatgcct ttggtgtgaa tttcaaattg taaaaactaa ttaattttta
     1321 aattatttta aaggaggtct taacaggcct tctttcattt ttttaatacc cacatatcat
     1381 ggctattact tatttaacag aagaaggact aaaaaaactt caggatgaat tgaccaatct
     1441 gaagaccgtt caacgacctc taatttcaaa gcagattgca gaagcaagag ataaaggtga
     1501 cttgtctgaa aatgcagagt atgatgctgc aaaagatgca caaggattgc tcgaattaaa
     1561 gatcagcaaa atggaagagt tgttgagtag tgcacgtatt gttgatgaat ctaaaattga
     1621 tacatcaaaa gtgtcaatac tttctaaagt gaaaataaaa aacttgacta ataagcaaga
     1681 ggtaacttac actttggttg ctgaaaatga agcagattta aaggcaggaa agatatcagt
     1741 aacatctccg ataggaaagg gtcttttagg taaaaagaaa ggtgaagttg ctgagattac
     1801 agttccttcc ggaaaactta attttgaaat tgtttcaata gacagataat catggcaacc
     1861 attttcacaa aaattattaa tggcgaaatt cctgcccata aaatagagga aaacgataat
     1921 tatttagctt ttttagatat taaccctatt gcaacagggc atgtgctggt aattccaaag
     1981 cgtgaagttg attatatttt tgatttgaaa gatgaggagt tggctggact aatggcattt
     2041 tccaagcaaa tcagtaaggc tttaatacaa gctgtgccat gtaaaagaat tggtgttaca
     2101 gttattggac ttgaagtgcc gcatacacat gtacatctgg tacctattaa caatgttgat
     2161 gacctgaact ttggtaagcc caaattaaag ctgacaaatg agcaattgga attaatccgg
     2221 ctgaaaatta aatcgttttt aaaataaatt ttcagtttta atttaaatta cgttatttcg
     2281 tatctgcatt ttaacctgta tatttttaaa ttatgaataa aataaaaagc ctttcattgt
     2341 tttcagcaat tgccttggca gcactttcag gatgtaagaa agatgatctt acttcagatt
     2401 tcaaaacctc tgacccgatt cagaatggta cggcaaaaat tcgtttcatt cacgtagcac
     2461 cggaattagg tggtgtttct gttaagtata aagacaatag tgtgtttggt gactcattac
     2521 gctattcgca aaactcaggt tatactgaag ttgccttagg ctctgttgat aatggattta
     2581 atttagatat gactcctatt gaggattcaa caggtatttt cacacctaat gaaacaggtg
     2641 taattgcaac acttgcacaa agcaactata tcttggcaaa agatcagcga tacacatgtt
     2701 ttattattga cactgtaaaa gctgagccgg ttttaaacga tctctcttac gatgggttta
     2761 caaaaatcat cactgaaaat taccctgcta caggaacaga ttcagtttgg gtacgtttgt
     2821 ttaatgcatc atattcttca gccattgtag atatagaatt taccaataat acttctggcg
     2881 cagtaactac catttcaaat gtcagcttta tgcaggtgcc tgtttatgca ggtgccggta
     2941 gcggtgacta taatgttgca gtaaagaaat tcggtataac attggcttct gctaataatg
     3001 ttaacctaca gtctggcaaa gcttatactt tttatttccg tggtgtaatt ggtgcatccg
     3061 gtgataccga gccacgtctt gattatataa ctaacggaga gtaagtttaa tttggaatat
     3121 ttgaatggct gctgtgtttt catagcagcc attttttttg ccattgattc tgtttttcaa
     3181 atataatgtt gaagaagttt aaaattaatt gccggaaaaa gtttgctttt cactatttgt
     3241 aattattatt taatttcata tttttaatac ctcaaataaa cgaagataaa atagtttgaa
     3301 aacgcttgaa attataattg caaaaatcac ccaatacaat aattggaaaa aattaaaata
     3361 ataattttta tcaaaataga aaacgaaatt ggtaatcatg ttgtcttaga gtataaaggc
     3421 atatttaaca agtgttattt tgaattataa aagtgattat aaaccatatc taaaacaaag
     3481 cacaaatgat taataagagt tgtttaacgg tatttctggc agcagcaatt acagtaggtt
     3541 cagcatcagc caaggagaat acaggtcaga agccggtaca taaaggcaat gttgtaaaaa
     3601 acgcgtcatt gagaattaca gccccctgta atgagcccac ggcacaaaca gatttgcaga
     3661 tta
//
LOCUS       DAOOOL010000197         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2953_length_3624_cov_92.205380, whole genome shotgun sequence.
ACCESSION   DAOOOL010000197 DAOOOL010000000
VERSION     DAOOOL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2953_length_3624_cov_92.205380"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKU82_11360"
     CDS             <1..192
                     /locus_tag="PKU82_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ83503.1"
                     /translation="NSQATWRTKHADFYLGCENITDYRQKHAIIAADEPFGQNFDASM
                     IWGPLMGRTFYVGFRYTIK"
     gene            202..546
                     /locus_tag="PKU82_11365"
     CDS             202..546
                     /locus_tag="PKU82_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOZ83504.1"
                     /translation="MKKLIAGAVMLLFFSFSLMAQQNKKAEINIATSAECTMCKKALE
                     TALLHQHGVKFAKLDLNTRVVAVKYNGEKVTPETLRQVISNAGYDADSVKANPEAFEK
                     LPDCCKNGALHH"
     gene            complement(569..1255)
                     /locus_tag="PKU82_11370"
     CDS             complement(569..1255)
                     /locus_tag="PKU82_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOZ83505.1"
                     /translation="MNNRSYKILIVDDEPDILEFIQYNLRKEGYFVYTAKNGEEAVAL
                     AKQEVPHLIVMDIMMPVMDGVEACRIMRTIPALNKTIITFLTARSEDYSQIAGFDVGA
                     DDYIQKPIRLPVLMSRIKALLRRASELPVADASVIEVGGLKIDRMHYLIYQNGQTFEL
                     PRKEFELISLLASKPGKVFSREDILSRVWGNDIVVGNRTIDVHIRKIREKLGDQVIKT
                     IKGIGYKIEP"
     gene            1433..2878
                     /locus_tag="PKU82_11375"
     CDS             1433..2878
                     /locus_tag="PKU82_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83506.1"
                     /translation="MKKLLLLLLIGLTVKCAAQDYAGVRVGDTAHYYHQTNYYFPLGK
                     LKAIAIDSVAVNANETELFNTFLIHGQDPLCIKYDRENWTGKKITRTINGIEKYITGN
                     NDTIVVDPITQVGSSWVFCNLANNTHFDATVLSVQPESFLNISDSVISFSLQLKDNSS
                     QNIAHPFNGKTLKLSKQYGWIHTINFLDFPTDTALLTITGTTQSNLGYQNLIADSIFN
                     FNIGDTFQYHDYGQSAINQWDEYFLSLTILTKNISTNGDTLLYTADRQTYNRNFSAPN
                     LLVNYTHDTTTLIYPLSNYSYLNKLEEKIDSANFAIKGVFVDSRQTCGNNIWTKAVFT
                     CLYNLSDSCLGILIDCTNTFNYANGLGMVSFDASTFYPVYTELIFYSKNGVSCGTPIN
                     FNQLLSVNEINKESFTLLNNPTNGILRIQTTIPESIITIREPSGKEIWHKTIKNTGLV
                     ELPLNVANGIYLLTVENQNNRTTKKLIVNNY"
     gene            complement(2980..3459)
                     /locus_tag="PKU82_11380"
     CDS             complement(2980..3459)
                     /locus_tag="PKU82_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83507.1"
                     /translation="MTNYKNLIYALLCLSFSIIVGGSLFEHIAVWPNAFAAPPKSLTM
                     FQGEYAFNPEPFWKSIHPVTLILFIITLIISWNTTRKKNVLIAFMGYVVILIATFIYY
                     LPELKDLIATPFADLINQDLVQRGARWQTLSLFRTALAIILALILYFGLSKSDNKLS"
BASE COUNT         1250 a          646 c          552 g         1176 t
ORIGIN      
        1 aattcgcaag caacctggag aaccaaacat gcagattttt atttaggttg cgagaatatt
       61 acagactata gacaaaagca tgctattatt gccgctgatg aaccttttgg tcagaatttt
      121 gatgcttcaa tgatatgggg gccattaatg ggtcgcacat tttatgtagg atttcgttat
      181 acaattaaat aaacaataaa tatgaaaaag ttaattgcag gtgccgttat gttattgttc
      241 ttcagtttct cactgatggc acaacaaaat aaaaaagctg aaataaacat tgctacttcg
      301 gcagaatgta ccatgtgcaa gaaagcctta gagactgcct tactgcatca acatggtgta
      361 aaattcgcaa agctcgacct taatacaaga gtggttgctg ttaaatacaa tggcgaaaaa
      421 gttacaccgg aaacacttcg ccaggtaatc agtaatgcag gttatgatgc agatagtgta
      481 aaagccaatc ctgaggcatt tgaaaaactg cctgactgtt gcaagaatgg cgcattgcat
      541 cattaggcat tagtgaaaag agaatctttc agggttcaat tttataacca attcctttaa
      601 tggttttaat tacctgatca cctaactttt ctcttatttt tctaatgtga acatcaatag
      661 ttctattgcc tactacaata tcattacccc acacccggct gagtatatct tctctcgaaa
      721 aaactttgcc gggcttggat gccagtaaag aaattaattc aaactccttt cgaggtaatt
      781 caaaagtttg gccgttttga taaataagat aatgcatgcg atcaattttt aatccgccta
      841 cctcaattac tgatgcgtct gccacaggca actcactcgc tctgcgcagc aacgctttaa
      901 tgcgactcat caacacaggt aaccgaatag gtttctgtat gtaatcatca gcacctacat
      961 caaaccccgc tatctgagaa taatcttcac ttcgcgctgt taaaaatgtt ataatagttt
     1021 tattaagagc aggtattgtg cgcattatac gacaggcctc aacaccatcc atcacaggca
     1081 tcataatgtc cattacaatc aaatgaggca cttcctgctt agccaaagca actgcttctt
     1141 ccccattctt agctgtgtaa acaaaataac cttccttcct tagattatat tgaataaact
     1201 caaggatatc cggttcatca tcaacaatca atattttata agaccgatta ttcatttttc
     1261 tataactaat gcaaagaaag ttaactgcat tgaattttga atctataaat taaattttct
     1321 caaacttttg cgttgtttta gaatgacatt ctttaaattc ctgttccatt caaaaatgac
     1381 caaccaattt gcttgtttga attattctgt atatttacat aaatatattc tcatgaaaaa
     1441 attactactg ctccttttga ttggattaac agttaaatgt gcagcacaag attatgcagg
     1501 tgttcgtgtc ggtgatactg cacattatta tcaccaaact aattactatt tcccactagg
     1561 taaattaaag gctattgcga ttgacagcgt tgctgttaat gcaaatgaaa cagagctttt
     1621 caacactttt cttattcatg gtcaagatcc attgtgtata aaatatgatc gagaaaactg
     1681 gaccggcaaa aaaattacaa gaaccattaa cggcattgaa aaatatataa ccggtaataa
     1741 tgacacaatt gttgtagacc ctataacaca agttggatca tcatgggttt tttgtaattt
     1801 agcaaacaac acgcattttg atgcaacggt tctttctgta caacctgaat cctttcttaa
     1861 tatcagcgat agtgtaataa gtttctcatt acaattaaaa gataattcca gtcaaaatat
     1921 tgcacatcct ttcaacggaa aaacgttaaa gctgagtaaa caatatggtt ggatacatac
     1981 aataaacttc cttgattttc caacagatac agccttattg acaataacgg ggactacaca
     2041 gtcaaattta ggttatcaaa atctaattgc tgacagcatc ttcaatttta atattggtga
     2101 tacttttcaa tatcatgatt atggacaatc tgcaattaat caatgggatg aatattttct
     2161 ttctttgacc attttgacta aaaatatttc tacaaatggt gatacactgc tatacacagc
     2221 agacagacaa acttacaatc gcaatttttc tgctccaaat ctattggtta attacacaca
     2281 tgacactaca actttaatat atccactgtc aaattattca tatttaaata aattagaaga
     2341 aaaaatcgac tcagctaatt ttgctattaa gggagttttt gtggattcaa gacagacttg
     2401 cggcaataat atctggacaa aagctgtttt tacttgtctt tataatttaa gtgactcttg
     2461 tttgggtatt ctcatagatt gtactaatac tttcaattac gcaaatggtt taggtatggt
     2521 tagttttgat gcttctactt tttacccggt ctatacggag ttaatatttt actcaaaaaa
     2581 tggtgtttca tgtggcactc caataaattt taaccaatta ctttctgtta atgaaattaa
     2641 caaggaaagt tttacattat taaacaatcc aacaaatgga attttacgca tacaaacaac
     2701 cataccggaa tctataatta caattagaga accttcagga aaagagattt ggcataaaac
     2761 tataaaaaac acagggttag ttgaattacc tttaaatgtt gcaaacggaa tttacttact
     2821 caccgttgag aatcaaaata acagaacaac taaaaagtta atcgttaaca actattaaat
     2881 ttcgttttat catcaagtgt atgtagctac ttttttctat ttaaaaattg ttctttaatt
     2941 tataacctct ataagctatt gaatcgaaat tctataactt taacttaatt tattatcaga
     3001 ttttgataat ccaaaataca atatcaaagc taaaataatt gctagtgcag tccgaaataa
     3061 acttaatgtc tgccagcgag cacctcgttg tactaaatcc tgatttatta aatcagcaaa
     3121 tggtgtggct attaaatcct taagttccgg aagatagtaa ataaatgttg ctatcagaat
     3181 caccacatat cccataaaag caatcagcac atttttcttt cgcgtggtat tccatgatat
     3241 aattaaggtg ataatgaata aaatcaatgt caccggatga atactcttcc agaaaggttc
     3301 cggattaaaa gcatattcac cctgaaacat tgttaatgat tttggaggcg cagcaaatgc
     3361 atttggccat acagcaatgt gctcaaataa tgatcctcca acaattatag aaaaacttaa
     3421 gcacagtagc gcataaatca aatttttata gtttgtcatt ttgttaggat taaatatgcg
     3481 gaacaaattt gaaattagaa aaaaagacta aattgtattt aatggacatt ctcaaaaata
     3541 tgaccaacga tattgctttt tctattcaca tattcatgtg gtgtaatgta actcaaactt
     3601 ttgaacgttt ttataaaatg agat
//
LOCUS       DAOOOL010000198         3596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_2988_length_3596_cov_43.188365, whole genome shotgun sequence.
ACCESSION   DAOOOL010000198 DAOOOL010000000
VERSION     DAOOOL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_3596_cov_43.188365"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKU82_11385"
     CDS             complement(<1..1190)
                     /locus_tag="PKU82_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOZ83508.1"
                     /translation="MSEVVLKVALVGNPNSGKSTLFNVLTGLNQKVANFPGVTVEKHV
                     GFFSLRNAFEGVDYKVELTDLPGIYSLYPKTLDEQIPYKVLTDPQDPSFPNRIIVIAD
                     ASNLKRSLFLCSQIIDLKIPVVLVLNMMDLAVHKGLLPDVKKLEQALGIKVIPAIVRQ
                     KTGIEEIKKAVLHTIPLPESDIMPATTLAPELIQEIKQSFPVKSDYAAYLLAGNYDKV
                     DISFVGNEGKQKLDMLLQKHQFSQKTMQATETLERYKTISVLLKSCVSEPASVRKSLT
                     RTVDNLVTHRIWGYGIFLAILFVIFQFIFNVAQFPMDFIDGTFADFSQKVSELLPKGV
                     LNDLIVNGILAGLGGIIIFVPQIALLFLFITILEDTGYMARVSFIMDKLLRPFGLNGR
                     SVIP"
     gene            complement(1208..1435)
                     /locus_tag="PKU82_11390"
     CDS             complement(1208..1435)
                     /locus_tag="PKU82_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOZ83509.1"
                     /translation="MEKKLSELKPGEAGVIQNFSDEEIKLKLMEMGCIPGETVLMERV
                     APLGDPMAIKVAGYQLSLRKNEAETVWVSTL"
     gene            1624..2469
                     /gene="mreC"
                     /locus_tag="PKU82_11395"
     CDS             1624..2469
                     /gene="mreC"
                     /locus_tag="PKU82_11395"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOZ83510.1"
                     /translation="MRNLFLFLSRNYIFILFILLEAVCFFLIAQNNSYQNAATLNSTN
                     KVVASVYSTISSVTDYLDLAENNRRLAEENAHLRTLLPASYFDTTVVRNHKVDTIHNL
                     EYSYIEAKIINNSTNRRNNYMTLNKGSDAGIAPEMGVITGHGIIGIIKNVSAHYSTVT
                     SFLHTQSKVSVRFKNNNYNGSMVWDSDNSPTEATVIDIPRHVPFKVGDTLVTTTFSPY
                     YPEGIMVGTVKSFDIDAGSNSYRIILKLSTSFNDVSYVYVVNNLLKNERKQLEEKHKD
                     ATRGN"
     gene            2450..2968
                     /gene="mreD"
                     /locus_tag="PKU82_11400"
     CDS             2450..2968
                     /gene="mreD"
                     /locus_tag="PKU82_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOZ83511.1"
                     /translation="MLPEVIKSVLRFFLLILLQVILLNKIRFAGYINPFVYPLFLLLL
                     PVRIPKTLLLILAFITGISVDIFSDTLGMHAAASVFLGFMRPYIIKLLSPRDGYDSDA
                     VISLKQMGILWMVYYLFISIFLHHLFLFYTEVFRFNDFGATFLRVCLSTITSVVIIFI
                     ILLLFDKTKVQR"
     gene            2980..>3596
                     /locus_tag="PKU82_11405"
     CDS             2980..>3596
                     /locus_tag="PKU82_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOZ83512.1"
                     /translation="MKTSHSTQRLVLLITIALVAITYIGRLFYLQVIEDKYKLSADEN
                     VLRRIIDYPSRGLVYDRNGKLLVFNQTVYDLMITPREVKEMDTAEFCSIIKISIEDFR
                     KKFQKAKNYSPYRSSIFEKQISAETYGTLQEKLFKYKGFSVQPRTIRKYPYNTAAHLL
                     GYLGEVDENVTAKNNYYQMGDYIGISGIEQSYEKELRGRRGTRYM"
BASE COUNT         1128 a          702 c          625 g         1141 t
ORIGIN      
        1 aatggaatta cagaccttcc gtttaatcca aatggcctga gcaacttatc cataataaag
       61 ctaacacgtg ccatatagcc tgtgtcttca aggatggtta taaataaaaa caataaggca
      121 atctgcggaa caaatatgat gatgccacca agacctgcaa gaataccatt tacaatcaaa
      181 tcattcaaca cacctttagg aagcaattcg cttacttttt gagaaaaatc agcaaaggtt
      241 ccgtcaataa aatccatcgg aaactgtgcc acattaaata taaactgaaa gattacaaac
      301 agaatagcca gaaaaattcc atagccccat attctgtgtg taaccagatt atcaacggta
      361 cgggtcaggg atttacgcac tgatgcaggc tcgctgacac aacttttaag aagaacagaa
      421 atagttttat aacgctccaa tgtttcagta gcttgcatag ttttttggct gaactgatgc
      481 ttctgtaaaa gcatgtccaa tttttgttta ccttcattac caacaaaact tatgtccacc
      541 ttatcataat tacctgcaag caggtaggct gcataatcac ttttaaccgg aaaactttgt
      601 ttgatttcct gaataagttc aggagccagg gttgttgcag gcataatatc actttcgggc
      661 aaaggaatag tatgcagaac agccttcttg atttcttcaa tacctgtttt ctgacgtaca
      721 attgccggaa taactttgat gcctaatgcc tgttccaatt tttttacatc gggcaacaaa
      781 cctttatgaa cagctaagtc catcatgttc aacaccagca caacaggaat tttaagatca
      841 attatctgtg agcataaaaa caaactgcgt tttaaattag atgcatcagc aataacaatt
      901 atcctgttgg ggaaagatgg gtcttgcgga tcggtcagta ctttgtatgg aatttgctca
      961 tcaagtgttt tagggtacaa gctgtaaatg cctggtaaat ctgttagctc aactttgtaa
     1021 tcaacacctt caaatgcatt gcgtaaacta aagaagccta catgtttctc aactgtcaca
     1081 ccgggaaagt tggccacctt ttgattgaga cccgtcaata cattaaacag cgtgctttta
     1141 ccactattgg ggttaccaac caatgcaact tttagaacaa cttcactcac ttatattccc
     1201 cctctattta taatgtactt acccatactg tttctgcctc attttttctc aaactaagct
     1261 gatagcctgc aactttaatt gccattgggt cgcccaaagg tgcaactcgt tccatcaaca
     1321 ccgtttcacc tggaatacaa cccatttcca tcagtttaag ttttatctct tcatcactga
     1381 aattctgaat gacccctgcc tcgcccggct ttaactcgct taactttttt tccaatatgg
     1441 cactattaaa attaatgttg cgaaaatagt caacggcaag cacattatgg gggattaatg
     1501 tgtggtgatt tttgtcagaa aatattaata acctcgttta aaacttttca atggtcaaca
     1561 ttgctcagaa aattaacttt gcacctttgt aaaggttggg tttcattctg caaattatag
     1621 tttatgcgta atttattcct gtttttaagc cggaattata ttttcatatt gtttatactg
     1681 cttgaggcag tttgcttctt cctcattgca cagaataact cttatcagaa tgcagccaca
     1741 ctaaactcaa ccaataaagt tgtagcatct gtttattcca ctataagttc tgtaaccgat
     1801 tatttagatc tggctgaaaa caacagaagg cttgccgaag aaaatgcaca cttgcgcacc
     1861 ttgcttcctg catcatattt cgatacaacc gttgtaagaa atcataaagt ggacaccatt
     1921 cacaatcttg aatattccta tattgaggct aaaatcatta acaacagtac aaacaggcgc
     1981 aacaattata tgacacttaa caaaggttcc gatgcaggta ttgctcccga aatgggtgtt
     2041 ataacaggtc atggaattat tggtatcatc aaaaatgtat cggcacatta cagcaccgtt
     2101 acttcatttc ttcatacaca aagtaaggtg agtgtgcgtt ttaaaaacaa caactacaat
     2161 ggttcaatgg tatgggatag cgataattct cccacagaag caaccgtaat tgacattccc
     2221 cgacatgtgc cttttaaagt tggcgacaca ttggtgacta ccacattttc gccctactat
     2281 cctgagggta ttatggtggg tacggtaaaa tcttttgata ttgatgcagg aagcaactct
     2341 tatagaatta tacttaaact cagcacaagt tttaatgatg tgtcttatgt gtatgtggtg
     2401 aacaacctgc tgaaaaacga acgtaaacaa ttagaagaaa aacataaaga tgctacccga
     2461 ggtaattaaa tctgttttac gtttttttct gttgatattg ctgcaggtta tcctgcttaa
     2521 caaaatccga tttgcagggt atatcaatcc ttttgtttac ccattatttc tgttgctact
     2581 tcccgttcga ataccaaaaa cattattgct tattcttgct tttatcacag gtataagtgt
     2641 agatattttt tctgatacac tgggtatgca tgcagctgca tcggtgtttc ttggttttat
     2701 gcgtccctac atcattaagc ttctttcccc tcgtgatggc tatgactcag atgctgtaat
     2761 ctcattaaaa caaatgggca ttttgtggat ggtgtattat ttatttatca gtatttttct
     2821 gcatcatctg tttttgttct atacagaagt gttccgtttt aacgatttcg gagcaacatt
     2881 cctcagagta tgtttatcaa ccattacatc tgttgtaatt atttttatca ttttattatt
     2941 gttcgataaa acaaaagtgc aacgctaaaa ttctgaattt tgaaaacttc gcatagcaca
     3001 caacgcctcg ttttactcat tactattgca ttggtagcta ttacctatat cgggcgtttg
     3061 ttctatttac aggtaattga agataaatat aagttgtcgg cagatgaaaa cgttcttcgc
     3121 agaataattg actacccttc aagaggattg gtttatgaca gaaacggaaa gttgttggtg
     3181 tttaatcaga cagtttatga cttaatgata acaccccgtg aagtcaaaga aatggacaca
     3241 gcagaatttt gttccatcat aaaaatcagt attgaagatt tcagaaagaa atttcagaaa
     3301 gcaaaaaact attctcctta tcgttcatca atttttgaaa aacagatttc agcagaaaca
     3361 tacggaacac ttcaggaaaa actttttaag tataaaggct tcagtgttca accgcgtacc
     3421 atcagaaaat atccttacaa cactgcagca cacttgctgg gttatcttgg tgaggtagat
     3481 gaaaatgtga ctgcaaaaaa taactattac cagatgggtg attatatagg catcagtggt
     3541 attgagcaga gttatgaaaa agaattaaga ggcagacgtg gtacacgata tatgat
//
LOCUS       DAOOOL010000199         3548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3056_length_3548_cov_46.653020, whole genome shotgun sequence.
ACCESSION   DAOOOL010000199 DAOOOL010000000
VERSION     DAOOOL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_3548_cov_46.653020"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..292
                     /locus_tag="PKU82_11410"
     CDS             119..292
                     /locus_tag="PKU82_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83513.1"
                     /translation="MLIFYNTDGSILKMVKIHQTGEGSLLVYGNNLSSGQYSYSLVVN
                     NKTIKTKQMVKVK"
     gene            446..586
                     /locus_tag="PKU82_11415"
     CDS             446..586
                     /locus_tag="PKU82_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83514.1"
                     /translation="MKKSIAKKTRKAKKLIAKPAAIVAFLRDLFAEELEVLNRKNIAM
                     HN"
     gene            complement(597..1034)
                     /locus_tag="PKU82_11420"
     CDS             complement(597..1034)
                     /locus_tag="PKU82_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOZ83515.1"
                     /translation="MLNKARRYCTYQERSQQELREKLYTLGLFKKDVEETIAMMVTEG
                     YLNEERFAIAYAGGKFRIKHWGKNKIKQALKLKKVSDYCIRQALKSIADDDYLKVLNE
                     QTEIYKKKIKERNPLKKNYKVAQQLMARGFEADEVWKCILKEK"
     gene            complement(1052..2479)
                     /gene="pyk"
                     /locus_tag="PKU82_11425"
     CDS             complement(1052..2479)
                     /gene="pyk"
                     /locus_tag="PKU82_11425"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOZ83516.1"
                     /translation="MQHFNKTKIITTLGPATNSKEAIYKLIQAGADVMRINASHGDHT
                     FLKSIVNNVREINEEKQLNIALLLDLQGPKLRIGDIEGGQCELADKQQFVLTTKEMEG
                     NATKATIRYKDFASDVKKGDIILIDDGKLKLEVKQITAEQDVVTQVINGGVLMSKKGV
                     NLPFTNISIPSITEKDRADIDFAIENNIEWIGLSFTRTAQDILNLKKIIKDCGNAMRV
                     IAKIEKPEAVRNINEIIEVTDGVMVARGDLGVEIDLAQVPIIQKKIVKKCRAAGIPVI
                     IATQMMESMITNFRPTRAEANDVGNAVYDGADALMLSAETSVGKFPVETVNSMRKIIQ
                     AIEHDQEIYYRMNQPDKNSKTFISDTLCYHAAELANQSGAKAIAAMTHSGYTAFRISS
                     QRPKAGLFIFTDHKPILNTLNLVWGVRGMYYNKFESTDQTIDDIKNILKEQGYVGAGD
                     VVIHVASTPLKKRSRVNTLKINVVD"
     gene            complement(2482..2907)
                     /locus_tag="PKU82_11430"
     CDS             complement(2482..2907)
                     /locus_tag="PKU82_11430"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOZ83517.1"
                     /translation="MAKKVIKFDYSSEFDFLLAGIVCPYKDYRLCFELNRALKLSFTR
                     MKDHTLHLGKPGAKAAFSFYKSINRFGEEFYVLGNKSVQATLIPEKPAFDFFMIVKNY
                     GAYFDFNDIRNKIKQIEIVSAISELRVEELKSADNFIFD"
     gene            2969..3412
                     /locus_tag="PKU82_11435"
     CDS             2969..3412
                     /locus_tag="PKU82_11435"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOZ83518.1"
                     /translation="MSIIQQGDDYFMQEALKEARKAFDSDEVPVGAVIVHAGRIIARA
                     HNMTEKLNDVTAHAEMICFTSAAAFLNSKYLTDCVLYVTLEPCVMCAGASFWTQIPQI
                     VFGAADAKRGYQRCETNLLHPSTKIKGGVRNARLFYCRVQTVLKI"
BASE COUNT         1060 a          697 c          630 g         1161 t
ORIGIN      
        1 gctatgataa aacaacttca acaacaagta aattcattac aacaaaaatt agaagtaaca
       61 ttgaatcaac acaacaatta gctggaacat actaccacaa caagaaccat taaacgccat
      121 gttgattttt tataacaccg atggcagcat tttaaagatg gttaagattc accaaacagg
      181 agagggttct ctgttggttt atggaaacaa cctttcatca ggtcagtata gttattcttt
      241 agtggtcaat aataaaacca ttaaaaccaa acaaatggta aaggtgaagt aggggattgt
      301 ttattattca gttatatgga agttggtttt gttaaactgt ttacttaacc tgagatactg
      361 aaatacagca ggttatcaat ttataaattt ttggtacaga atttgaaata tggataagta
      421 agtataacat ttaatactcc atattatgaa aaaatcaatt gctaaaaaaa ccagaaaagc
      481 aaagaaatta attgcaaaac ccgcagccat tgttgcattt cttcgcgatt tgtttgctga
      541 agaacttgaa gtccttaacc gcaaaaacat agccatgcat aactaatctg catggcttat
      601 ttttctttta aaatacattt ccaaacttca tcggcttcaa aaccacgagc cattaattgc
      661 tgcgcaacct tgtagttttt tttaagcgga ttcctttctt ttattttttt cttgtaaatc
      721 tctgtttgct cattcaacac tttcaaatag tcatcatcag ctatagattt taatgcctgc
      781 ctgatacagt agtcagaaac tttttttagt ttcaatgcct gtttgatttt gttttttccc
      841 cagtgtttta tccggaattt tcctccggca taggcaatag caaagcgttc ttcattaagg
      901 tagccttctg taaccatcat ggcaatggtt tcttctacat cttttttaaa taatccgagc
      961 gtgtaaagtt tttcgcgcag ttcctgctga cttctttcct gataggtgca atacctgcgg
     1021 gctttgttca acacatcgct gctaaccatc cttagtccac tacattaatt tttaaagtat
     1081 ttacccgcga acgttttttc agtggcgtac tggcaacatg tatcaccaca tctcctgcac
     1141 caacataacc ttgttctttc aatatgtttt taatatcgtc aatagtctgg tctgtacttt
     1201 caaatttatt gtaatacata cctctcacac cccaaactaa attcaaggtg tttagaatgg
     1261 gtttgtggtc ggtaaaaata aaaagccctg ccttgggtcg ctgactcgaa attctgaatg
     1321 ctgtgtatcc tgaatgtgtc atagctgcaa tagcttttgc acccgactga ttggccaact
     1381 ccgctgcatg ataacacaag gtgtcagaaa taaatgtttt ggaattttta tcaggttgat
     1441 tcatgcggta ataaatttcc tgatcgtgtt caattgcctg aataattttt cgcatagaat
     1501 ttacagtctc aacaggaaat tttcctacag aagtttctgc actcagcatc aaagcatcgg
     1561 caccatcgta aacagcatta cccacatcgt tggcttcggc acgtgtaggt ctgaaattag
     1621 taatcatact ctccatcatc tgtgtggcaa tgattacggg aatgcctgcg gcacgacatt
     1681 tttttacaat ctttttctga ataataggaa cctgtgccag atcaatctct acacctaaat
     1741 caccacgggc caccataaca ccatcagtaa cttcaataat ctcattgata tttctgactg
     1801 cttcgggttt ttcaattttt gcaataaccc tcatggcatt gccgcagtct ttaataattt
     1861 tcttaagatt taatatgtcc tgtgcagttc tggtaaacga taagccaatc cattcaatat
     1921 tgttttcaat ggcaaagtca atgtctgcac ggtctttttc tgttattgag ggaatactga
     1981 tattggtaaa cggaaggtta actcccttct tcgacatcag cacaccacca tttatcacct
     2041 gtgttacaac atcctgttcc gctgtaattt gctttacttc taactttaat tttccgtcat
     2101 caatcagaat aatatcacct ttctttacat cgctggcaaa atctttataa cggatagtag
     2161 cttttgtagc attgccttcc atctcctttg ttgtgagtac aaattgctgc ttgtcggcaa
     2221 gttcgcactg tccgccttca atatcaccga tgcgcaattt aggaccttgc aaatcgagca
     2281 ggagtgcaat attcagttgt ttttcttcat tgatttctcg gacattattt acaattgact
     2341 ttaaaaaagt atggtcgcca tgcgaagcat ttatacgcat aacatcggca cctgcctgaa
     2401 tgagtttgta aatggcttct ttggaattgg tggccggccc aagggtggta ataattttag
     2461 ttttattaaa atgttgcata atcagtcgaa gataaaattg tctgccgact tgagttcttc
     2521 aacacgaagc tcactaatgg cagaaactat ttctatttgt tttattttgt tgcgaatgtc
     2581 attaaagtca aaataggctc cgtaattttt tacaatcata aaaaagtcga aggcaggctt
     2641 ttcgggaata agtgtggcct gaacactttt atttccaagt acataaaact cttcgccaaa
     2701 acgattgatg gatttgtaaa aagaaaaggc tgcttttgcg cccggcttgc caagatgcaa
     2761 ggtgtgatct ttcattctgg tgaaagaaag ttttaatgca cggtttagct caaagcaaag
     2821 acgatagtct ttatacggac atacaattcc ggcaagcaga aagtcaaact cactgctgta
     2881 atcaaacttt ataaccttct ttgccaagag aaacagattg tttatttaaa acggatattt
     2941 tcgctgctaa attagattaa tttgcagtat gagcataatt cagcaaggcg atgattattt
     3001 tatgcaggaa gcattaaagg aagcaagaaa agcttttgac agtgatgaag ttcctgtggg
     3061 tgcagtaatt gttcatgcag gacgaattat tgcccgcgca cataatatga cagaaaagct
     3121 gaatgatgtt acggcacatg cagaaatgat ttgctttact tcagcagcag catttttaaa
     3181 ctcaaaatac ctgaccgatt gtgtacttta tgtaacgctt gaaccttgtg tgatgtgtgc
     3241 cggagcatcc ttttggacac agatacctca gattgttttt ggtgctgccg atgccaaacg
     3301 aggctatcag cgttgcgaaa ccaatctgct gcatccttca acaaaaataa aaggaggcgt
     3361 cagaaatgca agattgtttt attgtcgagt tcagacagtt ttaaagatct gaatcgtgcc
     3421 aacaaatgcc gttatgtgag aaggttttta aacaaaccat taaccaactc catgctgcag
     3481 gcaattaatt tctagattct tttttttcga aaaaattctt tcagtaagtt gctacaggca
     3541 tcttcaag
//
LOCUS       DAOOOL010000200         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3060_length_3545_cov_31.115759, whole genome shotgun sequence.
ACCESSION   DAOOOL010000200 DAOOOL010000000
VERSION     DAOOOL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3060_length_3545_cov_31.115759"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="PKU82_11440"
     CDS             <1..1654
                     /locus_tag="PKU82_11440"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOZ83519.1"
                     /translation="GNNHCYKLKFKPRRHEDLTFTGEMFITDTTWAVKKVNMRISNKA
                     NINFVEDCAVVKEYEFVDSTMWMLSKDLLVIEFAARDKGLGLIGRKSTSYKNFDLNKP
                     IPPNVMKGSQDVVVLDSALYKDNSFWDTARHDSLNEREKKIYTMVDTIKSLPAYKTYV
                     DVITLFVTGYKTLGKVDLGPYFTVFSFNEIEGYRFRLGGKTNQDFSKHLRLEGFVAYG
                     TKNEQFNYSFGTRYLFTTKPRMGVGFKYRHDVMQLGQSDNAFQDDNILASLFRRSASN
                     KLTYTESQKIYFEKEWLSGVSGRITFEHNEFIPLGKLDYRYYTNENESNIRDKINTSD
                     ISAYFRFAYREKFVESKHGRVSLGSKYPVLQAVYTQGLKGVLRSDFDYQKLIVKVDDY
                     LYTAPIGYLYYVLEAGKVWGKLPYPLLEVHRGNESYFYDYLSFNLMNYYEFVSDQYFA
                     FYATHHFEGFFLDKIPLLKKLKWREVASLRGVYGSMSQKNHDLMVAPTYNTLSKKPYL
                     EAGFGVENILKIIRVDFIYRLSYLDNPDISKFGIRGSLQLTF"
     gene            1691..2416
                     /gene="lptB"
                     /locus_tag="PKU82_11445"
     CDS             1691..2416
                     /gene="lptB"
                     /locus_tag="PKU82_11445"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOZ83520.1"
                     /translation="MLLRAENLVKIYKKRTVVNNVSIQVAQGEIVGLLGPNGAGKTTT
                     FYMTVGLIKPNSGKIFLDDNDITALPMYKRAQSGIGYLAQEASVFRKLSVEDNIKAVL
                     EMTKLSQEEQDAKTEELLNEFGLQRIRKSRGDLLSGGERRRTEIARALAVSPKFILLD
                     EPFAGVDPIAVEDIQSIVAKLKEKNIGILITDHNVHETLTITDRAYLLYEGKILKAGT
                     AEDLANDEQVRKVYLGQNFELRN"
     gene            complement(2428..3222)
                     /locus_tag="PKU82_11450"
     CDS             complement(2428..3222)
                     /locus_tag="PKU82_11450"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOZ83521.1"
                     /translation="MGVFLLVTSKELMMFSFAAFLLAFAFLFFIFYRSKREAEVQIEK
                     SRLQLQVAQVEMKALRAQVNPHFMFNALNSIYILIADKEMQKAGNYVLKLGTLIRKIL
                     ENSNHQNISLKDDCEVIDLYIQVECMRLNYSFNYQLTINETIDNESVMVPPMIIQPIV
                     ENAIWHGLKNRKDGGLLTINVDSTKDELVFEIKDNGCKADARMSEDEKLRNAKRKSMG
                     MQITKERLDIINQSNNANARIIEKEIIGGDGSYQGKVVKLYLPLLD"
     gene            complement(3331..>3545)
                     /locus_tag="PKU82_11455"
     CDS             complement(3331..>3545)
                     /locus_tag="PKU82_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83522.1"
                     /translation="SFRISRQGYERRVIRFNKLKGNNVIFMDISLRKITAEEEAKIQH
                     KEEKKHKTKSKRKKYARPGKRIFLPF"
BASE COUNT         1145 a          622 c          637 g         1141 t
ORIGIN      
        1 gggtaacaat cattgctata aacttaagtt taaaccacgc agacacgaag accttacttt
       61 tacaggtgag atgtttatta ccgacactac ttgggcagtc aaaaaagtaa acatgcgtat
      121 ttccaataaa gccaatatca actttgtgga agactgtgct gttgtaaaag aatatgaatt
      181 tgtggacagc accatgtgga tgctttcaaa agatttgttg gtgatagaat ttgctgctcg
      241 cgataaagga cttggtctca taggacgaaa gtcaacatct tataaaaact ttgatttgaa
      301 caaacctatt ccaccaaatg taatgaaagg ctcgcaggat gtggttgtac ttgatagtgc
      361 actttacaaa gacaatagtt tttgggatac ggctaggcat gattcgttaa acgagcgtga
      421 aaagaaaatt tacaccatgg ttgacaccat taaatcatta cctgcttata aaacttatgt
      481 tgatgtaatt acactttttg ttaccggata taaaacattg ggcaaagttg atttgggacc
      541 gtatttcaca gtatttagtt ttaatgaaat tgaaggttat cgttttcggc ttggtggaaa
      601 aacgaatcag gattttagta aacatctgcg tttagaaggc tttgttgcct atggtacaaa
      661 aaacgaacag ttcaactata gttttggcac tcgttactta tttacaacaa aaccacgcat
      721 gggtgttggc tttaaataca ggcacgatgt gatgcaactt gggcaaagtg ataatgcatt
      781 tcaggacgat aatattttgg catcgctttt caggcgatcg gcctccaaca aattaactta
      841 tactgaaagt caaaaaattt attttgaaaa agaatggctt tccggtgtca gtggacgaat
      901 cacttttgaa cacaatgagt ttataccact aggaaaatta gattatcgtt actatacaaa
      961 tgaaaatgaa agcaacatca gagataaaat taacacgtct gatatttctg cttacttcag
     1021 gtttgcctat cgtgaaaaat ttgttgaaag caagcatgga cgtgtaagtc ttggaagtaa
     1081 atatccggtg ctgcaggctg tatatacaca aggattaaaa ggtgttttac gaagtgactt
     1141 tgattatcag aaactgattg ttaaggtgga tgattattta tatactgcac caatcggcta
     1201 tctgtactat gttttagaag ccggaaaagt ttggggtaaa ttaccttatc cgttactgga
     1261 agtgcatcgc ggcaatgaat cctattttta tgattatcta tctttcaacc tgatgaatta
     1321 ttatgaattt gtcagcgacc aatattttgc attttatgca acacatcatt ttgaaggatt
     1381 cttcttagat aaaattccat tgcttaaaaa attaaagtgg cgcgaagtgg caagccttcg
     1441 tggtgtttat ggcagcatgt cgcaaaaaaa tcatgattta atggttgccc ctacctacaa
     1501 tacactttct aaaaaaccct atcttgaggc aggttttgga gtagaaaata ttttaaagat
     1561 aattcgtgtt gattttattt atcgtctcag ttatttagac aatcccgaca tttctaaatt
     1621 cggaatcaga ggaagtttgc aattgacatt ctgatagctg ttatctttgc accatattga
     1681 attgacaaaa atgttgttac gcgcagaaaa cttagttaag atttataaaa aacgaaccgt
     1741 tgttaacaat gtgtctattc aggtagcaca aggtgaaatt gttggtttgc ttggccccaa
     1801 tggtgccggt aaaaccacaa cgttttatat gactgttggc ttgataaaac ccaatagcgg
     1861 taaaattttt cttgatgata atgatattac agccttgcca atgtataaaa gggcacagtc
     1921 cggtattggc tatttagctc aggaagcttc tgtttttcgc aaattaagtg ttgaagataa
     1981 tattaaggct gttttggaga tgacaaaact ttcgcaagaa gaacaggatg ccaagacaga
     2041 agaacttctt aatgaattcg gtttacagag gataagaaaa agccgaggtg atctattatc
     2101 cggtggcgaa cgcagacgaa cagagattgc acgtgctttg gctgtgagtc ctaaatttat
     2161 tctgctcgat gagccatttg caggtgttga ccccattgct gttgaagaca tacaatcaat
     2221 tgtagcaaag cttaaagaaa aaaatatcgg aattttaatt actgatcaca acgttcacga
     2281 aacactaact ataactgacc gcgcctatct gctgtacgaa ggtaaaattt taaaagccgg
     2341 tactgccgaa gaccttgcta atgatgagca ggttcgaaaa gtatatctcg gacagaattt
     2401 tgagcttagg aattaaatag caagatttta atccaaaaga ggaagatata gtttcacaac
     2461 cttaccctga taactgccat caccaccaat aatttctttt tcaattattc tggcattggc
     2521 attgttgctt tgattgataa tatctaaacg ctccttagtg atttgcattc ccattgattt
     2581 tcgtttggca ttgcgcagtt tttcatcttc tgacattcgg gcatctgctt tacaaccatt
     2641 atcctttatt tcaaaaacta attcatcttt tgtgctgtca acattaatgg taagaagacc
     2701 accatctttt ctgtttttta aaccatgcca aattgcattc tctacaatag gctgaataat
     2761 cattggcgga accatcacac tttcattatc tatagtttca ttaattgtca gttgataatt
     2821 aaaactataa ttaagcctca tacattcaac ctgaatataa agatcaataa cttcacaatc
     2881 gtccttcaaa gaaatattct gatggtttga gttttcaaga atcttacgga ttaatgtgcc
     2941 taactttaaa acatagtttc ctgccttttg catttcctta tcagcaatta aaatgtaaat
     3001 gctgtttaat gcattgaaca taaaatgtgg attcacctgt gcacgcagcg ccttcatttc
     3061 tacttgagct acctgaagtt gcaatctgct tttttctatc tgaacttctg cctcgcgttt
     3121 tgaacggtaa aagataaaaa acaaaaaagc aaacgccaat aagaaagcag caaagctaaa
     3181 catcattagc tccttggagg ttaccaataa aaaaacaccc atataaaaac ttaactcgga
     3241 ttaaaacttg taaccgatga actacacggc tgaaatctat tggccaaaat aactaaaatt
     3301 gaataaaaat aataccttta ttaaaaaaac ttagaaaggt aaaaatatac gttttcccgg
     3361 cctggcatat ttttttcgtt ttgactttgt tttatgcttt ttctcttcct tatgttgaat
     3421 tttagcctct tcttctgctg taattttcct gagtgaaata tccataaaaa tcacattatt
     3481 ccctttcaat ttattaaacc tgataactcg tctttcatat ccttgcctcg atatcctgaa
     3541 actca
//
LOCUS       DAOOOL010000201         3544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3061_length_3544_cov_34.435082, whole genome shotgun sequence.
ACCESSION   DAOOOL010000201 DAOOOL010000000
VERSION     DAOOOL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_3544_cov_34.435082"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PKU82_11460"
     CDS             complement(<1..1103)
                     /locus_tag="PKU82_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOZ83523.1"
                     /translation="MYQTWFLDYASYVILERAVPYLDDGLKPVQRRILHSMWEMEDGR
                     YNKVANIIGNTMKYHPHGDASIGDALIQIGQRDLLIDMQGNWGNIYTGDAAAASRYIE
                     ARLSKFALDVIYNPKTTVWQLSYDGRNKEPVTLPVKFPLLLASGIEGIAVGLACKILP
                     HNFCELIQGSIDVLRNKKTNILPDFATGGIADFRNYNEGLRGGRIKVRAVIEQKDKKT
                     LLIKQIPFGTTTSSLIDSILSANDKGKIKIKKVEDNTAAEVEIAIYLHPDSSEDIEKT
                     IEALYAFTDCELSIAPNSCVIRDGKPEFLNVNDILKESTFNTLELLKRELEIELSELM
                     EDWHFSSLEKIFIENRIYRKIEECETWEAVIQA"
     gene            complement(1234..3096)
                     /locus_tag="PKU82_11465"
     CDS             complement(1234..3096)
                     /locus_tag="PKU82_11465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOZ83524.1"
                     /translation="MSKKATHNYTEDSIQSLDWKEHIRLRPGMYIGKLGDGSSRDDGI
                     YVLLKEVIDNSIDEYVMGYGKQIDVTVENGSVTVRDYGRGIPLGKVVDCVSKINTGGK
                     YDSQAFQKSVGLNGVGTKAVNALSTYFKVQSVREGKTTIAEFERGELKKQQKEKSCDL
                     ADGTLISFIPDNEVFIDYKFNSEFIERMLNNYAFLNVGLKLNFNGKEFFSQNGLLDLL
                     KANLSEEVLYPAVHLKDNDIEIAFTHTPSYGEEYFSFVNGQHTTQGGTHLAAFREAIV
                     KTVREFYKKEFDSSDVREGIEAAVSIRIQEPVFESQTKTKLGSQNTYPNGPSLRNWIN
                     DFVKTNLDNYLHKHPETAEIWLKKIQQSERERKEIAGIKKLANERAKKANLHNKKLRD
                     CRSHLNSKSPERFESTLFITEGDSASGSITISRDVNTQAVFSLKGKPLNCFGLTKKIV
                     YENEEFNLMQNALNIEDGLDELRYNRIVIATDADVDGMHIRLLLLTFFLQFFPELVKN
                     NHVYILQTPLFRVRNKKETIYCYSEQERVAAIEKLGSKPEITRFKGLGEISPDEFKNF
                     IGRKMRLEPVRIEGEGSVQHLLEYYMGKNTPERQEFIIGNLKLEEELEPEIAEA"
BASE COUNT         1007 a          676 c          618 g         1243 t
ORIGIN      
        1 attgcttgta ttactgcttc ccaggtttcg cattcttcaa ttttacgata aatacggttt
       61 tcgataaata ttttttcgag tgaagaaaaa tgccaatctt ccatcaactc actcagttca
      121 atttcaagtt cgcgtttcag taattcaagt gtattgaatg ttgactcttt aagaatatca
      181 tttacattca gaaattccgg tttgccatct cgaataacac aggagttggg tgcaatgctt
      241 agttcacaat cagtaaatgc ataaagtgct tctattgttt tttcaatgtc ctcgctggag
      301 tcaggatgca gataaatagc aatctcaact tctgcagcag tgttgtcttc tacttttttt
      361 atcttaatct ttcctttgtc attagccgaa agtatagagt caatgagcga agatgttgta
      421 gtgccgaatg gaatctgttt aatcaggagt gtctttttat ctttctgttc aatgactgca
      481 cgtaccttta ttcttccacc acgcaggcct tcattgtaat ttctgaaatc tgcaatacct
      541 cctgtagcaa aatcaggaag tatatttgtt tttttatttc gtaaaacgtc aatagagcct
      601 tgtattaatt cacaaaaatt atgtggtaat attttacagg ccaaaccaac ggcaatacct
      661 tcaatccctg aagctaacaa caatggaaat tttacaggta gtgtaacagg ctctttattt
      721 cttccatcgt atgaaagttg ccatacagta gttttaggat tgtaaattac atcgagtgca
      781 aactttgata aacgtgcttc aatataacgt gaggcagcag cggcatcacc ggtgtatata
      841 tttccccagt taccctgcat atcaatcagc aaatcgcgct gaccaatctg aatcagtgca
      901 tcaccaattg aagcatcacc atgcggatga tatttcatgg tgttaccaat gatgttggcc
      961 actttattgt atcttccatc ttccatttcc cacatgctgt gcaggattct tcgttgaaca
     1021 ggttttagtc cgtcatcgag ataggggaca gcacgttcga ggattacata cgaagcataa
     1081 tctaagaacc aggtctgata catatttgaa atatgtgttg ccggttgttc gattatagtg
     1141 tttacaggag attttacagg tttaatattt tttttgctca ttagttactt tttttgtgtc
     1201 gggtgacggt gaaataaatc aattatattt caattatgct tctgcaattt caggttctaa
     1261 ctcttcttca agtttcagat tgccgataat aaattcctgt ctttccggag tgtttttacc
     1321 catgtaatat tctaatagat gttgaacgga gccttcgcct tcaatacgca ccggttcgag
     1381 gcgcattttg cgacctatga aatttttaaa ctcatcaggc gaaatttctc caagaccttt
     1441 aaagcgtgtt atctcaggtt tactacctaa tttttctata gcagcaacac gctcctgctc
     1501 gctgtagcaa tagattgttt ctttcttatt ccttactctg aaaagcggtg tttgtagaat
     1561 ataaacgtga ttatttttta ccaactcagg aaaaaattgc agaaagaaag tgagtaacaa
     1621 caacctgata tgcattccgt caacatcggc atcggttgca attacgatac ggttgtagcg
     1681 taactcgtca agtccgtctt caatatttaa tgcattttgc atcaggttaa actcttcgtt
     1741 ttcgtaaaca atttttttgg tgagtccaaa gcaattcaga ggctttcctt ttaagctgaa
     1801 aacagcttgt gtgtttacat cacgcgaaat ggttatagaa ccacttgctg agtcaccctc
     1861 tgtaataaac agtgttgact caaatctttc aggagatttg gagttgaggt gtgagcgaca
     1921 atcacgaagt tttttattgt gcaggtttgc cttcttagca cgttcgttag caagtttttt
     1981 tatacctgca atttcttttc tttcacgctc gctttgctga atttttttta accagatctc
     2041 agcagtttca ggatgtttgt ggaggtagtt gtcgaggttg gttttaacga agtcgttaat
     2101 ccagttacgt aatgaagggc cattaggata tgtattttgt gagcctaatt ttgtttttgt
     2161 ctgcgactca aaaacaggct cctgaatacg aatacttaca gcggcctcaa taccttcgcg
     2221 cacatcactt gaatcaaatt cctttttgta aaactcacgt accgtcttaa caattgcttc
     2281 tctaaatgca gcaagatggg ttccgccttg tgtagtgtgt tgtccgttta caaaagaaaa
     2341 atattcttct ccataacttg gtgtatgggt aaatgcaatt tcaatgtcat tgtcttttaa
     2401 atgcacagca gggtagagca cttcttcact caggtttgcc ttcagcaaat caagtaatcc
     2461 attttgtgaa aagaattctt tgccattgaa attcagcttt aatcctacgt tcagaaaagc
     2521 ataattattc aacatccttt caataaactc actgttgaat ttataatcaa taaacacttc
     2581 attgtcggga ataaaactga tgagtgtgcc gtcagcaagg tcgcatgatt tttctttttg
     2641 ctgttttttt aattcacctc tttcaaattc tgcaatggta gttttgcctt cacgcacact
     2701 ttgcacttta aaataggtag aaagagcatt cactgcttta gtacctacac cattaagtcc
     2761 aacagatttc tgaaatgcct gtgagtcgta ttttccaccg gtatttattt tagaaacaca
     2821 gtcaacaact tttcctaatg gaataccacg accgtaatca cgcaccgtaa cactgccatt
     2881 ttcaactgta acatcaatct gctttccata tcccatcaca tattcatcaa tggaattatc
     2941 tatcacctct ttcaacaaaa cataaatacc atcatcgcgt gaagaaccat cacctaattt
     3001 gccaatatac attcccggtc tgaggcggat atgctctttc cagtctaaac tttgtatgct
     3061 gtcttcggtg tagttatgtg tagctttttt tgacatgctt cgattatttt cttagttctt
     3121 taaaattaaa taaattggct gcaatgcaaa ccattgcccg attgctgaaa ttatacttta
     3181 aatacagacg gttctgtttt aacaaaaatg ccttttaaac aagtgtctgt taaaaaattc
     3241 agggtgcaaa gataagaggt gtgactgctt catttttcat ttcttcaact gttgttaaaa
     3301 agtattactg ctgaatttta ggaggttatc ggtttagtaa atagtttgca tacacatgtt
     3361 gtttttgtca aaaaactgtt tatgaagctg taattatatg ggagattcag tttcttcaag
     3421 tgtttgttcc aattacattt ttattttatg gtagaaattt ttttataact caataaaatg
     3481 aacaaacaga cgttttgttt taatttgtaa tttagagcaa tataaaaaat ggaaaagcaa
     3541 cgca
//
LOCUS       DAOOOL010000202         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3078_length_3528_cov_59.565793, whole genome shotgun sequence.
ACCESSION   DAOOOL010000202 DAOOOL010000000
VERSION     DAOOOL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_3528_cov_59.565793"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PKU82_11470"
     CDS             complement(<1..1199)
                     /locus_tag="PKU82_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HOZ83525.1"
                     /translation="MKKFFTLFLAACFATAVNAQNFNISLLGTLPYPNDDLANIGGWV
                     SPSGEEYALVGSESGLSIVDVSNPANPTEVILVPGPQSQWREVKTYDHYAYVTTEGGG
                     GLQIVDLSQLPNAAPAIDWTGNGAISGQLDNIHSLHIEDGYVYLHGSNLFQGATIIAD
                     IATNPLSPNYVGHTPGAYVHDGYARGNYYYGCHIYNGDFTIYDVTNKANPIPLGIKVT
                     PGQFTHNAWLSTNSQYLFTTDEVSDSYLTAYDISNPANIIEMDRIQITPGSGSIVHNT
                     HIIQKNGADYAVTSWYKDGVVITDVTRPQNMVNVGWYDTYTQGAGDGFSGDWGVYPYL
                     PSGNLVVSDINNGLFVLGPTYQRACYLEGVVTDSVTGNPIFNATVKVLSTNITKNTKA
                     NGEYKTGT"
     gene            complement(1340..2497)
                     /locus_tag="PKU82_11475"
     CDS             complement(1340..2497)
                     /locus_tag="PKU82_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOZ83526.1"
                     /translation="MRHQTLLNHITGIIAGFDFSVPLHHYLKNHFRQNKQLGSRDRKI
                     ISSGCYAFYRCALLLPHKSVEEKLALSFFITNETNLLSKYLFEKYYITNNESHNLSEK
                     LEFNKQQGGINPDIAFPFLNLLSEEIDKEVFTASMFQQPLVWIRIRKGFEQHVSDELK
                     SKEITPLQIEGKTLGFAPAAPLTTLESYLKGYFEIQDLSSQQTLSLLPDNCGQRMWDC
                     CSGSGGKTLLLADRFPNASFFLTDIRSSSLQNAETRLKKATVRNYKTLQADLTSDLNF
                     NEKFDVIIADVPCSGSGTWCRTPENFWNFDKHKIKDYAELQFTIAKQATMHLKSNGLF
                     LYLTCSAFAEENEENTKRIAKHCNLSIVVQKFFHGYSLQADTMYGCVMMGF"
     gene            complement(2501..>3528)
                     /locus_tag="PKU82_11480"
     CDS             complement(2501..>3528)
                     /locus_tag="PKU82_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83527.1"
                     /translation="IYRKIEECETWEAVIQAIDKGLNPFKKLLKREVTEDDIVKLTEI
                     KIKRISKFDAKKADEHIKALNDKIKVVKDNLKNLTPFAIAYFKELLKKYGKGKERKTV
                     ISEEGFEQIEVRKVVINNAKLYVSREEGFAGYGLKKDELVSECSDIDDVLAITGDGKF
                     VVNRISDKSYYGKDIRFIKVFEKNDESTVYNMVYRDGPRGDLFIKRFSIGGITREKIY
                     DLTKGTENSKIIYLGISGTDNAPTIQIVLKPRPKLKNTVFNVNFNEMLIKNRSAVGNI
                     LTKFPVHKITEVGKGKSEASAVKSSEPSKKNEKESEPELFEFPKEKNNTEQAKTPAQK
                     VQMKMEI"
BASE COUNT         1008 a          652 c          665 g         1203 t
ORIGIN      
        1 gttcctgttt tatattctcc gtttgctttt gtgtttttag taatgtttgt agatagaact
       61 tttaccgttg cattaaaaat agggttacct gtaactgaat ctgtcaccac accttcaaga
      121 tagcaggcac gctgataggt aggtcctaaa acaaaaagtc cgttgttgat gtcagatact
      181 acaagatttc ctgagggcag atagggataa acaccccaat cgccactgaa accatcacct
      241 gcaccttgtg tataggtatc gtaccaacca acgttaacca tattttgtgg acgtgttaca
      301 tcagtaatca ccacaccatc tttataccat gaagtaactg catagtcagc accatttttt
      361 tgaatgatgt gcgtgttgtg aacgatagaa cctgagccgg gagtgatttg aattctgtcc
      421 atttcaataa tgttggcagg atttgaaatg tcataggctg tcaagtagga atcagaaact
      481 tcatcagtag taaacaaata ctgactgttg gtagaaagcc atgcattgtg tgtaaactgt
      541 cccggtgtta cttttattcc caatggaatg ggattggctt tattggtaac atcataaatg
      601 gtaaaatcgc cattataaat atggcaaccg taataatagt tacctctggc atagccgtca
      661 tgcacatagg cacctggtgt atgtccaaca taatttggac ttagtggatt ggttgcaata
      721 tctgcaatga tagttgcacc ctgaaataaa tttgagccat gcagataaac atagccatct
      781 tcaatatgca atgagtgaat gttatctaac tgaccactga tggcaccatt acctgtccag
      841 tcaattgcag gtgctgcatt aggaagttga gagaggtcaa caatttgtag tccgccacca
      901 ccttcagttg ttacataagc ataatgatcg taagttttta cctcgcgcca ctgcgattgt
      961 ggtccgggaa ctaaaattac ctctgttggg tttgcaggat tgctgacatc aacaatagaa
     1021 agtccggatt ctgaacctac gagtgcatat tcttcgcccg atggtgatac ccatccgcca
     1081 atattggcaa ggtcgtcatt ggggtagggc aatgtgccaa gtaaagaaat gttgaaattt
     1141 tgtgcattaa ctgctgtagc aaagcatgca gctaaaaaga gtgtgaagaa ttttttcatg
     1201 tttatttttg aaaagaccga caaagctatc aaaatagcaa tacatcagta gtgtgatgca
     1261 attttaattt aacatcaatt aacaggtgaa tgggtggggg gataaatgag tttttaggtg
     1321 ataattacga taatttctat caaaatccca tcatcacaca accatacatt gtatctgctt
     1381 gtaggctgta tccatgaaaa aatttttgca ccacaatact aagattgcaa tgttttgcaa
     1441 tacgtttggt attttcttca ttttcttcgg caaatgcaga gcatgttaaa tacagaaaca
     1501 ggccgttact ttttaaatgc attgtagctt gtttggcaat ggtaaattgc aactcggcat
     1561 agtccttaat tttatgttta tcgaaattcc aaaagttttc aggtgtgcga caccacgtgc
     1621 cggagccact gcatggcaca tcagcaataa tcacatcaaa tttttcatta aagtttaagt
     1681 ctgatgtaag gtcggcttgc aatgttttat aatttctgac agtagctttt tttaatcttg
     1741 tttctgcatt ttgaagtgaa gaactgcgta tgtcggtaag aaaaaaagac gcatttggaa
     1801 aacggtcggc cagtaacaaa gttttgccac ctgagccact gcaacaatcc cacatacgtt
     1861 gcccgcagtt atcgggtagc aacgatagtg tttgttgtga tgataaatct tgaatctcaa
     1921 aatagccttt caggtatgat tcaagtgttg taagaggggc agcaggggca aagcccaacg
     1981 tttttccttc aatttgcaat ggggtaattt cttttgactt caattcatct gaaacatgtt
     2041 gctcaaaacc ttttcgtatt cttatccaca ctaaaggttg ttgaaacatt gatgcagtaa
     2101 atacttcttt atctatttct tcggaaagta aatttaagaa cggaaaagct atatctggat
     2161 ttattcctcc ctgttgttta ttaaattcca gtttttcaga aagattatgt gattcgttat
     2221 ttgtgatata atatttttcg aataaatatt tgcttaacag gtttgtttca ttggttataa
     2281 agaaactcaa agccaatttc tcttcaacac ttttgtgggg tagaagtaaa gcacagcgat
     2341 aaaaggcata gcatccagat gaaattattt ttctgtcgcg gctgccgagt tgtttgtttt
     2401 gtctgaaatg gtttttcaga tagtgatgta gaggtacaga aaaatcaaaa ccggcaatga
     2461 tgccggttat atgattgagt aatgtttgat ggcgcatgaa tcagatttcc attttcatct
     2521 gcactttttg tgcaggtgtt tttgcctgct cagtattgtt tttttctttt ggaaactcaa
     2581 agagttcagg ctcactttcc ttttcgtttt tttttgaagg ctcagagctt ttcactgcag
     2641 aagcttctga ttttcctttg ccaacttctg tgattttgtg aacaggaaat ttggtgagga
     2701 tatttccaac ggcagaacgg ttttttatca gcatctcgtt aaaattcacg ttaaacaccg
     2761 tattttttaa ttttggtcgt ggcttcagca caatctgaat ggttggtgca ttgtcagttc
     2821 cggaaatgcc gagatagata atctttgaat tttcagttcc ttttgtaagg tcataaattt
     2881 tttcgcgtgt gatgcctccg atagaaaaac gtttgataaa caaatcacct cgaggtccat
     2941 ctctgtaaac catattgtaa acggtgcttt catcattctt ttcaaacact ttgataaagc
     3001 gtatgtcttt accgtaatag cttttgtcag atatacggtt aactacaaac tttccatcac
     3061 cggttattgc cagtacatca tcaatgtctg aacattcact aacaagttca tcttttttca
     3121 gaccatagcc tgcaaatcct tcttcacggc ttacgtatag cttagcatta ttgataacaa
     3181 cttttctaac ttcaatctgc tcgaatcctt cttcgctgat tacagttttt cgctccttac
     3241 ctttaccata ttttttaagc aactctttaa aatatgcaat agcaaaaggt gtcagatttt
     3301 taagattatc ttttacaact tttattttgt cattaagtgc tttaatatgt tcatctgcct
     3361 tcttagcatc aaatttagaa atacgtttga ttttaatttc agtaagtttt acaatatcat
     3421 cctcagtgac ttcgcgcttc aataatttct taaatgggtt cagtcctttg tctattgctt
     3481 gtattactgc ttcccaggtt tcgcattctt caattttacg ataaatac
//
LOCUS       DAOOOL010000203         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3119_length_3488_cov_63.292747, whole genome shotgun sequence.
ACCESSION   DAOOOL010000203 DAOOOL010000000
VERSION     DAOOOL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3119_length_3488_cov_63.292747"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PKU82_11485"
     CDS             <1..1048
                     /locus_tag="PKU82_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOZ83528.1"
                     /translation="RTFFDTRLALFGYIITILILGSIASNGFDFVFTQICAGMITIFS
                     IKDMRNRSHVFWAVLFVYIGYFICYTSLEIIHAGNMVKVNWENSFWLLANVLLTLFAY
                     PLIYFLERIFKVTSDISLIELADSNNPLLHELSIKAPGTFQHSLQVANLAESAMFKIG
                     GNALLVRVGALYHDIGKMDMPLYFIENQVTQVNPHDELSFDESAGIIISHVIKGIEKA
                     RKYNLPDLVIDFIRTHHGTSRVQYFYESYLKNYPDKIIDENAFHYPGPKPFSRETAVL
                     MMADSVEAASRSLKKHDSESINKLVEDIIDNQIAHGQFSNCNITFRDITELKKLFKKM
                     LGSIYHVRVEYTVA"
     gene            complement(1056..2237)
                     /locus_tag="PKU82_11490"
     CDS             complement(1056..2237)
                     /locus_tag="PKU82_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83529.1"
                     /translation="MRRKLKFRLAKLWLLTVVLLALFAPFIANEKPFFGNDNLTSDYD
                     LSFGIESKGNNQILRPIPYSPGQPRQNDIALLSPFQKSYYTNNTGQQIELPLRFHHWL
                     GTDAIGADVAAGIVFGLRHSLIIALFSMIFSIVISLIAGICSGYFADSGIYLNRIKFV
                     SLIIFLFLVWFYGFYTFSMEWHNLFQQFSLINLIDLSPSFILFLLLTIALILFWKLSN
                     KRKIKATNHIHLNPDFWISLLTQVMVAVPQLIIIIALSSLFQPSIITLIIIFGSVLWV
                     EPSRMIRAEVQRLRSEGFVDAAIVTGFSFRQIALRHLIPNLIPVVKPVFLYGMAAVIL
                     SESGLSFIGLGVNPGTVTLGGLMAQAKENIDAWWLILFPGSCIFFTILSLSALSEKNK
                     S"
     gene            complement(2234..>3488)
                     /locus_tag="PKU82_11495"
     CDS             complement(2234..>3488)
                     /locus_tag="PKU82_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83530.1"
                     /translation="QRLGYDKPLFYFSVISLAEYGWQQYLPSEKQQKSYNHLLRNFGC
                     GSEVFIFYKKINAARTAITTSFASATDTAIRQTASNAANSLFQIETQVKLSKNDSILD
                     SFNNYATILKLNSLANSILHSYNAMQTKKSSWKKWVPAVQLNAQNRFHDWFFGTDKNS
                     GIIHLNLGQSLYTGRSIGSLLKEKIFWSLLLTVLAIIIAFISGLLISIWLAYTKNKIA
                     SRIINMITVLFYSLPVYFAGTALLYFFSNPDFLQIFPSSGVMPIGGYSSSASFSEKFI
                     GTLPYLILPLICYTYSLWAFVQRTSASLISAEFKKPYALMAQAKGLTKKEVVIKHIFP
                     NIISPFVALLANMFPAVIGGSVIIESLFSIPGMGLELIHATTTRNYPFISAVILITGL
                     ITILLYLLNEWIQHYVDPRTKTVQP"
BASE COUNT         1174 a          563 c          654 g         1097 t
ORIGIN      
        1 acgtacattt tttgatacac gacttgcatt gtttggttat atcatcacaa ttttaatatt
       61 aggatcaatt gcatcaaacg gttttgattt tgtgtttaca caaatctgtg ccggtatgat
      121 aactattttc agtataaaag atatgcgcaa ccgctctcat gttttctggg cagtgttgtt
      181 tgtttatatc ggttatttta tttgttatac cagcctcgaa attattcatg ccggaaatat
      241 ggttaaagta aattgggaga attctttctg gttgcttgct aacgtgttgt taacactttt
      301 tgcttatcca ttaatatatt tccttgagcg tatttttaaa gtgacttctg atatttcttt
      361 gatagaactt gcagacagta acaatccatt gttacatgag ttgagtataa aagctcccgg
      421 aacatttcag cattcactgc aggttgctaa tcttgctgaa tctgcaatgt ttaaaatcgg
      481 tggcaatgca ttgttggtca gagtaggtgc attgtatcat gacattggta aaatggatat
      541 gccgctttat tttatcgaga atcaggttac gcaagtaaat ccacacgatg aattgtcttt
      601 tgatgaaagt gcgggtatca tcatcagcca tgtgattaaa ggaatagaaa aagccagaaa
      661 gtataatctt cccgatctgg ttattgactt tataagaaca catcacggaa cctcacgtgt
      721 acagtatttt tatgagtcgt atcttaaaaa ttatcctgac aagattattg atgaaaatgc
      781 atttcattat cccggcccaa aacctttcag cagagagact gccgtattga tgatggcgga
      841 ttctgttgaa gctgcatcac gaagtcttaa gaagcatgat tccgaaagta tcaataagtt
      901 ggtagaagat attatagata accaaattgc acacggacaa ttttctaact gtaatattac
      961 tttcagagat attactgagt tgaaaaaatt attcaagaaa atgcttggaa gtatctatca
     1021 tgtacgtgtt gaatatacgg tagcatgata aaatgtcaag atttattttt ttcgcttaag
     1081 gctgacaagc tcaaaattgt aaagaaaata catgagcccg gaaaaagtat cagccaccag
     1141 gcatcaatat tttcttttgc ctgagccatc agtccaccta aagtaacggt tccgggattt
     1201 acgcctaatc caataaatga taatcctgac tcactcaaaa taacggctgc cattccatat
     1261 aagaatacag gttttacaac aggaattaaa ttggggatta aatgcctcaa agcaatttgt
     1321 ctgaatgaaa atccggtaac aatagcagca tcaacaaagc cttcactacg aagtcgctgt
     1381 acttctgccc tgatcatgcg tgatggttca acccaaagca ccgatccgaa aattatgatt
     1441 aatgtgatga tagatggttg aaacaatgat gagagtgcaa tgatgataat aagttgtggt
     1501 acggctacca tgacttgtgt cagcaatgaa atccaaaaat caggattgag atggatatga
     1561 tttgttgctt tgatttttct tttattagat aacttccaaa ataatattaa agcaattgtt
     1621 aaaagcagaa atagaataaa ggaaggtgat aagtctatta aattgattaa gctaaattgc
     1681 tgaaacaggt tgtgccattc cattgagaat gtgtaaaagc cataaaacca gaccagaaac
     1741 agaaatatta ttagtgaaac aaattttatt ctatttaaat aaattccaga gtcagcaaaa
     1801 tatcctgaac agatacctgc aatcaatgaa attacaatac taaaaatcat tgaaaacaag
     1861 gcaataatta aactgtgtcg taaaccaaaa acaattcctg cagcaacgtc agcaccaatt
     1921 gcatctgtac ctaaccaatg atgaaaccgc agaggtaatt ctatttgttg tcctgtattg
     1981 tttgtataat aacttttttg aaacggactt aataacgcta tatcattttg tcttggctga
     2041 ccgggtgagt agggtatagg gcgcagaatt tgattattgc ctttgctctc aattccgaat
     2101 gataaatcat agtcagaagt tagattgtca tttccaaaaa atggtttttc attagcaata
     2161 aatggagcaa acaatgcaag gagtacaact gtcagcagcc aaagttttgc taaacgaaat
     2221 ttcaacttcc tcctcatggc tgaacagttt tagttcgtgg gtcaacataa tgctgaatcc
     2281 attcattaag cagataaagt aaaattgtaa ttagtcctgt aattaaaata acagcagaaa
     2341 tgaaaggata gtttcttgtc gtagtagcat gaatcagttc aagtcccata cccggaatag
     2401 aaaacaatga ttcaataatt accgaaccgc caataactgc tggaaacata tttgcaagca
     2461 aggctacaaa tggcgatatg atatttggaa aaatgtgttt gataacaact tctttttttg
     2521 ttagcccttt tgcttgcgcc attaaggcat aaggtttttt aaattcagct gaaattaggc
     2581 tggcagatgt acgttgaaca aatgcccaca gggaataggt gtagcaaatt agcggaagga
     2641 taagataggg aagggtgccg ataaattttt ctgaaaaact tgcgcttgaa gaatatcctc
     2701 caataggcat aactcctgaa gatggaaata tttgtaagaa atcaggatta gagaaaaaat
     2761 atagtaaggc tgttcctgca aaatagaccg gtagagaata aaaaagtacg gtaatcatat
     2821 tgataatgcg gctggcaatt ttgttttttg tatatgccaa ccatattgaa attaaaaggc
     2881 cggaaataaa tgctatgata attgccagaa cagtcagaag caatgaccag aatatttttt
     2941 ctttcagtaa tgagccaatg cttcttccgg tatagagtga ttgacctaag tttaaatgta
     3001 tgatgccact attcttgtct gttccaaaaa accagtcatg aaaacgattt tgtgcattaa
     3061 gttgaactgc aggaacccat tttttccatg atgatttttt agtttgcatt gcattatagg
     3121 aatgcaaaat agaattagcg agtgaattaa gtttaagtat agttgcataa ttgttgaaag
     3181 aatcaagaat actgtcattc ttgctcagtt ttacttgagt ttctatctga aataaactat
     3241 ttgctgcatt agaagcagtt tgcctgattg cagtatcagt tgctgaagcg aatgatgttg
     3301 taattgctgt tcttgcagca ttaattttct tgtaaaaaat aaatacttca ctaccacaac
     3361 caaaatttcg caacaaatga ttgtagcttt tttgttgttt ctctgaagga agatattgtt
     3421 gccatccata ttcagctaag gatattacag aaaaataaaa taaaggcttg tcatagccta
     3481 gacgttgt
//
LOCUS       DAOOOL010000204         3465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3151_length_3465_cov_63.158358, whole genome shotgun sequence.
ACCESSION   DAOOOL010000204 DAOOOL010000000
VERSION     DAOOOL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_3465_cov_63.158358"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>221
                     /locus_tag="PKU82_11500"
     CDS             <1..>221
                     /locus_tag="PKU82_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOZ83531.1"
                     /translation="PVIITVYTDKSFEFIIKRPPVSIQLMEAAKIKTGSAESNRKKAG
                     SVKWEQVKAIAEDKMPDLNCFTIESAMSMV"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            392..1090
                     /gene="rplA"
                     /locus_tag="PKU82_11505"
     CDS             392..1090
                     /gene="rplA"
                     /locus_tag="PKU82_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOZ83532.1"
                     /translation="MAKLTKNQKLVNAKYDSEKSYTLQDASKIVKDITFTKFDASVDL
                     SIRLGVDPRKANQMVRGIVTLPHGTGKTVKVLVLCTPDKEAEAKAAGADYVGLDEYIT
                     KIEQGWVDMDVVITMPAVMAKVGKLGRVLGPRNLMPNPKTGTVTTDVGKAVTEVKGGK
                     IDFKVDKTGIIHASVGKVSFEPNKLFENANELLQSIIKLKPSSAKGTYLRSVSMSSTM
                     SPGVKVDTKSIAGQ"
     gene            1113..1637
                     /gene="rplJ"
                     /locus_tag="PKU82_11510"
     CDS             1113..1637
                     /gene="rplJ"
                     /locus_tag="PKU82_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOZ83533.1"
                     /translation="MTREEKNIEIDSLAQQFSEFPNFYITNVESLNSDKTSKLRRLCY
                     SRNVKLRVAKNNLIKKALQKLDPTAYDTLIPALKGTSAIMFSEVSNVPAKLIKEFRAK
                     DSVPAIKAAWIDSSIFVGDNQLEVLANLKSKNELIAEVIALLQSPAKNVVSQLTSGGS
                     KLAGILKTLEEKGS"
     gene            1708..2085
                     /gene="rplL"
                     /locus_tag="PKU82_11515"
     CDS             1708..2085
                     /gene="rplL"
                     /locus_tag="PKU82_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOZ83534.1"
                     /translation="MADLNAFAEQLVNLTVKEVQELSTILKDKYGIEPAAAAPVMMAG
                     GGGGGAAAAEEKTSFDVILKSGGNAKLAVVKLVKELSGLGLKEAKDLVDGAPKPVKEG
                     VSKEEANSIKQQLEEAGAEVEIK"
     gene            2220..>3465
                     /gene="rpoB"
                     /locus_tag="PKU82_11520"
     CDS             2220..>3465
                     /gene="rpoB"
                     /locus_tag="PKU82_11520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665681.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOZ83535.1"
                     /translation="MTSTPDKGGSKRINFSKNKNLIDYPDFLDIQLKSFQDFFQIETT
                     SDNRQNEGLYKVFAENFPITDSRNNFVLEFLDYFIDPPRYSIQECLERGLTHSVPLKA
                     KLKLYCTDPEHEDFETIVQDVYLGTIPYMTPKGTFVINGAERVIVSQLHRSPGVFFGQ
                     SRHANGTKLYSARVIPFKGSWIEFATDINSVMYAYIDRKKKFPVTTLLRAIGFESDKQ
                     ILEIFGLADEIKVSKSGLKSHVGRKLAARVLKSWVEDFVDEDTGEVVSIERNEIILER
                     ETFLEDHHIDLIIEAGVKSIILHKEDGNSADYAIIYNTLQKDTSNSEKEAVEHIYRQL
                     RNAEPPDEETARGIIEKLFFSDKRYDLGEVGRYRINKKLNLNTPEDVKVLTKEDIILI
                     IKYLIQLINSKADVDDIDHLSNR"
BASE COUNT         1196 a          601 c          709 g          949 t
ORIGIN      
        1 cctgtaatta ttactgtata taccgataag tcatttgaat ttataatcaa acgtccaccg
       61 gtatcaatac aattgatgga agcagccaag attaaaactg ggtctgctga atcgaatcgt
      121 aaaaaagctg gatcggttaa gtgggaacaa gtaaaagcta ttgctgaaga taaaatgcct
      181 gacttaaatt gctttaccat tgaatctgct atgagcatgg tnnnnnnnnn ntgcaggtac
      241 tgcacgtagt cttggtatta caattgaagg gcaaaagcct tattaattac tgccggacaa
      301 caaaggttgt tcagcatgat ttgaaaaagt aaattaacag aaggagtttc ggtttataaa
      361 cggaacgtta tcacttcaca aattatataa aatggcaaaa ctgacaaaaa accagaagtt
      421 ggttaatgcg aagtacgaca gtgaaaaatc atatacactg caggatgctt ctaagattgt
      481 taaagacatc acgtttacaa agtttgatgc atcagttgat ctgagcatta gattaggcgt
      541 tgatccacgt aaagcaaatc agatggttcg tggcatcgtt accctgccgc atggaacagg
      601 aaagactgta aaagtattgg tgctttgcac acccgacaaa gaagcagaag caaaagctgc
      661 cggtgccgat tatgttggat tggatgagta tatcaccaaa atcgaacaag gttgggttga
      721 tatggatgtg gtaattacca tgcctgcagt aatggctaag gtaggtaaat taggtcgtgt
      781 attaggtcct cgtaacctta tgccaaatcc aaagacagga actgttacta ctgatgtagg
      841 taaagctgtg actgaagtta agggcggtaa gattgatttt aaagttgaca aaacaggaat
      901 cattcatgca tcagttggta aagtatcatt tgagccgaac aaactttttg aaaacgccaa
      961 tgagttgctt cagagtatta tcaaattaaa accatcatca gcaaaaggta cttatctgag
     1021 aagtgtttca atgagcagta caatgagtcc cggtgttaag gtggatacaa aatcaattgc
     1081 aggtcaataa tcatatataa attattcaac aaatgacaag agaagaaaaa aatatagaaa
     1141 tagatagcct ggcacaacag ttcagcgagt ttccaaactt ctacattact aatgtagaga
     1201 gcctgaacag tgataaaaca tctaagctgc gcagactttg ttacagcaga aatgtaaaac
     1261 tgcgtgtagc aaaaaataac ctcatcaaga aggcattaca aaagttggat ccaactgctt
     1321 atgatacgtt gattcctgca ttaaaaggta cttcggcaat tatgttcagt gaagtatcaa
     1381 acgtgcctgc aaaacttata aaagagttca gagcaaaaga cagcgttcct gcaataaaag
     1441 ctgcatggat tgattcatca atctttgttg gcgacaatca gttagaggta ttagcaaatc
     1501 tgaaatctaa gaatgaactt attgcagagg ttattgcatt acttcagtca cctgctaaaa
     1561 atgtggtttc acaacttaca tctggcggca gcaaactggc aggtatatta aaaacattag
     1621 aagagaaagg ttcttaagct tccacctttg aatcttaaac tatagtacaa ttcaaataaa
     1681 catttaaaac aattaaaatt aaataaaatg gcagatttaa atgcattcgc agaacagttg
     1741 gtaaacctga cagtaaaaga agttcaggaa ctttcaacca ttctaaaaga caaatacggt
     1801 attgagcctg ctgctgcagc accagttatg atggccggtg gcggtggcgg tggcgcagct
     1861 gctgctgaag aaaaaacatc ttttgatgta attcttaagt caggtggtaa tgcaaaactg
     1921 gcagtagtaa agctggtaaa agaattgagc ggacttggct tgaaagaagc taaagactta
     1981 gttgatggcg ctccaaaacc ggttaaagaa ggtgtttcta aggaagaagc aaattctatc
     2041 aagcaacaac ttgaagaagc cggagctgaa gttgagatca agtaatacct cggtttaaaa
     2101 aatcataggc cttttccaaa tggggaaggc ctatacccct tttacagcct ctgcaattgc
     2161 acaggctttt agtacaacaa ggattgaaat caatataaaa acaaatacac ttggctgcaa
     2221 tcacatcaac gccagataaa ggcggttcaa aaagaataaa cttctctaag aataaaaact
     2281 taattgatta tcctgacttt ctcgacattc agttgaaatc gtttcaggat tttttccaga
     2341 tcgaaacaac atctgacaac cgtcagaatg aaggtcttta taaagtattc gcagaaaact
     2401 ttccgataac ggattcacga aataattttg tgcttgagtt tctcgattat tttattgacc
     2461 cgccaagata ttctattcag gaatgtcttg aaagaggact cacacacagt gtacctctaa
     2521 aagctaaatt aaaattgtac tgtaccgacc ctgagcatga ggatttcgaa acaattgttc
     2581 aggatgttta cttaggtaca attccgtaca tgacaccaaa gggaaccttt gtgattaatg
     2641 gtgcagaacg cgttattgtt tcgcagttgc accgttcacc gggagtattt ttcggacaaa
     2701 gccgtcatgc taacggaaca aaactttatt cagcacgtgt aattcctttc aaaggatcgt
     2761 ggattgaatt tgcaacagac attaacagtg tcatgtatgc gtacattgac cgtaaaaaga
     2821 agtttccggt taccacattg ttgcgtgcaa taggttttga aagcgataaa cagattcttg
     2881 aaattttcgg acttgctgac gagattaaag taagcaaatc aggattaaaa tcacatgttg
     2941 gccgtaagtt agctgcacgt gttcttaaat cgtgggttga agattttgtg gatgaagata
     3001 ccggtgaagt agtttctatc gagcgtaatg aaattattct tgagcgtgaa accttccttg
     3061 aagatcatca cattgacctg attattgaag ccggagtaaa atccattatc cttcataaag
     3121 aagatggcaa ctctgctgac tatgcaatta tttacaacac gctgcagaaa gatacttcca
     3181 actctgaaaa agaagcggtt gaacatatct atcgtcagtt gcgtaatgca gaaccaccgg
     3241 atgaggaaac agcacgtggt atcatcgaaa aacttttctt ctctgataag agatatgatt
     3301 taggtgaagt aggtcgttac cgtatcaata aaaaacttaa cctcaataca cctgaagatg
     3361 tgaaggtgct tactaaagag gatattattt taatcataaa gtacctcatt cagcttatca
     3421 actctaaagc tgatgtggat gatattgatc acttatcaaa cagac
//
LOCUS       DAOOOL010000205         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3173_length_3456_cov_114.491914, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000205 DAOOOL010000000
VERSION     DAOOOL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_3456_cov_114.491914"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1144)
                     /gene="pheS"
                     /locus_tag="PKU82_11525"
     CDS             complement(122..1144)
                     /gene="pheS"
                     /locus_tag="PKU82_11525"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOZ83536.1"
                     /translation="MSQELIEQHIQEAETFSIQSPADLENFRLKYLSKKGVLADMFAL
                     MKNIEPAERKNYGLQVNLLKQKTEAIFESFKEKFENVTTDEKKIDITLPGEPMSEGAL
                     HPLSIIRNRIIEIFSAVGFTVSDGPEIEDDWHNFTALNFPEEHPARDMQDTFFIRKNP
                     DLALRTHTSSVQVRIMEKHKPPLRTISPGRVYRNEAISARAHCFFHQVEGLYIDKDVS
                     FADLKQTLLYFAREMFGADTEIRLRPSYFPFTEPSAEMDISCNFCKGEGCNICKYTGW
                     VEILGCGMIDPAVLDNCKIDSNVYSGFAFGMGIERITMLVYNINDLRIFSENDVRFLK
                     QFSGIG"
     gene            complement(1237..>3456)
                     /locus_tag="PKU82_11530"
     CDS             complement(1237..>3456)
                     /locus_tag="PKU82_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOZ83537.1"
                     /translation="PDEAGMPLIRAYRGLPKNRALVKFLSEQGVRALLQKTENHYMQD
                     QSKEMHKVDAELFFVIDEKNNTVELTEKGIELITANNEDTHFFVMPDVGASIAEIEKS
                     NGTNEEKLERKEALMRDFSVKSERIHTINQLLKAYTLFEKDDEYIIADGKVKIVDEQT
                     GRVLEGRRYSDGLHQAIEAKEGVKIEAATQTYATVTLQNYFRMYHKLAGMTGTAETEA
                     GEFWQIYKLDVVVIPTNKKIIRDDREDLVYKTKREKYNAVIEEIAKLTAAGRPVLVGT
                     TSVEISELLSRMLKQRNIKHNVLNAKLHKKEADIVAEAGQAGTVTIATNMAGRGTDIK
                     LGQGVKEAGGLAIIGTERHDSRRVDRQLRGRSGRQGDPGSSQFFVSLEDDLMRLFGSE
                     RIAKLMDRMGIEEGEVIQHSMITKSIERAQKKVEENNFGIRKRLLEYDDVMNAQREVI
                     YKKRRHALFGERLALDIMNMQYDVCESIISSYNQERDFDALQLDLIRYLSIEAPIDAK
                     AFLQIKLEDLTQMVFEKANGHYRQKSNLIAEKAFPVIENIYTNQGQTIVDVVVPFSDG
                     TKAMNVIAGLKKSYDSKCKELISALEKSSVLNFIDEAWTEHLREMDDLRQSVQNAVYE
                     QKDPLLIYKFESFELFKQMIDRVNKEIVSFLIKSYIPVQNQENVREAKVQQQRTDMSK
                     MQTSHNESDAGGQSRPQGPPPPPKQQPAKAEIKVGRNDPCPCGSGKKFKNCHGAGVD"
BASE COUNT          954 a          760 c          594 g         1148 t
ORIGIN      
        1 tactaaaagg tattgagaaa ccaataaaca aaattgtatg agccaacgtc ccgatagttt
       61 tcgggacaac actaagtatt gtagttggga caggaataag aaagcataaa gtaaaccacc
      121 cctaccctat cccactgaac tgctttaaaa aacgaacatc attttcagaa aaaattcgca
      181 ggtcgttgat gttataaacc aacatagtaa ttctttctat acccataccg aaggcaaaac
      241 cgctgtaaac attgctgtct attttacaat tgtcgagaac ggcaggatca atcatgccac
      301 agccaagaat ttctacccag cctgtgtatt tacagatgtt gcagccttcg cccttacaga
      361 aattacagga tatatccatc tctgctgatg gctctgtaaa cggaaaatac gaagggcgta
      421 aacgaatttc agtgtcagca ccaaacatct ctcttgcaaa atagagtaat gtttgtttca
      481 ggtcggcaaa cgaaacatct ttgtcaatgt agagtccttc tacctgatga aaaaaacaat
      541 gtgcccttgc tgaaatcgct tcattacggt aaactcttcc cggtgatatt gtacgtaatg
      601 ggggtttgtg tttttccatt attctcacct gtacagatga tgtatgtgta cgcagtgcaa
      661 ggtcagggtt tttgcggata aaaaaagtat cctgcatgtc gcgggccgga tgctcttcag
      721 gaaaattgag tgctgtaaaa ttatgccagt catcttctat ttcaggtcca tcagaaactg
      781 tgaatccaac agcactgaaa atttcgataa ttctgtttct gataattgat agcggatgca
      841 atgcaccttc actcattggc tcaccgggca aggtaatgtc tatcttcttt tcgtcagtag
      901 taacgttctc aaatttctct ttaaaacttt cgaagatggc ttctgtcttt tgtttgagca
      961 agttaacctg caacccgtaa ttttttcttt cggcaggttc aatatttttc atcaatgcaa
     1021 acatatctgc caacacacct ttcttagaaa gatatttaag acggaaattt tcaagatcgg
     1081 caggtgactg aattgaaaat gtctctgcct cctgaatatg ctgctcaatt aattcttgcg
     1141 acataagata aaataagata atgcaaatta acgattaaaa cttttgctta tgatgttaat
     1201 attctattag aaataatgac aaataaaact attgtattaa tcaacacctg caccatgaca
     1261 gtttttaaat ttctttccac taccacaagg gcaagggtca ttacgtccca cctttatttc
     1321 tgcctttgca ggttgttgct ttggtggtgg cggtggtcct tgcggacggc tttgccctcc
     1381 tgcatctgat tcgttgtggc ttgtttgcat tttgctcata tcagtacgtt gctgctgcac
     1441 tttagcttca cgtacatttt cctgattttg aacagggata tagcttttaa tcaaaaacga
     1501 aacaatttct ttattcaccc tgtcaatcat ctgcttgaaa agttcaaacg actcaaactt
     1561 atatatcaac aaaggatctt tctgttcgta aactgcattt tgtacactct gacgcaagtc
     1621 gtccatttcg cgcaaatgct ctgtccatgc ttcgtcaata aaattgagta ccgatgattt
     1681 ttcgagagca gaaatcaact ctttacattt tgaatcgtac gacttcttta gtcctgcaat
     1741 tacattcatt gccttagtac catcactaaa tggcacaaca acatctacaa ttgtctgacc
     1801 ctgattagtg taaatatttt caataacagg aaatgctttt tctgcaataa gatttgattt
     1861 ctgtcggtaa tggccgtttg ctttttcgaa aaccatttgt gtcaggtctt cgagttttat
     1921 ctgcaaaaat gcttttgcat ctatgggtgc ttcaattgac agataacgaa taaggtctaa
     1981 ctgtaatgcg tcaaaatcgc gctcctgatt atagctgctt ataatgcttt cgcaaacatc
     2041 atattgcatg ttcataatgt cgagtgcaag cctttctcca aacaatgcat gacgtctttt
     2101 cttatagata acttcgcgct gtgcattcat cacatcatcg tattcgagca aacgtttacg
     2161 tattccgaag ttattctctt caaccttctt ctgtgcacgt tcaatagatt tggtaatcat
     2221 gctatgctga ataacctcac cctcttcaat gcccattctg tccattagtt tggcaattct
     2281 ttcactgccg aacaaacgca ttaaatcatc ttctaacgaa acaaaaaact gtgatgaacc
     2341 cgggtcaccc tgacgacctg aacgtccgcg caactgtctg tcaacacgtc ttgagtcatg
     2401 gcgttctgta ccgataattg caagaccacc ggcttctttt actccctgac ctaatttaat
     2461 atctgtacca cgaccggcca tgttggtagc aatagttacc gttccagcct gccctgcttc
     2521 tgcaacaata tccgcttctt tcttatgtaa cttagcattt aaaacattgt gcttaatatt
     2581 tctttgcttg agcatacggc taagcaattc agaaatttca accgatgttg ttcctacaag
     2641 aaccggtctt cctgcagctg ttagttttgc aatctcttca ataacggcat tgtatttttc
     2701 acgcttggtt ttataaacca aatcttctct gtcgtcacga ataatttttt tgttggtagg
     2761 aataactact acatccaact tatatatctg ccaaaattct ccggcttctg tttctgcagt
     2821 acctgtcata ccagccagtt tgtggtacat acggaaatag ttctgcaagg tcaccgttgc
     2881 atacgtttgt gtagcagctt caatctttac accttcttta gcttcaattg cctgatgtaa
     2941 accatcggaa tatcttcttc cttcaagaac acgtcctgtt tgctcatcaa caattttcac
     3001 ttttccatct gcgataatat actcatcatc tttttcgaaa agcgtatatg cttttagtaa
     3061 ctgattgatg gtgtgaatac gctctgattt tacagagaag tcgcgcatca gggcttcttt
     3121 gcgttctaac ttttcttcgt tagttccatt agatttttca atttctgcaa tagaagcacc
     3181 aacatcaggc atcacaaaaa agtgtgtgtc ttcgttgttg gcagtaatca gttcaatacc
     3241 tttctctgta agttcaacgg tattattttt ttcgtcaatc acaaaaaaca attctgcatc
     3301 tactttatgc atttcctttg actgatcctg catgtagtgg ttttctgttt tctgcaataa
     3361 tgctcttaca ccctgctcac ttaaaaactt taccaatgct ctgtttttgg gcagacctct
     3421 gtaagctctt atcaagggca tgcctgcttc atcggg
//
LOCUS       DAOOOL010000206         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3175_length_3455_cov_107.518235, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000206 DAOOOL010000000
VERSION     DAOOOL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_3455_cov_107.518235"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKU82_11535"
     CDS             <1..201
                     /locus_tag="PKU82_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOZ83538.1"
                     /translation="PILVIHNEKDFRVPLSQGMEAFSGARLQNIPARFLYFPDEGHWV
                     NKPQNSVMWQRVFFDWLDRYLK"
     gene            complement(321..2135)
                     /locus_tag="PKU82_11540"
     CDS             complement(321..2135)
                     /locus_tag="PKU82_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83539.1"
                     /translation="MKTNIYYLRKVVIALLAIILMSYSTQATHLFGGEITWKCLGTGK
                     YQFTLKIYRDCMGFPFSPPAALDVYGNPNITSIPINPAYTVYNDLTPTSCGFDCNNPQ
                     PGAVIECIMKTDPIFLVGIPPPATGWVIAYDDCCRQPLNNLGGGGQGFTIRATIYSYN
                     NLSSAPCFDSSPFFAERPYAFTCIGYPFTYNPNAIDADGDSLVYEWAYCLEQPTFTPP
                     AAFNAPSITISFNPPPYTRLNQIPGNPVLDSHSGELSFFVDTASATIGSFGSCIKVSA
                     YRCGKKIAEIFRDCAFVLTNNCYVQPGPPAINLPPVYNLPFNSGTASDTVVTAGDTVR
                     FSLHFTDIQQNMGAFPTQFITINASGSYFGTNFTIDTAGCPSPPCATLNKTLPSSSPI
                     VNSVNFQWVPNCTLINLNTCNNNPENTYYFVFDASDNFCPVPAHAYVTAKITVAGPSI
                     IQSNDTLYVNYPGAISYQWYNGGNLIPGATNSFYIVTVPGLYSCQISNTTGCNISSRL
                     MQVTQTSIAETENLFSWDIIPNPASDKVTIAINSPVANEGKIVLTDISGRIIEQQQIQ
                     LLKGRNTFNINTHSFARGMYQLCLQSGKTKLMEKLLLK"
     gene            complement(2253..>3455)
                     /gene="rseP"
                     /locus_tag="PKU82_11545"
     CDS             complement(2253..>3455)
                     /gene="rseP"
                     /locus_tag="PKU82_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOZ83540.1"
                     /translation="DAWGIKLFSFKKGDCEYGIGWLPFGGYVKIAGMVDESMDKDQLA
                     QPAQPWEFRSKPAWQRLIVMVAGVVMNVILGIAIFSMSLLHYKGSYLKNDAVTHGIVA
                     YKLGEEIGLRTGDKVVAIDGKSFDRFDDLLSTRVLFGATLTVIRNGEKVEIEVPDDFY
                     KKTGPNARSLFLGAGHLTYVVKEVVVGENAFKAGVENDDIITEVDKQKITGEDFMQRY
                     FADKKGETALLSIIRAGKPMDITVNISSKGTIGVRYNGDVVVPADYVKTPYTLASAFA
                     FGTSDAFETLVSNAKGFKKLFKGEEKFQESVQGPIGIAKIYGGTWDWPHFWVLTGLLS
                     MILAFMNILPIPALDGGHVLFLLIESVTRRKFSDAFMEKTQVVGMVLLLSLMVFVIGN
                     DIWRAFIK"
BASE COUNT         1118 a          653 c          625 g         1059 t
ORIGIN      
        1 ccaattcttg ttattcataa cgaaaaagat ttcagagtac cgttgagtca gggcatggaa
       61 gcattttccg gagcacgact acaaaatatt cctgcacgat ttttatattt ccctgacgaa
      121 ggacattggg ttaacaaacc acaaaacagt gttatgtggc agcgtgtatt tttcgattgg
      181 ttggatagat atttgaagta ataaaaaagc gggacaagcc cgcttttttg ttttgcattg
      241 gagagtttag aaatctttat agctatatat tttgtcttac attattaatc aactcctttt
      301 cagattcaat caactcaact tcatttcaac agtaattttt ccatcaactt tgtttttccg
      361 gattgcaagc acaactgata catacctctt gcaaaagaat gtgtgttgat gttgaaagta
      421 tttcggcctt ttaaaagttg aatctgttgc tgctctatta tacgaccgga aatatcggta
      481 agcacaattt taccctcatt agctaccggt gagttaatag caatagtaac tttatcagaa
      541 gccggattgg gaataatgtc ccaggaaaat aaattttctg tttcagcaat agatgtttgt
      601 gttacctgca ttaatcttga actgatatta caaccagttg tgttacttat ttgacatgaa
      661 tacaagcccg gaaccgtaac tatataaaaa gaattggttg ctcccggaat gagattacca
      721 ccattatacc attgatatga aatagcaccc ggatagttta catacagtgt atcgtttgac
      781 tgtataattg atgggcctgc aacagtaatt ttagccgtta cataggcatg agcaggtaca
      841 ggacaaaagt tatctgatgc atcaaaaaca aaatagtatg tattttccgg attgttattg
      901 catgtattga ggttgattaa tgtacaattc ggcacccatt gaaaattaac ggaatttact
      961 attggtgatg atgatggtaa agtcttgttt agtgttgcac atggtggaga tggacaacct
     1021 gcagtatcaa tagtaaagtt ggtaccaaaa tatgaaccgc ttgcattgat ggttataaat
     1081 tgagtaggaa aagcacccat gttttgttga atatccgtaa aatgcaacga gaatctaaca
     1141 gtgtcgcctg cagttacaac tgtatcagaa gcagtacctg aattaaacgg taagttatat
     1201 acaggcggca gatttattgc aggaggcccc ggttgtacat agcagttgtt agttaagaca
     1261 aaggcacagt ctctaaaaat ttcagcaatt ttttttccgc agcgataagc agaaactttt
     1321 atacaactcc caaaacttcc tattgttgca cttgcagtgt caacaaaaaa actcaattcg
     1381 cctgaatgac tatctaatac tgggttgccg ggaatttgat ttaatcgtgt gtatggtgga
     1441 ggattaaatg aaatggtaat actaggagca ttaaaggctg caggtggagt aaaggtgggt
     1501 tgttctaaac agtaagccca ttcgtaaacc aaagaatctc catctgcgtc aatggcattt
     1561 ggattataag taaatgggta accaatacaa gtaaaagcat aaggtcgttc tgcaaaaaaa
     1621 ggtgatgaat caaagcatgg cgcagaactc aaattgttat aagaataaat ggtggcacgt
     1681 atcgtaaaac cttgtccacc accacctaaa ttatttaatg gttgtctgca gcaatcatca
     1741 taagcaatca cccatcctgt agcaggaggg ggtattccaa caagaaaaat cgggtcggtt
     1801 ttcattatac attcaattac agcacctggt tgtggattgt tgcagtcaaa accacaactt
     1861 gttggtgtta aatcgttata aaccgtatat gcagggttaa taggtatgga agtaatatta
     1921 gggtttccat aaacatctaa tgctgcagga ggactaaatg gaaatcccat acaatcgcga
     1981 tatattttga gtgtaaattg atatttaccg gtcccaagac atttccatgt gatttcccca
     2041 ccgaataaat gcgttgcctg tgttgaataa ctcatcaaga ttatagctaa taaagctata
     2101 acaactttcc gaaggtaata gatgtttgtt ttcatgatac atatatttgt tagtacaaat
     2161 ataacagaat ttaattaaaa aacaatttaa ttaaatattt ctttcaaaat agcagaaaga
     2221 tagctcaatg gtagaatttt aatttcagaa aactacttaa taaaagcccg ccagatatcg
     2281 tttccgatta cgaagaccat taatgagagg agtaacacca ttccaacaac ctgagttttt
     2341 tccataaagg catcactgaa ttttctgcgt gtgacagatt caatgagcag aaataaaaca
     2401 tgcccaccat cgagtgcagg aattggaagt atattcataa aagcaagaat catagagagt
     2461 aatccggtaa gtacccaaaa atgtggccag tcccaggtgc cgccatatat ttttgcaata
     2521 ccaataggcc cctgaacaga ttcctgaaat ttttcttctc ctttaaaaag tttcttaaag
     2581 ccttttgcat tactcactaa tgtttcaaat gcatctgagg taccaaatgc aaatgccgat
     2641 gccaatgtgt aaggagtttt aacataatcc gcaggaacaa caacatcacc attatatcgt
     2701 acgccaattg ttcctttaga acttatgttt accgtaatgt ccattggttt accggcacga
     2761 ataattgaga gcaatgcagt ttcacctttt ttatcggcaa agtaacgctg cataaaatct
     2821 tcaccggtaa ttttttgctt atcaacttct gtaatgatgt catcgttttc tactcctgct
     2881 ttaaaagcat tttcgccaac aacaacttct ttaacaacat aggtcagatg tcctgctcca
     2941 agaaaaaggg aacgtgcatt aggtcctgtt tttttataaa aatcgtctgg cacttcaatt
     3001 tctacttttt caccatttct tataacagtt aatgtagcac caaacaatac gcgtgttgaa
     3061 agcaggtcat caaaacgatc gaaagattta ccatcaatag caacaacttt atcacctgtg
     3121 cgcaagccaa tttcttctcc cagcttataa gcaactatgc catgtgtaac agcatcgttt
     3181 ttcagatagc tgcctttgta gtgcaacaaa ctcattgaaa aaattgctat accaagaatg
     3241 acattcatta caacacctgc aaccattaca attaatcttt gccatgcagg tttacttctg
     3301 aattcccatg gttgtgccgg ctgtgctaat tgatctttat ccatgctttc atcaaccata
     3361 cctgcaattt ttacatagcc gccaaaagga agccatccta taccatactc acagtcgcct
     3421 tttttaaagc taaacaattt tattccccat gcatc
//
LOCUS       DAOOOL010000207         3449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3184_length_3449_cov_60.937537, whole genome shotgun sequence.
ACCESSION   DAOOOL010000207 DAOOOL010000000
VERSION     DAOOOL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3184_length_3449_cov_60.937537"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2743
                     /locus_tag="PKU82_11550"
     CDS             <1..2743
                     /locus_tag="PKU82_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ83541.1"
                     /translation="VVPTTICAYTPVQFNVLNPDTNNIYHFSGDGGMLSHCYNQTNPI
                     WSFNTQTGQQTITLSTSNNGCISTATQTVTVKGPMAKFYVAGNCNTPFKYSFPATIND
                     ATTWNWDFGDGTALINSGNVNPTHTYAATGDYWVKLTSFNTTSGCAPDIDSNIVRVRN
                     ISAEFTRDSVACKSYTINVDATNATDAYGSCNEGFLWYWGDGTFPHNSSSPIAAHTYF
                     NSGVFYVKLVTTDINGCVDSVKKKVKVYESNAFFKTNKNYGCLPLTVNFTDSSYADTT
                     IASYSWDFGDGSTSSVKNPSHTYTTAPAGGSWTVTLIVTDTIGCTDVYQKTITASLPD
                     TLFSATKTNICVGEPIVFNPHFTGHSSYSWSFGDNTTSAQVSPSHSYNAAGAYTVTLS
                     VTDSIGCPGSKKITNYINVQAYPKAGFTSSADSMLNKCYPLLVNFYDTSIVSVFGSRK
                     WNLGNSSNIFTTPVAGTIYQTPGIYYIQLIEETTFGCRDTAYDTLRVLGPVGNFNIAP
                     TTICKGQSITFSIKDTADLGAFSWDFGDGMTSPGISPITHTYNINPVSGQTLVGLVMW
                     SPDSACTYTQTNPVFIRKVIANFNINSGDTALCIGEPVTLVNTSLNASNNYWNFGNGQ
                     TFNGATPPPYTINQTGVYTITLAIDNSAWGCKDTIRKKISINNLPTVNAIGGDTCAGK
                     PITLHATGGVSYLWSPATGLSNTTVANPVATPGQSTVYTVLITDVNGCTGVATAPVTI
                     YNPPPQVNVTHLLFVGDSVQLNTGLTNNLIINWNPINGLSCVDCPNPWAQPLVNTLYT
                     ATFTDSAGCFSGISRFNIEIDPRITIDVPTAFTPNGDGDNDVIFVNGLGLKRLIEFKI
                     YNRWGQLIFESNDLNKGWDGYFKGQLQNVETYVYTAKAEGWLNGKIVSKKGTFNLLR"
     gene            2792..3031
                     /locus_tag="PKU82_11555"
                     /pseudo
     CDS             2792..3031
                     /locus_tag="PKU82_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            3041..>3449
                     /locus_tag="PKU82_11560"
     CDS             3041..>3449
                     /locus_tag="PKU82_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HOZ83542.1"
                     /translation="MKISIRFFNDREVRAVWDDANAKWWFSVLDIVGVLNNESDYSKV
                     RNYWKYLKAKLKKENNQLVSAATQFKFLAPDGKKRMADMLDSDGVTALAKNFPNNRAM
                     KFLDWFLYSDTSIDGQSKKKAYTLFESNLINEFE"
BASE COUNT         1108 a          680 c          665 g          996 t
ORIGIN      
        1 tgttgttcct acaactatat gtgcatacac acctgtacag tttaatgttc tgaatcctga
       61 caccaataat atttatcatt tttctggtga tggaggaatg ctgtcgcatt gttacaatca
      121 aaccaatcct atctggagtt tcaacacaca aacagggcaa cagacaatta cactttctac
      181 atcaaacaat ggttgcatca gcacagctac acaaactgtc actgttaaag gacctatggc
      241 gaaattttat gtggccggaa attgtaatac tccatttaaa tatagttttc ctgcaacgat
      301 taacgatgct accacctgga actgggattt tggtgatggc acagcattaa ttaattcagg
      361 aaacgtaaat ccaacacata cctatgctgc tacaggcgac tattgggtaa aattaacttc
      421 attcaacaca acatctggtt gtgcacctga tattgattcc aatattgttc gcgtcagaaa
      481 tatatcggca gaatttacca gagattctgt tgcctgtaaa agctatacta taaatgtaga
      541 tgccacgaac gctactgatg cttatggcag ttgcaatgaa ggttttttat ggtattgggg
      601 cgatggaacc tttcctcata attcttcttc accaattgct gcccacactt attttaacag
      661 cggtgtgttt tatgtaaaac ttgtcaccac tgacattaat ggatgcgtgg attcagtaaa
      721 gaaaaaagtg aaagtttatg aatccaatgc tttttttaaa accaataaaa attatggctg
      781 tctgccacta acggtgaatt ttactgacag tagttatgcc gatacaacca tagcttctta
      841 ctcatgggac tttggtgatg gaagcacttc ttctgtcaaa aacccaagtc acacctacac
      901 tactgcacct gcaggtggaa gttggacagt aacattaatt gttactgata ctattggctg
      961 caccgatgtt taccaaaaga caattactgc gtcattgccg gatacgttat tctctgcaac
     1021 aaaaacaaat atttgcgttg gcgaacctat tgtcttcaat ccacatttta cgggacattc
     1081 atcatactcg tggagctttg gtgacaacac cacatcagca caggtatcac catcgcatag
     1141 ttataatgca gcaggtgcct acacagtaac attatctgtt accgattcca tcggctgtcc
     1201 tggaagtaaa aaaattacta actacatcaa tgtacaagcc tatcccaaag caggatttac
     1261 cagtagtgca gacagtatgc tcaataaatg ttatccattg ttggttaact tttatgatac
     1321 aagtattgtt agtgtttttg gcagccgcaa gtggaatctt ggcaacagca gtaacatttt
     1381 tacaacacct gtggcaggaa caatttatca gacgcccggc atttactaca ttcaacttat
     1441 tgaagagaca acctttggtt gcagagacac tgcttatgat acacttcgtg tacttggacc
     1501 tgttggcaac ttcaacattg caccaacaac aatttgtaaa gggcaatcaa ttactttcag
     1561 cattaaagat acagcagacc ttggtgcatt ttcgtgggac tttggtgatg gtatgacctc
     1621 tccgggaatt tcacccataa cacatacata caatattaat cccgtaagcg gacaaacgct
     1681 tgtaggtttg gtaatgtggt cgcctgactc ggcttgtact tacacacaaa ccaatccggt
     1741 gtttattcga aaagttattg caaacttcaa cattaacagt ggcgacactg ctttatgtat
     1801 tggtgaacct gttacattag tcaatacttc tttaaatgca agtaataact actggaactt
     1861 tggtaatggt cagactttta atggtgcaac accaccgcct tacaccatta atcaaacagg
     1921 ggtttacacc attacattag caatagataa ttcagcgtgg ggatgtaagg acactatcag
     1981 aaaaaaaata agcataaata atcttccgac agtaaatgca ataggtggcg acacttgtgc
     2041 aggcaaacca ataactttac atgcaacagg tggtgtaagt tatctgtggt cgccagcaac
     2101 aggactttca aacactacag ttgccaatcc tgttgccaca ccgggtcaaa gcaccgttta
     2161 taccgtattg ataaccgatg taaacggatg tacaggagtt gccactgcac ctgtaacaat
     2221 ttacaatcca cctccgcaag tcaatgtaac gcatttactt tttgttggcg actctgttca
     2281 attaaataca ggccttacaa ataatttaat catcaattgg aatcctatta acggattaag
     2341 ttgtgttgat tgccctaacc catgggcaca gccgttggta aacacacttt acactgcaac
     2401 atttacagat tctgcaggtt gcttttcagg catttcaaga ttcaacatag aaatagatcc
     2461 aagaataact attgatgttc caacagcttt tacacccaac ggtgatggcg ataacgatgt
     2521 aatatttgta aatggactcg gactgaaacg tctgatagaa tttaaaattt acaatcgttg
     2581 gggacagttg atttttgaaa gcaacgactt aaacaaaggc tgggatggct attttaaagg
     2641 tcagttgcag aatgtagaga cctatgttta cactgccaaa gcagaaggtt ggttgaatgg
     2701 caaaattgtg agtaagaaag gaactttcaa tttgttgaga tgatgtaaac cataataaga
     2761 tatcacatag gaacaaaatt ttagaaaaag caagagggat ttgtttgttg aagagaatat
     2821 tatggtagag ttgaaagccc tgataaaact tgaagaagtg catttggcac atgcaatgaa
     2881 ttattaccag gcctataatt tgcccatagg cttacttatc aattttggtg caaagagcct
     2941 tgaatttaaa agggtgtata atgttaatca tcctgagaat aagggctaca tcaaacgcaa
     3001 tcctacaatc atcaaatcca acaaatctta attcagacaa atgaaaatat ccatccgctt
     3061 cttcaacgac cgtgaggtgc gggctgtttg ggatgatgcc aacgccaagt ggtggttttc
     3121 ggtattggat attgtgggtg tattaaacaa tgaaagtgat tacagtaaag tccgtaacta
     3181 ttggaaatac cttaaagcca agttgaagaa agaaaacaat caactggtta gtgccgctac
     3241 ccagttcaaa tttcttgcac ccgatggcaa aaagagaatg gctgatatgt tggatagtga
     3301 tggcgtaaca gctctcgcca aaaattttcc aaacaacaga gccatgaagt ttttagactg
     3361 gtttctttat agcgatacca gcattgacgg acaaagcaag aaaaaagcct acaccctttt
     3421 tgaaagtaat ttaatcaacg aatttgaag
//
LOCUS       DAOOOL010000208         3446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3191_length_3446_cov_42.744913, whole genome shotgun sequence.
ACCESSION   DAOOOL010000208 DAOOOL010000000
VERSION     DAOOOL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_3446_cov_42.744913"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PKU82_11565"
     CDS             <1..1466
                     /locus_tag="PKU82_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83543.1"
                     /translation="GCGFYQVTIAAGDTICGFNFGVSLNSSSIEGYVFVDSNTNGIKD
                     AGEAGIAYQPVQIGNLTSYTDLTGKYERYVPIGTYTVSYTPTGVYASYPLTTASSNSV
                     SVVGVGITYQGGNYGLDLPAGSTNLAVNLLPHTTVTPGFPAWYDIQVCNIGINPTGAT
                     VTMNYDPGLTYTFGSPVPTSVNTTAHVITWNLPTMTSGQCLYIQADFSASTSYQIGDP
                     TFEFCSAYPTSGTDIDLTNNTDTVHQIVTGSWDPNNKLSVQTNNNNPVQQIVSSINSN
                     QEIEYTINFQNLGTAPAVNVVVVDNLSTDLVASSYQLTGASHNVSVSRNGMNVIYNFQ
                     NIMLPDAISNEPMSHGFVSYKINAVNGLAAGTQIADFSRIYFDFNSPVTTNIAVVTMV
                     NPTGINEQSEANLQSVYPNPVSDVATVQFTLGQNSLVGLELFDAAGRSTTLMPEVMMQ
                     AGLHKTNLNANQLSEGLYLLKLTVNGKSSVMKVTIKH"
     gene            complement(1556..3097)
                     /locus_tag="PKU82_11570"
     CDS             complement(1556..3097)
                     /locus_tag="PKU82_11570"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOZ83544.1"
                     /translation="MKDKLSLLEQKKKEALAGGGAKRIDDQHKKGKLTARERIHFLLD
                     EGSFEEIGMLVTHRATDFGLAKEKYLGDGVVTGYGTIHGRLVYVFSQDFTVFGGSLSE
                     THAEKICRIMDLAMQNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVIPQL
                     SAIMGPCAGGAVYSPAITDFIMMVQNTSYMFVTGPNVVKTVTHEEVSAEELGGASTHS
                     TKSGVTHFSCANEMECIQYLKTLLSYIPSNCEEMPPSVAYSEANEVREGLNTIVPENP
                     NQPYDIREVVAQVADENSFFEVHKDFAENIVVGFARLAGRSIGVVANQPMFLAGVLDN
                     HSSTKAARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNAIITNGAKLLYAFSEATVPR
                     ITVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGAAEIIFKKEISKADNPA
                     EKLKEKEQEYIELFANPYKAAERGYIDEVIEPAQTREKLIKAFKMLENKAAKLPKKKH
                     GNIPL"
     gene            3241..>3446
                     /locus_tag="PKU82_11575"
     CDS             3241..>3446
                     /locus_tag="PKU82_11575"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ83545.1"
                     /translation="MKTIFQSLAGIALFLLFHSTSLAQLTRISGKVTDALTGEPIPFA
                     SVVFKKSTIGVNTNIDGIYTLESDT"
BASE COUNT         1028 a          763 c          596 g         1059 t
ORIGIN      
        1 gaggttgcgg tttttatcag gtaacaattg cagcaggtga tactatatgt ggatttaatt
       61 ttggtgtttc tctcaacagt tcttcaatag aaggttatgt ttttgttgac agcaacacta
      121 atggaatcaa agatgcaggc gaagcaggta ttgcttatca acctgtgcag attggaaacc
      181 ttacttccta cactgattta acaggaaaat atgagcgtta tgttccgatt ggaacttata
      241 cagtttccta tacgccaaca ggagtttatg catcttatcc attgacaact gcaagtagta
      301 acagtgtaag tgtcgtaggc gttggtatta cttatcaggg tggaaactat ggtcttgatt
      361 tacccgcagg ttcaacaaat cttgcagtaa atttattacc gcatactact gttacacccg
      421 gcttccctgc atggtacgat attcaggttt gcaacattgg catcaaccca acaggtgcaa
      481 cagttactat gaactacgac ccgggattaa cttatacatt tggtagtcct gtaccaacat
      541 ctgtaaatac aactgcgcac gtcattacct ggaatcttcc aacaatgact tcaggacaat
      601 gcctctatat acaggccgac ttcagtgctt caacttctta tcagattggc gacccaacgt
      661 ttgagttctg cagcgcctat ccaacatcag gaactgatat tgatttaact aataatactg
      721 ataccgttca tcagattgtt acaggctcat gggatccgaa caataaactt tctgttcaaa
      781 ccaacaacaa caatcctgtt cagcagattg tgtcttcaat aaattcaaat caggaaattg
      841 aatatactat caacttccag aatttaggaa cagcacctgc agtgaatgta gtggtagttg
      901 acaacctctc tactgatttg gttgcaagtt cttatcagtt aaccggtgca agccacaatg
      961 tttctgtaag ccgcaatggc atgaatgtta tttataattt tcagaacatt atgcttcctg
     1021 atgccatcag caatgaaccg atgagtcatg gatttgtgtc atataaaatc aatgccgtta
     1081 acggacttgc tgcaggaact caaattgctg atttctcaag aatttatttc gacttcaata
     1141 gtccggtaac tactaacatt gcagttgtta ctatggtgaa tcctactgga attaacgaac
     1201 aaagcgaagc caacttacaa agtgtttatc ctaatcctgt cagtgatgtt gcaactgttc
     1261 agtttacatt agggcaaaat tctttagtag gacttgaatt gtttgatgcg gcaggacgca
     1321 gcaccacatt aatgcccgaa gtcatgatgc aggcaggctt gcataaaaca aatctaaatg
     1381 ccaatcagct ttcagaaggc ttatatcttc ttaagttgac agtcaatgga aaatcatcag
     1441 taatgaaggt tacaattaaa cattaacctt gatttttggt catcattaaa aaagccctct
     1501 tttcagaggg ctttttgttt aaaacaatat tgtaataaat aagaaatatt ccttcctaca
     1561 acggaatatt tccatgcttc ttcttaggca atttggctgc tttattttca agcattttaa
     1621 atgccttaat caatttttct cttgtctgtg caggctcaat cacttcatca atatagccac
     1681 gttctgctgc tttgtatgga ttggcaaaaa gctcaatata ttcctgttct ttttctttca
     1741 gcttttcagc aggattatca gccttactaa tttctttttt gaaaataatt tctgctgcac
     1801 cttttgctcc catcactgca atctctgcag atggccatgc atagttcatg tcggcaccaa
     1861 tatgtttgga gttcatcaca tcataagcac caccataagc tttacgtgtg atgacagtta
     1921 ttcttggcac cgttgcctca ctaaatgcat acaacaattt agctccgtta gtaatgatgg
     1981 cattccactc ctgatcggta ccgggcaaaa atcccggaac atcttcaaac accaacaatg
     2041 gaatattaaa acaatcgcaa aaacgaacaa agcgtgctgc cttagttgat gaatgattat
     2101 ctaaaacacc tgccagaaac ataggctgat tggcaactac gccaatgcta cgaccggcta
     2161 aacgcgcaaa gccaacaaca atattttcgg caaagtcttt atgtacttca aaaaaagaat
     2221 tttcgtctgc tacctgcgct actacctctc gtatgtcgta aggctgattt ggattttcag
     2281 gtacaatcgt attcaaaccc tcacgcactt cattagcttc tgaatatgca actgatggcg
     2341 gcatctcctc gcaatttgac ggaatataac tcagtaaagt tttaagatac tgaatacatt
     2401 ccatctcatt ggcacaagaa aaatgtgtca ctccggattt tgttgaatgg gttgatgctc
     2461 cacctaactc ttctgcactt acttcctcat gggtaactgt ttttacaaca ttaggtccgg
     2521 taacaaacat atatgacgtg ttttgtacca tcataataaa atctgttatt gcaggtgagt
     2581 aaacagcccc acctgcacaa ggtcccataa tggcactcaa ttgaggaata actcctgatg
     2641 ccaatgtgtt acgataaaaa atatctgcat agcctcccaa tgaaacaaca ccttcctgaa
     2701 tgcgggcacc tccactatca tttaagccga ttacaggtgc accattttgc atggccaaat
     2761 ccataatacg gcaaattttt tctgcatgtg tctcactcag ggagccacca aatacagtaa
     2821 agtcctgaga aaaaacatac accaacctac catgaattgt tccgtaaccg gtaacaacac
     2881 catcaccaag atatttctct tttgccaggc caaagtctgt tgcacgatgc gttactaaca
     2941 tgcctatctc ttcaaaactg ccttcatcaa gcaaaaaatg tatccgctct cttgccgtta
     3001 gttttccttt cttatgctga tcgtcaatcc gcttggcacc gccacctgca agtgcttcct
     3061 tctttttctg ttcaagtaat gataatttat ctttcactgc tgtgttaatt attctattgt
     3121 gcaaacttaa aaaactaaat gaagaactgc ccgtttaaca taaaactaaa gccctgtcag
     3181 gcaaggaatc gaactttttt ccgttcttag caaggtaaaa aattctacat aaatcaattt
     3241 gtgaaaacca ttttccaatc gcttgcaggc attgctttat tccttctttt tcacagcact
     3301 tcgttggcac aacttaccag aatttcaggt aaggttaccg atgcactcac cggtgagcct
     3361 ataccctttg ccagtgttgt ttttaaaaaa tcaaccatag gtgtcaacac caatattgat
     3421 ggcatctaca ctttagaatc agatac
//
LOCUS       DAOOOL010000209         3440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3204_length_3440_cov_56.161300, whole genome shotgun sequence.
ACCESSION   DAOOOL010000209 DAOOOL010000000
VERSION     DAOOOL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3204_length_3440_cov_56.161300"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKU82_11580"
     CDS             complement(<1..1038)
                     /locus_tag="PKU82_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOZ83546.1"
                     /translation="MSEKKRLVIVGGGAAGFFSAINAAQLALGLEVIILEKTHKLLSK
                     VRISGGGRCNVTHQCFDNKLLAANYPRGNKELLSCFHQFNAADTIEWFSQRNIKLKAE
                     PDGRMFPDTDSSETIINCFLEEANKSDITIKTGVALTAYSQTKDGQFILHLSDKTTLH
                     ATFLLIASGGHPAASSYSWISNTSNHTILNPTPSLFTFNIPNSPFKGLEGVATEVVLT
                     LPGYNRAEQGPLLITHWGLSGPVVLRMSAWAAEWLNQKNYLTEVSINFLPGFNHDKVL
                     EKFTESKIYYAKTNLVTNKLFSNIPNRLWERLIDCANIDAQIQYAQLSKKQINTLVNL
                     LVDFRIKMSGKT"
     gene            complement(1035..1934)
                     /gene="xerD"
                     /locus_tag="PKU82_11585"
     CDS             complement(1035..1934)
                     /gene="xerD"
                     /locus_tag="PKU82_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOZ83547.1"
                     /translation="MSKVAYINGFSAYIKLEKSLSANSNEAYMHDVELLYEFLQEKHP
                     EKNAEKLTLDLLQQFIQWINERNLSARSQARIISGIKAFYKYLLLENIIADDPTTLLE
                     TPKLGRKLPEFLTIEEIDTMLNAIDLSTAEGQRNRAMLETLYSSGLRVSELISLKKSN
                     SFFDIGFLKITGKGNKERLVPIGTSAIKHINIYEESIRRQLKIKTGNDDILFLNRRGA
                     QLTRVMVFTIIKKLAVKIQLKKKISPHTFRHSFATHLIEGGADLRAVQEMLGHESITT
                     TEIYTHLDRDYLRQEIMQHHPRA"
     gene            2062..2455
                     /gene="rnpB"
                     /locus_tag="PKU82_11590"
     ncRNA           2062..2455
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKU82_11590"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2494..>3440
                     /locus_tag="PKU82_11595"
     CDS             2494..>3440
                     /locus_tag="PKU82_11595"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83548.1"
                     /translation="MKKKLLYISNQHEIVLKFIIFCLTAFLIIKMLPHQVRYKFDYSI
                     SKPWTEKDLIAPFDFAVLKNKDSLNAEKRILLLNATLFFERDTAVKEQVLQRAIARID
                     QIDKHEAELLKQEISILASELYKNGVADESARTVSFGQAKIIKGVDLIPANNFIFEDD
                     AVNELTRIAKEKHIDAPDKAISLLAPLVSNDLTIDKVMTDRFKDELIGTVSETRGMVK
                     KGDLIIAKGEVVTREKYQELESLKSITEEYARPNKHVLIGQVILVVLCLSLLMIFLAQ
                     RRKDIFQSNRRLSIILLMVVFDVFIYTWSLKVEAVSLYIV"
BASE COUNT         1068 a          572 c          677 g         1123 t
ORIGIN      
        1 agttttgcca ctcattttaa ttctaaagtc aaccaaaaga tttactaatg tgttgatttg
       61 tttttttgat aattgagcat attgaatttg tgcatcaatg tttgcacaat caattaaacg
      121 ctcccagagc ctgttcggta tattggagaa taatttatta gttactaaat tagtctttgc
      181 ataataaatt ttgctttcag taaatttttc taagacttta tcatgattaa atcccggtaa
      241 aaaatttatg cttacttcag taagataatt cttttgattt agccattctg cagcccatgc
      301 agacatgcgc aacactaccg gaccgctcag cccccaatga gtgattagta gtgggccttg
      361 ctcagctctg ttgtaacccg gtaatgttaa cacaacttct gtggcaacac cttcaagacc
      421 tttgaatggt gaatttggga tattgaaagt gaaaagtgat ggagtaggat ttaatatagt
      481 atgattggat gtattcgaaa tccacgaata cgaagatgct gcaggatgcc cacctgaagc
      541 aataagcaga aaggtagcat gtaaagtagt tttatcagaa agatgcaaaa taaactgacc
      601 atcttttgtt tgagaatatg cagtcagcgc tacacctgtt ttgatagtaa tgtctgactt
      661 gttggcttcc tccaagaaac aattgataat ggtttctgag gagtcagtgt caggaaacat
      721 tctaccatca ggttctgctt ttaatttgat atttcgttga ctgaaccact caatagtgtc
      781 agctgcattg aattgatgaa agcaacttaa taattcttta tttcctcgag gataatttgc
      841 agccaacagt ttgttgtcaa aacattgatg agtcacattg cagcggccac cacctgaaat
      901 tcgtactttt gacaaaagtt tatgtgtttt ttccagaatt attacctcta agcccagagc
      961 aagttgtgca gcattgatag cagaaaaaaa accggcagcg ccaccaccaa ctataacaag
     1021 tcgtttcttt tcactcatgc acgtggatga tgttgcataa tttcctgacg aagataatct
     1081 ctgtcaaggt gtgtgtaaat ttctgttgtg gtaatacttt catgaccgag catttcctgc
     1141 acagcacgta agtcagcacc accttcaatc aggtgtgtgg caaaactatg tctgaacgta
     1201 tgggggctga tttttttctt aagctgaatt ttaactgcta atttcttgat gatggtaaac
     1261 accataacgc gtgttagctg agcacctctc ctgtttagaa aaagaatatc atcattgcct
     1321 gtcttgattt taagttgtct tctgatagac tcttcataaa tattgatatg tttaattgca
     1381 gaagtgccaa taggaaccaa ccgttcttta tttcctttac ctgtgatttt taaaaatcca
     1441 atgtcgaaaa atgaattaga tttttttagg gaaataagtt cactgacacg taatcccgaa
     1501 ctataaagtg tttctaacat ggctctgttg cgctgacctt ctgctgtact taaatcaata
     1561 gcatttagca tggtgtcaat ttcttcaata gttaaaaatt caggaagttt tctgccaagt
     1621 ttaggtgttt ctaataatgt tgtcgggtcg tcagcaataa tattttcgag taagagatat
     1681 ttatagaatg ctttaatacc tgaaataatt cttgcctgtg aacgagcgct aagattccgt
     1741 tcatttatcc actgaataaa ttgttgtaag aggtctaatg tgagcttttc agcatttttt
     1801 tccgggtgtt tttcctgaag aaattcataa agcagttcca catcatgcat ataggcttca
     1861 ttagaattgg cagataatga tttctccaat ttaatgtatg ctgaaaagcc attaatgtat
     1921 gccactttac tcattgtgct aaaatagcta accgaaactt aactcaagag tttggtttgg
     1981 tgtaattaag tataaaaaat caactattgc atgtttattg attttaacta agaatcaagg
     2041 ataataaacc gtatttttgc atcggcaagc cgctctatcg ctcttaactc gtttaagaga
     2101 ggaaagtccg ggcaacaaag agtatcctgc ctcctgttaa aggagggtgt agttttagtg
     2161 atgaagctat aacagaaagt gccacagaga aaataattcc accggcaatg tattgcatgg
     2221 tggtaaaggt gaaaacgtga ggtaagagct tacgggcata ttcagtaatg aaaatgcaag
     2281 gtaaacctca ggagttgaaa ggcaaaataa atcacgaaca ggcaatggct cgttgccgtt
     2341 tgttcagcaa tgaacatctt cgtgaagggt atgccgcaaa cagacaaatg ataggggatt
     2401 tgaatgctga aaagtattca gataacagaa tccggcttac aggcttgctt tttatttttt
     2461 ctgctgcatt gctttttaat aatttagcag ccaatgaaaa agaagctcct ttacatcagc
     2521 aatcagcatg agatagttct taaatttatt atcttttgtc ttacagcatt tttgataata
     2581 aaaatgttgc cacatcaggt aaggtataag tttgattatt ctatatctaa accctggaca
     2641 gagaaagatc tgattgcacc gtttgatttt gccgttctga agaataaaga ttcgctgaat
     2701 gcagagaaga gaattttact tctcaatgca acactgtttt ttgaacgtga tactgccgtt
     2761 aaggaacagg ttttgcaacg ggcaatagca cgtattgatc aaatagataa acatgaagca
     2821 gagttattga agcaggaaat ttcaatactt gccagtgaac tttataagaa tggtgtagct
     2881 gacgaaagtg cacgtactgt ctcctttggt caagctaaaa taataaaagg tgttgacctg
     2941 atacctgcta acaattttat ttttgaagat gatgccgtta atgagctgac gcgtattgct
     3001 aaagaaaaac atattgatgc tcctgacaag gctatttctt tacttgcccc tttggtgagt
     3061 aatgatttga ccattgataa agtaatgaca gaccgtttta aagatgaatt gattggtacg
     3121 gtttctgaaa cgcgcggaat ggttaaaaag ggcgatttaa ttattgccaa aggtgaagtt
     3181 gtgacacgcg aaaaatatca ggagttggaa tctctgaaat caattactga agaatatgct
     3241 cgtcccaata aacatgtact tatcggtcaa gtaatattgg ttgtattgtg tctttcttta
     3301 ttgatgatat ttcttgcgca aagaagaaaa gatatttttc agtcaaacag aaggctaagt
     3361 attattttat taatggtagt ttttgatgta tttatataca catggtcgtt gaaagttgaa
     3421 gctgtgagtt tatatattgt
//
LOCUS       DAOOOL010000210         3387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3281_length_3387_cov_44.058523, whole genome shotgun sequence.
ACCESSION   DAOOOL010000210 DAOOOL010000000
VERSION     DAOOOL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_3387_cov_44.058523"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PKU82_11600"
     CDS             complement(<1..1003)
                     /locus_tag="PKU82_11600"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOZ83549.1"
                     /translation="MKQINKKGRSILIMLAIMLSGLSSMAQNCNANFQYFAGPGGVVT
                     FMDSSFASGNITGYTWTFGDGTSGTGQQVSHTYNGVGPYTVCLFITTSNGCSDSTCAL
                     VSLNPCNISGVILYDSLQQTLTVTASGGVAPYTYNWTNGQTGMSISVGNPGMYCCVIT
                     DANGCAYTACYMVGGSGCSAAFITNIMGNVVVFGNNSQSFASLLWNFGDGTTSVATSP
                     THTYAQAGTYYPCLTVYDGNGAICSQYCDTIVIVPQAGNTVICGNVFQDMNANGFNDN
                     EPGFANTPVMIWGSGMQYTAMPDSNGNYSINVPPGTYNIVYCVQQPYSLTLPLDSGGC
                     "
     gene            complement(1128..2081)
                     /locus_tag="PKU82_11605"
     CDS             complement(1128..2081)
                     /locus_tag="PKU82_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOZ83550.1"
                     /translation="MRTAILIATIFTTLNISCATGQKNSSSNEPDMKEQLKNGTNIYF
                     ENKTISTDLNFTNLLQPVVESNGINRCYVNSAITFKQCTFKGKITGYATDQSGKSTMT
                     YFQKSVTFIDCIFEDDINLRGAIFSAPANFQGCSFVKKADFQECHFYTDALLNETKYR
                     EEAFFQNAVFMKKMILMNARFESNVYFQNAVFYFDAQFSNAEFQKYADFTVCTFQMGV
                     FFNYCKFQQQAVFSSSVFSKRFEILSTTAQQLDMRKCHFGFVPKLMKSNIVSELDFSG
                     SHFTDGIPDLKETSAATILASEIVLCDKSYSQEQFLKASGK"
     gene            complement(2184..>3387)
                     /locus_tag="PKU82_11610"
     CDS             complement(2184..>3387)
                     /locus_tag="PKU82_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOZ83551.1"
                     /translation="PYTNLGNCANSGVQSNWTQEANQRKIKNYRKVSPNIQSIKEQLA
                     NKIPVVIGAKLSDNFMTWNSDAVYNSNSTYNQVGQHSYHAIIISGYDDSKGANGAFKV
                     VNSWGTNWGDVGYIWIDYNFLVNEFVFDQNFYTATTDGGTNPPSPTPTPSGGVDLASW
                     VFGDETTYGTSQTQYYNERVVYWNLYNIGTSGAASSSNWSLYYIYFNAYDANDYGVLF
                     YDEFNTSIAQNTYQVDPNDPNHIILNIDIPANNDLGNVGFGTQQLYQVYYTPSTLNGE
                     YYIAMIADAENAFTEDNEQNNFFYTTDQYPKTFQNGYSGRITAQMQSTNATNYVEYKF
                     INKLQPSKHNLTSSIYNTVVTKQRPNAYSSEEIGQMLKWKKKTGDLDRKLAEFKMRQK
                     GNGGFGAK"
BASE COUNT         1047 a          608 c          622 g         1110 t
ORIGIN      
        1 cgcaacctcc tgaatcaagt ggtaaggtaa gactgtaagg ttgttgaaca cagtaaacaa
       61 tattataagt tccgggagga acattgatgc tgtaattacc atttgaatcg ggcattgcag
      121 tgtattgcat accggatccc caaatcatta ccggtgtatt ggcaaagccc ggttcattat
      181 cattaaatcc attggcattc atgtcctgaa agacattgcc gcaaataact gtattgcctg
      241 cttgtggtac aattacaatc gtatcgcaat actgtgaaca tatagcaccg ttgccatcat
      301 aaactgtcag acaaggataa taggttcctg cctgagcata agtgtgtgtt ggtgatgtag
      361 caacagaagt ggtgccatca ccaaagttcc agagtagtga tgcaaaactc tgtgagttgt
      421 taccaaacac cacaacatta cccataatgt tggtgataaa tgctgcactg caaccggagc
      481 cacccaccat atagcatgct gtataggcac agccgttagc atcggtaatc acacagcaat
      541 acattcctgg attaccaaca gaaatgctca ttcctgtttg accatttgtc cagttgtagg
      601 tgtaaggtgc tacaccgcct gatgctgtaa ctgttaatgt ttgttgcagt gaatcataaa
      661 gaatgacacc ggatatatta caggggttta aactaactaa tgcgcaggta gaatcgctgc
      721 aaccattaga tgttgtaata aataaacata ctgtgtaagg acccacacca ttataagtat
      781 gcgatacttg ctgacctgtt cctgatgtgc catcaccaaa agtccaggta taaccggtga
      841 tattgcctga tgcaaaactt gagtccataa aagttactac tccaccgggt ccggcaaagt
      901 attgaaagtt tgcattacaa ttttgtgcca tactgctcaa accggagagc atgatggcaa
      961 gcatgataag gatgcttctt ccttttttgt taatctgttt catgatattt gaattttgat
     1021 acaaagaaaa tgtaacggta tattactgca aaacggattt tgaataaatc ttacttgctt
     1081 aaagtagcta aaatgttcta ttttcaataa tgaaatcaaa aataagctta cttacctgag
     1141 gcttttaaaa actgctcctg agagtaactt ttatcgcata aaacaatctc tgatgctaat
     1201 attgttgcag cagaagtttc ttttaagtca ggaatgccgt ctgtaaaatg gcttccgctg
     1261 aagtcaagtt ctgaaacaat attacttttc atcaatttgg ggacaaaacc aaaatgacat
     1321 tttctcatgt ccaattgctg agctgtggta ctaagtattt cgaagcgttt actaaacaca
     1381 ctactactga aaactgcctg ttgttgaaat ttgcagtagt tgaagaaaac acccatctga
     1441 aaagtgcaaa cagtaaaatc agcatatttc tgaaactctg cattgctaaa ctgcgcatca
     1501 aaataaaaaa cagcattttg aaaatataca tttgattcaa atcgtgcatt cataagaatc
     1561 atttttttca taaatacagc attttgaaag aatgcttctt cgcgatattt ggtttcattt
     1621 aataaagcat ctgtataaaa atgacattcc tgaaaatcgg ctttttttac aaagctacaa
     1681 ccttgaaaat ttgccggagc agaaaatatt gctcctctta aatttatatc atcttcaaaa
     1741 atacaatcaa tgaatgttac acttttttga aaataagtca ttgttgactt gcctgattga
     1801 tcagtagcat atcctgtaat ttttcctttg aatgtacact gtttaaaagt aattgcagag
     1861 ttgacataac atcggtttat gccattgctt tcaacaacag gttgcagcag atttgtaaaa
     1921 tttaaatcag tactgatagt tttattttca aaataaatat tagttccgtt ttttagttgt
     1981 tctttcatat ccggttcgtt tgaagagctg tttttttgtc ctgtagcaca ggaaatattt
     2041 agtgttgtaa aaattgtagc tataagaatt gctgtacgca taatattttt tttacaagtt
     2101 taaaaatcat ttggtgaaac agaaaaaatc ccggcagaaa ttttcttacc gggatgatat
     2161 tgtttgcggt ttaaatgttg aatttatttg gcaccaaagc ctccgttgcc tttttgacgc
     2221 attttaaatt ctgctaattt acggtcaagg tcaccggttt ttttcttcca tttaagcatc
     2281 tgtccaattt cttcagatga ataagcattt ggacgttgtt tggtaacaac agtattatag
     2341 atgcttgaag ttaaattgtg ctttgatggt tgaagtttat taatgaattt gtactctaca
     2401 tagtttgttg cattggttga ttgcatttgc gcagtaatac gcccactgta gccattttga
     2461 aatgtttttg gatactgatc agttgtgtag aagaagttat tttgttcatt atcttctgta
     2521 aatgcatttt ctgcatcagc aatcattgca atataatatt ctccattcag agttgaaggg
     2581 gtgtagtaaa cctgatacaa ttgttgtgtt ccaaaaccaa cattaccaag atcgttatta
     2641 gcaggtatgt caatattcag aataatgtga ttgggatcat tcgggtcaac ctgataagta
     2701 ttttgagcaa tagatgtatt gaactcatca tagaaaagca caccataatc attggcatca
     2761 taagcattaa agtaaatata atacaaactc cagtttgaag aacttgctgc tcccgaagtt
     2821 ccaatgttgt atagattcca ataaacaaca cgttcgttgt aatattgtgt ttgtgatgta
     2881 ccataagttg tttcatcacc aaacacccaa gaggctaggt caacgcctcc tgaaggtgtt
     2941 ggagtaggtg aaggaggatt tgttcctcca tcggttgtgg cggtataaaa gttttgatcg
     3001 aatacgaatt cattcacaag aaaattataa tctatccaga tatatccaac atcgccccaa
     3061 ttggttcccc atgagtttac aactttgaat gcaccattgg cacctttact atcatcataa
     3121 ccggaaatga taattgcatg ataggaatgt tgtccaacct gattgtaagt tgaatttgaa
     3181 ttgtaaacgg catcgctgtt ccaagtcatg aaattgtcgg aaagcttagc tccaatcact
     3241 accggaattt tattggctaa ttgctcttta atactttgaa tattcggact gacttttctg
     3301 taatttttaa tcttgcgttg attcgcttcc tgtgtccagt tactttgcac accggagttg
     3361 gcacaattgc ctagattggt atatgga
//
LOCUS       DAOOOL010000211         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3301_length_3377_cov_61.472908, whole genome shotgun sequence.
ACCESSION   DAOOOL010000211 DAOOOL010000000
VERSION     DAOOOL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_3377_cov_61.472908"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKU82_11615"
     CDS             complement(<1..1088)
                     /locus_tag="PKU82_11615"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOZ83552.1"
                     /translation="MFSTENIHAQTLPKDSLSTDTLAKDTIKTGVSNDLKSKVHYTAD
                     DTIEFDIEHEKVFLYEKAQVNYENIELKAAYIEVDWNTKNVFSAGLRDSTGKLYGMPV
                     FKEGDDEFKADTMRYNFDTKKGKVYHVVTQQGDGFIHGAIVKKIEESSYIRYGKYTTC
                     SLDTPHYYIAANKLKVIPNNKIITGPAYLIIGDVPAPAAIPFGLFPNTKGRSSGILFP
                     AYGESNALGFFFKNGGYYFGISDHVDLQLTADIYTLGSWAVKATSRYNNRYHYNGNLS
                     FLYSIIKVSEKDLPDYSRSKEFFINWSHLQDPKARPNGIFSASVRAGSNDYYTRNISS
                     ASNYLTNTFNSSISYSRSFFNKQLNFSSA"
     gene            1308..2630
                     /locus_tag="PKU82_11620"
     CDS             1308..2630
                     /locus_tag="PKU82_11620"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOZ83553.1"
                     /translation="MSASNGTYKEPYRIKTIVIDPGHGGHDSGCLGASSKEKQIALEV
                     ALKLGEMITQKYPDVKVIYTRKTDEFVELHERAAIANRNHAELFICIHCNSGSPAAFG
                     AETYVMGLHKTEDNLNVAKRENAAVLLEKDYKQKYDGFDPNSPEANIVFTLYQNAYLT
                     QSLKFASAVQQQFEVYAGRYNRGVKQAGFLVLYKTAMPSVLIETGFLTNRTEEKYLLT
                     EKGQNAVATSIFRAFMEYKLDMETPGGKIPQGGSENDLNKNVIEVKNKQSDKESSQPQ
                     KKVTDSEKNPPTTNSTKENHEVNKDTTKKNTDSADTQKETPPSENYKTETAVKPVASK
                     EKITTAEVKKETAHTDKLPDFFYTVQLAAMPDASTEELKRFEDLTDIKTFKAENGMIR
                     VCQGVYTTHAKALAAQSQVRAKGYKDAFVTPYYKGKRISNKEAEAIKK"
     gene            2642..>3377
                     /locus_tag="PKU82_11625"
     CDS             2642..>3377
                     /locus_tag="PKU82_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOZ83554.1"
                     /translation="MKISNEVKVGIAFTLTIAGFFWGMNFLKGTDLFSTSNKYYVVYD
                     DVNGIVKSNPVIMNGFKIGQVDKIVYAEDKSGRLIVTLRVDDNVFIPKSSNAAIVSSD
                     LLGSKAIELEMSEDKTPAKNGDTLVGNVQSGLTDQIKPLKNKAEGLVTSLDSLSYALT
                     QLINERSRNSLNNIFANLENTTEGLDKMVNSSNSKLNSMLSNIDGVAGNLNNKQSDIN
                     GILKNVNSLSDSLSRLQLNLTIEKLNK"
BASE COUNT         1170 a          583 c          651 g          973 t
ORIGIN      
        1 gcagatgaaa agttaagttg tttattgaaa aaagaacgtg aataagaaat tgatgagttg
       61 aaagtattag taagatagtt tgatgcgctt gaaatatttc ttgtgtaata atcgttggac
      121 ccggcacgga cagaagcgga gaaaattccg ttaggccttg ctttaggatc ttgcaggtga
      181 ctccagttta tgaaaaattc tttgctacgg ctatagtctg gtaaatcttt ctcacttact
      241 tttatgatag agtataggaa cgataaattt ccattgtaat gataacggtt attgtatcgt
      301 gaagttgctt ttacagccca actacctaaa gtatagatgt ctgctgtaag ttgcaggtca
      361 acatggtcgc taataccaaa ataatagcct ccgtttttaa agaaaaatcc aagtgcattg
      421 ctttcaccgt atgcgggaaa taaaattccg gaactacgac ctttggtatt gggaaataat
      481 ccgaatggaa tggctgcagg tgcaggaaca tctccaataa tcagataagc aggcccggta
      541 ataattttat tgtttggaat tactttgagt ttgtttgcgg caatgtaata gtgtggtgta
      601 tccaaactac aggtagtgta tttgccataa cgtatatagc tgctctcctc tattttttta
      661 actattgcac catgaataaa gccatcgccc tgctgtgtta cgacatgata aaccttgcct
      721 ttcttggtgt caaaattata gcgcattgta tcggctttaa actcatcatc accttcttta
      781 aaaacaggca ttccatagag ttttcctgtt gaatcgcgca atccagccga aaaaacattt
      841 tttgtattcc agtccacttc aatataagca gccttcagct caatattttc ataattgacc
      901 tgagcctttt catacaaaaa aactttttca tgttcaatat caaattcaat agtatcgtca
      961 gcagtgtaat gaactttgga ctttaaatca ttactcacac cggttttaat ggtgtctttg
     1021 gcaagagtat cagtagataa tgagtctttt ggaagcgttt gtgcatgaat attttcagtt
     1081 gaaaacacaa tgaccgccag gcaccacaat attgcaatcg ttaataagtt tttaaaaacg
     1141 gtcatttaca aaaaaaggag atggagttaa aaacgatatt tttggagccg caaaggtaat
     1201 tgaacagaat ttaacaccat ccaaagaaac gcagaatcat gttgtttatt tataagaaga
     1261 taaaaatacg gctctttttt acactttcac tgttgttttt aatctgtgtg tcagcgtcta
     1321 atggaacata taaggaacca tatcgcataa agaccattgt tattgacccc ggtcatggtg
     1381 ggcatgattc aggctgtctg ggtgcttcat ctaaagaaaa acaaatagca cttgaagtgg
     1441 cacttaaatt gggtgaaatg attacccaaa aataccctga cgtgaaggtt atttacacaa
     1501 gaaaaacaga tgagtttgta gaattgcatg agcgtgctgc tattgccaat cgtaatcatg
     1561 ccgaactttt catctgcata cattgcaact ccggtagccc agcagctttt ggtgcagaaa
     1621 cctatgttat gggtttacat aaaacagaag ataatcttaa tgttgccaaa cgcgaaaatg
     1681 ccgctgtgtt gttggaaaag gactacaagc aaaagtatga tggctttgac cccaactcgc
     1741 ctgaagccaa tattgtgttt acattgtatc agaatgctta tctgactcag agtttgaagt
     1801 ttgcctctgc cgttcaacaa cagtttgaag tctatgcagg gcgttataac cgtggtgtta
     1861 agcaggctgg ttttttggta ctttataaaa ctgctatgcc atcagttctg attgaaaccg
     1921 gatttttaac caacagaaca gaagagaaat atttacttac tgaaaaaggt cagaatgctg
     1981 ttgctacaag tattttcaga gcatttatgg agtataaatt agatatggaa actcctggcg
     2041 gcaagatacc gcagggaggc tcagagaatg acttaaataa aaatgtaatt gaagtaaaga
     2101 ataagcaatc agataaggaa tcttctcagc cacagaaaaa ggttacagac agtgaaaaaa
     2161 atccccccac tacaaacagc accaaagaaa atcacgaagt aaataaagat actaccaaga
     2221 aaaacacaga cagtgctgat actcaaaaag agacaccccc atcagaaaat tacaaaacag
     2281 aaactgctgt taagcccgtt gcttcaaaag aaaaaattac cactgctgag gtgaaaaagg
     2341 aaactgcaca tacagataaa ttacctgact ttttttatac cgtacaactt gctgcaatgc
     2401 ctgatgcatc aacagaagaa ttaaaacgat ttgaagacct gactgacatt aaaacattta
     2461 aagccgaaaa tggaatgata agagtttgtc agggtgttta tacaacacat gcaaaagcat
     2521 tggcagcaca gtcacaggtt cgggcaaaag gatataaaga tgcatttgtt actccttact
     2581 ataaaggcaa acgaatttca aataaagagg cagaagcaat taaaaaataa attcagatca
     2641 cgtgaaaata tctaacgaag taaaagtagg catagcattt acacttacca tagccggttt
     2701 tttctggggt atgaactttt taaagggaac agatttgttt tctacctcaa acaaatatta
     2761 tgttgtttat gacgatgtaa atggtattgt aaaatcaaat ccggttataa tgaacgggtt
     2821 taaaatcggt caggtagata agattgttta tgctgaagat aaaagtggaa ggttaatagt
     2881 gacacttcgt gttgatgata atgtatttat tccaaaatca tcaaatgcag caattgtttc
     2941 aagtgatttg ttaggctcaa aagccataga gcttgaaatg agtgaagata aaacacctgc
     3001 taaaaatggc gacaccttag ttggaaatgt tcagtcagga ttgacagatc agataaaacc
     3061 actaaagaat aaagcggaag ggttggtgac ttctcttgat tcattatcgt atgctttaac
     3121 gcaactcatc aatgaaagat cacgcaacag tttgaataat atttttgcta accttgaaaa
     3181 tacaactgaa ggtttggata aaatggtgaa cagttcaaac agcaaactca acagtatgct
     3241 ttcaaatatt gatggagtgg caggtaacct aaacaacaaa caatccgaca tcaatggtat
     3301 tttaaaaaat gttaactcgc tcagtgattc actcagcagg ttacagctaa acctcactat
     3361 tgaaaaactc aataaaa
//
LOCUS       DAOOOL010000212         3359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3334_length_3359_cov_42.257869, whole genome shotgun sequence.
ACCESSION   DAOOOL010000212 DAOOOL010000000
VERSION     DAOOOL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3334_length_3359_cov_42.257869"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PKU82_11630"
     CDS             complement(<1..1003)
                     /locus_tag="PKU82_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83555.1"
                     /translation="MSTLKHHLWTMLCIVLLSACKNQNTVIVTNKNFEDVVAQQQNLI
                     FTFDKDLVDDSLLNRWDSIQYIAFTPTVRGLFKWNTNRELMFSPETSFGPATFYKAVV
                     TDKILFGNRLLSFDAPAAYDFHTPLTQLEGLHAHWALSTQQNSIVLLAKLDFNYPVPS
                     AQMKDLAQLKINEKVYPFTVVTNEASTEVVLEINNIQPADFKKVNVSVSINKGLACNG
                     SNYKTENVLVKDADVPLPDRLTVMNVSGEYDNTQPVIHVYTNQAVDLKQIETYVTVQP
                     KIDFTTEAANDGFLIKGAFDPGQSYQLTVKKDLKGLIGGMLDQDFIATVPFGEMQPSI
                     "
     gene            complement(1418..2398)
                     /locus_tag="PKU82_11635"
     CDS             complement(1418..2398)
                     /locus_tag="PKU82_11635"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOZ83556.1"
                     /translation="MREIQFREALREALSEEMRRDPNVFLMGEEVAQYNGAYKVSQGM
                     LDEFGAKRVIDTPIAELGFAGIGVGAAMNGLRPVIEFMTFNFSLVAIDQVINAAAKMK
                     SMSGGQYGVPIVFRGPTASAGMLSSQHSQAFESWYANCPGLKVIVPSNPKDAKGLLKA
                     AIRDDDPVIFMESEQMYGDKGEVPEGEYLMPIGVADIKRPGNDVTIVSFGKIIKTAYK
                     AAEELAKENIDVEIIDLRTVRPIDYATVIESVKKTNRMVIVEESWPLANIATEVAFKV
                     QREAFDYLDAPVLRVTGGDVPLPYAPTLIDAYLPNPERIIRAVKKVMYLD"
     gene            2559..2753
                     /locus_tag="PKU82_11640"
     CDS             2559..2753
                     /locus_tag="PKU82_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83557.1"
                     /translation="MKFYEKYPQLKEKEFLAELLSKSVFATMALENQQVPMKKIQEIV
                     KSLLNEKESKGGQFFSDQIQ"
     gene            complement(2715..3296)
                     /locus_tag="PKU82_11645"
     CDS             complement(2715..3296)
                     /locus_tag="PKU82_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ83558.1"
                     /translation="MSDDTNQTSYPEENKLGITDKRFINEIESKGVATAEYFIFELDT
                     DIIISSQLICEIHRIAFSELYEWAGKWRTVSVYVGNLIPPEPGQVVQLMYQYIDNLNF
                     KLINSKHTQDHLDCLIFAHYEFVRIHPFNNGNGRTARILMNLVALKLGYKPLELYHRQ
                     GDNRNIYINALRLADKGNFELLLNLIRKELTPF"
BASE COUNT         1048 a          649 c          587 g         1075 t
ORIGIN      
        1 atatggatgg ctgcatttct ccaaatggta cagttgcaat aaaatcctga tctaacatgc
       61 caccgattaa tcctttcaga tcttttttaa ctgtaagctg ataggattgt cctgggtcga
      121 atgctccttt gattaagaaa ccatcgttgg ctgcttctgt ggtaaagtct atttttggct
      181 gaacagtaac gtaagtttca atctgcttca aatcaacagc ctgattggtg taaacatgaa
      241 tgacaggttg cgtgttgtca tattcgcctg atacattcat aactgtaagc ctgtcgggca
      301 gtggcacatc ggcatcttta acaagaacat tttctgtttt atagttgctg ccattacatg
      361 ccaaaccttt attaatagaa acgctgacat ttactttttt aaaatctgca ggttgtatgt
      421 tgtttatttc cagaaccact tctgttgaag cttcgttggt aacaacggta aaaggataaa
      481 ctttttcatt aattttcagt tgagcaaggt ctttcatctg cgctgacgga acaggataat
      541 taaaatcaag ttttgccagc aacacaatgc tgttttgttg tgtagaaagt gcccagtgtg
      601 catgaaggcc ttcaagctgc gtcagtggcg tatgaaaatc ataagcagca ggtgcatcaa
      661 aagaaagcag tctgtttcca aaaagaattt tatccgtaac aacagcttta taaaaggtag
      721 cggggccaaa agatgtttct ggcgaaaaca tcaactcgcg attggtattc cacttaaaca
      781 atcccctgac cgttggtgtg aaggcaatat actgtatgct gtcccagcgg ttaagcaagc
      841 tgtcgtcaac aaggtcttta tcaaaagtaa aaatgaggtt ttgttgttgc gctacaacat
      901 cttcaaaatt tttgttagta acaataaccg tgttttggtt tttgcatgcc gatagcagaa
      961 caatgcatag cattgtccat aaatggtgtt taagggtact cataatgcag attttacctt
     1021 tataaaaatc ataaaggtaa cggatgaaat gttatagaag tataggagga ataaaatttt
     1081 taaacttccc aataattgaa aagcatgatg cctacaatat ttcagtgatt aaatgccttt
     1141 ataatacaaa taaatctcaa caaaaaagac tttaatttaa tgctttgata tactaagaaa
     1201 tccagatcta ttcttttaca aattttgcat attctttaaa accattgtaa ctaacctcaa
     1261 caatgtaaat gccttttgca agatttgcta tgggaacgat tactttttgt tgtacaaaac
     1321 tttgcctgta aacttcttta ttcagtacat taaacaaacg taaattgtat gatgttcgta
     1381 cagcctttac ctgatataca ccctgtgcta aatttgttta gtccaaatac atcaccttct
     1441 tcacagccct gataatacgt tcggggtttg ggaggtaggc atcaataagt gtaggggcat
     1501 agggcaatgg cacatcgcca cctgttacac gcagcaccgg tgcatcaaga taatcgaatg
     1561 cttcgcgctg tactttaaat gcaacttctg tagcaatgtt tgccagcggc cacgattctt
     1621 caacaattac catgcgattg gtttttttaa ccgactcaat aactgttgca tagtcaatag
     1681 gacgcaccgt acgcaggtca ataatttcaa cgtctatatt ttctttggct aactcttctg
     1741 ctgccttata tgcagtcttt ataatttttc cgaacgatac aatggtaaca tcattgccag
     1801 gacgtttgat gtctgcaacg ccaataggca tcaggtattc accttcaggc acttcgcctt
     1861 tgtcgccata catttgttcg ctttccataa aaattacagg atcgtcatca cgtattgcgg
     1921 ctttaagaag tcctttggca tctttagggt ttgagggaac aataactttt agtccggggc
     1981 agttggcata ccaactttca aatgcctgtg agtgttgtga actgagcata cctgccgatg
     2041 cagtaggccc gcgaaaaaca ataggtactc catattgtcc gccactcata cttttcattt
     2101 tagctgctgc attaataacc tgatcaatgg caaccaatga aaaattaaag gtcataaact
     2161 ctataacagg acgcaaacca ttcatggcag caccaacacc tattcctgca aaacccaatt
     2221 ctgcaatagg tgtgtcaata acacgtttgg caccaaactc atccaacatg ccttgcgaaa
     2281 ctttatatgc accgttgtat tgtgcaacct cctcgcccat taaaaaaaca ttggggtccc
     2341 tgcgcatttc ttcgcttaag gcttctcgca gggcttctct gaattgtatt tcgcgcataa
     2401 tattgttcta tgccacaaat gtacataaaa tcaatacagt gttcaaaaac agtgatggaa
     2461 tatcatgaat tgttatttgt ctaaccaatt attctaaaag caacttgaat atatttaaat
     2521 tgttttgcga aaaagtttta tttatctttg taatgcaaat gaaattttac gaaaaatatc
     2581 cacaattaaa agaaaaagaa tttttagcag aactcttgtc aaaatctgtt tttgccacaa
     2641 tggctttgga aaatcaacaa gttccgatga aaaaaattca ggagattgtt aaatcacttc
     2701 tgaatgaaaa ggaatcaaaa gggggtcaat tcttttctga tcaaattcaa taatagctca
     2761 aaatttcctt tatcagccaa tcttaatgcg ttgatataaa tattacgatt atcgccttgc
     2821 cggtgataaa gttcaagagg tttgtacccc aatttcaatg caacaagatt catcaatatc
     2881 cttgccgtac gcccgttacc attattgaaa ggatgaattc tgacaaactc ataatgagca
     2941 aatattaaac aatcgagatg atcctgagtg tgtttggaat tgattagttt aaaatttaaa
     3001 ttatcaatgt actgatacat taattgaaca acctgacctg gctcaggcgg tatcagatta
     3061 ccgacatata ctgatactgt tctccattta ccggcccatt catataattc tgaaaatgca
     3121 atcctgtgaa tttcgcatat taattgagaa gaaataataa tatccgtgtc gagttcgaaa
     3181 ataaaatatt cagcagttgc tacacctttc gactcaatct cattaatgaa tcttttgtct
     3241 gttattccta gtttattttc ttcaggatat gatgtttgat tggtatcatc actcatgctt
     3301 acttcaatta atcattcatt cttttagtta gatgttaaat taaagataac aacaaacac
//
LOCUS       DAOOOL010000213         3303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3415_length_3303_cov_69.746613, whole genome shotgun sequence.
ACCESSION   DAOOOL010000213 DAOOOL010000000
VERSION     DAOOOL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_3303_cov_69.746613"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKU82_11650"
     CDS             <1..715
                     /locus_tag="PKU82_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOZ83559.1"
                     /translation="VCIAAANAGATSQDLKTGYIVGATPRYQQMALFIGAIVSSVVIG
                     FTVKYLDTPTADLTAQGIHHAIGEKYAAPQATLMATLIKGLLSYNLDWQFVLVGVFIA
                     ITMELCGVKALSFAVGAYLPLSTTLPIFAGGMVKGIVDWNAKRKHKKAEGDDELGKGN
                     LFATGLVAGGALAGVVVALLSVDEGVYAALEKVNMEHSIAAVIGEDGYQLLGVGFFTF
                     MSVILYRIAIKPEKKTLNN"
     gene            732..1250
                     /locus_tag="PKU82_11655"
     CDS             732..1250
                     /locus_tag="PKU82_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOZ83560.1"
                     /translation="MKRNLIGVVFSFIILTGYVSCSQPSKIEHKIKWMDFESAVAASQ
                     KNPKKLFIDVYTDWCGWCKKMDASTFMDDSVAAFINANYYPVKFNAESKDTIHFFDQV
                     FVFVPEYKSHELAISLLNRQMGYPSYAFLDEKFAAIKVQQGYMPVETLMPLLKYYAHD
                     AYKTKTFEEFTK"
     gene            complement(1303..2697)
                     /locus_tag="PKU82_11660"
     CDS             complement(1303..2697)
                     /locus_tag="PKU82_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOZ83561.1"
                     /translation="MKNRYIDLIQQSYDFPQEGFEVIDDSLHFNGINLMDVIKQYGTP
                     LKITYLPKISSQIQKAKRLFKVAMAKVDYKGSYYYCYCTKSSHFQFVMEEALKNDIHI
                     ETSSAFDIPIIRSLYESQKINKDIYILCNGFKREGYIEGIVNLIKDGFTNVIPILDNV
                     DELQEYEKAMPGKKFKIGIRIASEEEPNAEFYTSRLGIRQRDIVDFYKNTIKKNPNVE
                     LKLLHFFINTGIRDTAYYWNELQRNIAFYADLKKICPELTALDIGGGFPIKTKLEFNF
                     DYQYMADEIVMQIKSACDQRKVPVPHLFTEFGSFTVGESGAILYSVTGIKQQNDKELW
                     YMIDSSFITTLPDTWGIKQKFILLAINHWDKEQQRVNLGGITCDGEDYYIMESVKNRV
                     FMPRPTPDEPLYLGFFHTGAYQDSLGGYGGIQHCLLPAPKHVLIDKTEEGELSMWLFA
                     KEQSSKSMMKILGY"
     gene            2777..>3303
                     /locus_tag="PKU82_11665"
     CDS             2777..>3303
                     /locus_tag="PKU82_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOZ83562.1"
                     /translation="MASKIKFIEVKSEIGAGTRGASLGVDAIKIAALDYGSALFNRIE
                     SVEIKTENQLLFETAGTPYAKRIKGIVAIYERVAKAVSETLKNKDFPIVLAGDHSTAG
                     GTVAGIKMTYPKSKLGVIWIDAHADIHSPYTTPTGNVHGMPMAANLGEDNLANKINKP
                     DKETVDLWTKLKKIGG"
BASE COUNT         1031 a          582 c          645 g         1045 t
ORIGIN      
        1 ggtttgtatt gcagctgcaa atgccggtgc cacttcacaa gatttaaaaa caggttatat
       61 tgttggagca acaccacgtt atcagcaaat ggctttattc attggtgcta ttgtttcgtc
      121 agtagttata ggtttcactg taaaatatct tgacacgcct actgccgacc tgactgcaca
      181 aggtattcat catgccattg gtgaaaaata tgcagcaccg caggcaacac tcatggcaac
      241 attaatcaaa ggacttttga gttataatct cgactggcaa tttgtgcttg ttggcgtatt
      301 tattgcaata actatggagt tgtgtggtgt taaagctttg tcgtttgccg ttggagctta
      361 tttacctttg agtacaacct tgccgatttt tgcaggtggt atggtaaaag gtattgtaga
      421 ttggaatgca aaacgtaaac ataaaaaggc cgaaggtgat gatgagttag gcaaaggaaa
      481 tttgtttgct acaggtttgg ttgccggtgg tgcattggca ggcgtggtag tagcattgct
      541 ttctgttgat gaaggcgtgt atgccgctct tgaaaaagtt aatatggaac atagtattgc
      601 tgctgtaatc ggtgaagacg gttatcagtt attaggagta ggattcttta cctttatgtc
      661 tgtaatactt taccgcattg caattaaacc cgaaaaaaag acgttgaata attagtataa
      721 atccattaaa gatgaaaagg aatttaatag gtgttgtatt tagttttatt atcctgacag
      781 gatatgtttc ttgctcgcaa ccttcaaaaa ttgaacataa aataaaatgg atggactttg
      841 agtctgccgt tgctgcctct caaaaaaatc ctaaaaagtt atttattgat gtttatacag
      901 actggtgcgg atggtgtaaa aaaatggatg catcaacatt catggatgac tccgttgcag
      961 catttataaa tgcaaactat tatccggtta agtttaatgc tgaaagtaag gatactatac
     1021 acttttttga tcaggtattt gtttttgttc ctgaatataa aagtcatgag ctggcaattt
     1081 cattgcttaa tcgtcaaatg ggttatccat cgtatgcatt tcttgacgaa aaatttgctg
     1141 ctataaaagt acagcaaggc tatatgcctg tagaaacttt aatgcctttg ttgaagtact
     1201 atgcacatga tgcctacaaa acaaaaacgt ttgaggagtt tacgaaatag ttaggctgag
     1261 ttaattttaa taaacgaaat tgattttttt gaatttcgga aattaatacc ctaatatttt
     1321 catcatagat tttgagcttt gctcttttgc aaaaagccac atgctcaatt caccctcctc
     1381 agttttatct attaaaacat gttttggtgc aggcaatagg caatgctgaa taccgccata
     1441 tccaccgagt gaatcctgat aggcaccggt gtgaaagaag ccaagatata atggttcgtc
     1501 aggtgtaggt cgtggcataa aaacacgatt ttttaccgac tccataatat aataatcttc
     1561 accatcacaa gttatgccac ctaagtttac acgttgttgt tccttatccc agtggttgat
     1621 ggcaagcaga ataaattttt gtttgatacc ccatgtgtca ggtagtgtag tgataaaact
     1681 actgtcaatc atataccaca actctttgtc gttttgttgt ttaatgcctg tgacagaata
     1741 taaaattgca ccactttcac caacagtaaa actgccaaac tcagtaaata aatgtggtac
     1801 cggcactttg cgctgatcgc atgccgattt tatctgcatt acaatctcgt cagccatata
     1861 ttgatagtcg aaattaaatt caagtttcgt tttaattgga aacccaccac caatatcaag
     1921 agctgtcaac tcaggacaaa tctttttaag gtctgcatag aatgcaatgt ttcgctgaag
     1981 ttcattccag taatatgcag tatcacgaat gcctgtattg ataaaaaaat gaagaagctt
     2041 tagttcaaca ttagggttct tcttgatggt attcttgtaa aagtcaacaa tgtcacgttg
     2101 cctgatacct aaacgtgaag tgtaaaactc tgcattaggc tcttcttcag aagcaattct
     2161 gattccgatt ttaaacttct tgccgggcat tgccttctcg tattcctgaa gttcatcaac
     2221 attatccaga attggaatca cattggtaaa accatcttta ataagattaa caataccttc
     2281 aatataacct tcgcgtttaa aaccattgca aagaatataa atgtccttgt ttattttctg
     2341 cgactcatag agcgacctga taataggaat atcaaaagcc gaacttgttt caatatgaat
     2401 atcgtttttc agtgcttcct ccatcacgaa ctgaaagtgc gaactttttg tgcaataaca
     2461 gtagtaataa cttcctttat agtcaacttt tgccatagca actttaaaaa gtcgtttggc
     2521 tttttgaatt tgtgagctaa tcttaggcag ataggttatt ttcaacggtg tgccatattg
     2581 ttttatcaca tccatcaggt ttataccatt aaaatggagt gaatcgtcaa taacttcaaa
     2641 accttcttgt ggaaagtcgt aactttgttg aatcaggtca atgtaacggt tcttcatttg
     2701 caatacgaaa ggcttatttt tgtgccttgc gaatgtaaaa ataacctgaa taaaaaataa
     2761 cgatgttaaa aaatttatgg caagtaaaat aaaattcatt gaagttaaat cagaaattgg
     2821 tgccggtacc agaggtgcaa gccttggtgt tgatgcaata aaaattgcag ccttagacta
     2881 tggcagtgca cttttcaaca ggatagaaag tgtagaaatc aagactgaaa accaattact
     2941 cttcgagaca gcgggtactc cttatgcaaa acgtataaaa ggcattgttg ctatttatga
     3001 acgtgtggca aaggccgttt ctgaaacatt aaaaaataaa gatttcccga ttgtgttagc
     3061 cggagaccat agcactgccg gtggtaccgt tgcaggcatt aaaatgactt atccaaagtc
     3121 gaagctgggt gtaatctgga ttgatgccca tgccgacatt cactctccat atactacccc
     3181 aacaggaaat gttcatggta tgcctatggc agcaaatctt ggtgaggaca atcttgcaaa
     3241 caaaatcaac aaacccgata aagaaactgt tgacctttgg acaaagttga agaagatagg
     3301 agg
//
LOCUS       DAOOOL010000214         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3436_length_3296_cov_44.509411, whole genome shotgun sequence.
ACCESSION   DAOOOL010000214 DAOOOL010000000
VERSION     DAOOOL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_3296_cov_44.509411"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..722
                     /locus_tag="PKU82_11670"
     CDS             300..722
                     /locus_tag="PKU82_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ83563.1"
                     /translation="MRQKLNLITLGVKDFQKSLDFYEKGLGWKRSDKSMDGLALFQLG
                     GIVLALHPLKDLADDATLTYHSSEFYGLTISHNTHSEKEVDFIMKQVASIGGTVVKPA
                     QKVYWGGYSGYFKDLDGYLFEVAFNPFWDLDNNDNLLL"
     gene            758..1450
                     /locus_tag="PKU82_11675"
     CDS             758..1450
                     /locus_tag="PKU82_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMUG2 DNA glycosylase family protein"
                     /protein_id="HOZ83564.1"
                     /translation="MKNTFADRVITFNKSLIYSGKLPPGFDVLNPFNDNPETTIVMQQ
                     FYKEFYNDNKKRKFIIGINPSRHGAGVTGVPFTDTKRLESVCKIKMQSAHTHEVSSVF
                     MYDMIQSYGGAKSFYSDFYINSPFPLAIVRKKNRNWLNANYYDDATLFLMLKDYMIET
                     LKKHINLGLITSEVFILGKKNALFISEINKKANLFKKMTVLDHPRFIQQYKSKEKLNY
                     INKYLNAFRSAV"
     gene            complement(1452..>3296)
                     /locus_tag="PKU82_11680"
     CDS             complement(1452..>3296)
                     /locus_tag="PKU82_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOZ83565.1"
                     /translation="IPASQCLETVRTLLKMGTKAFIVFSAGFSEAGERGIELEKELTD
                     MVNKAGGTLIGPNCVGIISEKYKGVFTTPVPEYDPQGCELISSSGATAVFIMESALLT
                     GLRFSNVYSIGNAAQTGVEEILEYLDVNFDEHKSPKVQLLYLEQVKKPFKFLKHTTSL
                     IQKGCRIAAIKSGYSEAGGRAASSHTGALATADNVIRALFRKAGIVYCSSRDELIAVG
                     SVFQSKELKGENIAIITHAGGSAVMLTDALTSNGLNVPQFDSDKAATLLSKLNPGSSV
                     NNPIDFLATGTAKHLGEIIDFCEGYDVVDAMVVVFGSPGLFNVKDVYELLDKKMSACT
                     KPIYPVLPSLINAEKEIKQFLSKRRVNFPDEVNLGKALAHVYTSTKYRFEKTTLPEMD
                     IVSVRSIINTAQNGYLDAQTNQELLAAAGIAQAEQLIISDKSQFEKVNSKIKFPVAMK
                     VIGPVHKTEVNGVMLNINSIEQALNAFEILMKIPDASAVLVQEMVKGEELYCGAVRQG
                     DFGHVVLCGLGGVFLELLQDTASALAPMSYGEVKSMVESLKAYPLFKGYRNKKGLNEE
                     KFIEIVMRIGALVHIAPEISELDLNPIKADEHNMIVVDSRICIDKNGR"
BASE COUNT         1080 a          647 c          510 g         1059 t
ORIGIN      
        1 ttcttcatta tctgatgctg ctattctggc atcatcatca gcagttactt gttgtttaat
       61 ttcagcctga ccacctgcaa acgaaaaact aacaaaacaa agtagtggaa gtattgaata
      121 aagtatgcgg aactttctca tgattataac ttttaagttt aatatatgac gcccataacg
      181 aacaaaaagt tacccaaatg tagtatattt tccaattgtt taaagaaatt tctaaaaatt
      241 tttaaaagta tcagtaagtt tgacccataa gtgaataatt aattataaag ttaaatatta
      301 tgcgtcagaa attaaatctg ataactttag gagttaaaga ttttcaaaaa tcacttgact
      361 tttatgaaaa aggtcttggc tggaaaaggt cagacaaaag tatggatggc cttgcattgt
      421 ttcaacttgg cggtattgtt ctggcactac atccactgaa agaccttgct gatgatgcaa
      481 cacttacgta tcactcatcg gaattttatg ggctaactat atcgcacaat acacattctg
      541 aaaaagaagt agatttcata atgaaacagg ttgcttcaat tggtggcaca gttgttaaac
      601 cggcacaaaa agtatattgg ggtggctata gtggttattt taaagattta gatggctatc
      661 tttttgaagt tgccttcaat cctttttggg atttggataa caatgataat ttgcttttat
      721 aatcttctat ccaacgctat tttaaaaatt acaattagtg aaaaatactt ttgcagacag
      781 ggtaataacc ttcaataaaa gtttaatcta ttcaggtaaa ttgcctccgg gttttgatgt
      841 ccttaatcct tttaatgaca atccggaaac tactattgtc atgcagcagt tttataaaga
      901 attttataac gacaataaaa agcgaaaatt cattatcggc ataaatccaa gcaggcatgg
      961 tgcaggtgtt accggagtac cattcacaga cacaaaacgt ttggaaagtg tttgtaaaat
     1021 aaaaatgcaa tcagcacaca cgcatgaagt ttcttctgta tttatgtacg atatgattca
     1081 atcttacggt ggtgcaaaat cgttctatag tgatttttat atcaactcgc cttttccatt
     1141 agctatcgtt cgcaagaaaa atcgcaactg gcttaatgca aattactatg atgatgcaac
     1201 tctgtttcta atgcttaaag attacatgat agaaacttta aaaaaacata tcaatttggg
     1261 tttaattacc tctgaagttt ttattttagg taagaaaaat gctttattca tcagtgaaat
     1321 aaacaaaaaa gctaatctgt ttaaaaaaat gacagtctta gatcatccaa ggtttataca
     1381 acaatataaa tcgaaagaaa aattaaatta catcaataaa tatttaaatg catttagaag
     1441 tgctgtataa actatcttcc gtttttgtca atgcatattc ttgaatcaac aactatcatg
     1501 ttatgctcat ctgcttttat tggattcaaa tccagttcgc ttatttcagg agcaatatgt
     1561 acaagtgcac caatacgcat cacaatttca ataaactttt cttcattcaa acctttttta
     1621 tttctatagc ctttgaaaag cggataggct tttaaagatt caaccattga ttttacttca
     1681 ccataagaca ttggtgccaa tgcagaagct gtatcctgaa gcaattctaa aaacacacca
     1741 ccaagtccgc acaaaactac atgtccaaaa tctccttgtc ttacagcacc acaatataac
     1801 tcctcaccct taaccatctc ctgaaccaaa actgcagatg catctggaat tttcatgagt
     1861 atttcaaatg catttaatgc ttgttcaatg ctattaatat ttaacatcac accattgact
     1921 tctgttttat gcacaggtcc tatcactttc atggcaacag gaaattttat cttgctattg
     1981 actttctcaa actgactctt gtctgaaata attaactgtt cagcctgtgc tataccggct
     2041 gcagcaagta actcctgatt tgtttgagca tcaagatagc cattttgggc ggtattgatg
     2101 atagaacgaa cggacacaat atccatctca ggcaaagtgg ttttttcaaa acgatatttt
     2161 gtagatgtat atacatgagc cagtgctttt ccaagattta cctcatcagg aaaattaact
     2221 ctgcgttttg acaaaaactg cttaatctcc ttctccgcat taatcaacga tggcaataca
     2281 ggataaattg gttttgtaca agcactcatt tttttatcga gtaactcata cacatctttt
     2341 acattaaaca aaccggggct gccaaaaact acaaccatag catcaacaac atcatagcct
     2401 tcacaaaaat caataatctc tccaagatgt tttgctgttc ctgttgctaa aaaatctatt
     2461 ggattattta cagacgatcc cggattaagt tttgacaaca atgttgctgc cttatcagaa
     2521 tcaaattgcg gtacattcaa gccgtttgaa gttagtgcat cggtaagcat tacagcagaa
     2581 cctcccgcat gggtgataat tgcaatattt tctcctttta attccttaga ctgaaaaaca
     2641 cttcctactg ctattaactc atctctacta ctgcaataca caatacctgc tttacgaaac
     2701 aatgcacgga taacattatc tgccgtggct aatgcaccgg tatgcgatga tgcagctctg
     2761 cctcctgctt ctgaatagcc tgattttatt gcagctatcc gacatccctt ctgaatgagc
     2821 gatgttgtat gttttaaaaa cttaaacggc ttttttacct gctcaagata aagcagctga
     2881 acctttggac tcttatgctc atcaaaattt acatcaagat attcaagaat ttcttcaaca
     2941 cctgtctgtg ctgcattgcc aattgaataa acattagaaa accttaatcc ggtaagtaac
     3001 gcagactcca taataaacac tgcagttgca ccggaacttg aaattaactc gcatccctgt
     3061 ggatcatact ccggtacagg agtagtgaaa acacccttat atttttcact aataatacca
     3121 acgcaattgg gtcctattaa agtgcctcct gctttattca ccatatcagt aagctctttt
     3181 tcgagttcta ttccacgctc gcccgcctca gaaaaaccag ctgaaaaaac aataaatgct
     3241 ttagtaccca tctttagcaa agtcctgact gtttccaagc attgacttgc aggtat
//
LOCUS       DAOOOL010000215         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3457_length_3284_cov_38.567049, whole genome shotgun sequence.
ACCESSION   DAOOOL010000215 DAOOOL010000000
VERSION     DAOOOL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3457_length_3284_cov_38.567049"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKU82_11685"
     CDS             <1..900
                     /locus_tag="PKU82_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOZ83566.1"
                     /translation="GRNKILKFEGCYHGHVDSMLVKAGSGLVTFGTSSSAGVPESFVN
                     DTIVVPLNNIDAVKTAFEEFKNQIAAVIIEPVPANNGLLLQTREYLQQLRNICTENKT
                     LLHFDEVISGFRLGFEGAAGYYGIQPDIITYGKIIGGGLPVGMYGASKEIMAKISPDG
                     DVYQAGTLSGNPVAMAAGIAQLTECLKTDFYSLLEKKTKKLCTAVEAHAHSKGYTFRM
                     FNIASIFWIAFTDAESIRSADEIDAASMSKFRVLHAELLESGIYLGPSGYEVGFISEA
                     HSDADIDRTIVAMCKALDKVFPQ"
     gene            908..2437
                     /locus_tag="PKU82_11690"
     CDS             908..2437
                     /locus_tag="PKU82_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOZ83567.1"
                     /translation="MQEYDVVIIGSGLGGLLCANILAEEGFKVCVLEKNQQIGGNLQT
                     FVRNRVIFDTGVHYLGGLAEGENLNRFFRYFGIMDKLKLEKMDENGFDIVTFDGDKNE
                     YPYAQGYENFIRVLSEKFPDEKKAIQKYCDEIKRICSRFPLYNLQNGESYHSDSELLQ
                     TNAKAFIASITTNEKLRNVLAGTNLLYAGIGEKTPFYLHALVLNSYIESAWRCVGGGS
                     QIAKLIEKNIRKFGGDIFKRSEVKQLKFEGDDLRYAELTNGKTIKAKRFISNAHPSVT
                     LDLLEPGKIRKSYHTRFKSLQNSASAFTLYLVFKENTVNYINHNYYHFKNNDVWAPVD
                     YKPGEWPLSYGTFMVAGLKPDKYAEGMAVLTYMKYDEVKQWENTASTVAHPDFRGDDY
                     EEFKRKKSEILIDELEKKFPGIRENIKAYYSSTPLTYRDYIASPGGSMYGIVKDSFDP
                     LKTLVPARTKVKNLFFTGQNLHFHGVLGVTVSAFVTCSEILGLDYLLEKIAKKEATSN
                     K"
     gene            2441..3049
                     /locus_tag="PKU82_11695"
     CDS             2441..3049
                     /locus_tag="PKU82_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOZ83568.1"
                     /translation="MPVFLDGFLVGLALVILIGPVFFTLLKTSLQHGVVAGSLVALGI
                     FISDILAVLLCVYGASAFLKSDESRFYLALVGGVVLIALGVRYFLKKKKAEEEAIQLN
                     TKHYIGFFSKGFLVNFVNPFVFAVWIGIIKVAGDKHGFEKDLGIYLAGTLLAIFSTDM
                     TKVFLAAKIKNILKPLILFVLYKIIGVCLILLGLRLFYVAFS"
     gene            complement(3046..>3284)
                     /locus_tag="PKU82_11700"
     CDS             complement(3046..>3284)
                     /locus_tag="PKU82_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOZ83569.1"
                     /translation="TGIVIACMVDVSRLAIYGSNHFGITQNEISIVAAGTLSAFAGAY
                     LGNKLVKKVTLKSIQLFVTVMLIVFAIALGAGII"
BASE COUNT          986 a          597 c          714 g          987 t
ORIGIN      
        1 ggcagaaaca aaattttaaa attcgaaggt tgttatcacg gacatgttga ttcgatgctg
       61 gtgaaagcag gttcgggatt ggttaccttc ggaacaagtt cttctgccgg tgttcctgaa
      121 tcatttgtga acgatactat tgttgttcct cttaataata ttgatgcggt aaaaacagca
      181 tttgaagaat ttaaaaatca gattgcggct gtaattattg aacctgttcc tgctaataac
      241 ggtttgttgt tacaaaccag ggaatacctg caacaactca gaaacatctg caccgaaaac
      301 aaaacgctgc tgcactttga tgaagtgatt tccggcttcc ggcttggctt tgagggtgct
      361 gcaggctact acggaattca gcccgacatt attacgtatg gaaaaataat cggtggaggt
      421 ttgccggtag gaatgtatgg ggcaagcaaa gagattatgg ctaaaatttc tcccgatggt
      481 gatgtttatc aggcaggaac attatcggga aaccctgttg ccatggctgc cggcattgca
      541 cagctgacag aatgcctgaa gacagatttc tattctttac ttgaaaagaa aacaaaaaaa
      601 ctttgcacag ctgttgaagc tcatgctcat tcaaaaggct atacattcag aatgttcaac
      661 atcgcttcca ttttctggat tgcatttaca gatgcagaaa gtattagaag cgcggatgaa
      721 attgatgcag caagcatgag taaattcaga gtgcttcatg ctgaattatt ggagagcgga
      781 atttacctcg gaccttccgg ttatgaggtt ggttttattt ctgaagcgca ttctgatgca
      841 gacattgaca gaacaattgt tgcaatgtgt aaggctttag acaaggtttt cccgcaataa
      901 aattattatg caggaatatg atgtggtgat aatcggaagc gggctgggtg gcttgctctg
      961 cgccaatatt cttgctgaag aaggctttaa agtttgtgtg cttgaaaaga accagcagat
     1021 tggcggcaac ctgcaaacct ttgtccgcaa ccgcgtgatt tttgataccg gtgttcacta
     1081 cctcggtggt ctggctgaag gcgaaaacct gaatcgtttc ttcaggtatt tcggcattat
     1141 ggataagctg aaattagaga agatggatga gaacggtttt gatattgtta cgtttgacgg
     1201 agataaaaac gagtatccgt atgcacaggg atatgaaaat ttcattcgtg ttctgtctga
     1261 aaaattcccg gatgagaaaa aagcaattca gaaatattgc gatgaaataa aaaggatttg
     1321 cagtcgcttt cccttatata atctgcaaaa tggcgaaagt tatcattctg attcggaatt
     1381 attgcaaacc aatgccaaag catttattgc atcaattaca actaatgaaa aactcagaaa
     1441 tgtattggca ggcactaatt tattgtatgc aggaataggg gagaagacac ccttttatct
     1501 tcatgcgttg gtgctgaaca gttacattga aagtgcatgg cgttgcgttg gaggcggttc
     1561 gcagattgcc aaactgattg aaaaaaatat cagaaagttt ggcggagata tttttaaacg
     1621 cagcgaagta aaacaactga aatttgaagg agatgatttg cgttatgccg aactcacaaa
     1681 cgggaaaaca ataaaagcaa aacgatttat ttcaaatgct catccttcgg taacgcttga
     1741 tttacttgag cccggcaaga tcagaaagtc ctatcacacg cggtttaaat cgttacaaaa
     1801 ttctgcttcg gcatttacgc tgtatctcgt ttttaaagaa aatacggtca actatattaa
     1861 ccacaattac tatcatttta agaataacga tgtgtgggca cctgttgatt acaaacccgg
     1921 agaatggccg ttgagttacg gaacatttat ggttgccggc ttaaaacctg ataaatacgc
     1981 agaaggtatg gctgttctta cttacatgaa atacgatgaa gtgaagcaat gggagaatac
     2041 cgccagcaca gttgcacacc ctgattttcg cggagatgat tatgaagaat tcaaacgcaa
     2101 aaaatcagaa atacttattg atgaactgga gaaaaagttc cccggaatac gtgagaatat
     2161 aaaagcatat tatagctcaa ctccccttac gtaccgtgat tacattgctt ctcccggtgg
     2221 ttccatgtat ggaatagtaa aggattcatt tgacccctta aaaacacttg tccctgccag
     2281 aacaaaagtt aaaaatcttt tttttaccgg acagaatttg cattttcacg gagtgttagg
     2341 tgtaactgta agtgcctttg ttacctgttc cgaaatactt ggattagatt atttgttaga
     2401 aaaaattgca aagaaagaag ccacatcaaa taaatagaag atgcctgttt tcttagatgg
     2461 ttttttagtc ggacttgccc ttgtaatcct tatcgggcct gtgtttttta cactgctgaa
     2521 aacatctttg caacacggtg ttgttgcagg cagcttagtt gctttaggaa tttttatcag
     2581 cgatatactt gctgtattgc tttgtgttta tggtgcttca gcatttttaa aaagtgatga
     2641 gagccgattt taccttgctc ttgttggcgg agtagttctg attgctctcg gtgtccgtta
     2701 ttttttgaaa aagaaaaaag ccgaagaaga agcaattcag ttgaacacaa aacactacat
     2761 cggttttttc agcaagggct ttcttgtcaa ttttgtaaat ccctttgtgt ttgcagtgtg
     2821 gataggcatt ataaaagtag caggcgacaa acacggattt gaaaaagacc ttggcattta
     2881 ccttgcagga accttgctcg ccattttcag tacggatatg accaaggttt ttcttgcagc
     2941 caaaatcaaa aacattttaa agccgctgat actttttgtg ctttacaaaa taattggtgt
     3001 ttgccttatc ttgctgggac tgcgtttgtt ctatgttgct ttcagttaaa taattcctgc
     3061 gcctaatgca attgcaaaca ctatcagcat caccgtaaca aacagttgta tcgatttcag
     3121 cgttactttt tttaccagtt tgttgcccag ataagcacct gcaaatgcac ttagtgttcc
     3181 tgctgcaaca atagaaattt cgttttgtgt aatgccgaaa tgattgcttc cgtatatagc
     3241 aagacgcgat acatccacca tgcaggcaat cacaattccg gtgg
//
LOCUS       DAOOOL010000216         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3476_length_3275_cov_23.730124, whole genome shotgun sequence.
ACCESSION   DAOOOL010000216 DAOOOL010000000
VERSION     DAOOOL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3476_length_3275_cov_23.730124"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKU82_11705"
     CDS             complement(<1..257)
                     /locus_tag="PKU82_11705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ83570.1"
                     /translation="MALSQTDKSQAKLQAEKSQNKETMVEITTNLGTMRFKLYNETPL
                     HRDNFIKLANEGFFDSTLFHRVIKNFMIQGGDPQSKNAAAGA"
     gene            387..752
                     /locus_tag="PKU82_11710"
     CDS             387..752
                     /locus_tag="PKU82_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83571.1"
                     /translation="MKSSLRKTHLVLTSLIVMTVLFTFSCKKEGPCEAIITITDSAGV
                     AVRGATVILRQDAVVNPTTGVQANVYQEGVTDGYGKASFEFKLEAVLNIEASKGPKLG
                     KDYVRLEQSKTVYRTVIIK"
     gene            complement(745..1119)
                     /locus_tag="PKU82_11715"
     CDS             complement(745..1119)
                     /locus_tag="PKU82_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83572.1"
                     /translation="MNETNIQKIKHKWGELLRQLEITFGEKPDLQGMIFLIGVQELGK
                     GYQNFTKEEKQDLMHIATCKLLSYDGYYKFTGVDSDGWPQYEKAKTMPQMPLKEQDLL
                     LKINIIRYFEEDGYELEKSEVI"
     gene            1259..2350
                     /locus_tag="PKU82_11720"
     CDS             1259..2350
                     /locus_tag="PKU82_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83573.1"
                     /translation="MAWLLPLAEVSAKNQPVDIVFTLDLSGSTNGLIDDVRDNIWGMN
                     NELTRLYPNADIRFAVVGYSRPSFGSKNQFVKVISPFTSNIDFIATELYKLKPNIEKG
                     DQYVGAAIRASLDMLSWSHEKNAVKQIFLTGNGSVFLGAFDVVESCDLAKEKGITVNS
                     LYCYSSLRAKEISGWYKISEITGGKSIDVKVHKRLPDYATVTDFNRLQMLATELNKTY
                     IYYGKEGRDKFKAMVSNEKNVLNARHSAFEDLLYHKISDRFQGKQSDWDLVDYLKSRN
                     GNLKNVDARLLPDSLKNINPEQLLTKLMILKERRSYLLSQIRQLLPFDRQDKLTAYFN
                     SKQSDSDMIFDRQVMMVVKDEMQSDLAAN"
     gene            complement(2354..3064)
                     /locus_tag="PKU82_11725"
     CDS             complement(2354..3064)
                     /locus_tag="PKU82_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOZ83574.1"
                     /translation="MKNRRDFIKTSALAAGTLALYGIGSKVAIASETNEDNLFKLPDL
                     EYGFEALEPYIDTLTMEIHYTKHHQGYVNNLNKAVVAEKLQGVSLNDLLGKVSKHSVA
                     IRNNAGGHYNHSFFWRLMRPGGAKSPKGELAEAIIRELSGTDNLQKAFSDAAKNRFGS
                     GWAWLIKSADGKLSVTSTPNQDNTLMDIAEVKGKPILGIDVWEHAYYLHYQNKRADYI
                     NAWWNLINWDVALENYNS"
     gene            complement(3076..>3275)
                     /locus_tag="PKU82_11730"
     CDS             complement(3076..>3275)
                     /locus_tag="PKU82_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOZ83575.1"
                     /translation="APMPGLVLDILVSEGQAIQKGDSLLILEAMKMENNLKATNDAVV
                     KKIKVLKGDKVEKNTVLIELS"
BASE COUNT          996 a          594 c          580 g         1105 t
ORIGIN      
        1 gcaccggcag cagcattctt agactgcggg tcgccaccct gaatcataaa atttttgatg
       61 acacgatgaa acaacgtgct gtcgaaaaaa ccttcattgg caagtttaat gaagttgtcg
      121 cggtgtaatg gcgtttcatt gtagagttta aatcgcattg tacctaaatt ggttgtaatt
      181 tccaccatag tttctttgtt ttgacttttt tccgcctgaa gttttgcctg gcttttatcc
      241 gtttgtgaca gtgccatcag gggaacgatt gttgcaataa tacagatgat ttttttcatt
      301 tctattttta attacttttg agctgataaa acattttacg ataccgttcg taaaaacagg
      361 tacaagaata ataaatttcc attcgcatga aaagtagttt acgtaaaact catttagttt
      421 taacttcatt gatagtaatg acagttcttt ttactttttc ctgtaaaaaa gaaggacctt
      481 gcgaagctat cattaccatt actgatagtg caggagtagc cgttagaggt gcaactgtaa
      541 ttttaagaca ggatgctgtt gtaaacccaa ctacaggggt gcaggctaat gtctatcagg
      601 aaggtgttac tgatggttat ggtaaagcct cttttgagtt taagttggag gcagttttaa
      661 atattgaagc cagtaaaggc cccaaactcg gcaaagacta tgtacgtttg gagcaatcaa
      721 aaactgttta ccgtacggta atcatcaaat aacctcgctt ttttcaagtt catatccatc
      781 ttcctcaaaa tagcggataa tgttgatttt cagcaataaa tcttgctctt ttaaaggcat
      841 ttgaggcatt gttttagctt tttcatactg tggccagcca tcggaatcaa cacctgtaaa
      901 cttataataa ccatcatagc taaggagttt gcaagtggct atatgcatca aatcctgttt
      961 ttcttcctta gtaaagttct gatatccttt tcccagttct tgtactccaa ttaggaaaat
     1021 cataccctgt aaatcgggct tttcaccaaa ggttatctcc aattgccgta aaagttcacc
     1081 ccacttatgc ttgattttct gaatatttgt ttcgttcata aacaataaag tgttcaaaag
     1141 taccaatatt agagactaaa ccaccaaaca ataaaatatc taattttgat aaaccacaat
     1201 aaatccacaa taaacaatga acctgttaag attaaaactg gcaattatag ccgtctttgt
     1261 ggcttggttg ctacctctgg ctgaggtttc agctaagaat caacctgttg acattgtctt
     1321 tacacttgac cttagtggaa gcaccaatgg gctgatagat gatgttcgcg ataacatctg
     1381 gggaatgaat aatgaactta cccgacttta tcctaacgct gatattcgtt ttgcagttgt
     1441 tggatattca cgtccaagtt ttggcagcaa aaatcagttc gttaaagtta tttctccatt
     1501 tacatcaaac attgacttta tagcaacaga actttacaaa ctgaagccta atattgaaaa
     1561 aggcgatcag tatgttggtg ctgcaatacg tgcttcgctt gatatgttga gttggtcgca
     1621 tgaaaaaaat gcagtgaaac aaatttttct gacaggaaat ggttcagtgt ttttaggcgc
     1681 atttgatgtt gttgaatctt gtgatctggc caaagaaaaa ggaataactg taaattcatt
     1741 gtattgctac agttcattac gtgccaaaga aattagtggc tggtataaaa tttctgaaat
     1801 taccggtgga aagtcaattg atgttaaagt gcataaacgt cttcctgact atgcaacagt
     1861 aacagatttt aaccgtcttc agatgctggc aacagaactc aataaaactt atatttatta
     1921 tggtaaagaa ggccgtgata aatttaaggc tatggtaagt aatgagaaga atgtgcttaa
     1981 tgcacgtcat tctgcatttg aagatttgtt atatcataaa atttccgatc gctttcaggg
     2041 aaaacagagt gattgggatt tggttgatta tttgaaatca agaaacggta atcttaaaaa
     2101 tgtagatgct cgtttacttc cggactcatt aaaaaatatt aatcccgaac agttgcttac
     2161 taagctaatg attttgaaag aaagaagaag ttatttgtta agtcaaatac gtcagttact
     2221 tcctttcgac cgtcaggata aattaacagc atattttaat tcaaagcaat cggacagcga
     2281 tatgattttt gaccgtcagg tgatgatggt tgtaaaagat gaaatgcagt ctgatttagc
     2341 tgcaaactga tgctcaggaa ttataatttt caagtgctac atcccagttt atcaaattcc
     2401 accaggcatt aatataatct gctcttttat tctgatagtg cagatagtat gcatgttccc
     2461 aaacatcaat tcctaatata ggtttacctt taacttcggc aatatccatc agtgtgttgt
     2521 cctgatttgg agtagatgta acgcttagtt tgccgtcagc tgatttaatt aaccaagccc
     2581 aaccggaacc aaatctgttt tttgcagcat ctgaaaatgc tttttgtaaa ttatcggtac
     2641 cacttaattc tctaattata gcctcagcta attctccctt tggtgatttt gctccaccgg
     2701 gacgcatcaa cctccagaaa aaagaatgat tgtaatgtcc acctgcatta ttgcgaattg
     2761 caacagaatg ttttgatact ttacctaaca aatcatttaa agacacacct tgtaattttt
     2821 ctgcaacaac tgctttgtta agattgttta catatccctg atgatgcttt gtataatgta
     2881 tttccattgt caatgtatca atatatggct ctaatgcttc aaaaccatat tccaaatcgg
     2941 gcaatttaaa taagttgtct tcgttggttt ctgatgcaat ggcaaccttg ctgccaattc
     3001 cgtacaatgc cagcgttcct gcagccaatg ccgaagtttt tataaaatct ctcctgtttt
     3061 tcattttctt tataattaag ataattcaat caatactgta ttcttttcaa ctttatcacc
     3121 ctttaatact tttatttttt ttacaacagc atcattggta gctttaagat tgttttccat
     3181 tttcatagct tccaatatta gtaggctatc gcctttttga atagcttgtc cttcggaaac
     3241 taaaatatca agtaccaaac cgggcatagg tgctt
//
LOCUS       DAOOOL010000217         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3489_length_3263_cov_75.054863, whole genome shotgun sequence.
ACCESSION   DAOOOL010000217 DAOOOL010000000
VERSION     DAOOOL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3489_length_3263_cov_75.054863"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PKU82_11735"
     CDS             complement(<1..904)
                     /locus_tag="PKU82_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ83576.1"
                     /translation="MKLKVIFTILIVGLLSVLACKKDKDDDPINNLPPYNPTPYTINT
                     PSNLPAMNVPSFNPTTVEGVYLGRKLYYDKLLHPTGTSSCSSCHIQSQGFAVTGTNII
                     PHVNLGYQNKFLWNGEIQGTLEDAMMFEVETFFNTDISRLQKDSLYPILFYKAFGSTT
                     IDTKKCAYALAQFLRTVVSGNSKLDRFIRHETLFTADEIQGFNIFFSEKGDCFHCHSL
                     SLTSDGDLHNIGLDSVFTGVNAGYYNVSHNAADYGKFRTPSLRNTALKTTFMHDARFT
                     TLDQVIEHYNTGVKHSPSLDPVMTK"
     gene            complement(906..1631)
                     /locus_tag="PKU82_11740"
     CDS             complement(906..1631)
                     /locus_tag="PKU82_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83577.1"
                     /translation="MRTSILLIRNVILIALMTIMVSSCKKKDDDENKLNLNLSFAVDG
                     SKLEFNSIKYVNAAVNNYSVSKLHFYISAFEFENTDGNKFSFDSIFYVDAATQAPSFT
                     LEGLPVGNYKSLRFLIGLDSLHNISHALPNTIDNENMSWPDMMGGGYHFMKLEGNFMD
                     GGNPTGYAMHLGKNVHVVQVNIQKDFKVSGGTAALNLEMNINEWFSNPHNYDFNIDGN
                     YSMSDSVAMSKLALNGKDVFNIQ"
     gene            complement(1671..1940)
                     /locus_tag="PKU82_11745"
     CDS             complement(1671..1940)
                     /locus_tag="PKU82_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOZ83578.1"
                     /translation="MNDDAFKGKMLYQKFNCTACHQLYGLGGYLGPELTSAYSQKAQN
                     DAYFSAILKNGTVRMPDFNLSDKEVNQVIEYLKYIDQTSKHISAY"
     gene            complement(2230..2745)
                     /gene="paaJ"
                     /locus_tag="PKU82_11750"
     CDS             complement(2230..2745)
                     /gene="paaJ"
                     /locus_tag="PKU82_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOZ83579.1"
                     /translation="MVNDSLKEQSSLESSEVFQLLYAIPDPEVPVITIGELGVLRNVE
                     MINGRIVIYITPTYSGCPAMFAIESEIITILKENNISDFKVKTVHFPAWTTDWLTDAA
                     KKKLSDYGIAPPEHSSQIKHLRPDEVSLKCPFCQSDETHLTSWFGSTACKALYYCDNC
                     RQPFEHFKCHH"
     gene            complement(2739..>3263)
                     /gene="paaC"
                     /locus_tag="PKU82_11755"
     CDS             complement(2739..>3263)
                     /gene="paaC"
                     /locus_tag="PKU82_11755"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HOZ83580.1"
                     /translation="QFRNLLLTEQPNVDFGFTIIRQLFVSTWQQYLYSELTHSTDKNI
                     SALAIKSLKEVNYHLRFASDWTLRLGDGTEESHQRVQNAVNELWNYTGDLFEIDQVDK
                     ELLKHGIAADMKKVEESWNKHIKQVLNDATLSIPEINNFMRTGSRKGVHTEHIGFILA
                     EMQFLPRAYPDAKW"
BASE COUNT         1056 a          585 c          549 g         1073 t
ORIGIN      
        1 gctttgtcat aaccggatct aaagatggtg aatgtttaac acctgtgttg taatgttcaa
       61 ttacttggtc aagtgtagtg aaacgtgcat cgtgcatgaa ggttgttttt aatgctgtat
      121 ttcttagcga tggtgtacgg aactttccat aatctgcagc attatgactg acattataat
      181 accctgcatt gacacctgta aaaactgaat cgagtccgat attgtgcaaa tcgccatcgg
      241 aagttaaaga caacgaatga cagtgaaaac agtcgccttt ttctgaaaag aaaatattaa
      301 atccttgtat ttcgtcagct gtaaacaaag tctcgtgacg gatgaaacga tctaacttag
      361 aattgccgga aacaacagtt ctcagaaatt gggccaatgc ataagcacac tttttagtat
      421 caatagttgt actgccgaat gctttataaa aaagaatagg atataagcta tctttctgaa
      481 gtcttgaaat atctgtatta aaaaaagttt ctacttcaaa catcatagca tcttctaatg
      541 ttccctgaat ttcgccattc cataaaaact tattttgata gcctaagtta acatgtggaa
      601 taatattcgt gcctgtaaca gcaaagcctt gcgactgaat atggcatgat gaacatgatg
      661 aagtacctgt aggatgtaaa agtttatcat agtataactt tcgccctaga taaacacctt
      721 caactgtagt agggttgaat gatggaacat tcattgccgg taaatttgaa ggtgtgttga
      781 ttgtatatgg tgttggattg taaggaggaa ggttatttat aggatcatca tccttatcct
      841 ttttacaggc aagtacagag agcaacccaa caatcaatat agtaaaaatt acttttagct
      901 tcatactatt ggatattaaa aacgtctttt ccgtttaatg ctagttttga catggcaacg
      961 ctgtcactca ttgaataatt tccatcaatg ttaaagtcgt agttatgcgg attagaaaac
     1021 cattcattaa tattcatttc cagattaagt gctgctgtac cgcctgaaac tttaaaatct
     1081 ttctgaatgt ttacttgcac cacatgcaca tttttcccta gatgcattgc atatccggtt
     1141 ggatttccac cgtccataaa attaccttcg agtttcataa aatgataccc acctcccatc
     1201 atatcaggcc aactcatatt ttcattgtca attgtatttg gcaaagcgtg gctgatgtta
     1261 tgcaaactgt cgagaccaat taaaaaacga agtgatttat aatttccaac aggcaatcct
     1321 tcaagcgtaa aagatggtgc ttgagttgca gcatcaacat aaaagatact atcaaaagaa
     1381 aacttatttc cgtcagtatt ctcaaactca aatgcagaaa tatagaaatg caatttcgaa
     1441 acactgtaat tgttgactgc agcatttaca tactttatgc tattaaattc aagtttacta
     1501 ccatccactg caaaagataa atttagattg agtttgtttt catcgtcatc ttttttctta
     1561 caagatgaaa ccattatggt catcaaggca atcaatatga catttcgtat taataatatt
     1621 gaagttctca tactttattg attttattgc ttgtcttcat gttcaggttt ttagtatgca
     1681 gaaatgtgtt ttgatgtttg atcaatgtat ttcaaatact cgattacctg attaacctct
     1741 ttatcactca aattaaaatc gggcatacgt acagttccat ttttgagtat tgcagaaaag
     1801 tatgcatcat tttgtgcttt ctgcgaatag gctgatgtaa gctctgggcc aagatatcca
     1861 cctaagccat aaagttgatg acatgcagta cagttgaatt tctgatagag cattttccct
     1921 ttaaacgcat catcgttcat tgtaaaagtg gcgcctgcag ttgccttagt gtaaacaaat
     1981 gcggtgtaaa aactaaataa tgtaaaaagt gtaataagta ccttaaactt taaactcact
     2041 tgcatagctg aatcgttttt gcatttccat aatcaaagaa atgctgaatc atcatataca
     2101 aatgaacaat tatcagcatg aaaacaaatt gactaatgac agtgacaagg ctgactttta
     2161 tcactgttta aaaaagtatc agaatagact gaaaaataaa tatttaaacg agcctgttaa
     2221 tagaaaatat taatgatggc atttaaaatg ttcaaatggt tgcctgcaat tatcacaata
     2281 ataaagagcc ttgcatgctg ttgagccaaa ccaactggta agatgtgtct catctgattg
     2341 acaaaaagga cattttaaac ttacctcatc aggacgcaaa tgttttattt gtgacgaatg
     2401 ttcaggtggc gcaatgccat aatcagaaag cttcttttta gcagcatcag taagccagtc
     2461 agtagtccat gcaggaaaat gtactgtctt tactttaaaa tctgagatgt tattttcttt
     2521 aagaatagtt ataatttctg attcaattgc aaacatagca gggcaacccg agtatgttgg
     2581 tgttatataa atcactatcc tgccgtttat catttcaaca ttgcgaagca cacccaattc
     2641 tccaatagta attaccggca cctcaggatc aggaatggca tacagtaact gaaaaacttc
     2701 tgatgattct aaactgcttt gttctttcaa ggaatcattt accatttggc atcaggatat
     2761 gcacgaggta aaaattgcat ttctgcaaga ataaatccta tatgttctgt atgtactcct
     2821 ttgcggcttc ctgttcgcat aaaattatta atctcgggaa ttgataacgt ggcatcattc
     2881 agcacttgtt tgatatgttt attccaactt tcttcaactt ttttcatatc ggctgcaata
     2941 ccgtgtttta acaactcttt atcaacttgg tctatctcaa aaagatcacc ggtataattc
     3001 caaagttcat taaccgcatt ctgcactctt tgatgactct cttcggtgcc atctcccaaa
     3061 cgcaatgtcc aatccgaagc aaaacgcaaa tggtaattaa cttctttcaa tgatttaatt
     3121 gcaagtgcac tgatgttttt atcagtagaa tgtgtcaatt cagaatataa atattgttgc
     3181 catgtactta caaaaagttg tctgattatt gtaaatccaa aatcaacatt aggttgttct
     3241 gtaagcaata aatttctgaa ttg
//
LOCUS       DAOOOL010000218         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3490_length_3263_cov_61.665835, whole genome shotgun sequence.
ACCESSION   DAOOOL010000218 DAOOOL010000000
VERSION     DAOOOL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_3263_cov_61.665835"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKU82_11760"
     CDS             complement(<1..493)
                     /locus_tag="PKU82_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOZ83581.1"
                     /translation="MKKLLNYFFQGLLVVVPIAVTILVIYKIITWIDNLLPFQIPVKL
                     PGIGEVSIPGLGILTIVVAVTAFGYLAKFFVANPFFVLLERMLERTPLLKIIYTSVKD
                     LIEAFVGEKKRFNQPVLVTVNKVSGVQRIGFITQKDLSVLGISSNKMAVYLPFSYGFN
                     GQLV"
     gene            complement(505..1257)
                     /locus_tag="PKU82_11765"
     CDS             complement(505..1257)
                     /locus_tag="PKU82_11765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOZ83582.1"
                     /translation="MIAHLKIGDEEYSAKLNEPIDISIPLHSGNDNVNAWYAPPPEIV
                     PVRMDSWVGEVAQGASVNFRNVFFNPHAHGTHTECVGHITKEWVSLNKVLKQFFFSAK
                     LITVLPMIVDGDMIITKEDVVQAWKNCGAEALVIRTLPNDNSKQSRHYSNTNPPYLHH
                     EAAAWMAEKNIKHLLLDLPSVDREQDGGKLLAHNAFWSTGKNIRYDCTISEMIFVPDH
                     IADGLYLLNIQITALENDASPSKPLLFQLTKK"
     gene            complement(1263..2390)
                     /gene="hemW"
                     /locus_tag="PKU82_11770"
     CDS             complement(1263..2390)
                     /gene="hemW"
                     /locus_tag="PKU82_11770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOZ83583.1"
                     /translation="MSGVYIHIPFCSKACTYCDFHFSTQLRNKNAFIEVLLHEIEVQA
                     RYIDEAVNTIYFGGGTPSLLSAAEINSIIGKLDKVFKLDVKEITLEANPDNLSEKYLK
                     ELKSTAVNRLSIGVQSFEEKRLLWMNRSHTAKQAIFAIESALQMGFNVSADLIFALPE
                     MSTDEWQRQLQKMISLKPHHISAYSLTLEERTVYHHQVAKKKIKELDDVNSEEQFLQA
                     HQLLSNAGYHHYEISNYASAGHVALHNSSYWNGKLYLGLGPSAHSFNGKMRQWNVSNN
                     TAYIKALSAGKLSFESEVLTEVDRYNEYVMTGLRTDKGIEEKQWNELMPSLYKESLQK
                     EMNELVNQQFLVRDASRIFIPLQHWYKADQLISRLFATENS"
     gene            2466..3245
                     /locus_tag="PKU82_11775"
     CDS             2466..3245
                     /locus_tag="PKU82_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83584.1"
                     /translation="MKSLRYIFLFIIFAPMFVKAGDKTIYSVDFKKAETFKVSGGVIN
                     ADYWEVRNDSAIFTSNNRLCNEKGNYRYQVEVTLAADGKLNADDQCIIYIMQNEAIAK
                     SFVVKGSDMTESKKFKYEVEGEKSPVLKVNIVLKSLGVDRAWRLTDNAVSIIAISKDD
                     DPELKVIQHANTSSVVWKCKTTDNHNYFIVERSANGTDFSEAGLVKANRNNQINYYSF
                     VDRTDAGTTNYYRIKLKSFNGDEMQYGEIVKAGKEDVANSR"
BASE COUNT         1009 a          681 c          591 g          982 t
ORIGIN      
        1 tcaccaactg cccgttaaaa ccataggaga aaggaagata aacagccatt ttattactgc
       61 tgatgccaag caccgataaa tctttttgtg tgatgaaacc aatgcgctgc actcccgaca
      121 ctttatttac cgttaccaac acaggctgat taaatcgttt tttctctcca acaaatgctt
      181 ctatcagatc ttttactgaa gtgtaaataa tttttaacaa cggtgttcgc tccaacatgc
      241 gttcgagcaa aacaaaaaaa ggattagcaa caaaaaattt agctaagtaa ccaaaagcag
      301 ttacagcaac tacaatggta agaatgccaa gtccgggaat gctcacttcg ccaatgccgg
      361 gcaacttaac cggaatctga aaaggcaaca ggttgtcaat ccaggttatg attttataga
      421 ttactaaaat ggtaacagca ataggtacaa caaccaacaa tccctgaaag aaataattaa
      481 gcagtttttt catctctaca cattttattt tttagtcaac tgaaacaata gtggtttgga
      541 agggcttgca tcattctcca atgcagtaat ctgaatattg agcaaatata gtccatcggc
      601 aatatggtcg ggcacaaaaa tcatttccga aatagtgcaa tcataacgaa tattttttcc
      661 tgtactccaa aatgcattgt gtgcaagcag ctttcctcca tcctgttcgc ggtcaacaga
      721 gggaaggtcg agtagcagat gtttgatatt cttttctgcc atccacgctg ctgcttcatg
      781 atgcagatag ggcggattgg tatttgaata atgtctgctt tgtttgctgt tgtcattagg
      841 taatgtacga atgactaatg cttcggcacc acagtttttc catgcctgaa ctacatcttc
      901 cttggtaata atcatatcgc catcaacaat cattggcaac accgttatca gctttgcaga
      961 aaagaaaaat tgtttcaata ctttatttaa cgaaacccac tccttggtga tatgtcctac
     1021 acactctgta tgtgtaccat gtgcatgagg gttaaaaaac acattcctga aatttactga
     1081 tgcaccttgc gccacttcgc ctacccaact atccatgcgc acaggaacaa tttccggtgg
     1141 aggtgcatac catgcattaa cattatcatt gccggagtgc aatggaatag aaatatctat
     1201 tggctcattg agcttagctg aatactcttc gtctcctatt tttaaatgtg caatcatttc
     1261 ttttaactat tttctgttgc aaacaacctg ctgattaact gatctgcttt ataccaatgc
     1321 tgcaagggta taaatatacg agaagcatca cgaactaaaa attgctgatt aaccaactca
     1381 ttcatttctt tctgcaaact ctctttatac aatgacggca ttaattcatt ccattgtttt
     1441 tcttcaatgc ctttatctgt tctcaggcct gtcatcacat attcattgta tcggtcaacc
     1501 tctgtcagca cttcactttc aaaagaaagt tttcctgcac tcaatgcttt aatatacgca
     1561 gtgttgttgc tgacattcca ttgccgcatt tttccgttaa aactatgagc cgatggtcct
     1621 agacctaaat aaagtttacc gttccaatag ctgctgttgt gcaaagcaac gtgtccggca
     1681 cttgcgtagt tcgatatttc atagtgatga tatcctgcat tgctcaacag ttgatgtgcc
     1741 tgcaaaaact gctcttccga attaacatca tctaactcct tgattttttt cttcgcaacc
     1801 tgatgatgat aaaccgttct ttcttcaagc gttaaactat aggcagaaat atgatgtggt
     1861 tttaaactta tcatcttctg caactgcctt tgccattcat ctgttgacat ttcaggtaag
     1921 gcaaaaatta aatcggcact aacattaaat cccatttgca gcgcagactc aatagcaaat
     1981 attgcctgtt ttgctgtatg acttctgttc atccacagca gcctcttttc ttcaaacgac
     2041 tgcacaccaa tgctcagtct gttcactgct gttgacttca attctttcag atatttttca
     2101 gaaagattat cggggttggc ttctaaagtt atttctttaa catcaagttt aaacacttta
     2161 tctagctttc cgataatact gttaatctct gctgctgaaa gtaatgatgg tgttcctcca
     2221 ccaaaataga ttgtattaac tgcctcatca atatagcgtg cctgcacttc tatctcatgc
     2281 aacaggactt caataaatgc gtttttgttt cgtagctgtg tcgaaaaatg aaagtcgcaa
     2341 taggtgcaag ccttgctgca aaaaggaata tggatataaa cacctgacat ttgaaaagta
     2401 aggcaaaagt agcataaaga gactgctcat ttcttcaata tttgtatgat taaataatca
     2461 accggatgaa aagtctcaga tacatttttc tgtttatcat attcgctccc atgtttgtaa
     2521 aggcaggcga caaaacaatc tattcagtag attttaaaaa agcagaaacc tttaaagttt
     2581 ccggtggtgt catcaatgcc gattattggg aagtgcgcaa cgactctgcc atatttacat
     2641 caaacaacag actgtgtaat gaaaagggca actatcgtta tcaggtagaa gtaaccctag
     2701 cggcagatgg aaaactcaat gccgatgacc aatgtattat ttacattatg caaaacgagg
     2761 ccatagctaa atcgtttgtg gttaaaggca gcgatatgac agaaagcaaa aaatttaaat
     2821 acgaagttga aggagaaaaa tcgcctgttc tgaaagtaaa tattgtactc aaatcgttgg
     2881 gtgttgaccg cgcctggcgg cttacagaca atgccgtttc tatcattgcc atctctaaag
     2941 atgatgaccc tgaattgaaa gttattcaac atgcaaacac atcgagtgtt gtgtggaagt
     3001 gcaagactac agacaaccat aactatttta ttgtagagcg ctctgccaac ggaaccgact
     3061 ttagcgaagc cggattggta aaagccaacc gcaacaatca gataaactac tactcctttg
     3121 ttgacagaac cgatgcaggc accacaaact actaccgcat aaaactaaaa tcttttaacg
     3181 gtgacgaaat gcaatatggc gaaatagtaa aagccggaaa agaagatgtt gcgaatagta
     3241 ggtagggggg ggtaggtttt tgc
//
LOCUS       DAOOOL010000219         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3556_length_3233_cov_49.203587, whole genome shotgun sequence.
ACCESSION   DAOOOL010000219 DAOOOL010000000
VERSION     DAOOOL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3556_length_3233_cov_49.203587"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKU82_11780"
     CDS             <1..412
                     /locus_tag="PKU82_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83585.1"
                     /translation="NIELATLKTNFDSLTTTNQNQATVIADQTTKIHTAYYTVGNYKS
                     LRDKQVLSKKGGVLGLGKKATVVPAIKNDNFTKIDYTQISTIPVNSKKVELVTTHPDG
                     SYKIEKNNDLVSDILITDPEKFWSASKYLVVLTK"
     gene            574..1608
                     /locus_tag="PKU82_11785"
     CDS             574..1608
                     /locus_tag="PKU82_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOZ83586.1"
                     /translation="MQKKFFFLILFAFGFIKLYAQTEPAKTTEKESLVNWISLSEAEK
                     HNKEVPKPIIIDFYTSWCGWCKVMMNTTYSDPAIASYINANFYPVKFDAETKDTIEYD
                     GVKYKPTGTEKRDPNEFAVKMLNGTMMYPSTIFMNKAANFTMSAQGYLETKKIEPMLV
                     FTLENGFRNSSYEDFNAQFQKAFYDSLQTKSYESVKWQTPLQFFIKDKKPDQKKKIIF
                     INTEWCNTCRVMYRTTFSDTAVSSLLSKHFELVNFNPETNDKLYFQDKEFDNIHSKEL
                     PFHQLVYALSRNGLLFPQVIFMDEKNTVVDAIPFYLNPNVFKNIVRFYGEDIYKTKNW
                     ETFIKEQETK"
     gene            1666..2889
                     /locus_tag="PKU82_11790"
     CDS             1666..2889
                     /locus_tag="PKU82_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HOZ83587.1"
                     /translation="MKRLKNPVTFIFITVLVDCIGIGIIFPVIASLITEVSHVPVNKA
                     TTYSGWMMTLYALMQFIFSPVLGGISDRYGRRPVLLLSLLGLGIDYLFLSVAHTLTLL
                     FIGRIIAGICGASFTTSFAYIADVSPSEKRAQYFGMMGAAFGVGFIIGPVLGALASHW
                     GVRAPFVVAACLSLLNFLYGYFILPESLKPENRRAFHWKRANPLGAFIQIKKSKNIRT
                     LIFTMFLLYLAGQVMPSIWAFYTKFTYQWSDAEIGYSLAFVGVMVAIVQGGLIKWSQT
                     KLGSHTSIYTGLAFYLVGLTLFAFANQSWMLYAFTFIYALGGIGPPSIQGIISGLVPP
                     NEQGELQGMMTALMSVSTILSPLIMTNLFYNFTKPDTIYYFPGAPFAAAAILVLISFF
                     VVKRGINNLTRHQKI"
     gene            complement(2987..>3233)
                     /locus_tag="PKU82_11795"
     CDS             complement(2987..>3233)
                     /locus_tag="PKU82_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOZ83588.1"
                     /translation="IRVIKTLRKWGVYVDEAYFLGGLSKDNVLKAFGAHIFFDDQEVH
                     LSESSKVVPSGKVPYTSDSPLLKLDVHPKANKAASKK"
BASE COUNT         1045 a          566 c          582 g         1040 t
ORIGIN      
        1 gaatattgag ttggctactt taaaaacaaa cttcgattct cttacaacaa caaatcaaaa
       61 tcaggctact gtaattgctg atcaaacaac taaaatccac actgcatatt acactgtagg
      121 gaactacaaa agccttcgcg acaaacaagt gctttctaaa aaaggtggtg tattgggctt
      181 aggtaaaaaa gcaactgtag tgcctgcaat taaaaacgat aatttcacca aaatcgatta
      241 tacccaaatc agcactattc ctgtgaatag taaaaaagtt gagttggtaa ctacacaccc
      301 tgacggatca tacaaaattg aaaaaaataa tgatcttgtt agcgacattt tgattacaga
      361 tcctgagaaa ttctggagtg catcaaaata tcttgtagta ctgaccaaat aataaaaaca
      421 gaaaataaaa tattctttgg ttttaggttg ggtagcgggg cagcaatgct ccgctttttc
      481 tttttaatag atgaatgttt aataaaattc tttacccctg tatgcaatgt tgcaaagtca
      541 ttatacattt gcccatcctg acattaagaa gttatgcaaa agaaattttt cttcctgata
      601 ttatttgcat ttggtttcat aaaactctat gcacaaacag agcctgctaa aaccacagaa
      661 aaagaatcgt tagttaactg gattagttta agcgaagctg aaaaacataa taaggaagtt
      721 ccaaagccta taattattga tttttatacc agttggtgtg gctggtgtaa ggtgatgatg
      781 aatacaacat attctgaccc tgcaatagca tcgtacatca acgccaattt ctatcccgtt
      841 aaatttgatg cggaaacaaa agatacaatt gagtatgatg gtgtaaaata caagcccaca
      901 ggaactgaaa aacgcgatcc caatgagttt gcagtaaaaa tgctgaatgg cacaatgatg
      961 tatccttcga ctatttttat gaataaagct gcaaacttta ccatgtcggc acagggatat
     1021 ctggaaacca agaagattga acctatgttg gtgtttacat tagaaaatgg ctttcgcaac
     1081 agtagctatg aagattttaa cgcacaattt caaaaagctt tttatgattc attgcaaacc
     1141 aagtcttacg aatctgttaa atggcaaaca ccattacagt tttttataaa agacaaaaaa
     1201 cctgatcaga aaaagaaaat tatatttata aatacagaat ggtgtaatac ctgccgtgtg
     1261 atgtatagaa ctacatttag cgatactgct gtgagtagtt tattatcaaa acattttgaa
     1321 ttggtaaact tcaatcctga aactaacgac aaactttatt ttcaggacaa agaatttgat
     1381 aacatacatt caaaagaatt gccttttcat cagttggtgt atgcactaag tcgtaacgga
     1441 ttattatttc cgcaagtgat atttatggat gaaaagaata ctgttgttga cgctattccg
     1501 ttttatctga atccaaatgt ttttaaaaat attgttcgtt tttacggaga agatatttat
     1561 aaaacaaaaa actgggagac ttttattaaa gagcaggaaa caaaataatt cctcgtaatt
     1621 atcctacaca atatttaaaa acctaataac acaaatcgtc aacgtatgaa acgtttaaaa
     1681 aatcctgtta catttatttt cattaccgtt ttagttgatt gcatcggtat tggaattatt
     1741 tttcccgtaa ttgcatcact gataacagag gttagtcatg ttccagtaaa taaagcaaca
     1801 acctacagcg gctggatgat gaccctttat gcattgatgc agtttatttt ttctcctgtg
     1861 cttggtggca tcagtgatcg ctatggtcgc aggccggtgc tgcttttgtc gttgttaggt
     1921 ttaggtattg attacttatt tttatctgta gcacatacac taacactatt atttatcggt
     1981 agaattattg caggaatatg tggtgcaagt tttacaacaa gttttgctta cattgccgat
     2041 gtcagccctt ctgaaaaaag agcgcagtat tttggtatga tgggtgcagc attcggtgta
     2101 ggttttatta ttggccctgt tcttggtgca ttggcaagtc actggggcgt gcgtgcacca
     2161 tttgttgtag ctgcttgttt atcattgctg aattttctat atggatattt cattttgcct
     2221 gaatctttaa aacccgaaaa caggcgagcc tttcattgga aacgtgccaa cccacttggt
     2281 gctttcattc agattaagaa aagtaagaat atcagaacgc tcatctttac catgtttctg
     2341 ctttatcttg ccggacaagt aatgccatct atctgggcgt tctacaccaa gtttacctat
     2401 caatggagtg atgcagagat tggatactca ttagcttttg ttggtgtgat ggttgctatc
     2461 gtacaaggtg gactaattaa atggtcgcag acaaaattgg gcagtcacac ttctatttat
     2521 acaggacttg ctttttatct ggtaggatta accttgtttg cctttgccaa ccaatcgtgg
     2581 atgctttatg catttacttt tatttatgca ttaggaggca ttggtccgcc atcaatacag
     2641 ggaatcatat ctggcttagt accacctaat gagcaaggcg aacttcaggg aatgatgact
     2701 gcactgatga gtgtgtcaac aattttatcg ccattgataa tgaccaattt gttttacaat
     2761 ttcaccaaac cggatacgat atattatttt cccggagcac catttgcagc agcagcaatt
     2821 ttagtgttga tttcgttttt tgttgtgaag aggggaataa ataatctgac ccgccatcag
     2881 aaaatataaa tataatattt aatgatacag atttttaatc tgtaaaatat tgcaacacga
     2941 attgttcaat aaatcttctc ttcactataa aacgacttat atcattttac tttttggagg
     3001 ctgctttatt ggctttagga tgcacgtcta atttcaacag aggcgagtct gatgtgtaag
     3061 gtactttccc cgaaggaacc acttttgacg attccgataa atgcacttcc tgatcatcaa
     3121 aaaatatatg tgcaccaaat gcttttaaaa cattatcttt tgaaagccct cctaaaaaat
     3181 atgcctcatc aacatataca ccccacttac gaagtgtttt aatgacacga atg
//
LOCUS       DAOOOL010000220         3226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3567_length_3226_cov_60.250710, whole genome shotgun sequence.
ACCESSION   DAOOOL010000220 DAOOOL010000000
VERSION     DAOOOL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3567_length_3226_cov_60.250710"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /gene="bamA"
                     /locus_tag="PKU82_11800"
     CDS             complement(<1..2032)
                     /gene="bamA"
                     /locus_tag="PKU82_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOZ83589.1"
                     /translation="MHKHILGFLTFFILLSTFSIAQVTVGDDQATLDYNNPKEYEIAA
                     ITVSGIKYLDQNALKILSGLSVGEKVKVPGEKFTKAIENLWKQGLLTDVKIVATRIEG
                     KKIFIDLQLQEKPRLSKFSFTGVRKGHADKIREKIQISKGRVLSDNALNTVNTQVLNF
                     YKEKGFLNAKVNLTTIKDTTETNSEILVINVDKGKRVKIHRVNIIGNEEVSSVKLKRA
                     MKGTREQRWRYFFNSKKFDEDKYTDDKSNLISKYNEKGYRDAQILSDSVYPVSRRRIN
                     IDIKVSEGNRYYFRNISWIGNTKYTSAELNKVLGIKPGDVYNAKRLESSLYMSESSRD
                     ITSLYMDDGYLFFNIDPVEVSAENDSIDLEMRIYEGKQARINHVTVKGNTKTNDRVIL
                     REIRTKPGQLFSRSDIIRTQRELSQLGYFNPEKMGVNPKPNPVDGTVDIEYEVEERPN
                     DQVELSGGWGANQVVGTLGVTFNNFSARNILNTKAWRPLPAGDGQRLSIRATTNGIYY
                     QSYNASFTEPWLGGKKPNALTVSIYHSIQTNGLKKSNDNRQAIFISGATVGLQKRLKW
                     PDDYFTLYSGINYGLYTLKNYRSTFLFTDGTSNNISIEENLTRSSVDQPIYPRKGSEI
                     SFSVQFTPPYSAFTNKDYSKLDTKEKYKWIEYHKWKFSIGWYTKLLGNLVMYN"
     gene            complement(2025..2777)
                     /locus_tag="PKU82_11805"
     CDS             complement(2025..2777)
                     /locus_tag="PKU82_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOZ83590.1"
                     /translation="MSIKNQIQTDRLPRHIAIIMDGNGRWAKKQGKLRMFGHQHGVAA
                     VRDTVEGAAELGIEYLTLYAFSTENWNRPAIEINALMELLVNTISKETKTLMDNNIRL
                     NAIGDIESLPGRCNKNLKTAIDKTAGNSRMTLTLALSYSSRWEITRAIKRIAQDVKDG
                     KLNATEINDSLVEKYLTTAGIPDPELLIRTSGEYRVSNFLLWQIAYSEFYFTNKLWPD
                     FRREDLFESIVSYQNRDRRFGGVNESTNAKHA"
     gene            3058..>3226
                     /locus_tag="PKU82_11810"
     CDS             3058..>3226
                     /locus_tag="PKU82_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOZ83591.1"
                     /translation="MDFRINSPFKPTGDQPQAISELVKGLDRGDRHQTLLGVTGSGKT
                     FTVANVIEKVQR"
BASE COUNT          862 a          633 c          601 g         1130 t
ORIGIN      
        1 tattatacat tacaagatta ccaagaagtt tggtgtacca tccaatgctg aatttccatt
       61 tgtgatattc tatccacttg tatttttctt ttgtgtctaa ttttgaataa tccttatttg
      121 taaatgcaga gtagggtgga gtaaactgaa cagaaaaaga aatctctgat cctttgcgcg
      181 gatagattgg ttgatctaca ctgctgcgtg taagattttc ttctatagaa atgttgttcg
      241 aagttccatc ggtaaataaa aatgtggagc gataattttt aagtgtataa agcccataat
      301 ttattcctga gtagagtgta aaataatcgt caggccactt aagtcgtttt tgtaaaccaa
      361 cagttgcacc agaaataaaa attgcctgtc tgttgtcatt agattttttt aaaccattgg
      421 tttgaatgga gtgatatatt gaaactgtga gtgcatttgg ttttttgcca ccaagccagg
      481 gttctgtaaa cgaagcatta taggactgat agtaaatacc gtttgtagta gcacgaatgc
      541 ttaatctttg cccatcaccg gctggcagcg gccgccatgc cttggtattt aaaatatttc
      601 ttgcagaaaa attgttgaac gtaacaccta aagtacccac aacttgatta gctccccatc
      661 ctccggaaag ttctacctga tcgtttggtc tttcttctac ttcatattct atgtcaacag
      721 tgccatcaac aggattgggc tttgggttta ctcccatttt ttcgggattg aaatagccta
      781 actgcgacaa ttcgcgctgc gttctgatga tgtcacttct gctgaaaagt tgcccgggct
      841 ttgtacgtat ctctcgtaaa attactctgt cgttggtttt tgtatttcct ttaacggtaa
      901 catgatttat ccttgcctgc ttaccttcgt aaatacgcat ttccaaatct attgaatcgt
      961 tttctgcaga tacttcaaca ggatcaatat tgaaaaacag gtatccatcg tccatataaa
     1021 gcgaagtaat gtcgcggctt gactcactca tgtatagtga actttcaagt cgttttgcat
     1081 tatatacatc acccggtttt atgcctaaaa ctttattcag ttctgcagag gtgtattttg
     1141 tgtttccaat ccaactgata tttctgaaat aataacgatt gccttcacta acttttatat
     1201 caatgttgat tctcctgcgg ctgacaggat aaacactgtc acttaaaatc tgtgcatcgc
     1261 gatatccttt ctcattgtat tttgaaatca gattgctttt gtcatcagta tatttatcct
     1321 catcaaattt tttggagttg aaaaaataac gccagcgctg ctcgcgtgtg cctttcatgg
     1381 ctcgttttaa tttaacgctg cttacttctt cattgcctat gatgtttact ctgtgaattt
     1441 ttacacgctt gcctttgtca acattgataa ctaaaatttc gctgttggtt tctgtggtgt
     1501 cttttattgt agtaaggttg actttagcat ttaaaaatcc tttttcctta taaaaattca
     1561 gcacttgtgt gtttacggtg tttagtgcat tatcagaaag tactctgcct tttgatatct
     1621 gaattttttc gcgaatctta tcagcatgac cttttcgcac tcctgtgaat gagaatttac
     1681 tgagtcgtgg cttttcctga agctgaaggt caataaatat tttttttcct tcaattcttg
     1741 tagctacaat ttttacatca gtaagtaaac cttgtttcca aaggttttct attgccttgg
     1801 taaatttttc gcccggcact tttacttttt ctccaacact tagcccggat aaaattttca
     1861 atgcattctg atcgagatat tttatcccgc tgacagtaat agcagctatt tcgtactctt
     1921 taggattgtt ataatctaat gttgcctgat cgtcaccaac agttacctgt gcaatggaaa
     1981 aagttgaaag cagtataaaa aaggttagaa aacctaaaat gtgtttatgc atgtttggca
     2041 tttgttgatt cgtttacgcc accaaacctt ctgtcgcggt tttgatagct aacgattgat
     2101 tcaaaaagat cttcgcgcct gaagtcaggc caaagtttgt tggtgaaata aaattcgctg
     2161 taggcaatct gccacaataa aaaattagaa actctgtatt cgccactcgt tctgatgagc
     2221 agttccggat cgggaattcc tgcagtggtt aaatattttt ctactaatga gtcgttgatt
     2281 tctgttgcat taagcttgcc gtctttaaca tcctgagcaa tgcgttttat ggcgcgtgta
     2341 atttcccatc gggagctgta acttaatgct aatgtaagtg tcattctgct gttgccggca
     2401 gttttgtcaa tagcagtttt aagatttttg ttgcagcgtc cgggcaggct ttcaatatct
     2461 ccaatggcat tcagtctgat gttattatcc atcagcgttt tggtttcttt tgaaatggtg
     2521 tttaccaaca attccatcag tgcattaatt tcaatggcag gcctgttcca gttttcagtg
     2581 ctgaaagcat aaagtgtcag gtattcaata cccagttctg cagcaccttc cacagtgtcg
     2641 cgaacggcag ccacaccatg ctgatgccca aacattctga gcttgccctg cttttttgcc
     2701 caacgcccgt taccatccat aatgatggct atgtgtcggg gcaacctgtc tgtttgaatc
     2761 tgattcttaa tactcatccg ttaaaacgaa ggcgcaaatt tagaatttta accgaatgtt
     2821 gaagtttaag tttttaacgc attttgaacg attttatgat gtttcacaaa acattaatac
     2881 tcaccttagt cttttaacat ttttttacat ttggagaaag tgcgcattta gccaattttc
     2941 tcatcaagaa tgaagagcgc catgctattt atgataacat gttaaatttt ttttcttcac
     3001 ttaaaattgt actcagtgtt cgctgcctac aagccttaaa ttccgcatct ttgtagaatg
     3061 gatttccgca tcaactcacc ttttaagcca accggtgatc agcctcaggc cataagcgag
     3121 ttggtaaaag ggttggacag aggcgacagg catcaaacac ttttaggggt aaccggcagt
     3181 ggaaaaacat ttactgtagc caatgtaatt gagaaagtac agcgac
//
LOCUS       DAOOOL010000221         3218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3585_length_3218_cov_63.953209, whole genome shotgun sequence.
ACCESSION   DAOOOL010000221 DAOOOL010000000
VERSION     DAOOOL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_3218_cov_63.953209"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKU82_11815"
     CDS             <1..285
                     /locus_tag="PKU82_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83592.1"
                     /translation="RISDSELEKLFEEKSFEDALDYCTSLCDIEVQKKYTGNHINWFT
                     EEKLIRILKAAGFNNIYRSGYGQSYSPVMRDLNFFDETLPGISLYVEAQK"
     gene            complement(288..3170)
                     /gene="uvrA"
                     /locus_tag="PKU82_11820"
     CDS             complement(288..3170)
                     /gene="uvrA"
                     /locus_tag="PKU82_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOZ83593.1"
                     /translation="MKKYIIIKGASVHNLKKVDVAISRNSFTVITGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSSYARQFMGRLDKPEVEYIKGIAPAIAIEQKVNTRNPRSTVGTS
                     TEIYDYLKLLFARIGKTYSPVSGKLVKKNTVADVVDILKTQNQNKRFIIAAPLQFHPG
                     RTLHEELNALMQKGFSRIVIDDKILRIDEYAEQIEKTDAQPAVKTRGRKKKVESIEPT
                     INSNIFIVVSRIIIPENINEEFESSTADSVQTAFNEGSGRCTLIDITDETLHFTNFSN
                     YFEEDNIRFEEPSTHLFSFNNPYGACKNCEGFGSVIGIDENLVIPNKTLSIFDGAVAC
                     WRGEKMNEWQQEFILKAYKFNFPVHRPYYELNETDIKLLWEGNKTIKGLNDFFKYVES
                     QSFKIQYRVLLSRYRGKTTCPECKGTRLRADANYVKVNSKSITDIVLMPVNVSVEFFN
                     NIVLDSYEQKVAGRLLVEIKNRLQYLNDVGLGYLTLNRLSKTLSGGESQRINLATSLG
                     SSLVGSMYILDEPSIGLHPRDTQRLIYVLKSLQKAGNTLIVVEHDEEIMRNADELIDI
                     GPFAGTHGGKIVFQGNFEQLEHSTESVTAKYLTGKEFIHVPSKRRNWTNSIEITGASE
                     NNLAHIDAKFPLGILTAVTGVSGSGKSTLVKKVLYPALKKVLGGYDDESGKFEKLTGD
                     FTTLNGVEIIDQDPIGKSSRSNPVTYVKAYDEIRALMADTPFARQKMMKPSLFSFNVD
                     GGRCEMCQGEGVVTIEMQFMADIQLKCETCQGKRFKNEILAITYREKNIFDILSMTVD
                     DAISFFSKETDERYILFTNKIIARLQPLSDVGLGYISLGQSSSTLSGGEAQRIKLASY
                     LGIGHATTKTLFIFDEPTTGLHFHDIRKLLDAFTALIRNGNSILVIEHNMDVIKCADW
                     VIDMGPGGGNEGGKIVFAGTPEALSNCNESYTGKFLSEKLIAQQEL"
BASE COUNT          954 a          620 c          514 g         1130 t
ORIGIN      
        1 cgtatctccg atagtgaatt agaaaaattg tttgaagaga aatcctttga agatgctctg
       61 gattattgta cttcattatg cgatattgaa gtgcaaaaga aatatacggg taatcacatt
      121 aactggttta cagaggaaaa gcttattaga atattaaagg cagccggatt taataacata
      181 tatcgttccg gttatggaca aagttactca cctgttatgc gtgatcttaa ttttttcgat
      241 gagacattac ccggtatttc actctatgta gaagcacaga agtaaagtta tagctcttgt
      301 tgggcaatta atttttctga gagaaatttt cctgtataag attcgttgca attactcaaa
      361 gcttcaggag ttcctgcaaa aactatttta ccaccttcat ttccaccacc cggccccata
      421 tcaataaccc aatcagcaca tttaataaca tccatattat gttctataac caaaatagaa
      481 ttgccattgc gaattaatgc agtaaatgca tcaagtagtt ttctgatatc gtgaaagtgc
      541 aaacctgtag tcggttcatc gaaaataaaa agtgtttttg ttgttgcatg accaatgcca
      601 agatacgatg ctaatttaat tctttgcgct tctccgccac tgagagtact agaggattgt
      661 cctaaagaaa tataacctaa gccaacatct gaaagtggtt gtagtctggc aattatttta
      721 ttagtaaaaa gaatataacg ttcatcagtt tctttagaaa aaaagctaat ggcatcatca
      781 accgtcattg ataggatgtc gaaaatattt ttttcccgat aggtgattgc caatatttca
      841 ttcttaaagc gtttaccttg gcaagtttcg cattttagct gtatgtctgc cataaactgc
      901 atctcaatgg ttactacacc ttcaccctga cacatctcac atcttccacc atcaacatta
      961 aaagaaaata aagatggttt catcattttt tgtcttgcaa aaggagtgtc ggccatcaat
     1021 gctctgattt catcataagc ctttacatag gtaacaggat tactccttga tgatttacct
     1081 atcgggtcct gatcaataat ttcaacacca ttcagtgttg taaagtcgcc tgttagtttt
     1141 tcaaatttcc ctgattcatc atcatagcca cctaaaactt ttttaagtgc cggataaaga
     1201 acttttttta ccaaggttga tttacctgac ccactgacac cagtaacagc agtcagaata
     1261 ccaagtggaa atttagcgtc aatgtgtgct aaattatttt cacttgcacc ggttatttca
     1321 atgctgttag tccagtttcg tcttttactt ggcacatgaa taaattcttt gccggtaaga
     1381 tattttgctg ttacactttc tgtgctatgt tctaattgct caaaatttcc ttggaaaaca
     1441 attttgccgc catgtgtacc tgcaaagggg cctatgtcaa taagttcatc agcattgcgc
     1501 ataatctctt catcatgttc aacaacaatc agcgtattac ctgccttttg aagtgatttt
     1561 aaaacataaa tcaaacgctg tgtatctcga ggatgcaacc ctatacttgg ctcatccaga
     1621 atatacatgc ttccaaccaa actgctgcct aatgatgttg ccagatttat gcgttgcgat
     1681 tcgcctccgg aaagtgtttt tgaaagtctg ttcaatgtca gatatcctaa tccaacatca
     1741 tttaaatatt gcaatctgtt ttttatttca actaataacc gcccagcaac tttttgttca
     1801 tagctgtcta aaacaatgtt attaaaaaat tcaacactga catttaccgg cattaaaaca
     1861 atatcggtaa tagatttact attcaccttt acataattgg catcggcacg taaacgtgtt
     1921 cccttacatt ctgggcaagt tgtttttcca cgatagcggc ttagtaaaac tctgtattga
     1981 attttgaagg attgcgattc tacatattta aaaaagtcat ttaatccttt aattgtttta
     2041 ttaccttccc ataataactt gatgtccgtt tcatttaatt cgtaatatgg acgatgtaca
     2101 gggaaattaa atttgtatgc ttttaaaata aattcctgtt gccattcatt cattttttcg
     2161 cctcgccaac aagctacagc accatcaaaa attgaaagtg ttttgtttgg gataactaag
     2221 ttttcatcaa tgccgattac acttccaaag ccctcgcaat tcttgcatgc tccataagga
     2281 ttattaaagc taaaaaggtg tgttgatggt tcttcaaaac ggatattatc ttcttcgaag
     2341 taattactga agttggtaaa gtgcagcgtt tcgtctgtta tatcaattaa agtgcaacgc
     2401 ccgctacctt cattaaatgc agtctgaaca gaatctgctg tagaagattc aaattcttca
     2461 ttgatatttt cagggataat tattcttgaa acaactataa agatgtttga attgattgtt
     2521 ggttcaatag attcaacttt cttttttctt cctcttgttt taacagcagg ctgtgcatct
     2581 gttttttcta tttgttctgc atactcatct atcctcaaaa ttttatcatc aataacaatt
     2641 cggctaaaac ctttttgcat taatgcattt aattcttcat gtaaagttct tccgggatga
     2701 aattgcaaag gcgctgcaat gataaatctc ttgttttgat tttgtgtttt caagatatct
     2761 acaacatcag caacagtatt cttttttacc aatttaccac ttacaggtga gtatgttttt
     2821 cctattcgtg caaatagtaa tttaagataa tcgtaaattt ctgttgaagt gccaacagtt
     2881 gatcttggat ttctggtatt tactttttgt tcaattgcaa tcgctggggc aatgccttta
     2941 atgtattcta cctcaggttt atctaaacgt cccataaatt gccttgcata acttgacagg
     3001 ctttctacat aacgtctttg cccttctgca tacaatgtat cgaaagctaa ggacgattta
     3061 cctgaacctg ataatccggt aatcactgta aaactatttc tgcttatggc tacatctact
     3121 tttttcaggt tgtgaaccga tgcacctttt atgattatat attttttgat ttacgctgat
     3181 tgagattgcc gcttaaaaac acatttactt ttccttcg
//
LOCUS       DAOOOL010000222         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3633_length_3193_cov_117.160612, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000222 DAOOOL010000000
VERSION     DAOOOL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_3193_cov_117.160612"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKU82_11825"
     CDS             complement(<1..282)
                     /locus_tag="PKU82_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOZ83594.1"
                     /translation="MKRFRSLFGLKIMVVMVLLFSSFKAPDFTLKLAKLKYAGGGNWY
                     VSPTALHNLAVFCNQNINTNISPEEAVVEPGSPDLFNYPFVHMTGNGRFV"
     gene            381..>3193
                     /locus_tag="PKU82_11830"
     CDS             381..>3193
                     /locus_tag="PKU82_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOZ83595.1"
                     /translation="MTLRILLVAIFAPLMLNAAHDHNGFDRNEDPNLKVFVKDRKRLP
                     DVNRQESLAQQKSWQQFSEQNKGWSVVFNEETGLPHNAFGKPISVGLPLDAQSTAWSF
                     ISTKLNSFGLPLNDLYFRNTAQNKKYFYVNYIQRYNGLEVLWSKVQIKITKDGKVNQF
                     ICDAYKDITVNINPTLSTQAASAAAINGLNLTLNGTTVNSDLKVLPVPDAAKNIYHLV
                     YEVTVKATDAENFPREYYTLVDAHNGEVLYRHNKINYFAANTDVNVTATVYPTQPYLP
                     TAVEPLANMNVTISGVPFLTDANGYLGLPNTSPVTATFKLEGSWANVKTGATTPQFTA
                     TLNPGANNITFSSANIKELSAYNSVNEIHDYYVTKMMGSGAETVMDIQMQTIVDVSGN
                     CNAFYNGDLNFYAAGGQCNATSIINDVVYHEYGHGINYDLYSYYGGIFSNGALGEGYA
                     DTWANGLTEDPVLGIGFYSGNATGFVRRYDQGRKVYPDDIMGEVHADGEIIAGAWWDV
                     GLNFNSVQQRMDLYVQTFAATIDYPDGQEGQLYTDILVEALTIDDNDGDLSNGTPNYC
                     AISSAFGIHGINMFVVGKLITHNDVLSAPGQQNITLNASGPASFGNGTGNSVNGYYSL
                     NHSATWNPISFTYNGTIFQGSIPGQPNGTIVSYYLDATDQCGTHFSLTPPNVTDSNPN
                     LPYYVLVGYNLVASDYFDTGNSQGWTLGMPGDNATTGQWIIASPVGSILNPGGSPPVY
                     VQPPIDHTNTSNNLCAVTGNAAPNDGAGTNDIDDGKTTLLSPNFDLSAYSNPAISYWR
                     WYTNDQGATPGTDYWQVSVSNDGGTTFVPIENCIVSDHSWRRFAFRVADYVQPTANMR
                     LKFIGEDANDGSLVEGLLDDLEIWSAEPVGISETPMLSWYAYPNPANEQLNFGWKGNG
                     TTVDVKLFNTVGQIVYQNQFSGNT"
BASE COUNT         1016 a          611 c          624 g          942 t
ORIGIN      
        1 cacaaaacga ccatttcctg tcatgtgcac aaatggataa ttaaacaagt cagggctacc
       61 cggttctact actgcctctt caggacttat gttggtatta atattctgat tacagaaaac
      121 agccagatta tgtaatgccg tagggctgac ataccaatta ccaccacctg catatttgag
      181 cttggcaagt ttcagtgtaa aatcaggtgc tttaaatgaa gaaaacaaca gtaccatcac
      241 taccatgatt ttcaacccaa aaagactacg gaaacgcttc atttgacccc aaaaatatgt
      301 ttaatcagtg acacaatctt atttgcacct taattttata tgaataattt tgtccttcca
      361 aaaaatcaaa tctatttaat atgacattgc gaattttact cgttgcaatt tttgcaccgc
      421 tgatgcttaa tgcagcacat gatcacaacg gtttcgaccg taatgaagac cccaatctta
      481 aagtatttgt taaagacaga aaacgtttac ctgatgtaaa ccgtcaggaa agtcttgcgc
      541 aacaaaaatc gtggcaacag ttttcggaac aaaacaaagg atggtctgta gtttttaacg
      601 aagaaaccgg attaccacac aatgcttttg ggaaaccaat ttctgtggga ctgcctttag
      661 atgctcaatc tactgcttgg tcgtttatca gtacaaagct taattctttt ggtcttccgt
      721 taaacgattt atatttcagg aatacagcac aaaacaaaaa gtacttttat gtaaactaca
      781 ttcagcgtta taatggactt gaagtattat ggtcgaaagt gcagataaaa attaccaagg
      841 atggaaaagt taatcagttt atttgcgatg catataaaga cattactgta aacattaatc
      901 caacattgag tacacaagct gcttctgctg ctgcaattaa tggtctaaat ttaacattaa
      961 atggcaccac agtaaacagt gatttaaaag ttttgcctgt gcctgatgca gcaaaaaata
     1021 tttatcattt ggtttatgag gtaactgtca aagcaacaga tgcagaaaat tttccaagag
     1081 aatattatac gcttgttgat gcacataatg gcgaagtgct ttatcgtcat aataaaatca
     1141 attattttgc tgccaacaca gatgtcaatg taacagcaac agtttaccca acacagcctt
     1201 atttacctac tgctgttgag ccattagcca acatgaatgt taccatttcc ggtgtacctt
     1261 ttctgactga tgcaaatggt tatttaggac ttcctaacac ttcacctgta acggcaactt
     1321 ttaaattaga aggtagttgg gcaaatgtaa aaaccggtgc tacaacacca cagtttacag
     1381 caactttaaa tccgggtgct aacaacatca cattcagtag tgcaaatatc aaagaacttt
     1441 ctgcatacaa ttctgtgaat gaaattcatg attattatgt aaccaagatg atgggttcag
     1501 gcgctgaaac agtaatggat attcaaatgc aaaccatagt agatgtttca ggaaactgta
     1561 atgcatttta taatggcgac ttaaactttt atgctgcagg tggccaatgt aatgcaactt
     1621 ctattattaa cgatgtggtt tatcacgaat atggtcatgg aataaactat gacttgtatt
     1681 cctactatgg aggtatattc agcaatggtg cacttggaga gggatatgca gatacatggg
     1741 ctaacggatt aacagaagat cctgttttgg gaataggttt ttattccggt aatgcaacag
     1801 gttttgtaag acgttatgat cagggaagaa aagtttatcc agatgatatt atgggtgaag
     1861 tgcatgccga tggtgaaatt attgcaggtg cctggtggga tgttggttta aactttaaca
     1921 gtgtacaaca gcgtatggat ttatatgtac agacttttgc agcaactatt gattatcctg
     1981 atggacaaga aggacaactc tacaccgata ttttagttga agcattaact attgatgata
     2041 atgatggaga cctgagcaat ggaacaccaa attattgcgc catttcttct gcctttggca
     2101 tacatggaat aaacatgttc gtagtaggaa aattaataac ccataatgat gtactcagcg
     2161 ctcccggtca acaaaacatt acactcaacg cttccggtcc tgcaagtttt ggaaatggca
     2221 caggtaacag tgtaaacgga tactacagtc tgaaccattc tgctacatgg aatccaatca
     2281 gctttactta taacggaacc atttttcagg gaagtattcc cggtcaacct aacggtacaa
     2341 ttgtttcata ttatcttgat gcaacagatc aatgtggaac acactttagt ttaacaccac
     2401 ctaatgtaac agattccaat cctaatcttc cttactatgt tttggttgga tataatttag
     2461 tggcaagtga ttattttgat acaggcaaca gtcaggggtg gactttaggt atgccgggtg
     2521 ataatgcaac aacaggacaa tggataattg catcacctgt aggttctatt cttaatcccg
     2581 gaggatcacc accggtatat gttcaacctc ctattgatca cacaaacact tctaacaacc
     2641 tttgcgctgt gacagggaat gcagccccta acgatggtgc aggaaccaac gatattgatg
     2701 atggaaaaac tactttgctc tcacctaact ttgatctttc ggcttactcc aatcctgcca
     2761 tttcctactg gcgttggtac accaacgatc agggagccac accgggcaca gactattggc
     2821 aggtttctgt gagcaatgat ggcggaacaa cttttgtgcc tatcgaaaac tgtattgttt
     2881 ccgatcatag ctggagacgt tttgcatttc gtgttgcaga ctatgtgcag ccaacagcca
     2941 acatgcgact gaaatttatt ggtgaagatg caaacgatgg aagtctggta gaaggcttgc
     3001 ttgatgatct tgaaatctgg agtgcagagc ctgtaggtat ttctgaaaca ccaatgttat
     3061 cgtggtatgc ctacccgaat cctgccaatg aacaacttaa tttcggatgg aaaggaaatg
     3121 gaacaacagt tgatgtgaaa ttattcaaca ccgttggaca aatagtttat cagaatcagt
     3181 tttcgggcaa cac
//
LOCUS       DAOOOL010000223         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3634_length_3193_cov_115.476737, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000223 DAOOOL010000000
VERSION     DAOOOL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_3193_cov_115.476737"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2648
                     /locus_tag="PKU82_11835"
     CDS             <1..2648
                     /locus_tag="PKU82_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83596.1"
                     /translation="EKLNYLNEKKLQLEKQGSSEQTQALEIEKQIAQAENEKISEEAK
                     TKELENEIQSLKSELESSRKNYDQSVDELRQLNLHILQVRQSVNDHETFIAVRQTRIN
                     SLNDQMKRLGEQIDKDRISLQNLEGELSMLAVKKDQAEKESHDKKQSTLQLDSEITQH
                     ENAVKELREKLADERRNYEVKNNEYQLTRNLAEKMEGFPESIKFLKKNSDSFKDIPLL
                     SDIINCDEQFKVAIENYLEPFLSHFIVQSNEQAWNGLNKLSSEGKGRAHFFILDSFTN
                     YHQASTHQIDGCIAASSVIENDGRYHSLMNYLLANVYIAQNSDVATQANQNEIEGKII
                     LSPDGKFYRQRYSISGGSVGSYDGKRTGRLKHLEKLSAEIQQHQQQTEVFKIELQQTE
                     ELLSAGKTQAAENRKIISLTDSELSKAVLNINTLLSKKDFITSNCESNEKNLQKLQEE
                     LQLTQEQKNNAPGFSSMSLDEMKINLQKLTDQQREKQNQSGQLQKISQEASNAFNQKN
                     ITFLQQQNKVQNIIREIGYKNNQVVSLKSSVEKIKTEIEQLHEQINDTAQAGNSSETD
                     LKTQVEEKVLLESRLGESEKNYFEAKGKIDQFDKQIAETRRKREMADELMHALHNEVS
                     DVNLKMTSLKERLSVEFSLEVEDIVSQEANPEWNEEDLKARNEKLGGQLKNFGPINPM
                     ALDSFKEIKERYDFIIKEQDDLKKAKDALLQTIAEIDNSAKEKFSEVFNQVRNNFINV
                     FRSLFTADDNCDMILADTNNPLESDIEIIAQPKGKKPLSIQQLSGGERTLTATALLFG
                     LYLVKPAPFCIFDEVDAPLDDNNIDKFNNIIKKFSKDSQFIIITHNKKTMAATDIIYG
                     ITMNEPGVTAVVPVDLREYDETLVE"
     gene            complement(2663..2914)
                     /locus_tag="PKU82_11840"
     CDS             complement(2663..2914)
                     /locus_tag="PKU82_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83597.1"
                     /translation="MKKFLNRFLMLLMLVISTSVVFAQSKATKAQRKAEKKKEMEKKK
                     VARAEEKARKRHMSIQTKEVRKRMKKNKKKSARETFRRE"
     gene            2987..>3193
                     /locus_tag="PKU82_11845"
     CDS             2987..>3193
                     /locus_tag="PKU82_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83598.1"
                     /translation="MRLKNIILISTLIFLISGCRKKDNGIVPYAYVNVVFYANDPQLV
                     QLNAVGNYIYYPAGYKGIIIYRRSI"
BASE COUNT         1185 a          540 c          585 g          883 t
ORIGIN      
        1 ttgaaaaatt aaattatctt aatgagaaga agctgcaatt agaaaagcaa ggttccagtg
       61 aacaaacaca ggctttagaa attgagaagc agatagctca agctgaaaat gaaaaaatta
      121 gcgaagaagc taaaaccaag gaattagaaa atgaaataca atctctaaaa agcgaactgg
      181 aatcatcacg caaaaactat gaccaatctg ttgatgaact ccgacaatta aatctccata
      241 tattgcaggt taggcaatct gttaatgatc atgaaacatt tattgctgta aggcaaacac
      301 gcatcaacag cctgaacgat cagatgaagc gattaggtga gcagatagat aaagacagaa
      361 tatcacttca gaatcttgaa ggagaattat caatgcttgc tgtaaaaaaa gatcaggcag
      421 aaaaagaatc gcatgataaa aagcaatcca cacttcaact tgattctgaa ataacacaac
      481 atgaaaatgc cgttaaagaa ctgcgcgaaa aactagctga tgaacgaaga aactatgaag
      541 taaaaaataa cgaatatcaa ctgacaagaa atcttgccga aaaaatggaa ggctttcctg
      601 agtcaattaa gttcttaaag aaaaacagcg attcgtttaa agatattcca ttactaagtg
      661 atattatcaa ttgcgatgag caatttaaag ttgcaataga aaattattta gaacctttcc
      721 tgagtcattt tattgttcag tcgaacgagc aagcatggaa cggactcaac aaactaagtt
      781 cagaaggtaa aggtcgtgca cattttttca ttttagattc ttttacaaat tatcatcagg
      841 catcaactca tcaaattgac ggttgtattg ctgcatcatc tgtaattgaa aatgatggac
      901 gctatcattc tctgatgaat taccttttag ccaacgtata tattgcacaa aacagcgatg
      961 tagctacaca agcaaatcaa aatgaaattg aaggaaaaat aattctttca cctgacggta
     1021 aattttacag acaacgttat tctatcagtg gtggttctgt aggcagttat gatggcaaac
     1081 gaacaggacg actgaaacac cttgaaaagc tatcagcaga aatacaacag catcaacaac
     1141 aaacagaagt attcaagatt gaattgcagc aaactgaaga gttgttatca gccggtaaaa
     1201 cacaagccgc agaaaaccgt aaaataattt cccttacaga tagtgaactt agcaaggcag
     1261 tattaaacat aaataccttg ttaagtaaaa aagatttcat tacttctaat tgtgagagta
     1321 atgaaaagaa tctccagaaa ttgcaagaag aactccagtt aacacaagag caaaaaaata
     1381 atgctcccgg gtttagcagt atgtcgcttg atgagatgaa aataaatctg caaaaactta
     1441 ccgatcagca aagagaaaag caaaaccagt cagggcaatt acaaaaaata tcgcaggaag
     1501 cctccaatgc attcaatcaa aaaaacatca cttttcttca gcaacagaac aaagtacaaa
     1561 acatcattcg cgaaattggc tacaaaaaca atcaggtagt ttctttaaag tcatcagtag
     1621 aaaaaatcaa aactgaaata gaacaacttc atgagcagat aaatgataca gcacaggcag
     1681 gtaactcttc tgaaactgac ttaaaaacgc aggtagaaga aaaagtattg cttgaaagtc
     1741 gcttaggaga aagtgagaaa aattattttg aagcaaaggg caagattgat cagtttgaca
     1801 aacaaattgc agaaaccaga cgcaagcgcg agatggcaga tgagttgatg catgctttac
     1861 acaatgaagt tagcgatgtg aatctgaaaa tgacttcatt gaaagaaaga ttgagtgtag
     1921 aattttcact tgaagttgaa gatattgtgt cgcaggaggc taatccggaa tggaatgaag
     1981 aagatttaaa ggcaaggaat gaaaaattag gtggtcagtt gaagaacttc ggaccgatta
     2041 atccaatggc tcttgattcg tttaaagaaa ttaaggaacg ttatgatttc atcataaaag
     2101 agcaggatga tttaaaaaag gcaaaagacg ctttactgca aaccattgca gagattgaca
     2161 attctgctaa agagaaattc agtgaagtgt ttaatcaggt tcgtaataac tttatcaatg
     2221 ttttcaggtc attgtttact gctgatgaca attgcgatat gattttggca gacactaaca
     2281 atcctttaga atcagatatt gaaattattg cacaacctaa aggcaaaaag ccactttcaa
     2341 ttcagcaact ttcaggagga gaaagaacac ttacggcaac agctttgctc tttggattgt
     2401 atttagtaaa gcctgcacct ttttgtatct tcgatgaggt tgatgcgcct cttgacgata
     2461 ataacattga taaattcaat aatatcatca agaagttttc taaagattcg cagtttatta
     2521 tcatcacaca caataaaaag acaatggctg caacggatat tatttatgga ataaccatga
     2581 atgagcccgg agttactgca gttgttcctg ttgatttacg agagtatgat gagactttag
     2641 tagagtaaac tgataaaaaa aattactcgc gtctgaaagt ttcgcgcgct gatttctttt
     2701 tgttcttctt catacgtttg cgcacttcct tagtctgaat actcatgtgg cgtttacgcg
     2761 ccttctcctc tgccctggca actttcttct tttccatttc ctttttcttt tcagcctttc
     2821 gttgcgcctt ggttgctttg ctttgtgcaa acacaacaga tgtacttata acaagcatca
     2881 acaacatcag aaatctattc agaaactttt tcatattaac taaacttcag ttttaacgat
     2941 tgtagaacaa ttatgttgta attttggaag attaacttca aaggtaatgc gtttaaagaa
     3001 tataattctg atttcaactc taattttttt aatatccggc tgccgtaaaa aagacaatgg
     3061 cattgtgcct tatgcttatg tcaatgtagt tttttatgcc aacgacccac aattagtcca
     3121 actgaatgca gttggtaact atatttatta tccggcagga tacaagggaa ttattattta
     3181 cagacgcagt att
//
LOCUS       DAOOOL010000224         3180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3660_length_3180_cov_85.811840, whole genome shotgun sequence.
ACCESSION   DAOOOL010000224 DAOOOL010000000
VERSION     DAOOOL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_3180_cov_85.811840"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKU82_11850"
     CDS             <1..227
                     /locus_tag="PKU82_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOZ83599.1"
                     /translation="FAPYVSDFINKYPTLKMLALAFLVVIGVILVVEALHIHFDKSYV
                     YVALGFSLLVEMLNIRMRSLSHRKKVDQQK"
     gene            224..1513
                     /locus_tag="PKU82_11855"
     CDS             224..1513
                     /locus_tag="PKU82_11855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ83600.1"
                     /translation="MSTSIGFDSLKLNRQLLNAIGELGYETPTEIQQKVIPPALSGQN
                     IIGIAQTGTGKTAAFILPLLRTLNYAQGNEPRALILAPTRELVIQIGAVANQMGKYVD
                     LRIVTVYGGKGFSDQKKKLEAGCDIVVATPAQAMELYLSGHLILKKIKHFVMDEAERL
                     MDFGFTGQIYSLLEVLPRKRQNMLFSATFSERVKKISDDFMEFPVFIDVVPQVKTAAK
                     IEQYLYTVNSFQTKLNLLNFLLSDKENFNKVIIFCKSKKNANRIAEVIQMNYGADGIN
                     VLHGDKTQQTRLNAINRFREEEIRLLIATDVAARGIDVPGVSHVINFDVPLVYEDYIH
                     RIGRTGRAFASGLSITFVEPQDEWHIKKIQKLAGISIKRLNIPESVEVIQPGREETQL
                     RDMEIDRQKRKDNPEFKGAFHERKNKSGKKGRHAGRK"
     gene            1452..2390
                     /locus_tag="PKU82_11860"
     CDS             1452..2390
                     /locus_tag="PKU82_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOZ83601.1"
                     /translation="MHFTSGKTNQARKADMQAGSKVMRWVYLLVLASMWGSSFILMKR
                     GLNSFSANQVAAIRMFISCMVMLPFIASHLKDIPKKLWLFVMATGLLGNGIPAYLFTH
                     AETGISGSMAGMLNSLTSMFTLVVGSIFFATQFSKRHLLGVIIGFAGAASLIWLNADD
                     AQSAEPWKGVYVLIATVCYAFSVNILRNKLASINAVTLTGAALLFAGIPSGIYLFSTD
                     FVSRFDTEPLAWNSFFYIALLAIMSTAISTVLFNHLIRISSAIYASSVTYLIPFVAVL
                     WGLYDNETFNMLYLLALLVILTGIYLTNYQKRVGKF"
     gene            complement(2614..2904)
                     /locus_tag="PKU82_11865"
     CDS             complement(2614..2904)
                     /locus_tag="PKU82_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83602.1"
                     /translation="MDYKDCPHYSEILTMLTDGKDLEDISFEIDEVQDCKECFGPNGV
                     CQLWVMLAFVEDMKPQMNIEDSKATIDNLDEQIEKIALRLIRALKSTNSDNN"
BASE COUNT          986 a          536 c          657 g         1001 t
ORIGIN      
        1 cttttgctcc ttatgtgagt gattttatca ataaataccc cacattaaaa atgctggcat
       61 tggcatttct agtagttatt ggagttatac ttgttgttga agcattgcac attcacttcg
      121 acaagagtta tgtttatgtg gctttaggtt tttcgttgct ggtagaaatg ctcaacatca
      181 gaatgcgtag cctgagtcat cgcaaaaagg ttgaccaaca aaaatgagta cttcaatcgg
      241 ttttgattcg ttgaaattaa accgccagtt attgaatgca attggagaat taggctatga
      301 aacgcctaca gaaattcagc aaaaggtaat acctcctgca ctttctggac agaatataat
      361 tggaatagca caaaccggaa caggaaaaac agcagcattt attcttcctc ttcttcgtac
      421 tttaaattat gctcaaggca atgagccaag agcgctcatc cttgctccta cccgtgagtt
      481 agtgattcaa attggtgcgg ttgcaaatca aatgggtaaa tatgttgacc taagaattgt
      541 gactgtttat ggtggcaaag gtttttctga tcagaagaaa aaacttgaag caggttgcga
      601 tattgttgtt gccacgcctg cacaggcaat ggaactttat ctttcaggtc atcttattct
      661 aaaaaaaata aaacactttg taatggacga ggccgaaagg cttatggact ttggttttac
      721 aggacagatt tacagcttac tagaagtgtt gcctcgtaaa aggcaaaaca tgttgttttc
      781 tgcaacattc tccgaaaggg taaaaaaaat atcggatgac tttatggagt ttcctgtttt
      841 tattgatgtt gttccgcagg ttaaaacagc agcaaaaatt gaacagtatt tatataccgt
      901 aaacagtttt cagacaaaat tgaatctgct gaacttttta ctttcagaca aagagaattt
      961 taataaagta atcattttct gcaagtcgaa aaaaaacgct aaccgcattg ccgaagtaat
     1021 acagatgaat tatggagccg atggtataaa tgttttgcat ggcgacaaga cacaacagac
     1081 aagattaaat gccattaacc gtttcagaga ggaagaaata aggttattga tagctactga
     1141 tgtggctgca cgaggtattg atgttcccgg agtttcgcat gtaataaatt ttgatgtgcc
     1201 tttggtttac gaagattaca ttcatcgtat tggccgtacc ggtcgtgcat ttgcatcagg
     1261 actttctatc acttttgttg agccgcaaga cgaatggcat ataaaaaaaa tacagaagct
     1321 tgcaggcatt tccataaaac gacttaacat tcctgagtct gttgaagtca tacaacccgg
     1381 aagagaagaa acgcagcttc gcgatatgga aattgaccgt cagaaaagaa aggacaatcc
     1441 ggaatttaaa ggtgcatttc acgagcggaa aaacaaatca ggcaagaaag gcagacatgc
     1501 aggcaggaag taaagtaatg cgttgggttt acttgctggt gcttgcatct atgtggggca
     1561 gttcatttat tttaatgaaa cgcggactga atagtttttc tgcaaatcag gttgctgcaa
     1621 taagaatgtt tatttcatgt atggtgatgc ttccatttat tgcgtcacat ttaaaggaca
     1681 taccaaaaaa actgtggtta tttgttatgg caacaggctt attaggaaat ggtattcccg
     1741 cctacctttt tactcatgca gaaacaggca tcagtggttc tatggccggg atgttaaact
     1801 cattaacctc tatgtttact cttgtggtgg gaagtatatt ttttgcaact caattcagta
     1861 aaaggcattt attaggagtg ataattggat ttgcgggagc agcctcattg atttggctca
     1921 atgcagacga tgcgcaaagt gcagaaccat ggaaaggtgt ttatgttctt attgcaacag
     1981 tatgttatgc atttagtgta aacatcctca gaaataaatt agcatcaata aatgcagtta
     2041 cactaacggg cgctgcattg ctttttgcag gaataccaag tggcatctat cttttttcaa
     2101 ctgattttgt ttcgcgtttt gatacagagc ctttggcatg gaacagcttt ttttacattg
     2161 cattgcttgc cattatgagc actgctatat caacagtttt gtttaatcat ttaatcagaa
     2221 tttcttctgc aatctatgct tcatcagtaa cgtatctgat tccatttgtg gctgtgcttt
     2281 gggggctgta cgataatgaa acgtttaata tgctttatct gctggcacta cttgtaatac
     2341 taacaggaat ttacttaacc aattatcaaa agagagttgg aaagttttga aagttgaaag
     2401 ttttgaaagt tgaaatttaa atcacactgt caggggcagt gcgacatgat aggagaaaat
     2461 aaataaaaaa cgtattaacc cttcaatgta tttttattat ttcaaagtca cgtgaaatgc
     2521 gggagacttt gaaagtacgg aatagcaaat tttatatgcg actggaagtc gtaagagtta
     2581 aatggcacga gcggaaagga cgctcgcgcc tacttagttg ttatcgctat ttgtagattt
     2641 taaagcacga attagtcgta atgctatttt ctctatttgc tcgtctagat tgtcaatggt
     2701 tgctttggaa tcttcaatgt tcatttgtgg tttcatatct tcaacaaatg ctaacattac
     2761 ccacaattgg caaaccccat taggtccaaa acattcttta cagtcttgga cttcgtcaat
     2821 ttcgaagcta atatcttcta aatcttttcc gtctgtcaac attgttagta tttcagaata
     2881 atgtgggcag tctttgtagt ccattgattt agtttttgtt gaagtttaaa cttgcccata
     2941 acggcagtct ttccgataac tatcggaatt aaaactattc attagctcaa aagtaaaaag
     3001 aaaaatgatt caagaaggca ttagcagtga actatgttgt attacaatta attgtttgtg
     3061 ttaaaacaca ctgtcagagg gggcgacatg acaggtgaaa ttaactgctg cttgaatgat
     3121 agatgggcac aagtccgaag acttgcgccc aatgtcatta agttaaggaa taagaaattg
//
LOCUS       DAOOOL010000225         3171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3685_length_3171_cov_46.323813, whole genome shotgun sequence.
ACCESSION   DAOOOL010000225 DAOOOL010000000
VERSION     DAOOOL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3685_length_3171_cov_46.323813"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..751)
                     /locus_tag="PKU82_11870"
     CDS             complement(185..751)
                     /locus_tag="PKU82_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HOZ83603.1"
                     /translation="MEERVKTDLSKFNPSEFDRGASSLKFFLWHWVNVIFFISPFCPS
                     SAIRCFLLRMFGARVGKGVVISKPGVNVKFPWRLTIGNHVWIGEKAWIYNLDQVTIGN
                     HVNIAQGAMILTGNHNYKSSKFETIFGPVTIEDGVFIGAKAIVCPNVTCYSHSILAAG
                     SVATSALKAYGIYQGNPAEYKKERVIQE"
     gene            complement(781..1341)
                     /locus_tag="PKU82_11875"
     CDS             complement(781..1341)
                     /locus_tag="PKU82_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HOZ83604.1"
                     /translation="MRFLTVFVICFVLSQTTNAQRELIEWNDSLSFKIEDYKGPITSP
                     DKSLHISTNCGLYCIPQIVGDTAILTIIAYMDKNKSWARKKDVTPALLEHEKGHFDLT
                     EVFARKMKKRVLALRTNKRTFIKDVQEVYDKTWEDLQKQHQAYDKATEFSNVEFAQRT
                     WQNYIKEHLVQYHFYASQTIRFPIKD"
     gene            1509..1805
                     /locus_tag="PKU82_11880"
     CDS             1509..1805
                     /locus_tag="PKU82_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOZ83605.1"
                     /translation="MFGKLAEAQKKMQEIKDRLETIIVTGESEQGKVIAEANGNNRIR
                     SITINESLFKVGDKNEVEELITVAVNRALEASEKLMQGEMAAASKDILPGFLQK"
     gene            complement(1815..2570)
                     /locus_tag="PKU82_11885"
     CDS             complement(1815..2570)
                     /locus_tag="PKU82_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83606.1"
                     /translation="MQMFAGSYYHCPMKYWSFKICAIAILCFFVDAIQAQTIHVVATV
                     KGNDATLPVVGLMIVNKSTGTGFFSPDNNFFSAVFNKSDTIMVNATGYSVKKICFKDS
                     LLRDTFRISIILEKPVVTLKPVTIIPPRELEVIQKDIEKLGYKKEDYMVTGIDAFESP
                     ITFLYQSFSRRERAKRDIAQKKNEDNKRKLLKELFRKYVDFDIMNLSEDQFDNFIDFI
                     NVDDNFLKNSTQYDFILFVKKKFEMYKALNRAK"
     gene            complement(2640..>3171)
                     /locus_tag="PKU82_11890"
     CDS             complement(2640..>3171)
                     /locus_tag="PKU82_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83607.1"
                     /translation="AVSNVMSNRATVSWNPQSGVSYVVVKYAPATSSQWKYAYVRASA
                     GQTIIRNLTPGVQYNIQLKSMCTYSNNSAYSAMERFTTGIARLGEATSELMINAYPNP
                     ADAQITYAFDAKKEGYFFIRVSDIAGRILLNDKKMASEGTNGGDIDLRMFTPGVYTLT
                     ITKGSEENHIRFEVNR"
BASE COUNT          989 a          611 c          542 g         1029 t
ORIGIN      
        1 cataaacatg gttttttggt ttataattat gtcctgcatt tattcaaatg caaaccaaaa
       61 ttaagcccat aaaatagaaa ttccaaattt atctgagaaa aaaactcttt tgttatgata
      121 aaattttaaa taaaatgata tgttccatca ctcaatcata ataaaaacat aatgttcctg
      181 ttctttactc ctgtatcact ctttcctttt tgtattcagc agggttgccc tgataaatac
      241 cataagcttt aagtgctgat gtagctacag agcctgcagc cagtatgcta tgcgaataac
      301 aagttacgtt tgggcaaaca atagcctttg cgcctataaa aacaccatct tcaatagtaa
      361 caggtccgaa aatggtttca aacttcgaac ttttataatt gtgattcccg gttagaatca
      421 tagcaccctg tgcaatgtta acatgattgc caatagttac ctgatcgaga ttataaatcc
      481 aagctttttc gcctatccac acatgattgc caatggtcag tcgccagggg aattttacat
      541 taacaccggg cttgctgatg actacaccct tccctaccct tgcaccaaac atgcgcagca
      601 agaaacagcg tatggcactt gaaggacaaa aggggctgat gaaaaaaatt acattgaccc
      661 aatgccacag aaaaaatttt aacgaagatg cgccacggtc aaattctgat ggattaaatt
      721 ttgacaaatc ggtctttaca cgttcttcca ttggccaaaa ataaaaaatg aaacaatgtg
      781 ttaatccttt atcggaaatc gtatggtctg gctggcataa aaatgatatt gcaccaaatg
      841 ttctttaata taattctgcc aggttcgctg tgcaaattct acattagaaa actctgttgc
      901 cttatcgtaa gcctgatgtt gcttttgcaa gtcttcccat gttttgtcat acacttcctg
      961 cacatcttta atgaaagtac gtttattcgt ccttaaagcc aataccctct ttttcatttt
     1021 acgggcaaaa acttctgtta aatcaaaatg acctttttca tgttctaaca atgctggcgt
     1081 tacatctttt ttgcgtgccc acgatttgtt tttatccatg tatgcaataa tggttagaat
     1141 ggctgtgtcg ccaacaatct gtggaataca atacaagccg cagttggttg aaatatgcag
     1201 gcttttatct ggcgatgtta ttggaccttt ataatcttca attttaaatg ataaagaatc
     1261 gttccactct atcaattcgc gttgggcatt ggttgtctga cttaaaacga agcaaattac
     1321 aaatacagtc aaaaagcgca tactttctaa aattttagat tcttatacgg ccgttctcct
     1381 gtttatttta cattaatatc tctataaacc ttacatcacc cttactgttt tttaaaattt
     1441 gtcggatgtt tttttggcga gaattcttgt gtatgactaa ctttgcagtc attaaaaacc
     1501 gaataaatat gtttggaaag ttagcagagg cacaaaagaa aatgcaggag ataaaagaca
     1561 gattggaaac aataattgtc accggagaat cggaacaagg caaggtgata gccgaagcca
     1621 atggcaataa ccgcatccgt tcaataacta tcaatgaatc gttgtttaaa gtaggcgata
     1681 aaaacgaagt tgaagagttg ataaccgttg ctgtaaaccg tgcgcttgaa gcttcagaaa
     1741 aattgatgca aggcgaaatg gctgcagcat caaaagatat tctgccggga tttttgcaga
     1801 agtagttaca gttattattt cgctctattt aaagccttat acatctcgaa tttcttcttt
     1861 acgaaaagta taaaatcata ctgcgtagaa tttttcagaa aattatcgtc aacattaatg
     1921 aagtcaataa agttgtcaaa ctgatcctca cttaggttca taatatcaaa gtcaacatat
     1981 ttcctgaaca attctttgag aagttttctc ttgttgtctt cgtttttctt ttgtgcaatg
     2041 tctcgtttgg ctctctctct cctgctgaaa ctctgataca aaaaggtaat cgggctttca
     2101 aatgcatcaa ttcccgtaac catgtaatct tcttttttat agcccaactt ttctatgtct
     2161 ttctgaataa cttcaagttc gcgtggagga attatagtta caggtttcag tgtaacaaca
     2221 ggtttttcga gtataatgga tattctgaaa gtgtctctga gtaatgaatc tttaaaacat
     2281 attttcttaa cggaatagcc tgtggcattt accattatag tgtccgactt attaaataca
     2341 gcagaaaaaa aattgttatc cggagaaaaa aagccagttc cggttgactt gtttacaatc
     2401 atcaatccta ctacgggtaa tgttgcatca ttgcctttaa cagttgcaac tacatgaatg
     2461 gtttgcgcct gtatggcatc aacaaaaaag caaagaatgg caatggcaca aatcttaaaa
     2521 ctccaatact tcatagggca atgataataa cttcctgcaa acatctgcat gaagattggt
     2581 ataaaaaaag aggaagcctt ttcaagcctc ctctcatttt gaagttactg tttctgtttt
     2641 tagcgattca cttcaaatct tatatgattc tcttcactac ctttagtgat agtaagtgta
     2701 taaacaccgg gagtaaacat tctcagatca atgtcgccac cattggttcc ttcagaagcc
     2761 atcttcttat cgttcaacaa aattcgtcct gcaatatcac tcactctgat aaagaaataa
     2821 ccttctttct ttgcatcaaa ggcataagta atttgagcat cagcaggatt tggatatgca
     2881 tttatcatca gttcggaagt agcttctccc aatcgtgcaa tacctgttgt gaaacgctcc
     2941 attgcgctat aagctgaatt gttcgaatag gtgcacatag attttagctg aatattatat
     3001 tgtactccgg gagtaagatt tctgataatt gtttgtccgg ctgatgctct tacataggca
     3061 tatttccact gactgcttgt tgcaggtgcg tattttacta caacataaga aacacctgat
     3121 tgtggattcc aacttacagt agctctgttt gacattacat ttgatacagc a
//
LOCUS       DAOOOL010000226         3091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3816_length_3091_cov_40.181489, whole genome shotgun sequence.
ACCESSION   DAOOOL010000226 DAOOOL010000000
VERSION     DAOOOL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_3091_cov_40.181489"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..247)
                     /locus_tag="PKU82_11895"
     CDS             complement(17..247)
                     /locus_tag="PKU82_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOZ83608.1"
                     /translation="MISVIPIDQFVINHASHEVLLDTRSPAEYEHAHFPGAVSFPLMT
                     NEQRAVIGTIYKQEGKDAAVLKDEYDLIQKMN"
     gene            complement(261..695)
                     /locus_tag="PKU82_11900"
     CDS             complement(261..695)
                     /locus_tag="PKU82_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOZ83609.1"
                     /translation="MKAITFILAVVMLTSSSCLNLKMPQVTGVSKLRLSDITKDTRIQ
                     FDVGIRNPNTFGVTLKSMKAELFLADSSIAGIGVDRKTRLAANQQVDMPFSVQPKLTA
                     LPQLGILGITQLFKKDNKKISLRGELKVRKFIFTKKVKFSIP"
     gene            846..1028
                     /locus_tag="PKU82_11905"
     CDS             846..1028
                     /locus_tag="PKU82_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOZ83610.1"
                     /translation="MNESKRLVKGEKKLFGVCSGIANYFEADPTLIRLLFVIAVFGFG
                     TGVLLYIILAIIMPEK"
     gene            1186..1989
                     /locus_tag="PKU82_11910"
     CDS             1186..1989
                     /locus_tag="PKU82_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOZ83611.1"
                     /translation="MSSSNNSGGGFKSIFAAIVIPAAILVAILIYAYILGNPANFMEN
                     DPNNHPQNYLGIIHKGGVIVPILISLLLIVITISIERMMTIGASKGKSSVDSFVGKIR
                     SLLSNGDLNTAIAECDKQKGSIANVVKAGIESYRKMQGETSLDKERKTAAIQKELDEA
                     TTLELPMLSKNLVIISTVASVATLMGLLGTVIGMIRAFAALAQAGAPDAIALATGISE
                     ALINTALGVGTSALAIIMYNYFTNKIDSLTYGIDEASYSIVQSFTANTK"
     gene            2020..2667
                     /locus_tag="PKU82_11915"
     CDS             2020..2667
                     /locus_tag="PKU82_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOZ83612.1"
                     /translation="MPKIKIPKKSPSLDMTPMVDLAFLLVTFFMLTTKFRPDEPVIVD
                     TPASVSTKILPENTLQVTIDSAGRVFFNIEGQNVRRNLMTRISEKYKFQLTEADLKKF
                     SLMSTFGMPAEKIKAYLEAPDSERKKMDKATGGIPIDTLNNQLADWIVFGWTAAITDE
                     AYEARKQAGNELRFAIKGDGRVDYTYIKRVIEIFQQNKINRFNLITNLQATPVEQ"
     gene            2674..>3091
                     /locus_tag="PKU82_11920"
     CDS             2674..>3091
                     /locus_tag="PKU82_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOZ83613.1"
                     /translation="MAEVNTDQGGGKEKGGKHSKVRAKKASTHIDMTPMVDLAFLLLT
                     FFMLTTTFSKPKTVEINMPLKDGEPTKVNNAITVLLSDKDRVFWYFGEFKPGETQLNK
                     SDFSDNGIRKILLDKNAVAHAEVKKLEDELKSRQIAD"
BASE COUNT         1043 a          556 c          577 g          915 t
ORIGIN      
        1 aaacttgttg ccatgattag ttcatttttt gaattaaatc atattcatcc ttcaaaacag
       61 cagcatcttt accttcctgt ttatagatgg ttccaattac tgcacgttgc tcattagtca
      121 ttaatggaaa tgaaaccgct cccggaaaat gtgcatgctc atactctgca ggactacgtg
      181 tgtcaagcaa tacttcatgc gatgcatgat taattacaaa ctggtcaatt gggattactg
      241 agatcaaggg tggagtgata ctatggaatg gaaaacttaa cctttttggt aaaaataaac
      301 tttctgacct ttaactctcc tctaaggctg atttttttat tgtctttctt aaaaagttgt
      361 gttattccca gaatacccaa ttgtggcaat gctgtaagtt taggttgcac tgaaaaaggc
      421 atatcaactt gctgattggc tgctaacctt gtctttctgt caacaccaat tcctgctatg
      481 gaactgtcgg caagaaacaa ttctgctttc attgatttta aagttacccc aaatgtattg
      541 ggattacgga tgcctacatc aaactgtatt cgtgtgtctt tagtgatatc gcttaatctt
      601 aatttactta ctccggtaac ctgaggcatc ttgaggttaa gacagctact tgaagttaac
      661 attacaactg ctaagataaa tgtaattgct ttcatacaga aattttaaaa taaacgccaa
      721 aagataaaaa taatgtaaaa aatatggagt tatttataaa gatgattaaa atgaacttgc
      781 aaattaaaca atttatttat ttttgaccgc gagaataaca accattatct atctgaacca
      841 acagcatgaa cgaatctaaa cgattagtta aaggagagaa aaaactattt ggtgtatgca
      901 gtggcattgc caattatttt gaagccgacc ctacattaat tcgcttgctt tttgttattg
      961 cagtttttgg atttggaaca ggtgtgttgt tatacatcat acttgcgata attatgcccg
     1021 aaaagtaata ttttatgatt tctgaaaaaa caatacaccc aaccgtaata ttcatgaatt
     1081 caaaaataaa gcggcaaatt gtcaagtttt cattatactt gccctccatt ttgacaaata
     1141 attaaacata gataatcaaa tttaaaactt aaattaaaaa cgaacatgag ttcatctaac
     1201 aattcaggcg gtggtttcaa atccatcttt gcagcaatag taattccggc agcaatcttg
     1261 gttgccatcc taatttatgc ttatatatta ggtaatccgg caaactttat ggaaaacgac
     1321 ccgaataatc atccacagaa ttatctggga attattcata aaggaggtgt aattgtgcct
     1381 attctgatta gcttattatt aattgtaatc accatttcta ttgagcgtat gatgactatt
     1441 ggtgcttcaa aaggcaaatc atctgttgac tcattcgtag gcaaaataag aagtttgcta
     1501 agcaatggcg atttaaatac agccattgca gaatgcgaca aacaaaaagg ttctattgcc
     1561 aatgtagtaa aagcaggcat tgagtcttat cgcaaaatgc agggcgaaac ttctttggat
     1621 aaagagcgca aaacagcagc tatccagaaa gagttagatg aagccacaac tttagagtta
     1681 ccaatgcttt caaaaaatct ggttatcatc tctacagtag catctgtagc tacattgatg
     1741 ggactattag gaacggttat cggtatgatc agggcgtttg ctgctcttgc acaagccggt
     1801 gcacctgatg ccatagcgct tgcaaccggt atttctgagg ccttgattaa tacggcactt
     1861 ggagtaggaa cgtcagcttt agcaatcatc atgtacaact atttcactaa taagattgat
     1921 tcgttaactt atggaataga tgaggcaagc tatagcattg ttcaatcgtt tacagcaaac
     1981 actaaataaa ttagcgtaat taattatcag cattagataa tgccaaaaat taaaataccc
     2041 aaaaagagtc catcgttaga catgacaccc atggtggatc tggcgttttt gctggttaca
     2101 ttctttatgt taactaccaa attccgtccg gacgaaccgg tcattgttga cacaccggca
     2161 tctgtttcaa caaaaatttt acctgaaaat acattgcagg taacaattga ttcagctggc
     2221 cgtgtgtttt ttaatattga aggacaaaat gtgcgcagaa acctgatgac aagaatcagt
     2281 gagaaataca agttccagct tacagaagcg gatctgaaaa aattctcctt gatgtcaaca
     2341 tttggaatgc ctgccgaaaa aataaaagcc tatcttgaag cacccgattc cgagcgcaaa
     2401 aagatggata aggccacagg cggaattccg atagatactc tcaataatca acttgccgac
     2461 tggatagttt ttggctggac agcagccata actgatgaag cttatgaagc ccgtaaacaa
     2521 gcaggcaatg aactgcggtt tgccataaaa ggtgacggca gggttgatta tacttacatt
     2581 aaacgtgtga ttgaaatttt tcagcaaaac aaaatcaacc gttttaattt gataacaaat
     2641 ttacaagcaa caccagtaga acaataagaa aaaatggctg aagtaaatac cgaccagggt
     2701 ggtggtaaag agaaaggcgg gaaacatagt aaggttcgcg ccaagaaagc ctccactcac
     2761 attgacatga cacctatggt ggatctggcg tttctgctac tcaccttttt tatgcttact
     2821 accactttca gcaaaccaaa aacggttgaa attaatatgc cgttaaaaga tggtgagcca
     2881 acgaaagtta ataatgcaat aactgtacta ttgagtgata aagacagagt gttttggtac
     2941 ttcggtgaat ttaaacccgg agaaacacaa ctgaacaaat ctgatttttc tgataatggt
     3001 attcgcaaaa tattattaga caaaaatgct gttgcacatg cagaagtaaa aaagcttgaa
     3061 gatgaattga aaagtcgtca gatagcagac t
//
LOCUS       DAOOOL010000227         2997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_3988_length_2997_cov_56.667573, whole genome shotgun sequence.
ACCESSION   DAOOOL010000227 DAOOOL010000000
VERSION     DAOOOL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_2997_cov_56.667573"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..568)
                     /locus_tag="PKU82_11925"
     CDS             complement(194..568)
                     /locus_tag="PKU82_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOZ83614.1"
                     /translation="MRTTILRFTFLVSSILLFSFSSHAQESKTDTIKVYGNCNMCKEK
                     IEGSLKEKDGITAKNWDKKTKLLAVTYNPSKITKEQIGQKIADVGYDNQYATATEKVY
                     KKLHSCCQYDRPKQPAGDEKNK"
     gene            complement(724..1947)
                     /gene="lhgO"
                     /locus_tag="PKU82_11930"
     CDS             complement(724..1947)
                     /gene="lhgO"
                     /locus_tag="PKU82_11930"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOZ83615.1"
                     /translation="MSSQAEYDIVVIGGGCVGMASAYKLNLRFPHLKIAVLEKEDRLA
                     AHQTGRNSGVIHSGLYYKPGSYKAKNCVEGRRELVAFCKEHQVAHDVCGKIIVATDEK
                     ELPHMNRVFNNGLQNGVEGIEKINAQQIKDIEPFCTGIEGIWVPCTGIVDYVGLTNKL
                     AELIEQKFPGSKVYTGHEVTAFEKEDGNTTVVTNKQKFKTKHLISCTGLFSDRMAKAD
                     GVNPNMQIVGFRGDYYDLTEKGMHKVRNLIYPVPNPEFPFLGVHFTRMIQHGVECGPN
                     AVFTFKREGYGKTDFDLKDTLQALAYIGTWKLFFKHWRFGLDEYKRAFSKKLFLERLQ
                     RLIPSLEMDDITPGRAGVRAMALDANGNMIDDFKIEYSGNAIHVLNAPSPAATASLAI
                     GTAVMEMAEKHFQIH"
     gene            complement(2086..>2997)
                     /locus_tag="PKU82_11935"
     CDS             complement(2086..>2997)
                     /locus_tag="PKU82_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83616.1"
                     /translation="ALIIAGEYNTKSNAQGEFILRVPTGFYKLKIQTGSGHVFKSMVG
                     VEVKANDTLRLSDSESLLQQIKQLAFILGAYDRIETIIIDSLGYAATAISVNDLDNLS
                     YMQSFGGIFFDCGKSGSLDSLKYTNISNYLVNGGSIYASDWAIEYLTGDGSWRPALGS
                     NTLNVSASKNQSHEAGYSTMATCLSSEVGGFIPDSALCASKSGAIGLITNALINDPDI
                     VTLLGKDSMDIEYNLGSWEKISHVDIPFTTMIEKNNPGPGPLAVSCDLNGTAFGGRII
                     YTTFHNHPQGNISTDVKLVLQHFILSL"
BASE COUNT          888 a          585 c          508 g         1016 t
ORIGIN      
        1 aagatgcaca aggcattacc caacataaaa caattacttt tccggaattt aattcagtaa
       61 agagattgtg cgaattgccg ttacagtcgt ttgctgtaaa gtctgttgct gtttgtgcca
      121 tacctaaaga tgttatggca aaaagaataa gtgtaaggag ttgctttttc attttgatat
      181 gtttgtttag tgtctattta tttttttcat caccggcagg ttgtttaggc ctgtcgtact
      241 gacaacatga atgcagtttt ttatatactt tctcagtagc tgttgcatac tgattatcgt
      301 agccaacatc tgcaattttt tgtcctattt gttcttttgt gatttttgaa ggattataag
      361 ttactgccag tagtttggtt ttcttatccc agttttttgc agtaatgccg tctttctctt
      421 ttaaggagcc ttcaattttt tctttacaca tattacagtt accataaact ttaatagtgt
      481 ctgttttact ttcctgtgca tggctgctaa agctaaacaa caagatgctt gaaactaaaa
      541 atgtaaaccg gagaattgta gtgcgcataa tgcttattaa ttagttaata attctctgca
      601 aaattaaaga ataaggtcaa tcaatgcgtt gggactaaaa tgataattgt catttgcggt
      661 aatgagtaat tttcctgaaa acagataggt ttatgtcagt aaaagtaact tttaaaatcc
      721 gctttaatga atctgaaaat gcttttctgc catttccata actgctgttc caatagctaa
      781 cgatgcagtt gctgccggtg aaggggcatt taaaacgtgt atggcattgc cgctgtattc
      841 aattttaaaa tcatcaatca tatttccatt tgcatcaagt gccattgctc taacacctgc
      901 acgtccggga gtgatgtcat ccatctccaa tgaaggaatc aatctttgca gtctttcaag
      961 aaataatttt ttggagaaag ctcttttata ttcatcaaga ccaaaacgcc agtgtttgaa
     1021 aaagagtttc catgttccga tatatgctaa tgcctgaagg gtgtctttta aatcaaagtc
     1081 agtcttacca taaccttcgc gtttaaaagt aaaaacggca ttcggtccgc actctacacc
     1141 atgctgaatc attcttgtaa aatgcacacc taaaaaagga aactcaggat taggaacagg
     1201 ataaataaga ttgcgcactt tatgcattcc cttttctgtc agatcgtagt aatcacctct
     1261 gaaacctaca atctgcatat taggatttac gccatctgct tttgccattc tgtcgctgaa
     1321 caaccctgtg caactgataa gatgcttggt tttgaatttt tgtttattgg taacaactgt
     1381 ggtgtttcca tcttcttttt cgaaggcagt aacttcatgg ccggtataaa ctttactacc
     1441 cggaaatttt tgttcaataa gttctgccag tttattagtc aacccaacat aatcaacaat
     1501 acctgtgcag ggcacccaaa tgccttcaat gccggtacaa aatggttcaa tgtcttttat
     1561 ctgttgtgca ttaatttttt caattccttc aacaccattt tgcaaaccat tattaaaaac
     1621 gcggttcatg tgtggcagtt ctttttcatc agtagcaaca ataatttttc cgcatacatc
     1681 gtgtgccacc tgatgttctt tacaaaaagc aacaagctca cgcctgcctt caacacagtt
     1741 ttttgcttta taggatcccg gcttatagta aagtcctgaa tgtattacgc ctgaatttct
     1801 tcctgtctga tgtgcagcaa ggcggtcttc tttttcgagc actgcaattt tcagatgtgg
     1861 gaaacgcagg ttaagtttat aggctgatgc catacctaca catcctccac caattactac
     1921 aatatcatat tctgcttgac tactcatgct tgttgtttaa actgccgcaa aggtagaaaa
     1981 gcagagagtt gattgatgcc aatcctttaa ataaaatcag agcaataata ttcgaaatga
     2041 ctccgacttg caaacaataa tttgtaaaaa agttgaatta ctctgctaca gactgagaat
     2101 aaaatgttgt aagactaact taacatctgt actaatgttt ccttgaggat gattatgaaa
     2161 cgttgtatat ataatacgac ctccgaatgc tgtaccgttt aggtcacatg aaacagcgag
     2221 cggtccaggt ccgggattgt ttttttcaat catggttgta aatggaatat ctacatgact
     2281 aattttctcc catgagccta aattatattc tatgtccata gaatctttgc ctaataatgt
     2341 cacaatatcc ggatcattaa tcagtgcatt tgttattaaa cctattgctc ctgactttga
     2401 tgcacacaat gcactatcag gaataaagcc acccacttct gatgataaac atgttgccat
     2461 tgtactgtac ccggcttcat gagattgatt ttttgatgca cttacattta atgtgtttga
     2521 acctaatgca ggtctccagc ttccatcacc ggttaggtat tctatagccc aatcagaagc
     2581 ataaatacta cccccattaa ctaaatagtt gcttatattg gtgtatttta aactgtctaa
     2641 tgaaccactt tttccacagt caaaaaatat tccaccaaaa ctttgcatgt aagaaagatt
     2701 gtctaaatcg ttaacagaaa ttgctgtggc agcataacca agcgaatcaa tgataatggt
     2761 ttctattctg tcataagctc ccagaatgaa agctaattgt ttaatttgtt gaagtaacga
     2821 ttctgaatca ctcaacctca aggtgtcatt agctttaact tccacaccaa ccattgactt
     2881 aaatacatgc ccactgcctg tttgtatttt gagtttataa aatcctgttg gaacacgaag
     2941 tataaattca ccttgtgcat tacttttagt attgtattca ccggcaatta ttaatgc
//
LOCUS       DAOOOL010000228         2965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4053_length_2965_cov_29.310309, whole genome shotgun sequence.
ACCESSION   DAOOOL010000228 DAOOOL010000000
VERSION     DAOOOL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4053_length_2965_cov_29.310309"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..535)
                     /locus_tag="PKU82_11940"
     CDS             complement(413..535)
                     /locus_tag="PKU82_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83617.1"
                     /translation="MHKADGGLMESTTDTNHKQADKQNSLLKDGNEYDGGENAC"
     gene            complement(725..1531)
                     /locus_tag="PKU82_11945"
     CDS             complement(725..1531)
                     /locus_tag="PKU82_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83618.1"
                     /translation="MAKQTGLVRYSGSMGGVRHFKIKGLSGDFAGLNGGPSGDQIKTA
                     PEFERTRENMNEFGGCATAGKSIRVGLSQLVKQMSDPQMTGRLTGVIKKINLEDQTEA
                     RGYRAILISQEKQYLTGFAFDKNVSLEGVFTAPYTLTSTPARDSGTFTVPAFNPANLV
                     NAPAGATHFRLIQALACVSDFAYNDQTGVYEPIDPANNELSDVQYSGYIDLFTPVAAP
                     TVITATLPGSPTLTADASVLQCIGIEFYQQVGPNYYLFNSGNALKIQDIF"
     gene            1620..2114
                     /locus_tag="PKU82_11950"
     CDS             1620..2114
                     /locus_tag="PKU82_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOZ83619.1"
                     /translation="MMQFKAYGQAIIKTHLKITKVVDGDGIFVMNIFNKQEEEIRFLG
                     IDAPEIKRSKKLQQDERETHLPGQLLLSLGHQSKTFLNSIAPVGTSVTIQMERPNGID
                     SFGRTLAYVFLADGTCLNELMISEGYAKAYSKYYCEALSDFQLINTFARNKGRGHYKI
                     VSVF"
     gene            2307..>2965
                     /locus_tag="PKU82_11955"
     CDS             2307..>2965
                     /locus_tag="PKU82_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOZ83620.1"
                     /translation="MCILILASCNSSKTEEIASTEMDTTHSCMNIPSRFGNDSSGNSI
                     QFNGDSSTNGMLLIPGGVFDMGADNEQASPDEYPKHEVQVSAFYMDETEVTNAQFKKF
                     VDATGYITTAEKKPDWEELKKTVPPNTSKPPDSVLVAASLVFRSSNGPVDLNDFNQWW
                     SWVNGASWKHPQGSGSTINGKENYPVVQVSWDDAMAYCKWAGKRLPTEAEWEFAARGG
                     L"
BASE COUNT          911 a          588 c          599 g          867 t
ORIGIN      
        1 tgaaaaaaca aacacaccag gtgaaacaaa ccagcaagaa acccaaatgc tgccatgtga
       61 atattaaaag aaggcgggca gaaatgtccg cttttcttac atccttcatg acctgttgaa
      121 ttgatacagc ttttgccaat gtgtttttta ttcagcaaaa gtagctacgg cagcccaccg
      181 cacaaccctg acaaaaaaaa caaaaagact acggcataaa cacagccccg gcacacagaa
      241 gccatctttt attccgtttc ctttttggtt tttttatctg ccttcgctga gagatgctta
      301 taaaaattca cagttatgca aaagcagctt cgtatcatca ccagtcgcag tttggcagtt
      361 tgtccattca gcagttcttt attcccggtt ccttcagttc cgcaggtagt tttcagcagg
      421 cattttcgcc gccatcgtat tcattaccgt cctttagtag gctgttctgt ttatcggctt
      481 gcttgtggtt ggtatctgtg gtgctttcca tcaggccgcc gtcagctttg tgcattgccc
      541 ttttaagggc tttgcacttt taccctgact tgtttttacc ctgactgcat ttcgaccata
      601 agccaaccat aacccgaggt taaaagcacc atgattcaac acacaatgat ttttttgaat
      661 tgcatggcac ataaaaaagc cccggaagaa tccagggctc acaacagagt agatatttca
      721 atccttaaaa aatatcctga atcttcaggg cattgcctga gttaaacaga tagtagttag
      781 gtcctacttg ctgataaaac tcaatgccga tacactgcaa cacacttgca tccgcagtaa
      841 gtgtaggtga tcctggcagc gtagctgtta taacagtagg agctgcaacc ggagtgaaca
      901 aatcgatgta gccggaatac tgcacatctg aaagttcatt gtttgccgga tcaattggct
      961 cataaacgcc ggtctgatca ttgtaagcaa aatcagaaac gcaagccaat gcctgaatca
     1021 aacgaaagtg agttgcacca gcaggtgcgt tcacaaggtt tgccggattg aatgcaggta
     1081 ctgtgaacgt accactgtca cgggcagggg tacttgttaa agtgtaaggt gcagtgaaca
     1141 caccttccag gctaacattc ttatcgaatg cgaagccagt caaatactgc ttttcctgtg
     1201 aaatcaaaat tgcccggtaa cccctggctt ctgtttggtc ttccaaatta attttcttga
     1261 ttacaccggt taatcttccg gtcatttgtg gatcactcat ttgtttcacc aactgactga
     1321 gaccaacacg aatggatttt ccggcagttg cacatccgcc gaactcgttc atattttcac
     1381 gagtacgttc aaactcagga gccgttttaa tttgatctcc agaaggtcca ccatttaaac
     1441 cggcgaagtc gccagataaa cctttgattt taaaatgtcg aactccgccc attgaaccag
     1501 agtaacgaac taaacctgtt tgctttgcca tgatattaaa ttttaatttt gaaaattcat
     1561 tttttcaata caaatttaca tcacaacgca ctgaaattca gttagttgaa tgaagcaata
     1621 tgatgcaatt caaagcatat ggacaggcaa ttataaaaac tcatcttaaa atcactaagg
     1681 tggttgatgg cgatggcata ttcgtcatga atatttttaa taagcaggaa gaggaaataa
     1741 gatttttagg tattgatgca cctgaaataa agagatcaaa aaaactgcaa caggatgaaa
     1801 gagaaacaca cttaccgggt caattgcttt tatcgttagg gcatcaatca aaaacatttt
     1861 taaatagtat agctccggtt ggaacttctg taacgataca aatggaaaga ccaaatggta
     1921 tcgattcatt tggcagaaca ctggcttatg tttttcttgc agatggtact tgcttaaatg
     1981 aattaatgat cagtgaaggg tatgcaaaag cgtatagtaa atactattgt gaagcattga
     2041 gtgatttcca attaatcaat acatttgcga gaaataaagg gagaggtcac tacaaaatag
     2101 tttctgtttt ttaagttgaa aagattttat ccttgtcttc ttttggttct gctttcgtgt
     2161 tgccctcttg ttgcccgacc tctcaaacat cagtctatac tgcattcttt actttttgta
     2221 tcggcaagta atcgcattat tgctaaatga ataaattgaa gaatatatta acgatcatat
     2281 gcaatagaaa ttacttttca gacttgatgt gtatattgat attggcttcc tgtaatagtt
     2341 caaaaacaga agagatagca tctacagaaa tggatactac ccactcttgt atgaatattc
     2401 cttcaaggtt tggtaacgat tcatccggta acagtattca gtttaatggc gattcttcta
     2461 caaatggaat gctgctgatt cccggtggtg tttttgatat gggtgccgac aatgaacagg
     2521 caagccctga tgaatatcca aaacatgaag tgcaggtgtc tgctttttat atggatgaaa
     2581 ctgaagtaac caatgcacag tttaaaaaat ttgtagacgc aacaggttac ataacaacag
     2641 cagaaaaaaa acctgattgg gaagaattaa aaaaaacagt tccacccaat acttccaaac
     2701 ctccggacag tgtattggtg gcagcatcat tggtgttcag atcttcaaat ggtcctgttg
     2761 acttgaatga ttttaaccaa tggtggagtt gggtaaatgg tgcaagttgg aagcatccac
     2821 aaggttctgg cagtactatt aatggaaaag aaaattatcc tgtagtgcaa gtaagttggg
     2881 atgatgcgat ggcttattgc aaatgggcag gtaaacgatt accaacggaa gcggaatggg
     2941 aatttgcagc aagaggcgga ttgat
//
LOCUS       DAOOOL010000229         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4069_length_2957_cov_50.307030, whole genome shotgun sequence.
ACCESSION   DAOOOL010000229 DAOOOL010000000
VERSION     DAOOOL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_2957_cov_50.307030"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1553
                     /gene="pbpC"
                     /locus_tag="PKU82_11960"
     CDS             <1..>1553
                     /gene="pbpC"
                     /locus_tag="PKU82_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOZ83621.1"
                     /translation="QLVKAIIQKEDRWFYFHYGVNPVAVGRAVVNNLIYQRKTSGAST
                     ITMQVSRLLSPAPRTLFNKMFEAFRAVQLENRYSKKEILEMYLNLIPYGGNIEGVKAA
                     SVLYLDKLPADLSLAECVTLCIIPNRPNSLRPGYDNDLIRRERNKWLTRFEKNKVFKK
                     EIINDALNEPVEMKRQAAPALITQLAYRLKEQYPTIENIHSTLQAPLQHKVQSLVYNY
                     SKRKSLQSIYNASAIVIDNKNHKVVAYIGSADFKDQLHHGQVDGVNAVRSPGSSLKPL
                     IYAMAFDKGLATPMTVINDVPVNYMGYNPENYNSKFNGRVTVDFALSHSLNIPAVKIL
                     NQLGVPTVTQALIKSGFSSIEKSKNKLGLSLALGGCGVRLFELTNMYAAFANNGVYQK
                     LKYVEADSLNSDTIRIISPGAAYLTTEILSKITRPDLPDNYANLLRIPHIAWKTGTSY
                     GRRDAWSIGYNKRFTVGVWLGNYNGSGVAELSGASVATPLLFDIFNAIDYDSDSTWFS
                     PPADVNFRL"
     assembly_gap    1554..1653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1654..1914
                     /locus_tag="PKU82_11965"
     CDS             <1654..1914
                     /locus_tag="PKU82_11965"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOZ83622.1"
                     /translation="GQPPKIVSPNMQSEYLLDLKGGDKIMLQCQASADVQNVYWYIND
                     LFYRAASANEQLFCSPPKGRVKISCADDMGRNSDIFIKIEQM"
     gene            complement(1916..2659)
                     /locus_tag="PKU82_11970"
     CDS             complement(1916..2659)
                     /locus_tag="PKU82_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOZ83623.1"
                     /translation="MNKILFLIPAYSFKKLLKHIDLKLLRSYWIGFALSLTTLTSVAQ
                     NKVFTGGASYYAKSFEGRKTANGELYSNYDMTCASRTLRFHTFLKVTNLKNKLVTIVR
                     VNDRGPYAKNRIIDLTEQAARIIGSYKHGITKVKLEIVQPPENTDSLEKYFMQEQVID
                     AEGKVVNPTGYTISIWRTRDFDHALLLTKYLQQEEYIQSFYVGKKYQNGRPLYHILVL
                     NISTQEEAVKLKDFWERKGFMRVRMLEKF"
     gene            2739..>2957
                     /locus_tag="PKU82_11975"
     CDS             2739..>2957
                     /locus_tag="PKU82_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83624.1"
                     /translation="MSTVKFNNKDAVFFPELKQRINQYFEQNKIKPTGNLKLYSKTII
                     LFTILIALYIILIEVRGNTVISCCSTTFT"
BASE COUNT          915 a          509 c          527 g          906 t
ORIGIN      
        1 acaattggta aaagctatta ttcaaaagga agaccgttgg ttttattttc attatggcgt
       61 aaatcccgta gccgttggca gggcagtagt gaataattta atttatcagc gaaaaacttc
      121 cggtgcatca accatcacca tgcaggtgtc aagattgctt agtcctgcac cacgcacctt
      181 gtttaacaaa atgtttgaag cattccgtgc agtacaattg gaaaacaggt attcaaagaa
      241 agaaatttta gagatgtatc tcaatctgat tccctatggt ggaaatattg aaggggtaaa
      301 agctgcatca gttttgtatc tcgacaaatt gcctgcagac ttatctcttg cagagtgtgt
      361 aacattgtgt attattccta acagaccaaa ctctctgcgc ccgggttatg ataatgatct
      421 catcaggcgc gaaagaaaca aatggctgac aagatttgaa aagaataaag tttttaaaaa
      481 agaaatcatc aacgatgcat taaatgaacc tgttgaaatg aagcgacagg ctgcacctgc
      541 attaattaca caattagcat ataggctaaa ggaacagtat ccaaccattg agaatattca
      601 ttctacttta caagcaccat tacagcataa agtacaatca ttagtgtata actacagtaa
      661 gcgtaagtca ctgcaaagta tttacaatgc atcagcaatt gtgattgaca ataaaaatca
      721 taaagtagta gcctatattg gctctgcaga ttttaaagat cagttacatc atggacaggt
      781 tgatggtgta aatgctgttc gttctcccgg cagtagcctc aaaccattaa tctatgcaat
      841 ggcttttgac aaaggtttgg caacacctat gacagtaatt aatgatgtgc ctgtaaatta
      901 tatgggatat aatcctgaaa actacaattc aaaatttaat ggcagagtta ctgtagattt
      961 tgctttatca cattcactga atattcctgc agtaaaaatt ctgaatcaat tgggggtgcc
     1021 tactgtaaca caggcattaa ttaaaagcgg ttttagctca attgaaaaaa gtaaaaacaa
     1081 actgggtttg tctttggcac ttggaggttg tggtgtgcgt ctttttgaac tcacaaacat
     1141 gtatgctgcc tttgcaaata atggagtcta tcagaaatta aaatatgttg aagcggattc
     1201 attgaatagt gacaccataa gaataatatc tcccggtgca gcctatttga ccactgaaat
     1261 actcagtaaa attacacgac ctgatttacc tgataattat gccaacctac ttcgtattcc
     1321 tcatattgct tggaaaaccg gaacttcgta tggtaggagg gatgcatgga gtattggcta
     1381 caacaagagg tttacagtag gggtgtggct tggaaattat aacggtagtg gcgttgccga
     1441 acttagcggt gcatcagttg caactccatt gctctttgat atatttaatg caatagatta
     1501 tgatagtgat tcaacatggt tcagcccgcc tgctgatgta aatttcagat tggnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggcagc caccaaaaat tgtttctccc
     1681 aatatgcaaa gtgaatattt gcttgattta aaaggaggag ataaaattat gcttcaatgt
     1741 caggcatctg ctgatgtgca gaatgtttac tggtacatta atgatttgtt ttatcgtgct
     1801 gcttcagcta atgagcaatt gttttgttct ccaccaaagg gtcgtgttaa aatttcttgt
     1861 gctgatgata tgggtcgcaa cagtgacatc tttattaaaa tagaacagat gtaattcaga
     1921 atttttccag catccttaca cgcataaaac cttttcgctc ccaaaaatcc ttcaacttaa
     1981 cagcttcttc ctgtgtagaa atgtttaaga ctaaaatatg gtaaagtggt ctgccattct
     2041 gatatttttt gccgacataa aaagattgaa tgtattcttc ctgttgcaaa tattttgtca
     2101 gtaaaagcgc atgatcaaaa tcgcgtgtgc gccaaatact gatggtgtaa ccggttggat
     2161 taacaacctt accttcagca tcaataactt gttcctgcat aaaatatttt tcaagagagt
     2221 cagtattttc aggcggttga actatttcaa gcttcacctt tgttatgcca tgtttatagc
     2281 ttccgataat acgtgcagcc tgttcagtaa ggtcaatgat gcggtttttt gcatagggac
     2341 ctctgtcatt aacacgtact atagttacca atttattttt caggtttgta acctttaaaa
     2401 aagtatgaaa ccttaatgtc ctgctggcac aggtcatgtc atagttacta tataattcac
     2461 cattagcagt ttttcgacct tcaaaactct tggcataata tgatgcacca ccggtaaata
     2521 ctttgttttg tgcaacggac gttaaagtcg ttaaactcaa agcaaaacct atccaatagc
     2581 ttctaagcaa ttttaaatca atatgtttga gtagtttctt gaatgagtaa gccggtataa
     2641 gaaataatat tttgttcatc tttttaagtg tatttctttt ttaagctaag taaagcacac
     2701 gctcaaaatg cctacatttg cagtcaatat ttaatatcat gtcaacggta aaattcaaca
     2761 ataaagatgc agtatttttt cctgaattaa agcaacgcat caatcaatat tttgaacaaa
     2821 ataaaataaa gcctaccggt aatctgaaat tgtattctaa aaccataata cttttcacca
     2881 ttttaatagc gctttatatt atactgattg aagttagagg caatactgtt atatcctgtt
     2941 gcagcacgac ctttacc
//
LOCUS       DAOOOL010000230         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4076_length_2951_cov_84.113605, whole genome shotgun sequence.
ACCESSION   DAOOOL010000230 DAOOOL010000000
VERSION     DAOOOL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4076_length_2951_cov_84.113605"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="rfbB"
                     /locus_tag="PKU82_11980"
     CDS             <1..1039
                     /gene="rfbB"
                     /locus_tag="PKU82_11980"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOZ83625.1"
                     /translation="GAGFIGSHVVRRFVNNYSDYTIYNLDNLTYAGNLANLKDIESKS
                     NYHFIKGDITDADFVNKLFADINPDAVIHLAAESHVDRSITNPLDFIMTNIVGTVNLL
                     NAARNTWIKDKKVSPGKRFYHISTDEVYGSLGETGFFTENTSYDPRSPYSASKASSDH
                     LVMAYHHTYHLPVVLSNCSNNYGSHHFPEKLIPLAINNIKNMKPVPVYGKGENVRDWL
                     YVEDHARAIDVIFHKGKEGEKYNVGGNNEWKNIDLIQLLCSVMDKKLNRKEGTSASLI
                     TFVTDRPGHDLRYAIDSTKLQNELGWKPSLQFEEGLEKTVDWYLANDEWLQQVTSGEY
                     MTYYQHQYDAR"
     gene            1049..2050
                     /locus_tag="PKU82_11985"
     CDS             1049..2050
                     /locus_tag="PKU82_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ83626.1"
                     /translation="MKETIYERAYHQQSLKDKAFLITGGSGFIGSNIVEYLLVHGAGK
                     VRVLDNLANGYKKNIEPFLNHPSFEFFEGDITNADTCLKAMEGIQFVSHQAALGSVPR
                     SLANPLATHHANATGFLNMLWAARQMNIERIVYASSSSVYGDSPELPKREHITGNPLS
                     PYAVSKMSNELYAYTFCMHYDMQIIGLRYFNVFGPNQNPDNPYAAAIPLFMKALLKDQ
                     PAYIFGDGEQSRDFTFVENAVQANIRALFSEHKDACNKVYNIAVGESISLNKLLHHLM
                     HITGKKIEPVYKEERPGDVKNSLADISLAKQYLGYNPLVKVKEGLALTVPWFKKNFC"
     gene            2112..2762
                     /locus_tag="PKU82_11990"
     CDS             2112..2762
                     /locus_tag="PKU82_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HOZ83627.1"
                     /translation="MKKLFVLALLVVSSFSINAQKLKEGTAHYKIDYNIESDEGGMKG
                     MLPTDANLSFKGDMFMMETTGGMFDQKTISDSKKQESTMLMDMMGNKVAVKVTKADID
                     KEKNKGTKPKVEITNETKTIAGYNCTKAIVKMEGAEPAEIWFTKDLAINNGFSQGYDG
                     IDGMMLEYTIPQKMFTMKMTCTEIKADKVDDKVFEVPSDYKPMTKEELMKMSGGGH"
BASE COUNT          987 a          534 c          574 g          856 t
ORIGIN      
        1 cggtgccgga ttcataggtt cgcatgttgt gcgcagattt gtaaacaatt attccgatta
       61 cacaatttac aacctcgaca acttaaccta tgcaggaaat cttgctaacc tgaaagatat
      121 agaatcaaaa tcaaactatc attttataaa aggtgatatt accgatgctg actttgtaaa
      181 caaactgttt gcagatataa atcccgatgc agtaattcat cttgctgcag aatcgcatgt
      241 ggacagaagt attaccaacc cactcgattt tatcatgacc aatattgtgg gtacggttaa
      301 ccttcttaat gctgcacgaa atacgtggat aaaagacaaa aaagtttctc ccggaaaaag
      361 attttatcat atcagtactg atgaagtgta tggctctctt ggcgaaacag gtttctttac
      421 tgaaaacacc tcttacgatc cgcgcagccc ttattctgca tctaaagcaa gctccgatca
      481 tttggtaatg gcctatcatc acacctatca tttgcccgta gtactttcta actgctccaa
      541 caactatggc tcacatcatt ttcctgaaaa gctgataccg ttagccatca acaatattaa
      601 aaatatgaag cctgtgcctg tttatggtaa aggcgaaaat gtgcgcgact ggttatatgt
      661 tgaagatcat gcacgtgcta ttgatgtaat ttttcacaaa ggaaaagaag gtgagaaata
      721 taatgttggc ggcaacaatg aatggaaaaa tattgatttg atacaattgc tttgttctgt
      781 gatggataaa aaattaaatc gaaaagaagg tacatcagca tcactcataa catttgtgac
      841 agacagaccc ggacatgatt tacgctatgc tatagattct acaaaacttc agaatgaatt
      901 aggatggaaa ccatcactgc aatttgaaga aggacttgaa aaaactgttg actggtatct
      961 tgccaatgat gagtggctgc aacaggtaac ttccggtgaa tatatgacct attaccagca
     1021 tcagtacgat gcacgataac cccaaaagat gaaagaaact atctatgaac gcgcctacca
     1081 tcagcagtct ttaaaagaca aagcattcct tataaccgga ggctccggtt ttattggaag
     1141 caacattgtt gagtacttgt tggtgcatgg tgcaggaaaa gttcgtgtgc tcgataatct
     1201 tgcaaacggc tacaaaaaaa atattgaacc ttttctgaat catccatcat ttgaattttt
     1261 tgaaggcgac attaccaatg ccgatacatg cctaaaagca atggaaggaa ttcagtttgt
     1321 atcgcatcag gctgcattag gttctgtgcc gcgttcgttg gccaatcctt tagcaacaca
     1381 tcatgccaat gcaacaggat ttttaaatat gttgtgggca gcacgtcaga tgaatattga
     1441 gcgtattgtt tatgccagtt catcatctgt ttatggcgac agccccgaac ttcccaagcg
     1501 cgaacacata acaggcaatc cattatcgcc ttatgctgtt tccaaaatgt cgaacgaact
     1561 ttatgcttac acattctgca tgcattacga tatgcagatt ataggtctgc gttattttaa
     1621 tgttttcggt cccaatcaaa atcccgacaa tccttatgca gcggctatac ctctttttat
     1681 gaaagcgctg ctcaaagatc agcctgccta tatttttggt gatggcgaac aatcgcgcga
     1741 ttttactttt gttgaaaatg ctgtgcaggc aaacatcaga gccttattca gcgaacataa
     1801 agatgcctgc aataaagttt ataacattgc tgtgggcgaa agtatcagtc ttaataaatt
     1861 gctacatcat ctgatgcaca ttacaggtaa aaaaatagaa cctgtttata aagaagaacg
     1921 cccgggtgat gtaaaaaatt ctttggcaga tattagtctg gcaaagcaat atctcggcta
     1981 caatcccttg gtaaaagtca aggaaggact tgcccttacg gtgccctggt ttaagaaaaa
     2041 tttctgctaa acagtattcc atcttattgt ctttactact ttcgcttaat aacgatttaa
     2101 acaaaaatat tatgaaaaaa ttatttgttc ttgcattact tgttgtgagc agcttcagca
     2161 tcaatgcaca aaaactaaaa gaaggtactg cacattataa aatagattac aacatagaat
     2221 ctgacgaagg aggcatgaaa ggcatgctgc ctacagatgc caacttatcg tttaagggcg
     2281 atatgtttat gatggaaaca acaggtggca tgtttgatca gaaaacaatt tccgactcta
     2341 aaaaacaaga gtcaaccatg cttatggata tgatgggaaa taaagtggca gttaaagtta
     2401 ctaaggctga tattgataaa gagaaaaaca aaggaaccaa acctaaagtt gaaatcacta
     2461 acgaaacaaa aactattgct ggctacaact gtaccaaagc tatagtaaaa atggaaggtg
     2521 cagaacctgc tgaaatctgg tttacaaaag acttggccat caacaacggt tttagtcaag
     2581 gttatgatgg tattgacggt atgatgttgg aatatactat accacaaaaa atgtttacta
     2641 tgaaaatgac ttgcacagag attaaagccg ataaagttga tgacaaagtt tttgaggtgc
     2701 catcagacta taagccaatg acaaaagaag aacttatgaa aatgagtggt ggtgggcatt
     2761 aagtttgaag gtttagaaag ttgaagtaat tccgataaac tttaattttt aggttaaata
     2821 taaaaccact gttatgttcc tgattttaat cgggtaatca tgatggtggt tttttattgg
     2881 agatatatta aattttagat ttgaatatta ttgtcagaag tccttcaatt ctatattggt
     2941 ggaaacagtg a
//
LOCUS       DAOOOL010000231         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4175_length_2905_cov_87.216842, whole genome shotgun sequence.
ACCESSION   DAOOOL010000231 DAOOOL010000000
VERSION     DAOOOL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4175_length_2905_cov_87.216842"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PKU82_11995"
     CDS             <1..1473
                     /locus_tag="PKU82_11995"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83628.1"
                     /translation="IWDGPFGTFFNGNPMNQSPYTTTTPSVQITFSSSTTSFVNVGVQ
                     AANGCGASLRKIQKVRYQISTPKEITGATTMCASTNGTYTIAPVTDATSYQWMITGNA
                     TVVPSGTSAVVFFGPSWNGGTLCVAAKTPCYTTAYKCIAISKSASALNAITGPLSSCP
                     NEVQTFNVLPCTGAASYNWTLPAGATGSSSTNSITATFGPTFTVGGNICVSVTSICGV
                     TSTPKCRTVAQGLPSVPTSISGITNGLCNQSVNYTVPSSPGVTYNWTAPGTVIGNGNS
                     SVSVTYGALTTGQLCVTASNSCGTSAARCTPLKGAPNSPIALTAIPASWCANTQGIEF
                     TADLGNTTGSYTLSWGYPSSPTASYVAGGGNSNALTLDWGTGNGSVVVTATNSCGAGS
                     KAFAVTIGCKEGEMAKANQLNVYPNPTAGVLNVEYTAEKGTAQVTVLDLSGRVVMTQT
                     HANAAGQNTLQLDLSKVAKGAYMLNVQTNGSNNQVRVVVE"
     gene            1542..2066
                     /locus_tag="PKU82_12000"
     CDS             1542..2066
                     /locus_tag="PKU82_12000"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ83629.1"
                     /translation="MMNDRIKILKANLNDTTALSVIAATTFYDTYGAYNKQETIQRYI
                     DKWFSTSQMCKELSDDRIIYYLVYDQQQLSGYIKLQFVNPHQNSDLNGVELSRLYLLK
                     QWHGSGVSEVMLNHCYKIAEELKQDAIWLSVWEKNNRAIRFYEKHGFVKKGNLIFKMD
                     DEDQNDFLMVKHLI"
     gene            complement(2191..>2905)
                     /locus_tag="PKU82_12005"
     CDS             complement(2191..>2905)
                     /locus_tag="PKU82_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOZ83630.1"
                     /translation="RIENIVHKIQFESVKSELKNWKEHNQKNLLAGLITVARYQYPDL
                     DEDAIRAFFSEVRRRVWDDLQPNDTPLEQVRILNQVLFVEFGFNGNTANFHSPQNSFI
                     NTVIESKRGNPILLSVIYSIVAQDLDIPIYGVNLPEHFVLCYQTLNKDKFAEYAFPEE
                     NILFFINAFSRGSVFGKNEIDLYLKKLRLQPDKKFITPCSNTDIVRRILHNLNFSFNK
                     SGLTEKSNEIETLLSLLEI"
BASE COUNT          943 a          535 c          623 g          804 t
ORIGIN      
        1 atctgggatg gcccattcgg taccttcttt aacggtaatc cgatgaatca atcgccttat
       61 acaacaacaa caccaagtgt gcagatcacg ttcagctcgt caaccacatc gtttgtaaat
      121 gtaggcgttc aggcagccaa cggttgcgga gcatccctac gtaagatcca gaaggttaga
      181 tatcagataa gcaccccaaa ggagattacc ggagcaacaa caatgtgtgc cagcactaat
      241 ggaacatata cgatagcccc tgtaacagat gccacaagtt atcagtggat gataacaggc
      301 aatgccacag tagtgcccag tggcacatca gcagtagtgt tctttggccc aagttggaat
      361 ggcggcacat tatgcgtagc agccaagaca ccatgctata ctacagcgta taagtgcata
      421 gccataagca aatcggcttc agcattgaat gctatcactg gcccattgtc atcatgtcca
      481 aacgaggtac agacctttaa tgtactacca tgtacaggag cagcgagcta caactggacg
      541 ttgccggcag gagctacagg cagttcatca acgaacagca tcacagccac gtttggccca
      601 acgtttacag taggaggcaa catttgtgta agtgttacaa gtatctgtgg agtaacctca
      661 acacctaagt gcagaacggt agctcaaggc ttgccatcag taccgacaag catcagtggc
      721 atcaccaatg gattgtgtaa tcagagtgtg aactacacag taccatcatc tccaggcgtt
      781 acgtataatt ggacagcacc cggcacagta ataggtaatg gcaacagttc cgtaagtgtt
      841 acttatggag cattaacaac aggtcagttg tgtgtaacag cgagcaacag ttgtggcaca
      901 agtgcagcaa gatgtacacc actgaagggg gcaccaaact caccgattgc attaacagcc
      961 atacctgcaa gctggtgtgc caatacacag ggcatagagt ttacagcaga cttaggtaac
     1021 acaacaggtt cttatacatt atcatggggc tatccatcat caccaacggc aagttatgta
     1081 gcaggtggag gaaactccaa tgcactaacg ttggattggg gcacaggcaa tggctcggta
     1141 gtagtaacag cgacaaatag ttgcggagca ggatcaaaag cctttgcagt gactatagga
     1201 tgtaaggaag gtgagatggc aaaagccaat cagttaaatg tatatccaaa tccaacagca
     1261 ggtgtgttga atgtagaata cacagcagag aaaggtacag cacaggtaac ggtgcttgac
     1321 ctgagcggcc gtgtagtaat gacacagaca catgccaatg cagcaggaca aaacacatta
     1381 caactagacc tgagcaaggt agctaaagga gcgtacatgt tgaatgtaca aaccaatggc
     1441 agcaacaatc aggtgagagt ggtagtggag tagtttttgg ttaaataaat tgctaagggg
     1501 cagtgcgaaa gcactgcccc ttatttttgc cttatacttt aatgatgaat gatagaataa
     1561 aaatattaaa ggcaaatttg aatgatacaa cagcattatc agtgatagct gcaactactt
     1621 tttatgatac ttatggtgct tacaataagc aggaaacgat acaacgttat atagataaat
     1681 ggttcagcac ttcgcaaatg tgtaaggagt tatcagatga tagaataatt tattatcttg
     1741 tttacgatca acaacaactt tccggctata taaaattaca atttgtaaac ccacatcaaa
     1801 atagtgattt aaatggcgtg gagttgagca gactttattt gttgaaacaa tggcatggca
     1861 gtggcgtttc cgaagtcatg ttgaatcatt gctataagat tgctgaagag ttgaagcagg
     1921 atgcaatttg gttgtctgtg tgggaaaaga ataatagagc aatacgtttt tatgaaaaac
     1981 atggatttgt taagaaaggc aatctgatat ttaagatgga tgatgaagac caaaatgatt
     2041 ttttaatggt aaaacatctg atttaaaatg taatattcct tcgtaataaa tagtttctgc
     2101 gcaaactgaa gatatatatt gtaattgaat aggtttattg taaatacata ttatttgaat
     2161 taaccgcttc ctactggcag gaagcggttt ttatatttct aataaagaga gaagagtttc
     2221 tatttcattt gatttctctg tcagacccga tttgttgaat gaaaaattta aattgtgtaa
     2281 tattcgtctg acgatatctg tattactgca aggtgtaata aattttttat caggctgaag
     2341 tcttaatttt ttcaggtata gatcaatttc atttttaccg aatacagagc ctctgctgaa
     2401 cgcattgata aaaaacaaaa tattttcttc cggaaaagca tactcagcaa atttatcttt
     2461 atttaatgtt tgataacaca atacaaaatg ctccggtagg tttacaccat aaatgggaat
     2521 gtcaagatcc tgagcaacga tagaataaat tacgctcagt aaaataggat tgcctctttt
     2581 gctttcaata actgtattga taaatgagtt ctgaggtgag tgaaaattgg ctgtgttgcc
     2641 attaaaacca aattctacga atagaacctg attcagaatt ctgacttgtt ctaaaggcgt
     2701 gtcgttaggt tgaagatcat cccaaacacg tcttcttact tcgctaaaaa atgctctgat
     2761 ggcgtcctcg tctaagtcgg gatattgata acgtgcaaca gttattaaac cggcaagtaa
     2821 gtttttttgg ttatgttctt tccagttttt taattctgat tttacagatt caaactgaat
     2881 tttatgtaca atgttttcaa ttcgc
//
LOCUS       DAOOOL010000232         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4256_length_2864_cov_39.084372, whole genome shotgun sequence.
ACCESSION   DAOOOL010000232 DAOOOL010000000
VERSION     DAOOOL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4256_length_2864_cov_39.084372"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKU82_12010"
     CDS             <1..319
                     /locus_tag="PKU82_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83631.1"
                     /translation="YKGLYSWIEDISKLSRDAQKNFLNFSLGTVRECLITNMSGINHS
                     RYTDATFNGFEKFSKMINAENIEPFAKTLEEAIYHIERNANPKILFLDLSFRMHRILH
                     MKF"
     gene            327..1514
                     /gene="ricT"
                     /locus_tag="PKU82_12015"
     CDS             327..1514
                     /gene="ricT"
                     /locus_tag="PKU82_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOZ83632.1"
                     /translation="MNTETINTNTKSTGCTSCGTGSCNKLNVYDWLSDMEFPPGHIPF
                     NVVEVQFKGTRKEYFRNTDGHILKAGDIVAVEGNPGHDIGTVTMTGELVKLQLKKRGF
                     ESGAPEIKQLYRLAKQGDIEKWEQVKQLEHQTMHRARKIALQLGLKMKLSDVEYQGDG
                     KKATFFYTAEERVDFRELIKKLHEEFKVRIDMRQIGLRQEAGRLGGIGSCGRELCCST
                     WMTSFKTVGTSAARYQNLSINPVKLAGQCGKLKCCLNFELDSYMDALKDIPEQGTAIF
                     TEAGKALHRKTDIFKRIMWYELIPNLKDGEYYQPSADKWVMIPVDRVKEIIALNKENQ
                     KPADLKSGLPVEEEVEEPDYADMVGQDSLTRMDKKRKKKKKKKKPSQPNQQTNNGQQK
                     KPS"
     gene            1489..1971
                     /locus_tag="PKU82_12020"
     CDS             1489..1971
                     /locus_tag="PKU82_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOZ83633.1"
                     /translation="MANRKSLLSYLIASVLILVSASCNKQYHYEENKSIKGSNWDFSE
                     PISFDVNIDDTVTAYNMYINVRNQGNYRFSNLFLFVNTHLPQGQHLRDTVEITLATPD
                     GAWLGKGIGDLYSNRYLFRKNFRFPQKGNYHFELIQAMRVNPLPGIMDAGIRIENTKP
                     "
     gene            complement(2058..>2864)
                     /locus_tag="PKU82_12025"
     CDS             complement(2058..>2864)
                     /locus_tag="PKU82_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83634.1"
                     /translation="AGLLLTGSCSNKSGEENKEQTTETKDTMPVSEVATFQFTYTIAN
                     LPPPMQVLDEFSKSKLEVNTALLNSADNADKYMTSLKQAMNYGIYGVDLGYLVVNNRT
                     LDAIKYYNTAKNLAAQLGMEETFNQFVNRFETNSNNRDSLTRVIDDAYSATDAYLRSN
                     KRLETASQVLAGSWLECQYLTVKSLLTATRNADNEILYKRVWEQRLYLDNITKLLSEF
                     ADSDDSKKIKSDYEALLAIYKEPADEKQIDNAFLTRLASGLEKARQNIVE"
BASE COUNT          940 a          499 c          571 g          854 t
ORIGIN      
        1 ttataaagga ctatacagtt ggattgaaga catatcaaaa ctatcgcgcg atgcacaaaa
       61 gaacttttta aattttagtc tgggcactgt ccgcgaatgt ttaattacca acatgtcagg
      121 tataaatcat tcaagatata cagatgcaac gtttaatggt ttcgaaaaat tcagcaagat
      181 gataaatgct gaaaacattg agccttttgc aaaaacactt gaagaagcta tttatcatat
      241 tgagcgcaat gccaatccaa aaatattatt tttagatttg tcgttcagaa tgcatcgcat
      301 tttgcacatg aaattttgat tgattaatga ataccgaaac aataaataca aatacaaaaa
      361 gtacaggttg cacttcatgc ggaacaggca gttgcaataa actgaatgtt tacgactggc
      421 ttagtgatat ggagtttcca ccgggacata ttccattcaa cgtagtagaa gtgcagttta
      481 aaggaacccg aaaagaatat ttcagaaata cagacggaca catacttaaa gccggagata
      541 tagttgccgt tgaaggaaat cccggacatg atattggcac cgtcaccatg acaggtgagt
      601 tggttaaact tcaacttaaa aaacgtggtt tcgaaagtgg tgctccggag attaagcaac
      661 tgtacagact ggctaaacaa ggcgatattg aaaagtggga gcaggtaaaa caattagaac
      721 atcaaaccat gcaccgtgca cgtaagattg cattgcagct gggtctgaag atgaaactta
      781 gtgatgtaga atatcagggt gatggtaaga aggctacctt tttttatact gcagaagaac
      841 gtgttgattt tcgtgagttg attaaaaaac tacatgaaga atttaaggtg agaattgata
      901 tgaggcagat tggtctcaga caagaagcag gacgtcttgg tggaataggt tcgtgtggaa
      961 gagagctttg ctgttctaca tggatgacaa gttttaaaac tgttggtaca tcagcggcac
     1021 gatatcagaa tctatccatc aatcctgtaa aacttgccgg acagtgtggt aaactaaaat
     1081 gctgtctcaa ctttgagttg gacagttata tggatgccct taaagatatt cctgaacaag
     1141 gtactgctat ttttactgaa gcaggaaaag cattacacag aaagacagat atttttaaac
     1201 gcattatgtg gtatgaacta attcctaatt taaaagatgg cgaatactac caacccagtg
     1261 cagataaatg ggtaatgatt cctgttgaca gagtaaaaga aattattgca ctcaataaag
     1321 aaaaccaaaa gccggctgat ctgaagagtg gtcttcctgt agaagaagag gtagaagagc
     1381 cggattatgc cgacatggtt ggacaagata gtcttaccag aatggataag aagagaaaga
     1441 agaaaaagaa gaagaaaaaa ccttcacagc ctaatcaaca aactaacaat ggccaacaga
     1501 aaaagccttc ttagctattt aattgcaagc gttttaatct tggtatctgc ttcctgtaat
     1561 aagcaatatc attatgaaga aaacaaatcc ataaaaggaa gcaattggga tttttctgaa
     1621 ccaataagtt ttgatgtcaa cattgatgat accgtcactg cttataacat gtacattaat
     1681 gtgcgtaatc agggtaacta tcgtttcagt aatttgtttt tgtttgttaa cacacattta
     1741 ccacaaggac agcatttacg cgatacagtt gaaataactt tagctactcc tgacggggcc
     1801 tggcttggca aaggcattgg tgatttatat tccaatcgtt atttgtttcg caaaaatttc
     1861 agattccctc aaaaaggtaa ctatcatttt gaactgatac aggcaatgcg tgtaaatcca
     1921 cttccgggaa ttatggatgc tggtatcaga atagaaaata caaaaccata atttttagcg
     1981 aattttcttt accgatagaa aaaataaaaa gccgcctcat taactgaaac ggctgcttgg
     2041 ttttagactt aattatttta ttctacaata ttctgacgtg ctttttccaa acctgatgcc
     2101 aatcgggtta agaacgcatt gtctatttgt ttttcgtctg caggttcttt ataaatagct
     2161 agtaaggctt catagtctga ttttattttc tttgaatcat cactatcggc aaactcactt
     2221 aacaatttcg taatgttgtc aagatacaaa cgttgctccc atactctttt atacaatatt
     2281 tcattgtcgg catttctggt tgctgtaagc aatgatttta cggtaagata ttgacattcg
     2341 agccagcttc cggcaagtac ctgactggca gtctccaacc ttttgttgct gcgtaaataa
     2401 gcatcagttg cagagtatgc atcatcaata acacgagtca gagaatcacg attgttgctg
     2461 tttgtttcaa aacgatttac aaactgatta aatgtttcct ccatgcctaa ttgtgcagca
     2521 agattctttg ctgtgttgta atacttaatg gcatcaaggg ttctgttgtt aacaaccaaa
     2581 taacccaaat caacaccata aatcccgtag ttcatggctt gtttaagaga tgtcatgtat
     2641 ttgtcggcat tatctgctga attgagtaat gcagtgttta cttcaagttt tgatttacta
     2701 aactcatcta atacctgcat aggtggaggc aggttggcaa tggtgtaggt aaactgaaaa
     2761 gttgctacct cactaaccgg catggtatct tttgtttctg tagtctgctc tttgttttct
     2821 tcaccagact tattactaca tgaaccggtg agcaacaatc cggc
//
LOCUS       DAOOOL010000233         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4258_length_2863_cov_100.863960, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000233 DAOOOL010000000
VERSION     DAOOOL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_2863_cov_100.863960"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKU82_12030"
     CDS             <1..260
                     /locus_tag="PKU82_12030"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HOZ83635.1"
                     /translation="ISFNLSYLLSAAAIILLISLYAKTIFNNLRLALLFSLVLIILYV
                     FVFSIIQLQDYALLLGSIGLFIVLALVMYLSRKINWYGSEK"
     gene            244..621
                     /locus_tag="PKU82_12035"
     CDS             244..621
                     /locus_tag="PKU82_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOZ83636.1"
                     /translation="MVQKNKGDHYQLGREGENMAVEYLIANGYKIVERNWRFGKLEID
                     IIASKGNLLCIVEVKTRTGDYFGEPEEGVTKAKERFLATAADHYIISKNLDVEVRYDI
                     VSIVIYNGRHELKFIEDAFYPYS"
     gene            complement(623..1249)
                     /locus_tag="PKU82_12040"
     CDS             complement(623..1249)
                     /locus_tag="PKU82_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOZ83637.1"
                     /translation="MENITESLEFKSSPALREKLISYGTTKKFNAGDVILNENAYIRS
                     IPIVLSGSIRVMRNDDEGKEILLYYIKSGESCIMSFLGGIHQDTSKVRAVAEDETEIL
                     FVPVDKVITLIREHDDWLDFIFRLYHKRFEELLEVVNAVAFKKIDERLILLLQKKVEL
                     SKSKTITVTHEQLANELGTARVVVSRLLKQLEVDGRVELGRNKITLLQ"
     gene            complement(1282..1614)
                     /locus_tag="PKU82_12045"
     CDS             complement(1282..1614)
                     /locus_tag="PKU82_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83638.1"
                     /translation="MTSSQPLYTDNDFLRLITHCTSADEVKKVLSSHNIADADTKSSE
                     LFKKLCCIKRQTKGIYLMAAGAFMGFIGCLLSIVNPIPFLFDFALYGLTPGAVLVVLI
                     GLYLFIEG"
     gene            1746..2687
                     /locus_tag="PKU82_12050"
     CDS             1746..2687
                     /locus_tag="PKU82_12050"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOZ83639.1"
                     /translation="MKRTITPLVALCMMAATSFAQTTIVLQPGPADGKDAEIFSCVSC
                     GYAGNNYGTKRDLNAIAWTKSGNNSNVRSLIQFDLSAIPSNAFINSATLSLYFNPTSE
                     EGKHYCYPHSNSSYLERITSNWSETTVTWNNQPSTTTQNRVSLGGTTSSTQSFTNINV
                     RQLIIDSRNNPSTSFGFMLRLQQEHKFKKLIFASSDNTTAAIRPKLTITYTVPAVVSS
                     GHSMARLDEGQTFASISVFPNPVSGIATIQLPDDAGNGAVKIETFNMLGKLCNRIETY
                     VNADGVINFDASALPQGVYVVKVFCNEKVYTQRIVIK"
BASE COUNT          912 a          534 c          533 g          884 t
ORIGIN      
        1 acatcagttt caatttatca tatctgttat ctgctgctgc tattatttta ctcatcagcc
       61 tatatgcaaa aacaatattc aacaacctgc gcttagctct gttgtttagt ctggtgctga
      121 taattctgta tgtatttgtg ttttcaatca ttcagttaca ggactatgca ttgctgctcg
      181 gaagtatagg tttgtttatc gtgcttgcat tggtaatgta cttgtcgcgg aaaatcaact
      241 ggtatggttc agaaaaataa gggagatcat tatcagcttg gtcgcgaagg cgaaaacatg
      301 gcagttgaat atttaattgc caatggctat aagattgtag aacgcaactg gcgttttgga
      361 aaattggaga ttgatattat agcctctaaa ggcaacctgt tgtgcattgt cgaagtaaag
      421 actagaacgg gagattattt tggagagccc gaagaaggtg ttaccaaagc taaagaacgt
      481 tttctggcaa cggctgccga ccactacatc atttcaaaaa atttagatgt ggaggtgcgc
      541 tatgatattg tttccattgt aatttacaat ggccgtcatg aattgaaatt tattgaagat
      601 gctttttatc cttactcctg aattattgca gaagtgtaat tttatttcgt cccagttcca
      661 cgcgaccatc aacctctagt tgtttcaaca aacgcgaaac aactacacgt gcagtgccca
      721 actcattggc taattgttca tgagtaacgg taatggtttt tgacttagaa agttcaactt
      781 ttttctgaag taataaaatc aagcgttcat caattttttt gaaagctact gcattcacta
      841 cttcaagaag ctcttcaaag cgtttgtgat acagtctgaa aataaagtca agccagtcgt
      901 catgttcgcg aatgagtgtg attaccttgt caacgggaac aaataaaatt tctgtttcat
      961 cttcggcaac agctctcacc ttgcttgtgt cctgatgtat gccacccaga aaagacatga
     1021 tgcaactttc gccactttta atatagtaaa gtaaaatctc ttttccttcg tcatcattac
     1081 gcatcactct gatggagccg ctgagaacaa taggaataga acggatgtag gcattttcgt
     1141 ttaatattac atctccggca ttaaattttt tagttgttcc atacgaaatt agtttttcgc
     1201 gaagtgcggg tgacgattta aattcaagcg attcagtaat attttccata cagcaaaagt
     1261 aaacaatgtt gttgcaacaa ttcagccttc aataaacaag tataaaccaa tcagaacaac
     1321 caacactgcg cctggtgtga gtccatagag tgcaaaatcg aataaaaaag gtattggatt
     1381 aactatagaa agtaaacagc ctataaaacc cataaaggca cctgcagcca tcagataaat
     1441 acccttggtt tggcgtttta tacaacacaa ctttttaaac aactctgacg attttgtatc
     1501 ggcatcagca atattgtgtg atgaaagcac cttttttacc tcatcggcag aagtgcaatg
     1561 tgtaatgagt cggagaaaat cattatctgt gtaaagaggt tggctgcttg tcatatttgc
     1621 gttagtgttt aatgatgata aatgctatgg taagggaaaa tcaaaaataa ttaaaatatg
     1681 tcaaaaaaat gcagttggtt ttaaaaaaag tctttacttt gttatcaatt acaaaaacca
     1741 ttattatgaa aagaacaatt accccacttg ttgcgctttg catgatggca gcaacatcgt
     1801 ttgcacaaac aaccattgta ttacaacccg gacctgccga tggcaaggat gcagaaattt
     1861 tcagttgtgt aagctgcgga tatgccggca acaactatgg tacaaaacgc gacctgaatg
     1921 ctattgcctg gacaaagagc ggcaacaact ccaatgtgcg ttcgcttatt cagtttgatt
     1981 taagtgctat tccttccaat gcatttatca atagtgcaac actatctctt tattttaatc
     2041 caacctcaga agaaggcaaa cactattgtt atccacacag taattcaagt tatttggaac
     2101 gcatcacctc caattggagc gaaacaactg tgacatggaa caatcagcca tcaaccacaa
     2161 cacaaaacag ggtttcttta gggggcacca catcttcaac acaaagtttt acaaatatta
     2221 atgtaaggca gcttattatt gattcgagaa acaatccttc aactagcttt ggatttatgc
     2281 tcagacttca gcaagaacat aaatttaaaa aattgatttt tgcttcgagt gataacacta
     2341 cagcagccat cagacccaaa ctaactatta cctacacagt gcctgcagtg gtaagtagtg
     2401 gtcattcaat ggcgcgattg gatgaaggac aaacttttgc atcaataagt gtttttccaa
     2461 atcctgtttc aggaatagca actattcaac ttcctgacga tgccggaaac ggtgctgtta
     2521 aaattgaaac cttcaatatg cttggcaaac tttgtaacag aatagaaact tatgtgaatg
     2581 cagatggagt tataaatttt gatgcatcgg cactaccaca aggtgtttat gttgtgaaag
     2641 ttttttgcaa cgaaaaagtt tatacgcaac gcatagtaat aaaataattc tgtaattttt
     2701 tcaggtattt ctgtttctat tcatataaca gcggttttca ctaatgagag ttaagtgatt
     2761 tgccatgggt gaaaacataa ataaatcatt ttcggacacc ctaacatagc ctgttttttt
     2821 gtttctttgg caagagaaaa aaccaggcat ttctatctat gtc
//
LOCUS       DAOOOL010000234         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4285_length_2851_cov_44.391273, whole genome shotgun sequence.
ACCESSION   DAOOOL010000234 DAOOOL010000000
VERSION     DAOOOL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4285_length_2851_cov_44.391273"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKU82_12055"
     CDS             complement(<1..898)
                     /locus_tag="PKU82_12055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOZ83640.1"
                     /translation="MQRVVITGIGIYSCIGKNLAEVTESLRQGKSGIIFDQERKDLGF
                     RSALTGFVETPDLKDFLHRRDRLGMHQPAMYAFMSTREALAMAKLDTDYMEKNETGII
                     FGNDSTAGSVVETIDKARKKKDNTLIGSGDIFQNMNCTVNMNLATIYKLKGINFTLSA
                     ACASGSHAIGMGYLLIRQGLQERVICGGAQEINPPAMTSFDGLGTFSSRENEPQKASR
                     PFDKSRDGLIPSGGAATVILESLESAQKRGATILAEVTGYGFSSNGDHISNPDFNGQE
                     RSLNMAMKLAGINASNIDYVNAH"
     gene            complement(902..1633)
                     /gene="fabG"
                     /locus_tag="PKU82_12060"
     CDS             complement(902..1633)
                     /gene="fabG"
                     /locus_tag="PKU82_12060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOZ83641.1"
                     /translation="MNCALVTGASRGLGKAIALQLAADHKLHILINYASNKQAAEETL
                     TTIKNAGGSGELLPFNVQDNETVNAALDHWHKENENKFISVLVNNAGITRDGLFMWMP
                     EKDWDDVLNISLKGFYNVTRNVVNPMLRKRAGRIVNIVSLSGLKGNAGQTNYAAAKGA
                     IVAATKSLAFEVAKRKITVNAVAPGFIKSDMTEGLNEKELSKLVPMERFGTPEEVAHV
                     VSFLASDKASYITGEIININGGIYS"
     gene            complement(1637..>2851)
                     /locus_tag="PKU82_12065"
     CDS             complement(1637..>2851)
                     /locus_tag="PKU82_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOZ83642.1"
                     /translation="HSGVSPEVISLLTTMLNENIVPEIYEHGGVGASGDLVQLAHLGL
                     NLIGEGYVHYDGKRQKTADVFKKLNIKPLTLQLRDGLGIINGTSCMTGIAAINLVYAK
                     RLTRWMISLSAVINEIIESFDDSFSAELNHVKLHNGQREVAQQMREFLKGSKLVRSRK
                     ELFSDHAELSRTEFKNKIQEYYSIRCVPQIIGPIYDTCMAAIAVTENELNSTNDNPVV
                     MPEADNVFHGGNFHGDYISLEMDKLKLAISKLSVLAERQLNFLLNKKLNRKFPPFLNK
                     KKLGFNFGLQGMQFLATSTTAENQSLSNSVYVHSISNNNDNQDVVSMGTNSAIIARKV
                     IDNSYEVVAVLTLAVCQAIDLLSTEESQKLCKNTLSLHGLIRKQVAVVEDDVPQFENI
                     RKVIDILKENEL"
BASE COUNT          789 a          599 c          536 g          927 t
ORIGIN      
        1 catgggcatt tacgtagtca atatttgatg cattaattcc tgctaacttc attgccatgt
       61 ttaatgagcg ttcttgccca ttaaaatcag gatttgaaat atgatcgcca ttggatgaaa
      121 atccatagcc tgttacttca gcaagtatgg tagcacctct tttctgtgcc gattcaagcg
      181 actctaaaat tacagtggca gcaccacctg aaggaattag tccgtcacgg cttttatcaa
      241 atggacgcga tgctttttgt ggttcattct cacgactgct gaatgtgcca agcccatcga
      301 agctggtcat agcaggtgga tttatttctt gtgcaccacc gcagataaca cgctcctgta
      361 atccttgacg tatcaacagg taacccatac caatggcatg tgatccactt gcgcaagctg
      421 cactaagtgt aaaatttatt cctttaagct tataaatagt ggcaaggttc atgtttaccg
      481 tgcagttcat gttttgaaaa atatcgccac tgcctattaa agtattgtct ttcttttttc
      541 ttgccttgtc aatagtttct actacagaac ctgctgtgct gtcattgcca aaaatgattc
      601 ctgtttcatt tttttccata tagtctgtat cgagctttgc catagcaagt gcttcgcgtg
      661 ttgacatgaa tgcatacatt gccggttggt gcatacccaa acggtcacgt ctgtgaagaa
      721 aatctttaag gtcaggtgtt tctacaaaac ctgtcaatgc agaacggaat cctaaatctt
      781 ttctttcctg atcaaagata attccactct ttccctgacg taacgactct gtaacttctg
      841 caaggttctt gccgatacat gaataaatac cgatacctgt gatgactact ctttgcataa
      901 atcaggaata aatacctccg ttaatgttga tgatttcgcc tgtaatataa gaggctttgt
      961 ctgatgccag aaagctgaca acatgtgcta cttcttcagg tgtgccaaaa cgttccatcg
     1021 gaactaattt acttaattct ttttcattaa gtccctctgt catatcactc ttgataaatc
     1081 caggcgcaac agcgttgaca gtgatttttc ttttagcaac ctcaaaagca agtgattttg
     1141 ttgcagcaac aatagcacct ttggcagccg cataatttgt ttgtccggca tttcctttca
     1201 atcctgagag tgaaacaata ttgacaatac ggcctgcgcg tttgcgaagc attgggttca
     1261 caacgtttcg ggtaacatta taaaatcctt tgagtgaaat atttaaaaca tcatcccaat
     1321 ctttttctgg catccacata aaaagaccat cacgggtgat gcctgcattg tttaccaata
     1381 cactgataaa tttattttca ttctctttat gccaatggtc aagagcagca tttactgttt
     1441 cattatcctg aacattaaat ggcagcaact caccactacc tccggcattt ttaattgttg
     1501 tcaatgtttc ttcagcagct tgtttgtttg atgcatagtt gatgagaatg tgcagcttat
     1561 gatctgctgc aagctgaagt gcaatggctt tgccaagtcc tcttgatgct ccggttacta
     1621 aagcgcaatt catgatttaa agttcgtttt cttttaaaat atcaataact tttctgatgt
     1681 tttcaaactg tggaacatca tcttcaacta cggcaacctg cttacggatt aaaccatgca
     1741 gtgacaaagt gtttttgcaa agtttttgag attcttcagt acttaacaag tctattgcct
     1801 gacatacagc aagtgttagc acagcaacaa cttcatagct gttatcaatt acttttctgg
     1861 caatgatggc gctgttggtt cccatactca ccacgtcctg attgtcgttg ttgtttgaaa
     1921 tgctgtgaac ataaacagag ttgctgagcg actgattttc tgctgtagta ctcgttgcaa
     1981 gaaactgcat gccctgcaac ccaaaattaa atcctagttt tttcttgttc agaaatggag
     2041 gaaattttct gttgagtttt ttgttcaata aaaagtttag ctgacgttcg gcaagtacag
     2101 aaagcttaga aatggcaagt ttaagtttat ccatttcaag agaaatataa tcaccatgaa
     2161 agttgccgcc atgaaacaca ttgtctgctt cgggcatgac tacaggattg tcgttggttg
     2221 aatttaattc gttttcagta acggcaatag cagccatgca ggtatcataa ataggtccta
     2281 tgatttgtgg tacacagcga atggaataat attcctgaat tttgtttttg aattctgtac
     2341 ggctcagttc ggcatgatca gagaagagct cttttctgct acgcaccaat ttacttccct
     2401 tcaaaaattc gcgcatttgt tgagcaacct cacgttgtcc gttgtgtagc ttaacgtgat
     2461 tgagttcagc agaaaaagaa tcatcaaacg attcaataat ttcgttgatc actgccgata
     2521 aagatatcat ccagcgagta agtcgctttg cataaacaag gttgatggct gcaatgcctg
     2581 tcatgcacga tgtgccattg ataatgccta agccatcacg cagttgtagt gttaatggtt
     2641 ttatgtttaa cttcttaaaa acatctgctg ttttttgacg cttaccatca taatgaacat
     2701 aaccttcgcc tatcagattt agtccaaggt gtgcaagctg aaccaaatcg ccactggcac
     2761 caacgccacc atgctcataa atttcaggaa caatattttc attcagcatt gtggtgagca
     2821 atgaaattac ttcggggctt acacctgaat g
//
LOCUS       DAOOOL010000235         2806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4373_length_2806_cov_78.426390, whole genome shotgun sequence.
ACCESSION   DAOOOL010000235 DAOOOL010000000
VERSION     DAOOOL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_2806_cov_78.426390"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..795
                     /locus_tag="PKU82_12070"
     CDS             16..795
                     /locus_tag="PKU82_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOZ83643.1"
                     /translation="MSSESFEVKLPAFEGPFDLLLFFIERDELDIHNIPISKITEDFL
                     NYIHNLNALNIEVAAEFILVASTLMRIKAKLLLPRPQIDEQGNEIDPRHDLVQQLLEY
                     KKYKAAAEEFRFMEEERAMKFVRGNLAKEAAKIAVVADSGGFSSELHQLTMYKLIAAY
                     QRVLQRFELSQLDLRHTVVQYPFTLESEKESIIKKISVKASVSFEELFESCTSKIHAV
                     FIFLALLDLLQIHEVSINVGEGFNNFVLQKPQDLFTHADRE"
     gene            779..1840
                     /locus_tag="PKU82_12075"
     CDS             779..1840
                     /locus_tag="PKU82_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOZ83644.1"
                     /translation="MQIESKDVLRRLNPTKIIWPMLIGLSVAGWLFYDNYDGKVFSQI
                     DWTWHSTFWMILAGFSVFVRDLAYMIRIRVLTDNELTWYRSFIVIMLWEFSSALAPGI
                     IGGGFLFAIFILNREGINLGKSITTITFTSFLDGVFLAVMAPVVYLLVGKDALFSGVN
                     VGQTSLGTSIFYSFWVVYFIILAYKLFIAYALFVNPYLIKKILTGICSFPVLKRFRKG
                     AVETGDQLIIASNGLKDKNLKYWMLSLLATFASWTARYSIVNCLIHAFHGQTALSDLI
                     VYGKQVIMGIIILLSPTPGGSGIAELMFTDFLGEFIHKGLAPTLGILWRLISYYPYLI
                     VGALILPRWIRERIVKKIF"
     gene            1845..2057
                     /locus_tag="PKU82_12080"
     CDS             1845..2057
                     /locus_tag="PKU82_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83645.1"
                     /translation="MSNKRRIFLIVISVTIGMGLSVIIFLMKNKGKALSDMDYLWLGT
                     NLFFSLLIVGGIGLYFMKKKDNDLMK"
     gene            2093..>2806
                     /locus_tag="PKU82_12085"
     CDS             2093..>2806
                     /locus_tag="PKU82_12085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOZ83646.1"
                     /translation="MKKQTLVVALTIVFGLMTFAVQAHNEGKQVTYQAGNTNCNGYVA
                     YNAHIPKMPVVLVIPEWWGCNEYAKMRADMLASMGYFAFAVDMYGDGYIAPTPKEAGE
                     QASKFYTDNALAMERINAAIQKLKDFPMADVENMAIIGYCFGGSMALNAARANLPVKA
                     VVSFHGGLKGIANPAEIKPKILICHGGSDNFVSAEEVTAFKAEMDKVKASYRFIDYPG
                     ATHAFTNPEATAVGKKFDMP"
BASE COUNT          824 a          456 c          614 g          912 t
ORIGIN      
        1 caggcatttc tatctatgtc atcagaaagt tttgaagtta agttaccagc ctttgaaggg
       61 ccgtttgatt tgttgttgtt ttttattgaa cgcgatgagt tggatattca caacatacct
      121 atttctaaaa tcacagaaga ttttctgaac tacattcata atcttaatgc tttaaacatt
      181 gaggtggctg cagagtttat tttggttgcc agtacgttaa tgcgcataaa ggcaaaacta
      241 ttgctacccc gaccgcagat tgatgaacag ggtaatgaaa ttgacccacg acatgatttg
      301 gtgcagcagt tactcgagta taaaaagtat aaagccgctg cagaagaatt ccgttttatg
      361 gaagaagaac gtgccatgaa gtttgtgcgt ggtaatttgg ctaaggaggc ggctaaaatt
      421 gctgttgttg cagacagtgg cggattcagc agtgagttgc atcagctgac gatgtataaa
      481 cttattgcag cttatcagcg tgtgttgcaa agattcgaac tttcgcaact cgacctgcgc
      541 catacagtag tgcaatatcc ttttacatta gaatcggaaa aagaaagtat catcaagaaa
      601 attagtgtta aagctagtgt aagttttgaa gaactgtttg agagctgtac cagcaaaata
      661 catgcagttt ttatttttct tgccttgctc gatttgttgc agatacatga agtaagtatc
      721 aacgttggcg aagggttcaa caattttgta ctgcagaaac cacaggatct gtttactcat
      781 gcagatagag agtaaagatg ttctgcgcag gctgaatcca acaaaaataa tttggccaat
      841 gctcattggt ctgtcagtgg caggttggtt gttttatgat aattacgatg gcaaagtttt
      901 ttcgcaaatt gactggacat ggcatagcac cttctggatg attcttgcag gcttttctgt
      961 atttgtgcgc gatttggcgt acatgattcg cattcgggta cttacagata atgaacttac
     1021 atggtacaga agttttattg taattatgct atgggagttt tcttctgcct tagcaccggg
     1081 aataataggt ggcggatttt tatttgctat ttttattctc aaccgcgaag gaataaacct
     1141 cggtaagagt attaccacca taacctttac ctcttttctt gatggtgttt tcttagctgt
     1201 gatggcaccg gtagtttatt tgctggtagg taaggacgct ttattttcag gcgtaaatgt
     1261 ggggcaaaca tcattgggta caagcatttt ttattctttc tgggtagtat attttatcat
     1321 tcttgcctat aaacttttta ttgcttatgc actttttgta aatccatatc tgattaaaaa
     1381 aatattaacg ggcatctgtt cttttccggt cttgaaacga tttagaaaag gtgcagttga
     1441 aacaggcgat caacttatca ttgcctcaaa cgggttgaaa gataaaaatc tgaaatactg
     1501 gatgctttca ttactggcaa cgtttgcttc gtggacagca agatattcta ttgtaaattg
     1561 tctgattcat gcatttcacg ggcagaccgc attgtctgat ttgattgttt atggtaagca
     1621 ggtaattatg ggaataataa ttttacttag ccccacacct ggtggcagtg gcattgccga
     1681 actgatgttt accgattttc ttggtgagtt tattcacaag ggactggcac ctacattagg
     1741 aattttatgg cggttgataa gttattatcc ttaccttatt gttggtgcac taattttgcc
     1801 acgttggatt agagagcgta ttgtaaaaaa aatattttag ggttatgagt aataagcgaa
     1861 gaatttttct gattgtcatt tcagtaacta ttggaatggg tctttccgtc atcatttttt
     1921 taatgaaaaa taaaggaaaa gcattaagcg atatggacta tctttggtta ggaaccaatc
     1981 tgtttttttc attgctgatt gttggcggca taggacttta ttttatgaag aagaaagaca
     2041 atgatctgat gaaataagta agtattagtt aagtaattta aaaatcaaaa caatgaaaaa
     2101 gcaaacttta gtagtagcat taacaatagt ttttggcttg atgacatttg cagtgcaagc
     2161 acacaatgaa ggcaagcaag ttacctacca agcgggcaac accaattgca atggttatgt
     2221 tgcctataat gcacacattc caaaaatgcc tgttgtatta gtcatacctg aatggtgggg
     2281 ttgtaatgaa tatgctaaga tgcgtgccga catgcttgct tctatgggat attttgcttt
     2341 tgccgttgac atgtatggtg atggctatat tgcacctaca ccaaaagaag caggtgagca
     2401 agcttcaaaa ttttataccg ataatgcttt ggctatggag cgcattaacg ctgcaattca
     2461 aaaactaaaa gattttccta tggccgatgt tgagaatatg gccattatag gatattgttt
     2521 tggaggcagt atggcactta atgcagcacg tgctaattta cctgtaaagg cagttgtaag
     2581 ttttcatggt ggattaaagg gcattgccaa tccggcagag ataaaaccaa aaatattaat
     2641 ctgtcatggt ggcagcgaca actttgtttc tgcagaagaa gtgactgctt ttaaagctga
     2701 gatggataaa gtaaaagcaa gttacagatt tattgactac ccgggtgcaa cgcatgcatt
     2761 caccaatcct gaagctactg cagttggaaa aaagtttgat atgcca
//
LOCUS       DAOOOL010000236         2802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4385_length_2802_cov_42.133600, whole genome shotgun sequence.
ACCESSION   DAOOOL010000236 DAOOOL010000000
VERSION     DAOOOL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4385_length_2802_cov_42.133600"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1112
                     /locus_tag="PKU82_12090"
     CDS             96..1112
                     /locus_tag="PKU82_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOZ83647.1"
                     /translation="MPGGSGGTDIYLTEKMNNIWSKPVNLGPNVNTPYNEMFPMIWKD
                     SILYFSSEGHYNMGGLDLFYTSKDQNGWKEARNMGYPLNTSYDDFGVALNDSGNAGII
                     SSNRNSKNTMQDNLYTFTINDLRFTLEGIAVDKTTQEPVAGVIVELTNTITNKKETAI
                     TGPDGKFLFKLNPETSYTVVGTKDGYFTNTEPVTTVGKIRSESMYVKLKLELDRIIVD
                     KPIVLKNIYYDLDKWDIRPDAAIELDNLVQIMNENPAISIELGSHTDSRADDKYNMNL
                     SQKRAEAAVNYIVSKGVSQARITAKGYGETMLVNQCANKVACSEEEHQANRRTEFKVV
                     SIAK"
     gene            1317..>2802
                     /locus_tag="PKU82_12095"
     CDS             1317..>2802
                     /locus_tag="PKU82_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83648.1"
                     /translation="MMQLTTFYRNTISRLFFIAFVLITNLSSFNAAATHSSGADLQYT
                     WVSGNTFKVTVSFYRDCAGVAAPATITLNAKSTSCSRNQNYTLNLVGGTGQEITFPCR
                     TVQTKCTSSGSVNAGYQRYEYSNNVTLPQQCTDWVMSFYVCCRNCAITTLNNPCADNM
                     YIEATLNNVAAPHNNSAQFTNIPVALICVNQSFTYNHGVFDPDGDSLVYSFITPKTFN
                     TSTNTVGTVTFNPGFSSTSPLTSSPATTLNSANGDITMNPTVNGEIGVCAIIVREYRN
                     GVLIGSAIRDMQFLTRICNPNFLPTASGINGTGSFEVTVCPGANINFTISSADPNPAD
                     TIVMSWNNSISAATFNTVGAKLPVGTFNWTPTLADARTQPYTFIVIVRDNACPYAGSQ
                     TYSFIIHVPVITAAVSSPVFSGYNVACSGASTGAITATGGGGTSPYTYLWTPSGQTTQ
                     TATGLAVNTYSVTITDADGCTNSASTTLTEPPTVVADSISASTNI"
BASE COUNT          865 a          546 c          557 g          834 t
ORIGIN      
        1 ttcatttttt ctgtcaggta gatatcagtt ccacctgaac caccggacat cctgcattga
       61 acacagatgg aacgaagatg tatttcgtga gcgatatgcc cggtggttca ggtggaactg
      121 atatctacct gacagaaaaa atgaataaca tctggagtaa gccggttaat cttggtccaa
      181 atgtaaatac tccttacaat gaaatgttcc caatgatctg gaaagattca attctttatt
      241 tcagttctga aggtcattat aacatgggtg ggttagatct tttctatact tcaaaagatc
      301 agaacggttg gaaagaagct cgtaatatgg gttatccttt gaatacgagt tatgatgatt
      361 ttggtgtagc attaaacgat agtggtaatg caggaattat ttccagcaac aggaattcta
      421 agaatacaat gcaagacaac ctgtacactt tcacgatcaa tgatcttcgt tttactttgg
      481 aaggaattgc agtggataag acaacgcaag agcccgttgc aggtgtaatt gttgaattaa
      541 caaatacgat aacaaacaaa aaggaaacgg caattaccgg acctgacggt aagtttcttt
      601 ttaagttaaa tcctgaaaca tcatatactg ttgtcggaac aaaagacggc tatttcacaa
      661 atacggaacc ggtaaccaca gtaggaaaga tcagaagcga gagcatgtac gtaaagctca
      721 aacttgaact ggatagaatc attgtagata aaccaatcgt tctgaaaaac atttattacg
      781 atcttgataa atgggatatt cgtccggatg cagccataga attggataac cttgttcaga
      841 tcatgaatga gaatcctgcg atcagcatag aattaggttc tcacaccgat tctcgtgccg
      901 atgataaata caatatgaat ttgtcacaaa aacgtgcaga agctgccgtt aattacattg
      961 tttcaaaagg agtgagccaa gcaaggatca ctgccaaagg ttatggagaa acgatgttag
     1021 tgaatcaatg tgctaataaa gtcgcttgtt ccgaagagga acaccaggcg aaccgtagaa
     1081 ctgaattcaa agttgtatca atagcaaagt aaaaagtctg aaaatataca agtaaggccg
     1141 gagagatccg gccttttttt atggtctaaa gttgagattt cgattgaaat tcatgtggca
     1201 aaatgttgaa aagtgtgttt aaatgattac tgatataaga ggaagagtag gcttaagttt
     1261 tatgaacttt ccaacttatt ataatcaggc aatacagtct tataactcac acatacatga
     1321 tgcaattaac tacattttac aggaacacaa tttccaggtt gttctttatt gctttcgttt
     1381 taattacgaa tctttcttct ttcaatgctg ctgcaaccca ttcgtcaggt gctgaccttc
     1441 aatatacatg ggttagtggt aatactttca aagtgaccgt ctctttttac agagattgcg
     1501 ccggtgtcgc tgcgcctgct accatcacgt tgaatgcaaa atcaacttca tgttccagaa
     1561 atcagaatta cactttaaat ctggttggtg gaacaggaca agaaatcaca tttccgtgta
     1621 gaacagtgca gaccaagtgt acaagttcag gttctgttaa tgcaggctac caacgttacg
     1681 aatactcgaa taatgttact cttccgcaac aatgtactga ttgggtaatg agtttctatg
     1741 tatgttgtcg aaattgtgcg atcactacac ttaataaccc ttgtgccgat aatatgtaca
     1801 ttgaagcaac attgaataac gttgccgctc cacataataa ctcagcgcag tttacaaata
     1861 ttcctgttgc tttgatatgt gtgaatcaaa gtttcactta caatcatggt gtattcgacc
     1921 cggatggtga ctcattggtt tactctttta taactccaaa aacatttaat acttcaacaa
     1981 atacggttgg aactgtaaca ttcaatccgg gtttttcaag tactagtcct ttgacttcaa
     2041 gtcctgccac aaccttgaat tcagcgaacg gagatatcac aatgaatcca actgtcaatg
     2101 gagagatcgg agtttgtgcg atcattgttc gagaatatag aaacggtgtc cttatcggaa
     2161 gtgcaattcg tgatatgcaa tttctgacac ggatttgtaa tcccaatttt ttacctacgg
     2221 catcgggtat aaatggtaca ggtagttttg aagttactgt ttgtccgggt gccaacataa
     2281 actttaccat aagttcagcc gatccaaatc ctgccgatac gatcgtgatg tcatggaaca
     2341 attcaatttc tgctgcaact ttcaatacag taggagcgaa gttgcctgtt ggaacattta
     2401 actggactcc aacattagct gacgcacgta ctcagcctta tacttttatt gtcattgttc
     2461 gtgataatgc ctgcccttat gccggatcgc aaacctattc ttttatcatt catgtccctg
     2521 tcattactgc tgctgtatcc agtccggttt tctccggcta taatgttgct tgtagtggag
     2581 catcaacagg agcaattacg gcaaccggtg gtggaggaac ttcgccatat acgtatctat
     2641 ggacaccttc aggtcaaact actcaaacag caacaggtct ggcagtaaat acttattcag
     2701 taactatcac cgacgctgac ggttgtacca attccgcttc aactacactt actgagccac
     2761 caaccgttgt tgctgattct atttctgctt caacaaatat at
//
LOCUS       DAOOOL010000237         2775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4447_length_2775_cov_36.149632, whole genome shotgun sequence.
ACCESSION   DAOOOL010000237 DAOOOL010000000
VERSION     DAOOOL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4447_length_2775_cov_36.149632"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..570
                     /locus_tag="PKU82_12100"
     CDS             145..570
                     /locus_tag="PKU82_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83649.1"
                     /translation="MNKTKITNYKELLAAKEVLQKRSLDLEEKLTANINTLKSELTPA
                     NILNKTFKALLFENKAGITGSALRLGAEYLIGRSAQRKSGSALYGTLGYVAAKSLVNY
                     TLENKKYLLNTLSRLLNKIDLKKNEPNNIFDQSTADDHF"
     gene            complement(578..742)
                     /locus_tag="PKU82_12105"
     CDS             complement(578..742)
                     /locus_tag="PKU82_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83650.1"
                     /translation="MKQLLYIFITQWADKTISLVGLVLFKTSVLAGLLSFTSKKRKEN
                     YKIVIEKEKL"
     gene            957..2420
                     /locus_tag="PKU82_12110"
     CDS             957..2420
                     /locus_tag="PKU82_12110"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOZ83651.1"
                     /translation="MQTTATKDVNYEQDLNEWIKAEKAAIELIHVTGSLWFDKSVELV
                     MFRNQMIDRSSSQLLNLIQYSKEIVKKPINIFDALAIAKELNAIDLAPSRLDLGRLAH
                     EWLVEKEKYKTVNKFIADKLKDFIGKGKKTLTPKDVVLYGFGRIGRLLARELTIMAGK
                     GEQLRLRAIVTRDKSDEDIIKRADLLRTDSVHGPFPGTVIEDIQNRALIINGHTVHMI
                     SAKNPEDIDYTKYGINNALLVDNTGVARDREGLGKHLLSKGVDKVLLTAPGKGDIPNV
                     VYGVNQTQHDANEKIYSAASCTTNAIVPVLAVINKTLGIERGHIETIHSYTNDQNLLD
                     NYHKKYRRGRSAALNMVITETGADKAVSKALPELAGKITGNAVRVPTPDVSLAILNLT
                     LGKKSDKDSINEILKDAALKGDLVEQIQYSYGNELVSTDLVGNPCASIVDSPATIVSK
                     DGKTAVLYVWYDNEYGYSRQVIRFAKYLADVIRLTYY"
     gene            complement(2477..>2775)
                     /locus_tag="PKU82_12115"
     CDS             complement(2477..>2775)
                     /locus_tag="PKU82_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83652.1"
                     /translation="TCPCLITGINEIKQHNFKFSVTPNPTNGNFKIVYLLPQDKSGTL
                     QIFDITGKEVYKQNLPPWSTMQYISLPKLANGVYYCTITSNNERVHKKLVVFKE"
BASE COUNT          939 a          446 c          532 g          858 t
ORIGIN      
        1 gcatatatgc aggctttttt attatcgcat tattctttct tattgtattg gtttgtatag
       61 ctgtgttttc acaaagactg attaaagaac cgttaactga ttttattatc agacgactaa
      121 atggttccga aaaaaatgct gctgatgaat aagacaaaaa ttacaaacta caaagagttg
      181 ctggcagcaa aagaagtctt gcaaaaacgc agtttagatt tagaagaaaa actaactgcc
      241 aacatcaata cattaaaaag tgagttgaca cctgccaata ttctgaataa aacatttaaa
      301 gcattattgt ttgaaaacaa ggcaggtatt acgggtagtg cattaaggct tggtgcagaa
      361 tatttaattg gcagaagcgc acaacgtaaa agtggttcag cattgtatgg tacgttagga
      421 tatgtagctg caaaatcact ggtcaactac actcttgaaa acaaaaagta tttgctcaat
      481 acattaagta gattgctaaa taaaattgac ttgaaaaaaa atgagccgaa caatattttc
      541 gatcaatcaa cggctgatga tcatttctga ttcactctta aagcttttct ttttcaatca
      601 caatcttata gttttctttt ctctttttgg atgtaaaact taacagacct gcaagtacag
      661 aagttttaaa taataccaat ccaacgagac tgattgtttt atctgcccac tgcgtaataa
      721 atatgtataa aagctgtttc atccacctta aaattctgac tacaaaagtc catcaattag
      781 cccattaaac ggattaaaca gaaaaatgtt tttaacatta tgttgtgtag ttaatctgca
      841 attcatttta aagtactgaa tcacaaagca gttataatgt aataaatggt gcaattttgc
      901 tgaaaaatga ttgtgaatat gaagtatatt tgcagcctga ataaaatcaa acaaaaatgc
      961 aaacaacagc aacaaaagat gtaaactacg aacaggatct gaacgaatgg attaaggccg
     1021 aaaaagctgc cattgagttg attcatgtta caggatcttt atggttcgat aaatctgtag
     1081 aattggttat gtttcgcaat cagatgattg atcgcagtag cagccaattg ttgaacctga
     1141 ttcagtattc taaggaaatt gtaaaaaagc cgattaatat ttttgacgct ttagctattg
     1201 ccaaagagtt aaacgctatt gatcttgcac catcacgtct cgatttagga cgtttggcgc
     1261 atgaatggct cgttgaaaaa gagaaataca aaacagtaaa taagtttatt gctgataaac
     1321 taaaagattt tatcggtaag ggcaaaaaga cattaacacc taaggatgtt gttctttatg
     1381 gttttggtcg gattggcagg ttactggcgc gcgagctaac tatcatggct ggtaagggcg
     1441 aacagcttcg tttacgtgca atagttaccc gtgataaaag tgatgaagat ataatcaaac
     1501 gtgcagattt gttacgtaca gatagtgttc atgggccatt tccgggcact gtgattgaag
     1561 atattcaaaa ccgtgctctc ataattaatg gacataccgt acacatgatt tcagcaaaga
     1621 atcctgaaga tattgattat acaaagtatg ggattaacaa tgcgctgctg gttgataata
     1681 ccggtgttgc acgtgaccgt gaaggcttag gtaaacattt actttcaaaa ggagttgata
     1741 aagtattgtt aacagctccc ggcaaaggag atattcctaa tgttgtttat ggtgtaaatc
     1801 aaacacagca tgatgcaaat gaaaaaatct attctgcagc atcttgtact accaatgcca
     1861 tagttccggt gttggcagtt attaataaga cattaggtat cgaacgtgga catattgaaa
     1921 caattcactc ttatacaaac gatcagaatc ttctcgataa ctatcataaa aaatacagac
     1981 gaggtcgttc ggccgcttta aatatggtaa ttactgaaac aggtgcagat aaagcagtgt
     2041 caaaagcttt acctgaattg gccggtaaaa ttacaggcaa tgctgtaagg gttcctactc
     2101 ctgatgtatc cttagcaatt ctgaacctga cattaggaaa aaaatcagat aaagattcca
     2161 tcaatgaaat cctgaaagat gcagcactta aaggtgattt ggttgagcaa attcaatact
     2221 cgtacggtaa tgaattagta agcaccgacc ttgtgggtaa tccatgcgca agcattgttg
     2281 atagtcctgc tactattgta agtaaagacg gaaaaacagc agtgctttat gtttggtatg
     2341 ataacgaata tggctatagc cgtcaggtaa ttcgttttgc aaaatatctt gctgacgtta
     2401 tcagactgac atactattaa gttttaagat ttacaaaaaa caaaaggctt tctctaaatg
     2461 gggaagcttt tttttactac tccttaaaga caactaactt tttatgcact ctttcattat
     2521 tgcttgtgat tgtgcaataa taaactccgt ttgcaagttt tggtaaggat atatattgca
     2581 ttgtactcca cggtggcagg ttttgtttgt aaacttcttt acctgtaata tcaaaaattt
     2641 ggagtgtacc acttttgtcc tgtggcaaga gataaactat tttgaagttg ccgtttgtag
     2701 ggtttggtgt aacagaaaac ttaaaattgt gttgcttaat ttcatttatg cctgttatca
     2761 aacaagggca agtgg
//
LOCUS       DAOOOL010000238         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4514_length_2748_cov_17.424062, whole genome shotgun sequence.
ACCESSION   DAOOOL010000238 DAOOOL010000000
VERSION     DAOOOL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4514_length_2748_cov_17.424062"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKU82_12120"
     CDS             complement(<1..1061)
                     /locus_tag="PKU82_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83653.1"
                     /translation="MIALVVVGSLSASAQLTRKTAVGKQHQKQVPANLNAARHSSQTN
                     STNAVIWSDDFSTPANWSIAHEAGTTGDWAIGTTGPSGSFPINTITSASAANGFAIFD
                     SDLICSGNQIGNLSTVNPIDLTGHASVRLTFSQYYCRYYDSTYVFVSTDNTNWTKFEV
                     NGNLVINDFSANNNSAVNPEIVNVDISSVAGNSATVYIRFQFYSPSTIDVSAGCAYAW
                     MLDDVSISDIPAIDAAAYNKAFAGEYSIMSLLNTTAFTMNGRIVNTGGTAITGGTIAF
                     NVYNASGQVYNDLATLSSTLNPGDTSAVLTSAGSYAPADTGIYIIEQIIGVSGDGDAA
                     NDTIFAFVYVDDSTNARDYT"
     gene            complement(1377..>2748)
                     /locus_tag="PKU82_12125"
     CDS             complement(1377..>2748)
                     /locus_tag="PKU82_12125"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83654.1"
                     /translation="LITDAIDCSAHSNVHLFFNELLAHYAEIPTVSVSTDGSTWTQVF
                     DAAAGLNQNDVTPNPTAIDIDISAIAANQVTVYIKFNFTGDYDYFWMVDDITLYEQEG
                     TDAFLTSITAPTNSCTLLSATEVIEVSIYNNGGTDITQFDITYIADGGAPSLETVTTL
                     IAPGATFSYPFSATADFSLAGAHTIQAFITVLGDTTQTNDTVNASFFTGPHSIPTSPG
                     YSNGFELTDDMSGFATEDLNNDSITWDLSNVLPNTGTYCARISAATAEDYLFTTCFEL
                     VDTAEYTLSYFYRATSTSTPTFFEIVLATDQNSSSITQIISPIALISNLAYLPGAAQI
                     DVATTGTYYIGFHAVSGDSLAGLRLDDINITGAAGVGINSIQAGKTVVYPNPSTGFIY
                     LNSTVNSASFNVEVINALGQVVFAKKYGQLSAEMIDLSNQSAGQYIVKVINDKGVNTQ
                     VVNITK"
BASE COUNT          843 a          512 c          518 g          875 t
ORIGIN      
        1 gtatagtcac gtgcattagt tgaatcgtca acatatacaa atgcaaaaat agtgtcattt
       61 gctgcatcgc catcaccact aacaccaatg atttgctcaa tgatgtagat acctgtatct
      121 gccggagcat atgaacctgc tgaagttaaa actgctgatg tatcaccagg gttcagtgta
      181 gaagaaagtg tcgcaagatc attataaacc tgacctgaag cattgtaaac attgaatgct
      241 atcgtaccac ctgtaattgc tgtaccgcct gtattcacaa tacggccatt catagtgaat
      301 gctgttgtat tcaaaagaga catgattgaa tattcacctg caaatgcttt gttataagca
      361 gctgcatcaa ttgcaggaat gtcagaaata ctaacatcat ctaacatcca agcgtaagca
      421 caacctgcag atacgtcgat tgtactaggg ctatagaatt ggaaacggat ataaactgtt
      481 gctgaattac cggcaactga agagatgtcc acattaacaa tctccggatt aacagcgcta
      541 ttgttgtttg cactgaaatc attgatcact aagtttccgt ttacttcaaa ttttgtccag
      601 tttgtgttgt ccgtacttac aaatacataa gttgaatcat aataacggca atagtactga
      661 gagaaagtta atctaacaga agcgtgaccg gtaaggtcaa ttgggttaac tgtagataga
      721 tttccaattt gatttcctga acagatcaag tcagaatcaa aaatagcaaa accattagct
      781 gctgatgcag aagtgatcgt gttgataggg aatgaaccac ttggtcctgt tgttcctatt
      841 gcccagtcac ctgttgttcc cgcctcatga gcgattgacc aatttgcagg tgttgagaaa
      901 tcgtcactcc aaattactgc attagtagaa tttgtttgtg aactgtgacg tgctgcattt
      961 aaatttgcag gtacttgttt ttggtgctgt tttccaacag cagttttgcg agtcaattgt
     1021 gcagaagccg ataagctacc aacaactact aacgcaatca ttgcgtataa ttttttcatc
     1081 attaatttgg ttagagattt acgaattgca aagttatgtt ttttcttagt ttgagcaatt
     1141 tgtttctcct tttttaacca aatttttgtc atattattcc tgccattttt cgttaaattg
     1201 taatatagaa tttgccgaat atttggtaaa ggcttatttt atagccactt cactttaaac
     1261 tggtagactt tcaatcatta ctccaaagcc aacaggccta tgtgtcaatt cggagttctt
     1321 tttgcataaa aaaaggctgc cttttggagg cagcctttca tataaagtaa ggttgattat
     1381 ttagtgatat tcaccacttg tgtgtttaca cctttgtcgt ttataacttt tactatatac
     1441 tggccggcag actgattgct caggtcgatc atttctgcag aaagttgtcc atatttctta
     1501 gcaaatacaa cctgtccaag agcattgata acttcaacat tgaaagaagc agaattaaca
     1561 gtactgttca gatatataaa tcctgtagat ggatttggat atacaacagt ttttcctgct
     1621 tgaatagagt tgattcctac tccggctgca cctgtaatat tgatatcgtc aagtcttaat
     1681 cctgcaagtg aatctccact tactgcatgg aaaccgatat aatatgttcc tgtcgttgct
     1741 acatcaattt gagctgcacc aggtaaataa gcaagattag aaattaatgc aatcggagaa
     1801 ataatttgag taattgatga actgttttga tcagttgcta atacgatctc aaagaatgtt
     1861 ggtgttgaag tactggtagc acgatagaaa taactaagtg tgtattctgc agtatcaact
     1921 aattcaaaac atgttgtgaa taagtagtct tctgcagttg cagcagaaat tctagcacaa
     1981 tatgttcctg tgtttggaag tacatttgaa agatcccacg taatagaatc attattcaga
     2041 tcttcagtcg cgaaacctga catatcatct gttaattcaa aaccgtttga ataacccgga
     2101 cttgttggaa tactgtgtgg tcctgtgaaa aacgaagcgt ttacagtatc gtttgtttga
     2161 gtcgtatcac ctaacacagt aataaatgcc tgaatcgtgt gagcccctgc taaagaaaaa
     2221 tctgctgttg cagaaaatgg gtaagaaaaa gtagcacccg gtgctattaa tgtcgtaacc
     2281 gtttcaagtg aaggtgctcc tccatctgca atatacgtga tatcgaactg agtaatatct
     2341 gttcctccat tattgtaaat agaaacttct attacctcag tagctgaaag tagtgtacag
     2401 ctgtttgttg gtgctgtaat gctcgttaaa aacgcatctg taccttcttg ctcataaagc
     2461 gtaatgtcat ctaccatcca gaaataatcg taatctcctg taaagttgaa tttaatataa
     2521 accgttacct gatttgcagc aatcgcactg atatcaatat caattgctgt agggtttgga
     2581 gttacatcgt tctgattcaa tcctgcagca gcatcaaaca cctgagtcca tgtagaacca
     2641 tctgtactta ctgaaactgt tggaatctct gcataatgtg ctaacaattc attgaagaaa
     2701 agatgtacat tactatgtgc tgaacaatca atagcatcag tgataaga
//
LOCUS       DAOOOL010000239         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4520_length_2746_cov_85.813081, whole genome shotgun sequence.
ACCESSION   DAOOOL010000239 DAOOOL010000000
VERSION     DAOOOL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4520_length_2746_cov_85.813081"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKU82_12130"
     CDS             <1..745
                     /locus_tag="PKU82_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HOZ83655.1"
                     /translation="LKKKMKVFKKCISLFSCCMLVLNFSFGQINYGKITYERKTNLYK
                     RFKSDSDVKEWLKEEDKNKIDIFELYFNDTASVFKPQESNLVERMSWATNKNVVYQNF
                     KTNTRFTEKKIWGEQFYLIDSVRKFTWKITDSKRNICGFQCRKAIWQVNDSTRIYAWF
                     CSEIIPSIGPESILGLPGAILGLATEDGGVIYFAKNVEILKPDATLLAPKKNKQKIYT
                     TDELKTKIEKDFGKEKWGKSLLYNQFAIW"
     gene            complement(868..1254)
                     /locus_tag="PKU82_12135"
     CDS             complement(868..1254)
                     /locus_tag="PKU82_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HOZ83656.1"
                     /translation="MNQSPMRAALKLKCPKCAQSNLFTDPNPYNFKKLGDMPTVCPTC
                     ALPFTPEPGFYYGAMYVSYALTVGMSVFNFIWVYMLWGFVVAPYLIINSILLIALFPL
                     FFRYSRSLFLAMVFKIEEAARKRQRH"
     gene            complement(1254..1976)
                     /locus_tag="PKU82_12140"
     CDS             complement(1254..1976)
                     /locus_tag="PKU82_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOZ83657.1"
                     /translation="MEQIRFKQPDIGAQVLFEDNHLIAINKLPGQITQGDKTGDVPLG
                     DLVKNYLKEKYQKPGNVFCGTIHRLDRPVSGVVLFAKTGKALERMNEQFRERKVQKTY
                     WAIVKNKPQKVEDILVHYLQKNETKNKSFASLQPKPDATRCELSYKIIAASDHYHLLE
                     VNPLTGRHHQIRVQLSAMGCPIKGDLKYGADRSNADGSISLHAHRLTFVHPVKSEPIE
                     INALPPDEPLWNFFVQQLSNTK"
     gene            complement(1976..>2746)
                     /gene="panB"
                     /locus_tag="PKU82_12145"
     CDS             complement(1976..>2746)
                     /gene="panB"
                     /locus_tag="PKU82_12145"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOZ83658.1"
                     /translation="QEMKRSGEKISMLTAYDFSLAGIIDDAGIDVILVGDSASNVMAG
                     HETTLPITLDQMIYHASSVVRAVKRALVVVDLPFGSYQGNSKEALQSAIRIMKESGAH
                     AVKLEGGREVKESIERILTAGVPVMGHLGLTPQSIYKFGTYVVRAREEEEAKRLIEDA
                     HILEEAGCFAIVLEKIPAALAATVAQEVKIPVIGIGAGNGVDGQVLVLHDMLGINNEF
                     HPRFLRRYLNLYDEIKAATAKYITDVKSRDFPNSKEQY"
BASE COUNT          888 a          520 c          479 g          859 t
ORIGIN      
        1 tctaaagaag aagatgaagg tttttaaaaa atgcatttca ttgtttagtt gttgcatgct
       61 cgtactgaat ttttcattcg gccaaataaa ttatgggaaa attacctatg aacgtaaaac
      121 aaatctatat aaacgattca agagtgatag tgatgtaaaa gaatggctga aagaagaaga
      181 caaaaataaa attgacattt ttgaattata ttttaatgat actgcatcgg tttttaaacc
      241 acaagaaagt aatttagtgg aacgtatgag ttgggcaaca aataaaaatg tggtgtatca
      301 aaactttaag acaaatacac gttttactga aaagaaaatc tggggtgaac aattttattt
      361 aattgattcc gtgagaaagt ttacttggaa aattacagat agtaaacgca atatttgtgg
      421 ctttcaatgt agaaaagcta tatggcaagt taatgacagt accagaattt atgcatggtt
      481 ttgctctgaa atcattccct ccattggacc tgaaagtata ttaggactac cgggtgcaat
      541 acttggatta gcaactgaag atggtggtgt tatttatttt gcgaaaaatg tagagattct
      601 aaaacctgat gcaacattgt tggcacccaa aaaaaacaaa cagaaaattt atacaactga
      661 tgaacttaaa accaaaattg agaaagactt tggaaaagag aaatggggaa aatctttgtt
      721 atataaccaa tttgcaattt ggtaaaatct aaaaagaagt catttatttt atctgacaac
      781 atcttaaatc aaacacaatt aatttgcatt ctgacattca ttttaatgct ctatttcttc
      841 actcacaaat tacacttaca aaaatattca atgccgttgt cttttccttg cagcttcttc
      901 aattttaaaa accattgcca aaaataaact tcgtgaataa cgaaagaaaa gtggaaacaa
      961 tgctatcaat aaaatactat ttataattaa atagggagca acaacaaatc cccataacat
     1021 atacacccaa ataaaattaa ataccgacat gccaacagtt aaggcatagc ttacatacat
     1081 ggcgccatag taaaaaccag gctccggagt aaatggcaat gcacatgttg ggcacactgt
     1141 tggcatatcg cccaacttct taaaattata tggattgggg tctgtaaaaa gatttgactg
     1201 tgcacattta gggcatttta attttagtgc agcccgcatg ggtgattgat tcattattta
     1261 gtgttggata attgttgaac aaaaaaattc cataaaggtt catcaggagg taaagcattt
     1321 atttcaattg gctctgactt tacagggtgg acaaaagtca gcctatgagc atgtaggctt
     1381 atggagccat cggcattgct gcggtcggca ccatatttta aatcgccttt tattggacaa
     1441 cccattgcag agagttgcac tctaatctga tgatgtctgc ccgttaacgg attcacttca
     1501 agcaaatgat aatgatctga tgcagcaata attttgtagg ataactcaca acgtgttgca
     1561 tcaggtttag gttgcaagga agcaaaactc ttattcttgg tttcattctt ttgcagataa
     1621 tgaactaaaa tatcttccac tttctgtggt ttgtttttta ctattgccca ataggttttc
     1681 tgcacttttc tctctcgaaa ttgctcattc atacgctcaa gtgcttttcc tgtcttagca
     1741 aatagcacaa caccgctaac agggcggtca agacgatgga tagttccaca aaatacattg
     1801 ccgggtttct gatatttttc tttcagataa tttttaacca aatcgcctaa tggcacatca
     1861 cctgttttat caccttgtgt tatctgcccc ggaagtttat tgatagcaat tagatggttg
     1921 tcttcaaata aaacctgtgc gccaatatcc ggttgtttaa atcggatttg ttccattaat
     1981 actgctcctt gctattggga aaatcgcgac tcttaacgtc tgtaatatat tttgccgtag
     2041 ccgctttgat ttcatcatac aaattgagat aacgtcttaa aaaacgtgga tgaaattcat
     2101 tgttgatgcc cagcatatca tgcaatacca atacttgtcc gtcaacaccg ttgcctgcac
     2161 caatgcctat aacaggaatt tttacttcct gcgcaactgt tgctgccagt gcagcaggaa
     2221 ttttttcaag cacaatagca aaacatcctg cttcttctaa aatgtgtgca tcttcaatca
     2281 atcgttttgc ttcttcttct tcgcgtgcac gaaccacata ggttccgaat ttataaatag
     2341 actgtggtgt taaacccaaa tgacccatca caggaacgcc tgctgtcaaa attctctcta
     2401 tagactcctt tacttctctt ccgccttcaa gttttactgc atgtgcacca gactctttca
     2461 tgattctgat agcagactga agcgcttctt tactgttgcc ctgataggaa ccaaaaggca
     2521 aatcaactac aaccaaggct cgttttactg cacgcacaac acttgatgca tgatatatca
     2581 tctgatcgag tgtgataggc aatgtggttt catgccctgc cattacatta gatgcagaat
     2641 caccaactaa aatgacatca atacctgcat catcaattat tcctgcaaga ctgaaatcat
     2701 aggctgtgag catagatatt ttttcgccac tgcgtttcat ctcctg
//
LOCUS       DAOOOL010000240         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4584_length_2721_cov_52.974119, whole genome shotgun sequence.
ACCESSION   DAOOOL010000240 DAOOOL010000000
VERSION     DAOOOL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4584_length_2721_cov_52.974119"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKU82_12150"
     CDS             complement(<1..461)
                     /locus_tag="PKU82_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83659.1"
                     /translation="MKKLILIALPIVGMTLFGCENYKEQAAQLTRERDSLVSLNVAGT
                     QTIDEFITTLTDVENSLGKITEKQNSIAMTADRNPEAARNSRERLNAQIEEINRMMQE
                     NKDKVEQLSKKLKGSNASAAKFQKMVKSLQEQLTQKEQELALLNEKLAGLN"
     gene            688..>2721
                     /locus_tag="PKU82_12155"
     CDS             688..>2721
                     /locus_tag="PKU82_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83660.1"
                     /translation="MQKRLYIVILMLLALAVAFFLAWFNYQNTTALSRTITLLQKPDT
                     RLSDLKELILNVNLAESHVRSFAITRNEKNLESYYKQVEQTDSQLVKLKNLFPANSQA
                     FDSLTASIHYKFDIYDKLIELRYRQLINTAMSKISDKVKDVDVYQNTDSVETSKPGFF
                     KKLFSSGKKIKELEEKAMLLDSLNNLQAQKLSTIKQTISEAKENEIKMVAQLSDGELL
                     LLEQDRMLKEKIQSQIALLEENIRHNNENRSLEVISQSQKQLKTVVIATITASVLILI
                     LSIIILSDISRANRYKIQLEEARARSEQLARFKEEFLATMSHEIRTPLSALTGFINRL
                     QQTNTTSEQRQYVNTINMAGQHLLNIVNDVLDLSRIETGKLNFSQEPFVIYKEVNDVV
                     TLLRQKADEKNIALILEASTIRNITVSGDALRLRQVLINVIGNAIKFTEKGTVFVKVK
                     KQADDFFNFEITDTGIGIAEEKIAKLFDAYQQADNIERNYGGTGLGLAITKKLVELQK
                     GTISMKSVPEKGTTVSIMLHFPLTKQTETNIAETEFVSLHGLRLLVAEDDVLNRQLIS
                     AMLKDSGAEVITAESGKQAIEKILLYNPDGVLMDIQMPELDGRNATSFIREHISAMLP
                     LIGITANMMNDAKNECIAAGMNAVILKPFKLHELSEILLPLIDKNREVQHKPLL"
BASE COUNT          906 a          449 c          517 g          849 t
ORIGIN      
        1 atattcagtc cagctaattt ttcattcaac aaagcaagtt cttgttcttt ttgagtaagt
       61 tgctcctgaa gcgacttaac cattttctga aattttgctg cagaggcatt tgagcctttt
      121 aatttttttg acaactgctc aactttatct ttgttttcct gcatcatacg attgatttct
      181 tcaatctgtg cattgagtct ctcgcgtgag ttgcgtgctg cctccggatt acggtctgca
      241 gtcattgcaa tagaattttg tttttctgta atcttgccaa gactgttttc tacatcagtc
      301 agagttgtga taaactcatc aatagtttgt gttccggcaa cattcagtga tactaaagaa
      361 tcgcgctcgc gagttaactg tgcagcttgt tctttatagt tttcgcaacc aaataaggtc
      421 attcctacta ttggtaatgc aattaaaatt agctttttca ttgtaaatga ttttatattg
      481 tttaacatca atgcactatt aaataatgtg ccactgcttc cgtttgagtt taataaagag
      541 gtttttcagc atttaaagac tttaccaatc aatatgttat aaagttatta acgaacaaac
      601 tatgtaagtt tgatttctga taaattgttt cattttggga aaaatattcc accattggga
      661 gatttagcgt tgtatttttg actcattatg cagaaacgat tgtatatagt tatcctcatg
      721 ttgcttgctc tggcagtagc attttttctg gcttggttta attatcagaa tacaacagca
      781 ctcagcagaa caattacgct actgcagaag cccgacacaa gactttctga cctgaaagag
      841 cttatattaa atgttaacct tgcagaaagt catgttcgct cttttgcaat tacacgcaat
      901 gaaaaaaatt tagaaagcta ttataaacag gttgagcaaa ccgattcgca attggttaaa
      961 ctaaaaaatc tttttccggc aaacagtcag gcattcgact cactcactgc atctattcat
     1021 tataaatttg acatttacga taagttgatt gagttgcgtt acagacagct tatcaatact
     1081 gcaatgagta aaatttctga taaggttaag gatgtagatg tttatcagaa tacagatagt
     1141 gtagaaactt caaagccggg attttttaag aaactgtttt cctcagggaa aaagattaaa
     1201 gagcttgaag aaaaagctat gttgctcgat tcattaaata atttacaagc acaaaagtta
     1261 agcaccatca aacaaacaat ctctgaagct aaggagaatg agattaaaat ggtagctcaa
     1321 ctcagtgatg gtgaattgtt gttgcttgag caagacagaa tgttgaaaga aaagattcaa
     1381 tcacagattg cattgctcga ggaaaatatc agacacaaca atgagaacag gtcgctggaa
     1441 gttatcagtc agtcgcagaa gcaattaaaa actgttgtaa tagcaacaat tacagcttca
     1501 gttcttatat taatactttc cataataatt ctttcagata tcagtcgtgc aaacagatat
     1561 aaaattcagc ttgaagaagc acgggcacga tcagaacaac ttgcacgatt caaagaagag
     1621 tttctggcaa caatgagtca tgaaatcaga acaccattaa gtgcattgac gggctttatc
     1681 aacaggctac aacagaccaa cacaacatca gagcaacgac aatatgtgaa tactatcaac
     1741 atggcagggc agcacctgtt gaatattgta aatgatgtat tggatctttc aagaattgaa
     1801 accggtaaat taaatttttc acaagagcct tttgttattt ataaggaagt aaatgatgtg
     1861 gtaactttat taagacagaa ggctgacgaa aaaaatattg cattaatttt agaagcaagt
     1921 actatccgga atattactgt ttccggtgat gcattacgat taaggcaagt gctcattaat
     1981 gtaattggca atgcaataaa gtttactgaa aaaggaacgg tatttgttaa agtaaagaag
     2041 caagctgatg atttttttaa tttcgaaata acagacaccg gtattggtat tgccgaagaa
     2101 aagattgcaa aattgtttga tgcctatcaa caagccgata atattgaaag aaattacgga
     2161 ggcacaggtc ttggactggc aattacaaaa aaattagttg agctgcagaa aggaacaata
     2221 tccatgaaaa gtgttcctga aaagggaact actgtaagta tcatgctgca ttttcctttg
     2281 acaaaacaga ctgaaacgaa tattgcagaa acagagtttg tatcactgca tggattgcgt
     2341 ttgcttgtag cagaagacga tgtgcttaac agacaattga tttctgccat gctgaaagat
     2401 agtggcgcag aagttataac tgctgaaagt ggtaaacagg caattgaaaa aattttgttg
     2461 tataatcctg atggtgtgtt aatggatatt caaatgcctg aacttgatgg gcgcaatgct
     2521 acttctttta ttcgtgagca tattagtgcc atgcttccgc taattggcat caccgccaat
     2581 atgatgaatg atgcaaagaa tgaatgtatt gccgcaggaa tgaatgctgt tattttaaag
     2641 ccgtttaaac tccatgaact atctgaaatt cttttacctc tgatagataa gaatcgtgag
     2701 gtgcagcata aaccattgtt a
//
LOCUS       DAOOOL010000241         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4601_length_2716_cov_40.900789, whole genome shotgun sequence.
ACCESSION   DAOOOL010000241 DAOOOL010000000
VERSION     DAOOOL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_2716_cov_40.900789"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PKU82_12160"
     CDS             complement(<1..157)
                     /locus_tag="PKU82_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83661.1"
                     /translation="MPIFENILLALHAVKSQLLRTILTVLIIAIGIMALVGILTAIEG
                     LKASINEN"
     gene            656..1342
                     /locus_tag="PKU82_12165"
     CDS             656..1342
                     /locus_tag="PKU82_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83662.1"
                     /translation="MKNQIQATALAIVICLFTFFTRAQSQSVNIALTLPTDTIGACES
                     NVYALSFSGASGARVVITASLSNAPPANCINASPIYLDFITASNNVSNVVTYNNGQTM
                     SFTVTNNNAVTLSYHAFIDCSVISDAPQGNSNINFIQTFTDSSATNYTLSVNNQGNVH
                     TSLNVQTPTLVALSSNLNQVAYYKTDSFLYFYYQNTGFTKLNFNFTFTPDATNYCQRI
                     TTGQPDIILG"
     gene            1297..>1908
                     /locus_tag="PKU82_12170"
     CDS             1297..>1908
                     /locus_tag="PKU82_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83663.1"
                     /translation="MPTDYNRATGYNIGLNGNFVATAPNTAMVLNIGDTLVVRQAVQA
                     VACLDKIPACGPVAAQLQWTCNNNIPSNIFCTNCKNGIPVNYYINSGDAPVVKIERIA
                     PADSLYDFSCLNDTVNFVHWQYRVTNSGMGAMDSLFFNINQSFPTLNNALPNYNALSL
                     IPFPSFSWQQNTGSLCTVAVDTLHRSDWLCKNLVADALFEAAFR"
     assembly_gap    1909..1918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1919..>2716
                     /locus_tag="PKU82_12175"
     CDS             <1919..>2716
                     /locus_tag="PKU82_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83664.1"
                     /translation="NADTVIIAFNTLRCSEEDTNLFNVQKNYNHWAFNSVKGRKVCGD
                     GVSADYNIGLANTTSISTHAVGGSADVSQNLIFSPTVTDLSVAPGATFGDSALLKVRL
                     KGLLPSPYDYQLYGCSVPVNNCLMNGWLRAEVHCDTNLRIANPAMHGQLVYYRTSQGD
                     TLVIKPDYVFAAVDTNLCEAGNYNYYFNMNSAGFKAFLDSAHFEFTVQACCRIDKGAT
                     PYQINFYTLPNPSNCFTLTQTTPNTLPVISDVRQQWLPLSYTGDQIS"
BASE COUNT          818 a          629 c          493 g          766 t
ORIGIN      
        1 agttttcatt gatggatgcc ttaaggcctt cgatggctgt aagtattcct accaaagcca
       61 ttatgcctat ggcaatgatt aacactgtca gtattgttct gagcaactga gactttacag
      121 catgtagagc caaaagtata ttttcaaaaa tgggcatggc gctaaattac gattaatctt
      181 tgccccgaaa agtttttaac tcataaatga aagttaattt tttttcaaaa aaatttaagt
      241 tttgaaaatt caaacatata tgacgaagaa ttttccagag ttaatgtgaa caattaaata
      301 aaaatttgct agtttcaata atattgccca cttttacaaa gcggtaaatg attgccgcac
      361 aaaacaaagt tgaatcataa aaatgaagaa ataaagtaga ataaaattgt aaaaaaaata
      421 taagcgtttc gctttaaaat tcctgtcata gaaatcttgc aaaattttaa cagcacagga
      481 gagtaaaagt ggaaacgcct gcgtaaaacg cgggcgttcc ttttgcttcg tgctgtaggt
      541 ttgcaagaac ctctatgacg agcatgggaa ttagcccgcg ttttaatttt taaccacccc
      601 catacaaaat ccaaaaaatc attgtaattt agtcacaccc caaaaaccca ataatatgaa
      661 aaaccaaatc caagcaactg cattggccat tgtaatctgc ctgtttacct tctttacaag
      721 agcgcaatca caatcagtaa atattgcctt aacactgcct accgacacca taggtgcctg
      781 cgagagcaat gtttacgccc tttcattttc cggagcttca ggtgcaaggg tagtcattac
      841 tgcatcgctt agcaatgcac caccggcaaa ttgtatcaat gcaagcccca tctatctgga
      901 ttttattact gcatcaaaca atgtgagcaa tgtagttacc tacaacaacg gacaaactat
      961 gagttttacc gtaaccaaca acaacgctgt aacactatcc tatcatgctt ttattgactg
     1021 ctctgttata tccgatgcac cacagggaaa cagcaatatt aattttatac aaacatttac
     1081 agattcctct gccactaact atactttatc tgtcaacaat cagggcaatg tgcacacctc
     1141 tctcaatgtg cagacaccca ctcttgttgc cttatcatcc aacctcaacc aggttgccta
     1201 ctacaaaaca gattcttttc tttatttcta ctatcagaat accggattca ccaaactcaa
     1261 cttcaacttt acttttacac ccgatgcaac aaactattgc caacggatta caacagggca
     1321 accggatata atattgggtt aaacggcaac tttgttgcaa cagcacccaa cactgctatg
     1381 gtgctgaaca ttggcgacac gcttgttgtc aggcaggctg ttcaggctgt tgcatgttta
     1441 gataaaatcc ctgcctgcgg gcctgtagct gcgcagttgc aatggacatg caacaacaac
     1501 attccatcaa atattttctg caccaattgc aaaaatggta tccccgtaaa ctactacatt
     1561 aacagtggcg atgcaccggt tgtgaaaata gaacgcatag cacctgccga ctctttgtat
     1621 gacttcagtt gcctgaacga tacggtaaac tttgttcact ggcaatatcg tgttaccaac
     1681 agtggcatgg gagccatgga ttctttgttc tttaacataa accaatcctt tccaacgctc
     1741 aataatgcct tgcctaatta taatgcactg tcactaattc cgttccctag ttttagctgg
     1801 cagcaaaata caggaagcct ttgcactgtt gctgtggata ccctgcaccg cagcgactgg
     1861 ctgtgcaaaa atttagttgc cgatgccctg tttgaagcgg ctttcagann nnnnnnnngc
     1921 aatgcagata ccgttattat tgcttttaat accctgcgtt gctccgaaga agataccaac
     1981 ctgtttaacg ttcaaaaaaa ttataaccac tgggcgttta attctgttaa gggaagaaaa
     2041 gtttgtggcg atggtgtgtc tgcagactat aatataggct tagccaacac tacgtccatc
     2101 agcacccatg ccgttggtgg gtcggcagat gtttctcaaa atttaatttt cagccccacg
     2161 gtgacagacc tttccgtagc accgggtgcc acctttggcg acagtgctct tttaaaagtg
     2221 cgtttaaaag gactcttgcc ctcaccttat gattatcaac tttatggttg cagtgtgcct
     2281 gtaaacaact gcctgatgaa cggctggctc agagcagaag tacactgcga caccaacctt
     2341 cgcattgcca atcccgctat gcatgggcag ttggtttact atcgtacttc gcaaggcgac
     2401 actcttgtta taaaacccga ttatgttttt gctgctgtgg acaccaacct ttgtgaagcc
     2461 ggaaactaca actactattt taacatgaac agtgcaggct ttaaagcatt tttagacagt
     2521 gcccattttg aatttaccgt gcaggcctgc tgcagaatag ataaaggagc aacaccctat
     2581 caaatcaact tttatacctt gcccaatccg tcaaactgtt ttacgctgac acaaaccacg
     2641 cccaataccc tgcccgtgat ttctgatgtg cgtcaacaat ggctgccact aagctacacc
     2701 ggtgaccaga taagtg
//
LOCUS       DAOOOL010000242         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4634_length_2701_cov_164.547997, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000242 DAOOOL010000000
VERSION     DAOOOL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4634_length_2701_cov_164.547997"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2521)
                     /locus_tag="PKU82_12180"
     CDS             complement(<1..2521)
                     /locus_tag="PKU82_12180"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOZ83665.1"
                     /translation="MKQYTKFNNISGWAVFAIAAWVFLSTIEMTGSFWDCGEFIAAAY
                     KLQIGHPPGAPLFLMLSRIMTLFAGADVKDVPLMVNAFSAIMSAFAVLFTFWTITAIA
                     RKIVFVKNQEPTLAQILTVVASGVVGALAFTFTDSFWFSAVEGEVYATSQFFTCIVVW
                     AVFKWENVADEKHSIRWIILIAYLMGLSIGVHLLNLLCIPALALVYYFRKYPFSWMGV
                     AKTMGIALVILMVVQYGIIAGFVNIGAKIDLWFNQSGSSLWTGFVVYSLIILAALTYG
                     IYYSQKKQKALLNTILLSFAFLLLGYTSYAQIVVRSLANPPMDENNPENAFALLGYLN
                     REQYGDRPLGYGQYYDAKVVGQTEGAMSYTAIDGKYIPTGAKVIPEYDPARCTVFPRM
                     YSREPNHVSAYKEWSGIKGEQKPEFSNNLKYFFSYQMNHMFWRYFMWNFVGRQNDIQG
                     HGSILKGNWISGIPFIDSMFLGPQDKLPDSMKNNKARNTFYFLPLLLGLAGLVYHYKK
                     DMNNANVVMLLFIMTGLAIVIYLNQTPYQPRERDYAYAGAFYAFCIWIGLGVAAIADA
                     LKKRMPETTAAMLATLLCLVVPTVMAKEGWDDHNRSYRYTSRDFAYNYLNSCAPNAVI
                     FTNGDNDTFPLWYIQDVEGVRTDVRVINLSLLNTDWYIDQIKRKAYESDPVPFTLNTK
                     QYVQGTRDYIPFYDRSVQGYSDLKDIIEFITSDNPEAKVRSEGGDEFNYLPTKKFRIK
                     VDKQAVIKNNVVEAKDTSKIVDYIEFEISKNYIMKADLMILDLIAHNDWTRPIYFAVT
                     VGNDSYLNLENYFQTEGLAYRFVPIKAAADPSGQTGSVAAER"
BASE COUNT          819 a          578 c          526 g          778 t
ORIGIN      
        1 tgcgttctgc ggcaacactt cctgtctgtc cggatgggtc ggcagcagct ttaatcggaa
       61 caaaacgata agccaatcct tctgtctgaa aataattctc aagattaaga taagaatcat
      121 tgccaacggt aactgcaaaa taaataggac gtgtccagtc attgtgtgca ataagatcca
      181 atatcatcag gtcagctttc atgatatagt ttttgctgat ttcaaattca atataatcaa
      241 caatcttgct tgtatctttt gcctccacca cattgttctt aatgacagct tgtttgtcaa
      301 cttttattct gaactttttt gtaggcagat aattaaattc atcaccacct tcgctgcgca
      361 cttttgcttc ggggttatcg ctggtaataa attcaataat atctttcagg tcgctgtatc
      421 cctgcacact tctgtcgtag aatggaatat aatcacgtgt tccctgaaca tactgttttg
      481 tatttaatgt aaaaggaaca gggtctgatt cataagcctt acgttttatc tgatcaatat
      541 accagtctgt gttgaggaga ctcaggttta taacacgcac atcggtacgc acaccttcaa
      601 catcctgtat gtaccacaac gggaaggtat cgttatcgcc attggtaaaa attactgcat
      661 ttggagcaca ggagttcaga tagttgtatg caaaatcgcg tgaggtgtaa cggtaagagc
      721 ggttgtgatc atcccatcct tctttagcca taactgttgg cacaacaagg cagagcaacg
      781 tagccagcat tgccgcagtt gtttcaggca ttcgcttttt taaagcatcg gcaattgctg
      841 ccactcccaa acctatccaa atacaaaatg cataaaaggc tcctgcataa gcataatcac
      901 gttcgcgggg ctgataaggt gtctgattca ggtaaattac aattgcaagg cctgtcatga
      961 tgaaaagcaa catcacaaca ttggcattgt tcatgtcttt tttatagtga taaaccaatc
     1021 ctgcaagacc taacaacagt ggcaagaagt aaaatgtatt tcttgcctta ttatttttca
     1081 tactgtcggg tagtttgtcc tgcggaccta aaaacatact gtcaataaat ggtatgccgc
     1141 ttatccagtt tccttttaat atgctgccat gtccctgtat atcgttctga cggcctacaa
     1201 aattccacat aaaataacgc cagaacatgt ggttcatctg atagctgaaa aaatatttga
     1261 ggttgttgct gaactcaggc ttttgttcac cttttattcc tgaccattct ttgtatgccg
     1321 aaacatggtt aggctctcgg ctgtacattc ttggaaaaac ggtacaacgt gcaggatcgt
     1381 attcgggaat aaccttggct cctgtcggaa tatattttcc gtcaatagcg gtgtagctca
     1441 ttgcgccttc tgtctgacct acaactttgg catcgtaata ttgtccatag cctaaaggtc
     1501 tgtcgccata ctgctcgcgg ttgaggtagc ctaatagtgc aaatgcattc tccggattgt
     1561 tttcgtccat tggcgggttg gctaaggagc gcactacaat ctgtgcatag gaagtgtatc
     1621 ccaacaacag aaatgcaaag ctcagcaaaa tagtgttgag cagtgctttt tgttttttct
     1681 gagaatagta aataccataa gtcagtgcag ctaaaatgat gagagagtaa acaacaaatc
     1741 ctgtccataa agatgagcct gactgattga accataaatc aatttttgcg cctatgttta
     1801 caaaccctgc aataattcca tattgtacaa ccattaaaat taccaatgct atacccatag
     1861 tttttgcaac acccatccac gaaaacggat atttgcggaa gtaatatacc aatgccaaag
     1921 caggaataca aagtaagttc agcaagtgaa caccaataga caagcccatg aggtaggcaa
     1981 tgaggattat ccaacggatg ctgtgctttt cgtctgcaac attttcccat ttaaatacag
     2041 cccaaacaac aatacaggta aagaactgtg atgttgcata aacctcgcct tcaacggcag
     2101 aaaaccagaa tgaatctgtg aaagtaaatg ccagagcacc tacaacgcca ctggcaacta
     2161 cggttaaaat ctgtgctaat gtaggctcct gattttttac gaaaacaatt tttcttgcaa
     2221 tggcagtaat tgtccagaag gtaaacaata cggcaaatgc actcataata gcactgaatg
     2281 cgttaaccat tagcggcaca tcttttacat cggcacctgc aaaaagagtc ataatgcgtg
     2341 agagcatcag aaataatggc gctccgggag gatggcctat ctgtagtttg taggccgcag
     2401 caataaactc gccacagtcc caaaaactac ctgtcatttc aatggttgac agaaataccc
     2461 atgctgcaat ggcaaaaact gcccagccgg aaatgttgtt gaattttgta tattgtttca
     2521 tgtaaaacaa agaaggttac aagcgtgcga aaatagcaaa ttatgcttct gaatggcttt
     2581 taattttaaa taagctttag gtaccactat gaatctttaa gtggtagcaa gaaaaacatg
     2641 tcattgcttc accaccttaa cccgtgctgc gttatacccg tctaacacca acacagcaag
     2701 g
//
LOCUS       DAOOOL010000243         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4688_length_2677_cov_42.163616, whole genome shotgun sequence.
ACCESSION   DAOOOL010000243 DAOOOL010000000
VERSION     DAOOOL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_2677_cov_42.163616"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2677
                     /locus_tag="PKU82_12185"
     CDS             <1..>2677
                     /locus_tag="PKU82_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83666.1"
                     /translation="GNTTLLNDKINTASQALKESAAITEQYELKNNNLAGQIDRLQKN
                     LYALATSRTLSEWMAAAVEGTNKLITAIGKLPEFISKNSSAFFALAAAIAIYYANIIR
                     ATIATAANMVATVAWRGAAIAANAIMIALDVAIAAYSLTVGVLTGRITLAAAAQRIWN
                     AVMAINPVGLFIAGLAAAVIAIDKFTTSAKQAMQLEIFKADITKRLAAAQEKFNKTQE
                     EFGAQVQNINTLLPEQRKELERNIKLKIEDAKATLEQLTAEQKRIGELANKATIWQSA
                     MNFLSHPLNWKAAAEADREDGKENAKKEMAKYAPQIIDLNNKIKSVTESTGELVSVNN
                     AFANAMKINVRTIDQYNDKLNLLNKALNASVKGSAEYKKILAEIENTKKAFGSTDAVS
                     TGGEVKYTLKLTTEEAEKQMQEFKRKMQTLQEELSKLKTSAIADDVEREAVQMQQAYE
                     QSKRAALQKEEELLAEQIRIREENIADVSKAEAAGVIRKSEAVAKKLQIEQTYNDNAA
                     QIHETGLQLQKQLDEKYERDTAEFVKRTQDDLRKAEYQAHISNLEQWQQEKNLLIALD
                     YAKGTITHEQYEQQKADIDKRILELRLQYAKDYGRDLTAEEKAIVDAKIAQALREANA
                     REATLEKVKGYESNSFEGRMAALDAAEQQELDSVKGNQEAINAILQYYSEKRIKLRQE
                     EAVKWIGVAQQFFNAYSDMLQSSINEENNLENAQFNKEKALGNKKKEEYKRMLDSKLI
                     SQDDYNSKVEAIDKVLEEKQRRIKRDQWERQHDADILQAETNAILAIARAFVDYEWPY
                     SLIVAALVGATAYMKVSELESQTAPEFRYGKEAGEPIQKQKGGIVHGPTHEQGGIDLI
                     DSITGKKLGEMEGLEMIVGEKAVEANPEIAQLLMKS"
BASE COUNT          994 a          491 c          631 g          561 t
ORIGIN      
        1 ggcaatacaa cattgcttaa tgacaaaata aacacagcaa gccaggcact taaagagagt
       61 gctgccatta cagagcaata tgagttaaag aataataacc ttgccggaca gatagaccgt
      121 ttacagaaaa atctttatgc ccttgccacc agtagaacat tgagtgaatg gatggcagca
      181 gctgttgaag gcaccaataa acttatcaca gccataggta aactacctga gtttatttca
      241 aaaaacagta gtgccttttt tgcgcttgcg gcagctatag ccatatacta tgctaacatc
      301 attagagcaa cgattgccac agctgccaac atggttgcca cagtggcctg gcgtggtgca
      361 gcaatagcag cgaatgcaat aatgattgca ttggatgttg ccattgctgc ctattcattg
      421 acagtaggtg ttttaacagg aaggataaca ctggcagcag cagcacagcg aatatggaat
      481 gcagtcatgg caattaatcc ggttggttta tttattgcag gattagctgc ggctgtaatt
      541 gccattgata agtttaccac gagcgcgaaa caggcaatgc agttggaaat ttttaaagcc
      601 gatataacaa aacgtttagc ggcagcacag gaaaaattta acaaaacgca ggaagaattt
      661 ggcgcgcagg tgcaaaacat aaatacgctg ctgcccgaac agcgcaaaga actggaacga
      721 aacataaagc tgaaaataga agatgcaaaa gccacacttg agcaacttac ggcagagcag
      781 aaaagaatag gtgagttggc caataaggca acaatatggc aatcggcaat gaattttctt
      841 tcgcacccgc ttaactggaa agctgctgct gaggcagaca gagaagacgg taaggaaaac
      901 gcgaaaaaag aaatggcaaa atatgcaccc caaataattg atttgaataa caaaatcaaa
      961 agtgtaactg aatcaactgg tgaactcgtg agtgttaata atgcttttgc caacgccatg
     1021 aaaataaatg tccggaccat tgaccagtat aatgataaac tgaacctgct caacaaagca
     1081 ctaaatgcca gcgtgaaagg cagtgccgaa tacaagaaga ttttagctga gattgaaaat
     1141 actaaaaaag cctttggctc aaccgatgca gttagtaccg gtggcgaagt aaaatatacg
     1201 cttaaactaa caacagaaga agctgaaaag caaatgcagg agtttaagcg caaaatgcaa
     1261 acgctgcaag aagaattaag caaactaaaa acaagtgcta ttgctgatga tgtagagcgt
     1321 gaagcggtgc agatgcagca ggcttatgag cagagcaaac gggcggcatt acagaaagaa
     1381 gaagagctac ttgcagaaca aataagaatc agagaggaaa acattgcaga tgtaagcaaa
     1441 gcagaagcag ccggagtgat ccgtaagagt gaagcggtag ccaagaaatt acaaattgaa
     1501 caaacctata atgataacgc agcgcagata catgaaaccg gtctgcaact gcaaaagcag
     1561 cttgatgaaa aatatgagcg cgacacagcg gaatttgtta aacgtactca ggatgattta
     1621 cgcaaagcag agtatcaggc acacattagt aatcttgaac aatggcaaca agaaaaaaac
     1681 ctgcttattg cgctcgacta tgcaaaagga accatcacac atgagcagta cgaacaacag
     1741 aaggctgata ttgataaacg tattttagag ttaagactcc aatatgctaa agattacggg
     1801 cgcgatttaa cagcagaaga aaaagctatt gttgatgcta aaattgcaca agcattacgc
     1861 gaagccaatg ccagagaagc aacacttgaa aaggttaaag gctatgaatc caattcattt
     1921 gaagggcgca tggcagcact tgatgctgcg gagcagcagg aactagattc tgttaaaggc
     1981 aatcaggagg ctatcaatgc catactccaa tattatagtg aaaaacggat aaagctgaga
     2041 caagaagagg cggttaaatg gattggtgtt gcacaacagt tttttaatgc ttacagcgat
     2101 atgcttcaat cgagcattaa cgaagagaac aatctagaaa atgcgcagtt taataaggaa
     2161 aaagcactcg gcaacaagaa gaaagaagaa tacaagcgca tgcttgattc gaaattaatt
     2221 tcgcaggatg attacaacag caaggtagaa gccattgaca aggttcttga agaaaaacaa
     2281 agaagaataa agcgcgacca atgggagcga caacatgatg cagacatatt gcaggcagaa
     2341 accaatgcca tacttgccat tgccagagca tttgttgact atgagtggcc ttattcgctt
     2401 atcgttgcag cacttgttgg tgcaacggct tatatgaagg tatcggaact tgaaagtcag
     2461 acagcaccgg aattccgcta tggaaaagag gcaggagagc ccatacagaa acaaaaagga
     2521 ggcattgttc atggccctac acatgagcaa ggaggaattg atttgattga ttcaataacc
     2581 ggtaaaaagt tgggagaaat ggaaggattg gaaatgatag taggagaaaa ggcagtggaa
     2641 gcgaatccgg aaatagcaca actgctaatg aagagca
//
LOCUS       DAOOOL010000244         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4796_length_2628_cov_33.450836, whole genome shotgun sequence.
ACCESSION   DAOOOL010000244 DAOOOL010000000
VERSION     DAOOOL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_2628_cov_33.450836"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2534)
                     /locus_tag="PKU82_12190"
     CDS             complement(<1..2534)
                     /locus_tag="PKU82_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOZ83667.1"
                     /translation="MLRNVYLTCLLTLIFGSAAWAQSGSIKGRVLDKTTNEPLPFASV
                     IAVMNGQQMGGAQTDFDGNFTIKPLGAGTYSVKATFVGYTTTEVTGVLVSVDKITFAD
                     VKMGKGAVDITAVEVTAYKVPLIDKGNPAVQTTITQEEIKAIPTRDVRSVAATAAGVF
                     QRDEGDDLNVRGSRADATDYYIDGVKVRGSSQLPQSAIEQVTVITGGVPAQYGDATGG
                     IISITTRGPSKKFTGGIELATSELTDKFGYNLASGNISGPIYMKKDKDGNKTPVVGFF
                     LGVEGEWIKDPDPSAIGSKRVKGSVLTDLENNPLMPNMVNGTINGTRARSEFIHSSDI
                     ETMKYKPNTRSGEYKVSGNIDFQPANNINIKVGGSYDYHDYREYTYTYSLFNYKNYSR
                     HITNDYRGYLRFTQRFNTEGKDGAKSILKNVYYSLQFDYSKAFELVQSEQHKDRLFDY
                     GYWGRFNTLRRPVFAGNELLGYLPYLTVFTPSGNNPNTEAYTNQYLSFFDENYAAHVN
                     EYYETGNTSQFTNTAYYSNPFVIRAGGGLINGQRAGTVYSMWYNTGRTIDGYNYIDRD
                     QYRASAMFSADIKNHAISLGFEYEQRVDRQFNIAPSGLWTFMNNYANFYLNEANPEAG
                     SYVINDTIYHPNTYTNTTGQVGFYENVRKTLGLSNTDYVDVFNYDPSNFSLSMFSPDE
                     LGTSGLTLYYGYDYKGNRQKTLSPSATFDNFFKKDANGVYQRNIAAFQPIYLAGYIQD
                     NFSINDLIFNVGLRVDRFDANQRVMKDPYLLYQAYTAGELNFQRPDNIGDDYVVYVSD
                     FNVDKNTLTANDILGYRSGDQWYDKNGNALTDPRILERQSSSARVLP"
BASE COUNT          806 a          520 c          452 g          850 t
ORIGIN      
        1 ggcaatacac gggcagaaga actttgtctt tcaagaatac gtgggtcagt taatgcatta
       61 ccatttttat cataccactg gtctccgctt ctgtatccta atatatcgtt tgctgtcaaa
      121 gtgtttttat ctacattgaa atctgataca tagactacat aatcatcacc aatgttgtct
      181 ggacgctgaa aattcaattc accagctgta taagcctgat ataataagta aggatctttc
      241 ataacacgct gattggcatc aaaacgatct acacgtaaac caacattgaa gataagatca
      301 ttgatagaga agttatcctg aatataaccg gcaagataaa ttggctggaa ggccgcaata
      361 ttacgttgat aaacaccatt tgcatctttc ttaaagaaat tatcgaatgt tgcagaaggg
      421 cttagtgttt tttggcgatt tcctttgtag tcataaccat aatataatgt taatccactc
      481 gtacctaatt catccggtga aaacattgat aaactgaaat tagaaggatc atagttaaac
      541 acgtcaacat agtcggtatt tgacaaacct aatgtcttac gaacattctc ataaaatcca
      601 acctgaccgg ttgtgtttgt ataggtattt gggtgatata ttgtatcatt aattacataa
      661 cttcccgcct ctggattagc ttcgttcaaa tagaaattgg catagttgtt catgaaagtc
      721 cataatccgg aaggtgcaat attaaattga cggtcaacac gttgttcgta ctcaaaccct
      781 aatgaaattg catgattctt gatgtctgca gaaaacattg ctgatgctct gtactgatct
      841 ctgtcaatat agttataacc atcaattgta cgaccggtat tataccacat actataaaca
      901 gtaccggctc gttgtccatt aatcaaccca ccacctgctc tgatcacaaa aggattactg
      961 taatatgctg tgttggtgaa ctgacttgtg ttaccggttt cataatattc attaacatga
     1021 gctgcatagt tttcatcaaa gaatgaaagg tattgattgg tataagcttc tgtatttgga
     1081 ttgttaccgg atggtgtaaa tacagttaaa tatggtaaat aacccaataa ttcattacct
     1141 gcaaaaacgg gtctgcgcaa agtattaaag cgtccccaat aaccataatc aaataaacga
     1201 tctttatgtt gctcagattg tactaattca aatgctttac tgtaatcaaa ctgtaagcta
     1261 taataaacat ttttcaagat tgatttcgca ccatctttac cttctgtgtt aaaacgctga
     1321 gtgaagcgca aataaccacg gtagtcattg gttatatgtc ggctgtaatt cttatagtta
     1381 aataatgaat atgtataagt atattcacgg taatcgtgat agtcataaga accaccaact
     1441 ttaatattta tattatttgc cggttgaaaa tcaatatttc cactcacttt atattctcct
     1501 gaacgtgtat tgggtttgta tttcatggtt tcaatatcag aactatgtat aaattcagaa
     1561 cgtgcacggg ttccgtttat agtaccatta accatgttag gcatcaatgg attattctcc
     1621 aaatcagtca acacacttcc tttaacacgc ttgcttccaa ttgcagatgg atcagggtct
     1681 ttaatccact ctccttcaac accaaggaaa aaacctacaa cgggagtctt atttccatcc
     1741 ttatcttttt tcatatagat aggaccggaa atattaccac ttgccagatt atatccgaat
     1801 ttatcagtca actccgatgt tgctaattca attcctcctg taaatttttt agatggtcca
     1861 cgtgttgtga tgctgatgat accacctgta gcatcaccat actgtgcagg tacaccacct
     1921 gttattacag taacctgctc tattgcagat tgaggtagct gtgaacttcc acgaacttta
     1981 actccatcaa tataataatc cgttgcatca gcacgagaac ctctaacgtt caggtcgtca
     2041 ccttcatcac gttgaaatac gcctgctgct gttgcagcca cagaacgcac atcgcgtgtt
     2101 ggaattgcct ttatttcttc ctgagtaatg gttgtttgca ccgcaggatt acctttatca
     2161 atcaaaggaa ctttataagc agtaacttca acagccgtga tatcaactgc acctttaccc
     2221 attttaacat ctgcgaaggt tattttatct accgatacca gtaccccggt tacttctgta
     2281 gtagtataac caacaaaggt ggcttttaca gaataggtac cggcacccaa aggtttaatg
     2341 gtaaaatttc cgtcaaaatc agtttgcgca ccgcccatct gttgcccatt cattacagca
     2401 atgacacttg caaaaggtaa tggctcgttg gtggttttgt ctaatacacg tcctttaatg
     2461 gatcctgatt gcgcccatgc agcactaccg aaaatcaagg ttagtaagca tgttaagtaa
     2521 acgtttctaa gcataggttg acctatttaa atttctgatt attgtattat aaatgtgaag
     2581 attttgttaa aaatatttta ttagtaggtg aaagttttgg tgtaatta
//
LOCUS       DAOOOL010000245         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4806_length_2625_cov_59.064981, whole genome shotgun sequence.
ACCESSION   DAOOOL010000245 DAOOOL010000000
VERSION     DAOOOL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_2625_cov_59.064981"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PKU82_12195"
     CDS             <1..1335
                     /locus_tag="PKU82_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOZ83668.1"
                     /translation="HIGASPSAQSYLVVEKIIQAAKLTGAEAIHPGYGFLSENADAAQ
                     AIINAGLVFIGPSPQSMRTMGSKLGAKEAAKKFNVPLVPGTDYAISDIDKARQIAVDI
                     GFPVLIKASAGGGGKGMRIVESEKAFNESVKSAMSEAQSAFGDGSVFIEKYIASPRHI
                     EIQVMGDNHGNVVHLFERECSVQRRHQKVIEEAPSGVLDEKTRSAMGQCAVMVAKACD
                     YSGAGTVEFLLDEKNNFYFLEMNTRLQVEHPVTEMITGIDLVREQLNVAAGNKLSFSQ
                     DDLKIHGHAVEVRVYAEDPANNFLPSVGKLTTYVPPRGVGVRVDDGYEQGMEIPIYYD
                     PMIAKLVTYGRDRNEAIARMIRAIDEYKITGCETTLPFCRFVMEHKAFVTAKFDTHFV
                     KKYFTPVVLEKTIADEAEIASLAAQLWLDEKKPEGIQQQQEKSISYWRNNRS"
     gene            1356..2003
                     /locus_tag="PKU82_12200"
     CDS             1356..2003
                     /locus_tag="PKU82_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOZ83669.1"
                     /translation="MIKKILNCKIGNAFAKTSVMKFSVWLCFLLTAFIKFSIGQTPVG
                     SSTAIYDGDTIAYYTLNAVDIYGFIPANASDDVRKYMKLRRDVLKAYPYAKIASAELR
                     FIEESVSKMTSEKEKKKFIKEQEKIMKEQFEKDLKNLTYTQGKILIKLIDRETGHTSY
                     NLVKDLRGSLQAFFWQGIAKIFSANLKSEYDPDGADKMIEKVVQSIEKGELQVRN"
     gene            2066..>2625
                     /locus_tag="PKU82_12205"
     CDS             2066..>2625
                     /locus_tag="PKU82_12205"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOZ83670.1"
                     /translation="MALASKSNLQEGLTYDDVLLVPAFSEILPRDVDTSTSFSRNIKL
                     NVPIVSAAMDTVTESAMAIAIAQEGGIGVLHKNMSIEQQTNEVRKVKRSESGMIHDPI
                     TLHQNSTIHEALGIMREQRIGGIPIVNDSKKLVGILTNRDLRFETDESRKVKEVMTSG
                     QLITVTGVVSLQKAEVILRQYKIEKLP"
BASE COUNT          862 a          465 c          560 g          738 t
ORIGIN      
        1 cacataggtg cctcaccatc agcacaatct tacttagtag ttgaaaagat tattcaagct
       61 gcaaaactta caggagcaga agctattcac cccggatatg gattcttatc agaaaatgcc
      121 gatgctgccc aagctataat taatgccggt ttggttttca tcgggccttc accgcaatcc
      181 atgcggacta tgggtagtaa attaggggca aaagaagctg ctaagaagtt taatgtgcct
      241 ttagtacccg gaacagacta tgccattagt gatattgata aagccagaca aattgctgtt
      301 gatataggtt ttcctgtatt gattaaagcc agtgccggtg gtggtggaaa aggaatgcgt
      361 attgttgaga gtgaaaaagc ctttaatgaa tctgtaaaat cagccatgag cgaagcacaa
      421 tctgcctttg gtgacggtag tgtttttatc gaaaaatata ttgcttcacc ccgacatatt
      481 gaaattcagg taatgggcga taatcatgga aatgttgttc atctttttga aagagaatgt
      541 tcggttcagc gcaggcacca gaaagtaatt gaagaagcac catcaggagt tttagacgaa
      601 aaaacaagat cagccatggg ccaatgtgca gtaatggtgg ctaaagcatg tgactattca
      661 ggagcaggta cggtagagtt tttgcttgat gaaaaaaata acttctactt tcttgaaatg
      721 aatacacgct tgcaggtaga acatcccgta acagaaatga taacaggaat tgatttggtg
      781 agagagcaat tgaatgttgc tgccggaaat aaattaagtt tcagtcagga tgatttaaag
      841 atacatggtc atgccgttga agtcagggtt tatgcagaag atcctgccaa taactttctt
      901 cccagtgtag gtaaactaac tacctatgtt ccacctagag gggtaggtgt aagggttgat
      961 gatggttatg aacaaggaat ggaaattcca atttattacg acccgatgat tgccaagtta
     1021 gttacctatg ggcgcgacag gaatgaggca atagcaagaa tgattagagc aattgatgaa
     1081 tataaaatta caggctgtga aaccaccttg ccgttttgcc gctttgtgat ggagcataaa
     1141 gcttttgtta ctgcaaaatt tgatacgcat tttgtaaaga aatattttac accagttgta
     1201 cttgaaaaaa caatagcaga cgaagctgaa atagcttcct tggctgcaca actgtggctt
     1261 gatgaaaaga agcctgaggg tattcagcaa caacaggaaa aatctatttc ttactggcgc
     1321 aacaaccgat cctgaaccaa cgtttggtta aacaaatgat taagaaaatt ttgaattgta
     1381 aaatcggcaa tgcatttgct aaaacttctg tcatgaagtt ttcagtttgg ctttgcttcc
     1441 tgttgacggc ttttatcaaa ttcagcattg ggcaaacacc ggtgggttca tcaacagcaa
     1501 tttatgatgg cgatactatt gcctattaca ctttaaatgc agtagatatt tacggtttta
     1561 tccctgccaa tgcatctgat gatgtacgca aatacatgaa actgcgcaga gatgttttga
     1621 aagcatatcc ttatgctaaa atcgcttctg ctgaacttag atttattgaa gagtctgttt
     1681 ccaaaatgac ttcagaaaag gagaagaaaa aattcattaa agagcaagaa aaaatcatga
     1741 aagaacagtt tgaaaaagat ttaaaaaacc tgacttatac gcagggtaaa atccttatca
     1801 agttgattga tcgtgaaaca gggcacacct cttataatct cgttaaagat ttacgtggct
     1861 cacttcaggc atttttctgg cagggtatag ctaaaatttt cagcgcaaac ctcaagtctg
     1921 agtatgaccc tgatggcgca gataaaatga ttgagaaggt agttcagtct atagaaaaag
     1981 gcgagcttca ggttagaaat taacttttaa tttactccac tcaagcggtt ttattctgta
     2041 tctttgcccg gcaaaagcaa ataccatggc acttgcatca aaatcaaact tacaggaagg
     2101 attaacctac gatgacgttt tactcgttcc tgctttctcg gaaatactgc cccgcgatgt
     2161 tgatacttca acttctttct caagaaatat aaagctcaat gtacccattg tttcagcagc
     2221 aatggatacg gttaccgaat ctgcaatggc catcgcaatc gcacaggaag gcggcattgg
     2281 tgttctgcat aaaaacatgt ctattgaaca acaaaccaat gaagtaagga aagtaaaacg
     2341 ttctgaaagt ggtatgattc atgacccaat tactttacat cagaactcca ccatacacga
     2401 agctcttggc atcatgcgcg aacaacgtat tggaggtatt cctattgtaa atgacagtaa
     2461 gaagttggta ggcattttaa ctaacagaga tcttagattc gaaacagatg aaagccgtaa
     2521 ggttaaagaa gttatgactt caggacagtt gattactgta acaggagtgg tttctttaca
     2581 aaaagctgaa gtgattttgc gtcagtataa aatagaaaaa ttacc
//
LOCUS       DAOOOL010000246         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4819_length_2620_cov_32.407797, whole genome shotgun sequence.
ACCESSION   DAOOOL010000246 DAOOOL010000000
VERSION     DAOOOL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4819_length_2620_cov_32.407797"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKU82_12210"
     CDS             <1..294
                     /locus_tag="PKU82_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ83671.1"
                     /translation="VLVSIDPETDTPEHLKEFSIKNEMTGPQWTFLRSAENATKEFAN
                     VLSMKYKKISPIDFSHSNIISTFKPNGILVSQEEGDINVDKVVTTVNQTVRKK"
     gene            400..1062
                     /locus_tag="PKU82_12215"
     CDS             400..1062
                     /locus_tag="PKU82_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermine synthase"
                     /protein_id="HOZ83672.1"
                     /translation="MAKRYSVFQWLFSFITPVIIHKDQSEVNSILETTLENGKLVLNS
                     AHANYSYGSLFRVFIKALLRFNIDIKNKKKILVLGMGGGSVVKFLLGNNTYAQLDTVE
                     IDRKIISIAKEMFDLSPSDRLKIYAANAVDYLKDCTEEYDLILIDLFIDDAVPEFCFT
                     ESFWKSIQNICLPKCDIVFNASMKQNNLQHNLDFLGAIHLKENLTIEQNLFFLLQPAV
                     SK"
     gene            1131..2015
                     /locus_tag="PKU82_12220"
     CDS             1131..2015
                     /locus_tag="PKU82_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83673.1"
                     /translation="MKRLYTTIFLTLVAISLQAQNATPNAGFENWNQVGNRFDPADWN
                     NLNPSTGIIGVLTCARASGADAHSGSYAIKLTTKLIPGFNVIANGIASTGTLITTPPY
                     GVSGGIAFTGRPDSITGWYKYTPAGTDQGFVELQLLGTTNTDTIGYVRFETPNATVST
                     YTRFAAAVNYLSTATPSNTIWILSSSKGVGPIVNSQIFIDDIAMVFNPSSVNTIDTKN
                     EVSILGNVIKEKIRFNYKNTGVYQFKLIDTAGRIVTDTELNSQQSTVDVSALDGLYFY
                     IIENNSGIINNGKVIIRN"
     gene            2017..>2620
                     /locus_tag="PKU82_12225"
     CDS             2017..>2620
                     /locus_tag="PKU82_12225"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ83674.1"
                     /translation="MRYLNNAKKSFLLIIANVLFGISCYAQMGVLRGNVYLKQNGEPI
                     AGAGIFPVNDKSIGVASDADGSYEINLPVGKQTIVYTLTGLKNDTLTLDIKDGTESIR
                     NIYLTSNLKNLNVVVVSASKYEQRIEDITVSMEVISPKLIENRNTTNVTSVLEQTPGV
                     TILDQEPQIRGGSGFSFGVGSRVATLIDGLPIMTGDAGRTE"
BASE COUNT          898 a          448 c          457 g          817 t
ORIGIN      
        1 gtactagtat ctattgaccc tgaaacagac actccggaac atctcaaaga gttttcgatt
       61 aaaaacgaaa tgacagggcc acaatggaca ttcttacgct ctgctgaaaa cgcaacaaaa
      121 gaatttgcaa acgtactttc aatgaaatac aaaaagattt cacctattga tttttcacac
      181 tcaaacatta tcagcacctt taaaccaaat gggattttag taagtcagga agaaggtgat
      241 attaatgttg acaaggttgt tacaacagtc aatcaaactg taagaaagaa ataaaacaat
      301 taaaaaagag aacaatctca tttaacataa aactactttt taggcagtag ttaactccct
      361 ttgcttccat agaatattct acgtagttat ctttgcataa tggcaaaacg atattctgta
      421 tttcaatggt tgtttagttt tataactcct gttatcattc acaaagatca atcggaagta
      481 aatagtattt tagaaactac attggaaaat ggcaaactgg ttctgaattc tgctcatgcc
      541 aattacagct atggaagtct atttcgtgtt tttataaaag cattgcttcg ttttaatatt
      601 gacatcaaaa acaaaaagaa aatattagtt cttggtatgg gcggtggcag tgttgtaaag
      661 tttttattag gaaacaatac atatgcacaa cttgatactg ttgaaattga ccggaagata
      721 attagcattg caaaagaaat gtttgacctt tctccttctg atagattaaa aatttatgca
      781 gctaatgccg ttgactacct taaagattgt acagaggaat atgatttaat tttgatagac
      841 ctttttattg acgatgctgt tcctgagttt tgtttcacag aatctttttg gaaatcaatt
      901 caaaacattt gtctgcctaa atgtgacatt gtttttaatg ccagtatgaa gcaaaataac
      961 cttcaacaca atcttgattt tttaggcgca atacacttaa aagagaatct taccatagaa
     1021 caaaatctgt ttttcctgtt acaacctgct gtgagtaagt aatttactat cactataaaa
     1081 ttttaattca ttgcactata tttgtccggt taatgcaaac tccattacta atgaaaagac
     1141 tttacacaac tatattttta actttagtag ccattagcct tcaggcacaa aatgcaacgc
     1201 ctaatgcagg ttttgaaaac tggaatcagg ttggaaatcg ttttgaccct gccgattgga
     1261 ataatctgaa tccaagcacc ggaataatag gtgtgttaac ttgtgctcgt gcttcaggtg
     1321 cagatgcaca cagtggaagc tatgcaatta aattaactac taaattaatt cccggattta
     1381 atgttatagc aaatggtata gcaagtacag gcacattaat cacaacacca ccttatggcg
     1441 tatcaggagg cattgccttt accggtcgcc cggatagtat tacaggttgg tataaatata
     1501 cacctgccgg tacagatcag ggatttgttg aattgcaatt gttaggcact acaaacacag
     1561 acactattgg ctatgtaaga tttgaaacac ctaatgccac agtaagtaca tacacacgat
     1621 ttgctgctgc tgtaaattat ctttctacag caacaccatc aaacactata tggatacttt
     1681 cctcaagcaa aggagtcgga cctattgtca acagtcagat ttttattgat gatattgcaa
     1741 tggtttttaa cccttcttct gtaaatacaa ttgatacaaa aaacgaagtt tccattctgg
     1801 gcaatgtcat aaaagaaaaa atacgattta attataaaaa taccggtgtt tatcaattca
     1861 aattaattga tactgccgga cgaatagtaa cagatacaga acttaactca caacaaagta
     1921 ctgtagatgt ttctgctttg gatggtttgt atttttatat catcgaaaat aattcaggca
     1981 ttatcaataa cggaaaagta attatcagga attaatatgc gctatctgaa taacgcaaaa
     2041 aaatcatttc tattaattat tgcaaatgtc ttgtttggca tttcctgcta tgcacaaatg
     2101 ggtgtgttga gaggaaatgt ttacttaaaa caaaatggtg aacctattgc aggtgccggt
     2161 atttttccgg tcaatgataa atcaataggt gtggcatctg atgctgatgg aagttatgag
     2221 ataaatctcc ctgttggaaa acaaaccatc gtttatacat tgacaggatt aaaaaatgat
     2281 acccttacat tagatattaa agatggtaca gagtctatcc gcaatatcta tcttacatca
     2341 aaccttaaaa atctgaatgt agtagttgtt tctgcaagta agtacgaaca acgtattgaa
     2401 gatattacag taagcatgga agttatcagt ccaaaactta ttgaaaacag aaacacaacc
     2461 aatgttacta gtgtactcga acaaactccg ggtgttacaa ttttagatca ggagccacaa
     2521 attcgcggtg gtagtggttt tagttttggt gttggcagtc gagtagctac actcattgac
     2581 ggccttccca ttatgaccgg tgatgcagga cgtacagaat
//
LOCUS       DAOOOL010000247         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4828_length_2616_cov_33.980867, whole genome shotgun sequence.
ACCESSION   DAOOOL010000247 DAOOOL010000000
VERSION     DAOOOL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_2616_cov_33.980867"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PKU82_12230"
     CDS             complement(<1..1168)
                     /locus_tag="PKU82_12230"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOZ83675.1"
                     /translation="MIKNIKITTAFAILAITLSTVSCVKKDDFDNAPTTNVDPDIVTN
                     ATVSSVVTLANATGPVQITSDLTFSAIIVADDKSGNYYKEMIVQDTTAGISILIDQPS
                     YFTSLFIGRRVFVKAKGLYIQAVNGTPKIGVLNAGAVAEIPAAEIGNYIVGGKWGLTV
                     VPAKRKLNALTDADLNTLVQFDSVAFAAAEVGLPFGDAANKSTVNRNLKDCSGNSLIV
                     RTSGYANFATTLIPCKAGSVTAIYQKYNTTPQVFLRDMNDVPANIERCDGVVCTPVYV
                     SIDSIRTLFSSGATTVPGGFHIKATVISDYTTSALDTRNVIVQDATGGICLRYSTAPN
                     FSMNSQYDFNVGGLTLGEFNGWLQISNMAGGLSITGLAPITPTVIDLATATANFE"
     gene            complement(1195..>2616)
                     /locus_tag="PKU82_12235"
     CDS             complement(1195..>2616)
                     /locus_tag="PKU82_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOZ83676.1"
                     /translation="ALYLIENRVIQTNRLSFNTTFNTILNDNITTSTGLSYQKQTSRF
                     YKEAEDLLGAEFFVDIDKFADQDFQDEQLSQSDLNHPNRIVREGDRFGYDYEAHITHA
                     QAWTQAVAKYSNIDIFGAIQAAYTSVYREGNYKNGLFPNDSYGKSKTYVFPDVGVKAG
                     ATYKVNGRNYVFANAGYMTRAPFFNNMYLSPRSRNTTVGDLTSEKITSVEGGYLHIAP
                     KIKGKLKLYYTNFKDGIESRSYYLQGFGTNSYVNYTLSGIEKVHAGTEISVDANLGMG
                     LSAMAVASIGQYYYSSRPTATITVDNTNTVQSTGEVVYMKNLHVDNTPQNAFTVGLNY
                     RSKKFWYVGVNANCFSNSYVEASPARRTGDIIDGVDENSQAWEKILGQERYDAQMTLD
                     LSAGWSIKLNNHIKALKRNTFFLINLNINNLLDNKDIITNGYEQGRLDKSAVLSGRPD
                     VFASKYSYAFGRTYMISFILRMN"
BASE COUNT          808 a          515 c          446 g          847 t
ORIGIN      
        1 gttcgaagtt agcagttgca gttgctaaat caataacagt tggtgtgatt ggtgctaatc
       61 cagtaattga aagacctcct gccatattac taatttgcag ccatccatta aattctccaa
      121 gagtcaaacc acccacatta aaatcatatt gagaattcat tgaaaaattt ggcgcagtac
      181 tataacgcaa gcaaattcca ccggttgcat cctgtacgat aacatttctt gtatcaagtg
      241 cagaagtagt gtaatcagaa attacagttg ccttgatatg aaaaccaccg ggaactgttg
      301 ttgcgccact tgaaaataaa gttcttatac tgtctattga aacgtaaaca ggagtacaaa
      361 ccacaccatc gcaacgctca atatttgcag gcacatcatt catatcacga agaaatacct
      421 gtggtgtagt gttatatttc tggtaaatag cagttacgct tcctgccttg cagggaataa
      481 gtgttgtggc aaaatttgca tagccgctag ttctaacgat tagtgaattg cctgaacaat
      541 ctttcaaatt gcgatttaca gttgacttat ttgcagcatc gccaaatggc agaccaactt
      601 ctgctgcagc aaatgcaaca ctatcaaact gaacgagtgt atttaagtcg gcatctgtta
      661 atgcattcaa ctttcttttt gcaggaacaa cagttaatcc ccatttacca ccaacaatat
      721 aatttccgat ttctgcagca ggaatttctg caactgcacc ggcatttaac acaccaattt
      781 taggagtacc atttacagcc tgaatatata ggccttttgc ttttacaaat actcttcttc
      841 ctataaacaa agaagtaaag taagatggct gatcaattaa tatgctgata cctgcagttg
      901 tatcctgcac aatcatctct ttataataat taccggactt gtcatcagcc acaataatgg
      961 cagagaaagt taaatcagaa gttatctgaa ccggccctgt tgcattagct aaagttacca
     1021 cactcgatac tgttgcattg gtaacaatat ccggatcaac attagtggta ggtgcattgt
     1081 caaaatcatc ctttttaacg caggaaacag tacttaaagt aattgcaaga attgcaaatg
     1141 ctgttgtgat tttaatattc tttatcattg ttttattata tttctaatta gttattaatt
     1201 cattcttaaa ataaagctga tcatatatgt tctgccaaaa gcataactat attttgaagc
     1261 aaatacatca ggtcgtcctg aaagcacagc tgatttatcc aatctgcctt gctcatatcc
     1321 attggtgata atgtctttat tatcgagcag gttattgatg ttaaggttaa tcaggaagaa
     1381 agtatttctc tttaaagctt tgatatggtt gttcagttta attgaccaac ctgcactcag
     1441 gtcaagagtc atttgtgcat cgtagcgctc ctgtccaaga attttttccc atgcctgact
     1501 attttcatct actccgtcaa tgatatcacc tgtgcgtctt gcaggactag cttcaacata
     1561 agaattagag aagcaattgg cattaacacc aacataccag aattttttcg aacggtaatt
     1621 caatccaacc gtaaatgcat tttgaggtgt gttgtccaca tgcaggtttt tcatgtaaac
     1681 tacttcgcct gttgattgaa ctgtgtttgt attatcaaca gtaatagttg ctgttggacg
     1741 tgagctataa taatactgac caattgaagc tacagccatt gcagaaagtc ccattcccag
     1801 attagcatca acagaaattt ctgttcctgc atgtactttt tcaattcctg aaagtgtata
     1861 attcacataa ctgtttgtgc caaaaccttg cagataatat gagcggcttt caataccgtc
     1921 tttgaagttt gtatagtaca acttaagttt acctttaatc ttaggtgcga tatgtaaata
     1981 acctccttct acagatgtaa ttttttcact tgtaagatct ccgactgtcg tgtttctgga
     2041 acgtggtgac aaatacatat tattaaagaa tggcgctctt gtcatataac ctgcattagc
     2101 aaaaacatag ttgcgcccat taactttata ggtagcaccg gctttcacgc caacatcagg
     2161 aaacacataa gtttttgatt tgccataaga atcgtttgga aacaatccat ttttataatt
     2221 accttctctg taaacagagg tataagctgc ctgaatagca ccgaagatgt ctatattact
     2281 atacttggca acagcttgtg tccatgcctg tgcatgggta atgtgtgcct cgtagtcata
     2341 gccaaaacga tcaccttcac gaactattct gtttggatga ttcaagtcgc tctgacttaa
     2401 ttgttcatcc tgaaaatcct gatcggcaaa cttatcaatg tcaacaaaaa attctgcacc
     2461 taacaaatct tctgcttctt tataaaaacg tgaagtttgc ttctgataag ataagcctgt
     2521 actggttgta atattatcat ttaaaatagt attaaatgta gtattaaagc tcaatcggtt
     2581 tgtttgaatt acacgatttt ctattaaata taatgc
//
LOCUS       DAOOOL010000248         2604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4859_length_2604_cov_41.162024, whole genome shotgun sequence.
ACCESSION   DAOOOL010000248 DAOOOL010000000
VERSION     DAOOOL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4859_length_2604_cov_41.162024"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PKU82_12240"
     CDS             complement(<1..934)
                     /locus_tag="PKU82_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83677.1"
                     /translation="MKKLILLIAVCCIFTQTTIAQINLVPNPSFEDTVACPTFLTQID
                     RATGWMSFSYTPDYFNACASSNVPVSVSVPHNIWGDQTAHTGNAYAGFGAYSVGSLLS
                     IREYVCTQLIQPTVPLQKYYVSFYVSSSFAYTNGFNPGVACNNIGMKLTTHSYSPSTP
                     LIPDNLAQVVNHSIVNDTINWVKISGSFIADSAYDFLCIGNFFVDSLTNYVLIGANSG
                     DAYYYLDDVKLSTDSAFVNSIVETKDNGYIKIFPNPARDWIVVEGRNLKSIEVFDLCG
                     RLLIPKVLVSSFKTEVNISSFSSGIFLFKIYTSTG"
     gene            complement(1296..2180)
                     /locus_tag="PKU82_12245"
     CDS             complement(1296..2180)
                     /locus_tag="PKU82_12245"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOZ83678.1"
                     /translation="MIIDPTQIKTGEFHALMLGAVAPRPIAFASTVDKDGKPNLSPFS
                     FYNAFGSKPPILVFSPARRVRDNTIKHTLQNVFETREVVIGAVNYNIVHQASLASCEF
                     PEDVNEFEKAGFTAVKAEKVKPFLIKESPVNFECKVLDVIETGKLGGAANLIICEILL
                     MHIQDNVLNEQGKIDPDKIDLVARMGGDFYCRASGSAVFTVPKPNMKLGVGFDNIPEH
                     IKKSEVLTGNDLAQLANQETIPSTEEVAHFNQSDEMKKFNEALANDPLHAQLAKHLAA
                     RKLIHAGKVKEAWMLLLA"
     gene            complement(2220..>2604)
                     /locus_tag="PKU82_12250"
     CDS             complement(2220..>2604)
                     /locus_tag="PKU82_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83679.1"
                     /translation="EKKNDRPIVVDELNRIWALYADLQDNNGNTNLSKALLRTEPEVI
                     NETEIHVTVDNQVLRDNLLSLKSEFLEYFKKEMQNDFLTLEVRVEASEKVLDKPYTAR
                     EKFKKMAEKNPELENFRKQFDLNFD"
BASE COUNT          839 a          492 c          417 g          856 t
ORIGIN      
        1 taccggttga agtgtaaatc ttaaatagaa aaatgccaga actaaaagag gaaatattta
       61 cttctgtttt aaaggaagaa acaagcactt ttggaataag caatcttccg cataaatcaa
      121 acacttcaat tgatttaaga ttccttcctt ctacaactat ccagtctctt gcagggtttg
      181 gaaatatttt aatataacca ttgtcttttg tttcgacaat gctatttaca aaagcagaat
      241 cagtcgatag ttttacatca tctaaataat aataagcatc accagaattt gctcctatta
      301 aaacgtaatt agttaaagaa tcaacaaaga aatttccaat acataaaaaa tcataggcag
      361 aatctgcaat aaatgatccg gaaattttaa cccagttaat ggtgtcatta acaatactat
      421 gattcacaac ctgagctaaa ttatcaggaa ttaaaggggt tgatggagaa taggaatgtg
      481 ttgttaattt cattccaata ttattacaag ctacaccagg gttaaatcca ttagtataag
      541 caaaagaact agaaacataa aaagaaacat aatatttttg aagtggaact gtaggctgaa
      601 ttagttgtgt acaaacatat tctctaatgc ttaataacga gccaacacta taagccccga
      661 atccagcata agcatttcct gtatgtgcag tctgatcacc ccaaatatta tgaggcacag
      721 aaactgaaac tggtacatta gaagatgcac aagcattaaa ataatctggt gtataactaa
      781 aactcatcca cccagttgct ctgtctattt gagttaaaaa tgtaggacaa gctaccgtat
      841 cctcaaaact cggattgggt acaaggttaa tttgtgctat tgttgtttga gtgaaaatac
      901 aacagacggc aattaatagt atgagttttt tcattttact tttcttttta ttcttaaatc
      961 caaaacaggt gcagcacttt gccttttacc caattgcaga cttttgaata gtttcgttaa
     1021 ttagtgattt gtaaaactaa aaatgtaaaa aaaattctgt tgtggctgcg gttaagaaaa
     1081 gcgcagcata ctcccggata aaagcaaaca aagtctaatt tctaacatgg cattgttatt
     1141 tagtgctgta aaagtaagtg tttagttttt aaaaacgaat gtttttgtgg ctatttattt
     1201 gaacactagc ttactttaaa acacagcaac ttcagattca agaaatttta aactttgcta
     1261 ttaaagtgcc tctatcactt aattgctgcg ttttattatg ccagcaacaa catccaagct
     1321 tctttaactt ttcctgcatg tatcaatttg cgtgcagcaa gatgtttggc caactgtgca
     1381 tgcaatgggt cattggcaag cgcttcgtta aattttttca tttcatcaga ttgattaaaa
     1441 tgcgctacct cttctgtaga aggaatagtt tcctgattgg ctaactgcgc caagtcgtta
     1501 cccgttagca cttcactttt cttaatatgt tcaggaatat tatcaaaacc tactcccagt
     1561 ttcatattgg gcttaggcac ggtaaaaaca gcgctaccgg atgcacggca ataaaagtcg
     1621 ccccccatgc gtgctaccag atcaatttta tcaggatcaa ttttaccttg ctcattcaac
     1681 acattatcct gtatgtgcat gagcagtatt tcgcaaatga ttaaattagc agcaccacct
     1741 aatttacctg tttcaataac atctaaaact ttacactcaa aatttacagg cgactctttt
     1801 atcagaaaag gttttacctt ttctgcttta acagcagtga atcctgcttt ttcaaactca
     1861 tttacatctt ccggaaattc gcaacttgct aatgatgcct gatggacaat gttataattt
     1921 acagcaccaa tgaccacttc gcgtgtttca aaaacgtttt gcaaagtgtg ttttatggta
     1981 ttgtcgcgca ctcttcgtgc aggactgaaa acaagtattg gcggcttact gccaaaggca
     2041 ttataaaaac taaaagggct cagatttggc tttccatctt tatcaaccgt actggcaaag
     2101 gcaatgggac gtggtgcaac agcaccaagc atcaatgcat gaaactcacc tgtttttatc
     2161 tgcgtgggat ctattatcat tatttacttt tctaaaacaa ctttgaaatt tatttttgac
     2221 taatcgaaat taaggtcaaa ctgctttctg aaattttcaa gctcaggatt tttttcagcc
     2281 atcttcttaa atttctctct tgcagtatag ggtttatcaa gaactttctc tgatgcttca
     2341 acacgaactt ccaaagtcag aaaatcgttc tgcatttctt ttttgaaata ttcaagaaat
     2401 tcacttttca acgacaataa attatcccgt agcacttggt tgtcaacagt aacatgtatt
     2461 tctgtttcat taatcacttc aggctctgtt ctgagcaatg cttttgaaag attggtgttg
     2521 ccattattgt cttgcaaatc tgcatataaa gcccatatcc gatttaactc atcaacaact
     2581 atcggtctgt catttttttt ttca
//
LOCUS       DAOOOL010000249         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4906_length_2586_cov_41.566179, whole genome shotgun sequence.
ACCESSION   DAOOOL010000249 DAOOOL010000000
VERSION     DAOOOL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_2586_cov_41.566179"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="sucC"
                     /locus_tag="PKU82_12255"
     CDS             complement(<1..736)
                     /gene="sucC"
                     /locus_tag="PKU82_12255"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679599.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HOZ83680.1"
                     /translation="MNIHEYQGKSILKSFGVAIQEGIVAETADQAVVAAKELQNQTGT
                     NFWVVKAQIHAGGRGKGGGVKLAKSIDEVKEKANAIIGMQLVTPQTGPQGKKVHKVLI
                     AQDVYYPGETKTAEFYMSVLLNRQAGKNIIMYSTEGGMDIEEVAAKTPEKIFKEEIDP
                     AVGLQPFQARKIAFNLGVSGNAYKEMVRFVTALYKAYESCDASLFEINPVLKTSDNKI
                     IAVDAKVNLDENALFRHADYAAMRDKS"
     gene            1094..>2586
                     /locus_tag="PKU82_12260"
     CDS             1094..>2586
                     /locus_tag="PKU82_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83681.1"
                     /translation="MKRLFTTLSLGLATMVGFAQSNCYFSEDMETIDSVTSTPANAWV
                     QNTRLSVSGVACDSAQVLNGTDAILETDPIDLTGFFFVSVTFQHICRIDFFDAANVDV
                     SIDGGVTWTTLGPAEFDSTLVDYPAFFGQAYFSSATTLDWDPGVPGTMASNSMWHLAR
                     FNLSQWGGVSDFRMRFRLSDVNGGGAGGYPGWFIDDICIQAAVCELNPPTIAFNSGYP
                     QGTIYNLGPYNVQSHITDASGIMSATLTYTINNNPPVSVLMTDLGNGDYLGQIPAVND
                     GDSICYSITAVDNSGCANSAVFPTSGCITFYASQGITFPFCDNFDLQNLWTDSLGTAG
                     STPWQRGQSTVWPGSAHSAPNIWEVGLDQQYVGSCLSYLYSPTFSFLGVFNAQIELWI
                     NSNCENFWDGVRLEYTTDNGATWNVLGTNNTTFPTGQNWYNDGQLNSSQTYAWTGTSQ
                     VIAGTPGWFKAMHTLSMLDNQTSVMFRFVFTSDASVQADGFAIDDVC"
BASE COUNT          744 a          531 c          485 g          826 t
ORIGIN      
        1 ctgacttatc gcgcatggca gcataatctg catgacggaa caaagcattt tcatcgaggt
       61 taactttggc gtcaacagct ataattttat tgtccgatgt ttttaataca ggattaattt
      121 caaacaacga tgcatcacag ctttcatacg ctttatataa tgcagtaaca aaacgcacca
      181 tctctttata agcattacca cttacaccaa ggttgaatgc aatttttctt gcctgaaaag
      241 gctgaagtcc tactgcagga tcaatttctt ctttgaatat tttctcaggt gtttttgctg
      301 ccacctcttc aatatccatc cctccttcag tggagtacat gattatgttt ttacctgcct
      361 gacgatttag taaaacactc atataaaact ctgcggtttt tgtttcaccg ggataataaa
      421 catcctgtgc aattagtacc ttatgcactt ttttaccttg cggaccggtt tgtggtgtca
      481 ccaactgcat gccgataatt gcattggctt tttcttttac ttcatctatt gacttggcta
      541 gctttacacc tccaccttta ccacgaccac ctgcatgtat ctgtgcttta actacccaaa
      601 agtttgttcc tgtttgattt tgaagttctt tagctgctac tactgcctga tcggctgttt
      661 ctgctacaat tccttcctga atggctacac caaaagattt taaaatggat ttgccctgat
      721 attcgtgaat gttcattatg aaaaattttc acaaaaataa acttaaacat caaaatgatg
      781 ccttgttgtt aaataaaaag atttgaactt atgaaatcag ccgttatatt ggcttacaaa
      841 aaaatagata aaaatcgcaa tcaggattat gcttaacccc aattcctgaa gaaaactcaa
      901 atctttccag ataaaattat catctaatcg cttcatctcc caatgaattg tggtgtaaag
      961 aaacaaagtt ttatctattt tgcaaaatat tttaacaatt taaaaaaaat cctaaaagtc
     1021 aatttgcatg tcagagtatt tttatacttt tgaaacttgt aaaatcacaa atattcctta
     1081 aaatcaaaaa gtaatgaaaa gactgtttac tacactaagt cttggattag caacgatggt
     1141 aggctttgcc caatcgaatt gttatttctc agaagacatg gaaacaattg actcagttac
     1201 atcaactccg gccaatgcct gggttcaaaa cacacgcctt agcgtgtcgg gtgtagcctg
     1261 cgactcagca caagtgctca atggcactga tgctattctt gaaaccgatc caatagattt
     1321 gacaggtttc ttctttgtaa gcgtaacctt ccagcatatt tgccggattg acttttttga
     1381 tgcagccaac gtggatgtgt caattgacgg aggtgtgaca tggacaactt taggtccagc
     1441 agaatttgat tcaacattag ttgattaccc tgcgtttttt ggccaagcgt acttttcttc
     1501 tgcaacaact ttggactggg atccgggagt acccggaaca atggcctcaa acagtatgtg
     1561 gcacttagca cgctttaacc tgtcacaatg gggtggcgta tctgacttca gaatgcgttt
     1621 ccgtctatca gacgtgaatg gtggcggtgc aggcggttat cccggttggt ttattgatga
     1681 catctgtatt caggctgctg tttgcgagct aaaccctcca acaatagcgt ttaacagtgg
     1741 ttatccacag ggaaccattt ataaccttgg accttataat gtacagtcac atattactga
     1801 tgcttcaggc ataatgagtg ctactttaac ttatacaata aataataacc ctcctgtaag
     1861 tgtactcatg acagaccttg gtaatggcga ctacttagga caaattcctg ctgtaaatga
     1921 tggtgattcc atttgctact caatcacagc agtggataac tcaggctgtg ccaactctgc
     1981 agttttccct acatcaggat gtataacctt ctatgcatcg caaggaatta ccttcccgtt
     2041 ttgtgacaac tttgatttgc aaaatttatg gacggactca cttggcactg caggttcaac
     2101 accatggcaa agagggcaat caaccgtttg gcctggctct gcacattctg cacccaatat
     2161 atgggaggtt ggtttagatc aacaatatgt tggaagttgt ctttcttatt tatattcacc
     2221 tacattctca ttcttagggg tatttaatgc acagattgag ttatggataa attcaaattg
     2281 tgaaaatttc tgggacggtg ttcggttaga atatactaca gataatggcg ctacctggaa
     2341 tgtacttggg actaataaca caactttccc tacagggcaa aactggtata atgatggtca
     2401 attaaactcg agtcagacgt atgcatggac aggcacaagt caggtcatag caggtacacc
     2461 gggctggttt aaagccatgc atactttgag tatgttagat aaccaaacca gtgtgatgtt
     2521 ccgttttgta tttacctctg atgcatcagt acaagcagat ggttttgcaa ttgatgacgt
     2581 ttgtat
//
LOCUS       DAOOOL010000250         2581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_4925_length_2581_cov_20.020982, whole genome shotgun sequence.
ACCESSION   DAOOOL010000250 DAOOOL010000000
VERSION     DAOOOL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4925_length_2581_cov_20.020982"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKU82_12265"
     CDS             complement(<1..378)
                     /locus_tag="PKU82_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83682.1"
                     /translation="MSKRILVLYYSQTGQLKQIADNFCLPFVNSGCEVEFIEIVPMEK
                     FKFPWNGERFFDAMPECVLDIPVPIKKVELSKNRYDLIIIAWQPWFLSPSLPIISALK
                     DPLIKSVISQTPVITICGARNMWI"
     gene            complement(371..847)
                     /locus_tag="PKU82_12270"
     CDS             complement(371..847)
                     /locus_tag="PKU82_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83683.1"
                     /translation="MRVILSCLLFIIISAKPDEGMKLFIKVVENLSSTDTVAINKLIE
                     EVRFSKTNLAQACQGALLMKKADLVKRPAEKLSLFKEGRRLLEVEITKDSTYVNFRIL
                     RLIIQENCPAFLKYKSNIDDDANIVQGSFSKFSNQLQDVLLNYAKNSKHLKLKDRE"
     gene            1039..2367
                     /locus_tag="PKU82_12275"
     CDS             1039..2367
                     /locus_tag="PKU82_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ83684.1"
                     /translation="MRKILVLGAGKSSWHLINYLLSNAQEQNWHVTVADASVELAKSK
                     TQNHSASNAIAFNINDESQRKSEISKADIVISLLPPSLHAIAAVECLSEGKNLVTASY
                     VSEQMNALNNDVKNKNLIFLNECGLDPGIDHMSAMQIIHVLQAKGAEIISFKSYTGGL
                     ISPGSNNNPWGYKFTWNPKNVILAGQGTALYRQNRNNKFTPYHRLFSQIEKINISGTG
                     QFEGYANRNSNAYIETYNLQNISTLLRGTLRYAGYCSAWNLFVHLGITDDTYIINTSS
                     LTYHDWISSYLPDNEKSLIQNLQSQFSYLNLSTADWERIKWSGILDKTPIPLSSGTPA
                     EILLNLLESKWKMELGDLDLVVMQHIFEYILNGKTQTHVSSLSVKGENETATAMSKTV
                     GLPMAIAAKLILNGKVKTKGVAIPVVAEIYEPLLHELAQMGIVFIEEHIY"
BASE COUNT          872 a          499 c          378 g          832 t
ORIGIN      
        1 aatccacata ttgcgtgcac cacaaattgt aataacgggt gtctggctaa tcacagattt
       61 tatcagggga tctttcaaag ctgaaattat aggtaaggaa ggtgataaaa accagggttg
      121 ccaggcaata ataattaagt cgtagcgatt ttttgacaac tctacttttt ttattggtac
      181 aggaatatcc aaaacacatt caggcattgc atcaaaaaat cgctcaccat tccacggaaa
      241 cttaaatttt tccattggaa caatttcaat aaattctact tcacatcctg aattaacaaa
      301 tggcaaacaa aagttgtccg ctatttgctt caactgacct gtttgcgaat aatataaaac
      361 caatattcgc ttactcacgg tctttaagtt ttaaatgttt ggaattttta gcatagttta
      421 ataacacatc ttgcagttga tttgaaaatt tgctaaaact cccctgcaca atgttggcat
      481 catcatctat attactcttg tattttaaaa atgccgggca attttcctga attatcaatc
      541 gcaatattct aaaatttaca tacgtactat cttttgtaat ctccacctcc agcaatctcc
      601 ttccttcttt aaataaactt aacttctctg caggccgttt caccaaatct gcctttttca
      661 tcagcaatgc tccttggcat gcttgcgcca agttggtttt agaaaatcta acttcttcaa
      721 tcagtttgtt tattgcaaca gtatctgtag atgataaatt ctccacaacc ttaataaata
      781 acttcatgcc ctcatcaggc tttgcactaa tgatgataaa caaaagacaa cttaatatta
      841 ctctcatcta agattatgaa aaaattcatc tgcaagaaac agcaaaatat tttttgcata
      901 aattatttca cagattaacc gctgtaaaag tatgtttttt ttagcgcata actaagactc
      961 taaacaacct tattttggct gcaacaaaat acattcaaaa attcaatttg tacttttgcc
     1021 catatttaaa tttttactat gcgcaaaata cttgttttag gagccgggaa atcctcttgg
     1081 catcttatta actatttact ttcaaatgct caggaacaaa actggcatgt tactgttgcc
     1141 gatgcctcag tcgaattagc aaaatccaaa acacaaaacc attcagcttc aaatgccatc
     1201 gcttttaata taaatgacga atcacagcgg aaatcagaaa tctctaaggc tgacattgta
     1261 atttctttgc tcccaccttc acttcatgct atcgcagccg tagagtgtct atcagaaggt
     1321 aaaaatttag ttacagcatc gtatgtttcc gaacagatga atgcattaaa caatgatgta
     1381 aaaaacaaaa atcttatttt tttaaatgag tgtggacttg accccggaat agatcacatg
     1441 tcagcaatgc aaataataca tgttttgcaa gcaaaaggcg ctgagattat ctcgtttaaa
     1501 tcttacacag gtggattaat atcacctggg tcaaataaca acccatgggg atataaattt
     1561 acatggaatc caaagaatgt tattcttgcc ggacaaggca cagcgcttta cagacaaaac
     1621 cgaaacaata aattcacacc ataccatcgt ttgttttcac aaatagaaaa aataaatatt
     1681 tccggtacag gtcagtttga aggttatgct aatagaaatt caaatgccta tattgaaact
     1741 tataatctac aaaatatttc aaccctactg cgtggaacat taagatatgc cggctattgt
     1801 tcagcttgga atctctttgt tcatctcggt attactgatg acacatatat tatcaataca
     1861 tcttccctga cctaccacga ttggatttca tcctacttac ctgacaacga aaaatcctta
     1921 atccaaaatc tacaatcaca attttcatat ttaaatcttt caaccgcaga ttgggaaaga
     1981 ataaaatggt caggtatttt agacaaaaca cctattccgt taagcagtgg aacaccggca
     2041 gagattttat taaatttatt agaatcaaaa tggaaaatgg aacttggtga tttagacctt
     2101 gtagtaatgc aacatatttt tgaatatatc ctaaatggga aaactcaaac acatgtttcg
     2161 tcactaagcg taaaaggcga aaacgagaca gcaactgcta tgtctaaaac tgttggatta
     2221 ccaatggcaa tagcagcaaa actaattctt aatggtaaag tcaaaaccaa gggagtggct
     2281 atacctgtag ttgcagaaat ttatgagcct ttgctacatg aacttgctca aatgggtata
     2341 gtttttatcg aagaacatat atactgaaga aatttatcgc taacctttca aattacacac
     2401 taatgatata gtgccctatt aaatttcctt gattccatca cttttccacc ttcactttta
     2461 cttattctga atttcaacat catttttcga ttatacttcc ttccacttgt ttggttatta
     2521 attggattta gactattgaa tggtttttaa ttgcatggca atatttatat caacatagtg
     2581 t
//
LOCUS       DAOOOL010000251         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5024_length_2553_cov_206.194556, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000251 DAOOOL010000000
VERSION     DAOOOL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5024_length_2553_cov_206.194556"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKU82_12280"
     CDS             complement(<1..720)
                     /locus_tag="PKU82_12280"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M1"
                     /protein_id="HOZ83685.1"
                     /translation="MMQKLLFFFILSPFLSFSQDNVHIHCKRIISDNSKLHSLNTVST
                     TGSDFDVKYNRFNWTINPDSVYISGNVFTLFEVKAPISKLEFDFSSALTIDSILYQST
                     LLPYTSNSDIITILLPGIISTGQLDSIEIFYHGVPPTSGFGSFNQSTHNGVPVLWTLS
                     EPFGASDWWPCKNSLTDKVDSIDVFVTTDTAYKVASNGKLISEIASGNQKITHWKSNY
                     PTASYLIAIAVTNYAAYSDYVP"
     gene            complement(717..>1076)
                     /locus_tag="PKU82_12285"
     CDS             complement(717..>1076)
                     /locus_tag="PKU82_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOZ83686.1"
                     /translation="FEHYKSNPDVMFLSYSVNPEYDTPAVLKEYADKHHADAKKWHFV
                     TGNKQKIYDLARISYLVTATKGDGGPDDFVHTQNFALIDKEKRIRGYYDGTDSTDVNR
                     LITEIDLLLREYKYADK"
     assembly_gap    1077..1176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1177..1388)
                     /locus_tag="PKU82_12290"
     CDS             complement(<1177..1388)
                     /locus_tag="PKU82_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOZ83687.1"
                     /translation="MRKVIVALLILFTACQPKSSEPEQKTAPALEEKEFPAKPDIALA
                     SDIDPVCKMSVKEEFSDTAMYDGKIYG"
     gene            1486..1986
                     /locus_tag="PKU82_12295"
     CDS             1486..1986
                     /locus_tag="PKU82_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOZ83688.1"
                     /translation="MKKQVYTALTILMVFGVIKQTNAQEKVSIYHPEKNAFEQIKQAE
                     ITAQKEGKHIFLMIGGNWCRWCKMFDTFSKTDLSVDSAFKANYVVEHINYSKENKNDS
                     LMQQLEFPQRFGFPVFVILDAHGKRLHTQSTGYLEKGEGYNKESVIEFLSQWSPAALN
                     PANYKK"
     gene            complement(1996..>2553)
                     /locus_tag="PKU82_12300"
     CDS             complement(1996..>2553)
                     /locus_tag="PKU82_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83689.1"
                     /translation="LFTQIWKTECGGFKNESQNYSQIALANIVIFERFLQSNKDSLWL
                     VQGEKMMADYKKSKTPGIAGINKLKQSLELYSDDLMDGTIPADFFISRMTSIQKELAA
                     YKVKKTNEEETNLSMTQYLALGKQLIRKRQLDDAIKILKLNIEHYPNCVTAYDSLSRI
                     YKIQGNKQMALFYNQKGASIKNPAY"
BASE COUNT          782 a          404 c          438 g          829 t
ORIGIN      
        1 tggcacatag tcggaatatg cagcataatt ggtaactgca attgcaataa gataggatgc
       61 agtagggtag ttgcttttcc aatgcgttat tttttgattg cctgaagcaa tttcagaaat
      121 taattttcca ttggatgcta ctttgtaggc agtatctgta gttacaaaaa catctatact
      181 atccacttta tctgtaagtg agtttttaca tggccaccag tcgcttgcgc caaaaggttc
      241 tgaaagcgtc cacaagacag ggacaccatt gtgagtactc tgattaaaag acccaaaacc
      301 tgaagtaggt ggtacaccat gataaaaaat ttcaatacta tctaattgcc ctgttgagat
      361 tattccggga agtaaaatgg ttataatgtc actgttacta gtatagggta ataatgttga
      421 ttgatataaa atactatcaa ttgtaagtgc agatgaaaaa tcaaattcta atttggatat
      481 tggtgctttt acttcaaaca atgtaaatac atttccggaa atatatactg aatcaggatt
      541 tatcgtccag ttaaaacgat tgtatttcac atcaaaatca ctacctgttg tgctaacggt
      601 gtttaagcta tgaagtttcg aattatctga aataatacgt ttgcaatgta tgtgtacatt
      661 atcctgactg aaacttaaaa atggagatag aataaaaaag aagagtagtt tttgcatcat
      721 ttatcggcat acttgtattc acgcagtaac aaatcaattt cagttatcaa tctgttgaca
      781 tcagtagagt cagtgccgtc atagtaacct ctgattcttt tttctttgtc tatcaacgca
      841 aagttttgtg tgtgcacaaa gtcatccgga ccgccatcac cttttgttgc agttactaaa
      901 taggaaatgc gggcaaggtc atatatcttt tgtttgttgc cggtcacaaa atgccatttc
      961 ttggcatctg catgatgctt atctgcatat tcttttaaaa cagcaggagt atcatattca
     1021 ggatttactg aatacgataa aaacataaca tcaggatttg atttgtaatg ttcaaannnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccat aaattttacc atcatacatt
     1201 gctgtatcag aaaattcttc tttaactgac attttacaaa ccggatcaat gtccgatgct
     1261 aatgcaatgt ccggttttgc aggaaattct ttttcttcaa gtgccggtgc agttttttgt
     1321 tcaggctctg aagacttagg ttggcatgcc gtaaaaagaa ttaatagtgc tacaataact
     1381 tttctcattt ttttgtttat ttgaacttta caaagttatt aaatacaacc tttatcagat
     1441 gaaggattca ggtattattt taaacgaatc aattgaaaat ttagtatgaa aaaacaagtt
     1501 tacactgcgc tgacgatact catggttttt ggagtgatta agcagactaa tgcacaagaa
     1561 aaggtttcaa tttaccatcc tgaaaagaat gcatttgaac agataaagca agcagaaatt
     1621 actgcacaga aagaaggaaa acatattttt ctgatgattg gaggtaattg gtgtcggtgg
     1681 tgcaaaatgt ttgatacttt cagtaagaca gatttatcag ttgattcagc atttaaagct
     1741 aactatgttg tggaacatat taattacagt aaggagaata aaaatgattc actgatgcaa
     1801 caacttgaat ttccgcagcg ctttgggttt cctgtttttg taattcttga tgctcatggg
     1861 aagagattgc atacacaatc taccggctat ctcgaaaaag gagagggata taataaagaa
     1921 agtgtaattg agtttttaag tcaatggtca cccgctgcat tgaatccagc aaactataag
     1981 aaatagcttg aaaaattaat aagccggatt ttttatagaa gcaccttttt gattgtaaaa
     2041 taaggccatc tgtttattac cctgaatttt ataaattcgg ctcaatgagt cgtatgcagt
     2101 tacacagtta gggtagtgct ctatgtttaa tttcagaatc ttaattgcat catccaactg
     2161 acgtttacga atgagttgct ttcctaatgc cagatattgt gtcatactca gattagtttc
     2221 ctcttcattc gtttttttaa ctttatatgc agcaagttct ttctgaatac ttgtcatacg
     2281 ggaaataaag aaatctgccg gtattgttcc atccattaaa tcatcactat agagttcaag
     2341 actttgctta agtttgttta ttcctgcgat gcccggagtt ttgctttttt tataatcagc
     2401 catcattttt tctccttgta ctaaccaaag tgaatcttta tttgactgaa gaaaacgctc
     2461 aaaaatcaca atgttggcaa gcgcaatctg agaatagttt tgcgattcat ttttaaatcc
     2521 accacattct gttttccaaa tttgagtaaa caa
//
LOCUS       DAOOOL010000252         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5057_length_2543_cov_18.953376, whole genome shotgun sequence.
ACCESSION   DAOOOL010000252 DAOOOL010000000
VERSION     DAOOOL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_2543_cov_18.953376"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKU82_12305"
     CDS             <1..1205
                     /locus_tag="PKU82_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83690.1"
                     /translation="DTLNLTQGRLQLNSVAPTAIRSLSLMSNTGSTTALTRNTGVLIA
                     ESNPPNYGNFRWKIGTPAAYPTTFTFPFANAAGTYIPVDYKVNNGDADVTLTTYTTIP
                     ANTPIPTGVSDIYMNTWFAPPFTDNSPYMVDRFWQINDAGTVSSRDLTLNWTPTENAS
                     AGSSPYRAQVYSSSTNWGYPFIIGQTNPTATSVSIPAATFTNTWAVVRELQPLPVELL
                     SFEAKPYKDQEVVCNWTTATELNNDFFTVERSSDGRNFEKVGTVKGAGNSTQQHDYKF
                     IDTKPYRGVSFYRLKQTDFNGKSESFEKVAVNLSLPATPISVYPVPANEYVVFSVDRN
                     QNLENAVLYVYDFTGRALIQKKVAELKGTGTNIFILPVNTLINGLYHFDLKVEGASLG
                     NGKFIVEK"
     gene            1234..1893
                     /locus_tag="PKU82_12310"
     CDS             1234..1893
                     /locus_tag="PKU82_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOZ83691.1"
                     /translation="MISKQTLSFLRALAVNNNREWFAANHHKYEGAKEDFNNYLKQTL
                     TELAKYDVLLKSLEPKDCVFRIYRDIRFSPDKTPYKTNLGAAINAGGKRVETAGAYLH
                     IQLGNKSFFAGGRWMPDRETLRKIRQEIDYNTGEFKAILNDKNFKKYFGTLEDIALKK
                     APKDYPKDHPEIALLRYTSYIASMPLADSLITSDKGIKEIGKAYKALMPLLHFLNRAL
                     D"
     gene            complement(1890..>2543)
                     /locus_tag="PKU82_12315"
     CDS             complement(1890..>2543)
                     /locus_tag="PKU82_12315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ83692.1"
                     /translation="TFTLSMLHNKKVHHVLGKSQTQQIASPYKDKIYLYFIVAAFIYA
                     LCFFQFFITMPLFYKDIHHLSERQIGLLMMFNGLIVAVVEMILVYRIEKRWNLYRTIS
                     IGGLLLAATYLCLPFIGGFAAFTFIIVLVSFSEMLAMPFMNSYMNLKSSVASRGQYAA
                     LYSMAWSSAQTALPILATQTIANFGYNTLWILMACLLIIMTYIIRIVSQKMRTVAAN"
BASE COUNT          862 a          475 c          497 g          709 t
ORIGIN      
        1 cagatacact aaatctaact caaggcagat tacaacttaa tagtgttgcc ccaactgcaa
       61 tccgctcttt gagtttaatg agtaatacgg gttcaactac ggcattaact agaaatactg
      121 gtgtattgat agcagaatca aatccaccaa actatggcaa cttccgttgg aaaattggaa
      181 cacctgcggc ttatccaact acatttacat tcccgtttgc taacgctgca ggaacataca
      241 ttcctgttga ctataaagta aataatggcg atgctgatgt taccttaaca acttatacaa
      301 caatacctgc aaatacacca ataccaacag gcgtgtcaga tatttatatg aacacctggt
      361 ttgcaccgcc atttacagat aacagcccct atatggttga ccgtttctgg cagataaatg
      421 atgcaggtac agttagcagc cgcgacctta cattaaactg gacaccaaca gagaatgcat
      481 cagcaggctc gtctccttat cgtgcacagg tgtatagttc aagtactaat tggggttatc
      541 cttttatcat cggtcagaca aatccaacgg caacatcagt ttctattcct gcagctacct
      601 tcacaaacac atgggcagtt gtaagagaat tgcaaccgtt gcctgtagaa ttactttcgt
      661 ttgaagctaa accatataaa gatcaggaag tagtttgtaa ctggacaaca gctacagaat
      721 taaacaacga tttctttact gttgaacgca gcagcgatgg ccgtaacttc gaaaaagtgg
      781 gtacggtaaa aggtgccggt aattcaacgc aacaacacga ctataaattt attgatacta
      841 aaccttacag aggggtttct ttctacagac tgaaacagac agactttaac ggtaagtctg
      901 agtcgtttga gaaagtagct gtaaacttga gtttgcctgc aacaccaata agtgtttatc
      961 ctgttcctgc taacgaatat gtagtattca gtgttgacag aaatcagaat ctcgaaaatg
     1021 cagtattgta tgtttatgac tttacaggac gtgcattgat tcagaaaaaa gtagcagaac
     1081 ttaaaggtac aggaaccaat atctttatcc tacctgtaaa tactttaatc aatggtttgt
     1141 atcatttcga tttaaaagtt gagggagctt cattaggaaa cggtaaattt attgtagaga
     1201 aataattttt taaaagacaa agtgcccgta agtatgataa gtaaacaaac actgtcattc
     1261 ttacgggcac ttgccgttaa taacaaccgt gagtggtttg ctgcaaatca tcacaagtac
     1321 gagggtgcta aagaagattt taataattac ctcaaacaaa cgctaacgga attggcgaag
     1381 tacgatgttt tgctcaaaag ccttgaaccc aaagattgtg ttttcagaat ttatcgcgac
     1441 atacgttttt cgcccgataa aacaccttat aaaacaaatc tgggtgcagc tatcaatgcc
     1501 ggaggtaaaa gagtagaaac tgcaggagcc tatctgcaca ttcagcttgg caacaagtcg
     1561 ttttttgccg gtggacggtg gatgcctgat agggaaactt tacgaaaaat aaggcaggaa
     1621 attgactaca ataccggaga gtttaaagct attttgaatg ataaaaactt taaaaagtat
     1681 ttcggtacat tggaagatat agcgcttaaa aaagcaccta aagactatcc aaaagaccat
     1741 ccggaaatag cattgcttag atacacaagt tacattgcct ccatgccatt ggcagattca
     1801 ttaatcacgt ctgataaagg aataaaagaa attggtaaag cttataaagc attgatgcca
     1861 ttgctgcatt tcctgaatcg ggctttagat tagtttgcag caaccgttct cattttttga
     1921 gaaacaattc tgattatgta agtcatgatg atgagtaggc aggccatcaa tatccataac
     1981 gtattgtaac cgaagttggc tatggtttgt gtagcaagaa ttggcaaggc tgtttgtgcc
     2041 gaactccatg ccatagagta taatgctgca tattgtccgc gactggcaac agaacttttt
     2101 aaattcatat aagaattcat gaatggcatt gcaagcattt ctgaaaagga aaccaataca
     2161 ataataaaag tgaatgctgc aaaaccacca ataaaaggaa ggcaaaggta agtcgctgca
     2221 agcagaagtc cgccaattga tattgtacgg taaaggttcc atcgtttttc tatgcgataa
     2281 accaaaatca tttctacaac ggcaactatt aatccgttaa acatcatcag taaaccaatc
     2341 tgtctttcgc tcaggtgatg aatgtcttta taaaataaag gcatggttat aaaaaactga
     2401 aagaaacaaa gtgcatagat aaaagcagct acaataaaat aaagataaat tttgtctttg
     2461 taaggagaag caatttgctg tgtttggctt ttgccgagca catgatgcac ctttttatta
     2521 tgcagcatag atagtgtaaa tgt
//
LOCUS       DAOOOL010000253         2525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5101_length_2525_cov_72.266397, whole genome shotgun sequence.
ACCESSION   DAOOOL010000253 DAOOOL010000000
VERSION     DAOOOL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5101_length_2525_cov_72.266397"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>2525
                     /gene="carB"
                     /locus_tag="PKU82_12320"
     CDS             81..>2525
                     /gene="carB"
                     /locus_tag="PKU82_12320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOZ83693.1"
                     /translation="MPRDNSIKSVLIIGSGPIIIGQACEFDYSGSQASRSLREEGIEV
                     TLINSNPATIMTDQVTADHIYLLPLTVDSIEKILNERKIDAVLPTMGGQTALNLCKEA
                     DERGIWTKYGVRIIGVNVAAIETTENRESFRKLMIEIGVGVPPSNVANSFLEGKEIAQ
                     RIGFPLVIRPSYTLGGTGGSFVMKKEEFEDRLMRGLQASPIHEVLVEKAVLGWKEYEL
                     ELLRDANDNVIIICSIENLDPMGVHTGDSITIAPAMTLSDHTYQRMRDMAIKMMRAIG
                     KFAGGCNVQFAVSPDTEDIIAVEINPRVSRSSALASKATGYPIAKIAAKLAIGYHLDE
                     LKNQITGSTSAFFEPALDYVIVKIPRWNFDKFHGADRTLGLQMKSVGEVMGIGRSFNE
                     ALQKACQSLEINRHGLGADGTGWHKVDDIIYSLEHPSWDRIFHVKDALKLGLPIQTIH
                     NHTKIDKWFLTEIQKLVGMEQELRRHSLKNLSPDLMLELKQNGFSDYQIAHILGNVTA
                     EEVYETRKEMGVKRIYKMVDTCSAEFAAKTPYFYSTFERPAQDSNGNEINASESIVSN
                     KKKILVLGSGPNRIGQGIEFDYSCVHGVLASKESGYEAIMVNCNPETVSTDFDIADKL
                     YFEPVYQEHIFELIEYEKPDGVIVQLGGQTALKLSEYLNEKGIKIIGTSYEDMDLAED
                     RGRFSDLLHDLGIPYPPYGVAESAEEALVVANKVGYPVLVRPSYVLGGQGMSIVINDE
                     ELERAVIKLLKNLPGNRVLIDHFLDRAEECEADLICDGKDVQIIGIMEHIEPAGIHSG
                     DSYAVLPPFSLSDVVIS"
BASE COUNT          793 a          452 c          565 g          715 t
ORIGIN      
        1 ctccatacat ctgttgttca taagattgca tttcctgcat tgctgtttta gctaattttg
       61 cacagcaatt taagaaacag atgccaaggg ataattccat aaaatcagta ctcattatag
      121 gcagtgggcc tatcatcatt ggtcaggcct gcgagtttga ctacagcgga tcacaagctt
      181 cacgttcact gcgcgaagaa ggaattgaag tgacgcttat caactcaaac cctgccacaa
      241 tcatgaccga tcaggttacg gcagatcata tttatctgct gccgcttact gttgatagca
      301 tagaaaaaat attaaatgag agaaagattg atgccgtttt gccaactatg ggcggtcaga
      361 cagcattgaa tctttgcaaa gaagccgatg aaagaggcat ctggactaaa tatggtgttc
      421 gcattattgg tgtcaatgta gctgcaattg aaactacaga aaatcgtgaa tcgttccgta
      481 aactgatgat agaaattggt gttggtgtac caccatctaa tgttgccaac tcatttctgg
      541 aaggtaaaga aattgcacag cgtataggat ttccattagt aattcgtccc tcctatacct
      601 taggtggaac aggtggcagt tttgtaatga agaaagaaga gtttgaagac aggttgatgc
      661 gtggattgca ggcttcaccc attcacgaag tacttgtaga aaaagctgtc ctcggatgga
      721 aagaatatga attagaactg ctgcgtgatg caaacgataa cgtaattatc atctgctcta
      781 ttgaaaacct tgaccctatg ggtgtccaca caggtgacag cattaccatt gctccggcaa
      841 tgacattgag cgatcatacc tatcagcgaa tgcgcgatat ggcaattaaa atgatgcgtg
      901 ctatcgggaa atttgctggt ggatgtaacg tacagtttgc agtaagcccc gatacagaag
      961 atattattgc agtagaaata aatccacgtg tttcacgctc atcagcctta gcttcaaaag
     1021 caacaggcta tcccatagca aaaattgctg caaagcttgc catcggttat caccttgacg
     1081 aattaaagaa tcagattaca ggaagtactt ctgcattctt tgagcctgcc ttagactatg
     1141 ttattgtaaa aatacctcgt tggaatttcg ataagtttca tggtgccgac cgcacattgg
     1201 gtttacaaat gaaatctgtc ggtgaggtga tgggtattgg tcgcagcttt aacgaagcac
     1261 ttcagaaagc atgtcagagt ctggaaataa accgtcatgg tttaggtgca gacggaaccg
     1321 gatggcataa agttgatgac atcatttatt cacttgagca tccatcatgg gacagaatat
     1381 ttcatgttaa agatgcattg aaattaggtc ttcctattca gaccattcac aaccacacta
     1441 aaattgataa atggtttctg acagagattc agaaattagt aggcatggag caagaactga
     1501 gaagacatag tcttaagaat ctttctccgg acttaatgct tgaattaaag cagaacggat
     1561 tttcagatta tcagattgca catatattgg gaaatgtcac tgctgaagaa gtttatgaga
     1621 cccgaaaaga aatgggagta aaacgtattt acaaaatggt tgatacctgc tctgcagagt
     1681 ttgcagcaaa gacaccctat ttctacagca ctttcgaaag gccggcacaa gacagtaatg
     1741 gcaatgaaat taatgcaagt gaaagtattg tcagtaataa gaagaaaatt ttagtcttag
     1801 gcagtggccc taaccgtatt ggtcagggta ttgagttcga ctattcatgt gttcatggtg
     1861 tattagcatc aaaagaatcg ggctatgaag caataatggt taactgtaat cccgaaactg
     1921 taagtacaga ttttgacatt gcagacaaac tttattttga acccgtatat caggaacaca
     1981 tcttcgagct tattgaatat gaaaaaccag atggtgtaat tgttcaactt ggtggacaaa
     2041 cggctctgaa actatcggag tatctcaatg aaaaaggaat aaaaataatc ggcacttctt
     2101 atgaagacat ggatttggca gaagaccgtg gacgtttttc tgatctactt catgatttag
     2161 gaatacccta tcctccttat ggtgttgcag aaagtgccga agaagcattg gttgttgcca
     2221 ataaagttgg atatccggtt ttggttcgtc caagttatgt gttgggtgga caaggaatga
     2281 gcattgtaat caatgacgaa gaattagaac gtgcagtaat aaagctgctg aaaaatcttc
     2341 cgggtaacag agtgctcatt gaccattttc ttgatcgtgc agaagaatgt gaagcagatt
     2401 tgatttgtga tggcaaagat gttcagatta taggtatcat ggaacatata gaaccggcag
     2461 gcattcatag tggtgattct tatgcagtgt tacctccatt cagtttaagc gatgttgtca
     2521 tcagt
//
LOCUS       DAOOOL010000254         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5180_length_2500_cov_129.415951, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000254 DAOOOL010000000
VERSION     DAOOOL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_2500_cov_129.415951"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /gene="rsmG"
                     /locus_tag="PKU82_12325"
     CDS             complement(<1..375)
                     /gene="rsmG"
                     /locus_tag="PKU82_12325"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOZ83694.1"
                     /translation="MSSGVSLLQRYFPELPLEKQEKFVLFAELFKVWNEKINLVSRKD
                     IDQFYLHHLLHSLVIAKFIDFGKGTKVMDIGTGGGLPGLPLAIYFPETNFYLVDSIRK
                     KTEAVKEMANQLSLSNVTVFNKR"
     gene            complement(379..1032)
                     /locus_tag="PKU82_12330"
     CDS             complement(379..1032)
                     /locus_tag="PKU82_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ83695.1"
                     /translation="MEVKSPKTAKAAQDKTEVNPNLSDKAVVDYQLVREAVDHGNQKA
                     YAELMSRYKDSIYFMLLKMVNNRDDADDLTIEAFGKAFKNIHQYTPDYAFSTWLFKIA
                     TNNCIDYIRKKRKLMLSIDKGYENENGEDVALEIKSDTIGPEEKLMKKQKMETMREVV
                     DKLKPRYRTLIELRYFQELSYEEIATNMDLPLGTVKAQLFRAREFLYQIMKNSEHRP"
     gene            complement(1023..2024)
                     /locus_tag="PKU82_12335"
     CDS             complement(1023..2024)
                     /locus_tag="PKU82_12335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ83696.1"
                     /translation="MSEIEPPVSIIICARNELKNLKAYLQQVLEQDYPRFQVIVVNDC
                     SWDESEKYLEELSATYTNLKVVSIKEQERYQHGKKFALTIGIKSALYDHLLLIDADCY
                     PASNQWIRLMMQSYNAKTRIVIGYGAYIKTSSLLNRWVRFDTAFNAMQYLSFALAGNP
                     YMGVGRNLSYQRSLFFENKGFARHQHIMSGDDDLFINDTATTENLSVQINPDSFTYSQ
                     PKTDFIDWFRQKKRHISASRLYKFKHKMLLGIYYFSHISFLVLFIALLIIQFKIEYVV
                     TAFSLRLVFQMIIFGSCLKKLKEGDLIPLIPIFDIWIAVVYPLIAISNRLIKNKTWK"
     gene            2283..>2500
                     /locus_tag="PKU82_12340"
     CDS             2283..>2500
                     /locus_tag="PKU82_12340"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOZ83697.1"
                     /translation="MHFSIEKYDKESKARSGRIETAHGIIETPIFMPVGTAGTVKSIN
                     QRDLKQDVKAQIILGNTYHLNLRPGIEV"
BASE COUNT          818 a          444 c          413 g          825 t
ORIGIN      
        1 tcgtttgtta aatacagtta cattagataa tgaaagttga ttggccattt ctttaacggc
       61 ttcagtcttt ttacgaatag aatcaaccag ataaaagtta gtttcaggaa aataaatagc
      121 taatggtaaa cccggtaatc cgcctccggt gccaatgtcc atgactttag ttcctttacc
      181 aaaatcaata aactttgcta tgacaagaga atgtaataag tggtgtaaat aaaactgatc
      241 tatatctttt ctggaaacaa ggtttatctt ctcattccat actttaaata actcagcaaa
      301 taagacaaat ttttcctgct tttctaaagg taattccgga aagtagcgtt gcaataatga
      361 aactccgctt gacatctttt atggacgatg ttcagagttt ttcattatct gatacagaaa
      421 ttcacgcgcc ctgaaaagtt gggctttaac agttccaaga ggtaaatcca tgttcgttgc
      481 aatttcttca taactcaact cctgaaaata acggagttca ataagtgtcc ggtatcttgg
      541 ttttaactta tcaacaacct cacgcatggt ttccattttt tgctttttca tcagcttttc
      601 ttcaggacct atggtgtcag attttatttc caaagcaaca tcttcgccat tttcgttttc
      661 atatccttta tcaatactca acattaactt tcgctttttg cggatataat caatgcaatt
      721 gttggttgct attttaaata gccaggtgct gaaagcatag tcaggtgtat attgatgaat
      781 attcttaaat gctttgccaa aagcttcaat tgtcaaatca tcagcatcgt cacgattatt
      841 gaccatttta agtagcataa aatagataga atccttgtag cgcgacatta gttcagcata
      901 agctttttga ttgccatgat ctactgcttc acgaaccagc tgataatcta caactgcttt
      961 atcacttaag ttaggattaa cttctgtttt atcttgtgct gcctttgcag ttttgggact
     1021 ttttacttcc atgttttatt ctttattaaa cgattggaga tggcaattaa aggataaaca
     1081 actgctatcc atatatcgaa tatcggtatc aacggaatca gatcgccttc ctttaatttt
     1141 tttaagcatg agccaaaaat aatcatttga aaaaccaaac gtaaactaaa ggcagtaact
     1201 acatattcaa ttttaaactg aattattaac aatgctataa ataaaacaag gaatgaaata
     1261 tgtgaaaaat agtaaatgcc taaaagcatt ttatgtttaa acttatataa gcgagaagca
     1321 cttatatgcc ttttcttctg acggaaccag tcaataaagt cggtttttgg ctgtgaataa
     1381 gtaaaagaat cggggtttat ctgaacagaa agattctctg tagtggctgt atcatttata
     1441 aaaagatcat catcgccaga cataatatgt tgatgtcggg caaagccttt attctcgaaa
     1501 aacagtgaac gctgatatga taaatttctt ccaacaccca tgtaagggtt tcctgcaagt
     1561 gcaaatgaaa gatattgcat tgcattaaat gcagtgtcaa atctaaccca acggtttaat
     1621 agtgaagaag ttttaatata ggcaccataa ccaattacta ttcttgtttt tgcattataa
     1681 ctttgcatca tcagtcgtat ccactgattg cttgccggat aacaatctgc atcaatcaac
     1741 aacagatggt catataaggc agacttaatt ccaattgtca aggcaaactt ttttccatgc
     1801 tgataacgtt cctgttcttt gattgaaact actttaagat ttgtataggt tgcagatagt
     1861 tcttcaagat acttttctga ttcatcccac gaacaatcat tcacaactat aacctgaaat
     1921 cgaggatagt cttgctccaa tacttgttga agatatgcct ttaggttttt cagttcattt
     1981 ctggcacaaa ttattattga aacaggcggc tcaatttctg acactggggc gtttactgtt
     2041 ttatgcagag acaaacgcac aaaaacaaat aaataataga ttagttgtat ggtaataaat
     2101 actacaagca aataaaaaag taccaatgaa atattttcaa tgcttataaa atctggtata
     2161 ggtatgggta tcaaaataac tttggtgttg gtaagcgcgc aaatgtatga agagtcattg
     2221 cagaaggctt ttctatcttt gtaaaaatat taacaacact ttttgcaaaa attactgaat
     2281 ctatgcattt ttctattgaa aagtatgata aagaaagtaa agcccgctcg ggcagaatag
     2341 aaaccgctca tggcattatt gaaactccaa tttttatgcc tgttggcaca gcaggaacag
     2401 tcaaaagcat taatcagcgc gacttaaaac aggatgttaa agcacagatt attcttggca
     2461 acacctatca tctgaattta cgtcccggca ttgaagtttt
//
LOCUS       DAOOOL010000255         2499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5183_length_2499_cov_66.441080, whole genome shotgun sequence.
ACCESSION   DAOOOL010000255 DAOOOL010000000
VERSION     DAOOOL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5183_length_2499_cov_66.441080"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..750
                     /locus_tag="PKU82_12345"
     CDS             103..750
                     /locus_tag="PKU82_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOZ83698.1"
                     /translation="MELYKELSETQIAERILNGEKSLYEIIVRRFNPTLYKIGRSYNY
                     NHEDTEDLMQDTFVDAYRNLSQFEGRSNFKTWIIRIMLNNCFRKKEKSGFKNEIKQDV
                     HENSQPMFTGTNNDTQKLIQNRELGDIIEEALSKIPDEYRMVFSLREINGMNVSETAG
                     LLNISEANVKTRLNRSKAMLRAEIEKSYAAEELFEFNAVYCNAMTDRVMKIIDKL"
     gene            1107..>2499
                     /locus_tag="PKU82_12350"
     CDS             1107..>2499
                     /locus_tag="PKU82_12350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOZ83699.1"
                     /translation="MNFTKALFAAIISIALLSSCKTTQKTPVETHDLPAIEVKPPYED
                     YRASNTILNDLQHTKLKVRFDWNKKYLYGIAEVTAKPYFTSVNTLILNARGMDFNEVS
                     LITDTGRKKLTYSYQNDSLTINLDRFYNRTETYTVYIDYISKPDELKNIGGSVAINSD
                     KGLYFVNADGKNPNKPRQVWTQGETQSNSVWFPTIDSPNQNMTQEISITVDSSFVTLS
                     NGLMTSSVKNADGTRTDTWKQSLPAAPYLTMMAVSNFKVVKDRWRNIEVNYYVDPEYE
                     MYAKDIFGNVPEILDFYSRKLGVDYPWEKSSHVVVHDYVSGAMENTSAILYGEFLQRT
                     KRELLDGTSESTIAHEIFHHWFGDYVTCESWSNIPLNESFATYGEYLWDEYKYGREEA
                     DKGGMDDLNQYLAEAKYKQVDLIRFHYNAREDMFDRHSYSKGGRVLHMLRKYVGDDDK
                     SAIEMIAAKRAFVK"
BASE COUNT          871 a          482 c          457 g          689 t
ORIGIN      
        1 tccttatggg tttcaactat gtagatgttg ccgttcgtga tttggtaatg gcaatatctg
       61 ccttttcaat ggcacaacta gctaaatttg taaaataata atatggaact ttataaagaa
      121 ttatccgaaa cacaaattgc ggaacgtata ttaaacggag aaaaatcgct ttacgaaatt
      181 atagtaaggc gatttaatcc tactctttat aaaattggtc gttcttataa ttacaatcac
      241 gaagacacag aagatcttat gcaggatact tttgtagatg catatagaaa tctttcacaa
      301 tttgaagggc gatcgaattt taaaacctgg attatccgaa taatgttgaa taactgtttc
      361 cgtaaaaaag aaaaatcagg ttttaaaaat gaaatcaaac aagatgtaca cgaaaattca
      421 caaccaatgt ttacaggaac aaataacgat actcaaaaat taatacaaaa tcgtgagttg
      481 ggagatatta tagaagaagc actaagcaaa attcctgatg aatacagaat ggttttctcc
      541 cttcgcgaaa tcaatggcat gaacgtatca gaaactgccg gactcttaaa catcagcgag
      601 gccaatgtga aaacacgact caaccgttct aaagctatgc ttcgtgcaga aatagaaaaa
      661 tcgtatgctg cagaagagtt gtttgagttt aatgctgttt actgtaatgc tatgacagac
      721 cgtgtcatga aaattattga taaactttag gttttaaaaa catcattaca tgaaaacaaa
      781 attattctca ccaagacaca aagaaataaa acacgcagag gccgctaaga aatatatctc
      841 ctttgcgtga aaagaaaacg agacttacta caaatacccc tttgcgagct ttgcgtgaaa
      901 aaaaaggtct ctcgccaaga cgcaaagaaa taaacacgca gaggccgcta agtgaatttc
      961 atccttgcgc tctttgcgtg aaatcaaaaa agtctctcgt caagatgtga agcaccccct
     1021 ttgtatcact ttttgctcct taacttgatt ttaaccaatt tagcagcata attttcatta
     1081 tttcacgcct tcaaattttc ctgaacatga attttacaaa agctctcttt gctgcaatca
     1141 tctctattgc acttttatca tcatgtaaaa caactcaaaa aactcctgtt gaaacacatg
     1201 accttcctgc catagaagta aaacctccct acgaagacta tcgcgccagc aacaccatct
     1261 taaacgactt gcaacatacg aaactaaaag tacgtttcga ctggaataaa aagtacctgt
     1321 acggcattgc cgaagtaacc gctaaaccct attttaccag tgtaaacacg ctgatactaa
     1381 atgcacgtgg catggacttc aacgaagtca gcctgattac cgacaccgga agaaaaaagc
     1441 taacctactc ctaccaaaac gattcattaa ccatcaacct cgaccgcttt tacaacagaa
     1501 ctgaaaccta cacagtttat attgactata tatcaaagcc tgatgaacta aaaaacattg
     1561 gtggcagtgt agcaattaat agtgataaag gtctttactt tgtaaatgcc gatggaaaaa
     1621 atcccaataa accacgacag gtttggacac aaggcgaaac acaaagcaac tccgtttggt
     1681 tccctactat tgattctcca aatcaaaaca tgactcagga aatcagcata actgttgaca
     1741 gctcatttgt aacacttagc aacggcttga tgacttcttc agttaaaaat gctgacggta
     1801 cacgtacaga tacatggaaa cagtcattgc ctgctgcacc ctatctgacg atgatggctg
     1861 ttagcaattt taaagtggtt aaagaccgtt ggcgcaacat tgaagtcaac tactatgttg
     1921 acccggaata tgaaatgtat gcaaaagata tttttggaaa cgtgcccgaa atactcgatt
     1981 tctactctcg taaattaggc gttgactatc catgggaaaa atcatcacat gttgttgttc
     2041 atgattatgt gagtggtgcc atggaaaata cttctgcaat tctttatggt gagtttttac
     2101 agagaacaaa acgcgaactg cttgatggca caagtgaaag tacgattgca catgaaattt
     2161 ttcatcattg gtttggcgat tatgtaacgt gtgagtcgtg gagcaacata ccattaaacg
     2221 aatcgtttgc aacttatggc gaatacctgt gggatgaata caaatacggt cgtgaagaag
     2281 ctgacaaagg cggcatggat gatttgaatc aatatcttgc tgaagccaaa tacaaacagg
     2341 ttgatcttat tcgctttcat tacaatgctc gtgaagatat gttcgacaga cacagctatt
     2401 caaaaggggg tcgggtgtta cacatgcttc gtaaatatgt tggcgatgat gataaaagtg
     2461 caatagagat gattgcagca aagagagctt ttgtaaaat
//
LOCUS       DAOOOL010000256         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5207_length_2493_cov_54.693191, whole genome shotgun sequence.
ACCESSION   DAOOOL010000256 DAOOOL010000000
VERSION     DAOOOL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5207_length_2493_cov_54.693191"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKU82_12355"
     CDS             complement(<1..782)
                     /locus_tag="PKU82_12355"
                     /inference="COORDINATES: protein motif:HMM:NF039394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HOZ83700.1"
                     /translation="MRLVVLLLFVFTQSVVAQPLIDIAALNYSYHRPFKFTNNESAIL
                     QYTNAFLTLPIKSGNNIFIINPSFDRFDFRFHQQSKLYMSGQLALTWQHQWKDKRFKT
                     AFVFIPKSISESKYWLQEGSYQLGGAILQSYQRSEQLSYKLGLYYNSEFFGPYFLPLL
                     GIDYRITPRWNLWGILPSSLNAEYKASINKLHFLISLKTMTLSYRKDDSYYLRTNDNL
                     LRIASDFYFAKNNVLFLETGHSVFRKFVLGTKNRDIDIQSTM"
     gene            881..1504
                     /locus_tag="PKU82_12360"
     CDS             881..1504
                     /locus_tag="PKU82_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOZ83701.1"
                     /translation="MFKIINKILIVLLLPMATNAQSFKASIIGGINASQVSGDELGGF
                     NKAGVMFGGSAILPVTPKSDVAMELLFIQKGSKTPTSKDNSNTVYYKMSLNYLEVQIN
                     YTYHASKKIGLHVGPTFGVLVGEKEEDIAGELTERPEFQKTEIGIAGGLSFHFSEKVG
                     LYMRLSNSLLPIRKMGADTKYFKSGQYNSGLAFFLTYTFSSKDKQKQ"
     gene            1504..2064
                     /locus_tag="PKU82_12365"
     CDS             1504..2064
                     /locus_tag="PKU82_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOZ83702.1"
                     /translation="MKNKIVVAVTGASGSIYASMLLSKLAQLKEQFSDVAIVFSKNAT
                     DVWQHELGNDSYKSLSFKRYDTMDFNAPFASGSAQYNTMIIIPCSMGTMGRIAAGISN
                     DLITRAADVILKERRKLILVTRDMPLNLIHINNMKTITEAGGIICPACPSYYSKPQTI
                     NDVAATVVDRALDLAGFSLNSFRWNE"
     gene            2084..>2493
                     /locus_tag="PKU82_12370"
     CDS             2084..>2493
                     /locus_tag="PKU82_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83703.1"
                     /translation="MISNLVFIRLQQLKRELTKTGWLYSLILLVITGLLGFLLYHVVQ
                     NSTHSFYLAALMIVSVYTIHSTRNDLQFIRQQLRNPFFILTTEYILLALPVIILLFLK
                     SYFIAGCTIIAGCILIALIVPKNSTHLLFPKLAR"
BASE COUNT          816 a          413 c          477 g          787 t
ORIGIN      
        1 ctcatagtgc tttgaatatc tatatcgcga ttcttagtgc ctaaaacaaa ttttctgaat
       61 acagagtgac ctgtttctaa aaacaataca ttattcttcg caaaataaaa atcggaagca
      121 atgcgtagca gattatcatt tgttcttaaa tagtaactgt cgtccttgcg gtatgaaagt
      181 gtcattgttt ttagcgaaat caaaaaatgt agtttgttga tgctggcctt atattctgca
      241 ttgagcgaag acggaagaat accccataaa ttccatcgtg gtgttatgcg ataatcaata
      301 ccgagcaatg gcaaaaagta aggaccgaaa aattcgctgt tgtaatataa tccgagtttg
      361 taacttagtt gctctgagcg ctgatagctt tgtaaaattg ctcccccaag ctgatagctg
      421 ccctcttgca gccaatattt actttcggaa atagatttcg gaataaacac aaaggcagtt
      481 ttaaaacgtt tgtctttcca ctgatgttgc catgttagtg caagctgacc actcatataa
      541 agttttgatt gctgatggaa cctgaaatcg aacctatcaa aagaggggtt gatgataaaa
      601 atattgttgc cgcttttaat aggtagtgtc agaaaagcat tggtgtactg aagtattgca
      661 gattcgttgt tggtgaattt aaatgggcga tggtagctat agtttaatgc tgcaatgtca
      721 attaatggct gcgccactac cgactgagta aaaacaaaaa gcaatagaac tacaaggcgc
      781 atatttactg tttatttaat gcaagattaa cacttgaact gtaaataacc aacctttaca
      841 acctgttaat ttttactttt gttgtacata acatcgcact atgtttaaaa ttattaataa
      901 aattctgatt gtgcttttat tgccaatggc tacaaatgcg caatcattta aagcttccat
      961 cattggtggc atcaatgcat cacaagtttc cggtgatgag ttgggaggtt ttaataaagc
     1021 gggagtaatg tttggaggta gtgccatatt acctgtcaca ccaaagtcag atgttgcaat
     1081 ggaattgttg tttattcaga aaggaagtaa aacgccaaca tcaaaagaca atagcaatac
     1141 cgtctattat aagatgagtt taaattatct ggaagtgcaa attaattata cctatcatgc
     1201 atctaaaaaa ataggcctgc acgttggacc tacgtttggt gtattggttg gtgagaaaga
     1261 agaagatata gcaggtgagt tgacagaaag accggaattc caaaagacag aaataggtat
     1321 tgcaggcggg ctatcatttc atttcagcga aaaagtgggt ttgtatatgc gcttatcaaa
     1381 ttcattgttg cctattcgta aaatgggtgc cgacacaaaa tattttaaga gcggacaata
     1441 taatagtgga ttggcatttt ttcttactta cacttttagc tcaaaagaca aacaaaagca
     1501 ataatgaaaa acaagattgt agttgccgtt accggtgcca gtggaagtat ttatgcatca
     1561 atgttgctta gtaaattggc acagctaaaa gaacaatttt ctgatgttgc aatagtgttt
     1621 tcaaaaaatg caacagatgt gtggcagcat gaattaggta atgattccta taaatcactt
     1681 tcctttaaga gatatgatac aatggatttt aatgcacctt ttgcatcggg atcggcacaa
     1741 tacaacacta tgattattat tccttgcagc atgggaacaa tgggacgcat tgctgcagga
     1801 atttcaaacg acctgattac acgtgctgcc gatgtgatac taaaagagcg aagaaaactt
     1861 atcttagtta caagagatat gccactgaat ctgattcata tcaacaacat gaaaactatt
     1921 actgaagccg gagggataat ttgccctgca tgcccatcat attacagcaa gccacaaacc
     1981 atcaatgatg tggcagcaac tgtagttgac cgtgcattag accttgccgg ttttagtttg
     2041 aacagcttcc gttggaatga ataaacagtc ttcggaattt caaatgattt caaatcttgt
     2101 ttttatcaga ctgcagcaac ttaaacgcga gttgacaaaa acagggtggt tatatagtct
     2161 tatactatta gtcattacag gacttttagg atttttactt tatcatgtag tacaaaattc
     2221 tactcatagt ttttatttag cagcattgat gattgttagt gtctatacta ttcatagtac
     2281 aagaaatgat ttgcagttta tcagacaaca acttagaaat ccatttttta ttctgacaac
     2341 tgaatatatt ttgttagcac ttcctgttat cattctgtta tttttaaaaa gttattttat
     2401 tgcaggatgc acaataattg caggatgcat attgatagct ttaatagtac caaaaaattc
     2461 aacacattta ctttttccaa aacttgcaag gtg
//
LOCUS       DAOOOL010000257         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5220_length_2489_cov_71.495481, whole genome shotgun sequence.
ACCESSION   DAOOOL010000257 DAOOOL010000000
VERSION     DAOOOL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5220_length_2489_cov_71.495481"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PKU82_12375"
     CDS             complement(<1..1312)
                     /locus_tag="PKU82_12375"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOZ83704.1"
                     /translation="MKKTITLFICLFSLSLATWAQDKTVTGKVTSSQDKLGIPGVSIL
                     EPGTTNGTVSDIDGKYSIKVKSTTTQLRFSGTGLTTRTVDIPASNSLDLTMQPDVQKI
                     GEVVVTALGIKREEKSLGYSTQKVSGDDLAAAKEANVINSLQGRVAGVSITGSSNMGG
                     SSRILLRGMRSINFENQPLFVVDGIPMNNENVATVDQQRGALGYDYGNAIQDINPDDI
                     ESINVLKGPSATALYGSRGSNGVIIITTKKGVAREKSEKYSPIGVTLSTGWSMKKIYN
                     LPKYQNRYGGGASSDFIESDIHPGELRSEFEYDGSWGPELLGQEVYQWDSYVPSMANY
                     NKKTPWVAHPNNVKDFFETGIVKNNSVSLDGGNEKSLFRLGYTNYDEKGVIPNESLNR
                     NIISFNGSNKFNDRLSATLNMNYVVARGKGRAATGYNSIASNFNQ"
     gene            complement(1568..>2489)
                     /locus_tag="PKU82_12380"
     CDS             complement(1568..>2489)
                     /locus_tag="PKU82_12380"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOZ83705.1"
                     /translation="LYIILVFFTPENGWLSLFLASLLGLVMASIGFNVMHDGAHGSYS
                     SHKWVNESMAHSLNLLGGVSFIWKQKHNINHHSYTNVEGMDDDIDIKPFIRVHEGQKR
                     YWFHRYQHIYGLLLYGITYFFWVFYNDFNKYFSGKIAEHTHMQKMSIWDHVIFWATKI
                     FYVLVFIVFPIYNVGFIDTLLGYGVMVAVTGITIAVVFQLAHVVEDMPFVSPEGDVMK
                     IESEWAVHQLQTTTNFATKNKFISWLLGGLNFQVEHHLFPRISHIHYPSISKIVRETC
                     LEYNVQYKEFRTMRGALASHILHLREMGRK"
BASE COUNT          783 a          501 c          423 g          782 t
ORIGIN      
        1 actgattgaa gttagaggca atactgttat atcctgttgc agcacgacct ttacctcttg
       61 caacaacata gttcatattt aatgtagcag aaagcctatc attaaattta ttggagccat
      121 taaagctgat gatatttctg tttaggctct cgtttggaat aacacctttt tcatcatagt
      181 ttgtataacc taatctgaaa agtgattttt cgtttccgcc atctaaagaa acactgttgt
      241 ttttaacgat acctgtttca aagaaatctt tcacgttgtt tggatgagct acccatggtg
      301 ttttcttatt gtagttggcc attgaaggaa cgtagctgtc ccactgataa acttcctgtc
      361 ctagtaattc aggaccccag cttccatcat attcaaactc tgagcgtaac tcgcccgggt
      421 gaatatcact ctcaataaaa tctgaagatg cgcctccacc ataacggttc tgatatttag
      481 gaagattata aattttcttc atgctccatc ctgtacttag tgttacaccg attggagaat
      541 acttttctga tttttcgcgg gcaacacctt ttttagttgt aatgatgata acaccgtttg
      601 aacctcttga accataaagt gctgttgcag atggcccttt aagtacgttt atagattcaa
      661 tgtcatcagg gtttatatcc tgaatggcat taccataatc ataacctaat gctccacgtt
      721 gctgatcaac tgtggccacg ttttcgttat tcattggaat accatcaacc acaaataaag
      781 gttggttttc aaagttgata gaacgcatac cgcgcaataa tattcttgaa gaaccaccca
      841 tattacttga tcctgtaatg ctaacaccgg caacacgacc ttgcaaagag ttgataacgt
      901 ttgcttcttt tgcagctgcc aaatcatcgc cacttacttt ttgtgtactg tagcctaatg
      961 acttctcttc acgtttaata cctaaggctg ttactactac ttcaccaatt ttttgaacat
     1021 caggctgcat agtaagatct aatgaatttg atgccgggat atcaactgtg cgcgttgtta
     1081 aaccggttcc ggaaaaccga agttgcgttg tagttgattt aactttaatg gaatattttc
     1141 catcaatatc cgaaacggta ccattggtgg ttccgggctc gaggatgctg acaccgggaa
     1201 tacccagttt gtcctgcgat gaagtaacct taccggttac tgtcttgtcc tgtgcccagg
     1261 ttgccaatga caacgagaac aggcaaataa acaatgtgat tgttttcttc atactgcttt
     1321 taatttgagt ttaacttatt taatttattt gttgtagaat gaaactggcg gtttaatcta
     1381 ctgtctgtat caatgaaaaa cagagccttt ttatattgtg aataaccttg aatgaaagat
     1441 aatttcatgt atgtggtttc agaaaaactt cacacaaagt taataataat ttgaattaac
     1501 caaattttta cgaaaaattt acaacgtata agtaataact gagctaaaca ggcaaaaaca
     1561 acgatgctta tttcctgccc atttcgcgca gatgaagaat atgcgatgcc aatgcaccac
     1621 gcatggtacg gaattctttg tattgtacat tatattccaa acatgtttcg cgcacaattt
     1681 tactgattga tggatagtgt atgtggctta tgcgtgggaa caaatgatgt tcaacctgaa
     1741 aattcaaacc acctaacaac caactgataa atttattttt tgttgcaaag ttggttgttg
     1801 tctgtaactg atgcacagcc cactccgact caattttcat tacatcacct tcaggactaa
     1861 cgaatggcat atcttcaaca acatgtgcca actgaaaaac aacggcaata gtaattcctg
     1921 taactgctac catcacgcca taacctaaaa gcgtatcaat aaaacctaca ttatatattg
     1981 ggaaaacaat aaacactaaa acataaaata ttttagtcgc ccagaaaata acatgatccc
     2041 aaatagacat cttctgcatg tgggtatgtt cagctatttt accggaaaaa tatttattaa
     2101 aatcattata aaaaacccag aagaaataag ttattccata caaaagcaaa ccataaatat
     2161 gctgatagcg atgaaaccaa tatctcttct gaccttcatg cacgcgtata aaaggtttga
     2221 tgtctatatc atcatccata ccttcaacgt tggtatagga atgatggttg atgttatgtt
     2281 tttgtttcca gataaatgaa acgccaccca gcaaattaag cgaatgtgcc attgattcat
     2341 ttacccattt atggctggaa taactgccat gtgcaccatc gtgcattaca ttaaatccaa
     2401 tagatgccat taccaaacct aaaagcgaag ctaaaaacaa ggacagccat ccgttttcag
     2461 gggtaaaaaa taccagtata atataaagc
//
LOCUS       DAOOOL010000258         2465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5290_length_2465_cov_54.640664, whole genome shotgun sequence.
ACCESSION   DAOOOL010000258 DAOOOL010000000
VERSION     DAOOOL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5290_length_2465_cov_54.640664"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKU82_12385"
     CDS             <1..1088
                     /locus_tag="PKU82_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOZ83706.1"
                     /translation="IMPVDGVPKHLVKTITRAKFEQLVDDLIKRTIEPCRKALENAGL
                     KASDIDEVILVGGSTRIPGVQSAVEKFFGKSPSKGVNPDEVVAIGAAIQGAVLTGEVK
                     DVLLLDVTPLSLGIETFGGVFTKLIEANTTIPTKKSEVFSTASDNQPSVEIHVLQGER
                     SMAKDNRTIGRFHLDGIPPAPRGVPQIEVIFDIDANGILHVTAKDKGTGKEQKIRIEA
                     SSGLSDDEIKRMKTEAEANAEADKKAKEEADKVNMADTMIFQTEKQLTEYGDKLSQGN
                     KENIQRALSKLKDAHKEKNLADIDTALNELNTAWQGASQEMYNATNNTANTTAGGADA
                     NANAEAQNNTAGEVTDVDFEEVKDDKK"
     gene            1261..1797
                     /locus_tag="PKU82_12390"
     CDS             1261..1797
                     /locus_tag="PKU82_12390"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HOZ83707.1"
                     /translation="MIIPLSLIAQEQNQQYILDILPSENGYVKMVSVSAVENTAKNEL
                     YKRAYRWVVDNIRSAKDAIDYADTTTAQLVVKTNFDIDATTAMIKTTHTISCSIYIDI
                     KDEKYRCVIKDFNIKERRPDIKRGYLPTIKDYQLETWLELKPNVNKKYAQEILNKLLI
                     MQNSFANAMKKPIVSDGF"
     gene            complement(2096..2359)
                     /locus_tag="PKU82_12395"
     CDS             complement(2096..2359)
                     /locus_tag="PKU82_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOZ83708.1"
                     /translation="MYFVYVLYSESGSRHYIGQTNDISTRLHRHNSGYEKSTSPYVPW
                     KLLCKIEKQSRSEAMILERKLKNLNTEDLRKFIQKYGIKPTGG"
BASE COUNT          849 a          456 c          495 g          665 t
ORIGIN      
        1 acattatgcc tgttgatggt gttccaaaac acttggtaaa aaccattaca cgtgcaaaat
       61 ttgagcagtt ggttgacgat ttaatcaagc gtacgattga accatgtaga aaagcattag
      121 aaaatgcagg attaaaagct tctgacattg acgaagtgat tttagttggt ggatcaacac
      181 gtattccggg agttcaatcg gcagtagaga aattcttcgg taagtcgcct tcaaaaggcg
      241 ttaaccccga tgaggtagtt gccattggag cagccataca aggagctgtt ttaaccggtg
      301 aagtgaaaga tgtgttgtta cttgacgtaa ctccattgtc actcggtatt gaaacatttg
      361 gcggtgtgtt taccaaactt attgaagcta acacaaccat accgactaag aaaagtgaag
      421 tgttcagcac tgcaagcgac aatcagccat cggtagaaat tcacgttctg cagggcgagc
      481 gcagcatggc aaaagacaat cgtaccatcg gacgttttca cttagacggt attccacctg
      541 caccacgcgg tgttccacag attgaagtaa tattcgatat tgatgcaaac ggaattctgc
      601 atgtaacagc aaaagacaaa ggtacaggca aagaacaaaa aatacgtatt gaagcaagca
      661 gcggattgag tgatgatgaa atcaaacgta tgaaaacaga agccgaagcc aatgcagaag
      721 ctgataaaaa agcaaaagaa gaagctgata aagttaacat ggccgacacc atgatattcc
      781 agacagaaaa acaactgact gaatatggcg ataaactttc gcagggcaat aaagaaaata
      841 tacagcgtgc actgagcaaa ctcaaagacg cacataagga gaaaaacctt gctgatattg
      901 acactgcact gaatgagttg aacacagcat ggcaaggtgc atcgcaagaa atgtataatg
      961 ctactaacaa cacagccaac acaacagcag gtggtgcaga tgctaatgct aatgcagaag
     1021 cacaaaacaa cactgcaggc gaagtaactg atgttgactt tgaagaagta aaagacgata
     1081 agaaataaaa gtagatttgg tttttagtta gaccggctca gacaactgag ccggtttttt
     1141 gttttagggt atataatgag ctaaaaatta tcttccttgt aactttggtt taatgccagt
     1201 tccttttaca tttgtgagat atcgtatttg aaatttatta aatcattaat tattgcattt
     1261 ttgattatac ctttaagttt gattgctcag gaacaaaatc aacagtatat tttagatatt
     1321 cttccgtccg aaaacggtta tgttaaaatg gtttctgtat cagcagtaga aaatacagct
     1381 aaaaacgaat tatacaaaag agcctacagg tgggtagttg acaatattcg gtcagctaaa
     1441 gatgctattg actatgccga taccacaacg gctcaacttg tggttaaaac caactttgac
     1501 atagatgcaa caaccgcaat gattaaaaca acacatacaa tttcatgctc aatatatatt
     1561 gacataaaag atgaaaagta tagatgtgtg attaaggatt ttaatattaa ggaacgaaga
     1621 cctgatataa aaagaggtta tcttcctaca atcaaagatt atcagcttga aacatggctt
     1681 gaattaaaac ctaatgtaaa taaaaaatac gcacaagaaa tactcaataa gttattgata
     1741 atgcaaaaca gttttgctaa tgccatgaaa aagccaattg tttctgatgg attttaaaca
     1801 acttgtaaaa ttaagataac ttttaccaaa ggataatctt gatatctcaa gatatgaatt
     1861 cccttaaact aaaaaagcct cctatcggaa gcctcttttg cggagagaga gggatcgaat
     1921 agcgaggctt gctcgctatt gagagtgaca acggaacctc acaagggcga ggcttgctcg
     1981 ctattgagag tgacaacgga acctcacaag ggcgaggctt gctcgccatt gagagtgaca
     2041 aagaaacctc acaatagcgg ggcttgctcg ctattgagag tgacattgtc acctctcaac
     2101 cgcccgttgg ttttattcca tacttctgta taaactttcg taagtcttcg gtattcaaat
     2161 ttttcaactt ccgttccaaa atcattgctt cagacctact ctgtttttct attttacaaa
     2221 gtaacttcca tggtacatat ggcgatgtag atttttcata gccactgtta tgtctatgta
     2281 atcgtgtgct aatatcattt gtctgaccta tataatggcg actaccgctc tcagaatata
     2341 aaacgtaaac aaaatacatt ttctcattta ctccttaaac taaaaaagcc tcctatcgga
     2401 agcctctttt gcggagagag agggattcga acccccgttg gttttaccca aggcggtttt
     2461 caaga
//
LOCUS       DAOOOL010000259         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5381_length_2436_cov_24.151617, whole genome shotgun sequence.
ACCESSION   DAOOOL010000259 DAOOOL010000000
VERSION     DAOOOL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_2436_cov_24.151617"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKU82_12400"
     CDS             complement(<1..350)
                     /locus_tag="PKU82_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83709.1"
                     /translation="MATRKETGEVEIRLSTEQAQKRIRELETEARKLEKAIDAAATGE
                     PGKRDALYEMLEKNQAEVQKLRESMKTDMRIIINGEVAGQNIKNLENALKQLQREFRA
                     VGGDDAALKQKAAQ"
     gene            complement(360..803)
                     /locus_tag="PKU82_12405"
     CDS             complement(360..803)
                     /locus_tag="PKU82_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83710.1"
                     /translation="MGAEPITELQLVTQWLQYVVENEQELLEKYTRSERRRLYNSFVK
                     SIDAHGDKVSRIHLRFYKYLRYLDMGVGRGVASGSRFVNKNFYKKRNRLGQLHKYKRK
                     KIPVYNKPITAETKRLLELLADNFNIKAITTLENMMQKNEITINL"
     gene            complement(816..>2436)
                     /locus_tag="PKU82_12410"
     CDS             complement(816..>2436)
                     /locus_tag="PKU82_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83711.1"
                     /translation="VYLHHIIKSGFAFDGYSVDDTVGFMADATKKNLLVYTNQSIDEK
                     GSLTGFGMLAGTIPTWPLTTHTAAMGEHTLIFNNDSTGGCYDSLGAYNPTTGHYTIQA
                     TGQHLIKVNLNIRGFNMSSPGVYPTYKLKLYEDGVVIHTFLSNQLSQAGSLSVWNASW
                     WFTANAADVGKDLWLTFEIVMPGGTNPSGYFRLDYSYFGVNDLPNTTGFNVFKRYWNM
                     RDQVPDITFGQLLNAIKETYNLTYKIDRVNKIVRIHYFDELLQQPQRDFSNQATSNHE
                     IKFDNSIVIKSFNYTFGGNDSSSADSWKEYDINKFWGEADNVDVLINNDATLLGYFAY
                     VKCLHQIWKCNDLGSSVYKWQYYCEGYKDFVVNEKGAVEIRPQLSPLFTLRRVYYDSG
                     TPHYQILPRIKQLGSSKEFGLGDNPYDLHLVHYLGIVSGFCDLGPIPTASSVCITTYG
                     MDLQHNLVWNKSNLLYYWQNLVYILSFAEIFVKRFRIRFPQLLQFDISDLYFFDSELF
                     IARNAQINIGDDIDEIEMELHRVPLIESRTE"
BASE COUNT          646 a          532 c          439 g          819 t
ORIGIN      
        1 atttgcgctg ctttttgttt cagtgcagca tcatcaccgc ctacggctct aaactccctc
       61 tgcaactgct tgagcgcatt ctcaagattc tttatgtttt gtcctgctac ttctccattg
      121 atgataatgc gcatgtctgt cttcatagac tcgcgcagct tctgcacctc tgcctgattt
      181 ttttcaagca tctcatacag tgcatcccgc ttacccggct caccggttgc agcagcatct
      241 attgcctttt ctaatttacg ggcttctgtt tcgagctcgc gtattctttt ctgcgcctgc
      301 tctgtgctta atcgtatctc tacttctccg gtttcttttc ttgtggccat gtgttttggt
      361 tacagattta tggttatttc atttttttgc atcatgtttt ctagcgtagt gatggctttg
      421 atattaaagt tatcggcaag aagttccagc aagcgtttgg tttctgctgt aatgggtttg
      481 ttatataccg gtattttctt ccttttgtat ttatgcaact gccccaaccg gtttcttttt
      541 ttataaaagt tcttgttcac aaatctgctg cctgatgcta cgcctcgccc tacacccata
      601 tcaagatagc gcagatactt atagaaacgc aagtgtattc tacttacttt atctccatgt
      661 gcatctatgc tttttacaaa ggagttatac aatcgcctcc tttcactgcg tgtgtacttc
      721 tccagtaatt cctgttcgtt ttcaacaaca tattgcagcc attgcgtaac aagctgcagt
      781 tcggtgattg gctcggctcc catgttttta tgttactatt cggttcgtga ttcaatgagc
      841 ggcactcggt gcagctccat ttctatttcg tcaatatcat caccaatatt tatctgtgcg
      901 tttcgcgcta tgaataattc cgaatcaaaa aagtacaaat cagaaatatc aaattgcagt
      961 aactgtggaa acctaatcct gaatcttttt acaaaaattt cggcaaaaga cagaatataa
     1021 acaaggtttt gccagtaata aagcaggtta gatttattcc ataccagatt atgttgcagg
     1081 tccatgccat aagtagtgat acaaacactg ctggctgtgg gtataggtcc cagatcacaa
     1141 aatcccgata ctatacctaa gtaatgtacc agatgcaaat catacgggtt atcacctagt
     1201 ccaaactctt tgctgctgcc taattgcttt atgcgtggca gaatctgata gtgtggtgtt
     1261 cccgaatcat aataaactct gcgtaatgta aaaagtggcg aaagctgcgg acgaatttca
     1321 actgcacctt tttcgtttac aacaaaatct ttatacccct cacaatagta ttgccatttg
     1381 taaacactgc ttccaaggtc attgcatttc caaatctgat gcaggcattt tacgtaagca
     1441 aaatatccta atagtgttgc gtcattatta ataagtacat caacattatc tgcctctccc
     1501 caaaatttat taatgtcgta ttctttccac gagtcggcag aagagctgtc attaccacca
     1561 aatgtgtaat taaaagattt aataacaata gaattgtcaa atttaatttc atgattggaa
     1621 gtggcctgat tgctgaaatc acgttgtggt tgttgcaaca gctcatcgaa atagtgtatc
     1681 cttactattt tgtttaccct gtcaatctta taggtgagat tgtaagtttc ttttattgca
     1741 ttgagtagct gtccgaatgt aatgtccgga acctgatcgc gcatattcca gtagcgtttg
     1801 aatacgttaa agccggtagt attaggcaaa tcgttaacac caaaataaga ataatccaat
     1861 ctgaaataac ctgatggatt tgtaccaccg ggcatcacta tttcaaatgt tagccatagg
     1921 tctttgccta catctgcagc atttgcagta aaccaccaag aggcattcca aacacttaat
     1981 gatcctgcct gactgagttg attgcttaaa aaagtatgaa taactactcc gtcctcatac
     2041 aattttagtt tataagtagg ataaacacct ggcgaactca tgttgaatcc tctgatattg
     2101 aggttaactt ttatgaggtg ttgtcctgtt gcctgaatgg tataatgtcc tgtcgtagga
     2161 ttgtatgcac ccaaactatc ataacaacca ccagttgaat cattattgaa tatgagtgta
     2221 tgttcgccca tagccgctgt atgtgttgtc aatggccatg tcgggattgt ccctgcaagc
     2281 atcccaaatc ctgtgaggct tcctttctca tctattgatt gattggtata tacaagcaaa
     2341 tttttctttg tagcatccgc cataaaacct accgtgtcat ccacactata tccgtcaaat
     2401 gcaaaaccgc ttttgatgat gtgatgcaga taaacc
//
LOCUS       DAOOOL010000260         2434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5386_length_2434_cov_50.610340, whole genome shotgun sequence.
ACCESSION   DAOOOL010000260 DAOOOL010000000
VERSION     DAOOOL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5386_length_2434_cov_50.610340"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKU82_12415"
     CDS             <1..895
                     /locus_tag="PKU82_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HOZ83712.1"
                     /translation="YKASPSEFEATALLKNERLIFNATIQQDAKADFDQSKTNQHLQV
                     SILPSNTEAYSLQEIIYEHIPYQYYFKPSEVKIVPLQLQTSSSKIGYIEGAGDEVMQC
                     LQNAGYNISVLKEENLSNDNLSNYSVIIAGVRAYNTNDWLMGYKNKLMDFVSSGGTYI
                     VQYNTNNNLGKLDNNIGPYPFKISRKRVTDEYAEMRFSEANSIVLNKPNKINADDFKG
                     WVQERGIYFAEEIDPKYQTPLSCNDKGESPLNGSLIIAQSGKGHFVYTGLAFFRQLPA
                     GVSGAYRLFSNLIALPSGQVK"
     gene            943..1617
                     /gene="trmB"
                     /locus_tag="PKU82_12420"
     CDS             943..1617
                     /gene="trmB"
                     /locus_tag="PKU82_12420"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOZ83713.1"
                     /translation="MGKDKLKRFAEMQTFERVIQPEINYHSPDFELKGKWNETIFQKQ
                     QPIVLEIGCGKGEYTVALAQRYRQKNFIGIDIKGARIWRGAKTINEEKIMNAAFLRIR
                     METIEKFFAANEVSEVWITFPDPQPKESKENKRLTSPLFLKRYSTFLQPDGIVHLKTD
                     NKDLYEFTLQTVNDAGHIILHQTTDLYKELLMPVDMDIQTTYEKKYLAAKQPIFYLSF
                     RLKKEI"
     gene            1859..>2434
                     /locus_tag="PKU82_12425"
     CDS             1859..>2434
                     /locus_tag="PKU82_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83714.1"
                     /translation="MIGKSPIQDQKNLFQPLLKEFIDMNHQLVLLSEEINWKELEADF
                     SALYSNTGLFVVAAVGGTHTELKGNDFYDHGNAFRYSVSATTADQDLQGNVWNVPTPP
                     AGFRLALNENPFYNPVFTWNDVGPPGTNTLLPHNNFQDPDDWFQPAAGTNYECATDQF
                     ACASRSFRNVATDFDYLVATDQIDNDPYTDET"
BASE COUNT          856 a          433 c          459 g          686 t
ORIGIN      
        1 atataaagcc agtccatcag aatttgaagc tacagcacta ttaaaaaacg aacgactgat
       61 ttttaatgct actattcagc aggatgcaaa agctgatttt gaccaaagca aaacaaatca
      121 gcatttacaa gtcagtattt tgccatcaaa tacagaagca tattctttgc aggaaatcat
      181 ttacgagcat atcccctacc aatattattt caaaccttcg gaagtaaaga ttgtgccact
      241 gcaactacaa acatcatcct ccaaaattgg atacattgaa ggtgccggtg atgaagtgat
      301 gcaatgcctt cagaatgcag gatataatat ttctgtttta aaagaagaaa atcttagtaa
      361 cgacaatctg tcaaattaca gtgtgattat tgcaggtgta agagcttata acaccaacga
      421 ctggcttatg ggttataaaa acaaactcat ggactttgtt tcctcaggag gtacatatat
      481 tgttcaatac aacactaaca ataatttagg taaacttgat aacaacattg gtccctaccc
      541 atttaaaatt tcacgaaaaa gggtaacgga tgaatatgca gaaatgcgat tttcagaagc
      601 aaattccatt gtattgaaca aacccaataa aataaatgca gacgatttta aaggctgggt
      661 tcaggagcgt ggaatatatt ttgcagaaga gattgaccca aagtatcaga cacctttaag
      721 ttgcaatgac aaaggagaaa gcccattgaa tggaagtctg ataattgcac aatcaggcaa
      781 aggacatttt gtctatacag ggcttgcttt tttcaggcaa ttgccggcag gtgtgtccgg
      841 tgcatatcgt ttattttcaa atttaattgc attgccatcc ggtcaggtaa aataatttaa
      901 caacttcgta aataccatta ccgttagtcg ggcattaaga ttatggggaa agacaaatta
      961 aaacgttttg ctgaaatgca gacttttgag agagtaattc aaccggaaat aaactaccat
     1021 tcaccggatt ttgagttaaa aggcaagtgg aacgaaacaa tttttcaaaa acaacaaccc
     1081 atagtcttag aaattgggtg tggcaaaggt gaatacactg ttgctttggc gcaacgctat
     1141 cggcaaaaaa actttattgg tattgacata aaaggtgcaa gaatatggcg tggcgcaaaa
     1201 accatcaacg aagaaaagat aatgaatgct gcttttctgc gaataagaat ggaaacaatt
     1261 gaaaagtttt ttgcagctaa tgaggtaagt gaagtttgga taacctttcc cgacccacag
     1321 ccaaaggagt cgaaagaaaa caaaagatta acctctcccc tttttctaaa aagatacagc
     1381 acttttttgc agcctgatgg aattgtacat ttaaaaacag ataataaaga tttgtatgaa
     1441 tttaccttgc aaactgtaaa tgatgccggt catataatat tacatcaaac aacagattta
     1501 tataaagaat tactgatgcc cgttgacatg gacattcaaa ccacctatga aaaaaaatat
     1561 ttagccgcta agcagcctat tttttatctt tcgttcaggc taaaaaaaga aatttaaatc
     1621 ccctgacttg tccaccttca aagacaactt tttgtaaatc cttgataata gtgtatttat
     1681 aaagaatttt ttgcattttg ctgacctact ggtgtggcat gtacaaaata atagaagtta
     1741 attagttaat ccttaacaac cccttccgtg tctctataaa aagttaaaaa ctttagaaaa
     1801 agaggtgata aaaactgcaa caaagtgggt tatttttgta aaaaaaggtt gcagaaaaat
     1861 gataggcaaa tcacccatac aagatcagaa aaatttattt cagccactgc tcaaagagtt
     1921 tatagatatg aaccaccagt tggttttact atcagaagaa ataaactgga aagagctgga
     1981 agcagatttt tcagcacttt attccaacac cggactgttt gtggtggcgg ctgttggcgg
     2041 aacccataca gaactgaaag ggaatgattt ttacgaccac gggaatgcat tcaggtatag
     2101 tgtatcggca actactgctg accaggattt acaaggtaat gtatggaatg tgccaacccc
     2161 acctgcgggt ttcaggttag cattgaatga gaatccgttt tataatccag tttttacttg
     2221 gaatgatgtt gggccacccg gtacaaatac cttattacct cataataatt ttcaagaccc
     2281 tgatgattgg tttcaacctg ctgctggaac aaattatgag tgcgccactg accaatttgc
     2341 atgtgcatca aggtcgttta ggaatgtggc aaccgatttt gattatttgg tagcaaccga
     2401 ccaaattgac aatgaccctt acacggatga aaca
//
LOCUS       DAOOOL010000261         2418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5437_length_2418_cov_40.041473, whole genome shotgun sequence.
ACCESSION   DAOOOL010000261 DAOOOL010000000
VERSION     DAOOOL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_2418_cov_40.041473"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1250)
                     /locus_tag="PKU82_12430"
     CDS             complement(9..1250)
                     /locus_tag="PKU82_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOZ83715.1"
                     /translation="MKIFRSGWIYFVATFLFMAVGNIALAQGNKRELEKKRERLQREI
                     NETNKLLKLTEKNKNATQAQLDALRKKISLRNDLINSINVEIKSLNGEIDKTGKSIDS
                     LERQMKQLREDYAAMIQFAQRNRNKYQTMMFVFAADDFNQAYKRLKFLKQFTENRRNQ
                     VEQIAEVQTSLNSKKADLQQKKNEKTGLKQTQESQRKNLESEKQEQDKMMENLQASEK
                     SLRKKLEAKIRDKAKLDKAIAAMVRKEIEAAKKKAVKAGKKNVTNENVFTLTPEAQQL
                     SNSFANNKGSLPWPVEKGRISETFGEHQHPLLKNVTTKNDGIDIRTSKGSSVRSIFSG
                     EVTGTVNLPNGSAVIIRHGEFLSVYSNLDEVFVTKGEKVTTKQNIGRVRTDSESGNAD
                     MNLQIWKGFTKQNPALWISKK"
     gene            complement(1327..2163)
                     /locus_tag="PKU82_12435"
     CDS             complement(1327..2163)
                     /locus_tag="PKU82_12435"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOZ83716.1"
                     /translation="MSKFLQIILCVLIPMLVFNACKVTKKSEKPIVIKPITKLEEQGA
                     ETLLKNIIDSAFFGRWISGKATVNTNIDGDKNNFNVSLRICRDSAIWISVSPLLGIEA
                     VRILLTPDTVRFLDRINKEYKVSGYKEINDLLYAGNLDFDIIQGILTGSLFVYKRNKF
                     HSVYLEDQYYILSTLSKRKLKRSLEEKDPSKPVIQDFYVDGSNYRIMKLAIEDNRIQK
                     SLETKYSGYSYTSAGYFPHHSVTNIKAEKNILVDLEYTKVTVEDTLEFPFSIPKSFKK
                     AR"
     gene            complement(2156..>2418)
                     /locus_tag="PKU82_12440"
     CDS             complement(2156..>2418)
                     /locus_tag="PKU82_12440"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOZ83717.1"
                     /translation="PNQSSFLDTYGWILYQEGKYDEAKKWIEKAMENGGENSGTILEH
                     YGDILFKLNQSDKAFEYWKKAKQSGDASGLLDQKIRDKKLYE"
BASE COUNT          637 a          494 c          395 g          892 t
ORIGIN      
        1 tcgttaaatt atttttttga aatccacaat gcaggatttt gttttgtaaa tcctttccat
       61 atctgaagat tcatatcagc attacctgat tcagaatcag tccttacacg accaatgttt
      121 tgttttgttg taaccttctc acctttagtg acaaacactt cgtcaagatt ggaataaaca
      181 gataaaaatt cgccatgcct gataattact gccgagccat tcggcaaatt gactgtgcct
      241 gtaacttctc cactaaaaat acttcgtact gagctgccct ttgatgtacg gatatcaatt
      301 ccatcattct tagttgtcac attttttaat aatgggtgct gatgttcgcc aaacgtctca
      361 gatattctcc ctttttcaac aggccatggc aaactgccct tgttattggc aaagctgttg
      421 cttaattgct gcgcttccgg tgttagtgta aatacattct cattagtaac gttcttctta
      481 cctgctttca ctgctttttt ctttgctgcc tcaatttctt ttctcaccat tgcagcaata
      541 gccttatcta actttgcttt atctctgatt tttgcttcaa gctttttgcg taatgatttt
      601 tcgcttgcct gcaggttttc catcatttta tcctgctcct gcttttcact ttcaagattt
      661 ttcctttggc tttcctgtgt ttgtttgagt cccgtctttt catttttctt ttgctgcagg
      721 tcagcctttt tactgttcag gctggtctgg acttctgcaa tctgttcaac ctgatttcgt
      781 ctgttctcag taaactgctt taaaaattta agtcgcttgt aagcctggtt aaaatcgtca
      841 gcagcaaaaa caaacatcat ggtctgatat ttatttctgt tgcgttgtgc aaactgaatc
      901 attgctgcat aatcttcgcg caattgcttc atttgtcttt caagagaatc tatagatttt
      961 cctgttttat caatctcacc attgagcgat ttaatttcaa cgttaattga gttgatgagg
     1021 tcattacgga gagatatttt ttttctcaag gcatcaagtt gtgcctgtgt ggcattttta
     1081 tttttctcgg taagtttcag cagcttgtta gtctcattaa tttcacgctg aaggcgctct
     1141 cttttcttct ccagttcgcg cttgttacct tgtgctaagg caatattgcc aacagccata
     1201 aacaagaatg tagcaacaaa ataaatccac ccggaacgga aaattttcat tgcagcaaaa
     1261 ttaggtttag tgttcccttt agtctttaag atgcaaatta attattgaca catttatgca
     1321 cgacaatcag cgcgctttct taaagctttt aggtatgctg aatggaaatt ccaatgtgtc
     1381 ttcaacagtt actttagtat attccaagtc aacaagaata tttttttctg ctttaatatt
     1441 ggttacagaa tgatgaggaa aatatccggc agaggtatat gaataaccgg aatattttgt
     1501 ctcaagcgat ttttgtatcc gattgtcttc aatggctaac ttcataattc tatagttgct
     1561 tccatcaaca taaaaatcct gaatgacagg tttactcggg tctttctctt caagcgatcg
     1621 ttttaattta cgcttgctga gtgtactgag aatgtaatac tgatcttcaa gataaacaga
     1681 gtgaaattta tttcttttat aaacaaaaag acttcctgtc agaataccct ggataatatc
     1741 aaagtcgagg ttgcctgcat aaagcaaatc gttaatttct ttgtagccgg aaactttata
     1801 ctctttattg attctgtcga gaaaacgaac tgtatcaggt gtcagcaata tgcgaacggc
     1861 ttcaatgccg agcagtggcg aaacagaaat ccagatagca ctgtcgcggc atatacgcag
     1921 cgaaacatta aagttatttt tatcgccatc aatgtttgta tttacagttg ccttaccgct
     1981 aatccatctt ccaaaaaaag cactgtcaat aatatttttc aataaagttt cagctccttg
     2041 ctcctcaagt tttgttatgg gtttaataac aattggcttc tctgatttct tggtaacctt
     2101 acaggcatta aataccagca ttggtatcaa cacacataaa attatctgta gaaatttact
     2161 catacagttt tttatccctg attttctggt ctaataatcc tgatgcatct cccgattgct
     2221 ttgccttttt ccagtattca aatgctttat cggattgatt cagcttaaat aaaatgtcac
     2281 cgtaatgttc cagaatagta ccgctgtttt cacctccgtt ttccatagct ttttcaatcc
     2341 atttttttgc ttcatcatac tttccttcct gatacaatat ccatccgtag gtatctaaaa
     2401 atgatgactg attgggtt
//
LOCUS       DAOOOL010000262         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5461_length_2411_cov_57.912139, whole genome shotgun sequence.
ACCESSION   DAOOOL010000262 DAOOOL010000000
VERSION     DAOOOL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5461_length_2411_cov_57.912139"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1551)
                     /locus_tag="PKU82_12445"
     CDS             complement(28..1551)
                     /locus_tag="PKU82_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOZ83718.1"
                     /translation="MKKLLGLFLLTVLTTFQTFAQGDFLKDSLDNYINREMQRWQIPG
                     VAIAIVKDGKVITTKGYGLRETGKPDKVDEFTLFQIASNTKAFTGTALALLDYQKRIS
                     LDDKVTKYLPWFQLKDPCATEMVTIKDILCHRIGFYTFQSDFLNWDCNMTTDALIKNM
                     RNVQPVNEFRAKYGYCNMGYVTAGEVIKAVTDTSWQDFFDYHFFKPLQMNRSSVFHQK
                     IVTDANAAKPYTLVDNKLVLLDYANIDNIGACASINSCVNDISHWIMMQLNKGKYENK
                     EVIPSAVINTTQNSYMIVRETGSARMPSNHFQNYGLGWKSEDMYGYKIVSHDGGANGF
                     VTNTTLLPELNAGFVILTNTDANWFYGALRQQLINYFANQAYTNVSENYYGIYSKNNI
                     TESEEIQNLKKQANAGVRLNVADVALVGKYSNEVYGEIEIKKEKSGLTMCFSHHPKLR
                     GALKPLSEKSLLCTFNDPTYGIHSFPFEVVNGKVNNITVKVNDFIDYQYYKFTKQNN"
     gene            1754..>2411
                     /locus_tag="PKU82_12450"
     CDS             1754..>2411
                     /locus_tag="PKU82_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOZ83719.1"
                     /translation="MFNTEKYKKICEELLPYKANLIAVTKTKPVTDIEAAIQNGQMLF
                     GENYVQELVEKVPVLPKNIQWHFIGNLQRNKVKQIAPFIHTVQTVDSRKLLNELNKHA
                     ASNNRVIKVLLQVHIAKEEAKFGFSEVEVQMLVDEGVFEQYNNISFNGLMGMATNTDD
                     TMQVRSEFKQLYGLYKSLSDAFDRNESPVLSMGMTSDYKIALDEGSNMIRIGSALFGS
                     R"
BASE COUNT          761 a          402 c          435 g          813 t
ORIGIN      
        1 aaatggtaaa cttacctttt atgtacttta attattttgt ttggtaaact tgtaatactg
       61 atagtcaata aaatcattta ctttaactgt gatattattt accttcccat taacaacttc
      121 aaagggaaaa gagtgaatac cataggtagg gtcgttaaaa gtacagagta atgacttttc
      181 agagagtggt ttaagtgcac cacgtaattt tggatgatgt gaaaaacaca ttgtaaggcc
      241 tgatttttct tttttgattt ctatttctcc atatacctca ttggagtatt ttccaaccaa
      301 tgcaacatct gcaacattaa gacgaactcc tgcattggcc tgctttttta aattctgtat
      361 ttcttccgac tcagtaatgt tgttttttga ataaatgccg tagtagtttt cgctaacatt
      421 tgtgtatgct tgatttgcaa aataattaat cagttgctga cgtaatgctc cgtaaaacca
      481 attagcatca gtattagtca gaatcacaaa acctgcattc agttcaggta ataaagtggt
      541 gttggtcaca aagccattgg caccgccatc atgcgataca attttgtaac catacatatc
      601 ttctgacttc catccaaggc cgtaattttg aaagtgattt gaaggcatac gtgcagagcc
      661 cgtctctctt acaatcatat aagaattttg agtagtgtta atcactgcag aaggaatcac
      721 ttctttgttt tcatatttac ctttgtttaa ttgcatcatt atccaatgtg agatatcgtt
      781 aacgcatgag tttattgatg cgcaggcacc aatgttgtca atgtttgcat agtccaataa
      841 aactaatttg ttgtcaacta gtgtatatgg ttttgccgca ttcgcatctg ttacaatttt
      901 ttgatgaaat acactgctgc gattcatttg taacggttta aaaaaatgat aatcgaaaaa
      961 atcctgccat gaagtatcag taactgcttt gataacctcg cctgctgtta catagcccat
     1021 attacaatat ccatacttgg cacgaaactc atttacgggc tgaacatttc tcatattttt
     1081 tattaatgca tcagttgtca tattgcaatc ccaattcaaa aaatcacttt gaaaggtata
     1141 aaaaccaatt ctatggcaga ggatatcttt aattgtaacc atttctgttg cacatggatc
     1201 tttaagttga aaccagggga gatatttagt tactttatca tctaatgaaa tccgcttttg
     1261 ataatctaag agtgccaatg cagttcctgt aaaagccttc gtattgctgg caatttgaaa
     1321 taaagtaaac tcatctacct tgtccggttt ccctgtttct ctcaatccat agcctttcgt
     1381 ggtgattacc tttccatctt ttacaatggc aatggcaaca cctggtattt gccatcgttg
     1441 catttcacga ttaatgtagt tgtcaagact gtcttttaaa aaatcgcctt gtgcaaatgt
     1501 ttgaaaggtg gttaacactg ttaacagaaa aagacctaag agttttttca tttcgataaa
     1561 tttaatgtat caaatgtatg gaattttaaa tatacaagca cctgcattgt agtttatccg
     1621 gaataccggt tttactttct gagagcaagc tatgccaatg ggtgtggctt gcttattttt
     1681 gtagaaagat ttttctaaac tataaatgag tctcagataa attacgaatt ttaccgttga
     1741 ttatctgact cagatgttta atacagagaa atacaaaaaa atatgtgaag agttgttgcc
     1801 ttataaagcc aaccttattg cagttaccaa aactaaacct gttacagata ttgaggctgc
     1861 cattcaaaat ggacaaatgc tttttggcga gaattatgtg caggaactgg tggagaaggt
     1921 gcctgtctta ccgaaaaata ttcaatggca ttttatcggc aatctacaac gcaataaagt
     1981 aaaacagatt gcaccattta ttcatactgt acaaacggtt gatagtagaa aacttttaaa
     2041 tgaattaaat aaacatgctg catcaaataa cagagtaatt aaagtgcttt tacaggtaca
     2101 tattgcaaaa gaagaagcaa agtttgggtt ctctgaagtt gaagtgcaaa tgttggttga
     2161 tgaaggggtg tttgaacaat acaacaatat tagttttaat ggattgatgg ggatggcaac
     2221 taataccgat gatactatgc aagtgcgttc tgagttcaag cagttgtatg gattgtataa
     2281 atcactttcc gatgcatttg acagaaatga gtctccggta ttaagtatgg gcatgacatc
     2341 cgattataaa attgcattag atgaaggaag taatatgatt cgaataggtt cagcattgtt
     2401 tggaagcaga t
//
LOCUS       DAOOOL010000263         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5601_length_2367_cov_73.076125, whole genome shotgun sequence.
ACCESSION   DAOOOL010000263 DAOOOL010000000
VERSION     DAOOOL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_2367_cov_73.076125"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..2068)
                     /locus_tag="PKU82_12455"
     CDS             complement(20..2068)
                     /locus_tag="PKU82_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83720.1"
                     /translation="MYIFPSQSLLADEFNAVKANIAQSCLTPAAAQEILNLTPSTDRN
                     YIELQLGRTEEMRRLLADAEPFPVSGYCDVTKELQLLKIENSVLLPSQALQLAALTHT
                     VGLIIKFFNQAPRYPLLTQILNDCFFEPAIASEVERVLDTFGVVRSSASTELLHIRRT
                     LQRQRAESDRAYQQVIARYKKNGWLTDSEESSRNGRRVLSIVAEQKRTINGVIHDVSA
                     TGKTAFVEPDEVLHINNHIQQLEQDERLEITRIMRQLTVFLRNYYTTLQQYAFILTQM
                     DMHHAKALFALNYRAVKPEITNNPEINLINASHPILLIQQKDIQKSVVPFTLHLNHDK
                     RILIISGPNAGGKTVCMKAVGLLLIMAQSGIHITAKTGTTVGVFNKLLIDIGDSQSIE
                     YELSTYSSRLKNMKVFFEQVDNKSLFLIDELGSGTDPQLGGALAEAMLETLNQNHSFG
                     IVTTHYMNLKVLAEKVPGFVNGSMSFDKRNLKPLFQLQIGKPGSSYTFIVAERSGLPQ
                     QIINSARAKVPKSHMLLEKLLSKVEKDKIVIEEKLKAILQKEKTLNTLIKQNDELIYK
                     NENLKNSLQQHLRSHQENLDRKAEARIKKFALDLKQAKNKKAVIEKFLSEAGLKNKNK
                     EKVVHRTIDPRIISGTTVKLYNGKVSGIVQSVDQENATVLFGNFTTKCKLTDLILDSK
                     "
BASE COUNT          680 a          444 c          449 g          794 t
ORIGIN      
        1 ttttactaca gtatgatatt tatttactat caagtatcag gtcggttaac ttgcactttg
       61 tagtgaagtt tccaaataga acggttgcat tttcctgatc aacagattga acaattcctg
      121 atacttttcc attataaagt ttcacagtag tgccactaat aatacgtgga tcaatggttc
      181 tgtgaacgac cttctcctta ttcttgtttt taagccctgc ttctgaaaga aatttttcaa
      241 ttacagcctt tttattcttt gcctgtttaa gatctaatgc aaacttctta atacgtgctt
      301 cagcctttct atcaagattt tcctgatgac tgcgcaaatg ctgctgaagg ctattcttga
      361 gattttcgtt tttataaatt agttcatcat tctgttttat gagcgtgtta agtgtctttt
      421 ctttttggag aattgctttt aacttttctt caatcactat tttgtctttt tcaacttttg
      481 atagtagttt ttcaagtagc atgtgtgact tcggcacctt tgcacgagca ctgttgatga
      541 tttgttgcgg tagcccgctt cgttctgcca caataaatgt ataagagctg ccaggcttac
      601 caatttgcag ttgaaagagt ggttttagat tacgtttgtc aaacgacata gagccattaa
      661 cgaatccagg cactttctct gcaagtactt tcaggttcat ataatgcgta gttacaattc
      721 caaatgagtg gttttggttt aatgtttcaa gcattgcttc agccaatgca cctccaagtt
      781 gagggtctgt accactacca agttcatcaa tcagaaagag tgatttattg tcaacctgct
      841 caaaaaacac tttcatgttt tttaatcggg aactgtatgt gcttaattcg tattcgatag
      901 attgagaatc gcctatatca ataagaagtt tattgaaaac accaactgta gttcctgttt
      961 ttgcagtgat atgtattcct gactgcgcca tgataagcaa aagcccaacg gctttcatgc
     1021 atactgtttt acctccggca ttaggtccgc tgatgattaa tattctcttg tcgtgattca
     1081 gatgaagcgt aaatggaaca acactttttt gaatatcctt ttgctgaatt aacaatatcg
     1141 gatgcgaagc attgataaga tttatctcag ggttattcgt gatttcaggt tttacggcac
     1201 gatagttcag tgcaaaaagt gctttggcat gatgcatatc catttgagtg aggataaatg
     1261 catattgctg aagtgtggta taataattac gcaggaatac agtaagttgt cgcatgattc
     1321 gagtgatttc aagtctctca tcctgctcta actgttgaat atgattattt atgtgcaaca
     1381 cttcatcagg ctcaacgaat gcagtttttc ctgttgcaga aacatcatgg atgacaccat
     1441 tgattgtccg cttttgttct gcaacgattg aaagaacccg tcttccgttg cgtgatgact
     1501 cttcgctatc tgtaagccat ccatttttct tatagcgtgc aatgacttgc tgataggctc
     1561 tgtctgattc agcacgttgc ctttgtaaag tacgtctgat gtgcaagagt tccgttgatg
     1621 cagaagaacg caccacacca aaagtgtcaa gcactctctc aacttcactt gcaatggcag
     1681 gttcaaaaaa gcaatcattg agtatctgtg tgagtaaagg atatcgtggt gcctgattga
     1741 aaaatttaat aattagacct accgtatgtg tgagtgcagc aagttgcaat gcttgtgaag
     1801 gcagcaatac gctgttttca atcttaagca gttgaagttc tttcgtaaca tcacaatagc
     1861 ccgaaacagg aaaaggttct gcatcagcaa gcagcctacg catttcttct gttctcccaa
     1921 gttgtaattc aatataattt ctatcagtac ttggagtcag attaagaatt tcttgtgcag
     1981 cagcaggtgt aagacaactc tgggcaatgt ttgcttttac ggcattgaac tcatccgcaa
     2041 gtaacgactg agatgggaaa atatacatgt aaacaattca aaataaattt tcagttttat
     2101 gcataaactt aaggtatgcc caatactgaa gtgcgaaatt aatacatggc catgatacca
     2161 aacaaattat taaatttata tgagataatt atcaggcgac tgactgtata gaaacaagtc
     2221 tgttctgtct gacattttga atgcatgaaa tcaaagccgg aatagcaatg agtaaaatgc
     2281 ctgcaaaaat gccaattcct gcaatgaaaa agctataaaa atacggtagc attttttgca
     2341 tagctgatgc aaatgttccc ccattac
//
LOCUS       DAOOOL010000264         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5664_length_2351_cov_110.998258, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000264 DAOOOL010000000
VERSION     DAOOOL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_2351_cov_110.998258"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..365
                     /locus_tag="PKU82_12460"
     CDS             60..365
                     /locus_tag="PKU82_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83721.1"
                     /translation="MRQMYDHAAEARKAVSEQRTVSYPKTFLNINTAKPTDDKTKNEY
                     YTTFADLYLQTLDSYENSTNTNRVKSFNNVVNACLACHSSHCPGPVPKIKRLLIPLD"
     gene            367..1122
                     /locus_tag="PKU82_12465"
     CDS             367..1122
                     /locus_tag="PKU82_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83722.1"
                     /translation="MQVRVFLFAILISFSSLQSAAQALVRDSAIIIPFFGIGGEFHLP
                     GGDMKERFGADYGVQGVFGVKTPKNFLYSIQGGFFFGNKINQPGLLAGIASSYGTIIG
                     SDGRTADVRLFERGYHISAQAGKVFAFKKPNPNSGIWISAGAGFMQHKIRIETIGNTV
                     KGLSKKYKKGYDRLTNGIIVEEFVGYVYYGNKKLINFYAGFNFIQGFTQGRRDYQFDT
                     MKADDQSRVDLLYGFKAGWILPFYTNPDKFYYY"
     gene            1125..>2351
                     /locus_tag="PKU82_12470"
     CDS             1125..>2351
                     /locus_tag="PKU82_12470"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOZ83723.1"
                     /translation="MLLLYSIGIRLYHLAAALASPFNDKAKAFTEGRKGLFQRVQQEL
                     KNETAKHIWFHCSSYGEYEQGKPVLQQIKKQFPEYKIALTFFSPSGYKQGIKENLADY
                     IFYLPCDSSNNARQWLDYINPHMAIFVKYDFWFHYMKELNKRSIPFIYISAIFRIDQF
                     FFKWYGGAFLKELKKATRFFVQNKSSLNILKQEGITQVEVSGDTRFDRVIQLPELAFT
                     DEKIESFIAEKRVIVAGSTWPEDHKLLAAYCNSEEIKLILVPHEIHESSFKDFVSACK
                     KNVMCYSKASQEELIKADVLIIDKIGMLSKIYRFATITYIGGGFGKGIHNTLEAAVYG
                     KPVIFGPNYQKFEEAKQLIESGGAFTINNFIEMKTVFDDLLNNNRKLAQAGEISQNVV
                     KSNAGATSIIANWLQNQ"
BASE COUNT          789 a          370 c          459 g          733 t
ORIGIN      
        1 ttggttttga agccaattgg caataataga tgttgcgcct gcattagatt tcactgctga
       61 tgcggcagat gtatgaccat gctgctgaag cacgaaaggc tgttagcgag caacgtactg
      121 tttcatatcc aaaaacattt ttaaacatca atacggcaaa gccaactgat gataaaacta
      181 agaacgagta ttatactacg tttgccgact tatatcttca gactttggat tcgtatgaaa
      241 attcaacaaa cacaaacaga gttaagagtt tcaataatgt tgtgaatgca tgtctggctt
      301 gtcatagcag tcactgccca ggacctgtac caaaaattaa acgcctttta attccacttg
      361 attaaaatgc aggttagagt ttttttattt gccatactga tttctttttc ttcattacaa
      421 tctgctgcac aggctttggt gcgtgattca gctattatca ttccattttt tggaatagga
      481 ggcgaatttc atttacccgg tggtgatatg aaagagcgtt ttggcgcaga ctatggagtg
      541 caaggtgttt ttggtgttaa gacaccaaag aattttcttt acagtattca aggcggattt
      601 tttttcggaa ataaaatcaa tcaaccagga ttacttgcag gtattgcatc aagttatgga
      661 acaataatag gcagcgatgg ccgcactgca gatgtaagac tttttgaaag aggatatcac
      721 atcagtgcac aagccggaaa agtgtttgca tttaaaaaac ctaaccctaa tagtggtatt
      781 tggattagtg caggtgcagg atttatgcaa cataaaatca gaatagaaac cataggaaac
      841 acagtgaagg ggctttctaa aaaatacaaa aaaggttatg acagactgac caatggaatt
      901 attgttgaag aatttgtcgg ttatgtttat tatggtaata aaaagctgat aaacttttat
      961 gcaggcttta attttattca gggatttaca caaggacgca gagattatca gttcgatacc
     1021 atgaaagccg atgatcaaag ccgtgttgat ttgttatatg gttttaaagc cggttggata
     1081 cttcctttct ataccaaccc cgataaattt tattactact aatcatgttg ttgctttatt
     1141 ccattggaat aagactatat catttggcag cagcacttgc ttcgcctttt aatgataaag
     1201 caaaggcttt tactgaagga cgaaaaggtt tatttcaaag agtacaacaa gaactaaaaa
     1261 atgaaactgc aaaacacatt tggtttcatt gttcttcgta tggcgaatat gagcaaggca
     1321 aacctgtttt gcaacaaata aaaaaacagt ttccagaata taaaatagca cttacttttt
     1381 tttctccatc aggctacaag caaggcatca aagaaaatct ggctgactat attttttatt
     1441 taccttgtga tagtagcaat aatgcacgtc aatggttgga ttacataaat ccacacatgg
     1501 caatatttgt aaaatatgat ttttggtttc attacatgaa agagttgaat aagagaagta
     1561 ttccatttat ttatatctct gctattttta gaattgatca gttttttttc aaatggtatg
     1621 gaggtgcttt tcttaaagag cttaaaaaag caacaaggtt ttttgttcag aataaaagct
     1681 ctctcaatat tttaaaacaa gagggaataa ctcaggttga agtgagtggc gacacacgtt
     1741 ttgacagggt aattcaacta cctgagttgg catttacgga tgaaaaaatc gaaagtttta
     1801 tagcagaaaa aagggttatt gttgccggca gcacctggcc tgaagatcat aaattgctgg
     1861 ccgcttattg taattccgaa gagataaaat taatattggt gccacatgaa atccatgaaa
     1921 gttcattcaa agattttgtt tctgcatgta aaaaaaatgt aatgtgttat agtaaggctt
     1981 cacaagaaga attaataaaa gccgatgtgt tgataataga taaaattgga atgctttcta
     2041 aaatttatcg ttttgcaaca ataacctaca ttggtggtgg ctttggaaaa ggaatacaca
     2101 acaccttgga agcggctgtg tatggcaaac ctgtaatttt tggccccaat tatcaaaagt
     2161 ttgaagaagc taaacagttg attgaatccg gaggtgcatt tacaatcaat aactttattg
     2221 aaatgaaaac tgtttttgat gatttgttaa acaacaatag aaagttggct caagcaggtg
     2281 aaatcagcca aaatgtagtg aaatctaatg caggcgcaac atctattatt gccaattggc
     2341 ttcaaaacca a
//
LOCUS       DAOOOL010000265         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5710_length_2337_cov_34.001753, whole genome shotgun sequence.
ACCESSION   DAOOOL010000265 DAOOOL010000000
VERSION     DAOOOL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5710_length_2337_cov_34.001753"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="pfkA"
                     /locus_tag="PKU82_12475"
     CDS             complement(<1..952)
                     /gene="pfkA"
                     /locus_tag="PKU82_12475"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOZ83724.1"
                     /translation="MNIQKIALLTSGGDSPGMNACIRSIVRSAISQKKKVVGIMRGYE
                     GMIHGEFVELNARSVSNILQRGGTILKTARSKAFMTPEGMKKAYDNLCREGIEGIIAI
                     GGDGTLKGAVEFNKVNNIPFIGIPGTIDNDLFGTDYTIGFDTALNTIIDAVDKIKDTA
                     ASHDRLFFVEVMGRDSGCLALYSGIASGAEEILMPEQKTDISALVEKLKESVQRRKTS
                     SVVIVAEGDDGGGAFKIAEAVKKSFDYDIKVTVLGHIQRGGSPTAKDRIMATRFGAEA
                     VNALLSGQTNKMTGWNGKEVTLIDLEKVVKQHNPFDKRLVS"
     gene            1046..>2337
                     /locus_tag="PKU82_12480"
     CDS             1046..>2337
                     /locus_tag="PKU82_12480"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOZ83725.1"
                     /translation="MTKFTLLAAFAVLFSGASIAQDRVPVNQNPAIGARSQSVIATEF
                     TNSGQLIPRNGNSAINVAFSDDFSQQNDTSSLIARGYLPYYRGTGAQGISATWFQPDG
                     TVFPAYNGAISEYVAANYNVVTATNNIDSWLVTPALNVASGDKISFYARSVTSSTWVD
                     SIRVMYSAAGDSTPEGLTWVELGRFQASVAGTWDYINYAVTTAGATARFAIRYAVIDG
                     GPNGSNSNFIGVDQLDIYTPQTTDASVFAISSMNSGCGLSATTPVDVSIFNWGGSAIS
                     GFPVSFVVDNGTPVTETFTGTIPAGGSSSFLFTGTADFSAVGPHTIKAYSSLSGDGNA
                     ANDTTTATVTNIPPIVLGSTAVTEGFEAASGTTPPPGWTIEDTDGDGNIWDFATTYTH
                     NGLVCARLGVAQAMAGTPENWLFSPCLDLTAGTNYKIE"
BASE COUNT          679 a          479 c          434 g          745 t
ORIGIN      
        1 atgacaccaa tcgcttgtcg aaaggattgt gttgtttaac tactttttca aggtcaatca
       61 aagtaacttc tttaccattc cagcctgtca ttttattggt ttgaccactg agaagtgcat
      121 ttacagcttc agcaccaaac cgtgtagcca ttattctgtc tttagcagtt ggactgcctc
      181 cacgttgaat atgaccaagc acagtaactt taatgtcgta gtcaaaagat ttttttactg
      241 cttctgcaat tttaaatgca ccgccaccat catcaccttc agcaacaatc accacacttg
      301 aagtttttct tctttgtaca ctttctttta gtttttcaac caaagcactg atgtccgttt
      361 tttgttcagg cattaatatt tcttcggcac cactggcaat tcctgaataa agtgctaagc
      421 aaccggaatc acgacccata acttccacaa aaaacaatct gtcatgcgat gctgcagtgt
      481 ctttaatttt atccacagca tcaattatag tattcaatgc ggtatcaaaa cctattgtgt
      541 aatctgttcc aaaaagatcg ttatctattg ttcctggaat accaataaat ggaatattat
      601 tgactttatt aaactcgacc gcgcctttca atgtgccatc accaccaata gctataatgc
      661 cctctattcc ttcacggcaa agattgtcat aagccttttt catgccttca ggagtcatga
      721 aagccttgct gcgtgctgtt ttaaggatag ttccacctct ttgtaaaata ttgctgacag
      781 agcgggcatt taattctaca aactcaccat gaatcattcc ttcataaccc cgcatgatgc
      841 cgactacttt tttcttttga gaaatggcgc tgcgcactat tgaacgaata caggcattca
      901 ttcccggaga atcgccaccg gaagtaagta aggcaatttt ttgaatgttc ataatttttg
      961 aaaaaagaat aggcaaattt aatccattag aatctctttt tttactttta caccattaaa
     1021 ccattataaa tttaaaaaat cacaaatgac aaaatttaca ctattagctg ctttcgcagt
     1081 gttgttttcc ggtgcttcca tagcgcaaga ccgggttcct gtgaatcaaa accctgctat
     1141 tggggcaaga tcacagtctg taatagctac agagttcaca aattcaggac aattgattcc
     1201 aagaaatgga aacagtgcca tcaatgttgc tttttctgat gacttcagtc aacagaatga
     1261 cacttcttct ttgattgcca gaggttacct tccttattac agaggtacag gtgcgcaagg
     1321 tatatctgca acatggtttc aacctgatgg aacagttttt cctgcatata atggcgctat
     1381 ctctgaatat gtagctgcca attataatgt tgtaactgca acaaataata ttgatagctg
     1441 gttggttacg cctgcattaa atgttgcatc aggcgataag atttcatttt atgcaagaag
     1501 tgttacaagt tctacatggg ttgattcaat tcgtgtaatg tactctgcag ccggtgattc
     1561 tactcctgaa ggtttaactt gggttgaatt aggaagattt caagcaagtg ttgcaggaac
     1621 gtgggactat attaattatg ccgttactac tgcaggcgct actgcacgtt ttgcaattcg
     1681 ttatgctgtt atagatggtg gtcctaatgg ttcaaacagt aattttattg gagttgacca
     1741 attagatatt tatacaccac aaacaactga tgcaagtgtg tttgcaataa gcagcatgaa
     1801 ttcaggctgt ggtttaagtg caacaacacc agtcgatgtt tcaatattca attggggtgg
     1861 aagcgcaatt tccggattcc cagtatcatt tgttgttgat aatggtacgc ctgtaactga
     1921 aacttttact ggaacaattc cggcaggagg ttcatcatca tttcttttca caggtacagc
     1981 tgatttttct gcagttggac cacatacaat aaaagcttat agcagtcttt caggagatgg
     2041 taatgcggca aatgatacaa ctactgcaac tgtaacaaat atacctccaa tagtactagg
     2101 ttctactgca gtaacagaag gttttgaagc tgcttcagga acaacaccgc ctccgggatg
     2161 gacaattgaa gacaccgatg gtgatggtaa tatatgggac tttgctacta cctataccca
     2221 taatggttta gtatgtgctc gtcttggtgt tgctcaggca atggcaggca ctcctgaaaa
     2281 ctggttattc tcaccatgtc ttgatttaac tgcaggaaca aattataaaa ttgaata
//
LOCUS       DAOOOL010000266         2335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5713_length_2335_cov_53.155263, whole genome shotgun sequence.
ACCESSION   DAOOOL010000266 DAOOOL010000000
VERSION     DAOOOL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_2335_cov_53.155263"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..294)
                     /locus_tag="PKU82_12485"
     CDS             complement(37..294)
                     /locus_tag="PKU82_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83726.1"
                     /translation="MPLRGNMTVSFRVDAVKTLLLSLSKESSEELSTYFRRLKDVIVF
                     MYNPEQKHVDWEEIQDFFLDMGIIADFFDQMEIETTGGDDE"
     gene            complement(563..1264)
                     /locus_tag="PKU82_12490"
     CDS             complement(563..1264)
                     /locus_tag="PKU82_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83727.1"
                     /translation="MALIVACGPIAKEKVDTTIDDFGIFREEPIETGYSAKITTYAFL
                     KTDTITEERLIMTLNKIYDRLEKYDKFTNFSKPTAITIYMYESSESARKYPELWLAML
                     RKSQKDSMPIFSFDKTRMSVYDKSNDPTDDVKAEIVKAIEDSIQLENLKYEKITNYFI
                     KRGITICFIYKKLYDLQGESIKKADVMFPDFGTSHSLYQEQIYKEERSKIFKKYNIND
                     TLVDYIASYGYKNCR"
     gene            complement(1676..>2335)
                     /locus_tag="PKU82_12495"
     CDS             complement(1676..>2335)
                     /locus_tag="PKU82_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83728.1"
                     /translation="GEKAVEANPEIAQLLMKSSKQGGVKIDNEEIYKIVEKYIPEAKR
                     ITTREVPDSFSTITEQVSTQHNTAHGRPDISTSGVQIENDDFASRVLSNIFHPVEQVN
                     VPRFVERSARVPQIDTQSIVDSIQVSRLGYATALPVLPEQRPTQQQKEDMLNITGESK
                     DIAATLKQLQTTMAEMKTAIDQAKQTWEKPFDVKAKVVLRDLNEARRLEETAKRNARI
                     G"
BASE COUNT          624 a          481 c          397 g          833 t
ORIGIN      
        1 atttgcttct tagcccaaat tatgttaata gattgttcat tcatcatcgc ctcctgtcgt
       61 ttcaatttcc atttggtcaa agaaatcagc aattattccc atgtcaagaa agaaatcctg
      121 aatttcttcc cagtccacgt gcttttgttc aggattgtac atgaaaacta tcacgtcctt
      181 aagcctcctg aaatacgtgc ttaattcctc gctactttcc ttactcaggc ttaaaagtag
      241 cgtttttacg gcatcaaccc taaatgatac cgtcatgttg ccgcgtagcg gcatttgtac
      301 ccgcgtttgc aggtttttct cttgtgtgtt cataatctaa gagtgttgtg cccggatgtg
      361 aacacacaag caccacgagg gtatttgagg tgagggcgtc accgcttcct ccatccggga
      421 tattgttaaa caatagtttg aaagtttgca tcgtagtgcg ttatgtgttc gctgcaaaca
      481 tacataaaaa aacaaacccc gccaaattag gcagggtatt tttttaacac aatactgttt
      541 aatgtgcagt tagtgggtat tttcagcgac agtttttgta cccataagaa gcaatgtaat
      601 ctacaagcgt atcgttaatg ttatattttt tgaaaatttt gcttctttct tccttgtaga
      661 tttgttcttg gtaaagggaa tgtgatgtgc cgaaatcagg gaacataaca tctgcctttt
      721 ttatggattc gccttgtaaa tcgtaaagtt tcttataaat aaaacaaatg gttatccctc
      781 tttttatgaa ataatttgta attttttcgt atttcaaatt ttctaattga atagaatcct
      841 caatagcttt aactatttct gcttttacat catcagtagg gtcatttgat ttgtcataaa
      901 cagacattct cgttttgtca aatgagaata ttggcatgct gtctttttgg ctttttctta
      961 gcatggcaag ccataattca ggatattttc ttgcagactc actactttca tacatgtaaa
     1021 ttgtaattgc agtaggcttg gaaaagttcg tgaatttatc atatttctct aaccgatcat
     1081 aaattttatt caatgtcatt ataagccgct cttccgttat ggtatctgtt ttaagaaaag
     1141 cataagttgt tattttagcc gaatatccgg tttcaatagg ctcttctcta aaaataccga
     1201 agtcgtcaat agttgtatcg actttttctt ttgcgattgg tccgcaagct acaattaatg
     1261 ccatcacagc cgtaattgtt attaaaataa tttgtttaat catatgtgct gaaattttaa
     1321 aaaataatat gttgcaaacc taaaaaatcc tttctatcag ccacaatata aatcctgtta
     1381 aagctaatac gataatggca ggaactgcaa ttttatcaat taagtcgtaa tgttgttttc
     1441 tcaccaaaca aagttacaca atttagttta tatgtaggtt cagaaaacat tgagattatt
     1501 gagatctcat tgagatatct cactgttctc aatgaatttc atcacgaatg aataaaaaaa
     1561 ccgcacagct tacgggctat gcggtccaac tcaatcatta tgaaaaacta aagctatgac
     1621 ttttctcctc tacgtatatt cctttcgcag ggttacattt atttttaaat tattttcacc
     1681 ctattcttgc atttcgcttc gctgtttctt caagccttct tgcttcgtta aggtcgcgca
     1741 acactacctt ggctttaacg tcaaaaggtt tttcccacgt ttgttttgcc tgatctatcg
     1801 ctgttttcat ttcagccatg gttgtttgta attgtttcag tgtggccgct atgtctttgc
     1861 tttctccagt aatgttcagc atatcttctt tctgctgctg tgttggacgc tgctctggca
     1921 acaccggcaa agctgttgca taacccaatc tgcttacctg tatgctgtca acaatgctct
     1981 gcgtgtctat ctgcggcaca cgggcactac gctccacaaa acgtggcaca ttaacttgct
     2041 ccaccggatg gaaaatatta gaaagcacac gactcgcaaa atcatcattc tctatttgaa
     2101 cccccgaagt actgatatct ggtctgccat gcgctgtatt atgttgtgtc gaaacctgct
     2161 ctgtaatggt cgagaaacta tcaggcactt cacgggtagt tattcttttc gcctcaggaa
     2221 tatacttttc tacaatttta taaatctctt cgttgtcaat tttaacaccg ccttgcttgc
     2281 tgctcttcat tagcagttgt gctatttccg gattcgcttc cactgccttt tctcc
//
LOCUS       DAOOOL010000267         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5749_length_2325_cov_26.055507, whole genome shotgun sequence.
ACCESSION   DAOOOL010000267 DAOOOL010000000
VERSION     DAOOOL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_2325_cov_26.055507"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PKU82_12500"
     CDS             <1..1070
                     /locus_tag="PKU82_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ83729.1"
                     /translation="MGANTFTIRNSGLGIRLGNSGKKAKRFKEITLQQAVLFKSGNTY
                     PSIISISTMCNGVAVCRVGSEKTNPNILVMAIDENYLQTGGYTIATGRNMTQTEAMRG
                     DNVVMIGSEIKSKLFPGADAVDKEVYIDNRKYRIIGVLAEKGNSMGFGGDKVCLISLI
                     NGKQNYGSDNRSWTVSVKVNHVSGLEAAVDEATGLFRTIRKDRVGEESSFEIIKSDSI
                     AAIVIDQLKNVSIAATLIGLITLLGAAIALMNIMLVSVTERTREIGIRKSLGATRLVI
                     LKQFLIEAIVICLLGGVAGVILGVLVGNLMGMAMNAVFVVPWNWIIAGLLLCGFIGIA
                     SGFYPAMKASRLDPIEALRYE"
     gene            1283..1651
                     /gene="rpsF"
                     /locus_tag="PKU82_12505"
     CDS             1283..1651
                     /gene="rpsF"
                     /locus_tag="PKU82_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOZ83730.1"
                     /translation="MSNQYETVFILTPVLSNEQMNEAVDKFRKILTDNGAMIVHENNW
                     GLRKLAYPIQKKNTGFYYLIEFKSSGELINKLEVEYKRDERVMRFLTVALDKHAIAYN
                     EKKRKNAELKKKEQETATAN"
     gene            1660..1932
                     /gene="rpsR"
                     /locus_tag="PKU82_12510"
     CDS             1660..1932
                     /gene="rpsR"
                     /locus_tag="PKU82_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOZ83731.1"
                     /translation="MMSSKQGEIRYLNPVAVDTTKKKYCRFKKVGIKYIDYKDATFLI
                     KFVNEQGKLLPRRITGTSLKYQRKVGQAVKRARHLALMPYVGDLLK"
     gene            1945..>2325
                     /gene="rplI"
                     /locus_tag="PKU82_12515"
     CDS             1945..>2325
                     /gene="rplI"
                     /locus_tag="PKU82_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOZ83732.1"
                     /translation="MEIILKQDVKNLGYADEIVKVKNGYGLNYLIPKGMAVIASEANK
                     KVHTETMKQRAHKMAKIKGDAEKLADAISSVTLTIPVKVGENGKLYGSVTSQNIADSL
                     KKLGYSIDKKQIEMPEEHIRKTGTY"
BASE COUNT          794 a          380 c          509 g          642 t
ORIGIN      
        1 gtatgggagc aaacacattc accataagaa attcaggttt gggaatcagg ttgggtaatt
       61 caggcaaaaa ggccaaaaga tttaaagaaa ttacacttca gcaggcagtg ttgtttaaat
      121 caggcaatac ctatccttca attattagta tttctacaat gtgtaatgga gtggcagttt
      181 gtcgagtggg ttcagaaaaa accaatccga atattttggt tatggctatt gatgaaaact
      241 atttacaaac cggaggttat acaattgcaa caggtcgtaa tatgacgcag acagaagcta
      301 tgcgtggcga taatgtagtg atgattggct ctgaaataaa gagtaaactt tttcctggtg
      361 ctgatgctgt tgacaaagaa gtttatatag acaacagaaa atatagaatt attggcgtac
      421 ttgccgagaa aggtaacagc atgggttttg gtggtgataa agtatgtttg atttcattaa
      481 tcaacggtaa gcaaaactat ggcagcgata atcgttcatg gacagttagt gttaaggtga
      541 atcatgtgtc aggccttgaa gcagcagttg atgaagcaac aggactattc agaacaataa
      601 gaaaagatcg tgttggcgaa gaaagctcat ttgaaataat aaaaagcgac agcattgcag
      661 caattgtaat tgatcagttg aagaatgtaa gtattgcagc aacgctgatt ggacttatca
      721 cattgcttgg tgcagcaatt gcattgatga atattatgct tgtctctgtc acagagcgaa
      781 cccgcgaaat aggaatcaga aaatcacttg gtgcaacaag gcttgtaatc ttaaagcagt
      841 ttttgattga agcaattgtt atttgcctct tgggtggcgt agcaggagtt attttaggtg
      901 tgttggtcgg caacctgatg ggcatggcta tgaatgctgt ttttgtggtg ccttggaact
      961 ggattattgc cggattgttg ctatgtggtt ttattggcat tgcctcaggt ttttatcctg
     1021 ccatgaaagc ttcgcgcctc gacccaattg aggctttacg ctacgaataa aatatttgat
     1081 cattctaatt catctgaatt attttattat atagttgatt ttcaaatttt taatatattt
     1141 tacttaaagt aatttattgt agcgattcgc ttaggatgag tatcttaaaa aaaagtaaaa
     1201 attctatttc aagaaattaa taataacact atctttgccg tccgcttaaa aaaggctatt
     1261 aaactgaaat atatataaac aaatgtcaaa ccagtacgaa accgtattca ttcttacacc
     1321 cgtactcagc aatgagcaga tgaatgaagc ggttgacaaa ttcaggaaaa tcctcaccga
     1381 caacggtgcc atgattgttc atgagaacaa ttggggatta cgcaagcttg cttatccgat
     1441 tcagaagaag aacacgggct tctattatct gatcgaattt aagtcaagcg gtgaactcat
     1501 caacaaattg gaagtcgaat acaaacgcga tgagcgtgtg atgcgattcc tgactgttgc
     1561 gctcgataag catgccattg cttacaatga aaagaagaga aagaatgcag agttgaagaa
     1621 gaaagaacaa gaaacagcaa ctgctaatta aacgaaggaa tcatgagctc aaaacaagga
     1681 gaaattaggt acctcaaccc ggtagcggtt gacaccacaa agaagaaata ctgccgtttc
     1741 aagaaagttg gaatcaagta tattgactat aaagatgcta cattcctgat taagtttgtt
     1801 aatgaacaag gcaaactttt accacgcaga ataaccggta catcattaaa ataccagcgt
     1861 aaagttggtc aggctgttaa acgtgcgcgc catcttgccc tgatgcctta tgttggagat
     1921 ttgttaaaat aagaggaggc aacaatggaa attatattaa aacaagatgt gaaaaatctc
     1981 ggctatgccg atgaaatcgt gaaggtaaaa aatggctacg gcctgaatta ccttatccct
     2041 aagggaatgg ctgttattgc cagcgaagcc aacaaaaaag tgcatacaga aacaatgaaa
     2101 caacgtgcgc acaagatggc aaaaatcaaa ggtgatgcag agaaattggc tgatgctatt
     2161 tcaagcgtta ccctgacaat acctgtaaag gtaggcgaaa acggcaagct ttatggttct
     2221 gtaacttctc aaaacattgc tgactcattg aagaaattgg gttatagtat tgataagaag
     2281 caaatcgaaa tgcctgaaga acacattcgt aaaacaggaa catac
//
LOCUS       DAOOOL010000268         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_5752_length_2324_cov_34.876157, whole genome shotgun sequence.
ACCESSION   DAOOOL010000268 DAOOOL010000000
VERSION     DAOOOL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5752_length_2324_cov_34.876157"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKU82_12520"
     CDS             <1..540
                     /locus_tag="PKU82_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOZ83733.1"
                     /translation="PMAVRFFMMQTHYRSTLDFSNEALQAAEKGYQRLMSAVKKLNSV
                     KASDVTSVSLSSIRKNAYDAMNDDFNSPVMVAQLFELVRIINSANDGKEKMTKEDIAE
                     AKSIIQHFVFDIAGLMDDTVSSGDQSVLDNVMQLVLEMRNQAKAKKEFSVSDHIRNSL
                     NAMGINVKDSKDGSTWEKM"
     gene            515..1525
                     /locus_tag="PKU82_12525"
     CDS             515..1525
                     /locus_tag="PKU82_12525"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOZ83734.1"
                     /translation="MAALGKKCNFLNLIAVAVLLVVGSCKQPAQESTETKQETPATVN
                     IPVPAFNADSALHYLQTQVAFGPRVPGTDAHAQCADYLFQKLKSFGLQTQIQSETITT
                     FDSKKFRLKNIIAEYKPEAKQRVLLLAHWDTRPWADHDSVRKDQPFDGANDGASGVAV
                     LLEIARLLPASGLETGIDILLVDLEDYGQEEDDTRFPRQENTWCLGSQFWAKNMHRSD
                     YFAQYGILLDMVGGVNPVFPKEGTSLYYAPDVLSKVWNIAQNMGYGQFFINEPAPQTI
                     DDHLYINQLANIRTIDIVHYDNVKKEYPPYHHTHGDNLSIIDKNTLNMVGRVVMQTIF
                     SK"
     gene            1594..>2324
                     /locus_tag="PKU82_12530"
     CDS             1594..>2324
                     /locus_tag="PKU82_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOZ83735.1"
                     /translation="MPVNFSEILVVGVSSRALFNLEKENEIFLKEGISGFRKYQLAHE
                     HEPLQPGTAFHLVQSLLHLNESAKRRIVEVIVMSRNSPETGVRVMNSVGKYKLDITRM
                     AFSGGEPLAPYIDAFDVDLFLSKDLKDVQNVIDSKKSAAAYIYEPPTEFKPTDNRVKI
                     AFDADAVLFSDESEHRYKTEGIEAFHKYEQEHQDVPLAEGPFAKLLIKLSKIQDEMPP
                     PIELSPLRLAIVTARNAPSHIRVIKT"
BASE COUNT          768 a          405 c          484 g          667 t
ORIGIN      
        1 ccaatggcag ttcgcttttt tatgatgcaa acgcattaca gaagtacgct cgatttcagt
       61 aatgaagcac tgcaggcagc agaaaaagga tatcagcgat tgatgtctgc cgttaaaaaa
      121 ctcaacagcg taaaggcctc agacgttacc tctgtaagtt tatcttcaat aagaaaaaat
      181 gcctacgatg ccatgaacga tgacttcaac agtccggtta tggtagcgca gttgtttgag
      241 ttagttcgga taatcaactc cgctaatgat ggaaaagaaa agatgacgaa agaagatatt
      301 gcagaggcaa aatcaattat tcagcatttt gtttttgaca ttgcaggact gatggatgat
      361 acggtttctt caggcgatca gtctgtgctt gataatgtaa tgcaattggt tcttgaaatg
      421 cgcaatcagg caaaagcaaa aaaagaattt tctgtttcgg accatattcg caacagttta
      481 aatgcaatgg gaataaatgt aaaagactcg aaagatggca gcacttggga aaaaatgtaa
      541 ttttttaaat ttaattgctg ttgctgtatt acttgtggtg ggttcgtgta aacagcctgc
      601 acaagaaagc acagagacaa aacaggaaac tcctgctact gtaaatattc ctgtgccggc
      661 atttaatgct gactctgctt tacattattt acagacacaa gttgcatttg gtccgcgtgt
      721 gcccggaacc gatgcacatg cacaatgcgc tgattacctt tttcaaaaac taaagtcatt
      781 tggtttgcag acacagattc agtctgaaac aataactaca ttcgacagta aaaaattcag
      841 actgaaaaat attattgctg aatataaacc agaggcaaaa caaagagttt tgttgctggc
      901 acattgggac acacgcccct gggccgatca tgattcagtg cgaaaggatc aaccttttga
      961 tggtgcaaac gatggtgcaa gtggtgtggc agtattgctt gaaattgcaa gactactgcc
     1021 tgcatcagga cttgaaacag gaatagatat tttattggtt gaccttgaag attacggaca
     1081 ggaagaagac gacacacgtt ttcctcggca agaaaatact tggtgcttag gttcacaatt
     1141 ctgggcaaag aatatgcatc gctcagatta ttttgctcag tacggaatac ttctcgatat
     1201 ggttggtggt gttaatcctg tttttccaaa ggaaggaaca tcattgtatt atgcacctga
     1261 tgtgttgagt aaagtttgga acattgctca gaacatgggt tatggacaat tttttattaa
     1321 tgaacctgcc ccacaaacaa tagatgacca tttatacatc aatcaacttg ccaatatcag
     1381 aaccattgac atagtgcatt acgataatgt aaagaaagaa tatccacctt atcatcatac
     1441 gcatggcgac aatctttcca tcattgataa aaacacatta aacatggttg gaagggtagt
     1501 gatgcaaaca attttcagta agtaaggttt atatatttaa atacttataa agttgtttca
     1561 ttcaaattga attgatagat aaatcaaaag taaatgccgg taaatttttc tgaaatatta
     1621 gtagttggtg tttccagccg agcgcttttt aacctcgaaa aagagaatga aatatttctt
     1681 aaagaaggaa tatcagggtt tagaaaatat cagcttgctc atgaacatga gcctctgcaa
     1741 cctggcactg cttttcatct tgttcagagt ttgcttcacc tgaatgagag tgcaaaaagg
     1801 agaatcgttg aagtgattgt tatgtctaga aacagtccgg aaacaggagt cagggtaatg
     1861 aattctgtag ggaaatataa attggatatt acgcgaatgg ctttcagtgg tggagaacct
     1921 ttagcaccct atattgatgc ctttgatgtt gacttatttc tgagcaaaga tttgaaagat
     1981 gtacaaaatg ttattgattc aaaaaaatca gcagctgcat atatttacga gccaccaaca
     2041 gaatttaagc caacagataa cagagtaaaa attgcttttg atgccgatgc tgttttgttt
     2101 tctgatgaat cagaacacag atataaaaca gaaggaatag aggcatttca taagtacgag
     2161 caagaacatc aggatgtgcc attggcagaa ggtccatttg cgaagttgct catcaaattg
     2221 tctaaaattc aggatgagat gcctcctcca attgagcttt cgccattgcg tttagcaatt
     2281 gtaacagcac gaaatgcacc ctcacacatt cgtgtcatta aaac
//
LOCUS       DAOOOL010000269         2244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6047_length_2244_cov_84.172682, whole genome shotgun sequence.
ACCESSION   DAOOOL010000269 DAOOOL010000000
VERSION     DAOOOL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6047_length_2244_cov_84.172682"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..949
                     /locus_tag="PKU82_12535"
     CDS             101..949
                     /locus_tag="PKU82_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HOZ83736.1"
                     /translation="MLLVADSGSTKTNWLLTDRENFTTEIETIGFNPFFHSTDFILNE
                     LKKNQKLVKYADAIKDIKFFGSGCSSDDRKNIVAAAFKIIFPNATLLVDHDMLGAALA
                     ACFDTAGLVAILGTGSNIAFWDGKHLHPTNHGLGYILGDEGSGSYFGRKLMTHFLYGI
                     MPNDLKEKFFERYHLTKESAIKNVYHLAGANVYMASFAKFLSDEASHPYIQNLIYKGL
                     TEFADTSILAYPQHQNYPVHFIGSIAFHFRNILEQIASEKKFIVGKTIIRPVDDLLVY
                     FTQEEK"
     gene            1035..1973
                     /locus_tag="PKU82_12540"
     CDS             1035..1973
                     /locus_tag="PKU82_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83737.1"
                     /translation="MKKQLFLITSAVVLTTFFYSCKKEDQQALDSKTKQFNDDSNNYK
                     AESDQADNDINNTINEIPAFGRGAGVVSSPLCGVTIDSSQLSQKILFFNFDGITPCFS
                     PSRTRSGQIKVQLTTGTNWHLAGSVLTITYNNFKITRLSDNKSITFNGVKTLTNVNGH
                     NWLTFLAGTSSFKYQSRAFNINVAYDDGSTATWNQANITEWLYVPSGNHGAYLIFTAN
                     GDSTVNNYSNSSMWGTNRYGQAFTTNYTTQIISNTYCGLWRPISGELIHHVNATDFAL
                     TLGVDQSGNASTLDCAYGFKVSWTSNGNSNSVVLSY"
     gene            2050..>2244
                     /locus_tag="PKU82_12545"
     CDS             2050..>2244
                     /locus_tag="PKU82_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A phosphate methyltransferase"
                     /protein_id="HOZ83738.1"
                     /translation="MALQEEMEKQGLWLFRYRSTLPLLLLAGGLSYYLWSKMHHIRPF
                     PENSRSEEWFLYVCMAVSFLG"
BASE COUNT          729 a          392 c          400 g          723 t
ORIGIN      
        1 gaccttgaaa aagtagttaa acaacacaat cctttcgaca agcgattggt gtcatacaat
       61 tatttcttat ctttataaat catcttctaa tataaacttt atgcttttag ttgcagacag
      121 tggctctaca aaaacaaact ggcttttaac agacagagaa aattttacta cagaaattga
      181 aactattgga tttaatccgt tttttcatag tactgatttc attttaaacg agcttaaaaa
      241 aaatcaaaaa ttagttaagt atgccgatgc aataaaagat attaaatttt ttggttcggg
      301 ttgttccagc gatgacagaa aaaacattgt agcagcagca tttaaaatta tttttcccaa
      361 tgcaacactt ttagttgacc acgacatgtt gggtgcagcc ttagctgcat gctttgacac
      421 agcagggtta gttgcaattt taggaacagg atcgaacatt gctttttggg atggtaaaca
      481 ccttcaccct accaaccatg gattgggtta tattttaggt gatgagggta gcggaagtta
      541 ttttggcaga aagctcatga cacatttttt gtatggtatt atgccaaatg atttaaaaga
      601 gaaatttttt gagcgctatc atctcacaaa agaaagtgct attaaaaatg tttaccatct
      661 ggcgggagca aatgtatata tggcatcatt tgcaaaattt ttatctgatg aagcttcgca
      721 tccttacata caaaatctga tatacaaggg tcttactgag tttgccgaca ccagtattct
      781 cgcctatcct caacatcaga attatccggt acattttatt ggcagcatag catttcattt
      841 tcgtaatatt ttagagcaaa ttgcatcgga aaagaaattt attgtaggca aaaccattat
      901 acgacctgtt gatgacttgt tggtttattt cacacaagaa gaaaaataaa caatattgta
      961 tataatgtga cgttttttta aaaaacaaac ttctgatgct taaaatgatt acttttattc
     1021 taaattctgt aaaaatgaaa aagcaactct ttttaattac atcagcagtg gtacttacca
     1081 cattctttta ctcctgcaaa aaggaagacc agcaagcatt agattcaaag acaaaacagt
     1141 ttaatgatga ttctaataat tacaaagcag aatctgatca ggctgacaat gacatcaaca
     1201 acaccattaa cgaaatccct gcatttggca gaggtgctgg tgttgtatca agcccgcttt
     1261 gtggtgtaac aattgattca tctcagcttt cacagaaaat tctgttcttt aattttgatg
     1321 gtatcacacc ttgtttcagt ccgtcacgca cacgatcagg tcaaattaaa gttcagttaa
     1381 caacaggtac aaactggcat ctggctgggt ctgttcttac cattacttac aacaatttta
     1441 aaataacccg actcagcgat aataaatcca tcacctttaa tggtgttaaa acactgacaa
     1501 atgttaacgg acacaactgg ctaacttttc tggccggtac aagcagtttt aaataccaaa
     1561 gccgtgcttt taatattaat gtggcttatg atgatggctc aactgccact tggaatcagg
     1621 caaacattac tgaatggttg tatgttccat caggaaatca tggtgcttat cttattttta
     1681 ctgccaatgg cgattctaca gtaaataact attcaaacag cagcatgtgg ggaacaaaca
     1741 gatatggtca ggcttttaca accaattata ctacacaaat aatttcaaac acctattgtg
     1801 gtttgtggcg gcctatttca ggtgagttga ttcatcatgt aaatgctaca gattttgcac
     1861 tcacgttggg agttgatcaa agtggaaatg caagcacact cgactgtgct tatggattta
     1921 aagtatcatg gacaagtaat ggaaattcta attcagtagt ccttagctat taacactatt
     1981 ttgttttaaa atgaaaacag aatcttatgg taaggttctg tttttatttt tgtgccatat
     2041 tcaaacagta tggccttaca agaagagatg gaaaaacaag ggctatggtt attcaggtac
     2101 cgtagcaccc tacccttact acttttagca ggaggcttgt cttattattt atggagtaag
     2161 atgcatcaca ttagaccatt tccggaaaat tcaaggtctg aagaatggtt tttatacgtt
     2221 tgtatggctg tatcgttttt aggc
//
LOCUS       DAOOOL010000270         2229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6105_length_2229_cov_66.765409, whole genome shotgun sequence.
ACCESSION   DAOOOL010000270 DAOOOL010000000
VERSION     DAOOOL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6105_length_2229_cov_66.765409"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKU82_12550"
     CDS             <1..221
                     /locus_tag="PKU82_12550"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647666.1"
                     /note="catalyzes the oxidative ring opening of
                     3-hydroxyanthranilate to 2-amino-3-carboxymuconate
                     semialdehyde in the final enzymatic step of quinolinate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HOZ83739.1"
                     /translation="IGLVVERYRKPDEMDACMWFCEKCNHKLYEEFFPLKDIVNQLPV
                     VMNDFYNSLEKRTCKQCSTVMEPPVPAK"
     gene            221..646
                     /gene="ybeY"
                     /locus_tag="PKU82_12555"
     CDS             221..646
                     /gene="ybeY"
                     /locus_tag="PKU82_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOZ83740.1"
                     /translation="MQTEVNFFKEDVQFRLNDKDAISSWVVGCIKKHRKKPGTLNFIF
                     CSDELLYSMNIKYLNHKTLTDIITFDYTEDATISGDIFISIDRVRENAKLLKNSIKDE
                     LHRVMIHGVLHLCGYSDKKPADKQKMTKAENKYLSLRTF"
     gene            733..>2229
                     /gene="mnmG"
                     /locus_tag="PKU82_12560"
     CDS             733..>2229
                     /gene="mnmG"
                     /locus_tag="PKU82_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOZ83741.1"
                     /translation="MIQEYDIVVVGAGHAGCEAAAAAANMGSRVLLITMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVSDKSAIQFRILNRSKGPAMWSPRTQNDRMLF
                     ASEWRQMLENTPNVDFWQDMVSALIIEDNKLKGVVTSMGHQVRCKAVVLTNGTFLNGI
                     IHIGEKRFGGGRSGEKAATGITEQLVSLGFVSGRMKTGTPPRLDGRSIDYSKLEEQKG
                     DENPGKFSFTDTIPLTTQRSCHITYTNDEVHNILKTGFEKSPMFTGRIQGLGPRYCPS
                     IEDKINRFADKDRHQLFIEPEGWNTVEIYLNGFSSSLPEEVQYAALKKIPGLENVKMF
                     RPGYAIEYDYFPPLQLNLTLETKKIEHLYFAGQINGTTGYEEAACQGLIAGINAHLKI
                     NNQNPLILKRSDAYIGVLIDDLVTKGTDEPYRMFTSRAEFRTLLRQDNADIRLTPVSH
                     QIGLADAQRFSRVTEKKQAVDRIIEACRTESISPSEINAYLNSKESAEL"
BASE COUNT          763 a          408 c          453 g          605 t
ORIGIN      
        1 ctattggttt ggtagtagag cgctatcgta agcccgatga aatggatgcc tgcatgtggt
       61 tttgcgaaaa atgcaatcac aaactatatg aagagttttt cccattgaaa gatattgtaa
      121 atcaattacc tgtggtgatg aacgattttt acaattcgct cgaaaaaagg acttgcaagc
      181 aatgcagtac ggttatggag cctcctgtac cggctaagtg atgcaaactg aagtgaattt
      241 ttttaaggaa gatgtacaat ttcgcttgaa tgacaaggat gcaatatctt cttgggtagt
      301 gggctgtatt aaaaagcacc gtaaaaaacc gggtacctta aattttattt tttgctctga
      361 tgagttattg tactcaatga atatcaaata tctgaatcac aaaacattaa cagatattat
      421 cacctttgat tacacagaag atgctactat ttcaggtgat atttttatca gcatagacag
      481 agttagagaa aacgctaaat tattgaaaaa cagcataaaa gatgagctac accgtgtcat
      541 gatacacggt gtacttcatt tatgtggata tagtgataaa aagcctgccg ataaacaaaa
      601 aatgaccaag gcagaaaaca aatacctatc tttgcgaacc ttttgattta aagggattta
      661 cgaatttaat tcaactatat tttaaatgtt tcacgtgaaa ccaatcgttt tggtttttaa
      721 ttgtgctatt atatgattca agagtatgat atcgtagtgg ttggggccgg tcatgcaggt
      781 tgcgaagctg ctgctgctgc tgccaatatg ggtagtcgag ttttattgat taccatgaac
      841 atgcaaacca ttgcacaaat gagttgcaat cctgctatgg gcggtgtagc aaaggggcaa
      901 attgttagag aaatagatgc tttaggtggc tattcaggta tagtctctga taaatcagcc
      961 atacaattca gaatacttaa ccgctctaag ggacctgcta tgtggagtcc ccgtacacag
     1021 aatgacagga tgctatttgc ctcagaatgg cgacagatgc ttgaaaacac acctaatgtt
     1081 gacttctggc aggatatggt cagtgcttta attattgaag ataacaagct aaaaggtgtt
     1141 gtaactagca tggggcatca ggtacgatgc aaagcagtgg tattaaccaa tggaaccttt
     1201 ctaaatggca ttattcatat aggtgaaaaa cgctttggcg gtggccgttc aggagaaaaa
     1261 gctgcaacag gcatcacaga acagttagtt agcctaggtt ttgtcagtgg aagaatgaaa
     1321 acaggtacgc ctccacgctt agatggtcgc agtatagatt actcaaaatt agaagaacaa
     1381 aaaggagacg aaaacccagg taaattctcc tttacagaca ccatacccct cacaacacaa
     1441 cgtagttgtc atataaccta caccaacgat gaagtacata atatcttaaa aaccggattc
     1501 gaaaaatcgc caatgtttac aggcagaatt caaggacttg gaccaagata ctgcccttca
     1561 atagaagata aaatcaatcg ctttgcagac aaagaccgcc atcagctatt tatagaaccc
     1621 gaaggctgga atacagtaga aatttatctc aatggatttt catccagtct gcccgaagaa
     1681 gtgcaatatg ccgcactaaa aaaaataccc ggacttgaaa acgtaaaaat gttcagaccc
     1741 ggatatgcca tagaatatga ttacttccct cctcttcaat taaacctgac cttagaaaca
     1801 aaaaaaatag aacatcttta ttttgcagga caaataaacg gaaccacagg ctatgaagaa
     1861 gccgcctgcc aaggtttaat agcaggaata aatgctcacc ttaaaattaa taatcaaaac
     1921 ccattaatcc taaaacgctc tgacgcttac attggggtac tgattgatga tttggtaaca
     1981 aaaggaactg atgaacctta tcgtatgttt acaagccgtg cagaattccg gacgctttta
     2041 aggcaagata atgcagatat caggctgact cctgtaagtc atcagattgg tttagcagat
     2101 gctcaaagat tttcacgtgt aacagaaaaa aaacaggctg tggacagaat aattgaagct
     2161 tgcagaacag aaagtatttc gccttcagaa attaatgcct accttaattc aaaggagtct
     2221 gcagaactt
//
LOCUS       DAOOOL010000271         2220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6131_length_2220_cov_83.248961, whole genome shotgun sequence.
ACCESSION   DAOOOL010000271 DAOOOL010000000
VERSION     DAOOOL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_2220_cov_83.248961"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..571)
                     /locus_tag="PKU82_12565"
     CDS             complement(164..571)
                     /locus_tag="PKU82_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83742.1"
                     /translation="MRKNKQVLFLLTLVMLAFTACTPDDDSTGDDRDKFTGSWTCRDS
                     NTTTGVTDPLYSVNISKVGIDDTIMITNFYNLGGSTFVKAVVSGTSVVIPNQYDDSYL
                     ISGSGIYSNSEVKLNYSASLGSSMNNISAKYSK"
     gene            complement(568..2217)
                     /gene="hutU"
                     /locus_tag="PKU82_12570"
     CDS             complement(568..2217)
                     /gene="hutU"
                     /locus_tag="PKU82_12570"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOZ83743.1"
                     /translation="MSKLKTPTGNTISCKGWVQEAALRMLHNNLDPDVAERPEDLIVY
                     GGTGKAARNVESFHLIVKALRDLNEDETLLVQSGKPVGILPTHKDAPRVIIANSNLVG
                     HWANWDYFRELEAKGLIMYGQMTAGSWIYIGSQGIVQGTYETFGEVARQHFGGSLKNT
                     LNVTAGLGGMGGAQPLAITMNEGTCLAAEMEEWRVDKRIQTRYLDEKYSDLDKAIDRA
                     VEATKKGEAISIAYIGNAIDMLARLIERNIIPDTLTDQTSAHDALVGYFPEGLNVTDA
                     KVLRDSNPEEYIKRSKQSMRKHVELMIALQKKGAVTFDYGNNIRQQALEMGLKNAFDF
                     PGFVPAYIRPLFCEGKGPFRWAALSGDPNDIYETDKKILELFPENQGLHRWITMAQKR
                     IAFQGLPARICWLGQGEREKAGLAFNELVKSGKVKAPIVIGRDHLDCGSVASPNRETE
                     AMKDGSDAIADWPILNALINTAGGASWVSLHHGGGVGIGYSIHAGMVIVADGTADAAT
                     RLKRVLHNDPAMGVLRHLDAGYDIAADTARKHRLNLRDRLA"
BASE COUNT          637 a          516 c          380 g          687 t
ORIGIN      
        1 gacatcagta aacttaaaac acctacagga aatactatta gttgtaaagg ttggggatgg
       61 cagcataaac catacaacta ttatgcaaca attttaagaa tgtatttccc tgcagattat
      121 gcaagactga aaagaaaata tttatctgaa aatctctgat aaattatttc gaatactttg
      181 ctgaaatatt attcatacta ctgcctaagg atgccgaata attgagttta acttcgctgt
      241 tgctgtaaat tcctgaacct gaaatcaaat aactgtcatc atactgattc ggtataacaa
      301 cactagttcc acttacaaca gcttttacaa aagttgatcc accaagattg tagaaatttg
      361 taatcattat cgtatcatca ataccaacct ttgaaatatt gacagaataa agtggatccg
      421 ttacaccggt tgttgtattt gaatcacggc atgtccagct tccggtaaat ttatctctgt
      481 catcgcctgt gctatcatca tcgggtgtac aggctgtgaa tgccaacatc accaacgtta
      541 ataaaaataa tacttgctta ttttttctca tgccaatcta tcacgcaaat ttaaacgatg
      601 ttttcttgct gtatctgcag caatgtcata acctgcatca agatggcgca gaactcccat
      661 agcagggtca ttgtgcaaca cacgttttaa tcttgttgct gcatcagcag taccatcagc
      721 gacaattacc atacctgcat gtatgctgta gccgatacct actccaccac catgatgtaa
      781 actaacccaa cttgcaccac cggcagtgtt gatcaatgca tttagtattg gccagtctgc
      841 aatggcatcg ctaccatctt tcattgcttc tgtttcgcgg tttggcgatg caacacttcc
      901 acaatccaaa tggtcgcgac caataactat aggtgccttt acttttcctg atttaaccaa
      961 ttcgttaaaa gcaagacctg ctttttcgcg ttcaccttgt ccaagccaac agatgcgtgc
     1021 cggcaatccc tgaaatgcaa tgcgcttttg tgccatagtt atccaacggt gtaagccttg
     1081 gttttcagga aacaattcca gaattttttt atcggtctca taaatatcat tagggtcgcc
     1141 actgagtgca gcccaacgaa acggaccttt gccttcgcaa aataacggac gtatgtatgc
     1201 aggaacaaat cccggaaaat caaaagcatt ttttaatccc atctccaatg cctgctggcg
     1261 aatgttgttt ccataatcaa acgtaacagc accttttttc tgcaaggcaa tcatcaattc
     1321 tacatgcttg cgcatgcttt gtttagaacg ctttatgtat tcttcaggat tagaatcacg
     1381 taaaactttt gcatctgtca catttaatcc ttcagggaaa tatcctacca aagcatcatg
     1441 tgcagatgtc tgatctgtta aggtatcggg aataatattt ctttcaatca atcgtgccag
     1501 catatcaata gcattgccaa tataagcaat tgaaattgct tcaccctttt ttgttgcttc
     1561 aacggcacgg tcaatggctt tatccaaatc agaatatttt tcgtcaagat aacgcgtttg
     1621 tattcttttg tcaacacgcc actcttccat ttctgctgca agacatgtgc cttcattcat
     1681 tgttattgcc aatggctgtg caccacccat tccaccaaga cctgctgtta cattcaaggt
     1741 gttttttagc gacccaccaa aatgctgacg tgccacttca ccaaatgttt cataagtacc
     1801 ctgaacaatt ccttgcgagc cgatgtaaat ccaactgcct gctgtcatct gtccgtacat
     1861 tatcaaccct tttgcttcca actcgcggaa ataatcccag ttagcccaat ggccaacaag
     1921 gttggagttg gcaataatta ctcttggagc atctttatgc gttggtaaaa ttccaaccgg
     1981 tttacctgat tgcactaaca atgtttcgtc ttcatttaaa tcacgcaggg cttttacaat
     2041 gagatgaaat gactctacat ttcgtgcagc ttttccggtg ccgccataaa caattaaatc
     2101 ttcaggacgt tctgccacat caggatcaag gttgttgtgc aacatgcgca atgcagcttc
     2161 ctgaacccaa cctttacaac taatagtatt tcctgtaggt gttttaagtt tactgatgtc
//
LOCUS       DAOOOL010000272         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6204_length_2204_cov_35.543043, whole genome shotgun sequence.
ACCESSION   DAOOOL010000272 DAOOOL010000000
VERSION     DAOOOL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6204_length_2204_cov_35.543043"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PKU82_12575"
     CDS             <1..910
                     /locus_tag="PKU82_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOZ83744.1"
                     /translation="LNNDANNLLIIQDKSLAIGYTMEFEEMWGSSTLQPNPANSKFGP
                     DKKDNTPHEYNIGGNRVESYFSPSDNVNNQIMTTVETADQQMQFALLVFTRFDVAYAA
                     EDRIVNHGVDAYGIVDDTGSGGGQAYSILNAVMGSKLMLYNHATQTGLLHHKYLIVDQ
                     NNPSSDPLVLTGSHNWSTTANQKNDENTLIIHNRNIANQYYQEFVKRFTDNGGVLGLS
                     ETEEGMTTTLYPNPASDHLFISLNHALENNYQFGLFDVAGKQVMQMNLQQGNSGIISL
                     GRLNKGIYFYQIRGEKQINSGKLIIN"
     gene            1080..1778
                     /locus_tag="PKU82_12580"
     CDS             1080..1778
                     /locus_tag="PKU82_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOZ83745.1"
                     /translation="MSILLQITQAVTDSATALQPAVKAEQSISFLDLVIKGGPIMIPL
                     GLLSVAAVYIFFERYFTIRKSSNIDKNFINQIKDFVHTGNVDAAKALCRNTNHPVARM
                     IEKGLLRLGKPIRDIESSIENVGKLEIYKLEKNLSFLGTIAGIAPMFGFLGTIFGVIK
                     IFYQISLENSLQIDTISGGLYVKMITSAAGLLIGVAAYAGYHYLISLVDRAVNKMEIN
                     AVEFIDLLEEPVKK"
     gene            1809..>2204
                     /locus_tag="PKU82_12585"
     CDS             1809..>2204
                     /locus_tag="PKU82_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOZ83746.1"
                     /translation="MALNLRRRNKMTGEVFVGSLNDIMFFLLLFFLITSTMATPNVLK
                     LLLPNAKTSKPAVKHPVTISVTADMQYAVNNQVVAYDQLESSILTQLQGQEDPTAILK
                     VDKSVQVQGLVDLLDIGARNKIKMVLATNT"
BASE COUNT          704 a          370 c          445 g          685 t
ORIGIN      
        1 actcaataac gatgcaaaca atttgttgat tattcaagat aaatcgcttg ccattggtta
       61 cacaatggag tttgaagaaa tgtggggcag cagtacattg cagccaaatc ctgccaattc
      121 aaaatttggt cctgataaaa aagataacac tccacatgaa tacaatattg gaggtaatcg
      181 tgtcgaatct tatttcagcc cttctgataa tgtaaacaat caaataatga caacggttga
      241 aacagcagat cagcaaatgc aatttgcttt attggtgttt acacgttttg acgtggccta
      301 tgctgcagaa gaccgtattg tgaatcatgg tgtagatgct tatggaattg ttgatgatac
      361 aggtagtgga ggtggtcagg cctatagtat tttaaatgct gttatgggtt caaaactgat
      421 gctttacaat catgcaacac aaacaggatt gttgcaccat aaatacctga ttgttgatca
      481 gaacaatcct tcaagtgacc cattagtgct gacaggctcg cataactgga gtacaactgc
      541 caatcagaaa aatgatgaaa atactttaat catacacaac agaaacattg ccaatcagta
      601 ctatcaggaa tttgttaaac gatttacaga taatggcggt gtgcttggtt tgagtgaaac
      661 agaagagggt atgacaacga ccttatatcc caatcctgca tccgatcatt tgtttatcag
      721 ccttaatcat gcacttgaaa ataattatca attcgggctt tttgatgttg ccggtaagca
      781 ggttatgcaa atgaatctgc aacagggcaa ttccggaatt atttctttag ggagattgaa
      841 taaaggcatc tatttctatc aaatcagagg tgaaaaacag ataaattcag gtaaactgat
      901 tatcaattaa acgaacaata atttttcagg aagttcaatt ttaggttaaa taatttatac
      961 ttttttcctt ttccttccgt tagccttatc aacaaaactg tgtttaaaat agcagtgaca
     1021 cttgattatt taataagggc atgggcgtac ttttactgtt ctaaaatttc gaattcaaca
     1081 tgagcatttt attgcaaatt actcaagctg ttactgatag cgccactgca ttacagcctg
     1141 cggtgaaggc cgagcaatca atttcgtttc ttgatttagt tataaagggc ggaccaataa
     1201 tgattccatt aggattgttg tctgttgctg ctgtttatat ttttttcgag agatatttta
     1261 ctatcagaaa atcatccaac attgacaaaa attttatcaa tcagataaaa gactttgtcc
     1321 atacaggtaa tgttgatgca gcaaaggcat tgtgccgaaa cacaaaccat cctgtggcgc
     1381 gtatgattga aaaggggttg ttgcgtctgg gtaaacctat ccgtgatata gaatcatcaa
     1441 tagagaatgt tggtaagctc gaaatttata aacttgaaaa gaacctgagt ttcttgggga
     1501 cgatagcagg tattgcaccc atgtttggtt tccttggaac aatcttcgga gtaataaaaa
     1561 ttttctatca gatatcgttg gagaatagtt tgcagataga caccatctca ggtggtttgt
     1621 atgtgaaaat gataaccagt gcagccggtt tattaattgg tgttgcagct tatgcaggtt
     1681 atcattatct gatttcgtta gttgatcgcg cagtaaacaa aatggagatt aacgctgttg
     1741 agtttattga cctactcgaa gagcctgtaa aaaaataatt gtgaaattca gtttataaaa
     1801 gcatcattat ggcattgaat ctgagaaggc gaaataaaat gacaggggaa gtttttgttg
     1861 gctccctcaa tgatattatg tttttccttt tattattttt tctgattaca tcaacaatgg
     1921 caacaccaaa cgttttgaaa ctgttgttgc cgaatgcaaa aaccagcaag cctgctgtga
     1981 agcatccggt gacaatttct gttactgccg atatgcagta tgcagtaaac aatcaggtgg
     2041 tggcttatga tcagttagaa agttctattc ttacacaatt gcaaggtcag gaagatccaa
     2101 ctgcaatttt gaaagttgat aaaagcgttc aggtgcaagg cttggttgac ttgcttgaca
     2161 ttggtgcccg caataaaata aaaatggtgc ttgctaccaa caca
//
LOCUS       DAOOOL010000273         2188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6258_length_2188_cov_27.646976, whole genome shotgun sequence.
ACCESSION   DAOOOL010000273 DAOOOL010000000
VERSION     DAOOOL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6258_length_2188_cov_27.646976"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKU82_12590"
     CDS             complement(<1..654)
                     /locus_tag="PKU82_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOZ83747.1"
                     /translation="MRKVIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSIVGFIK
                     DGERKVGDPAKRQAITNPKNTVYSIKRFMGETYDHASKEKDRVPYEVVKGDNNTPRIK
                     INDRQYTPQEISAIILQKMKKTAEDYLGNEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVLRIINEPTAAALAYGLDKKNKDQKIVVFDCGGGTHDVSVLELGDGVFEVKSTDGD
                     "
     gene            complement(786..1775)
                     /locus_tag="PKU82_12595"
     CDS             complement(786..1775)
                     /locus_tag="PKU82_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83748.1"
                     /translation="MNKLLISLCLLCLSLSSCKTDVDLLAPYKEVMVIYGVLDPTDSV
                     QYIRINKAFLGEGNAYTFAQVADSFQYADVLDVKLERYKNNNFAGAINLERFEGPPLK
                     DGIFATAPNYLYRTTGLDSIYQDSEYKLVVTNTQTGNVATSKTVIVSKVGSITNPASF
                     QTAVDFADPDNQFIAKFTRGNNAKYYDLNMRFNYYETEIANPSNTQIKSVFFRVNGVE
                     YLSNNSLEIAVPYSAFYSSLKRTIPVDPLVTRTARSVDLIWVGGAEELYTYYQVNRPP
                     TGINQSIPQYTNIDGGIGIFSSKYSDAMNNKLLSSRSLDSLSNGAITKDLGFQ"
     gene            1872..>2188
                     /locus_tag="PKU82_12600"
     CDS             1872..>2188
                     /locus_tag="PKU82_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83749.1"
                     /translation="MRKVFFILIIFIATLSDFSLVQAQTVNEKFDRLDLSENFDTSSG
                     MWTFMSNSENLFLVQEGEYILNRKAPEKPYAIIANYDYNQLAFKMVTSLKIEKANDQN
                     GSVG"
BASE COUNT          639 a          424 c          431 g          694 t
ORIGIN      
        1 atcaccatca gtagatttta cttcaaacac gccatcaccc aattctagca cagaaacgtc
       61 atgtgtacca ccaccacagt cgaacactac aattttctgg tctttatttt tcttgtcaag
      121 accgtaagct aatgctgctg cagtaggctc gttgatgatg cgcaacactt ttagtccggc
      181 aatctctccg gcttcttttg ttgcctgacg ctgcgagtcg ttgaagtagg caggcacagt
      241 gataactgct tctgtaactt cattgcccaa ataatcttct gccgtttttt tcattttctg
      301 aagtatgatg gcagagattt cctgtggcgt atattggcga tcgttaattt taattcgtgg
      361 tgtgttgtta tcgcctttta caacttcgta aggtacacgg tctttttcct tgcttgcatg
      421 atcgtatgtt tcgcccatga agcgctttat agaataaacc gtatttttgg gattggtgat
      481 agcctgacgt ttagcaggat cgccaacttt acgttcgcca tctttaataa atccaacaat
      541 ggaaggggtt gttctgcgac cttcgctgtt tggaataact acaggctcgt tgccttccat
      601 tactgcaacg catgagttgg ttgttccaag gtcaattcca ataacttttc tcattttctt
      661 aaatactttt tgaggttttt tatcctagtg tcaagcctta tgccaatagg atatttatga
      721 cattttactg ccattacagt caaatcacta aggcaactaa ttaaaaatgt gacaaaaaga
      781 cacggttatt gaaagcctaa atctttggtt atggcaccat tgctaagcga atctaacgaa
      841 cgggaagaaa gcaatttgtt attcatcgca tcagaatact tactgctgaa aattccaatg
      901 cctccatcaa tattggtgta ttgcggaata gattggttga tgcctgtagg tgggcggtta
      961 acctgataat aagtatataa ttcttctgca ccaccaaccc aaattaaatc aacagagcga
     1021 gctgtgcgtg ttactaatgg gtcaacagga atagttcttt ttagcgatga ataaaatgca
     1081 gagtagggaa ctgcaatttc gagactgttg tttgagagat attcaacacc attaactctg
     1141 aaaaatactg atttaatttg agtgtttgaa gggtttgcaa tttctgtttc gtaatagttg
     1201 aatcgcatat tcaaatcata atacttggca ttgttgcctc ttgtaaactt tgcaataaat
     1261 tggttgtcgg ggtctgcaaa atcaacggct gtctgaaacg atgcgggatt agtgattgaa
     1321 cccaccttac tcacaataac agtttttgaa gtagccacat tgcctgtttg tgtattggta
     1381 acaacaagtt tgtattcact gtcctgataa atagagtcga gacctgtagt gcgataaaga
     1441 taatttggtg ctgtagcaaa aatgccgtcc ttaagtggag gtccttcaaa tcgctctaag
     1501 ttaatggcac ctgcaaagtt gttgttttta tagcgttcta atttaacatc caacacatcg
     1561 gcatattgaa aagagtctgc cacctgtgca aatgtatagg cattgccttc gccaagaaaa
     1621 gctttgttga ttctgatata ttgaacactg tctgtagggt caagcacacc gtaaataacc
     1681 atcacttcct tatagggagc cagtaaatcc acatcagttt tgcacgatga aagcgaaaga
     1741 cagagtaaac aaagagatat taataactta ttcatggctc aaaagtagaa gatgactgcc
     1801 gataaaaaaa atatgtaatt ttcacctcgc aaattttaac atatccttat catataaacc
     1861 actgttgttt gatgcgtaaa gtatttttta ttctgataat ttttattgcc acactatctg
     1921 atttcagcct tgtgcaggcg cagaccgtaa atgaaaaatt cgatcgcctt gacctgagcg
     1981 aaaacttcga caccagcagt ggtatgtgga cgtttatgtc aaactcagaa aatctttttt
     2041 tagtacagga aggtgagtat atcttaaatc gcaaagcacc tgaaaaacct tatgctatta
     2101 ttgcgaacta cgactacaat cagctggcat ttaagatggt tacctcattg aagattgaaa
     2161 aggcaaacga tcagaatggc tctgtggg
//
LOCUS       DAOOOL010000274         2179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6301_length_2179_cov_28.495292, whole genome shotgun sequence.
ACCESSION   DAOOOL010000274 DAOOOL010000000
VERSION     DAOOOL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_2179_cov_28.495292"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKU82_12605"
     CDS             complement(<1..309)
                     /locus_tag="PKU82_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HOZ83750.1"
                     /translation="MADIKKDILLRVYFVYALIFLFAMAIMGRVFQLQYVEGDYWKQR
                     SDSLTLAYKTIEPSRGNIFSADGSLLATSVPLYDLRMDMKAESITDKIFNAGIDSRLL
                     M"
     gene            complement(316..705)
                     /locus_tag="PKU82_12610"
     CDS             complement(316..705)
                     /locus_tag="PKU82_12610"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOZ83751.1"
                     /translation="MNTIRNTEEKKEKEKPNGSKIKTARKFLSMLNVIGLIDKNMLLR
                     MLPFAFFLMGLALVYIANSYVAEKTIREIDRTSRDIKELRSEYISVKSELMFKSRQSQ
                     VAKEVLPMGIKQLTVPPKKIVLKTAKD"
     gene            complement(707..1621)
                     /gene="rsmH"
                     /locus_tag="PKU82_12615"
     CDS             complement(707..1621)
                     /gene="rsmH"
                     /locus_tag="PKU82_12615"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOZ83752.1"
                     /translation="MDSKAYHIPVMLPECIEGLKIKADGVYVDVTYGGGGHSKAIASL
                     LTTGKVIAFDRDADAIKNSLTTNKNLIVIHSSFSNITTELNSIGISSVDGVLADLGVS
                     SHQFDEPQRGFAFRFDSTLDMRMDVRQQITAATILKTYTAKELQNMFSLYGEVRNAAT
                     VAREIVAYRQHSAIATTADLRKCVSHIVPKSDENGFYARLFQALRIEVNQELKELELL
                     LQQLPDLIKAEGRLCVMSYHSLEDRLVKNFIASGNVQGAVEQDVFGNIISKTFTAINK
                     KPIEASEEEKTNNPRSRSARLRVAERIK"
     gene            complement(1611..2078)
                     /gene="mraZ"
                     /locus_tag="PKU82_12620"
     CDS             complement(1611..2078)
                     /gene="mraZ"
                     /locus_tag="PKU82_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOZ83753.1"
                     /translation="MINLFGVYESTVDAKGRVMLPSPYRKTLEKVLEEGFIIKQNVFR
                     KSLEFFPLQTWNEISQHVGKLNTFLKANQELITLFYYGVRSVELDNNGRFLIVKDHLT
                     YAGIKKDIVLSGANDRIEIWDKRAYHKYIKDNSDNLERLMEERLGGKLPENGQ"
BASE COUNT          637 a          488 c          335 g          719 t
ORIGIN      
        1 catcaataag cgtgagtcaa tacctgcatt aaatatttta tctgtaatac tttctgcctt
       61 catatccata cgtaaatcat acaaaggcac tgatgtggct aacaaacttc catcggcact
      121 aaaaatattt cctcttgatg gctcaatggt tttataggct aaggttaaac tgtcggagcg
      181 ttgtttccaa taatcaccct caacatactg caactgaaaa acacgaccca taatagccat
      241 ggcaaaaaga aaaatcaagg cataaacaaa atatactctc agtaaaatat ctttcttaat
      301 atctgccatg cgctcttagt cttttgctgt tttaagtact attttctttg gcggaactgt
      361 cagttgctta atgcccatag gaagcacttc ttttgctact tgcgactgac ggcttttaaa
      421 catcaactca gactttactg aaatatattc agatcgcagc tctttaatat cgcgtgatgt
      481 gcggtcaatt tcgcgaatgg tcttctctgc aacatagctg tttgcaatat aaaccaatgc
      541 cagtcccatt aagaaaaaag caaatggaag catacgcagc aacatatttt tatcaatcag
      601 cccaataaca ttaagcatag aaaggaactt acgtgcagtc tttattttac tgccgttcgg
      661 cttttctttt tccttttttt cctctgtatt tcttattgtg ttcatgttac tttattcttt
      721 cagctactct taatcgtgca cttcttgagc gcgggttatt ggttttttcc tcttcactag
      781 cctctatcgg ttttttattt attgcagtaa atgtttttga aatgatgttg ccaaacacat
      841 cttgctctac tgcaccttgc acatttcctg aagcgataaa attttttacc agtctgtctt
      901 caagagaatg atagctcatc acacaaagac gaccttctgc ttttattaaa tccggcaatt
      961 gttgcagcaa caattccaac tctttcaatt cctgattaac ttcaattcgt aatgcctgaa
     1021 acaatcttgc ataaaaacca ttctcatcac tcttaggcac tatgtgtgaa acacattttc
     1081 taagatcggc tgtggttgca atagctgaat gctgacgata agcaactatc tccctagcaa
     1141 cggtagctgc attacgcact tcaccataaa gagaaaacat gttctgcaat tcttttgcag
     1201 tatatgtttt aagaattgtt gcagcagtaa tttgctgacg tacatccatg cgcatatcaa
     1261 gtgtactatc gaagcgaaaa gcaaaaccac gctgcggttc atcaaactga tgtgaagaca
     1321 caccaaggtc ggcaagcacg ccatctacag aagatatgcc tatgctgttc agctcggtgg
     1381 ttatatttga aaaactgctg tgaatcacaa tcagattttt attggtggtt aaactatttt
     1441 ttattgcatc ggcatctctg tcgaatgcaa tcacttttcc tgttgtcaat aaagaggcta
     1501 ttgcctttga atgtcccccg cccccataag taacatcaac ataaactcca tctgctttaa
     1561 tcttcaaccc ttcaatacac tccggtaaca tgacaggaat gtgatatgcc ttactgtcca
     1621 ttttcaggca atttgccgcc aagtcgctct tccatcaacc gctctaaatt gtcgctgtta
     1681 tctttaatat atttatgata agcacgctta tcccaaattt caattctatc gtttgcacca
     1741 ctcagcacaa tatctttttt aattcctgca taagtcagat gatctttcac aattaaaaat
     1801 cgtccgttat tatctaactc cacgctgcgc acaccgtaat aaaaaagtgt aatcaactcc
     1861 tgattggctt ttaagaatgt attcagctta cctacatgtt gcgatatttc attccatgtc
     1921 tgaagcggaa aaaactctaa tgattttctg aaaacattct gcttaatgat aaatccttct
     1981 tccaaaactt tctctaatgt ttttctgtaa ggagaaggca gcatgacacg acctttcgca
     2041 tcaacggtgc tttcgtaaac tccaaacaga tttatcatta ttaaatggtg cgttaaaaaa
     2101 ttcttgtgcg aatgtaaaaa taaatttccc acctaccccc actttttacc aaatttgttg
     2161 aaaactttcc cctttctgc
//
LOCUS       DAOOOL010000275         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6515_length_2133_cov_122.152069, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000275 DAOOOL010000000
VERSION     DAOOOL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6515_length_2133_cov_122.152069"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKU82_12625"
     CDS             <1..346
                     /locus_tag="PKU82_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ83754.1"
                     /translation="INNAFDEVYECDHLGLKGGESYQPTISIKTAVSSPHTNKAGVKY
                     VTIAVGDNGRGVPDYVRQRIFEPFFTTKPTGKGTGLGLSLSYEIIESHGGKIDIDSKE
                     GEGTNFIISLPC"
     gene            538..1752
                     /locus_tag="PKU82_12630"
     CDS             538..1752
                     /locus_tag="PKU82_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOZ83755.1"
                     /translation="MKNIKIGILREEKIPHDKRVPFSPEQCKVIKTEYPEVDVVIQPC
                     NYRAFRDDEYRREGIVLQEDLSDCDILIGIKEVPKDKLIAGKKYLYFSHTIKKQPHNR
                     EMLKKIIRDKITLVDYECLVDDHENRIIGFGRYAGIVGAYNGIMAYGLKYGLFNLKAA
                     HQSFGIEELHKELDSIKLPNIKIVITGGGRVANGAIETMGMLNIRKVTPFEFLNYSFR
                     EPVYVQLHSENYYERKDGSDFLKADFYHYPEKYNCTFCDPGSYSSFTDLLVHCAYWNP
                     NAAVLFTKEDMRSPAFRISVIADVTCDIDGSIPSTHRATTIDDKFYGYNPMNEKEEEA
                     FSTDTITVMSIDNLPCELPRDASEGFGKHLIERVIPLLIGEDKGNVIYRATIARNGEL
                     TPRFAYLTDYIS"
     gene            complement(1756..>2133)
                     /locus_tag="PKU82_12635"
     CDS             complement(1756..>2133)
                     /locus_tag="PKU82_12635"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009746054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOZ83756.1"
                     /translation="EASVHLHRTIQQIKKEGMKAGIAINPHTPVASLSDIIFEADLIC
                     MMSVNPGFGGQKFIENTYRKIQELKELIAIHNSKTLIEIDGGVDLNNYKKLIAAGADV
                     LVAGNTVFSSSDPKATIEVLKKG"
BASE COUNT          661 a          348 c          433 g          691 t
ORIGIN      
        1 gattaataat gcttttgatg aagtttatga atgtgatcat cttggattga aaggtggaga
       61 gtcttatcag ccaactattt caataaaaac agcagtatca agtccacata caaataaagc
      121 aggggtaaag tatgttacaa ttgctgttgg agacaatggc agaggtgttc ctgattatgt
      181 aagacaaaga atatttgagc cattttttac taccaaacct actggtaaag gcacgggttt
      241 aggattgagt ttaagctatg aaattattga atctcatggt ggtaaaatag acatagattc
      301 taaagaaggc gagggaacca attttataat ttcacttcct tgttaagaat ttctaaaaat
      361 tattttatta taaaatctta cttgtttttt agacgcaatg ctttcaaatc atcagtaaac
      421 tgaaagcgta ttttactcct tatgcgaaag ggttacttga ttgattttcg aatacatgat
      481 aaatgttgct tttcgtttta atttaaagcg gtatttttga gcagattttt attgaggatg
      541 aaaaatatta aaataggaat actaagagaa gagaaaattc cacacgataa acgtgtacca
      601 tttagccccg aacaatgcaa ggtaattaag acagaatatc ccgaagtaga tgttgtgatt
      661 caaccatgca actatcgcgc tttcagggat gatgaatata ggagagaggg aattgttttg
      721 caggaagatt tatcagattg cgatattctt attggcataa aagaagtgcc taaggataag
      781 ctcattgcag gaaagaaata tctttacttt tcacatacca taaaaaagca gcctcataac
      841 cgtgaaatgc tgaagaaaat aattcgtgat aaaattacac tcgttgacta tgagtgtttg
      901 gttgatgatc atgaaaaccg cattattggt tttggacgct atgccggtat agtaggagca
      961 tataatggta tcatggctta tggtttaaag tatggattgt ttaacttgaa agctgcgcat
     1021 cagagttttg gtattgaaga gttgcataag gaattagaca gcattaaact tccgaatatt
     1081 aaaatagtga ttacaggagg tggtcgtgtt gctaatggtg ctattgaaac aatgggaatg
     1141 ttaaatatca ggaaagttac tccatttgaa tttctgaatt attcattccg tgagcctgtt
     1201 tatgtgcagc tacattccga aaattattac gaacgcaaag acgggtcaga ttttttgaag
     1261 gccgactttt atcactaccc tgaaaaatat aattgcactt tctgtgaccc gggatcatat
     1321 tcatcgttca ccgacttgtt ggtgcattgt gcctattgga atcctaatgc tgctgtgctg
     1381 tttacgaaag aagatatgcg aagccctgca ttccgtatca gtgtcattgc agatgtcact
     1441 tgcgatattg atggtagtat tccttcaacg catcgcgcaa caactattga cgataaattt
     1501 tatggttata atccaatgaa tgaaaaagag gaggaagcat tcagtacgga taccattact
     1561 gtcatgtcta ttgataacct tccttgcgaa ttaccgcgtg atgcttctga aggttttggt
     1621 aagcatttaa ttgaacgtgt tattcctctt ttgataggtg aggacaaagg caatgtgata
     1681 taccgtgcta ccatagcccg caatggtgaa ttgactccac gatttgcata tcttacggat
     1741 tacatcagtt gatgtttatc cttttttaag tacttcaatt gtggcctttg ggtcagaaga
     1801 gctaaaaacg gtatttcctg caacaagaac atcggcaccg gcagcaataa gttttttgta
     1861 gttattcagg tcaacaccac catcaatttc tataagtgtt tttgagttgt gaatagcaat
     1921 tagctctttc aactcctgaa tttttctgta cgtgttttct ataaatttct gaccgccaaa
     1981 acccggattc acgctcatca tacaaattaa gtcggcttcg aaaataatat cgctcaatga
     2041 tgccacaggc gtgtgtggat ttatggcaat acctgctttc ataccttctt tttttatctg
     2101 ctgtattgtt cggtgcagat gaacagatgc ctc
//
LOCUS       DAOOOL010000276         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6578_length_2116_cov_70.569626, whole genome shotgun sequence.
ACCESSION   DAOOOL010000276 DAOOOL010000000
VERSION     DAOOOL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6578_length_2116_cov_70.569626"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKU82_12640"
     CDS             complement(<1..793)
                     /locus_tag="PKU82_12640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOZ83757.1"
                     /translation="MRDTFEPNRLRTMINIAFSCITAFIITFLAIPSIIKVAVIKNLF
                     DEPGGRKAHKASVPTLGGLAIFAGVVFAFTFWSAGFNLQDTQYIIAALIVMFFIGIKD
                     DIVELPAMKKLYGQIVAAVIIVLFANIRITGLYGIFGIDQVPYWFSLLFSMFTILLII
                     NAFNLIDGVDGLAAGIGAIAAFTYGIYFYNYNEIALCILSFSLFSSLLAFLVYNFAPA
                     NIFMGDTGSLIIGLILSILTIKFIEMSFAPRPENFPFQSSPAMAVA"
     gene            complement(866..1867)
                     /locus_tag="PKU82_12645"
     CDS             complement(866..1867)
                     /locus_tag="PKU82_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83758.1"
                     /translation="MQFGHDKNFIGDGYRSLLLSDNANSYPFLKISTTAWKVKYVNLF
                     TVLTDLQFPAPNDIAFKKKYASFHYLDLNIGKHASFGILESVVWSGDSARGQGFDINY
                     MNPFVFIRPVEFSIGSPDNMMLGFNLKVKLFSKLVFYGQLMLDEFKLSEIRDGNGWYA
                     NKQAFQLGLKSYDLPIKNLDVLTEFNYVRPFTYSHRTTFTNYAHFNQSLAHPLGSNFY
                     ESMSLINYKYKNFIFRFQFNYAETASDTGNANLGNDIFKSYETYTFAYGNRMLQGQRH
                     NLIYSDIRAGYLINPRTNLCFEIGYSYRRMTTNSEAATTNWVYAGIRTALFNRYHDF"
BASE COUNT          719 a          402 c          385 g          610 t
ORIGIN      
        1 tggcaactgc catagccgga ctactctgaa atggaaagtt ttccggacgt ggtgcaaaac
       61 tcatttcaat aaacttgata gtgaggatag ataaaatcaa cccaatgatt aaagaacccg
      121 tatcgcccat aaaaatgttg gcaggtgcaa aattataaac caagaaagct aataacgaac
      181 tgaataatga aaacgaaaga atacatagag ctatctcatt atagttgtag aaataaattc
      241 cataagtaaa agctgcaata gcaccaatgc ctgctgccag tccgtcaaca ccatcaatca
      301 agttaaaagc attgatgata agcagaatgg taaacattga aaacaaaagg ctgaaccaat
      361 atggcacctg atctattcca aaaattccat aaagacctgt aatgcggatg ttggcaaaca
      421 agactataat aacagctgca actatctgtc cgtaaagttt tttcattgca ggaagttcaa
      481 caatgtcatc ttttattccg ataaaaaaca tcactatcaa agctgcaata atgtactgtg
      541 tgtcctgaag gttaaacccc gcagaccaga aagtaaatgc aaaaacaaca ccggcaaaaa
      601 tggctaagcc accaagggtt ggaacacttg ccttatgtgc cttacgtccg cccggttcat
      661 caaaaagatt ttttattacg gcaaccttaa taattgacgg tatggcaaga aaggtaataa
      721 taaaagcggt aatacaacta aaagcaatat taatcatggt gcgtaatctg tttggctcaa
      781 atgtatctct caatatttac tacaaaggta ctattttgcc aatagcagtt tttttcaggt
      841 aaaacaactg taaatatagg tgtaattaaa aatcatgata acggttaaaa agtgctgtcc
      901 tgatacctgc ataaacccag tttgtggtgg ctgcttcact attggttgtc attctcctat
      961 agctataacc aatctcaaaa cataagttgg tacgtgggtt aattaagtat cctgctctga
     1021 tgtcgctata aatcaggttg tgacgttgcc cctgaagcat tctgttacca taagcaaaag
     1081 tgtaggtttc gtagctcttg aaaatgtcgt tgcccaaatt tgcatttcct gtatcgcttg
     1141 cagtctcagc atagttaaac tgaaaacgga aaataaaatt tttatacttg tagttaatca
     1201 aagacatcga ctcataaaag tttgatccta aaggatgtgc cagcgactga ttaaaatgtg
     1261 catagtttgt aaaggttgta cggtgcgaat aagtaaacgg ccttacatag ttaaactctg
     1321 tcagcacatc aagattttta atcggcaaat cataactctt taatcctaac tgaaatgctt
     1381 gcttgttagc ataccatcca ttgccgtctc ttatttccga tagtttaaat tcatccaaca
     1441 tcaactgtcc gtaaaatacc aatttcgaaa acaactttac tttcagatta aaacccagca
     1501 tcatattatc tggcgaacct attgaaaact ctacaggacg aataaataca aaaggattca
     1561 tgtagttaat gtcaaaaccc tgcccacgtg ccgagtcgcc cgaccagaca acactttcta
     1621 aaattccgaa cgatgcatgt ttgccgatgt taaggtctaa ataatggaat gatgcatatt
     1681 tctttttaaa agcaatgtca ttaggtgcag gaaactgaag gtcggtgagt acggtaaaca
     1741 aatttacata cttaaccttc catgccgtag ttgaaatttt cagaaaagga tagctgttgg
     1801 cattatcaga caaaagcaaa gagcgataac catcgccaat aaaattttta tcgtgtccaa
     1861 actgcacatt aaaatgtctg ttgatggtgt atgatatagt tcctgtaacg ttggaataat
     1921 catagctgcc atattgtttt tttatgcgcc cctcaccggg aataatgttg tagcgtgtaa
     1981 caaacgtatc aatgtatgac ggaaaccttg cctgattttc gaaaaatgta gtagagaaag
     2041 aaaatctttt tcctgccgtt cctgtatgct aatgttgatg tgttgattat tgcatcggca
     2101 tttggcattg cagtgt
//
LOCUS       DAOOOL010000277         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6680_length_2090_cov_162.739066, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000277 DAOOOL010000000
VERSION     DAOOOL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_2090_cov_162.739066"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKU82_12650"
     CDS             <1..309
                     /locus_tag="PKU82_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83759.1"
                     /translation="LAGSVCDTITGIAPSPLENPDSYREGVRLYPNPANSTLYASGMP
                     EGKNEMQVYDLYGRLLLKKQNNSSSGVIDVSSLSDGVYNIRIKNETGEYFKKRFVVIK
                     "
     gene            326..1804
                     /locus_tag="PKU82_12655"
     CDS             326..1804
                     /locus_tag="PKU82_12655"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83760.1"
                     /translation="MAATLCCCHFFCFSQNKNSVWCFGDSAGIDFGSGVPVTFQSGMD
                     SRGSCVSISDNNGNLLFYAATMPYSGSNPAFSTIVFNSTNSIMANGDSIFGKGWYQEL
                     VIVPNPANDSTYYLFSIGSTTTFGLCYSIIDMRLNNGLGAVTVKNIQLQSFQQVDCLN
                     AVKHGNGRDWWVLFRKAGSLSNGNNDWYSYLITPDSIQNVSVQSIGSLNKTNSGHTNL
                     IGLTELYDFDRCSGLLSNLVTIEPEALPSVPYLFSCAFSPDASKLYVSTADQPTWLFQ
                     YDLNASNITLSKDTLWTISSPQYAGGGLKLAPDNKIYLASIYYNGVQFPYPYQDTVYN
                     MYNMNLSVINQPDSLGSACDFQPYSFYLGGKRTYLGLPNNPDYTLPAISGSPCDTLTG
                     IAPLATSAKGAELFVTYMASWQKLFVNAQHLKGKNITLTLYDVTGKLIYNTARVLSPS
                     GVGGSAGGYFTQDINCSALAKGMYIVSLQTEKERLVNKFIVE"
BASE COUNT          643 a          367 c          420 g          660 t
ORIGIN      
        1 ttagcgggaa gtgtgtgtga tacaatcaca ggtattgctc cctctccttt ggagaatccc
       61 gatagctatc gggagggagt gaggttatat cccaaccccg ccaacagtac attgtatgct
      121 agcggaatgc ctgaaggcaa aaacgaaatg caggtttatg acctttacgg caggttatta
      181 ttgaaaaagc aaaataatag cagcagtggt gtaatagatg tatcttcgct ttctgacgga
      241 gtgtataata ttagaataaa gaatgaaaca ggcgaatact ttaaaaagcg gtttgtggtt
      301 attaaataat ttaaaatgtt tactaatggc agcaacactc tgttgctgcc attttttttg
      361 cttttcacaa aacaagaatt ctgtttggtg ctttggtgat agtgcgggaa tagattttgg
      421 ttcgggagta cctgtaactt ttcaatccgg tatggatagt agaggaagct gtgtcagcat
      481 ttcagacaat aacgggaatt tactttttta tgcagcaaca atgccttatt caggttcaaa
      541 tcccgcattt tcaaccattg tttttaactc cacaaattca ataatggcta atggtgattc
      601 tatatttggc aaaggatggt atcaggaatt ggtaatagtg cctaatcctg ccaatgacag
      661 cacctattat ttattttcta ttggaagtac taccactttt ggtttgtgct atagtattat
      721 tgatatgcgg ctaaataatg gattgggtgc agttacagtt aaaaatattc aattgcaaag
      781 ttttcagcag gtggattgcc tgaatgctgt taaacatggc aatggaagag actggtgggt
      841 attattcaga aaagcaggtt ctttatcaaa tggcaacaat gactggtatt cttatttaat
      901 aacacctgac agcattcaga atgtttccgt tcagtcaata ggttcattaa acaaaactaa
      961 ctctggacat accaatttga ttggtttaac agagctatat gattttgacc gttgctcagg
     1021 attattaagc aatcttgtta ccattgaacc ggaagcgttg ccatcagtgc catatttatt
     1081 tagttgcgca ttctcacccg atgcaagcaa actttatgta agcactgctg accaaccgac
     1141 atggcttttt cagtacgatt tgaatgcatc taatattact ttatcaaaag acacgctttg
     1201 gactataagc agcccacaat atgcaggtgg cggattaaaa ttagctccag acaataaaat
     1261 ttatttagcc agcatatact ataacggagt tcaatttcct tatccatatc aggatactgt
     1321 ttacaacatg tacaatatga acttgtcagt aataaatcaa cctgatagtt taggaagtgc
     1381 ttgcgatttt cagccgtaca gtttttatct gggcggtaaa agaacgtatt taggtttgcc
     1441 taataatcct gattatacac tgcctgcaat atcaggcagt ccctgtgata cattaacggg
     1501 tatagcacct cttgcaacat cagcaaaggg agcggagttg tttgttacct acatggcatc
     1561 atggcaaaaa ttgtttgtaa atgcgcagca tttaaaaggg aaaaatataa cactaaccct
     1621 atatgatgtt acaggcaagc taatttataa cactgcgcga gtgctctccc cttcaggggt
     1681 tgggggtagc gcaggcggtt attttacaca ggatattaat tgcagcgcac ttgcaaaagg
     1741 catgtacatt gtttcgttgc aaacagaaaa agaaagattg gtaaataaat ttattgttga
     1801 ataacttttg ttttaaatga aattactcaa atacataagc gcagcatccc cgtacccgcg
     1861 aagctgtgct tcgtgggttt tgtgtttgga agttttactt caatcatata ctgatctaaa
     1921 aattatcatt tttgcatata tgcaaacaac aaatgtctga agtttataaa acttttccgg
     1981 gcggtttata ttttgtaaca tttacaacca ttggctggat tgatattttc ccccactggc
     2041 gcaagtccaa tgtcattaag ttaaggaaac aaacagctgt ctattaataa
//
LOCUS       DAOOOL010000278         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6710_length_2084_cov_40.545589, whole genome shotgun sequence.
ACCESSION   DAOOOL010000278 DAOOOL010000000
VERSION     DAOOOL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6710_length_2084_cov_40.545589"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKU82_12660"
     CDS             complement(<1..851)
                     /locus_tag="PKU82_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOZ83761.1"
                     /translation="MSLSLDQIKKPISLEMDCFENKFRDAMRSKVSLVDKIMHYIVKR
                     KGKQMRPMFVFLSAGVCGKINESTYTAASLIELLHTATLVHDDVVDDALQRRGFFSIN
                     AVWKNKIAVLVGDYLLSRGLLIAVQNKEFKLLEIVSNAVRDMSEGELLQMEKARRLDI
                     DEEVYYKIIRQKTASLIASCCSCGAASVQAGDEKVELMRELGETIGLAFQIKDDLFDF
                     EKSISTGKPSGIDIRERKLTLPIIYALSQATSSEKRTIINLIKNEHENESKVAEVLAF
                     VNRQKGI"
     gene            complement(902..1966)
                     /gene="rlmN"
                     /locus_tag="PKU82_12665"
     CDS             complement(902..1966)
                     /gene="rlmN"
                     /locus_tag="PKU82_12665"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOZ83762.1"
                     /translation="MFSSYLCKVKSIPNIRELTFEQLSEAVADFGEKSFRAKQIYDWL
                     WNKSARSFDQMNNVPLSLRNKLAEKFAFTPIVVAHEQRSNDRTIKYGFKLHDDHLIEG
                     VLIPTASRMTACVSSQVGCSLSCKFCATGKLDRVRNLNADEIYDQVAIIREQSMEVYN
                     IPLSNIVYMGMGEPLLNYANVLASIERITSANGLGMSPQRITVSTAGIAKMITKLGDD
                     EVKFNLALSLHAANDSKRDKIMPINEHNNLEVLRDALKYFYDKTGTRVTYEFIVFNNF
                     NDTLKDAQELAEFAKHVPCKINLIEYNAIGDGVFEKARLDRLEVFKNYLESKNLVVNI
                     RRSRGKDIDAACGQLANKNQ"
BASE COUNT          623 a          437 c          347 g          677 t
ORIGIN      
        1 tcaatacctt tttgcctatt gacgaatgcc aaaacttcag caaccttact ttcattctca
       61 tgttcatttt taatcaggtt gatgattgtt cttttttctg aggaagtagc ttgtgacaat
      121 gcataaataa tcggcagtgt aagttttctt tcacgaatat caatgcctga aggtttacct
      181 gtagagatac ttttttcaaa atcaaaaagg tcgtctttaa tctgaaaagc taaccctatt
      241 gtctctccca attcgcgcat aagttcaacc ttttcatcac ctgcctgtac agatgctgca
      301 ccacaactac aacaagaagc aattaatgat gctgttttct gacgaataat cttatagtaa
      361 acttcctcat caatatcgag tcttcttgcc ttttccattt gcagcagttc accttcactc
      421 atatcacgca ctgcattact cacaatttcc aaaagtttga actccttatt ctgaacagca
      481 ataagtaacc cacgtgacaa taaataatcg cctaccagca ctgcaatttt atttttccag
      541 actgcgttaa tagaaaaaaa acctctgcgc tgtaaagcat catcaacaac atcgtcatgc
      601 accaaagttg cagtgtgcaa taattcaata agagatgctg ctgtgtatgt tgattcattt
      661 atttttccgc atacaccggc tgaaagaaaa acaaacatgg gtcgcatttg cttgcctttg
      721 cgcttaacaa tgtagtgcat gattttgtca accaacgaca ctttgctgcg catggcatcg
      781 cgaaacttgt tttcaaagca gtccatttcg agcgaaatgg gctttttaat ctgatctaaa
      841 ctgagcgaca taataaacgg ctgcaaaggt atgtttttaa gtagcagcag cctttggcag
      901 tttactgatt cttattagct aattgaccac atgcagcatc aatatcttta cccctgctgc
      961 gtctgatatt gactactaaa ttcttgcttt ctaaataatt tttaaaaacc tctagtctgt
     1021 ccaatcgagc cttttcaaaa acaccatcac ctattgcatt gtattctatt aagttaattt
     1081 tacaaggaac atgttttgca aattcagcta attcttgggc atctttaagt gtgtcgttaa
     1141 aattgttaaa aacaataaac tcataggtta cacgtgtgcc tgttttatca taaaaatact
     1201 tcagggcatc acgtaaaacc tcaagattat tatgttcatt gatgggcata attttatcac
     1261 gcttactgtc attggcagca tgtaatgata gcgcaagatt aaactttact tcatcatcac
     1321 caagttttgt aatcatcttt gcaattccgg ctgttgaaac tgtaattcgt tgtggcgaca
     1381 taccaagtcc gttagcagat gtaattcttt ctatggatgc aagcacattg gcataattta
     1441 aaagaggctc acccattccc atataaacta tgttgcttaa tggaatatta tagacctcca
     1501 tactttgttc tctgataata gccacctgat cataaatctc atcagcattc agattgcgta
     1561 cccgatctaa tttgcctgtt gcacaaaatt tgcatgacag actgcaacca acctgagagg
     1621 aaacacaagc agtcattcgt gacgcagtgg ggattaaaac accctcgatt aaatgatcgt
     1681 catgcagttt aaatccatac ttaattgttc tgtcgttact tcgttgttca tgtgcaacaa
     1741 caataggagt aaatgcaaac ttctctgcca gtttatttcg gagtgacaac ggcacattgt
     1801 tcatttggtc aaatgaacgt gcacttttat tccagagcca gtcgtaaatc tgtttagctc
     1861 tgaatgactt ttcgccaaaa tcagcaacgg cttcagaaag ttgttcgaaa gtaagctccc
     1921 ttatgttagg tattgatttc actttgcaaa ggtacgatga aaacatcagc cgatacacaa
     1981 atcacttagt tttgtaagca tgaaatttta ttcggcagat attgttttcc ctatttgcag
     2041 tactccgctt aaggatggta tagttgtaat ggacaattca ggag
//
LOCUS       DAOOOL010000279         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_6771_length_2071_cov_50.318948, whole genome shotgun sequence.
ACCESSION   DAOOOL010000279 DAOOOL010000000
VERSION     DAOOOL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_2071_cov_50.318948"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PKU82_12670"
     CDS             complement(<1..1455)
                     /locus_tag="PKU82_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOZ83763.1"
                     /translation="MINLQNTFPLFSKKLINAMFIMPLVFLMLFVSNVWAGGNDTLSA
                     NIDENVHVAEKPKDFEIALQPALLSDNSTSQIRAGLIYDCTTNTIVWEKDLKYAYPIA
                     SLTKMMVALIAIEEIKCGTVDWQDQVSVTKTYRKSKRSKRTYTVNETYSLESLLKMAM
                     IPSNNEACGLIGKHISGSVETFVTRMNQKAAALGMTQTFYSNPSGLPAGYGSLDNSSS
                     PYDLLKLSTELIKYPELLSITNIAFAEVENGHGYNVYRNHNHLVMDYPGEVDGLKTGY
                     TKNAKFCLVATAKKNDHRLIAIALGASNPWLRNQIVGEMLNNYYTQLGFGPMGNQKVD
                     PLFAKADRKTVNEIASSNVPVMNQEIVYKTVTSIQKKAHIVKSGQTLSDIADKYNCSV
                     NELKKWNGLKSSRILKNQRLYVRVHVKKQIPVSTKVIDNYDDCEDNAEDCAPQNVAGK
                     QETPKTEQLPTSQNKPALKVIYHQVQPGDTLWKIA"
     gene            complement(1641..>2071)
                     /locus_tag="PKU82_12675"
     CDS             complement(1641..>2071)
                     /locus_tag="PKU82_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOZ83764.1"
                     /translation="RSVFNGLQTIEDREALVAVHDAARPLLKPELAEKLFELAVHRGN
                     AIPVIPVRESLRRINSTENKAVNRAEIFAVQTPQVFKVDLLKDAFIRNNDTTFTDEAS
                     LIEAVGFTVFSIQGDRHNIKITFPEDILVASMLLKKHLSN"
BASE COUNT          602 a          393 c          363 g          713 t
ORIGIN      
        1 tgcaattttc cataaggtat caccgggctg tacttgatga tatataacct tcaatgcagg
       61 tttattttgt gatgttggca gttgttctgt tttaggagtt tcctgcttgc cggcaacatt
      121 ttgtggagcg caatcttctg cattgtcttc gcaatcatca taattatcaa taacctttgt
      181 gctgacagga atttgtttct ttacatgcac ccttacataa agtctttgat ttttaagaat
      241 ccggcttgac tttagtccat tccatttttt aagttcatta acagaacaat tatatttatc
      301 agctatgtca gataaagttt ggcctgattt tacaatgtgt gcttttttct gaattgaagt
      361 cactgtttta taaactattt cctgattcat cacaggcaca ttactgcttg caatttcgtt
      421 tacagttttt ctgtctgctt tagcaaacaa tggatcaact ttttgatttc ccataggccc
      481 aaagcccaat tgtgtgtaat aattattgag catttcgcca acaatctgat tgcgcaacca
      541 agggtttgat gcacctaatg caatggcaat taagcgatga tcgtttttct ttgcagtagc
      601 aaccaaacag aattttgcat tttttgtata tcctgttttt agtccgtcca cttcaccggg
      661 atagtccata actaaatgat tatggtttct gtaaacatta tatccatgcc cgttttcaac
      721 ttctgcaaaa gcaatattgg taatggaaag aagttcggga tatttgatta attcagttga
      781 cagctttagc aaatcgtaag gtgaagagct gttgtcaagg cttccatagc ctgcaggtaa
      841 tcctgatgga tttgaataga aagtttgtgt cattcctaat gctgctgctt tctgattcat
      901 tcttgtcacg aaagtttcaa cagagccact gatatgtttt cctattagtc cgcaagcttc
      961 gttatttgaa ggaatcatag ccatctttaa taatgactca agactataag tttcgtttac
     1021 ggtataagta cgcttgcttc ttttactttt tctgtatgtc tttgtcacag aaacctgatc
     1081 ttgccaatca acagtaccac atttaatctc ttcaatggca ataagggcaa ccatcatttt
     1141 tgttagtgaa gctataggat aggcatactt taaatctttt tcccatacaa tggtatttgt
     1201 tgtacaatca taaatgagtc ctgcacgaat ttgcgaagtg ctgttatcac tgagtaaagc
     1261 aggttgtagc gcaatttcaa aatctttagg cttctctgca acatggacat tttcatcaat
     1321 attagcagaa agtgtatcgt tgcctccagc ccaaacattg gacacaaaca acatcagaaa
     1381 taccaatggc attataaaca tagcatttat gagttttttt gaaaacaatg gaaaagtgtt
     1441 ttgcaagttt atcatttaat gctttgaatt ttttattgct ccaacaagtg atttacacaa
     1501 cccgatgttg atagtactta aacgtaaaaa actttgctat cgtttctttc aacatcaaat
     1561 atttttttca atgtaacgat ttgcgcaata aaattaaaca aatttttata aattcttaca
     1621 agagaaaata agtgcctgct tcagttggat aaatgcttct tgagcagcat ggatgcaaca
     1681 agaatgtctt ccggaaaagt gatttttata ttatgtctat cgccctgaat ggaaaataca
     1741 gtaaacccaa ctgcttcaat cagcgaagct tcatcagtaa aagtggtatc gttatttctg
     1801 ataaaagcat ccttaagcaa atcaacttta aagacctgcg gtgtttgtac agcaaaaatt
     1861 tcagccctgt taacggcctt attctccgta gaattaatac gtctcagcga ctctctgaca
     1921 ggtataacgg gtatggcatt gcccctgtgg acagcaagtt caaagagttt ttcagcaagt
     1981 tcgggcttta aaagtggccg tgctgcatca tgcactgcaa ccaaagcttc tctatcttca
     2041 atggtctgta aaccgttaaa taccgaacgg t
//
LOCUS       DAOOOL010000280         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7041_length_2018_cov_83.495670, whole genome shotgun sequence.
ACCESSION   DAOOOL010000280 DAOOOL010000000
VERSION     DAOOOL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7041_length_2018_cov_83.495670"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2018)
                     /gene="rpoB"
                     /locus_tag="PKU82_12680"
     CDS             complement(<1..>2018)
                     /gene="rpoB"
                     /locus_tag="PKU82_12680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOZ83765.1"
                     /translation="QLINSKADVDDIDHLSNRRVRTVGEQLYSQFGVGLSRMARTIRE
                     RMNVRDNEVFTPADLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRLSALGP
                     GGLSRERAGFEVRDVHYTHYGRLCTIETPEGPNIGLISSLCVFAKINNLGFIETPYRV
                     IENGKVDLNRPPVFLTAEEEDNKVIAQANAHIDATGNFLDARVKARYEGDFPEVDPEH
                     LHLMDVAPNQIASIAASLIPFLEHDDANRALMGSNMQRQAVPLMRPEAPVVGTGLEGQ
                     VVRDSRVLINAQHDGVVEYVDANEIRVRSILSEDEKLLSFSDEIEVYNLIKFQKTNQS
                     TCINLKPIVRKGEKVRAGQVLCEGYATKDGELALGRNLKVAFMPWKGNNFEDAIVISE
                     RVVREDIFTSLHVDEYTLEVRDTKRGLEELTADIPNVSEEATRDLDDNGLIRVGAEVK
                     EGDIIIGKITPKGETDPSPEEKLLRAIFGDKAGDVKDASLKLPPSVKGVVIDKKLFSR
                     AVKDKNSKTDDKVVLTKLDDEQARDLSAIKAKLVDKLFILVNGKTSQGVMDIFKEFVI
                     PKGSKFTQKMLQELDYNNINPSKWTTDKDRNDQIKELLHNYNIKVNDVVGSYKRKKFA
                     IQVGDELPAGIMQLAKVYIAKKRKLTVGDKMAGRHGNKGIVAKIVREE"
BASE COUNT          555 a          465 c          362 g          636 t
ORIGIN      
        1 tcctcttcac ggacaatctt agcaacaata cctttattac catgacggcc tgccatctta
       61 tcacctactg taagtttacg ttttttagca atgtaaactt ttgctaactg cataatacct
      121 gctggcagtt catcaccaac ctgaatagca aacttcttac gtttatagga acctaccaca
      181 tcatttactt taatgttgta gttgtgcaac aactctttta tctgatcatt acgatcttta
      241 tcggttgtcc attttgatgg gttgatgtta ttataatcca actcctgcaa catcttctga
      301 gtaaacttgc tgcctttcgg aataacaaat tctttaaaga tatccatcac accttgcgat
      361 gtttttccat taacgaggat aaacagtttg tcaaccaatt tagcttttat agctgataga
      421 tctcttgcct gctcatcatc aagtttggtt aacactactt tatcatcagt ctttgagttt
      481 ttatctttca ctgcacgtga aaacaacttc ttgtcaatta caacaccttt tacagatggt
      541 ggcagtttca gtgatgcatc tttaacatca ccggctttat caccaaagat ggcacgaagc
      601 agtttctctt cgggtgaagg atctgtttct ccttttggtg tgatttttcc gataatgata
      661 tctccttctt tcacttctgc accaacacga atcaatccgt tatcatcaag gtcgcgggtt
      721 gcttcttcac taacgtttgg aatatctgca gtaagttctt caagaccacg cttggtgtca
      781 cgaacttcaa gagtatattc gtcaacgtgc agcgaagtga aaatatcttc gcgtacaaca
      841 cgctcagaaa tcacaatagc atcttcaaag ttatttcctt tccagggcat gaaagcaact
      901 ttaaggttac gtcctaaggc caattcaccg tctttggttg cataaccttc gcacaatacc
      961 tgtcctgctc taaccttctc cccttttcta acaataggct taaggttaat acaagtgctt
     1021 tgatttgtct tctggaattt tatcagatta taaacttcaa tctcatcact aaagctcaac
     1081 agtttttcat cttctgaaag aatgctgcga acacgaattt cattggcatc tacatattca
     1141 actaccccat catgttgcgc attgatgagc acacgtgagt cgcgcaccac ctgaccttca
     1201 agtcctgttc caacaacagg ggcttccggg cgcatcaacg gcacagcctg ccgttgcatg
     1261 tttgaaccca tcagtgcacg gtttgcatca tcatgttcaa ggaaaggaat caatgatgct
     1321 gcaatagatg caatctgatt aggtgctacg tccatcaggt gcagatgctc aggatctact
     1381 tcagggaagt caccttcata acgtgcttta acacgtgcat caaggaagtt tcctgttgca
     1441 tcaatatgtg catttgcctg tgcaattact ttattatctt cttcttctgc agttaaaaat
     1501 acaggaggac gattcaaatc cacttttccg ttttcgatta cacgataagg tgtttcaatg
     1561 aatccaagat tgttgatttt tgcgaaaaca cagagtgaag aaatcaaacc gatgtttggt
     1621 ccttcaggtg tttcaatggt acacaaacgt ccgtaatgtg tatagtgaac gtcacgtacc
     1681 tcgaaacctg cacgctcgcg cgaaagacct ccgggtccaa gggcagagag ccttctttta
     1741 tgcgtaatct cagccaacgg atttgtttga tccataaact gtgacaactg attggttccg
     1801 aagaatgaat tgattactga agacaatgtt tttgcattaa tcaaatcagc aggcgtaaac
     1861 acttcgttgt cgcgaacgtt catacgctca cggatggtac gagccatacg cgataaacct
     1921 acaccaaact gagaatataa ttgttcgcca actgtacgta cacgtctgtt tgataagtga
     1981 tcaatatcat ccacatcagc tttagagttg ataagctg
//
LOCUS       DAOOOL010000281         2016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7051_length_2016_cov_266.906170, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000281 DAOOOL010000000
VERSION     DAOOOL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7051_length_2016_cov_266.906170"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1375
                     /locus_tag="PKU82_12685"
     CDS             314..1375
                     /locus_tag="PKU82_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83766.1"
                     /translation="MLFVIDEMAGVHPAVLTAIINTCTAPNNLIIGVGNPDSQIDTLH
                     TFCQLKKTEHIIVSAYDHPNVVNKRMIIPGAVSVESIDMRKEEYGEESNFYKSRVRGI
                     APEEGTGSLFRAEYIDQCYIYNKDKFHGNPEKIGNNAVGVDVANSEQGDMACLAWGKG
                     NKLFQLQEFQCDNATHLAYNMMYDTHELARFGYKNYNTLKIKDYKIHDRNIGIDTVGV
                     GVATINAFYDKRKDVVALIGGQLQEALNLDNDGNPLYNFQNLRSQMYFEFREDLRTNG
                     VILDLDKKTFLKLKKELLAHTYKVSGGKIFVESKDDIKKKLGGKSPNLSDSAVYWNFV
                     RKNYYKKTMSLPFSGSAKK"
     gene            1405..>2016
                     /locus_tag="PKU82_12690"
     CDS             1405..>2016
                     /locus_tag="PKU82_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83767.1"
                     /translation="MGFLDDFGNIVLRYAFGEHKNNQAQTTAKPTVIKAKTEPGFMPV
                     GNVRSSVESGASTFAPTGKLQEIKPDFAIEYLSTIENLAAFNPDISYGLDNIVTLGNT
                     EHDIYFSDKIPESKQSEMRNYLLEQERTWYGFSGGVRSLKGDLLTQIVINGALSAEAV
                     PREDLTAIKKIVRVSPKHIRFIYDKEADEFYPYQNVNILSKASS"
BASE COUNT          666 a          349 c          460 g          541 t
ORIGIN      
        1 acgcatcatt tattggtttt tagacacgtt cccaaactcg cttgtaatta ctactgctcc
       61 gaaaaaagac caactaaaaa agattctatg gacggaaatg gggaatgcat accataagtt
      121 caaaaagata cggccacact ccgaatttat taccctgaat attgcagttg ataagcgtac
      181 caaaaaaacc tccacacaga aagttgatga tagcattgag ggaggaatgg gttgggaggt
      241 attaggtatc gtttccggtg tggccgcagg tgaggattca gcgaccaaaa tgcagggttt
      301 tcacagggag aatatgttat ttgtcattga cgaaatggcc ggtgtgcatc ctgcagtatt
      361 gacggcaatc attaatacgt gtacggcacc aaataacctg attataggcg taggcaatcc
      421 cgatagccaa attgataccc tgcatacgtt ctgtcagttg aaaaaaacag agcatattat
      481 tgtatctgca tacgaccacc cgaacgttgt gaataagcga atgataatac caggtgcagt
      541 atcagttgag agtatcgaca tgagaaagga ggaatacgga gaggaaagta acttctacaa
      601 aagtcgtgta cgtggtattg ctccggagga gggaaccggc tcattattca gagcggagta
      661 tatagaccaa tgctacatat acaataagga taagtttcat ggcaatcctg aaaagattgg
      721 taataatgcc gtcggagtgg acgtagcaaa cagtgagcag ggtgacatgg cttgtttagc
      781 atggggtaag ggtaacaaat tatttcagtt gcaggagttc caatgtgata acgccacaca
      841 tcttgcgtat aacatgatgt acgatacgca tgaattggca cgttttggtt ataagaacta
      901 caataccctg aagattaagg attacaagat acacgaccgt aatatcggta ttgatacggt
      961 aggtgttggt gtagctacta tcaatgcatt ctatgacaaa agaaaagatg tggttgcact
     1021 tatcggaggc caacttcagg aggctttaaa tttggataat gacggcaatc cgctttataa
     1081 ctttcagaac ttacgttctc aaatgtactt tgagttcagg gaagatttgc gtaccaatgg
     1141 cgtaatttta gatctcgaca aaaagacatt cctgaaactt aaaaaagaac tgttagcaca
     1201 cacatacaaa gtttccggag gtaagatatt tgtggaatca aaggacgata ttaaaaagaa
     1261 actcggtgga aaatctccga acctatcaga tagtgcagtt tattggaatt tcgttcgtaa
     1321 aaactactac aaaaaaacta tgtcattacc gttttcaggt agcgcaaaga aataatttat
     1381 acttaacttt aggaaaagta taacatgggt tttctcgacg atttcggaaa tatagtattg
     1441 aggtatgcgt ttggtgagca taagaataac caagcacaaa ctactgcaaa gcctactgtt
     1501 attaaggcta agacggagcc gggatttatg cctgttggta atgtcagaag tagtgtagaa
     1561 tcgggtgcct ccacgtttgc accgaccgga aaattgcagg aaataaagcc tgattttgct
     1621 attgaatatc tatctaccat tgaaaatttg gccgcattca atccggatat ctcttatgga
     1681 ttagacaaca ttgttacgtt gggtaataca gagcacgata tttacttctc cgataagata
     1741 cctgaaagta agcaatctga aatgcgtaat tacctattag agcaggagcg aacgtggtac
     1801 ggatttagtg gaggagtgag aagtttgaaa ggagatttac tcacacagat tgtgataaat
     1861 ggtgcactat ctgcagaggc cgttccacga gaagatttaa ctgccattaa gaaaatcgtt
     1921 cgtgtatctc caaagcacat acgtttcata tacgacaaag aggccgatga attttatccg
     1981 tatcagaatg taaatatttt gagtaaagcg agtagt
//
LOCUS       DAOOOL010000282         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7241_length_1979_cov_35.380977, whole genome shotgun sequence.
ACCESSION   DAOOOL010000282 DAOOOL010000000
VERSION     DAOOOL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7241_length_1979_cov_35.380977"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1979
                     /locus_tag="PKU82_12695"
     CDS             107..>1979
                     /locus_tag="PKU82_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83768.1"
                     /translation="MPLKHRLLLFLLLMIGFGYDANAKHIAGGDFTYRRLNGNNFEIT
                     LKIFRDCSDFVAFDNAILIGVFNKGTNTLINSYTVALTDSGSLTLTGTSCLTPPTGVC
                     MDKCTYVDTISLPNNPSGYYVSWERCCRNNAIVNLTRPGDTGIAYYMEIPDPAIIDNS
                     PNFNNDPFPFMCLGQDFDFDYSAYDVDGDLLTYSLETPLAGELGYPLSSTISPVWSNP
                     TPGPYQAVQWLPGYSVANITGSFPALSLNSSTGLMKVKANKVGFYAMAVVIKEFRNGV
                     QIGQIRREIEFTVINCAANAAPQISYQNSTAPQSGLTFTIYEKDSLCFTVRVTDSDSI
                     KLSYSGDIFAGGSISPPYAVTSAASGNGVATSSFCWYTSCDQGRTQPYSVTFKATDNG
                     CPQPFTSTDSLKIYVLPVPQITAPLIICMNTATEGTIKLTFADTFAMPRFIDRYVVFR
                     SANGGPFINIGNINNPTNHFYNDTTAANLDLNNYCYYIAAVNSCNETGAHSDTICSDT
                     QKNIDAPFIFSAQVQNNNSIVLNFNNPPDNLFTLFKIEKRENTFSAIYTDYFQQQGFL
                     IPQWTDSLQNVTEKSYCYRITKKNQCDIESPVSNESCSILLTGQSLPLQHILNWTPYT
                     "
BASE COUNT          612 a          401 c          332 g          634 t
ORIGIN      
        1 ctattagtac aacttcttag tgtgcatttt ttgtcatttc acccaaatta atatcctgac
       61 aatgacgtat tttactattt ttgctataat taagaaattc cgtttgatgc cgttaaaaca
      121 tcgtttactg ttattcttgc tcctcatgat tggttttggt tacgatgcca atgcaaaaca
      181 tattgctggt ggcgatttta cttacagacg actcaatggg aataactttg aaattacact
      241 taaaattttt cgtgactgct ctgattttgt agcttttgac aatgctattt taattggtgt
      301 atttaacaaa ggaaccaata cgcttatcaa ttcttatacc gttgcactta ctgactcagg
      361 atcactgaca ttaacaggga cttcatgtct gactccacca acaggtgttt gtatggacaa
      421 atgtacttat gtagatacta tttcactccc caacaaccca agtggatatt atgtgtcgtg
      481 ggaacgttgt tgtcgtaata atgctatcgt aaatctaact cgacccggtg atacaggcat
      541 tgcatattat atggaaattc ctgacccggc aattattgac aactctccca actttaataa
      601 cgacccattt ccatttatgt gtctaggcca ggattttgat ttcgactata gtgcctatga
      661 cgttgatggc gacttactga cttatagtct tgaaacacct ttagccggtg agttaggtta
      721 tcctttaagt tcaactattt ctcctgtttg gtcaaatcct actccaggac cttatcaggc
      781 agtacaatgg cttccgggat attcagtagc taacattaca ggaagttttc cggctttgtc
      841 tttgaatagc agcacaggtc taatgaaagt aaaagcaaac aaagtaggct tttatgcaat
      901 ggctgttgta attaaagaat tccgaaacgg agttcagatt ggtcaaataa gacgtgaaat
      961 agaatttaca gtaataaact gtgctgccaa tgcagcacct caaatcagtt atcagaactc
     1021 aactgctccg caaagcggat taacgtttac aatttacgaa aaagactctt tatgttttac
     1081 agttcgtgtt acggattccg attcaattaa actttcatac agcggtgata tttttgcagg
     1141 tggtagtatt agcccacctt atgctgtaac atctgcagct tcaggcaatg gcgttgcaac
     1201 aagttcattt tgctggtaca catcatgcga tcagggtcgc acacaaccat acagtgtaac
     1261 attcaaagca acagataatg gttgcccaca accctttaca tctactgact cattaaagat
     1321 ttatgttctt ccggttccac aaattactgc accactcatc atttgtatga acactgccac
     1381 agaaggaact attaaactga ccttcgcaga tacttttgca atgccaagat ttattgatcg
     1441 ttatgttgtt ttcagaagtg ctaatggtgg cccatttata aatatcggaa atattaacaa
     1501 tcctacaaat catttttaca atgatactac tgctgcaaat cttgacctga acaattactg
     1561 ttattacatt gctgctgtaa atagttgtaa tgaaactggt gcacattcgg acaccatttg
     1621 ctccgatacg caaaaaaata ttgatgcccc attcatcttt agtgctcaag ttcaaaacaa
     1681 caatagcatt gtattgaatt tcaacaaccc acctgataat ttattcactt tgtttaaaat
     1741 tgaaaaacgc gaaaacacat tttcggcaat atatacagac tactttcagc aacaaggatt
     1801 tttaatacca caatggacag actcacttca aaacgtcact gaaaaatcat attgctaccg
     1861 tatcacaaaa aagaatcagt gtgatattga aagtcctgta agtaatgaat catgctccat
     1921 tttgctcaca gggcaaagtt taccattaca acatatttta aactggacac cttatacag
//
LOCUS       DAOOOL010000283         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7302_length_1967_cov_37.248954, whole genome shotgun sequence.
ACCESSION   DAOOOL010000283 DAOOOL010000000
VERSION     DAOOOL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7302_length_1967_cov_37.248954"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1151)
                     /locus_tag="PKU82_12700"
     CDS             complement(174..1151)
                     /locus_tag="PKU82_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOZ83769.1"
                     /translation="MNVLVYIETAEGKLKKSATEVLSYGKAIAKNSGGMLTALAIGDL
                     AADEQSKAGRYGADKVLHVTNDKLKQFINQAFASVVAEAAKQSNTSVVVISNTFNGKA
                     MAPRVAVKLKAGMVDGAVGLPDLNNGFKVRTSAFSGKAFADIELTSAIKVITVSPNSF
                     KAEENQAAGTIEAFSPSVADSDFTAMVKEIVKASDKISLPEAEIVVSAGRGLKGPENW
                     GMIEELATLLGAATACSKPVSDAHWRPHSEHVGQTGLAISPNLYIAIGISGAIQHLAG
                     VSSSKTIVVINKDPEAPFFKAADYGIVGDAFEVIPKLIEEVKKFKAANG"
     gene            complement(1177..1914)
                     /locus_tag="PKU82_12705"
     CDS             complement(1177..1914)
                     /locus_tag="PKU82_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOZ83770.1"
                     /translation="MKILVCISHVPDTTTKISFTADNKSIATSGIQYIINPYDEIALT
                     RALELTEKSGGTVTVINVGDASTDATLRKALAIGATDAVRVDANPANASFVAAQIADY
                     ASKNSFDLILTGRESIDYNGSQVAAMVGELLNIPSVSIVKSLEVADKNVTLEREIEGG
                     KEIVSCPTPLVASATEGMAEPRIPNMRGIMSARTKPLQVMAAVATETYQQLVGYDKPE
                     PRTQVKLIDASNVKALVDALHTEAKVI"
BASE COUNT          580 a          420 c          330 g          637 t
ORIGIN      
        1 gctatagcct gcgcttcata accgccaatt attataggta gccttcgtgt gccaccttgc
       61 tcattcaata ccaaggcata ggcgccactt tgtgcctgac tataagacaa accgaaaata
      121 tcgagtctta tttttttcat caaggtttaa aagtaaacaa acttttaata tgattagcca
      181 ttagcggctt taaatttttt aacctcttca ataagttttg gtatcacttc aaaagcatca
      241 ccaacaatac cataatcggc agctttaaag aatggtgctt ccggatcttt attgataaca
      301 acaatggttt ttgatgaact tacacctgca agatgttgaa tagcacctga aatgccaata
      361 gcaatatata aattaggact gatagctaac cccgtttgcc ctacgtgctc actatgcgga
      421 cgccagtgtg catcactaac aggcttagaa catgctgttg cagcacctaa taaagttgca
      481 agttcttcta tcattcccca gttttccggt ccttttaaac cacgacctgc tgaaactaca
      541 atctcagctt caggtaatga tattttatct gatgctttta caatctcttt taccattgca
      601 gtaaaatcac tgtcagcaac agatggacta aatgcctcaa ttgtgcctgc agcctgattt
      661 tcttctgctt taaaactgtt tggagaaaca gtaattactt ttatggccga tgtcaactca
      721 atgtcagcaa atgctttgcc tgaaaaagct gaagttctta ccttaaaacc attatttaaa
      781 tcaggtaaac caacagcgcc atcaaccata cctgctttaa gtttaacggc aacacgtggt
      841 gccatagcct ttccattaaa tgtatttgaa attactacaa cagatgtatt gctttgtttt
      901 gctgcttctg caactactga agcaaaagcc tgatttataa attgctttaa tttatcattc
      961 gtaacatgta aaactttatc agcaccatag cggcctgctt tgctttgctc atcagctgcc
     1021 aaatcaccaa tagcaagagc cgttaacatc ccacctgaat ttttggcaat tgccttacca
     1081 taacttaaaa cttccgttgc cgattttttc agttttcctt ctgcagtttc tatatataca
     1141 agaacattca tatcttaaat tctcaaattt ttatttttaa ataacttttg cttctgtgtg
     1201 taatgcatca acaagtgctt ttacatttga tgcatcaatt aattttactt gtgtgcgtgg
     1261 ttcaggttta tcatatccta ccaactgctg ataggtttca gttgcaacag cagccataac
     1321 ctgcaaaggc tttgtgcgtg cactcataat tccgcgcatg ttgggaatgc gtggctctgc
     1381 cattccttct gttgcactgg caacaagtgg agtagggcat gaaacaattt ctttaccacc
     1441 ttctatttcg cgctcaaggg taacattttt atcagctact tcaagagatt ttacaatgct
     1501 tacagaaggg atgtttaaaa gttcaccaac cattgctgca acttgcgacc cattgtaatc
     1561 aatagattca cgaccggtca agattaaatc aaaactattt tttgatgcat agtctgcaat
     1621 ttgtgcagca acaaaagaag cattggccgg attggcatca acacgcactg catctgttgc
     1681 accaatagct aaagctttgc gcaaggtagc atcagtactt gcatcaccaa cattaattac
     1741 agtaacggtt ccgcctgatt tctcagtcag ttccaatgca cgggtaagtg caatttcatc
     1801 ataaggatta ataatgtatt gtatgcctga agtagcaata gacttattat cggcagtaaa
     1861 acttattttg gttgttgtgt cgggtacgtg actgatacaa actaaaattt tcatatttaa
     1921 aatgattaac tacttttttc ggaatgcaaa aataggtatt attgtac
//
LOCUS       DAOOOL010000284         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7546_length_1921_cov_43.760986, whole genome shotgun sequence.
ACCESSION   DAOOOL010000284 DAOOOL010000000
VERSION     DAOOOL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7546_length_1921_cov_43.760986"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKU82_12710"
     CDS             <1..1006
                     /locus_tag="PKU82_12710"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOZ83771.1"
                     /translation="PYSVRPEIAVEDNLQARSFLGFEVKPENDYLKSRKVLFVNDAMH
                     IGLASPTKGTTDYFYKNADADEMLFVHRGSGTLKTLFGTIDFEYGDYLIIPRGIIYQI
                     NFTTADNKLLFIESHAPIETPARYRNQYGQFMENSPFCERDFKLPHSLETHDERGDFL
                     INIKKRGLIYPYIYETHPFDAVGFDGCFYPYGISIFNFEPITGRIHMPPPIHQQFQSS
                     NFVVCSFVPRLYDYHPQAIPAPYHHSNMDSDELLYYVDGDFMSRNNIRQGQITLHPGG
                     LSHGPHPGAIERSIGKKETNELAVMIDPFNPVKITQAATEIEIEEYYQSWRMHESETI
                     "
     gene            1095..>1921
                     /locus_tag="PKU82_12715"
     CDS             1095..>1921
                     /locus_tag="PKU82_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOZ83772.1"
                     /translation="MATNDLTTVKNFNYSENKKIFEQAQDFLPLLGTDYVEFYVGNAK
                     QAAHYYKTAFGFQDLAYAGLETGVRDRTSYVLQQGKIRLVLTTPLTSSNPIADHIKKH
                     GDGVKVIALWVDDATDAFEQTVKRGAKPYLSPEVLKDKDGEVRRSGIHTYGDTIHIFI
                     ERKNYKGIFLPGYEKYQSDYNPPSTGLKYIDHMVGNVELGGMNVWSKFYAEVMGFFNL
                     ITFDDKDISTEYTALMSKVMTNGNGYIKFPINEPAIGKKKSQVEEYLDFYEGAGCQH"
BASE COUNT          626 a          364 c          383 g          548 t
ORIGIN      
        1 gccatatagt gttcgaccgg aaattgcagt tgaagataac ttgcaggcaa gaagtttttt
       61 aggctttgaa gtaaaacccg aaaacgatta tctgaagagc agaaaagttt tattcgtgaa
      121 tgatgctatg catatagggc ttgcatcacc aacaaaaggt acaacagatt acttttataa
      181 aaatgccgat gccgatgaaa tgcttttcgt tcaccgtgga agcggaactt taaaaacatt
      241 attcggaacc atagattttg aatatggcga ctaccttatt attccgcgtg gtatcatcta
      301 tcagataaat tttaccactg ccgacaataa attgttgttc attgaatcgc atgcacccat
      361 tgaaacacct gcacgttatc gcaatcagta tggtcagttt atggaaaatt cgccattctg
      421 cgaacgtgat tttaaattgc cccattctct cgaaacgcat gatgaaagag gtgactttct
      481 aataaacatt aaaaaacgcg gattgattta tccatacatt tatgaaacac acccgttcga
      541 tgcagtaggc tttgacggat gtttttatcc ctatggtatt tcaatcttta actttgaacc
      601 cattacagga cgcatacaca tgcctccgcc tatacatcag caatttcagt cgagtaactt
      661 tgtagtgtgc tcctttgtgc cgcgccttta tgattatcat ccgcaggcaa ttcctgcacc
      721 ttatcatcac agtaacatgg acagcgatga gttgctttat tatgttgatg gtgattttat
      781 gagtagaaac aatatcagac aaggacagat aacactgcat ccgggtggac tatcacatgg
      841 cccgcatccg ggagctattg aacgcagtat tggtaaaaaa gaaaccaatg agttggctgt
      901 aatgattgat ccgtttaatc cggtaaaaat tacacaggca gcaacagaaa ttgaaataga
      961 agaatattat cagtcgtggc gcatgcacga gtctgaaaca atataaagaa gcaacatacc
     1021 cctgctcctg ctctctcatt tttcacctcc accaaggaga gcagagcttc gggggtattt
     1081 aaatcaaaca accaatggca acaaatgatt taactaccgt aaaaaatttc aactattcag
     1141 aaaacaaaaa aatatttgaa caggcacagg attttcttcc actgttggga acagactatg
     1201 tagagtttta tgtaggcaat gctaaacagg ctgcgcatta ttataaaaca gctttcggtt
     1261 ttcaggatct tgcctatgca ggacttgaaa ccggtgtgcg cgacagaaca tcttatgtgc
     1321 ttcaacaagg aaaaattcga ttggtactaa ctacaccttt aacttcttct aatcccattg
     1381 ccgatcacat caaaaaacat ggcgatggtg ttaaagtaat agcgctttgg gttgatgatg
     1441 caactgatgc atttgaacaa acggtgaaac gtggtgctaa accatatttg tcacccgaag
     1501 ttctaaaaga caaagatggt gaagtaagaa ggtctggtat tcatacctat ggcgatacaa
     1561 tccatatttt tatcgagcga aaaaattaca aaggcatttt tcttcccgga tatgaaaagt
     1621 atcaatcgga ctataaccca ccttcaacag gattaaagta tattgatcac atggtaggca
     1681 atgtggagtt aggaggaatg aatgtgtgga gtaaatttta tgctgaagta atgggatttt
     1741 ttaatctcat aacatttgat gacaaagata tttctacaga atacactgct ctgatgagta
     1801 aggtaatgac caatggcaat ggttatatta aatttccgat taacgaacct gccatcggca
     1861 aaaagaaatc gcaggtagaa gagtatcttg atttttatga aggtgcaggc tgtcagcata
     1921 t
//
LOCUS       DAOOOL010000285         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7598_length_1911_cov_82.782866, whole genome shotgun sequence.
ACCESSION   DAOOOL010000285 DAOOOL010000000
VERSION     DAOOOL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7598_length_1911_cov_82.782866"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKU82_12720"
     CDS             complement(<1..391)
                     /locus_tag="PKU82_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOZ83773.1"
                     /translation="MFAVVDIETTGGSLTQGSRITEVAVVIHNGNTIEHRFSTLINPE
                     TDIPSFIIGLTGITNELVENAPCFKEIAEQLFQLLDGKIFVAHNASFDFSFIKSEFKR
                     CGIDFNAEVICTCSLSRKIFPGFGSYSL"
     gene            complement(402..1358)
                     /locus_tag="PKU82_12725"
     CDS             complement(402..1358)
                     /locus_tag="PKU82_12725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOZ83774.1"
                     /translation="MHKILIVQTAFIGDVILATGLIESLHQAFPESKIDFVLRKGNES
                     LLKSHPFVNKLYVWDKKKNKFSNLINLISQIRKEKYDLVVNLQRFFSTGLITLFAKSK
                     NKRGYNKNPLSFCYHKKSEHIIGKKHETYRNFSLIDDLKNIQYQKPNLYFTEEENQKV
                     QSLKSKPFVCMAPASVWFTKQLPVAKWAELTLTIPENIKIYFLGAPADVALCNEIRLL
                     SSHPNIEILCGKLTLTESAALMQHAEMNYVNDSAPLHLASAVNAPVTAFFCSTVPEFG
                     FGPLSDKSIIKQVGEMSCRPCGLHGYKSCPLGHFDCAYKIKI"
     gene            complement(1370..>1911)
                     /locus_tag="PKU82_12730"
     CDS             complement(1370..>1911)
                     /locus_tag="PKU82_12730"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOZ83775.1"
                     /translation="VRVVPHAIARYGSFVAKGVIMMPSFVNIGAYVDSGTMVDTWATV
                     GSCAQIGKNVHLSGGVGIGGVLEPVQASPVIIEDNCFIGSRCIVVEGVRIEQEAVLGA
                     NVVLTMSTKIIDVSGNTPVEYRGVVPARSVVIPGSIPKTFAAGTYQVPCALIIGKRKQ
                     STDNKTSLNDALREYDVAV"
BASE COUNT          589 a          383 c          339 g          600 t
ORIGIN      
        1 ctaaagagta ggagccaaaa ccggggaaga tttttctgct caaagaacaa gtgcagatta
       61 cttctgcatt aaaatcaatt ccgcaacgtt taaattctga ttttataaaa ctgaaatcga
      121 aagacgcatt gtgtgctaca aaaatttttc catcaagcaa ctgaaacagt tgttctgcaa
      181 tttctttaaa gcatggtgca ttttcgacta attcattagt gatgcctgtc agccctatga
      241 taaatgatgg aatgtctgtt tcaggattta tcagtgtaga aaaacgatgt tcaatagtat
      301 tgccattatg aatcactact gcaacttcgg taattcggct accttgtgtt aaacttcctc
      361 ctgtagtttc tatgtcaaca actgcgaaca tgccacgtta cttaaatttt aatcttatat
      421 gcgcaatcaa aatgacctaa ggggcaagat ttatagccat gcaatccaca gggtcggcag
      481 ctcatttcac cgacttgttt aatgatgctt ttgtctgaca aaggaccaaa gccaaactca
      541 ggaactgtag agcagaaaaa tgcagtcaca ggagcattaa cagccgatgc aagatggagt
      601 ggagcagagt cgtttacata gttcatttca gcatgttgca taagtgctgc cgactctgta
      661 agtgttagtt tgccgcataa tatttcaata ttcggatgtg aagaaagcaa ccttatttcg
      721 ttgcaaagtg caacatcagc cggtgcgcca agaaaatata tttttatatt ttcaggaata
      781 gtcagagtta actctgccca cttggctaca ggtaattgtt tggtaaacca aacagaagcc
      841 ggtgccatgc agacaaatgg ttttgatttt aatgactgca ctttttggtt ttcctcctct
      901 gtgaaataca aatttggttt ttgatattga atatttttca agtcatcaat caatgaaaag
      961 ttacgataag tctcatgctt tttaccaatg atatgctctg attttttgtg gtaacaaaag
     1021 gacaatggat ttttattgta gcctcttttg ttttttgatt tagcgaataa agttatcaga
     1081 cctgtgctga aaaatcgttg caggttaaca accaaatcat atttttcttt cctgatttga
     1141 ctgatgaggt taatgagatt agaaaatttg tttttctttt tatcccagac atataattta
     1201 tttacaaaag ggtggctttt caataacgac tcattacctt ttcttaaaac aaaatcaatt
     1261 tttgattctg gaaaagcctg atgcagagat tcaattaatc ctgtagctaa aattacatcg
     1321 ccaataaatg ctgtctgtac aattaatatt ttatgcatac ttgttagtct cagactgcaa
     1381 catcatattc gcgcaatgca tcgttgagtg atgttttatt gtcggtagat tgctttcttt
     1441 tacctataat aagtgcacaa ggcacctgat atgttcctgc tgcaaaagtt ttaggaattg
     1501 aaccgggaat gaccacagaa cgtgcaggca caacaccacg atattcaaca ggtgtatttc
     1561 ctgacacatc aattattttt gttgacatag tcaatacaac attagccccc aaaaccgctt
     1621 cctgttcaat acgaacgcct tcaacaacta tacatctcga ccctataaaa caattgtctt
     1681 caataattac cggtgatgcc tgaactggct ctaaaactcc tccaatgcca actcctccac
     1741 taaggtgtac gttctttcct atctgtgcac aactgccaac agtagcccag gtgtcaacca
     1801 ttgttccgct gtcaacataa gcaccaatat tcacaaacga tggcatcatg atgacacctt
     1861 ttgcaacaaa gcttccatag cgagcaatgg catgtggcac aacacgaaca c
//
LOCUS       DAOOOL010000286         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7714_length_1893_cov_42.753536, whole genome shotgun sequence.
ACCESSION   DAOOOL010000286 DAOOOL010000000
VERSION     DAOOOL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7714_length_1893_cov_42.753536"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKU82_12735"
     CDS             <1..914
                     /locus_tag="PKU82_12735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOZ83776.1"
                     /translation="KERVNLMVDNLISAYKERIKSRTWLEDQTKSAALLKLDKVMRKL
                     AYPDRWRDYSKLSVGKDSYCDNVNRASEFWFQYNLSKLGKPVDKSEWAMSPQTINAYY
                     NPGFNEIVFPAAIMQPPFFDPMADDAVNYGAMGAVIGHELTHGFDDQGCQYDAEGNLK
                     NWWTEKDKSLFDQRAQKLVDQFNHYMPIDSLTINGKLTLGENIADLGGLTIAYAALQK
                     TLALKPKVKIDGFTPEQRFFINFAQVWRNNIRPEELKKRLKTDPHSPGKFRANGTVRN
                     MPEFYEAFGITKENKMYLPPDQRVEIW"
     gene            complement(1113..1655)
                     /locus_tag="PKU82_12740"
     CDS             complement(1113..1655)
                     /locus_tag="PKU82_12740"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOZ83777.1"
                     /translation="MTTFISILRGINVSGHRLIKMDALKELCASLKMQQVKTYIQSGN
                     IVFQSVQGDCDAISKKLEHAILKKFGFAVPVITLSQYEFKSCIEANPFLKDKKLDAAF
                     FHVTFLSEQPSIHDSIETKAVNLKNDLFVFGERCIYLYCPDGYGNTKLTNSFFESKLK
                     VTATTRNWKTVNELLRLAEM"
     gene            complement(1664..>1893)
                     /locus_tag="PKU82_12745"
     CDS             complement(1664..>1893)
                     /locus_tag="PKU82_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOZ83778.1"
                     /translation="GIGTWTNQQFVDRFKMYSDSVYTNLKVAPGTLQTTMPWTMYAGM
                     KQEDLSALFAYLQSLPPVKNTVEKFTVTSGK"
BASE COUNT          601 a          346 c          350 g          596 t
ORIGIN      
        1 caaaagagcg tgtaaacctg atggtggata atttaattag tgcttataaa gaaagaatca
       61 aatcgcgcac ctggctcgaa gatcaaacca agtcggcagc tttattaaaa cttgataaag
      121 taatgcgcaa attggcttac cctgatagat ggcgcgacta cagtaaatta tcagtaggca
      181 aagattcata ctgcgacaat gttaaccgcg cttctgaatt ttggtttcaa tataacttat
      241 ccaaattagg aaagcctgtt gacaagtcag agtgggcaat gtcgccacaa accatcaatg
      301 catattacaa tcccggattt aatgaaattg tttttcctgc tgcaataatg caaccaccat
      361 tctttgatcc tatggctgac gatgccgtaa actatggtgc tatgggtgcc gttatcggac
      421 atgagttaac tcatggtttt gatgatcagg gttgtcagta tgatgctgaa ggtaacttaa
      481 aaaactggtg gacagaaaaa gacaaatctt tattcgatca gagagcacaa aagttagtgg
      541 atcagttcaa ccattatatg cctattgact cactcaccat taatggcaaa cttactcttg
      601 gtgaaaacat tgccgattta ggcggactaa ctattgccta tgctgcatta cagaaaacgc
      661 ttgccttaaa gcctaaagta aaaattgacg gctttactcc ggaacaacgt ttctttatca
      721 actttgctca ggtatggcgc aataacattc gccccgaaga attaaaaaag agattaaaaa
      781 cagatccgca cagcccggga aaattccgtg ctaatggcac tgtacgtaat atgcctgagt
      841 tttatgaagc atttggtatt acaaaagaga ataaaatgta tttacctccc gaccaaagag
      901 tagaaatctg gtaatattta ttacagaaat ttcagaaggc tgcattcaac aagtgcagcc
      961 ttttttgttt aaatatttta tatgtgttca aacattgtgg tcggcaataa aattttacga
     1021 caaatttatt gatgtttatt gctccaatag gcttgtggta tattcaatca agagtgaaaa
     1081 gaattgatga agcttctaaa aataaatctg atttacatct cagccaaccg caacagctca
     1141 tttacagttt tccagtttct ggttgtagct gtaactttaa gtttgctttc aaaaaaacta
     1201 tttgtcagct tagtgtttcc atatccatcg gggcagtaga ggtaaatgca cctttcacca
     1261 aaaacgaaaa ggtcgttttt tagattgaca gcttttgttt ctatcgaatc atgaatgctt
     1321 ggttgttctg aaagaaaggt gacatgaaaa aatgccgcat ccaatttttt atcttttaaa
     1381 aaaggatttg cctctatgca acttttaaat tcatactgcg acaatgtgat gacaggtact
     1441 gcaaatccaa atttttttaa aatagcgtgt tctaactttt tgcttatagc gtcacagtca
     1501 ccttgtacag attgaaaaac aatgttgccg ctttgaatgt aggttttcac ttgttgcatt
     1561 tttaaagaag cacaaagttc ttttaatgca tccattttta tcaaccgatg tccactgaca
     1621 tttatgcctc ttagaataga aataaaagtg gtcattaaaa ttgttactta ccgcttgtaa
     1681 ctgtaaattt ttcaacagta ttttttaccg gtggtagact ttgcaaataa gcaaacaaag
     1741 ctgaaaggtc ttcttgcttc atgcctgcat acattgtcca tggcattgtt gtttgcaatg
     1801 tgccgggtgc tactttcaaa ttagtgtaaa cgctgtcaga atacatttta aatcggtcaa
     1861 caaactgctg atttgtccaa gttcctatac ctg
//
LOCUS       DAOOOL010000287         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_7824_length_1874_cov_59.999450, whole genome shotgun sequence.
ACCESSION   DAOOOL010000287 DAOOOL010000000
VERSION     DAOOOL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7824_length_1874_cov_59.999450"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKU82_12750"
     CDS             <1..593
                     /locus_tag="PKU82_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83779.1"
                     /translation="LIVPKNSTHLLFPKLARWIPSAMFEWLSGIRNTKGIVAVFLIVL
                     YATCFLKFIPLIFLWLTTITIAGFYQFGEPLSLLYLHENAKQLLRIKIKNVLLLYVKI
                     TLIPVLVNIFLHPDIWWVDLMIYILCMLVIICAVLIKYAVYRPNERLTAGSMLLSIVS
                     ISPALPFTLPLPIIVAAIKYKSAIQNLENYFHDQHK"
     gene            577..1257
                     /locus_tag="PKU82_12755"
     CDS             577..1257
                     /locus_tag="PKU82_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ83780.1"
                     /translation="MISINNLKISFDKHQVLDIEKYNFNTGKVHGIIGLNGAGKTTFF
                     NAVCKFLKRDEQAVLLNNKPFERHDAAYLETTNYFYDNITAEEYLSLFPSDNLLFNKK
                     NLIELLQLKSGELIETFSTGMKKKLALIAIICQDKPVYILDEPFNGLDLESNKILEML
                     IQKLVQNGKTIFLSSHILSPLTTSCAEIHLLQNGRFEKQFQPEQFNEIENELFSNLTE
                     KVNSILKK"
     gene            complement(1259..>1874)
                     /locus_tag="PKU82_12760"
     CDS             complement(1259..>1874)
                     /locus_tag="PKU82_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOZ83781.1"
                     /translation="QGAEHSSARMERFLTLSAEYNMVVTNCTTPANFFHALRRQLKWP
                     FRKPLIVFTPKSLLRHPLCVSTISDFADKKFSEVIDDTVKATEVSRVILCTGKVYYDL
                     LQHRTAEKITDVALIRLEQLYPFPQQQVEKLLKKYSKADDIIWLQEEPDNMGAWGFIK
                     RMMPDLNIRLIARVESASPATGSHSAHDEEQQQLLKAAFEKVTV"
BASE COUNT          598 a          337 c          313 g          626 t
ORIGIN      
        1 ctttaatagt accaaaaaat tcaacacatt tactttttcc aaaacttgca aggtggatac
       61 catcagcaat gtttgaatgg ttgagtggca tacgcaacac taaaggcatt gtcgcagtct
      121 ttttaattgt actgtatgcg acctgtttct taaaatttat tccattgata tttctttggc
      181 tgacaacaat aacaattgcc gggttttatc aatttggtga accactttca ctactctatt
      241 tacatgaaaa cgccaaacag cttttaagaa ttaaaataaa aaatgttttg ttgctttatg
      301 taaagataac tctcattcct gttttagtga atatatttct ccatccggat atttggtggg
      361 ttgacttaat gatttatata ctttgtatgc ttgttattat ttgtgctgta ctgattaaat
      421 atgctgttta caggcccaat gaaagattaa ctgcaggaag catgttactg tcaattgttt
      481 caatttcacc ggcacttcca ttcacacttc cattacctat tattgttgct gcaataaagt
      541 ataaaagcgc tattcaaaac ttagaaaatt actttcatga tcagcataaa taatctgaaa
      601 atttcctttg ataaacatca ggttcttgat attgaaaaat ataattttaa tacaggaaaa
      661 gtacatggca ttataggtct taatggtgcc ggaaaaacaa cattttttaa tgctgtatgt
      721 aaatttttaa agcgtgatga gcaagccgtt ttgttaaata ataaaccttt tgagcgtcat
      781 gatgcggcct atctggaaac aacaaattac ttttatgata acattactgc cgaagaatat
      841 ctctcgcttt ttccatcaga taacttactt ttcaataaaa agaatttgat tgaactgctt
      901 caattaaaat ccggtgagtt gattgaaact ttctcaacag gcatgaaaaa gaaactggct
      961 ttgattgcaa taatctgtca ggataaaccg gtgtatattt tagatgagcc ttttaatgga
     1021 ttggatttag agtcgaataa aattctggaa atgctgatac agaaacttgt acaaaacggc
     1081 aaaacaattt ttctctcatc acacattctt tctccattaa caacatcatg tgctgaaata
     1141 catttacttc aaaacggacg ttttgaaaaa caatttcaac cggaacagtt taatgaaata
     1201 gaaaatgagt tgttttccaa tctcaccgag aaagtcaatt cgattttgaa aaaataattt
     1261 atacagtaac tttttcgaat gctgctttaa gcaactgttg ttgctcttca tcatgagcac
     1321 tgtgcgatcc ggttgcaggg ctggcacttt caacacgtgc aatgagtctg atgtttaagt
     1381 caggcatcat tcgttttata aaaccccatg ctcccatatt atcaggctct tcctgcagcc
     1441 aaataatgtc atcagccttt gaatatttct ttaaaagctt ttcaacctgc tgctgcggga
     1501 aaggataaag ttgctcaagt ctgattagcg ctacatcagt aatcttttct gctgtgcgat
     1561 gttgtaataa gtcgtaatat acttttcctg tacacaaaat tactcgcgat acttcggtag
     1621 ctttaacagt atcatcaatc acctcactga attttttatc agcaaaatca ctgatagtag
     1681 aaacacaaag cggatgacga agtaaactct ttggtgtaaa cacaatcaaa ggttttctaa
     1741 aaggccactt tagctgacgt cttaaagcat ggaaaaaatt tgcaggagta gtacagttgg
     1801 tcacaaccat attgtattct gcacttagtg taagaaagcg ttccatacgt gcgctgctgt
     1861 gttcggcacc ttgc
//
LOCUS       DAOOOL010000288         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_8443_length_1783_cov_21.905093, whole genome shotgun sequence.
ACCESSION   DAOOOL010000288 DAOOOL010000000
VERSION     DAOOOL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8443_length_1783_cov_21.905093"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1274)
                     /locus_tag="PKU82_12765"
     CDS             complement(102..1274)
                     /locus_tag="PKU82_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOZ83782.1"
                     /translation="MGFSAGVKAQDGKALFDANCKSCHAMNDVVIGPALKDVHKKFSA
                     EWLHKWIKNSQALIKAGDKDAVAVFNEYNKMVMPNQNLSDAEIDAVLAYIGKESEQAP
                     VAAAPTAPAPGTVAPEKDNSTLYWVIAIILILLALAATLNKIRKGLEYAVREKEGIPH
                     PPHLEPKEARSLWISKNKKLIAVVLLLLVVLGSVKGWYALKDIGVYTNYEPEQPIHFS
                     HKLHAGDNKIACLYCHSGADKSRHANIPSASTCMNCHKYVKEGPVYGDKEIKKIYAAL
                     DWDGSKFGTNQKPIQWIRVHSLPALAYFNHAQHVTVGKIECQTCHGPVQEMDVVKQFS
                     PLTMGWCINCHRETEVKMDGNGYYDEVHKTLAAKYGKDAKLTVDKIGGLDCARCHY"
     gene            1568..>1783
                     /locus_tag="PKU82_12770"
     CDS             1568..>1783
                     /locus_tag="PKU82_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83783.1"
                     /translation="MIKIVKSAFFIICCILIHLQIKAQTSDDSLTHVLIKKHIAYNLA
                     KPYSQGYRIQLYFGSQRDKAYEFRTEFI"
BASE COUNT          514 a          357 c          292 g          620 t
ORIGIN      
        1 cttctacacc tttccaatat cgtttttcca tatattattt aaaaagtttg tggccgaatg
       61 tttttaattt gaatgaacag attcagcttt tagtgttttg attaatagtg acagcgggca
      121 caatcaagac cgccaatttt gtcaactgta agtttagcat ctttaccgta cttggctgct
      181 aatgttttat gcacttcgtc ataatatcca ttaccatcca ttttcacctc tgtttcgcgg
      241 tggcaattaa tacaccaacc catagttaat ggtgagaatt gtttcaccac atccatctcc
      301 tgaacaggac cgtgacaagt ctgacattcg atttttccaa cagtaacgtg ctgagcatga
      361 ttaaaatacg ccaatgcagg taaactatgt actctgatcc attgtattgg tttttgattg
      421 gttccaaatt tagaaccatc ccaatcgagg gctgcataaa ttttcttgat ttctttatcg
      481 ccatacacag gaccctcttt cacatatttg tgacaattca tacaggtact tgctgatgga
      541 atattggcat gacggctttt gtctgcacca ctatgacagt atagacaggc tattttatta
      601 tctcctgcat gcagtttatg tgaaaaatga ataggttgct caggctcata attggtatat
      661 acaccaatat cctttaaagc ataccaacct ttaacactac ctaagacaac taataaaagt
      721 aatacaactg caatcagttt tttattctta ctaatccata aactacgtgc ttcttttggc
      781 tctaagtgtg gggggtgtgg aatgccttct ttttcgcgta ctgcatactc aagacccttt
      841 ctaattttgt taagtgttgc tgcaagggct aacagaatca ggataatagc tattacccaa
      901 tataaagtag agttatcttt ttcaggagca actgttccgg gtgcaggtgc tgttggtgca
      961 gcagctacag gtgcctgctc actttcttta ccaatgtatg ccaatactgc atctatttca
     1021 gcatcagaaa gattctgatt aggcataacc attttattat actcattgaa aactgctacg
     1081 gcatccttat ctccagcttt tattaatgct tgcgagttct ttatccattt atgtagccat
     1141 tctgcactga attttttatg tacatcctta agtgccggac caatgacaac atcattcatt
     1201 gcgtggcatg atttacagtt ggcatcaaaa agtgctttac catcctgtgc tttaactcct
     1261 gctgaaaatc ccaatatcag gagcatgaaa aacaacccct tacacttgac aagaaggtta
     1321 ttagtgagct tttggtgcat aaattgtttt tgttttcgta tcaatttata atttagttca
     1381 aactgcaata attccgtaaa attccagttt ggagcgcgca aatttataac ttaccttatt
     1441 taattcataa aattttcaaa ttaaaattta gtctaaataa cagcctttta ggtttttttt
     1501 tactatgccc ctcaatttta ggctgttttt gcagcccgtt ttacatataa tctgtaatat
     1561 tgaagccatg attaaaattg taaagagtgc cttctttatt atatgttgca ttctaattca
     1621 tttacaaatc aaggctcaga ccagtgatga tagtctgact catgtactta taaaaaagca
     1681 tattgcctat aatctggcta aaccctattc acaaggctac cgcattcaac tttattttgg
     1741 gtcgcaacgt gacaaagcct atgagtttag gacagaattt ata
//
LOCUS       DAOOOL010000289         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_8507_length_1775_cov_21.868023, whole genome shotgun sequence.
ACCESSION   DAOOOL010000289 DAOOOL010000000
VERSION     DAOOOL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_1775_cov_21.868023"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..996
                     /gene="fabD"
                     /locus_tag="PKU82_12775"
     CDS             112..996
                     /gene="fabD"
                     /locus_tag="PKU82_12775"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOZ83784.1"
                     /translation="MSSKRAYIFPGQGSQFSGMGKDLFEQSDAARSIFEKANSITGFS
                     ITDVMFSGTDEELKQTKVTQPAIFVHSCAIIASLGDNFHPDMVAGHSLGEFSALVANK
                     CLSFEDGLKLVMERALAMQAACELKPSTMAAILGLDDEKVESICAGINDVVVCANYNC
                     PGQLVISGTIDGVKTACEQLTAAGARRALLLPVGGAFHSPLMEPARERLANAIANTTF
                     AVPICPVYQNVNALPSTDPQQIKQNLIAQLTAPVRWTQTVIKMANDGATDFIEAGPGK
                     VLQGLVKKIHKEAQVMSA"
     gene            1023..>1775
                     /locus_tag="PKU82_12780"
     CDS             1023..>1775
                     /locus_tag="PKU82_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOZ83785.1"
                     /translation="MEKATQYSEQNKDRFLNELLELLRIPSVSADSKYKADLVRTAEF
                     VKSSLEKAGADKVEICPTGGHPIVYGEKIIDPKLPTVLVYGHYDVQPPDPLDLWHSPP
                     FEPVIKKTDLHPEGAIFARGSCDDKGQMYMHVKAFESMMKTATLPCNVKFMIEGEEEV
                     GSSNLGIFVKANKERLKADVVLISDTSMIANDTPSIDVGLRGLAYMEVEVTGPNRDLH
                     SGVYGGAVANPATILCQMIASMHDENNHITVKG"
BASE COUNT          564 a          321 c          372 g          518 t
ORIGIN      
        1 gtttacacca tattttcaga attaggcagc ttgttttatt ttgttttact tagttttggc
       61 aaaagatttt cacctttaat caaaacatct gaattaactg actaatattt tatgagttca
      121 aaacgtgcat atatttttcc cggacaaggt tcacaatttt cgggaatggg aaaagatttg
      181 tttgagcagt ctgatgctgc tcgcagtatt tttgaaaaag caaacagcat taccggcttt
      241 tcaattactg atgtaatgtt ttcaggaacc gatgaagaac tgaaacaaac aaaagttaca
      301 cagcctgcaa tttttgttca ttcatgtgca atcatagcat cgcttggcga caattttcat
      361 cccgacatgg ttgcaggaca ttcattagga gaattttctg cattggtagc caacaagtgt
      421 ttgagttttg aagatggatt aaaattggtg atggaacgtg ctcttgccat gcaggcagca
      481 tgcgaattga aaccttcaac tatggcagca attcttggcc ttgatgacga aaaggtagaa
      541 tcaatttgtg caggaataaa tgatgttgtt gtttgtgcta actataattg cccgggacaa
      601 ttagtcattt ccggaactat tgacggagta aaaactgcat gtgaacagct tactgctgcc
      661 ggtgcaagac gtgcactact gcttccggta ggaggagcgt ttcattcacc tctcatggag
      721 cctgcacgcg aaagattagc caatgcaatt gctaatacaa catttgcagt accaatttgc
      781 cctgtatatc agaatgtaaa tgcattgcct tcaaccgacc cgcaacaaat caaacaaaat
      841 cttattgccc aacttactgc tcctgtacga tggacacaga cagttattaa aatggcaaat
      901 gatggcgcta ctgattttat agaagccgga cccggaaaag ttttgcaggg actcgtaaag
      961 aaaattcata aagaagctca ggtaatgagt gcataattta taatcattaa taaaaagttt
     1021 aaatggaaaa agcaacacaa tattcagaac agaataaaga ccgtttttta aacgaactgc
     1081 tcgaattgct gcgcatacct tcagtaagtg cagactcaaa gtataaagca gatttggtaa
     1141 gaacagcaga gttcgtaaaa tcaagtcttg aaaaagcagg tgccgataaa gtagagattt
     1201 gtcctacagg cgggcatcct attgtttatg gcgaaaaaat tattgatcca aaactcccaa
     1261 ctgttttagt ttatggacat tatgatgttc agccacccga tccattggat ttatggcatt
     1321 caccaccgtt tgaaccggta attaaaaaga cagatttgca tcccgaagga gccatctttg
     1381 cacgtggatc ttgcgatgac aagggtcaga tgtacatgca tgtaaaagca tttgaaagta
     1441 tgatgaaaac tgctacgctt ccatgcaatg ttaagtttat gattgaaggt gaagaagaag
     1501 ttggttcatc aaaccttggc atatttgtta aagcaaataa agaacgtcta aaggctgatg
     1561 ttgtattgat ttccgacact tcaatgattg caaacgatac accatctatt gatgttggct
     1621 tgcgcggatt ggcatatatg gaagttgaag ttacaggacc taaccgcgat ctgcatagtg
     1681 gtgtttatgg tggtgcagtt gcaaatcctg caacgatact ttgtcagatg attgcttcaa
     1741 tgcatgatga aaacaatcat atcacggtta aagga
//
LOCUS       DAOOOL010000290         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_8541_length_1769_cov_158.476079, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000290 DAOOOL010000000
VERSION     DAOOOL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_1769_cov_158.476079"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKU82_12785"
     CDS             <1..217
                     /locus_tag="PKU82_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83786.1"
                     /translation="NEEHSIYYNKVTFNNLQQSDEKMFMHTANMNPEERMAYLQKLRE
                     TTQENISEAEKRAILNQIKINPPDENT"
     gene            282..1241
                     /gene="cas1"
                     /locus_tag="PKU82_12790"
     CDS             282..1241
                     /gene="cas1"
                     /locus_tag="PKU82_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOZ83787.1"
                     /translation="MIKRTLYFGNPAYLSLKNSQLMVRLPEVEKNQQLDVAFKQQAHA
                     TIPVEDIGIVLLDHQQITITHGLLDALMENNVAVITCNNTRHPAGLLLPLQGHTLQHE
                     RYSDQLSASEPLRKQLWQQTIIQKIKNQAAGLQLQNIDSTRLYPIYNNVKSGDSENAE
                     GVAAAFYWSNIFTKIPATIEHAPGNLFKRQRDGLPPNNYLNYGYAILRATMARSIVAA
                     GLLPTKGIHHHNRYNAYCLADDLMEPFRPFVDRIVIDIILKHGIIDDLTKAIKADLLT
                     IPVIDVMMDGENSPLTIATQRTATSLVKCFAGEQRKLIYPELL"
     gene            1238..1576
                     /gene="cas2"
                     /locus_tag="PKU82_12795"
     CDS             1238..1576
                     /gene="cas2"
                     /locus_tag="PKU82_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOZ83788.1"
                     /translation="MNFERLNAYRTMWTIVMYDLPTETKKQRKLAAKFRKDLLQDGFQ
                     MFQFSMYLRHSSSSENADVHRNRVKKSLPTEGKVGILQITDKQFGQMEIFYGKKAHDK
                     PQTSQQLELF"
BASE COUNT          626 a          336 c          328 g          479 t
ORIGIN      
        1 aaatgaagaa cattctattt actacaataa agttacattc aacaaccttc aacaatccga
       61 tgaaaaaatg tttatgcata cagccaacat gaatcctgaa gagcgtatgg cgtatttgca
      121 aaaactgcgt gagacaacac aagaaaatat ttctgaagca gaaaagagag ccattttaaa
      181 tcagataaaa ataaatccgc cagatgaaaa tacttgattt ggaacaattg caacaaattc
      241 atcgtttaaa aaacaagaaa tcgtgatgag ataaaaaaca aatgattaaa cgtacgctct
      301 attttggcaa ccctgcatac ctgagcttaa agaacagtca gctaatggtg cgtttgcccg
      361 aggtggagaa aaaccaacaa cttgatgttg cgtttaaaca acaggcacat gctaccattc
      421 ctgttgaaga tattggtatt gtattgctcg accatcaaca aattaccata acgcatggat
      481 tactcgatgc attaatggag aacaatgttg ccgttataac atgcaacaat acccggcatc
      541 ctgcaggact gttgctgcca ctgcaagggc acactttaca gcacgaacga tacagtgatc
      601 agctttcggc cagcgagcct ttacggaaac aactttggca gcaaaccatt attcaaaaaa
      661 ttaaaaatca ggcagcagga ttacaattac aaaatattga ttccacacgc ctctacccta
      721 tctacaacaa tgtaaaaagt ggcgatagtg agaatgccga aggtgttgca gcagccttct
      781 actggtcaaa tatttttaca aaaattcctg ctacaataga acatgcaccg ggtaatttat
      841 tcaaacgaca acgtgatggt ttgccaccca acaattatct gaattatggt tatgcaattc
      901 ttagagcaac tatggcaaga agtattgttg ctgcagggct cttaccaaca aaaggtattc
      961 atcatcataa ccgttacaat gcctactgcc ttgcagatga cctgatggag cccttcagac
     1021 cttttgttga cagaattgtt attgacatta tcttaaaaca tggtataatt gatgacttga
     1081 caaaagctat taaagcagat ttattgacaa ttcctgtaat agatgtaatg atggatggag
     1141 aaaacagtcc attaacaatt gctacacagc gaacagctac ttcattagta aaatgttttg
     1201 ccggtgaaca acgcaaacta atttacccgg agttgttatg aattttgaac gcctcaatgc
     1261 ctatcgcact atgtggacta ttgttatgta cgacctgcct actgaaacta aaaagcaaag
     1321 aaaattagct gctaaattca gaaaagattt attgcaggat gggtttcaaa tgttccaatt
     1381 cagtatgtac ttgcggcata gcagcagtag cgaaaatgct gatgtgcatc gaaacagagt
     1441 aaaaaaatca ttgcctactg aaggtaaggt tggaattttg caaattacgg ataaacaatt
     1501 cggtcagatg gaaatttttt atggcaaaaa ggcgcatgac aaaccacaaa catcgcaaca
     1561 acttgagcta ttttaaaggg tgatttaagt caatccgtta aatttcagga ataaaaaatc
     1621 ccgctgtttg ggcgggactt caagttctta aactcatttt tactatcctg aaactgttct
     1681 tttaagttga atatctgttg tttagccaat agatattgaa agaactctga caaagataaa
     1741 agttgaaagc aaatcacaac gcggctttg
//
LOCUS       DAOOOL010000291         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_8836_length_1729_cov_95.168459, whole genome shotgun sequence.
ACCESSION   DAOOOL010000291 DAOOOL010000000
VERSION     DAOOOL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8836_length_1729_cov_95.168459"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKU82_12800"
     CDS             complement(<1..1013)
                     /locus_tag="PKU82_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83789.1"
                     /translation="MKQIYTTMIALASAFCAHAQITLTQSDFAGAGDNILVSNADATI
                     PIDFTTTGANQTWDFSSMIAQSQDTLEFLSVASTGGTYSVYFANVGFNSNRSNIAVAA
                     GTLPTVPGLPITLSDPYSFYYKGSGDYKQQGLGITISGFQTPIAYSSKDILYNFPIAF
                     NDVDSCQAAWNFSLSGLGYYGFEQKRVNHVDGWGTVITPYGSFNALRIKTELYAHDTL
                     YADTLGFGYSMDRPKAVEYKWIANNEKIPVLQINTTDVLGTETVASVVYIDSLRSVGI
                     SSTELLSNFSVAPNPAEKYFVISYSLTKPGQVSIKLTDIKGVEVMNEMQGIQSTGSHQ
                     KMIG"
     gene            complement(1034..>1729)
                     /locus_tag="PKU82_12805"
     CDS             complement(1034..>1729)
                     /locus_tag="PKU82_12805"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOZ83790.1"
                     /translation="FQLHFNTENNRLLVVDSFSAIQTPRRYRNEYGQFEEHSPFCERD
                     FRRPQNLKTFDEKGEFLVMIKKESIVYPFVYATHPFDAIGWDGYCYPYIFSIHDFEPI
                     TGRIHLPPPIHQTFEAHNFVVCSFVPRLFDYHPLSIPVPYNHSNIDSDEMLYYVDGDF
                     MSRKNVTKGQITLHPAGIPHGPHPGTVEKSLGAKETKELAVMIDTFKPLWLTKEAMNI
                     IIPDYYKSWVENG"
BASE COUNT          555 a          327 c          301 g          546 t
ORIGIN      
        1 cctatcattt tctgatggct tcctgtgctt tgaatacctt gcatttcatt cataacttca
       61 acgcctttta tatcggtaag tttaatggaa acttgtccgg gtttagtcag agaataacta
      121 attacaaaat atttctcggc aggatttggt gcaacagaaa aattactcaa caattcagta
      181 gaagatatac ctacgcttcg caatgaatca atatacacaa cagatgcaac agtttctgtt
      241 cctaaaacat cagtagtgtt tatctgtaat accggaattt tttcattgtt ggcaatccat
      301 ttgtactcta cagccttagg cctgtccatt gaataaccaa aacctaatgt atcggcatat
      361 aatgtatcat gtgcatacaa ttctgtttta atacgtaggg cattgaaact tccataagga
      421 gtaataactg ttccccaacc atcaacatga ttgacacgtt tttgttcaaa accataatat
      481 cctaatccac ttaaagaaaa attccaagct gcctgacagg agtcaacatc attaaaagca
      541 atcggaaaat tatacaatat atctttactg ctataagcaa ttggtgtctg aaatccgcta
      601 atagttatgc ctaagccttg ttgtttatag tcaccgctac ctttgtaata aaaactgtat
      661 ggatcactca atgtaattgg caatccggga accgttggta atgtacctgc agccactgca
      721 atattagaac ggttactgtt aaaaccaaca ttagcaaaat atacagagta tgttcctcct
      781 gttgaagcta cacttaaaaa ctcaagggtg tcctgacttt gtgcaatcat tgaactaaaa
      841 tcccatgttt gatttgctcc tgttgtagta aaatcaatcg gtatggttgc atcagcattg
      901 ctgacaagaa tgttatcacc tgcaccggca aaatcacttt gcgtgagggt aatttgagca
      961 tgggcgcaaa aagccgatgc taaggcaatc atggttgtgt aaatttgttt catttatatg
     1021 cttgtttaaa ttgttatccg ttttctaccc atgatttata ataatcagga ataataatat
     1081 tcattgcttc ttttgtaagc caaagaggtt taaaagtatc tatcatcaca gccaactctt
     1141 tagtttcttt agcaccaagt gatttttcaa ctgttccggg atgtggtccg tggggaatac
     1201 cagcaggatg taatgtaatt tgtccttttg taacattctt tctgctcata aaatcgccat
     1261 caacatagta tagcatttca tcagaatcaa tattggaatg attataagga acaggaattg
     1321 aaagcggatg atagtcaaac aaacgaggta caaatgagca aacaacaaaa ttgtgtgcct
     1381 caaaagtctg atgtataggc ggtggtaaat gtattcttcc tgttataggt tcaaaatcat
     1441 gaatagaaaa aatgtaaggg tagcagtatc catcccatcc aatagcatca aacggatgtg
     1501 tagcatatac aaaaggataa acaatgctct cctttttaat cataacgaga aactctccct
     1561 tttcatcaaa ggtcttcaga ttttgtggtc tgcgaaaatc gcgctcgcaa aaaggactgt
     1621 gttcttcaaa ttgtccatac tcatttctat atctgcgtgg agtttgaata gcactaaaag
     1681 aatccactac cagtaatctg ttgttctctg tattaaaatg cagttgaaa
//
LOCUS       DAOOOL010000292         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_8914_length_1717_cov_52.169073, whole genome shotgun sequence.
ACCESSION   DAOOOL010000292 DAOOOL010000000
VERSION     DAOOOL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8914_length_1717_cov_52.169073"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="PKU82_12810"
     CDS             complement(<1..1672)
                     /locus_tag="PKU82_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOZ83791.1"
                     /translation="MYSTRKKVNQGLLQHIPLVGTLVSKSKEWITSVKLEMFYSKDEI
                     LEMYFNTVDFGNNWFGIKVAAQNYFSKQPSELNLQESATLIGLLKATSSYNPLNNKKR
                     SLARRNIVLSQMQKYGYISQAAFDSISPMPLNLKRPTNNPATKDSYLRQLIAKIIKSY
                     CDENNLNINEDGLKIYTTINSHLQNYAELAVKDHLRKVQKQFDEHWGKQNPWRDDNGR
                     EILNFANTAVAFTQSYDRLKKQFPSAPDSVNFYLNKKKSMKVFTYNGAKDTVMSTLDS
                     LKYYARLLNAGTMSLNPLTGEIKAYVGGVDFNFFKFDHVTQSKRQPGSTFKPFAYLAA
                     IEDTFSPCSRLDDVPVSIEYDGGQIWQPYNSTGAFTYSSKTLRRALAQSVNSITAQIA
                     ELVGWDKIVECAHRAGVKSRLDTVPSICLGTSDVSVYEMTCAYAPFVNGGKSVEPILV
                     KKICANDGTVLKEFMPEFKQVISEENAWLMLYMLQGTIQEPGGTSQALWGYSVFKNGN
                     DVAGKTGTTSNYSDAWYMGVTHDLVTGVWVGAPYRSVHFRNSSGQGSRL"
BASE COUNT          509 a          364 c          276 g          568 t
ORIGIN      
        1 ctaatcgtga cccctgacct gatgaatttc gaaaatgcac gcttctatat ggagcaccta
       61 cccaaacacc ggtaaccaaa tcatgagtaa cacccatata ccaagcatcg gaataattgg
      121 atgtagttcc tgttttgcct gcaacatcat ttccattttt aaaaactgaa tagccccaca
      181 atgcctgaga tgttccacca ggctcctgaa tagttccttg caacatatac aacattaacc
      241 aggcattttc ttcagagata acttgcttaa attctggcat aaactctttg agaactgttc
      301 catcgttggc acaaattttc ttcaccaaaa tgggttcaac actttttccg ccatttacaa
      361 atggtgcata agcacaagtc atttcgtaaa cacttacatc acttgttcct aaacaaattg
      421 atggcacagt atctaaacga ctttttacac ctgccctatg tgcacactca acaattttgt
      481 cccatccaac caattcagca atttgtgctg taatagaatt gaccgattgt gccaacgccc
      541 gtcttaatgt tttacttgaa taagtaaatg cacctgtact attatagggt tgccagattt
      601 gaccaccatc atactctatg gatacaggca catcatctaa tcggctacaa gggctaaaag
      661 tatcttctat tgctgccaaa taagcaaacg gcttaaatgt tgaacccggc tgtcgttttg
      721 attgtgtaac atgatcgaac ttaaaaaaat taaaatcaac accacctaca taagctttaa
      781 tctcaccggt taacggattc aaagacatag ttcctgcatt taacaaacgt gcataatatt
      841 taagtgaatc taatgttgac atcacggtat ccttggcacc attgtatgta aatactttca
      901 tgcttttttt cttgttaaga taaaaattaa cagaatctgg tgctgaaggg aattgctttt
      961 tcaaacggtc gtatgactgc gtaaatgcca cagcggtatt tgcgaagttc aatatttctc
     1021 ttccattgtc atcgcgccat ggattttgtt ttccccaatg ttcatcgaat tgcttctgca
     1081 cttttctcag atgatcttta acagccaact ctgcataatt ttgtaggtgt gagttaatgg
     1141 ttgtataaat tttcaatcca tcctcattaa tgtttaagtt gttttcatca caataacttt
     1201 taataatctt tgctattaac tgcctcaggt aagaatcttt tgttgcaggg ttattggttg
     1261 gacgtttaag gttcaatggc attggactga tggaatcaaa agctgcctga ctgatatacc
     1321 catacttctg catctgcgac aacacaatat ttcttcttgc caatgagcgt ttcttattat
     1381 tcaaaggatt ataactactt gtagctttga gcaaaccaat gagtgttgct gattcctgca
     1441 aatttagttc tgaaggttgt ttgctgaaat agttttgtgc agcaaccttg ataccaaacc
     1501 aattattacc aaaatcaact gtattaaaat acatttccaa tatctcatcc tttgaataaa
     1561 acatctctaa tttaacagat gttatccact cttttgattt gctaaccaat gtaccaacaa
     1621 gaggaatatg ttgaagtaat ccctgattaa ctttcttgcg ggtactgtac atatttttgg
     1681 ctagctgttg tgttattgtg ctagcaccac gctcatc
//
LOCUS       DAOOOL010000293         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9162_length_1686_cov_70.576334, whole genome shotgun sequence.
ACCESSION   DAOOOL010000293 DAOOOL010000000
VERSION     DAOOOL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9162_length_1686_cov_70.576334"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..1108)
                     /locus_tag="PKU82_12815"
     CDS             complement(368..1108)
                     /locus_tag="PKU82_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83792.1"
                     /translation="MEEKYIKWATITIFVCGGLILIANYAKDYFTKEKARKEKEAAQR
                     ENAMQGEIVAPESKPAPHSQAAQLPEEMVELKIGGMTCAFKKSQFEAGVETPKCLQVG
                     NTVPVIIKLKGGKLFISTKIFDKAGKMVCEIDDNEWTVNKNNFYKRNYDSTAVEVIDQ
                     YDNIVLSAEVKNKNSIHVYGIFNAIGQAFIFAYGDVTETLEYFPQAIKIIEQMEDRPW
                     DELYREKAKKIPRVFLYNGKDYLGKRVS"
BASE COUNT          534 a          299 c          281 g          572 t
ORIGIN      
        1 cagatgaata aaaaacttgg tggcatagaa acagtatttg ttatatcaag tcctgaaaat
       61 gcaccacttt catcaaccat attgcgcgac attattatca atggcggcag tgtggaaggt
      121 ttggttcctg tgaagattga aaatcagaaa aaataaatta tttttttagt aggcactatt
      181 gcctctgcaa gtcaaaacaa ggttttctat attattttag atgcataagc ggtttgacaa
      241 atacacttag cgaactttga atgcgactgg aaattgtaag agttaaaggg cgcgagcgga
      301 aatgatgctt gcggctataa agggggaatt catgtctttt gagtttagta aatctgttta
      361 attgatgtta cgaaacacgt ttgcctaaat aatctttacc attgtataag aacacacgtg
      421 gaattttctt tgctttctca cgatatagct catcccaagg tctatcttcc atttgttcaa
      481 taattttaat tgcttgagga aaatactcaa gagtttcggt tacatcacca taagcgaaaa
      541 tgaacgcttg tccgattgca ttaaaaatac catacacatg aatgctgttt ttgtttttta
      601 cttctgccga taaaacaatg ttatcatatt ggtcaatcac ttcaactgct gttgaatcat
      661 aatttctttt gtagaaatta ttcttgttaa ccgtccattc attgtcatca atttcacaaa
      721 ccattttgcc ggccttatca aatattttag ttgaaataaa aagtttacca cctttcaatt
      781 tgataataac tggaacagta tttccaactt gcaaacattt tggagtttca acacccgctt
      841 caaattgact tttcttaaat gcacatgtca tacccccaat tttcaattca accatttctt
      901 caggtagttg tgctgcttgt gaatgcggtg ctggtttgct ttctggtgct acaatttcac
      961 cttgcattgc gttttcacgc tgcgctgctt ctttctcctt tcgggctttc tctttcgtaa
     1021 aataatcttt ggcataattt gcaattaaaa ttaacccacc acaaacaaaa attgtaattg
     1081 tcgcccactt aatatatttt tcttccattg tcgttatgaa ttagcactaa agtaaagttt
     1141 attagaattc caaaagttga aagttagcag gatgccattt gcgcataaca cagcctttcc
     1201 gataactatc ggaattaaaa ctaatcagta gctcaaaagt aaaaagaaaa atgattcaag
     1261 gaggcaatag caaagaatca ggttgtatta aaattaattg tttgtgttaa atcacgctgt
     1321 caggggcggt gcgacctgac ccttaggttt gtgaaatcaa aaaaatgtaa atgcacatta
     1381 tatcattaat aattatttgg tatttttatc cgtgatttaa taacaataga catagcttta
     1441 ttgaaaatgg ttttttcacc gtctgacgtt atcggtacta aatgacggca gtgagaactt
     1501 tcagctttcg gaattctaat gagtatttct ttcgagtgaa ctcaaacaat tgtatcacta
     1561 atgaacaaaa taatataaaa cagactttga aaaacgtaac cactttaatc tgtactttct
     1621 tgctattgtc aagcagtgct tttgctcaaa caagctatcc taaaggcgga tacaaatcct
     1681 ttgaag
//
LOCUS       DAOOOL010000294         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9319_length_1667_cov_27.593672, whole genome shotgun sequence.
ACCESSION   DAOOOL010000294 DAOOOL010000000
VERSION     DAOOOL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9319_length_1667_cov_27.593672"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKU82_12820"
     CDS             <1..819
                     /locus_tag="PKU82_12820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996305.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOZ83793.1"
                     /translation="EYFRDTGRPALIVYDDLSKQAVAYREVSLLLRRPPGREAYPGDV
                     FYLHSRLLERAAKINASDSIAQQMNDLPDSLKGKVKGGGSLTALPIIETQAGDVSAYI
                     PTNVISITDGQIFLEANLFNSGVRPAINVGISVSRVGGNAQIKSMKKVAGTLKLDQAQ
                     YRELEAFAKFGSDLDAATKNVIDKGARNVEILKQGQYSPLRVEQQVAIIYLGTKGLLR
                     NVDVKKVREFETNYLSVLEATQKTILDRLKKGEFDDSITSVLEKVAADLAPKFS"
     gene            837..>1667
                     /gene="atpG"
                     /locus_tag="PKU82_12825"
     CDS             837..>1667
                     /gene="atpG"
                     /locus_tag="PKU82_12825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOZ83794.1"
                     /translation="MANLKEVRLRIASVNSTQQITKAMKMVSAAKLRRAQDAIMQMRP
                     YAAKLREILENLSQSLSSDGSPFTKVRAANNILIVSISSNRGLAGAFNSSVIKATSRL
                     INETYQKQTKEGKVKVLAIGRKSGDFFAKNQNVYAGNENSLYNALNFTNTAVIAERIM
                     DEFAKEKWDRVILVYNQFKNAAVQILQTEQLLPIIPPATQEKSKTVSDYIFEPNKESI
                     VSDLLPTAVKTQLFKAVLDSHASEHGARMTAMNKATDNAGELIKDLRLQYNKARQAAI
                     T"
BASE COUNT          549 a          320 c          342 g          456 t
ORIGIN      
        1 gaatatttcc gtgataccgg tcgccctgca ttgattgttt atgatgattt gtcaaaacaa
       61 gcggttgcat atcgtgaggt atctttgttg ttaagaagac ctcccggacg tgaagcatat
      121 cccggtgacg tattttattt gcatagccgc ctgcttgagc gtgctgcaaa aatcaatgca
      181 tcagacagca tagcgcagca gatgaacgac ctacctgatt cacttaaagg aaaagtaaaa
      241 ggtggcgggt cattaacagc tcttccaatc atcgaaactc aggcaggtga cgtctctgca
      301 tatattccaa caaacgtaat ttctattact gacggtcaga tattcctcga ggcaaacctg
      361 tttaacagtg gtgtgcgtcc tgccatcaac gtaggtatct ctgtatcacg tgttggaggt
      421 aatgcacaga ttaaatcaat gaaaaaagtt gcaggaacat taaaactcga tcaggcacag
      481 taccgcgagt tggaagcttt tgcaaaattc ggttctgacc ttgatgctgc tactaaaaac
      541 gtaatagata aaggtgcccg taacgttgag attcttaaac aaggacagta ctcaccattg
      601 cgtgttgagc agcaggttgc cataatttat cttggaacaa aaggcttgtt acgtaatgtt
      661 gatgttaaga aagtaagaga gtttgaaact aattatctga gcgttctcga agcaacacaa
      721 aaaacaattc ttgatagatt gaaaaagggt gaatttgatg acagcataac ttctgtactt
      781 gaaaaagttg ctgcagacct tgctccgaaa ttttcttgat aattcttaat taaaaaatgg
      841 ccaatttaaa agaagtacga ctgcgcattg cttcggtaaa ttctactcag caaattacca
      901 aggcaatgaa aatggtttct gctgctaaat tacgcagagc acaggatgca attatgcaga
      961 tgcgtcctta tgctgccaaa cttagagaga tacttgaaaa cctttcacaa agtttgtcat
     1021 cagacggaag cccattcacc aaagtgcgcg cagcaaataa tattctcatc gtttctattt
     1081 cttcaaaccg tggtcttgcc ggtgcattta actcatctgt aataaaagca acatcaagat
     1141 tgattaatga gacctatcag aaacagacta aagaaggtaa agtaaaagtt cttgctattg
     1201 gaagaaagtc aggtgacttt tttgcaaaga atcaaaacgt ttatgccggt aatgaaaaca
     1261 gcctgtacaa tgcattgaat tttacaaaca ctgctgtaat cgctgaacgc attatggatg
     1321 agtttgcaaa agaaaagtgg gacagagtga ttttggttta taatcagttt aaaaatgctg
     1381 ctgtacaaat attgcagaca gaacaactgt tgccgataat tcctcctgct acgcaagaaa
     1441 aaagcaaaac agttagtgat tatatttttg aacccaataa agaatcaatt gtaagcgact
     1501 tattgcctac tgccgttaaa acccaattgt ttaaagcagt gcttgattct catgccagtg
     1561 aacatggtgc ccgaatgact gccatgaata aggccaccga caatgcaggg gaattaatta
     1621 aagaccttcg tctgcaatat aacaaagcca gacaggcagc tatcaca
//
LOCUS       DAOOOL010000295         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9335_length_1666_cov_11.525140, whole genome shotgun sequence.
ACCESSION   DAOOOL010000295 DAOOOL010000000
VERSION     DAOOOL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9335_length_1666_cov_11.525140"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKU82_12830"
     CDS             complement(<1..715)
                     /locus_tag="PKU82_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOZ83795.1"
                     /translation="MQITKEKVLEALSYVQEPDLKKDLVTLNMIKDIEIEGQNVSFTV
                     ILTTPACPLKAEIERACKNAIVHFVDKDAVVTVNMTANVTTQRSMNNEMMKGVKNIIA
                     VASGKGGVGKSTVAANLAVSLAKTGAKVALVDADIFGPSVPIMFGSEHEHPFIEEVNG
                     RQLMIPVEKHGVKLISIGYLADPSQAIVWRGPMASKALKQLFSDVNWGEIDYMIVDLP
                     PGTSDIHLTLISAVPLTGAV"
     gene            complement(746..1105)
                     /locus_tag="PKU82_12835"
     CDS             complement(746..1105)
                     /locus_tag="PKU82_12835"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOZ83796.1"
                     /translation="MKTSDSFNADEISFFEKVYQVVRLIPKGKVTSYGAIAKYLGSAQ
                     SSRMVGWAMNNSSRIKPKVPAHRVVNRNGMLTGKHFFGANDAMQHLLEKEGIKVSDDK
                     IIDFKKHFWDPSVELML"
     gene            complement(1168..>1666)
                     /locus_tag="PKU82_12840"
     CDS             complement(1168..>1666)
                     /locus_tag="PKU82_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83797.1"
                     /translation="SFTETATSYQLELRYGNAATGAAMTNNIAVDPLFTDSTYHLASH
                     TFTPANSGVYNIGINGFGSGVDNSIRVDDASMVVVTGIKETDLSSNVTLMPNPATTEL
                     LVSAKLYGNSTLTVYNQLGQEVIKQNYNEPFRITLDIQSLKKGVYTLKISNADGVVVN
                     KFVKQ"
BASE COUNT          509 a          330 c          287 g          540 t
ORIGIN      
        1 ctactgcacc tgtcagaggt actgcggaga ttaatgtaag atgaatatcg cttgttcccg
       61 gaggaaggtc aacaatcata taatctattt cgccccagtt aacgtcacta aaaagttgtt
      121 tcaatgcttt tgatgccata ggtcctcgcc aaacaattgc ctgcgatgga tctgcaaggt
      181 aaccaattga aataagtttt acaccatgct tttctaccgg tatcattaat tgtcttccgt
      241 ttacttcttc aataaaagga tgctcatgct ccgagccgaa cataattggc acactaggcc
      301 caaatatatc ggcatcaaca agggcaactt tagcaccagt tttagctagt gaaactgcta
      361 aattagcagc aacagtagat ttacctacac cacctttgcc ggaggcaaca gcaataatat
      421 ttttaacgcc tttcatcatt tcattattca tgctgcgctg tgtagtaaca ttggcagtca
      481 tatttactgt tacaacagca tctttatcta caaaatgaac tatggcattt ttacaggcgc
      541 gctcaatttc agctttcaga ggacaagctg gagttgtaag gattacggta aaactaacat
      601 tctgcccttc aatttcaata tctttaatca tgttcagtgt caccaaatct tttttaagat
      661 caggttcctg tacataactt aaagcttcta atactttttc ttttgtaatc tgcatggtgc
      721 aaagataatg gttgtaaatg agtgtttata gcatcaattc aacagatgga tcccaaaagt
      781 gttttttaaa atcaattatt ttatcgtctg atacttttat gccttctttt tcgagcaaat
      841 gttgcatggc atcattagca ccaaaaaagt gttttcctgt gagcatgcca tttcgattta
      901 cgactctgtg tgcaggaacc tttggtttta tccgactact attattcatc gcccaaccaa
      961 ccatacgtga tgactgtgcg ctgccaagat acttggctat ggcaccataa ctagtcactt
     1021 ttcctttcgg aattaaacga actacctgat atactttttc aaaaaaagaa atctcatcgg
     1081 cattaaagga atctgatgtt ttcatacagg tataaaaata caaaaaccac cgactgaaag
     1141 ccggtggttt ttgtataaaa agattaatta ttgttttaca aacttattta ctacaacacc
     1201 atcggcattg cttattttaa gtgtgtaaac acccttcttc aaagattgaa tatctaatgt
     1261 gatgcggaaa ggctcattat agttttgctt aataacttcc tgacctaatt gattataaac
     1321 agtaagtgtt gagttgccat atagtttagc agaaaccaat aattctgttg tagcaggatt
     1381 tggcataagt gtcacattgc tgcttaaatc agtttctttt atacctgtta ctactaccat
     1441 agaagcatcg tcaactctga ttgaattgtc tacaccagaa ccaaaaccat ttataccgat
     1501 gttataaaca cctgagtttg ctggggtgaa tgtgtgagaa gccaaatgat aagttgaatc
     1561 agtaaacaat ggatcaacag caatattatt ggtcatggca gcacctgtgg ctgcatttcc
     1621 ataacgcagt tctaattgat atgaagttgc agtttctgta aaacta
//
LOCUS       DAOOOL010000296         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9429_length_1653_cov_50.110763, whole genome shotgun sequence.
ACCESSION   DAOOOL010000296 DAOOOL010000000
VERSION     DAOOOL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_1653_cov_50.110763"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..458)
                     /locus_tag="PKU82_12845"
     CDS             complement(147..458)
                     /locus_tag="PKU82_12845"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOZ83798.1"
                     /translation="METVSMLIHVGNRQLNLKVPAADQENILKAEKLLNDKIAALRKS
                     YAVNDNQDILAMCAFSIAGELISQQASGKKSENTRMEKLESLDALLNGFLAQPELFNK
                     Y"
     gene            complement(466..801)
                     /locus_tag="PKU82_12850"
     CDS             complement(466..801)
                     /locus_tag="PKU82_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83799.1"
                     /translation="MDQQFEKIKAIESKIQKLIELNKNLAEEKAMYEDENRQLTEQVK
                     SLTEKMRQHEQKIDMQGQQLTQLEETNKSLNLATNETTETKEENKFLKSRIDEYVQEI
                     DKCLALLDK"
     gene            1086..>1653
                     /locus_tag="PKU82_12855"
     CDS             1086..>1653
                     /locus_tag="PKU82_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOZ83800.1"
                     /translation="MTKYFFTLIFSSAILFAQAQKTPSKIELVHANTLEGDDALGKDV
                     RRLLGDVIFKQENTFMYCDSAYLYATTNSMDAFGNVRIEQGDTISLTGDILKYNGNSR
                     QARLLNNIVFSDRKMMLVSQNLNYNLDTGIADYVDGGQIVDGTDTLTSRFGTYFSNQK
                     KLAFKKDVVLRHPGHRMETDTLVYGTVSK"
BASE COUNT          482 a          306 c          299 g          566 t
ORIGIN      
        1 tttataccct ttaacaggat gtgattaaac tttcattctg caaaacacga acccaatctt
       61 aagtctaaag atttaaattc agtttaacgt tttagaaaaa gcagaatgaa caggctgcac
      121 agaaaggcag ctataattat ggtttattag tatttattaa acaactccgg ctgagccaga
      181 aaaccgttta ataatgcatc cagcgactct aatttttcca ttcgagtatt ttctgatttc
      241 ttgccggaag cctgttggct tataagttca ccggcaatac taaatgcaca cattgccaga
      301 atatcctgat tgtcattgac agcatacgac ttgcgcaatg cagcaatctt atcattcaaa
      361 agcttttcgg cttttaagat attctcttga tcggcagcag gaaccttcag gttcagttgt
      421 ctgttgccga catgtataag catggatact gtttccattt tgttcttact tatccaataa
      481 tgcaagacac ttgtcaatct cctgcacgta ttcgtcaatt cttgatttca aaaatttatt
      541 ctcttctttc gtttccgttg tttcgttggt ggctaaatta agcgatttat ttgtttcctc
      601 caactgagtc aactgttgcc cctgcatatc aatcttttgc tcatgctgac gcatcttttc
      661 tgtaaggctt ttaacctgct ctgtcaactg acggttttca tcttcataca tggctttttc
      721 ttcggccagg tttttattga gttcaataag tttttgaatt tttgattcaa ttgctttgat
      781 tttttcgaat tgctgatcca taattatttg attaattggt ttttgattca gtaaaaatat
      841 tattcatgac tgtattacaa ccgcgcctaa ctaaaaatta gcaacaatac tttgttaatt
      901 agtcgaaaga aaagcaactt gaccttaaat acaagaatta aatcagaaat tctatgttaa
      961 aattctatta aagcagcttt gattatttgg ttctaaaaca gttgtttgtg tggccaaagc
     1021 gaagttgttg cattaaacat tgctgccact gagattgatt gaaacctctt atttttgcca
     1081 acttcatgac caagtatttt tttacactta ttttttcttc tgccattctt tttgctcagg
     1141 ctcaaaaaac accttcaaaa atagaattgg tacatgccaa tacattagag ggtgatgatg
     1201 ctttaggaaa agatgtgcga aggcttttgg gcgatgttat ttttaaacag gaaaatacat
     1261 ttatgtactg cgacagcgcc tatctctatg ctacaacaaa cagtatggat gcttttggaa
     1321 acgtgcggat tgaacaggga gataccatca gtcttacggg tgacatactg aagtataacg
     1381 gcaacagcag gcaggctcgt ttactcaaca atattgtttt tagcgacaga aaaatgatgc
     1441 tggtttcgca aaacctcaat tataatcttg atacaggcat tgcagattat gttgatggcg
     1501 ggcagattgt tgatggcaca gatactttaa ccagtcgctt tggcacatac tttagtaatc
     1561 agaagaagtt agcctttaag aaagatgttg tgctcaggca tcccggacac agaatggaaa
     1621 ccgatacctt ggtttatgga actgttagta agg
//
LOCUS       DAOOOL010000297         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9529_length_1641_cov_38.607188, whole genome shotgun sequence.
ACCESSION   DAOOOL010000297 DAOOOL010000000
VERSION     DAOOOL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9529_length_1641_cov_38.607188"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKU82_12860"
     CDS             <1..419
                     /locus_tag="PKU82_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ83801.1"
                     /translation="MKLDALNEWSSVVALTYIAAQRVFPHYNDMGDAKSLLESITRSF
                     GYHYGMAKKVRVNTISQSPTATTAGSGIKGFNAFYNYADVMSPLGNASADECANYTVM
                     MFSDFTRKVTMQNLFHDGGFSFTGVSLNAMEKFMEP"
     gene            636..1517
                     /gene="sucD"
                     /locus_tag="PKU82_12865"
     CDS             636..1517
                     /gene="sucD"
                     /locus_tag="PKU82_12865"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOZ83802.1"
                     /translation="MSVLVNKDSKIIVQGFTGTEGTFHAGQMIEYGTKVVGGVTPGKG
                     GTTHLDRPVFNTVADAVKQTGADVSIIFVPPAFAADAIMEAAQAGIKLIVAITEGIPT
                     KDMIQVRDYLRDKEARLVGPNCPGVITAGECKVGIMPGFVFQKGRIGIVSKSGTLTYE
                     AADQVVKAGAGISTAIGIGGDPIIGTTTKEAVELFMNDPETDGIIMIGEIGGNLEAEA
                     ARWIKNNSKKPVVGFIAGQTAPPGRRMGHAGAIVGGADDTAAAKMKIMAECGIHVVAS
                     PAEIGETMVRVLSKVSA"
BASE COUNT          497 a          367 c          345 g          432 t
ORIGIN      
        1 ccatgaaact cgatgccctc aatgagtgga gtagtgtagt ggcactcacc tacattgcag
       61 cacaacgtgt cttccctcat tacaatgata tgggcgatgc aaaatcgctt ttagaaagca
      121 tcacacgcag ctttggctat cattatggca tggcaaaaaa agtaagagta aacaccattt
      181 ctcaatcgcc cactgcaaca actgcaggca gtggcataaa aggtttcaac gccttctata
      241 actatgccga tgtaatgtcg cctcttggca atgcctctgc cgatgaatgt gcaaactaca
      301 ctgtaatgat gttttccgat ttcacacgca aggtaaccat gcaaaatctc tttcacgatg
      361 gtggcttctc cttcaccggt gtcagcctca atgcaatgga aaaatttatg gagccatagg
      421 ctcattatat ataaatcatc tcaccattgc attctaaatt gtgagatgat tttttttacg
      481 attcaccatc accaaaaatt attaaatcct acgctgtaaa caatcaaaat tttaagccta
      541 attattaaat caataagcac cctgctttat accgtcagtc acattattca aaaataaaat
      601 ttacttttgc gcaacttttt aattttcaat aagcaatgag cgttttagtc aataaagatt
      661 caaaaattat tgttcaggga ttcaccggaa cagaaggcac ttttcatgca ggacaaatga
      721 tagagtatgg taccaaggtt gttggtggtg taacccccgg caaaggcgga acaacacatt
      781 tagaccgacc tgttttcaat accgttgccg atgctgtaaa acaaaccggt gccgatgtaa
      841 gcatcatttt tgttcctccg gcttttgctg ccgatgccat tatggaagct gcacaggctg
      901 gcataaaact cattgttgcc atcacagaag gcatccctac taaagatatg attcaggtgc
      961 gcgactatct gcgcgacaaa gaagcgcgac ttgttggccc caactgcccc ggtgtaatta
     1021 ctgccggtga gtgtaaggtg ggtattatgc ccggctttgt atttcagaaa ggacgcatag
     1081 gcattgtctc taaatcagga acactaacct acgaagctgc cgaccaggta gtaaaagctg
     1141 gtgcaggcat ttctactgcc attggcattg gtggcgaccc cattatcggt accaccacaa
     1201 aagaagcagt agaacttttt atgaacgacc ccgaaaccga tggcatcatc atgattggcg
     1261 aaattggtgg taacctcgag gcagaagctg cacgatggat aaaaaacaac agcaaaaaac
     1321 ctgttgtagg tttcattgcc ggacaaacag cccctcccgg ccgcagaatg ggacatgccg
     1381 gagccatcgt tggtggtgct gacgatactg cagcagccaa aatgaaaatt atggccgagt
     1441 gcggcattca cgttgttgca tcgcctgcag aaattggcga aaccatggtg cgtgtactaa
     1501 gcaaagtatc tgcatagaac accatgcttt agcatgtaga aagtgattaa gttattatca
     1561 gccacagctg acaggggtga atactcaaat tagataattt gataattaga taattagata
     1621 actctgaatt cttgcataca c
//
LOCUS       DAOOOL010000298         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9642_length_1627_cov_62.118957, whole genome shotgun sequence.
ACCESSION   DAOOOL010000298 DAOOOL010000000
VERSION     DAOOOL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9642_length_1627_cov_62.118957"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKU82_12870"
     CDS             <1..509
                     /locus_tag="PKU82_12870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ83803.1"
                     /translation="VAEFRKKYSIPDKPIVYIGNAHKQKGVYEAYHALKDLDVELIMT
                     GAENNASDLPIRFLSLNREEYITLLHASAIIVLMSKLMEGWNRVAHEALLCNTPVVGS
                     GMGGMGELLTKSKQIITTNDAIKENVVAVLKNRNKYSNVGYDFVKQFDMNYFNSAWQQ
                     TIENLIRR"
     gene            510..>1627
                     /gene="asnB"
                     /locus_tag="PKU82_12875"
     CDS             510..>1627
                     /gene="asnB"
                     /locus_tag="PKU82_12875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOZ83804.1"
                     /translation="MCGINGVFNFNKVNIGDENALIGLMNDTIRHRGPDDSGISEVNQ
                     RLFFGHRRLSIIDLSKAGHQPMRNEVGYEIVFNGEIYNYAELKKYLPDYKFKSDSDTE
                     VLLGLYEKFGESCLQYLNGMFAFAIWDPRKEQLFLARDRSGKKPLYYSFQNGVFAFSS
                     EVKALLKLPFIKTELDEEAFYHFLTFNVLAPPMTMFRNIKKFHPAHSMTIEKTGHFRY
                     QPYWNIEYSDYSNLTEEELKTQTLERLQKSVQYRMVSNVPVGAFLSGGVDSSALVAMM
                     SKMTNQPVKTYSIGFEGQPDFDELEHARKVSEMFGTQHFEKTVNPDEFKNFLPKIVDI
                     FDEPLADPTCVPIYFISQKARQEGTIVVLTGDGSDEIFA"
BASE COUNT          551 a          258 c          321 g          497 t
ORIGIN      
        1 ctgtcgcaga attcagaaaa aaatattcta ttcctgacaa gcctatagtt tatattggca
       61 atgctcataa acaaaaagga gtttatgaag cctatcatgc attaaaagat ttagatgttg
      121 agttaatcat gaccggtgca gaaaataatg catctgactt gccgatacgt tttttatcat
      181 taaacagaga agaatatatc accttgcttc atgcctctgc gattattgtg ctcatgtcaa
      241 agttaatgga aggatggaac cgggttgctc acgaagcatt actttgcaac acaccagttg
      301 tcgggtcagg aatgggtggc atgggtgaac ttcttacaaa atcgaaacaa ataataacaa
      361 caaatgatgc tataaaagaa aatgttgtgg cagttttaaa gaatagaaat aaatatagta
      421 atgttggtta cgactttgta aaacagtttg acatgaatta cttcaattca gcatggcaac
      481 aaacaataga aaacttaatt agaaggtaaa tgtgcggcat taacggagtt tttaatttta
      541 ataaagtcaa catcggtgac gagaacgctc taataggttt gatgaatgac actatcaggc
      601 atcgtggtcc ggatgatagt ggtatttctg aagttaatca acgcttattt tttggacata
      661 ggcgccttag tattatagat ctttcaaaag ccggtcatca acctatgaga aatgaagtcg
      721 gttatgaaat agttttcaat ggtgagattt acaattatgc agagttgaaa aaatatctgc
      781 ctgattataa atttaaatct gatagtgaca cagaagtatt gcttggactc tatgaaaagt
      841 ttggagaaag ttgccttcag tatctgaatg ggatgtttgc ttttgcaatt tgggatcccc
      901 ggaaggagca attattttta gcaagagatc gttcaggaaa aaaaccttta tattattcct
      961 ttcagaatgg tgtatttgcc ttctcttcag aggtaaaagc attgttaaaa ctaccattta
     1021 taaaaacaga attagatgag gaagcattct atcacttctt aacatttaat gtattggccc
     1081 ctccaatgac tatgttcaga aacattaaaa agtttcatcc tgcacatagt atgaccattg
     1141 aaaaaaccgg tcatttcaga tatcagcctt actggaatat tgaatatagt gattatagca
     1201 atcttacgga agaagagtta aaaactcaaa ctctggaacg attgcagaaa tctgttcagt
     1261 atagaatggt ttctaatgtt cctgtaggag catttcttag tggtggtgtt gactcaagtg
     1321 cattggtggc tatgatgtca aaaatgacaa atcagcctgt taaaacctac tctatcggtt
     1381 ttgaaggtca acccgatttt gatgaacttg aacatgcaag aaaagtttct gaaatgttcg
     1441 gcacacaaca ttttgaaaaa acagtaaacc cagacgaatt taaaaatttt ctgccaaaga
     1501 ttgtagacat atttgatgaa ccattggctg accctacctg cgttcccatc tatttcattt
     1561 ctcagaaagc aagacaggaa ggtacaatag ttgtgctaac cggtgatgga agtgatgaaa
     1621 tttttgc
//
LOCUS       DAOOOL010000299         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9718_length_1619_cov_119.822890, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000299 DAOOOL010000000
VERSION     DAOOOL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9718_length_1619_cov_119.822890"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKU82_12880"
     CDS             complement(<1..524)
                     /locus_tag="PKU82_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83805.1"
                     /translation="MLKKIVLTFLPLFISLLLNADTPLLKRVVCSYDDTTRLSFVSNA
                     NVFTERWDTLQQVKFWQEVIRQSADTALINVASTRCILHKTPFEDWKCQSESEKTAYK
                     KFINLANGLDTNTNLYVTFGKKEFYEYKRVIPVIGKSVEIFKNNKVDPWYAQTILLIE
                     NPGKNHNKSYVGANG"
     gene            complement(789..>1619)
                     /locus_tag="PKU82_12885"
     CDS             complement(789..>1619)
                     /locus_tag="PKU82_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOZ83806.1"
                     /translation="RNIHILNDQQFPIESFIQTDAAVNPGNSGGALVNLNGDLLGINA
                     AIASNTGSYTGYSFAIPANIVRKVTKDLLEYGKVQRGFIGVTLRDIDQQLAQNKGLKS
                     MQGVYIDGVVSGGAGQAAGIKPGDVVTKVGETTVNSASELQEQIGRFGPGDKVNLTLN
                     RDGTEKNYQIVLRDNSGGERVERANNNVNMMGAEFGEVTDKEKKQLGISNGVKVAGLQ
                     SGKLRSAGIREGFIITSIDNKKINSPDDIEAALANKKGGVLIEGVYPNGMRAYYGFGL
                     "
BASE COUNT          490 a          341 c          247 g          541 t
ORIGIN      
        1 ccattggctc caacataaga tttgttgtga ttttttccgg gattctcaat caatagaatt
       61 gtttgtgcat accaagggtc aactttattg tttttaaaaa tctcaacgga ttttccgatt
      121 acaggaataa cccttttgta ttcataaaac tcttttttcc cgaatgtaac ataaaggtta
      181 gtatttgtgt caagaccatt ggctaagttt atgaacttct tataggctgt cttttctgat
      241 tctgactgac atttccaatc ttcaaatggt gttttatgaa gtatgcatcg tgtagaggca
      301 acgttaatca gtgccgtatc agcggactgt ctgattacct cctgccagaa tttaacctgc
      361 tgaagtgtgt cccatcgctc tgtaaaaaca tttgcattag aaacaaaact caaccgggtt
      421 gtatcatcat aactacaaac aactcttttt aataaaggcg tgtctgcatt taacagcaag
      481 ctgatgaaca gtggtaaaaa tgtaagtact atttttttta acatcaattg catttatagt
      541 tatacggaaa ttaacataac ctgtttagac tttttttatt gttttcttgc atcttttgca
      601 aaggtaggct atttaaaata gaaatattaa gccactgcaa gttaaacaaa attcgttttc
      661 aacacaagag tattaactat tatttgaatt gtgagagaaa tactgcaatt tataagttgc
      721 ataaaatgca aagggagatt cattttcaga atcccccttc gctataaacc aaacctatga
      781 aaaactatct ataatccgaa tccataataa gctctcatgc cattaggata aacaccttca
      841 atcagcacac cacctttttt attggctaat gcagcttcaa tgtcgtcagg agaatttatt
      901 ttcttattat caatgctggt aattataaac ccttcgcgaa tacctgcact tctcaacttt
      961 cctgattgta aacctgcaac cttaacacca ttgcttatgc ctaattgttt tttctcttta
     1021 tcagtcactt ctccaaattc agcacccatc atgttgacat tgttattagc acgttcaaca
     1081 cgttcaccac cactgttgtc gcgcaatact atctgataat ttttttctgt accatcacga
     1141 ttcaatgtaa gattaacttt atcaccaggg ccaaagcgtc cgatttgttc ctgaagctca
     1201 cttgcagaat taactgtagt ctcgccaacc ttagttacca cgtcaccggg ttttattcct
     1261 gctgcttgtc cggcaccacc actcacaacg ccatcaatat aaacaccctg catagatttt
     1321 aatcctttat tctgtgctaa ttgctggtca atatcgcgta atgtaacacc aataaatcct
     1381 cgttgaacct taccatattc caataaatct ttagttacct ttcttacaat atttgcagga
     1441 atggcaaaag aatagccggt atatgaacct gtattggaag caatggcagc attgatacct
     1501 aacaaatcac cgttcaggtt tactaatgca ccaccactat taccgggatt cacagctgca
     1561 tcggtttgaa taaatgattc aatcggaaac tgctgatcat ttaaaatatg aatgttgcg
//
LOCUS       DAOOOL010000300         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9862_length_1604_cov_92.480955, whole genome shotgun sequence.
ACCESSION   DAOOOL010000300 DAOOOL010000000
VERSION     DAOOOL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9862_length_1604_cov_92.480955"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKU82_12890"
     CDS             <1..384
                     /locus_tag="PKU82_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOZ83807.1"
                     /translation="TVKKMIAKGKDISKSKVLVMGATFKEDVEDIRNSKVTDVVNEFK
                     SFGVTIEVTDPYASSDELMHEYGFGLVDKIANDYDAVVVAVSHKDYLKMDENYFKSIC
                     KPDGVIVDIKGFLRNKIKNLQYWSL"
     gene            451..1476
                     /gene="galE"
                     /locus_tag="PKU82_12895"
     CDS             451..1476
                     /gene="galE"
                     /locus_tag="PKU82_12895"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOZ83808.1"
                     /translation="MKVLVTGGTGYIGAHTVVELMNSGFDVEIIDNLNNSKEEVVDAI
                     AKITGKRPIWFNIDLCDASELKNYFSKYKPDAVVHFAAHKAVGLSVEQPLEFYRNNLL
                     SLINILQCCVDAKCNNFVFSSSCSVYADPDKLPIDENAPVKTAKSPYGNTKKIGEEII
                     TDTVKSSLLQAVSLRYFNPVGSHDSALIGELPTTMPLNVMPIITQVAIGKRKQFVVFG
                     NDYPTADGSCVRDYIHVVDVAKAHVTAVKRLLNKKNNSAYEVFNLGTGKGFSVLELIH
                     AFEKTTKQKLNYTIEGRRAGDVTAVYADTTLANTVLGWHTERDLNNMIRTAWNWELYL
                     AGRTDFI"
     gene            1521..>1604
                     /locus_tag="PKU82_12900"
     CDS             1521..>1604
                     /locus_tag="PKU82_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOZ83809.1"
                     /translation="MPKSTIIITGGAGFIGSHVVRRFVNNYS"
BASE COUNT          518 a          275 c          325 g          486 t
ORIGIN      
        1 accgtaaaga aaatgattgc caaaggcaag gatatttcta aatcgaaagt gctggtgatg
       61 ggtgctacat ttaaagaaga tgtagaagat atccgcaaca gcaaggttac ggatgtagtg
      121 aacgaattta aatcatttgg tgtaacaata gaagttaccg acccttatgc ttcttctgat
      181 gagttgatgc atgaatatgg ttttggtttg gttgataaaa ttgccaatga ttatgatgct
      241 gtagtggttg ctgtttcgca taaggattat cttaagatgg atgaaaatta ttttaagagt
      301 atctgtaaac ccgatggtgt tattgttgac ataaaaggat ttttaagaaa taaaatcaaa
      361 aatcttcaat actggagtct gtaaaccttt agtgcggcaa cgccctaacc gttatacaga
      421 aatattaacc cggtaaattt attgcatgta atgaaagttt tggtcaccgg aggcaccggt
      481 tatattggtg cacacactgt tgtagaactc atgaacagtg gttttgatgt agaaatcatt
      541 gacaacctga ataattcaaa agaagaagtt gttgatgcta ttgcaaaaat taccggcaaa
      601 cgcccgatat ggtttaacat tgacctttgt gatgccagcg aattaaaaaa ctatttcagc
      661 aagtataaac cggatgcagt tgtacacttt gcagcacata aagcagtagg gctttctgtt
      721 gaacaaccat tggaatttta tcgaaacaac ctgctttcgc tcattaatat tttgcaatgt
      781 tgtgttgatg caaagtgcaa taattttgtt ttttcatcaa gttgctctgt ttatgctgat
      841 cctgataaat tacctattga tgaaaatgct cctgtcaaaa cagctaaatc gccttatggc
      901 aacaccaaaa aaattggaga agaaataata acagacactg taaaatcatc tttactgcaa
      961 gctgtttcac ttcgctattt caatcccgtt ggctcacacg atagtgcttt aatcggtgaa
     1021 ctgcctacaa ccatgccatt gaatgtaatg cccatcatca ctcaggtagc cattggtaaa
     1081 cgcaaacagt ttgtggtttt cggaaacgat tatccaacag ccgatggcag ttgtgtaaga
     1141 gattatatac atgtagttga tgtggcaaag gcacatgtta cagctgtgaa acgattgctc
     1201 aacaaaaaaa acaactctgc ttacgaagta tttaatctgg gaacaggaaa aggattttct
     1261 gtacttgaac ttattcatgc atttgaaaaa accacaaaac agaaattaaa ttataccatt
     1321 gaaggaagac gtgccggtga tgtcactgct gtttatgccg acacaacatt ggctaacacc
     1381 gtacttggat ggcatacaga gcgcgatttg aataatatga tacgtactgc ctggaactgg
     1441 gagttgtatc tggccggaag aacagacttt atctgagcta aaggttcttt attaagtttt
     1501 aattttttaa ttttaccctc atgccaaaaa gtacaatcat tatcacaggc ggtgccggat
     1561 tcataggttc gcatgttgtg cgcagatttg taaacaatta ttcc
//
LOCUS       DAOOOL010000301         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_9956_length_1593_cov_23.278934, whole genome shotgun sequence.
ACCESSION   DAOOOL010000301 DAOOOL010000000
VERSION     DAOOOL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9956_length_1593_cov_23.278934"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..>1593)
                     /locus_tag="PKU82_12905"
     CDS             complement(132..>1593)
                     /locus_tag="PKU82_12905"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOZ83810.1"
                     /translation="DNVGVHPTYNLGRTLTHEVGHWLNLIHIWGDDGGTCTGTDDVAD
                     TPNQASEHFGTPNFPTISCSNNPDGDMFMNFLDYTDDYAMNIFTAGQSLRMQDAVNNS
                     RAALLTSKGCMDTTTAIDVALLNVINVETIYCQGNITPSAVIKNKGAGTISSLTFSVK
                     LDDNTATNKTWTGSLVAGATATVALDPLTVPTGTHYLNVSVTQSGDGNVSNNIIVKRI
                     TVTGATALPYSQDFEAKRFPADNWLVLNADNDATFMRSRLASINGSSSLWFYAPLQTP
                     AGRKDIVQLEPFDLTTLAEPVLAFDVACLNDNSISTSASLKILASTDCGSTFSEIFEK
                     TGSALTTAEGSASNVFVPVAEQWRKDSVNLKALNSFNNVILRFEYTTGNANALFIDNL
                     QIDRLGVIYPDASNVNVSVYGQSAYGVLDFSVLLLKTVDFKVDIFDVAGRKVFSKSYS
                     GSAVCDRLSLHQLNAGVYFFRVTSGKDSKSVKLFFR"
BASE COUNT          460 a          389 c          273 g          471 t
ORIGIN      
        1 ataatatgaa agtcttaact ataaagtttt tttattcttg actctaaaca atcaatgacc
       61 tcaagtttca gtttgaaacc acctccgatt ttcaattcat aactctgcat tctgaattca
      121 aaaccaaact cctatctgaa aaacagcttc accgactttg aatccttgcc cgatgtaact
      181 ctgaaaaaat aaacccctgc atttaactga tgcaagctaa gcctgtcgca aacggcactg
      241 ccgctatacg actttgaaaa tacttttctt cctgcaacat caaaaatatc taccttaaaa
      301 tctactgtct ttaaaagcag cacactaaag tcaagcacac cataagcact ttgtccatac
      361 acgctcacat tcacattcga tgcatcagga taaataaccc caaggcggtc aatttgcagg
      421 ttatcaataa aaagggcatt ggcattgcct gtagtatatt caaaacgtaa aatcacatta
      481 ttaaaactgt tcaacgcttt cagattcaca gaatctttcc gccactgttc agcaaccgga
      541 acaaatacat ttgatgcact gccttctgct gttgttagtg cacttcctgt cttctcaaat
      601 atctcactaa acgtactgcc acaatccgtt gatgccaata tctttaaact tgcacttgta
      661 ctgatgctgt tgtcattcag gcaggctaca tcaaaggcaa gcactggctc tgcaagtgtt
      721 gtaaggtcaa atggctccaa ctgcacaata tcttttcttc ctgctggcgt ttgcaagggt
      781 gcataaaacc acaaagaaga acttccattt attgatgcta gcctgctgcg cataaaagtt
      841 gcatcattgt ctgcattcaa caccagccaa ttgtctgccg gaaatctttt cgcctcaaaa
      901 tcctgactgt agggcagggc agtggcaccc gtcaccgtaa ttctttttac aataatgttg
      961 tttgatacat tgccatcgcc actctgtgtc acactcacat tcagataatg tgttcctgtc
     1021 ggaactgtca acggatctaa tgcaaccgtt gccgttgcac ctgcaaccaa actacctgtc
     1081 caggttttat ttgttgctgt attatcatca agcttaacag aaaatgtaag cgagctgata
     1141 gttccggctc ccttattttt tatcactgcc gatggtgtaa tgttgccctg gcaataaata
     1201 gtttctacat taataacatt tagtaaggca acatcaattg ccgttgttgt gtccatacat
     1261 ccctttgatg tcagcaatgc cgccctgctg ttatttacgg catcctgcat gcgcaacgac
     1321 tgacctgctg taaaaatatt catagcatag tcgtcagtat aatcaagaaa gttcataaac
     1381 atatcgccat cggggttgtt gctgcaactg atagtcggaa aattgggtgt gccaaaatgc
     1441 tccgatgcct gattgggtgt gtctgcaaca tcatctgttc ctgtgcatgt gccgccatca
     1501 tcaccccata tatgtatcag attcagccaa tgcccgactt catgcgtcag cgtacgacct
     1561 aaattataag taggatgcac accaacatta tca
//
LOCUS       DAOOOL010000302         1579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_10084_length_1579_cov_35.730971, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000302 DAOOOL010000000
VERSION     DAOOOL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_1579_cov_35.730971"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKU82_12910"
     CDS             complement(<1..1286)
                     /locus_tag="PKU82_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83811.1"
                     /translation="MKRFDILIRSKMILLQIILFANSCLSQGYNHTWLLGYHPNVNNP
                     FDTMAQIDFNISSFSIVNYQRKIPFRGFTQANISDANGNLLMSSNGAWIANSTGDTMM
                     NGSFLGYDSYINANAQGLNITYAAIFIPYPDDSTKYVLFHQSLDSDGISYYSPAINYS
                     IIDITKDGGLGAVDSTQKYIAAFQDTMNGGIAACRHANGRDWWVVAQKHDTDTIYKIL
                     FTPTGIASITTQKLNVPKAWYSVPHLTFSPDGKKFVYQTYIPNQPDSSYILTFDFDRC
                     SGLFTTHNPVFVNRGYIWGLAFSPNSQFVYACTADKLYQLNLNTYNLDTIATYDGFNS
                     PYTWCCPTTFFNCYLAANGKIYITSGNAIQHIHEINYPDSTGTACDFQQHAIPIDCYH
                     RGSVPNHPNYYLGCDTTQTTCPCLITGINEIKQHNF"
     gene            complement(1283..>1579)
                     /locus_tag="PKU82_12915"
     CDS             complement(1283..>1579)
                     /locus_tag="PKU82_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOZ83812.1"
                     /translation="GYRKAATNEPANEVIKGNISIIPNPANEVITVTLSDDISGICKI
                     LFYDVVGKLILSKELDCNKKTHSLSVKNLSEGVYTVKAKIANQNSKQFKLIVVR"
BASE COUNT          487 a          269 c          283 g          540 t
ORIGIN      
        1 ttaaaattgt gttgcttaat ttcatttatg cctgttatca aacaagggca agtggtttgt
       61 gtagtatcgc agcctaaata ataattaggg tgattgggta cagaacctct atgataacaa
      121 tcaataggaa tagcgtgttg ttgaaagtca caggctgtgc ctgtactgtc aggataattt
      181 atctcatgta tatgttgaat tgcatttccg gaggtaatgt aaattttacc gtttgcagca
      241 aggtagcagt taaagaaagt ggtcggacag caccatgtat aaggcgagtt aaaaccatca
      301 taggtagcta tggtatctaa attatatgta tttaaattta actgatataa tttatctgcg
      361 gtgcaggcat atacaaactg tgaatttgga gaaaatgcca agccccagat atatcctcta
      421 tttacaaata caggattgtg cgttgtaaac aaccccgaac atcggtcaaa atcaaaagtt
      481 aaaatataac tgctgtctgg ttgatttgga atataggttt ggtaaacaaa ttttttaccg
      541 tcaggcgaaa aggttaagtg aggaacgctg taccatgcct tcggaacatt taacttttga
      601 gttgtaatgc ttgcaatgcc tgttggagta aaaagaattt tatagattgt atccgtatca
      661 tgtttttgag ccaccaccca ccagtctcta ccattggcat ggcggcaggc ggcaattcca
      721 ccattcatag tatcctgaaa agcagcaata tatttttgag ttgaatcaac tgcacctaaa
      781 ccaccgtctt ttgttatatc tataatgctg taatttatgg caggactgta ataagaaata
      841 ccatcactgt ctaatgattg atgaaataaa acatatttgg ttgaatcatc ggggtaagga
      901 ataaatatgg ctgcatatgt aatattcagc ccttgtgcat ttgcatttat atatgaatca
      961 taccccaaaa aacttccatt catcatggtg tcgccagtgc tgttggctat ccatgcacca
     1021 ttactgctca ttaataaatt tccgttggca tcactaatgt ttgcctgtgt aaaccccctg
     1081 aaagggattt ttctttgata atttactata ctgaaacttg aaatgttaaa atcaatttgt
     1141 gccatagtgt caaaaggatt gtttacatta ggatgatatc ctaagagcca agtatgatta
     1201 taaccttgcg ataaacaact gtttgcaaac aaaatgattt gcagcaaaat cattttgctt
     1261 ctgattagga tatcaaatcg tttcatcgta ctactatcaa cttaaactgt tttgaatttt
     1321 gattggctat ttttgctttt acagtgtata caccttcact taggttttta acactcagtg
     1381 aatgtgtttt tttattacaa tccaattctt ttgatagaat taatttgcca actacatcat
     1441 aaaacagtat tttacaaatt ccacttatat catcactcaa tgtaacagtt attacctcat
     1501 tggctggatt tggaattatg gagatgtttc cttttattac ttcatttgca ggttcattag
     1561 tagctgcttt tctatatcc
//
LOCUS       DAOOOL010000303         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_11019_length_1493_cov_69.682197, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000303 DAOOOL010000000
VERSION     DAOOOL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11019_length_1493_cov_69.682197"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKU82_12920"
     CDS             complement(<1..268)
                     /locus_tag="PKU82_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83813.1"
                     /translation="MKSIQKHLINLMIAGMGITLFAACGGSAPKEEKAAESKEMNQYY
                     EEEKQKEDTKVADDGKGLGKFTDVKVDPKLDKQMADAGKSVYDVK"
     gene            complement(488..655)
                     /locus_tag="PKU82_12925"
     CDS             complement(488..655)
                     /locus_tag="PKU82_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83814.1"
                     /translation="MLFAIFSLHNFREPGNLDAIDDRHCKKKDRLRALYIITGAIVVA
                     LLYTWLISVFC"
     gene            985..>1493
                     /locus_tag="PKU82_12930"
     CDS             985..>1493
                     /locus_tag="PKU82_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HOZ83815.1"
                     /translation="MMKKTAVIFFILFSQLITVFGQSQDSLMLRKIYNESLEHGQAYN
                     YLKVLCKQIGHRLSGSPEAEKAVKWAEKSMKDFGADTVFLQNIMVPHWVRGEKESASI
                     ISSKNNQSTPLNVLALGGSVGTNGKTIAANVVEVTSLDELDKLGTSKCAGKIVFINQH
                     MDATKIMTF"
BASE COUNT          509 a          244 c          254 g          486 t
ORIGIN      
        1 attttacatc ataaacggat ttacctgcat ctgccatttg tttatcaagt tttggatcta
       61 cttttacatc agtaaacttt ccaagaccct ttccatcatc tgctacctta gtgtcttctt
      121 tttgtttttc ttcttcgtaa tactgattca tttcttttga ctcagcagct ttttcttctt
      181 ttggcgcaga accgccacaa gcagcaaaaa gggttatgcc cattccggca atcattaaat
      241 tgattaagtg tttttgaatt gatttcatat tgatgatttg aattaaaatt ttacgtgaca
      301 aaagtataaa taattaacag ggtgttcaaa ataagttaat atgattgatg tcacataaac
      361 tattgataat tacatgtagc gtaaactgct tttcagcatg tttccttaat gaatctaaac
      421 cccctaaaaa agtatcagga taaaataaat atactgtgtg ttttgctgtc cgttttagca
      481 atacatttta gcagaaaaca gaaatcaacc aggtatagag taatgcaaca actatagctc
      541 cagtaataat atataaagct ctcaaacggt ctttcttttt acaatgtctg tcatcaatag
      601 catcaagatt tccaggttcg cgaaaattat gcagactgaa aatcgcaaac agcacgcaaa
      661 atctgctaac aacttcaacg aacagaatta atgttgctaa tgttgtcagg taaaggagat
      721 aaaaaatcca aatgctatga atgaaatgaa cagttattgt taataatata ctgatcagta
      781 gaaacgaaat cagaaatttt tgccttattc tgatctcagc ttcacctatg ttacaacacc
      841 cggctttata ctgatttgtg tacattgtat ctattattaa accttaacat caaagttcca
      901 ttattgttct gtaaaaatat tgaatgaagt gaaaatatta tagactctct ttatttactt
      961 tcacagccaa aatagggaaa gagaatgatg aaaaaaacgg cagttatttt ttttatttta
     1021 ttcagtcagt tgataactgt ttttggtcag tcgcaggatt ctttgatgct tcgaaaaatt
     1081 tacaatgaat cgttagagca cggacaggca tataattacc ttaaagtgtt gtgtaaacaa
     1141 attggacata ggcttagcgg ttcaccagag gctgaaaagg cagtaaagtg ggccgaaaaa
     1201 tcaatgaaag attttggtgc agacacggtt tttcttcaga acatcatggt accgcattgg
     1261 gtaagaggtg aaaaagaaag tgcttctata atttcttcaa aaaacaatca gtcaacacca
     1321 ttaaatgtgc ttgctttagg cggaagtgtt ggaacaaatg gaaaaacgat tgcagccaac
     1381 gttgtggagg taacttcatt agatgagcta gataaattag gtacttcaaa atgtgcaggt
     1441 aaaatagttt ttatcaatca gcacatggat gcaactaaaa ttatgacatt tga
//
LOCUS       DAOOOL010000304         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_11396_length_1460_cov_32.449822, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000304 DAOOOL010000000
VERSION     DAOOOL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11396_length_1460_cov_32.449822"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1460)
                     /locus_tag="PKU82_12935"
     CDS             complement(<1..>1460)
                     /locus_tag="PKU82_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOZ83816.1"
                     /translation="PSITFLKPISSAISIGTGGPFGAEGPIIATGGALGSTLGQLMRI
                     TPNERKILLAAGATAGMAAIFGSPVAGILLAIELLLFEFSPRSFVPVALACITGSAGH
                     HLFFGEGVVFPMQQQVENASNAALFFYSAMGVVVGVLAVATTKIVYWIEDMFEKLPVH
                     WAWWPAIGGLAVGIVGYFEPRSLGVGYNNITDLLAGSMSLQLVVSLCLLKFVSWAISL
                     GSGTSGGTLAPLLTIGGALGVMAGMVGQFWFPLADISIPLAALVGMSAMFAGASRAFL
                     TSIVFALETTGQSNALLPLLASCTSSYFVSYFLMENTIMTEKIARRGVYTPSTYEPDV
                     LEKIKVDQLATDNGITISDDMKIGEVRNWLQQEPDLQANYYIISSADGKYIGLLSSTT
                     IFSSHHQPDLLIGSLVKRTQVFVSNTDTLKTAVQTMAKENVDILPVVSEQEIIGILTY
                     QHIISVYNHGIDELETKQKHISLKRQRLKILLRGQK"
BASE COUNT          480 a          308 c          263 g          409 t
ORIGIN      
        1 atttttgtcc gcgcagaagt atttttaatc gttgacgttt taaagagata tgtttttgtt
       61 tagtttccaa ttcatcaata ccatgattat agactgatat gatgtgctga taggttagaa
      121 taccaattat ttcttgttcg gaaacaacag gcagaatatc cacattttcc tttgccatag
      181 tttgaactgc agtctttagt gtatcagtat tactcacaaa aacctgtgtt cgtttaacta
      241 aagatccaat caataaatcc ggttgatgat gtgaactgaa aatggttgtt gaactgagca
      301 atccaatata tttaccatca gcactagaaa tgatataata atttgcctgt aagtctggtt
      361 cttgctggag ccaatttctg acttcaccta ttttcatatc atcacttatt gtaattccat
      421 tgtctgtggc taactgatct acttttattt tttctaaaac atcgggctcg taggtgctgg
      481 gtgtataaac tcctctacgt gctatttttt ctgtcataat tgtgttttcc atcagaaaat
      541 aggaaacaaa ataagatgat gtgcatgatg caagcagggg caataatgca tttgattgtc
      601 ccgttgtttc caaagcaaaa actattgatg ttaaaaatgc acgagaagca cctgcaaaca
      661 tagcagacat accaacgagt gcagcaagtg gaatactgat atctgccaaa ggaaaccaaa
      721 actgtcctac cattccagcc ataacaccca atgcgcctcc aatagtaagt aatggagcaa
      781 gcgtacctcc ggaagtgcca ctaccgaggg atattgccca agaaacaaat tttaaaaggc
      841 ataatgaaac tactaactgc agcgacatgg aaccggcaag taagtcggta atgttattat
      901 aaccaacacc taatgaacgc ggttcaaagt agccaacaat gccaactgcc agaccaccaa
      961 ttgcaggcca ccatgcccaa tgtacaggca atttctcaaa catatcttca atccagtaaa
     1021 caatttttgt tgttgctact gccaaaacac ctacaacaac acccattgcc gaataaaaaa
     1081 acaaagctgc atttgaagca ttctcaacct gttgttgcat gggaaagaca acgccttcgc
     1141 caaaaaataa atgatgtcct gcagaacccg ttatgcaagc caaagcaaca ggcacaaaag
     1201 agcgtggcga aaattcaaat aacaaaagtt ctattgccaa taaaattcct gcaactggac
     1261 tcccaaaaat tgcagccata ccggcagtgg cacctgcagc taaaagtatt tttctttcat
     1321 taggtgtaat gcgcattaac tgacctaatg ttgagcctaa tgcacctccc gtagcaatga
     1381 taggtccttc ggcaccaaat ggacctcctg ttcctattga aatagcagaa gaaattggtt
     1441 ttaaaaatgt gatagatggt
//
LOCUS       DAOOOL010000305         1408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_12049_length_1408_cov_174.992609, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000305 DAOOOL010000000
VERSION     DAOOOL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12049_length_1408_cov_174.992609"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKU82_12940"
     CDS             <1..192
                     /locus_tag="PKU82_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83817.1"
                     /translation="DSDCSEQEFMNKYDFMVQDKRLTVEYLKIKYKWMKGEATWRDVC
                     VEYFKTNAYLTSVKNIKDV"
     gene            185..511
                     /locus_tag="PKU82_12945"
     CDS             185..511
                     /locus_tag="PKU82_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83818.1"
                     /translation="MYNIKLPELVQSLKNSVYVCKDYVPYQITKNGTFNVDKNEYDLE
                     LGWLQKHEKTDIIGETIDTYVVRIIHGNHGIWIGDIVVSLYYFLPLGVHKSRFVSWNN
                     NQLKLI"
     gene            516..713
                     /locus_tag="PKU82_12950"
     CDS             516..713
                     /locus_tag="PKU82_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83819.1"
                     /translation="MAKEIKEPVKYTCTSCGGDAYIAFVPETIKRGKLKGQQRAGWNG
                     IVKPGERICLPCGKKRQLKIL"
     gene            724..1104
                     /locus_tag="PKU82_12955"
     CDS             724..1104
                     /locus_tag="PKU82_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83820.1"
                     /translation="MDLSEAFEHLLENVDKNKYTKGTVASWKNRFNANKLSYEKMHEL
                     LLDNNYKCFKSDEVWLQPGSDKPYEFAVLDKDEILIYKEIFASSKSEATNKLKAENYI
                     LLNDLSNKKRIESIVNLLNVFQNS"
     gene            1199..>1408
                     /locus_tag="PKU82_12960"
     CDS             1199..>1408
                     /locus_tag="PKU82_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83821.1"
                     /translation="MSVLKWSEFEGYENHKWDGTVDPFMSACKALTARKWVGIESATS
                     VGKTYCLPRIIYWFLDTFPNSLVITT"
BASE COUNT          512 a          196 c          310 g          390 t
ORIGIN      
        1 gatagtgatt gctccgagca ggagtttatg aacaagtacg attttatggt acaggataaa
       61 cggctgacag ttgagtatct gaagataaaa tacaaatgga tgaaaggaga ggccacatgg
      121 cgtgacgttt gtgtggaata cttcaaaaca aatgcttatt taacaagtgt aaaaaatata
      181 aaagatgtat aatattaaac tacctgaatt ggtgcagtca ttaaaaaatt cagtttacgt
      241 ttgcaaggat tatgtgcctt atcagattac caaaaacggt acttttaacg ttgataaaaa
      301 tgagtatgac ttagaattag gttggttgca gaaacacgaa aaaacagata tcattggaga
      361 aacaatagat acttatgtcg ttagaattat tcatggaaat catggaatat ggattggtga
      421 tatagtagta agtttatatt actttttacc tttaggagta cataaaagca ggtttgtaag
      481 ttggaataat aatcagttaa aattaatata gtattatggc aaaagaaata aaagagccgg
      541 ttaaatatac ctgcacaagt tgtggtggtg atgcatacat agcgtttgta ccggaaacta
      601 taaagcgagg aaaattgaaa ggacaacaga gagcaggttg gaacggtata gttaagccgg
      661 gagaacgtat ctgtctgcca tgcggaaaaa aacgtcaact taaaatttta taaaataata
      721 actatggatt tatctgaagc attcgaacat ctactcgaaa atgtagataa gaacaaatat
      781 accaaaggaa cggtagcaag ttggaagaac cgatttaatg cgaacaagct atcctatgaa
      841 aaaatgcatg agttgttgct cgataacaat tacaagtgtt ttaagagcga tgaggtttgg
      901 ttgcaacctg gctccgacaa gccgtatgag tttgcggtat tggataagga tgagattttg
      961 atttacaaag aaatattcgc atcttcaaag tctgaagcaa caaacaaatt gaaagcggag
     1021 aattatatcc tgctgaatga cctaagtaat aaaaaacgga ttgaaagtat agtaaatttg
     1081 ctcaatgtat ttcaaaactc gtaaagaatt agcattaatc gaattagcca aacgtcgaaa
     1141 gattggtaac taccaagaac ggcctgattt atggttgagt gaaagatttg gtgagccatt
     1201 gagcgtactg aagtggagtg aatttgaggg atatgagaac cataaatggg acggcaccgt
     1261 agatccgttc atgtctgcct gtaaagcatt gacggcccgg aagtgggttg gaattgaaag
     1321 cgcaacttca gtaggtaaaa catattgcct accacgcatc atttattggt ttttagacac
     1381 gttcccaaac tcgcttgtaa ttactact
//
LOCUS       DAOOOL010000306         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_12899_length_1348_cov_33.433101, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000306 DAOOOL010000000
VERSION     DAOOOL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12899_length_1348_cov_33.433101"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKU82_12965"
     CDS             complement(<1..677)
                     /locus_tag="PKU82_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOZ83822.1"
                     /translation="MKWAYYDGQWVDAATPVLKLNNRGFIYGDGLFESIRCSRGKANF
                     LSLHIERLLAGVAVLNLKCDFITEDYLREIILEMNQRNNFTDGSKLRLLITRNAGGTY
                     LPEQNNASLCMLPITAVESEYTIPKCFIELGIYSEQQKPINKLSGFKNCNSLLYVLAS
                     IYAQEMKFDDCLILNSKNMLCEATASNVFWLKGNKVYTTPLSSGCVNGVMRKVIIQLT
                     SAIEQDIS"
     gene            complement(658..1170)
                     /locus_tag="PKU82_12970"
     CDS             complement(658..1170)
                     /locus_tag="PKU82_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOZ83823.1"
                     /translation="MINSNKAIFFDRDGVINDNTLYYTYKIADVVFNDGIFDAMKIFQ
                     DNGYLIFVVTNQSGIAKGVYTHNDVLAVHQFMSAEFKKKSITITNYYYCPHHPEKSKC
                     LCRKPDSLLLEKAIARYKVDKNNAHFIGDMETDMIAANRAGVKGHLIESNSNIVELAR
                     KICQHEVGVL"
BASE COUNT          432 a          272 c          203 g          441 t
ORIGIN      
        1 gaaatatcct gttctatagc actagtcagc tgaataataa ctttacgcat cacaccattc
       61 acacagcctg aagataatgg tgttgtataa accttattcc ctttcagcca aaacacatta
      121 gatgccgttg cttcacaaag catgtttttg ctatttaata tcagacagtc atcaaatttc
      181 atttcttgtg catatattga tgctaaaaca taaagaaggc tattacagtt tttaaatccg
      241 gaaagtttgt taattggttt ttgttgctca ctataaattc ctaattcaat aaaacactta
      301 ggtattgtat attcagattc tactgcagtt attggaagca tacatagtga ggcattgttc
      361 tgttcaggca ggtaagtgcc tccggcattt cgcgtaatca acaatcgtag tttgctgcca
      421 tctgtaaaat tatttctttg attcatctct aaaataattt cacgtagata atcttcagtt
      481 ataaaatcgc acttaagatt cagaacagca acccctgcaa gtaagcgttc aatatgtagt
      541 gacaaaaaat ttgctttacc cctactgcaa cgaatggatt caaaaagtcc atcgccataa
      601 ataaatcctc tattgtttag cttcagaacg ggagttgcgg catcaaccca ttgtccatca
      661 taatacgccc acttcatgct gacagatttt tcttgctaat tcaacaatat tgctgtttga
      721 ttcaatcaga tgacccttta ctcctgctct gtttgcagca atcatgtctg tttccatatc
      781 accgataaag tgtgcattat tcttatcaac cttataacgt gcaatggcct tttctaacaa
      841 caggctatcg ggtttgcggc aaaggcattt actcttctca ggatgatgtg ggcaataata
      901 ataatttgtt atagttatgc ttttcttctt aaattctgct gacataaatt gatgtactgc
      961 aagaacatca ttatgagtat aaactccttt tgcaatgcca ctttgattgg taacaacaaa
     1021 aattaaataa ccattgtcct gaaatatttt catagcatca aagatgccgt cattaaaaac
     1081 aacatcagca attttataag tataatacaa agtattgtca ttgattactc catctcggtc
     1141 aaagaaaatt gctttgtttg aattaatcat cagagcatac ctgttagttg atcaaagaaa
     1201 gtaagtatta atcccggtgc aaatgttatt ggaaaaacaa aaccatacac agcaccccaa
     1261 aaatgcgcat tatgattgat gttatctcct ccctttttat ccatgtaact cgacagccac
     1321 aaataaccta cacctgcaag aattccag
//
LOCUS       DAOOOL010000307         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_13599_length_1303_cov_24.124199, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000307 DAOOOL010000000
VERSION     DAOOOL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13599_length_1303_cov_24.124199"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKU82_12975"
     CDS             complement(<1..381)
                     /locus_tag="PKU82_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOZ83824.1"
                     /translation="MPIHFVYPFFLWFLAALSIPIIIHLFNFRRFKKIEFTNVRFLKE
                     IKEQTQSHSKLKHLLVLLMRLLALAFLILAFAQPFVPTKSTESSGQQTVSIYIDNSFS
                     MNLSGKEGSLLDEAKDKCRTIATSL"
     gene            478..1083
                     /locus_tag="PKU82_12980"
     CDS             478..1083
                     /locus_tag="PKU82_12980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOZ83825.1"
                     /translation="MLKSYFSDDVEAGIDEAGRGCLAGPVFAAAVILPRNFRNAELND
                     SKKLSALQRDNLRLVIEKEALAWAVAFCTHTEIDKINILQATYKAMHKAIAELKVFPE
                     RLLIDGNRFKKFKSVPHHCIVKGDGLFASIAAASVLAKTHRDEYMLNLHQKYPVYSWD
                     KNKAYATAVHVEAIKKYGLSSYHRKSFQLKSLQPQLIFPEN"
     gene            complement(1095..>1303)
                     /locus_tag="PKU82_12985"
     CDS             complement(1095..>1303)
                     /locus_tag="PKU82_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOZ83826.1"
                     /translation="NITEEEASSLVFTGKIENSAYTTSQENIHILFKDNVIKDIADAS
                     DNLSISALSKPVVKHYLCVDSGCL"
BASE COUNT          427 a          210 c          243 g          423 t
ORIGIN      
        1 gagtgaagtt gcaatggttc tgcatttatc ttttgcctca tcaagtaagg agccttcttt
       61 tccggaaaga ttcatgctaa aagaattatc aatataaata ctgacagttt gctgccctga
      121 tgattctgtt gactttgtcg gaacaaaagg ttgtgcaaaa gcaagaatca gaaaagctaa
      181 tgccaaaagt cgcattagca atacaagcag gtgctttaat ttagagtgcg attgtgtttg
      241 ttcttttatt tctttaagaa agcgcacatt ggtaaattca atttttttga atctcctgaa
      301 attaaacaga tgtataatga taggaataga aagtgctgcc agaaaccaaa gaaaaaacgg
      361 ataaacgaaa tgtattggca ttttttattg aggaacgcca caaatatact taaatatgca
      421 ccattttagt taaagttatg tgagtaatta aattaatgca attatttttg ctcagagatg
      481 cttaaatcat atttttctga tgatgtcgaa gccggtatag acgaagccgg aagaggttgt
      541 ctggcaggcc ctgtttttgc tgctgctgta attctccctc gtaatttcag gaacgctgag
      601 ttaaatgact caaaaaaact ttctgctttg caaagagata atttacgttt agtcattgaa
      661 aaagaggctt tggcatgggc tgttgcattt tgcactcata ccgagattga taaaattaat
      721 attttacagg caacatataa agcaatgcat aaggcaatag cagagttgaa ggtgtttcct
      781 gaaaggttac ttattgatgg caacaggttt aaaaaattta agtccgtacc tcatcattgt
      841 attgttaaag gtgatggtct gtttgcctcc attgctgctg catcggtact tgcaaaaaca
      901 catcgtgatg aatatatgtt aaatctgcat cagaagtacc ctgtatatag ttgggataaa
      961 aataaagcct atgctacagc agttcatgtt gaagcaataa aaaaatacgg cttatcatca
     1021 tatcatcgaa aatcgtttca acttaaatca ttacaacccc aattgatttt tcctgaaaat
     1081 tgaaatagtt ttttctaaag acaaccggag tcaacacaaa gatagtgttt cacaacaggt
     1141 ttactaagtg ctgaaatact cagattgtcc gaagcatcag caatatcttt aattacatta
     1201 tccttgaaaa gtatatgtat gttttcttga ctagttgtat atgcactgtt ttcgattttc
     1261 cctgtaaaaa caagacttga agcttcttcc tctgtaatgt tga
//
LOCUS       DAOOOL010000308         1271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_14134_length_1271_cov_38.645559, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000308 DAOOOL010000000
VERSION     DAOOOL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_1271_cov_38.645559"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKU82_12990"
     CDS             <1..291
                     /locus_tag="PKU82_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOZ83827.1"
                     /translation="LRSEANVLLLDEPTNDIDVNTLRALEEAIENFAGCAVIISHDRW
                     FLDRVCTHILAFEDDSQVYYFEGGYSDYEENKKKRLGDITPHRVRYKSLVKN"
     gene            313..>1271
                     /locus_tag="PKU82_12995"
     CDS             313..>1271
                     /locus_tag="PKU82_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOZ83828.1"
                     /translation="MKPSIPKGTRDFSPSEMVRRNFIFNTIKKVYERYGFVQIETPAM
                     ENLSTLEGKYGEEGDKLLFRILNSGEVVQDSKSKGENLKISDLAEKGLRYDLTVPFAR
                     YVVMHQNEISFPFKRYQMQPVWRADRPQKGRYREFYQCDADVIGSTSLISEAEFLLII
                     QEVFSSLQIDVAIKINNRKILAGIAEVIGHADKIIDITVAIDKLDKIGEQKVKEELLA
                     KGLSPDAVNKMQPLFESRGDFETKLNFLKTFLAESSIGLKGIEETENVISYLNAFGKQ
                     LKNLELDITLARGLNYYTGSIFEVKVNDSRSTFSGSVLGGGRY"
BASE COUNT          415 a          217 c          276 g          363 t
ORIGIN      
        1 ttacgaagcg aagccaacgt gttgttactt gatgagccta ccaacgacat tgatgtaaac
       61 acattgcgtg ccttagaaga agcaatcgag aattttgcag gctgtgcagt tatcatcagc
      121 cacgacagat ggtttttaga tagggtatgt acacacattc ttgctttcga agacgactcg
      181 caggtatatt atttcgaagg cggatattcc gactacgaag agaataagaa aaaacgattg
      241 ggcgatatca ctcctcatag agtgaggtat aaaagtttgg taaaaaacta gattcgtaat
      301 tcgcagcacc aaatgaaacc atccatcccc aaaggcactc gcgatttttc accatcagaa
      361 atggtacgca gaaactttat tttcaatacc atcaaaaagg tatatgagcg ctatgggttt
      421 gtacagattg aaacacctgc aatggaaaac ctttcaacac ttgaaggtaa gtatggtgag
      481 gaaggtgata aacttttatt cagaatattg aattcaggcg aagttgttca ggattcaaag
      541 tcaaaaggtg aaaatcttaa aatttctgat ttggcagaga aaggcttgcg atatgattta
      601 acagttccgt ttgcacgtta tgtagtcatg catcagaatg aaatcagctt tccgtttaaa
      661 cgttatcaga tgcaaccggt atggcgtgcc gaccgtccac agaaaggacg ttatcgtgaa
      721 ttttatcagt gcgatgccga tgttattggt tcaacttcat taatcagcga agcagaattt
      781 cttctaatca ttcaggaagt gttcagcagt ttgcagattg atgttgccat aaagataaat
      841 aacagaaaaa ttctggcagg cattgccgaa gttattggtc atgccgataa aataattgac
      901 attaccgttg ccattgataa actcgataag ataggagaac agaaagttaa ggaagaactt
      961 ttggctaaag gactttcgcc tgatgcggta aataaaatgc aaccgctttt tgaatcgcgc
     1021 ggtgattttg aaacaaaact caacttcctg aaaacatttt tggctgaatc ttcaatagga
     1081 ttaaaaggta ttgaagagac agaaaatgtt ataagttatc ttaatgcttt tggaaagcaa
     1141 ctgaaaaatc ttgaattaga cataaccctt gcacgtgggc ttaattatta tacaggttca
     1201 atttttgaag tgaaggttaa tgactcgcgt tcaactttca gtggcagtgt gcttggtggt
     1261 ggacgctatg a
//
LOCUS       DAOOOL010000309         1238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_14705_length_1238_cov_41.712595, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000309 DAOOOL010000000
VERSION     DAOOOL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14705_length_1238_cov_41.712595"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PKU82_13000"
     CDS             <1..823
                     /locus_tag="PKU82_13000"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOZ83829.1"
                     /translation="ILNAALQRNLFMDASVLVLEDQAKTVVQNEREMHGDFKVVVAKL
                     NQSSTYKKIFSQAFAGTKDTAISEEGILKALAEFQRTQLSFDSPFDKAMRGAGTIEQP
                     VIDGYNIFMGKAKCGHCHFAGLFNGTTPPQYRESEFDIIGVPSTNKRPYKLDGDLGRY
                     NVFKNDELKYAFKTTTVRNIAVTAPYMHNGVYKTLQEVVDFYDKGGGAGLGIHLPDQT
                     LPDDKLKLTKDEKKKLILFLQSLTDTSAVVHFSGQLPAIDNSASPLNKRIAGGTD"
     gene            872..>1238
                     /locus_tag="PKU82_13005"
     CDS             872..>1238
                     /locus_tag="PKU82_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOZ83830.1"
                     /translation="MYNLLKGKRGIIFGALDENSIAWKTAQRAHQEGAMFTLTNAPVA
                     MRMGEINKLAAECNAQVIPADATSIEDLENLFTKSMEILGGKLDFVLHSIGMSPNVRK
                     GKHYTALDYEYFHKTLDVSA"
BASE COUNT          393 a          264 c          254 g          327 t
ORIGIN      
        1 aattttaaat gctgcattgc agcgcaatct gtttatggat gccagtgtat tggttctcga
       61 agatcaggca aaaactgttg ttcagaatga gagagagatg catggcgatt ttaaagttgt
      121 cgttgccaaa ctcaatcagt cttcaaccta caaaaaaata ttcagtcagg cttttgcagg
      181 aacaaaagat actgccataa gcgaagaagg cattctaaaa gcattggcag aatttcagcg
      241 cacacaactg tcgttcgatt cgccttttga taaagccatg cgtggtgcag gcacaataga
      301 acaacctgtc attgatggct acaatatttt tatgggcaaa gccaaatgcg ggcactgcca
      361 ttttgcagga ctctttaacg gaaccacacc gccacagtac agagaaagtg agtttgatat
      421 tataggtgtg ccctcaacca acaaacgtcc ttataaatta gatggcgacc ttgggcgcta
      481 caatgttttt aaaaatgacg agcttaaata tgcattcaaa acaactactg tccgtaacat
      541 tgcagtgact gcaccctaca tgcacaacgg agtttacaaa acgcttcagg aggttgttga
      601 tttttatgac aaaggaggtg gggcagggct gggcattcac cttccggacc aaacccttcc
      661 cgatgataaa ttaaaactca ctaaagacga aaagaaaaag ctgattctgt ttctgcaatc
      721 gctcaccgat acttctgccg ttgtgcactt tagcggacag ttgcctgcca ttgacaatag
      781 tgcttcgcca ctgaataaac gcattgccgg tggcacagat taatgcagtt tcatcaattt
      841 ctattacttt agccgcaaaa caacataact tatgtacaat ttgttaaaag gaaaacgtgg
      901 aatcattttt ggcgcattag acgaaaactc tattgcatgg aaaaccgcac aacgtgcaca
      961 tcaggaagga gcaatgttta cattaaccaa cgctcctgtt gctatgcgca tgggcgaaat
     1021 aaacaaactt gcagcagaat gtaatgcgca agtcattccg gcagatgcca cttctattga
     1081 agacctcgaa aacctgttta ccaaatcaat ggaaatttta ggcggtaaat tagatttcgt
     1141 tttacacagc attggcatgt cgcccaatgt ccgtaaaggc aaacattaca cagcattaga
     1201 ctacgaatat tttcataaaa cattggatgt ttctgcac
//
LOCUS       DAOOOL010000310         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_15284_length_1207_cov_43.993056, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000310 DAOOOL010000000
VERSION     DAOOOL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15284_length_1207_cov_43.993056"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1145
                     /gene="queA"
                     /locus_tag="PKU82_13010"
     CDS             96..1145
                     /gene="queA"
                     /locus_tag="PKU82_13010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOZ83831.1"
                     /translation="MKLSQFKFGLNENLLAPHPAANRDESRLMVIDRKTGKIEHKIFK
                     DIINYFDEGDVMVMNNTKVFPARLYGNKEKTGAKIEVFLLRELNPESKLWDVLVDPAR
                     KIRIGNKLYFGEDDMLVAEVIDNTTSRGRTLRFLFDGTSADFREVIEKLGNTPLPKYI
                     KRKAEPEDKERYQTVYAKNEGAVAAPTAGLHFSKQLLKRLEIKGINLAELTLHVGLGT
                     FRPVEVEDLTKHKMDSEWFSITQPCVDIVNKAKAGKHRICAVGTTSMRAMESSVSTLG
                     ELKPFEGWTNKFIFPPYDFSLANCMVTNFHMPESTLLMMVSAFAGYDLTMHAYKLAMK
                     EKYRFYSYGDAMLII"
BASE COUNT          382 a          232 c          247 g          346 t
ORIGIN      
        1 tttgatatat agttagttac aacttttttt cgccatcggc ttctgcttgg tatatatttg
       61 cgccctcaaa aaaataatcc tgtaagatta cagatatgaa attatcacaa tttaaattcg
      121 gcctaaacga aaaccttctt gctccacatc ctgctgccaa ccgtgacgaa agtcggttga
      181 tggttattga ccgtaaaacc ggtaagattg agcataaaat atttaaagac atcattaatt
      241 actttgacga gggcgatgtg atggtgatga ataacaccaa ggtttttcct gcacgccttt
      301 atggcaataa agaaaagacg ggtgctaaaa ttgaagtctt tttattacgt gaactaaatc
      361 cggaaagcaa actttgggat gtgcttgttg accctgcacg taaaatacgt attggtaata
      421 aattgtattt cggtgaagat gatatgcttg ttgccgaagt aattgataac acaacttcac
      481 gtggtcgtac tttgcgcttc ttatttgatg gcacaagtgc cgatttcaga gaggttattg
      541 agaagttagg aaatacacca ctcccgaaat atatcaaacg aaaggccgag cccgaagata
      601 aagagcgcta ccagacagta tatgccaaaa acgaaggtgc tgttgcagca cccactgccg
      661 gactacactt cagcaaacaa ttattaaagc gcctagaaat taaaggcatc aaccttgctg
      721 aactgacact gcatgttggt ttaggcacat tcagaccggt agaagtagaa gacctaacca
      781 aacataagat ggattctgaa tggttcagta ttacacaacc ttgtgttgac atagttaata
      841 aagccaaagc aggcaaacac cgtatttgtg cagtaggaac aacgtcaatg cgcgcaatgg
      901 agtcatcagt ttccacatta ggtgagctta aaccttttga aggctggaca aataaattta
      961 tttttccacc ttatgatttc agccttgcca attgtatggt gactaatttt cacatgcctg
     1021 aatcaacttt gttgatgatg gtttctgctt ttgcaggtta cgaccttacc atgcatgctt
     1081 acaaattagc gatgaaagaa aaataccgct tctatagcta tggcgatgcc atgctgataa
     1141 tctgacagtt tatttattat gagccaaaag cgatttgcaa taatagttgc cggaggcagt
     1201 ggaacac
//
LOCUS       DAOOOL010000311         1172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_15998_length_1172_cov_30.966876, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000311 DAOOOL010000000
VERSION     DAOOOL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15998_length_1172_cov_30.966876"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKU82_13015"
     CDS             complement(<1..890)
                     /locus_tag="PKU82_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83832.1"
                     /translation="MSNRKIVLSLLAFLIVIYSLLRLSFIIINLSSFSLSSPAEFLMV
                     LFAGLRFDLSAIFITNVLIAVILLLPFKFHRKSVKIVITTLFLLGNLPFLLFNVVDVG
                     YYHFTQKRTTADFFGVATMGDDFFNMLPSLLRDYWGLLVTFLILSVGLYKAFQWFLNR
                     RTGKSKWWFAEIVVTIGLTVLLFRGGLQYKPISVLTAARYTSNQNVAAILNTTFTILK
                     TLGKDELKNYNFDKKIPSALTYFNPMHNYANSGVFRRMNVVLIIMESFGKEYTGYFNQ
                     GKGYTPFLDSLMQHSLTCTDA"
BASE COUNT          421 a          204 c          221 g          326 t
ORIGIN      
        1 gcatcagtac aggttaaact gtgctgcatc aaagaatcta aaaatggtgt gtaacccttg
       61 ccctgattaa aatagcctgt atattccttc ccaaaactct ccatgatgat taatacaaca
      121 ttcattcgtc taaatactcc tgaatttgca tagttatgca tgggattaaa atatgtcaat
      181 gcagaaggaa tttttttgtc aaagttgtag ttcttgagtt catcttttcc aagtgttttt
      241 aggatagtaa atgtggtgtt gagaatggca gctacattct gattgctcgt ataacgtgca
      301 gcagtaagca cagaaattgg tttgtattga agtccgcctc tgaaaagcag aacggttaaa
      361 cctatagtca caacaatttc tgcaaaccac catttgcttt ttcctgttct acggtttaaa
      421 aaccattgaa atgccttgta aagtccaaca gaaagaatca gaaatgtgac taacaacccc
      481 caatagtcac gtaacaatga cggcaacata ttgaagaaat cgtcacccat ggtagctaca
      541 ccaaaaaaat cggctgtggt gcgtttttga gtaaaatgat aataaccgac atctactacg
      601 ttaaaaagca gaaatggcag gttgccaagc aagaataagg ttgttattac aattttcaca
      661 gatttccgat gaaatttgaa tggcaacagt aatatgacgg caataagtac gtttgtaata
      721 aaaatagcgc tgaggtcaaa gcgaagaccg gcaaaaagca ccattaaaaa ttctgccggg
      781 ctactgagac tgaaagaact aaggtttatt ataataaaag aaaggcgtaa taacgaatat
      841 atgacaatga ggaatgccaa taacgacaat acaatttttc tgttcgacat gcagagggca
      901 aatataactt ataggctttg taacctatag aagcaggtgg ttttagtgtc aataaccatt
      961 aactttaagc tgttaatatc taatcttgaa aaaatattga aaacctgaca attaaagtat
     1021 taattttgcg acaaatttca atcaattcaa aataaaaatg aaaaaatcac tactagcgct
     1081 atcagtagca ggtatgtttc tgctgagcgc ctgcggacca agcgcagatg aaaaagccgc
     1141 tgaggcaaaa agagtacaag attctattga tg
//
LOCUS       DAOOOL010000312         1161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2624
            NODE_16227_length_1161_cov_91.832731, whole genome shotgun
            sequence.
ACCESSION   DAOOOL010000312 DAOOOL010000000
VERSION     DAOOOL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864699
            Sequence Read Archive: SRR4244875
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:29:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,605
            CDSs (total)                      :: 2,572
            Genes (coding)                    :: 2,551
            CDSs (with protein)               :: 2,551
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16227_length_1161_cov_91.832731"
                     /isolate="AMR_MDS_2624"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKU82_13020"
     CDS             complement(<1..420)
                     /locus_tag="PKU82_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ83833.1"
                     /translation="MQKYALKVRQRLADYISLPDKTKVTVMQQQKCLNFFILFILSCL
                     FASDIEAQQYKFAKRVGGTQFENAMGIALDSTGNIIVSGYFSGTADFDPSTSNYSLTS
                     NGGNDIFLARYDSAGNFIWAINVGGAGEEFNYSDPAVD"
     gene            426..>1161
                     /locus_tag="PKU82_13025"
     CDS             426..>1161
                     /locus_tag="PKU82_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOZ83834.1"
                     /translation="MDHSTKTFALSNGLQIVLTTLSNTEVSHCALLIKAGTRNEQIQQ
                     EGLAHFIEHMLFKGTRKRKSFHILNRLEVVGGELNAFTSKEETCIHASVTTPHLERAL
                     ELISDITFNSVFNLKEIEKEKEVVCDEIRSYLDNPGEQIFDDFESLVFKKNPLGNPIL
                     GTEQSVKSFKQKDLIAFTRKFYIPSNMVLSIATSLNAEKVIRQLEHHFGKYRLAKVGE
                     RPKPFKKYTAEKSSVKKHSAQCHFIMG"
BASE COUNT          417 a          223 c          190 g          331 t
ORIGIN      
        1 atcaacagca gggtctgagt aattaaactc ctctcctgct ccacctacat taattgccca
       61 tataaaatta cctgcactat catatctagc aagaaaaatg tcattaccac cattgcttgt
      121 taaagaataa ttgcttgtgc tcggatcaaa atctgcagtt cctgaaaaat aacctgacac
      181 aataatattt cctgtgctat ctaatgcaat ccccattgca ttctcgaatt gtgttcctcc
      241 tacacgtttg gcaaacttat attgctgcgc ctcaatatca gaagcaaaca gacaacttaa
      301 aatgaataaa ataaaaaaat tcaggcattt ttgctgttgc ataacagtta cttttgtttt
      361 atcaggtaaa gatatgtaat cagccaatcg ttgtctgact ttcaaagcat atttttgcat
      421 aaactatgga tcattcaaca aaaacttttg cactatcaaa cggactacag atagttctta
      481 caactttaag taacacagaa gtctcgcatt gtgcattact cataaaagcc ggaacaagaa
      541 atgagcaaat acaacaagaa ggattagcgc atttcataga gcacatgctg ttcaagggaa
      601 cacgaaagcg taaatcgttt catatcctaa atcgtcttga agtcgtaggt ggtgagctaa
      661 atgcattcac cagcaaggaa gagacatgta ttcatgcatc agttaccaca cctcatcttg
      721 aacgtgcact tgaacttatt agtgatatca cctttaattc tgttttcaat ttaaaagaaa
      781 tagaaaaaga aaaggaagtt gtttgtgatg aaataaggtc ttatctcgac aatccgggag
      841 agcagatttt tgatgacttt gaaagcttag tttttaaaaa aaatccatta ggaaatccaa
      901 tcttaggtac agaacaatca gtaaaaagtt ttaagcagaa agacctgatt gcgtttacac
      961 gaaagttcta cattccatcc aacatggtgc tatcaattgc cacatcatta aatgcagaaa
     1021 aagtaatcag gcaattagaa catcatttcg gcaagtacag acttgccaaa gtgggcgaaa
     1081 gaccaaaacc atttaaaaaa tacactgctg aaaagtcatc tgttaaaaaa cacagtgcac
     1141 aatgtcattt tattatgggg a
//
